From malbenatti <@t> googlemail.com Thu Apr 1 00:32:18 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Thu Apr 1 00:32:13 2010 Subject: [Histonet] cassette labels erased by processor In-Reply-To: References: Message-ID: <9793966B-64F1-4D1A-9448-6B9859DD3AFE@gmail.com> Another option would be to invest in a cassette labeling machine, they are few a available in the market, we use the Surgipath Cassette Marker, they are pretty good as the ink fix with formalin and survive processing. Though ribbon need to keep refrigirated when not in use(overnight and weekend) and printing heard need to be clean at least once a week, but apart from tha it is pretty straight forward to use, also it can be program so it make printing a large number of cassette painless . I have also used in tha past cassette marker that use thermal print ribbon so again no risk of lab number not surviving processing.So if you lab as some spare cash to spend get few on trial in, az they are worth it. Best wishes, Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London " ... Smile it confuses people ..." On 1 Apr 2010, at 00:24, Amos Brooks wrote: > Indeed, > One of the most EVIL products ever designed is the "Permanent > Lab Marker" by VWR. Talk about false advertising! We have > researchers drop off buckets of cassettes that they have labeled > using these wonderful products. Pencil is the ONLY acceptable means > of labeling cassettes. It is just not worth the risk to use anything > else. (Except cassette labelers they are specifically designed for > this and have a sales rep you can beat up if they fail.) Even pens > specifically designed for cassettes have failed at times. > > Just my $0.02, > Amos > > > Message: 23 > Date: Wed, 31 Mar 2010 15:40:44 +0100 > From: Malika Benatti > Subject: Re: [Histonet] cassette labels erased by processor > To: Catherine Breen > Cc: Histonet List > Message-ID: > Content-Type: text/plain; charset=us-ascii; > format=flowed; delsp=yes > > Hi there, > I would suggest to use a pencil rather than a marker pen to label > cassettes when processing cassette with the Sakura VIP , my lab had a > number of the so called solvent proof pen on trial and have yet to > find one that survive processing. > > Malika Benatti BSc MIBMS > Specialist Biomedical Scientist > Great Ormond Street Children Hospital > London From alexandra.meinl <@t> gmail.com Thu Apr 1 04:00:22 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Thu Apr 1 04:00:31 2010 Subject: [Histonet] Assay for cell death needed Message-ID: Hello Histonetters, We desperately need an assay that detects cell death in human FFPE sections (regardless if its apoptosis or necrosis). Some kind of kit (quick 'n easy) would be really fine! Any suggestions? Many thanks, Alexandra ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From leiker <@t> buffalo.edu Thu Apr 1 08:02:48 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Apr 1 08:04:44 2010 Subject: [Histonet] mouse perfusion rate In-Reply-To: <619868.61085.qm@web113116.mail.gq1.yahoo.com> References: <619868.61085.qm@web113116.mail.gq1.yahoo.com> Message-ID: <807E6E9C4F3C8A712B8F0F92@CDYwxp1931.ad.med.buffalo.edu> Someone I believe on Histonet a couple years ago told me the same thing. The "perfusion circuit" is compromised if you have fluid coming out the nose and internal organs swelling up... Merced --On Wednesday, March 31, 2010 4:14 PM -0700 "Andrea T. Hooper" wrote: > > Very interesting! Coming out the nose is definitely bad for any work I > have done in the past - lungs get blown out, liver doesn't perfuse well > and bone marrow looks horrific. However, if you are working with PFA and > doing a post-fix anyway, you will probably be fine. If you are using GA > and counting on the perfusion to ensure excellent fixation for things > such as lacZ staining (b/c post-fix in GA never works well for bone or > deep into tissues) then blowing it out the nose is bad. Very bad. > > Andrea > > > --- On Mon, 3/29/10, Charles.Scouten@leica-microsystems.com > wrote: > > > From: Charles.Scouten@leica-microsystems.com > > Subject: RE: [Histonet] mouse perfusion rate > To: leiker@buffalo.edu, saby_joseph_a@yahoo.com, making@ufl.edu, > histonet@lists.utsouthwestern.edu > Date: Monday, March 29, 2010, 6:26 PM > > > I have perfused mice and rats at 300 mm Hg, about double physiological > level, don't know what that made the flow rate. All mammals have the > same blood pressure (within tolerances), so it is easier to select a > suitable pressure to use than a flow rate, which varies dramatically. > > > > I look at brain, never pay any attention to the gut. Clear fluid comes > out the nose, that is a good sign. There are pressure release valves > across the cribiform plate to release CSF if there is too much. I am > flooding the system, fluid coming out the nose means the extracellular > fluid and CSF is being replaced as well as vascular blood. Good. > > > > The tissue is quality is excellent, free of red blood cells, can be > unshrunk depending on the tonicity (should be sub isotonic) of the > fixative fluid. Have looked at Nissl and EM material, no evidence of > damage to the tissue. > > > > If gut is extended, might have something to do with the large intestines > job of removing fluid from feces, and flooding the system swells the > tissue. But does it matter? Do you use that tissue? What is the > tissue quality if you use it after physiological pressure perfusion. > > > > Cordially, > > Charles W. Scouten, Ph.D > > Product Manager, MNL > > Biosystems Division > > > > Leica Biosystems Richmond, Inc. > 5205 Route 12 > P.O. Box 528 > Richmond, IL 60071 > United States of America > > Telephone 630 964 0501 > > facsimile +1 630 964 0576 > > www.MyNeuroLab.com > > www.leica-microsystems.com > > > > IMPORTANT - This email and any attachments may be confidential. Any > retransmissions, dissemination or other use of > > these materials by persons or entities other than the intended recipient > is prohibited. If received in error, please contact > > us and delete all copies. Before opening or using attachments, check > them for viruses and defects. Our liability is limited > > to resupplying any affected attachments. [Any representations or > opinions expressed in this email are those of the > > individual sender]. > > > > > > From: Merced M Leiker [mailto:Merced M Leiker > ] > Sent: Monday, March 29, 2010 9:05 AM > To: Joseph Saby ; > Charles.Scouten@leica-microsystems.com; making@ufl.edu; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] mouse perfusion rate > > > > Hi Joe, > > Thanks for that notice about flow rates. But I think for the mouse you > meant 1-3mls/min (not per 10min?)... > > Regards, > Merced > > --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby > < saby_joseph_a@yahoo.com> wrote: > >> >> >> All- >> >> From previous work with rat perfusions, the flow rate was about 10 >> ml/minute. If I had to guess, the equivalent flow rate for a mouse > would >> be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will >> definitely cause blowout artefacts. >> >> Joe Saby, BA HT >> >> >> >> >> __________________________________________________ >> From: Merced M Leiker < leiker@buffalo.edu> >> To: Charles.Scouten@leica-microsystems.com; making@ufl.edu; >> histonet@lists.utsouthwestern.edu >> Sent: Fri, March 19, 2010 9:21:38 AM >> Subject: RE: [Histonet] mouse perfusion rate >> >> The vasculature will leak too much and the mouse will get bloated - >> you'll >> see it first in either the intestines blowing up like a balloon or > fluid >> coming out of the nose. Just not the same as the heart pumping when > the >> mouse is alive with intact physiology and normal functioning. Don't > know >> exactly why, but that's what happens when you go too fast. Perhaps the >> vasculature has lost its control to compensate for the pressure? I'm > not >> a >> physiologist so I'm not sure why...maybe someone on the Histonet can >> answer >> that? >> >> Regards, >> Merced >> >> --On Thursday, March 18, 2010 5:49 PM -0500 >> Charles.Scouten@leica-microsystems.com wrote: >> >>> >>> >>> Why not? What happens? One would think the mammalian cardiovascular >>> system could withstand physiological pressures and flow rates, at > least >>> for one lifetime? >>> >>> >>> >>> >>> Cordially, >>> >>> Charles W. Scouten, Ph.D >>> >>> Product Manager, MNL >>> >>> Biosystems Division >>> >>> >>> >>> Leica Biosystems Richmond, Inc. >>> 5205 Route 12 >>> P.O. Box 528 >>> Richmond, IL 60071 >>> United States of America >>> >>> Telephone 630 964 0501 >>> >>> facsimile +1 630 964 0576 >>> >>> www.MyNeuroLab.com >>> >>> www.leica-microsystems.com >>> >>> >>> >>> IMPORTANT - This email and any attachments may be confidential. Any >>> retransmissions, dissemination or other use of >>> >>> these materials by persons or entities other than the intended > recipient >>> is prohibited. If received in error, please contact >>> >>> us and delete all copies. Before opening or using attachments, check > them >>> for viruses and defects. Our liability is limited >>> >>> to resupplying any affected attachments. [Any representations or > opinions >>> expressed in this email are those of the >>> >>> individual sender]. >>> >>> >>> >>> >>> >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Merced M >>> Leiker < leiker@buffalo.edu> >>> Sent: Thursday, March 18, 2010 12:38 PM >>> To: MKing < making@ufl.edu>; histonet@lists.utsouthwestern.edu >>> Subject: Re: [Histonet] mouse perfusion rate >>> >>> >>> >>> That may be mouse cardiac output, but I can assure you, from > experience, >>> you do not want to perfuse at 17ml/min. >>> >>> Regards, >>> Merced >>> >>> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> >>> wrote: >>> >>>> Li, >>>> >>>> Mouse cardiac output seems to be about 17 ml/min (e.g. >>>> www.transonic.com/mice1.shtml), you probably want to try for that to >>>> keep pressures close to physiological. >>>> A syringe pump is pretty inexpensive and probably all you need. >>>> >>>> Mike >>>> >>>> ----- Original Message ----- >>>> From: Li Zhang < dancingwing@yahoo.com> >>>> Date: Wednesday, March 17, 2010 14:59 >>>> Subject: [Histonet] question about mouse perfusion >>>> To: histonet@lists.utsouthwestern.edu >>>> >>>> > > My question is: can anyone give me a rough idea of how fast I >>>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >>>> > > min, and I suspect that it's too fast because I do observe >>>> > > tissue swelling sometimes. >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> Merced M Leiker >>> Research Technician III >>> Cardiovascular Medicine >>> 348 Biomedical Research Building >>> State University of New York at Buffalo >>> 3435 Main St, Buffalo, NY 14214 USA >>> leiker@buffalo.edu >>> 716-829-6118 (Ph) >>> 716-829-2665 (Fx) >>> >>> No trees were harmed in the sending of this email. >>> However, many electrons were severely inconvenienced. >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > ______________________________________________________________________ >>> This email has been scanned by the MessageLabs Email Security System. >>> For more information please visit http://www.messagelabs.com/email >>> > ______________________________________________________________________ >> >> >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Apr 1 08:35:28 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Apr 1 08:37:06 2010 Subject: [Histonet] DAKO Her2 antibody In-Reply-To: <95D667DD0E6A41B0BABCA40660B5BF29@lurie.northwestern.edu> References: <95D667DD0E6A41B0BABCA40660B5BF29@lurie.northwestern.edu> Message-ID: Do you bake your slides? How old are your cut sections that you are testing? We have found that long baking of the sections before staining? Like overnight at 60C causes the staining intensity to decrease. And if the sections are older than a month the staining intensity decreases as well. Just a thought. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick [b-frederick@northwestern.edu] Sent: Wednesday, March 31, 2010 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Her2 antibody Anyone out there noticing problems with the Her2 antibody for Dako? Seems real strong and deteriorating quickly. We order it in lots, so you can imagine... Seems like every run is different. Same stainer, same titre , same tech. Tissue is from all over the country and world so we cannot control fixation etc. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Apr 1 09:42:33 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 1 09:42:39 2010 Subject: [Histonet] FW: New CAP grossing guidelines Message-ID: That would be my guess; I have on file copies of degree and course study for my grossing personnel Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, March 31, 2010 2:44 PM To: Histonet Subject: [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From andreahooper <@t> rocketmail.com Thu Apr 1 10:31:15 2010 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Thu Apr 1 10:31:20 2010 Subject: [Histonet] Assay for cell death needed In-Reply-To: Message-ID: <653804.54705.qm@web113104.mail.gq1.yahoo.com> Use the TUNEL assay, several very easy to use kits available. Roche's Cell Death Detection kit with FITC might be the easiest. --- On Thu, 4/1/10, Alexandra Meinl wrote: From: Alexandra Meinl Subject: [Histonet] Assay for cell death needed To: "Histonet" Date: Thursday, April 1, 2010, 9:00 AM Hello Histonetters, We desperately need an assay that detects cell death in human FFPE sections (regardless if its apoptosis or necrosis). Some kind of kit (quick 'n easy) would be really fine! Any suggestions? Many thanks, Alexandra ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel:? +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Thu Apr 1 10:38:15 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Apr 1 10:38:35 2010 Subject: [Histonet] Pig tissue IHC detection Message-ID: <4BB47797020000C5000747BB@mail.TSRH.ORG> I just wanted to know if anybody who are working with Pig bone tissue fix in 10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit for mouse monoclonal and rabbit polyclonal antibodies. I am using a Powervision kit from Immunovision, now Leica and I have a lot of background staining even with my negative stain. Please help me remedy the background stain.Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From JMacDonald <@t> mtsac.edu Thu Apr 1 11:11:26 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 1 11:12:06 2010 Subject: [Histonet] Pathologist needed for NAACLS Message-ID: The National Accrediting Agency for Clinical Laboratory Sciences is looking for a Pathologist to sit on a committee. The committee is the Review Committee for Accredited Programs (RCAP). The committee meets twice a year, once in person and once via a lengthy teleconference (day). The face-to-face meeting is in Chicago or the surrounding area. The teleconference meeting is held in February and the in- person meeting is in July. This is a volunteer position, but NAACLS does cover all travel costs associated with the meeting. They also feed you very well. The RCAP is comprised of 22 members. To ensure appropriate representation from the professions of clinical laboratory science, pathology and education, the composition of the CLSPRC includes: five CLS/MT educators; five CLT/MLT educators; two HT/HTL educators; one clinical pathologist; one anatomic pathologist; two pathologists? assistant educators or practitioners; two cytogenetic technology educators or practitioners; two DMS educators; one four-year educator generalist; and, one two-year educator generalist. Ideally the candidate will have experience with the accrediting process and have some time to devote to reviewing documents related to program accreditation. The document review usually takes place a month or two before each meeting. There are also small sub-committees. Initially the committee member will be paired with an experienced member of the committee. If you are interested in this position or know a great pathologist that would be interested please contact me and I will send you more information regarding the position. NAACLS would like to fill this position as soon as possible, so that the pathologist is familiar with the process before the July meeting. Thank you, Jennifer MacDonald Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From Kim.Donadio <@t> bhcpns.org Thu Apr 1 11:15:51 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Apr 1 11:16:10 2010 Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A044@EXCHMBC2.ad.ah.local> Message-ID: I agree Janice. The only thing it looks to me is to now define Grandfathered in. Because it also states "in addition" for that criteria as well. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mahoney,Janice A" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2010 04:10 PM To 'Joe Nocito' , Histonet cc Subject RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines But above that after the education piece it says "in Addition". Jan, Omaha -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, March 31, 2010 4:08 PM To: Mahoney,Janice A; Histonet Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the "processing" and "grossing" pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe ----- Original Message ----- From: "Mahoney,Janice A" To: "Histonet" Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From jkiernan <@t> uwo.ca Thu Apr 1 11:42:53 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Apr 1 11:42:59 2010 Subject: [Histonet] mounting media Message-ID: I've used both the cytoseal mountants (with numbers that indicate viscosity) for several years. These poly(methylmethacrylate) media come in very convenient plastic squeezy bottles for application. They are less expensive than entellan, the classic polymethacrylate mountant, which has to be dispensed from its bottle with a glass rod. With cytoseal mounting media, lines of little bubbles frequently form, very near the edges of the coverslip. I don't know why. Bubbles at the edge don't matter if the sections are small and centrally located on the slide. I've not seen these lines of bubbles with entellan or with DPX, a non-fluorescent polystyrene medium. John Kiernan Anatomy, UWO London, Canada = = = -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tigger13b@aol.com Sent: Wednesday, March 31, 2010 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MM24 mounting media Hello, Does anyone use MM24 mounting media from surgipath? We use cytoseal 60 but I can get the MM24 at a lower price. If anyone has used this, could you tell me whether you were satisfied with the product or not? Thanks so much. Brandi Higgins _______________________________________________ From jkiernan <@t> uwo.ca Thu Apr 1 11:57:03 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Apr 1 11:57:08 2010 Subject: [Histonet] Assay for cell death needed In-Reply-To: References: Message-ID: Dead cells stain intensely with anionic dyes such as eosin. For nervous tissue there's a simple method using acid fuchsine and toluidine blue. Normal neurons are blue; dead and dying ones are red. See Auer RN et al 1984 Acta Neuropathologica 64: 177-191. Also Biotechnic & Histochemistry 73: 244-254 (1998). The second paper includes an investigation of why the nuclei and cytoplasm of moribund cells stain red. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Alexandra Meinl Date: Thursday, April 1, 2010 5:01 Subject: [Histonet] Assay for cell death needed To: Histonet > Hello Histonetters, > > We desperately need an assay that detects cell death in human > FFPE sections > (regardless if its apoptosis or necrosis). > Some kind of kit (quick 'n easy) would be really fine! > > Any suggestions? > > Many thanks, > > Alexandra > > ************************************************ > Dr. Alexandra Meinl > Ludwig Boltzmann Institute > for Experimental and Clinical Traumatology > Austrian Cluster for Tissue Regeneration > Histology > Donaueschingenstrasse 13 > 1200 Vienna - Austria > > Contact @ Bernhard Gottlieb University School of Dentistry, > Waehringerstr. 25a, A-1090 Vienna > tel: +43 1 4277 67026 > fax: +43 1 4277 67019 > email: alexandra.meinl@trauma.lbg.ac.at > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From napoli <@t> siscom.net Thu Apr 1 12:26:42 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Apr 1 12:26:45 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Message-ID: <4bb4d752.23.7e05.848022978@siscom.net> Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with these subjective entities? The BS I have heard over the last few months concerning MOHS surgery specimens is one glaring example of the limitations CAP has in understanding fully certain nuances of the lab trade. Ridiculous. Unless you want the marketing and potential "perception" that you are better covered from a legal standpoint, CAP certs are worthless. The more I hear about CAP certifications, the more I see it as a certain community of individuals who are protecting their perceived "TURF." In the end, the pathologists in the group and in the facility in which you are working have to take responsibility for these matters. If the docs think a CAP cert is necessary, then do it and live with it. If not, then consider yourself lucky to not have to see these people in your lab. I have been through MANY CAP inspections in and out of the military. For the most part, though, I see people paying this organization to inspect their lab as the same thing as "burning a pinch of incense in honor of great Caesar, ruler of Rome." It will get you some kudos, but tangibly not change much at all if your pathologists or HR $ hiring hands want to pocket more $ as a result of hiring "pregnant out of wedlock 16 year olds" to gross tissue and cut slides. Seen it. AB From napoli <@t> siscom.net Thu Apr 1 12:47:20 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Apr 1 12:47:25 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Message-ID: <4bb4dc28.296.c8a.1494624254@siscom.net> Correction...I meant to include "High school drop-out" in my example. Furthermore, this is NOT a gender based comment...just a real life example of some of the things I have seen in "CAP" or "non-CAP" inspected laboratories. Regards, AB From Lori.Disher <@t> HCAhealthcare.com Thu Apr 1 13:01:35 2010 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Thu Apr 1 13:01:43 2010 Subject: [Histonet] cassette labels erased by processor Message-ID: <778DD853CF606049A37FC2059C8BA07A62A5D6346E@FWDCWPMSGCMS04.hca.corpad.net> We use "Sharpie, Industrial Super Permanent Ink, extra fine point" without any trouble Lori A Disher Fawcett Memorial Hospital From tjasper <@t> copc.net Thu Apr 1 13:46:23 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Apr 1 13:46:31 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com References: <4bb4d752.23.7e05.848022978@siscom.net> Message-ID: <90354A475B420441B2A0396E5008D49692BF2E@copc-sbs.COPC.local> I'm inclined to agree with you Andrew. Seems to me that CAP has become, unintentionally (I'd like to believe) something of an unsavory, bullying sort of entity. I'm not certain about all the factors involved, but I think a few things have definitely contributed to all of this CAP negativity. First of all - CAP was (is?) considered the accrediting "gold standard". That's pretty heady stuff...possible ego inflation potential...high horse attitude...elitism? Not saying that was the plan, just an unfortunate and unintended consequence. I'm sensing a power trip here, it's partly human nature (I guess) and it can definitely suck! Secondly - as I recall, CAP got egg on their face a few years back in the DC/Baltimore area I believe. Someone can correct me if I'm wrong or provide more specific details. Anyway, the gist being - someplace passed their CAP inspection and someone (an employee I believe) contacted another regulatory agency because there was NO way this place should've passed CAP. When this all came to light CAP had to respond, and as can often be the case, the response was overly compensatory. I'm sure a lot of folks out there know what I mean. Now CAP issues confusing and sometimes unnecessary regulation changes and additions. And of course we're all aware of the "super secret surprise" inspections. I'm not even sure of half the other hidden agendas and possible ulterior motives. Control issues, tarnished pride, bruised egos and all conveniently cloaked in a drive for the best possible patient care...who could argue with that standard? During my previous employment, we "sweated" the details and worked diligently to achieve our 1st CAP accreditation circa 2000. I have to admit, I did/do like the regulation format. Having a question asked, determining if it applies to your service, and then answering yes or no. By taking it from there and doing things on the up and up, most any lab, that's honest and conscientious should have the realistic expectation of passing CAP. That was then, it seems to longer be that way. As I mentioned, confusing language and reg. additions/changes, along with CAP inspectors and their agendas have all been to the detriment of the accreditation. I was trained to look at CAP as "peer review". In my experience, many times this was not the case. Many CAP inspecting "teams" wants to make the "inspectees" (if that's a word) something of a clone, carbon copy or version of the inspecting team's service. This is another huge problem and causes a lot of strife, hard feelings and red tape at and after the summations. The regulations, ideally, should be interpreted in the most objective way possible. Again, maybe it's human nature, but it seems that people can't help being overly subjective re: interpretations of any number of CAP regs. I used to work with a pathologist that regularly attended the CAP committee meetings. At times I would bring issues to him I thought relevant to CAP. I don't recall the specifics but I do know they were of a practical nature from a technical viewpoint. I basically got the brush-off and was led to believe that CAP wasn't interested in the "technical" viewpoint and he wasn't going to bother with it. This may be a stretch in logic on my part...however, I can't help but think if CAP would listen to technical folks as well as MDs, they'd be in a better position right now. I'm not inclined to throw the baby out with the bath water. I think CAP accrediting was established with good intentions. Somehow things have gotten out of hand, and some have gone horribly wrong. I think things like QIP are good, although I've heard complaints about that as well. I'm feeling lucky these days because CAP isn't in my life. But my attitudes and mind-set have most certainly been shaped by my CAP experiences. Please remember this is my opinion only, I am not perfect and am only interested in practical application of sensible regulations for optimal patient care. Regards, Tom Jasper Histology Supv. CORPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Thursday, April 01, 2010 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with these subjective entities? The BS I have heard over the last few months concerning MOHS surgery specimens is one glaring example of the limitations CAP has in understanding fully certain nuances of the lab trade. Ridiculous. Unless you want the marketing and potential "perception" that you are better covered from a legal standpoint, CAP certs are worthless. The more I hear about CAP certifications, the more I see it as a certain community of individuals who are protecting their perceived "TURF." In the end, the pathologists in the group and in the facility in which you are working have to take responsibility for these matters. If the docs think a CAP cert is necessary, then do it and live with it. If not, then consider yourself lucky to not have to see these people in your lab. I have been through MANY CAP inspections in and out of the military. For the most part, though, I see people paying this organization to inspect their lab as the same thing as "burning a pinch of incense in honor of great Caesar, ruler of Rome." It will get you some kudos, but tangibly not change much at all if your pathologists or HR $ hiring hands want to pocket more $ as a result of hiring "pregnant out of wedlock 16 year olds" to gross tissue and cut slides. Seen it. AB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mary.Clarke <@t> wfhc.org Thu Apr 1 14:00:43 2010 From: Mary.Clarke <@t> wfhc.org (Clarke, Mary) Date: Thu Apr 1 14:01:00 2010 Subject: [Histonet] Open position In-Reply-To: <20100331170401.470F85EC266@wfcuda02.wfhc.org> References: <20100331170401.470F85EC266@wfcuda02.wfhc.org> Message-ID: <51C4369B81AC3B42942D85DF65E1D8850DE92C77@WFEXBE04.wfsi.priv> Wheaton Franciscan Healthcare in Milwaukee, Wisconsin has an opening for a Full Time Histologist. The position is full time and working first shift. All interested parties should apply at mywheaton.org. Terri Clarke Histology/Cytology Supervisor Wheaton Franciscan Laboratories 11020 W. Plank Ct. Wauwatosa, Wi 53226 mary.clarke@wfhc.org 414-256-5564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 47 X-MS-Antispam-Report: v=1.1 cv=4P7/mf10x8dEHGH2ciP9LnGQA5lgVfqkCQv4e7nAWiY= c=1 sm=1 a=wjsViedNE1AA:10 a=ORa4HqFjfvEA:10 a=kj9zAlcOel0A:10 a=l35eeWiB97AM7zGLAHnv6Q==:17 a=rGTX7NEOAAAA:8 a=VZKIPUO1AAAA:8 a=xw0jMZ-1AAAA:8 a=AiK540Z-AAAA:8 a=3oc9M9_CAAAA:8 a=wmuHnsCxAAAA:8 a=MB2RxddlAAAA:8 a=ifpf15b9AAAA:8 a=pGLkceISAAAA:8 a=69EAbJreAAAA:8 a=DPdd6oeZAAAA:8 a=VJfHWhI1AAAA:8 a=De6jasrnAAAA:8 a=CjxXgO3LAAAA:8 a=pcLIrrrKAAAA:8 a=hG1ilDDYAAAA:8 a=Zk3OmFfbAAAA:8 a=_Yv7lGqQAAAA:8 a=mK_AVkanAAAA:8 a=JEPI03ISAAAA:8 a=dqKF6MorAAAA:8 a=4rwqQP4yAAAA:8 a=FmX3VfpdAAAA:8 a=QoHJ8kPOAAAA:8 a=LZMJPUbRAAAA:8 a=J22QVjqCAAAA:8 a=iYnMqK-LAAAA:8 a=9tgMEWK8AAAA:8 a=SreLMRJ_JLyT-dOW9VMA:9 a=8wncxCKgDhVt3pfHsNYA:7 a=yTZPsDzJpOwiGpUIJxbTeVlTr2EA:4 a=CjuIK1q_8ugA:10 a=UBFB8_TgbCAA:10 a=YBfCUMaO864A:10 a=bBdlfeTYgc8A:10 a=LCI_S3SBbtoA:10 a=hFW5seEGZPIA:10 a=3k3OjZZvaIcA:10 a=-ZqTAXztC80A:10 a=h9GhwDwmgYsA:10 a=_y9yZdQfWNQA:10 a=Rndkm7_YJgkA:10 a=gs6uBmZkH1IA:10 a=wyxuOhmUE0sA:10 a=Q9wsnxoZDP4A:10 a=eIAu5hglp_gA:10 a=7TNh3tuScToA:10 a=m3eO0MWQufIA:10 a=n0JjG0G76ZIA:10 a=SsRibNONUAYA:10 a=7BQJm5KJwvkA:10 a=egex1VcOG48A:10 a=PHtLdjekbNMA:10 a=NWuXyuRbOlcA:10 a=6UTQ9x4c_LIA:10 a=GcO_A_HHfaEA:10 a=bLDI6qII-1kA:10 a=olHdEMRXPo4A:10 a=7xT2_2TitfEA:10 a=mWA6RMptvkMA:10 a=qMSKYvDJ6ecA:10 a=Pq1fDWCDjukA:10 a=hEOfLE5TTYkA:10 a=U8Ie8EnqySEA:10 a=vT91-_uDLb8A:10 a=k_UBRwXnWxAA:10 a=wQCegDD2Sp0A:10 a=MSl-tDqOz04A:10 a=EfJqPEOeqlMA:10 a=8pQ2e33ZHLcA:10 a=ibrNy1gtLJsA:10 a=rC2wZJ5BpNYA:10 a=ftFGBYpk1mUA:10 a=7x4CYCAcUd4DwaFL:21 a=uC35AA0cyO_iCfn2:21 a=l35eeWiB97AM7zGLAHnv6Q==:117 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> (Andrew Burgeson) 2. retention of prosthetic valves (Lise Matzke) 3. Re: RE: lab markers KP (Merced M Leiker) 4. Message-ID: (Andrew Burgeson) 5. RE: retention of prosthetic valves (Weems, Joyce) ---------------------------------------------------------------------- Message: 1 Date: Wed, 31 Mar 2010 11:05:19 -0500 From: "Andrew Burgeson" Subject: [Histonet] <29A3CB81288E6F4BA2C9B3C8015A9A130176AC22@MD1EV002.medimmune.com> To: histonet@lists.utsouthwestern.edu Message-ID: <4bb372bf.2ea.32e1.1115715446@siscom.net> Content-Type: text/plain; charset="iso-8859-1" I agree that these pens are excellent. Many years ago at the National Naval Medical center in Bethesda, MD we had an entire run of cassettes marked with "Xylene-proof" markers come out of the processor with all the ink dissolved and NOTHING on the cassettes! Fortunately, we had everything in order with a grossing log and were religious about keeping the order of grossed cassettes. This was a scary deal. SO I WILL NEVER TRUST THESE FOR CASSETTES, but I DO THINK that KP markers are great and the MOHS tech at the last practice and lab i worked for uses them. KP definitely good. I don't recommend any you havent used before...or test them first. A bad batch of ink and your entire run is blank. ------------------------------ Message: 2 Date: Wed, 31 Mar 2010 09:21:52 -0700 From: "Lise Matzke" Subject: [Histonet] retention of prosthetic valves To: Message-ID: <4BB3142F.F029.0016.1@hli.ubc.ca> Content-Type: text/plain; charset=US-ASCII Hi there, I was wondering what your hospital/centre's pathology policies indicate in terms of the time you retain prosthetic valve specimens? Months? Years? thanks for your feedback, Anne Marie ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ------------------------------ Message: 3 Date: Wed, 31 Mar 2010 12:40:55 -0400 From: Merced M Leiker Subject: Re: [Histonet] RE: lab markers KP To: "Blazek, Linda" , "'Madary, Joseph'" , histonet@lists.utsouthwestern.edu Message-ID: <128EF1E2E83A8ED95DFA6416@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed I agree as well! - I use them to mark everything, even things that don't get treated with chemicals. One note: they work best if you mark hard non-porous surfaces (like the plastic cassettes) the day before to give the ink time to bind with the material. Regards, Merced --On Wednesday, March 31, 2010 11:31 AM -0400 "Blazek, Linda" wrote: > I have to agree. They have never failed and work forever. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, > Joseph Sent: Wednesday, March 31, 2010 11:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] lab markers KP > > The only consistent lab pen I have used that will hold up under decal, > processing with various alcohol, xylene, hemo de is the KP marker plus. > I am sure there are several vendors, I use Mercedes. > > Nick Madary, HT/HTL(ASCP)QIHC > Medimmune Histology Mgr, > OMW, Area 4, Lab 2438 > 301.398.4745(vm) > 301.398.6360(lab) > 301.398.9745(fax) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010 > 10:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 76, Issue 45 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Coverslipping with SubX (Vanessa Avalos) > 2. Haunted cryostat/Thanks! (mtitford@aol.com) > 3. GMS on decal tissue (Morken, Tim) > 4. questions (Webb, Dorothy L) > 5. Re: her2 validation (Pat Laurie) > 6. FREE MONEY! (Jackie M O'Connor) > 7. RE: questions (Garrison, Becky) > 8. Thermo's Excelsior Tissue Processor Program's > (Akemi Allison-Tacha) > 9. re: cyto prep tech (Kim Tournear) > 10. her-2 neu validation (Debbie Nannenga) > 11. RE: questions (Tony Henwood) > 12. RE: RE: Leica Paraplast (connie grubaugh) > 13. (no subject) (Green JumpyOne) > 14. Fwd: [Histonet] (no subject) (Malika Benatti) > 15. RE: Thermo's Excelsior Tissue Processor Program's > (Heckford, Karen - SMMC-SF) > 16. RE: RE: Leica Paraplast (Nails, Felton) > 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett) > 18. Texas Society for Histotechnology April 23-25, 2010 > (kdwyer3322@aol.com) > 19. cassette labels erased by processor (Catherine Breen) > 20. RE: her2 validation (Weems, Joyce) > 21. RE: cassette labels erased by processor (Sherwood, Margaret ) > 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller) > 23. Re: cassette labels erased by processor (Malika Benatti) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 30 Mar 2010 11:15:38 -0700 > From: "Vanessa Avalos" > Subject: [Histonet] Coverslipping with SubX > To: "'HISTONET LISTS'" > Message-ID: <000001cad035$00d2f5b0$0278e110$@com> > Content-Type: text/plain; charset="us-ascii" > > Is anyone using SubX ? I am trying it out and am having some difficulty > coverslipping. I am using the Subx glue as directed since Acrymount didn't > seem to work as well. I still get a hazy film under the glass and am > getting big air bubbles as well. I can eventually get them out but its > just takes a while and you know how time is precious when you have a line > of slides to stain. The process is not as smooth as before. I really > would like this to work out for me and eliminate xylene. > > Any suggestions?? > > > > Vanessa > > Fax: 602-277-2134 > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 30 Mar 2010 14:44:26 -0400 > From: mtitford@aol.com > Subject: [Histonet] Haunted cryostat/Thanks! > To: Histonet@pathology.swmed.edu > Message-ID: <8CC9E5028ADFB36-1500-2BE5@webmail-m010.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > > Thank you everyone who responded to my problem with a Leica CM 1850 > cryostat turning itself off. About 11 people responded with tips. Thank > you!! The Histonet is great!! In answer to some enquirys: > 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts > for an hour. Jackie O' Conner asks if it is set to go back "on" after the > defrost. I have no idea. It defrosts well the rest of the time, and turns > itself back on. Brian Cornett-Early recommends changing the start device > on the compressor. 2) Someone else asked if power is getting to the > cryostat - Yes, when it turns itself off, the on/off switch is on "off". > All you have to do is flip the switch and it starts pumping and cooling. > 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on > the side of the compressor. That is probably where we will start. Mari > Ann works for Leica so it sounds like good advice. > > Thank you everyone. Since it only breaks down about once a month, it will > take a long time to determine if the problem is fixed. > > Michael Titford > Pathology USA > Mobile AL USA > > > > ------------------------------ > > Message: 3 > Date: Tue, 30 Mar 2010 11:52:17 -0700 > From: "Morken, Tim" > Subject: [Histonet] GMS on decal tissue > To: histonet > Message-ID: > > <1AAF670737F193429070841C6B2ADD4C013B16C780@EXMBMCB15.ucsfmedicalcenter. o > rg> > > Content-Type: text/plain; charset=us-ascii > > Has anyone seen or heard of problems with Grocott Methenamine Silver > staining for fungi on decal tissues? Specifically, background or false > positives? I can't find anything in any books that gives any indications > to not use decaled tissue. > > Thanks! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > Box 1656 > 1600 Divisadero St. > San Francisco, CA 94143-1656 > USA > > Phone: (415) 514-6042 > Pager: (415) 443-6509 > Fax: (415) 885-7409 > > Email: tim.morken@ucsfmedctr.org > > > > ------------------------------ > > Message: 4 > Date: Tue, 30 Mar 2010 14:03:55 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] questions > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846@HPEMX3.HealthPartners.int> > Content-Type: text/plain; charset="us-ascii" > > We have been having problems with underprocessed placental membrane, some > of which are cut fairly thick, but am seeing it on more than just the > thich samples. Does anyone out there in histoland have a special process > for placentas or any helpful hints? I do know that many times the > placentas sit without formalin in L&D for hours before they bring them to > histo and add formalin and this seems to me it could be a factor, even > though we have them sitting in formalin for a few hours before processing. > > Also, does anyone do the high-iron diamine stain for intestinal mucin > staining? Do you do it with an Alcian Blue-PAS stain? > > Thank you ahead of time for any and all responses!! > > Dorothy Webb, HT (ASCP) > Regions Histology Lab > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > > > ------------------------------ > > Message: 5 > Date: Tue, 30 Mar 2010 13:26:21 -0700 > From: Pat Laurie > Subject: Re: [Histonet] her2 validation > To: anita dudley > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > We left it up to our pathologist. Initially we did the 25 slide > validation, our pathologist wasn't 100% convinced that he could read them > accurately, so we did another 25, plus all of the ones we sent out which > already had FISH ran. It ended up being almost 70 cases, and our > pathologist was properly "educated" on how he should score them. He felt > that there was excessive pressure put on the pathologists due to having > to score these slides, not just say negative or positive. I'll say > though for the last year or so, with about 6 her2 cases ran a day, he has > been remarkably accurate. Any that he scores 2+ and has sent out for > FISH he usually indicates if he thinks that it will be positive or > negative. He has yet to be wrong. Another Pathologist who I talked to > who helped set up the guidelines said that there isn't a "1 method fits > all" process. Good luck. > > On Tue, Mar 30, 2010 at 7:19 AM, anita dudley > wrote: > >> >> sorry!!! I didn't put a subject in the previous post. wondering about >> how many slides people are using for her2 validation. thanks >> >> >> >> anita dudley >> >> providence hospital >> >> mobile alabama >> >> _________________________________________________________________ >> The New Busy is not the old busy. Search, chat and e-mail from your >> inbox. >> >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: >> ON:WL:en-US:WM_HMP:032010_3_____________________________________________ >> __ Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > PH: 206-215-5949 > plaurie@cellnetix.com > > > ------------------------------ > > Message: 6 > Date: Tue, 30 Mar 2010 15:28:30 -0500 > From: Jackie M O'Connor > Subject: [Histonet] FREE MONEY! > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Now that I have your attention - - > > I would really like to hear from you if you are at all interested in a > histology position at Abbott Laboratories in Northern Illinois. Even if > you have applied previously. > > This is a great opportunity to work in a GLP research lab, a good deal of > bench work, but developing IHC for toxicology purposes as well. We have > a Biocare intelliPATH stainer - it's FUN! > > We have four full time technicians/technologists with a fifth open > position. Abbott offers 3 weeks paid vacation, great benefits, and is a > good company to work for. I've been here ten years, and I've never been > anywhere 10 years. > > Please forward your resume to me, as well as go to www.Abbott.com to > apply. > > Jackie O'Connor > > > ------------------------------ > > Message: 7 > Date: Tue, 30 Mar 2010 16:49:42 -0400 > From: "Garrison, Becky" > Subject: RE: [Histonet] questions > To: "Webb, Dorothy L" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We receive the placentas fresh (they are refrigerated in L&D before > transport to Pathology); add lots of formalin (use 163 oz containers) > and let fix overnight. Early next morning, placenta is grossed and > cassettes sit in formalin til end of day. This formalin is changed at > least once so that bloody formalin is replaced with fresh. Placed on > processor at end of second day after receipt. > > When we had L&D add formalin, there was never enough formalin added and > the placentas sat unfixed at room temperature for long periods of time. > This procedure works better for us. Yes, we can not meet the CAP > guideline > for 2 day TAT but do end up with a consistently better quality product > for this tissue type. (Placentas make up a small portion of overall > workload, so overall TAT is not affected). Prior to this procedure, > placentas made > up a disproportionate amount of reprocessed blocks. > > Becky Garrison > Pathology Supervisor > Shands Jacksonville > Jacksonville, FL 32209 > 904-244-6237, phone > 904-244-4290, fax > 904-393-3194, pager > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Tuesday, March 30, 2010 3:04 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] questions > > We have been having problems with underprocessed placental membrane, > some of which are cut fairly thick, but am seeing it on more than just > the thich samples. Does anyone out there in histoland have a special > process for placentas or any helpful hints? I do know that many times > the placentas sit without formalin in L&D for hours before they bring > them to histo and add formalin and this seems to me it could be a > factor, even though we have them sitting in formalin for a few hours > before processing. > > Also, does anyone do the high-iron diamine stain for intestinal mucin > staining? Do you do it with an Alcian Blue-PAS stain? > > Thank you ahead of time for any and all responses!! > > Dorothy Webb, HT (ASCP) > Regions Histology Lab > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will > be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT) > From: Akemi Allison-Tacha > Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's > To: histo net > Message-ID: <465571.85431.qm@web113812.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi All of you in Histoland, > > I am working with a facility that recently purchased a Thermo Excelsior > Tissue Processor. They have had processing problems with several of > their specimens using non-xylene clearing agent. These issues were with > both bx's and larger tissues. The program and the non-xylene clearing > agent was recommended by the technical staff at Thermo. > > The histology staff have switched back to using xylene verses the > non-xylene clearing agent, and most of the issues have disappeared. > > Also, the histotech's were using reagent alcohol, histological grade. > This grade of alcohol is denatured with methyl alcohol. The sales > representative was in and informed us that this type of alcohol has > damaging effects on the instrument. The representative will be coming in > to flush out the system and reprogram the instrument next Monday. > > I looked over the current VIP program which is being used, and the > program, timing and temperatures are a little different from most > hospital and private laboratories I have worked with. We are going to > use basically the same program for the Excelsior, that we use on the VIP. > > I would like to know what other Excelsior Tissue Processor users that use > xylene have for their programs. It would be great to compare the > reagents, programs, timing, and temperatures for Routine overnight runs > and for Rapid Biopsy Runs. Thank you in advance for your assistance. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > > ------------------------------ > > Message: 9 > Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT) > From: Kim Tournear > Subject: [Histonet] re: cyto prep tech > To: Histonet > Message-ID: <839809.46245.qm@web54204.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Thank you to everyone who responded to my question about cyto prep tech > work, > > > > ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) > Histology Supervisor > Tucson Medical Center > Tucson, AZ > ~Don't let your life end before it begins~ > OU Rocks!!!! > > > > > ------------------------------ > > Message: 10 > Date: Tue, 30 Mar 2010 17:12:57 -0700 > From: Debbie Nannenga > Subject: [Histonet] her-2 neu validation > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Anita, > > > > > > We ran 25 amplified and 25 negative cases for our validation study. > > > > Good Luck. > > > > Debbie Nannenga, HTL(ASCP) QIHC > > InCyte Pathology > > Spokane, WA > > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 31 Mar 2010 11:21:58 +1100 > From: "Tony Henwood" > Subject: RE: [Histonet] questions > To: "Webb, Dorothy L" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Dorothy, > > Apart from hoping the blocks of tissue are not too thick, we microwave > the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This > ensures adequate fixation prior to processing on a Shandon Excelsior > Tissue Processor. > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Wednesday, 31 March 2010 6:04 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] questions > > > We have been having problems with underprocessed placental membrane, > some of which are cut fairly thick, but am seeing it on more than just > the thich samples. Does anyone out there in histoland have a special > process for placentas or any helpful hints? I do know that many times > the placentas sit without formalin in L&D for hours before they bring > them to histo and add formalin and this seems to me it could be a > factor, even though we have them sitting in formalin for a few hours > before processing. > > Also, does anyone do the high-iron diamine stain for intestinal mucin > staining? Do you do it with an Alcian Blue-PAS stain? > > Thank you ahead of time for any and all responses!! > > Dorothy Webb, HT (ASCP) > Regions Histology Lab > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will > be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************************************************************************ * > ******** This email and any files transmitted with it are confidential > and intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please delete it > and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned > and although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ************************************************************************ * > ******** > > > > ------------------------------ > > Message: 12 > Date: Tue, 30 Mar 2010 19:58:21 -0700 > From: connie grubaugh > Subject: RE: [Histonet] RE: Leica Paraplast > To: , > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > Hi all, I questioned the Leica paraplast that we have received too. It > is real gummy and sticky. Takes me forever to cut. I asked and was > informed that it is the same stuff we have always got and there is no > difference. Except all of us techs have noticed a big difference. > > > > Connie G. > > > > > >> Date: Tue, 23 Mar 2010 11:29:03 -0700 >> From: ddreesen@sbcglobal.net >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: Leica Paraplast >> >> >> Hi Kristen, >> We were using the Paraplast Xtra and switched to something else after we >> noticed a difference in the quality of the paraffin. The tissues weren't >> cutting as well and the paraffin seemed to be "gritty". We found we were >> going through many more blades due to nicks and scratches and many times >> had to switch blades mid-block. >> >> >> From: histonet-request@lists.utsouthwestern.edu >> > Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) >> From: kristen arvidson >> Subject: [Histonet] Leica Paraplast >> To: histonet >> Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Has anyone who uses paraplast (we use the basic one) noticed a change in >> the quality of your tissue? I have recently found out that they have >> changed manufacturing sites in the past couple of months. I am having >> on and off issues with my skin specimens that have been going on for >> about 2 months or so. Thought there may be a correlation. Any thoughts? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail: Trusted email with Microsoft's powerful SPAM protection. > http://clk.atdmt.com/GBL/go/210850552/direct/01/ > > ------------------------------ > > Message: 13 > Date: Wed, 31 Mar 2010 01:52:42 -0700 > From: Green JumpyOne > Subject: [Histonet] (no subject) > To: , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > http://www.trainedlabor.com/H6UH4Yh1UJ.html > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: O > N:WL:en-US:WM_HMP:032010_3 > > ------------------------------ > > Message: 14 > Date: Wed, 31 Mar 2010 10:45:09 +0100 > From: Malika Benatti > Subject: Fwd: [Histonet] (no subject) > To: Histonet List > Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6@gmail.com> > Content-Type: text/plain; charset=us-ascii; format=flowed; > delsp=yes > > Dear Histonet list manager > > Is they anyway you could filter/block spam such as this one from be > sent out to the Histonet list. > > Cheers > > Malika Benatti BSc MIBMS > Specialist Biomedical Scientist > Great Ormond Street Children Hospital > London > > " ... Smile it confuses people ..." > > Begin forwarded message: > >> From: Green JumpyOne >> Date: 31 March 2010 09:52:42 GMT+01:00 >> To: , , >> > > >> Subject: [Histonet] (no subject) >> > >> http://www.trainedlabor.com/H6UH4Yh1UJ.html >> >> _________________________________________________________________ >> The New Busy is not the old busy. Search, chat and e-mail from your >> inbox. >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL: >> ON:WL:en-US:WM_HMP:032010_3_____________________________________________ >> __ Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 15 > Date: Wed, 31 Mar 2010 05:02:26 -0700 > From: "Heckford, Karen - SMMC-SF" > Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's > To: "Akemi Allison-Tacha" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <2842DC75AE43AA4B92954CFB31781BC105697F76@CHW-MSG-301.chw.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Pretty much any problems I have had with the Excelsior and I have been > using one for 4 years now is with Pathologists loading it incorrectly. I > use reagent alcohol in it all the time, with no problems. I prefer > using xylene in my processor. Every single time I have switched and > started using a non-xylene sub. I have had nothing but problems. If I > use a non-xylene I save it for the stainer. Take it easy, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM > To: histo net > Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's > > Hi All of you in Histoland, > > I am working with a facility that recently purchased a Thermo Excelsior > Tissue Processor. They have had processing problems with several of > their specimens using non-xylene clearing agent. These issues were with > both bx's and larger tissues. The program and the non-xylene clearing > agent was recommended by the technical staff at Thermo. > > The histology staff have switched back to using xylene verses the > non-xylene clearing agent, and most of the issues have disappeared. > > Also, the histotech's were using reagent alcohol, histological grade. > This grade of alcohol is denatured with methyl alcohol. The sales > representative was in and informed us that this type of alcohol has > damaging effects on the instrument. The representative will be coming in > to flush out the system and reprogram the instrument next Monday. > > I looked over the current VIP program which is being used, and the > program, timing and temperatures are a little different from most > hospital and private laboratories I have worked with. We are going to > use basically the same program for the Excelsior, that we use on the VIP. > > I would like to know what other Excelsior Tissue Processor users that use > xylene have for their programs. It would be great to compare the > reagents, programs, timing, and temperatures for Routine overnight runs > and for Rapid Biopsy Runs. Thank you in advance for your assistance. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Wed, 31 Mar 2010 07:27:34 -0500 > From: "Nails, Felton" > Subject: RE: [Histonet] RE: Leica Paraplast > To: "'connie grubaugh'" , > "ddreesen@sbcglobal.net" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > tal.org> > > Content-Type: text/plain; charset=us-ascii > > There is about three brands of paraplast and they all cut differently. In > one of my labs I use paraplast plus and it ribbons very well however the > blocks have to be colder then if you are using TissuePrep from Fisher. > Leica may have sent you one of the other types of paraplast. (paraplast, > Paraplast Plus, Paraplast Xtra) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie > grubaugh Sent: Tuesday, March 30, 2010 9:58 PM > To: ddreesen@sbcglobal.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Leica Paraplast > > > Hi all, I questioned the Leica paraplast that we have received too. It > is real gummy and sticky. Takes me forever to cut. I asked and was > informed that it is the same stuff we have always got and there is no > difference. Except all of us techs have noticed a big difference. > > > > Connie G. > > > > > >> Date: Tue, 23 Mar 2010 11:29:03 -0700 >> From: ddreesen@sbcglobal.net >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: Leica Paraplast >> >> >> Hi Kristen, >> We were using the Paraplast Xtra and switched to something else after we >> noticed a difference in the quality of the paraffin. The tissues weren't >> cutting as well and the paraffin seemed to be "gritty". We found we were >> going through many more blades due to nicks and scratches and many times >> had to switch blades mid-block. >> >> >> From: histonet-request@lists.utsouthwestern.edu >> > Message: 4 >> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT) >> From: kristen arvidson >> Subject: [Histonet] Leica Paraplast >> To: histonet >> Message-ID: <94596.93077.qm@web65707.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Has anyone who uses paraplast (we use the basic one) noticed a change in >> the quality of your tissue? I have recently found out that they have >> changed manufacturing sites in the past couple of months. I am having >> on and off issues with my skin specimens that have been going on for >> about 2 months or so. Thought there may be a correlation. Any thoughts? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail: Trusted email with Microsoft's powerful SPAM protection. > http://clk.atdmt.com/GBL/go/210850552/direct/01/________________________ _ > ______________________ Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > > > ------------------------------ > > Message: 17 > Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT) > From: Ann Bennett > Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs > To: histonet@lists.utsouthwestern.edu > Message-ID: <106398.85301.qm@web114318.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > At one point we looked at getting an Excelsior and I spoke with our sales > rep and he said all the reagents we use in our processor now are > absolutely fine. He mentioned nothing about not being able to use > Reagent alcohols or xylene substitutes. Hope this helps - have a happy > histo day! > > > > > > > ------------------------------ > > Message: 18 > Date: Wed, 31 Mar 2010 08:42:06 -0400 > From: kdwyer3322@aol.com > Subject: [Histonet] Texas Society for Histotechnology April 23-25, > 2010 > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CC9EE6B49D9799-17C0-9D76@webmail-m051.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > Histonetters, > It is not too late to join us for the 2010 TSH meeting in Houston Texas > April 23-25, 2010. > > The fun starts Thursday April 22, 2010 with a Golf outing open to all > Vendors and Attendees. > > Workshops begin Friday April 23, 2010 at 8:00am. > > There is still plenty of time to register and get a hotel room to enjoy 2 > full days of workshops and symposiums. > > If you would like a program go to txsh.org or contact me via this e-mail. > > Thanks, > TSH Convention Committee > > > > > > ------------------------------ > > Message: 19 > Date: Wed, 31 Mar 2010 10:13:25 -0400 > From: "Catherine Breen" > Subject: [Histonet] cassette labels erased by processor > To: histonet@lists.utsouthwestern.edu > Message-ID: <20100331101325.1573@web007.roc2.bluetie.com> > Content-Type: text/plain; charset=UTF-8 > > I am looking for help solving a lab mystery. The cassettes in our lab > are labeled with an SP Securline Marker II and then processed in a Sakura > VIP processor. Two weeks ago the entire batch came out labeled much more > lightly than usual, some to the point of where the label was completely > effaced. Our current theory is that an acid cleanser (Citronox) or > possibly another acid was accidentally introduced into the processor. Has > any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQui Z > mGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > > ------------------------------ > > Message: 20 > Date: Wed, 31 Mar 2010 10:28:42 -0400 > From: "Weems, Joyce" > Subject: [Histonet] RE: her2 validation > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9@CHEXCMS10.one.ads.che.org> > Content-Type: text/plain; charset="us-ascii" > > >> From June-15, 2009 CAP checklist > > > > ANP.22997 Phase I N/A YES NO > > If the laboratory performs HER2 testing (HER2 protein over-expression by > immunohistochemistry [IHC] or HER2 gene amplification by in situ > hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory > documented appropriate validation for the assay(s)? > > NOTE: Initial test validation must be performed on a minimum of 25 cases > (recommended 25-100). Validation may be performed by comparing the > results of testing with a validated alternative method (i.e. IHC vs. > FISH) either in the same laboratory or another laboratory, or with the > same validated method performed in another laboratory; validation testing > must be done using the same set of cases in both labs. > > If specimens are fixed in a medium other than 10% neutral buffered > formalin, the validation study must show that results are concordant with > results from formalin-fixed tissues. > > If significant changes are made in testing methods (e.g., antibody > clone, antigen retrieval protocol or detection system, FISH probe or > pretreatment protocol), revalidation is required. > > This checklist item applies to laboratories that perform the technical > testing of specimens for HER2 amplification. Patient specimens should be > fixed in the same manner as the specimens used for the validation > study(ies). > > *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced > in-situ hybridization > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > > > ------------------------------ > > Message: 21 > Date: Wed, 31 Mar 2010 10:33:35 -0400 > From: "Sherwood, Margaret " > Subject: RE: [Histonet] cassette labels erased by processor > To: "Catherine Breen" , > > Message-ID: > <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A@PHSXMB30.partners.org> > Content-Type: text/plain; charset="us-ascii" > > I had similar issues with all of the marking pens out there. We finally > switched to Tissue-Tek Marking pencils #4160 and have not had a problem > since. Plus they never "dry" out! > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine > Breen Sent: Wednesday, March 31, 2010 10:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette labels erased by processor > > I am looking for help solving a lab mystery. The cassettes in our lab are > labeled with an SP Securline Marker II and then processed in a Sakura VIP > processor. Two weeks ago the entire batch came out labeled much more > lightly than usual, some to the point of where the label was completely > effaced. Our current theory is that an acid cleanser (Citronox) or > possibly another acid was accidentally introduced into the processor. > Has any lab experienced this problem before, especially with Citronox? > > Thank you. > > ------------------------------------------------------------ > Best Weight Loss Program - Click Here! > Weight Loss Program > http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQui Z > mGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it > is addressed. If you believe this e-mail was sent to you in error and the > e-mail contains patient information, please contact the Partners > Compliance HelpLine at http://www.partners.org/complianceline . If the > e-mail was sent to you in error but does not contain patient information, > please contact the sender and properly dispose of the e-mail. > > > > > ------------------------------ > > Message: 22 > Date: Wed, 31 Mar 2010 09:39:46 -0500 > From: Cheri Miller > Subject: FW: [Histonet] cassette labels erased by processor > To: "histonet-bounces@lists.utsouthwestern.edu" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > > > YES! That is why I use pencil only. I had a processor full of blank/ > unlabeled cassettes because the lot# of the pens was bad. There is no way > of knowing if the ink is reagent proof until a disaster like you have > occurs. You don't always know if the ink lot passed its QC with absolute > certainty. Pencil is fail proof. I hope your day gets better, Cheri > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine > Breen Sent: Wednesday, March 31, 2010 9:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette labels erased by processor > > I > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible > for delivering it to the addressee, you are hereby notified that you are > in possession of confidential and privileged information. Any > dissemination, distribution, or copying of this e-mail is strictly > prohibited. If you have received this message in error, please notify > the sender immediately and delete this email from your system. > > > > ------------------------------ > > Message: 23 > Date: Wed, 31 Mar 2010 15:40:44 +0100 > From: Malika Benatti > Subject: Re: [Histonet] cassette labels erased by processor > To: Catherine Breen > Cc: Histonet List > Message-ID: > Content-Type: text/plain; charset=us-ascii; format=flowed; > delsp=yes > > Hi there, > > I would suggest to use a pencil rather than a marker pen to label > cassettes when processing cassette with the Sakura VIP , my lab had a > number of the so called solvent proof pen on trial and have yet to > find one that survive processing. > > > > > Malika Benatti BSc MIBMS > Specialist Biomedical Scientist > Great Ormond Street Children Hospital > London > > " ... Smile it confuses people ..." > > On 31 Mar 2010, at 15:13, "Catherine Breen" wrote: > >> I am looking for help solving a lab mystery. The cassettes in our >> lab are labeled with an SP Securline Marker II and then processed in >> a Sakura VIP processor. Two weeks ago the entire batch came out >> labeled much more lightly than usual, some to the point of where the >> label was completely effaced. >> Our current theory is that an acid cleanser (Citronox) or possibly >> another acid was accidentally introduced into the processor. >> Has any lab experienced this problem before, especially with Citronox? >> >> Thank you. >> >> ------------------------------------------------------------ >> Best Weight Loss Program - Click Here! >> Weight Loss Program >> http://tagline.excite.com/c? >> cp= >> etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADN >> AAAAAAAAAAAAAAAAAAAEUlAqCWY= >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 76, Issue 45 > **************************************** > > > > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information is > considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the > individual(s) for whom it is intended. If you have received this > electronic communication in error, please reply to the sender advising of > the error in transmission and delete the original message and any > accompanying documents from your system immediately, without copying, > reviewing or otherwise using them for any purpose. Thank you for your > cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 4 Date: Wed, 31 Mar 2010 11:51:47 -0500 From: "Andrew Burgeson" Subject: [Histonet] Message-ID: To: histonet@lists.utsouthwestern.edu Message-ID: <4bb37da3.3e6.4886.355847169@siscom.net> Content-Type: text/plain; charset="iso-8859-1" I use nothing but SurgiPath (now LEICA) Blue Ribbon Paraffin. I run dermpath labs and this stuff is formulated for optimal skin sectioning. Highly recommend it....havent used anything else for 13 years. AB ------------------------------ Message: 5 Date: Wed, 31 Mar 2010 12:55:40 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] retention of prosthetic valves To: Lise Matzke , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16405E029F8@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We photograph and retain only if requested. If not requested, we discard with the regular specimens usually at least a month. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lise Matzke Sent: Wednesday, March 31, 2010 12:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] retention of prosthetic valves Hi there, I was wondering what your hospital/centre's pathology policies indicate in terms of the time you retain prosthetic valve specimens? Months? Years? thanks for your feedback, Anne Marie ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 47 **************************************** Privileged/Confidential information may be contained in this message. The information contained in this message is intended only for the use of the recipient(s) named above and their co-workers who are working on the same matter. The recipient of this information is prohibited from disclosing the information to any other party unless this disclosure has been authorized in advance. If you are not intended recipient of this message or any agent responsible for delivery of the message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of this message is strictly prohibited. You should immediately destroy this message and kindly notify the sender by reply E-Mail. Please advise immediately if you or your employer does not consent to Internet E-Mail for messages of this kind. Opinions, conclusions and other information in this message that do not relate to the official business of the firm shall be understood as neither given nor endorsed by it. From liz <@t> premierlab.com Thu Apr 1 14:47:15 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Apr 1 14:47:20 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: <90354A475B420441B2A0396E5008D49692BF2E@copc-sbs.COPC.local> Message-ID: Thomas Just a question, what would you consider an unnecessary CAP regulation? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, April 01, 2010 12:46 PM To: Andrew Burgeson Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com I'm inclined to agree with you Andrew. Seems to me that CAP has become, unintentionally (I'd like to believe) something of an unsavory, bullying sort of entity. I'm not certain about all the factors involved, but I think a few things have definitely contributed to all of this CAP negativity. First of all - CAP was (is?) considered the accrediting "gold standard". That's pretty heady stuff...possible ego inflation potential...high horse attitude...elitism? Not saying that was the plan, just an unfortunate and unintended consequence. I'm sensing a power trip here, it's partly human nature (I guess) and it can definitely suck! Secondly - as I recall, CAP got egg on their face a few years back in the DC/Baltimore area I believe. Someone can correct me if I'm wrong or provide more specific details. Anyway, the gist being - someplace passed their CAP inspection and someone (an employee I believe) contacted another regulatory agency because there was NO way this place should've passed CAP. When this all came to light CAP had to respond, and as can often be the case, the response was overly compensatory. I'm sure a lot of folks out there know what I mean. Now CAP issues confusing and sometimes unnecessary regulation changes and additions. And of course we're all aware of the "super secret surprise" inspections. I'm not even sure of half the other hidden agendas and possible ulterior motives. Control issues, tarnished pride, bruised egos and all conveniently cloaked in a drive for the best possible patient care...who could argue with that standard? During my previous employment, we "sweated" the details and worked diligently to achieve our 1st CAP accreditation circa 2000. I have to admit, I did/do like the regulation format. Having a question asked, determining if it applies to your service, and then answering yes or no. By taking it from there and doing things on the up and up, most any lab, that's honest and conscientious should have the realistic expectation of passing CAP. That was then, it seems to longer be that way. As I mentioned, confusing language and reg. additions/changes, along with CAP inspectors and their agendas have all been to the detriment of the accreditation. I was trained to look at CAP as "peer review". In my experience, many times this was not the case. Many CAP inspecting "teams" wants to make the "inspectees" (if that's a word) something of a clone, carbon copy or version of the inspecting team's service. This is another huge problem and causes a lot of strife, hard feelings and red tape at and after the summations. The regulations, ideally, should be interpreted in the most objective way possible. Again, maybe it's human nature, but it seems that people can't help being overly subjective re: interpretations of any number of CAP regs. I used to work with a pathologist that regularly attended the CAP committee meetings. At times I would bring issues to him I thought relevant to CAP. I don't recall the specifics but I do know they were of a practical nature from a technical viewpoint. I basically got the brush-off and was led to believe that CAP wasn't interested in the "technical" viewpoint and he wasn't going to bother with it. This may be a stretch in logic on my part...however, I can't help but think if CAP would listen to technical folks as well as MDs, they'd be in a better position right now. I'm not inclined to throw the baby out with the bath water. I think CAP accrediting was established with good intentions. Somehow things have gotten out of hand, and some have gone horribly wrong. I think things like QIP are good, although I've heard complaints about that as well. I'm feeling lucky these days because CAP isn't in my life. But my attitudes and mind-set have most certainly been shaped by my CAP experiences. Please remember this is my opinion only, I am not perfect and am only interested in practical application of sensible regulations for optimal patient care. Regards, Tom Jasper Histology Supv. CORPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Thursday, April 01, 2010 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with these subjective entities? The BS I have heard over the last few months concerning MOHS surgery specimens is one glaring example of the limitations CAP has in understanding fully certain nuances of the lab trade. Ridiculous. Unless you want the marketing and potential "perception" that you are better covered from a legal standpoint, CAP certs are worthless. The more I hear about CAP certifications, the more I see it as a certain community of individuals who are protecting their perceived "TURF." In the end, the pathologists in the group and in the facility in which you are working have to take responsibility for these matters. If the docs think a CAP cert is necessary, then do it and live with it. If not, then consider yourself lucky to not have to see these people in your lab. I have been through MANY CAP inspections in and out of the military. For the most part, though, I see people paying this organization to inspect their lab as the same thing as "burning a pinch of incense in honor of great Caesar, ruler of Rome." It will get you some kudos, but tangibly not change much at all if your pathologists or HR $ hiring hands want to pocket more $ as a result of hiring "pregnant out of wedlock 16 year olds" to gross tissue and cut slides. Seen it. AB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Apr 1 15:11:21 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 1 15:11:36 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: Message-ID: <74BE3D8FE1504B31861C730C0C428A8A@lurie.northwestern.edu> How about all of them? Being as it is April 1. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, April 01, 2010 2:47 PM To: Thomas Jasper; Andrew Burgeson Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Thomas Just a question, what would you consider an unnecessary CAP regulation? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, April 01, 2010 12:46 PM To: Andrew Burgeson Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com I'm inclined to agree with you Andrew. Seems to me that CAP has become, unintentionally (I'd like to believe) something of an unsavory, bullying sort of entity. I'm not certain about all the factors involved, but I think a few things have definitely contributed to all of this CAP negativity. First of all - CAP was (is?) considered the accrediting "gold standard". That's pretty heady stuff...possible ego inflation potential...high horse attitude...elitism? Not saying that was the plan, just an unfortunate and unintended consequence. I'm sensing a power trip here, it's partly human nature (I guess) and it can definitely suck! Secondly - as I recall, CAP got egg on their face a few years back in the DC/Baltimore area I believe. Someone can correct me if I'm wrong or provide more specific details. Anyway, the gist being - someplace passed their CAP inspection and someone (an employee I believe) contacted another regulatory agency because there was NO way this place should've passed CAP. When this all came to light CAP had to respond, and as can often be the case, the response was overly compensatory. I'm sure a lot of folks out there know what I mean. Now CAP issues confusing and sometimes unnecessary regulation changes and additions. And of course we're all aware of the "super secret surprise" inspections. I'm not even sure of half the other hidden agendas and possible ulterior motives. Control issues, tarnished pride, bruised egos and all conveniently cloaked in a drive for the best possible patient care...who could argue with that standard? During my previous employment, we "sweated" the details and worked diligently to achieve our 1st CAP accreditation circa 2000. I have to admit, I did/do like the regulation format. Having a question asked, determining if it applies to your service, and then answering yes or no. By taking it from there and doing things on the up and up, most any lab, that's honest and conscientious should have the realistic expectation of passing CAP. That was then, it seems to longer be that way. As I mentioned, confusing language and reg. additions/changes, along with CAP inspectors and their agendas have all been to the detriment of the accreditation. I was trained to look at CAP as "peer review". In my experience, many times this was not the case. Many CAP inspecting "teams" wants to make the "inspectees" (if that's a word) something of a clone, carbon copy or version of the inspecting team's service. This is another huge problem and causes a lot of strife, hard feelings and red tape at and after the summations. The regulations, ideally, should be interpreted in the most objective way possible. Again, maybe it's human nature, but it seems that people can't help being overly subjective re: interpretations of any number of CAP regs. I used to work with a pathologist that regularly attended the CAP committee meetings. At times I would bring issues to him I thought relevant to CAP. I don't recall the specifics but I do know they were of a practical nature from a technical viewpoint. I basically got the brush-off and was led to believe that CAP wasn't interested in the "technical" viewpoint and he wasn't going to bother with it. This may be a stretch in logic on my part...however, I can't help but think if CAP would listen to technical folks as well as MDs, they'd be in a better position right now. I'm not inclined to throw the baby out with the bath water. I think CAP accrediting was established with good intentions. Somehow things have gotten out of hand, and some have gone horribly wrong. I think things like QIP are good, although I've heard complaints about that as well. I'm feeling lucky these days because CAP isn't in my life. But my attitudes and mind-set have most certainly been shaped by my CAP experiences. Please remember this is my opinion only, I am not perfect and am only interested in practical application of sensible regulations for optimal patient care. Regards, Tom Jasper Histology Supv. CORPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Thursday, April 01, 2010 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with these subjective entities? The BS I have heard over the last few months concerning MOHS surgery specimens is one glaring example of the limitations CAP has in understanding fully certain nuances of the lab trade. Ridiculous. Unless you want the marketing and potential "perception" that you are better covered from a legal standpoint, CAP certs are worthless. The more I hear about CAP certifications, the more I see it as a certain community of individuals who are protecting their perceived "TURF." In the end, the pathologists in the group and in the facility in which you are working have to take responsibility for these matters. If the docs think a CAP cert is necessary, then do it and live with it. If not, then consider yourself lucky to not have to see these people in your lab. I have been through MANY CAP inspections in and out of the military. For the most part, though, I see people paying this organization to inspect their lab as the same thing as "burning a pinch of incense in honor of great Caesar, ruler of Rome." It will get you some kudos, but tangibly not change much at all if your pathologists or HR $ hiring hands want to pocket more $ as a result of hiring "pregnant out of wedlock 16 year olds" to gross tissue and cut slides. Seen it. AB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Thu Apr 1 14:20:36 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Apr 1 15:15:22 2010 Subject: [Histonet] CAP ANP.22760 Message-ID: <737BD0BF52F0744B96B74B61756AC06441659BF6A8@hestia.ad.pa-ucl.com> Hi Histonetters- "Are new lots of antibody and detection system reagents tested in parallel with old lots? Note: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues." Just wondering how others are handling for the detection kit testing. Thanks Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From marktarango <@t> gmail.com Thu Apr 1 16:12:17 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Apr 1 16:12:21 2010 Subject: [Histonet] Gene Patents Message-ID: Does anyone know if the ruling about BRCA1 and BRCA2 affects MTM labs / Cintec's monopoly on the p16 antibody? Thanks, Mark Tarango From jnocito <@t> satx.rr.com Thu Apr 1 19:42:03 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 1 19:42:15 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: <4bb4d752.23.7e05.848022978@siscom.net> References: <4bb4d752.23.7e05.848022978@siscom.net> Message-ID: <123A95FB8A2B4F0D87D2A8263714B13E@JoePC> I've seen lab get accredited one month, only to have CLIA come through the next month and rip that lab apart. I have been through CAP inspections that consisted of the inspector looking only at slides while he checked off the checklist to inspectors carrying their own thermometers and testing the temp of the paraffin baths on the tissue processors. This is supposed to be a peer review, often it's a witch hunt. I had one CAP team leader tell me that my lab was the last lab he was going to inspect because he dropped out of CAP and went with JCAHO, which was approved by his hospital administrator. Does anyone know why CAP split the "processing" and "grossing parts anyway? I thought it was a stupid idea in the first place. But then again, I've never been known to be politically correct Joe ----- Original Message ----- From: "Andrew Burgeson" To: Sent: Thursday, April 01, 2010 12:26 PM Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com > Sheesh is right, J. > > CAP is all politics as far as I am concerned. It is all > about protecting the careers and paychecks of the general > pathology community. > > I am thouroughly unimpressed with JCAHO, CAP et al. > > If all you need to legally run a laboratory is to be CLIA > inspected, then WHY BOTHER with these subjective entities? > > > The BS I have heard over the last few months concerning MOHS > surgery specimens is one glaring example of the limitations > CAP has in understanding fully certain nuances of the lab > trade. > > Ridiculous. Unless you want the marketing and potential > "perception" that you are better covered from a legal > standpoint, CAP certs are worthless. > > The more I hear about CAP certifications, the more I see it > as a certain community of individuals who are protecting > their perceived "TURF." > > In the end, the pathologists in the group and in the > facility in which you are working have to take > responsibility for these matters. If the docs think a CAP > cert is necessary, then do it and live with it. If not, then > consider yourself lucky to not have to see these people in > your lab. > > I have been through MANY CAP inspections in and out of the > military. For the most part, though, I see people paying > this organization to inspect their lab as the same thing as > "burning a pinch of incense in honor of great Caesar, ruler > of Rome." It will get you some kudos, but tangibly not > change much at all if your pathologists or HR $ hiring hands > want to pocket more $ as a result of hiring "pregnant out of > wedlock 16 year olds" to gross tissue and cut slides. > > Seen it. > > AB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Apr 1 19:46:10 2010 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 1 19:46:19 2010 Subject: [Histonet] Just three months????? In-Reply-To: <72644.18148.qm@web111105.mail.gq1.yahoo.com> References: <72644.18148.qm@web111105.mail.gq1.yahoo.com> Message-ID: I see that now, wasn't the first time I was wrong today, probably wouldn't be the last either ----- Original Message ----- From: "Jeffrey Silverman" To: Sent: Wednesday, March 31, 2010 5:03 PM Subject: [Histonet] Just three months????? CAP is now saying no more gross processing of small things that are entirely submitted- it's all gross examination now whether we mean straining currettings into a cassette or dissecting a complex cancer resection. Dumb as all get out if you ask me. As for the three month training thing, Joe, I'm not so sure about that. They seem to spell out specific amounts of college education required IN ADDITION to training in the laboratory. The requirement first spells out two education choices- 1: an earned associates degree in medical laboratory science OR 2:Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education MUST (caps mine) include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. So in addition to all the college courses, which we all know you need to count and measure six endoscopic biopsies and put them in a cassette, much more training and experience needed than what an unregistered on the job tech needs to orient and embed them properly LOL!!! CLIA then requires that the individual must have (additional) laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. Now there are grandfathering clauses in CLIA which may enable folks (like myself) to continue to gross. I haven't digested that yet, but I'm ready to get that job in Pathmark if necessary. Sheesh, does it ever end? Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From napoli <@t> siscom.net Thu Apr 1 19:58:13 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Apr 1 19:58:21 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Message-ID: <4bb54125.4.41fc.1139194350@siscom.net> It's a turf war....SHARKS AND JETS MAN, SHARKS AND JETS!!! West Side Story played out every day over ego and money. Anyone that tells you they are doing what they do solely for patient care is either deluded or lying. It's not a matter of corrupt...it's a matter of "how corrupt!" Why do plastic surgeons manage melanomas? Why do they like sending specimens to hospital labs? I know... Why are so many gen path labs ignorant of MOHS technique? It's all a glass house. I even live in a glass house. lol From alexandra.meinl <@t> gmail.com Fri Apr 2 04:00:41 2010 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Fri Apr 2 04:00:47 2010 Subject: [Histonet] Assay for cell death needed In-Reply-To: References: Message-ID: Dear all, I want to thank you all for sending suggestions to help us finding an appropriate cell death assay. Your comments were really helpful! Alexandra ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.meinl@trauma.lbg.ac.at From laurie.colbert <@t> huntingtonhospital.com Fri Apr 2 10:19:02 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Apr 2 10:19:07 2010 Subject: [Histonet] Ab Validation Message-ID: <57BE698966D5C54EAE8612E8941D768308453D34@EXCHANGE3.huntingtonhospital.com> When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert From JWeems <@t> sjha.org Fri Apr 2 10:29:43 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Apr 2 10:29:51 2010 Subject: [Histonet] RE: Ab Validation In-Reply-To: <57BE698966D5C54EAE8612E8941D768308453D34@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768308453D34@EXCHANGE3.huntingtonhospital.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015D425240@CHEXCMS10.one.ads.che.org> We save the slide. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, April 02, 2010 11:19 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ab Validation When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mpence <@t> grhs.net Fri Apr 2 11:29:26 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 2 11:29:53 2010 Subject: [Histonet] Ab Validation In-Reply-To: <57BE698966D5C54EAE8612E8941D768308453D34@EXCHANGE3.huntingtonhospital.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DAB@is-e2k3.grhs.net> I just save the paperwork. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, April 02, 2010 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ab Validation When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BoozerKA <@t> ah.org Fri Apr 2 11:41:13 2010 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Fri Apr 2 11:41:43 2010 Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A044@EXCHMBC2.ad.ah.local> Message-ID: <4BB5BBB8.4AA8.00C0.0@ah.org> Just talked to Jim at CAP and he said the term "Processing" still can be the transfer of tissue from one container to another (cassette) to be processed in the tissue processor. If there is any analytical thinking involved (dying margins, measuring...) the process becomes "Grossing" and falls under the high complex rule. Hope that helps. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org >>> 04/01/2010 09:15 >>> I agree Janice. The only thing it looks to me is to now define Grandfathered in. Because it also states "in addition" for that criteria as well. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mahoney,Janice A" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2010 04:10 PM To 'Joe Nocito' , Histonet cc Subject RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines But above that after the education piece it says "in Addition". Jan, Omaha -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, March 31, 2010 4:08 PM To: Mahoney,Janice A; Histonet Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the "processing" and "grossing" pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe ----- Original Message ----- From: "Mahoney,Janice A" To: "Histonet" Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihiggins <@t> gmail.com Fri Apr 2 11:47:06 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Fri Apr 2 11:47:14 2010 Subject: [Histonet] Cleaning VIP Processor containers Message-ID: Hello, Does anyone know a good way to clean the inside of the processor solution station containers (we have a Tissue Tek VIP)? Some of our containers have a film on the sides from years of use. We got what we could reach with a scrub brush, but there are some hard to reach areas. Thanks for your help. Brandi Higgins From flnails <@t> texaschildrens.org Fri Apr 2 11:54:55 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Apr 2 11:55:24 2010 Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines In-Reply-To: <4BB5BBB8.4AA8.00C0.0@ah.org> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A044@EXCHMBC2.ad.ah.local> <4BB5BBB8.4AA8.00C0.0@ah.org> Message-ID: What do you process and don't measure, so everything is grossing. Even our foreskins which are GDO, we take a measurement in our dictation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, April 02, 2010 11:41 AM To: Janice A Mahoney; Kim.Donadio@bhcpns.org; histonet-bounces@lists.utsouthwestern.edu Cc: Histonet; 'Joe Nocito' Subject: RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines Just talked to Jim at CAP and he said the term "Processing" still can be the transfer of tissue from one container to another (cassette) to be processed in the tissue processor. If there is any analytical thinking involved (dying margins, measuring...) the process becomes "Grossing" and falls under the high complex rule. Hope that helps. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org >>> 04/01/2010 09:15 >>> I agree Janice. The only thing it looks to me is to now define Grandfathered in. Because it also states "in addition" for that criteria as well. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mahoney,Janice A" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2010 04:10 PM To 'Joe Nocito' , Histonet cc Subject RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines But above that after the education piece it says "in Addition". Jan, Omaha -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Wednesday, March 31, 2010 4:08 PM To: Mahoney,Janice A; Histonet Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines just had a lively discussion at work. My take is that the only thing CAP changed was that they combined the "processing" and "grossing" pieces together again, which I don't know why they split them in the first place. But you don't have the entire CAP note and many people miss this. The last item states OR three months of documented laboratory training in the high complexity area. Again, my take is that an unregistered histotech can have at least three months of documented training in grossing complex specimens, have the record signed off by the medical director and be ok. How far off am I? Joe ----- Original Message ----- From: "Mahoney,Janice A" To: "Histonet" Sent: Wednesday, March 31, 2010 2:44 PM Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines Is anyone concerned about the new (old) grossing personnel guidelines from CAP. Many labs use people to "process " tissue. No more! ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Jan Mahoney Omaha, NE ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From JMacDonald <@t> mtsac.edu Fri Apr 2 11:55:18 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Apr 2 11:55:25 2010 Subject: [Histonet] Cleaning VIP Processor containers In-Reply-To: Message-ID: dilute acidic acid works wonders on some things. I use vinegar at home for cleaning a multitude of things. Brandi Higgins Sent by: histonet-bounces@lists.utsouthwestern.edu 04/02/2010 09:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Cleaning VIP Processor containers Hello, Does anyone know a good way to clean the inside of the processor solution station containers (we have a Tissue Tek VIP)? Some of our containers have a film on the sides from years of use. We got what we could reach with a scrub brush, but there are some hard to reach areas. Thanks for your help. Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Apr 2 12:06:23 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Apr 2 12:06:30 2010 Subject: [Histonet] Ab Validation In-Reply-To: <57BE698966D5C54EAE8612E8941D768308453D34@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768308453D34@EXCHANGE3.huntingtonhospital.com> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF81D@UWHC-MAIL01.uwhis.hosp.wisc.edu> We save EVERYTHING! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, April 02, 2010 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ab Validation When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Fri Apr 2 12:42:23 2010 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Apr 2 12:42:35 2010 Subject: [Histonet] New CAP question Message-ID: ANP 22760: Are new lots of antibody and detection system reagents tested in parallel with old lots? Note: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues. How is everyone out there handling this? What makes up a panel of control tissues? We are currently doing this by staining a control slide and comparing the results to the previous lot. Looking for guidance. Thank you for your help! Stacy From RCazares <@t> schosp.org Fri Apr 2 13:14:21 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Fri Apr 2 13:14:30 2010 Subject: [SPAM-HC] - [Histonet] New CAP question - Email found in subject In-Reply-To: References: Message-ID: <572D1F45B44B3D4096D554B4CB40639C031FBD5166@EXCHCCRMB.schosp.org> What I have done in my lab, is make up a control block of different tissues and run a vimentin stain with the new detection kit. Then I pull the slide from the previous lot number and compare the two, and document that they are the same. I keep all the validation slides from both detection kits and antibodies in a big binder in plastic slide sheets by antibody name or detection kit (DAB or ALK PHOS)this way the previous lot slide will always be available for comparison. This is the best I could come up with since true parallel testing can not be done on the Ventana Benchmark, the system will not use 2 different dispensers of the same antibody. If anybody has any better suggestions, I 'm sure there are a lot of us that would be interested :) Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Friday, April 02, 2010 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] New CAP question - Email found in subject ANP 22760: Are new lots of antibody and detection system reagents tested in parallel with old lots? Note: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues. How is everyone out there handling this? What makes up a panel of control tissues? We are currently doing this by staining a control slide and comparing the results to the previous lot. Looking for guidance. Thank you for your help! Stacy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From k.fd <@t> live.com Fri Apr 2 13:35:17 2010 From: k.fd <@t> live.com (Karen Doty) Date: Fri Apr 2 13:35:21 2010 Subject: [Histonet] new fluorescent dyes Message-ID: Has anyone tried the Dylight fluorescent dyes? Are they significantly better than the Alexa Fluors? In what ways? I'd really appreciate some opinions before I set up a fluorescent dye project. Thanks very much, Karen Doty University of Illinois Dept. of Veterinary Biosciences _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 From anonwums1 <@t> gmail.com Fri Apr 2 13:40:18 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Apr 2 13:40:22 2010 Subject: [Histonet] new fluorescent dyes In-Reply-To: References: Message-ID: I've tried Dylight 488, Dylight 594, and Dylight 649. They all seem to be pretty bright and don't seem to photobleach much over the course of my experiments. The longest I've looked for signal is about 2 months after the slides were stored in a dark area, and they seem to retain most of their brightness. I've never compared them to the AlexaFluors though, but I'm pretty happy with them. Adam On Fri, Apr 2, 2010 at 1:35 PM, Karen Doty wrote: > > Has anyone tried the Dylight fluorescent dyes? Are they significantly > better than the Alexa Fluors? In what ways? I'd really appreciate some > opinions before I set up a fluorescent dye project. > > > > Thanks very much, > > Karen Doty > > > > University of Illinois > > Dept. of Veterinary Biosciences > > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Charles.Scouten <@t> leica-microsystems.com Fri Apr 2 14:02:08 2010 From: Charles.Scouten <@t> leica-microsystems.com (Charles.Scouten@leica-microsystems.com) Date: Fri Apr 2 14:02:18 2010 Subject: [Histonet] mouse perfusion rate Message-ID: I wonder what pressure you were working at? You can't tell if you control flow rate. It may have been very high. Certainly, it would be possible to put the pressure high enough to produce all the problems you describe, and fluid would come out the nose as a part of that. But how high is that? Is it possible to work at a pressure where fluid comes out the nose, without blowing out the lungs or the liver? I examine only the brain, but a pressure that has fluid coming out the nose and yields excellent brain perfusions is definitely possible (300 mm Hg for about ten seconds or less, then lower pressure fixative flow). And we don't notice anything looking amiss in the rest of the body, which is also perfused. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Thursday, April 01, 2010 8:03 AM To: "Andrea T. Hooper" ; saby_joseph_a@yahoo.com; making@ufl.edu; histonet@lists.utsouthwestern.edu; Charles.Scouten@leica-microsystems.com Subject: RE: [Histonet] mouse perfusion rate Someone I believe on Histonet a couple years ago told me the same thing. The "perfusion circuit" is compromised if you have fluid coming out the nose and internal organs swelling up... Merced --On Wednesday, March 31, 2010 4:14 PM -0700 "Andrea T. Hooper" < andreahooper@rocketmail.com> wrote: > > Very interesting! Coming out the nose is definitely bad for any work I > have done in the past - lungs get blown out, liver doesn't perfuse well > and bone marrow looks horrific. However, if you are working with PFA and > doing a post-fix anyway, you will probably be fine. If you are using GA > and counting on the perfusion to ensure excellent fixation for things > such as lacZ staining (b/c post-fix in GA never works well for bone or > deep into tissues) then blowing it out the nose is bad. Very bad. > > Andrea > > > --- On Mon, 3/29/10, Charles.Scouten@leica-microsystems.com > < Charles.Scouten@leica-microsystems.com> wrote: > > > From: Charles.Scouten@leica-microsystems.com > < Charles.Scouten@leica-microsystems.com> > Subject: RE: [Histonet] mouse perfusion rate > To: leiker@buffalo.edu, saby_joseph_a@yahoo.com, making@ufl.edu, > histonet@lists.utsouthwestern.edu > Date: Monday, March 29, 2010, 6:26 PM > > > I have perfused mice and rats at 300 mm Hg, about double physiological > level, don't know what that made the flow rate. All mammals have the > same blood pressure (within tolerances), so it is easier to select a > suitable pressure to use than a flow rate, which varies dramatically. > > > > I look at brain, never pay any attention to the gut. Clear fluid comes > out the nose, that is a good sign. There are pressure release valves > across the cribiform plate to release CSF if there is too much. I am > flooding the system, fluid coming out the nose means the extracellular > fluid and CSF is being replaced as well as vascular blood. Good. > > > > The tissue is quality is excellent, free of red blood cells, can be > unshrunk depending on the tonicity (should be sub isotonic) of the > fixative fluid. Have looked at Nissl and EM material, no evidence of > damage to the tissue. > > > > If gut is extended, might have something to do with the large intestines > job of removing fluid from feces, and flooding the system swells the > tissue. But does it matter? Do you use that tissue? What is the > tissue quality if you use it after physiological pressure perfusion. > > > > Cordially, > > Charles W. Scouten, Ph.D > > Product Manager, MNL > > Biosystems Division > > > > Leica Biosystems Richmond, Inc. > 5205 Route 12 > P.O. Box 528 > Richmond, IL 60071 > United States of America > > Telephone 630 964 0501 > > facsimile +1 630 964 0576 > > www.MyNeuroLab.com < http://www.myneurolab.com/> > > www.leica-microsystems.com < http://www.leica-microsystems.com/> > > > > IMPORTANT - This email and any attachments may be confidential. Any > retransmissions, dissemination or other use of > > these materials by persons or entities other than the intended recipient > is prohibited. If received in error, please contact > > us and delete all copies. Before opening or using attachments, check > them for viruses and defects. Our liability is limited > > to resupplying any affected attachments. [Any representations or > opinions expressed in this email are those of the > > individual sender]. > > > > > > From: Merced M Leiker < leiker@buffalo.edu> [mailto:Merced M Leiker > < leiker@buffalo.edu>] > Sent: Monday, March 29, 2010 9:05 AM > To: Joseph Saby < saby_joseph_a@yahoo.com>; > Charles.Scouten@leica-microsystems.com; making@ufl.edu; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] mouse perfusion rate > > > > Hi Joe, > > Thanks for that notice about flow rates. But I think for the mouse you > meant 1-3mls/min (not per 10min?)... > > Regards, > Merced > > --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby > < saby_joseph_a@yahoo.com> wrote: > >> >> >> All- >> >> From previous work with rat perfusions, the flow rate was about 10 >> ml/minute. If I had to guess, the equivalent flow rate for a mouse > would >> be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will >> definitely cause blowout artefacts. >> >> Joe Saby, BA HT >> >> >> >> >> __________________________________________________ >> From: Merced M Leiker < leiker@buffalo.edu> >> To: Charles.Scouten@leica-microsystems.com; making@ufl.edu; >> histonet@lists.utsouthwestern.edu >> Sent: Fri, March 19, 2010 9:21:38 AM >> Subject: RE: [Histonet] mouse perfusion rate >> >> The vasculature will leak too much and the mouse will get bloated - >> you'll >> see it first in either the intestines blowing up like a balloon or > fluid >> coming out of the nose. Just not the same as the heart pumping when > the >> mouse is alive with intact physiology and normal functioning. Don't > know >> exactly why, but that's what happens when you go too fast. Perhaps the >> vasculature has lost its control to compensate for the pressure? I'm > not >> a >> physiologist so I'm not sure why...maybe someone on the Histonet can >> answer >> that? >> >> Regards, >> Merced >> >> --On Thursday, March 18, 2010 5:49 PM -0500 >> Charles.Scouten@leica-microsystems.com wrote: >> >>> >>> >>> Why not? What happens? One would think the mammalian cardiovascular >>> system could withstand physiological pressures and flow rates, at > least >>> for one lifetime? >>> >>> >>> >>> >>> Cordially, >>> >>> Charles W. Scouten, Ph.D >>> >>> Product Manager, MNL >>> >>> Biosystems Division >>> >>> >>> >>> Leica Biosystems Richmond, Inc. >>> 5205 Route 12 >>> P.O. Box 528 >>> Richmond, IL 60071 >>> United States of America >>> >>> Telephone 630 964 0501 >>> >>> facsimile +1 630 964 0576 >>> >>> www.MyNeuroLab.com >>> >>> www.leica-microsystems.com >>> >>> >>> >>> IMPORTANT - This email and any attachments may be confidential. Any >>> retransmissions, dissemination or other use of >>> >>> these materials by persons or entities other than the intended > recipient >>> is prohibited. If received in error, please contact >>> >>> us and delete all copies. Before opening or using attachments, check > them >>> for viruses and defects. Our liability is limited >>> >>> to resupplying any affected attachments. [Any representations or > opinions >>> expressed in this email are those of the >>> >>> individual sender]. >>> >>> >>> >>> >>> >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Merced M >>> Leiker < leiker@buffalo.edu> >>> Sent: Thursday, March 18, 2010 12:38 PM >>> To: MKing < making@ufl.edu>; histonet@lists.utsouthwestern.edu >>> Subject: Re: [Histonet] mouse perfusion rate >>> >>> >>> >>> That may be mouse cardiac output, but I can assure you, from > experience, >>> you do not want to perfuse at 17ml/min. >>> >>> Regards, >>> Merced >>> >>> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making@ufl.edu> >>> wrote: >>> >>>> Li, >>>> >>>> Mouse cardiac output seems to be about 17 ml/min (e.g. >>>> www.transonic.com/mice1.shtml), you probably want to try for that to >>>> keep pressures close to physiological. >>>> A syringe pump is pretty inexpensive and probably all you need. >>>> >>>> Mike >>>> >>>> ----- Original Message ----- >>>> From: Li Zhang < dancingwing@yahoo.com> >>>> Date: Wednesday, March 17, 2010 14:59 >>>> Subject: [Histonet] question about mouse perfusion >>>> To: histonet@lists.utsouthwestern.edu >>>> >>>> > > My question is: can anyone give me a rough idea of how fast I >>>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3 >>>> > > min, and I suspect that it's too fast because I do observe >>>> > > tissue swelling sometimes. >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> Merced M Leiker >>> Research Technician III >>> Cardiovascular Medicine >>> 348 Biomedical Research Building >>> State University of New York at Buffalo >>> 3435 Main St, Buffalo, NY 14214 USA >>> leiker@buffalo.edu >>> 716-829-6118 (Ph) >>> 716-829-2665 (Fx) >>> >>> No trees were harmed in the sending of this email. >>> However, many electrons were severely inconvenienced. >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > ______________________________________________________________________ >>> This email has been scanned by the MessageLabs Email Security System. >>> For more information please visit http://www.messagelabs.com/email >>> > ______________________________________________________________________ >> >> >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From september.amspacher <@t> bassett.org Fri Apr 2 14:24:08 2010 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Fri Apr 2 14:24:14 2010 Subject: [Histonet] Embedding Beads Message-ID: <71A87F8FEFFB024DB88B064543C7F24B64A34D0B@ex2.bassett.org> Hello out there in Histoland- I am looking for embedding beads, if you happen to know a place to buys them please let me know. Thanks. TGIF September Amspacher HT(ASCP) Technical Specialist - Histology Department Laboratory Chemical Hygiene Officer Bassett Medical Center Cooperstown, New York From LINDA.MARGRAF <@t> childrens.com Fri Apr 2 14:59:40 2010 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri Apr 2 14:59:47 2010 Subject: [Histonet] job posting Message-ID: <4BB6065C.F783.00DA.0@childrens.com> Here's a message I was asked to post to the list.......(please respond to Dr Mackinnon, not me. thanks) A position for a histotechnologist in a combined clinical molecular diagnostics/ translational research lab at the Medical College of Wisconsin in Milwaukee is available for July 1, 2010. The main responsibilities will be to support all histology and IHC for research projects within the department of pathology. This includes tissue microarrays (TMA) construction, establishing IHC protocols, and preparation of written procedure manuals with routine updates. The lab will also perform molecular-based assays on tissue samples, FISH, and CISH. To learn more about this position, please send your CV to me. Craig Mackinnon, MD, PhD a.craig.mackinnon@gmail.com Please consider the environment before printing this e-mail

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From JHowery2 <@t> yrmc.org Fri Apr 2 12:13:24 2010 From: JHowery2 <@t> yrmc.org (Howery, Jeffrey) Date: Fri Apr 2 15:02:01 2010 Subject: [Histonet] RCC Message-ID: What are people using for a Renal cell Carcinoma. ( this is a test ) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From marktarango <@t> gmail.com Fri Apr 2 15:11:03 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 2 15:11:12 2010 Subject: [Histonet] RCC In-Reply-To: References: Message-ID: If you mean what control do we use for our anti-RCC stain, we use a piece of normal kidney and clear cell renal cell carcinoma. If you were asking about where we purchase the antibody, we get it from Cell Marque. If you wondering about a protocol we use the Ventana Benchmark. Protease 1 for 8 minutes then 8 minutes with anti-RCC, counterstain and bluing. Mark Tarango On Fri, Apr 2, 2010 at 10:13 AM, Howery, Jeffrey wrote: > What are people using for a Renal cell Carcinoma. ( this is a test ) > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From napoli <@t> siscom.net Fri Apr 2 15:19:59 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Fri Apr 2 15:20:03 2010 Subject: [Histonet] 4BB5BBB8.4AA8.00C0.0@ah.org> Message-ID: <4bb6516f.1ff.66a4.1718151261@siscom.net> Kathy, What you mentioned to me is completely inconsistent with what I learned when I was in training. ANYONE touching the tissue, especially taking it out of a specimen container and transferring it to a processing cassette, is by definition "GROSSING!" Gross description (dimensions, color, consistency, friability, etc etc etc) are all included. Every specimen should at least be getting a gross description, even if it isn't processed!!! (Example...foreign body, like a rock or a BB or a stinger from an arthropod, or any foreign object) I seriously question the validity of the quoted CAP standard by this person. Who out there manipulates tissue and doesnt have to describe it? Once again, this I would call a "glaring" example of the nebulous nature of CAP standards sometimes and the arbitrary interpretations that occur within the organization (and ones like it, depending upon the individual inspector or CAP staffer you talk to. YOU SEE....that is what is really the FACT in all of this discussion. It's all subjective....just like legal interpretations. So why does CAP get to be in the bully-pulpit, pedantically "pontificating" to the pathology community as to "HOW ITS "SUPPOSED" to be done?" Regards, AB From JHowery2 <@t> yrmc.org Fri Apr 2 15:57:26 2010 From: JHowery2 <@t> yrmc.org (Howery, Jeffrey) Date: Fri Apr 2 15:57:30 2010 Subject: [Histonet] CD5 Message-ID: Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From pathmaster <@t> yahoo.com Fri Apr 2 16:34:41 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Fri Apr 2 16:34:44 2010 Subject: [Histonet] Fw: Validation- Keeping track of everything Message-ID: <281878.18479.qm@web111109.mail.gq1.yahoo.com> --- On Fri, 4/2/10, Jeffrey Silverman wrote: From: Jeffrey Silverman Subject: Validation- Keeping track of everything To: laurie.colbert@huntingtonhospital.com Date: Friday, April 2, 2010, 5:34 PM Better stick em in a box labelled with stain date and lot number being validated. Someone's going to want to see them. Here in New York, they are going nuts with new regs. We now have to assign lot numbers to control blocks after we validate them. It doesn't matter that we stick the control on each and every slide we stain and one can plainly determine if the control is adequate or not. If it's not, it has no business being part of the patient record. Now we have to give it a number and track it. Dumb ass stuff if you ask me. In the old days, we'd cut a control slide and if it worked it worked you kept using it and you used it up. If it didn't work, the pathologist would have rejected it and ordered a repeat with a good control. The proof was on each and every slide that it worked. Antibodies and detection chemistries are perishable and can be capricious so validation and inventory tracking are worthwhile IMHO. But fer goodness sake, now we must record the lot numbers of every batch of xylene, alcohol, eosin, etc and track service dates etc. When was the last time anyone had problems with bulk xylene, alcohol, etc. We already do a meticulous daily QC check on the slides, if there's a problem, one can go and check the lot number in use currently, record it and take appropriate action. But, noooooooo, the bureaucrats need more to do, so now we must record the lot number of everything that comes in and track the dates that we use it. And they wonder why healthcare is a financial leech bleeding the nation and it's people. I reiterate, sheesh. Happy Springtime? everyone. Enjoy the holiday.? Jeff Silverman From pathmaster <@t> yahoo.com Fri Apr 2 16:41:09 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Fri Apr 2 16:41:15 2010 Subject: [Histonet] (no subject) Message-ID: <864789.24389.qm@web111108.mail.gq1.yahoo.com> Bleach works great for the fixative and early alcohol series containers, , dilute 50/50 with water and swish it around, let it sit a while. If it's yellow fatty slime in the later absolute alcohol baths,? you must rinse the containers with 50-100 ml of absolute and repeat until a bit of the swill mixed with water doesn't turn white. Oh the fun days of scut work. Jeff From pathmaster <@t> yahoo.com Fri Apr 2 16:51:02 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Fri Apr 2 16:51:05 2010 Subject: [Histonet] Good news about grossing Message-ID: <682261.48137.qm@web111106.mail.gq1.yahoo.com> Or is it????? We measure everything. I think the benchmark should be, if you submit it intact and in its entirety, with no cutting or dissection and no judgement about which or how much to submit,? then counting, measuring, weighing, and cassetting biospies or straining or scraping curettings together into a screen counts as processing and requires only basic training and knowlege.? But I have the feeling that as long as the pathologist delineates what may be done by whom with what degree of supervision,? and regularly evaluates the work of these individuals, that everything will be OK on any inspection. Just be prepared to prove it. >>>>>>>Just talked to Jim at CAP and he said the term "Processing" still can be the transfer of tissue from one container to another (cassette) to be processed in the tissue processor.? If there is any analytical thinking involved (dying margins, measuring...) the process becomes "Grossing" and falls under the high complex rule. Hope that helps. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR? 97216 boozerka@ah.org From disbrc <@t> shands.ufl.edu Fri Apr 2 18:52:18 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Fri Apr 2 18:52:23 2010 Subject: [Histonet] antibody validation Message-ID: <4BB64AF1.72AC.0059.1@shands.ufl.edu> HI Everyone and Happy Easter! Just wondering how you validate your antibodies? I've read that some antibodies use negative controls with the genes or proteins removed and that the positive controls have the protein added. Is that the procedure for research or manufacturing situations? Thanks, Carrie From tahseen <@t> brain.net.pk Fri Apr 2 23:07:38 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Fri Apr 2 23:08:46 2010 Subject: [Histonet] Removal of carbon pigment Message-ID: <31959.202.125.145.178.1270267658.squirrel@brain.net.pk> Hellow all. We have received CT guided FANA smears from lung .The siles are full of carbon particles that mask the under histiocytes and granulomata.W require the removal of carbor particles.Kindly help out to remove them. Muhammad Tahseen Supervisor Histology Javed uz zaman Supervisor Cytology SKMT PAKISTAN LAHORE From wdesalvo.cac <@t> hotmail.com Sat Apr 3 03:17:29 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Sat Apr 3 03:17:34 2010 Subject: [Histonet] Embedding Beads In-Reply-To: <71A87F8FEFFB024DB88B064543C7F24B64A34D0B@ex2.bassett.org> References: <71A87F8FEFFB024DB88B064543C7F24B64A34D0B@ex2.bassett.org> Message-ID: I suggest trying a less expensive and easy to use identifier, "party confetti". You can purchase on the internet and buy individual packages in specific shapes and colors. We have over 50 individuals w/ an assined shape/color and it works great. www.shindigz.com www.chicoparty.com www.confetti.com William DeSalvo, B.S., HTL(ASCP) > From: september.amspacher@bassett.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 2 Apr 2010 15:24:08 -0400 > Subject: [Histonet] Embedding Beads > > Hello out there in Histoland- I am looking for embedding beads, if you happen to know a place to buys them please let me know. Thanks. TGIF > September Amspacher HT(ASCP) > Technical Specialist - Histology Department > Laboratory Chemical Hygiene Officer > Bassett Medical Center > Cooperstown, New York > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 From settembr <@t> umdnj.edu Mon Apr 5 05:30:51 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Apr 5 05:31:09 2010 Subject: [Histonet] Ab Validation Message-ID: We have started saving these since the "NEW" question came up in June of 2009. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Laurie Colbert 04/02/10 11:19 AM >>> When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From matt <@t> techoneweb.com Mon Apr 5 07:37:56 2010 From: matt <@t> techoneweb.com (Matthew Mincer) Date: Mon Apr 5 07:35:45 2010 Subject: [Histonet] Histobath Message-ID: <4BB9D9A4.6020203@techoneweb.com> Does anyone know the "normal" operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 From k84as <@t> yahoo.com Mon Apr 5 07:46:25 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Mon Apr 5 07:46:28 2010 Subject: [Histonet] Happy Easter! Message-ID: <794795.2296.qm@web112616.mail.gq1.yahoo.com> Dear histonetters I wish to you, Happy Holidays with a lot of peace and health! dr.Mohamed Abd el razik Histology Dep. Faculty of Vet. Med. Cairo University- Egypt From malbenatti <@t> googlemail.com Mon Apr 5 07:47:08 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Mon Apr 5 07:47:15 2010 Subject: [Histonet] Histobath In-Reply-To: <4BB9D9A4.6020203@techoneweb.com> References: <4BB9D9A4.6020203@techoneweb.com> Message-ID: Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 benatm@gosh.nhs.uk On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer wrote: > Does anyone know the "normal" operating temperature of the Thermo > Histobath? A customer has asked me to repair theirs and I want to make sure > I get the temp correct. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Service > 159 N Marion Street > PMB163 > Oak Park, IL 60301 > office (708) 383-6040 X 10 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From matt <@t> techoneweb.com Mon Apr 5 07:56:19 2010 From: matt <@t> techoneweb.com (Matthew Mincer) Date: Mon Apr 5 07:54:03 2010 Subject: [Histonet] Histobath part 2 Message-ID: <4BB9DDF3.3080903@techoneweb.com> I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 From lpaveli1 <@t> hurleymc.com Mon Apr 5 08:03:04 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Apr 5 08:03:18 2010 Subject: [Histonet] Histobath part 2 In-Reply-To: <4BB9DDF3.3080903@techoneweb.com> References: <4BB9DDF3.3080903@techoneweb.com> Message-ID: <4BB9A748.59CD.00EE.0@hurleymc.com> Matt, I have a copy of the instructional/operational manual from my histobath that we purchased in 1994 from Shandon-Lipshaw. The specifications for its lowest temperature is -60C. The liquid that is used is 2-methylbutane (isopentane). I can fax you a copy if it would help. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> Matthew Mincer 4/5/2010 8:56 AM >>> I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From malbenatti <@t> googlemail.com Mon Apr 5 07:59:06 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Mon Apr 5 08:05:23 2010 Subject: [Histonet] Histobath In-Reply-To: <4BB9DCDE.1060005@techoneweb.com> References: <4BB9D9A4.6020203@techoneweb.com> <4BB9DCDE.1060005@techoneweb.com> Message-ID: Sorry for that Matt, In this case the internal temperature of the cryostat chamber should be set at -20 to -22 degree Celsius, not sure what would the actual temperature of the histobath itself but our thermo scientific cryostat has the following settings: - the cryobar is set at -18 Degree Celsius, - the Cryo chamber at - 22 degree Celsius. Hope this help Malika Benatti Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 benatm@gosh.nhs.uk - Show quoted text - On Mon, Apr 5, 2010 at 1:51 PM, Matthew Mincer wrote: > Thanks, but the Histobath I was talking about is a freezing device for > cryostats. > > Matt > > Malika Benatti wrote: > >> Hi Matt, >> >> Depending on the wax melting point which should be around 57 degree >> Celsius, your water bath should be set at 50 degree Celsius. >> >> Hope this help. >> >> Malika Benatti BSc MIBMS >> Specialist Biomedical Scientist >> Great Ormond Street Children Hospital >> London, WC1N 3JH >> UK >> >> Tel: (+44) 0207 4059200 Ext 5475 >> benatm@gosh.nhs.uk >> >> >> >> >> >> On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer > matt@techoneweb.com>> wrote: >> >> Does anyone know the "normal" operating temperature of the Thermo >> Histobath? A customer has asked me to repair theirs and I want to >> make sure I get the temp correct. >> >> Thanks >> Matt >> >> -- Matthew Mincer >> Tech One Biomedical Service >> 159 N Marion Street >> PMB163 >> Oak Park, IL 60301 >> office (708) 383-6040 X 10 >> cell (708) 822-3738 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> -- >> " Smile .... it confuses people " >> > > > -- > Matthew Mincer > Tech One Biomedical Service > 159 N Marion Street > PMB163 > Oak Park, IL 60301 > office (708) 383-6040 X 10 > cell (708) 822-3738 > > -- " Smile .... it confuses people " From Janice.Mahoney <@t> alegent.org Mon Apr 5 08:08:17 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Apr 5 08:08:43 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: <123A95FB8A2B4F0D87D2A8263714B13E@JoePC> References: <4bb4d752.23.7e05.848022978@siscom.net> <123A95FB8A2B4F0D87D2A8263714B13E@JoePC> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A050@EXCHMBC2.ad.ah.local> Talk about politically incorrect. "Pregnant, out of wedlock 16 year olds?" Are you kidding me? Seriously AB? Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 01, 2010 7:42 PM To: Andrew Burgeson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com I've seen lab get accredited one month, only to have CLIA come through the next month and rip that lab apart. I have been through CAP inspections that consisted of the inspector looking only at slides while he checked off the checklist to inspectors carrying their own thermometers and testing the temp of the paraffin baths on the tissue processors. This is supposed to be a peer review, often it's a witch hunt. I had one CAP team leader tell me that my lab was the last lab he was going to inspect because he dropped out of CAP and went with JCAHO, which was approved by his hospital administrator. Does anyone know why CAP split the "processing" and "grossing parts anyway? I thought it was a stupid idea in the first place. But then again, I've never been known to be politically correct Joe ----- Original Message ----- From: "Andrew Burgeson" To: Sent: Thursday, April 01, 2010 12:26 PM Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com > Sheesh is right, J. > > CAP is all politics as far as I am concerned. It is all > about protecting the careers and paychecks of the general > pathology community. > > I am thouroughly unimpressed with JCAHO, CAP et al. > > If all you need to legally run a laboratory is to be CLIA > inspected, then WHY BOTHER with these subjective entities? > > > The BS I have heard over the last few months concerning MOHS > surgery specimens is one glaring example of the limitations > CAP has in understanding fully certain nuances of the lab > trade. > > Ridiculous. Unless you want the marketing and potential > "perception" that you are better covered from a legal > standpoint, CAP certs are worthless. > > The more I hear about CAP certifications, the more I see it > as a certain community of individuals who are protecting > their perceived "TURF." > > In the end, the pathologists in the group and in the > facility in which you are working have to take > responsibility for these matters. If the docs think a CAP > cert is necessary, then do it and live with it. If not, then > consider yourself lucky to not have to see these people in > your lab. > > I have been through MANY CAP inspections in and out of the > military. For the most part, though, I see people paying > this organization to inspect their lab as the same thing as > "burning a pinch of incense in honor of great Caesar, ruler > of Rome." It will get you some kudos, but tangibly not > change much at all if your pathologists or HR $ hiring hands > want to pocket more $ as a result of hiring "pregnant out of > wedlock 16 year olds" to gross tissue and cut slides. > > Seen it. > > AB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. 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From b-frederick <@t> northwestern.edu Mon Apr 5 09:00:58 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 5 09:01:09 2010 Subject: [Histonet] RCC In-Reply-To: Message-ID: <6D9B42048B2E43C4AEEC2B630377C443@lurie.northwestern.edu> RCC from Novacastra (Leica) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey Sent: Friday, April 02, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC What are people using for a Renal cell Carcinoma. ( this is a test ) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Apr 5 09:06:17 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Apr 5 09:07:23 2010 Subject: [Histonet] RE: CD5 In-Reply-To: References: Message-ID: High pH Retrieval for 20 minutes in the PT module 20 minute incubation of CD5 15 minute mouse linker 20 Minute incubation Flex HRP 10 minute DAB 5 minute Hematoxylin Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cparsons <@t> vt.edu Mon Apr 5 09:26:08 2010 From: cparsons <@t> vt.edu (Parsons, Catherine) Date: Mon Apr 5 09:25:43 2010 Subject: [Histonet] Choosing Carousel Style Tissue Processors Message-ID: I am in the process of selecting a new tissue processor for my lab, and have narrowed the search to two models. Both are carousel style tissue processors. We are a small, low volume research lab, so the carousel style fits well with our processing and space needs. I would appreciate any information/opinions that anyone has about these two models and manufacturers. Major lab equipment purchases seem to be on a 20 year cycle here, so I need to choose carefully and well! My choices are the Leica TP 1020 and the Thermo Scientific Microm STP 120 Spin tissue processor. Has anyone found the spin function on the Microm unit to be useful, or just another part to break down and need replacement? Thanks in advance for any information that you can offer. Cathy Parsons cparsons@vt.edu From GDawson <@t> dynacaremilwaukee.com Mon Apr 5 09:55:30 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Apr 5 09:55:35 2010 Subject: [Histonet] RE: CD5 In-Reply-To: Message-ID: Jeff, Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue# RM-9119-S. Concentration of 1:75 diluted w/Dako diluent. Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down. 35 minute primary antibody application. 30 minute Envision+ polyclonal (DAKO) secondary antibody detection. 7 minute DAB+ (DAKO). Counter-stain as you like. Results on this antibody are excellent. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From napoli <@t> siscom.net Mon Apr 5 12:03:18 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Mon Apr 5 12:03:26 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Message-ID: <4bba17d6.38a.2b93.692087890@siscom.net> The point is not about gender, as I stated before... It's about a person's health risks and lack of training overlooked for the sake of labor. TYVM From marktarango <@t> gmail.com Mon Apr 5 12:18:46 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Apr 5 12:18:52 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: <4bba17d6.38a.2b93.692087890@siscom.net> References: <4bba17d6.38a.2b93.692087890@siscom.net> Message-ID: When I was 16 years-old I was grossing in the lab. We had very busy pathologists (busy reading slides) who thought it was okay to train their courier (me at the time) to gross. I had already been there handing them bottles and closing cassette lids for several months. When we had our first CLIA inspection, they had me intial and sign paperwork saying that I had grossed so many cases of various specimen types under patholgist supervision and had been grossing so long. The problem was that I was only a high school graduate at the time. They then changed direction and told the inspectors that the pathologists did all the grossing. Just brings back memories. Thought I'd share. Mark Tarango On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson wrote: > The point is not about gender, as I stated before... > > It's about a person's health risks and lack of training > overlooked for the sake of labor. TYVM > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Mon Apr 5 12:23:13 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Apr 5 12:23:16 2010 Subject: [Histonet] Re: Histobath Message-ID: I doubt you'll be successful in repairing a Histobath, particularly since the device is no longer in manufacture. There are some alternative instruments - see the Histonet archives for more information them and about liquid refrigerants used in them. 2-methylbutane (isopentane) and acetone are serious fire hazard, even at very low temperatures. Asi I have before, I suggest looking into 3M Novec Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 It melts and freezes at -135 C, so that it would remain liquid in the Histobath. (It would freeze solid in liquid nitrogen, however.) It boils at 60 C., and is listed as non-flammable. It cost around $230 a gallon a few years ago. Bob Richmond Samurai Pathologist Knoxville TN From sbreeden <@t> nmda.nmsu.edu Mon Apr 5 12:33:50 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Apr 5 12:33:55 2010 Subject: [Histonet] Histology Stories Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46FE8@nmdamailsvr.nmda.ad.nmsu.edu> To all of you who sent me your "how did I get started in histology" stories, I have not forgotten you! I am making all your contributions into one document and although I have about a dozen left to transcribe, if you will contact me DIRECTLY at nmhisto@comcast.net, I will send you the entire document when I finish it. Taken as a whole, it's pretty amazing/interesting/involved/riveting! Please do NOT contact me via Histonet - use my home email as above. My plan is to submit the stories to NSH to use as a tool for recruiting (it seems that most of us got our start through karma!). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From malbenatti <@t> googlemail.com Mon Apr 5 13:03:39 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Mon Apr 5 13:03:46 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: References: <4bba17d6.38a.2b93.692087890@siscom.net> Message-ID: I am very confuse reading every email reply to this tread also I would be really grateful if someone could enlighten with regard to what is the comment practice in the US. Having been trained as a histotechnologist although we are call Specialist Biomedical Scientist in the UK, we cannot practice unless we are fully registered with the Health Professional Council HPC, which I believe has the same role as the ASCP. Every 2 years we may be audited a demonstrate that we fully comply with HPC regulation and CPD or lose or registration. All laboratories are accredited by the Clinical Pathology Accreditation CPA under the international organization for standardization legislation (ISO 15189). Laboratory accreditation happen every 2 years cycle for which the laboratory has to comply with a set of standard. During inspection accessor review everything with a fine tooth comb, and score you some of the issues may just be minors but they will always get you with a critical issue, which you will have a set amount of time to correct, they will then return and verify that all non compliance and critical issues have been address before giving you CPA accreditation status. Having a 16 years old out of school with little experience in histology and no formal training grossing specimen is never heard off, only Register Biomedical Scientist are allowed to do small biopsies, Advance Practitioner, Trainee Pathologist, will be involved in the grossing of lager specimens, and tumour specimens. On Mon, Apr 5, 2010 at 6:18 PM, Mark Tarango wrote: > When I was 16 years-old I was grossing in the lab. We had > very busy pathologists (busy reading slides) who thought it was okay to > train their courier (me at the time) to gross. I had already been there > handing them bottles and closing cassette lids for several months. > > When we had our first CLIA inspection, they had me intial and sign > paperwork > saying that I had grossed so many cases of various specimen types under > patholgist supervision and had been grossing so long. The problem was that > I was only a high school graduate at the time. They then changed direction > and told the inspectors that the pathologists did all the grossing. > > Just brings back memories. Thought I'd share. > > Mark Tarango > On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson > wrote: > > > The point is not about gender, as I stated before... > > > > It's about a person's health risks and lack of training > > overlooked for the sake of labor. TYVM > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From Erik.Dokken <@t> onassignment.com Mon Apr 5 13:14:15 2010 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Mon Apr 5 13:18:09 2010 Subject: [Histonet] Histotech Position in Bay Area is Still Open. In-Reply-To: <201004051704.o35H1HkY023182@smtp11.onasgn.net> Message-ID: On Assignment Healthcare is currently looking for a Histo Tech for a contract assignment here in the Bay Area. This assignment would start ASAP and will be for an indefinite period of time as it is to covering for a leave of absence. Hours are: Monday through Friday, 5:30am to 2:00pm. For additional information please contact me via email. Erik Dokken Market Leader - Northwest Region www.oahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, April 05, 2010 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 77, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Ab Validation (Dana Settembre) 2. Histobath (Matthew Mincer) 3. Happy Easter! (mohamed abd el razik) 4. Re: Histobath (Malika Benatti) 5. Histobath part 2 (Matthew Mincer) 6. Re: Histobath part 2 (Lynette Pavelich) 7. Re: Histobath (Malika Benatti) 8. RE: 72644.18148.qm@web111105.mail.gq1.yahoo.com (Mahoney,Janice A) 9. RE: RCC (Bernice Frederick) 10. RE: CD5 (McMahon, Loralee A) 11. Choosing Carousel Style Tissue Processors (Parsons, Catherine) 12. RE: CD5 (Dawson, Glen) ---------------------------------------------------------------------- Message: 1 Date: Mon, 05 Apr 2010 06:30:51 -0400 From: "Dana Settembre" Subject: Re: [Histonet] Ab Validation To: "Laurie Colbert" , Message-ID: Content-Type: text/plain; charset=US-ASCII We have started saving these since the "NEW" question came up in June of 2009. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Laurie Colbert 04/02/10 11:19 AM >>> When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 05 Apr 2010 07:37:56 -0500 From: Matthew Mincer Subject: [Histonet] Histobath To: histonet@lists.utsouthwestern.edu Message-ID: <4BB9D9A4.6020203@techoneweb.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone know the "normal" operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 ------------------------------ Message: 3 Date: Mon, 5 Apr 2010 05:46:25 -0700 (PDT) From: mohamed abd el razik Subject: [Histonet] Happy Easter! To: Histonet@lists.utsouthwestern.edu Message-ID: <794795.2296.qm@web112616.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear histonetters I wish to you, Happy Holidays with a lot of peace and health! dr.Mohamed Abd el razik Histology Dep. Faculty of Vet. Med. Cairo University- Egypt ------------------------------ Message: 4 Date: Mon, 5 Apr 2010 13:47:08 +0100 From: Malika Benatti Subject: Re: [Histonet] Histobath To: Matthew Mincer Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 benatm@gosh.nhs.uk On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer wrote: > Does anyone know the "normal" operating temperature of the Thermo > Histobath? A customer has asked me to repair theirs and I want to make sure > I get the temp correct. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Service > 159 N Marion Street > PMB163 > Oak Park, IL 60301 > office (708) 383-6040 X 10 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " ------------------------------ Message: 5 Date: Mon, 05 Apr 2010 07:56:19 -0500 From: Matthew Mincer Subject: [Histonet] Histobath part 2 To: histonet@lists.utsouthwestern.edu Message-ID: <4BB9DDF3.3080903@techoneweb.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 ------------------------------ Message: 6 Date: Mon, 05 Apr 2010 09:03:04 -0400 From: "Lynette Pavelich" Subject: Re: [Histonet] Histobath part 2 To: , "Matthew Mincer" Message-ID: <4BB9A748.59CD.00EE.0@hurleymc.com> Content-Type: text/plain; charset=US-ASCII Matt, I have a copy of the instructional/operational manual from my histobath that we purchased in 1994 from Shandon-Lipshaw. The specifications for its lowest temperature is -60C. The liquid that is used is 2-methylbutane (isopentane). I can fax you a copy if it would help. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> Matthew Mincer 4/5/2010 8:56 AM >>> I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 5 Apr 2010 13:59:06 +0100 From: Malika Benatti Subject: Re: [Histonet] Histobath To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Sorry for that Matt, In this case the internal temperature of the cryostat chamber should be set at -20 to -22 degree Celsius, not sure what would the actual temperature of the histobath itself but our thermo scientific cryostat has the following settings: - the cryobar is set at -18 Degree Celsius, - the Cryo chamber at - 22 degree Celsius. Hope this help Malika Benatti Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 benatm@gosh.nhs.uk - Show quoted text - On Mon, Apr 5, 2010 at 1:51 PM, Matthew Mincer wrote: > Thanks, but the Histobath I was talking about is a freezing device for > cryostats. > > Matt > > Malika Benatti wrote: > >> Hi Matt, >> >> Depending on the wax melting point which should be around 57 degree >> Celsius, your water bath should be set at 50 degree Celsius. >> >> Hope this help. >> >> Malika Benatti BSc MIBMS >> Specialist Biomedical Scientist >> Great Ormond Street Children Hospital >> London, WC1N 3JH >> UK >> >> Tel: (+44) 0207 4059200 Ext 5475 >> benatm@gosh.nhs.uk >> >> >> >> >> >> On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer > matt@techoneweb.com>> wrote: >> >> Does anyone know the "normal" operating temperature of the Thermo >> Histobath? A customer has asked me to repair theirs and I want to >> make sure I get the temp correct. >> >> Thanks >> Matt >> >> -- Matthew Mincer >> Tech One Biomedical Service >> 159 N Marion Street >> PMB163 >> Oak Park, IL 60301 >> office (708) 383-6040 X 10 >> cell (708) 822-3738 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> -- >> " Smile .... it confuses people " >> > > > -- > Matthew Mincer > Tech One Biomedical Service > 159 N Marion Street > PMB163 > Oak Park, IL 60301 > office (708) 383-6040 X 10 > cell (708) 822-3738 > > -- " Smile .... it confuses people " ------------------------------ Message: 8 Date: Mon, 5 Apr 2010 08:08:17 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com To: 'Joe Nocito' , Andrew Burgeson , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A050@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Talk about politically incorrect. "Pregnant, out of wedlock 16 year olds?" Are you kidding me? Seriously AB? Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 01, 2010 7:42 PM To: Andrew Burgeson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com I've seen lab get accredited one month, only to have CLIA come through the next month and rip that lab apart. I have been through CAP inspections that consisted of the inspector looking only at slides while he checked off the checklist to inspectors carrying their own thermometers and testing the temp of the paraffin baths on the tissue processors. This is supposed to be a peer review, often it's a witch hunt. I had one CAP team leader tell me that my lab was the last lab he was going to inspect because he dropped out of CAP and went with JCAHO, which was approved by his hospital administrator. Does anyone know why CAP split the "processing" and "grossing parts anyway? I thought it was a stupid idea in the first place. But then again, I've never been known to be politically correct Joe ----- Original Message ----- From: "Andrew Burgeson" To: Sent: Thursday, April 01, 2010 12:26 PM Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com > Sheesh is right, J. > > CAP is all politics as far as I am concerned. It is all > about protecting the careers and paychecks of the general > pathology community. > > I am thouroughly unimpressed with JCAHO, CAP et al. > > If all you need to legally run a laboratory is to be CLIA > inspected, then WHY BOTHER with these subjective entities? > > > The BS I have heard over the last few months concerning MOHS > surgery specimens is one glaring example of the limitations > CAP has in understanding fully certain nuances of the lab > trade. > > Ridiculous. Unless you want the marketing and potential > "perception" that you are better covered from a legal > standpoint, CAP certs are worthless. > > The more I hear about CAP certifications, the more I see it > as a certain community of individuals who are protecting > their perceived "TURF." > > In the end, the pathologists in the group and in the > facility in which you are working have to take > responsibility for these matters. If the docs think a CAP > cert is necessary, then do it and live with it. If not, then > consider yourself lucky to not have to see these people in > your lab. > > I have been through MANY CAP inspections in and out of the > military. For the most part, though, I see people paying > this organization to inspect their lab as the same thing as > "burning a pinch of incense in honor of great Caesar, ruler > of Rome." It will get you some kudos, but tangibly not > change much at all if your pathologists or HR $ hiring hands > want to pocket more $ as a result of hiring "pregnant out of > wedlock 16 year olds" to gross tissue and cut slides. > > Seen it. > > AB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. 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Thank you for your cooperation. ------------------------------ Message: 9 Date: Mon, 5 Apr 2010 09:00:58 -0500 From: "Bernice Frederick" Subject: RE: [Histonet] RCC To: "'Howery, Jeffrey'" , Message-ID: <6D9B42048B2E43C4AEEC2B630377C443@lurie.northwestern.edu> Content-Type: text/plain; charset="US-ASCII" RCC from Novacastra (Leica) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey Sent: Friday, April 02, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC What are people using for a Renal cell Carcinoma. ( this is a test ) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 5 Apr 2010 10:06:17 -0400 From: "McMahon, Loralee A" Subject: [Histonet] RE: CD5 To: "Howery, Jeffrey" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" High pH Retrieval for 20 minutes in the PT module 20 minute incubation of CD5 15 minute mouse linker 20 Minute incubation Flex HRP 10 minute DAB 5 minute Hematoxylin Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 5 Apr 2010 10:26:08 -0400 From: "Parsons, Catherine" Subject: [Histonet] Choosing Carousel Style Tissue Processors To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am in the process of selecting a new tissue processor for my lab, and have narrowed the search to two models. Both are carousel style tissue processors. We are a small, low volume research lab, so the carousel style fits well with our processing and space needs. I would appreciate any information/opinions that anyone has about these two models and manufacturers. Major lab equipment purchases seem to be on a 20 year cycle here, so I need to choose carefully and well! My choices are the Leica TP 1020 and the Thermo Scientific Microm STP 120 Spin tissue processor. Has anyone found the spin function on the Microm unit to be useful, or just another part to break down and need replacement? Thanks in advance for any information that you can offer. Cathy Parsons cparsons@vt.edu ------------------------------ Message: 12 Date: Mon, 5 Apr 2010 09:55:30 -0500 From: "Dawson, Glen" Subject: [Histonet] RE: CD5 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Jeff, Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue# RM-9119-S. Concentration of 1:75 diluted w/Dako diluent. Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down. 35 minute primary antibody application. 30 minute Envision+ polyclonal (DAKO) secondary antibody detection. 7 minute DAB+ (DAKO). Counter-stain as you like. Results on this antibody are excellent. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 5 *************************************** From cfrmd1 <@t> gmail.com Mon Apr 5 13:22:29 2010 From: cfrmd1 <@t> gmail.com (Carlos Rodriguez, MD) Date: Mon Apr 5 13:22:35 2010 Subject: [Histonet] Sakura E300 TP Message-ID: Hi All For those of you who use or have used a Sakura E300 TP, what has your experience and overall impression been? I'm particularly interested in input from anyone who has worked with a refurbished E300, including how well it has held up (or not) compared to more recent model TPs. Any maintenance issues, malfunction problems, etc that stand out? I would be using it for a skin specimens only. Thanks very much!! Have a great day Carlos From 41dmb41 <@t> gmail.com Mon Apr 5 13:37:46 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Apr 5 13:38:09 2010 Subject: [Histonet] Amyloid Stain Message-ID: Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew From LSebree <@t> uwhealth.org Mon Apr 5 14:05:23 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Apr 5 14:05:30 2010 Subject: [Histonet] Amyloid Stain In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF81F@UWHC-MAIL01.uwhis.hosp.wisc.edu> Drew, At one time we were going to bring this stain in-house (Amyloid A) but finding positive control material was problematic. One of our pathologists remembered a case from more than 20 years ago (and had documented it!) but it was still just a tiny renal biopsy. We found that ProPath (214-237-1894, www.propath.com ) does this antibody so we gave them some of our renal biopsy and since then have sent our cases to them for staining. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Monday, April 05, 2010 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid Stain Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshea121 <@t> roadrunner.com Mon Apr 5 14:13:54 2010 From: jshea121 <@t> roadrunner.com (Shea's) Date: Mon Apr 5 14:14:01 2010 Subject: [Histonet] number of slides Message-ID: 1. Most bxs we cut 2 levels (one level/slide, no more than 2 sections/slide). 2. Prostate bx are unique. Our Urologists love us and send color coded bxs in 2 containers (Left & Right, yellow = apex, green = midgland, red= transition, blue = base). We then are able to place all bxs in a total of 2 cassettes instead of 8 if submitted separately. We still charge cpt code 88305 x 8 because each specimen is identified and there are still 8 separate diagnosis on the report, but it saves a lot of time, and slides as you can imagine. For these we will cut about 3 long ribbons and pick up serial sections labeled 1 - 10. We H&E stain level 1, 5 & 10 and save all others for PIN 4 if needed. 3. Only on bxs that tend to get requests for immunos/special stains (cx bx for p16 & k-67 or GIs for H. Pylori), we will cut 6-10 slides. Stain the first, last & middle, then save all others for immunos, for good pt care. It may sound like a waste of time/slides, but we do not want to cut through to level 3, then find out that an immuno is needed on level 1. Unlike other labs, our pathologists do not want to see a slide with more than 2 serial sections on a it, from the same level (that is just redundant). They also prefer 2 slides for 2 levels and 3 slides for 3 levels (just in case one is sent out or broken). Our Pathologists definitely have the final decision as to how they want their slides, after all, it is their name on the report. We all work very well together, so we discuss what works best for the good of the patient, pathologists and techs, then keep it consistent for all Pathologists. It gets too crazy when each Pathologist wants slides a different way. It will be interesting to see what others are doing. Jan From KPierce <@t> cancer-test.com Mon Apr 5 14:18:15 2010 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Mon Apr 5 14:19:24 2010 Subject: [Histonet] Histo position in Seattle Wa. Message-ID: <9380B79A09A6DD43884D977256FC12020F5355@fleming.mla.local> Med Lab Assoc. in Seattle Wa. is looking for a full time tech with experience in cutting, embedding. special stains and IHC. We are a growing lab and plan to expand into new areas of pathology/molecluar biology as well as our association with a highly regarded Clinical lab. Position is Mom-Fri., 6 AM to 2:30 PM. If interested contact me at kpierce@cancer-test.com or at 206-623-3814 between 10 AM and 5 PM Pacific daylight savings time. Ken Pierce. lab supervisor From sbreeden <@t> nmda.nmsu.edu Mon Apr 5 14:22:16 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Apr 5 14:22:22 2010 Subject: [Histonet] Histo Stories, Part 2 Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46FEE@nmdamailsvr.nmda.ad.nmsu.edu> PLEASE send requests for the histo stories to my HOME EMAIL ADDRESS - not via Histonet!!! nmhisto@comcast.net. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From llewllew <@t> shaw.ca Mon Apr 5 14:37:26 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Apr 5 14:37:32 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: References: <4bba17d6.38a.2b93.692087890@siscom.net> Message-ID: <514007B0CBEB48EF861A10069B37D7EF@BryanPC> Comparing the situations in different countries can be very confusing. I traied in the UK (many years ago) and have lived in Canada for a long time, but I do have some (limited) information about the US system. First off, Medical Laboratory Technology in the UK and Canada includes histotechnology as one of the integral subject areas, but the US does not. Histotechnology is a standalone subject there, by and large. The ASCP is different from the Health Professional Council (used to be Council for professions supplementary to medicine when I lived there). That is a licensing body, and its function is carried out by some state agencies (in the US) and some provincial agencies (in Canada). However, not all states and provinces require licensing to work as a medical laboratory technologist/histotechnologist. The ASCP used to run the commonest US qualifying exams (still do ??, I am not sure) and kept a registry of qualified technologists, although there are others systems. In Canada it is done by the CSMLS (Canadian Society for Medical Laboratory Sciences). The equivalent organisation in the UK is the IBMS (Institute of Biomedical Sciences). In Canada it is possible to take specialty training in a subject area at both initial level and post initial level. So you can be an RT (Registered Technologist) in medical laboratory technology generally, or an RT in cytology or electron microscopy as examples. All RTs can take advanced examinations as general or specialist technologists, depending on their initial RT status. There used to be a third level (Fellowship in the CSMLS) but it was abandoned because so few technologists took it. That was about the same level as the UK three part exam. An applicable BSc is now required in Canada to advance post RT. As to 16 year olds in labs. I started work in the UK in Hackney six days before my 17th birthday in 1960. A month later I was doing haemoglobins by finger stick with Hagedorn needles, ESRs and going around the wards. A year later I was well versed in clinical chemistry (urea, glucose, bilirubin, alkaline phosphatases etc - all done manually with what passed for micro methods in those days. Students like me did about 80% of the work in those days because the profession was expanding so fast due to the introduction of the public health care system in Britain. Things change, and that just would not be allowed today. I suspect that 16 year olds doing grossing is very unusal and in the US would likely be viewed as an invitation for the pathologists to be sued. Remember, it was April 1st! In the US the CAP (College of American Pathologists) is involved in an accreditation system with other agencies. In Canada the CAP (Canadian Association of Pathologists) is a player in some accrediatation systems, but in Canada health care is legally a provincial responsibility, so accreditation is done by provincial agencies for each province. That is also the reason licensing varies from province to province here. Our country wide qualifying system by the CSMLS is a fortunate anomoly that nobody wants to change because it works so well for us. I hope this explains a little. Bryan Llewellyn ----- Original Message ----- From: "Malika Benatti" To: "Mark Tarango" Cc: ; "Andrew Burgeson" Sent: Monday, April 05, 2010 11:03 AM Subject: Re: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com >I am very confuse reading every email reply to this tread also I would be > really grateful if someone could enlighten with regard to what is the > comment practice in the US. > > Having been trained as a histotechnologist although we are call Specialist > Biomedical Scientist in the UK, we cannot practice unless we are fully > registered with the Health Professional Council HPC, which I believe has > the > same role as the ASCP. Every 2 years we may be audited a demonstrate that > we > fully comply with HPC regulation and CPD or lose or registration. All > laboratories are accredited by the Clinical Pathology Accreditation CPA > under the international organization for standardization legislation (ISO > 15189). > > Laboratory accreditation happen every 2 years cycle for which the > laboratory > has to comply with a set of standard. > During inspection accessor review everything with a fine tooth comb, and > score you some of the issues may just be minors but they will always get > you > with a critical issue, which you will have a set amount of time to > correct, > they will then return and verify that all non compliance and critical > issues > have been address before giving you CPA accreditation status. > > Having a 16 years old out of school with little experience in histology > and > no formal training grossing specimen is never heard off, only Register > Biomedical Scientist are allowed to do small biopsies, Advance > Practitioner, > Trainee Pathologist, will be involved in the grossing of lager specimens, > and tumour specimens. > > > > > On Mon, Apr 5, 2010 at 6:18 PM, Mark Tarango > wrote: > >> When I was 16 years-old I was grossing in the lab. We had >> very busy pathologists (busy reading slides) who thought it was okay to >> train their courier (me at the time) to gross. I had already been there >> handing them bottles and closing cassette lids for several months. >> >> When we had our first CLIA inspection, they had me intial and sign >> paperwork >> saying that I had grossed so many cases of various specimen types under >> patholgist supervision and had been grossing so long. The problem was >> that >> I was only a high school graduate at the time. They then changed >> direction >> and told the inspectors that the pathologists did all the grossing. >> >> Just brings back memories. Thought I'd share. >> >> Mark Tarango >> On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson >> wrote: >> >> > The point is not about gender, as I stated before... >> > >> > It's about a person's health risks and lack of training >> > overlooked for the sake of labor. TYVM >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > " Smile .... it confuses people " > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From malbenatti <@t> googlemail.com Mon Apr 5 14:58:12 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Mon Apr 5 14:58:22 2010 Subject: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com In-Reply-To: <514007B0CBEB48EF861A10069B37D7EF@BryanPC> References: <4bba17d6.38a.2b93.692087890@siscom.net> <514007B0CBEB48EF861A10069B37D7EF@BryanPC> Message-ID: Bryan, Thanks for your details explanation on what look to be a very different system. Though something I am confused with an maybe you or someone else can clarify this for me. In the UK to be allow to practice as a Biomedical Scientist in any laboratory discipline, you need of have a Accredited Hons Degree, and undergo your HPC registration, Specialist status is only acquire after passing the part 2 of the HPC registration (this is pretty new ). Most of fully qualify Specialist Biomedical Scientist have also Post Graduate qualifications. Now I am reading right that not all the histotechnologist in the US are ASCP registered ? Is the ASCP registration not a Mendatory requirement to practice as a histotechnologist ? Does the registration rules vary from states to states? Cheers Malika On Mon, Apr 5, 2010 at 8:37 PM, Bryan Llewellyn wrote: > Comparing the situations in different countries can be very confusing. I > traied in the UK (many years ago) and have lived in Canada for a long time, > but I do have some (limited) information about the US system. > > First off, Medical Laboratory Technology in the UK and Canada includes > histotechnology as one of the integral subject areas, but the US does not. > Histotechnology is a standalone subject there, by and large. > > The ASCP is different from the Health Professional Council (used to be > Council for professions supplementary to medicine when I lived there). That > is a licensing body, and its function is carried out by some state agencies > (in the US) and some provincial agencies (in Canada). However, not all > states and provinces require licensing to work as a medical laboratory > technologist/histotechnologist. The ASCP used to run the commonest US > qualifying exams (still do ??, I am not sure) and kept a registry of > qualified technologists, although there are others systems. In Canada it is > done by the CSMLS (Canadian Society for Medical Laboratory Sciences). The > equivalent organisation in the UK is the IBMS (Institute of Biomedical > Sciences). > > In Canada it is possible to take specialty training in a subject area at > both initial level and post initial level. So you can be an RT (Registered > Technologist) in medical laboratory technology generally, or an RT in > cytology or electron microscopy as examples. All RTs can take advanced > examinations as general or specialist technologists, depending on their > initial RT status. There used to be a third level (Fellowship in the CSMLS) > but it was abandoned because so few technologists took it. That was about > the same level as the UK three part exam. An applicable BSc is now required > in Canada to advance post RT. > > As to 16 year olds in labs. I started work in the UK in Hackney six days > before my 17th birthday in 1960. A month later I was doing haemoglobins by > finger stick with Hagedorn needles, ESRs and going around the wards. A year > later I was well versed in clinical chemistry (urea, glucose, bilirubin, > alkaline phosphatases etc - all done manually with what passed for micro > methods in those days. Students like me did about 80% of the work in those > days because the profession was expanding so fast due to the introduction of > the public health care system in Britain. Things change, and that just > would not be allowed today. I suspect that 16 year olds doing grossing is > very unusal and in the US would likely be viewed as an invitation for the > pathologists to be sued. Remember, it was April 1st! > > In the US the CAP (College of American Pathologists) is involved in an > accreditation system with other agencies. In Canada the CAP (Canadian > Association of Pathologists) is a player in some accrediatation systems, but > in Canada health care is legally a provincial responsibility, so > accreditation is done by provincial agencies for each province. That is > also the reason licensing varies from province to province here. Our > country wide qualifying system by the CSMLS is a fortunate anomoly that > nobody wants to change because it works so well for us. > > I hope this explains a little. > > Bryan Llewellyn > > ----- Original Message ----- From: "Malika Benatti" < > malbenatti@googlemail.com> > To: "Mark Tarango" > Cc: ; "Andrew Burgeson" < > napoli@siscom.net> > Sent: Monday, April 05, 2010 11:03 AM > Subject: Re: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com > > > I am very confuse reading every email reply to this tread also I would be >> really grateful if someone could enlighten with regard to what is the >> comment practice in the US. >> >> Having been trained as a histotechnologist although we are call Specialist >> Biomedical Scientist in the UK, we cannot practice unless we are fully >> registered with the Health Professional Council HPC, which I believe has >> the >> same role as the ASCP. Every 2 years we may be audited a demonstrate that >> we >> fully comply with HPC regulation and CPD or lose or registration. All >> laboratories are accredited by the Clinical Pathology Accreditation CPA >> under the international organization for standardization legislation (ISO >> 15189). >> >> Laboratory accreditation happen every 2 years cycle for which the >> laboratory >> has to comply with a set of standard. >> During inspection accessor review everything with a fine tooth comb, and >> score you some of the issues may just be minors but they will always get >> you >> with a critical issue, which you will have a set amount of time to >> correct, >> they will then return and verify that all non compliance and critical >> issues >> have been address before giving you CPA accreditation status. >> >> Having a 16 years old out of school with little experience in histology >> and >> no formal training grossing specimen is never heard off, only Register >> Biomedical Scientist are allowed to do small biopsies, Advance >> Practitioner, >> Trainee Pathologist, will be involved in the grossing of lager specimens, >> and tumour specimens. >> >> >> >> >> On Mon, Apr 5, 2010 at 6:18 PM, Mark Tarango >> wrote: >> >> When I was 16 years-old I was grossing in the lab. We had >>> very busy pathologists (busy reading slides) who thought it was okay to >>> train their courier (me at the time) to gross. I had already been there >>> handing them bottles and closing cassette lids for several months. >>> >>> When we had our first CLIA inspection, they had me intial and sign >>> paperwork >>> saying that I had grossed so many cases of various specimen types under >>> patholgist supervision and had been grossing so long. The problem was >>> that >>> I was only a high school graduate at the time. They then changed >>> direction >>> and told the inspectors that the pathologists did all the grossing. >>> >>> Just brings back memories. Thought I'd share. >>> >>> Mark Tarango >>> On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson >>> wrote: >>> >>> > The point is not about gender, as I stated before... >>> > >>> > It's about a person's health risks and lack of training >>> > overlooked for the sake of labor. TYVM >>> > >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> >> -- >> " Smile .... it confuses people " >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > -- " Smile .... it confuses people " From Rcartun <@t> harthosp.org Mon Apr 5 16:12:10 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 5 16:12:22 2010 Subject: [Histonet] Amyloid Stain In-Reply-To: References: Message-ID: <4BBA19EA.7400.0077.1@harthosp.org> I find IHC for amyloid A (AA) helpful only in kidney; however, it is not requested often. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Drew Meyer <41dmb41@gmail.com> 4/5/2010 2:37 PM >>> Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greenjumpyone <@t> hotmail.com Mon Apr 5 15:43:13 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Mon Apr 5 16:16:11 2010 Subject: [Histonet] (no subject) Message-ID: http://fulavaxeg.ucoz.ua/ _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2 From dunatrsd <@t> sbcglobal.net Mon Apr 5 16:35:05 2010 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Apr 5 16:35:10 2010 Subject: [Histonet] CD34 marker for Rat tisue In-Reply-To: References: Message-ID: <23327.45887.qm@web83902.mail.sp1.yahoo.com> Does anyone have a protocol or?any information on CD34 antibody that works on FFPE rat tissue? ? Thank you Dusko Trajkovic From gankam <@t> googlemail.com Mon Apr 5 17:37:06 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Mon Apr 5 17:37:16 2010 Subject: [Histonet] iNOS/nitrotyrosine antibody In-Reply-To: <514007B0CBEB48EF861A10069B37D7EF@BryanPC> References: <4bba17d6.38a.2b93.692087890@siscom.net> <514007B0CBEB48EF861A10069B37D7EF@BryanPC> Message-ID: <0FA570F05A9A4F379EF5E65FF08AA100@PCdeGANKAM> Dear All Looking for the best possible iNOS and nitrotyrosine antibodies that work with rat brain tissue on paraffin section I used the one from chemicon in the past but results were not so great I also use the one from labvision with disappointing results. Any help ? Thanks Dr Fabrice GANKAM -----Message d'origine----- De?: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] De la part de Bryan Llewellyn Envoy??: lundi 5 avril 2010 21:37 ??: Histonet; malbenatti@gmail.com Objet?: Re: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com Comparing the situations in different countries can be very confusing. I traied in the UK (many years ago) and have lived in Canada for a long time, but I do have some (limited) information about the US system. First off, Medical Laboratory Technology in the UK and Canada includes histotechnology as one of the integral subject areas, but the US does not. Histotechnology is a standalone subject there, by and large. The ASCP is different from the Health Professional Council (used to be Council for professions supplementary to medicine when I lived there). That is a licensing body, and its function is carried out by some state agencies (in the US) and some provincial agencies (in Canada). However, not all states and provinces require licensing to work as a medical laboratory technologist/histotechnologist. The ASCP used to run the commonest US qualifying exams (still do ??, I am not sure) and kept a registry of qualified technologists, although there are others systems. In Canada it is done by the CSMLS (Canadian Society for Medical Laboratory Sciences). The equivalent organisation in the UK is the IBMS (Institute of Biomedical Sciences). In Canada it is possible to take specialty training in a subject area at both initial level and post initial level. So you can be an RT (Registered Technologist) in medical laboratory technology generally, or an RT in cytology or electron microscopy as examples. All RTs can take advanced examinations as general or specialist technologists, depending on their initial RT status. There used to be a third level (Fellowship in the CSMLS) but it was abandoned because so few technologists took it. That was about the same level as the UK three part exam. An applicable BSc is now required in Canada to advance post RT. As to 16 year olds in labs. I started work in the UK in Hackney six days before my 17th birthday in 1960. A month later I was doing haemoglobins by finger stick with Hagedorn needles, ESRs and going around the wards. A year later I was well versed in clinical chemistry (urea, glucose, bilirubin, alkaline phosphatases etc - all done manually with what passed for micro methods in those days. Students like me did about 80% of the work in those days because the profession was expanding so fast due to the introduction of the public health care system in Britain. Things change, and that just would not be allowed today. I suspect that 16 year olds doing grossing is very unusal and in the US would likely be viewed as an invitation for the pathologists to be sued. Remember, it was April 1st! In the US the CAP (College of American Pathologists) is involved in an accreditation system with other agencies. In Canada the CAP (Canadian Association of Pathologists) is a player in some accrediatation systems, but in Canada health care is legally a provincial responsibility, so accreditation is done by provincial agencies for each province. That is also the reason licensing varies from province to province here. Our country wide qualifying system by the CSMLS is a fortunate anomoly that nobody wants to change because it works so well for us. I hope this explains a little. Bryan Llewellyn ----- Original Message ----- From: "Malika Benatti" To: "Mark Tarango" Cc: ; "Andrew Burgeson" Sent: Monday, April 05, 2010 11:03 AM Subject: Re: [Histonet] 72644.18148.qm@web111105.mail.gq1.yahoo.com >I am very confuse reading every email reply to this tread also I would be > really grateful if someone could enlighten with regard to what is the > comment practice in the US. > > Having been trained as a histotechnologist although we are call Specialist > Biomedical Scientist in the UK, we cannot practice unless we are fully > registered with the Health Professional Council HPC, which I believe has > the > same role as the ASCP. Every 2 years we may be audited a demonstrate that > we > fully comply with HPC regulation and CPD or lose or registration. All > laboratories are accredited by the Clinical Pathology Accreditation CPA > under the international organization for standardization legislation (ISO > 15189). > > Laboratory accreditation happen every 2 years cycle for which the > laboratory > has to comply with a set of standard. > During inspection accessor review everything with a fine tooth comb, and > score you some of the issues may just be minors but they will always get > you > with a critical issue, which you will have a set amount of time to > correct, > they will then return and verify that all non compliance and critical > issues > have been address before giving you CPA accreditation status. > > Having a 16 years old out of school with little experience in histology > and > no formal training grossing specimen is never heard off, only Register > Biomedical Scientist are allowed to do small biopsies, Advance > Practitioner, > Trainee Pathologist, will be involved in the grossing of lager specimens, > and tumour specimens. > > > > > On Mon, Apr 5, 2010 at 6:18 PM, Mark Tarango > wrote: > >> When I was 16 years-old I was grossing in the lab. We had >> very busy pathologists (busy reading slides) who thought it was okay to >> train their courier (me at the time) to gross. I had already been there >> handing them bottles and closing cassette lids for several months. >> >> When we had our first CLIA inspection, they had me intial and sign >> paperwork >> saying that I had grossed so many cases of various specimen types under >> patholgist supervision and had been grossing so long. The problem was >> that >> I was only a high school graduate at the time. They then changed >> direction >> and told the inspectors that the pathologists did all the grossing. >> >> Just brings back memories. Thought I'd share. >> >> Mark Tarango >> On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson >> wrote: >> >> > The point is not about gender, as I stated before... >> > >> > It's about a person's health risks and lack of training >> > overlooked for the sake of labor. TYVM >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > " Smile .... it confuses people " > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Apr 5 18:23:52 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Apr 5 18:24:12 2010 Subject: [Histonet] Removal of carbon pigment In-Reply-To: <31959.202.125.145.178.1270267658.squirrel@brain.net.pk> Message-ID: Muhammad, Unfortunately you are stuck with the carbon. No technique (short of removing the cells/tissue section) will remove the carbon. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk Sent: Saturday, 3 April 2010 3:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removal of carbon pigment Hellow all. We have received CT guided FANA smears from lung .The siles are full of carbon particles that mask the under histiocytes and granulomata.W require the removal of carbor particles.Kindly help out to remove them. Muhammad Tahseen Supervisor Histology Javed uz zaman Supervisor Cytology SKMT PAKISTAN LAHORE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From rsrichmond <@t> gmail.com Mon Apr 5 18:27:25 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Apr 5 18:27:29 2010 Subject: [Histonet] Re: Amyloid Stain Message-ID: I'd interpret a request for an amyloid stain as just that - rather than immunohistochemistry. Congo red is of course the traditional dye for staining amyloid. It's long been out of use in the textile industry, and apparently is manufactured in small quantities for histologic use, and is certified by the Biological Stain Commission as an amyloid stain. I'm not certain it's still in manufacture. Holde Puchtler introduced Sirius red as a substitute for Congo red in the 1970's, and it still has some vogue. Once again it may not be in manufacture. Dick Dapson at Anatech (www.anatechltdusa.com - I have no connection with them) introduced "Amyloid Red" (Direct red 72, C.I. 29200) as an amyloid stain. He describes it as rather similar to Congo red. I haven't seen it - has anyone? You can get it from Anatech. Examination of a dye stain for amyloid requires a polarization system (and no, I don't mean a pair of broken sun glasses in the desk drawer), and if the pathologist doesn't have access to a polarizer (many of us don't) you probably ought to be sending out your amyloid stains. Finally, control material is hard to get. (The best controls I've seen in recent years have been sections of medullary thyroid carcinomas.) Supposedly undeparaffinized sections don't keep for more than a month, so that the control block may need to be recut for each occasional use, obviously an unthrifty procedure. I've asked this question several times over the years and never got an answer. It's fairly easy to produce amyloidosis in laboratory animals. Why isn't animal material available for controls? Bob Richmond Samurai Pathologist Knoxville TN From Tony_Reilly <@t> health.qld.gov.au Mon Apr 5 21:36:42 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Apr 5 21:41:18 2010 Subject: [Histonet] Cleaning VIP Processor containers In-Reply-To: References: Message-ID: <4BBB2AD9.471C.0039.0@health.qld.gov.au> Hi Brandi Do you do hot water flushes? If not the film will be a build up of the buffer salts from your formalin. To flush replace your formalin containers with hot water and write a program to run each container for 10-15 minutes. This will not only clean the retorts but the fluid lines as well. This should be done weekly or at least monthly if time is an issue. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From greenjumpyone <@t> hotmail.com Tue Apr 6 06:47:11 2010 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Tue Apr 6 06:47:16 2010 Subject: [Histonet] (no subject) Message-ID: http://opityhenog.ucoz.hu/ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From sbreeden <@t> nmda.nmsu.edu Tue Apr 6 08:14:53 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Apr 6 08:14:59 2010 Subject: [Histonet] Histo Stories - Send Yours Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46FF2@nmdamailsvr.nmda.ad.nmsu.edu> I'm typing like a madwoman and am up to about 6 pages of single-spaced stories with at least 25 more contributions to add. This is a very interesting read and I want to make sure everyone has a chance to Tell Their Tale. If you would like to add your story to the collection, please send it to me before the beginning of next week so I can meet my self-imposed deadline of getting it done and distributed to you around April 15th. PLEASE SEND TO MY HOME EMAIL: nmhisto@comcast.net - not to Histonet so we don't "clog" up the mechanism. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From donna <@t> milestonemed.com Tue Apr 6 08:01:47 2010 From: donna <@t> milestonemed.com (Donna Willis) Date: Tue Apr 6 08:34:11 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Would someone from Histonet please stop these emails. Donna Willis, HT/HTL(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Tuesday, April 06, 2010 6:47 AM To: aga8ton@live.com; histonet@lists.utsouthwestern.edu; jkbrown2986@hotmail.com Subject: [Histonet] (no subject) http://opityhenog.ucoz.hu/ _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=P ID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5__________________________ _____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Tue Apr 6 09:31:50 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Apr 6 09:33:20 2010 Subject: [Histonet] qihc Message-ID: Is there a practice test out there for the QIHC? ThanksPatholgy Supervisor Kathy Baldwin, SCT (ASCP) M [1]sbaldwin@mhhcc.org Ph& nbsp;812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. References 1. ="mailto:sbaldwin@mhhcc.org" From brett_connolly <@t> merck.com Tue Apr 6 10:02:44 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Apr 6 10:02:52 2010 Subject: [Histonet] anti-F4/80 on IntelliFlex Message-ID: <63EA0607835FBA4689CEA9EA8B48269202F20A1B@usctmx1141.merck.com> Is anyone running the Serotec rat anti-mouse F4/80 on the Biocare IntelliFlex platform? We are having background problems. Thanks, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From alisha <@t> ka-recruiting.com Tue Apr 6 10:47:28 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Apr 6 10:47:15 2010 Subject: [Histonet] High Paying Histology Job Opportunities Message-ID: <1056349876.1270568848614.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histotechs into permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? One particular client that I am working with is a financially sound, medically advanced, full-service hospital in Southern NH. My client is looking for both a HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Another client that I am working with is 215+ bed acute care facility in eastern MA. This hospital has been voted one of the top 100 hospitals in the country to work for every year for the past 7 years. My client is looking for both a Histotech certified and a Immunohistochemistry technologist for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: Histotechs/Cytotechs NY - New York City - Histotech 3rd shift NV -Las Vegas - Histotech 3rd shift NV Las Vegas - Histology Supervisor - 3rd shift PA - Cytology Supervisor CA - Palm Springs - Histotech - 1st shift CA - Southern - Histotech NH - HTL and HT GA - Atlanta - Histotech GA - Atlanta - Pathology Coordinator (HT or CT) MA - IHC Tech MA - Histotech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus. Read Below to Find Out More: Do you know of anyone who is a great histotech and may be interested in hearing about other opportunities? Would you like to make $500 if you refer a histotech to us that we place into a new job? If so, please send us names and contact information of any histotech you know...if now or in the future we place that histotech into a new job, you will receive a $500 referral bonus. If you would like to keep the referral confidential - just write that in your notes. To refer a histotech: Go to: http://www.ka-recruiting.com/ Scroll to the bottom of the page to enter in your referrals. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From KMB01 <@t> grh.org Tue Apr 6 11:33:09 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Tue Apr 6 11:33:15 2010 Subject: [Histonet] Time allotted for Frozen sections Message-ID: Good morning histoland. What is the maximum time allotted for frozen section per block? Is this written in CAP regs? JCAHO regs? Thanks, Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From rjbuesa <@t> yahoo.com Tue Apr 6 12:09:27 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 6 12:09:32 2010 Subject: [Histonet] Time allotted for Frozen sections In-Reply-To: Message-ID: <382702.38475.qm@web65704.mail.ac4.yahoo.com> CAP requires an explanation as to why any FS diagnosis takes more than 20 minutes after the sample reaches the lab. The average time is 15 minutes. Ren? J. --- On Tue, 4/6/10, Kathy M. Gorham wrote: From: Kathy M. Gorham Subject: [Histonet] Time allotted for Frozen sections To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 6, 2010, 12:33 PM Good morning histoland.? What is the maximum time allotted for frozen section per block?? Is this written in CAP regs?? JCAHO regs? Thanks, Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission.? If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Tue Apr 6 13:09:32 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Tue Apr 6 13:10:07 2010 Subject: [Histonet] Double staining of the same Primary Message-ID: <4BBB328C020000C500074CD7@mail.TSRH.ORG> Can anybody share their protocol for Rat spinal cord double immunostain both flourescent and ABC-HRP of the same species (mouse) Primary antibody. Thank you. From doakes <@t> olympicmedical.org Tue Apr 6 13:49:05 2010 From: doakes <@t> olympicmedical.org (Dawn Oakes) Date: Tue Apr 6 13:50:15 2010 Subject: [Histonet] Uranyl Nitrate Message-ID: Is there any substitutes for Uranyl Nitrate in the modified Steiner Stain? How is it being disposed of? Dawn Oakes HT Olympic Medical Center ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From pruegg <@t> ihctech.net Tue Apr 6 15:56:52 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Apr 6 15:57:36 2010 Subject: SPAM-LOW: [Histonet] qihc In-Reply-To: References: Message-ID: I do not believe so. There are some questions from the Self Assessment booklet from NSH on IHC/EM and Enzyme Histochemistry but since it has so many subjects to cover not much space left for just IHC. Your best bet is the Dako Handbook on IHC and the references listed on the IHC Resource Group website, www.ihcrg.org , join the group online from the website to access the references and the pp presentation on Taking the QIHC by Ethel Macrea, gotta be a member of NSH to join the IHCRG but it is a great resource. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, April 06, 2010 8:32 AM To: histonet Subject: SPAM-LOW: [Histonet] qihc Is there a practice test out there for the QIHC? ThanksPatholgy Supervisor Kathy Baldwin, SCT (ASCP) M=morial Hospital and Health Care Center [1]sbaldwin@mhhcc.org Ph& nbsp;812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. References 1. ="mailto:sbaldwin@mhhcc.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Apr 6 15:59:30 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Apr 6 16:00:13 2010 Subject: [Histonet] anti-F4/80 on IntelliFlex In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269202F20A1B@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B48269202F20A1B@usctmx1141.merck.com> Message-ID: <3790E77523054A24B4B53F508B3DB815@prueggihctechlt> Brett, I run F4/80 using the BC rat on ms detection (not their instrument) without getting BG issues, are you using Proteinase K digestion? I found that to work better than HIER. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 06, 2010 9:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] anti-F4/80 on IntelliFlex Is anyone running the Serotec rat anti-mouse F4/80 on the Biocare IntelliFlex platform? We are having background problems. Thanks, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 6 16:01:53 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 6 16:01:57 2010 Subject: [Histonet] Uranyl Nitrate In-Reply-To: Message-ID: <653634.88459.qm@web65707.mail.ac4.yahoo.com> Yes, 1% aq. sol. of phosphotungstic acid. Want the reference? Ren? J. --- On Tue, 4/6/10, Dawn Oakes wrote: From: Dawn Oakes Subject: [Histonet] Uranyl Nitrate To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, April 6, 2010, 2:49 PM Is there any substitutes for Uranyl Nitrate? in the modified Steiner Stain?? How is it being disposed of? Dawn Oakes HT Olympic Medical Center ------------------------------------------------------------------------- Confidentiality Notice:? This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.? Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.? If you are not the intended recipient, you have received this email in error.? If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.? Also know that Internet e-mail is not secure.? In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.???Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From disbrc <@t> shands.ufl.edu Tue Apr 6 16:05:58 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Tue Apr 6 16:06:08 2010 Subject: [Histonet] amyloid ? for Bob Richmond In-Reply-To: <629b753200279fa7@Shands.Local> References: <629b753200279fa7@Shands.Local> Message-ID: <4BBB69F6.72AC.0059.1@shands.ufl.edu> Hi, I was also told or read somewhere that insulinomas are good amyloid controls. I've never had a chance to test any blocks from these cases. What has your experience been? Thanks for your help. Carrie From tkngflght <@t> yahoo.com Tue Apr 6 16:09:08 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Apr 6 16:09:11 2010 Subject: [Histonet] TX Society meeting? Message-ID: <833406.16237.qm@web50906.mail.re2.yahoo.com> ? ?Any word on Texas' regional histology meeting?? I'm looking for dates and the registration info for my team-- ? Thanks! ? Cheryl Kerry, HT(ASCP) From Rcartun <@t> harthosp.org Tue Apr 6 16:14:33 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 6 16:14:42 2010 Subject: [Histonet] amyloid ? for Bob Richmond In-Reply-To: <4BBB69F6.72AC.0059.1@shands.ufl.edu> References: <629b753200279fa7@Shands.Local> <4BBB69F6.72AC.0059.1@shands.ufl.edu> Message-ID: <4BBB6BF8.7400.0077.1@harthosp.org> Yes, insulinomas can show amyloid; however, they are very rare tumors. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Carrie Disbrow" 4/6/2010 5:05 PM >>> Hi, I was also told or read somewhere that insulinomas are good amyloid controls. I've never had a chance to test any blocks from these cases. What has your experience been? Thanks for your help. Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Tue Apr 6 16:57:16 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Tue Apr 6 16:57:23 2010 Subject: [Histonet] Cleaning VIP Processor containers In-Reply-To: <4BBB2AD9.471C.0039.0@health.qld.gov.au> References: <4BBB2AD9.471C.0039.0@health.qld.gov.au> Message-ID: <276470.25744.qm@web113805.mail.gq1.yahoo.com> Brandi- The flush should be with warm water (you do not need hot), and should be for the fixative station(s) and the first alcohol.? You only need 1 minute in each, just enough to get the solution to pump in. Joe Saby, BA HT ________________________________ From: Anthony Reilly To: Brandi Higgins ; histonet@lists.utsouthwestern.edu Sent: Mon, April 5, 2010 10:36:42 PM Subject: Re: [Histonet] Cleaning VIP Processor containers Hi Brandi Do you do hot water flushes? If not the film will be a build up of the buffer salts from your formalin.? To flush replace your formalin containers with hot water and write a program to run each container for 10-15 minutes.? This will not only clean the retorts but the fluid lines as well.? This should be done weekly or at least monthly if time is an issue. regards Tony Tony Reilly? B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA? Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web:? www.health.qld.gov.au/qhcss/ ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited.? The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email.? You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Apr 6 17:44:46 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Apr 6 17:44:51 2010 Subject: [Histonet] Uranyl Nitrate Message-ID: Yes. Please post the reference. - John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Rene J Buesa Date: Tuesday, April 6, 2010 17:03 Subject: Re: [Histonet] Uranyl Nitrate To: "histonet@lists.utsouthwestern.edu" , Dawn Oakes > Yes, 1% aq. sol. of phosphotungstic acid. > Want the reference? > Ren? J. > > --- On Tue, 4/6/10, Dawn Oakes wrote: > > > From: Dawn Oakes > Subject: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > Date: Tuesday, April 6, > 2010, 2:49 PM > > > Is there any substitutes for Uranyl Nitrate in the modified Steiner > Stain? How is it being disposed of? > > > > Dawn Oakes HT > > Olympic Medical Center > > > ----------------------------------------------------------------- > -------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended individual(s) > named above and may contain confidential, privileged, and/or > protected information. Any unauthorized review, use, > disclosure, copying, or distribution of its contents is > prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender > immediately by reply email and delete/destroy the original and > all copies of this communication. Also know that Internet e- > mail is not secure. In choosing to communicate with Olympic > Medical Center by email you will assume these confidentiality > risks. Internet messages may become corrupted, incomplete, or > may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihiggins <@t> gmail.com Tue Apr 6 20:21:50 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Tue Apr 6 20:21:55 2010 Subject: [Histonet] Cleaning VIP Processor Containers - Summary Message-ID: Thank you to everyone who responded. I got some good suggestions which I plan on trying. Just incase the responses I have received weren't sent to everyone, and also to make an easier-to-read reference for the future - Dilute acetic acid. xylene for waxy buildup Greased Lightning cleaner (store bought) 50/50 bleach mix - let sit for a while. warm water flushes in fixative and first alcohol The only response I received before changing the solutions was the dilulte acetic acid which we tried but did not find effective. Brandi Higgins From itai.moshe <@t> mail.huji.ac.il Wed Apr 7 04:00:25 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Wed Apr 7 04:00:32 2010 Subject: [Histonet] Aniline blue Message-ID: What type of collagen aniline blue is staining ? Thanks in advance Itai From rjbuesa <@t> yahoo.com Wed Apr 7 08:35:16 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 7 08:35:20 2010 Subject: [Histonet] Uranyl Nitrate In-Reply-To: Message-ID: <160131.64085.qm@web65703.mail.ac4.yahoo.com> Reference for phosphotungstic acid substituting uranyl nitrate as a sensitizer: Journal of Histotechnology, 2001; 24(2):113-116 Ren? J. --- On Tue, 4/6/10, John Kiernan wrote: From: John Kiernan Subject: Re: [Histonet] Uranyl Nitrate To: "Rene J Buesa" Cc: "histonet@lists.utsouthwestern.edu" Date: Tuesday, April 6, 2010, 6:44 PM Yes.? Please post the reference. - ? John Kiernan ? Anatomy, UWO ? London, Canada = = = ----- Original Message ----- From: Rene J Buesa Date: Tuesday, April 6, 2010 17:03 Subject: Re: [Histonet] Uranyl Nitrate To: "histonet@lists.utsouthwestern.edu" , Dawn Oakes > Yes, 1% aq. sol. of phosphotungstic acid. > Want the reference? > Ren? J. > > --- On Tue, 4/6/10, Dawn Oakes wrote: > > > From: Dawn Oakes > Subject: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > Date: Tuesday, April 6, > 2010, 2:49 PM > > > Is there any substitutes for Uranyl Nitrate? in the modified Steiner > Stain?? How is it being disposed of? > > > > Dawn Oakes HT > > Olympic Medical Center > > > ----------------------------------------------------------------- > -------- > Confidentiality Notice:? This e-mail message, including any > attachments, is for the sole use of the intended individual(s) > named above and may contain confidential, privileged, and/or > protected information.? Any unauthorized review, use, > disclosure, copying, or distribution of its contents is > prohibited.? If you are not the intended recipient, you have > received this email in error.? If so, please notify the sender > immediately by reply email and delete/destroy the original and > all copies of this communication.? Also know that Internet e- > mail is not secure.? In choosing to communicate with Olympic > Medical Center by email you will assume these confidentiality > risks.???Internet messages may become corrupted, incomplete, or > may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ????? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From itai.moshe <@t> mail.huji.ac.il Wed Apr 7 08:45:46 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Wed Apr 7 08:45:53 2010 Subject: [Histonet] picro sirius red, sirius red connective tissue stain[Scanned] Message-ID: picro Sirius red and Fast green protocol: Sirius red (Sirius Red F3B)- 0.1% in Picric acid. Fast Green - 0.03% in Picric acid (100%). Can be re-used, filter twice before each use. 1) Deparaffinization and Rehydration: A) Xylen - 10 min X2 B) Ethanol 100% - 2 Min X2 C) Ethanol 96% - 2 Min X1 D) Ethanol 80% - 2 Min X1 E) Ethanol 70% - 2 Min X1 2) Rinse in double distilled water (DDW) - 5 Min 3) Rinse in filtered Fast green - 20 Min 4) Wash with DDW ( just fill the slide box with DDW and empty). 5) Rinse in filtered Sirius red - 5 Min. 6) Wash with DDW ( just fill the slides box with DDW and empty). 7) Check in microscope, if the colors are not strong enough, repeat from step 2 to 7. That's it. -- Itai Moshe Mark Pines lab Institute of Animal Sciences,Volcani Center. Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew University of Jerusalem Israel ------Original Message----- *Adam Bazama* baza0013 <@t> d.umn.edu *Mon Oct 6 10:27:37 CDT 2008* - Previous message: [Histonet] gastrin antibodies..... - Next message: [Histonet] out dated reagents - *Messages sorted by:* [ date ] [ thread ] [ subject ] [ author ] ------------------------------ Hi Dave, I noticed that you posted a request in march of 2004 on histonet for a Sirius red fast green staining protocol. If you do have a protocol, would you mind sending it to me. I have had a hard time finding one to compare to mine and my lack luster results. Thank you, Adam Bazama, B.S. Junior Scientist Lillehei Heart Institute Histology and Microscopy Core Facility University of Minnesota Medical School, Division of Cardiology 4-266 Nils Hasselmo Hall 312 Church Street SE Minneapolis, MN 55455 Lab Desk: 612-625-6779 Cell: 952-334-0607 -------------------------------------------- *David Rushworth* David.Rushworth <@t> mail.bhrv.nwest.nhs.uk *Thu Mar 4 09:00:18 CST 2004* - Previous message: [Histonet] reticulin procedure - Next message: [Histonet] Research labs - charges and a question about outside contracting - *Messages sorted by:* [ date ] [ thread ] [ subject ] [ author ] ------------------------------ Can someone please supply method for Sirius red-fast green stain for connective tissue. Thanks in anticipation. Dave. ------------------------------ - Previous message: [Histonet] reticulin procedure - Next message: [Histonet] Research labs - charges and a question about outside contracting - *Messages sorted by:* [ date ] [ thread ] [ subject ] [ author ] From BMolinari <@t> heart.thi.tmc.edu Wed Apr 7 09:46:20 2010 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Apr 7 09:46:24 2010 Subject: [Histonet] frozen lung sections Message-ID: Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS, then 4% paraformaldehyde then into sucrose. Can anyone clarify the procedure or offer advice? I am unfamiliar with this technique since most of my frozen sectioning is heart tissue and it does not present the same problem as the lung. Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From lyonm <@t> upstate.edu Wed Apr 7 09:58:08 2010 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Wed Apr 7 09:57:07 2010 Subject: [Histonet] Old Manuals Message-ID: <00d001cad662$bc6c6580$35453080$@edu> Found a couple of old manuals and will send them if you want them: Directions for use of 7203 Thomas-Franz Microtome Knife Sharpener Reference Manual No. 80-A Horizontal Sliding Microtome, Lipshaw Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 From Dishop.Megan <@t> tchden.org Wed Apr 7 10:33:07 2010 From: Dishop.Megan <@t> tchden.org (Dishop, Megan) Date: Wed Apr 7 10:33:21 2010 Subject: [Histonet] RE: frozen lung sections In-Reply-To: References: Message-ID: We inflate our lung tissue with a thin OCT compound (50% OCT/ 50% 0.5M sucrose mixture) using a needle and syringe and then embed the lung in 100% OCT and snap freeze. It helps for interpretation to have open alveoli and also gives the tissue some stability internally for frozen sections. Megan K. Dishop MD Department of Pathology, B120 The Children's Hospital 13123 E. 16th Avenue Aurora, CO 80045 Ph: 720-777-4337 Fax: 720-777-7119 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Wednesday, April 07, 2010 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen lung sections Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS, then 4% paraformaldehyde then into sucrose. Can anyone clarify the procedure or offer advice? I am unfamiliar with this technique since most of my frozen sectioning is heart tissue and it does not present the same problem as the lung. Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail is confidential, may be legally privileged, and for the intended recipient only. Access, disclosure, copying, forwarding and distribution by any means is strictly prohibited. If received in error, do not read but delete and e-mail confirmation to the sender. ================================================================================ From talulahgosh <@t> gmail.com Wed Apr 7 11:10:23 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Apr 7 11:10:32 2010 Subject: [Histonet] herring sperm dna Message-ID: Hello all! Roche has decided to stop manufacturing herring sperm DNA. I thought this would be an easy product to replace but apparently there's roughly ten bjillion versions of herring sperm DNA. I have no idea whether ours was sheared, sonicated, molecular biology grade, etc. It just said herring sperm DNA on the label. Any suggestions on a replacement? We are using this, btw, in hybridization buffer for RNA in situ on chick embyro sections on slides. I called Roche and asked tech services what their replacement would be, but they just said, here's another DNA product which uses the term hybridization in its protocol, COT Human DNA, read the tech sheet to see if that's a good replacement. Of course, the tech sheet says nothing about whether it would be good in in situ hybridization buffer, just that it's good for suppressing cross hybridization in DNA microarrays. The really frustrating thing is their DIG in situ hybridization protocol calls for herring sperm DNA. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea From KMB01 <@t> grh.org Wed Apr 7 11:27:47 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Wed Apr 7 11:27:54 2010 Subject: [Histonet] Time allotted for Frozen sections In-Reply-To: <382702.38475.qm@web65704.mail.ac4.yahoo.com> Message-ID: Thanks, to all you responded to my question about times for FS. CAP says 20 min. and that's what most everyone else said. Kathy ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, April 06, 2010 10:09 AM To: histonet@lists.utsouthwestern.edu; Kathy M. Gorham Subject: Re: [Histonet] Time allotted for Frozen sections CAP requires an explanation as to why any FS diagnosis takes more than 20 minutes after the sample reaches the lab. The average time is 15 minutes. Ren? J. --- On Tue, 4/6/10, Kathy M. Gorham wrote: From: Kathy M. Gorham Subject: [Histonet] Time allotted for Frozen sections To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 6, 2010, 12:33 PM Good morning histoland. What is the maximum time allotted for frozen section per block? Is this written in CAP regs? JCAHO regs? Thanks, Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Apr 7 11:45:03 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Apr 7 11:45:23 2010 Subject: [Histonet] abtibodies Message-ID: <000001cad671$abb3db60$031b9220$@com> Hello Histonetters I am currently looking to add three new antibodies, napsin, D2-40 and villin. I stain only human FFPE tissue. If anyone has used these antibodies I would appreciate your input. Thanks in advance. Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From MMargiotta <@t> bmhmc.org Wed Apr 7 11:52:07 2010 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Wed Apr 7 11:52:14 2010 Subject: [Histonet] instrument disinfection Message-ID: Hi All, I need to order disinfecting solution for our grossing instruments. I would like to know what product most labs are using? Thanks for the info. Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Albert.Santiago <@t> uphs.upenn.edu Wed Apr 7 11:56:55 2010 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Apr 7 11:57:22 2010 Subject: [Histonet] (no subject) Message-ID: Hello fellow histonetters, we're in the process of researching bar coding/specimen tracking system for our lab and I was wondering if anyone had any recommendation of any particular system and/or vendor that I should look into. Thank you Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-662-6150-fax The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Janice.Mahoney <@t> alegent.org Wed Apr 7 12:21:29 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Apr 7 12:21:53 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A075@EXCHMBC2.ad.ah.local> In my opinion Ventana's Vantage is miles ahead of anyone else. We've had the system for over a year and love it. My techs would not want to work without it. It is much more than a tracking system, you will be amazed at the information Vantage can give you. Very user friendly and takes very little space. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert Sent: Wednesday, April 07, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello fellow histonetters, we're in the process of researching bar coding/specimen tracking system for our lab and I was wondering if anyone had any recommendation of any particular system and/or vendor that I should look into. Thank you Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-662-6150-fax Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From tahseen <@t> brain.net.pk Wed Apr 7 12:27:27 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Wed Apr 7 12:27:35 2010 Subject: [Histonet] instrument disinfection In-Reply-To: References: Message-ID: <5686.202.125.145.178.1270661247.squirrel@brain.net.pk> We are useing 10% Virkon for gross room instrument. Muhammad Tahseen SKMCH&RC > Hi All, > > I need to order disinfecting solution for our grossing instruments. I > would like to know what product most labs are using? > Thanks for the info. > > > Michele Margiotta > BMHMC > Histology Supervisor > 631-654-7192 > > > > DISCLAIMER: > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they are > addressed. This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or the > person responsible for delivering the e-mail to the intended recipient, be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. If you have received this e-mail in error, please immediately > notify the sender via return e-mail or call Brookhaven Memorial Hospital > Medical Center at (631) 654-7282. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From collette2 <@t> mail.llnl.gov Wed Apr 7 12:25:47 2010 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Wed Apr 7 12:28:01 2010 Subject: [Histonet] herring sperm dna In-Reply-To: References: Message-ID: Hi, Emily, We use Salmon Testes DNA for our hyb buffer for whole mount RNA in situ on mouse and frog embryos, Sigma catalog # D-9156, comes in 5 x 1ml (11.0 mg/ml) tubes. It says "for hybridization" on the package ;) Hope that helps. Sincerely, Nicole Collette Lawrence Livermore National Lab/UC Berkeley At 12:10 PM -0400 4/7/10, Emily Sours wrote: >Hello all! > >Roche has decided to stop manufacturing herring sperm DNA. I thought this >would be an easy product to replace but apparently there's roughly ten >bjillion versions of herring sperm DNA. I have no idea whether ours was >sheared, sonicated, molecular biology grade, etc. It just said herring >sperm DNA on the label. Any suggestions on a replacement? We are using >this, btw, in hybridization buffer for RNA in situ on chick embyro sections >on slides. >I called Roche and asked tech services what their replacement would be, but >they just said, here's another DNA product which uses the term hybridization >in its protocol, COT Human DNA, read the tech sheet to see if that's a good >replacement. Of course, the tech sheet says nothing about whether it would >be good in in situ hybridization buffer, just that it's good for suppressing >cross hybridization in DNA microarrays. The really frustrating thing is >their DIG in situ hybridization protocol calls for herring sperm DNA. > >Emily > >Shall we always be content with the ancient tinned salad of the subsidized >novel? Or the tired ice-cream of poems which cry themselves to sleep in the >refrigerators of the mind? >-Lawrence Durrell, Clea >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://*lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Apr 7 12:32:34 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Apr 7 12:32:43 2010 Subject: [Histonet] (no subject) In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A075@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A075@EXCHMBC2.ad.ah.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C61F4@EXCHANGECLUSTER.yumaregional.local> I have to agree Vantage is an excellent system, just make sure that other basis are covered, interfacing, future instrumentation costs and also bi directional interfacing with LIS. These are costly -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, April 07, 2010 10:21 AM To: 'Santiago, Albert'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) In my opinion Ventana's Vantage is miles ahead of anyone else. We've had the system for over a year and love it. My techs would not want to work without it. It is much more than a tracking system, you will be amazed at the information Vantage can give you. Very user friendly and takes very little space. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert Sent: Wednesday, April 07, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello fellow histonetters, we're in the process of researching bar coding/specimen tracking system for our lab and I was wondering if anyone had any recommendation of any particular system and/or vendor that I should look into. Thank you Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-662-6150-fax Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From bliven.laura <@t> marshfieldclinic.org Wed Apr 7 12:42:52 2010 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Wed Apr 7 12:42:59 2010 Subject: [Histonet] Thermo Slide Mate Message-ID: <201004071742.o37Hgsvs026416@spamfilt> We have purchased the Thermo Slide Mate. Has anyone had problems in using charged slides with this system? We have not started using it, as we must validate the system first. It is always good to get feedback from others. Thanks, Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From cscampbe <@t> uci.edu Wed Apr 7 12:48:17 2010 From: cscampbe <@t> uci.edu (cscampbe@uci.edu) Date: Wed Apr 7 12:48:24 2010 Subject: [Histonet] Refixing with Bouin's for Masson's Trichrome Message-ID: <40cd416f3b546f27bae1f5391c09e4d3.squirrel@webmail.uci.edu> Hi Histonet, I am currently refixing my slides in Bouin's solution. Should I be refixing the entire tissue sample? What I mean is, should I deparrafinize my heart samples, refix in Bouin's, and reinfiltrate them before slicing with the microtome? Or is it more effective to just refix the slides with the heart sections on them? Also, my procedure for the Trichrome stain has me put the slides into phosphomolybdic acid for 5 minutes. I find that the slides come out of the procedure very dark and purple, instead of vibrant red as expected. Is the acid wash the problem? Should I reduce the time spent in the acid? Thanks! -Colin From sbaldwin <@t> mhhcc.org Wed Apr 7 12:56:59 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Apr 7 12:58:26 2010 Subject: [Histonet] (no subject) Message-ID: Hi Histonet We use D2 40 podoplanin, control Lymph Node the ventana Benchmark LT, I can share this pr like! Thanks Patholgy Supervis Kathy Baldwin, SCT (ASCP) Memorial Hospital&nb [1]sbaldwin@mhhcc.org Ph 812-482-0210, Pager 812-481-089 Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhc From Vickroy.Jim <@t> mhsil.com Wed Apr 7 13:17:46 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Apr 7 13:17:52 2010 Subject: [Histonet] BREAST IMPLANTS AND HARDWARE Message-ID: <24A4826E8EF0964D86BC5317306F58A542583FF7BD@mmc-mail.ad.mhsil.com> Our pathology group charges a "gross only" charge for hardware and implants removed from surgery that go to the pathology department. Our current policy states that normally these devices do not have to be sent to pathology however when the material is requested by the patient to be returned to the patient or the patient's clinician it must go to the pathology department for accessioning and documentation. Since we do a gross description and work with the proper paperwork for release of the items we currently charge an 88300 charge which is a gross only charge. Our plastics department questions the necessity of this position and why we are charging for it. Can anybody else share how they are handling these types of materials removed at surgery? In addition our risk management team tells us that these devices have to have a release signed by the patient before they can be given to the patient or the patient's surgeon. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From gu.lang <@t> gmx.at Wed Apr 7 13:22:05 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 7 13:22:13 2010 Subject: AW: [Histonet] Refixing with Bouin's for Masson's Trichrome In-Reply-To: <40cd416f3b546f27bae1f5391c09e4d3.squirrel@webmail.uci.edu> References: <40cd416f3b546f27bae1f5391c09e4d3.squirrel@webmail.uci.edu> Message-ID: <881CE4C688CF4E59BE8EF75671C216AE@dielangs.at> Refixing the slides is a usual procedure. RT over night or 2 hours 60?C oven. The longer the acid step the brighter the red stain. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von cscampbe@uci.edu Gesendet: Mittwoch, 07. April 2010 19:48 An: HistoNet Betreff: [Histonet] Refixing with Bouin's for Masson's Trichrome Hi Histonet, I am currently refixing my slides in Bouin's solution. Should I be refixing the entire tissue sample? What I mean is, should I deparrafinize my heart samples, refix in Bouin's, and reinfiltrate them before slicing with the microtome? Or is it more effective to just refix the slides with the heart sections on them? Also, my procedure for the Trichrome stain has me put the slides into phosphomolybdic acid for 5 minutes. I find that the slides come out of the procedure very dark and purple, instead of vibrant red as expected. Is the acid wash the problem? Should I reduce the time spent in the acid? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 7 15:25:43 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 7 15:25:47 2010 Subject: [Histonet] Refixing with Bouin's for Masson's Trichrome In-Reply-To: <40cd416f3b546f27bae1f5391c09e4d3.squirrel@webmail.uci.edu> Message-ID: <612088.44436.qm@web65705.mail.ac4.yahoo.com> No, you should not. What you call "refixing" is just a mordant step before Masson's trichrome and you can do that ito every section you need to stain. ? As a matter of fact the whole tissue sample will not be "refixed" with Bouin's, and you could lose tissue orientation and other conditions now present in your sections. Ren? J. --- On Wed, 4/7/10, cscampbe@uci.edu wrote: From: cscampbe@uci.edu Subject: [Histonet] Refixing with Bouin's for Masson's Trichrome To: "HistoNet" Date: Wednesday, April 7, 2010, 1:48 PM Hi Histonet, I am currently refixing my slides in Bouin's solution. Should I be refixing the entire tissue sample? What I mean is, should I deparrafinize my heart samples, refix in Bouin's, and reinfiltrate them before slicing with the microtome? Or is it more effective to just refix the slides with the heart sections on them? Also, my procedure for the Trichrome stain has me put the slides into phosphomolybdic acid for 5 minutes. I find that the slides come out of the procedure very dark and purple, instead of vibrant red as expected. Is the acid wash the problem? Should I reduce the time spent in the acid? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Wed Apr 7 15:37:40 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Apr 7 15:37:51 2010 Subject: [Histonet] Biocare Universal Negative Control Serum may be positive Message-ID: Having just validated and started using Biocare Universal Negative Control Serum, we were surprised when a pathologist brought back two cases in which tumour and/or epithelium came up positive. This staining was in the same locations as positive staining with some markers. We use Ventana BenchMark XT's, and the staining was in both iView and UltraView protocols. Mechanical problems, contamination and dilution of the Negative Control Serum did not appear to be factors. Biocare knows about this and is meeting with Ventana to iron out the problem. Would have been nice to know about this if it was a known issue before we bought and started using the product, but better late than never. Posting this in case anyone else on Histonet is experiencing similar staining challenges using this product. As Biocare states in the product insert sheet, when used in conjunction with non-Biocare kits, results may vary. We'll keep using it and are currently waiting for advice from Biocare. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From pathmaster <@t> yahoo.com Wed Apr 7 15:40:17 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Wed Apr 7 15:40:21 2010 Subject: [Histonet] Cleaning the gunk off grossing instruments Message-ID: <400497.12621.qm@web111111.mail.gq1.yahoo.com> Hi Michelle, I use dilute bleach, no specific dilution, usually 10-20%, melts blood and grime away. I always make a fresh bucket each day? and keep it in the sink to rinse the instruments and store them when I'm away from the grossing bench. Don't leave them overnight though, some forceps will corrode at the welded point. But then again, I'm not using formalin- I use Histochoice for 95% of my specimens and formalin for the other 5%. Formalin and bleach fumes are not to be mixed since a potent carcinogen, bis-chloromethyl ether,? can be formed. Just keep the formalin away from the bleach. If you want, I'll drop off a gallon on my way to work tomorrow :-).? It's the least I can do to repay your letting me cut those kidney sections for my QIHC all those years ago. Jeff Silverman- just trying to be neighborly From SDrew <@t> uwhealth.org Wed Apr 7 15:54:25 2010 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Wed Apr 7 15:54:31 2010 Subject: [Histonet] Biocare Universal Negative Control Serum may be positive In-Reply-To: References: Message-ID: <738A7878143FF74BB77436E255743C1A010004E4@UWHC-MAIL03.uwhis.hosp.wisc.edu> We've been using that product for ages on our Ventana instruments and not had a problem. I wonder if it is batch related? We're expecting a new lot-guess we'll be taking an even closer look than usual at our testing slides! Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, April 07, 2010 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Universal Negative Control Serum may be positive Having just validated and started using Biocare Universal Negative Control Serum, we were surprised when a pathologist brought back two cases in which tumour and/or epithelium came up positive. This staining was in the same locations as positive staining with some markers. We use Ventana BenchMark XT's, and the staining was in both iView and UltraView protocols. Mechanical problems, contamination and dilution of the Negative Control Serum did not appear to be factors. Biocare knows about this and is meeting with Ventana to iron out the problem. Would have been nice to know about this if it was a known issue before we bought and started using the product, but better late than never. Posting this in case anyone else on Histonet is experiencing similar staining challenges using this product. As Biocare states in the product insert sheet, when used in conjunction with non-Biocare kits, results may vary. We'll keep using it and are currently waiting for advice from Biocare. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Apr 7 15:59:14 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 7 15:59:18 2010 Subject: [Histonet] Biocare Universal Negative Control Serum may be positive In-Reply-To: References: Message-ID: We use Biocare's Universal Negative Control Serum for our negative controls on the Benchmark XT. I qc the IHC slides daily and don't see any staining in the negative sections. We are a large reference lab and I see lots of negative control slides daily. You might have gotten a bad batch. I've had much more trouble with Ventana's negative rabbit serum before they finally discontinued it and switched to a new product. Mark Tarango On Wed, Apr 7, 2010 at 1:37 PM, Gagnon, Eric wrote: > Having just validated and started using Biocare Universal Negative Control > Serum, we were surprised when a pathologist brought back two cases in which > tumour and/or epithelium came up positive. This staining was in the same > locations as positive staining with some markers. We use Ventana BenchMark > XT's, and the staining was in both iView and UltraView protocols. > Mechanical problems, contamination and dilution of the Negative Control > Serum did not appear to be factors. > > Biocare knows about this and is meeting with Ventana to iron out the > problem. Would have been nice to know about this if it was a known issue > before we bought and started using the product, but better late than never. > Posting this in case anyone else on Histonet is experiencing similar > staining challenges using this product. As Biocare states in the product > insert sheet, when used in conjunction with non-Biocare kits, results may > vary. We'll keep using it and are currently waiting for advice from > Biocare. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Wed Apr 7 16:05:33 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 7 16:05:39 2010 Subject: [Histonet] Mast Cell Staining using Ultraview Kit from Ventana Message-ID: Hi Histonetters, I remember a while back someone asking about this. I just wanted to post for anyone having trouble with Ventana's ultraview kit (mast cell staining) that when you use a combination of CC1 and protease, the mast cell staining goes away. Thanks Mark Tarango From marktarango <@t> gmail.com Wed Apr 7 16:07:51 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 7 16:07:56 2010 Subject: [Histonet] Re: Mast Cell Staining using Ultraview Kit from Ventana In-Reply-To: References: Message-ID: I forgot to add: I was talking about pankeratin staining in sentinal nodes. On Wed, Apr 7, 2010 at 2:05 PM, Mark Tarango wrote: > Hi Histonetters, > > I remember a while back someone asking about this. I just wanted to post > for anyone having trouble with Ventana's ultraview kit (mast cell staining) > that when you use a combination of CC1 and protease, the mast cell staining > goes away. > > Thanks > > Mark Tarango > From sfeher <@t> CMC-NH.ORG Wed Apr 7 16:13:54 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Apr 7 16:14:05 2010 Subject: [Histonet] TX Society meeting? In-Reply-To: <833406.16237.qm@web50906.mail.re2.yahoo.com> References: <833406.16237.qm@web50906.mail.re2.yahoo.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B74F6E@exchange.cmc-nh.org> Contact KDWYER3322@AOL.COM. April 23-25, Houston, TX. Be sure to sign up for the "Designing a LEAN Pathology Lab from Scratch" workshop on Saturday, April 24. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, April 06, 2010 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TX Society meeting? ? ?Any word on Texas' regional histology meeting?? I'm looking for dates and the registration info for my team-- ? Thanks! ? Cheryl Kerry, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Apr 7 17:13:40 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Apr 7 17:13:47 2010 Subject: [Histonet] Thermo Slide Mate Message-ID: <57BE698966D5C54EAE8612E8941D7683086D4EDF@EXCHANGE3.huntingtonhospital.com> We use both charged slides and regular slides regularly with no problems. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bliven, Laura Sent: Wednesday, April 07, 2010 10:43 AM To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [Histonet] Thermo Slide Mate We have purchased the Thermo Slide Mate. Has anyone had problems in using charged slides with this system? We have not started using it, as we must validate the system first. It is always good to get feedback from others. Thanks, Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From dhill32 <@t> comcast.net Wed Apr 7 19:28:58 2010 From: dhill32 <@t> comcast.net (David Hill) Date: Wed Apr 7 19:28:15 2010 Subject: [Histonet] Re: Uranyl Nitrate Message-ID: <5E0FDE4E82804F9FB8BD4088412AB405@DavidPC> We use Protocol zinc formalin and it works great. Our steiners look better than they ever had with uranyl nitrate. It's much cheaper also. David Hill B.S. CT(ASCP)HTL,QIHC Assistant Histology Supervisor Trumbull Labs Germantown, TN ----- Original Message ----- From: To: Sent: Wednesday, April 07, 2010 12:02 PM Subject: Histonet Digest, Vol 77, Issue 8 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Time allotted for Frozen sections (Rene J Buesa) > 2. Double staining of the same Primary (Reuel Cornelia) > 3. Uranyl Nitrate (Dawn Oakes) > 4. RE: SPAM-LOW: [Histonet] qihc (Patsy Ruegg) > 5. RE: anti-F4/80 on IntelliFlex (Patsy Ruegg) > 6. Re: Uranyl Nitrate (Rene J Buesa) > 7. amyloid ? for Bob Richmond (Carrie Disbrow) > 8. TX Society meeting? (Cheryl) > 9. Re: amyloid ? for Bob Richmond (Richard Cartun) > 10. Re: Cleaning VIP Processor containers (Joseph Saby) > 11. Re: Uranyl Nitrate (John Kiernan) > 12. Cleaning VIP Processor Containers - Summary (Brandi Higgins) > 13. Aniline blue (Itai Moshe) > 14. Re: Uranyl Nitrate (Rene J Buesa) > 15. picro sirius red, sirius red connective tissue > stain[Scanned] (Itai Moshe) > 16. frozen lung sections (Molinari, Betsy) > 17. Old Manuals (Michael J. Lyon, Ph.D.) > 18. RE: frozen lung sections (Dishop, Megan) > 19. herring sperm dna (Emily Sours) > 20. RE: Time allotted for Frozen sections (Kathy M. Gorham) > 21. abtibodies (Cynthia Pyse) > 22. instrument disinfection (Margiotta, Michele) > 23. (no subject) (Santiago, Albert) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 6 Apr 2010 10:09:27 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Time allotted for Frozen sections > To: histonet@lists.utsouthwestern.edu, "Kathy M. Gorham" > > Message-ID: <382702.38475.qm@web65704.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > CAP requires an explanation as to why any FS diagnosis takes more than 20 > minutes after the sample reaches the lab. > The average time is 15 minutes. > Ren? J. > > --- On Tue, 4/6/10, Kathy M. Gorham wrote: > > > From: Kathy M. Gorham > Subject: [Histonet] Time allotted for Frozen sections > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, April 6, 2010, 12:33 PM > > > Good morning histoland. What is the maximum time allotted for frozen > section per block? Is this written in CAP regs? JCAHO regs? > Thanks, Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > > GRH Mission > We will ensure ACCESS to superior QUALITY, affordable health care for all > those in need of our services, including underserved populations within > our communities. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is > protected > by Federal and State Law. Federal regulations prohibit further > distribution or > copying of this information without permission. If you received this > e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 06 Apr 2010 13:09:32 -0500 > From: "Reuel Cornelia" > Subject: [Histonet] Double staining of the same Primary > To: > Message-ID: <4BBB328C020000C500074CD7@mail.TSRH.ORG> > Content-Type: text/plain; charset=US-ASCII > > Can anybody share their protocol for Rat spinal cord double immunostain > both flourescent and ABC-HRP of the same species (mouse) Primary antibody. > Thank you. > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 6 Apr 2010 11:49:05 -0700 > From: Dawn Oakes > Subject: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Is there any substitutes for Uranyl Nitrate in the modified Steiner > Stain? How is it being disposed of? > > > > Dawn Oakes HT > > Olympic Medical Center > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended individual(s) named above and may > contain confidential, privileged, and/or protected information. Any > unauthorized review, use, disclosure, copying, or distribution of its > contents is prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender immediately > by reply email and delete/destroy the original and all copies of this > communication. Also know that Internet e-mail is not secure. In choosing > to communicate with Olympic Medical Center by email you will assume these > confidentiality risks. Internet messages may become corrupted, > incomplete, or may incorrectly identify the sender. > > > ------------------------------ > > Message: 4 > Date: Tue, 6 Apr 2010 14:56:52 -0600 > From: "Patsy Ruegg" > Subject: RE: SPAM-LOW: [Histonet] qihc > To: "'Sara Baldwin/mhhcc.org'" , "'histonet'" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > I do not believe so. There are some questions from the Self Assessment > booklet from NSH on IHC/EM and Enzyme Histochemistry but since it has so > many subjects to cover not much space left for just IHC. Your best bet is > the Dako Handbook on IHC and the references listed on the IHC Resource > Group > website, www.ihcrg.org , join the group online from the website to access > the references and the pp presentation on Taking the QIHC by Ethel Macrea, > gotta be a member of NSH to join the IHCRG but it is a great resource. > > Regards, > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara > Baldwin/mhhcc.org > Sent: Tuesday, April 06, 2010 8:32 AM > To: histonet > Subject: SPAM-LOW: [Histonet] qihc > > > Is there a practice test out there for the QIHC? > ThanksPatholgy Supervisor > Kathy Baldwin, SCT (ASCP) > M=morial Hospital and Health Care Center > [1]sbaldwin@mhhcc.org > Ph& nbsp;812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 > > Confidential information, Authorized use only. > > References > > 1. ="mailto:sbaldwin@mhhcc.org" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 6 Apr 2010 14:59:30 -0600 > From: "Patsy Ruegg" > Subject: RE: [Histonet] anti-F4/80 on IntelliFlex > To: "'Connolly, Brett M'" , > > Message-ID: <3790E77523054A24B4B53F508B3DB815@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > Brett, > > I run F4/80 using the BC rat on ms detection (not their instrument) > without > getting BG issues, are you using Proteinase K digestion? I found that to > work better than HIER. > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, > Brett M > Sent: Tuesday, April 06, 2010 9:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] anti-F4/80 on IntelliFlex > > Is anyone running the Serotec rat anti-mouse F4/80 on the Biocare > IntelliFlex platform? We are having background problems. > > Thanks, > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at http://www.merck.com/contact/contacts.html) > that > may be confidential, proprietary copyrighted and/or legally privileged. It > is intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 6 Apr 2010 14:01:53 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > , Dawn Oakes > > Message-ID: <653634.88459.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Yes, 1% aq. sol. of phosphotungstic acid. > Want the reference? > Ren? J. > > --- On Tue, 4/6/10, Dawn Oakes wrote: > > > From: Dawn Oakes > Subject: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > > Date: Tuesday, April 6, 2010, 2:49 PM > > > Is there any substitutes for Uranyl Nitrate in the modified Steiner > Stain? How is it being disposed of? > > > > Dawn Oakes HT > > Olympic Medical Center > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended individual(s) named above and may contain > confidential, privileged, and/or protected information. Any unauthorized > review, use, disclosure, copying, or distribution of its contents is > prohibited. If you are not the intended recipient, you have received this > email in error. If so, please notify the sender immediately by reply email > and delete/destroy the original and all copies of this communication. Also > know that Internet e-mail is not secure. In choosing to communicate with > Olympic Medical Center by email you will assume these confidentiality > risks. Internet messages may become corrupted, incomplete, or may > incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 06 Apr 2010 17:05:58 -0400 > From: "Carrie Disbrow" > Subject: [Histonet] amyloid ? for Bob Richmond > To: > Message-ID: <4BBB69F6.72AC.0059.1@shands.ufl.edu> > Content-Type: text/plain; charset=US-ASCII > > > > Hi, > I was also told or read somewhere that insulinomas are good amyloid > controls. I've never had a chance to test any blocks from these cases. > What has your experience been? Thanks for your help. > Carrie > > > ------------------------------ > > Message: 8 > Date: Tue, 6 Apr 2010 14:09:08 -0700 (PDT) > From: Cheryl > Subject: [Histonet] TX Society meeting? > To: histonet@lists.utsouthwestern.edu > Message-ID: <833406.16237.qm@web50906.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > > Any word on Texas' regional histology meeting? I'm looking for dates and > the registration info for my team-- > > Thanks! > > Cheryl Kerry, HT(ASCP) > > > ------------------------------ > > Message: 9 > Date: Tue, 06 Apr 2010 17:14:33 -0400 > From: "Richard Cartun" > Subject: Re: [Histonet] amyloid ? for Bob Richmond > To: , "Carrie Disbrow" > > Message-ID: <4BBB6BF8.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Yes, insulinomas can show amyloid; however, they are very rare tumors. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Carrie Disbrow" 4/6/2010 5:05 PM >>> > > > Hi, > I was also told or read somewhere that insulinomas are good amyloid > controls. I've never had a chance to test any blocks from these cases. > What has your experience been? Thanks for your help. > Carrie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Tue, 6 Apr 2010 14:57:16 -0700 (PDT) > From: Joseph Saby > Subject: Re: [Histonet] Cleaning VIP Processor containers > To: Anthony Reilly , Brandi Higgins > , histonet@lists.utsouthwestern.edu > Message-ID: <276470.25744.qm@web113805.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Brandi- > > The flush should be with warm water (you do not need hot), and should be > for the fixative station(s) and the first alcohol. You only need 1 minute > in each, just enough to get the solution to pump in. > > Joe Saby, BA HT > > > > > ________________________________ > From: Anthony Reilly > To: Brandi Higgins ; > histonet@lists.utsouthwestern.edu > Sent: Mon, April 5, 2010 10:36:42 PM > Subject: Re: [Histonet] Cleaning VIP Processor containers > > Hi Brandi > > Do you do hot water flushes? If not the film will be a build up of the > buffer salts from your formalin. To flush replace your formalin containers > with hot water and write a program to run each container for 10-15 > minutes. This will not only clean the retorts but the fluid lines as well. > This should be done weekly or at least monthly if time is an issue. > > regards > Tony > > > > > > Tony Reilly B.Sc. , M.Sc. > Chief Scientist, Anatomical Pathology > Pathology Queensland-PA Laboratory > _________________________________________________ > Clinical and Statewide Services Division| QueenslandHealth > > Level 1, Building 15,Princess Alexandra Hospital > Ipswich Road,WOOLLOONGABBA Qld4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > Fax: 07 3176 2930 > Email: tony_reilly@health.qld.gov.au > Web: www.health.qld.gov.au/qhcss/ > > > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and > for the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended > recipient(s), or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of > this email is strictly prohibited. The information contained in this > email, including any attachment sent with it, may be subject to a > statutory duty of confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this > email in error, you are asked to immediately notify the sender by > telephone collect on Australia +61 1800 198 175 or by return email. You > should also delete this email, and any copies, from your computer system > network and destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute > or take any action(s) that relies on it; any form of disclosure, > modification, distribution and/or publication of this email is also > prohibited. > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or code that may > occur as a consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the > sender and not the views of the Queensland Government. > ********************************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Tue, 06 Apr 2010 18:44:46 -0400 > From: John Kiernan > Subject: Re: [Histonet] Uranyl Nitrate > To: Rene J Buesa > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=iso-8859-1 > > Yes. Please post the reference. > - > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Rene J Buesa > Date: Tuesday, April 6, 2010 17:03 > Subject: Re: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > , Dawn Oakes > > >> Yes, 1% aq. sol. of phosphotungstic acid. >> Want the reference? >> Ren? J. >> >> --- On Tue, 4/6/10, Dawn Oakes wrote: >> >> >> From: Dawn Oakes >> Subject: [Histonet] Uranyl Nitrate >> To: "histonet@lists.utsouthwestern.edu" >> Date: Tuesday, April 6, >> 2010, 2:49 PM >> >> >> Is there any substitutes for Uranyl Nitrate in the modified Steiner >> Stain? How is it being disposed of? >> >> >> >> Dawn Oakes HT >> >> Olympic Medical Center >> >> >> ----------------------------------------------------------------- >> -------- >> Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the intended individual(s) >> named above and may contain confidential, privileged, and/or >> protected information. Any unauthorized review, use, >> disclosure, copying, or distribution of its contents is >> prohibited. If you are not the intended recipient, you have >> received this email in error. If so, please notify the sender >> immediately by reply email and delete/destroy the original and >> all copies of this communication. Also know that Internet e- >> mail is not secure. In choosing to communicate with Olympic >> Medical Center by email you will assume these confidentiality >> risks. Internet messages may become corrupted, incomplete, or >> may incorrectly identify the sender. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 12 > Date: Tue, 6 Apr 2010 21:21:50 -0400 > From: Brandi Higgins > Subject: [Histonet] Cleaning VIP Processor Containers - Summary > To: histonet@lists.utsouthwestern.edu, Katelin Lester > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Thank you to everyone who responded. I got some good suggestions which I > plan on trying. > > Just incase the responses I have received weren't sent to everyone, and > also > to make an easier-to-read reference for the future - > Dilute acetic acid. > xylene for waxy buildup > Greased Lightning cleaner (store bought) > 50/50 bleach mix - let sit for a while. > warm water flushes in fixative and first alcohol > > The only response I received before changing the solutions was the dilulte > acetic acid which we tried but did not find effective. > > Brandi Higgins > > > ------------------------------ > > Message: 13 > Date: Wed, 7 Apr 2010 12:00:25 +0300 > From: Itai Moshe > Subject: [Histonet] Aniline blue > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > What type of collagen aniline blue is staining ? > > Thanks in advance > > Itai > > > ------------------------------ > > Message: 14 > Date: Wed, 7 Apr 2010 06:35:16 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Uranyl Nitrate > To: John Kiernan > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: <160131.64085.qm@web65703.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Reference for phosphotungstic acid substituting uranyl nitrate as a > sensitizer: > Journal of Histotechnology, 2001; 24(2):113-116 > Ren? J. > > --- On Tue, 4/6/10, John Kiernan wrote: > > > From: John Kiernan > Subject: Re: [Histonet] Uranyl Nitrate > To: "Rene J Buesa" > Cc: "histonet@lists.utsouthwestern.edu" > > Date: Tuesday, April 6, 2010, 6:44 PM > > > > Yes. Please post the reference. > - > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Rene J Buesa > Date: Tuesday, April 6, 2010 17:03 > Subject: Re: [Histonet] Uranyl Nitrate > To: "histonet@lists.utsouthwestern.edu" > , Dawn Oakes > > >> Yes, 1% aq. sol. of phosphotungstic acid. >> Want the reference? >> Ren? J. >> >> --- On Tue, 4/6/10, Dawn Oakes wrote: >> >> >> From: Dawn Oakes >> Subject: [Histonet] Uranyl Nitrate >> To: "histonet@lists.utsouthwestern.edu" >> Date: Tuesday, April 6, >> 2010, 2:49 PM >> >> >> Is there any substitutes for Uranyl Nitrate in the modified Steiner >> Stain? How is it being disposed of? >> >> >> >> Dawn Oakes HT >> >> Olympic Medical Center >> >> >> ----------------------------------------------------------------- >> -------- >> Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the intended individual(s) >> named above and may contain confidential, privileged, and/or >> protected information. Any unauthorized review, use, >> disclosure, copying, or distribution of its contents is >> prohibited. If you are not the intended recipient, you have >> received this email in error. If so, please notify the sender >> immediately by reply email and delete/destroy the original and >> all copies of this communication. Also know that Internet e- >> mail is not secure. In choosing to communicate with Olympic >> Medical Center by email you will assume these confidentiality >> risks. Internet messages may become corrupted, incomplete, or >> may incorrectly identify the sender. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 15 > Date: Wed, 7 Apr 2010 16:45:46 +0300 > From: Itai Moshe > Subject: [Histonet] picro sirius red, sirius red connective tissue > stain[Scanned] > To: histonet@lists.utsouthwestern.edu, baza0013@d.umn.edu, > David.Rushworth@mail.bhrv.nwest.nhs.uk > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > picro Sirius red and Fast green protocol: > > Sirius red (Sirius Red F3B)- 0.1% in Picric acid. > Fast Green - 0.03% in Picric acid (100%). > Can be re-used, filter twice before each use. > > > 1) Deparaffinization and Rehydration: > A) Xylen - 10 min X2 > B) Ethanol 100% - 2 Min X2 > C) Ethanol 96% - 2 Min X1 > D) Ethanol 80% - 2 Min X1 > E) Ethanol 70% - 2 Min X1 > > 2) Rinse in double distilled water (DDW) - 5 Min > > 3) Rinse in filtered Fast green - 20 Min > > 4) Wash with DDW ( just fill the slide box with DDW and empty). > > 5) Rinse in filtered Sirius red - 5 Min. > > 6) Wash with DDW ( just fill the slides box with DDW and empty). > > 7) Check in microscope, if the colors are not strong enough, repeat from > step 2 to 7. > > That's it. > > -- > Itai Moshe > Mark Pines lab > Institute of Animal Sciences,Volcani Center. > Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew > University of Jerusalem > Israel > > ------Original Message----- > *Adam Bazama* baza0013 <@t> d.umn.edu > > *Mon Oct 6 10:27:37 CDT 2008* > > > - Previous message: [Histonet] gastrin > antibodies..... > - Next message: [Histonet] out dated > reagents > - *Messages sorted by:* [ date > ] > [ > thread ] > [ > subject ] > [ > author ] > > ------------------------------ > > Hi Dave, > > > > I noticed that you posted a request in march of 2004 on histonet for a > Sirius red fast green staining protocol. If you do have a protocol, would > you mind sending it to me. I have had a hard time finding one to compare > to > mine and my lack luster results. > > > > Thank you, > > > > > > Adam Bazama, B.S. > > Junior Scientist > > Lillehei Heart Institute Histology and Microscopy Core Facility > > University of Minnesota Medical School, Division of Cardiology > 4-266 Nils Hasselmo Hall > 312 Church Street SE > Minneapolis, MN 55455 > Lab Desk: 612-625-6779 > Cell: 952-334-0607 > > -------------------------------------------- > > *David Rushworth* David.Rushworth <@t> mail.bhrv.nwest.nhs.uk > > *Thu Mar 4 09:00:18 CST 2004* > > > - Previous message: [Histonet] reticulin procedure > > - Next message: [Histonet] Research labs - charges and a question > about outside contracting > > - *Messages sorted by:* [ date ] > > [ thread ] > > [ subject ] > > [ author ] > > > ------------------------------ > > Can someone please supply method for Sirius red-fast green stain for > connective tissue. > Thanks in anticipation. > Dave. > > > > ------------------------------ > > > - Previous message: [Histonet] reticulin > procedure > - Next message: [Histonet] Research labs - charges and a question about > outside > contracting > - *Messages sorted by:* [ date > ] > [ > thread ] > [ > subject ] > [ > author ] > > > ------------------------------ > > Message: 16 > Date: Wed, 7 Apr 2010 09:46:20 -0500 > From: "Molinari, Betsy" > Subject: [Histonet] frozen lung sections > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > I have received some lung samples on OCT. These lungs have not been in > PBS or any fixative. They went directly from dissection into OCT and > frozen. > > I cannot get a section. The temp of the cryostat is -20. I have done > some reading and it seems the best results come from immersion in PBS, > then 4% paraformaldehyde then into sucrose. Can anyone clarify the > procedure or offer advice? I am unfamiliar with this technique since > most of my frozen sectioning is heart tissue and it does not present the > same problem as the lung. > > Thanks, > > Betsy > > > > Betsy Molinari HT(ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave > > MC1-283 > > Houston,TX 77030-2607 > > 832-355-6524 > > 832-355-6812 > > > > > > ------------------------------ > > Message: 17 > Date: Wed, 7 Apr 2010 10:58:08 -0400 > From: "Michael J. Lyon, Ph.D." > Subject: [Histonet] Old Manuals > To: > Message-ID: <00d001cad662$bc6c6580$35453080$@edu> > Content-Type: text/plain; charset="us-ascii" > > Found a couple of old manuals and will send them if you want them: > > > > Directions for use of 7203 Thomas-Franz Microtome Knife Sharpener > > Reference Manual No. 80-A Horizontal Sliding Microtome, Lipshaw > > > > Michael J. Lyon, Ph.D. > > Otolaryngology Research Lab > > SUNY Upstate Medical University > > 750 East Adams Street > > Syracuse, NY 13210 > > > > Voice 315-464-7253 > > Fax 315-464-5572 > > > > ------------------------------ > > Message: 18 > Date: Wed, 7 Apr 2010 09:33:07 -0600 > From: "Dishop, Megan" > Subject: [Histonet] RE: frozen lung sections > To: "Molinari, Betsy" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > We inflate our lung tissue with a thin OCT compound (50% OCT/ 50% 0.5M > sucrose mixture) using a needle and syringe and then embed the lung in > 100% OCT and snap freeze. It helps for interpretation to have open > alveoli and also gives the tissue some stability internally for frozen > sections. > > Megan K. Dishop MD > Department of Pathology, B120 > The Children's Hospital > 13123 E. 16th Avenue > Aurora, CO 80045 > Ph: 720-777-4337 > Fax: 720-777-7119 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, > Betsy > Sent: Wednesday, April 07, 2010 8:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] frozen lung sections > > Hi all, > > I have received some lung samples on OCT. These lungs have not been in > PBS or any fixative. They went directly from dissection into OCT and > frozen. > > I cannot get a section. The temp of the cryostat is -20. I have done > some reading and it seems the best results come from immersion in PBS, > then 4% paraformaldehyde then into sucrose. Can anyone clarify the > procedure or offer advice? I am unfamiliar with this technique since > most of my frozen sectioning is heart tissue and it does not present the > same problem as the lung. > > Thanks, > > Betsy > > > > Betsy Molinari HT(ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave > > MC1-283 > > Houston,TX 77030-2607 > > 832-355-6524 > > 832-355-6812 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail is confidential, may be legally > privileged, > and for the intended recipient only. Access, disclosure, copying, > forwarding and > distribution by any means is strictly prohibited. If received in error, > do not read but delete and e-mail confirmation to the sender. > ================================================================================ > > > > > ------------------------------ > > Message: 19 > Date: Wed, 7 Apr 2010 12:10:23 -0400 > From: Emily Sours > Subject: [Histonet] herring sperm dna > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hello all! > > Roche has decided to stop manufacturing herring sperm DNA. I thought this > would be an easy product to replace but apparently there's roughly ten > bjillion versions of herring sperm DNA. I have no idea whether ours was > sheared, sonicated, molecular biology grade, etc. It just said herring > sperm DNA on the label. Any suggestions on a replacement? We are using > this, btw, in hybridization buffer for RNA in situ on chick embyro > sections > on slides. > I called Roche and asked tech services what their replacement would be, > but > they just said, here's another DNA product which uses the term > hybridization > in its protocol, COT Human DNA, read the tech sheet to see if that's a > good > replacement. Of course, the tech sheet says nothing about whether it > would > be good in in situ hybridization buffer, just that it's good for > suppressing > cross hybridization in DNA microarrays. The really frustrating thing is > their DIG in situ hybridization protocol calls for herring sperm DNA. > > Emily > > Shall we always be content with the ancient tinned salad of the subsidized > novel? Or the tired ice-cream of poems which cry themselves to sleep in > the > refrigerators of the mind? > -Lawrence Durrell, Clea > > > ------------------------------ > > Message: 20 > Date: Wed, 7 Apr 2010 09:27:47 -0700 > From: "Kathy M. Gorham" > Subject: RE: [Histonet] Time allotted for Frozen sections > To: "Rene J Buesa" , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Thanks, to all you responded to my question about times for FS. CAP says > 20 min. and that's what most everyone else said. Kathy > > ________________________________ > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Tuesday, April 06, 2010 10:09 AM > To: histonet@lists.utsouthwestern.edu; Kathy M. Gorham > Subject: Re: [Histonet] Time allotted for Frozen sections > > > CAP requires an explanation as to why any FS diagnosis takes more than 20 > minutes after the sample reaches the lab. > The average time is 15 minutes. > Ren? J. > > --- On Tue, 4/6/10, Kathy M. Gorham wrote: > > > > From: Kathy M. Gorham > Subject: [Histonet] Time allotted for Frozen sections > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, April 6, 2010, 12:33 PM > > > Good morning histoland. What is the maximum time allotted for frozen > section per block? Is this written in CAP regs? JCAHO regs? > Thanks, Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > > GRH Mission > We will ensure ACCESS to superior QUALITY, affordable health care for all > those in need of our services, including underserved populations within > our communities. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is > protected > by Federal and State Law. Federal regulations prohibit further > distribution or > copying of this information without permission. If you received this > e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 21 > Date: Wed, 7 Apr 2010 12:45:03 -0400 > From: "Cynthia Pyse" > Subject: [Histonet] abtibodies > To: "'Histonet'" > Message-ID: <000001cad671$abb3db60$031b9220$@com> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters > > I am currently looking to add three new antibodies, napsin, D2-40 and > villin. I stain only human FFPE tissue. If anyone has used these > antibodies > I would appreciate your input. Thanks in advance. > > Regards > > > > Cindy Pyse, CLT, HT (ASCP) > > Histology Supervisor > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > > > ------------------------------ > > Message: 22 > Date: Wed, 7 Apr 2010 12:52:07 -0400 > From: "Margiotta, Michele" > Subject: [Histonet] instrument disinfection > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi All, > > I need to order disinfecting solution for our grossing instruments. I > would like to know what product most labs are using? > Thanks for the info. > > > Michele Margiotta > BMHMC > Histology Supervisor > 631-654-7192 > > > > DISCLAIMER: > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they are > addressed. This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or the > person responsible for delivering the e-mail to the intended recipient, be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. If you have received this e-mail in error, please immediately > notify the sender via return e-mail or call Brookhaven Memorial Hospital > Medical Center at (631) 654-7282. > > > > ------------------------------ > > Message: 23 > Date: Wed, 7 Apr 2010 12:56:55 -0400 > From: "Santiago, Albert" > Subject: [Histonet] (no subject) > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello fellow histonetters, we're in the process of researching bar > coding/specimen tracking system for our lab and I was wondering if > anyone had any recommendation of any particular system and/or vendor > that I should look into. Thank you > > Albert Santiago, HT(ASCP) > Laboratory Manager > Dermatopathology > 215-662-6759/6539-office > 215-662-6150-fax > > > > The information contained in this e-mail message is intended only for the > personal and confidential use of the recipient(s) named above. If the > reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 77, Issue 8 > *************************************** From AnthonyH <@t> chw.edu.au Wed Apr 7 20:16:31 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 7 20:16:51 2010 Subject: [Histonet] frozen lung sections In-Reply-To: Message-ID: Betsy, I would dissolve the OCT in Hanks solution (or similar cell culture medium - not saline). Place in dilute OCT (50/50 with distilled water), place on stirrer until the lung settles in the OCT 30-60min. Blot with absorbent paper, refreeze in fresh OCT. It should cut quite well. I have used this previously on some tissue that was received the same way. It seems that the OCT had "freeze-dried" prior to receipt in my lab. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, 8 April 2010 12:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen lung sections Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS, then 4% paraformaldehyde then into sucrose. Can anyone clarify the procedure or offer advice? I am unfamiliar with this technique since most of my frozen sectioning is heart tissue and it does not present the same problem as the lung. Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From annigyg <@t> gmail.com Wed Apr 7 23:11:50 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Apr 7 23:11:56 2010 Subject: [Histonet] BREAST IMPLANTS AND HARDWARE In-Reply-To: <24A4826E8EF0964D86BC5317306F58A542583FF7BD@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A542583FF7BD@mmc-mail.ad.mhsil.com> Message-ID: your path dept is being 'used' as a tracking system for these items - the charge is perfectly justifiable - we do it all the time tell the plastics guys to set up their own tracking system for these samples - less hassle for the lab!!! everything removed from a patient comes to my lab - except forskins from infants and certain debridements we have many 88300 charges regarding the release to be signed - from a medico-legal point of view it would be best to get someone to sign and get a copy of SS number (or some sort of ID) just in case my 2 cents worth AbuDhabiAnnie On 7 April 2010 22:17, Vickroy, Jim wrote: > Our pathology group charges a "gross only" charge for hardware and implants > removed from surgery that go to the pathology department. Our current policy > states that normally these devices do not have to be sent to pathology > however when the material is requested by the patient to be returned to the > patient or the patient's clinician it must go to the pathology department > for accessioning and documentation. Since we do a gross description and > work with the proper paperwork for release of the items we currently charge > an 88300 charge which is a gross only charge. Our plastics department > questions the necessity of this position and why we are charging for it. > Can anybody else share how they are handling these types of materials > removed at surgery? In addition our risk management team tells us that > these devices have to have a release signed by the patient before they can > be given to the patient or the patient's surgeon. > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From Kimberly.Marshall <@t> ahss.org Thu Apr 8 06:02:45 2010 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Thu Apr 8 06:03:00 2010 Subject: [Histonet] Traveling Histo Tech Message-ID: <136EE5CD3735EE4BB86FE88F4B6D0172D30C3D08@LKMVXCHMB22.ADVENTISTCORP.NET> Hello all, A few years back I went on vacation and we used a traveling Histo tech to cover. Back then I thought it was a neat idea and said to myself, when the kids are grown and out of the house I am gonna do that. Well that time has come, so before I started making calls and researching it I wanted to ask my fellow Histo folks what they know about it. Is there still a market for traveling techs? Any information about this would sure be appreciated. Thanks in advance Kimberly Marshall HT(ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From NSEARCY <@t> swmail.sw.org Thu Apr 8 06:10:46 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Apr 8 06:11:02 2010 Subject: [Histonet] TX Society meeting? In-Reply-To: <73A7ED895EE0C24D9267ED814911DF1912B74F6E@exchange.cmc-nh.org> References: <833406.16237.qm@web50906.mail.re2.yahoo.com> <73A7ED895EE0C24D9267ED814911DF1912B74F6E@exchange.cmc-nh.org> Message-ID: <4BBD7365.5D38.00EF.0@swmail.sw.org> Website has all of the information Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Feher, Stephen" 4/7/2010 4:13 PM >>> Contact KDWYER3322@AOL.COM. April 23-25, Houston, TX. Be sure to sign up for the "Designing a LEAN Pathology Lab from Scratch" workshop on Saturday, April 24. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, April 06, 2010 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TX Society meeting? Any word on Texas' regional histology meeting? I'm looking for dates and the registration info for my team-- Thanks! Cheryl Kerry, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From jcolclefa <@t> aol.com Thu Apr 8 08:05:38 2010 From: jcolclefa <@t> aol.com (John PJ Coleman) Date: Thu Apr 8 08:33:59 2010 Subject: [Histonet] trich/bouin's/FS/Inst cleaning/mast cells Message-ID: <90AB433A-AD51-4D0D-9CCC-DE8EE9E50B34@aol.com> Trichrome: Our Masson Trichrome procedure uses a combined phosphotungstic/phosphomolybdic acid step for 20 minutes @ RT without a rinse after, our smooth muscle stays RED.. Bouin's: Working in a rat research lab for years, we fixed all tissue in bouins for 24 hours, rinsed in water for about an hour and stored in 70% alcohol till processing. Is all your tissue post fixed in bouins or just selected items? Why not Bouin's as a primary fixative? We got great results on PTAH, Trichrome, Luxol Fast Blue stains. Frozens: CAP says monitor FS times, if 90% are not within 20 minutes, document the evaluation of why. ANP.11820 Instrument cleaning: We use TBQ which is virucidal and does not rust instruments like bleach does. Mast cells: We have not had unintentional mast cell staining on our fleet of Ultras using the either Ultraview or the alk phos kits. John PJ Coleman, HT(ASCP)QIHC From JWeems <@t> sjha.org Thu Apr 8 08:54:02 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Apr 8 08:54:09 2010 Subject: [Histonet] eProtocols Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015D4256F6@CHEXCMS10.one.ads.che.org> Hello Everyone, Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS? Thanks much!, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From bernardgerard <@t> yahoo.com Thu Apr 8 09:59:13 2010 From: bernardgerard <@t> yahoo.com (Bernard Martin) Date: Thu Apr 8 09:59:17 2010 Subject: [Histonet] Sestrin 2 Message-ID: <226491.17228.qm@web37901.mail.mud.yahoo.com> Hi all, I asked this a couple weeks ago, but thought I'd give it one more try- has anyone had any experience with Sestrin 2 for IHC? I can't find anything in the literature, and thought maybe some has tried it. There are ones available, but they all use the same picture, and I am concerned perhaps it doesn't work well. Funds are limited so we would like to get one that someone has had some success with. Thanks for any info you could provide! Bern From DKBoyd <@t> chs.net Thu Apr 8 10:05:47 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Apr 8 10:05:58 2010 Subject: [Histonet] eProtocols In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164015D4256F6@CHEXCMS10.one.ads.che.org> Message-ID: I have printed off the CAP worksheets off the web site. We use them as work sheets for the pathologist. He checks off and fills in what is pertinent to the case, then the transcriptionist transcribes the Synoptic Report into the final report directly under the Final Diagnosis. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/08/2010 09:54 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] eProtocols Hello Everyone, Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS? Thanks much!, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From cmiller <@t> physlab.com Thu Apr 8 10:17:09 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 8 10:17:16 2010 Subject: [Histonet] RE: Traveling Histo Tech In-Reply-To: <136EE5CD3735EE4BB86FE88F4B6D0172D30C3D08@LKMVXCHMB22.ADVENTISTCORP.NET> References: <136EE5CD3735EE4BB86FE88F4B6D0172D30C3D08@LKMVXCHMB22.ADVENTISTCORP.NET> Message-ID: Only to say I am envious, good luck and have fun! Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From ree3 <@t> leicester.ac.uk Thu Apr 8 10:33:01 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Apr 8 10:35:15 2010 Subject: [Histonet] RE: Travelling Histo Tech In-Reply-To: References: <136EE5CD3735EE4BB86FE88F4B6D0172D30C3D08@LKMVXCHMB22.ADVENTISTCORP.NET> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CFFB6D32@EXC-MBX3.cfs.le.ac.uk> So you could sum it up thus:- "Have knife, will slice for a price". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: 08 April 2010 16:17 To: Marshall, Kimberly; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Traveling Histo Tech Only to say I am envious, good luck and have fun! Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.thotakura <@t> imperial.ac.uk Thu Apr 8 10:38:03 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Thu Apr 8 10:38:13 2010 Subject: [Histonet] Message-ID: Hi All, I have a problem with immunohistochmesitry. I am have raised my own antibody and it is not commercially available. This monoclonal antibody works fine for western blot. When it come to immunohistochemistry on mice liver frozen section, it is binding every where. I can see staining in my control as well. Any suggestion or guess's from you guys? Anil From gu.lang <@t> gmx.at Thu Apr 8 10:38:40 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Apr 8 10:38:47 2010 Subject: [Histonet] CD 25 protocol on Benchmark Message-ID: Hi Benchmark-users! Can someone of you provide me with a protocol for CD 25 for FFPE tissue. We have purchased the antibody from Cell marque and have found nuclear staining in trephine bone marrow biopsy with mastocytosis. This specimen was decalcified with formic acid. Used protocol: CC1 mild, 1:10 for 40 min I would be happy about any help. Gudrun Lang From julia.m.hough <@t> hotmail.co.uk Thu Apr 8 11:27:26 2010 From: julia.m.hough <@t> hotmail.co.uk (julia hough) Date: Thu Apr 8 11:27:31 2010 Subject: [Histonet] CD138 IHC Message-ID: Dear all. Does anyone have a protocol for the immunohistochemistry staining of CD138 on paraffin embedded mouse tibia, including the fixation and decalcification protocol? Thanks BW Julia _________________________________________________________________ http://clk.atdmt.com/UKM/go/197222280/direct/01/ Do you have a story that started on Hotmail? Tell us now From bill501 <@t> mindspring.com Thu Apr 8 11:30:58 2010 From: bill501 <@t> mindspring.com (Bill) Date: Thu Apr 8 11:31:04 2010 Subject: [Histonet] eProtocols In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164015D4256F6@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164015D4256F6@CHEXCMS10.one.ads.che.org> Message-ID: At 9:54 AM -0400 4/8/10, Weems, Joyce wrote: >Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS?< We export data from our LIS and pick it up in Word merge templates. We then use autotext to add the appropriate protocol info (modified) for the diagnosis. Bill From MLafrini <@t> csmlab.com Thu Apr 8 11:47:14 2010 From: MLafrini <@t> csmlab.com (Michael LaFriniere) Date: Thu Apr 8 11:50:36 2010 Subject: [Histonet] Just three months????? In-Reply-To: References: <72644.18148.qm@web111105.mail.gq1.yahoo.com> Message-ID: <4BBDD046.588C.00AF.0@csmlab.com> FYI The Grandfather clause still stands as it always had.... Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 4/1/2010 at 8:46:10 PM, "Joe Nocito" wrote: I see that now, wasn't the first time I was wrong today, probably wouldn't be the last either ----- Original Message ----- From: "Jeffrey Silverman" To: Sent: Wednesday, March 31, 2010 5:03 PM Subject: [Histonet] Just three months????? CAP is now saying no more gross processing of small things that are entirely submitted- it's all gross examination now whether we mean straining currettings into a cassette or dissecting a complex cancer resection. Dumb as all get out if you ask me. As for the three month training thing, Joe, I'm not so sure about that. They seem to spell out specific amounts of college education required IN ADDITION to training in the laboratory. The requirement first spells out two education choices- 1: an earned associates degree in medical laboratory science OR 2:Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education MUST (caps mine) include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. So in addition to all the college courses, which we all know you need to count and measure six endoscopic biopsies and put them in a cassette, much more training and experience needed than what an unregistered on the job tech needs to orient and embed them properly LOL!!! CLIA then requires that the individual must have (additional) laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. Now there are grandfathering clauses in CLIA which may enable folks (like myself) to continue to gross. I haven't digested that yet, but I'm ready to get that job in Pathmark if necessary. Sheesh, does it ever end? Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mark.hocking <@t> ventana.roche.com Thu Apr 8 12:29:00 2010 From: mark.hocking <@t> ventana.roche.com (Hocking, Mark) Date: Thu Apr 8 12:29:10 2010 Subject: [Histonet] Re: Histonet Digest, Vol 77, Issue 10 Message-ID: <3C53ACB95F5C2B48B7C0A00FCFCE91B80144093190@rnumsem703.nala.roche.com> D Mark Hocking Symphony Technical Applications Specialist Ventana Medical Systems, Inc. A member of the Roche group Message sent via blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu Apr 08 13:03:49 2010 Subject: Histonet Digest, Vol 77, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: frozen lung sections (Tony Henwood) 2. Re: BREAST IMPLANTS AND HARDWARE (Anne van Binsbergen) 3. Traveling Histo Tech (Marshall, Kimberly) 4. RE: TX Society meeting? (Nita Searcy) 5. trich/bouin's/FS/Inst cleaning/mast cells (John PJ Coleman) 6. eProtocols (Weems, Joyce) 7. Sestrin 2 (Bernard Martin) 8. Re: eProtocols (DKBoyd@chs.net) 9. RE: Traveling Histo Tech (Cheri Miller) 10. RE: Travelling Histo Tech (Edwards, Richard E.) 11. (Thotakura, Anil Kumar) 12. CD 25 protocol on Benchmark (Gudrun Lang) 13. CD138 IHC (julia hough) 14. Re: eProtocols (Bill) 15. Re: Just three months????? (Michael LaFriniere) ---------------------------------------------------------------------- Message: 1 Date: Thu, 8 Apr 2010 11:16:31 +1000 From: "Tony Henwood" Subject: RE: [Histonet] frozen lung sections To: "Molinari, Betsy" , Message-ID: Content-Type: text/plain; charset="us-ascii" Betsy, I would dissolve the OCT in Hanks solution (or similar cell culture medium - not saline). Place in dilute OCT (50/50 with distilled water), place on stirrer until the lung settles in the OCT 30-60min. Blot with absorbent paper, refreeze in fresh OCT. It should cut quite well. I have used this previously on some tissue that was received the same way. It seems that the OCT had "freeze-dried" prior to receipt in my lab. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, 8 April 2010 12:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen lung sections Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS, then 4% paraformaldehyde then into sucrose. Can anyone clarify the procedure or offer advice? I am unfamiliar with this technique since most of my frozen sectioning is heart tissue and it does not present the same problem as the lung. Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 2 Date: Thu, 8 Apr 2010 08:11:50 +0400 From: Anne van Binsbergen Subject: Re: [Histonet] BREAST IMPLANTS AND HARDWARE To: "Vickroy, Jim" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 your path dept is being 'used' as a tracking system for these items - the charge is perfectly justifiable - we do it all the time tell the plastics guys to set up their own tracking system for these samples - less hassle for the lab!!! everything removed from a patient comes to my lab - except forskins from infants and certain debridements we have many 88300 charges regarding the release to be signed - from a medico-legal point of view it would be best to get someone to sign and get a copy of SS number (or some sort of ID) just in case my 2 cents worth AbuDhabiAnnie On 7 April 2010 22:17, Vickroy, Jim wrote: > Our pathology group charges a "gross only" charge for hardware and implants > removed from surgery that go to the pathology department. Our current policy > states that normally these devices do not have to be sent to pathology > however when the material is requested by the patient to be returned to the > patient or the patient's clinician it must go to the pathology department > for accessioning and documentation. Since we do a gross description and > work with the proper paperwork for release of the items we currently charge > an 88300 charge which is a gross only charge. Our plastics department > questions the necessity of this position and why we are charging for it. > Can anybody else share how they are handling these types of materials > removed at surgery? In addition our risk management team tells us that > these devices have to have a release signed by the patient before they can > be given to the patient or the patient's surgeon. > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 3 Date: Thu, 8 Apr 2010 07:02:45 -0400 From: "Marshall, Kimberly" Subject: [Histonet] Traveling Histo Tech To: "histonet@lists.utsouthwestern.edu" Message-ID: <136EE5CD3735EE4BB86FE88F4B6D0172D30C3D08@LKMVXCHMB22.ADVENTISTCORP.NET> Content-Type: text/plain; charset=us-ascii Hello all, A few years back I went on vacation and we used a traveling Histo tech to cover. Back then I thought it was a neat idea and said to myself, when the kids are grown and out of the house I am gonna do that. Well that time has come, so before I started making calls and researching it I wanted to ask my fellow Histo folks what they know about it. Is there still a market for traveling techs? Any information about this would sure be appreciated. Thanks in advance Kimberly Marshall HT(ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== ------------------------------ Message: 4 Date: Thu, 08 Apr 2010 06:10:46 -0500 From: "Nita Searcy" Subject: RE: [Histonet] TX Society meeting? To: "Stephen Feher" , , "Cheryl" Message-ID: <4BBD7365.5D38.00EF.0@swmail.sw.org> Content-Type: text/plain; charset="us-ascii" Website has all of the information Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Feher, Stephen" 4/7/2010 4:13 PM >>> Contact KDWYER3322@AOL.COM. April 23-25, Houston, TX. Be sure to sign up for the "Designing a LEAN Pathology Lab from Scratch" workshop on Saturday, April 24. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, April 06, 2010 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TX Society meeting? Any word on Texas' regional histology meeting? I'm looking for dates and the registration info for my team-- Thanks! Cheryl Kerry, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ------------------------------ Message: 5 Date: Thu, 8 Apr 2010 09:05:38 -0400 From: John PJ Coleman Subject: [Histonet] trich/bouin's/FS/Inst cleaning/mast cells To: histonet@lists.utsouthwestern.edu Message-ID: <90AB433A-AD51-4D0D-9CCC-DE8EE9E50B34@aol.com> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes Trichrome: Our Masson Trichrome procedure uses a combined phosphotungstic/phosphomolybdic acid step for 20 minutes @ RT without a rinse after, our smooth muscle stays RED.. Bouin's: Working in a rat research lab for years, we fixed all tissue in bouins for 24 hours, rinsed in water for about an hour and stored in 70% alcohol till processing. Is all your tissue post fixed in bouins or just selected items? Why not Bouin's as a primary fixative? We got great results on PTAH, Trichrome, Luxol Fast Blue stains. Frozens: CAP says monitor FS times, if 90% are not within 20 minutes, document the evaluation of why. ANP.11820 Instrument cleaning: We use TBQ which is virucidal and does not rust instruments like bleach does. Mast cells: We have not had unintentional mast cell staining on our fleet of Ultras using the either Ultraview or the alk phos kits. John PJ Coleman, HT(ASCP)QIHC ------------------------------ Message: 6 Date: Thu, 8 Apr 2010 09:54:02 -0400 From: "Weems, Joyce" Subject: [Histonet] eProtocols To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015D4256F6@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Hello Everyone, Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS? Thanks much!, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 7 Date: Thu, 8 Apr 2010 07:59:13 -0700 (PDT) From: Bernard Martin Subject: [Histonet] Sestrin 2 To: histonet@lists.utsouthwestern.edu Message-ID: <226491.17228.qm@web37901.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi all, I asked this a couple weeks ago, but thought I'd give it one more try- has anyone had any experience with Sestrin 2 for IHC? I can't find anything in the literature, and thought maybe some has tried it. There are ones available, but they all use the same picture, and I am concerned perhaps it doesn't work well. Funds are limited so we would like to get one that someone has had some success with. Thanks for any info you could provide! Bern ------------------------------ Message: 8 Date: Thu, 8 Apr 2010 11:05:47 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] eProtocols To: "Weems, Joyce" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I have printed off the CAP worksheets off the web site. We use them as work sheets for the pathologist. He checks off and fills in what is pertinent to the case, then the transcriptionist transcribes the Synoptic Report into the final report directly under the Final Diagnosis. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/08/2010 09:54 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] eProtocols Hello Everyone, Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS? Thanks much!, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 9 Date: Thu, 8 Apr 2010 10:17:09 -0500 From: Cheri Miller Subject: [Histonet] RE: Traveling Histo Tech To: "Marshall, Kimberly" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Only to say I am envious, good luck and have fun! Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 10 Date: Thu, 8 Apr 2010 16:33:01 +0100 From: "Edwards, Richard E." Subject: [Histonet] RE: Travelling Histo Tech To: 'Cheri Miller' , "Marshall, Kimberly" , "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8CFFB6D32@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" So you could sum it up thus:- "Have knife, will slice for a price". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: 08 April 2010 16:17 To: Marshall, Kimberly; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Traveling Histo Tech Only to say I am envious, good luck and have fun! Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 8 Apr 2010 16:38:03 +0100 From: "Thotakura, Anil Kumar" Subject: [Histonet] To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I have a problem with immunohistochmesitry. I am have raised my own antibody and it is not commercially available. This monoclonal antibody works fine for western blot. When it come to immunohistochemistry on mice liver frozen section, it is binding every where. I can see staining in my control as well. Any suggestion or guess's from you guys? Anil ------------------------------ Message: 12 Date: Thu, 8 Apr 2010 17:38:40 +0200 From: "Gudrun Lang" Subject: [Histonet] CD 25 protocol on Benchmark To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Benchmark-users! Can someone of you provide me with a protocol for CD 25 for FFPE tissue. We have purchased the antibody from Cell marque and have found nuclear staining in trephine bone marrow biopsy with mastocytosis. This specimen was decalcified with formic acid. Used protocol: CC1 mild, 1:10 for 40 min I would be happy about any help. Gudrun Lang ------------------------------ Message: 13 Date: Thu, 8 Apr 2010 17:27:26 +0100 From: julia hough Subject: [Histonet] CD138 IHC To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear all. Does anyone have a protocol for the immunohistochemistry staining of CD138 on paraffin embedded mouse tibia, including the fixation and decalcification protocol? Thanks BW Julia _________________________________________________________________ http://clk.atdmt.com/UKM/go/197222280/direct/01/ Do you have a story that started on Hotmail? Tell us now ------------------------------ Message: 14 Date: Thu, 8 Apr 2010 11:30:58 -0500 From: Bill Subject: Re: [Histonet] eProtocols To: "Weems, Joyce" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" At 9:54 AM -0400 4/8/10, Weems, Joyce wrote: >Are any of your pathologists using the Cancer eProtocols provided by CAP? If so you would you let me know of your experiences and how you interfaced it with your LIS?< We export data from our LIS and pick it up in Word merge templates. We then use autotext to add the appropriate protocol info (modified) for the diagnosis. Bill ------------------------------ Message: 15 Date: Thu, 08 Apr 2010 12:47:14 -0400 From: "Michael LaFriniere" Subject: Re: [Histonet] Just three months????? To: , "Joe Nocito" , "Jeffrey Silverman" Message-ID: <4BBDD046.588C.00AF.0@csmlab.com> Content-Type: text/plain; charset=US-ASCII FYI The Grandfather clause still stands as it always had.... Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 4/1/2010 at 8:46:10 PM, "Joe Nocito" wrote: I see that now, wasn't the first time I was wrong today, probably wouldn't be the last either ----- Original Message ----- From: "Jeffrey Silverman" To: Sent: Wednesday, March 31, 2010 5:03 PM Subject: [Histonet] Just three months????? CAP is now saying no more gross processing of small things that are entirely submitted- it's all gross examination now whether we mean straining currettings into a cassette or dissecting a complex cancer resection. Dumb as all get out if you ask me. As for the three month training thing, Joe, I'm not so sure about that. They seem to spell out specific amounts of college education required IN ADDITION to training in the laboratory. The requirement first spells out two education choices- 1: an earned associates degree in medical laboratory science OR 2:Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education MUST (caps mine) include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. So in addition to all the college courses, which we all know you need to count and measure six endoscopic biopsies and put them in a cassette, much more training and experience needed than what an unregistered on the job tech needs to orient and embed them properly LOL!!! CLIA then requires that the individual must have (additional) laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. Now there are grandfathering clauses in CLIA which may enable folks (like myself) to continue to gross. I haven't digested that yet, but I'm ready to get that job in Pathmark if necessary. Sheesh, does it ever end? Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 10 **************************************** From k84as <@t> yahoo.com Thu Apr 8 15:11:40 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Thu Apr 8 15:11:45 2010 Subject: [Histonet] fixtives Message-ID: <221823.6473.qm@web112613.mail.gq1.yahoo.com> are you using bouin's , susa or formol zinker?? how many times do you use the same fixtive? i know that zinker need to be freshly prepaired ? thanxs ? ? mohamed From eridana <@t> cox.net Thu Apr 8 16:04:26 2010 From: eridana <@t> cox.net (Eridana) Date: Thu Apr 8 16:04:30 2010 Subject: [Histonet] Tungesten Carbide Knives and araldite Message-ID: <20100408170426.HO0JL.7155.imail@fed1rmwml31> I have a tiny project using araldite on mouse nerves and wondered if anyone could recommend the tungsten carbide knife that Ted Pella cat #121-50 or any other company sells. I cut methacrylate ages ago with glass knives but wonder if the tungesten carbide blade might be a easier and way less expensive option than a $9k Leica glass knife breaker. I used the Sorvall breaker but it looks to be discontinued. If anyone has an opinion on araldite, I would appreciate that too. Thanks Donna Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2010 San Diego, CA 92093 858 534 7438 From jshea121 <@t> roadrunner.com Thu Apr 8 16:18:49 2010 From: jshea121 <@t> roadrunner.com (Shea's) Date: Thu Apr 8 16:18:57 2010 Subject: [Histonet] eProtocols Message-ID: Our Pathologist found it more time consuming to delete lines that were non-applicable than to dictate what they do want from the CAP Cancer Protocols: 1. I copied and pasted the commonly used protocols into the canned text dictionary (in meditech, magic) then each pathologist will edit it to meet their usual needs, and save it again (giving it a name they will remember). To use it, they go into the Diagnosis data section, type the canned text, then they still add/delete lines that are applicable, but it is still a bit more user friendly. They each have a thick binder notebook of the protocols in print to follow along if needed. 2. Now that we use dragon voice recognition, they have all made up "macros" for the most commonly used ones. They will go to the diagnosis data section and voice the command for the protocol. A macro/canned text version will appear. They will also have a printed copy from CAP Protocol to follow along if they need to add or delete anything. Either way they find it easier than deleting lines. Also I should add, this is easiest for them since we do not have a pathology secretary or transcriptionist. Jan From raestask <@t> grics.net Thu Apr 8 16:57:09 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Thu Apr 8 16:57:19 2010 Subject: [Histonet] Illinois Annual Meeting Message-ID: <9926848D44B84D75A452E9F844BEA37D@your4105e587b6> This year the Illinois Society for Histotechnologists is holding their Annual Spring meeting at Eaglewood Resort in Itaska, Illinois. The dates are May 13-14, 2010. Unfortunately it seems as though we are trying to follow the example of the Republican National Committee and have an incorrect phone number for room reservations at the resort. The correct number is 1-877-285-6150. Be sure to mention that you are with the ISH when making your reservation in order to receive the convention rates. Reservations must be made by April 12 in order to receive the reduced rates. Check out the Eaglewood Resort at www.eaglewoodresort.com to see all of the amenities waiting for you! If you would like an electronic brochure, contact myself at raestask@grics.net. Rae Ann Staskiewicz From kris <@t> idxpathology.com Thu Apr 8 16:57:57 2010 From: kris <@t> idxpathology.com (Scherer, Kris) Date: Thu Apr 8 16:58:35 2010 Subject: [Histonet] Histo Aid / Histotech In-Reply-To: <0C15E5FC826B334BA019E489846D023578D3D2E42E@RTWECS03.lca.labcorp.com> References: <0C15E5FC826B334BA019E489846D023578D3D2E42E@RTWECS03.lca.labcorp.com> Message-ID: <0C15E5FC826B334BA019E489846D023578D3D2E42F@RTWECS03.lca.labcorp.com> We would like to hire a histology aid. What are the educational requirements for a histology aid, compared to a histotechnician? --Kris ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA. From cscampbe <@t> uci.edu Thu Apr 8 17:41:34 2010 From: cscampbe <@t> uci.edu (cscampbe@uci.edu) Date: Thu Apr 8 17:41:38 2010 Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? Message-ID: <6cf6a7d7a3c01ccd395014777997c191.squirrel@webmail.uci.edu> Hi Histonet, I am currently using the protocol from Stainsfile for staining heart tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm My lab had Fast Green readily available so I substituted it for Light Green in Solution C. The slides have not been coming out well. I failed to account for the difference in timing between FG and LG - running the slide in FG for the full 10 minutes. Has anyone used FG in Masson's Trichrome? If so, how long did it take to get the desired colors in the solution? Or, is Fast Green a poor substitute for Light Green? Thanks for your advice! -Colin From Jan.Minshew <@t> leica-microsystems.com Thu Apr 8 17:50:19 2010 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Thu Apr 8 17:52:22 2010 Subject: [Histonet] New job opportunities Message-ID: Hello everyone, Leica Microsystems is adding new positions and would like to hear from those of you who might be interested. The positions are for Field Support Specialists (FSS) and will be based in Arizona, Washington state, New England, Florida and Montreal. Applicants for the Montreal position must be fluent in both French and English. Some of the responsibilities include in-field support for IHC, conducting demonstrations and post-sales customer training, performing validations and optimizations and providing technical support for remote problem solving. If you are interested in trying something new and different and feel that you are qualified, please contact Linda Jordan, HR Recruiter at hrcoord@leica-microsystems.com. Kind regards, Jan Minshew Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Office: 847.405.7051 Cell: 847.970.8468 Fax: 847.405.6560 www.leica-microsystems.com Click Here for this month's special offers! http://www.leica-microsystems.com/bsdspecial ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From histotech <@t> imagesbyhopper.com Thu Apr 8 20:32:17 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Apr 8 20:32:26 2010 Subject: [Histonet] CPT coding In-Reply-To: Message-ID: <024B13D65167446F9F18F566E4CEFF0B@hopperPC> Hi Histonetters! We have run into a question about CPT charges and I was hoping to get a more definitive answer than what we currently have. I know if you have two tonsils in one container, one with a stitch and one without, you can charge for both tonsils. How about if you have one container with a placenta and a stillborn fetus? One suggestion is to charge an 88307 and the other is to charge 88300 AND an 88307 (to cover the gross only on the fetus and the tissue that was submitted for the placenta). What is the standard, shall I say legal?, way of charging for these specimens? Must you choose one of the charges or are we allowed to use both charges? Thanks for any light you can shed on this! Michelle From Susan.Walzer <@t> HCAHealthcare.com Fri Apr 9 02:10:44 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Apr 9 02:10:52 2010 Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? In-Reply-To: <6cf6a7d7a3c01ccd395014777997c191.squirrel@webmail.uci.edu> References: <6cf6a7d7a3c01ccd395014777997c191.squirrel@webmail.uci.edu> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AF489C24F@FWDCWPMSGCMS09.hca.corpad.net> We use 5 minutes on FG. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cscampbe@uci.edu Sent: Thursday, April 08, 2010 6:42 PM To: HistoNet Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? Hi Histonet, I am currently using the protocol from Stainsfile for staining heart tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm My lab had Fast Green readily available so I substituted it for Light Green in Solution C. The slides have not been coming out well. I failed to account for the difference in timing between FG and LG - running the slide in FG for the full 10 minutes. Has anyone used FG in Masson's Trichrome? If so, how long did it take to get the desired colors in the solution? Or, is Fast Green a poor substitute for Light Green? Thanks for your advice! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bruski33 <@t> aol.com Fri Apr 9 08:11:59 2010 From: bruski33 <@t> aol.com (bruski33@aol.com) Date: Fri Apr 9 08:12:16 2010 Subject: [Histonet] Immuno staining on plastic sections Message-ID: <8CCA5FD5F0294BF-1D1C-FA34@webmail-d015.sysops.aol.com> We are trying to do immuno staining on plastic sections and are not having much luck. Does anyone have any experience with this? We are embedding in technovit 8100. It is a device with encapsulated VEGFR cells. I have been told that we might have to do an etching step. Any help would be greatly appreciated. Bruce From JoelI <@t> mcclainlab.com Fri Apr 9 08:40:00 2010 From: JoelI <@t> mcclainlab.com (Joel Israel) Date: Fri Apr 9 08:34:13 2010 Subject: [Histonet] Cd31 in pigs. Message-ID: Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel From Wanda.Smith <@t> HCAhealthcare.com Fri Apr 9 08:39:44 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Apr 9 08:40:15 2010 Subject: [Histonet] Does a Cytotechnologist Meet CAP Grossing Requirements? Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1388AE0A24@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All and Happy Friday!!!!! In your opinion, does a graduate from a Cytotechnologist Program (ASCP) with additional documented training meet the new requirement for grossing as high complexity testing personnel under CLIA??? Our PA that works for our Pathologist group is a cytotech who did additional training in grossing. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From Janice.Mahoney <@t> alegent.org Fri Apr 9 09:05:24 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Apr 9 09:05:50 2010 Subject: [Histonet] CPT coding In-Reply-To: <024B13D65167446F9F18F566E4CEFF0B@hopperPC> References: <024B13D65167446F9F18F566E4CEFF0B@hopperPC> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A094@EXCHMBC2.ad.ah.local> They are two distinct specimens, if you diagnose the two separately you may bill for two separate specimens even though they were in one container. Jan M Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Wanda.Smith <@t> HCAhealthcare.com Fri Apr 9 09:12:41 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Apr 9 09:13:15 2010 Subject: [Histonet] CPT coding In-Reply-To: <024B13D65167446F9F18F566E4CEFF0B@hopperPC> References: <024B13D65167446F9F18F566E4CEFF0B@hopperPC> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1388AE0AFF@NADCWPMSGCMS03.hca.corpad.net> We do a surgical examination on the placenta and the fetus when they are in the same container. We charge 88307 for the placenta and 88309 for the fetus. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, April 08, 2010 9:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding Hi Histonetters! We have run into a question about CPT charges and I was hoping to get a more definitive answer than what we currently have. I know if you have two tonsils in one container, one with a stitch and one without, you can charge for both tonsils. How about if you have one container with a placenta and a stillborn fetus? One suggestion is to charge an 88307 and the other is to charge 88300 AND an 88307 (to cover the gross only on the fetus and the tissue that was submitted for the placenta). What is the standard, shall I say legal?, way of charging for these specimens? Must you choose one of the charges or are we allowed to use both charges? Thanks for any light you can shed on this! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Apr 9 09:18:50 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Apr 9 09:18:58 2010 Subject: [Histonet] RE: Does a Cytotechnologist Meet CAP Grossing Requirements? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1388AE0A24@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1388AE0A24@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A095@EXCHMBC2.ad.ah.local> Wanda, If they do not have a BS as well as their CT(ASCP) they need to have documentation of the required number of hours of biology and chemistry in addition to the training. If so, you should be ok. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Friday, April 09, 2010 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does a Cytotechnologist Meet CAP Grossing Requirements? Good Morning to All and Happy Friday!!!!! In your opinion, does a graduate from a Cytotechnologist Program (ASCP) with additional documented training meet the new requirement for grossing as high complexity testing personnel under CLIA??? Our PA that works for our Pathologist group is a cytotech who did additional training in grossing. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From cmiller <@t> physlab.com Fri Apr 9 10:31:32 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Apr 9 10:31:41 2010 Subject: [Histonet] charge for pin 4 cocktail Message-ID: What are people charging Pin 4 p63,CK5-14and P504S......88342 x 3 or 4 Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From marktarango <@t> gmail.com Fri Apr 9 10:36:22 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 9 10:36:27 2010 Subject: [Histonet] charge for pin 4 cocktail In-Reply-To: References: Message-ID: x3 only because you can't tell the difference between these basal cell markers CK5 and CK14 (they stain the same cells and both in the cytoplasm). Mark Tarango On Fri, Apr 9, 2010 at 8:31 AM, Cheri Miller wrote: > What are people charging Pin 4 p63,CK5-14and P504S......88342 x 3 or 4 > > Cheryl Miller HT ASCP cm > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Fri Apr 9 10:39:33 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Apr 9 10:39:39 2010 Subject: [Histonet] RE: charge for pin 4 cocktail In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015D425942@CHEXCMS10.one.ads.che.org> X 3 because two of the markers cannot be distinguished. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, April 09, 2010 11:32 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] charge for pin 4 cocktail What are people charging Pin 4 p63,CK5-14and P504S......88342 x 3 or 4 Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Bill.Tench <@t> pph.org Fri Apr 9 10:57:19 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Apr 9 10:57:28 2010 Subject: [Histonet] CPT coding question Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02862F62@MAIL1.pph.local> Michelle, the example that you raised of having two tonsils, one with a stitch, isn't quite the same as your problem. The two tonsils would otherwise not be identifiable as left or right. That is not a problem with a fetus and a placenta. Each is separately identifiable and each should be coded separately. You will simplify any coding review if each is a separate line on your report. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From jm.lapointe <@t> accellab.com Fri Apr 9 10:59:44 2010 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Fri Apr 9 11:00:35 2010 Subject: [Histonet] Cd31 in pigs In-Reply-To: <201004091534.o39FYKC0021829@gateway5.lastspam.com> References: <201004091534.o39FYKC0021829@gateway5.lastspam.com> Message-ID: Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Qu?bec, Canada J7H 1N8 jm.lapointe@accellab.com ? ? Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel From jkiernan <@t> uwo.ca Fri Apr 9 11:08:48 2010 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Apr 9 11:08:55 2010 Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? Message-ID: "The slides have not been coming out well." is not very informative! Your problems may have nothing to do with which dye you are using as the collagen stain. There are plenty of other things that can make a multi-step trichrome method give unexpected results. Fast green FCF (C.I. 42053) is usually considered superior to light green SF (C.I. 42095) on account of being less prone to fading. Be sure you have the correct dye, and that it is from a batch certified by the Biological Stain Commission (B.S.C. - see the label on the bottle of dye powder, which will also tell you the dye content). If you buy a ready-made solution, check with the supplier. The minimum acceptable dye content for certification of fast green FCF (85%) is higher than the minimum for light green SF (65%). A 2% solution of the former might therefore have greater tinctorial power than a 2% solution of the latter. The B.S.C. tests both dyes as substitutes for aniline blue in Gomori's one-step trichrome method; the concentration of blue or green dye in the mixture is 0.3%, irrespective of the dye content of the powder. Gomori's is a more stringent test for dyes than Masson's trichrome method because there is no visual/manual control of the staining. A dye that works with Gomori's method should work well with Masson's. The Masson variant that you have been trying (from Bryan Llewellyn's excellent web site, http://stainsfile.info/StainsFile/stain/conektv/masson.htm) is well documented. Follow up some references from Bryan's citation of the Bancroft & Stevens book, and learn the reasons for all the 11 steps of the modified Masson technique. The current edition (6th, 2008) of "Theory and Practice" is edited by Bancroft & Gamble: ISBN 9780443102790. My textbook (ISBN 97819048422) covers trichrome methods in Chapter 8, with plenty of references to scientific/scholarly literature, much of which is now available on the internet, especially if you can go through a university or public library's web interface. There is a well ilustrated chapter on troubleshooting trichromes, by Vinnie Della Speranza, in R.W.Brown's (2009) "Common Problems" book, ISBN 9780930304959 (pp. 95-101). A rinse in tap water at any stage after after washing out the iron-haematoxylin nuclear stain can weaken either the red or the blue/green component of any trichrome method. So can "graded alcohols" for the final dehydration of well stained slides. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: cscampbe@uci.edu Date: Thursday, April 8, 2010 18:42 Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? To: HistoNet > Hi Histonet, > > I am currently using the protocol from Stainsfile for staining heart > tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm > > My lab had Fast Green readily available so I substituted it for Light > Green in Solution C. The slides have not been coming out well. I > failed to > account for the difference in timing between FG and LG - running > the slide > in FG for the full 10 minutes. Has anyone used FG in Masson's > Trichrome?If so, how long did it take to get the desired colors > in the solution? Or, > is Fast Green a poor substitute for Light Green? > > Thanks for your advice! > -Colin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Fri Apr 9 11:13:56 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Apr 9 11:14:02 2010 Subject: [Histonet] Immuno staining on plastic sections In-Reply-To: <8CCA5FD5F0294BF-1D1C-FA34@webmail-d015.sysops.aol.com> References: <8CCA5FD5F0294BF-1D1C-FA34@webmail-d015.sysops.aol.com> Message-ID: A few years ago I took a workshop at the NSH presented by Neil Hand. He basically provided an account of his experience in working with something like over 100 antibodies used on resin (MMA) embedded bone and possibly other tissues. Maybe you can look his information up on the NSH website and contact him directly? With regards to an "etching step", there are several posts that describe everything from HCl to formic acid to sodium ethoxide. In fact, I just found this posting from Gayle Callis back in 2004. Hope this helps! Jack [Histonet] Repy on immunostaining with Technovit 8100 / glycol methacrylate Gayle Callis gcallis <@t> montana.edu Thu Aug 12 10:13:55 CDT 2004 GMA is not a very ideal embedding media for immunohistochemistry, you would be far better off with paraffin using this antibody. GMA cannot be removed once polymerized nor sodium ethoxide (generally reserved for electron microscopy resins) etched with much success, you would be better off embedding in methylmethacrylate and remove the plastic entirely. This has been done with great success by Neil Hand (he has publications) using warm xylene, but he used stringent pressure cooker retrieval and worked with human tissue. The problem with GMA is it prevents the immunoglobulins from reaching antigenic sites, as the GMA is hydrophobic plus it can't be removed once polymerized. It will soften in the presence of water. Also, as GMA polymerizes it becomes very hot due to exothermic reaction unless you control this temperature by letting your blocks polymerize on ice in a refrigerator?? GMA with IHC problems have been discussed at length on Histonet many times, go to Histonet archives and search at www.histosearch.org. Personally, I don't think the product "suggestion" is correct, as it only "suggests" but does not say it WILL work. That is probably the reason you don't find it in the literature! Many people have experienced failure of IHC on GMA embedded tissues. I think some have had success with immunofluroescence using immunoglobulins and not a lot of antibodies either. It was a tedious stringent protocol described in a symposium talk. I think you will find in Histonet commentary, that most people attempting IHC/GMA suggest going to another embedding media. Also, CD68 ED1 is a Mouse antiRat, rather than a rat antiMouse (clone FA-11). ED1 and other rat macrophage markers, ED2, ED3, have been discussed on Histonet as FFPE tissues including retrieval for IHC. The staining was very straightforward as was retrieval described and solvents used plus heat of paraffin processing did not damage antigen. ED1, when used in on rat tissue, works well on paraffin sections, although we prefer frozen sections to avoid all aldehyde fixation and no retrieval. When we do frozen sections, the ED1 is diluted out 1:3000 or so, it will not be so dilute for paraffin sections and we detected with secondary to mouse IgG1 isotype (Southern Biotechnology) SA-HRP, and AEC chromogen. Staining pattern is spectacular in a rat spleen, normal positive control. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 > To: histonet@lists.utsouthwestern.edu > Date: Fri, 9 Apr 2010 09:11:59 -0400 > From: bruski33@aol.com > Subject: [Histonet] Immuno staining on plastic sections > > > > We are trying to do immuno staining on plastic sections and are not having much luck. Does anyone have any experience with this? We are embedding in technovit 8100. It is a device with encapsulated VEGFR cells. I have been told that we might have to do an etching step. Any help would be greatly appreciated. > Bruce > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Fri Apr 9 11:16:04 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Apr 9 11:16:30 2010 Subject: [Histonet] IHC on FNA Message-ID: <000001cad7ff$f44b0530$dce10f90$@com> Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From shive003 <@t> umn.edu Fri Apr 9 11:24:09 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Apr 9 11:24:13 2010 Subject: [Histonet] Cd31 in pigs References: <201004091534.o39FYKC0021829@gateway5.lastspam.com> Message-ID: <6BCCE0E25DD04549A2E2145F93AB4133@auxs.umn.edu> Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Jean-Martin Lapointe" To: Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Qu?bec, Canada J7H 1N8 jm.lapointe@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BenatM <@t> gosh.nhs.uk Fri Apr 9 11:26:44 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Fri Apr 9 11:32:26 2010 Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? In-Reply-To: References: Message-ID: <4BBF635F.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Colin, If you havin difficulty with MT try the Gomori One Step 1. Sections to water. 2. Pretreat in 3% Potassium dichromate for 1hr at 60?C 3. Stain nuclei with celestin blue/Mayer's haematoxylin. 5 min 4. Wash in tap water. 5. Differentiate and blue. 6. Stain with chromotrope green mixture --- 10 mins. 7. Rinse in distilled water 8. Blot and dehydrate from absolute alcohol ,clear and mount. Results Muscle and fibrin - Red RBC's - Red Collagen - Green MASSON TRICHROME (ONE-STEP) Ingredients: Chromotrope 2R 0.6 g. Fast Green FCF 0.3 g. Phosphotungstic acid 0.6 g. Glacial acetic acid 1 ml. Distilled water 100 ml. METHOD:- Dissolve the chromotrope 2R, the fast green and the phosphotungstic acid in the water and add the glacial acetic acid. Hope this help Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk >>> John Kiernan 09/04/2010 17:08 >>> "The slides have not been coming out well." is not very informative! Your problems may have nothing to do with which dye you are using as the collagen stain. There are plenty of other things that can make a multi-step trichrome method give unexpected results. Fast green FCF (C.I. 42053) is usually considered superior to light green SF (C.I. 42095) on account of being less prone to fading. Be sure you have the correct dye, and that it is from a batch certified by the Biological Stain Commission (B.S.C. - see the label on the bottle of dye powder, which will also tell you the dye content). If you buy a ready-made solution, check with the supplier. The minimum acceptable dye content for certification of fast green FCF (85%) is higher than the minimum for light green SF (65%). A 2% solution of the former might therefore have greater tinctorial power than a 2% solution of the latter. The B.S.C. tests both dyes as substitutes for aniline blue in Gomori's one-step trichrome method; the concentration of blue or green dye in the mixture is 0.3%, irrespective of the dye content of the powder. Gomori's is a more stringent test for dyes than Masson's trichrome method because there is no visual/manual control of the staining. A dye that works with Gomori's method should work well with Masson's. The Masson variant that you have been trying (from Bryan Llewellyn's excellent web site, http://stainsfile.info/StainsFile/stain/conektv/masson.htm) is well documented. Follow up some references from Bryan's citation of the Bancroft & Stevens book, and learn the reasons for all the 11 steps of the modified Masson technique. The current edition (6th, 2008) of "Theory and Practice" is edited by Bancroft & Gamble: ISBN 9780443102790. My textbook (ISBN 97819048422) covers trichrome methods in Chapter 8, with plenty of references to scientific/scholarly literature, much of which is now available on the internet, especially if you can go through a university or public library's web interface. There is a well ilustrated chapter on troubleshooting trichromes, by Vinnie Della Speranza, in R.W.Brown's (2009) "Common Problems" book, ISBN 9780930304959 (pp. 95-101). A rinse in tap water at any stage after after washing out the iron-haematoxylin nuclear stain can weaken either the red or the blue/green component of any trichrome method. So can "graded alcohols" for the final dehydration of well stained slides. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: cscampbe@uci.edu Date: Thursday, April 8, 2010 18:42 Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green? To: HistoNet > Hi Histonet, > > I am currently using the protocol from Stainsfile for staining heart > tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm > > My lab had Fast Green readily available so I substituted it for Light > Green in Solution C. The slides have not been coming out well. I > failed to > account for the difference in timing between FG and LG - running > the slide > in FG for the full 10 minutes. Has anyone used FG in Masson's > Trichrome?If so, how long did it take to get the desired colors > in the solution? Or, > is Fast Green a poor substitute for Light Green? > > Thanks for your advice! > -Colin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From k84as <@t> yahoo.com Fri Apr 9 11:38:14 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Apr 9 11:38:17 2010 Subject: [Histonet] enzymes significance? Message-ID: <329829.43027.qm@web112611.mail.gq1.yahoo.com> dear histonetters as i'm learning HC & IHC?i need to know a reference or internet site that could help me to know the significance of different enzymes in tissues?? any help in these!? a second quistion please i'm trying to locat Alk. Phosphatase in chicken intestine and i found in old reference the steps and reagent used for demonstration of it. i need to know is there a commercially avaliable kits ??? please share me your protocols ? any help will be appreciated thanx mohamed From LSebree <@t> uwhealth.org Fri Apr 9 11:40:13 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Apr 9 11:40:21 2010 Subject: [Histonet] IHC on FNA In-Reply-To: <000001cad7ff$f44b0530$dce10f90$@com> References: <000001cad7ff$f44b0530$dce10f90$@com> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E05@UWHC-MAIL01.uwhis.hosp.wisc.edu> This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Apr 9 12:07:12 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 9 12:07:15 2010 Subject: [Histonet] IHC on FNA In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399E05@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DB9@is-e2k3.grhs.net> Great points, Linda! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, April 09, 2010 11:40 AM To: Cynthia Pyse; Histonet Subject: RE: [Histonet] IHC on FNA This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Fri Apr 9 12:15:23 2010 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Apr 9 12:15:27 2010 Subject: [Histonet] PT or as need work in WI/ILL Message-ID: <538636.72379.qm@web38205.mail.mud.yahoo.com> Good afternoon all, Just my periodic post looking for work in the So.WI/No.Ill area.? Steve From cpyse <@t> x-celllab.com Fri Apr 9 12:46:53 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Apr 9 12:47:19 2010 Subject: [Histonet] IHC on FNA In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738399E05@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <000001cad7ff$f44b0530$dce10f90$@com> <8C023B4AB999614BA4791BAEB26E2738399E05@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <000b01cad80c$a4537320$ecfa5960$@com> Linda I agree with you that is why I asked the question. We do not have a cryostat, so frozen sections are out of the question. I thought of making cytospin slides out of positive fluid but then you run into the problem of any positive cells making it onto the slide. Would any of you run the FNA slide without pretreatment and the FFPE control with the pretreatment? I appreciate everyone suggestions. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: Sebree Linda A [mailto:LSebree@uwhealth.org] Sent: Friday, April 09, 2010 12:40 PM To: Cynthia Pyse; Histonet Subject: RE: [Histonet] IHC on FNA This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Apr 9 12:48:35 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Apr 9 12:48:39 2010 Subject: [Histonet] Job in Little Rock Message-ID: <100483227.14311270835314979.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Hi All, UAMS (University of Arkansas for Medical Sciences) is looking for a full time histologist.? Canidate must be a regesterd HT or HTL or eligible for ASCP registry?for?our Histology Laboratory.? It is on the UAMS website under Special Procedures Tech in the Technical area.? If you are interested please fill out an application or contact me directly.? Recuiters please note we are not allowed to use outside services.? Pamela Marcum Anatomic Pathology Manager UAMS Little Rock AR 501-686-5941 From lwilson <@t> bethyl.com Fri Apr 9 14:43:35 2010 From: lwilson <@t> bethyl.com (Liz Wilson) Date: Fri Apr 9 14:44:52 2010 Subject: [Histonet] Re: Cd31 in pigs In-Reply-To: <20100409170527.D8F1C2720045@mx01.mfg.onr.siteprotect.com> References: <20100409170527.D8F1C2720045@mx01.mfg.onr.siteprotect.com> Message-ID: Serotec's mouse anti-porcine CD31 (MCA1746) will work well in fresh, frozen, acetone-fixed pig tissues. It is very good for angiogenesis. It does not work with FFPE tissues. Liz Wilson Bethyl Labs ------------------------------ Message: 4 Date: Fri, 9 Apr 2010 11:59:44 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] Cd31 in pigs To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Quibec, Canada J7H 1N8 jm.lapointe@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel From pathmaster <@t> yahoo.com Fri Apr 9 17:00:55 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Fri Apr 9 17:00:58 2010 Subject: [Histonet] IHC controls for FNA's Message-ID: <270657.68589.qm@web111108.mail.gq1.yahoo.com> It would depend on what type of tissue/ cells you are staining and what antibodies.? Why not obtain some of the fresh tissue from your most commonly performed (and IHC stained) type of cases, or from tissues that you know would be positive for the antibodies in question and do an FNA on it yourself. Aspirate some cells and make smears and fix them as you do your FNA's. It may be a problem to do this contemporaneously but maybe storing some smears in 95% alcohol would be appropriate. Jeff Silverman From pruegg <@t> ihctech.net Sat Apr 10 11:33:02 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 10 11:33:47 2010 Subject: SPAM-LOW: [Histonet] Re: Cd31 in pigs In-Reply-To: References: <20100409170527.D8F1C2720045@mx01.mfg.onr.siteprotect.com> Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up the method at work on Monday and send it to you if you contact me again to remind me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Wilson Sent: Friday, April 09, 2010 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Cd31 in pigs Serotec's mouse anti-porcine CD31 (MCA1746) will work well in fresh, frozen, acetone-fixed pig tissues. It is very good for angiogenesis. It does not work with FFPE tissues. Liz Wilson Bethyl Labs ------------------------------ Message: 4 Date: Fri, 9 Apr 2010 11:59:44 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] Cd31 in pigs To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Quibec, Canada J7H 1N8 jm.lapointe@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Apr 10 11:35:59 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 10 11:36:39 2010 Subject: SPAM-LOW: [Histonet] enzymes significance? In-Reply-To: <329829.43027.qm@web112611.mail.gq1.yahoo.com> References: <329829.43027.qm@web112611.mail.gq1.yahoo.com> Message-ID: <067492488C2F419A991C2E95E400DE2A@prueggihctechlt> I think Sigma still sells enzyme histochemical kits for Alk.Phos and TRAP, they are listed as Leukocyte acid phosphotase or Leukocyte alkaline phosphotase, contact me at work next week and I can give you the order info as I do still have these kits in my fridge. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, April 09, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] enzymes significance? dear histonetters as i'm learning HC & IHC?i need to know a reference or internet site that could help me to know the significance of different enzymes in tissues?? any help in these!? a second quistion please i'm trying to locat Alk. Phosphatase in chicken intestine and i found in old reference the steps and reagent used for demonstration of it. i need to know is there a commercially avaliable kits ??? please share me your protocols ? any help will be appreciated thanx mohamed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Apr 10 11:39:30 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 10 11:40:14 2010 Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs In-Reply-To: <6BCCE0E25DD04549A2E2145F93AB4133@auxs.umn.edu> References: <201004091534.o39FYKC0021829@gateway5.lastspam.com> <6BCCE0E25DD04549A2E2145F93AB4133@auxs.umn.edu> Message-ID: Yea you can use F8 and SMA for vessel lining cells but those will not pick up all the developing endothelial cells like cd31 or cd34. There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig, I will try it and see, it works great on mouse tissue ffpe. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Friday, April 09, 2010 10:24 AM To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Jean-Martin Lapointe" To: Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Qu?bec, Canada J7H 1N8 jm.lapointe@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Sat Apr 10 12:16:50 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Apr 10 12:16:54 2010 Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs In-Reply-To: Message-ID: Patsy How do you order that rat anti-mouse CD31. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, April 10, 2010 10:40 AM To: 'Jan Shivers'; 'Jean-Martin Lapointe'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs Yea you can use F8 and SMA for vessel lining cells but those will not pick up all the developing endothelial cells like cd31 or cd34. There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig, I will try it and see, it works great on mouse tissue ffpe. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Friday, April 09, 2010 10:24 AM To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Jean-Martin Lapointe" To: Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Qu?bec, Canada J7H 1N8 jm.lapointe@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suhyoung.jeong <@t> gmail.com Sat Apr 10 12:19:41 2010 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Sat Apr 10 12:19:45 2010 Subject: [Histonet] Mouse brain mitochondrial marker? Message-ID: Dear everyone, I'm trying to double-label mouse brain mitochondria with my rabbit antibody on paraffin section. I had tried anti-Tom20 (mouse, Santa Cruz) with biotin-streptavidin amplification, but the signal is very weak. So far, I had tried this antibody on cells and it was super-strong. I'm going to try for the TSA amplification, but wondering if anybody has experience and/or recommendation. Thank you in advance, Sincerely, Suh From k84as <@t> yahoo.com Sun Apr 11 16:41:47 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sun Apr 11 16:41:50 2010 Subject: [Histonet] why no response??!! Message-ID: <414281.6801.qm@web112613.mail.gq1.yahoo.com> dear all i have posted few days ago a quistion about enzyme significance in tissue?? eg. why i looking for alkaline phosphatase??? what it indicat?? ?i need a source or a sheet answering me ? and i have nearly no response from you any help her?? ? thanx mohamed From sbreeden <@t> nmda.nmsu.edu Mon Apr 12 07:06:02 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Apr 12 07:06:08 2010 Subject: [Histonet] HISTO STORIES Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4700C@nmdamailsvr.nmda.ad.nmsu.edu> I have sent out the "collection" of histo stories but need email from each one of the following people: greenjumpyone, Thom Jensen, Valerie Rodriguez, Sharon Campbell, Mary Abosso, Janice Mahoney, Valerie Hannen, Valantou Grover, Glen Dawson, Bernice Frederick, Sharon Beckham. When I forwarded emails posted to Histonet to my home email, all addresses showed "on behalf of..." and not your actual email address. Send them to me here at work today and you'll get immediate gratification (a copy of the Stories). If you did not request a copy but would like one, email me and here it'll come! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lwilson <@t> bethyl.com Mon Apr 12 09:07:53 2010 From: lwilson <@t> bethyl.com (Liz Wilson) Date: Mon Apr 12 09:09:15 2010 Subject: [Histonet] RE: CD31 in pigs In-Reply-To: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> References: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> Message-ID: Hi Patsy, I would love to try the method! Thanks -Liz Liz Wilson Bethyl Labs ____________________________________________________________________________ Message: 7 Date: Sat, 10 Apr 2010 10:33:02 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs To: "'Liz Wilson'" , Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up the method at work on Monday and send it to you if you contact me again to remind me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From JoelI <@t> mcclainlab.com Mon Apr 12 11:21:34 2010 From: JoelI <@t> mcclainlab.com (Joel Israel) Date: Mon Apr 12 11:16:52 2010 Subject: [Histonet] RE: CD31 in pigs In-Reply-To: References: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> Message-ID: Please count me in too! I would love a procedure that works and has been tested. Thanks- Joel -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Wilson Sent: Monday, April 12, 2010 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CD31 in pigs Hi Patsy, I would love to try the method! Thanks -Liz Liz Wilson Bethyl Labs ________________________________________________________________________ ____ Message: 7 Date: Sat, 10 Apr 2010 10:33:02 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs To: "'Liz Wilson'" , Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up the method at work on Monday and send it to you if you contact me again to remind me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lori.garcia <@t> medtronic.com Mon Apr 12 11:54:13 2010 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Mon Apr 12 11:54:39 2010 Subject: [Histonet] RE: CD31 in pigs In-Reply-To: References: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> Message-ID: <5A8A2A45BE610D459A95CD6A9102102AF820690C@STSM1BMSGM04.ent.core.medtronic.com> Ditto!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Israel Sent: Monday, April 12, 2010 9:22 AM To: Liz Wilson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: CD31 in pigs Please count me in too! I would love a procedure that works and has been tested. Thanks- Joel -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Wilson Sent: Monday, April 12, 2010 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CD31 in pigs Hi Patsy, I would love to try the method! Thanks -Liz Liz Wilson Bethyl Labs ________________________________________________________________________ ____ Message: 7 Date: Sat, 10 Apr 2010 10:33:02 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs To: "'Liz Wilson'" , Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up the method at work on Monday and send it to you if you contact me again to remind me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From Harris <@t> medinst.com Mon Apr 12 12:37:13 2010 From: Harris <@t> medinst.com (Corliss Harris) Date: Mon Apr 12 12:37:27 2010 Subject: [Histonet] Cure Mount/ Cure Mount II Message-ID: <4BC32208.9DCF.0062.0@medinst.com> Is anyone familiar with the long term storage of slides that have been mounted using Cure Mount or Cure Mount II by Instrumedics? Are the stains that these mountant medias don't work well? Any comments on this would be great! Thanks! Corliss Harris -- Corliss Harris Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-463-7537 ext. 1150 765-497-0641-fax From cpyse <@t> x-celllab.com Mon Apr 12 14:38:59 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Apr 12 14:39:32 2010 Subject: [Histonet] Thanks Message-ID: <000001cada77$cc50c810$64f25830$@com> Thanks to all for providing me with the information I requested. You have made my decision making so much easier. Thanks again Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From sbreeden <@t> nmda.nmsu.edu Mon Apr 12 14:42:23 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Apr 12 14:42:27 2010 Subject: [Histonet] For Warren Eddings Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47021@nmdamailsvr.nmda.ad.nmsu.edu> Warren, please send me another email address other than the one you just used to request a copy of the Histo Stories. I'm getting an error that will not allow it to be received at your end of the wire. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Ken_Marissael <@t> vwr.com Mon Apr 12 15:01:12 2010 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Mon Apr 12 15:01:22 2010 Subject: [Histonet] Ken Marissael/VWRI is at the VWR Regioanl HealthCare Sales Meeting Message-ID: I will be out of the office starting 04/12/2010 and will not return until 04/16/2010. I will be away beginning Monday 4/12 and will return on Monday 4/19. I will have my Blackberry, and access to e-mail in the evenings. If you need immediate help, please contact Customer Service at 1-800-932-5000. From mark <@t> frozensection.com Tue Apr 13 08:13:22 2010 From: mark <@t> frozensection.com (Mark Roberts) Date: Tue Apr 13 08:13:30 2010 Subject: [Histonet] CD204 (MSR1) antibody that works well Message-ID: <9014773A-5474-42FD-882D-646ECCB042E1@frozensection.com> From: Mark Roberts, Northwestern University Hello to the group: I am looking to find a CD204 (MSR1) antibody for use on human cells by immunohistochemistry on frozen and paraffin sections. Does anyone on the list use a validated, commercially available clone that works well? A small number of companies makes the antibody (Abcam, AbD Serotec, Atlas Antibodies, Cosmo Bio Co., Novus Biologicals and Transgenic, Inc). The original paper that I studied, by J-W Tjiu in Journal of Investigative Dermatology, 2009 used a monoclonal antibody (Mab 2708) from R & D Systems, but I believe that it may be useful only for flow cytometry. Many thanks for your help! MR/PL From GenieJacobs <@t> texashealth.org Tue Apr 13 09:11:49 2010 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Tue Apr 13 09:12:17 2010 Subject: [Histonet] part-time job Message-ID: <7D894CD1537BE943BBF97DEDAFD8B4F454788006@PHDEXMB03.txhealth.org> Part-time Histo/Immuno Job (ASCP) required Using Ventana Ultra Hours neg.11-3pm MDPathology Plano,TX 972-981-3108 The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From kmerriam2003 <@t> yahoo.com Tue Apr 13 09:34:46 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Apr 13 09:34:50 2010 Subject: [Histonet] NSH online registration Message-ID: <19653.99521.qm@web50303.mail.re2.yahoo.com> Anyone else having trouble with the online registration for the meeting in Seattle? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 13 09:38:47 2010 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Apr 13 09:39:00 2010 Subject: [Histonet] Sally Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C5786C58CF@HPEMX3.HealthPartners.int> A big "THANK YOU AND JOB WELL DONE" to Sally Breeden for compiling the stories of histotechs and how they got their start! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From pathology.histology <@t> gmail.com Tue Apr 13 09:38:59 2010 From: pathology.histology <@t> gmail.com (path lab) Date: Tue Apr 13 09:39:04 2010 Subject: [Histonet] position available in Northern Virginia Message-ID: Histology technician position available in busy northern Virginia laboratory. Experience in a high quality/production lab and necropsy helpful but not required. Please reply to pathology.histology@gmail.com Thanks From bbroders <@t> unlnotes.unl.edu Tue Apr 13 10:09:02 2010 From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen) Date: Tue Apr 13 10:09:14 2010 Subject: [Histonet] Nonspecific intranuclear IHC staining Message-ID: >From time to time, we experience nonspecific intranuclear staining on a variety of stains for infectious agents and a variety of tissues and a variety of cases. More specifically, bovine tissues (lung and intestines) using antibodies against BHV-1, rotavirus, and coronavirus. We are using Ventana's Ultra Red kits and have seen it with DAB as well. Anyone else experience this and what did you do about it? Thanks. Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From loftonjt <@t> holycrosshealth.org Tue Apr 13 10:30:48 2010 From: loftonjt <@t> holycrosshealth.org (Jimmy Lofton) Date: Tue Apr 13 10:31:15 2010 Subject: [Histonet] Senior Histology Position Available Message-ID: <4BC455E7.9B70.0056.0@holycrosshealth.org> We have a senior histology position available in the Washington DC area. See information located on the web site. www.holycrosshealth.org Thanks, Mr. Jimmy Lofton Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) loftonjt@holycrosshealth.org Trinity Health MailGate made the following annotations --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. --------------------------------------------------------------------- From rmweber113 <@t> comcast.net Tue Apr 13 10:38:17 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Apr 13 10:38:20 2010 Subject: [Histonet] JOB OPPERTUNITY NJ In-Reply-To: <864213117.16721711271172650371.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <1031616962.16726191271173097207.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Looking for a full-time ASCP certified histologist to run a newly developed state of the art urology laboratory located in Sommerville NJ.?Candidate must have 2 years histology expierence .? Responsibilities include grossing, microwave processing, cutting, embedding, routing and immuno staining as well as cytology prep.? Interested candidates can send? their resume to Coastal Pathology Consulting Services at 267 722-8308 From GenieJacobs <@t> texashealth.org Tue Apr 13 10:52:12 2010 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Tue Apr 13 10:52:32 2010 Subject: [Histonet] FW: part-time job Message-ID: <7D894CD1537BE943BBF97DEDAFD8B4F454788007@PHDEXMB03.txhealth.org> From: Jacobs, Genie Sent: Tuesday, April 13, 2010 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu'; 'histonet@lists.utsouthwestern.edu' Subject: part-time job Part-time Histo/Immuno Job (ASCP) required Using Ventana Ultra Hours neg.11-3pm MDPathology Plano,TX 972-981-3108 The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From alyssa <@t> alliedsearchpartners.com Tue Apr 13 11:20:52 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Apr 13 11:20:59 2010 Subject: [Histonet] Histology/Pathology Jobs (New Jobs Available) Message-ID: Allied Search Partners is currently accepting resumes for the following positions: *All positions eligible for a generous sign on bonus through MPath Search Partners, A division of Allied Search Partners (Agency) and are TBD upon written offer letter* *We are also offering a referral bonus for all positions* *Supervisory & Management:* Pathology Supervisor-Fresno, CA Histology Supervisor-Las Vegas, NV Histology Supervisor-Auburndale, FL (Polk County) * * *Technologist/Technician:* Histotechnician/Histotechnologist-Corpus Christi, TX Histotechnician/Histotechnologist *(Bone Marrow Exp. Needed)-*Clifton, NJ *Mohs* Histotech *(Part Time 2 days/week)*-New York City, NY Histotechnician/Histotechnologist-Las Vegas, NV Histotechnician/Histotechnologist- Northern San Diego, CA Histotechnician/Histotechnologist-Plant City, FL Histotechnician/Histotechnologist-Tavares, FL *Other Pathology Positions:* Pathologist?s Assistant-Las Vegas, NV If interested in any of the above positions please submit resume to Alyssa@alliedsearchpartners.com and state which location and position is of interest. Please be aware that submitting your resume is only for prescreening purposes of our recruiters and no resume will be submitted to our clients without speaking to you initially. Thank you! From shive003 <@t> umn.edu Tue Apr 13 14:24:53 2010 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Tue Apr 13 14:24:58 2010 Subject: [Histonet] RE: CD31 in pigs In-Reply-To: References: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> Message-ID: Me, too, please. Jan Shivers UMN Vet Diag Lab On Apr 12 2010, Liz Wilson wrote: >Hi Patsy, >I would love to try the method! Thanks -Liz > >Liz Wilson >Bethyl Labs > > > ____________________________________________________________________________ >Message: 7 >Date: Sat, 10 Apr 2010 10:33:02 -0600 >From: "Patsy Ruegg" >Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs >To: "'Liz Wilson'" , > >Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> >Content-Type: text/plain; charset="us-ascii" > >A few years ago I spent a lot of effort on using Serotec's mouse >anti-porcine CD31 for ffpe porcine tissue and I did get some positive >results with unusual pretreatment methods, as I recall I had to use a >waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up >the method at work on Monday and send it to you if you contact me again to >remind me. > >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech >12635 Montview Blvd. Ste.215 >Aurora, CO 80045 >720-859-4060 >fax 720-859-4110 >www.ihctech.net >www.ihcrg.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Tue Apr 13 15:31:54 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Apr 13 15:32:16 2010 Subject: [Histonet] RE: CD31 in pigs In-Reply-To: References: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> Message-ID: <905D47581925CEEDA6259D89@CDYwxp1931.ad.med.buffalo.edu> Me three, please. :-) I just discovered we have this same antibody in our lab but my boss was unsuccessful with it (he had tried 95oC for 45 min, pH 6.0). Thanks! Merced --On Tuesday, April 13, 2010 2:24 PM -0500 shive003@umn.edu wrote: > Me, too, please. > > Jan Shivers > UMN Vet Diag Lab > > On Apr 12 2010, Liz Wilson wrote: > >> Hi Patsy, >> I would love to try the method! Thanks -Liz >> >> Liz Wilson >> Bethyl Labs >> >> >> ________________________________________________________________________ >> ____ Message: 7 >> Date: Sat, 10 Apr 2010 10:33:02 -0600 >> From: "Patsy Ruegg" >> Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs >> To: "'Liz Wilson'" , >> >> Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> >> Content-Type: text/plain; charset="us-ascii" >> >> A few years ago I spent a lot of effort on using Serotec's mouse >> anti-porcine CD31 for ffpe porcine tissue and I did get some positive >> results with unusual pretreatment methods, as I recall I had to use a >> waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up >> the method at work on Monday and send it to you if you contact me again >> to remind me. >> >> Patsy >> >> Patsy Ruegg, HT(ASCP)QIHC >> IHCtech >> 12635 Montview Blvd. Ste.215 >> Aurora, CO 80045 >> 720-859-4060 >> fax 720-859-4110 >> www.ihctech.net >> www.ihcrg.org >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From mtighe <@t> trudeauinstitute.org Tue Apr 13 16:01:07 2010 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Apr 13 16:01:26 2010 Subject: [Histonet] Vibrating microtome sections Message-ID: <4BC4A2C6.26E4.00EE.0@trudeauinstitute.org> I would like cut fresh spleen and lymph node sections on a vibrating microtome. I have tried embedding in low melt agarose but the tissue seems to be too flexable to get good repeating sections (uniform thickness). When the knife does catch the cells seem to pour out into the surrounding medium. Does anybody have a suggestion or a reliable method they would be willing to share? Maybe there is a embedding medium that I am not aware of? Thanks for any Help!! Mike From Tom_Wells <@t> bcit.ca Tue Apr 13 17:32:57 2010 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Tue Apr 13 17:34:54 2010 Subject: [Histonet] commercial control slides Message-ID: I wonder if any of you have any experience purchasing control tissues or slides from a commercial site. As a school, we don't have access to tissues as you do when you work in a hospital. While our local hospitals have been very kind in supplying control tissues they need them too so it is not an unlimited supply. Could anyone recommend a company that sells good quality and fairly cheap control materials. I would prefer tissues, but, precut slides are ok as well. Thanks. Tom Tom Wells BSc, ART Faculty School of Medical Laboratory Sciences British Columbia Institute of Technology Burnaby, BC Canada From jcampbell <@t> vdxpathology.com Tue Apr 13 18:46:10 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Tue Apr 13 18:46:14 2010 Subject: [Histonet] studying for ASCP certification exam Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FC2@VDXSERVER01.vdxpathology.local> I am in the process of studying for my HT certification exam and was wondering if anyone had any recommendations on text books or study guides they found to be helpful. I am currently studying "Histotechnology: A Self-Instructional Text", by Carson and just recieved the "BOR study Guide for Histotechnology". Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell From iskaliora <@t> bioacademy.gr Wed Apr 14 00:58:09 2010 From: iskaliora <@t> bioacademy.gr (iskaliora@bioacademy.gr) Date: Wed Apr 14 00:58:21 2010 Subject: [Histonet] RE: CD31 in pigs In-Reply-To: <905D47581925CEEDA6259D89@CDYwxp1931.ad.med.buffalo.edu> References: <20100410170521.8E8C4300064@mx02.mfg.onr.siteprotect.com> <905D47581925CEEDA6259D89@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <53369.92.118.175.186.1271224689.squirrel@webmail.bioacademy.gr> please add me to the growing list as well. thanks, IS. ----------------------------------------------------- Irini Skaliora, PhD Research Assistant Professor Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr > Me three, please. :-) > > I just discovered we have this same antibody in our lab but my boss was > unsuccessful with it (he had tried 95oC for 45 min, pH 6.0). > > Thanks! > > Merced > > --On Tuesday, April 13, 2010 2:24 PM -0500 shive003@umn.edu wrote: > >> Me, too, please. >> >> Jan Shivers >> UMN Vet Diag Lab >> >> On Apr 12 2010, Liz Wilson wrote: >> >>> Hi Patsy, >>> I would love to try the method! Thanks -Liz >>> >>> Liz Wilson >>> Bethyl Labs >>> >>> >>> ________________________________________________________________________ >>> ____ Message: 7 >>> Date: Sat, 10 Apr 2010 10:33:02 -0600 >>> From: "Patsy Ruegg" >>> Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs >>> To: "'Liz Wilson'" , >>> >>> Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> A few years ago I spent a lot of effort on using Serotec's mouse >>> anti-porcine CD31 for ffpe porcine tissue and I did get some positive >>> results with unusual pretreatment methods, as I recall I had to use a >>> waterbath for hier set at 70dc for 3 hours in hph buffer. I will look >>> up >>> the method at work on Monday and send it to you if you contact me again >>> to remind me. >>> >>> Patsy >>> >>> Patsy Ruegg, HT(ASCP)QIHC >>> IHCtech >>> 12635 Montview Blvd. Ste.215 >>> Aurora, CO 80045 >>> 720-859-4060 >>> fax 720-859-4110 >>> www.ihctech.net >>> www.ihcrg.org >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joost.bruijntjes <@t> tno.nl Wed Apr 14 01:04:28 2010 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Wed Apr 14 01:04:19 2010 Subject: FW: [Histonet] RE: CD31 in pigs Message-ID: <8865601DD17A554CB489C17FFD8A51B20369D0EB@MAIL04.tsn.tno.nl> And another one, thanks Joost TNO Quality of Life The Netherlands -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shive003@umn.edu Sent: dinsdag 13 april 2010 21:25 To: Liz Wilson Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: CD31 in pigs Me, too, please. Jan Shivers UMN Vet Diag Lab On Apr 12 2010, Liz Wilson wrote: >Hi Patsy, >I would love to try the method! Thanks -Liz > >Liz Wilson >Bethyl Labs > > > ________________________________________________________________________ ____ >Message: 7 >Date: Sat, 10 Apr 2010 10:33:02 -0600 >From: "Patsy Ruegg" >Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs >To: "'Liz Wilson'" , > >Message-ID: <59ADBA33D53B44778565F88CBD7FA13A@prueggihctechlt> >Content-Type: text/plain; charset="us-ascii" > >A few years ago I spent a lot of effort on using Serotec's mouse >anti-porcine CD31 for ffpe porcine tissue and I did get some positive >results with unusual pretreatment methods, as I recall I had to use a >waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up >the method at work on Monday and send it to you if you contact me again to >remind me. > >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech >12635 Montview Blvd. Ste.215 >Aurora, CO 80045 >720-859-4060 >fax 720-859-4110 >www.ihctech.net >www.ihcrg.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From W.E.J.Hoekert <@t> olvg.nl Wed Apr 14 07:39:38 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Wed Apr 14 07:39:51 2010 Subject: [Histonet] Smad4 on a Ventana Benchmark XT Message-ID: <1190CB05C44B13409483514729C2FC360C0AB9@PAIT42.olvg.nl> Hi Histonetters, We are thinking of taking the Ventana Benchmark XT, and I am testing it right now. So far, it is impossible to get the Smad4 up and running. I have tried everything: -Pretreatment with cc1 and cc2 for up to 90 minutes -Incubation of primairy AB for up to 90 minutes -Incubation at 37 degrees and room temperature -Pretreatment using a PT module, and even with a microwave -Increasing the concentration of AB But nothing works. Maybe I should try another clone? Right now we are using clone B-8 from Santa Cruz, and we are using HIER (EDTA) as pretreatment. Does anyone have any suggestions? I hope to hear from you. Willem Hoekert OLVG, PA Lab The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From kelleydurden <@t> pathology.ufl.edu Wed Apr 14 07:51:19 2010 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Wed Apr 14 07:51:31 2010 Subject: [Histonet] Studying for HT Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE5A62E84C@HSC-CMS01.ad.ufl.edu> I felt like the most important part and the majority of the test came from information about special stains. I think the last time I looked special stains made up about 70% of the test. So definitely know the information but very importantly know the "color plates" from the books you are using to study. I prefer the Carson book you already mentioned, the 1st and the 2nd edition are always good to have, and the original AFIP manual - the green one. Good luck! Kelley HT(ASCP) From mpence <@t> grhs.net Wed Apr 14 08:17:49 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 14 08:17:54 2010 Subject: [Histonet] studying for ASCP certification exam In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8181FC2@VDXSERVER01.vdxpathology.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DC2@is-e2k3.grhs.net> Also try: "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. I have heard this is a VERY hard book to get a hold of these days and if you can it will cost! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Tuesday, April 13, 2010 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] studying for ASCP certification exam I am in the process of studying for my HT certification exam and was wondering if anyone had any recommendations on text books or study guides they found to be helpful. I am currently studying "Histotechnology: A Self-Instructional Text", by Carson and just recieved the "BOR study Guide for Histotechnology". Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Wed Apr 14 08:58:12 2010 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Apr 14 09:01:36 2010 Subject: FW: [Histonet] studying for ASCP certification exam Message-ID: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> Jennifer, HISTOLOGICAL AND HISTOCHEMICAL METHODS. 4th Ed. Kiernan, J.A. In my opinion this is the best histotechnique text currently available. Amazon UK has it in stock for ?34.99 and for a text of this quality it's a bargain. If you are studying for an exam this text gives everything you'll need, but more importantly, it is written in a style that is easily understood. Better still, when you have finished studying, the text can sit on a shelf in your lab and become your standard lab manual. No tie in with the author or publisher but I have 2 copies, one in my office and the other in the lab. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: 14 April 2010 14:18 To: Jennifer Campbell; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] studying for ASCP certification exam Also try: "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. I have heard this is a VERY hard book to get a hold of these days and if you can it will cost! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Tuesday, April 13, 2010 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] studying for ASCP certification exam I am in the process of studying for my HT certification exam and was wondering if anyone had any recommendations on text books or study guides they found to be helpful. I am currently studying "Histotechnology: A Self-Instructional Text", by Carson and just recieved the "BOR study Guide for Histotechnology". Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Apr 14 10:09:56 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Apr 14 10:10:02 2010 Subject: [Histonet] Communication when pathologist are not in same area as lab Message-ID: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> Hello to all in histoland. What are you doing to minimize the communication breakdown, when the pathologist are not housed in the lab. We will be moving to a new lab and the pathologist will not be in the new bldg with us. They will be in the old building next door. Are you relying upon email and phone interactions. My directors concern is how will this effect the flow of information, especially when they are used to face to face interactions. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From sbreeden <@t> nmda.nmsu.edu Wed Apr 14 10:19:18 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Apr 14 10:19:22 2010 Subject: [Histonet] Communication when pathologist are not in same area aslab In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47036@nmdamailsvr.nmda.ad.nmsu.edu> "Communication"? I'm just down the HALL and we don't communicate! :) Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From foreightl <@t> gmail.com Wed Apr 14 11:02:21 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Wed Apr 14 11:02:31 2010 Subject: FW: [Histonet] studying for ASCP certification exam In-Reply-To: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> References: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> Message-ID: I definetly agree with the previous suggestions. Those 3 books are in the top 5 of my collections. Another book that might help you because of the illustrations (special stain color pictures on the test!) is: Theory and Practice of Histological Techniques 5th edition edited by Bancroft and Gamble Unfortunately it is rather pricey. Another good standby because so many books seem to cite it (classic): AFIP Laboratory Methods in Histotechnology latest edition is Prophet et.al. ISBN: 1-881041-00-X Good luck with your test! On Wed, Apr 14, 2010 at 6:58 AM, Ian Montgomery < ian.montgomery@bio.gla.ac.uk> wrote: > Jennifer, > > HISTOLOGICAL AND HISTOCHEMICAL METHODS. 4th Ed. Kiernan, J.A. > > In my opinion this is the best histotechnique text currently > available. Amazon UK has it in stock for ?34.99 and for a text of this > quality it's a bargain. If you are studying for an exam this text gives > everything you'll need, but more importantly, it is written in a style that > is easily understood. Better still, when you have finished studying, the > text can sit on a shelf in your lab and become your standard lab manual. No > tie in with the author or publisher but I have 2 copies, one in my office > and the other in the lab. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > Glasgow, > G12 8QQ. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike > Pence > Sent: 14 April 2010 14:18 > To: Jennifer Campbell; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] studying for ASCP certification exam > > Also try: > > "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. > > I have heard this is a VERY hard book to get a hold of these days and if > you can it will cost! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > Campbell > Sent: Tuesday, April 13, 2010 6:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] studying for ASCP certification exam > > > I am in the process of studying for my HT certification exam and was > wondering if anyone had any recommendations on text books or study > guides they found to be helpful. I am currently studying > "Histotechnology: A Self-Instructional Text", by Carson and just > recieved the "BOR study Guide for Histotechnology". Are there any other > sources you would recommend? I have taken a look at the suggested > reading list on the ASCP website but, there are quite a few books > listed. > > Thanks in advance, > > Jennifer Campbell _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From tjasper <@t> copc.net Wed Apr 14 11:32:14 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Apr 14 11:32:21 2010 Subject: [Histonet] studying for ASCP certification exam References: <5658CBDB9EAE6545ABE50D2563D81BF8181FC2@VDXSERVER01.vdxpathology.local> Message-ID: <90354A475B420441B2A0396E5008D49695E889@copc-sbs.COPC.local> Jennifer, I think all the suggestions you've gotten so far are good. I'd add - The Theory and Practice of Histological Technique - by Bancroft and Stevens. I also believe there are NSH study materials available, maybe the BOR guide you mentioned is the same thing, not sure. Good luck! Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/617-2831 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Tuesday, April 13, 2010 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] studying for ASCP certification exam I am in the process of studying for my HT certification exam and was wondering if anyone had any recommendations on text books or study guides they found to be helpful. I am currently studying "Histotechnology: A Self-Instructional Text", by Carson and just recieved the "BOR study Guide for Histotechnology". Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Apr 14 11:54:22 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 14 11:55:04 2010 Subject: [Histonet] ms For all those looking for this info from me. I use the serotech mouse anti porcine CD31 on pig tonsil at 1/10-1/50 overnight after HIER in a waterbath set at 80dc for 3 hours with prewarmed citrate buffer ph6. I use this with an anti mouse labeled polymer detection system, either hrp/dab or alk.phos./red. You can join the group online at www.ihcrg.org This one is tuff, but this is what worked for us. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From kimtournear <@t> yahoo.com Wed Apr 14 12:22:01 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Apr 14 12:22:05 2010 Subject: [Histonet] Communication when pathologist are not in same area as lab In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> Message-ID: <219954.26751.qm@web54202.mail.re2.yahoo.com> I don't think my pathologist's are aware they have access to email...LOL...as for communication, they are in another world. ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Wed, 4/14/10, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Communication when pathologist are not in same area as lab To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 14, 2010, 8:09 AM Hello to all in histoland.? What are you doing to minimize the communication breakdown, when the pathologist are not housed in the lab. We will be moving to a new lab and the pathologist will not be in the new bldg with us.? They will be in the old building next door. Are you relying upon email and phone interactions.???My directors concern is how will this effect the flow of information, especially when they are used to face to face interactions. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Wed Apr 14 12:27:31 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Apr 14 12:27:38 2010 Subject: [Histonet] Communication when pathologist are not in same In-Reply-To: <20100414170320.699DA12678C@mail.pa-ucl.com> References: <20100414170320.699DA12678C@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC0644165A2B29A@hestia.ad.pa-ucl.com> Hi Allison- We rely on email, phone and tracking forms. Anytime we encounter a problem with a slide/block/number etc., we fill out a tracking form and send with the case. The pathologist of record then responds and sends it back to us in histology. We have had good success with this, and it's also a good tool for tracking problems/trends. Our histology lab is in a central location with no pathologist present as they are at two hospital sites in our city. It also helps that we have a great group of pathologists to work with. Nancy Schmitt, HT(ASCP),MLT(CSMLS) Histology Coordinator _________________________________________________- Date: Wed, 14 Apr 2010 10:09:56 -0500 From: "Scott, Allison D" Subject: To: Message-ID: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. What are you doing to minimize the communication breakdown, when the pathologist are not housed in the lab. We will be moving to a new lab and the pathologist will not be in the new bldg with us. They will be in the old building next door. Are you relying upon email and phone interactions. My directors concern is how will this effect the flow of information, especially when they are used to face to face interactions. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From kmerriam2003 <@t> yahoo.com Wed Apr 14 12:32:53 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Apr 14 12:32:57 2010 Subject: FW: [Histonet] studying for ASCP certification exam In-Reply-To: References: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> Message-ID: <277762.83491.qm@web50306.mail.re2.yahoo.com> The NSH?sells a set of study guides?on histology (maybe 10 of them, on fixation, various special stain categories and the like), I think you will find them quite helpful to see how much you actually remember after reading the textbooks. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Pat Laurie To: histonet@lists.utsouthwestern.edu Sent: Wed, April 14, 2010 12:02:21 PM Subject: Re: FW: [Histonet] studying for ASCP certification exam I definetly agree with the previous suggestions.? Those 3 books are in the top 5 of my collections.? Another book that might help you because of the illustrations (special stain color pictures on the test!) is: Theory and Practice of Histological Techniques 5th edition edited by Bancroft and Gamble Unfortunately it is rather pricey. Another good standby because so many books seem to cite it (classic): AFIP Laboratory Methods in Histotechnology latest edition is Prophet et.al. ISBN: 1-881041-00-X Good luck with your test! On Wed, Apr 14, 2010 at 6:58 AM, Ian Montgomery < ian.montgomery@bio.gla.ac.uk> wrote: > Jennifer, > > HISTOLOGICAL AND HISTOCHEMICAL METHODS. 4th Ed. Kiernan, J.A. > >? ? ? ? In my opinion this is the best histotechnique text currently > available. Amazon UK has it in stock for ?34.99 and for a text of this > quality it's a bargain. If you are studying for an exam this text gives > everything you'll need, but more importantly, it is written in a style that > is easily understood. Better still, when you have finished studying, the > text can sit on a shelf in your lab and become your standard lab manual. No > tie in with the author or publisher but I have 2 copies, one in my office > and the other in the lab. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > Glasgow, > G12 8QQ. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu >? [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike > Pence > Sent: 14 April 2010 14:18 > To: Jennifer Campbell; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] studying for ASCP certification exam > > Also try: > > "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. > > I have heard this is a VERY hard book to get a hold of these days and if > you can it will cost! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > Campbell > Sent: Tuesday, April 13, 2010 6:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] studying for ASCP certification exam > > >? I am in the process of studying for my HT certification exam and was > wondering if anyone had any recommendations on text books or study > guides they found to be helpful.? I am currently studying > "Histotechnology: A Self-Instructional Text", by Carson and just > recieved the "BOR study Guide for Histotechnology".? Are there any other > sources you would recommend?? I have taken a look at the suggested > reading list on the ASCP website but, there are quite a few books > listed. > > Thanks in advance, > > Jennifer Campbell _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Apr 14 12:37:06 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 14 12:37:49 2010 Subject: [Histonet] formalex Message-ID: <99F444104E8D4F63BC9CBCBB80BEC2CC@Patsyoffice> Does anyone know what is in Formalex formalin neutralizer? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From JWeems <@t> sjha.org Wed Apr 14 12:40:47 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 14 12:40:52 2010 Subject: FW: [Histonet] studying for ASCP certification exam In-Reply-To: <277762.83491.qm@web50306.mail.re2.yahoo.com> References: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> <277762.83491.qm@web50306.mail.re2.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015D426096@CHEXCMS10.one.ads.che.org> I would suggest Freida Carson's books and work book and computer tests (if still available) and the NSH study guides. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, April 14, 2010 13:33 To: Pat Laurie; histonet@lists.utsouthwestern.edu Subject: Re: FW: [Histonet] studying for ASCP certification exam The NSH?sells a set of study guides?on histology (maybe 10 of them, on fixation, various special stain categories and the like), I think you will find them quite helpful to see how much you actually remember after reading the textbooks. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Pat Laurie To: histonet@lists.utsouthwestern.edu Sent: Wed, April 14, 2010 12:02:21 PM Subject: Re: FW: [Histonet] studying for ASCP certification exam I definetly agree with the previous suggestions.? Those 3 books are in the top 5 of my collections.? Another book that might help you because of the illustrations (special stain color pictures on the test!) is: Theory and Practice of Histological Techniques 5th edition edited by Bancroft and Gamble Unfortunately it is rather pricey. Another good standby because so many books seem to cite it (classic): AFIP Laboratory Methods in Histotechnology latest edition is Prophet et.al. ISBN: 1-881041-00-X Good luck with your test! On Wed, Apr 14, 2010 at 6:58 AM, Ian Montgomery < ian.montgomery@bio.gla.ac.uk> wrote: > Jennifer, > > HISTOLOGICAL AND HISTOCHEMICAL METHODS. 4th Ed. Kiernan, J.A. > >? ? ? ? In my opinion this is the best histotechnique text currently >available. Amazon UK has it in stock for ?34.99 and for a text of this >quality it's a bargain. If you are studying for an exam this text gives >everything you'll need, but more importantly, it is written in a style >that is easily understood. Better still, when you have finished >studying, the text can sit on a shelf in your lab and become your >standard lab manual. No tie in with the author or publisher but I have >2 copies, one in my office and the other in the lab. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > Glasgow, > G12 8QQ. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu >? [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike >Pence > Sent: 14 April 2010 14:18 > To: Jennifer Campbell; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] studying for ASCP certification exam > > Also try: > > "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. > > I have heard this is a VERY hard book to get a hold of these days and > if you can it will cost! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Jennifer Campbell > Sent: Tuesday, April 13, 2010 6:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] studying for ASCP certification exam > > >? I am in the process of studying for my HT certification exam and was >wondering if anyone had any recommendations on text books or study >guides they found to be helpful.? I am currently studying > "Histotechnology: A Self-Instructional Text", by Carson and just >recieved the "BOR study Guide for Histotechnology".? Are there any >other sources you would recommend?? I have taken a look at the >suggested reading list on the ASCP website but, there are quite a few >books listed. > > Thanks in advance, > > Jennifer Campbell _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From malbenatti <@t> googlemail.com Wed Apr 14 12:43:43 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Apr 14 12:43:51 2010 Subject: FW: [Histonet] studying for ASCP certification exam In-Reply-To: <277762.83491.qm@web50306.mail.re2.yahoo.com> References: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> <277762.83491.qm@web50306.mail.re2.yahoo.com> Message-ID: Theory and Practice of Histological Techniques by John D. Bancroft and Marilyn Gamble Dr. you can probably get hold of a copy on Amazon for 1/2 price, even at full price it definitely worth every cents. On Wed, Apr 14, 2010 at 6:32 PM, Kim Merriam wrote: > The NSH sells a set of study guides on histology (maybe 10 of them, on > fixation, various special stain categories and the like), I think you will > find them quite helpful to see how much you actually remember after reading > the textbooks. > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > > ________________________________ > From: Pat Laurie > To: histonet@lists.utsouthwestern.edu > Sent: Wed, April 14, 2010 12:02:21 PM > Subject: Re: FW: [Histonet] studying for ASCP certification exam > > I definetly agree with the previous suggestions. Those 3 books are in the > top 5 of my collections. Another book that might help you because of the > illustrations (special stain color pictures on the test!) is: > > Theory and Practice of Histological Techniques > 5th edition edited by Bancroft and Gamble > > Unfortunately it is rather pricey. > > > Another good standby because so many books seem to cite it (classic): > > AFIP Laboratory Methods in Histotechnology > latest edition is Prophet et.al. ISBN: 1-881041-00-X > > Good luck with your test! > On Wed, Apr 14, 2010 at 6:58 AM, Ian Montgomery < > ian.montgomery@bio.gla.ac.uk> wrote: > > > Jennifer, > > > > HISTOLOGICAL AND HISTOCHEMICAL METHODS. 4th Ed. Kiernan, J.A. > > > > In my opinion this is the best histotechnique text currently > > available. Amazon UK has it in stock for ?34.99 and for a text of this > > quality it's a bargain. If you are studying for an exam this text gives > > everything you'll need, but more importantly, it is written in a style > that > > is easily understood. Better still, when you have finished studying, the > > text can sit on a shelf in your lab and become your standard lab manual. > No > > tie in with the author or publisher but I have 2 copies, one in my office > > and the other in the lab. > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike > > Pence > > Sent: 14 April 2010 14:18 > > To: Jennifer Campbell; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] studying for ASCP certification exam > > > > Also try: > > > > "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. > > > > I have heard this is a VERY hard book to get a hold of these days and if > > you can it will cost! > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > > Campbell > > Sent: Tuesday, April 13, 2010 6:46 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] studying for ASCP certification exam > > > > > > I am in the process of studying for my HT certification exam and was > > wondering if anyone had any recommendations on text books or study > > guides they found to be helpful. I am currently studying > > "Histotechnology: A Self-Instructional Text", by Carson and just > > recieved the "BOR study Guide for Histotechnology". Are there any other > > sources you would recommend? I have taken a look at the suggested > > reading list on the ASCP website but, there are quite a few books > > listed. > > > > Thanks in advance, > > > > Jennifer Campbell _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > PH: 206-215-5949 > plaurie@cellnetix.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From pedro.louro <@t> spcorp.com Wed Apr 14 12:53:03 2010 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Wed Apr 14 12:53:08 2010 Subject: [Histonet] Region II Meeting Registration - June 10-12 - Atlantic City, NJ Message-ID: > Anyone around the Atlantic City area on June 10-12? > The State Societies of NJ, PA, DE, MD and VA invite you to attend the > Region II meeting. > > Registration is now open. > Please click on the link below to view the flyer in pdf format. > http://www.keepandshare.com/doc/1857485/2010regioniimeetingbrochure-pdf- april-14-2010-1-38-pm-572k?da=y > See you there, > Pedro Louro, MBA, QIHC Vice President and Co-Chair Membership Committee New Jersey Society for Histotechnology (NJSH) Ph. 908-473-4501 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From sbreeden <@t> nmda.nmsu.edu Wed Apr 14 13:06:55 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Apr 14 13:11:51 2010 Subject: [Histonet] Storage Boxes Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4703D@nmdamailsvr.nmda.ad.nmsu.edu> One of my former labs used plastic stacking boxes for holding blocks between cutting and filing, as "tool" trays and for many other purposes. These are the same boxes used in eyeglass fabricators (LensCrafters, etc.). I've been searching for them for quite some time and have just found a supplier at www.westernoptical.com (and likely other optical suppliers as well) - search for "plastic stackable shop trays". I just bought a dozen for $30. I find them indispensible, which is why I've been looking for them for so long. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From disbrc <@t> shands.ufl.edu Wed Apr 14 14:28:00 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Wed Apr 14 14:28:08 2010 Subject: [Histonet] list responses Message-ID: <4BC5DEFE.72AC.0059.1@shands.ufl.edu> Hi We kept wondering why our responses to the discussion are not seen on the daily list. We then realized the response must be going directly to the person who wrote the original post. Is there anyway the responses can be seen on the list as we are losing the input of the others. Thanks, Carrie From anonwums1 <@t> gmail.com Wed Apr 14 14:30:00 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Wed Apr 14 14:31:23 2010 Subject: [Histonet] list responses In-Reply-To: <4BC5DEFE.72AC.0059.1@shands.ufl.edu> References: <4BC5DEFE.72AC.0059.1@shands.ufl.edu> Message-ID: Rather than doing a simple reply, choose reply all. Adam On Wed, Apr 14, 2010 at 2:28 PM, Carrie Disbrow wrote: > Hi > We kept wondering why our responses to the discussion are not seen on the > daily list. We then realized the response must be going directly to the > person who wrote the original post. Is there anyway the responses can be > seen on the list as we are losing the input of the others. > Thanks, > Carrie > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From disbrc <@t> shands.ufl.edu Wed Apr 14 14:32:23 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Wed Apr 14 14:32:27 2010 Subject: [Histonet] HTL exam Message-ID: <4BC5E005.72AC.0059.1@shands.ufl.edu> Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what the percentage of enzyme histochemistry, electron microscopy, and cytology questions are for the HTL? Thanks, Carrie From disbrc <@t> shands.ufl.edu Wed Apr 14 14:33:51 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Wed Apr 14 14:33:56 2010 Subject: [Histonet] list responses In-Reply-To: References: <4BC5DEFE.72AC.0059.1@shands.ufl.edu> Message-ID: <4BC5E05D.72AC.0059.1@shands.ufl.edu> Hi Adam, thanks for responding so quickly. Carrie From malbenatti <@t> googlemail.com Wed Apr 14 14:37:33 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Apr 14 14:37:40 2010 Subject: [Histonet] Communication when pathologist are not in same area as lab In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> Message-ID: Well our 5 pathologists, advance practitioner and SPR are just across the way from us, until last July all request were made personally by pathologist to staff and if there were any query with regard to the request made it was sorted then and then and it was pretty straight forward. But the good all days of face to face communication changed when we started to get requests via email, although a good idea, in principle, requests via email are not without pitfalls and therefore face to face communication between lab staff and pathologist is still necessary. say email request can be problematic as very often request. On Wed, Apr 14, 2010 at 4:09 PM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. What are you doing to minimize the > communication breakdown, when the pathologist are not housed in the lab. > We will be moving to a new lab and the pathologist will not be in the > new bldg with us. They will be in the old building next door. Are you > relying upon email and phone interactions. My directors concern is how > will this effect the flow of information, especially when they are used > to face to face interactions. > > Allison Scott HT(ASCP) > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " From Janice.Mahoney <@t> alegent.org Wed Apr 14 14:47:29 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Apr 14 14:47:38 2010 Subject: [Histonet] Communication when pathologist are not in same area as lab In-Reply-To: References: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0C0@EXCHMBC2.ad.ah.local> With the right LIS and standard work you may never have to or get to, depending on your perspective, speak with a Pathologist again. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Wednesday, April 14, 2010 2:38 PM To: Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Communication when pathologist are not in same area as lab Well our 5 pathologists, advance practitioner and SPR are just across the way from us, until last July all request were made personally by pathologist to staff and if there were any query with regard to the request made it was sorted then and then and it was pretty straight forward. But the good all days of face to face communication changed when we started to get requests via email, although a good idea, in principle, requests via email are not without pitfalls and therefore face to face communication between lab staff and pathologist is still necessary. say email request can be problematic as very often request. On Wed, Apr 14, 2010 at 4:09 PM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. What are you doing to minimize the > communication breakdown, when the pathologist are not housed in the lab. > We will be moving to a new lab and the pathologist will not be in the > new bldg with us. They will be in the old building next door. Are you > relying upon email and phone interactions. My directors concern is how > will this effect the flow of information, especially when they are used > to face to face interactions. > > Allison Scott HT(ASCP) > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From tpodawiltz <@t> lrgh.org Wed Apr 14 14:53:46 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Apr 14 14:53:51 2010 Subject: [Histonet] HTL exam In-Reply-To: <4BC5E005.72AC.0059.1@shands.ufl.edu> References: <4BC5E005.72AC.0059.1@shands.ufl.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF82BD@LRGHEXVS1.practice.lrgh.org> I believe you can down load that from the ASCP site. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Wednesday, April 14, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what the percentage of enzyme histochemistry, electron microscopy, and cytology questions are for the HTL? Thanks, Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From jcampbell <@t> vdxpathology.com Wed Apr 14 15:04:14 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Wed Apr 14 15:04:19 2010 Subject: [Histonet] Thank you everyone! Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8386AD9@VDXSERVER01.vdxpathology.local> Thank you for all of those who were so helpful in suggesting reading material my HT exam! I appreciate all of your input and kind words of encouragement. Wish me luck! Jennifer Campbell From malbenatti <@t> googlemail.com Wed Apr 14 15:07:55 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Apr 14 15:08:01 2010 Subject: [Histonet] Communication when pathologist are not in same area as lab In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0C0@EXCHMBC2.ad.ah.local> References: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0C0@EXCHMBC2.ad.ah.local> Message-ID: Oops it's looking like I hit the send key too fast ;) Email request can be problematic as very often request are received after hours. But if you give me the choice between email request and face to face communication, like you mentioned Jan it definitely depend on the individuals involved. M On Wed, Apr 14, 2010 at 8:47 PM, Mahoney,Janice A < Janice.Mahoney@alegent.org> wrote: > With the right LIS and standard work you may never have to or get to, > depending on your perspective, speak with a Pathologist again. > Jan > Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti > Sent: Wednesday, April 14, 2010 2:38 PM > To: Scott, Allison D > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Communication when pathologist are not in same area > as lab > > Well our 5 pathologists, advance practitioner and SPR are just across the > way from us, until last July all request were made personally by > pathologist > to staff and if there were any query with regard to the request made it was > sorted then and then and it was pretty straight forward. > > But the good all days of face to face communication changed when we started > to get requests via email, although a good idea, in principle, requests via > email are not without pitfalls and therefore face to face communication > between lab staff and pathologist is still necessary. > > > On Wed, Apr 14, 2010 at 4:09 PM, Scott, Allison D < > Allison_Scott@hchd.tmc.edu> wrote: > > > Hello to all in histoland. What are you doing to minimize the > > communication breakdown, when the pathologist are not housed in the lab. > > We will be moving to a new lab and the pathologist will not be in the > > new bldg with us. They will be in the old building next door. Are you > > relying upon email and phone interactions. My directors concern is how > > will this effect the flow of information, especially when they are used > > to face to face interactions. > > > > Allison Scott HT(ASCP) > > LBJ Hospital > > 5656 Kelley > > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > > If you have received this e-mail in error, please immediately notify the > > sender by return e-mail and delete this e-mail and any attachments from > > your computer system. > > > > To the extent the information in this e-mail and any attachments contain > > protected health information as defined by the Health Insurance > Portability > > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 > and > > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > > and/or > > privileged. This e-mail may also be confidential and/or privileged under > > Texas law. The e-mail is for the use of only the individual or entity > > named > > above. If you are not the intended recipient, or any authorized > > representative of the intended recipient, you are hereby notified that > any > > review, dissemination or copying of this e-mail and its attachments is > > strictly prohibited. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > " Smile .... it confuses people " > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, > Alegent Health is faithful to the healing ministry of Jesus Christ, > providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly > prohibited and may be unlawful. If you received this communication in > error, please inform us of the erroneous delivery by return e-mail message > from your computer. Additionally, although all attachments have been > scanned at the source for viruses, the recipient should check any > attachments for the presence of viruses before opening. Alegent Health > accepts no liability for any damage caused by any virus transmitted by this > e-mail. Thank you for your cooperation. > > -- " Smile .... it confuses people " From rfields <@t> gidocs.net Wed Apr 14 15:29:34 2010 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Apr 14 15:30:02 2010 Subject: FW: [Histonet] studying for ASCP certification exam References: <6A3145CE9F1E4FC5B2084F951BB5912A@IBLS.GLA.AC.UK> <277762.83491.qm@web50306.mail.re2.yahoo.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B775@GIEXCHANGE.gidocs.net> You can get these all on a CD and take the practice tests as many times as you like. It's a great resource to test yourself. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, April 14, 2010 12:33 PM To: Pat Laurie; histonet@lists.utsouthwestern.edu Subject: Re: FW: [Histonet] studying for ASCP certification exam The NSH?sells a set of study guides?on histology (maybe 10 of them, on fixation, various special stain categories and the like), I think you will find them quite helpful to see how much you actually remember after reading the textbooks. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Pat Laurie To: histonet@lists.utsouthwestern.edu Sent: Wed, April 14, 2010 12:02:21 PM Subject: Re: FW: [Histonet] studying for ASCP certification exam I definetly agree with the previous suggestions.? Those 3 books are in the top 5 of my collections.? Another book that might help you because of the illustrations (special stain color pictures on the test!) is: Theory and Practice of Histological Techniques 5th edition edited by Bancroft and Gamble Unfortunately it is rather pricey. Another good standby because so many books seem to cite it (classic): AFIP Laboratory Methods in Histotechnology latest edition is Prophet et.al. ISBN: 1-881041-00-X Good luck with your test! On Wed, Apr 14, 2010 at 6:58 AM, Ian Montgomery < ian.montgomery@bio.gla.ac.uk> wrote: > Jennifer, > > HISTOLOGICAL AND HISTOCHEMICAL METHODS. 4th Ed. Kiernan, J.A. > >? ? ? ? In my opinion this is the best histotechnique text currently > available. Amazon UK has it in stock for ?34.99 and for a text of this > quality it's a bargain. If you are studying for an exam this text gives > everything you'll need, but more importantly, it is written in a style that > is easily understood. Better still, when you have finished studying, the > text can sit on a shelf in your lab and become your standard lab manual. No > tie in with the author or publisher but I have 2 copies, one in my office > and the other in the lab. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > Glasgow, > G12 8QQ. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu >? [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike > Pence > Sent: 14 April 2010 14:18 > To: Jennifer Campbell; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] studying for ASCP certification exam > > Also try: > > "Theory and Practice of Histotechnology" by Sheehan and Hrapchak. > > I have heard this is a VERY hard book to get a hold of these days and if > you can it will cost! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > Campbell > Sent: Tuesday, April 13, 2010 6:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] studying for ASCP certification exam > > >? I am in the process of studying for my HT certification exam and was > wondering if anyone had any recommendations on text books or study > guides they found to be helpful.? I am currently studying > "Histotechnology: A Self-Instructional Text", by Carson and just > recieved the "BOR study Guide for Histotechnology".? Are there any other > sources you would recommend?? I have taken a look at the suggested > reading list on the ASCP website but, there are quite a few books > listed. > > Thanks in advance, > > Jennifer Campbell _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Wed Apr 14 15:43:04 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Apr 14 15:43:11 2010 Subject: [Histonet] Need your referrals: Seeking an AMAZING histology lab aid Message-ID: <900565.41274.qm@web50904.mail.re2.yahoo.com> Do you know someone you like who really deserves a great job?? Feel free to pass this on: ? My lab team ROCKS and we need more help.? We are seeking a new specialty lab aid to join our crew.? There is plenty of work, we work hard and turn out a beautiful product: we?share the load.? ? If you're looking for job satisfaction, please apply. If you stay in a job for years, please apply. If you have no idea what 'it's not my job' means, please apply. If you want a job that makes you use your brain, please apply. If you?like a job that leaves you a little?tired and still?happy, please apply. If you LOVE making a difference, please apply!! ? Send me your resume and be prepared to fill out an online application and pre-employment assessment test.? We'll teach you the rest.? Opportunity for growth and learning new skills is available for the right candidate.? ? Cheryl tkngflght@yahoo.com ? From a.thotakura <@t> imperial.ac.uk Wed Apr 14 15:46:04 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Wed Apr 14 15:46:38 2010 Subject: [Histonet] IHC mouse antibody on mouse tissue Message-ID: Hi All, Does any one got a nice protocol to do IHC using mouse monoclonal antibody on mouse tissue. Either frozen or paraffin section. Please let me know. Thanks in advance. ANIL From mike <@t> pathview.com Wed Apr 14 15:52:49 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Apr 14 15:53:31 2010 Subject: [Histonet] Communication when pathologist are not in same area as lab In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0C0@EXCHMBC2.ad.ah.local> References: <1872B4A455B7974391609AD8034C79FC8BD742@LBEXCH01.hchd.local> <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0C0@EXCHMBC2.ad.ah.local> Message-ID: <06fd01cadc14$7a2ee230$6e8ca690$@com> Thank you for heading in that direction. The 'right' LIS makes a BIG difference. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, April 14, 2010 2:47 PM To: 'malbenatti@gmail.com'; Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Communication when pathologist are not in same area as lab With the right LIS and standard work you may never have to or get to, depending on your perspective, speak with a Pathologist again. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Wednesday, April 14, 2010 2:38 PM To: Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Communication when pathologist are not in same area as lab Well our 5 pathologists, advance practitioner and SPR are just across the way from us, until last July all request were made personally by pathologist to staff and if there were any query with regard to the request made it was sorted then and then and it was pretty straight forward. But the good all days of face to face communication changed when we started to get requests via email, although a good idea, in principle, requests via email are not without pitfalls and therefore face to face communication between lab staff and pathologist is still necessary. say email request can be problematic as very often request. On Wed, Apr 14, 2010 at 4:09 PM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. What are you doing to minimize the > communication breakdown, when the pathologist are not housed in the lab. > We will be moving to a new lab and the pathologist will not be in the > new bldg with us. They will be in the old building next door. Are you > relying upon email and phone interactions. My directors concern is how > will this effect the flow of information, especially when they are used > to face to face interactions. > > Allison Scott HT(ASCP) > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Apr 14 16:54:51 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Apr 14 16:54:54 2010 Subject: [Histonet] Communication when pathologist are not in sameareaas lab Message-ID: <010801cadc1d$14bef5d5$0201a8c0@yumaregional.local> I agree with Janice processes need to be defined and then look at the right technology to implement. This is were LIS plays a key role. But I would warn you that the LIS company needs to be forward thinking and open to ingtergration with vendors. Just my thoughts. Michael Mihalik wrote: Thank you for heading in that direction. The 'right' LIS makes a BIG difference. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, April 14, 2010 2:47 PM To: 'malbenatti@gmail.com'; Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Communication when pathologist are not in same area as lab With the right LIS and standard work you may never have to or get to, depending on your perspective, speak with a Pathologist again. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Wednesday, April 14, 2010 2:38 PM To: Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Communication when pathologist are not in same area as lab Well our 5 pathologists, advance practitioner and SPR are just across the way from us, until last July all request were made personally by pathologist to staff and if there were any query with regard to the request made it was sorted then and then and it was pretty straight forward. But the good all days of face to face communication changed when we started to get requests via email, although a good idea, in principle, requests via email are not without pitfalls and therefore face to face communication between lab staff and pathologist is still necessary. say email request can be problematic as very often request. On Wed, Apr 14, 2010 at 4:09 PM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. What are you doing to minimize the > communication breakdown, when the pathologist are not housed in the lab. > We will be moving to a new lab and the pathologist will not be in the > new bldg with us. They will be in the old building next door. Are you > relying upon email and phone interactions. My directors concern is how > will this effect the flow of information, especially when they are used > to face to face interactions. > > Allison Scott HT(ASCP) > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Maria.Katleba <@t> stjoe.org Wed Apr 14 17:13:52 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Apr 14 17:14:28 2010 Subject: [Histonet] lab week theme Message-ID: Can anyone tell me what this year's Lab Week theme is? Just wondering so that I can work the lab decorations.... Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From lpwenk <@t> sbcglobal.net Wed Apr 14 21:27:54 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Apr 14 21:28:05 2010 Subject: [Histonet] HTL exam In-Reply-To: <4BC5E005.72AC.0059.1@shands.ufl.edu> Message-ID: <87D36A6401A84139981271B9CF284933@HPPav2> That's really hard to say. There are no categories for Enzymes, EM or Cytology. The HT and HTL exams have 5 categories, 100 questions total. The number of questions that a candidate is asked in each category is: - Fixation = 10-25 - Processing/Embedding = 10-14 - Microtomy = 10-14 - Staining = 40-50 - Lab Operations (safety, math, equipment, regulations, etc.) = 10-15 Now, let's talk cytology questions. Was the question about: - what solution the cells were placed in (alcohol, saccomano, etc) = fixative question - the Pap stain, or the Diff Quik = staining question - bloodborne pathogens (BBP), centrifuge, staining GYN and non-GYN separately = lab op questions Same with Enzymes or IHC or EM: - fresh tissue or fixed, and in what = fixation question - time in processing or frozen, paraffin or resin = processing questions - how thick to cut section, or frozen sectioning = microtomy questions - how to do the stains, tissue ID = staining questions - dilutions (math), molar solutions (math), BBP (safety), chemical disposal (safety and regulations), how long to fix breast for ER/PR (regulations), cryostat/microtome/processor (instrument), etc = lab op questions Since there are 1000+ questions in the pool, and since candidates only receive 100, random but in the above percentages, one person might get no questions on, say, cytology, but another person could get 6, someone else 2. Luck of the draw, so to speak. So the person who says "I got 6 cytology questions" - really didn't. They got, say, 2 fixation questions, 3 staining questions, and 1 regulation question. The questions just happened to be on cytology. They could have just as easily been on Gomori Trichrome. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Wednesday, April 14, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what the percentage of enzyme histochemistry, electron microscopy, and cytology questions are for the HTL? Thanks, Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Apr 14 21:31:50 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Apr 14 21:31:58 2010 Subject: [Histonet] lab week theme In-Reply-To: Message-ID: <991BE55D744E4454B937262396F02C4E@HPPav2> Go to www.ascp.org Across the top, click on Laboratory Professionals In the box, Click on Lab Professionals Week. Can find theme, logo, ideas, fast facts, etc. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Wednesday, April 14, 2010 6:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab week theme Can anyone tell me what this year's Lab Week theme is? Just wondering so that I can work the lab decorations.... Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deliadfam <@t> yahoo.com Wed Apr 14 22:14:30 2010 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Wed Apr 14 22:14:34 2010 Subject: [Histonet] Need CLIA help Message-ID: <585182.79580.qm@web63102.mail.re1.yahoo.com> Hello Histonetters, ? There are two issues that have been boggling me that I really cant find a definite answer for. I am hoping to learn from your experiences and what works. Anything helps. ? I work in a CLIA regulated Derm Lab. I would like to ask what is CLIA's requirement at the grossing station as far as QA/QC. And what works for you? Currently the histotechs( OJT or?AAS)?rotate grossing in specimens.?Tech 1?will log in all patient data, assign accession # to the requistion while Tech 2?double-checks and verifies specimen containers to requistions and labels the containers. Then cassettes are made Tech 1 grosses in. We are not documenting anything I am trying to implement a standard but would like to know if its neccesary and what you all are doing? ? Secondly, as far as IHC is concerned what is your standard protocol for validation? And are you all running a negative control for every patient sample as well? I've worked at a CAP reference lab, Research Lab, and Hospital lab?that did not do IHC in-house. CLIA is just unclear to me. Any feedback, advice, or a pointing in the right direction can help me tremendously. ? Thank you all I hope to hear from you soon. :-) ? D. Garcia From disbrc <@t> shands.ufl.edu Wed Apr 14 23:02:25 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Wed Apr 14 23:02:31 2010 Subject: [Histonet] HTL exam In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5DF82BD@LRGHEXVS1.practice.lrgh.org> References: <4BC5E005.72AC.0059.1@shands.ufl.edu> <38667E7FB77ECD4E91BFAEB8D986386323D5DF82BD@LRGHEXVS1.practice.lrgh.org> Message-ID: <4BC65792.72AC.0059.1@shands.ufl.edu> Hi, Come to think of it, there is a study guide and area of interest for most ASCP exams. Thanks! Carrie >>> "Podawiltz, Thomas" 4/14/2010 3:53 PM >>> I believe you can down load that from the ASCP site. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Wednesday, April 14, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what the percentage of enzyme histochemistry, electron microscopy, and cytology questions are for the HTL? Thanks, Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From disbrc <@t> shands.ufl.edu Wed Apr 14 23:05:35 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Wed Apr 14 23:05:41 2010 Subject: [Histonet] HTL exam In-Reply-To: <87D36A6401A84139981271B9CF284933@HPPav2> References: <4BC5E005.72AC.0059.1@shands.ufl.edu> <87D36A6401A84139981271B9CF284933@HPPav2> Message-ID: <4BC65850.72AC.0059.1@shands.ufl.edu> Hi Peggy, Wow, your response is why I love this board. I'm glad I have three months to review the areas not in my daily routine work. Wish me luck, Carrie >>> "Lee & Peggy Wenk" 4/14/2010 10:27 PM >>> That's really hard to say. There are no categories for Enzymes, EM or Cytology. The HT and HTL exams have 5 categories, 100 questions total. The number of questions that a candidate is asked in each category is: - Fixation = 10-25 - Processing/Embedding = 10-14 - Microtomy = 10-14 - Staining = 40-50 - Lab Operations (safety, math, equipment, regulations, etc.) = 10-15 Now, let's talk cytology questions. Was the question about: - what solution the cells were placed in (alcohol, saccomano, etc) = fixative question - the Pap stain, or the Diff Quik = staining question - bloodborne pathogens (BBP), centrifuge, staining GYN and non-GYN separately = lab op questions Same with Enzymes or IHC or EM: - fresh tissue or fixed, and in what = fixation question - time in processing or frozen, paraffin or resin = processing questions - how thick to cut section, or frozen sectioning = microtomy questions - how to do the stains, tissue ID = staining questions - dilutions (math), molar solutions (math), BBP (safety), chemical disposal (safety and regulations), how long to fix breast for ER/PR (regulations), cryostat/microtome/processor (instrument), etc = lab op questions Since there are 1000+ questions in the pool, and since candidates only receive 100, random but in the above percentages, one person might get no questions on, say, cytology, but another person could get 6, someone else 2. Luck of the draw, so to speak. So the person who says "I got 6 cytology questions" - really didn't. They got, say, 2 fixation questions, 3 staining questions, and 1 regulation question. The questions just happened to be on cytology. They could have just as easily been on Gomori Trichrome. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Wednesday, April 14, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what the percentage of enzyme histochemistry, electron microscopy, and cytology questions are for the HTL? Thanks, Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.thotakura <@t> imperial.ac.uk Thu Apr 15 06:14:26 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Thu Apr 15 06:14:32 2010 Subject: [Histonet] IHC and IF Message-ID: Dear All, I have kind of funny problem. I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct labeling of my monoclonal antibody with flurochrome). I am working on mouse liver frozen sections. When I did IHC the staining looks like cytoplasm and if I do IF for the same protein I saw nuclear staining. I am unable to conclude whether it is cytoplasm or nuclei staining. Please advice. The monoclonal antibody I used is not commercially available. We made it. Thanks In advance. Many Thanks, Anil Kumar. From kmerriam2003 <@t> yahoo.com Thu Apr 15 06:38:31 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Apr 15 06:38:37 2010 Subject: [Histonet] IHC and IF In-Reply-To: References: Message-ID: <483498.40375.qm@web50308.mail.re2.yahoo.com> Hi Anil, Perhaps you are having mouse-on-mouse issues with your IHC (DAB) and you are seeing?antibody cross-reactivity with mouse IgGs (you don't mention your IHC detection method, so I could be wrong).? You are probably not seeing this cross-reactivity?with the IF method, because?the labeling is direct.??If that is the case, I would be more likely to trust?what?I saw?with the directly-labeled antibody. If you have labeled the monoclonal antibody with FITC and you want to see it with a chromagen, you could use an anti-FITC secondary (Invitrogen makes a nice Rabbit anti-FITC) which you could then detect?with an anti-Rabbit?polymer?(or whatever you normally?use to detect a rabbit primary). Good luck, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Thotakura, Anil Kumar" To: "Histonet@lists.utsouthwestern.edu" Sent: Thu, April 15, 2010 7:14:26 AM Subject: [Histonet] IHC and IF Dear All, I have kind of funny problem. I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct labeling of my monoclonal antibody with flurochrome). I am working on mouse liver frozen sections. When I did IHC the staining looks like cytoplasm and if I do IF for the same protein I saw nuclear staining. I am unable to conclude whether it is cytoplasm or nuclei staining. Please advice. The monoclonal antibody I used is not commercially available. We made it. Thanks In advance. Many Thanks, Anil Kumar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Thu Apr 15 06:43:58 2010 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Thu Apr 15 07:15:03 2010 Subject: [Histonet] IHC and IF Message-ID: hi if u use triton for permeablization in IHC it sometimes cause the stain pattern to diffuse from nucleus to cytoplasm it is common ------------------ Original ------------------ From: "Kim Merriam"; Date: Thu, Apr 15, 2010 07:38 PM To: "Thotakura, Anil Kumar"; "Histonet"; Subject: Re: [Histonet] IHC and IF Hi Anil, Perhaps you are having mouse-on-mouse issues with your IHC (DAB) and you are seeing antibody cross-reactivity with mouse IgGs (you don't mention your IHC detection method, so I could be wrong). You are probably not seeing this cross-reactivity with the IF method, because the labeling is direct. If that is the case, I would be more likely to trust what I saw with the directly-labeled antibody. If you have labeled the monoclonal antibody with FITC and you want to see it with a chromagen, you could use an anti-FITC secondary (Invitrogen makes a nice Rabbit anti-FITC) which you could then detect with an anti-Rabbit polymer (or whatever you normally use to detect a rabbit primary). Good luck, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Thotakura, Anil Kumar" To: "Histonet@lists.utsouthwestern.edu" Sent: Thu, April 15, 2010 7:14:26 AM Subject: [Histonet] IHC and IF Dear All, I have kind of funny problem. I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct labeling of my monoclonal antibody with flurochrome). I am working on mouse liver frozen sections. When I did IHC the staining looks like cytoplasm and if I do IF for the same protein I saw nuclear staining. I am unable to conclude whether it is cytoplasm or nuclei staining. Please advice. The monoclonal antibody I used is not commercially available. We made it. Thanks In advance. Many Thanks, Anil Kumar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Apr 15 07:22:26 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Apr 15 07:23:13 2010 Subject: SPAM-LOW: Re: [Histonet] IHC and IF In-Reply-To: <483498.40375.qm@web50308.mail.re2.yahoo.com> References: <483498.40375.qm@web50308.mail.re2.yahoo.com> Message-ID: I agree with Kim, your cytoplasmic staining with hrp/dab is probably because your anti mouse detection is reacting to endogenous Mouse IgG in the mouse tissue you are using. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, April 15, 2010 5:39 AM To: Thotakura, Anil Kumar; Histonet Subject: SPAM-LOW: Re: [Histonet] IHC and IF Hi Anil, Perhaps you are having mouse-on-mouse issues with your IHC (DAB) and you are seeing?antibody cross-reactivity with mouse IgGs (you don't mention your IHC detection method, so I could be wrong).? You are probably not seeing this cross-reactivity?with the IF method, because?the labeling is direct.??If that is the case, I would be more likely to trust?what?I saw?with the directly-labeled antibody. If you have labeled the monoclonal antibody with FITC and you want to see it with a chromagen, you could use an anti-FITC secondary (Invitrogen makes a nice Rabbit anti-FITC) which you could then detect?with an anti-Rabbit?polymer?(or whatever you normally?use to detect a rabbit primary). Good luck, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Thotakura, Anil Kumar" To: "Histonet@lists.utsouthwestern.edu" Sent: Thu, April 15, 2010 7:14:26 AM Subject: [Histonet] IHC and IF Dear All, I have kind of funny problem. I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct labeling of my monoclonal antibody with flurochrome). I am working on mouse liver frozen sections. When I did IHC the staining looks like cytoplasm and if I do IF for the same protein I saw nuclear staining. I am unable to conclude whether it is cytoplasm or nuclei staining. Please advice. The monoclonal antibody I used is not commercially available. We made it. Thanks In advance. Many Thanks, Anil Kumar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Apr 15 09:54:05 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Apr 15 09:55:19 2010 Subject: [Histonet] Re: IHC mouse antibody on mouse tissue Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BAE97BF@KCL-MAIL04.kclad.ds.kcl.ac.uk> Imho....just do it on your tissue and include a no primary control. If the latter is clear, don't worry about MOM. If it is not, then we can advise further. You don't state what Ab/tissue/detection system you are using...... This would help a lot as protocols will vary according to the tissue used . I never want to use a more complicated detection system than I have to, just because it is MOM. Occam's razor! carl From amrithraj_nair <@t> merck.com Thu Apr 15 11:04:54 2010 From: amrithraj_nair <@t> merck.com (Nair, Amrithraj M) Date: Thu Apr 15 11:05:06 2010 Subject: [Histonet] Cytokeratin-7 IHC on FFPE Message-ID: Do anyone have experience with IHC staining for CK-7 in mouse pancreas? If so would you mind sharing that info. I have had limited success with this Amrith Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Bill.Tench <@t> pph.org Thu Apr 15 12:08:58 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Apr 15 12:09:08 2010 Subject: [Histonet] CLIA requirements Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02862F97@MAIL1.pph.local> You may define whatever QA monitors for grossing that you wish. These are not CLIA mandated, but contribute to improved performance. When it comes to the individuals doing the grossing, you need to understand that this has been interpreted as a high complexity activity with strict CLIA requirements (see recent postings and CAP standards). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From igor.deyneko <@t> gmail.com Thu Apr 15 12:10:26 2010 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Thu Apr 15 12:10:30 2010 Subject: [Histonet] Murine and Human prenatal Fetuses Message-ID: Dear Histonetters! I am looking for commercially available paraffin sections of the prenatal murine & human fetuses. I would appreciate any information. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From akemiat3377 <@t> yahoo.com Thu Apr 15 12:33:50 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Apr 15 12:34:26 2010 Subject: [Histonet] Histotech wages in AZ Message-ID: <3E8E8EE3-2705-47DE-A0F7-D2057B37D9E8@yahoo.com> Hello all of you histologists in AZ, I have been requested to gather data regarding current wage scales for HT's and HTL's in AZ from a local facility. In recent years, larger laboratories such as Mayo Clinic and now, MD Anderson have expanded their services to the Phoenix metropolitan area. These labs have offered higher hourly rates than currently paid to local histologists. The facility I am working with is relatively small, but they would like to be competitive. I would also like to know what benefits your facilities provide. Is your facility paying for the total expense for health coverage, 401 K, vision, dental coverage etc....... Thank you in advance for your information. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemi3377@gmail.com From victor <@t> pathology.washington.edu Thu Apr 15 13:19:20 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Apr 15 13:20:02 2010 Subject: [Histonet] Fwd: [PPMA] Barcoding in Boston! Message-ID: <4BC758A8.6020205@pathology.washington.edu> For any of you in the Boston area, my boss will be speaking on barcoding. Victor -------- Original Message -------- Subject: [PPMA] Barcoding in Boston! Date: Thu, 15 Apr 2010 10:34:31 -0700 (Pacific Daylight Time) From: Rodney Schmidt Reply-To: A mutual assistance forum for PowerPath users To: PowerPath Mutual Assistance The topic of barcoding in pathology has suddenly gotten extremely popular. Just about all the hardware and LIS exhibitors at the recent USCAP meeting featured their capabilities. Not all solutions are equal but it takes a knowledgeable user to tell the difference between the solutions that are good for users vs the ones that are good for the vendors. Most of you know that the University of Washington has built out a complete, sophisticated, and highly user-friendly barcoding and workflow-driving system that handles all pathology materials (OmniTrax). It's being used at multiple academic and private practices (PowerPath sites) and has recently been revised so that it can work with any LIS. Our experience with OmniTrax has let us understand deeply exactly what makes a barcoding system successful from a user's perspective, including likely benefits to labs in terms of cost savings and error reduction. In the last year, I've been invited to give talks about this at APIII, USCAP, NSH, and AAPA meetings, as well as at other sites. Thermo Scientific has asked me to speak on this topic next Wednesday in Boston. If you're interested and in the area, please contact your Thermo rep for information. Rod Rodney A. Schmidt, M.D., Ph.D. Professor of Pathology Director of Medical Informatics University of Washington, Seattle, WA (206) 598-6462 (206) 344-0532 (pager) (206) 598-3803 (fax) ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ PowerPath Mutual Assistance [PPMA] mailing list PPMA@u.washington.edu http://mailman2.u.washington.edu/mailman/listinfo/ppma From mdavis2 <@t> lancastergeneral.org Thu Apr 15 13:32:44 2010 From: mdavis2 <@t> lancastergeneral.org (Davis, Michael J) Date: Thu Apr 15 13:32:50 2010 Subject: [Histonet] Leica Peloris Message-ID: <166C91FDD3F266448D8528CBD0E684D20141FEABDD7D@MAIL-AG-CLUSTER.lha.org> Moving into a new lab and have tested several processors. Need some info on the peloris. Good and Bad. We demoed the machine for about 2 months and had an issue with our biopsy tissue staining too light (H&E). We could not get this problem resolved - we were running our tissue thru the xylene free process. Also any truth to the rumor that I heard today thay the FDA has placed a hold on this machine? Michael J Davis, HT (ASCP) Histology Lancaster General Hospital 555 N Duke St Lancaster, PA 17601 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Barry.R.Rittman <@t> uth.tmc.edu Thu Apr 15 13:50:26 2010 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Apr 15 13:50:31 2010 Subject: [Histonet] Request TRAP protocol Message-ID: <75A0543E23D3A7458012D9E02EDBEC00098E734B61@UTHCMS1.uthouston.edu> Can someone please let me know the best technique(s) they currently use for demonstrating TRAP and alkaline phosphatase for osteoblasts and osteoclasts in formalin fixed paraffin sections or resin sections? Thanks Barry From mpence <@t> grhs.net Thu Apr 15 13:57:38 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Apr 15 13:57:42 2010 Subject: [Histonet] Leica Peloris In-Reply-To: <166C91FDD3F266448D8528CBD0E684D20141FEABDD7D@MAIL-AG-CLUSTER.lha.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DC8@is-e2k3.grhs.net> We use the Peloris and have had no big problems with it. Certainly not that affect our stain, however, we do not use it xylene-free. Her may lay the problem. I think the FDA question should be answered by someone from Leica. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Michael J Sent: Thursday, April 15, 2010 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica Peloris Moving into a new lab and have tested several processors. Need some info on the peloris. Good and Bad. We demoed the machine for about 2 months and had an issue with our biopsy tissue staining too light (H&E). We could not get this problem resolved - we were running our tissue thru the xylene free process. Also any truth to the rumor that I heard today thay the FDA has placed a hold on this machine? Michael J Davis, HT (ASCP) Histology Lancaster General Hospital 555 N Duke St Lancaster, PA 17601 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Thu Apr 15 14:01:53 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Apr 15 14:02:00 2010 Subject: [Histonet] Leica Peloris In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DC8@is-e2k3.grhs.net> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6232@EXCHANGECLUSTER.yumaregional.local> Please answer this we are looking at buying one next year! Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, April 15, 2010 11:58 AM To: Davis, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Peloris We use the Peloris and have had no big problems with it. Certainly not that affect our stain, however, we do not use it xylene-free. Her may lay the problem. I think the FDA question should be answered by someone from Leica. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Michael J Sent: Thursday, April 15, 2010 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica Peloris Moving into a new lab and have tested several processors. Need some info on the peloris. Good and Bad. We demoed the machine for about 2 months and had an issue with our biopsy tissue staining too light (H&E). We could not get this problem resolved - we were running our tissue thru the xylene free process. Also any truth to the rumor that I heard today thay the FDA has placed a hold on this machine? Michael J Davis, HT (ASCP) Histology Lancaster General Hospital 555 N Duke St Lancaster, PA 17601 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From leiker <@t> buffalo.edu Thu Apr 15 14:08:05 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Apr 15 14:08:15 2010 Subject: [Histonet] ms References: <7C6EDC47B2AE4F168AC905D06FFA3A52@Patsyoffice> Message-ID: Hi Patsy, I'd like to verify the retrieval temp? In a previous email you wrote "70dc" and here you wrote "80dc." Thank you. Regards, Merced --On Wednesday, April 14, 2010 10:54 AM -0600 Patsy Ruegg wrote: > For all those looking for this info from me. > > > > I use the serotech mouse anti porcine CD31 on pig tonsil at 1/10-1/50 > overnight after HIER in a waterbath set at 80dc for 3 hours with prewarmed > citrate buffer ph6. I use this with an anti mouse labeled polymer > detection system, either hrp/dab or alk.phos./red. You can join the > group online at www.ihcrg.org > > > > This one is tuff, but this is what worked for us. > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) ('the intended recipient') to whom it was addressed. Any views > or opinions presented are solely those of the author. It may contain > information that is privileged & confidential within the meaning of > applicable law. Accordingly any dissemination, distribution, copying, or > other use of this message, or any of its contents, by any person other > than the intended recipient may constitute a breach of civil or criminal > law and is strictly prohibited. If you are NOT the intended recipient > please contact the sender and dispose of this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From alisha <@t> ka-recruiting.com Thu Apr 15 14:43:39 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Apr 15 14:43:35 2010 Subject: [Histonet] Great Histotech Jobs! Message-ID: <1429523602.1271360619441.JavaMail.cfservice@sl4app3> Hi Histotechs, I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histotechs into permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? Atlanta, GA - One particular client that I am working with is a financially sound, medically advanced, full-service hospital in Atlanta, GA. My client is looking for both a Histotechnologist certified histotechnologist and a Pathology Coordinator for their hospital. Both positions would work a day shift. The Histotechnologist position requires an HTL(ASCP) certification and at least 5 years experience. The Pathology Coordinator position requires either a HT, HTL, or CT (ASCP) certification, alongwith 5+ years in histology/cytology, and 4+ years in a supervisory role. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: Histotechs/Cytotechs NY - New York City - Histotech 3rd shift NV -Las Vegas - Histotech 3rd shift NV Las Vegas - Histology Supervisor - 3rd shift PA - Cytology Supervisor CA - Southern - Histotech NH - HTL (dayshift) NH - HT (day shift) GA - Atlanta - Histotech GA - Atlanta - Pathology Coordinator (HT or CT) MA - IHC Tech MA - Histotech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus. Read Below to Find Out More: Do you know of anyone who is a great histotech and may be interested in hearing about other opportunities? Would you like to make $500 if you refer a histotech to us that we place into a new job? If so, please send us names and contact information of any histotech you know...if now or in the future we place that histotech into a new job, you will receive a $500 referral bonus. If you would like to keep the referral confidential - just write that in your notes. To refer a histotech: Go to: http://www.ka-recruiting.com/ Scroll to the bottom of the page to enter in your referrals. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From jtaylor <@t> meriter.com Thu Apr 15 14:56:47 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Apr 15 14:56:52 2010 Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Message-ID: <466B666475DE6547BBB0641E540A4BB5068763539F@EXVS1.meriter.com> Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From mucram11 <@t> comcast.net Thu Apr 15 14:59:31 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Apr 15 14:59:33 2010 Subject: [Histonet] DAKO Autostainer Link 48/FLEX system In-Reply-To: <466B666475DE6547BBB0641E540A4BB5068763539F@EXVS1.meriter.com> Message-ID: <1970959645.31111271361570976.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I am in need of the same information if possible. Pam Marcum UAMS Little Rock AR ----- Original Message ----- From: "Jean Taylor" To: "ihcrg@googlegroups.com" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, April 15, 2010 2:56:47 PM GMT -06:00 US/Canada Central Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Apr 15 15:12:00 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Apr 15 15:13:01 2010 Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system In-Reply-To: <466B666475DE6547BBB0641E540A4BB5068763539F@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB5068763539F@EXVS1.meriter.com> Message-ID: I have upgraded to the new autostainer links. I have four of them. There are many pros and many cons. If you have any specific questions I'd be happy to answer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor@meriter.com] Sent: Thursday, April 15, 2010 3:56 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Apr 15 15:14:56 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Apr 15 15:15:47 2010 Subject: [Histonet] DAKO Autostainer Link 48/FLEX system In-Reply-To: <1970959645.31111271361570976.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <466B666475DE6547BBB0641E540A4BB5068763539F@EXVS1.meriter.com>, <1970959645.31111271361570976.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: I have upgraded to the new autostainer links. I have four of them. There are many pros and many cons. Too many to list. If you have any specific questions I'd be happy to answer them. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum [mucram11@comcast.net] Sent: Thursday, April 15, 2010 3:59 PM To: Jean Taylor Cc: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: Re: [Histonet] DAKO Autostainer Link 48/FLEX system I am in need of the same information if possible. Pam Marcum UAMS Little Rock AR ----- Original Message ----- From: "Jean Taylor" To: "ihcrg@googlegroups.com" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, April 15, 2010 2:56:47 PM GMT -06:00 US/Canada Central Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aallison <@t> wpalabs.com Thu Apr 15 17:24:18 2010 From: aallison <@t> wpalabs.com (aallison@wpalabs.com) Date: Thu Apr 15 17:24:22 2010 Subject: [Histonet] Part time position in Phoenix Message-ID: <20100415152418.85fdaa4bc40941c5dd00a74b80284d9b.9763dd0efc.wbe@email04.secureserver.net> Western Pathology Associates, Ltd, a pathology reference laborato has an opening for a Histotechnologist (Part-time Monday through Friday located in a Phoenix, AZ. Primary Responsibilities: The successful candidate must be able to work high degree of technical proficiency. Primary respons be to embed, cut and stain H&E?s on biopsy speci Required Skills/Experience: Requires HT (ASCP) certificati experience working with small biopsies. Wes is committed to providing quality patient care. S determined based on the overall skills and background of the applicant. To Apply Please send Resume by Email or Please no phone calls about this position from candidates or recrui ters. Akemi Allison BS, HT (ASCP) HTL Manager, Anatomical Patholo Western Pathology Associates Fax: 602.861.3500 E-Mail: aallison From brimlee16 <@t> gmail.com Fri Apr 16 04:18:54 2010 From: brimlee16 <@t> gmail.com (Sandra Peltier) Date: Fri Apr 16 04:18:58 2010 Subject: [Histonet] Leica Peloris In-Reply-To: <166C91FDD3F266448D8528CBD0E684D20141FEABDD7D@MAIL-AG-CLUSTER.lha.org> References: <166C91FDD3F266448D8528CBD0E684D20141FEABDD7D@MAIL-AG-CLUSTER.lha.org> Message-ID: I LOVE my Peloris. Both of them equally!! On Thu, Apr 15, 2010 at 2:32 PM, Davis, Michael J wrote: > Moving into a new lab and have tested several processors. ?Need some info on the peloris. ?Good and Bad. ?We demoed the machine for about 2 months and had an issue with our biopsy tissue staining too light (H&E). ?We could not get this problem resolved - we were running our tissue thru the xylene free process. ?Also any truth to the rumor that I heard today thay the FDA has placed a hold on this machine? > > Michael J Davis, HT (ASCP) > Histology > Lancaster General Hospital > 555 N Duke St > Lancaster, PA 17601 > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. ?Any unauthorized > review, use, disclosure or distribution is prohibited. ?If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From billodonnell <@t> catholichealth.net Fri Apr 16 07:51:16 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Apr 16 07:51:28 2010 Subject: [Histonet] Dako rep In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4703D@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4703D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Would the Dako rep for Kearney, NE please contact me? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 bill@deaconbill.com From cjbulmer <@t> sbcglobal.net Fri Apr 16 08:40:14 2010 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Fri Apr 16 08:40:20 2010 Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system In-Reply-To: Message-ID: <954307.19408.qm@web82308.mail.mud.yahoo.com> Hey Loralee, ? Could you post a few of the pros and cons?? Do you like the new system? Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX --- On Thu, 4/15/10, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system To: "Taylor, Jean" , "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Date: Thursday, April 15, 2010, 8:12 PM I have upgraded to the new autostainer links.? I have four of them. There are many pros and many cons.? If you have any specific questions I'd be happy to answer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor@meriter.com] Sent: Thursday, April 15, 2010 3:56 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Apr 16 10:27:27 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Apr 16 10:27:33 2010 Subject: [Histonet] Leica Peloris In-Reply-To: Message-ID: <136272.21828.qm@web54207.mail.re2.yahoo.com> We love ours....we've had no problems... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Fri, 4/16/10, Sandra Peltier wrote: From: Sandra Peltier Subject: Re: [Histonet] Leica Peloris To: "Davis, Michael J" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, April 16, 2010, 2:18 AM I LOVE my Peloris.? Both of them equally!! On Thu, Apr 15, 2010 at 2:32 PM, Davis, Michael J wrote: > Moving into a new lab and have tested several processors. ?Need some info on the peloris. ?Good and Bad. ?We demoed the machine for about 2 months and had an issue with our biopsy tissue staining too light (H&E). ?We could not get this problem resolved - we were running our tissue thru the xylene free process. ?Also any truth to the rumor that I heard today thay the FDA has placed a hold on this machine? > > Michael J Davis, HT (ASCP) > Histology > Lancaster General Hospital > 555 N Duke St > Lancaster, PA 17601 > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. ?Any unauthorized > review, use, disclosure or distribution is prohibited. ?If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Apr 16 10:40:48 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Apr 16 10:41:00 2010 Subject: [Histonet] Leica Peloris In-Reply-To: <136272.21828.qm@web54207.mail.re2.yahoo.com> References: <136272.21828.qm@web54207.mail.re2.yahoo.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0DB@EXCHMBC2.ad.ah.local> We love our Peloris. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, April 16, 2010 10:27 AM To: Sandra Peltier Cc: Histonet Subject: Re: [Histonet] Leica Peloris We love ours....we've had no problems... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! --- On Fri, 4/16/10, Sandra Peltier wrote: From: Sandra Peltier Subject: Re: [Histonet] Leica Peloris To: "Davis, Michael J" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, April 16, 2010, 2:18 AM I LOVE my Peloris. Both of them equally!! On Thu, Apr 15, 2010 at 2:32 PM, Davis, Michael J wrote: > Moving into a new lab and have tested several processors. Need some info on the peloris. Good and Bad. We demoed the machine for about 2 months and had an issue with our biopsy tissue staining too light (H&E). We could not get this problem resolved - we were running our tissue thru the xylene free process. Also any truth to the rumor that I heard today thay the FDA has placed a hold on this machine? > > Michael J Davis, HT (ASCP) > Histology > Lancaster General Hospital > 555 N Duke St > Lancaster, PA 17601 > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From akemiat3377 <@t> yahoo.com Fri Apr 16 10:47:29 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Apr 16 10:48:25 2010 Subject: [Histonet] Sorry... Part time position in Phoenix In-Reply-To: <20100415152418.85fdaa4bc40941c5dd00a74b80284d9b.9763dd0efc.wbe@email04.secureserver.net> References: <20100415152418.85fdaa4bc40941c5dd00a74b80284d9b.9763dd0efc.wbe@email04.secureserver.net> Message-ID: <1911CBD0-6B9E-454B-AF1D-B2085E855EC5@yahoo.com> Hi All, Sorry, I created a Job posting and copied it to email the subscribers on histonet. The text went whacky! I will submit it again. Guess copying doesn't work very well. Akemi Allison On Apr 15, 2010, at 3:24 PM, aallison@wpalabs.com wrote: > > Western Pathology Associates, Ltd, a pathology reference > laborato ry > has an opening for a Histotechnologist (Part-time Monday > through > Friday from 11:00 PM to 3:00 AM). We are a state-of-the-art > facility > located in a brand new lab next to John C. Lincoln Hospital in > North > Phoenix, AZ. > Primary Responsibilities: > The successful candidate must be able to work independently > with a > high degree of technical proficiency. Primary respons ibilities > will > be to embed, cut and stain H&E?s on biopsy speci mens. > Required Skills/Experience: > Requires HT (ASCP) certificati on with a minimum of 4 > years of > experience working with small biopsies. Wes tern Pathology > Associates > is committed to providing quality patient care. S alary > will be > determined based on the overall skills and background of the > chosen > applicant. > To Apply Please send Resume by Email or Fax. > Please no phone calls about this position from candidates or > recrui ters. > Akemi Allison BS, HT (ASCP) HTL > Manager, Anatomical Patholo gy > Western Pathology Associates > Fax: 602.861.3500 > E-Mail: aallison @wpalabs.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo007 <@t> hotmail.com Fri Apr 16 11:04:21 2010 From: histo007 <@t> hotmail.com (James Ball) Date: Fri Apr 16 11:04:26 2010 Subject: [Histonet] (no subject) Message-ID: I was hoping that some one in the vet sector might have an idea of a course of treatment for a sheapard bread that is experiencing ulcerations on his tail. I realise that with limited information shuch as ulceration is very little to go on, I was hoping it might be a common condition sonething like basal cell Ca in in humans I am not trying to circumvent the vet I have invested well over 500 dollars with the vet with nothing to show for my efforts except for a dog that is in pain and will not leace the area alone to heal. The problem has been going on for about two weeks. _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 From foreightl <@t> gmail.com Fri Apr 16 11:51:39 2010 From: foreightl <@t> gmail.com (Pat Laurie) Date: Fri Apr 16 11:51:44 2010 Subject: [Histonet] Leica Peloris In-Reply-To: <166C91FDD3F266448D8528CBD0E684D20141FEABDD7D@MAIL-AG-CLUSTER.lha.org> References: <166C91FDD3F266448D8528CBD0E684D20141FEABDD7D@MAIL-AG-CLUSTER.lha.org> Message-ID: We love all three of our pelori (sp?) We plan on buying another one in the next year. On Thu, Apr 15, 2010 at 11:32 AM, Davis, Michael J < mdavis2@lancastergeneral.org> wrote: > Moving into a new lab and have tested several processors. Need some info > on the peloris. Good and Bad. We demoed the machine for about 2 months and > had an issue with our biopsy tissue staining too light (H&E). We could not > get this problem resolved - we were running our tissue thru the xylene free > process. Also any truth to the rumor that I heard today thay the FDA has > placed a hold on this machine? > > Michael J Davis, HT (ASCP) > Histology > Lancaster General Hospital > 555 N Duke St > Lancaster, PA 17601 > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From annigyg <@t> gmail.com Fri Apr 16 12:36:17 2010 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Apr 16 12:36:22 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: take the poor animal to a vet - if it is a BCC or an SCC (which are both CANCER by the way..and it can spread) then it needs proper treatment would you treat your child like this - I hope not and just by the way....this is not a pharmacy listserver On 16 April 2010 20:04, James Ball wrote: > > > I was hoping that some one in the vet sector > might have an idea of a course of treatment for a sheapard bread that is > experiencing ulcerations on his tail. I realise that with limited > information > shuch as ulceration is very little to go on, I was hoping it might be a > common > condition sonething like basal cell Ca in in humans > I am not trying to circumvent the vet I have > invested well over 500 dollars with the vet with nothing to show for my > efforts > except for a dog that is in pain and will not leace the area alone to heal. > The > problem has been going on for about two weeks. > _________________________________________________________________ > The New Busy is not the old busy. Search, chat and e-mail from your inbox. > > http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Janice.Mahoney <@t> alegent.org Fri Apr 16 12:44:17 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Apr 16 12:44:30 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A0E0@EXCHMBC2.ad.ah.local> Happy Friday Everyone! Jan Omaha Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Kristopher.Kalleberg <@t> unilever.com Fri Apr 16 12:44:44 2010 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Fri Apr 16 12:44:49 2010 Subject: [Histonet] hematoxylin-eosin saffron Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0819ACAA@NTRSEVS30002.s3.ms.unilever.com> Can someone please explain to me the purpose of a hematoxylin-eosin saffron stain. Why would this be used over the normal hematoxylin and eaosin stain. Thank you in advance. Kris From bhewlett <@t> cogeco.ca Fri Apr 16 13:16:51 2010 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Apr 16 13:16:53 2010 Subject: [Histonet] hematoxylin-eosin saffron References: <0E6BC087F70F9C47ACFF2C203D6E329C0819ACAA@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <63EBC2E0796442C998A1AE4E795C19B7@mainbox> Kris, It is a general oversight stain as is the H&E. It is correctly refered to as the Masson Hematoxylin-Phloxine-Saffron (HPS) stain. Although some people substitute eosin for phloxine, this is a generally inferior technique to HPS (I have NEVER seen a good one!). Technically, the method is harder to control than the H&E or a separate Trichrome, and Saffron is much more expensive than other suitable yellow substitutes. However, those labs that still use the method like it because it has many of the attributes of a trichrome stain. Bryan ----- Original Message ----- From: "Kalleberg, Kristopher" To: Sent: Friday, April 16, 2010 1:44 PM Subject: [Histonet] hematoxylin-eosin saffron Can someone please explain to me the purpose of a hematoxylin-eosin saffron stain. Why would this be used over the normal hematoxylin and eaosin stain. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Fri Apr 16 14:01:48 2010 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Fri Apr 16 14:02:11 2010 Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Message-ID: <4BC85FBC020000A80004262E@ns.luhcares.org> We changed from the older DAKO system to the link 48. There were some growing pains as there is always when changing to new instrumentation. The pros is that once your trained on the software it is alot more user friendly then the older system it is also able to have other protocals programed into it, a very open system. The only cons we have had is a few issuse with the PT link and some warped racks. DAKO has been very responsive with any of the issues and the training they provided was great. I hope this helps. Matt Lunetta HT (ASCP) Longmont United Hospital Message: 11 Date: Thu, 15 Apr 2010 14:56:47 -0500 From: "Taylor, Jean" Subject: [Histonet] DAKO Autostainer Link 48/FLEX system To: "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <466B666475DE6547BBB0641E540A4BB5068763539F@EXVS1.meriter.com> Content-Type: text/plain; charset="us-ascii" Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ------------------------------ Message: 12 Date: Thu, 15 Apr 2010 19:59:31 +0000 (UTC) From: Pamela Marcum Subject: Re: [Histonet] DAKO Autostainer Link 48/FLEX system To: Jean Taylor Cc: "histonet@lists.utsouthwestern.edu" , "ihcrg@googlegroups.com" Message-ID: <1970959645.31111271361570976.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I am in need of the same information if possible. Pam Marcum UAMS Little Rock AR ----- Original Message ----- From: "Jean Taylor" To: "ihcrg@googlegroups.com" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, April 15, 2010 2:56:47 PM GMT -06:00 US/Canada Central Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 15 Apr 2010 16:12:00 -0400 From: "McMahon, Loralee A" Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system To: "Taylor, Jean" , "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I have upgraded to the new autostainer links. I have four of them. There are many pros and many cons. If you have any specific questions I'd be happy to answer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor@meriter.com] Sent: Thursday, April 15, 2010 3:56 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 15 Apr 2010 16:14:56 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] DAKO Autostainer Link 48/FLEX system To: Pamela Marcum , Jean Taylor Cc: "histonet@lists.utsouthwestern.edu" , "ihcrg@googlegroups.com" Message-ID: Content-Type: text/plain; charset="us-ascii" I have upgraded to the new autostainer links. I have four of them. There are many pros and many cons. Too many to list. If you have any specific questions I'd be happy to answer them. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum [mucram11@comcast.net] Sent: Thursday, April 15, 2010 3:59 PM To: Jean Taylor Cc: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: Re: [Histonet] DAKO Autostainer Link 48/FLEX system I am in need of the same information if possible. Pam Marcum UAMS Little Rock AR ----- Original Message ----- From: "Jean Taylor" To: "ihcrg@googlegroups.com" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, April 15, 2010 2:56:47 PM GMT -06:00 US/Canada Central Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Fri Apr 16 14:40:53 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Fri Apr 16 14:41:15 2010 Subject: [Histonet] RE: Diastase (Aspergillium Orzae) In-Reply-To: References: Message-ID: Hey, Does anyone know if Diastase (Aspergillium orzae) can be used in the digestion step of PAS for glycogen? Our vendor sent us four bottles of this as a replacement for Malt Diastase. Just wondering if anyone out there knows something about this product. I searched on the internet, with no luck for apparent laboratory uses. Thanks! Kristie This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Apr 16 14:43:15 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Apr 16 14:43:24 2010 Subject: [Histonet] Phospho antibodies and fixation Message-ID: <7B310892042DA74CB3590053F424CFE60B7480EFBC@ITS-HCWNEM06.ds.Vanderbilt.edu> Good day colleagues, Does anyone have any information on phospho antibodies and fixation? Is there any reason to NOT fix specimens in formalin vs say, 70% EtOH? (and then process them through formalin later?) I can't seem to find any information on whether or not fixation in formalin does something strange to a phosphorylated protien and makes it a less accessible antigen. Also, on that same note, does retreival do anything to it? I am assuming that these antibodies go through the same testing for cross reactivity, etc (depending on the vendor) and are reliable when used properly (like any other antibody). I don't, however, know if there is enough of a difference in the epitope (the fact that it is phosphorylated) that would make it more susceptible to some strange cross linking with formalin (especiallly with phosphate buffered formalin). Any help with this topic would be greatly appreciated as I am uneducated in this area. Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 From macveigh <@t> usc.edu Fri Apr 16 14:50:40 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Apr 16 14:50:38 2010 Subject: [Histonet] Does anyone have and use Shandon Excelsior EX Message-ID: <15EEF32874DF4E6EB5F22368F2284AB1@DFS66DD1> Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle From disbrc <@t> shands.ufl.edu Fri Apr 16 15:17:09 2010 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Fri Apr 16 15:17:17 2010 Subject: [Histonet] dog with tail lacerations Message-ID: <4BC88D82.72AC.0059.1@shands.ufl.edu> Hi, You might want to access IVIS, a compilation of veterinary presentations from meetings and journal articles. It is free. You can then do a search for "lick granulomas", tail injuries, etc. I know of cases where the dog has been sleeping next to the door on the inside and the owner opens the door from the outside catching the tail under the door. This can cause an slow healing injury. There are many possible explanations. Good luck. Carrie From aallison <@t> wpalabs.com Fri Apr 16 15:32:30 2010 From: aallison <@t> wpalabs.com (aallison@wpalabs.com) Date: Fri Apr 16 15:32:35 2010 Subject: [Histonet] Part Time Histology job in Phoenix Message-ID: <20100416133230.85fdaa4bc40941c5dd00a74b80284d9b.59e80e6de4.wbe@email04.secureserver.net> Western Pathology Associates, Ltd, a pathology reference laborato has an opening for a Histotechnologist (Part-time M-F from 10:00 PM to 2 progr We are a new state-of-the-art laboratory located next to John C. Linco committed to p person for the job. The successful candidate must be able to work independently with a hig be t specimens. Requires HT (ASCP) certification with a minimum of 4 years experience overa To apply please contact by email or fax. PLEASE NO PHONE CALLS A BOUT THIS POSITION FROM CANDIDATE OR RECRUITERS. Akemi Allison BS, H Manager, Anatomical Pathology Western Pathology Associates Fax: 602.861.3500 E-Mail: [1]aallison@wpalabs.com References 1. 3D"mailto:aallison@wpalabs.com" From pruegg <@t> ihctech.net Sat Apr 17 10:57:31 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 17 10:58:16 2010 Subject: [Histonet] Phospho antibodies and fixation In-Reply-To: <7B310892042DA74CB3590053F424CFE60B7480EFBC@ITS-HCWNEM06.ds.Vanderbilt.edu> References: <7B310892042DA74CB3590053F424CFE60B7480EFBC@ITS-HCWNEM06.ds.Vanderbilt.edu> Message-ID: Ashley, Contact me directly about phospho antibodies. I have done a lot of studies and used some different fixatives other than formalin, but the issue is complicated. One does not necessarily have control over whither or not you are actually capturing the phosphorylation event or not. There is a company in my building called PhosphoSolutions that sells Phospho antibodies exclusively, the owner is Mike Browning and he, others and I did a workshop at the Denver NSH S/C on this subject. I have some references and protocol for fixatives I can provide you, but the fact of the matter is that it did not seem to make any difference in the results if we used formalin over a special fixative, what did make a difference is time to fixative, that must be very well controlled and you should get the tissues into fixative asap. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Friday, April 16, 2010 1:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Phospho antibodies and fixation Good day colleagues, Does anyone have any information on phospho antibodies and fixation? Is there any reason to NOT fix specimens in formalin vs say, 70% EtOH? (and then process them through formalin later?) I can't seem to find any information on whether or not fixation in formalin does something strange to a phosphorylated protien and makes it a less accessible antigen. Also, on that same note, does retreival do anything to it? I am assuming that these antibodies go through the same testing for cross reactivity, etc (depending on the vendor) and are reliable when used properly (like any other antibody). I don't, however, know if there is enough of a difference in the epitope (the fact that it is phosphorylated) that would make it more susceptible to some strange cross linking with formalin (especiallly with phosphate buffered formalin). Any help with this topic would be greatly appreciated as I am uneducated in this area. Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Apr 17 12:27:21 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Apr 17 12:27:26 2010 Subject: [Histonet] Re: hematoxylin-eosin saffron Message-ID: The hematoxylin-phloxine-saffron (HPS) stain is a trichrome stain that came into use as a "general oversight" stain by surgical pathologists in the 1930's, notably at Columbia-Presbyterian Hospital in New York City. The Goldner-Foot trichrome stain was also used for this purpose, on the other side of Central Park at Cornell Medical Center, probably abandoned after Chandler Foot's retirement in 1948. I actually saw the HPS stain in use at Columbia-Presbyterian around 1966, in place of H & E. I understand that it is still in very limited use in a few laboratories today. Saffron (a natural product, the stigmas of the flowers of Crocus sativus) is extremely expensive - currently $66 for a quarter ounce (about 7 grams) at Penzeys.com. It's used histologically as an alcoholic extract (supposedly best done with a reflux condenser) and can actually be purchased in that form. You can find the HPS staining procedure in older books, such as the AFIP manual. As a working surgical pathologist, I find this ancient technique intriguing but totally impractical in today's world. It's stretching it to get a trichrome stain for your liver biopsies these days. Bob Richmond Samurai Pathologist Knoxville TN From jm.lapointe <@t> accellab.com Sat Apr 17 15:47:48 2010 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Sat Apr 17 15:47:58 2010 Subject: [Histonet] Phospho antibodies and fixation References: <201004171702.o3HH2UT9001574@gateway8.lastspam.com> Message-ID: Hi Ashley, I did a little bit of work on this a few years back. My belief is that the main difficulty with IHC for phospho-epitopes is not formalin fixation per se, but rather the instability of the phosphorylation. I used to work with rodent tumor models, where we collected solid tumors (or other tissues) just after sacrifice, and fixed them by formalin immersion. We found that the periphery of the tissues fixed this way expressed various phospho-epitopes quite well, but not the center. My interpretation was that by the time formalin had penetrated to the deep regions of the tissue, the phosphorylation had gone away (as you know formalin penetrates solid tissues fairly slowly). We proceeded to test matched tissue samples, fixed either in formalin at room temperature vs. cold formalin (formalin was at 4C at immersion, and the tissue was put in the fridge after sampling). We found that the cold formalin samples expressed phosphorylation sites fairly evenly, compared to only peripheral with the room temp samples. Presumably the cold temperature preserves the phosphorylation sites long enough to leave time for formalin to penetrate throughout. Consequently we integrated cold formalin fixation to all our IHC protocols for phospho-epitopes. Hope this helps, Jean-Martin Lapointe Accellab -----Original Message----- Good day colleagues, Does anyone have any information on phospho antibodies and fixation? Is there any reason to NOT fix specimens in formalin vs say, 70% EtOH? (and then process them through formalin later?) I can't seem to find any information on whether or not fixation in formalin does something strange to a phosphorylated protien and makes it a less accessible antigen. Also, on that same note, does retreival do anything to it? I am assuming that these antibodies go through the same testing for cross reactivity, etc (depending on the vendor) and are reliable when used properly (like any other antibody). I don't, however, know if there is enough of a difference in the epitope (the fact that it is phosphorylated) that would make it more susceptible to some strange cross linking with formalin (especiallly with phosphate buffered formalin). Any help with this topic would be greatly appreciated as I am uneducated in this area. Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 21 **************************************** From lpwenk <@t> sbcglobal.net Sun Apr 18 13:38:10 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Apr 18 13:38:15 2010 Subject: [Histonet] hematoxylin-eosin saffron In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0819ACAA@NTRSEVS30002.s3.ms.unilever.com> Message-ID: In a good H&E, the eosin/phloxine is supposed to be 3 shades: - RBC's/eosinophils/Paneth cells the deepest shade of pink - muscle medium shade of pink - collagen/connective tissue lightest shade of pink Unfortunately, if the eosin and/or phloxine and/or eosin/phloxine does not have the correct concentration of the eosin, pH of eosin, time in alcohols after eosin, etc, there ends up being 2 shades of eosin, with the collagen/CT and the muscle being the same shade. Adding saffron in the HPS gives the collagen/CT a more yellowish shade. So the tissue results are: - RBC's/eosinophils/Paneth cells the deepest shade of pink - muscle medium shade of pink - collagen/connective tissue yellowish with a tinge of pink Therefore, with the HPS, it's easier to differentiate muscle from collagen/CT. But I think most pathologists can diagnose with only 2 shades of eosin. If they need to differentiate collagen/CT from muscle (cirrhosis, tumors), they either ask for a trichrome or IHC. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Friday, April 16, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hematoxylin-eosin saffron Can someone please explain to me the purpose of a hematoxylin-eosin saffron stain. Why would this be used over the normal hematoxylin and eaosin stain. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akoug <@t> hotmail.com Sun Apr 18 17:08:35 2010 From: akoug <@t> hotmail.com (=?iso-8859-1?B?QW5kcukgS291Z2lvdW1vdXR6YWtpcw==?=) Date: Sun Apr 18 17:08:40 2010 Subject: [Histonet] Dark background with Gomori's reticulin impregnation Message-ID: Hi everyone, I've been performing Gomori's reticulin impregnation and never got the results I see in textbooks. The reticulin fibers stain fine, but I get a dark purple/gray background instead of a light pink background. Does anyone know why ? Here is the technique I use : 1) Deparaffinize and rehydrate 2) 0,5% potassium permanganate 1 min 3) Running tapwater 3 min 4) Distilled water 5) 5% Oxalic acid 2 min 6) Running tapwater 3 min 7) Distilled water 8) 2% ammonium iron sulfate 1 min 9) Running tapwater 3 min 10) Distilled water 11) Ammoniacal silver 1 min 12) Distilled water 15 sec 13) 20% Formaldehyde 3 min 14) Running tapwater 3 min 15) Distilled water 16) 0,2% Gold chloride 5 min 17) Distilled water 18) 3% potassium metabisuphite 5 min 19) Distilled water 20) Sodium thiosulfate 1 min 21) Running tapwater 3 min 22) Distilled water 23) 0,1% Nuclear fast red 15 min 24) Distilled water 25) Dehydrate, clear and mount Thanks for your help! Andre _________________________________________________________________ Videos that have everyone talking! Now also in HD! http://go.microsoft.com/?linkid=9724465 From laurie.reilly <@t> jcu.edu.au Sun Apr 18 18:16:50 2010 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Sun Apr 18 18:17:03 2010 Subject: [Histonet] hematoxylin-eosin saffron In-Reply-To: References: <0E6BC087F70F9C47ACFF2C203D6E329C0819ACAA@NTRSEVS30002.s3.ms.unilever.com> Message-ID: Dear Histonetters, We use an Eosin Y/Erythrosin B mixture containing calcium chloride in an aqueous solution, and this, if differentiated correctly in water, gives 3 definite shades of pink. Our students are asked to find an artery to check their differentiation and they should be able to see muscle, connective tissue and red blood cells in three shades of pink. This solution containing calcium chloride does need to be filtered before use. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, 19 April 2010 4:38 AM To: 'Kalleberg, Kristopher'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] hematoxylin-eosin saffron In a good H&E, the eosin/phloxine is supposed to be 3 shades: - RBC's/eosinophils/Paneth cells the deepest shade of pink - muscle medium shade of pink - collagen/connective tissue lightest shade of pink Unfortunately, if the eosin and/or phloxine and/or eosin/phloxine does not have the correct concentration of the eosin, pH of eosin, time in alcohols after eosin, etc, there ends up being 2 shades of eosin, with the collagen/CT and the muscle being the same shade. Adding saffron in the HPS gives the collagen/CT a more yellowish shade. So the tissue results are: - RBC's/eosinophils/Paneth cells the deepest shade of pink - muscle medium shade of pink - collagen/connective tissue yellowish with a tinge of pink Therefore, with the HPS, it's easier to differentiate muscle from collagen/CT. But I think most pathologists can diagnose with only 2 shades of eosin. If they need to differentiate collagen/CT from muscle (cirrhosis, tumors), they either ask for a trichrome or IHC. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Friday, April 16, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hematoxylin-eosin saffron Can someone please explain to me the purpose of a hematoxylin-eosin saffron stain. Why would this be used over the normal hematoxylin and eaosin stain. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BenatM <@t> gosh.nhs.uk Mon Apr 19 05:45:38 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Mon Apr 19 05:46:07 2010 Subject: [Histonet] Dark background with Gomori's reticulin impregnation In-Reply-To: References: Message-ID: <4BCC4264.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Andre, By looking at your method it does not look right from the start. We use the Gordon and Sweet Method and it work wonder for demonstration of reticulin fibers Here it is Hope it helps Malika 1. Sections to water. 2. Treat with 0.25% acidified Potassium permanganate. --- 5 mins 3. Bleach in 1% oxalic acid. 4. Rinse in distilled water. 5. Treat with 5% iron alum. --- 15 mins 6. Rinse briefly in distilled water. 7. Impregnate with ammoniacal silver solution. Filter Solution before use ---1 min (Note: Time in ammoniacal silver may need to be increased for bone marrow trephines) 8. Rinse well in distilled water. 9. Reduce in 10% formalin (in tap water). --- 1min 10. Rinse in distilled water. 11. Tone in 0.2% Gold Chloride ---1 min 12. Rinse in distilled water. 13. Fix in 5% sodium thiosulphate (hypo) --- 30 sec 14. Wash in water. 15. Counterstain with neutral red. 16. Dehydrate, clear and mount. Results Reticulin - Black Collagen - Grey Nuclei - Red > From: Andr? Kougioumoutzakis > Date: 18 April 2010 23:08:35 GMT+01:00 > To: > Subject: [Histonet] Dark background with Gomori's reticulin > impregnation > > > Hi everyone, > > > > I've been performing Gomori's reticulin impregnation and never got > the results I see in textbooks. The reticulin fibers stain fine, but > I get a dark purple/gray background instead of a light pink > background. Does anyone know why ? > > > > > > Here is the technique I use : > > > > 1) Deparaffinize and rehydrate > > 2) 0,5% potassium permanganate 1 min > > 3) Running tapwater 3 min > > 4) Distilled water > > 5) 5% Oxalic acid 2 min > > 6) Running tapwater 3 min > > 7) Distilled water > > 8) 2% ammonium iron sulfate 1 min > > 9) Running tapwater 3 min > > 10) Distilled water > > 11) Ammoniacal silver 1 min > > 12) Distilled water 15 sec > > 13) 20% Formaldehyde 3 min > > 14) Running tapwater 3 min > > 15) Distilled water > > 16) 0,2% Gold chloride 5 min > > 17) Distilled water > > 18) 3% potassium metabisuphite 5 min > > 19) Distilled water > > 20) Sodium thiosulfate 1 min > > 21) Running tapwater 3 min > > 22) Distilled water > > 23) 0,1% Nuclear fast red 15 min > > 24) Distilled water > > 25) Dehydrate, clear and mount > > > > Thanks for your help! > > Andre > > > > _________________________________________________________________ > Videos that have everyone talking! Now also in HD! > http://go.microsoft.com/?linkid=9724465_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From Karen.Heckford <@t> CHW.edu Mon Apr 19 06:57:56 2010 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Mon Apr 19 06:58:02 2010 Subject: [Histonet] Does anyone have and use Shandon Excelsior EX In-Reply-To: <15EEF32874DF4E6EB5F22368F2284AB1@DFS66DD1> References: <15EEF32874DF4E6EB5F22368F2284AB1@DFS66DD1> Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697F83@CHW-MSG-301.chw.edu> I have had one for a few years now and love it. You can also monitor it from another location if you have analog internet. It is really easy to change out the reagents and is way less messy than most other processors. You need to make sure your reagents stay topped off. The only real problems I have had with it is a Pathologist loading it wrong and not setting it down on the tongs correctly and breaking my basket that the cassettes set into. That was kind of a nightmare. I had to retrain the Pathologist. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Friday, April 16, 2010 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone have and use Shandon Excelsior EX Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Mon Apr 19 07:10:49 2010 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Mon Apr 19 07:14:28 2010 Subject: [Histonet] Processor needed Message-ID: <443F5B475A9BF647AB962E834884EBAD2788770DBE@EX7CCRPW03V1.chop.edu> Hi All, We are in need of a used tissue processor. If anyone has one for sale in the Philadelphia area, please email rosini@email.chop.edu. Budget is very small!!! Thank you. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF [Karen.Heckford@CHW.edu] Sent: Monday, April 19, 2010 7:57 AM To: Michelle MacVeigh-Aloni Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Does anyone have and use Shandon Excelsior EX I have had one for a few years now and love it. You can also monitor it from another location if you have analog internet. It is really easy to change out the reagents and is way less messy than most other processors. You need to make sure your reagents stay topped off. The only real problems I have had with it is a Pathologist loading it wrong and not setting it down on the tongs correctly and breaking my basket that the cassettes set into. That was kind of a nightmare. I had to retrain the Pathologist. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Friday, April 16, 2010 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone have and use Shandon Excelsior EX Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Mon Apr 19 07:46:26 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Apr 19 07:47:52 2010 Subject: [Histonet] Does anyone have and use Shandon Excelsior EX In-Reply-To: <15EEF32874DF4E6EB5F22368F2284AB1@DFS66DD1> Message-ID: Michelle, We have two Excelsiors and absolutely love them. The tech time saved on rotating solutions is well worth the cost. Solutions rotate out according to your specifications or alcohol content. Solutions are loaded during the next cycle. Tech time saved once again. We also are able to get more usage out of our reagents. Line up a demo and see for yourself. Great processors. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Michelle MacVeigh-Aloni" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/16/2010 03:51 PM To cc Subject [Histonet] Does anyone have and use Shandon Excelsior EX Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Rcartun <@t> harthosp.org Mon Apr 19 09:02:32 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 19 09:02:44 2010 Subject: [Histonet] Chlamydia testing Message-ID: <4BCC2A38.7400.0077.1@harthosp.org> I spoke with a patient recently who wanted his prostate biopsy tissue (from 2 years ago) tested for Chlamydia. Is anyone doing PCR for Chlamydia on formalin-fixed, paraffin-embedded tissue? I am reluctant to do IHC testing because my assay is not validated for prostate tissue. Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From making <@t> ufl.edu Mon Apr 19 09:24:38 2010 From: making <@t> ufl.edu (MKing) Date: Mon Apr 19 09:23:38 2010 Subject: [Histonet] phosphoantibodies Message-ID: <4BCC67A6.5040807@ufl.edu> Ashley, Phosphatases are relatively resistant to fixation which is why the histochemical phosphatase methods work. You may want to try putting phosphatase inhibitors in you fix or post-fix solutions to stop dephosphorylation, and see if it makes a difference in your ihc. I think Sigma has a table of reagents somewhere on their website and I'll email the link if I can find it. Good luck, Mike UF Pharmacology & Therapeutics ------------------------ Date: Fri, 16 Apr 2010 14:43:15 -0500 From: "Troutman, Kenneth A" Subject: [Histonet] Phospho antibodies and fixation To: "Histonet@lists.utsouthwestern.edu" Message-ID:<7B310892042DA74CB3590053F424CFE60B7480EFBC@ITS-HCWNEM06.ds.Vanderbilt.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone have any information on phospho antibodies and fixation?... Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 From pruegg <@t> ihctech.net Mon Apr 19 11:14:09 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Apr 19 11:14:47 2010 Subject: SPAM-LOW: [Histonet] phosphoantibodies In-Reply-To: <4BCC67A6.5040807@ufl.edu> References: <4BCC67A6.5040807@ufl.edu> Message-ID: <53C2449370194E208588549E501E2985@Patsyoffice> Here is a fixative we found in a paper on Phospho antibodies when we did a WS at NSH in 2007. 2.0mM EDTA 2.0mM EGTA 2.0mM Vanadate 40mM NaF 20mMpNPP(para-nitrophenyl phosphate) We used this fixative compared to routine 10% NBF and did not see any differences in IHC staining with phospho antibodies. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Monday, April 19, 2010 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] phosphoantibodies Ashley, Phosphatases are relatively resistant to fixation which is why the histochemical phosphatase methods work. You may want to try putting phosphatase inhibitors in you fix or post-fix solutions to stop dephosphorylation, and see if it makes a difference in your ihc. I think Sigma has a table of reagents somewhere on their website and I'll email the link if I can find it. Good luck, Mike UF Pharmacology & Therapeutics ------------------------ Date: Fri, 16 Apr 2010 14:43:15 -0500 From: "Troutman, Kenneth A" Subject: [Histonet] Phospho antibodies and fixation To: "Histonet@lists.utsouthwestern.edu" Message-ID:<7B310892042DA74CB3590053F424CFE60B7480EFBC@ITS-HCWNEM06.ds.Vande rbilt.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone have any information on phospho antibodies and fixation?... Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Mon Apr 19 11:21:37 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Mon Apr 19 11:21:44 2010 Subject: [Histonet] Does anyone have and use Shandon Excelsior EX In-Reply-To: <15EEF32874DF4E6EB5F22368F2284AB1@DFS66DD1> References: <15EEF32874DF4E6EB5F22368F2284AB1@DFS66DD1> Message-ID: <000501cadfdc$63bcd7c0$2b368740$@com> I have been using the Excelsior for over 4 yrs . Compared to the last VIP we had this is wonderful!! We save on Reagents by rotating on the first Alcohol quality. Very user friendly, Would suggest purchasing the Warranty & Netmon. A bit expensive but the PM & any parts alone pretty much cover that cost. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Friday, April 16, 2010 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone have and use Shandon Excelsior EX Hi all, According to the advertisement, this is supposed to be the most histotech friendly processor. Does anyone have a comment? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Mon Apr 19 12:40:46 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Apr 19 12:40:49 2010 Subject: [Histonet] retrieveing DNA from a mounted H&E slide Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C24@wlmmsx01.nemours.org> Hello histonetter's...I come one again to the experts in the field. I have been give two H&E slides from an autopsy case (have no idea how old)...and have been asked to retieve this tissue into an eppy with lysis buffer for DNA. Does anyone out there have an existing method for doing this? I have a notion what is needed to do this (though I am somewhat skeptical on the quality of the DNA retieved from the tissue) .....but, perfer not to re-invent the wheel...So if someone out there has a method for doing this, I would certainly appreciate it, if you wouldn't mind sharing it with us. Thanks... to all! CB From carl.hobbs <@t> kcl.ac.uk Mon Apr 19 12:57:13 2010 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon Apr 19 13:02:03 2010 Subject: [Histonet] Re: phosphoAbs on FFPW sections Message-ID: <11D9615B89C10747B1C985966A63D7CA2D7BAE97D5@KCL-MAIL04.kclad.ds.kcl.ac.uk> There are images here http://www.immunoportal.com/index.php in the Image gallery, of immunostaining results using a range of phospho-Abs if anyone cares to have a look, make comments etc.... best regards, carl From christina.thurby <@t> bms.com Mon Apr 19 13:03:25 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon Apr 19 13:03:40 2010 Subject: [Histonet] RE: Phospho antibodies In-Reply-To: <6ebc5403-ef43-49b0-98e9-05173a0be790@ushpwbmsmhp001.one.ads.bms.com> References: <6ebc5403-ef43-49b0-98e9-05173a0be790@ushpwbmsmhp001.one.ads.bms.com> Message-ID: Ashley, When we have worked with phospho antibodies, we have used the following pre-treatment to induce de-phosphorylation of the tissue sections: 1. _ Deparaffinize slides to distilled water. 2. _ Perform De-phosphorylation step. (Add 400 uL of alkaline phosphatase (Sigma Type VII-L) to 600 uL of 0.01 M PBS). Incubate for 2 hours at 32?C. Rinse well with distilled water. 3. _ _ PBS, 2-5 min. Proceed with your laboratory IHC staining protocol. (We use the ABC method as a standard). What antibody are you planning to use? I have a few protocols available for animal tissue on the Dako Autostainer. We fixed in either 10% NBF for routine necropsy specimens or PLP fixative for perfusion. ------------------------ >Date: Fri, 16 Apr 2010 14:43:15 -0500 >From: "Troutman, Kenneth A" >Subject: [Histonet] Phospho antibodies and fixation >To: "Histonet@lists.utsouthwestern.edu" > >Message-ID:<7B310892042DA74CB3590053F424CFE60B7480EFBC@ITS- >HCWNEM06.ds.Vanderbilt.edu> > Content-Type: text/plain; charset="iso-8859-1" >Does anyone have any information on phospho antibodies and fixation?... >Thanks, >Ashley Troutman BS, HT(ASCP) QIHC >Vanderbilt University Histopathology >1301 Medical Center Drive TVC 4532 >Nashville, TN 37232 >615-343-9134 > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Maxim_71 <@t> mail.ru Mon Apr 19 13:12:09 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Apr 19 13:12:16 2010 Subject: [Histonet] Dark background with Gomori's reticulin impregnation Message-ID: <403018085.20100419221209@mail.ru> Andre: 1. Red shadows in reticulin maybe as results too long steps with gold chloride. In your protocol it is for 5 mins. Try this step as 1 min. 2. Try substitute in step 13) 20% formaline at 10%. It can be prevent high gray background. 3. Chek pH of DW before nuclear fast red. It must be approx. 5-5.5. At reduction pH red conterstain will look as you have seen. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From badzrosari <@t> yahoo.com Mon Apr 19 15:03:54 2010 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Mon Apr 19 15:04:01 2010 Subject: [Histonet] Between supra & Thin prep Message-ID: <487434.64455.qm@web52108.mail.re2.yahoo.com> hi histonetters...dont have experience using the supra for cytology samples...any recommendation??? From dellav <@t> musc.edu Mon Apr 19 15:23:04 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Apr 19 15:23:16 2010 Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system In-Reply-To: <954307.19408.qm@web82308.mail.mud.yahoo.com> References: <954307.19408.qm@web82308.mail.mud.yahoo.com> Message-ID: If you prefer not to post to the list I'd appreciate hearing about your pros and cons off list as we will be evaluating this instrument. Thank you Loralee. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Bulmer Sent: Friday, April 16, 2010 9:40 AM To: JeanTaylor; 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu'; Loralee AMcMahon Subject: Re: [Histonet] RE: DAKO Autostainer Link 48/FLEX system Hey Loralee, ? Could you post a few of the pros and cons?? Do you like the new system? Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX --- On Thu, 4/15/10, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system To: "Taylor, Jean" , "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Date: Thursday, April 15, 2010, 8:12 PM I have upgraded to the new autostainer links.? I have four of them. There are many pros and many cons.? If you have any specific questions I'd be happy to answer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtaylor@meriter.com] Sent: Thursday, April 15, 2010 3:56 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DAKO Autostainer Link 48/FLEX system Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Apr 20 02:06:25 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Apr 20 02:06:30 2010 Subject: [Histonet] retrieveing DNA from a mounted H&E slide In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C24@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C24@wlmmsx01.nemours.org> Message-ID: Long, long ago when dinosaurs roamed and PCR was an emerging technique, what I would do is as follows: REmove coverslip in appropriate solvent, soaking te get any mounting media residue off. Rinse slide in abs alc and the 70% alcohol and then scrape off tissue into an Eppy with a new disposable blade. (Obviously, wearing gloves and taking care not to cut yourself). Rinse in sterile water, spin down, pur off supernatant , and contine as for normal extraction with your usual lysis buffer/extraction system. (back then we were using phenol chloroform!) The quality of the DNA is dependent on one of several factors, including the fixation medium, length of fixation, section thickness etc. On Mon, Apr 19, 2010 at 7:40 PM, Barone, Carol wrote: > Hello histonetter's...I come one again to the experts in the field. > > I have been give two H&E slides from an autopsy case (have no idea how > old)...and have been asked to retieve this tissue into an eppy with > lysis buffer for DNA. Does anyone out there have an existing method for > doing this? I have a notion what is needed to do this (though I am > somewhat skeptical on the quality of the DNA retieved from the tissue) > .....but, perfer not to re-invent the wheel...So if someone out there > has a method for doing this, I would certainly appreciate it, if you > wouldn't mind sharing it with us. Thanks... to all! CB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From meitanishi <@t> hotmail.com Tue Apr 20 03:58:20 2010 From: meitanishi <@t> hotmail.com (Amer Mustafa Gondolbeu) Date: Tue Apr 20 03:58:25 2010 Subject: [Histonet] Histological tissue ink problems Message-ID: Hello everyone, I'm doing histological sections of ulnar nerve to study the distribution of the fascicles. It's important the position of the section and for this reason I'm using histological tissue ink for mark the anterior, posterior, medial and lateral side of the section. The problem comes when I have to stain the sections (with luxol fast blue). The staining procedure eliminates my marks and I lose the orientation. How should I do to preserve the marks? Thanks! Macro and Microdissection Lab Anatomy and Embriology Unit Faculty of Medicine University of Barcelona _________________________________________________________________ Recibe un SMS de tu Hotmail vayas donde vayas. ?Date de alta! http://home.mobile.live.com/MobileAttach.mvc/?mkt=es-es From integrated.histo <@t> gmail.com Tue Apr 20 09:15:17 2010 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Tue Apr 20 09:15:21 2010 Subject: [Histonet] Need Microscope objective Message-ID: Does anyone know where we can purchase (new or used) a 45x objective for an old AO microscope? When we came in on Monday morning ours was cracked (?). Not sure how it happened since it was fine when we left on Friday. It is really hard to evaluate our bug stains w/o it. Cindy DuBois Integrated Pathology Stockton, CA From Patel.Payal <@t> synthes.com Tue Apr 20 09:22:08 2010 From: Patel.Payal <@t> synthes.com (Patel.Payal@synthes.com) Date: Tue Apr 20 09:22:16 2010 Subject: [Histonet] Histotechnician Position Message-ID: Job Posting Position: Histotechnician Location: West Chester, Pa Dear Histonetters, Synthes is advertising a full time permanent histotechnician position in our Biomaterials Division. We are looking for someone with one to three-years of relevant histology or pathology laboratory experience. A Bachelors or Associates degree in biochemistry, biotechnology, biology, chemistry, or equivalent combination of education, training and experience would be a required. If anyone is interested, please follow the link below and apply through the website. All applications must be processed through HR. http://us.synthes.com/CmsSynthes/openpositions.aspx From laurie.colbert <@t> huntingtonhospital.com Tue Apr 20 09:39:37 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Apr 20 09:39:43 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven Message-ID: <57BE698966D5C54EAE8612E8941D7683086D5A05@EXCHANGE3.huntingtonhospital.com> I'm curious as to whether or not others put their IHC slides on a hotplate after cutting? If so, at what temperature and for how long? Who only uses an oven - and what temp and for how long? Does anyone use both the hotplate and the oven? Laurie Colbert From azdudley <@t> hotmail.com Tue Apr 20 09:46:43 2010 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Apr 20 09:46:47 2010 Subject: [Histonet] new er pr cap guidelines Message-ID: has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 From mpence <@t> grhs.net Tue Apr 20 09:47:24 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Apr 20 09:47:30 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven In-Reply-To: <57BE698966D5C54EAE8612E8941D7683086D5A05@EXCHANGE3.huntingtonhospital.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DD1@is-e2k3.grhs.net> We use an oven only at 60c for 20 minutes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, April 20, 2010 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Slides, Hotplate vs Oven I'm curious as to whether or not others put their IHC slides on a hotplate after cutting? If so, at what temperature and for how long? Who only uses an oven - and what temp and for how long? Does anyone use both the hotplate and the oven? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Apr 20 10:06:39 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 20 10:06:49 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: References: Message-ID: <4BCD8ABE.7400.0077.1@harthosp.org> I believe they are posted on the CAP website. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> anita dudley 4/20/2010 10:46 AM >>> has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Apr 20 10:13:52 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Apr 20 10:22:33 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <4BCD8ABE.7400.0077.1@harthosp.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DD2@is-e2k3.grhs.net> Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, April 20, 2010 10:07 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines I believe they are posted on the CAP website. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> anita dudley 4/20/2010 10:46 AM >>> has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=P ID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5__________________________ _____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 20 10:24:15 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 20 10:24:23 2010 Subject: [Histonet] Need Microscope objective In-Reply-To: Message-ID: <535745.68682.qm@web65704.mail.ac4.yahoo.com> Try eBay. They usually have used objectives for sale. Ren? J. --- On Tue, 4/20/10, Cindy DuBois wrote: From: Cindy DuBois Subject: [Histonet] Need Microscope objective To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 20, 2010, 10:15 AM Does anyone know where we can purchase (new or used) a 45x objective for an old AO microscope?? When we came in on Monday morning ours was cracked (?).? Not sure how it happened since it was fine when we left on Friday. It is really hard to evaluate our bug stains w/o it. Cindy DuBois Integrated Pathology Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Apr 20 10:43:11 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Apr 20 10:43:17 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven In-Reply-To: <57BE698966D5C54EAE8612E8941D7683086D5A05@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683086D5A05@EXCHANGE3.huntingtonhospital.com> Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E16@UWHC-MAIL01.uwhis.hosp.wisc.edu> I sometimes put cut slides on a warm plate attached to my water bath. One of the plates is set at 55d and the other at 60d. I leave them till all the paraffin is completely melted. I only do this for cases I'm "paranoid" about staying on! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, April 20, 2010 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Slides, Hotplate vs Oven I'm curious as to whether or not others put their IHC slides on a hotplate after cutting? If so, at what temperature and for how long? Who only uses an oven - and what temp and for how long? Does anyone use both the hotplate and the oven? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Teri.Hallada <@t> midmichigan.org Tue Apr 20 10:43:35 2010 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Tue Apr 20 10:43:50 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DD2@is-e2k3.grhs.net> Message-ID: <8839B08E3ED7364E8CBBD53882C984D514102A62@MAILSRV01.midmichigan.net> Under the frequently asked questions on the CAP web site is the answer to Her2. It is still at 48 hours. Teresa Hallada BS, MT/CT (ASCP) Pathology Lead MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, April 20, 2010 11:14 AM To: Richard Cartun; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, April 20, 2010 10:07 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines I believe they are posted on the CAP website. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> anita dudley 4/20/2010 10:46 AM >>> has anyone seen the new er pr guidelines for er and pr? my pathologist just came through all in a tizzy about it. just wondering if anyone else has seen it. the fixation times were also suppose to change, to 72 hrs fixation for breast. thanks for anybodies input, anita, providence hosp. mobile alabama _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=P ID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5__________________________ _____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From relia1 <@t> earthlink.net Tue Apr 20 11:32:57 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 20 11:33:05 2010 Subject: [Histonet] Histotechnician Position In-Reply-To: Message-ID: Hello, I saw your posting online and thought I would offer my services. My name is Pam Barker and my company RELIA Solutions is a a permanent placement company that specializes in the nationwide recruitment of anatomic pathology professionals. I specialize in the area of histology recruiting. I have been a specialist in the area of histology recruiting for over 7 years. I have a wide network of histology professionals all over the U.S. I am aware of who among them is looking for a new position and who is considering a move. I know why they want to make a job change, where they want to live and what their credentials are. I can find you the right person for your lab. Let me save you time and money in your search for a histo tech. Put me and my resources to work for you. If you would like more information I can send you another e-mail containing our fee agreement and additional information about our services. Also from time to time I would like to send you information about some of the candidates I am most excited about. If you would like assistance with your immediate need or would like to discuss working with me in the future I would be happy to contact you or you can contact me at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patel.Payal@synthes.com Sent: Tuesday, April 20, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Cc: Cowan.Beth@synthes.com; Woods.Shane@synthes.com; Harten.Robert@synthes.com; DePaula.Alex@synthes.com Subject: [Histonet] Histotechnician Position Job Posting Position: Histotechnician Location: West Chester, Pa Dear Histonetters, Synthes is advertising a full time permanent histotechnician position in our Biomaterials Division. We are looking for someone with one to three-years of relevant histology or pathology laboratory experience. A Bachelors or Associates degree in biochemistry, biotechnology, biology, chemistry, or equivalent combination of education, training and experience would be a required. If anyone is interested, please follow the link below and apply through the website. All applications must be processed through HR. http://us.synthes.com/CmsSynthes/openpositions.aspx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcai <@t> prosci-inc.com Tue Apr 20 11:35:47 2010 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Tue Apr 20 11:35:54 2010 Subject: [Histonet] control background Message-ID: <001801cae0a7$888135d0$7d01a8c0@mic> Hi, I am doing multilabeling immunofluorescence now and try to get rid of the background staining around tissue. It might be due to the fluochrome attached to the glass around the tissue. Is there anyway to decrease or avoid this kind of staining? Thank you, Best, Karen From mpence <@t> grhs.net Tue Apr 20 12:10:08 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Apr 20 12:10:43 2010 Subject: [Histonet] Histotechnician Position In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DD3@is-e2k3.grhs.net> You should conduct your business off line! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Tuesday, April 20, 2010 11:33 AM To: Patel.Payal@synthes.com; histonet@lists.utsouthwestern.edu Cc: Cowan.Beth@synthes.com; Woods.Shane@synthes.com; Harten.Robert@synthes.com; DePaula.Alex@synthes.com Subject: RE: [Histonet] Histotechnician Position Hello, I saw your posting online and thought I would offer my services. My name is Pam Barker and my company RELIA Solutions is a a permanent placement company that specializes in the nationwide recruitment of anatomic pathology professionals. I specialize in the area of histology recruiting. I have been a specialist in the area of histology recruiting for over 7 years. I have a wide network of histology professionals all over the U.S. I am aware of who among them is looking for a new position and who is considering a move. I know why they want to make a job change, where they want to live and what their credentials are. I can find you the right person for your lab. Let me save you time and money in your search for a histo tech. Put me and my resources to work for you. If you would like more information I can send you another e-mail containing our fee agreement and additional information about our services. Also from time to time I would like to send you information about some of the candidates I am most excited about. If you would like assistance with your immediate need or would like to discuss working with me in the future I would be happy to contact you or you can contact me at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patel.Payal@synthes.com Sent: Tuesday, April 20, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Cc: Cowan.Beth@synthes.com; Woods.Shane@synthes.com; Harten.Robert@synthes.com; DePaula.Alex@synthes.com Subject: [Histonet] Histotechnician Position Job Posting Position: Histotechnician Location: West Chester, Pa Dear Histonetters, Synthes is advertising a full time permanent histotechnician position in our Biomaterials Division. We are looking for someone with one to three-years of relevant histology or pathology laboratory experience. A Bachelors or Associates degree in biochemistry, biotechnology, biology, chemistry, or equivalent combination of education, training and experience would be a required. If anyone is interested, please follow the link below and apply through the website. All applications must be processed through HR. http://us.synthes.com/CmsSynthes/openpositions.aspx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan <@t> mail.holyname.org Tue Apr 20 12:27:57 2010 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue Apr 20 12:28:27 2010 Subject: [Histonet] (no subject) Message-ID: Hi All, We are using Ventana XT for our immunostaining. We tried using "Plus" slides made by many vendors. The current vendor's pricing is way too high! With other vendors slides we have been having problems. Please let me know where to get some plus slides which are reasonable. I know the recommended slides come from erie but I am not quite sure about vendors who carry it and how much they charge. Thanks Nirmala Srishan Histology Holy Name Medical Center. __________________ Job Posting Position: Histotechnician Location: West Chester, Pa Dear Histonetters, Synthes is advertising a full time permanent histotechnician position in our Biomaterials Division. We are looking for someone with one to three-years of relevant histology or pathology laboratory experience. A Bachelors or Associates degree in biochemistry, biotechnology, biology, chemistry, or equivalent combination of education, training and experience would be a required. If anyone is interested, please follow the link below and apply through the website. All applications must be processed through HR. http://us.synthes.com/CmsSynthes/openpositions.aspx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From relia1 <@t> earthlink.net Tue Apr 20 12:32:18 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 20 12:32:23 2010 Subject: [Histonet] Re: HISTONET Histotechnician Position. My Apologies Message-ID: Hello Histonetters, I want to apologize for my "rookie" listserv mistake. I accidentally hit reply to all when I responded to a job posting. Please disregard that e-mail. And By the way... Happy Clinical Lab Professionals Week. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From NSEARCY <@t> swmail.sw.org Tue Apr 20 12:37:14 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Apr 20 12:37:21 2010 Subject: [Histonet] Lab Week Message-ID: <4BCD9FFA.5D38.00EF.0@swmail.sw.org> I hope all of you are having a great week. We had our CAP inspectors show yesterday, so we have had an interesting lab week. Needless to say we weren't expecting them on this week----- :) Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From nto <@t> stowers.org Tue Apr 20 12:49:02 2010 From: nto <@t> stowers.org (Thomas, Nancy) Date: Tue Apr 20 12:49:09 2010 Subject: [Histonet] Alcian blue alizarin red double stain Message-ID: Our lab has a request for alcian blue/alizarin red staining on mouse kidney teratomas. This will be done on FFPE slide mounted sections. In the past, we have done skeletal staining using these stains which also involved all of the clearing steps using potassium hydroxide. Has anyone done this stain (minus all of the clearing steps) on paraffin sections? If so, could I get a copy of your protocol? Thank you, Nancy From Mark.Elliott <@t> hli.ubc.ca Tue Apr 20 12:49:18 2010 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Apr 20 12:49:54 2010 Subject: [Histonet] Region IX Education Event May 28 and 29th Vancouver BC In-Reply-To: <0F18DCB4.794@mail.mrl.ubc.ca> References: <0F18DCB4.794@mail.mrl.ubc.ca> Message-ID: <4BCD86AE020000D60003DC6C@mail.mrl.ubc.ca> It's that time of the year again! Region IX is hosting their annual Education Event May 28 & 29 on the west coast in beautiful British Columbia. The meeting will be held at the Vancouver Hilton Metrotown in Burnaby (suburb of Vancouver). There are two days of great lectures planned and there will be between 10-14 vendors present showing us their latest and greatest. Full details, including hotel information, can be found on the Region IX Website (www.nshregionix.org). Hope to see you there! If you have any questions please feel free to contact me. Mark Elliott Region IX Education Chair ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From katherine-walters <@t> uiowa.edu Tue Apr 20 13:05:41 2010 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Tue Apr 20 13:06:43 2010 Subject: [Histonet] Alcian blue alizarin red double stain In-Reply-To: References: Message-ID: I would also be interested in the protocol. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas, Nancy Sent: Tuesday, April 20, 2010 12:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Alcian blue alizarin red double stain Our lab has a request for alcian blue/alizarin red staining on mouse kidney teratomas. This will be done on FFPE slide mounted sections. In the past, we have done skeletal staining using these stains which also involved all of the clearing steps using potassium hydroxide. Has anyone done this stain (minus all of the clearing steps) on paraffin sections? If so, could I get a copy of your protocol? Thank you, Nancy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Tue Apr 20 13:44:05 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Apr 20 13:44:09 2010 Subject: [Histonet] POSITION SOMERSET NJ Message-ID: <1504709228.19641951271789045459.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> We have a position available in Somerset NJ for a full time supervisor in?our soon to be constructed urology lab.? Duties include grossing, microwave, embedding, cutting and staining to include immuo stains.? You will also be responsible for preparing urine cytology.? Candidate must have 2 years experience. Interested candidates can send resume to: Coastal Pathology Consulting Services 267 722-8308 From joseph.tamasi <@t> bms.com Tue Apr 20 15:03:43 2010 From: joseph.tamasi <@t> bms.com (Tamasi, Joseph) Date: Tue Apr 20 15:03:50 2010 Subject: [Histonet] Region II Meeting Message-ID: <845938D0FDD6B54BAD926F60FF10A6A805CA459566@ushpwbmsmmp009.one.ads.bms.com> Registration is now open for the Region II Symposium to be held June 10-12, 2010 at the Clarion Hotel & Conference Center in Atlantic City West, New Jersey. For program and registration information contact Joe Tamasi, 609-818-3288, joseph.tamasi@bms.com. ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From thisisann <@t> aol.com Tue Apr 20 15:56:48 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Tue Apr 20 15:57:00 2010 Subject: [Histonet] CAP Checklist Message-ID: <8CCAEE3211E306A-1C94-185C@webmail-m053.sysops.aol.com> Can someone tell me where on the CAP website I can get a copy of the checklist to prepare for an inspection (for all departments, not just Histology...etc. Cytology, FISH, PCR). Thank you, Ann From AnthonyH <@t> chw.edu.au Tue Apr 20 18:18:37 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 20 18:18:51 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven In-Reply-To: <57BE698966D5C54EAE8612E8941D7683086D5A05@EXCHANGE3.huntingtonhospital.com> Message-ID: We use an oven (62oC) for 25min only. See: Henwood, A., (2005) "Effect of Slide Drying at 80?C on Immunohistochemistry" J Histotechnol 28(1):45-46. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, 21 April 2010 12:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Slides, Hotplate vs Oven I'm curious as to whether or not others put their IHC slides on a hotplate after cutting? If so, at what temperature and for how long? Who only uses an oven - and what temp and for how long? Does anyone use both the hotplate and the oven? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Apr 20 19:49:32 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Apr 20 19:50:28 2010 Subject: [Histonet] CAP Checklist In-Reply-To: <8CCAEE3211E306A-1C94-185C@webmail-m053.sysops.aol.com> References: <8CCAEE3211E306A-1C94-185C@webmail-m053.sysops.aol.com> Message-ID: They will send you a copy of the checklist that you will be inspected under before your inspection. They do not publish it that I know of. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Tuesday, April 20, 2010 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Checklist Can someone tell me where on the CAP website I can get a copy of the checklist to prepare for an inspection (for all departments, not just Histology...etc. Cytology, FISH, PCR). Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Tue Apr 20 21:36:21 2010 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Tue Apr 20 21:37:01 2010 Subject: [Histonet] Texas Society for Histotechnology Meeting April 22-25, 2010 Message-ID: <8CCAF128FD77CB5-18C-15A96@webmail-m071.sysops.aol.com> Hi Histonetters: It is not to late to register for the TSH meeting this weekend in Houston Texas at the Omni Hotel Westside. There will be 15 Workshops and 3 symposiums and many great vendor exhibits. We h ope to see you there. Book Signing Event There will be a book signing event during Friday?s President?s Reception at the Texas Society for Histotechnology Convention. Freida Carson and Christa Hladik will be available for discussions and to sign their book, Histotechnology A Self-Instructional Text. If you have a current copy of the text, feel free to bring it for the authors signature. If you?d like a copy, they will have the updated third edition available. Regards, TSH Convention Committee From k.r.gillinder <@t> newcastle.ac.uk Wed Apr 21 03:41:38 2010 From: k.r.gillinder <@t> newcastle.ac.uk (Kevin Gillinder) Date: Wed Apr 21 03:41:54 2010 Subject: [Histonet] Neonatal Mouse Brain Atlas In-Reply-To: <8CCAF128FD77CB5-18C-15A96@webmail-m071.sysops.aol.com> Message-ID: Hi Histo-lovers! I am planning on doing some nissl staining on neonatal mouse brain, to identify the trigeminal ganglion. Does anyone have a nissl stained atlas of mouse brain @ P0 (newborn) ? Has anyone had experience with this before? If so, would you mind sharing any tips? -- Kevin From BenatM <@t> gosh.nhs.uk Wed Apr 21 11:27:54 2010 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Wed Apr 21 11:28:26 2010 Subject: [Histonet] Chloroform Fume Monitoring Message-ID: <4BCF3599.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Hi Histoneter, I was just wondering what kind Health and Safety of procedures do people use to monitor Chloroform Fume ? I was carrying an H&S audit for occupational exposure Xylene / Glutaraldehyde / Formaldehyde/ Chloroforms fumes using Surgipath Exposures monitoring badge on a 3 months interval. when I realised that we do not have any Chloroform Badges available, and will not be able to get some for a little while. Does anyone use these EMT's Chloroform Monitoring Kit available to purchase from this site http://www.emt-online.com/ProductPages/Chloroform.htm Or is there any better alternative ? Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From WBENTON <@t> umm.edu Wed Apr 21 12:19:00 2010 From: WBENTON <@t> umm.edu (Walter Benton) Date: Wed Apr 21 12:19:12 2010 Subject: [Histonet] CAP Checklist In-Reply-To: References: Message-ID: <4BCEFB44020000F400057582@GWIA2.umm.edu> Message: 10 Date: Tue, 20 Apr 2010 16:56:48 -0400 From: thisisann@aol.com Subject: [Histonet] CAP Checklist To: histonet@lists.utsouthwestern.edu Message-ID: <8CCAEE3211E306A-1C94-185C@webmail-m053.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can someone tell me where on the CAP website I can get a copy of the checklist to prepare for an inspection (for all departments, not just Histology...etc. Cytology, FISH, PCR). Thank you, Ann Ann, You can access the checklist through www.cap.org under the Tab " Reference Resource and Publications" If you log in you will get the checklist for your facility. You can also purchase checklist as well on this website. Hope this helps. Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From WBENTON <@t> umm.edu Wed Apr 21 12:22:54 2010 From: WBENTON <@t> umm.edu (Walter Benton) Date: Wed Apr 21 12:23:00 2010 Subject: [Histonet] Charged Slides for IHC on Ventana In-Reply-To: References: Message-ID: <4BCEFC2E020000F400057587@GWIA2.umm.edu> Message: 2 Date: Tue, 20 Apr 2010 10:27:57 -0700 From: srishan@mail.holyname.org Subject: [Histonet] (no subject) To: histonet-bounces@lists.utsouthwestern.edu, Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, We are using Ventana XT for our immunostaining. We tried using "Plus" slides made by many vendors. The current vendor's pricing is way too high! With other vendors slides we have been having problems. Please let me know where to get some plus slides which are reasonable. I know the recommended slides come from erie but I am not quite sure about vendors who carry it and how much they charge. Thanks Nirmala Srishan Histology Holy Name Medical Center. Nirmala, We use Fisher's charged slides 12-550-15, which I'm sure are made by Erie. Pricing will depend upon your contract with a particular vendor. SurgiPath aka Leica has charged slides that work well also. If you want the etched Control Box Slides we purchase from Cardinal. Hope this helps! Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Timothy.Morken <@t> ucsfmedctr.org Wed Apr 21 12:27:04 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Apr 21 12:27:17 2010 Subject: [Histonet] Histology in the news.... Message-ID: <1AAF670737F193429070841C6B2ADD4C0181772F0A@EXMBMCB15.ucsfmedicalcenter.org> Along with the new CAP/ASCO ER/PR guidelines there was a front page article in the NY Times yesterday: "Cancer Fight: Unclear Tests for New Drug." It details the issues with interpreting Her2 staining and the continuing issue with disparate results from different labs when doing ER, PR and Her2 testing on the same sample. Nothing new to those in the field, but we are under continued scrutiny so better be sure your validation procedures are in order!! Tim Morken Supervisor, Histology / IHC UCSF Medical Center San Francisco, CA From making <@t> ufl.edu Wed Apr 21 13:01:10 2010 From: making <@t> ufl.edu (MKing) Date: Wed Apr 21 13:00:03 2010 Subject: [Histonet] trigeminal Message-ID: <4BCF3D66.4030309@ufl.edu> Kevin, This article has a nice photo showing mouse trigeminal ganglia. Google images is your friend-- Mike Nature Protocols 2, - 152 - 160 (2007) Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity Sacha A Malin, Brian M Davis & Derek C Molliver ----------- Message: 14 Date: Wed, 21 Apr 2010 09:41:38 +0100 From: Kevin Gillinder Subject: [Histonet] Neonatal Mouse Brain Atlas To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histo-lovers! I am planning on doing some nissl staining on neonatal mouse brain, to identify the trigeminal ganglion. Does anyone have a nissl stained atlas of mouse brain @ P0 (newborn) ? Has anyone had experience with this before? If so, would you mind sharing any tips? Kevin From gagnone <@t> KGH.KARI.NET Wed Apr 21 14:30:30 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Apr 21 14:30:38 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven Message-ID: Tony's answer (62 degrees C for 25 mins) is the minimum to ensure section adheres to slide. We all have to stain slides that have been baked longer than 30 mins. However, especially for Her2, longer baking times can result in false negatives or at least diminished staining. The justification for baking longer is an attempt to prevent the section washing off or lifting. We are seeing evidence that section adhesion is not a good tradeoff for diminished staining. Ideally both can be achieved with no tradeoff. Tony or others who may wish to answer: 1. How do you monitor the shortened baking time, especially when multiple racks of slides are cut throughout the day? 2. Assuming charged slides and cell conditioning/antigen retrieval are employed, are you seeing major problems with section adhesion? 3. What if slides are received from another institution for staining, or slides get baked longer than 25 mins? Thanks in advance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From mbbarra <@t> uol.com.br Wed Apr 21 15:14:52 2010 From: mbbarra <@t> uol.com.br (Marinez) Date: Wed Apr 21 15:14:52 2010 Subject: [Histonet] Bone marrow trephine decalcification Message-ID: <962D5FB303084D158BEB42636BF75FCB@MarinezNote> I'm in a bit of trouble. I work in the south of Brazil (Porto Alegre) for some years and now in my hospital a lot of bone marrow biopsies are being performed (leukemia and lymphomas). With decalcification with strong acids (nitric) I'm getting very poor results with the immunohistochemistry. I'm trying to use EDTA but we really are not getting speed or good results (we controled de pH but still precipitates). Could some one be kind enough to send me some formula (simple one please) that will work in reasonable time (24 or 48 hours) and perform well with immunohistochemistry? I would deeply grateful. M. Barra From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Apr 21 15:25:04 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Apr 21 15:26:12 2010 Subject: [Histonet] Bone marrow trephine decalcification In-Reply-To: <962D5FB303084D158BEB42636BF75FCB@MarinezNote> References: <962D5FB303084D158BEB42636BF75FCB@MarinezNote> Message-ID: They have commercial rapid decals that are formic acid based that do not affect the immunohistochemistry. But it does affect some of the enzyme histochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marinez [mbbarra@uol.com.br] Sent: Wednesday, April 21, 2010 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone marrow trephine decalcification I'm in a bit of trouble. I work in the south of Brazil (Porto Alegre) for some years and now in my hospital a lot of bone marrow biopsies are being performed (leukemia and lymphomas). With decalcification with strong acids (nitric) I'm getting very poor results with the immunohistochemistry. I'm trying to use EDTA but we really are not getting speed or good results (we controled de pH but still precipitates). Could some one be kind enough to send me some formula (simple one please) that will work in reasonable time (24 or 48 hours) and perform well with immunohistochemistry? I would deeply grateful. M. Barra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From malbenatti <@t> googlemail.com Wed Apr 21 15:33:48 2010 From: malbenatti <@t> googlemail.com (Malika Benatti) Date: Wed Apr 21 15:33:26 2010 Subject: [Histonet] Bone marrow trephine decalcification Message-ID: Goodings & Stewarts also known as Formic/Formaldehyde fluid (10% formic acid, 5% formalin) for 6 hours should allow decal of fix BMT trephine Bx. " ... Smile it confuses people ..." On 21 Apr 2010, at 21:14, "Marinez" wrote: > I'm in a bit of trouble. I work in the south of Brazil (Porto > Alegre) for some years and now in my hospital a lot of bone marrow > biopsies are being performed (leukemia and lymphomas). With > decalcification with strong acids (nitric) I'm getting very poor > results with the immunohistochemistry. I'm trying to use EDTA but we > really are not getting speed or good results (we controled de pH > but still precipitates). Could some one be kind enough to send me > some formula (simple one please) that will work in reasonable time > (24 or 48 hours) and perform well with immunohistochemistry? I would > deeply grateful. > > > > M. Barra > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Apr 21 15:44:30 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Apr 21 15:44:35 2010 Subject: [Histonet] tape coverships Message-ID: Hello all I have a question on tape coverslips. We received a couple tape coverslipped slides in for scanning and the tape is completely removed from the slide. I would normally just coverslip with glass but the tissue is on the tape coverslip and not on the slide. How can I re attach the tape to the slide, can I use xylene and regular mounting media? Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From mpence <@t> grhs.net Wed Apr 21 16:11:22 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 21 16:11:28 2010 Subject: [Histonet] tape coverships In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DD6@is-e2k3.grhs.net> Just put the slide in xylene and coverslip with the tape containing the tissue. The you could put a couple small drops of mounting media on for good measure. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, April 21, 2010 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tape coverships Hello all I have a question on tape coverslips. We received a couple tape coverslipped slides in for scanning and the tape is completely removed from the slide. I would normally just coverslip with glass but the tissue is on the tape coverslip and not on the slide. How can I re attach the tape to the slide, can I use xylene and regular mounting media? Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Apr 21 17:06:11 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Apr 21 17:06:27 2010 Subject: [Histonet] tape coverships In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DD6@is-e2k3.grhs.net> Message-ID: Thanks it worked Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, April 21, 2010 3:11 PM To: Liz Chlipala; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tape coverships Just put the slide in xylene and coverslip with the tape containing the tissue. The you could put a couple small drops of mounting media on for good measure. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, April 21, 2010 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tape coverships Hello all I have a question on tape coverslips. We received a couple tape coverslipped slides in for scanning and the tape is completely removed from the slide. I would normally just coverslip with glass but the tissue is on the tape coverslip and not on the slide. How can I re attach the tape to the slide, can I use xylene and regular mounting media? Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zodiac29 <@t> comcast.net Wed Apr 21 17:35:37 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Apr 21 17:35:39 2010 Subject: [Histonet] Toluidine blue Message-ID: <1006938523.16905101271889337266.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny From brandihiggins <@t> gmail.com Wed Apr 21 18:18:43 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Wed Apr 21 18:18:49 2010 Subject: [Histonet] ascp exam Message-ID: Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message boards saying people don't even recognize the material in a lot of the questions, and how the images of stains are of very poor quality. I am just wondering if most of the people who don't pass are people who think because they work in a lab they will be able to pass the exam, and as a result they don't study for it the first time. Or, is it really that difficult and many people study hard but still cannot pass it. Any comments welcome! Thanks! Brandi Higgins From AnthonyH <@t> chw.edu.au Wed Apr 21 18:24:04 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 21 18:24:20 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven In-Reply-To: Message-ID: Eric, Some of my thoughts on your discussion questions: 1. How do you monitor the shortened baking time, especially when multiple racks of slides are cut throughout the day? We have timers set for when each rack goes into the oven. This might be an issue for labs with larger batches (we are a Children's hospital) 2. Assuming charged slides and cell conditioning/antigen retrieval are employed, are you seeing major problems with section adhesion? No, not usually. Problem sections tend to be those that are inadequately fixed (& therefore inadequately processed), brain and bone sections. They may tend to lift. 3. What if slides are received from another institution for staining, or slides get baked longer than 25 mins? Again, it is difficult to know the conditions that the tissues have undergone. In-built controls, if present are invaluable. We use a BondMax immunostainer, so the antigen retrieval temperature and time are better controlled than is possible with the manual procedure (or when I do it!). I believe that incomplete fixation causes more problems for morphology and immunohistochemistry than is commonly appreciated. "Fix the fixing and most problems are solved" - Hows that for a sweeping statement! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Thursday, 22 April 2010 5:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Slides, Hotplate vs Oven Tony's answer (62 degrees C for 25 mins) is the minimum to ensure section adheres to slide. We all have to stain slides that have been baked longer than 30 mins. However, especially for Her2, longer baking times can result in false negatives or at least diminished staining. The justification for baking longer is an attempt to prevent the section washing off or lifting. We are seeing evidence that section adhesion is not a good tradeoff for diminished staining. Ideally both can be achieved with no tradeoff. Tony or others who may wish to answer: 1. How do you monitor the shortened baking time, especially when multiple racks of slides are cut throughout the day? 2. Assuming charged slides and cell conditioning/antigen retrieval are employed, are you seeing major problems with section adhesion? 3. What if slides are received from another institution for staining, or slides get baked longer than 25 mins? Thanks in advance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amosbrooks <@t> gmail.com Wed Apr 21 19:03:21 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 21 19:03:27 2010 Subject: [Histonet] Charged Slides Message-ID: Hi, If cost is a problem for charged slides, you could always silanize them yourself using whatever slides you want. It takes some time, (time=money) but the slides themselves are cheaper. There is a great description with procedures here: http://www.ihcworld.com/_technical_tips/prevent_section_fall.htm Good Luck, Amos Date: Tue, 20 Apr 2010 10:27:57 -0700 From: srishan@mail.holyname.org Subject: [Histonet] (no subject) To: histonet-bounces@lists.utsouthwestern.edu, Message-ID: < OF4A13F24E.175F330A-ON8525770B.005F84CC-8825770B.006FF3A0@holyname.org> Content-Type: text/plain; charset="US-ASCII" Hi All, We are using Ventana XT for our immunostaining. We tried using "Plus" slides made by many vendors. The current vendor's pricing is way too high! With other vendors slides we have been having problems. Please let me know where to get some plus slides which are reasonable. I know the recommended slides come from erie but I am not quite sure about vendors who carry it and how much they charge. Thanks Nirmala Srishan Histology Holy Name Medical Center. From CIngles <@t> uwhealth.org Wed Apr 21 20:14:13 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Apr 21 20:14:35 2010 Subject: [Histonet] Toluidine blue References: <1006938523.16905101271889337266.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: Newcomer Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of zodiac29@comcast.net Sent: Wed 4/21/2010 5:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Apr 21 20:23:25 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Apr 21 20:23:29 2010 Subject: [Histonet] ascp exam References: Message-ID: Are you kidding!?! I have an AA in Histotechnology and still didn't pass my (HTL) written on the first round. I did pass my slide review though, thank you very much. :) I passed on the second try, which included 3 years of OJT and tons of studying after graduating school. (BTW, I also have a BS in Biology with a major in English) I think they have just made the test harder because they have gotten rid of the slide part of the test. And it sounds like the micrographs still suck. :p Claire Disclaimer: Chill out people. I'm not crabby, I'm just drawn that way. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Brandi Higgins Sent: Wed 4/21/2010 6:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ascp exam Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message boards saying people don't even recognize the material in a lot of the questions, and how the images of stains are of very poor quality. I am just wondering if most of the people who don't pass are people who think because they work in a lab they will be able to pass the exam, and as a result they don't study for it the first time. Or, is it really that difficult and many people study hard but still cannot pass it. Any comments welcome! Thanks! Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Apr 22 07:42:12 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Apr 22 07:42:20 2010 Subject: [Histonet] Happy Medical Laboratory Professionals Week from Pam Barker at RELIA! Message-ID: Hi Histonetters! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I hope your lab has been having some fun, informative and special events to celebrate because you deserve it.I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you get a chance check out Advance Magazine?s coverage of Lab Week on Facebook. You don?t have to belong to Facebook to see it. Here is the link: http://www.facebook.com/ADVANCEMedLab Also I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. I also wanted to let you know about the great job opportunities I am currently working on. I have supervisor and lead positions in Fresno, California, Boston area, Massachusetts, Las Vegas, NV and Long Island, NY I have histotechnician/histotechnologist positions in Los Angeles, CA,Corpus Christi, TX, Orange/Rockland County, NY, Cape Cod and Boston area of MA. I also have PA positions in Charlotte, NC and Las Vegas, NV. All of my positions are full time permanent positions My clients offer excellent compensation, benefits and relocation assistance. If you or anyone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or at relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia From jcampbell <@t> vdxpathology.com Thu Apr 22 08:16:42 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Thu Apr 22 08:16:48 2010 Subject: [Histonet] Fluoro Jade B (or C) protocol Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8386CA2@VDXSERVER01.vdxpathology.local> Hi All, Does anyone have an IHC protocol for Fluoro Jade B (or C)? I have read it can be adapted from FITC to IHC but I can't find a protocol. Thanks, Jennifer Campbell From brett_connolly <@t> merck.com Thu Apr 22 09:58:54 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Apr 22 09:59:02 2010 Subject: [Histonet] need tips for cross-sectioning of cortical bone Message-ID: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> A colleague is having trouble getting wrinkle-free sections of decalcified, paraffin embedded femur. Any tips?? Thanks, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From shshaw <@t> WPI.EDU Thu Apr 22 10:06:05 2010 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Thu Apr 22 10:06:16 2010 Subject: [Histonet] Frozen Rat Heart Message-ID: Does anybody have a protocol on freezing rat heart that you can share with me. Thanks, Sharon From anonwums1 <@t> gmail.com Thu Apr 22 10:12:26 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Apr 22 10:12:32 2010 Subject: [Histonet] need tips for cross-sectioning of cortical bone In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> Message-ID: Cutting bone is very hard, and I'm by no means an expert at it. Assuming the blocks are properly fixed and decalcified, the best thing I've found is to put the blocks at -20C for 5-10 mins to cool them, then right before you cut them, rub a little ice water on the face of the block. That should help you get some nice clean cuts. If the sections become hard to cut again, reapply the ice water. If that stops working, back in the freezer they go. Adam On Thu, Apr 22, 2010 at 9:58 AM, Connolly, Brett M wrote: > A colleague is having trouble getting wrinkle-free sections of > decalcified, paraffin embedded femur. > > Any tips?? > > Thanks, > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at http://www.merck.com/contact/contacts.html) > that may be confidential, proprietary copyrighted and/or legally privileged. > It is intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Thu Apr 22 10:19:18 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Apr 22 10:19:26 2010 Subject: [Histonet] Frozen Rat Heart In-Reply-To: References: Message-ID: <8C1FE0F4B6C8E5D8B51551C7@CDYwxp1931.ad.med.buffalo.edu> Hi Sharon, 1. Stuff the tissue in a cryovial or wrap it in foil and throw it in liquid N. 2. Put the tissue in a cryomold with OCT covering it and either place it on some frozen isopentane; put it in a slurry of isopentane and dry ice pieces; or suspend over liquid N vapors to freeze. Not sure if this is what you were looking for, precisely? Regards, Merced --On Thursday, April 22, 2010 11:06 AM -0400 "Shaw, Sharon" wrote: > Does anybody have a protocol on freezing rat heart that you can share > with me. Thanks, > Sharon > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From cmiller <@t> physlab.com Thu Apr 22 10:22:22 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 22 10:22:27 2010 Subject: [Histonet] ascp exam In-Reply-To: References: Message-ID: It is that difficult. I studied for months and months. While I passed the first time there were some difficult questions. I felt it was because a good part of what I needed to know I had no practical experience to draw from. Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Timothy.Morken <@t> ucsfmedctr.org Thu Apr 22 10:39:34 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Apr 22 10:39:44 2010 Subject: [Histonet] ascp exam In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4C01818AB7ED@EXMBMCB15.ucsfmedicalcenter.org> When I took the HT in 1989 I had studied for a year on my own and with a once-a-week study group from our lab. I learned on the job and had been working in histology for 5 years by that time and it definitely helps to do the practical work to get the details into your head (luckily our small lab was a regional lab and so did a large variety of procedures). Even so, we spent a LOT of time detailing out the differences between different types of stains, for instance, silver stains. What is similar and different between them and why. I read through Sheehan and the AFIP manual many times, went through the NSH study guides many times each, found every study guide I could, sometimes poor copies that had been handed down from tech to tech through the years (nothing "online" in those days!). When I took the HTL in 1992 it was a bit easier since it mostly covers HT material, but I still made the effort to review everything and then add in the management topics. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Wednesday, April 21, 2010 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ascp exam Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message boards saying people don't even recognize the material in a lot of the questions, and how the images of stains are of very poor quality. I am just wondering if most of the people who don't pass are people who think because they work in a lab they will be able to pass the exam, and as a result they don't study for it the first time. Or, is it really that difficult and many people study hard but still cannot pass it. Any comments welcome! Thanks! Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debra.Ortiz <@t> uchospitals.edu Thu Apr 22 10:45:29 2010 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Thu Apr 22 10:46:15 2010 Subject: [Histonet] RE: Histonet Digest, Vol 77, Issue 22 In-Reply-To: <201004181704.o3IH42KM021247@uchsmtp02> References: <201004181704.o3IH42KM021247@uchsmtp02> Message-ID: <5392DB699B157E4C8E65B3779634BD7D01847AD3@uchmbx04-hpk03s.UCHAD.uchospitals.edu> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, April 18, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 77, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: hematoxylin-eosin saffron (Robert Richmond) 2. Phospho antibodies and fixation (Jean-Martin Lapointe) ---------------------------------------------------------------------- Message: 1 Date: Sat, 17 Apr 2010 13:27:21 -0400 From: Robert Richmond Subject: [Histonet] Re: hematoxylin-eosin saffron To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The hematoxylin-phloxine-saffron (HPS) stain is a trichrome stain that came into use as a "general oversight" stain by surgical pathologists in the 1930's, notably at Columbia-Presbyterian Hospital in New York City. The Goldner-Foot trichrome stain was also used for this purpose, on the other side of Central Park at Cornell Medical Center, probably abandoned after Chandler Foot's retirement in 1948. I actually saw the HPS stain in use at Columbia-Presbyterian around 1966, in place of H & E. I understand that it is still in very limited use in a few laboratories today. Saffron (a natural product, the stigmas of the flowers of Crocus sativus) is extremely expensive - currently $66 for a quarter ounce (about 7 grams) at Penzeys.com. It's used histologically as an alcoholic extract (supposedly best done with a reflux condenser) and can actually be purchased in that form. You can find the HPS staining procedure in older books, such as the AFIP manual. As a working surgical pathologist, I find this ancient technique intriguing but totally impractical in today's world. It's stretching it to get a trichrome stain for your liver biopsies these days. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 17 Apr 2010 16:47:48 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] Phospho antibodies and fixation To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Ashley, I did a little bit of work on this a few years back. My belief is that the main difficulty with IHC for phospho-epitopes is not formalin fixation per se, but rather the instability of the phosphorylation. I used to work with rodent tumor models, where we collected solid tumors (or other tissues) just after sacrifice, and fixed them by formalin immersion. We found that the periphery of the tissues fixed this way expressed various phospho-epitopes quite well, but not the center. My interpretation was that by the time formalin had penetrated to the deep regions of the tissue, the phosphorylation had gone away (as you know formalin penetrates solid tissues fairly slowly). We proceeded to test matched tissue samples, fixed either in formalin at room temperature vs. cold formalin (formalin was at 4C at immersion, and the tissue was put in the fridge after sampling). We found that the cold formalin samples expressed phosphorylation sites fairly evenly, compared to only peripheral with the room temp samples. Presumably the cold temperature preserves the phosphorylation sites long enough to leave time for formalin to penetrate throughout. Consequently we integrated cold formalin fixation to all our IHC protocols for phospho-epitopes. Hope this helps, Jean-Martin Lapointe Accellab -----Original Message----- Good day colleagues, Does anyone have any information on phospho antibodies and fixation? Is there any reason to NOT fix specimens in formalin vs say, 70% EtOH? (and then process them through formalin later?) I can't seem to find any information on whether or not fixation in formalin does something strange to a phosphorylated protien and makes it a less accessible antigen. Also, on that same note, does retreival do anything to it? I am assuming that these antibodies go through the same testing for cross reactivity, etc (depending on the vendor) and are reliable when used properly (like any other antibody). I don't, however, know if there is enough of a difference in the epitope (the fact that it is phosphorylated) that would make it more susceptible to some strange cross linking with formalin (especiallly with phosphate buffered formalin). Any help with this topic would be greatly appreciated as I am uneducated in this area. Thanks, Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 21 **************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 22 **************************************** ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From rjr6 <@t> psu.edu Thu Apr 22 10:46:27 2010 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Apr 22 10:46:51 2010 Subject: [Histonet] ascp exam In-Reply-To: References: Message-ID: When I took the HT exam I was in a histology program with on the job work as the students helped in the lab and didn't have any problem passed first time. Several years ago when I decided to take the HTL I took a week off work and packed the dog and tent and went camping with all my books and study guides. I would go over everything for hours then take the dog for a hike then start over again. I passed first time then too. I thought the pictures in the second test were really bad. For the HT I had actual photos if I remember correctly and they were much clearer. Roberta Horner HT/HTL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Wednesday, April 21, 2010 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ascp exam Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message boards saying people don't even recognize the material in a lot of the questions, and how the images of stains are of very poor quality. I am just wondering if most of the people who don't pass are people who think because they work in a lab they will be able to pass the exam, and as a result they don't study for it the first time. Or, is it really that difficult and many people study hard but still cannot pass it. Any comments welcome! Thanks! Brandi Higgins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Thu Apr 22 10:52:57 2010 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Apr 22 10:53:02 2010 Subject: [Histonet] Toluidine blue In-Reply-To: References: Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE86102037FA56@VEXCCR02.phsabc.ehcnet.ca> I get mine from Sigma-Aldrich, it's pretty cheap for the dry chemical. Message: 12 Date: Wed, 21 Apr 2010 22:35:37 +0000 (UTC) From: zodiac29@comcast.net Subject: [Histonet] Toluidine blue To: histonet@lists.utsouthwestern.edu Message-ID: <1006938523.16905101271889337266.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny From lmoffatt <@t> susquehannahealth.org Thu Apr 22 11:09:12 2010 From: lmoffatt <@t> susquehannahealth.org (Moffatt, Loretta) Date: Thu Apr 22 11:08:40 2010 Subject: [Histonet] contamination Message-ID: Has anyone had any issues with cross contamination during the embedding process. What do other histology labs do to clean forceps during the embedding process? What steps are taken during grossing to prevent specimen contamination? Loretta Moffatt, MT(ASCP),MHA Laboratory Manager 777 Rural Avenue Williamsport, PA 17701 570-321-2326 (F)570-321-2489 lmoffatt@susquehannahealth.org When one door closes another opens; but we so often look so long and so regretfully upon the closed door, that we do not see the ones which open for us. Confidentiality Notice: This message and any attachments originate by electronic mail from Susquehanna Health System and their subsidiaries/affiliates ("SHS"). Both this document and any attachments are intended for the sole use of the addressee indicated above and may contain proprietary, privileged and/or confidential information. If you are not the intended recipient of this message, you are hereby notified that any use or disclosure of this information is strictly prohibited. If you received this message in error, or have reason to believe you are not authorized to receive it, please notify the sender by reply email, with a copy to ITSecurity@susquehannahealth.org < mailto:ITSecurity@susquehannahealth.org>, and then promptly delete the original and reply messages. Thank you for your cooperation. From Maxim_71 <@t> mail.ru Thu Apr 22 11:54:37 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Apr 22 11:54:55 2010 Subject: [Histonet] new er pr cap guidelines Message-ID: <1986998504.20100422205437@mail.ru> Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513?520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru From Timothy.Morken <@t> ucsfmedctr.org Thu Apr 22 12:05:32 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Apr 22 12:05:45 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <1986998504.20100422205437@mail.ru> References: <1986998504.20100422205437@mail.ru> Message-ID: <1AAF670737F193429070841C6B2ADD4C01818AB8CF@EXMBMCB15.ucsfmedicalcenter.org> Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Thu Apr 22 12:13:40 2010 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Apr 22 12:13:49 2010 Subject: [Histonet] IHC blocking for liver tissue Message-ID: <979759.70017.qm@web38006.mail.mud.yahoo.com> Hello, ? I have a question that what is best way to block endougenous biotin and HRP in liver or kidney tissue. The method I am using has to use avidin-biotin and HRP reactions. This is fine on heart tissue.? I used biotin blocking kit from DAKO or Vector. Also I used 1% H2O2. But liver tissue still show background stain. ? Could you teach me a better way to block or is there other reason cause background? ? Thanks in advance, ? Amy From LSebree <@t> uwhealth.org Thu Apr 22 12:41:53 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Apr 22 12:42:01 2010 Subject: [Histonet] Frozen Rat Heart In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738399E1A@UWHC-MAIL01.uwhis.hosp.wisc.edu> Sharon, I used to freeze rabbit hearts (a LITTLE bigger than rat) this way. Pour isopentane into a metal beaker inside a styrofoam container filled with liquid nitrogen to part way up the sides of the beaker. When the isopentane starts to freeze on the bottom of the beaker it is ready. Place cross sections of fresh tissue (or the whole rat heart) on a round of cork with gum tragacanth, orienting it to the way you may be sectioning it if that's its destiny. With a LONG forceps or hemostat, lower cork round, heart side up, into the isopentane. It's important to lower the tissue slowly to prevent cracking but not so slow as to cause freeze artifact. Complete freezing will only take a couple minutes if that. To section tissue, if that's the next step, freeze the bottom of the cork round onto a chuck using OCT. In this way, you insulate the tissue from the RT or cold OCT preventing any chance of thawing of your tissue. Hope this helps you in your situation, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Thursday, April 22, 2010 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Rat Heart Does anybody have a protocol on freezing rat heart that you can share with me. Thanks, Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Thu Apr 22 12:53:40 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Apr 22 12:53:46 2010 Subject: [Histonet] ASCP exam In-Reply-To: <20100422170235.722656825C@mail.pa-ucl.com> References: <20100422170235.722656825C@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC0644165A2B8DB@hestia.ad.pa-ucl.com> I was an MLT who transitioned into histology, did the program at IUPUI and passed the HT exam on the first try (Thankfully). I took the exam as soon as could following the program, studied Carson, exams from the program and purchased the online tests through ASCP. My coworker started in the gross room, then moved to cytoprep person and then into histology, did the program at IUPUI and did not pass the first time. We decided she had tried to study TOO many things, and she passed the second time. We talked about the tests and her second test was completely different from the first. I had asked her about the pictures and certain things I had remembered, and she did not have any of that on the first go round. We now have another student who will be sitting for the BOR this summer - we will see where that goes! Nancy Schmitt MLT/HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Wednesday, April 21, 2010 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ascp exam Hello, For those of you who are ASCP certified, either HT or HTL, did many of you have to take the exam more than once? I have not yet sat for the exam, but I see the pass rate is low, and average score is usually just slightly above the pass line. I have read a lot of comments on some message boards saying people don't even recognize the material in a lot of the questions, and how the images of stains are of very poor quality. I am just wondering if most of the people who don't pass are people who think because they work in a lab they will be able to pass the exam, and as a result they don't study for it the first time. Or, is it really that difficult and many people study hard but still cannot pass it. Any comments welcome! Thanks! Brandi Higgins _______________________________________________ *************************************** NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jcampbell <@t> vdxpathology.com Thu Apr 22 13:01:07 2010 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Thu Apr 22 13:01:12 2010 Subject: [Histonet] reagent alcohol in 50-55 gal drums Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8386CC2@VDXSERVER01.vdxpathology.local> Hi All, Does anyone out there purchase their reagent alcohol in 50-55 gal drums? How much does this cost you, including the shipping & handling and any other costs? We are trying to determine whether this would be a good idea or not. Thank you, Jennifer Campbell From mcauliff <@t> umdnj.edu Thu Apr 22 14:33:22 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 22 14:31:00 2010 Subject: [Histonet] Toluidine blue In-Reply-To: <1006938523.16905101271889337266.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1006938523.16905101271889337266.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <4BD0A482.2030509@umdnj.edu> Just be sure you get dye certified by the Biological Stain Commission. The bottle must have a Certification label or it is not certified to perform as expected. Sigma is reliable. Geoff zodiac29@comcast.net wrote: > Hello to all, > > > I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. > > > Thank you in advance for your replies, > > > Jenny > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From ebreisch <@t> rchsd.org Thu Apr 22 14:48:26 2010 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Thu Apr 22 14:48:34 2010 Subject: [Histonet] protein concentration for Cy2 Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B303A530F6@e2k3backend1.RCHSD.org> This question if for anyone doing C4d immunoflourescence staining. Could someone share what protein concentration the Cy2 labeled anti-mouse IgG is prepared at and what buffer is used for that? Our final dilution of the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original protein concentration for the dry Cy2 should be. Thank you for your assistance it is greatly appreciated. Eric A. Breisch From burch007 <@t> mc.duke.edu Thu Apr 22 15:12:52 2010 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Apr 22 15:13:05 2010 Subject: [Histonet] protein concentration for Cy2 In-Reply-To: <43B97B4C402C2C44AAA2A8D2C86A88B303A530F6@e2k3backend1.RCHSD.org> Message-ID: Eric: Are you using Cy2 labeled goat anti-mouse IgG (H&L) from Jackson ImmunoResearch Labs? According to their catalog (product number 115-225-003), the stock is 2.0 mg/ml. At 1:100, your working concentration would be 20 ug/ml. Jim B "Breisch, Eric" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/22/2010 03:51 PM To cc Subject [Histonet] protein concentration for Cy2 This question if for anyone doing C4d immunoflourescence staining. Could someone share what protein concentration the Cy2 labeled anti-mouse IgG is prepared at and what buffer is used for that? Our final dilution of the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original protein concentration for the dry Cy2 should be. Thank you for your assistance it is greatly appreciated. Eric A. Breisch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Thu Apr 22 17:12:04 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Thu Apr 22 17:12:10 2010 Subject: [Histonet] need tips for cross-sectioning of cortical bone In-Reply-To: References: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> Message-ID: <152586.45708.qm@web113804.mail.gq1.yahoo.com> Brett- Most wrinkles in decalcified bone sections come from stretching of the decalified bone that occurs during the sectioning process.? I would suggest a rather simple solution.? Allowing the sections to flatten on the waterbath might take longer or a higher temperature.? If paraffin surrounding the bone section seems to be containing it, not allowing it to expand to eliminate the wrinkles, gently tease it off.? After all, you want the bone section, not the paraffin.? A room temperature water bath (or 30% EtOH) to lay out the sections?on to?tease out any wrinkles before transfering the sections to the warm waterbath may also help. I hope this helps! Joe Saby, BA HT ________________________________ From: Adam . To: "Connolly, Brett M" Cc: histonet@lists.utsouthwestern.edu Sent: Thu, April 22, 2010 11:12:26 AM Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone Cutting bone is very hard, and I'm by no means an expert at it. Assuming the blocks are properly fixed and decalcified, the best thing I've found is to put the blocks at -20C for 5-10 mins to cool them, then right before you cut them, rub a little ice water on the face of the block. That should help you get some nice clean cuts. If the sections become hard to cut again, reapply the ice water. If that stops working, back in the freezer they go. Adam On Thu, Apr 22, 2010 at 9:58 AM, Connolly, Brett M wrote: > A colleague is having trouble getting wrinkle-free sections of > decalcified, paraffin embedded femur. > > Any tips?? > > Thanks, > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > > > Notice:? This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at http://www.merck.com/contact/contacts.html) > that may be confidential, proprietary copyrighted and/or legally privileged. > It is intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zodiac29 <@t> comcast.net Thu Apr 22 17:41:19 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Thu Apr 22 17:41:22 2010 Subject: [Histonet] thank you Message-ID: <1621329660.17364841271976079691.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Thank you to everyone who responded to my question regarding where to purchase Toluidine blue. It is very much appreciated. Jenny From godsgalnow <@t> aol.com Thu Apr 22 20:02:16 2010 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Apr 22 20:02:24 2010 Subject: [Histonet] Need IHC Tech in Ny Message-ID: <1570708574-1271984537-cardhu_decombobulator_blackberry.rim.net-1753197452-@bda2106.bisx.prod.on.blackberry> I need a strong IHC tech with a NY license in Long Island. Great pay. Sent from my Verizon Wireless BlackBerry From 14558580 <@t> sun.ac.za Fri Apr 23 06:52:12 2010 From: 14558580 <@t> sun.ac.za (Burger, MC <14558580@sun.ac.za>) Date: Fri Apr 23 06:52:35 2010 Subject: [Histonet] Positive Controls - IHC Message-ID: Good day, I am desperately looking for positive controls for Immunohistochemistry testing. I am testing lung tissue for Adenovirus and RSV, but I need a positive control to be able to do this. Since it's not something easily obtained, I tried making cell blocks by using the pellet from a known positive cell culture, but this didn't work. Is it possible to buy known positive blocks and if so, where can I do this? I am new in the field of histology, so I don't know enough to find this out! Thank you in advance! Kind regards, Marilize Burger MScMedSc in Medical Virology Department of Pathology Stellenbosch University Faculty of Health Sciences PO Box 19063 Tygerberg Campus 7505 Cape Town South Africa From pruegg <@t> ihctech.net Fri Apr 23 08:25:45 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Apr 23 08:26:29 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <1AAF670737F193429070841C6B2ADD4C01818AB8CF@EXMBMCB15.ucsfmedicalcenter.org> References: <1986998504.20100422205437@mail.ru> <1AAF670737F193429070841C6B2ADD4C01818AB8CF@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Apr 23 09:16:39 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 23 09:16:44 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DDB@is-e2k3.grhs.net> This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Apr 23 09:21:39 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Apr 23 09:24:05 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DDB@is-e2k3.grhs.net> References: , <661949901A768E4F9CC16D8AF8F2838C017A3DDB@is-e2k3.grhs.net> Message-ID: I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Fri Apr 23 09:56:48 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Apr 23 09:57:03 2010 Subject: [Histonet] IHC Slides, Hotplate vs Oven Message-ID: Tony, thanks for your very helpful common-sense approach to answering my questions. It seems that baking time needs to be as standardized, well-controlled and well-documented as other steps in the IHC process. Especially given the importance of standardizing results in predictive and prognostic markers. Thanks, Eric From mpence <@t> grhs.net Fri Apr 23 09:58:02 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 23 09:58:07 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DDC@is-e2k3.grhs.net> My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays. -----Original Message----- From: McMahon, Loralee A [mailto:Loralee_Mcmahon@URMC.Rochester.edu] Sent: Friday, April 23, 2010 9:22 AM To: Mike Pence; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Apr 23 10:04:35 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Apr 23 10:04:49 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DDB@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3DDB@is-e2k3.grhs.net> Message-ID: <1AAF670737F193429070841C6B2ADD4C01818ABC49@EXMBMCB15.ucsfmedicalcenter.org> Yes, since there is still a difference between ER/Pr and Her2 recommended fixation times (ER/Pr at 72, Her2 at 48 hours) the defacto max time for ER/Pr is still 48 hours. They say they are studying the issue.... Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, April 23, 2010 7:17 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Fri Apr 23 10:17:04 2010 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Fri Apr 23 10:17:09 2010 Subject: [Histonet] ER clone 1D5 or SP1 ? Message-ID: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From SDrew <@t> uwhealth.org Fri Apr 23 10:19:20 2010 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Fri Apr 23 10:19:28 2010 Subject: [Histonet] ER clone 1D5 or SP1 ? In-Reply-To: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> Message-ID: <738A7878143FF74BB77436E255743C1A010004F4@UWHC-MAIL03.uwhis.hosp.wisc.edu> We are using 6F11... Sally -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ER clone 1D5 or SP1 ? I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Apr 23 10:29:31 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Apr 23 10:29:41 2010 Subject: [Histonet] new er pr cap guidelines In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DDC@is-e2k3.grhs.net> References: , <661949901A768E4F9CC16D8AF8F2838C017A3DDC@is-e2k3.grhs.net> Message-ID: In those situations you can set up your processors to hold in formalin until the time limit is reached and then switch to 70% Sample comes in on Friday. Place it in formalin (on the processor) until the 48 hours have been reached most likely Sunday afternoon, then switch to an extended 70% alcohol, then continue to process so your cassettes will come off on Monday morning. It might mean that you have a 6-8 hour 70% step, but it should be ok. OF COURSE, run some test tissues first to make sure. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: Mike Pence [mpence@grhs.net] Sent: Friday, April 23, 2010 10:58 AM To: McMahon, Loralee A; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays. -----Original Message----- From: McMahon, Loralee A [mailto:Loralee_Mcmahon@URMC.Rochester.edu] Sent: Friday, April 23, 2010 9:22 AM To: Mike Pence; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley > Subject: [Histonet] new er pr cap guidelines > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- ? ?????????, Maxim mailto:Maxim_71@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Fri Apr 23 10:53:16 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Apr 23 10:53:31 2010 Subject: [Histonet] ER clone 1D5 or SP1 ? Message-ID: <1615935799.20100423195316@mail.ru> Jean: We using clone 1D5 (Dako) since 2008 year with very good results. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From marktarango <@t> gmail.com Fri Apr 23 11:21:50 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 23 11:21:57 2010 Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> Message-ID: SP1 On Fri, Apr 23, 2010 at 8:17 AM, Taylor, Jean wrote: > > > I?m wondering which clone of ER most labs are using? > > > > Thanks, > > Jean Taylor, HT(ASCP)QIHC > > IHC Tech > > Meriter Health Services > > Madison, WI > From lucy.zong <@t> gmail.com Fri Apr 23 11:39:07 2010 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Fri Apr 23 11:39:10 2010 Subject: [Histonet] disposable blade holder Message-ID: Oh Budget cuts again! I been checking the web for a used disposable blade holder for the Microm HM 310 Microtome with no luck. Does anyone have an extra one laying around that I can purchase cheaply? . From GDawson <@t> dynacaremilwaukee.com Fri Apr 23 11:51:08 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Apr 23 11:51:12 2010 Subject: [Histonet] ER clone 1D5 or SP1 ? In-Reply-To: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> Message-ID: SP-1 here as well Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ER clone 1D5 or SP1 ? I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> carisdx.com Fri Apr 23 12:37:21 2010 From: mturner <@t> carisdx.com (Turner, Mark) Date: Fri Apr 23 12:37:25 2010 Subject: [Histonet] ER clone 1D5 or SP1 ? Message-ID: We use the SP1 clone here. Mark Turner, HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mturner@carisdx.com From collette2 <@t> mail.llnl.gov Fri Apr 23 12:50:23 2010 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Fri Apr 23 12:50:40 2010 Subject: [Histonet] need tips for cross-sectioning of cortical bone In-Reply-To: <152586.45708.qm@web113804.mail.gq1.yahoo.com> References: <63EA0607835FBA4689CEA9EA8B48269202FFDF8D@usctmx1141.merck.com> <152586.45708.qm@web113804.mail.gq1.yahoo.com> Message-ID: Hi, All, I primarily section bone, and it's usually paraffin. I second the vote on chilling the blocks (I chill mine in ice water instead of the freezer, so if I forget my blocks the wax doesn't crack!), and using ice water on a swab to keep the block chilled as you section. Generally I section the entire bone, so about halfway through I need to re-chill the block, but this whole chilling business may be processing regimen- and wax type-dependent (uh, and the proficiency of the user!). If you get a lot of compression of your ribbons, you will get a lot of wrinkles on your slides, so optimize your cutting for compression. If you look in the histonet archives, Rene Buesa posted some time ago typical causes for ribbon/section compression- I printed it out and keep it posted above the microtome to refer to when I'm at a loss ;) ... I transfer my sections to a room temp waterbath with some ethanol before transferring to the heated waterbath. I also leave my sections on the heated waterbath, usually 39-40C is a good temp for me, and let them float until they look pretty much wrinkle-free by eye, generally a few minutes. Having charged slides also helps, as the cartilage in your section wants to stick to itself rather than a slide, that's where a lot of the wrinkles are. A charged slide makes it want to stick to the slide rather than itself a little more. Last, after vertical air drying at room temp overnight (it's just convenient for me, but a few hours should suffice), I "bake" my slides at 45C overnight on a slide warmer, sometimes it's the downstream processing that gives you the wrinkles, and good adhesion helps, which is what the 'baking" is for. Keep in mind that I'm self-taught, and a very mouse biology small lab, I do all the processing, cutting, and staining myself. So, what works for me may not be feasible for everyone, and may still not be the best way to do things (although I do try to generate beautiful data). Sincerely, Nicole Collette LLNL/ UC Berkeley At 3:12 PM -0700 4/22/10, Joseph Saby wrote: >Brett- > >Most wrinkles in decalcified bone sections come from stretching of >the decalified bone that occurs during the sectioning process. I >would suggest a rather simple solution. Allowing the sections to >flatten on the waterbath might take longer or a higher temperature. >If paraffin surrounding the bone section seems to be containing it, >not allowing it to expand to eliminate the wrinkles, gently tease it >off. After all, you want the bone section, not the paraffin. A >room temperature water bath (or 30% EtOH) to lay out the sections on >to tease out any wrinkles before transfering the sections to the >warm waterbath may also help. > >I hope this helps! > >Joe Saby, BA HT > > > > >________________________________ >From: Adam . >To: "Connolly, Brett M" >Cc: histonet@lists.utsouthwestern.edu >Sent: Thu, April 22, 2010 11:12:26 AM >Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone > >Cutting bone is very hard, and I'm by no means an expert at it. Assuming the >blocks are properly fixed and decalcified, the best thing I've found is to >put the blocks at -20C for 5-10 mins to cool them, then right before you cut >them, rub a little ice water on the face of the block. That should help you >get some nice clean cuts. If the sections become hard to cut again, reapply >the ice water. If that stops working, back in the freezer they go. > >Adam > >On Thu, Apr 22, 2010 at 9:58 AM, Connolly, Brett M > wrote: > >> A colleague is having trouble getting wrinkle-free sections of >> decalcified, paraffin embedded femur. >> >> Any tips?? >> >> Thanks, >> >> Brett M. Connolly, Ph.D. >> Molecular Imaging Team Leader >> Merck & Co., Inc. >> PO Box 4, WP-44K >> West Point, PA 19486 >> tel. 215-652-2501 fax. 215-993-6803 > > brett_connolly@merck.com >> >> >> >> Notice: This e-mail message, together with any attachments, contains >> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New >> Jersey, USA 08889), and/or its affiliates Direct contact information for >> affiliates is available at http://*www.*merck.com/contact/contacts.html) >> that may be confidential, proprietary copyrighted and/or legally privileged. >> It is intended solely for the use of the individual or entity named on this >> message. If you are not the intended recipient, and have received this >> message in error, please notify us immediately by reply e-mail and then >> delete it from your system. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://*lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://*lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://*lists.utsouthwestern.edu/mailman/listinfo/histonet From erweber <@t> maxhealth.com Fri Apr 23 13:18:42 2010 From: erweber <@t> maxhealth.com (Eric Weber) Date: Fri Apr 23 13:18:52 2010 Subject: [Histonet] Contract Histo Tech Needed in Albuquerque, NM Message-ID: My name is Eric Weber and I am a recruiter for Maxim Government Services. We provide temporary contracted lab personnel to the federal medical facilities. I'm current recruiting for a contract Histology Technologist for an immediate start through August 13th, 2010. Details: * Special consideration given to candidates with previous experience in a Veteran Affairs (VA) Hospital. * Special consideration given to candidate local to the Albuquergue area * Prefer ASCP certification * Monday through Friday, 7am until 3:30 pm * No call, no overtime * Two year experience required cutting and Hematoxylin and Eosin staining. * Housing/travel benefits provide for non local candidates If you are interested please contact me immediately. Please indicate if you have previous experience in a VA Medical Center. Thank You, Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erweber@maxhealth.com ________________________________ CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate you forwarding the message back to us and deleting it from your system. Thank you. This email and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. From anonwums1 <@t> gmail.com Fri Apr 23 13:22:01 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Apr 23 13:22:04 2010 Subject: [Histonet] Two antibodies from the same host with tyramide Message-ID: Hi all, For the past several months, I have been attempting to get double immunofluorescence with two goat anti-mouse antibodies on mouse FFPE bone. The antibody that is giving me a lot of trouble is the goat anti-VE-Cadherin from R&D Systems. Essentially, it seems to work without the tyramide but when you do the entire procedure, it stops working. Here is my procedure: 1. Block peroxide (3% H2O2) 2. Block with TNB buffer (the blocking buffer that comes with the tyramide kit) 3. Block avidin/biotin 4. Goat anti-mouse antibody at 1:800. It's undetectable at this titer with a directly conjugated secondary. 5. Biotinylated donkey anti-goat 6. SA-HRP 7. Biotinyl tyramide 8. SA-594 9. Block with 10 ug / mL irrelevant goat IgG to bind up any leftover secondary 10. goat anti-VE-Cadherin 11. Anti-goat 649 Unfortunately, I never get any VE-cadherin staining this way. However, if I do a single stain using that antibody and its secondary, it works great. I've verified that when staining VE-Cadherin alone, TNB buffer and 10 ug / mL of goat IgG doesn't alter the staining. Next up are testing peroxidase block and avidin/biotin blocking, but after that I'll run out of ideas. I've also tried postponing the SA-HRP and the rest until after all primary and secondary antibodies are on there, and it still doesn't work. I'm pretty baffled. Thanks, Adam From Joyce.Cline <@t> wchsys.org Fri Apr 23 14:35:18 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Fri Apr 23 14:47:01 2010 Subject: [Histonet] accessioning of specimens Message-ID: We seem to be having trouble with the correct printed cassettes being placed on the correct containers. Or wrong numbers printed on cassettes. How does everyone accession their cases? Guidelines for checking cassettes & containers? Any suggestions other than looking over their shoulder with each case or going behind them and checking everything? AP assistants handle logging all our cases. (not HT's) Thank you Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Apr 23 15:17:15 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Apr 23 15:19:24 2010 Subject: [Histonet] RE: ER clone 1D5 or SP1 ? Message-ID: <7B310892042DA74CB3590053F424CFE60B8054FFC5@ITS-HCWNEM06.ds.Vanderbilt.edu> We have used 6F11 in the past and have switched to SP1. In running parallel tests, we noticed about a 97% concordance between the two. Good luck. Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, April 23, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 77, Issue 29 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ER clone 1D5 or SP1 ? I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ From Margaret.Perry <@t> sdstate.edu Fri Apr 23 16:15:58 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Apr 23 16:17:37 2010 Subject: [Histonet] new DAB Message-ID: When did DAKO retire their DAB and only stock the DAB plus? Since it's Friday I can rant. I am so disgusted that no one was notified of the change. With QC I'm going to have to revalidate or prove that the New DAB works as well as or better than the old. Of course I had to be a klutz and spill all but 3 ml of the old DAB buffer and I'll be at a conference all next week with no time to work up a new protocol. My pathologists are going to be upset if their stains don't look right. Don't companies understand we need to know about these changes before they happen? Especially something as common to all labs as DAB! Margaret Perry From JWeems <@t> sjha.org Fri Apr 23 16:19:54 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Apr 23 16:19:59 2010 Subject: [Histonet] RE: new DAB In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DCCBE5D@CHEXCMS10.one.ads.che.org> Bless you... Call you tech support and they will come do it for you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, April 23, 2010 17:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new DAB When did DAKO retire their DAB and only stock the DAB plus? Since it's Friday I can rant. I am so disgusted that no one was notified of the change. With QC I'm going to have to revalidate or prove that the New DAB works as well as or better than the old. Of course I had to be a klutz and spill all but 3 ml of the old DAB buffer and I'll be at a conference all next week with no time to work up a new protocol. My pathologists are going to be upset if their stains don't look right. Don't companies understand we need to know about these changes before they happen? Especially something as common to all labs as DAB! Margaret Perry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Margaret.Perry <@t> sdstate.edu Fri Apr 23 16:25:30 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Apr 23 16:25:34 2010 Subject: [Histonet] Gram stain Message-ID: Now that I'm done with my rant I have a real question. We are trying to do a gram stain on fish and the safranine O is staining everything red. What other stain would you use? I usually have time to look at the books but unfortunately it's almost quiting time and the slides need to be done by noon Monday. Thanks Margaret Perry From amylee779 <@t> yahoo.com Fri Apr 23 16:59:45 2010 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Fri Apr 23 16:59:48 2010 Subject: [Histonet] Thank you all sho gave me advice on reducing background of liver tissue! Message-ID: <742194.18970.qm@web38007.mail.mud.yahoo.com> From pathmaster <@t> yahoo.com Sat Apr 24 12:28:55 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Sat Apr 24 12:28:59 2010 Subject: [Histonet] Patient ID on cassettes Message-ID: <690158.71961.qm@web111108.mail.gq1.yahoo.com> To conform to CAP and state regulations that require two unique patient identifiers on a specimen at all analytical steps, and to cope with a persnickety cassette labeller that is down more than up, we have taken to writing the patient's first and last initials in the upper left corner of the writing surface of the cassette, above the S10- xxxx. Our pencil sharpeners are running non -stop? but it works. Only problem is when a block is sent out for IHC the returned slides are labelled with the initials as part of the reference slides accession numbers and my boss doesn't care for that. Originally we dropped the S in S10- and replaced with the initials to get a little more room, now? we added back the S and placed initials above S10-. Patient safety dictates that a prossector verify a match among? the labelled cassette and the labelled specimen containers and against the requisition and the number dictated into the gross computer/transcriber. Case by case, on every case. There is no substitute. As for placing the wrong cassettes on the wrong specimen containers, that seems to me to be pure carelessness and a disciplinary issue if it continues. When labelling, matching the initials that the microtomist writes on the slide with the patient's name printed on the slide labels provides a final? ID double check if the numbers on the slide get transcribed incorrectly. The initials thing takes minimal effort, but you need to be able to write small LOL. Jeff Silverman. From Maxim_71 <@t> mail.ru Sat Apr 24 14:08:48 2010 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Apr 24 14:08:55 2010 Subject: [Histonet] Gram stain Message-ID: <573298402.20100424230848@mail.ru> Margaret: We uses 1% aqueous neutral red as red counterstain with very good results. Do not forget rinse slides at 10-15 secs in DW with 1-2 drop of glacial acetic acid before neutral red and after this, because this dye have red color at pH < 6. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Fri, 23 Apr 2010 16:25:30 -0500 > From: "Perry, Margaret" > Subject: [Histonet] Gram stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Now that I'm done with my rant I have a real > question. We are trying to do a gram stain on fish > and the safranine O is staining everything red. What > other stain would you use? I usually have time to > look at the books but unfortunately it's almost > quiting time and the slides need to be done by noon Monday. Thanks > Margaret Perry mailto:Maxim_71@mail.ru From patpxs <@t> gmail.com Sun Apr 25 09:18:13 2010 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Sun Apr 25 09:18:19 2010 Subject: [Histonet] Best books for the HTL? Message-ID: Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab From Rcartun <@t> harthosp.org Sun Apr 25 14:04:28 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Apr 25 14:04:39 2010 Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> Message-ID: <4BD459FB.7400.0077.1@harthosp.org> I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en From amosbrooks <@t> gmail.com Sun Apr 25 18:14:49 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Apr 25 18:14:54 2010 Subject: [Histonet] Region 1 conference Message-ID: Hi, I would like to ask if anyone has any photos of the Region 1 conference to consider emailing them to me. I was going to post some on the Region 1 Conference web page and possibly put some in the Paraffin Press. If you are interested, please drop me a line. Thanks, Amos amosbrooks@gmail.com From ancillarypath <@t> mac.com Sun Apr 25 19:36:18 2010 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Sun Apr 25 19:36:26 2010 Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <4BD459FB.7400.0077.1@harthosp.org> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> <4BD459FB.7400.0077.1@harthosp.org> Message-ID: <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com> When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: > I have looked at several clones over the years and I prefer clone > 6F11. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" 4/23/2010 11:17 AM >>> > > I'm wondering which clone of ER most labs are using? > > Thanks, > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > > -- > Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en > From lurim <@t> challiance.org Mon Apr 26 06:23:57 2010 From: lurim <@t> challiance.org (Urim, Lyudmila) Date: Mon Apr 26 06:24:04 2010 Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain Message-ID: <6774152B580A6E44B38487B24A331E7C131603E0@sasquatch.cphc.local> > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. > Also on the affected GI biopsies there is often only 1 or 2 spots of > the biopsy that are affected while the rest of the biopsy looks fine. > The artifact is being reproduced on each cut; also the artifact > doesn't disappear after the affected biopsies get reprocessed. > The artifact can be described as a foggy, smudgy stain, with deformed > nucleus and not enough cell details. > All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy From Margaret.Perry <@t> sdstate.edu Mon Apr 26 07:43:53 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Apr 26 07:44:00 2010 Subject: [Histonet] paraformaldayhde Message-ID: In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? From JWeems <@t> sjha.org Mon Apr 26 08:48:08 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Apr 26 08:48:32 2010 Subject: [Histonet] RE: Mysterious artifact on GI biopsies H&E stain In-Reply-To: <6774152B580A6E44B38487B24A331E7C131603E0@sasquatch.cphc.local> References: <6774152B580A6E44B38487B24A331E7C131603E0@sasquatch.cphc.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DCCBF11@CHEXCMS10.one.ads.che.org> I would check to see what medication the patient was on or if something was done during the procedure to affect the biopsies.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 07:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. > Also on the affected GI biopsies there is often only 1 or 2 spots of > the biopsy that are affected while the rest of the biopsy looks fine. > The artifact is being reproduced on each cut; also the artifact > doesn't disappear after the affected biopsies get reprocessed. > The artifact can be described as a foggy, smudgy stain, with deformed > nucleus and not enough cell details. > All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From gankam <@t> googlemail.com Mon Apr 26 09:00:32 2010 From: gankam <@t> googlemail.com (Fabrice gankam) Date: Mon Apr 26 09:00:49 2010 Subject: [Histonet] anti 8 hydroxyguanosine Message-ID: Hey guys was wondering if any of you used the anti 8 hydroxyguanosine to detect free radical induced DNA and RNa damage. which antibody is the best. we tried the one from abdserotec and it is a disaster. the background is just horrible. any idea ? 2010/4/26 Perry, Margaret > In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of > powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mpence <@t> grhs.net Mon Apr 26 09:04:43 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Apr 26 09:04:48 2010 Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain In-Reply-To: <6774152B580A6E44B38487B24A331E7C131603E0@sasquatch.cphc.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DDF@is-e2k3.grhs.net> It could be a "hot biopsy"? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 6:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. Also on the affected > GI biopsies there is often only 1 or 2 spots of the biopsy that are > affected while the rest of the biopsy looks fine. The artifact is > being reproduced on each cut; also the artifact doesn't disappear > after the affected biopsies get reprocessed. The artifact can be > described as a foggy, smudgy stain, with deformed nucleus and not > enough cell details. All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Mon Apr 26 09:31:50 2010 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Mon Apr 26 09:31:54 2010 Subject: [Histonet] Any Histology openings in San Diego area? Message-ID: <519989.77668.qm@web56803.mail.re3.yahoo.com> Hi Netters, I have a friend looking to relocate to San Diego area and is looking for a Histology position, she is certified HT. Any help would be greatly appreciated, thanks in advance!! Jill Jill Cox HT (ASCP) Arizona Dermatology 4232 E Cactus Rd Phoenix AZ 85032 From lurim <@t> challiance.org Mon Apr 26 10:10:08 2010 From: lurim <@t> challiance.org (Urim, Lyudmila) Date: Mon Apr 26 10:10:13 2010 Subject: [Histonet] (no subject) Message-ID: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy From TJJ <@t> stowers.org Mon Apr 26 10:16:26 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Apr 26 10:16:32 2010 Subject: [Histonet] Re: Two antibodies from the same host with tyramide Message-ID: Adam, Thanks for the detailed protocol. I can't explain it either but I'll throw some ideas out there and maybe it'll stimulate discussion. Hopefully Chris vanderLoos will chime in. Have you tried doing the labeling in reverse, using the VE-Cadherin first and then blocking with the goat IgG, and then doing the other antibody/tyramide protocol? I'm not sure what effect, if any, the hydrogen peroxide might have on the anti-goat fluorescent 649 label, so you could certainly do that block prior to doing the first primary antibody incubation. Also, you may have to double the concentration of the Cadherin to get it to label at the same intensity as you do in single staining. But still I might have expected to see a signal. Do the two antibodies co-express? Could there be something with the covalent binding of the HRP-tyramide complex that shelters the antigenic sites for the second antibody? That's why I wondered what might happen if you reversed your protocol. Let us know if you get it figured out and what fixed it for you. Good luck, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From kryan <@t> nfderm.com Mon Apr 26 10:25:50 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Mon Apr 26 10:25:55 2010 Subject: [Histonet] (no subject) In-Reply-To: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> References: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> Message-ID: I highly recommend the Milestone microwave tissue processors. I have used their microwave tissues processors in two different locations and have found them to be very reliable, user friendly, reproducible and cost effective. They also have wonderful tech support. Kaye Ryan Histology Supervisor North Florida Dermatology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 11:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Mon Apr 26 10:49:08 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Apr 26 10:49:33 2010 Subject: [Histonet] (no subject) In-Reply-To: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> References: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> Message-ID: It really depends on your volume to be processed and what you are planning to process. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 10:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Timothy.Morken <@t> ucsfmedctr.org Mon Apr 26 10:54:23 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Apr 26 10:54:34 2010 Subject: [Histonet] Best books for the HTL? In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4C01818AC169@EXMBMCB15.ucsfmedicalcenter.org> Paula, Carson's book is probably them most relevant to basic histology these days (Sheehans is still very good for detail on technique and stains but has a lot of old material that you will rarely, if ever, see anymore). Bancroft's book is also excellent, has more detail on given stains and has more on management (QC, etc). For the HTL I also recommend the ASCP book "Quality Management in Anatomic Pathology" by Nakhleh and Fitzgibbons. Plus the usual NSH study guides. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Sunday, April 25, 2010 7:18 AM To: HistoNet Subject: [Histonet] Best books for the HTL? Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Mon Apr 26 11:02:43 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Apr 26 11:02:49 2010 Subject: [Histonet] (no subject) In-Reply-To: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6277@EXCHANGECLUSTER.yumaregional.local> I would look at this very carefully, especially in light of the new regulation that were just released by the CAP on ER and PR. But that is up to each individual lab Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 8:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From deb.vaneyck <@t> phci.org Mon Apr 26 11:29:34 2010 From: deb.vaneyck <@t> phci.org (Van Eyck, Deb) Date: Mon Apr 26 11:30:00 2010 Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> <4BD459FB.7400.0077.1@harthosp.org> <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com> Message-ID: <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From pruegg <@t> ihctech.net Mon Apr 26 11:46:09 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Apr 26 11:46:48 2010 Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> <4BD459FB.7400.0077.1@harthosp.org> <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com> <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> Message-ID: Did I understand correctly from Dr. Hammond in Florida that the ER ASCO/CAP guidelines extended the fixation time to 72 hours? Are they changing the Her2 guidelines to match the ER? If so, has that happened yet? I have people very anxious to stop having techs work on the weekends to comply with the 48 hour fixation limits. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Monday, April 26, 2010 10:30 AM To: ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From ancillarypath <@t> mac.com Mon Apr 26 12:03:47 2010 From: ancillarypath <@t> mac.com (Hadi Yaziji) Date: Mon Apr 26 12:03:55 2010 Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> <4BD459FB.7400.0077.1@harthosp.org> <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com> <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> Message-ID: Patsy and all of the wonderful technical colleagues, The ER guidelines are already in print, and you can download the article from the CAP website. Yes, you are correct about the upper fixation limit for ER, which was adopted from our consensus conference that included a number of experienced scientists (Yaziji H, et al. Applied Immunohistochemistry Dec 2008). Regarding HER2 guidelines, DO NOT CHANGE YOUR TECHNICAL STAFF SCHEDULE YET. The panel is meeting now to deliberate on increasing the upper limit of fixation for HER2 to 72 hours. We are pushing hard on this, but 1/2 of the panel is made of medical oncologists who do not know much about tissue fixation. Once the changes are made, I promise to share the news with the groups first thing if they don't get it through CAP publication. Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 26, 2010, at 12:46 PM, Patsy Ruegg wrote: > Did I understand correctly from Dr. Hammond in Florida that the ER > ASCO/CAP guidelines extended the fixation time to 72 hours? Are > they changing the Her2 guidelines to match the ER? If so, has that > happened yet? I have people very anxious to stop having techs work > on the weekends to comply with the 48 hour fixation limits. > > Thank you, > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for the use of the > Person(s) ('the intended recipient') to whom it was addressed. Any > views or opinions presented are solely those of the author. It may > contain information that is privileged & confidential within the > meaning of applicable law. Accordingly any dissemination, > distribution, copying, or other use of this message, or any of its > contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly > prohibited. If you are NOT the intended recipient please contact the > sender and dispose of this e-mail as soon as possible. > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On > Behalf Of Van Eyck, Deb > Sent: Monday, April 26, 2010 10:30 AM > To: ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver > Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? > > This is a great discussion lets also talk about PR clones since the > ASCO/CAP guidelines just came out ------Hadi or Rich I know they > only list two PR clones one is Dako 1294-----what is the other 312? > Deb > > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On > Behalf Of ancillarypath@mac.com > Sent: Sunday, April 25, 2010 7:36 PM > To: ihcrg Group (E-mail); histonet netserver > Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? > > When we started our lab 3 years ago, we began with SP1 from day 1, > so I don't have any experience with either 1D5 or 6F11 except in my > previous labs. 1D5 is an excellent clone, and seems to be more > specific than SP1 in the work-up of metastatic carcinoma of unknown > primary site, based on the published literature. The advantage of > 6F11 is that, for those of us who use the Allred scoring system, > it's the only clone that was clinically validated by Harvey et al. > (JCO 1999) for this purpose. I agree with Rich. > > For those who use SP1, it's a very good clone as a predictive marker > in breast cancer. But again, in the setting of metastatic workup, it > is NOT recommended, as it will pick up too many primary lung cancers > and some colon cancers (personal experience). > > Hadi > > ================================ > Hadi Yaziji, M.D., Medical Director > Vitro Molecular Laboratories > President, > Ancillary Pathways > 7000 62nd Avenue, PH-C > Miami, FL 33143 > T 305-740-4440 > F. 786-513-0175 > www.vitromolecular.com > www.ancillarypath.com > > > > On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: > > > I have looked at several clones over the years and I prefer clone > 6F11. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Taylor, Jean" 4/23/2010 11:17 AM >>> > > I'm wondering which clone of ER most labs are using? > > Thanks, > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > > -- > Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en > > > > This information is confidential and intended solely for the use of > the individual or entity to whom it is addressed. If you have > received this email in error please notify the sender or our > Customer Support Center at (262) 928-2777. We have scanned this e- > mail and its attachments for malicious content. However, the > recipient should check this email and any attachments for the > presence of viruses. ProHealth Care accepts no liability for any > damage caused by any virus transmitted by this email. From Bill.Tench <@t> pph.org Mon Apr 26 12:08:08 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Apr 26 12:08:22 2010 Subject: [Histonet] histotech positions in San Diego area In-Reply-To: <20100426170203.2F2EC12BB69@mail1.pph.org> References: <20100426170203.2F2EC12BB69@mail1.pph.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02862FEB@MAIL1.pph.local> Life is uncertain, but........ We may have an opening in the future. You may send resumes directly to this email address and I will forward them on to the appropriate people. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, April 26, 2010 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 77, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: [IHCRG] ER clone 1D5 or SP1 ? (Richard Cartun) 2. Region 1 conference (Amos Brooks) 3. Re: [IHCRG] ER clone 1D5 or SP1 ? (ancillarypath@mac.com) 4. Mysterious artifact on GI biopsies H&E stain (Urim, Lyudmila) 5. paraformaldayhde (Perry, Margaret) 6. RE: Mysterious artifact on GI biopsies H&E stain (Weems, Joyce) 7. anti 8 hydroxyguanosine (Fabrice gankam) 8. RE: Mysterious artifact on GI biopsies H&E stain (Mike Pence) 9. Any Histology openings in San Diego area? (Jill Cox) 10. (no subject) (Urim, Lyudmila) 11. Re: Two antibodies from the same host with tyramide (Johnson, Teri) 12. RE: (no subject) (Kaye Ryan) 13. RE: (no subject) (Nails, Felton) 14. RE: Best books for the HTL? (Morken, Tim) 15. RE: (no subject) (Jesus Ellin) 16. RE: [IHCRG] ER clone 1D5 or SP1 ? (Van Eyck, Deb) 17. RE: [IHCRG] ER clone 1D5 or SP1 ? (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Sun, 25 Apr 2010 15:04:28 -0400 From: "Richard Cartun" Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? To: "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" , Message-ID: <4BD459FB.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en ------------------------------ Message: 2 Date: Sun, 25 Apr 2010 19:14:49 -0400 From: Amos Brooks Subject: [Histonet] Region 1 conference To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I would like to ask if anyone has any photos of the Region 1 conference to consider emailing them to me. I was going to post some on the Region 1 Conference web page and possibly put some in the Paraffin Press. If you are interested, please drop me a line. Thanks, Amos amosbrooks@gmail.com ------------------------------ Message: 3 Date: Sun, 25 Apr 2010 20:36:18 -0400 From: ancillarypath@mac.com Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? To: "ihcrg Group (E-mail)" , histonet netserver Message-ID: <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: > I have looked at several clones over the years and I prefer clone > 6F11. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" 4/23/2010 11:17 AM >>> > > I'm wondering which clone of ER most labs are using? > > Thanks, > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > > -- > Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en > ------------------------------ Message: 4 Date: Mon, 26 Apr 2010 07:23:57 -0400 From: "Urim, Lyudmila" Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain To: Message-ID: <6774152B580A6E44B38487B24A331E7C131603E0@sasquatch.cphc.local> Content-Type: text/plain; charset="us-ascii" > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. > Also on the affected GI biopsies there is often only 1 or 2 spots of > the biopsy that are affected while the rest of the biopsy looks fine. > The artifact is being reproduced on each cut; also the artifact > doesn't disappear after the affected biopsies get reprocessed. > The artifact can be described as a foggy, smudgy stain, with deformed > nucleus and not enough cell details. > All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy ------------------------------ Message: 5 Date: Mon, 26 Apr 2010 07:43:53 -0500 From: "Perry, Margaret" Subject: [Histonet] paraformaldayhde To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ------------------------------ Message: 6 Date: Mon, 26 Apr 2010 09:48:08 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Mysterious artifact on GI biopsies H&E stain To: "Urim, Lyudmila" , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164015DCCBF11@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I would check to see what medication the patient was on or if something was done during the procedure to affect the biopsies.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 07:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. > Also on the affected GI biopsies there is often only 1 or 2 spots of > the biopsy that are affected while the rest of the biopsy looks fine. > The artifact is being reproduced on each cut; also the artifact > doesn't disappear after the affected biopsies get reprocessed. > The artifact can be described as a foggy, smudgy stain, with deformed > nucleus and not enough cell details. > All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 7 Date: Mon, 26 Apr 2010 09:00:32 -0500 From: Fabrice gankam Subject: [Histonet] anti 8 hydroxyguanosine Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hey guys was wondering if any of you used the anti 8 hydroxyguanosine to detect free radical induced DNA and RNa damage. which antibody is the best. we tried the one from abdserotec and it is a disaster. the background is just horrible. any idea ? 2010/4/26 Perry, Margaret > In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of > powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 26 Apr 2010 09:04:43 -0500 From: "Mike Pence" Subject: RE: [Histonet] Mysterious artifact on GI biopsies H&E stain To: "Urim, Lyudmila" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DDF@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" It could be a "hot biopsy"? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 6:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. Also on the affected > GI biopsies there is often only 1 or 2 spots of the biopsy that are > affected while the rest of the biopsy looks fine. The artifact is > being reproduced on each cut; also the artifact doesn't disappear > after the affected biopsies get reprocessed. The artifact can be > described as a foggy, smudgy stain, with deformed nucleus and not > enough cell details. All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 26 Apr 2010 07:31:50 -0700 (PDT) From: Jill Cox Subject: [Histonet] Any Histology openings in San Diego area? To: "Histonet@Lists. Utsouthwestern. Edu" Message-ID: <519989.77668.qm@web56803.mail.re3.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Netters, I have a friend looking to relocate to San Diego area and is looking for a Histology position, she is certified HT. Any help would be greatly appreciated, thanks in advance!! Jill Jill Cox HT (ASCP) Arizona Dermatology 4232 E Cactus Rd Phoenix AZ 85032 ------------------------------ Message: 10 Date: Mon, 26 Apr 2010 11:10:08 -0400 From: "Urim, Lyudmila" Subject: [Histonet] (no subject) To: Message-ID: <6774152B580A6E44B38487B24A331E7C131603E9@sasquatch.cphc.local> Content-Type: text/plain; charset="us-ascii" Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ------------------------------ Message: 11 Date: Mon, 26 Apr 2010 10:16:26 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Two antibodies from the same host with tyramide To: "'anonwums1@gmail.com'" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Adam, Thanks for the detailed protocol. I can't explain it either but I'll throw some ideas out there and maybe it'll stimulate discussion. Hopefully Chris vanderLoos will chime in. Have you tried doing the labeling in reverse, using the VE-Cadherin first and then blocking with the goat IgG, and then doing the other antibody/tyramide protocol? I'm not sure what effect, if any, the hydrogen peroxide might have on the anti-goat fluorescent 649 label, so you could certainly do that block prior to doing the first primary antibody incubation. Also, you may have to double the concentration of the Cadherin to get it to label at the same intensity as you do in single staining. But still I might have expected to see a signal. Do the two antibodies co-express? Could there be something with the covalent binding of the HRP-tyramide complex that shelters the antigenic sites for the second antibody? That's why I wondered what might happen if you reversed your protocol. Let us know if you get it figured out and what fixed it for you. Good luck, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 12 Date: Mon, 26 Apr 2010 11:25:50 -0400 From: "Kaye Ryan" Subject: RE: [Histonet] (no subject) To: "Urim, Lyudmila" , Message-ID: Content-Type: text/plain; charset="us-ascii" I highly recommend the Milestone microwave tissue processors. I have used their microwave tissues processors in two different locations and have found them to be very reliable, user friendly, reproducible and cost effective. They also have wonderful tech support. Kaye Ryan Histology Supervisor North Florida Dermatology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 11:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 26 Apr 2010 10:49:08 -0500 From: "Nails, Felton" Subject: RE: [Histonet] (no subject) To: "'Urim, Lyudmila'" , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii It really depends on your volume to be processed and what you are planning to process. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 10:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 14 Date: Mon, 26 Apr 2010 08:54:23 -0700 From: "Morken, Tim" Subject: RE: [Histonet] Best books for the HTL? To: "Paula Sicurello" , HistoNet Message-ID: <1AAF670737F193429070841C6B2ADD4C01818AC169@EXMBMCB15.ucsfmedicalcenter. org> Content-Type: text/plain; charset=us-ascii Paula, Carson's book is probably them most relevant to basic histology these days (Sheehans is still very good for detail on technique and stains but has a lot of old material that you will rarely, if ever, see anymore). Bancroft's book is also excellent, has more detail on given stains and has more on management (QC, etc). For the HTL I also recommend the ASCP book "Quality Management in Anatomic Pathology" by Nakhleh and Fitzgibbons. Plus the usual NSH study guides. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Sunday, April 25, 2010 7:18 AM To: HistoNet Subject: [Histonet] Best books for the HTL? Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 26 Apr 2010 09:02:43 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] (no subject) To: "Urim, Lyudmila" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6277@EXCHANGECLUSTER.yumaregional.l ocal> Content-Type: text/plain; charset="us-ascii" I would look at this very carefully, especially in light of the new regulation that were just released by the CAP on ER and PR. But that is up to each individual lab Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 8:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 16 Date: Mon, 26 Apr 2010 11:29:34 -0500 From: "Van Eyck, Deb" Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? To: , "ihcrg Group (E-mail)" , "histonet netserver" Message-ID: <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> Content-Type: text/plain; charset="US-ASCII" This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 17 Date: Mon, 26 Apr 2010 10:46:09 -0600 From: "Patsy Ruegg" Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? To: , , "'ihcrg Group \(E-mail\)'" , "'histonet netserver'" Message-ID: Content-Type: text/plain; charset="us-ascii" Did I understand correctly from Dr. Hammond in Florida that the ER ASCO/CAP guidelines extended the fixation time to 72 hours? Are they changing the Her2 guidelines to match the ER? If so, has that happened yet? I have people very anxious to stop having techs work on the weekends to comply with the 48 hour fixation limits. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Monday, April 26, 2010 10:30 AM To: ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 32 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From JoelI <@t> mcclainlab.com Mon Apr 26 12:26:52 2010 From: JoelI <@t> mcclainlab.com (Joel Israel) Date: Mon Apr 26 12:20:54 2010 Subject: [Histonet] Fibrinogen Ab Message-ID: Does anyone know a fibrinogen antibody that reacts in pig? Thank you in advance. Joel R. Israel From TJJ <@t> stowers.org Mon Apr 26 14:38:16 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Apr 26 14:38:25 2010 Subject: [Histonet] Re: Two antibodies from the same host with tyramide Message-ID: Adam, I've been doing some searching and the tyramide shouldn't shelter subsequent IHC reactions, so I don't think that's it. I found evidence of co-localization of mRNA ISH signal with tyramide enhancement and IHC staining without amplification in a publication from J Histochem Cytochem. I'm actually quite surprised you are getting single staining using the goat anti-mouse antibody from R&D systems. The one I found online (AF1002) has only been tested in Elisa and western blot, and I'm not seeing any sort of antigen retrieval in your protocol. I would suspect that most endothelial markers (with the exception perhaps of Factor VIII) would require it. The fact this is actually working for you in formalin fixed paraffin embedded mouse bone should be cause for celebration. I still would be interested in knowing if reversing the protocol would have any affect on the staining, especially if you can do the Cadherin first, verify staining briefly, then continue on with the other antibody and its detection and see what happens. Best wishes, Teri Johnson From akbitting <@t> geisinger.edu Mon Apr 26 14:39:31 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Apr 26 14:39:40 2010 Subject: [Histonet] Ventana EMA antibody Message-ID: <4BD5B3B3.2B7F.00C9.0@geisinger.edu> Histofriends, Has anyone had success staining an ependymoma with Ventanas EMA antibody? My meningiomas stain nicely. I have a case that I've stained with Dakos EMA on an Autostainer, and it shows the expected spotty pattern. Anyone out there have a successful protocol? Happy Monday, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From TJJ <@t> stowers.org Mon Apr 26 15:23:48 2010 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Apr 26 15:23:55 2010 Subject: [Histonet] Re: paraformaldehyde Message-ID: Margaret, good luck getting 37 grams of powdered PFA to go into solution. Even making a 10% solution is difficult, usually requiring heat (60 degrees C) and/or sodium hydroxide to get it into solution. Once you do, it's going to want to repolymerize and you'll end up with a real mess. You'd do much better to use stock 37% formaldehyde you can buy commercially. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From MSHERWOOD <@t> PARTNERS.ORG Mon Apr 26 15:30:49 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Apr 26 15:30:21 2010 Subject: [Histonet] Re: paraformaldehyde In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24236@PHSXMB30.partners.org> I ditto Teri's suggestion. In a recent email to you, I mentioned the dangers of using powdered paraformaldehyde. I have since stopped using it. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Monday, April 26, 2010 4:24 PM To: 'margaret.perry@sdstate.edu' Cc: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: paraformaldehyde Margaret, good luck getting 37 grams of powdered PFA to go into solution. Even making a 10% solution is difficult, usually requiring heat (60 degrees C) and/or sodium hydroxide to get it into solution. Once you do, it's going to want to repolymerize and you'll end up with a real mess. You'd do much better to use stock 37% formaldehyde you can buy commercially. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Gina.Rodriguez <@t> leica-microsystems.com Mon Apr 26 16:01:14 2010 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Mon Apr 26 16:01:21 2010 Subject: [Histonet] Gina Rodriguez is out of the office. Message-ID: I will be out of the office starting 04/26/2010 and will not return until 05/03/2010. I will respond to your message when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From deb.vaneyck <@t> phci.org Mon Apr 26 17:11:55 2010 From: deb.vaneyck <@t> phci.org (Van Eyck, Deb) Date: Mon Apr 26 17:13:08 2010 Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <7EAF931905B1E440B21C11B545217E131B68C380@SKMSG02.sickkids.ca> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> <4BD459FB.7400.0077.1@harthosp.org> <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com>, <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> <7EAF931905B1E440B21C11B545217E131B68C380@SKMSG02.sickkids.ca> Message-ID: <37212590E341574B96E6796D27DF42DAB04E9B@RWDEX3.WHS.phci.org> Thanks Michael ----I saw table 10 -but on page 16 they specifically only list clone 1294 and 312 (which is whose?) -they really don't clearly say all clones for PR listed in table 10. They also only refer to these two again on the shortened ASCO color plate handout Additional information in re: clinical question 2.1. Deb ________________________________ From: Michael Ho [mailto:michael.ho@sickkids.ca] Sent: Monday, April 26, 2010 4:13 PM To: Van Eyck, Deb; ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? Hi Deb If you look at table 10 of the new guidelines you will see the ER/PR antibody clone list Michael ________________________________ From: ihcrg@googlegroups.com [ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb [deb.vaneyck@phci.org] Sent: April 26, 2010 12:29 PM To: ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From Tony_Reilly <@t> health.qld.gov.au Mon Apr 26 19:13:35 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Apr 26 19:15:28 2010 Subject: [Histonet] ER clone 1D5 or SP1 ? In-Reply-To: References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> Message-ID: <4BD6B8CE.471C.0039.0@health.qld.gov.au> Hi A large number of studies have been performed which show that 6F11 will stain a number of tumours that 1D5 does not and I know a number of labs who have moved away from 1D5 because of this. Google 1D5 and 6F11 and you will find the articles. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From histotech <@t> imagesbyhopper.com Mon Apr 26 22:26:43 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Apr 26 22:26:51 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: <37212590E341574B96E6796D27DF42DAB04E9B@RWDEX3.WHS.phci.org> Message-ID: <1BFAC59135A047A384ACE71EC8240E25@hopperPC> Hi Histonetters! We have started to have an issue with our bone marrow clots falling off the slides. We are using plus slides, making sure they drain well (just like our other slides), but when we stain them routinely, we are getting a fair amount of tissue coming off the slides. It has been suggested that it's related to our using recycled xylene. Does anyone have any experience with recycled xylene and this type of tissue fall-off? Another suggestion was that the tissues sat in xylene too long. I don't see how that could happen, under routine conditions, but is that a possibility for causing this? All thoughts will be appreciated! Michelle From hodges420 <@t> msn.com Mon Apr 26 23:18:58 2010 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Mon Apr 26 23:19:03 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: <1BFAC59135A047A384ACE71EC8240E25@hopperPC> References: <37212590E341574B96E6796D27DF42DAB04E9B@RWDEX3.WHS.phci.org>, <1BFAC59135A047A384ACE71EC8240E25@hopperPC> Message-ID: recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols > From: histotech@imagesbyhopper.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Apr 2010 23:26:43 -0400 > Subject: [Histonet] Bone Marrow Clots - falling off slides > > Hi Histonetters! > > We have started to have an issue with our bone marrow clots falling off the > slides. We are using plus slides, making sure they drain well (just like > our other slides), but when we stain them routinely, we are getting a fair > amount of tissue coming off the slides. > > It has been suggested that it's related to our using recycled xylene. Does > anyone have any experience with recycled xylene and this type of tissue > fall-off? > > Another suggestion was that the tissues sat in xylene too long. I don't see > how that could happen, under routine conditions, but is that a possibility > for causing this? > > All thoughts will be appreciated! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1 From lpaveli1 <@t> hurleymc.com Tue Apr 27 05:55:32 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Apr 27 05:55:51 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: References: <37212590E341574B96E6796D27DF42DAB04E9B@RWDEX3.WHS.phci.org> , <1BFAC59135A047A384ACE71EC8240E25@hopperPC> Message-ID: <4BD68A64.59CD.00EE.0@hurleymc.com> When I start losing tissue on the slide, I have found that it is usually a processing issue. Is the clot too thick? Try cutting them in half before processing. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> MARY T HODGES 4/27/2010 12:18 AM >>> recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols > From: histotech@imagesbyhopper.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Apr 2010 23:26:43 -0400 > Subject: [Histonet] Bone Marrow Clots - falling off slides > > Hi Histonetters! > > We have started to have an issue with our bone marrow clots falling off the > slides. We are using plus slides, making sure they drain well (just like > our other slides), but when we stain them routinely, we are getting a fair > amount of tissue coming off the slides. > > It has been suggested that it's related to our using recycled xylene. Does > anyone have any experience with recycled xylene and this type of tissue > fall-off? > > Another suggestion was that the tissues sat in xylene too long. I don't see > how that could happen, under routine conditions, but is that a possibility > for causing this? > > All thoughts will be appreciated! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Tue Apr 27 06:32:49 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Apr 27 06:33:04 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: <4BD68A64.59CD.00EE.0@hurleymc.com> Message-ID: Mary, Thanks for the suggestion. We do check the alcohols with a hydrometer and if not 100%, we will use that alcohol as the base for dilution alcohols. We check quality of the xylene by putting 10ml of water in a graduated cylinder and then add 10ml of the recycled xylene to it and shake it vigorosly. Good, clean xylene should have a perfect separation and no "mixing" with the water. Lynette, I am leaning towards the processing too, but which part?? The clot seems to be more dry than moist, so it's not under dehydrated. Fixation? It spends 3 hours in formalin on the tissue processor and whatever time it can be in formalin prior to the processor. Paraffin impregnation? About the only thing I could think of here was if the wax wasn't changed fast enough and/or we got too much carry over from the xylene. Could that cause this? Thanks for the help! Michelle -----Original Message----- From: Lynette Pavelich [mailto:lpaveli1@hurleymc.com] Sent: Tuesday, April 27, 2010 6:56 AM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; MARY HODGES Subject: RE: [Histonet] Bone Marrow Clots - falling off slides When I start losing tissue on the slide, I have found that it is usually a processing issue. Is the clot too thick? Try cutting them in half before processing. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> MARY T HODGES 4/27/2010 12:18 AM >>> recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols > From: histotech@imagesbyhopper.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Apr 2010 23:26:43 -0400 > Subject: [Histonet] Bone Marrow Clots - falling off slides > > Hi Histonetters! > > We have started to have an issue with our bone marrow clots falling off the > slides. We are using plus slides, making sure they drain well (just like > our other slides), but when we stain them routinely, we are getting a fair > amount of tissue coming off the slides. > > It has been suggested that it's related to our using recycled xylene. Does > anyone have any experience with recycled xylene and this type of tissue > fall-off? > > Another suggestion was that the tissues sat in xylene too long. I don't see > how that could happen, under routine conditions, but is that a possibility > for causing this? > > All thoughts will be appreciated! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:W L:en-US:WM_HMP:042010_1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2835 - Release Date: 04/25/10 18:31:00 From lpaveli1 <@t> hurleymc.com Tue Apr 27 07:59:07 2010 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Apr 27 07:59:27 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: References: <4BD68A64.59CD.00EE.0@hurleymc.com> Message-ID: <4BD6A75A.59CD.00EE.0@hurleymc.com> In my experience, it can be all of the above that can cause it. By the tissue not getting enough penetration by any of the solutions will alter the outcome. That is why I asked about the thickness (remember...only as thick as a nickel). Blood is also so dense, so it would really apply here. Try that. Do 1 change at a time. I've been in your shoes, it is very frustrating. Then if the thickness is good, work on something else.....starting with fixation times. >>> 4/27/2010 7:32 AM >>> Mary, Thanks for the suggestion. We do check the alcohols with a hydrometer and if not 100%, we will use that alcohol as the base for dilution alcohols. We check quality of the xylene by putting 10ml of water in a graduated cylinder and then add 10ml of the recycled xylene to it and shake it vigorosly. Good, clean xylene should have a perfect separation and no "mixing" with the water. Lynette, I am leaning towards the processing too, but which part?? The clot seems to be more dry than moist, so it's not under dehydrated. Fixation? It spends 3 hours in formalin on the tissue processor and whatever time it can be in formalin prior to the processor. Paraffin impregnation? About the only thing I could think of here was if the wax wasn't changed fast enough and/or we got too much carry over from the xylene. Could that cause this? Thanks for the help! Michelle -----Original Message----- From: Lynette Pavelich [mailto:lpaveli1@hurleymc.com] Sent: Tuesday, April 27, 2010 6:56 AM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; MARY HODGES Subject: RE: [Histonet] Bone Marrow Clots - falling off slides When I start losing tissue on the slide, I have found that it is usually a processing issue. Is the clot too thick? Try cutting them in half before processing. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> MARY T HODGES 4/27/2010 12:18 AM >>> recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols > From: histotech@imagesbyhopper.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Apr 2010 23:26:43 -0400 > Subject: [Histonet] Bone Marrow Clots - falling off slides > > Hi Histonetters! > > We have started to have an issue with our bone marrow clots falling off the > slides. We are using plus slides, making sure they drain well (just like > our other slides), but when we stain them routinely, we are getting a fair > amount of tissue coming off the slides. > > It has been suggested that it's related to our using recycled xylene. Does > anyone have any experience with recycled xylene and this type of tissue > fall-off? > > Another suggestion was that the tissues sat in xylene too long. I don't see > how that could happen, under routine conditions, but is that a possibility > for causing this? > > All thoughts will be appreciated! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:W L:en-US:WM_HMP:042010_1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2835 - Release Date: 04/25/10 18:31:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSimoskevitz <@t> osteotech.com Tue Apr 27 08:09:43 2010 From: RSimoskevitz <@t> osteotech.com (Ricki Simoskevitz) Date: Tue Apr 27 08:09:49 2010 Subject: [Histonet] cell types Message-ID: Hello Histonetters, Can anyone out there help us. We need to be able to differentiate between mesenchymal cells, fibroblasts and macrophages. We currently embed in GMA. I am thinking that we would have to go into paraffin and do immunohistochemistry to label each cell type. Is this something that can be done and if so does anyone have a procedure or at least a place I can go to find out more information on how to go about doing this. Thanks for your help. Ricki Simoskevitz rsimoskevitz@osteotech.com ________________________________ CONFIDENTIAL COMMUNICATION: E-mails from Osteotech generally contain information that is confidential, privileged, and/or protected as work product or by other legal rules, and are for the sole use of the intended recipient. Any use, copying, or distribution of this e-mail by an unintended recipient is prohibited, and may be a violation of law. If you believe that you received this e-mail in error, please do not read this e-mail or any attached items, and instead please delete the e-mail and all attachments. From histotech <@t> imagesbyhopper.com Tue Apr 27 08:20:29 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Apr 27 08:20:41 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: <4BD6A75A.59CD.00EE.0@hurleymc.com> References: <4BD68A64.59CD.00EE.0@hurleymc.com> <4BD6A75A.59CD.00EE.0@hurleymc.com> Message-ID: Thanks for all the input. I will check the collection method. B-plus is the fixative we normally use but I am not convinced that this clot ended up in it! Other tissues are not being dramatically affected and not even all the bone marrows! We do soak the blocks too. This is very frustrating. :o( Michelle On Apr 27, 2010, at 7:59 AM, "Lynette Pavelich" wrote: > In my experience, it can be all of the above that can cause it. By > the > tissue not getting enough penetration by any of the solutions will > alter > the outcome. That is why I asked about the thickness (remember...only > as thick as a nickel). Blood is also so dense, so it would really > apply > here. Try that. Do 1 change at a time. I've been in your shoes, > it is > very frustrating. Then if the thickness is good, work on something > else.....starting with fixation times. > > >>>> 4/27/2010 7:32 AM >>> > Mary, > Thanks for the suggestion. We do check the alcohols with a hydrometer > and > if not 100%, we will use that alcohol as the base for dilution > alcohols. We > check quality of the xylene by putting 10ml of water in a graduated > cylinder > and then add 10ml of the recycled xylene to it and shake it vigorosly. > Good, clean xylene should have a perfect separation and no "mixing" > with > the water. > > Lynette, > I am leaning towards the processing too, but which part?? The clot > seems to > be more dry than moist, so it's not under dehydrated. Fixation? It > spends 3 > hours in formalin on the tissue processor and whatever time it can be > in > formalin prior to the processor. Paraffin impregnation? About the > only > thing I could think of here was if the wax wasn't changed fast enough > and/or > we got too much carry over from the xylene. Could that cause this? > > Thanks for the help! > > Michelle > > > > -----Original Message----- > From: Lynette Pavelich [mailto:lpaveli1@hurleymc.com] > Sent: Tuesday, April 27, 2010 6:56 AM > To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; > MARY > HODGES > Subject: RE: [Histonet] Bone Marrow Clots - falling off slides > > > When I start losing tissue on the slide, I have found that it is > usually a > processing issue. Is the clot too thick? Try cutting them in half > before > processing. > > Hope this helped, > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > MSH Competency Coordinator > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > email: Lpaveli1@hurleymc.com > ph: 810-257-9948 > Lab: 810-257-9138 > fax: 810-762-7082 > > >>>> MARY T HODGES 4/27/2010 12:18 AM >>> > > recycled anything is not back to it original state always use a hydo > meter > and add new to back product of a better quality you will find this > also > with > alcohols > >> From: histotech@imagesbyhopper.com >> To: histonet@lists.utsouthwestern.edu >> Date: Mon, 26 Apr 2010 23:26:43 -0400 >> Subject: [Histonet] Bone Marrow Clots - falling off slides >> >> Hi Histonetters! >> >> We have started to have an issue with our bone marrow clots falling > off the >> slides. We are using plus slides, making sure they drain well (just > like >> our other slides), but when we stain them routinely, we are getting > a > fair >> amount of tissue coming off the slides. >> >> It has been suggested that it's related to our using recycled > xylene. > Does >> anyone have any experience with recycled xylene and this type of > tissue >> fall-off? >> >> Another suggestion was that the tissues sat in xylene too long. I > don't see >> how that could happen, under routine conditions, but is that a > possibility >> for causing this? >> >> All thoughts will be appreciated! >> >> Michelle >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail has tools for the New Busy. Search, chat and e-mail from your > inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:W > > L:en-US:WM_HMP:042010_1_______________________________________________ > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.437 / Virus Database: 271.1.1/2835 - Release Date: > 04/25/10 > 18:31:00 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From NSEARCY <@t> swmail.sw.org Tue Apr 27 08:41:46 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Apr 27 08:41:53 2010 Subject: [Histonet] "Better Communication with Histotechnicians" Message-ID: <4BD6A34A.5D38.00EF.0@swmail.sw.org> I have a request from a physician that is presenting a talk to Mohs techs regarding developing better communication skills ---anyone have any good references for him? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From gu.lang <@t> gmx.at Tue Apr 27 08:49:55 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 27 08:50:05 2010 Subject: [Histonet] searching the right PI3K antibody Message-ID: Hi all! For investigation lung-cancer for targeted therapy, which PI3K antibody should be used or which isoform/part of PI3K has to be detected? It's for human FFPE tissue. We stain on Benchmark XT. Thanks for any help. Gudrun Lang Histolab Akh Linz, Austria From relia1 <@t> earthlink.net Tue Apr 27 08:55:05 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 27 08:55:10 2010 Subject: [Histonet] RELIA Histology Job Alert 4/27/2010 Message-ID: Hi Histonetters! I hope everyone is having a great day. I would like to take a minute and tell you about some new positions I am working on. All of these are permanent full time opportunities and my clients offer excellent compensation, benefits and relocation assistance. Histology Management: Night Shift Supervisor - Just outside of Boston, MA Histology Manager - Long Island, NY no NYS license required! Histology Positions: Night Shift Histotech - Just outside of Boston, MA Immunohistochemistry Specialist - Atlanta, GA Histotechnician/Histotechnologist - Orang/Rockland County NY If you or anyone you know might be interested in hearing about any of these positions or would like to initiate a personalized job search in a specific area please contact me. I offer over 25 years of experience as a recruiter helping people attain their career objectives. I can be reached at 866-607-3542 or relia1@earthlink.net and am available all day every day and after hours too. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From gu.lang <@t> gmx.at Tue Apr 27 08:57:06 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 27 08:57:12 2010 Subject: [Histonet] active ingredient in formaldehyde solution? Message-ID: <0EE787683BA04602BFDFF0CD9B3CB4BF@dielangs.at> Hi, what is the common opinion about the active ingredient in formaldehyde solution? Is it formaldehyde or methylenglycol (di-hydroxy-methan), that fixes the tissue? Or both? In solution most of the formaldehyde is hydrated and forms methylenglycol, only a small part remains as formaldehyde. Gudrun From NSEARCY <@t> swmail.sw.org Tue Apr 27 08:59:32 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Apr 27 08:59:39 2010 Subject: [Histonet] Skip Brown Message-ID: <4BD6A773.5D38.00EF.0@swmail.sw.org> Can anyone get me in contact with Skip? He maybe able to help me with this communication topic. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From Sandra.Etheridge <@t> gov.bc.ca Tue Apr 27 09:34:39 2010 From: Sandra.Etheridge <@t> gov.bc.ca (Etheridge, Sandra AL:EX) Date: Tue Apr 27 09:34:52 2010 Subject: [Histonet] Koster's Stain Message-ID: Hello everyone, Just wondering if anyone has the staining protocol for Koster's Stain. Apparently is stains Brucella organisms but I can't find anything in my reference texts or online. Any information is, as always, appreciated. Sandra From POWELL_SA <@t> mercer.edu Tue Apr 27 09:42:30 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Apr 27 09:42:37 2010 Subject: [Histonet] Bone Marrow Clots - falling off slides In-Reply-To: References: <4BD68A64.59CD.00EE.0@hurleymc.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE2268C0C87BD@MERCERMAIL.MercerU.local> I cut the autopsies for the ME/FBI here and have a lot of bloody tissues I lovingly call "blood bricks". I was using charged slides but these tissue would wash really badly. I started using regular slides and StayOn from Surgipath and have had great success with keeping these bloody tissues on the slides. I drain the water well, heat the slides up just to the point of melting the paraffin and dry them overnight in the slide dryer to make sure, but have also dried them for 45 minutes and they have stayed on. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Tuesday, April 27, 2010 7:33 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone Marrow Clots - falling off slides Mary, Thanks for the suggestion. We do check the alcohols with a hydrometer and if not 100%, we will use that alcohol as the base for dilution alcohols. We check quality of the xylene by putting 10ml of water in a graduated cylinder and then add 10ml of the recycled xylene to it and shake it vigorosly. Good, clean xylene should have a perfect separation and no "mixing" with the water. Lynette, I am leaning towards the processing too, but which part?? The clot seems to be more dry than moist, so it's not under dehydrated. Fixation? It spends 3 hours in formalin on the tissue processor and whatever time it can be in formalin prior to the processor. Paraffin impregnation? About the only thing I could think of here was if the wax wasn't changed fast enough and/or we got too much carry over from the xylene. Could that cause this? Thanks for the help! Michelle -----Original Message----- From: Lynette Pavelich [mailto:lpaveli1@hurleymc.com] Sent: Tuesday, April 27, 2010 6:56 AM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; MARY HODGES Subject: RE: [Histonet] Bone Marrow Clots - falling off slides When I start losing tissue on the slide, I have found that it is usually a processing issue. Is the clot too thick? Try cutting them in half before processing. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> MARY T HODGES 4/27/2010 12:18 AM >>> recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols > From: histotech@imagesbyhopper.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Apr 2010 23:26:43 -0400 > Subject: [Histonet] Bone Marrow Clots - falling off slides > > Hi Histonetters! > > We have started to have an issue with our bone marrow clots falling off the > slides. We are using plus slides, making sure they drain well (just like > our other slides), but when we stain them routinely, we are getting a fair > amount of tissue coming off the slides. > > It has been suggested that it's related to our using recycled xylene. Does > anyone have any experience with recycled xylene and this type of tissue > fall-off? > > Another suggestion was that the tissues sat in xylene too long. I don't see > how that could happen, under routine conditions, but is that a possibility > for causing this? > > All thoughts will be appreciated! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:W L:en-US:WM_HMP:042010_1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2835 - Release Date: 04/25/10 18:31:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Tue Apr 27 10:20:31 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Apr 27 10:20:36 2010 Subject: [Histonet] ?New CAP guidelines affect CLIA '88 Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C6D@wlmmsx01.nemours.org> Histonetters familiar with CAP and CLIA compliance issue...I am requesting a clarification: Will the new CAP guidelines related to "processing" and "grossing" affect CLIA regulations? I understand they will certainly affect CAP labs....but do they impact CLIA labs as well? Is there any grandfathering for experienced technicians who have been doing this for years (I have 2 techs who "gross" muscle only... on tissue for enzyme histochemistry - prior to snap-freezing)......? How does this, if it does, impact technicians recognized under CLIA as "individuals who perform high complexity testing..." I know there will be change for CAP...will CLIA be following? From deliadfam <@t> yahoo.com Tue Apr 27 10:33:40 2010 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Tue Apr 27 10:33:45 2010 Subject: [Histonet] ?New CAP guidelines affect CLIA '88 In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C6D@wlmmsx01.nemours.org> Message-ID: <890599.56644.qm@web63107.mail.re1.yahoo.com> I too am curious about this issue. Any info would be oh so greatly appreciated. ? Thanks --- On Tue, 4/27/10, Barone, Carol wrote: From: Barone, Carol Subject: [Histonet] ?New CAP guidelines affect CLIA '88 To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 27, 2010, 8:20 AM Histonetters familiar with CAP and CLIA compliance issue...I am requesting a clarification: Will the new CAP guidelines related to "processing" and "grossing" affect CLIA regulations? I understand they will certainly affect CAP labs....but do they impact CLIA labs as well? Is there any grandfathering for experienced technicians who have been doing this for years (I have 2 techs who "gross" muscle only... on tissue for enzyme histochemistry -? prior to snap-freezing)......? How does this, if it does, impact technicians recognized under CLIA as "individuals who perform high complexity testing..." I know there will be? change for CAP...will CLIA be following? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jason.madore <@t> gmail.com Tue Apr 27 11:27:04 2010 From: jason.madore <@t> gmail.com (Jason Madore) Date: Tue Apr 27 11:27:11 2010 Subject: [Histonet] autostainer suggestions? Message-ID: Hello, Looking to purchase an autostainer for our research lab. We stain most TMAs with chromagen but would like to also be able to do FISH and multi-label fluorescence. Can anyone suggest a good system? Thanks in advance, Jason --- Jason Madore, MSc Research Assistant University of Montreal Hospital Centre Research Centre (CRCHUM) 2099 rue Alexandre DeS?ve. Room Y-4624 Montr?al, Qu?bec, Canada, H2L 2W5 514-890-8000 p24647 From Timothy.Morken <@t> ucsfmedctr.org Tue Apr 27 11:52:10 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Tue Apr 27 11:52:20 2010 Subject: [Histonet] ?New CAP guidelines affect CLIA '88 In-Reply-To: <890599.56644.qm@web63107.mail.re1.yahoo.com> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A7C6D@wlmmsx01.nemours.org> <890599.56644.qm@web63107.mail.re1.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C01819D06D6@EXMBMCB15.ucsfmedicalcenter.org> Carol wrote: "Will the new CAP guidelines related to "processing" and "grossing" affect CLIA regulations? " What CAP requires does not "affect CLIA regulations;" it is the other way around. It may be that CAP is simply fine tuning its requirements to better reflect the CLIA regulations. CAP is a deemed agent by CMS (Centers for Medicare and Medicaid) the Federal agency that implements the CLIA rules. The Joint Commission is another deemed agent. As such they apply and are chosen to enforce the CLIA rules. As deemed agents CAP and JC must enforce the rules, but have some leeway in how they go about it and may emphasize different aspects.+ Certainly CMS has to approve how CAP/JC does that. But what CAP does will not necessarily affect how JC goes about the same task. My experience with all three (CAP, JC and federal CLIA inspections) are that CAP emphasizes the technical aspects and secondarily the QA aspects while JC and CLIA emphasize the QA aspects, especially over the entire organization (for instance JC does "tracers" in which they pull all the information about a given patient and follow every path to the ultimate QA documents of every test on that patient - down to who stained the slides and are their competency documents in order). Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA Sent: Tuesday, April 27, 2010 8:34 AM To: histonet@lists.utsouthwestern.edu; CarolBarone Subject: Re: [Histonet] ?New CAP guidelines affect CLIA '88 I too am curious about this issue. Any info would be oh so greatly appreciated. ? Thanks --- On Tue, 4/27/10, Barone, Carol wrote: From: Barone, Carol Subject: [Histonet] ?New CAP guidelines affect CLIA '88 To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 27, 2010, 8:20 AM Histonetters familiar with CAP and CLIA compliance issue...I am requesting a clarification: Will the new CAP guidelines related to "processing" and "grossing" affect CLIA regulations? I understand they will certainly affect CAP labs....but do they impact CLIA labs as well? Is there any grandfathering for experienced technicians who have been doing this for years (I have 2 techs who "gross" muscle only... on tissue for enzyme histochemistry -? prior to snap-freezing)......? How does this, if it does, impact technicians recognized under CLIA as "individuals who perform high complexity testing..." I know there will be? change for CAP...will CLIA be following? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Tue Apr 27 12:16:03 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Apr 27 12:16:14 2010 Subject: [Histonet] Cap guidelines and Clia In-Reply-To: <20100427170040.2797212BFBC@mail1.pph.org> References: <20100427170040.2797212BFBC@mail1.pph.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863006@MAIL1.pph.local> It is important to understand that CLIA is the driving force for most of the various regulations promulgated by CAP, and this is true of the issue regarding the requirements for Gross room assistants. The CAP simply tries to interpret the morass of regulatory garbage into understandable standards, which must be approved by CLIA. There are some CAP standards that have grown out of "good laboratory practices" and not specific CLIA regulations, but this is not one of them. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From NSEARCY <@t> swmail.sw.org Tue Apr 27 13:36:32 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Apr 27 13:36:41 2010 Subject: [Histonet] Powerpath chat Message-ID: <4BD6E860.5D38.00EF.0@swmail.sw.org> Can a powerpath user send me the link for users chats? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From zodiac29 <@t> comcast.net Tue Apr 27 14:17:27 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Tue Apr 27 14:17:30 2010 Subject: [Histonet] toe nails falling off slides Message-ID: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny From ekronenberger <@t> sbcglobal.net Tue Apr 27 14:36:10 2010 From: ekronenberger <@t> sbcglobal.net (Elizabeth Kronenberger) Date: Tue Apr 27 14:34:00 2010 Subject: [Histonet] toe nails falling off slides In-Reply-To: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <0DEA785AA28942EAB271D8F800C11833@dlne.local> Using a plus slide, dip the slide into the water bath then add a small drop of Elmer's glue at one end and spread the glue with your finger along the length of the slide so that there is a thin film of glue. While the glue is still wet, add your sections and they should stay on through staining. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Tuesday, April 27, 2010 3:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dholmes <@t> anatomy.umsmed.edu Tue Apr 27 14:57:20 2010 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Tue Apr 27 14:57:38 2010 Subject: [Histonet] Fwd: New email address !!! Message-ID: <4BD6FB50020000820004FD39@GWIA1.umsmed.edu> >>> Dianne Holmes 4/27/2010 2:46 PM >>> >>> Dianne Holmes 4/27/2010 2:45 PM >>> UMC has changed carriers and now I have a new email address. It is very close to the old one so make sure you note the difference. Please REPLY to this mailing so I will know it is working. www.DHolmes@umc.edu Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From MSHERWOOD <@t> PARTNERS.ORG Tue Apr 27 14:59:20 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Apr 27 14:59:16 2010 Subject: [Histonet] Special Stains Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2424E@PHSXMB30.partners.org> We are a research lab and occasionally do a few special stains (Masson Trichrome, PAS, Picro-Sirius Red, Safranin O). We usually purchase our stains ready made (or at least the components). However, some of these stains are very expensive, namely the reagents for Masson Trichrome. We just started preparing our Safranin O from powder form. If you purchase ready-made stains, what vendors can you recommend? I have only dealt with the widely known ones (i.e. Poly Scientific, etc.) Or do you make up your stains from powders? Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From dholmes <@t> anatomy.umsmed.edu Tue Apr 27 15:29:14 2010 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Tue Apr 27 15:29:35 2010 Subject: [Histonet] New email address Message-ID: <4BD702CA020000820004FDB3@GWIA1.umsmed.edu> Please change my email address to www.DHolmes@umc.edu Thank you Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From Bill.Tench <@t> pph.org Tue Apr 27 15:29:45 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Apr 27 15:29:52 2010 Subject: [Histonet] Patient ID on cassettes In-Reply-To: <20100425170044.212AD12A7E7@mail1.pph.org> References: <20100425170044.212AD12A7E7@mail1.pph.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD0286300F@MAIL1.pph.local> A statement was made in a previous posting indicating CAP requirements for 2 unique identifiers throughout the entire analytic process: "To conform to CAP and state regulations that require two unique patient identifiers on a specimen at all analytical steps." The CAP standards GEN 40491, ANP 11460, and ANP12092 specifically indicate two unique identifiers on the PRIMARY specimen container, not on every container throughout the process, and there are notes explaining the requirements. There may be variations in State laws, but these are the most up to date CAP standards. There is no need to go through a lot of contortions adding additional information to cassettes. (however, at least the ones we use also have a side panel which can be written on) Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, April 25, 2010 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 77, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Patient ID on cassettes (Jeffrey Silverman) 2. Re: Gram stain (Maxim Peshkov) 3. Best books for the HTL? (Paula Sicurello) ---------------------------------------------------------------------- Message: 1 Date: Sat, 24 Apr 2010 10:28:55 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] Patient ID on cassettes To: Joyce.Cline@wchsys.org Cc: histonet@lists.utsouthwestern.edu Message-ID: <690158.71961.qm@web111108.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 To conform to CAP and state regulations that require two unique patient identifiers on a specimen at all analytical steps, and to cope with a persnickety cassette labeller that is down more than up, we have taken to writing the patient's first and last initials in the upper left corner of the writing surface of the cassette, above the S10- xxxx. Our pencil sharpeners are running non -stop? but it works. Only problem is when a block is sent out for IHC the returned slides are labelled with the initials as part of the reference slides accession numbers and my boss doesn't care for that. Originally we dropped the S in S10- and replaced with the initials to get a little more room, now? we added back the S and placed initials above S10-. Patient safety dictates that a prossector verify a match among? the labelled cassette and the labelled specimen containers and against the requisition and the number dictated into the gross computer/transcriber. Case by case, on every case. There is no substitute. As for placing the wrong cassettes on the wrong specimen containers, that seems to me to be pure carelessness and a disciplinary issue if it continues. When labelling, matching the initials that the microtomist writes on the slide with the patient's name printed on the slide labels provides a final? ID double check if the numbers on the slide get transcribed incorrectly. The initials thing takes minimal effort, but you need to be able to write small LOL. Jeff Silverman. ------------------------------ Message: 2 Date: Sat, 24 Apr 2010 23:08:48 +0400 From: Maxim Peshkov Subject: Re: [Histonet] Gram stain To: "Perry, Margaret" Cc: histonet@lists.utsouthwestern.edu Message-ID: <573298402.20100424230848@mail.ru> Content-Type: text/plain; charset=windows-1251 Margaret: We uses 1% aqueous neutral red as red counterstain with very good results. Do not forget rinse slides at 10-15 secs in DW with 1-2 drop of glacial acetic acid before neutral red and after this, because this dye have red color at pH < 6. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Fri, 23 Apr 2010 16:25:30 -0500 > From: "Perry, Margaret" > Subject: [Histonet] Gram stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Now that I'm done with my rant I have a real > question. We are trying to do a gram stain on fish > and the safranine O is staining everything red. What > other stain would you use? I usually have time to > look at the books but unfortunately it's almost > quiting time and the slides need to be done by noon Monday. Thanks > Margaret Perry mailto:Maxim_71@mail.ru ------------------------------ Message: 3 Date: Sun, 25 Apr 2010 10:18:13 -0400 From: Paula Sicurello Subject: [Histonet] Best books for the HTL? To: HistoNet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 31 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From vavalos <@t> allergydermatology.com Tue Apr 27 15:33:49 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Apr 27 15:33:52 2010 Subject: [Histonet] H&E Stain Message-ID: <000801cae648$f1fb1c00$d5f15400$@com> I stain derm tissue. We do H&E only using a linear stainer. Two of my docs seem to say that stain is very eosinophilic & smudgy. Other Two say not enough Eosin. Of course!! Followed is my stain protocol. This week I have eliminated the Define and slides seem to not look as eosinophilic and I have not heard any negative remarks but it may be to soon. Any one have any suggestions to change??? Sub X SubX Sub X !00% Alc 100% Alc 100& Alc 95% Alc 95% Alc Water Hemat Hemat Hemat Water Water Define Water Blue Water 95% Alc Eosin 95% Alc 100% Alc 100% Alc SubX Sub X V.Avalos ADS, INC Fax:602-277-2134 From Maria.Katleba <@t> stjoe.org Tue Apr 27 17:22:15 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Apr 27 17:22:45 2010 Subject: [Histonet] toe nails falling off slides In-Reply-To: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide.... (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From talulahgosh <@t> gmail.com Tue Apr 27 17:45:22 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Apr 27 17:45:32 2010 Subject: [Histonet] toe nails falling off slides In-Reply-To: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: Can I ask, what exactly are you looking at toenails for? Is it a pathology thing or....? That sounds so neat. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea On Tue, Apr 27, 2010 at 3:17 PM, wrote: > To all, > > > I was wondering if anyone out there had any tips on how to keep toenail > sections from falling off slides. We mount them on positive slides, air dry > for about an hour but they still fall off. Any insight on the subject would > be greatly appreciated. > > > Thanks, > > > Jenny > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Tue Apr 27 19:12:03 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Apr 27 19:12:06 2010 Subject: [Histonet] Re: Bone Marrow Clots - falling off slides Message-ID: When I bring the bone marrow clot back to the lab from the procedure, I take it out of the bottle and cut it into very thin slices and drop the slices back into the fixative. Never have any trouble with the sections. Gudrun Lang - habe 'ne Litre-Flasche Gr?ner Veltliner - Bio-Weingut Hofer - Auerthal - Nieder?sterreich 2009 - gestern hier in Knoxville gekauft - mit Waldmeister aus meinem Garten wird's mein Maiwein f?r 2010 sein - the liquor store clerk gleefully pointed out the litre volume and the pop-bottle cap as evidence of a truly cheap and tacky tipple, highly suitable for May wine - die Flasche liegt heut' Abend im K?hlschrank. Bob Richmond Samurai-Patholog Knoxville, Tennessee USA From gankam <@t> googlemail.com Tue Apr 27 20:56:16 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Tue Apr 27 20:56:32 2010 Subject: [Histonet] anti 8 oxoguanine, 8 hydroxyguanine In-Reply-To: References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: Dear histonetters Will like to use the anti 8 hydroxyguanine (staining the oxidised DNA and RNA to asses for free radical damage to nucleic acids)for IHC but tried the antibody from abdserotec with dissapointing results. We tried it on liver slides but LOT of background. Could any one advise on other antibody or other protocol ? Thanks y'all Dr Fabrice GANKAM Intern From jshea121 <@t> roadrunner.com Tue Apr 27 21:37:53 2010 From: jshea121 <@t> roadrunner.com (Shea's) Date: Tue Apr 27 21:37:59 2010 Subject: [Histonet] Bone Marrow Section falling off slide Message-ID: I have to agree with Lynette. Good solution as well ...change only one thing at a time. We occasionally have the same problem and the BM clots are the only tissues in the entire batch to fall off (It is usually the blood in the center of the section, the rim stays attached, which is the clue that it is not well processed). This usually happens when the pathologist submits a thick clot in a cassette. This is what we find helps when it does occur: Trim, then soak in ice water bath for about 10 mins. Cut one ribbon at 4 microns, pick up sections on + charged slides (If you cut another ribbon before soaking, these will be the sections that will tend to fall off). Trim to desired level and soak again, repeat above. You are right, it is important to drain slides well. In addition, we place slides in front of a fan for 10 mins before placing them in the 65-70 degree oven for an additional 20 mins. Cool slides and stain. (We find that the soaking really helps with sections staying on the slide). Good Luck From DKBoyd <@t> chs.net Wed Apr 28 06:53:05 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Apr 28 06:53:20 2010 Subject: [Histonet] Patient ID on cassettes In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD0286300F@MAIL1.pph.local> Message-ID: I agree with you Bill. Although we are no longer CAP inspected, we just finished up a Joint Commission inspection. My inspector was a lovely lady whom we learned a lot from as a whole in the lab. She also stated that it is the primary container that must have two identifiers. Just as a FYI she also stated that FS slides need to have the name and birth date of the patient if you don't log in the specimen until after the frozen has been completed. We are putting the last name on the side of the cassette, but have been doing this for years for our own protection. Rarely will you get the number and name incorrect. Sometimes at the end of the day there aren't any surgical specimens to buffer the endos, so this has helped us a great deal. Just my thoughts. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Tench, Bill" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2010 04:30 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Patient ID on cassettes A statement was made in a previous posting indicating CAP requirements for 2 unique identifiers throughout the entire analytic process: "To conform to CAP and state regulations that require two unique patient identifiers on a specimen at all analytical steps." The CAP standards GEN 40491, ANP 11460, and ANP12092 specifically indicate two unique identifiers on the PRIMARY specimen container, not on every container throughout the process, and there are notes explaining the requirements. There may be variations in State laws, but these are the most up to date CAP standards. There is no need to go through a lot of contortions adding additional information to cassettes. (however, at least the ones we use also have a side panel which can be written on) Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, April 25, 2010 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 77, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Patient ID on cassettes (Jeffrey Silverman) 2. Re: Gram stain (Maxim Peshkov) 3. Best books for the HTL? (Paula Sicurello) ---------------------------------------------------------------------- Message: 1 Date: Sat, 24 Apr 2010 10:28:55 -0700 (PDT) From: Jeffrey Silverman Subject: [Histonet] Patient ID on cassettes To: Joyce.Cline@wchsys.org Cc: histonet@lists.utsouthwestern.edu Message-ID: <690158.71961.qm@web111108.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 To conform to CAP and state regulations that require two unique patient identifiers on a specimen at all analytical steps, and to cope with a persnickety cassette labeller that is down more than up, we have taken to writing the patient's first and last initials in the upper left corner of the writing surface of the cassette, above the S10- xxxx. Our pencil sharpeners are running non -stop but it works. Only problem is when a block is sent out for IHC the returned slides are labelled with the initials as part of the reference slides accession numbers and my boss doesn't care for that. Originally we dropped the S in S10- and replaced with the initials to get a little more room, now we added back the S and placed initials above S10-. Patient safety dictates that a prossector verify a match among the labelled cassette and the labelled specimen containers and against the requisition and the number dictated into the gross computer/transcriber. Case by case, on every case. There is no substitute. As for placing the wrong cassettes on the wrong specimen containers, that seems to me to be pure carelessness and a disciplinary issue if it continues. When labelling, matching the initials that the microtomist writes on the slide with the patient's name printed on the slide labels provides a final ID double check if the numbers on the slide get transcribed incorrectly. The initials thing takes minimal effort, but you need to be able to write small LOL. Jeff Silverman. ------------------------------ Message: 2 Date: Sat, 24 Apr 2010 23:08:48 +0400 From: Maxim Peshkov Subject: Re: [Histonet] Gram stain To: "Perry, Margaret" Cc: histonet@lists.utsouthwestern.edu Message-ID: <573298402.20100424230848@mail.ru> Content-Type: text/plain; charset=windows-1251 Margaret: We uses 1% aqueous neutral red as red counterstain with very good results. Do not forget rinse slides at 10-15 secs in DW with 1-2 drop of glacial acetic acid before neutral red and after this, because this dye have red color at pH < 6. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Fri, 23 Apr 2010 16:25:30 -0500 > From: "Perry, Margaret" > Subject: [Histonet] Gram stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Now that I'm done with my rant I have a real > question. We are trying to do a gram stain on fish > and the safranine O is staining everything red. What > other stain would you use? I usually have time to > look at the books but unfortunately it's almost > quiting time and the slides need to be done by noon Monday. Thanks > Margaret Perry mailto:Maxim_71@mail.ru ------------------------------ Message: 3 Date: Sun, 25 Apr 2010 10:18:13 -0400 From: Paula Sicurello Subject: [Histonet] Best books for the HTL? To: HistoNet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 31 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. 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From Andrew.Prior <@t> Smith-Nephew.com Wed Apr 28 07:12:54 2010 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Wed Apr 28 07:13:05 2010 Subject: [Histonet] LR white sections Message-ID: <6C18ADDF244BF8439412C063019CFFEC0D71047E@EHS021.wound.san> Fellow histonetters, I'm looking for advice for cutting bone samples embedded in LR white resin on a microtome. We have plenty of experience cutting decalcified bone in wax and with sawing/grinding sections, but little experience in cutting thin sections of resin embedded bone. We will be using a Leica rotary microtome and tungsten carbide blades, aiming to cut at 5?m thickness. I am looking for advice on infiltration times, embedding, sectioning and staining. Any and all advice gratefully received. Many thanks Andrew Andrew Prior Histologist Smith &Nephew Research Centre York YO10 5DF UK Andrew.Prior@smith-nephew.com Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From bakevictoria <@t> gmail.com Wed Apr 28 08:11:31 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Apr 28 08:11:35 2010 Subject: [Histonet] anti 8 oxoguanine, 8 hydroxyguanine In-Reply-To: References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: Dr. Fabrice Have you looked at the Trevigen website? They have different tests that are relevent to the work you are looking at. Vikki Baker On Tue, Apr 27, 2010 at 9:56 PM, Fabrice GANKAM wrote: > > Dear histonetters > Will like to use the anti 8 hydroxyguanine (staining the oxidised DNA and > RNA to asses for free radical damage to nucleic acids)for IHC but tried the > antibody from abdserotec with dissapointing results. > We tried it on liver slides but LOT of background. > Could any one advise on other antibody or other protocol ? > Thanks y'all > > Dr Fabrice GANKAM > Intern > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RCazares <@t> schosp.org Wed Apr 28 08:58:58 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Apr 28 08:59:03 2010 Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject In-Reply-To: References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <572D1F45B44B3D4096D554B4CB40639C031FCEC742@EXCHCCRMB.schosp.org> Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to processing. My techs say they cut like butter! Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, April 27, 2010 5:22 PM To: zodiac29@comcast.net; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide.... (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From jackdodo <@t> msn.com Wed Apr 28 09:21:45 2010 From: jackdodo <@t> msn.com (Jack Dodo) Date: Wed Apr 28 09:22:59 2010 Subject: [Histonet] Charged Slides for IHC on Ventana In-Reply-To: <4BCEFC2E020000F400057587@GWIA2.umm.edu> References: ,<4BCEFC2E020000F400057587@GWIA2.umm.edu> Message-ID: We have been using a remarkably priced plus slide from Epic with GREAT success. Call Epic Scientific at 888-795-8300, the price and performance will shock you. Good Luck. > Date: Wed, 21 Apr 2010 13:22:54 -0400 > From: WBENTON@umm.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Charged Slides for IHC on Ventana > > Message: 2 > Date: Tue, 20 Apr 2010 10:27:57 -0700 > From: srishan@mail.holyname.org > Subject: [Histonet] (no subject) > To: histonet-bounces@lists.utsouthwestern.edu, > > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Hi All, > > We are using Ventana XT for our immunostaining. We tried using "Plus" > slides made by many vendors. The current vendor's pricing is way too > high! With other vendors slides we have been having problems. Please > let me know where to get some plus slides which are reasonable. I know > the recommended slides come from erie but I am not quite sure about > vendors who carry it and how much they charge. > > Thanks > > Nirmala Srishan > Histology > Holy Name Medical Center. > > Nirmala, > > We use Fisher's charged slides 12-550-15, which I'm sure are made by Erie. Pricing will depend upon your contract with a particular vendor. SurgiPath aka Leica has charged slides that work well also. If you want the etched Control Box Slides we purchase from Cardinal. > Hope this helps! > > > Walter Benton, HT(ASCP)QIHC > Histology Supervisor > University of Maryland Medical Center > Anatomic Pathology > 22 S. Greene St > Room NBW65 > Baltimore MD 21201 > (Direct) 410-328-0930 > (Lab) 410-328-5524 > (Fax) 410-328-5508 > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4 From histotech <@t> imagesbyhopper.com Wed Apr 28 09:48:21 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Apr 28 09:48:32 2010 Subject: [Histonet] toe nails falling off slides - Email found in subject In-Reply-To: <572D1F45B44B3D4096D554B4CB40639C031FCEC742@EXCHCCRMB.schosp.org> References: <1307712929.19152141272395847662.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> <572D1F45B44B3D4096D554B4CB40639C031FCEC742@EXCHCCRMB.schosp.org> Message-ID: We have very good success in facing the block and soaking it on the water bath for 30-60 seconds and then allowing it to cool down on a very wet ice tray. Ours cut like butter too! :o) On Apr 28, 2010, at 8:58 AM, "Cazares, Ruth" wrote: > Or you could soak them in 10% sodium hydroxide for 20-30 minutes > prior to processing. My techs say they cut like butter! > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > 5145 North California Ave > Chicago, IL 60625 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba > Sent: Tuesday, April 27, 2010 5:22 PM > To: zodiac29@comcast.net; histonet@lists.utsouthwestern.edu > Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - > Email found in subject > > HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on > slide! > > (1) Cut the slides and place on Plus(+) slide.... > (2) shake off excess water > (3) place slide immediately in plastic Coplin jar with 1-3 drops of > formalin at bottom. > (4) place in hot oven for 5 mins (50-60 degrees) > (5) remove from Coplin jar under a hood as formalin drops are now > carcinogenic vapour > (6) place slides in rack in oven so that the formalin moisture > dries... > (7) slide is ready for use > > Maria Katleba HT(ASCP), MS > Pathology Dept. Mgr. > Queen of the Valley Medical Center > 707-294-9229 cell > 707-252-4411 x3689 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net > Sent: Tuesday, April 27, 2010 12:17 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] toe nails falling off slides > > To all, > > > I was wondering if anyone out there had any tips on how to keep > toenail sections from falling off slides. We mount them on positive > slides, air dry for about an hour but they still fall off. Any > insight on the subject would be greatly appreciated. > > > Thanks, > > > Jenny > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Notice from St. Joseph Health System: > Please note that the information contained in this message may be > privileged and confidential and protected from disclosure. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity > to which it is addressed and may contain information that is > privileged and confidential. If the reader of this message is not > the intended recipient, please notify the sender immediately by > replying to this message and then delete it from your system. Any > review, dissemination, distribution, or reproduction of this message > by unintended recipients is strictly prohibited and may be subject > to legal restriction. > > > Thank you for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegg <@t> ihctech.net Wed Apr 28 10:13:35 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 28 10:14:22 2010 Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? In-Reply-To: <7EAF931905B1E440B21C11B545217E131E9495C1@SKMSG02.sickkids.ca> References: <466B666475DE6547BBB0641E540A4BB506876353BA@EXVS1.meriter.com> <4BD459FB.7400.0077.1@harthosp.org> <0D48EA46-525D-45A6-BFCD-24662D59FA07@mac.com>, <37212590E341574B96E6796D27DF42DAB04E95@RWDEX3.WHS.phci.org> <7EAF931905B1E440B21C11B545217E131B68C380@SKMSG02.sickkids.ca> <37212590E341574B96E6796D27DF42DAB04E9B@RWDEX3.WHS.phci.org> <7EAF931905B1E440B21C11B545217E131E9495C1@SKMSG02.sickkids.ca> Message-ID: <18710132BD4E451689E121B126D08823@Patsyoffice> Michael, Thanks for clarifying that. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Michael Ho Sent: Wednesday, April 28, 2010 9:04 AM To: Van Eyck, Deb; ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? In response to Debs post the other day I would like clarify that the PR antibody clone 312 cited in the new ASCO/CAP guidelines is in fact a product number for the Novocastra clone 16. Michael Ho Resource Technologist Immunopathology Division of Pathology DPLM The Hospital for Sick Children 555, University Avenue Toronto, Ontario, M5G1X8 Canada Tel# 416-813-5950 FAX#416-813-5974 From: Van Eyck, Deb [mailto:deb.vaneyck@phci.org] Sent: Monday, April 26, 2010 6:12 PM To: Michael Ho; ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? Thanks Michael ----I saw table 10 -but on page 16 they specifically only list clone 1294 and 312 (which is whose?) -they really don't clearly say all clones for PR listed in table 10. They also only refer to these two again on the shortened ASCO color plate handout Additional information in re: clinical question 2.1. Deb _____ From: Michael Ho [mailto:michael.ho@sickkids.ca] Sent: Monday, April 26, 2010 4:13 PM To: Van Eyck, Deb; ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? Hi Deb If you look at table 10 of the new guidelines you will see the ER/PR antibody clone list Michael _____ From: ihcrg@googlegroups.com [ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb [deb.vaneyck@phci.org] Sent: April 26, 2010 12:29 PM To: ancillarypath@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. _____ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. _____ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. From sjkitten <@t> live.com Wed Apr 28 10:31:04 2010 From: sjkitten <@t> live.com (S R) Date: Wed Apr 28 10:31:09 2010 Subject: [Histonet] Cold Plate Problems Message-ID: Hello everyone! So my cold plate does not cool anymore. Do any of you know anyone out there that services these. I called my normal biotech guy but since he thinks the compressor is not working then i would need someone who knew how to fix that. Thanks in Advance for your wonderful help Have a nice day Sammy _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4 From jcamper <@t> aerotek.com Wed Apr 28 10:57:03 2010 From: jcamper <@t> aerotek.com (Camper, Jack) Date: Wed Apr 28 10:57:11 2010 Subject: [Histonet] Histology Technician Job Opportunity Message-ID: We currently have a Histology Technician Position open in the Chicago-land area. The job is a first shift position. We are looking for any type of experience in histology as long as the candidate is good at cutting tissue. The interview will require hands on cutting. ASCP certification is must. Please reply to jcamper@aerotek.com if interested. Thanks, Jack Camper Recruiter Aerotek Medical 1933 N. Meacham Rd., Suite 400 Schaumburg, IL 60173 ( Work: 847-221-1665 ( Fax: 847-303-2370 * E-mail: jcamper@aerotek .com The greatest compliment to our business is a referral. If you know of someone who is looking for work, or who is hiring, please feel free to pass on my contact information. Thank You ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From jcamper <@t> aerotek.com Wed Apr 28 11:02:17 2010 From: jcamper <@t> aerotek.com (Camper, Jack) Date: Wed Apr 28 11:02:21 2010 Subject: [Histonet] Aerotek Healthcare Message-ID: We currently have a Histology Technician Position open in the Chicago-land area. The job is a first shift position. We are looking for any type of experience in histology as long as the candidate is good at cutting tissue. The interview will require hands on cutting. ASCP certification is must. Please reply to jcamper@aerotek.com if interested. Thanks, Jack Camper Recruiter Aerotek Medical 1933 N. Meacham Rd., Suite 400 Schaumburg, IL 60173 ( Work: 847-221-1665 ( Fax: 847-303-2370 * E-mail: jcamper@aerotek .com The greatest compliment to our business is a referral. If you know of someone who is looking for work, or who is hiring, please feel free to pass on my contact information. Thank You ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From Erin.Martin <@t> ucsf.edu Wed Apr 28 11:07:37 2010 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Apr 28 11:17:42 2010 Subject: [Histonet] Toenails Message-ID: <379A927A452F3D43A3C8705F4E67905F0FBB0F9460@EX05.net.ucsf.edu> Hi, We keep ours on by using egg albumin (from American Master Tech). Brush some on the slide, let it air dry then pick up your sections. It will hold through PASD as well as H&E. Good luck! Erin PS A 1:4 Nair solution softens the block up nicely after you face it in. Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From thomas.crowell <@t> novartis.com Wed Apr 28 10:57:30 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Wed Apr 28 11:19:13 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 04/28/2010 and will not return until 05/03/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From jcamper <@t> aerotek.com Wed Apr 28 11:27:42 2010 From: jcamper <@t> aerotek.com (Camper, Jack) Date: Wed Apr 28 11:27:46 2010 Subject: [Histonet] (no subject) Message-ID: We currently have a Histology Technician Position open in the Chicago-land area. The job is a first shift position. We are looking for any type of experience in histology as long as the candidate is good at cutting tissue. The interview will require hands on cutting. ASCP certification is must. Please reply to jcamper@aerotek.com if interested. Thanks, Jack Camper Recruiter Aerotek Medical 1933 N. Meacham Rd., Suite 400 Schaumburg, IL 60173 ( Work: 847-221-1665 ( Fax: 847-303-2370 * E-mail: jcamper@aerotek .com The greatest compliment to our business is a referral. If you know of someone who is looking for work, or who is hiring, please feel free to pass on my contact information. Thank You ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From thisisann <@t> aol.com Wed Apr 28 11:30:44 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Apr 28 11:31:15 2010 Subject: [Histonet] IHC Validation Procedure Message-ID: <8CCB50748D97178-1C98-92FF@webmail-d051.sysops.aol.com> Can anyone share their IHC Validation procedure. I am preparing for a CAP inspection and want to make sure that the procedure I (presently do/may change to) is acceptable. Thank you very much! From christina.thurby <@t> bms.com Wed Apr 28 11:43:55 2010 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Wed Apr 28 11:44:01 2010 Subject: [Histonet] Anyone doing Feulgen/Brdu Dual Labeling in Rodent Tissue? In-Reply-To: <90118214-24e8-4854-873f-fc244577a44d@ushpwbmsmhp001.one.ads.bms.com> References: <90118214-24e8-4854-873f-fc244577a44d@ushpwbmsmhp001.one.ads.bms.com> Message-ID: Hi, Is anyone out there doing Feulgen/BrdU Dual labeling? I have the procedure working (Blue Feulgen kit from Scytek) first and then commercially available BrdU antibody using standard ABC detection method. I am using a post antigen retrieval step after the Feulgen staining method is completed using Trilogy in the rice steamer. I do not let the antigen retrieval temp exceed 95 C in the rice steamer as I have been able to reproduce staining artifact when exceeding 95 C by just 2-3 C. I am having problems with reproducibility and labeling consistency within liver sections (1+ to 4+ zones). Please contact me if you have experience with this procedure and can help me troubleshoot! Thanks, Christina Thurby christina.thurby@bms.com Bristol Myers Squibb ph 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From zodiac29 <@t> comcast.net Wed Apr 28 11:46:53 2010 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Apr 28 11:46:56 2010 Subject: [Histonet] dimethyl sulfoxide Message-ID: <243877190.19570811272473213535.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> To All, Does anyone know where to purchase Dimethyl Sulfoxide for KOH preperation? Thanks in advance, Jenny From matt <@t> techoneweb.com Wed Apr 28 12:24:59 2010 From: matt <@t> techoneweb.com (matt@techoneweb.com) Date: Wed Apr 28 12:25:03 2010 Subject: [Histonet] re:Cold Plate Problems (S R) Message-ID: Hey Sammy, We can service them but they are generally considered throw away items. That's because if it is the compressor it will cost about $1500 to repair (+ shipping). If you are still interested, please contact me. Thanks Matt Mincer Tech One Biomedical Services 159 N Marion Street PMB163 Oak Park, IL 60301 708-383-6040 X 10 From sweething63 <@t> msn.com Wed Apr 28 12:41:59 2010 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Wed Apr 28 12:42:03 2010 Subject: [Histonet] New email address In-Reply-To: <4BD702CA020000820004FDB3@GWIA1.umsmed.edu> References: <4BD702CA020000820004FDB3@GWIA1.umsmed.edu> Message-ID: You can do it yourself by clicking on the same link that you can unsubscribe on. That's how I did it when I switched it to my home email. > Date: Tue, 27 Apr 2010 15:29:14 -0500 > From: dholmes@anatomy.umsmed.edu > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] New email address > > Please change my email address to www.DHolmes@umc.edu > Thank you > > > > Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcamper <@t> aerotek.com Wed Apr 28 12:48:08 2010 From: jcamper <@t> aerotek.com (Camper, Jack) Date: Wed Apr 28 12:48:14 2010 Subject: [Histonet] Aerotek Healthcare Message-ID: We currently have a Histology Technician Position open in the Chicago-land area. The job is a first shift position. We are looking for any type of experience in histology as long as the candidate is good at cutting tissue. The interview will require hands on cutting. ASCP certification is must. Please reply to jcamper@aerotek.com if interested. Thanks, Jack Camper Aerotek Healthcare ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From kimtournear <@t> yahoo.com Wed Apr 28 13:06:16 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Apr 28 13:06:21 2010 Subject: [Histonet] Charged Slides for IHC on Ventana In-Reply-To: Message-ID: <660202.334.qm@web54205.mail.re2.yahoo.com> we use fisher slides, but I hear that Cardinal distibutes them at a reasonable price... ? ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Wed, 4/28/10, Jack Dodo wrote: From: Jack Dodo Subject: RE: [Histonet] Charged Slides for IHC on Ventana To: wbenton@umm.edu, histonet@lists.utsouthwestern.edu Date: Wednesday, April 28, 2010, 7:21 AM We have been using a remarkably priced plus slide from Epic with GREAT success. Call Epic Scientific at 888-795-8300, the price and performance will shock you. Good Luck. > Date: Wed, 21 Apr 2010 13:22:54 -0400 > From: WBENTON@umm.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Charged Slides for IHC on Ventana > > Message: 2 > Date: Tue, 20 Apr 2010 10:27:57 -0700 > From: srishan@mail.holyname.org > Subject: [Histonet] (no subject) > To: histonet-bounces@lists.utsouthwestern.edu, > > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Hi All, > > We are using Ventana XT for our immunostaining. We tried using "Plus" > slides made by many vendors. The current vendor's pricing is way too > high! With other vendors slides we have been having problems. Please > let me know where to get some plus slides which are reasonable. I know > the recommended slides come from erie but I am not quite sure about > vendors who carry it and how much they charge. > > Thanks > > Nirmala Srishan > Histology > Holy Name Medical Center. > > Nirmala, > > We use Fisher's charged slides 12-550-15, which I'm sure are made by Erie. Pricing will depend upon your contract with a particular vendor. SurgiPath aka Leica has charged slides that work well also. If you want the etched Control Box Slides we purchase from Cardinal. > Hope this helps! > > > Walter Benton, HT(ASCP)QIHC > Histology Supervisor > University of Maryland Medical Center > Anatomic Pathology > 22 S. Greene St > Room NBW65 > Baltimore MD 21201 > (Direct) 410-328-0930 > (Lab) 410-328-5524 > (Fax) 410-328-5508 > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Wed Apr 28 13:47:05 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Apr 28 13:47:24 2010 Subject: [Histonet] Responses to IHC CAP Validation question Message-ID: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com> The following is one respone I rec'd: 1. I asked CAP who told me that they do not currently have a guideline on validating but that they recommend what is in the following book: Quality Management In Anatomic Pathology, Promoting Patient Safety Through Systems Improvement and Error by Raouf E. Nakhleh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8- Quality Management in IHC That is what we follow. I. Get a new antibody and optimize it with your positive control. II. Once optimized you need to run it on cases expected to be positive (how many?) "a suffient size ..." III. Must also be run on cases expected to be negative. (how many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just that. (ex. some of the hormones we just use a pituitary) From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Apr 28 14:01:57 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Apr 28 14:06:00 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com> References: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com> Message-ID: Any inspection that I have undergone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2. We use closer to 50 cases. We also use a TMA to make our lives easier. The TMA contains known positives and known negatives. In cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases is easy. But when you are validated for more hard to find markers (SV-40) then fewer cases is acceptable. We always throw in a slide that we know will not stain for sv-40 like a tonsil - then you can say it has specificity. Any inspector that I have come across is usually understanding of this. But I am sure that there are exceptions to this.........especially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 2010 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Responses to IHC CAP Validation question The following is one respone I rec'd: 1. I asked CAP who told me that they do not currently have a guideline on validating but that they recommend what is in the following book: Quality Management In Anatomic Pathology, Promoting Patient Safety Through Systems Improvement and Error by Raouf E. Nakhleh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8- Quality Management in IHC That is what we follow. I. Get a new antibody and optimize it with your positive control. II. Once optimized you need to run it on cases expected to be positive (how many?) "a suffient size ..." III. Must also be run on cases expected to be negative. (how many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just that. (ex. some of the hormones we just use a pituitary) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Wed Apr 28 14:10:25 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 28 14:18:05 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DEA@is-e2k3.grhs.net> I know you keep the paper work showing what you have done, but do you keep slides also? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Wednesday, April 28, 2010 2:02 PM To: thisisann@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Responses to IHC CAP Validation question Any inspection that I have undergone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2. We use closer to 50 cases. We also use a TMA to make our lives easier. The TMA contains known positives and known negatives. In cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases is easy. But when you are validated for more hard to find markers (SV-40) then fewer cases is acceptable. We always throw in a slide that we know will not stain for sv-40 like a tonsil - then you can say it has specificity. Any inspector that I have come across is usually understanding of this. But I am sure that there are exceptions to this.........especially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 2010 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Responses to IHC CAP Validation question The following is one respone I rec'd: 1. I asked CAP who told me that they do not currently have a guideline on validating but that they recommend what is in the following book: Quality Management In Anatomic Pathology, Promoting Patient Safety Through Systems Improvement and Error by Raouf E. Nakhleh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8- Quality Management in IHC That is what we follow. I. Get a new antibody and optimize it with your positive control. II. Once optimized you need to run it on cases expected to be positive (how many?) "a suffient size ..." III. Must also be run on cases expected to be negative. (how many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just that. (ex. some of the hormones we just use a pituitary) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.a.troutman <@t> Vanderbilt.Edu Wed Apr 28 14:19:59 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Wed Apr 28 14:20:03 2010 Subject: [Histonet] RE: Histonet Digest, Vol 77, Issue 36 In-Reply-To: <201004281701.o3SH11cU009603@mailgate03> References: <201004281701.o3SH11cU009603@mailgate03> Message-ID: <7B310892042DA74CB3590053F424CFE60B83F96FBD@ITS-HCWNEM06.ds.Vanderbilt.edu> Hello, We do a two stage validation procedure here. First, I will work up an antibody to get a working protocol. I give the best slides along with a form recording the processes used for each slide (dilution, antigen retrieval solution, A-R time, chromogen, antibody incubation time, etc.) and I make the pathologist sign off on the one they like the best. Next, we pull a number of cases (we do 10-15) that include known positives and negatives. (If you are switching platforms, just pull the old slides and compare them to the new method.) Record this data (block numbers, etc.) along with the pathologist's signature. If it is a new antibody, I have to tell the pathologist to find a minimum of 10 cases that the stain will be used on and I can validate it that way. If you are validating ER, PR and/or Her-2, you are in for a lot more work. ASCO/CAP recommends 20 to 100 cases that must be compared to a known method. ie someone else's lab or your old method. We did 50 and it took awhile. Sorry this is a bit choppy. Feel free to email me with any other specific questions that you might have or if you want the forms I use. We are in the same boat! CAP is due any day, now! Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 Message: 7 Date: Wed, 28 Apr 2010 12:30:44 -0400 From: thisisann@aol.com Subject: [Histonet] IHC Validation Procedure To: histonet@lists.utsouthwestern.edu Message-ID: <8CCB50748D97178-1C98-92FF@webmail-d051.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can anyone share their IHC Validation procedure. I am preparing for a CAP inspection and want to make sure that the procedure I (presently do/may change to) is acceptable. Thank you very much! From liz <@t> premierlab.com Wed Apr 28 14:44:39 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Apr 28 14:44:44 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: <8CCB51A55105D80-1A38-1C45@webmail-m088.sysops.aol.com> Message-ID: CAP does have guidelines, it's a paper that was published a few years back, its right on the CAP website. I have it if anyone needs it. I'm not sure they use it for their inspections but it covers how to validate an antibody. How many samples to run, what paperwork needs to be kept, etc. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Wednesday, April 28, 2010 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Responses to IHC CAP Validation question The following is one respone I rec'd: 1. I asked CAP who told me that they do not currently have a guideline on validating but that they recommend what is in the following book: Quality Management In Anatomic Pathology, Promoting Patient Safety Through Systems Improvement and Error by Raouf E. Nakhleh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8- Quality Management in IHC That is what we follow. I. Get a new antibody and optimize it with your positive control. II. Once optimized you need to run it on cases expected to be positive (how many?) "a suffient size ..." III. Must also be run on cases expected to be negative. (how many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just that. (ex. some of the hormones we just use a pituitary) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Apr 28 15:20:52 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Apr 28 15:26:51 2010 Subject: [Histonet] Responses to IHC CAP Validation question In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3DEA@is-e2k3.grhs.net> References: , <661949901A768E4F9CC16D8AF8F2838C017A3DEA@is-e2k3.grhs.net> Message-ID: I keep the slides to. They are good references to look at for future stainng issues. Otherwise they just sit on the shelf in a box. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: Mike Pence [mpence@grhs.net] Sent: Wednesday, April 28, 2010 3:10 PM To: McMahon, Loralee A; thisisann@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Responses to IHC CAP Validation question I know you keep the paper work showing what you have done, but do you keep slides also? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Wednesday, April 28, 2010 2:02 PM To: thisisann@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Responses to IHC CAP Validation question Any inspection that I have undergone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2. We use closer to 50 cases. We also use a TMA to make our lives easier. The TMA contains known positives and known negatives. In cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases is easy. But when you are validated for more hard to find markers (SV-40) then fewer cases is acceptable. We always throw in a slide that we know will not stain for sv-40 like a tonsil - then you can say it has specificity. Any inspector that I have come across is usually understanding of this. But I am sure that there are exceptions to this.........especially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com [thisisann@aol.com] Sent: Wednesday, April 28, 2010 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Responses to IHC CAP Validation question The following is one respone I rec'd: 1. I asked CAP who told me that they do not currently have a guideline on validating but that they recommend what is in the following book: Quality Management In Anatomic Pathology, Promoting Patient Safety Through Systems Improvement and Error by Raouf E. Nakhleh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8- Quality Management in IHC That is what we follow. I. Get a new antibody and optimize it with your positive control. II. Once optimized you need to run it on cases expected to be positive (how many?) "a suffient size ..." III. Must also be run on cases expected to be negative. (how many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just that. (ex. some of the hormones we just use a pituitary) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aallison <@t> wpalabs.com Wed Apr 28 16:22:18 2010 From: aallison <@t> wpalabs.com (aallison@wpalabs.com) Date: Wed Apr 28 16:22:27 2010 Subject: [Histonet] New Histology Position Opportunity in Phoenix,AZ Message-ID: <20100428142218.85fdaa4bc40941c5dd00a74b80284d9b.a1cca23ace.wbe@email04.secureserver.net> Western Pathology Associates, Ltd, a pathology reference laborato has an full time opening for a Histotechnologist for the day shift , Monday through Friday with No weekends. We are a new state of the art facility located next to John C. Lincoln metropolitan h The successful candidate must have at least 4 plus years of experience being a tea Intellepath I based on the overa We offer an excelle full health car W job. To apply Please Contact: Akemi Allison BS, HT (ASCP) HTL Manager, Anatomical Pathology Western Pathology Associates Tele: 602.633.3800 X 306 Fax: 602.861.3500 Cell: 408.335.9994 E-Mail: [1]aallison@wpalabs.com References 1. 3D"mailto:aallison@wpalabs.com" From Vickroy.Jim <@t> mhsil.com Wed Apr 28 16:24:51 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Apr 28 16:25:00 2010 Subject: [Histonet] microwave tissue Message-ID: <24A4826E8EF0964D86BC5317306F58A54258B78BCB@mmc-mail.ad.mhsil.com> We have just noticed an issue with our small biopsies lately. We are processing most of the small biopsies by a microwave processor. We finished our validation process about a couple of weeks ago and found little difference between the microwave processed tissue verses conventional processing. Now the pathologists are telling us that the nuclei do not seem as sharp and crisp as before. They of course think that the lack of sharpness is due to microwaving but obviously there are other issues to consider. We stain all of our biopsies on an automated stainer. Tomorrow we will again run some biopsies on the microwave and some on the conventional processor and see if there are any noticeable difference. Obviously if we see that the microwave tissue is not as crisp then we may need to adjust our microwave processing program. Another variable however was added about two weeks ago. We switched from Citrisolve to Clearite III on our stainer. Has anybody experienced similar issues with Clearite III on their automated stainer? Any other ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From pathmaster <@t> yahoo.com Wed Apr 28 16:59:11 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Wed Apr 28 16:59:17 2010 Subject: [Histonet] Patient identifiers and odds and ends Message-ID: <187517.59758.qm@web111108.mail.gq1.yahoo.com> Bill is quite right and I was wrong to lump State and CAP together in requiring two identifiers at all steps. But finding room for two tiny initials on the cassette and in process slide is not contortionist and has served us quite well in avoiding errors. By the way, just give them a little more time and they'll require two identifiers on cassettes soon enough, I'm sure. NY State is quite big on risk assessment for misidentification since some well publicized breast core biopsy mix-ups resulting in unneeded mastectomies. The main and perhaps the only reasons to examine toenails are to find fungal infections or maybe a subungual melanoma. Jeff Silverman From napoli <@t> siscom.net Wed Apr 28 18:03:54 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Wed Apr 28 18:03:59 2010 Subject: [Histonet] 379A927A452F3D43A3C8705F4E67905F0FBB0F9460@EX05.net.ucsf.edu Message-ID: <4bd8beda.1f1.7e7c.1500727391@siscom.net> Nails Finger or toenail sections can best be processed either through the procedures noted by Joe Nocito on this forum (HI JOE!) or by using a method I use (which is similar. Soften nail fragments by placing (after fixation) them into a solution of 20% or 10% sodium hydroxide or 20%/10% solution of potassium hydroxide. Process as normal after frags become pliable. Embed, face blocks and surface soften with 10% KOH again just before rinsing off and sectioning. I suggest using plus slide or adhesive slides. Keep in mind that during this process it is possible to have a melanoma underneath a nail that must be paid attention to if the pre-operative/clinical dx states r/o MM etc. In case there is more than fungal disorder (onychomycosis) being diagnosed an iron stain and a melanin stain may be useful as well. Additionally, keep in mind that if there is soft tissue attached to the nail, it is possible to destroy the architecture of the cells if they are treated with too caustic of a substance. Sometimes soft tissue frags will also be detachable or will be detached in bottle. Hope this helps. Andrew B Dermatotechniques From napoli <@t> siscom.net Wed Apr 28 18:12:31 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Wed Apr 28 18:12:35 2010 Subject: [Histonet] 379A927A452F3D43A3C8705F4E67905F0FBB0F9460@EX05.net.ucsf.edu Message-ID: <4bd8c0df.1d9.368.553534850@siscom.net> Something I forgot to state in the procedure for nails: heat slides in 88 degree oven for 20 mins and cool before staining. Andrew B. From mpence <@t> grhs.net Thu Apr 29 08:09:00 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Apr 29 08:09:20 2010 Subject: [Histonet] microwave tissue In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54258B78BCB@mmc-mail.ad.mhsil.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3DEB@is-e2k3.grhs.net> Give your pathologist a blind study and have them tell you which are microwave and which are conventional processed. This will tell you "where" the problem lies! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, April 28, 2010 4:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave tissue We have just noticed an issue with our small biopsies lately. We are processing most of the small biopsies by a microwave processor. We finished our validation process about a couple of weeks ago and found little difference between the microwave processed tissue verses conventional processing. Now the pathologists are telling us that the nuclei do not seem as sharp and crisp as before. They of course think that the lack of sharpness is due to microwaving but obviously there are other issues to consider. We stain all of our biopsies on an automated stainer. Tomorrow we will again run some biopsies on the microwave and some on the conventional processor and see if there are any noticeable difference. Obviously if we see that the microwave tissue is not as crisp then we may need to adjust our microwave processing program. Another variable however was added about two weeks ago. We switched from Citrisolve to Clearite III on our stainer. Has anybody experienced similar issues with Clearite III on their automated stainer? Any other ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Thu Apr 29 08:19:12 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Apr 29 08:19:19 2010 Subject: [Histonet] MAILING BIOPSIES Message-ID: HISTONETTERS I was wondering if there are any REGS out formalin filled biopsies thru UPS or FED EX other than package and the spill pad. Anyone have any knowledge about the subject? Thanks Pathology Supervisor Kathy Ba Memorial Hospital and Health& [1]sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, &nbs Pager 812-481-0897 < References 1. 3D"mailto:sbaldwin@mhhcc.org" From b-frederick <@t> northwestern.edu Thu Apr 29 09:26:15 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 29 09:26:47 2010 Subject: [Histonet] MAILING BIOPSIES In-Reply-To: Message-ID: <54AC4CDC4D024FE2BA6CCA9425B47FFF@lurie.northwestern.edu> Sarah, The IATA (International air transport association) has all the rules for shipping and receiving. of biological substances (UN3373). We have to be certified every year as we are a biorepository and ship and receive from all over the world. We get blocks, slides serum, plasma, whole blood and extracted DNA ,so we have to know what to do with it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, April 29, 2010 8:19 AM To: histonet Subject: [Histonet] MAILING BIOPSIES HISTONETTERS I was wondering if there are any REGS out =here about mailing formalin filled biopsies thru UPS or FED EX other than =he special package and the spill pad. Anyone have any knowledge about the subject? Thanks Pathology Supervisor Kathy Ba=dwin, SCT (ASCP) Memorial Hospital and Health&=bsp;Care Center [1]sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, &nbs=;Fax 812-482-0232, Pager 812-481-0897 <=R> Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhcc.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Apr 29 10:16:05 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Apr 29 10:16:12 2010 Subject: [Histonet] "field" histology - how to do it? Message-ID: <0861A72E-CF0D-4ABD-96D8-7FEDAB8F5C92@email.arizona.edu> Fellow Histonetters, I'm preparing to accompany a group of dentists and doctors (including a dermatologist) to Belize in a few months. They have decided to add histology to what they are offering the people down there and I have been asked to participate. Has anybody ever done anything like this and/or can anybody offer any suggestions as to what we might need to set up our histology lab. Right now I know that there are a few clinics around the country and what they are doing is sending tissues to a lab in Miami. The poor people don't have access or very much access to the clinics and that is why we are going to do this. We may have to work in several locations or collect our tissues and take them back to a central location - we aren't sure yet. My questions center around the equipment we will need to take into Belize and the chemicals and supplies. Since we are doing this for the first time it will surely be a learning experience. Would you suggest paraffin sections, microwave processing, frozens? Any ideas will be welcome. Thank you! Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. From canyon <@t> path-tec.com Thu Apr 29 10:18:14 2010 From: canyon <@t> path-tec.com (Canyon Bowie) Date: Thu Apr 29 10:18:36 2010 Subject: [Histonet] MAILING BIOPSIES References: Message-ID: <01f001cae7af$3003d110$900b7330$@com> There are different regulations for the classification of your specimens. DOT and IATA are the guidelines to follow, and then FedEx & UPS have their specific additional regulations. If your specimens are suspected or known to be infectious substances, then you will need to adhere to Class B UN3373 regulations, however if they are not, then "Exempt Human Specimen" would be the regulations to follow. If anyone would like I have PDF's of the DOT/IATA regulations (about 2 pages) for each of the classifications that I can email you, I also have the FedEx guidelines (which basically repeat the DOT/IATA guidelines, but add lab packs and such.) This is what my company specializes in - creating custom specimen collection kits for labs, helping them to get them to their clients and back into the lab while meeting shipping regulations. Feel free to contact me for more info, you can also send me your current kits and we will do a free kit evaluation for you to ensure that you are meeting regulations. For example a lot of labs don't realize that using Chip Boards (very thin cardboard sleeve mailers do not meet regulations.) Canyon Bowie Path-Tec "The Lab Kit Specialists" w. 706.507.1575 / f. 706.569.6369 canyon@path-tec.com www.path-tec.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, April 29, 2010 9:19 AM To: histonet Subject: [Histonet] MAILING BIOPSIES HISTONETTERS I was wondering if there are any REGS out =here about mailing formalin filled biopsies thru UPS or FED EX other than =he special package and the spill pad. Anyone have any knowledge about the subject? Thanks Pathology Supervisor Kathy Ba=dwin, SCT (ASCP) Memorial Hospital and Health&=bsp;Care Center [1]sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, &nbs=;Fax 812-482-0232, Pager 812-481-0897 <=R> Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhcc.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Thu Apr 29 10:29:16 2010 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Apr 29 10:29:24 2010 Subject: [Histonet] Processing problem Message-ID: <379A927A452F3D43A3C8705F4E67905F0FBB0F946B@EX05.net.ucsf.edu> Hi everyone, We are having a problem with a processing artifact that looks like very pale staining in splotches just above the junction between the epidermis and dermis but not extending all the way to the skin surface, and not everywhere at the position. One of the processors had a clog in a line a few weeks ago that was causing reagents to heavily carry over into each other so I am suspicious that this may be the same problem recurring but our pathologist thinks that the tissue is dessicated. I have photomicrographs to share if anyone has any ideas... Thank you in advance for your help! Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From NSEARCY <@t> swmail.sw.org Thu Apr 29 10:59:47 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Apr 29 10:59:57 2010 Subject: [Histonet] Histology Technician Opening Message-ID: <4BD966A3.5D38.00EF.0@swmail.sw.org> Scott & White Hospital, a Multispecialty hospital and clinic serving as the primary clinical teaching facilities for Texas A&M University College of Medicine in Temple, Texas has an opening in the histology laboratory for a histology technician. Today, Scott & White Healthcare is a fully integrated health system * the largest multi-specialty practice in Texas and the sixth largest group practice in the nation. Scott & White employs more than 800 physicians and research scientists who care for patients covering 25,000 square miles across. To learn more about S&W, ( www.sw.org) and the position, contact Patricia Webster, Lead Technician @ 254-724-6938. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From cbrya <@t> lexclin.com Thu Apr 29 11:02:22 2010 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Apr 29 11:02:29 2010 Subject: [Histonet] inconsistent staining after quarterly decon Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF0595E96117@EXCHANGESB> Has anyone else ever experienced inconsistent staining on immunos after the quarterly decontamination of their Benchmark? I think the Lysol IC is still in the lines after numerous water flushes. Thank you for any input or suggestions. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From rmweber113 <@t> comcast.net Thu Apr 29 11:37:13 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Apr 29 11:37:16 2010 Subject: [Histonet] Cost per slide Message-ID: <2079250832.23385621272559033349.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Hello,? Does anyone know how much it costs to run a slide for urine cytology (cytospin). Thanks, From kimtournear <@t> yahoo.com Thu Apr 29 11:46:21 2010 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Apr 29 11:46:25 2010 Subject: [Histonet] inconsistent staining after quarterly decon In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF0595E96117@EXCHANGESB> Message-ID: <644648.15010.qm@web54203.mail.re2.yahoo.com> Hi Carol, We use?(2) benchmarks in our lab but use Amphyl to decon. After?decon, we?flush with water?by "priming" the systems and then?we?"purge"?reaction buffer and ez prep?fluid back into the lines. ? Purge alcohol thru the liquid coverslip lines to make sure all the water evaporates out of the lines. Before purging the LCS into?the lines make sure the?LCS container dry completely before refilling.?I?suggesting rinsing?container with 100% alcohol then wait 15-20?minutes before introducing LCS back into the system. ? I would suggest you cut 20 full coverage tonsil slides (sections from top to bottom) and run all 20 of them with?the same?antibody to test your staining (CD20 will work nicely). This will tell you if you are getting even coverage from the top of the slide to the bottom and side to side. It might not be the decon procedure, it might be something else. ? Hope this helps!! ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Thu, 4/29/10, Carol Bryant wrote: From: Carol Bryant Subject: [Histonet] inconsistent staining after quarterly decon To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, April 29, 2010, 9:02 AM Has anyone else ever experienced inconsistent staining on immunos after the quarterly decontamination of their Benchmark?? I think the Lysol IC is still in the lines after numerous water flushes. Thank you for any input or suggestions. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.? If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.? Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Apr 29 12:47:03 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Apr 29 12:47:08 2010 Subject: [Histonet] FISH Validation Message-ID: <812231.80910.qm@web113812.mail.gq1.yahoo.com> Hello All in Histoland, ? I am once again requesting your expertise in the Molecular FISH world of validation.? I am treading on unfamiliar territory here.? ? I am very familiar with validation for histology and IHC, but not FISH.? I was requested to gather information regarding how many cases are required to meet CAP requirements?for FISH tests.? Our FISH tests are contracted out to someone who is using our facility to perform the tests.? They are going to bring on board several new FISH tests and we want to make sure everything is within the guidelines that CAP has set-up. ? We will be inspected by CAP in the next few months, and we want to make sure we comply to their requirements.? ? Thank you in advance for your assistance. ? Akemi Allison BS, HT (ASCP) HTL Manager, Anatomical Pathology Western Pathology Associates Tele: 602.633.3800 X 306 Fax: 602.861.3500 Cell: 408.335.9994 E-Mail: aallison@wpalabs.com From HParker <@t> Skaggs.Net Thu Apr 29 15:05:33 2010 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu Apr 29 15:05:01 2010 Subject: [Histonet] Billing for Prostate Biopsies In-Reply-To: <20100427170555.CB29431A0EB@barracuda.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F5060042B35EDF0@email1.skaggs.net> Can anyone tell me what/how prst bx are billed ?? Helayne Parker, HT (A.S.C.P.) From LRaff <@t> uropartners.com Thu Apr 29 15:09:02 2010 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Apr 29 15:09:06 2010 Subject: [Histonet] Billing for Prostate Biopsies In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F5060042B35EDF0@email1.skaggs.net> References: <20100427170555.CB29431A0EB@barracuda.skaggs.net> <930EB2E8DF68C544873EDD2A3D5F5060042B35EDF0@email1.skaggs.net> Message-ID: Each separately submitted jar with a specified location is coded as 88305, regardless of the number of cores in the jar. An exception is for "saturation" biopsies (ultrasound guided template needle biopsies) that are coded using 4 specific G codes, depending on total number of cores. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Thursday, April 29, 2010 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Billing for Prostate Biopsies Can anyone tell me what/how prst bx are billed ?? Helayne Parker, HT (A.S.C.P.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET NOD32 Antivirus, version of virus signature database 5073 (20100429) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 5073 (20100429) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From Sara.Phinney <@t> leica-microsystems.com Thu Apr 29 17:21:47 2010 From: Sara.Phinney <@t> leica-microsystems.com (Sara.Phinney@leica-microsystems.com) Date: Thu Apr 29 17:21:58 2010 Subject: [Histonet] Phinney, Sara is out of the office. Message-ID: I will be out of the office starting 04/29/2010 and will not return until 05/03/2010. I will be out of the office on Friday April 30 and returning Monday, April 3. If you require technical assistance please dial 1-800-248-0123. For all other matters please call Timm Piper at 617-653-2605. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cdbeads <@t> earthlink.net Thu Apr 29 23:40:40 2010 From: cdbeads <@t> earthlink.net (Daniel Adams) Date: Thu Apr 29 23:37:33 2010 Subject: [Histonet] Slide and Block Storage Message-ID: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan From flnails <@t> texaschildrens.org Fri Apr 30 07:11:44 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Apr 30 07:12:32 2010 Subject: [Histonet] Slide and Block Storage In-Reply-To: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> References: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> Message-ID: If you are supplying the professional component, then it is a good idea to maintain the blocks and slides. If you are inspected and they do an audit trail you must be able to produce the slides and/or blocks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Thursday, April 29, 2010 11:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From canyon <@t> path-tec.com Fri Apr 30 07:51:52 2010 From: canyon <@t> path-tec.com (Canyon Bowie) Date: Fri Apr 30 07:52:15 2010 Subject: [Histonet] MAILING BIOPSIES In-Reply-To: <54AC4CDC4D024FE2BA6CCA9425B47FFF@lurie.northwestern.edu> References: <54AC4CDC4D024FE2BA6CCA9425B47FFF@lurie.northwestern.edu> Message-ID: <003201cae863$e7f52710$b7df7530$@com> Hi All, There has been quite a large response to the shipping regulations of mailing specimens, as I would expect to the guidelines changing consistently. I will be at the CLMA show in Vegas on May 4th and 5th. Please feel free to stop by at booth #626 and I will be available to answer more questions regarding the regulations and make suggestions for you. I will also have samples of different specimen mailing kits. You can bring me (or mail me) your current kits and we will evaluate them for free, letting you know if they meet regulations and if other improvements can be made. Thanks, Canyon Bowie Path-Tec 3151 Williams Rd Bldg C Columbus, GA w. 706.507.1575 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, April 29, 2010 8:19 AM To: histonet Subject: [Histonet] MAILING BIOPSIES HISTONETTERS I was wondering if there are any REGS out =here about mailing formalin filled biopsies thru UPS or FED EX other than =he special package and the spill pad. Anyone have any knowledge about the subject? Thanks Pathology Supervisor Kathy Ba=dwin, SCT (ASCP) Memorial Hospital and Health&=bsp;Care Center [1]sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, &nbs=;Fax 812-482-0232, Pager 812-481-0897 <=R> Confidential information, Authorized use only. References 1. 3D"mailto:sbaldwin@mhhcc.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Fri Apr 30 07:51:39 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Apr 30 07:52:19 2010 Subject: [Histonet] Slide and Block Storage In-Reply-To: References: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> Message-ID: I am able to reply to questions n the histonet but I am unable to post anything on the histonet. Is there a different address I should be using instead of histonet@list.utsouthwestern.edu? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, April 30, 2010 7:12 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If you are supplying the professional component, then it is a good idea to maintain the blocks and slides. If you are inspected and they do an audit trail you must be able to produce the slides and/or blocks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Thursday, April 29, 2010 11:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From ekronenberger <@t> sbcglobal.net Fri Apr 30 09:40:25 2010 From: ekronenberger <@t> sbcglobal.net (Elizabeth Kronenberger) Date: Fri Apr 30 09:40:15 2010 Subject: [Histonet] Slide and Block Storage In-Reply-To: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> References: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> Message-ID: If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Fri Apr 30 09:47:47 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Apr 30 09:48:23 2010 Subject: [Histonet] Slide and Block Storage In-Reply-To: References: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> Message-ID: So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From ekronenberger <@t> sbcglobal.net Fri Apr 30 10:00:04 2010 From: ekronenberger <@t> sbcglobal.net (Elizabeth Kronenberger) Date: Fri Apr 30 10:00:18 2010 Subject: [Histonet] Slide and Block Storage In-Reply-To: References: <742A5C6D-63D0-49CE-8757-E878CDE181DD@earthlink.net> Message-ID: <2206BD35B2444BBA8DBF793EF14858F9@dlne.local> Clients fax orders for deepers and special stains which we perform and send them out with their next package for them to read and report. -----Original Message----- From: Nails, Felton [mailto:flnails@texaschildrens.org] Sent: Friday, April 30, 2010 10:48 AM To: 'Elizabeth Kronenberger'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================ == From cpyse <@t> x-celllab.com Fri Apr 30 10:48:54 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Apr 30 10:50:05 2010 Subject: [Histonet] CD68 Message-ID: <000301cae87c$a33b6d50$e9b247f0$@com> Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From marktarango <@t> gmail.com Fri Apr 30 10:54:20 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 30 10:54:26 2010 Subject: [Histonet] CD68 In-Reply-To: <000301cae87c$a33b6d50$e9b247f0$@com> References: <000301cae87c$a33b6d50$e9b247f0$@com> Message-ID: Hi Cindy, My lab uses KP-1. Mark On Fri, Apr 30, 2010 at 8:48 AM, Cynthia Pyse wrote: > Happy Friday Everyone > > What clone is everyone using for the CD68 antibody for FFPE human tissue? > Thanks for the info in advance. Everyone have a great weekend. > > > > Cindy Pyse, CLT, HT (ASCP) > > Histology Supervisor > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SDrew <@t> uwhealth.org Fri Apr 30 11:11:32 2010 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Fri Apr 30 11:11:39 2010 Subject: [Histonet] CD68 In-Reply-To: <000301cae87c$a33b6d50$e9b247f0$@com> References: <000301cae87c$a33b6d50$e9b247f0$@com> Message-ID: <738A7878143FF74BB77436E255743C1A01000500@UWHC-MAIL03.uwhis.hosp.wisc.edu> We also use KP1 Sally -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Fri Apr 30 13:15:42 2010 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Apr 30 13:16:14 2010 Subject: [Histonet] CD68 Message-ID: We are currently using KP-1 and HAM 56 Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Drew Sally A 04/30/10 12:11 PM >>> We also use KP1 Sally -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gankam <@t> googlemail.com Fri Apr 30 13:32:58 2010 From: gankam <@t> googlemail.com (Fabrice gankam) Date: Fri Apr 30 13:33:08 2010 Subject: [Histonet] CD68 In-Reply-To: <000301cae87c$a33b6d50$e9b247f0$@com> References: <000301cae87c$a33b6d50$e9b247f0$@com> Message-ID: I use the ED1 from abdserotec and it works perfectly can dilute up to 1/500 to 1/1000 2010/4/30 Cynthia Pyse > Happy Friday Everyone > > What clone is everyone using for the CD68 antibody for FFPE human tissue? > Thanks for the info in advance. Everyone have a great weekend. > > > > Cindy Pyse, CLT, HT (ASCP) > > Histology Supervisor > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kevin_Kurtz <@t> ssmhc.com Fri Apr 30 13:46:16 2010 From: Kevin_Kurtz <@t> ssmhc.com (Kevin_Kurtz@ssmhc.com) Date: Fri Apr 30 13:46:27 2010 Subject: [Histonet] Repeat validation when IHC protocol changed? Message-ID: Recently our IHC vendor sent a tech to help us out with some weak staining affecting multiple antibodies. Several staining protocols were changed, including adjusting the incubation time and adding amplification. The results were great in the slides that I was shown. The tech said that revalidation was unnecessary, since the changes to the protocol were considered "minor". However, I personally disagree, since we don't know how the change in the staining protocol could potentially affect staining of other tissues (especially tissues that would be expected to be negative). Before subjecting our lab to the cost and effort of revalidation, I'd like to get your opinion. For new antibodies, I usually validate using 10 cases expected to be positive and 10 cases expected to be negative for the antibody in question. For revalidation, I was thinking of running 5 positive cases and 5 negative cases. Thanks for your input - Kevin Kurtz, M.D. St. Mary's Hospital Madison, Wisconsin Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From pathmaster <@t> yahoo.com Fri Apr 30 13:47:17 2010 From: pathmaster <@t> yahoo.com (Jeffrey Silverman) Date: Fri Apr 30 13:47:21 2010 Subject: [Histonet] CD68??` Message-ID: <821799.98032.qm@web111111.mail.gq1.yahoo.com> Another vote for KP-1. It also stains mast cells. Jeff Silverman From tyler.liebig <@t> thermofisher.com Fri Apr 30 14:40:45 2010 From: tyler.liebig <@t> thermofisher.com (Liebig, Tyler K.) Date: Fri Apr 30 14:40:46 2010 Subject: [Histonet] disposable blade holder Message-ID: <6746115DFA761840A605BB27624424AAEF0F10D5@USPHO-MXVS01.amer.thermo.com> Hello Lucy, In regards to your trouble finding an HM310 blade holder we do have one in our refurbished pool that I could have sent to you. It is used but in working order so we can let you have it at no charge. If you let me know where to ship it I will have it sent. Hope that helps, It's hard to cut without a blade holder. Regards, Tyler Liebig Product Manager Histology Instrumentation and IHC Anatomical Pathology Thermo Fisher Scientific 46360 Fremont Blvd Fremont, CA 94538 USA Office: (510) 979-5000 Mobile: (510) 299-1751 Fax: (510) 979-5239 tyler.liebig@thermofisher.com www.thermo.com From vrodriguez10 <@t> gmail.com Fri Apr 30 19:34:39 2010 From: vrodriguez10 <@t> gmail.com (Valerie R.) Date: Fri Apr 30 19:35:05 2010 Subject: [Histonet] How to troubleshoot a cytology slide with dehydration problems Message-ID: I am an HTL but I have to stain pap smears. In the Papanicalou procedure for fine needle aspirations I have to stain the slides in sodium chloride first for 25 seconds in total (15 seconds in one container and 10 in another container). This for me is the trickiest part when staining cytology because if slides are not well dehydrated in saline then the cells are not going to absorb the stains well, and the result will be a pinkish slide, when it is suppose to look blue-greenish. I had this problem today where I stained an entire rack of slides, and all the slides turned blue (which means they stained correctly) except 4 slides which turned out pinkish (which is a sign that they did not dehydrated properly in sodium chloride (h20 saline). Is there a solution to this issue. Can these slides be re-stained? I know this newsgroup is for histology only but I would like to know if histotech and cytotechs have encountered this issue in their labs and how they ended up solving the problem. Thanks in advance From vrodriguez10 <@t> gmail.com Fri Apr 30 19:48:58 2010 From: vrodriguez10 <@t> gmail.com (Valerie R.) Date: Fri Apr 30 19:49:23 2010 Subject: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems In-Reply-To: References: Message-ID: I forgot to include the complete protocol that my lab uses so you can help me better: 1.After dehydrating in sodium chloride for 25 seconds you have to dip the slides in alcohol 95. 2. 10 dips in water then 1.30 minutes in hematoxylin, it used to be 1 min, then rinse in water. 3. 1 min in rinse solution 4.10 dips in water. 5. 1 min in bluing solution 6. 10 dips in water. 7. 10 dips in alcohol. 8. 10 dips in alcohol. 9. 1 min in orange solution 10. 15 dips in 95 alcohol. 11. 15 dips in 95 alcohol. 12. 3.15 mins in EA solution 13. The slides are then dipped in 3 containers of 95 alcohol (20 dips each) 14. Slides are dipped in 3 containers of 99 alcohol (15 dips in each) 15. Then they are dipped in Isoxylene which is a combination of xylene and 99 alcohol (15 dips) 16. And lastly Xylene 10 dips When the slides do not absorb the h20 saline seems that the slides absorb more EA than hematoxylin. I don't know why. On Fri, Apr 30, 2010 at 5:34 PM, Valerie R. wrote: > I am an HTL but I have to stain pap smears. In the Papanicalou procedure > for fine needle aspirations I have to stain the slides in sodium chloride > first for 25 seconds in total (15 seconds in one container and 10 in another > container). This for me is the trickiest part when staining cytology because > if slides are not well dehydrated in saline then the cells are not going to > absorb the stains well, and the result will be a pinkish slide, when it is > suppose to look blue-greenish. > > I had this problem today where I stained an entire rack of slides, and all > the slides turned blue (which means they stained correctly) except 4 slides > which turned out pinkish (which is a sign that they did not dehydrated > properly in sodium chloride (h20 saline). > > Is there a solution to this issue. Can these slides be re-stained? > > I know this newsgroup is for histology only but I would like to know if > histotech and cytotechs have encountered this issue in their labs and how > they ended up solving the problem. > > Thanks in advance > > >