[Histonet] mercury vs led lights

[Merced M Leiker]

Interesting, Tim! (I could be wrong, but I was pretty sure the said that 
when I questioned him about it!) I just checked the Zeiss website and 
apparently they carry 10 diodes to emit light at a particular frequency, 
including the reds.  Now I need to ask him again why we don't have the red 
LED!  Links below:

<http://zeiss-campus.magnet.fsu.edu/tutorials/colibri/index.html>
<http://www.zeiss.de/colibri>


--On Wednesday, September 09, 2009 11:25 AM -0700 "Morken, Tim" 
<Timothy.Morken <@t> ucsfmedctr.org> wrote:

> Merced wrote:  " I forgot to mention. An LED can be made to excite FITC,
> DAPI, and Cy5  (far-red) fluorophores, but they cannot make them in the
> red (Alexa Fluor  555, Cy3, TRITC, etc.) This is per our Zeiss rep."
>
>
>
> I'm not sure why the Zeiss rep said that. Maybe Zeiss itself does not
> carry the LED's in that range but I have used up to 655nm LED.
>
> See this for details on other LED's for fluorescence work:
>
> http://www.fraensrl.com/images/fraen_fluorochromes.pdf
>
>
>
> Tim Morken
> UCSF Medical Center
> San Francisco, CA
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
> Leiker Sent: Wednesday, September 09, 2009 10:44 AM
> To: Nejat Yilmaz; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] mercury vs led lights
>
> I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5
> (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor
> 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted
> microscope we have LEDs and we also have a mercury lamp to cover the red
> range. The Zeiss switches nicely and very smoothly between the light
> sources during viewing and image acquisition.
>
> Sorry for the highly untechnical description, but that is all I know
> about  it.  :-)
>
> Regards,
> Merced
> --On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker
> <leiker <@t> buffalo.edu> wrote:
>
>> LEDs don't quench your fluorescence as quickly as mercury.
>>
>>
>> --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz
>> <nyilmaz <@t> mersin.edu.tr> wrote:
>>
>>> Dear Colleagues,
>>>
>>> We're attempted to buy a new inverted fluorescence microscope for our
>>> research lab. Distrubitor of leica offered us a new type system working
>>> with led lights which is about same price. Does anybody know advantages
>>> and disadvantages of this system compared to conventional (with mercury
>>> lamp) fluorescence microscopes.
>>> Thanks in advance.
>>>
>>> Necat Yilmaz
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>
>>
>>
>> Merced M Leiker
>> Research Technician II
>> Cardiovascular Medicine
>> 348 Biomedical Research Building
>> State University of New York at Buffalo
>> 3435 Main St, Buffalo, NY 14214  USA
>> leiker <@t> buffalo.edu
>> 716-829-6118 (Ph)
>> 716-829-2665 (Fx)
>>
>> No trees were harmed in the sending of this email.
>> However, many electrons were severely inconvenienced.
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> Merced M Leiker
> Research Technician II
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>



Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.