From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Sep 1 06:09:10 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Sep 1 06:09:15 2009 Subject: [Histonet] FNA SLIDES Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07727383@wahtntex2.waht.swest.nhs.uk> Adequacy is carried out by the Biomedical Scientist usually in the UK. I make a couple of air dried per passage and stain Diff Quick; you can then give an indication of adequacy within 5 min or so. Usually if the FNA is performed properly you don't get much material;; for example breast and LN's are usually sparse and you get maybe a couple of slides (we don't check these for adequacy as the Site is easily re-needled). Pancreas, Thyroid and Lung are another matter. Usually CT orientated FNAC of the lung tend not to be too cellular but sometimes they are; I used to stain a couple for adequacy and make as many slides as possible. The problem is if you make loads of slides and malignant cells are not apparent in those you stain then you have to look at all the others; tough. If there are malignant cells abundant then you ought to look at all the slides just in case they hold diagnostic information. In the end we did 6 slides and washed the remainder into a pot with saline and did Cytospins if appropriate. Pancreatic FNACs taken under a ultra sound flexible scope can also be very cellular (blood) and the above statement holds. Thyroid's can be very bloody, very bloody; trick is not to pull on the plunger but let it seep into the syringe. Too much blood and what little you have gets well diluted!! Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: 31 August 2009 19:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, "masses" etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 <@t> gmail.com Tue Sep 1 07:31:56 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Tue Sep 1 07:32:00 2009 Subject: [Histonet] Endogenous biotin blocking Message-ID: I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally From LSebree <@t> uwhealth.org Tue Sep 1 07:39:40 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Sep 1 07:39:45 2009 Subject: [Histonet] Endogenous biotin blocking In-Reply-To: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5890357068F@UWHC-MAIL01.uwhis.hosp.wisc.edu> We use biotin blocking only with certain tissues, i.e. liver, kidney, GI, or diseases, i.e. oncocytomas. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Tuesday, September 01, 2009 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endogenous biotin blocking I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Tue Sep 1 08:40:08 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Tue Sep 1 08:40:26 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIFJlOiB1bmV2ZW4gYWx0ZXJuYXRpbmcgc2VjdGlvbnMgb24gY3J5b3N0YXQ=?= References: , Message-ID: <200909012140067190095@foxmail.com> that's funny~~ just keep everything tight and unmovable. also, we use anti-roll guide to keep the sections flat so, sometimes there is a bit OCT attached to the blade/glass/anti-roll guide or surrounding the tissue. clean it. have you tried to cut at a higher temperature? the tissue could be too hard ! this always happen if you are using the frozen microtome, but applies to cryostat as well. 2009-09-01 TF ???? Emily Sours ????? 2009-08-28 22:58:24 ???? Johnson, Teri ??? histonet ??? Re: [Histonet] Re: uneven alternating sections on cryostat You've gotten great advice so far, but if it doesn't help--our problem was that the specimen head was loose--even when it was locked, it would move very slightly. This has to be fixed by servicing it, unfortunately. It was caused by our MD/PhD student trying to adjust the specimen head when it was locked, hence loosening the screws. It happened even though I told the guy to quit doing it, which drove me mad. How some people get this far is beyond me. Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Tue Sep 1 08:56:12 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Sep 1 08:56:17 2009 Subject: [Histonet] PIN 4 Message-ID: <231317.16410.qm@web43509.mail.sp1.yahoo.com> I went to archives to look for this but I didn't get a definite answer to my specific question. I am using Biocare's Prostate Cocktail - 2X (CK5 + CK14?+ p63) and Biocare's P504S-2X? on the Ventana?platform using UltraView DAB and Ultra View RED kits. To code this procedure, is it 88342 X 3 or 88342 X 4? I would like some rationale as well. Thanks! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From JWeems <@t> sjha.org Tue Sep 1 09:33:29 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Sep 1 09:33:48 2009 Subject: [Histonet] PIN 4 In-Reply-To: <231317.16410.qm@web43509.mail.sp1.yahoo.com> References: <231317.16410.qm@web43509.mail.sp1.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57C28C5@ITSSSXM01V6.one.ads.che.org> Its three... Two different chromagens, 1 nuclear and two cytoplasmic abs, which cannot be differentiated. You can bill for what can be separately identified. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Tuesday, September 01, 2009 09:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PIN 4 I went to archives to look for this but I didn't get a definite answer to my specific question. I am using Biocare's Prostate Cocktail - 2X (CK5 + CK14?+ p63) and Biocare's P504S-2X? on the Ventana?platform using UltraView DAB and Ultra View RED kits. To code this procedure, is it 88342 X 3 or 88342 X 4? I would like some rationale as well. Thanks! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From NMargaryan <@t> childrensmemorial.org Tue Sep 1 09:54:28 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Sep 1 09:57:22 2009 Subject: [Histonet] acid phosphatase in tissues in routine immunoperoxidase staining? Message-ID: Good morning histonetters, My question is: Would the presence of acid phosphatase in tissues cause non-specific background staining when doing routine immunoperoxidase staining? If answer is "YES": how to block it? Thanks, Naira From aazath <@t> hotmail.com Tue Sep 1 10:48:39 2009 From: aazath <@t> hotmail.com (Aazath Raj) Date: Tue Sep 1 10:48:43 2009 Subject: [Histonet] H&E preparation Message-ID: Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living?learn the ropes on MSN India http://in.msn.com From richardsje <@t> verizon.net Tue Sep 1 11:53:12 2009 From: richardsje <@t> verizon.net (richardsje@verizon.net) Date: Tue Sep 1 11:53:36 2009 Subject: [Histonet] Vitamin D Receptor Staining Message-ID: <688535026.540115.1251823992992.JavaMail.root@vms124.mailsrvcs.net> I have been trying perform immunohistochemisty on formalin-fixed paraffin-embedded skin samples with a vitamin D receptor antibody with no luck. I'm using the Abnova antibody VDR monoclonal, clone 2F4 (catalog #H00007421-M02). Has anyone used this antibody, even on non-skin samples? If so, what was your protocol? Or, if you have used a different mouse VDR antibody, could you let me know how it worked? Thanks! Joanna From carl.hobbs <@t> kcl.ac.uk Tue Sep 1 13:23:46 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Tue Sep 1 13:24:11 2009 Subject: [Histonet] Re:Endogenous biotin blocking Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A1D@KCL-MAIL04.kclad.ds.kcl.ac.uk> Your understanding is correct, imho. I add a "no primary" control on my stABCpx-DAB run whenever I am testing new tissues and assess any positivity that could be due to endogenous biotin. You will quickly build up a knowledge of those tissues that automatically require biotin - blocking. ( eg liver, kidney) If you are a clinical/registered Lab, you wil of course be required to buy a commercial kit. If you are in a research lab and find that you will need to apply biotin blocking regularly, you can make up your own avidin/biotin solutions if costs are a major factor. I will be interested in other suggestions/approaches. carl From katelin <@t> cuttingedgehistology.com Tue Sep 1 13:47:22 2009 From: katelin <@t> cuttingedgehistology.com (Katelin Lester) Date: Tue Sep 1 13:47:28 2009 Subject: [Histonet] Marginated Staining on IHC Message-ID: Hi histonet, I am back with a problem I've been having with my IHC slides. We had a PM recently and had thought that would solve this problem, but as I do more and more slides, the problem remains. I am seeing marginated staining at the edge of the tissue on the controls as well as the patient tissue. This occurs at the top, middle, and bottom of the slides. This occurs with any antibody. This does not occur with every run, but the more slides I have on the stainer, the more frequently it occurs. I have contacted Thermo, who now takes care of our old R.A.S. Microm HMS 710i, but I have further faith that the histonet can help me. I've seen similar posts in the archives, but did not see any responses. Any suggestions are appreciated, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 From rjbuesa <@t> yahoo.com Tue Sep 1 14:22:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 1 14:22:16 2009 Subject: [Histonet] H&E preparation In-Reply-To: Message-ID: <960806.99145.qm@web65709.mail.ac4.yahoo.com> The hematoxylin has to be ripen, either naturally (it takes long time) or with an oxidizer. Ren? J. --- On Tue, 9/1/09, Aazath Raj wrote: From: Aazath Raj Subject: [Histonet] H&E preparation To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 1, 2009, 11:48 AM Dear All, ? ? ? ? ? We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living?learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> exchange.hsc.mb.ca Tue Sep 1 16:14:18 2009 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Tue Sep 1 16:14:24 2009 Subject: [Histonet] Book specific for Neuropathology techniques/methods Message-ID: Hi, Can anyone on the Histonet recommend a good book of Neuropathology techniques/methods for use in a Neuropathology Lab. Need more up to date reference material. Thanks Sharon Allen Neuropathology Lab HSC - Wpg sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From AnthonyH <@t> chw.edu.au Tue Sep 1 19:21:20 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 1 19:21:26 2009 Subject: [Histonet] H&E preparation In-Reply-To: Message-ID: Aazath, Several possibles: It can come down to the ripening time of the haematoxylin. Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!) mercury oxide, added) will tend to stain strongly initially but tend to over-oxidise giving weak or brown nuclear staining. Heating the solution and adding the oxidiser will cause the same thing. Less oxidiser will give you a solution with a longer self life but weaker staining. If the pH is too low, staining will be weaker, but more specific. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: Wednesday, 2 September 2009 1:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E preparation Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living...learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Kim.Osullivan <@t> med.monash.edu.au Tue Sep 1 21:33:52 2009 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Tue Sep 1 21:34:00 2009 Subject: [Histonet] Mast cell staining HELP! Message-ID: <130.194.114.97.1251858518@my.monash.edu.au> Hi, Can anyone out there recommend the best way to stain mouse mast cells in the kidney(toluidine blue/or berberine sulfate) with a protocol that produces cconsistent results? Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys. Does anyone know of a company that supplies antibodies which will work on any of these fixatives,for the staining of the IgE receptor, mast cell trytptase and chymase. Any advice would be appreciated! Kim O'Sullivan Monash University Melbourne Australia From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Sep 2 02:53:46 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Sep 2 02:53:59 2009 Subject: [Histonet] H&E preparation Message-ID: <86ADE4EB583CE64799A9924684A0FBBF077273FD@wahtntex2.waht.swest.nhs.uk> Ripening in the sun (we do get some in the UK) used to give Haematoxylin that was stable; if you forgot to make any up then you hastely added oxidising agent to a new batch or 'jumped started' a batch that was as yet still immature. That batch then never seemed to last as long as you got muddy staining. They looked quite pretty, lines of 5 litres flasks with their contents ripening in the sun with cotton wool balls sticking out of the top, next to the cigarette ashtrays. How things have changed.............. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: 01 September 2009 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E preparation Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living...learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Wed Sep 2 06:01:39 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Sep 2 06:01:54 2009 Subject: [Histonet] H&E preparation Message-ID: <4A9E1853020000EE0002C151@smtp-gw.hurleymc.com> Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric oxide) too soon when it was still bubbling and having a volcano of hematoxylin?!!! LOLj Was remembering tho', that every day, before using, we would "top off" (add...oh, about 30ml) of fresh to the filtered used batch. This really kept the quality good. Worked for 30 yrs until we started purchasing it. Gone are the morning coffee and donuts sitting next to our microtomes too!! Sigh................ Ya, ya....I know!!! >>> "Kemlo Rogerson" 09/02/09 3:53 AM >>> Ripening in the sun (we do get some in the UK) used to give Haematoxylin that was stable; if you forgot to make any up then you hastely added oxidising agent to a new batch or 'jumped started' a batch that was as yet still immature. That batch then never seemed to last as long as you got muddy staining. They looked quite pretty, lines of 5 litres flasks with their contents ripening in the sun with cotton wool balls sticking out of the top, next to the cigarette ashtrays. How things have changed.............. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: 01 September 2009 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E preparation Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living...learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From september.amspacher <@t> bassett.org Wed Sep 2 06:02:30 2009 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Wed Sep 2 06:02:36 2009 Subject: [Histonet] Ink issues Message-ID: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York From Susan.Walzer <@t> HCAHealthcare.com Wed Sep 2 07:18:22 2009 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Wed Sep 2 07:18:31 2009 Subject: [Histonet] RE: Ink issues In-Reply-To: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> References: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AC41FD3CD@FWDCWPMSGCMS09.hca.corpad.net> My Docs use acetone. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed Sep 2 08:10:42 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Sep 2 08:10:51 2009 Subject: [Histonet] Gloves References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE2E@EXCHMBC2.ad.ah.local> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2B1E@fhosxchmb006.ADVENTISTCORP.NET> We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when exposed to Histology chemicals. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Merced M Leiker Sent: Mon 8/31/2009 12:43 PM To: Mahoney,Janice A; 'Histonet' Subject: Re: [Histonet] Gloves I would like to know the answer to this one too. I've been using nitrile gloves, which are rated as having moderate protection against xylene (they slow down how quickly xylene goes through them?) The brand I use is what's in our stockroom: Kimberly Clark and they come in a pretty purple. :-) --On Monday, August 31, 2009 11:15 AM -0500 "Mahoney,Janice A" wrote: > I'd like some advice about gloves. What brand/vendor are people using > that are approved for use with xylene? Thanks, > Jan Mahoney > Omaha > > ________________________________ > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, > Alegent Health is faithful to the healing ministry of Jesus Christ, > providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, > is confidential and private and intended only for the use of the > addressees. Unauthorized use, disclosure, distribution or copying is > strictly prohibited and may be unlawful. If you received this > communication in error, please inform us of the erroneous delivery by > return e-mail message from your computer. Additionally, although all > attachments have been scanned at the source for viruses, the recipient > should check any attachments for the presence of viruses before opening. > Alegent Health accepts no liability for any damage caused by any virus > transmitted by this e-mail. Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Wanda.Smith <@t> HCAhealthcare.com Wed Sep 2 09:07:02 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Sep 2 09:07:13 2009 Subject: [Histonet] RE: Ink issues In-Reply-To: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> References: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BAFC79639B@NADCWPMSGCMS03.hca.corpad.net> Good Morning, We dip the inked tissue in a container of Acetone. We use the 7-dye kit from Bradley Products, Inc. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Sep 2 09:09:55 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 2 09:09:59 2009 Subject: [Histonet] Ink issues In-Reply-To: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> References: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57C2B89@ITSSSXM01V6.one.ads.che.org> We use acetic acid - 3-5% solution (vinegar). j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 07:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JWeems <@t> sjha.org Wed Sep 2 09:14:10 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 2 09:14:36 2009 Subject: [Histonet] H&E preparation In-Reply-To: <4A9E1853020000EE0002C151@smtp-gw.hurleymc.com> References: <4A9E1853020000EE0002C151@smtp-gw.hurleymc.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57C2B8E@ITSSSXM01V6.one.ads.che.org> the good ole days for sure - purple ceilings, purple walls, purple people.... And cigarette ashes in the trash and food on the counter... Is it Friday yet?!!! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, September 02, 2009 07:02 To: aazath@hotmail.com; histonet@lists.utsouthwestern.edu; Kemlo.Rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] H&E preparation Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric oxide) too soon when it was still bubbling and having a volcano of hematoxylin?!!! LOLj Was remembering tho', that every day, before using, we would "top off" (add...oh, about 30ml) of fresh to the filtered used batch. This really kept the quality good. Worked for 30 yrs until we started purchasing it. Gone are the morning coffee and donuts sitting next to our microtomes too!! Sigh................ Ya, ya....I know!!! Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From dchihc <@t> yahoo.com Wed Sep 2 10:26:06 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Sep 2 10:26:11 2009 Subject: [Histonet] Robotic Prostatectomy Message-ID: <21877.6306.qm@web43503.mail.sp1.yahoo.com> For the first time here, we have Urologists?using the robotic method for removing prostates.?The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed.??The?prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. Has anyone had similar experiences? ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From JamesJenniferD <@t> uams.edu Wed Sep 2 10:54:28 2009 From: JamesJenniferD <@t> uams.edu (James, Jennifer) Date: Wed Sep 2 10:55:16 2009 Subject: [Histonet] (no subject) Message-ID: <22AD9C7B0B022246AEF93ECF29BDB0D40B317D617E@MAIL2.ad.uams.edu> Does anyone have experience with the Cell Technology Apo-single stranded DNA antibody? Please let me know if you do. Thanks! Jennifer James BS, HT(ASCP)HTL, QIHC, CRS Research Assistant Experimental Pathology Laboratory Winthrop P.Rockefeller Cancer Institute, Room 429 Universtiy of Arkansas for Medical Sciences 4301 West Markham Street, #725 Little Rock, AR 72205 501-686-8265 jamesjenniferd@uams.edu From AJohnson <@t> aipathology.com Wed Sep 2 11:11:50 2009 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Wed Sep 2 11:11:56 2009 Subject: [Histonet] Cost to produce an H&E slide Message-ID: <704247D5A09D004C9E6B115138D1703A07329E@hpserv001.aipathology.local> Hello Histonet.....Has anyone ever figured out how much it costs to produce an H&E slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson From rjbuesa <@t> yahoo.com Wed Sep 2 11:51:25 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 2 11:52:07 2009 Subject: [Histonet] Robotic Prostatectomy In-Reply-To: <21877.6306.qm@web43503.mail.sp1.yahoo.com> Message-ID: <909608.59241.qm@web65704.mail.ac4.yahoo.com> And your problem will not be solved unless the prostates, after being inked, are cut in half before placing them in the fixative. I do not see any problem in cutting in half a properly inked?prostate to assure proper fixation and subsequent processing. Ren? J.? --- On Wed, 9/2/09, Phyllis Thaxton wrote: From: Phyllis Thaxton Subject: [Histonet] Robotic Prostatectomy To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 2, 2009, 11:26 AM For the first time here, we have Urologists?using the robotic method for removing prostates.?The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed.??The?prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. Has anyone had similar experiences? ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Sep 2 12:24:06 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Sep 2 12:24:10 2009 Subject: [Histonet] Re: Ink issues Message-ID: About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Wed Sep 2 12:34:09 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Sep 2 12:34:14 2009 Subject: [Histonet] Re: Gloves Message-ID: Janet Bonner notes: >We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when exposed to Histology chemicals.<< Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called "chemotherapy gloves" - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN From joseph-galbraith <@t> uiowa.edu Wed Sep 2 12:48:34 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Wed Sep 2 12:48:46 2009 Subject: [Histonet] Re: Ink issues In-Reply-To: References: Message-ID: I concur with the Samurai Pathologist. Long ago, we used Bouin's but have stopped that procedure and prefer to just blot dry but do also use 3% acetic acid, especially if blotting could potentially damage a delicate tissue. Good luck. Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, September 02, 2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ink issues About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed Sep 2 12:53:36 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Sep 2 12:53:51 2009 Subject: [Histonet] Re: Gloves References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2B22@fhosxchmb006.ADVENTISTCORP.NET> It says on the box that these aloe touch nitrile gloves are "Latex-free, powder-free nitrile examination gloves, single use, non-sterile. Tested for use with chemotherapy drugs" We've had a terrible time with getting good gloves, and then when we get some good ones, they are replaced the following year with a cheaper brand until our PAs start screaming. You're right - Surgery wouldn't even have to whistle!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Wed 9/2/2009 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gloves Janet Bonner notes: >We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when exposed to Histology chemicals.<< Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called "chemotherapy gloves" - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 2 13:01:31 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Sep 2 13:02:01 2009 Subject: [Histonet] Re: Ink issues In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4CE1593FCA@EXMBMCB15.ucsfmedicalcenter.org> Maybe it is the acetic acid in Bouin's that is the "active ingredient" for dye adherence! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Wednesday, September 02, 2009 10:49 AM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ink issues I concur with the Samurai Pathologist. Long ago, we used Bouin's but have stopped that procedure and prefer to just blot dry but do also use 3% acetic acid, especially if blotting could potentially damage a delicate tissue. Good luck. Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, September 02, 2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ink issues About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From diane.gladney <@t> us.army.mil Wed Sep 2 13:41:32 2009 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Wed Sep 2 13:41:48 2009 Subject: [Histonet] Ink issues (UNCLASSIFIED) In-Reply-To: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> References: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> Message-ID: <6673D9F600F29943A80ACD92795D345068C9A5@amedsermcbe042.amed.ds.army.mil> Classification: UNCLASSIFIED Caveats: NONE We use "Distilled White Vinegar". I buy it at the grocery store by the gallon. It is the acetic acid in bouin's solution that fixes the ink to the tissue, not the picric acid. I have been using white vinegar for more years than I care to remember. Hope this helps. Diane Gladney Supervisor, Histology Dept. Moncrief Army Community Hospital Ft. Jackson, SC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE From arvidsonkristen <@t> yahoo.com Wed Sep 2 13:44:17 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Sep 2 13:46:14 2009 Subject: [Histonet] Uranyl Nitrate Message-ID: <268995.53619.qm@web65704.mail.ac4.yahoo.com> I am looking for an alternate disposal method for my Uranyl Nitrate.? I was quoted at about $3000 from our haz. waste disposal company.? I would like to find a cheaper method.? I cannot ship it outside the US.? Would anyone be able to take it and add it to their waste?? I have about 500ml of a 1% aq. solution?to get rid of.? Please let me know if this might be a possibility, I will pay for service.? Thank you!! From sheila_adey <@t> hotmail.com Wed Sep 2 13:50:46 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Sep 2 13:50:50 2009 Subject: [Histonet] (no subject) Message-ID: Does anyone have a good recipe for making up a dissect aid in house? Sheila Adey HT MLT _________________________________________________________________ New! Open Messenger faster on the MSN homepage http://go.microsoft.com/?linkid=9677405 From leiker <@t> buffalo.edu Wed Sep 2 13:52:46 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 2 13:52:53 2009 Subject: [Histonet] Re: Gloves In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2B22@fhosxchmb006.ADVENTISTCORP.NET> References: <5F31F38C96781A4FBE3196EBC22D47807F2B22@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <934675BFD8EA82A05633A2AC@CDYwxp1931.ad.med.buffalo.edu> I was just doing some research on the original question posted of gloves suitable for handling xylene. Nitrile is a synthetic rubber made of acrylnitrile and butadiene, devoid of allergy-causing proteins like in latex and natural rubber. The U.S. Dept. of Energy suggests using nitrile when handling xylenes, Ansell suggests either their laminate film gloves, PVA, or nitrile on their very detailed chart offering technical permeabilty data (for instance, it takes 75 min for xylene to break through their nitrile gloves). Now according to OSHA, nitrile does not provide adequate protection, but gives other glove types that are in agreement with what is suggested by some of the other sites I saw. Some of the sites don't seem to say how THICK these gloves are that they are rating, which I think would be a factor for permeability rate. Most of Ansell's gloves were quite THICK (like for industrial use). Another factor for permeability would be how long xylene will be in contact with your glove, if you change gloves frequently or soon after getting xylene on them it seems you'd be ok. I find nitrile (the flexible thin healthcare ones) to be pretty cost-effective. At least, I've dipped my nitrile-gloved fingers in a xylene bath and when I changed my gloves a few minutes later my skin and the inside of the glove was still dry. References: http://www.aps.anl.gov/Safety_and_Training/User_Safety/gloveselection.html http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf http://www.osha.gov/SLTC/healthguidelines/xylene/recognition.html Regards, Merced --On Wednesday, September 02, 2009 1:53 PM -0400 "Bonner, Janet" wrote: > It says on the box that these aloe touch nitrile gloves are "Latex-free, > powder-free nitrile examination gloves, single use, non-sterile. Tested > for use with chemotherapy drugs" We've had a terrible time with > getting good gloves, and then when we get some good ones, they are > replaced the following year with a cheaper brand until our PAs start > screaming. You're right - Surgery wouldn't even have to whistle!! > Janet > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert > Richmond Sent: Wed 9/2/2009 1:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Gloves > > > > Janet Bonner notes: > >> We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 >> for small size) www.medline.com. (1-800 - medline) These gloves >> are not stiff, they fit the hand 'like a glove' . They say on the box >> "not intended to be used as a chemical barrier", but they do a great job >> when exposed to Histology chemicals.<< > > Not familiar with this product, but with nitrile rubber gloves brand > name is important, as the quality of nitrile rubber gloves worsens. > When they first came out and were made in the USA, I could make a pair > last for two weeks of grossing. As manufacture moved to the > latex-producing countries, nitrile rubber (if that's what they > actually are) gloves became no better than latex. At present I use two > pairs of gloves when I gross. > > There's a little-known product called "chemotherapy gloves" - thick > blue latex. I filched a box of these and use them - they last for > several days. Obviously re-using gloves offsets the high initial cost > of purchase, but that's not good MBA thinking. > > OSHA says not to handle formaldehyde (and I suppose xylene) with latex > gloves, but has not specified an alternative. > > It's disgraceful how little the powers that be care about the hands of > pathologists and histotechnologists. You can bet that if it were > nurses who had this problem, it'd get solved in a hurry. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ======================================================= > The information contained in this message may be privileged and/or > confidential and protected from disclosure. If the reader of this > message is not the intended recipient or an employee or agent > responsible for delivering this message to the intended recipient, you > are hereby notified that any dissemination, distribution or copying of > this communication is strictly prohibited. If you have received this > communication in error, please notify the sender immediately by replying > to this message and deleting the material from any computer. > ======================================================= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Lynn.Burton <@t> Illinois.gov Wed Sep 2 14:02:16 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Sep 2 14:04:32 2009 Subject: [Histonet] Cost to produce an H&E slide References: <704247D5A09D004C9E6B115138D1703A07329E@hpserv001.aipathology.local> Message-ID: I don't know what the COST to produce an H&E is but we charge outside entities $8 each to stain them. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amy Johnson Sent: Wed 9/2/2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost to produce an H&E slide Hello Histonet.....Has anyone ever figured out how much it costs to produce an H&E slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab.hymclab <@t> ministryhealth.org Wed Sep 2 14:11:03 2009 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Sep 2 14:15:55 2009 Subject: [Histonet] Re: Gloves In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2B22@fhosxchmb006.ADVENTISTCORP.NET> References: <5F31F38C96781A4FBE3196EBC22D47807F2B22@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: We also use the Aloetouch gloves for all aspects of Histology and have had no issues with them. They fit "like a glove" and are very durable but continue to have good flexability. Luckily Medline is on contract with HPG (our buying group)!!! Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 dawn.schneider@ministryhealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, September 02, 2009 12:54 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Gloves It says on the box that these aloe touch nitrile gloves are "Latex-free, powder-free nitrile examination gloves, single use, non-sterile. Tested for use with chemotherapy drugs" We've had a terrible time with getting good gloves, and then when we get some good ones, they are replaced the following year with a cheaper brand until our PAs start screaming. You're right - Surgery wouldn't even have to whistle!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Wed 9/2/2009 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gloves Janet Bonner notes: >We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when exposed to Histology chemicals.<< Not familiar with this product, but with nitrile rubber gloves brand name is important, as the quality of nitrile rubber gloves worsens. When they first came out and were made in the USA, I could make a pair last for two weeks of grossing. As manufacture moved to the latex-producing countries, nitrile rubber (if that's what they actually are) gloves became no better than latex. At present I use two pairs of gloves when I gross. There's a little-known product called "chemotherapy gloves" - thick blue latex. I filched a box of these and use them - they last for several days. Obviously re-using gloves offsets the high initial cost of purchase, but that's not good MBA thinking. OSHA says not to handle formaldehyde (and I suppose xylene) with latex gloves, but has not specified an alternative. It's disgraceful how little the powers that be care about the hands of pathologists and histotechnologists. You can bet that if it were nurses who had this problem, it'd get solved in a hurry. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From JMyers1 <@t> aol.com Wed Sep 2 14:16:53 2009 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Wed Sep 2 14:17:20 2009 Subject: [Histonet] Endogenous biotin blocking Message-ID: Sally: The simple answer to the question of whether or not biotin blocking is required is ?no?. As you stated in your inquiry, blocking need only be performed when excessive nonspecific staining, attributable to endogenous biotin, is observed. For what its worth, I think that some folks believe biotin blocking is required because they've miscontrued information contained within the College of American Pathologists AP checklist, which states: ?If the laboratory uses an avidin-biotin complex (ABC) detection system...is there a policy that addresses nonspecific false positive staining from endogenous biotin?" As written, this question only implies that an assessment of false positive staining be performed. Therefore, provided that the lab conducts the recommended assessment, documents their results within appropriate policies/procedures, and then incorporates biotin blocking steps where they're needed, it does not have to perform blocking at all times. I'd welcome additonal input if my understanding is incorrect. Regards, Joe Myers, M.S., CT(ASCP) ------------------------------ Message: 9 Date: Tue, 1 Sep 2009 08:31:56 -0400 From: Sally Price Subject: [Histonet] Endogenous biotin blocking To: histonet@lists.utsouthwestern.edu I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally From Barry.R.Rittman <@t> uth.tmc.edu Wed Sep 2 14:17:09 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Sep 2 14:18:30 2009 Subject: [Histonet] Cost to produce an H&E slide In-Reply-To: References: <704247D5A09D004C9E6B115138D1703A07329E@hpserv001.aipathology.local>, Message-ID: <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA6B@UTHCMS1.uthouston.edu> The cost around $12 to produce a regularly stained section. The cost to produce a stained section by an very experienced histotech.......priceless. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn [Lynn.Burton@Illinois.gov] Sent: Wednesday, September 02, 2009 2:02 PM To: Amy Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost to produce an H&E slide I don't know what the COST to produce an H&E is but we charge outside entities $8 each to stain them. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amy Johnson Sent: Wed 9/2/2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost to produce an H&E slide Hello Histonet.....Has anyone ever figured out how much it costs to produce an H&E slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsnwl <@t> neuro.hfh.edu Wed Sep 2 14:21:34 2009 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Wed Sep 2 14:21:38 2009 Subject: [Histonet] pERK IHC Message-ID: <2cfdf8daff6cab737580b37e46381643@neuro.hfh.edu> Does anyone have any suggestions for antibody, protocol, etc., regarding staining FFPE sections with pERK? Any information would be greatly appreciated. Thanks in advance, Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit From wdesalvo.cac <@t> hotmail.com Wed Sep 2 14:23:45 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Sep 2 14:23:49 2009 Subject: [Histonet] Cost to produce an H&E slide In-Reply-To: <704247D5A09D004C9E6B115138D1703A07329E@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703A07329E@hpserv001.aipathology.local> Message-ID: Over the years I have not found any published cost calculations that have addressed individual categories that fluctuate constantly in the department and it seems that there are as many models as there are institutions/companies. In the past I have found if I separate costs into a minimum of 4 categories, I can provide a reasonable calculation for the technical component successfully. Full calculation will require that you have a cost for all shared tasks in the department that contribute to the production of the slide (i.e. accessioning, grossing, embedding, cutting) and be able to allocate a portion of that technical cost to the final slide. I have also been asked to add to the calculation to account for items such as Training/Education for staff, taxes/shipping cost and professional fees. I suggest you communicate with Finance to see what detail is required and to capture all costs for your department. I hope this gets you started. Cost Analysis for H&E: Staining Materials + Labor + Instrumentation + Company Cost Allocation Materials - Reagents, Stains, Slides, Cover slips Each item will need to be calculated to number of units (slides) per ordered unit (i.e. gallon, quart, pint, milliliters). Can take total monthly cost of all staining material and divide by total slides stained per month. Example: Hematoxylin = 1500 slides stained per 620 ml (specific to slide staining instrument) or 2.42 slides / mL; Hematoxylin is ordered by the pint or 473 mL; Cost per pint = $29.80 or $0.063/mL; Cost per slide = .063 mL / 2.42 slides or $0.026 per slide. This is only for the Hematoxylin Labor ? Average salary and benefits for all employees that will perform task. Average labor cost will be divided by time spent to perform task. Example: Average hourly labor cost = $24.00; time spent to perform staining task = 4 hours; number of slides stained in 4 hours = 480 slides; Cost per slide = 24 x 4 / 480 = $0.20 per slide Instrumentation ? Cost to purchase plus the cost of repairs and/or maintenance contract or lease/rental cost per year and then the allocation per your institution amortization schedule. Divide by total number of slides stained per year Example: Cost of instrument = $60,000; Amortized over 7 years = 60,000 / 7 = $8572; Cost of service contract per year = $3,000; Total number of slides stained = 100,000; Cost per slide = 8572 + 3,000 / 100,000 = $0.12 per slide. All instruments used in the process will need to be added to the cost calculation (i.e. cover slip instrument). Company Cost Allocation - This could be a variety of additional cost allocated to your department. Air conditioning, lease rental for building, lights, etc. Acquire monthly or year total from Finance. Eample: Allocated casts assigned= $12,000; 100,00 slides per year; Cost per slide = 12000 / 100000 = $0.012 per slide William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 2 Sep 2009 11:11:50 -0500 > From: AJohnson@aipathology.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cost to produce an H&E slide > > Hello Histonet.....Has anyone ever figured out how much it costs to > produce an H&E slide? Are there any articles out there that would help > to figure this out? > > I realize it may be different for each institution but a ball park > figure would be greatly appreciated. > > Thanks > > Amylin Johnson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live: Make it easier for your friends to see what you?re up to on Facebook. http://windowslive.com/Campaign/SocialNetworking?ocid=PID23285::T:WLMTAGL:ON:WL:en-US:SI_SB_facebook:082009 From anonwums1 <@t> gmail.com Wed Sep 2 16:05:03 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Wed Sep 2 16:05:13 2009 Subject: [Histonet] Re: anti-GFP antibody In-Reply-To: <11D9615B89C10747B1C985966A63D7CA29917429ED@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA29917429ED@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: <858249120909021405h69132681t6cd327706acf34b6@mail.gmail.com> Hi all, Just to update you, I ordered Abcam's ab13970 chicken anti-GFP and tested it out in paraffin embedded sections with HIER for 10 minutes in Dako target retrieval system. I came in with a Dylight 488 secondary from Jackson Immunoresearch at 1 ug / mL. It worked beautifully at 1:500, 1:1000, and 1:2000. I should've titered it even further down. Thanks for all your help, Adam On Thu, Aug 20, 2009 at 1:38 PM, Hobbs, Carl wrote: > > I am sure someone has a cunning combination! > I use Abcam's ab13970 chicken anti GFP in Pwax sections after HIER. > For me, it is very good > Image at Abcam or here : http://www.immunoportal.com/index.php > Good luck! > carl > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Margaret.Perry <@t> sdstate.edu Wed Sep 2 17:00:04 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Sep 2 17:00:09 2009 Subject: [Histonet] Tissue Embedder Message-ID: We are in the market for a new embedder. What do you suggest? I tried searching the archives but only came up with older comments. Vendors please feel free to contact Frank.Qin@sdstate.edu. We may have to go with a used instrument so would like comments on good vendors. From webb3655 <@t> sbcglobal.net Wed Sep 2 20:27:25 2009 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Wed Sep 2 20:27:29 2009 Subject: [Histonet] Dragon dictating system Message-ID: <281799.44322.qm@web83605.mail.sp1.yahoo.com> Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney??? John Peter Smith Hospital Fort Worth Texas 817-927-1024 From webb3655 <@t> sbcglobal.net Wed Sep 2 20:35:34 2009 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Wed Sep 2 20:35:39 2009 Subject: [Histonet] Ink issues Message-ID: <731072.21842.qm@web83604.mail.sp1.yahoo.com> We? blot the tissue dry, ink specimen and dip into 50% vinegar .? Black and green work best, but all colors work. Judy McKinney JPS Hospital Fort Worth Texas From annigyg <@t> gmail.com Thu Sep 3 01:45:39 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Sep 3 01:45:45 2009 Subject: [Histonet] Tissue Embedder In-Reply-To: References: Message-ID: Tec5 Embedding system from Sakura does it for me - ergonomic and left/right handed Annie 2009/9/3 Perry, Margaret > We are in the market for a new embedder. What do you suggest? I tried > searching the archives but only came up with older comments. > Vendors please feel free to contact Frank.Qin@sdstate.edu Frank.Qin@sdstate.edu>. We may have to go with a used instrument so would > like comments on good vendors. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From tgenade <@t> gmail.com Thu Sep 3 05:01:41 2009 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Thu Sep 3 05:01:47 2009 Subject: [Histonet] Re:Endogenous biotin blocking Message-ID: Hello, > From: "Hobbs, Carl" > Subject: [Histonet] Re:Endogenous biotin blocking > If you are in a research lab and find that you will need to apply biotin > blocking regularly, you can make up your own avidin/biotin solutions if > costs are a major factor. To overcome the problem of endogenous biotin I incubated the sections in an avidin solution. The avidin was the avidin-DH form that comes with the Vector Stain ABC kits. I added it to my blocking solution as per the manufactures instructions and incubated it on the sections for 1 hr at room temperature. There after the sections were washed 3x in buffer and then I added biotin to a final concentration of 1 mg/mL to the blocking solution and incubated for 15 minutes at room temperature. After the biotin step the biotinylated probe in blocking solution was added and incubated over night at 4 deg C. This worked very well! Almost all back ground due to the endogenous biotin was quenched. Regards -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From mike <@t> pathview.com Thu Sep 3 05:51:20 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Sep 3 05:51:40 2009 Subject: [Histonet] Dragon dictating system In-Reply-To: <281799.44322.qm@web83605.mail.sp1.yahoo.com> References: <281799.44322.qm@web83605.mail.sp1.yahoo.com> Message-ID: <001801ca2c84$790ab310$6b201930$@com> Judy, my company and I have been experimenting with Dragon since version 4. The current version is 10. The bottom line summary is Yes, it will work if you 1. put a lot of time in it, or 2. use a lot of macros/shortcuts/items of that nature. When it works, it's pretty awesome. If you need more details, please let me know. I'd be happy to share. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of judy webb Sent: Wednesday, September 02, 2009 9:27 PM To: histonet Subject: [Histonet] Dragon dictating system Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney??? John Peter Smith Hospital Fort Worth Texas 817-927-1024 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From princekunlzy <@t> gmail.com Thu Sep 3 07:15:13 2009 From: princekunlzy <@t> gmail.com (Adeluwoye Oluwatosin) Date: Thu Sep 3 07:15:20 2009 Subject: [Histonet] I am glad to come aboard Histonet Message-ID: <786f96610909030515k5c25089bo8fe06d82959ac8c3@mail.gmail.com> Hello, I am glad to come aboard this network of professionals; Pathological sciences. I am delighted 'cos i am sure this lists is providing volume of useful informations to the members. I was re-directed to the histonet web via my search for info on Formaldehyde fixation. Please Barry, I will be glad to receive useful documents as i am working on a project on Formaldehyde fixation, and my line of interest is the effect of varying concentration on tissues, while also considering the optimum fixation duration. Hope to receive this worthwhile, helpful materials tomy work from you Barry,and other list members. Thanks. Tosin. From melissa.mazan <@t> tufts.edu Thu Sep 3 08:18:38 2009 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Thu Sep 3 08:18:45 2009 Subject: [Histonet] DAB and nuclear staining In-Reply-To: <200909021702.n82H2gOq026640@mail-proofpoint-6a> References: <200909021702.n82H2gOq026640@mail-proofpoint-6a> Message-ID: <4A9FC22E.8070308@tufts.edu> Hi all, I have been having a problem with counterstaining for nuclei - I'm looking at mouse lung tissues and staining macrophages with F4/80 (rat anti-mouse). I use a negative serum control and a rat IgG isotype control. I see very clear staining of macrophages - but when I counterstain with hematoxylin or nuclear red, everything becomes muddy and I can no longer discern the DAB staining. Can anyone offer advice? I am using the Vector hematoxylin, and have tried as little as 5 seconds. Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re:Endogenous biotin blocking (Hobbs, Carl) > 2. Marginated Staining on IHC (Katelin Lester) > 3. Re: H&E preparation (Rene J Buesa) > 4. Book specific for Neuropathology techniques/methods (Sharon Allen) > 5. RE: H&E preparation (Tony Henwood) > 6. Mast cell staining HELP! (Kim O'Sullivan) > 7. RE: H&E preparation (Kemlo Rogerson) > 8. RE: H&E preparation (Lynette Pavelich) > 9. Ink issues (Amspacher, September) > 10. RE: Ink issues (Walzer Susan) > 11. RE: Gloves (Bonner, Janet) > 12. RE: Ink issues (Smith Wanda) > 13. RE: Ink issues (Weems, Joyce) > 14. RE: H&E preparation (Weems, Joyce) > 15. Robotic Prostatectomy (Phyllis Thaxton) > 16. (no subject) (James, Jennifer) > 17. Cost to produce an H&E slide (Amy Johnson) > 18. Re: Robotic Prostatectomy (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 1 Sep 2009 19:23:46 +0100 > From: "Hobbs, Carl" > Subject: [Histonet] Re:Endogenous biotin blocking > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <11D9615B89C10747B1C985966A63D7CA2991742A1D@KCL-MAIL04.kclad.ds.kcl.ac.uk> > > Content-Type: text/plain; charset="us-ascii" > > > Your understanding is correct, imho. > I add a "no primary" control on my stABCpx-DAB run whenever I am testing new tissues and assess any positivity that could be due to endogenous biotin. > You will quickly build up a knowledge of those tissues that automatically require biotin - blocking. ( eg liver, kidney) > If you are a clinical/registered Lab, you wil of course be required to buy a commercial kit. > If you are in a research lab and find that you will need to apply biotin blocking regularly, you can make up your own avidin/biotin solutions if costs are a major factor. > I will be interested in other suggestions/approaches. > carl > > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 1 Sep 2009 11:47:22 -0700 > From: "Katelin Lester" > Subject: [Histonet] Marginated Staining on IHC > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi histonet, > > I am back with a problem I've been having with my IHC slides. We had a PM > recently and had thought that would solve this problem, but as I do more and > more slides, the problem remains. I am seeing marginated staining at the > edge of the tissue on the controls as well as the patient tissue. This > occurs at the top, middle, and bottom of the slides. This occurs with any > antibody. This does not occur with every run, but the more slides I have on > the stainer, the more frequently it occurs. I have contacted Thermo, who > now takes care of our old R.A.S. Microm HMS 710i, but I have further faith > that the histonet can help me. I've seen similar posts in the archives, but > did not see any responses. > > Any suggestions are appreciated, > > Katelin > > > > Katelin Lester > > Cutting Edge Histology Services, LLC > > (503) 443-2157 > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 1 Sep 2009 12:22:06 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] H&E preparation > To: histonet@lists.utsouthwestern.edu, Aazath Raj > Message-ID: <960806.99145.qm@web65709.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > The hematoxylin has to be ripen, either naturally (it takes long time) or with an oxidizer. > Ren?? J. > > --- On Tue, 9/1/09, Aazath Raj wrote: > > > From: Aazath Raj > Subject: [Histonet] H&E preparation > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, September 1, 2009, 11:48 AM > > > > Dear All, > ? ? ? ? ? We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . > > > Aazath > Technical Officer > Apollo Hospitals > India > > _________________________________________________________________ > News, sports, entertainment and fine living???learn the ropes on MSN India > http://in.msn.com_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 1 Sep 2009 16:14:18 -0500 > From: "Sharon Allen" > Subject: [Histonet] Book specific for Neuropathology > techniques/methods > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi, > Can anyone on the Histonet recommend a good book of Neuropathology > techniques/methods for use in a Neuropathology Lab. Need more up to > date reference material. > Thanks > Sharon Allen > Neuropathology Lab > HSC - Wpg > sallen@hsc.mb.ca > -------------- next part -------------- > This email and/or any documents in this transmission is intended for the > addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. > > Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. > > ------------------------------ > > Message: 5 > Date: Wed, 2 Sep 2009 10:21:20 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] H&E preparation > To: "Aazath Raj" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Aazath, > > Several possibles: > It can come down to the ripening time of the haematoxylin. > Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!) > mercury oxide, added) will tend to stain strongly initially but tend to > over-oxidise giving weak or brown nuclear staining. > > Heating the solution and adding the oxidiser will cause the same thing. > > Less oxidiser will give you a solution with a longer self life but > weaker staining. > > If the pH is too low, staining will be weaker, but more specific. > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath > Raj > Sent: Wednesday, 2 September 2009 1:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E preparation > > > > Dear All, > We are preparing harris heamtoxylin for H&E in our laboratory. > some time we get intense nuclear staining some time light weak.Does > anybody know how to control a consistency in the strength . > > > Aazath > Technical Officer > Apollo Hospitals > India > > _________________________________________________________________ > News, sports, entertainment and fine living...learn the ropes on MSN > India http://in.msn.com_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 6 > Date: Wed, 02 Sep 2009 12:33:52 +1000 > From: Kim O'Sullivan > Subject: [Histonet] Mast cell staining HELP! > To: histonet@lists.utsouthwestern.edu > Message-ID: <130.194.114.97.1251858518@my.monash.edu.au> > Content-Type: text/plain; charset=UTF-8 > > Hi, > > Can anyone out there recommend the best way to stain mouse mast cells in the kidney(toluidine blue/or berberine sulfate) with a protocol that produces cconsistent results? > > Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys. Does anyone know of a company that supplies antibodies which will work on any of these fixatives,for the staining of the IgE receptor, mast cell trytptase and chymase. > > Any advice would be appreciated! > > > Kim O'Sullivan > Monash University > Melbourne > Australia > > > > ------------------------------ > > Message: 7 > Date: Wed, 2 Sep 2009 08:53:46 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] H&E preparation > To: "Aazath Raj" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF077273FD@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Ripening in the sun (we do get some in the UK) used to give Haematoxylin > that was stable; if you forgot to make any up then you hastely added > oxidising agent to a new batch or 'jumped started' a batch that was as > yet still immature. That batch then never seemed to last as long as you > got muddy staining. They looked quite pretty, lines of 5 litres flasks > with their contents ripening in the sun with cotton wool balls sticking > out of the top, next to the cigarette ashtrays. How things have > changed.............. > > > > > > > > > Kemlo Rogerson > e-mail kemlorogerson@nhs.net if not at work. > DD 01934 647057 or extension 3311 Mob 07749 754194; > Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah > Lehrer > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath > Raj > Sent: 01 September 2009 16:49 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E preparation > > > Dear All, > We are preparing harris heamtoxylin for H&E in our laboratory. > some time we get intense nuclear staining some time light weak.Does > anybody know how to control a consistency in the strength . > > > Aazath > Technical Officer > Apollo Hospitals > India > > _________________________________________________________________ > News, sports, entertainment and fine living...learn the ropes on MSN > India http://in.msn.com_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Wed, 02 Sep 2009 07:01:39 -0400 > From: "Lynette Pavelich" > Subject: RE: [Histonet] H&E preparation > To: ,, > > Message-ID: <4A9E1853020000EE0002C151@smtp-gw.hurleymc.com> > Content-Type: text/plain; charset=US-ASCII > > Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric > oxide) too soon when it was still bubbling and having a volcano of > hematoxylin?!!! LOLj > Was remembering tho', that every day, before using, we would "top off" > (add...oh, about 30ml) of fresh to the filtered used batch. This really > kept the quality good. Worked for 30 yrs until we started purchasing > it. Gone are the morning coffee and donuts sitting next to our > microtomes too!! Sigh................ Ya, ya....I know!!! > > >>>> "Kemlo Rogerson" 09/02/09 3:53 AM >>>> >>>> > Ripening in the sun (we do get some in the UK) used to give Haematoxylin > that was stable; if you forgot to make any up then you hastely added > oxidising agent to a new batch or 'jumped started' a batch that was as > yet still immature. That batch then never seemed to last as long as you > got muddy staining. They looked quite pretty, lines of 5 litres flasks > with their contents ripening in the sun with cotton wool balls sticking > out of the top, next to the cigarette ashtrays. How things have > changed.............. > > > > > > > > > Kemlo Rogerson > e-mail kemlorogerson@nhs.net if not at work. > DD 01934 647057 or extension 3311 Mob 07749 754194; > Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah > Lehrer > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aazath > Raj > Sent: 01 September 2009 16:49 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E preparation > > > Dear All, > We are preparing harris heamtoxylin for H&E in our laboratory. > some time we get intense nuclear staining some time light weak.Does > anybody know how to control a consistency in the strength . > > > Aazath > Technical Officer > Apollo Hospitals > India > > _________________________________________________________________ > News, sports, entertainment and fine living...learn the ropes on MSN > India http://in.msn.com_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Wed, 2 Sep 2009 07:02:30 -0400 > From: "Amspacher, September" > Subject: [Histonet] Ink issues > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> > Content-Type: text/plain; charset="iso-8859-1" > > Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million > > September Amspacher HT(ASCP) > Technical Specialist- Histology Department > Laboratory Chemical Hygiene Officer > Bassett Healthcare > Cooperstown, New York > > > > > > ------------------------------ > > Message: 10 > Date: Wed, 2 Sep 2009 07:18:22 -0500 > From: Walzer Susan > Subject: [Histonet] RE: Ink issues > To: "Amspacher, September" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4BF03F5404EBDE409AF9232DA74B9DED2AC41FD3CD@FWDCWPMSGCMS09.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > My Docs use acetone. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amspacher, September > Sent: Wednesday, September 02, 2009 7:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ink issues > > Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million > > September Amspacher HT(ASCP) > Technical Specialist- Histology Department > Laboratory Chemical Hygiene Officer > Bassett Healthcare > Cooperstown, New York > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Wed, 2 Sep 2009 09:10:42 -0400 > From: "Bonner, Janet" > Subject: RE: [Histonet] Gloves > To: "Merced M Leiker" , "Mahoney,Janice A" > , "Histonet" > > Message-ID: > <5F31F38C96781A4FBE3196EBC22D47807F2B1E@fhosxchmb006.ADVENTISTCORP.NET> > > Content-Type: text/plain; charset=iso-8859-1 > > We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when exposed to Histology chemicals. > > Janet > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Merced M Leiker > Sent: Mon 8/31/2009 12:43 PM > To: Mahoney,Janice A; 'Histonet' > Subject: Re: [Histonet] Gloves > > > > I would like to know the answer to this one too. I've been using nitrile > gloves, which are rated as having moderate protection against xylene (they > slow down how quickly xylene goes through them?) The brand I use is what's > in our stockroom: Kimberly Clark and they come in a pretty purple. :-) > > > --On Monday, August 31, 2009 11:15 AM -0500 "Mahoney,Janice A" > wrote: > > >> I'd like some advice about gloves. What brand/vendor are people using >> that are approved for use with xylene? Thanks, >> Jan Mahoney >> Omaha >> >> ________________________________ >> Sponsored by Catholic Health Initiatives and Immanuel Health Systems, >> Alegent Health is faithful to the healing ministry of Jesus Christ, >> providing high quality care for the body, mind and spirit of every person. >> >> The information contained in this communication, including attachments, >> is confidential and private and intended only for the use of the >> addressees. Unauthorized use, disclosure, distribution or copying is >> strictly prohibited and may be unlawful. If you received this >> communication in error, please inform us of the erroneous delivery by >> return e-mail message from your computer. Additionally, although all >> attachments have been scanned at the source for viruses, the recipient >> should check any attachments for the presence of viruses before opening. >> Alegent Health accepts no liability for any damage caused by any virus >> transmitted by this e-mail. Thank you for your cooperation. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ======================================================= > The information contained in this message may be privileged and/or confidential > and protected from disclosure. If the reader of this message is not the intended > recipient or an employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any dissemination, distribution > or copying of this communication is strictly prohibited. If you have received this > communication in error, please notify the sender immediately by replying to this > message and deleting the material from any computer. > ======================================================= > > ------------------------------ > > Message: 12 > Date: Wed, 2 Sep 2009 09:07:02 -0500 > From: Smith Wanda > Subject: [Histonet] RE: Ink issues > To: "Amspacher, September" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <9E2D36CE2D7CBA4A94D9B22E8328A3BAFC79639B@NADCWPMSGCMS03.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > We dip the inked tissue in a container of Acetone. We use the 7-dye kit from Bradley Products, Inc. > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amspacher, September > Sent: Wednesday, September 02, 2009 7:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ink issues > > Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million > > September Amspacher HT(ASCP) > Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 13 > Date: Wed, 2 Sep 2009 10:09:55 -0400 > From: "Weems, Joyce" > Subject: RE: [Histonet] Ink issues > To: > Message-ID: > <5D64396A0D4A5346BEBC759022AAEAA57C2B89@ITSSSXM01V6.one.ads.che.org> > Content-Type: text/plain; charset="us-ascii" > > We use acetic acid - 3-5% solution (vinegar). j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Amspacher, September > Sent: Wednesday, September 02, 2009 07:03 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ink issues > > Good Morning all- I am having issues getting ink to "stick" to the > tissue surface, the black and blue that we are using are fine we have > tried several different companies and even use a "ink stay" acetic acid > spray on the tissue. My new pathologist has talked about dipping the > tissue after it is inked into Bouins solution, and that they didn't have > any issues with inks where he came from. I have heard of this technique > before, But I was just able to get rid of all of the bounins solutions > in the lab and I am hesitant to bring it back, looking for Ideas and > thought from all, also if you use the Bouins solution to "fix" your > inks- I would love to learn your thoughts about the process. > Thanks-a-million > > September Amspacher HT(ASCP) > Technical Specialist- Histology Department Laboratory Chemical Hygiene > Officer Bassett Healthcare Cooperstown, New York > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > > > ------------------------------ > > Message: 14 > Date: Wed, 2 Sep 2009 10:14:10 -0400 > From: "Weems, Joyce" > Subject: RE: [Histonet] H&E preparation > To: > Message-ID: > <5D64396A0D4A5346BEBC759022AAEAA57C2B8E@ITSSSXM01V6.one.ads.che.org> > Content-Type: text/plain; charset="us-ascii" > > > the good ole days for sure - purple ceilings, purple walls, purple > people.... And cigarette ashes in the trash and food on the counter... > > Is it Friday yet?!!! > J:>) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette > Pavelich > Sent: Wednesday, September 02, 2009 07:02 > To: aazath@hotmail.com; histonet@lists.utsouthwestern.edu; > Kemlo.Rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] H&E preparation > > Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric > oxide) too soon when it was still bubbling and having a volcano of > hematoxylin?!!! LOLj Was remembering tho', that every day, before > using, we would "top off" > (add...oh, about 30ml) of fresh to the filtered used batch. This really > kept the quality good. Worked for 30 yrs until we started purchasing > it. Gone are the morning coffee and donuts sitting next to our > microtomes too!! Sigh................ Ya, ya....I know!!! > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > > > ------------------------------ > > Message: 15 > Date: Wed, 2 Sep 2009 08:26:06 -0700 (PDT) > From: Phyllis Thaxton > Subject: [Histonet] Robotic Prostatectomy > To: histonet@lists.utsouthwestern.edu > Message-ID: <21877.6306.qm@web43503.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. > > Has anyone had similar experiences? > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > ------------------------------ > > Message: 16 > Date: Wed, 2 Sep 2009 10:54:28 -0500 > From: "James, Jennifer" > Subject: [Histonet] (no subject) > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <22AD9C7B0B022246AEF93ECF29BDB0D40B317D617E@MAIL2.ad.uams.edu> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have experience with the Cell Technology Apo-single stranded DNA antibody? Please let me know if you do. > Thanks! > > Jennifer James BS, HT(ASCP)HTL, QIHC, CRS > Research Assistant > Experimental Pathology Laboratory > Winthrop P.Rockefeller Cancer Institute, Room 429 > Universtiy of Arkansas for Medical Sciences > 4301 West Markham Street, #725 > Little Rock, AR 72205 > 501-686-8265 > jamesjenniferd@uams.edu > > > > > ------------------------------ > > Message: 17 > Date: Wed, 2 Sep 2009 11:11:50 -0500 > From: "Amy Johnson" > Subject: [Histonet] Cost to produce an H&E slide > To: > Message-ID: > <704247D5A09D004C9E6B115138D1703A07329E@hpserv001.aipathology.local> > Content-Type: text/plain; charset="US-ASCII" > > Hello Histonet.....Has anyone ever figured out how much it costs to > produce an H&E slide? Are there any articles out there that would help > to figure this out? > > I realize it may be different for each institution but a ball park > figure would be greatly appreciated. > > Thanks > > Amylin Johnson > > > > ------------------------------ > > Message: 18 > Date: Wed, 2 Sep 2009 09:51:25 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Robotic Prostatectomy > To: histonet@lists.utsouthwestern.edu, Phyllis Thaxton > > Message-ID: <909608.59241.qm@web65704.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > And your problem will not be solved unless the prostates, after being inked, are cut in half before placing them in the fixative. I do not see any problem in cutting in half a properly inked prostate to assure proper fixation and subsequent processing. > Ren? J. > > --- On Wed, 9/2/09, Phyllis Thaxton wrote: > > > From: Phyllis Thaxton > Subject: [Histonet] Robotic Prostatectomy > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, September 2, 2009, 11:26 AM > > > For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. > > Has anyone had similar experiences? > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 70, Issue 2 > *************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu From mpence <@t> grhs.net Thu Sep 3 08:25:39 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Sep 3 08:25:43 2009 Subject: [Histonet] Tissue Embedder In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BFE@is-e2k3.grhs.net> I was put in the position of having to buy a new embedding center last summer; ours had died and the backup embedder had died also. I had always before had a single unit with cooling plate and wax chamber in one and loved them. Will we were in the position to buy fast and I called all the major vendors. Whom ever could get me a unit ASAP had the sale. Only one vendor came thru and that was the vendor for Tissue-Tek. I had a two piece unit here in two days! I had used the older Tissue-Tek models and chased the modules around the countertop before trying to embed. I though will here goes again. I have to admit I can be very hard to please and vendors do not like to deal with me on instruments. We love our Tissue-Tek embedding center and I am glad to have given Tissue-Tek another chance. (No I am not receiving any monetary compensation for this piece) Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Thursday, September 03, 2009 1:46 AM To: Perry, Margaret Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Embedder Tec5 Embedding system from Sakura does it for me - ergonomic and left/right handed Annie 2009/9/3 Perry, Margaret > We are in the market for a new embedder. What do you suggest? I tried > searching the archives but only came up with older comments. Vendors > please feel free to contact Frank.Qin@sdstate.edu Frank.Qin@sdstate.edu>. We may have to go with a used instrument so > would like comments on good vendors. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 3 08:34:24 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 3 08:34:28 2009 Subject: [Histonet] I am glad to come aboard Histonet In-Reply-To: <786f96610909030515k5c25089bo8fe06d82959ac8c3@mail.gmail.com> Message-ID: <787029.2706.qm@web65708.mail.ac4.yahoo.com> Under separate cover I am sending an article on the subject Ren? J. (not Barry!) --- On Thu, 9/3/09, Adeluwoye Oluwatosin wrote: From: Adeluwoye Oluwatosin Subject: [Histonet] I am glad to come aboard Histonet To: histonet@lists.utsouthwestern.edu Date: Thursday, September 3, 2009, 8:15 AM Hello, I am glad to come aboard this network of professionals; Pathological sciences. I am delighted 'cos i am sure this lists is providing volume of useful informations to the members. I was re-directed to the histonet web via my search for info on Formaldehyde fixation. Please Barry, I will be glad to receive useful documents as i am working on a project on Formaldehyde fixation, and my line of interest is the effect of varying concentration on tissues, while also considering the? optimum fixation duration. Hope to receive this worthwhile, helpful materials tomy work from you Barry,and other list members. Thanks. Tosin. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Sep 3 08:47:31 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Sep 3 08:47:37 2009 Subject: [Histonet] Dragon Message-ID: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E2075@HPEMX3.HealthPartners.int> Our pathology department has been using Dragon for 3 years now and really like it, both PA's and pathologists alike! It is time saving and easy to adapt to, even the Pathologists who have their foreign accents!! In fact, our entire facility is switching to Dragon for dictation. The only negative was the loss of FTE's for secretaries, as it took their work away!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From leiker <@t> buffalo.edu Thu Sep 3 09:02:10 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Sep 3 09:02:15 2009 Subject: [Histonet] I am glad to come aboard Histonet In-Reply-To: <786f96610909030515k5c25089bo8fe06d82959ac8c3@mail.gmail.com> References: <786f96610909030515k5c25089bo8fe06d82959ac8c3@mail.gmail.com> Message-ID: <53006086D12706641A00B8EF@CDYwxp1931.ad.med.buffalo.edu> That is great, Tosin! You are working on a topic that has been discussed, debated, and fought over for years. It means there is a LOT of info out there on it. Start with doing a search for "formaldehyde," "paraformaldehyde," and/or "formalin" in the Histonet Archives. You'll probably find more than you ever wanted to know. :-) Regards, Merced --On Thursday, September 03, 2009 5:15 AM -0700 Adeluwoye Oluwatosin wrote: > Hello, I am glad to come aboard this network of professionals; > Pathological sciences. I am delighted 'cos i am sure this lists is > providing volume of useful informations to the members. I was > re-directed to the histonet web via my search for info on Formaldehyde > fixation. > Please Barry, I will be glad to receive useful documents as i am > working on a project on Formaldehyde fixation, and my line of interest > is the effect of varying concentration on tissues, while also > considering the optimum fixation duration. > Hope to receive this worthwhile, helpful materials tomy work from you > Barry,and other list members. Thanks. > Tosin. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From leiker <@t> buffalo.edu Thu Sep 3 09:02:44 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Sep 3 09:02:49 2009 Subject: [Histonet] I am glad to come aboard Histonet In-Reply-To: <786f96610909030515k5c25089bo8fe06d82959ac8c3@mail.gmail.com> References: <786f96610909030515k5c25089bo8fe06d82959ac8c3@mail.gmail.com> Message-ID: <5DD5F66128AABC77C0671028@CDYwxp1931.ad.med.buffalo.edu> I forgot to include the link: http://www.histosearch.com/histonet.html --On Thursday, September 03, 2009 5:15 AM -0700 Adeluwoye Oluwatosin wrote: > Hello, I am glad to come aboard this network of professionals; > Pathological sciences. I am delighted 'cos i am sure this lists is > providing volume of useful informations to the members. I was > re-directed to the histonet web via my search for info on Formaldehyde > fixation. > Please Barry, I will be glad to receive useful documents as i am > working on a project on Formaldehyde fixation, and my line of interest > is the effect of varying concentration on tissues, while also > considering the optimum fixation duration. > Hope to receive this worthwhile, helpful materials tomy work from you > Barry,and other list members. Thanks. > Tosin. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From abijag76 <@t> yahoo.co.in Thu Sep 3 09:48:38 2009 From: abijag76 <@t> yahoo.co.in (abi jag) Date: Thu Sep 3 09:48:43 2009 Subject: [Histonet] Your expert advice please_histology systems Message-ID: <582158.68197.qm@web95116.mail.in2.yahoo.com> Dear Histonetters, We have planned to procure one set of histology systems for our laboratory(We have the existing one set of instruments all from Leica). We are considering the purchase of Tissue-Tek? VIP? 6(sakura) for tissue processing and Robot-Stainer HMS 740(from microm). Any of our members can share their experience with these instruments to me to enable us for finalizing. Thanks a lot for being in this team. Abijag abijag76@yahoo.co.in See the Web's breaking stories, chosen by people like you. Check out Yahoo! Buzz. http://in.buzz.yahoo.com/ From gu.lang <@t> gmx.at Thu Sep 3 10:05:29 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 3 10:05:37 2009 Subject: AW: [Histonet] Ink issues In-Reply-To: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> References: <71A87F8FEFFB024DB88B064543C7F24B13283139@ex2.bassett.org> Message-ID: <63465BB1835140BF877F98C4A2606003@dielangs.at> We blot the surface dry, mark it with ink and dry it again with a hairdryer for half a minute. -no chemicals Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amspacher, September Gesendet: Mittwoch, 02. September 2009 13:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ink issues Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rebecca.Riesen <@t> nchmd.org Thu Sep 3 10:44:02 2009 From: Rebecca.Riesen <@t> nchmd.org (Riesen, Rebecca) Date: Thu Sep 3 10:44:10 2009 Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 In-Reply-To: Message-ID: Judy, We have been using Dragon also for about the same length of time mentioned by Michael. All pathologists, cytotechs and PA's use it. It is a fantastic program if used properly and with the right attitude. Facts: 1) There are some words that it will always have problems with ie. Tan/ten/tin/ for example are difficult to distinguish between. 2) If in your mind it's not going to work - It won't! It knows :) 3) Creation of Macros, templates and commands make it a breeze. 4) Removing words from its dictionary. Words that you will never ever use can be very helpful when Dragon interprets words with something bizarre. 5) Or the opposite: train a command with a bizarre word ie. "BINGO" so that a command won't be performed in error by speaking a word that could be found in routine dictation. 6) Train it for phrases, rather than single words As you can see there are a lot of little "tricks" that we would be happy to pass on that will make the transition less frustrating. Use other's experiences to expedite your process and I think you too will find it's awesome. Message: 22 Date: Thu, 3 Sep 2009 06:51:20 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Dragon dictating system To: "'judy webb'" , "'histonet'" Message-ID: <001801ca2c84$790ab310$6b201930$@com> Content-Type: text/plain; charset="iso-8859-1" Judy, my company and I have been experimenting with Dragon since version 4. The current version is 10. The bottom line summary is Yes, it will work if you 1. put a lot of time in it, or 2. use a lot of macros/shortcuts/items of that nature. When it works, it's pretty awesome. If you need more details, please let me know. I'd be happy to share. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of judy webb Sent: Wednesday, September 02, 2009 9:27 PM To: histonet Subject: [Histonet] Dragon dictating system Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney John Peter Smith Hospital Fort Worth Texas 817-927-1024 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. From jkiernan <@t> uwo.ca Thu Sep 3 10:49:08 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Sep 3 10:49:13 2009 Subject: [Histonet] Mast cell staining HELP! Message-ID: Mast cells are not conspicuous items in sections of kidneys. In rats and mice mast cell granules are preserved by ordinary formaldehyde fixatives and also by non-aqueous liquids such as Carnoy and "alcoholic Bouin". The granules contain heparin, which can be stained by any cationic dye at low pH. Alcian blue at pH 1.0 does this well. Blue cationic dyes with smaller molecules (thionine, azures, toluidine blue etc) stain heparin metachromatically (red), which is often more conspicuous than the turquoise colour of alcian blue. Technical details can be found in any histological techniques book published since the introduction of alcian blue (1950). Older books have instructions for metachromatic staining of mast cells, which was described in1878: Ehrlich P. Contributions to the Theory and Practice of Histological Staining. (Translated from "Beitr?ge z?r Theorie und Praxis der histologischen F?rbung." University of Leipzig, 1878 (Thesis). In The Collected Papers of Paul Ehrlich, ed. F. Himmelweit & M. Marquandt. Vol 1, pp. 65-98. London & New York: Pergamon Press (1956). John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Kim O'Sullivan Date: Tuesday, September 1, 2009 23:02 Subject: [Histonet] Mast cell staining HELP! To: histonet@lists.utsouthwestern.edu > Hi, > > Can anyone out there recommend the best way to stain mouse mast > cells in the kidney(toluidine blue/or berberine sulfate) with a > protocol that produces cconsistent results? > > Secondly, I have mouse kidney sections fixed in FFPE, PLP and > methyl Carnoys. Does anyone know of a company that supplies > antibodies which will work on any of these fixatives,for > the staining of the IgE receptor, mast cell trytptase and chymase. > > Any advice would be appreciated! > > > Kim O'Sullivan > Monash University > Melbourne > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Sep 3 10:57:54 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 3 10:57:59 2009 Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57C2E84@ITSSSXM01V6.one.ads.che.org> For those who have a positive experience, would you please tell us what LIS you are using? We have partial success with it. We have GE Ultra (formerly Triple G). Some of our docs have issues with the program locking up and having to completely shut down the computer several times a day, so they gave up in frustration. They are the most verbose, of course. We have just tested a patch and will be installing it soon. Maybe that will help. Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Thursday, September 03, 2009 11:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 Judy, We have been using Dragon also for about the same length of time mentioned by Michael. All pathologists, cytotechs and PA's use it. It is a fantastic program if used properly and with the right attitude. Facts: 1) There are some words that it will always have problems with ie. Tan/ten/tin/ for example are difficult to distinguish between. 2) If in your mind it's not going to work - It won't! It knows :) 3) Creation of Macros, templates and commands make it a breeze. 4) Removing words from its dictionary. Words that you will never ever use can be very helpful when Dragon interprets words with something bizarre. 5) Or the opposite: train a command with a bizarre word ie. "BINGO" so that a command won't be performed in error by speaking a word that could be found in routine dictation. 6) Train it for phrases, rather than single words As you can see there are a lot of little "tricks" that we would be happy to pass on that will make the transition less frustrating. Use other's experiences to expedite your process and I think you too will find it's awesome. Message: 22 Date: Thu, 3 Sep 2009 06:51:20 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Dragon dictating system To: "'judy webb'" , "'histonet'" Message-ID: <001801ca2c84$790ab310$6b201930$@com> Content-Type: text/plain; charset="iso-8859-1" Judy, my company and I have been experimenting with Dragon since version 4. The current version is 10. The bottom line summary is Yes, it will work if you 1. put a lot of time in it, or 2. use a lot of macros/shortcuts/items of that nature. When it works, it's pretty awesome. If you need more details, please let me know. I'd be happy to share. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of judy webb Sent: Wednesday, September 02, 2009 9:27 PM To: histonet Subject: [Histonet] Dragon dictating system Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney John Peter Smith Hospital Fort Worth Texas 817-927-1024 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mike <@t> pathview.com Thu Sep 3 11:09:50 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Sep 3 11:10:11 2009 Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 In-Reply-To: References: Message-ID: <006801ca2cb0$f7884690$e698d3b0$@com> Rebecca, that was great advice. You covered a lot of good detail there. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Thursday, September 03, 2009 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 Judy, We have been using Dragon also for about the same length of time mentioned by Michael. All pathologists, cytotechs and PA's use it. It is a fantastic program if used properly and with the right attitude. Facts: 1) There are some words that it will always have problems with ie. Tan/ten/tin/ for example are difficult to distinguish between. 2) If in your mind it's not going to work - It won't! It knows :) 3) Creation of Macros, templates and commands make it a breeze. 4) Removing words from its dictionary. Words that you will never ever use can be very helpful when Dragon interprets words with something bizarre. 5) Or the opposite: train a command with a bizarre word ie. "BINGO" so that a command won't be performed in error by speaking a word that could be found in routine dictation. 6) Train it for phrases, rather than single words As you can see there are a lot of little "tricks" that we would be happy to pass on that will make the transition less frustrating. Use other's experiences to expedite your process and I think you too will find it's awesome. Message: 22 Date: Thu, 3 Sep 2009 06:51:20 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Dragon dictating system To: "'judy webb'" , "'histonet'" Message-ID: <001801ca2c84$790ab310$6b201930$@com> Content-Type: text/plain; charset="iso-8859-1" Judy, my company and I have been experimenting with Dragon since version 4. The current version is 10. The bottom line summary is Yes, it will work if you 1. put a lot of time in it, or 2. use a lot of macros/shortcuts/items of that nature. When it works, it's pretty awesome. If you need more details, please let me know. I'd be happy to share. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of judy webb Sent: Wednesday, September 02, 2009 9:27 PM To: histonet Subject: [Histonet] Dragon dictating system Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney John Peter Smith Hospital Fort Worth Texas 817-927-1024 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Sep 3 12:08:12 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Sep 3 12:08:17 2009 Subject: [Histonet] Book specific for Neuropathology techniques/methods Message-ID: A modern text is Dawson TP, Neal JW, Llewellyn L (2003) Neuropathology Techniques. Arnold, London. The more traditional staining methods are well covered in Ralis HM, Beesley RA, Ralis ZA (1973) Techniques in Neurohistology. Butterworths, London. If you want to use some really tradtitional stains, try Turner OA (1940) A Manual of Neurohistologic Technique. Mosby, St Louis. A nicely illustrated account of necropsy techniques for the nervous system is Adams JH, Murray MF (1982) Atlas of Post-mortem Techniques in Neuropathology. Cambridge, Cambridge University Press. For anatomical dissection of the brain (not usually done by pathologists) the best book is Montemurro DG, Bruni JE (1988) The Human Brain in Dissection, 2nd ed. New York: Oxford University Press. Another interesting read is a paper by EB Jamieson (1909) The means of displaying, by ordinary dissection, the larger tracts of white matter of the brain in their continuity. J. Anat. Physiol. 43: 225-234. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Sharon Allen Date: Tuesday, September 1, 2009 17:15 Subject: [Histonet] Book specific for Neuropathology techniques/methods To: histonet@pathology.swmed.edu > Hi, > Can anyone on the Histonet recommend a good book of Neuropathology > techniques/methods for use in a Neuropathology Lab. Need > more up to > date reference material. > Thanks > Sharon Allen > Neuropathology Lab > HSC - Wpg > sallen@hsc.mb.ca > This email and/or any documents in this transmission is intended > for the > addressee(s) only and may contain legally privileged or > confidential information. Any unauthorized use, > disclosure, distribution, copying or dissemination is strictly > prohibited. If you receive this transmission in error, > please notify the sender immediately and return the original. > > Ce courriel et tout document dans cette transmission est destin? > ? la personne ou aux personnes ? qui il est adress?. Il peut > contenir des informations privil?gi?es ou confidentielles. Toute > utilisation, divulgation, distribution, copie, ou diffusion non > autoris?e est strictement d?fendue. Si vous n'?tes pas le > destinataire de ce message, veuillez en informer l'exp?diteur > imm?diatement et lui remettre l'original.> _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WBENTON <@t> umm.edu Thu Sep 3 12:26:03 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Thu Sep 3 12:26:38 2009 Subject: [Histonet] Re: Histonet Digest, Vol 70, Issue 4 INK ISSUE In-Reply-To: <040CF9A4.679@GWIA1.umm.edu> References: <040CF9A4.679@GWIA1.umm.edu> Message-ID: <4A9FC3EA.D886.00F4.3@umm.edu> Vinegar will work in place of Bouin's Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From carl.hobbs <@t> kcl.ac.uk Thu Sep 3 12:47:40 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Sep 3 12:48:12 2009 Subject: [Histonet] Re: pERK IHC Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A28@KCL-MAIL04.kclad.ds.kcl.ac.uk> Santa Cruz sc-101761. I tested it on FFPW sections of rat brain sections, after Citric acid HIER and it is very good ( if the results are specific, of course;-) It is stated as being Human/Mouse/rat reactive. I have two images posted here : http://www.immunoportal.com/index.php for this Ab. Sure, one has to join to view, I think. This is because the site kept getting heavy auto- Spamming. The owner has had to add hopefully more than adequate non-human filters; great respect to the person who set up this site and runs it so well! The link is safe, I hasten to add. carl NB: Until recently, I certainly did not appreciate how much continual hard work goes into constructing/maintaining any website such as the ones we Users acess so easily. Great respect/admiration for them that do this for us, so we can access information/data so much more easily. From Beth.Fye <@t> HCAhealthcare.com Thu Sep 3 12:51:04 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Thu Sep 3 12:51:07 2009 Subject: [Histonet] Dragon Message-ID: <938F8EC5A524D34EB5796E23E52781D329275EA8E6@NADCWPMSGCMS05.hca.corpad.net> Some of you have given very good responses to the Dragon question. May I ask an additional detail? Do any of you have Meditech as your information system, and if you do, did it have any particular problems when implementing? We have been trying to implement for a couple of months. There are several reasons I think we have been unsuccessful at this point, one being technical problems with Meditech. If anyone has any advice in particular for Meditech, I would be very appreciative!!! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From Janice.Mahoney <@t> alegent.org Thu Sep 3 12:52:25 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Sep 3 12:52:33 2009 Subject: [Histonet] RE: Dragon In-Reply-To: <938F8EC5A524D34EB5796E23E52781D329275EA8E6@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D329275EA8E6@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE59@EXCHMBC2.ad.ah.local> I have the same question for the Dolbe system. Jan Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, September 03, 2009 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dragon Some of you have given very good responses to the Dragon question. May I ask an additional detail? Do any of you have Meditech as your information system, and if you do, did it have any particular problems when implementing? We have been trying to implement for a couple of months. There are several reasons I think we have been unsuccessful at this point, one being technical problems with Meditech. If anyone has any advice in particular for Meditech, I would be very appreciative!!! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From shshaw <@t> WPI.EDU Thu Sep 3 13:09:45 2009 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Thu Sep 3 13:09:05 2009 Subject: [Histonet] Decal Solution Message-ID: Hi Histo world, I have a crazy question, I work in research facility and one of the investigators needs decal done on mice legs, they never check to see if we had decal solution before they began the procedure, of course we don't. I tried different companies to order some but they can't overnight the solution because it is a hazard. Does anyone have a protocol that they use in making a decal solution? The old histology books do but I was looking for maybe an updated solution. Please send my your suggestions they want this done ASAP. Thanks, Sharon Shaw WPI From Jackie.O'Connor <@t> abbott.com Thu Sep 3 13:13:15 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Sep 3 13:13:40 2009 Subject: [Histonet] Decal Solution In-Reply-To: Message-ID: I have decalled whole mouse legs (soft tissue removed). Use 5% formic acid for 24 hours on a shaker table. "Shaw, Sharon" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/03/2009 01:09 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Decal Solution Hi Histo world, I have a crazy question, I work in research facility and one of the investigators needs decal done on mice legs, they never check to see if we had decal solution before they began the procedure, of course we don't. I tried different companies to order some but they can't overnight the solution because it is a hazard. Does anyone have a protocol that they use in making a decal solution? The old histology books do but I was looking for maybe an updated solution. Please send my your suggestions they want this done ASAP. Thanks, Sharon Shaw WPI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Thu Sep 3 14:13:39 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Sep 3 14:14:44 2009 Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 References: <5D64396A0D4A5346BEBC759022AAEAA57C2E84@ITSSSXM01V6.one.ads.che.org> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8FC7B491@GIEXCHANGE.gidocs.net> I would completely agree, to make Dragon work you must want it to work. We use it successfully with AP Easy in the pathology lab, and successfully with Gmed in the rest of the office. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, September 03, 2009 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 For those who have a positive experience, would you please tell us what LIS you are using? We have partial success with it. We have GE Ultra (formerly Triple G). Some of our docs have issues with the program locking up and having to completely shut down the computer several times a day, so they gave up in frustration. They are the most verbose, of course. We have just tested a patch and will be installing it soon. Maybe that will help. Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Thursday, September 03, 2009 11:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 3 Judy, We have been using Dragon also for about the same length of time mentioned by Michael. All pathologists, cytotechs and PA's use it. It is a fantastic program if used properly and with the right attitude. Facts: 1) There are some words that it will always have problems with ie. Tan/ten/tin/ for example are difficult to distinguish between. 2) If in your mind it's not going to work - It won't! It knows :) 3) Creation of Macros, templates and commands make it a breeze. 4) Removing words from its dictionary. Words that you will never ever use can be very helpful when Dragon interprets words with something bizarre. 5) Or the opposite: train a command with a bizarre word ie. "BINGO" so that a command won't be performed in error by speaking a word that could be found in routine dictation. 6) Train it for phrases, rather than single words As you can see there are a lot of little "tricks" that we would be happy to pass on that will make the transition less frustrating. Use other's experiences to expedite your process and I think you too will find it's awesome. Message: 22 Date: Thu, 3 Sep 2009 06:51:20 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Dragon dictating system To: "'judy webb'" , "'histonet'" Message-ID: <001801ca2c84$790ab310$6b201930$@com> Content-Type: text/plain; charset="iso-8859-1" Judy, my company and I have been experimenting with Dragon since version 4. The current version is 10. The bottom line summary is Yes, it will work if you 1. put a lot of time in it, or 2. use a lot of macros/shortcuts/items of that nature. When it works, it's pretty awesome. If you need more details, please let me know. I'd be happy to share. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of judy webb Sent: Wednesday, September 02, 2009 9:27 PM To: histonet Subject: [Histonet] Dragon dictating system Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney John Peter Smith Hospital Fort Worth Texas 817-927-1024 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Sep 3 15:36:36 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Sep 3 15:36:59 2009 Subject: [Histonet] RE: Dragon In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE59@EXCHMBC2.ad.ah.local> References: <938F8EC5A524D34EB5796E23E52781D329275EA8E6@NADCWPMSGCMS05.hca.corpad.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE59@EXCHMBC2.ad.ah.local> Message-ID: <00ac01ca2cd6$3c9cc150$b5d643f0$@com> Here's kind of a technical answer for you guys. Dragon works with Microsoft Word so if any of your systems use WORD then Dragon should work fine. Additionally, Dragon works with VB based software, plus some others. Are you guys saying it only works 'sorta okay' or are you saying/wondering if it works at all? Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Thursday, September 03, 2009 1:52 PM To: 'Fye Beth'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dragon I have the same question for the Dolbe system. Jan Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, September 03, 2009 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dragon Some of you have given very good responses to the Dragon question. May I ask an additional detail? Do any of you have Meditech as your information system, and if you do, did it have any particular problems when implementing? We have been trying to implement for a couple of months. There are several reasons I think we have been unsuccessful at this point, one being technical problems with Meditech. If anyone has any advice in particular for Meditech, I would be very appreciative!!! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 3 15:56:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 3 15:56:44 2009 Subject: [Histonet] Your expert advice please_histology systems In-Reply-To: <582158.68197.qm@web95116.mail.in2.yahoo.com> Message-ID: <680425.69102.qm@web65712.mail.ac4.yahoo.com> Always Sakura. Ren? J. --- On Thu, 9/3/09, abi jag wrote: From: abi jag Subject: [Histonet] Your expert advice please_histology systems To: histonet@lists.utsouthwestern.edu Date: Thursday, September 3, 2009, 10:48 AM Dear Histonetters, We have planned to procure one set of histology systems for our laboratory(We have the existing one set of instruments all from Leica). We are considering the purchase of Tissue-Tek? VIP? 6(sakura) for tissue processing and Robot-Stainer HMS 740(from microm). Any of our members can share their experience with these instruments to me to enable us for finalizing. Thanks a lot for being in this team. Abijag abijag76@yahoo.co.in ? ? ? See the Web's breaking stories, chosen by people like you. Check out Yahoo! Buzz. http://in.buzz.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alfredpe <@t> mail.med.upenn.edu Thu Sep 3 17:03:58 2009 From: alfredpe <@t> mail.med.upenn.edu (Alfred Penzo Mendez) Date: Thu Sep 3 17:04:01 2009 Subject: [Histonet] Help with fixed-frozen tissue sections In-Reply-To: <1970585583.5455411252015019033.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Message-ID: <1126071037.5458491252015438185.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Dear Histonet members, for a few years I have done IHC on fixed-frozen mouse embryos with good success. A few days ago I moved onto adult tissues (liver and pancreas) and have met diffulties getting sections of good qualitiy. Here is how I proceeded: - Pancreas and liver were fixed whole, in Zinc-formalin (polysciences) for 2 hours at room temp. - Fixative was washed in PBS, 4 X 10 min. - cryoprotection: 15% sucrose PBS until in sinks (~1 hour), followed by 30% sucrose o/n then 1 hour in 1:1 sucrose/OCT. - Embedding: I blot excess sucrose/OCT with paper and then embedd in OCT. I like to place my mold on a thin plastic raft (the type that you use to wheigh powders)which I float on liquid nitrogen. This way the bloc freezes in 1 to 2 minutes. - Sectioning: I cut at 7 um. I used to cut embryos at -20 oC, but found that liver/pancvreas won't cut above -15 oC (they become hard and I get Venitian blind artifact). - H&E stain: I aird dry my sections 1 hour, then fix 10 minutes in Zinc-formalin, wash in PBS and stain regressively with Gill's II. I differentiate in 0.12 N (1%) HCl in 70% ethanol, blueing is done in 0.25% ammonia. - THE PROBLEM: is twol fold. first the architectur of the tissue is damaged, liver cells become "separate" so structure of parenchyma and lobules is lost. Something similar happens to the acinar pancreas, with loss of shape of acini. I suspect this is tissue compression from ice, which I don't understand siince I cryoprotect (I think) properly. Second is that my section tend to detach during the H&E, specially during diufferentiation step they detach. I never had this problem whith embryos, which were barely fixed (20 min. or less). I put a picture of liver under "alfredo-liver-artefact"; I'd greatly appreciate any input. Many thanks (and apologies for the lengthy post), Alfredo Penzo Abramson Family Cancer Research Institute Philadelphia, PA. From macveigh <@t> usc.edu Thu Sep 3 18:23:00 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Sep 3 18:23:04 2009 Subject: [Histonet] Re: DAB and Nuclear staining Message-ID: <00ba01ca2ced$7a9be9b0$5c237d80@DFS66DD1> Hi Melissa, We use Mayer's hematoxylin for 5 min and then rinse in tap water for at least 5 min (to blue the stain). If your DAB stain is weak, you can counterstain for even shorter time. It works great! The other option would be 1% Methil Green in 0.1M Sodium Acetate. My Methil powder is extremely old and probably this is the reason the stain is very weak, but it makes great contrast with the brown of the DAB. Good luck Michelle ----- Original Message ----- From: "Melissa Mazan" To: Sent: Thursday, September 03, 2009 6:18 AM Subject: [Histonet] DAB and nuclear staining > Hi all, > I have been having a problem with counterstaining for nuclei - I'm > looking at mouse lung tissues and staining macrophages with F4/80 (rat > anti-mouse). I use a negative serum control and a rat IgG isotype > control. I see very clear staining of macrophages - but when I > counterstain with hematoxylin or nuclear red, everything becomes muddy > and I can no longer discern the DAB staining. Can anyone offer advice? I > am using the Vector hematoxylin, and have tried as little as 5 seconds. > Melissa From tgenade <@t> gmail.com Fri Sep 4 07:05:01 2009 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Sep 4 07:05:07 2009 Subject: [Histonet] re: Endogenous biotin blocking Message-ID: From: Sally Price Subject: [Histonet] Endogenous biotin blocking > Its been my understanding that blocking is only necessary when one is certain > that background staining is caused by endogenous biotin, but maybe I'm > off-base here. I look forward to eveyone's input. I have been working on brain sections and without first blocking the endogenous biotin I get very poor definition staining in sections and no discernible staining in whole mounts. Any tissue that is metabolically active will have large concentrations of biotin as it is a co-enzyme in many of the enzymes involved in carboxyl-group transfer (such as in glycolysis , the CAC and fatty acid metabolism). The simplest way to see if you need to block is to do the experiment with and without blocking. More than likely, you will need to block. This is my opinion as a biochemist not a veteran histologist (which I am not). Hope this helps. -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From ree3 <@t> leicester.ac.uk Fri Sep 4 08:45:52 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 4 08:45:59 2009 Subject: [Histonet] paraform Message-ID: <7722595275A4DD4FA225B92CDBF174A18C84CE7EE3@EXC-MBX3.cfs.le.ac.uk> Is it OK to store freshly prepared 4% paraformaldehyde aliquots at -20C?? Thanks Richard Edwards Leicester University....U.K. From MSHERWOOD <@t> PARTNERS.ORG Fri Sep 4 09:06:45 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Sep 4 09:06:54 2009 Subject: [Histonet] paraform In-Reply-To: <7722595275A4DD4FA225B92CDBF174A18C84CE7EE3@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A18C84CE7EE3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23CAB@PHSXMB30.partners.org> I routinely make up aliquots of Karnovsky's fixative (2.5% glutaraldehyde-2% paraformaldehyde solution) and freeze them. I have had no issue with fixation. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, September 04, 2009 9:46 AM To: Histonet Subject: [Histonet] paraform Is it OK to store freshly prepared 4% paraformaldehyde aliquots at -20C?? Thanks Richard Edwards Leicester University....U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Rcartun <@t> harthosp.org Fri Sep 4 09:47:19 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 4 09:47:26 2009 Subject: [Histonet] Storage of liquid nitrogen Message-ID: <4AA0F036.7400.0077.1@harthosp.org> We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation). Has anyone else experienced this? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From JWeems <@t> sjha.org Fri Sep 4 09:48:45 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Sep 4 09:49:10 2009 Subject: [Histonet] Storage of liquid nitrogen In-Reply-To: <4AA0F036.7400.0077.1@harthosp.org> References: <4AA0F036.7400.0077.1@harthosp.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57C3074@ITSSSXM01V6.one.ads.che.org> Not yet!! What in the world? J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, September 04, 2009 10:47 To: Histonet Subject: [Histonet] Storage of liquid nitrogen We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation). Has anyone else experienced this? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mpence <@t> grhs.net Fri Sep 4 09:59:18 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Sep 4 09:59:23 2009 Subject: [Histonet] Storage of liquid nitrogen In-Reply-To: <4AA0F036.7400.0077.1@harthosp.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3C01@is-e2k3.grhs.net> Did they give a reason? Can they show you that requirement in writing for Liquid N.? Not many places have outside ventilation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, September 04, 2009 9:47 AM To: Histonet Subject: [Histonet] Storage of liquid nitrogen We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation). Has anyone else experienced this? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Sep 4 10:03:58 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Sep 4 10:04:03 2009 Subject: [Histonet] Storage of liquid nitrogen In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3C01@is-e2k3.grhs.net> References: <4AA0F036.7400.0077.1@harthosp.org> <661949901A768E4F9CC16D8AF8F2838C017A3C01@is-e2k3.grhs.net> Message-ID: <000701ca2d70$ee07dc00$ca179400$@net> Check new OSHA regs and local regs. I have had some strange requests from our inspectors over the last few years. They are probably looking at the fact LN in a closed area with a leak could be fatal without realizing how quickly it will dissipate. We had a driver years ago who lost a leg when an LN tank blew up on the truck as he unloaded it. That is the only time I ever heard of an issue and the company admitted the tank was at fault and should have been out of use at the time. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, September 04, 2009 10:59 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] Storage of liquid nitrogen Did they give a reason? Can they show you that requirement in writing for Liquid N.? Not many places have outside ventilation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, September 04, 2009 9:47 AM To: Histonet Subject: [Histonet] Storage of liquid nitrogen We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation). Has anyone else experienced this? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Sep 4 10:04:26 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Sep 4 10:04:30 2009 Subject: [Histonet] Storage of liquid nitrogen In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA57C3074@ITSSSXM01V6.one.ads.che.org> References: <4AA0F036.7400.0077.1@harthosp.org> <5D64396A0D4A5346BEBC759022AAEAA57C3074@ITSSSXM01V6.one.ads.che.org> Message-ID: <527312.44583.qm@web1101.biz.mail.sk1.yahoo.com> This is to prevent nitrogen suffocation in the event of a tank leak in a closed space. Just more regs to make life harder. Put in an exhaust fan. PKP ________________________________ From: "Weems, Joyce" To: Histonet Sent: Friday, September 4, 2009 9:48:45 AM Subject: RE: [Histonet] Storage of liquid nitrogen Not yet!! What in the world?? J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, September 04, 2009 10:47 To: Histonet Subject: [Histonet] Storage of liquid nitrogen We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation).? Has anyone else experienced this?? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT? 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Sep 4 10:29:29 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 4 10:29:34 2009 Subject: [Histonet] GMAblocks Message-ID: <7722595275A4DD4FA225B92CDBF174A18C84CE7EEC@EXC-MBX3.cfs.le.ac.uk> Assuming that GMA blocks are stored under optimum conditions,i.e. with desiccant and at -20C; how long can they be stored and still obtain meaningful immunohistochemical staining?. Many thanks for any info Richard Edwards Leicester University...U.K. From anonwums1 <@t> gmail.com Fri Sep 4 10:45:42 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Sep 4 10:45:48 2009 Subject: [Histonet] paraform In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23CAB@PHSXMB30.partners.org> References: <7722595275A4DD4FA225B92CDBF174A18C84CE7EE3@EXC-MBX3.cfs.le.ac.uk> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23CAB@PHSXMB30.partners.org> Message-ID: <858249120909040845q258fb70fo4e0118f9ba3e6bc8@mail.gmail.com> I freeze my 4% PFA. Haven't had any troubles attributable to that yet. Adam On Fri, Sep 4, 2009 at 9:06 AM, Sherwood, Margaret wrote: > I routinely make up aliquots of Karnovsky's fixative (2.5% > glutaraldehyde-2% > paraformaldehyde solution) and freeze them. I have had no issue with > fixation. > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, > R.E. > Sent: Friday, September 04, 2009 9:46 AM > To: Histonet > Subject: [Histonet] paraform > > > Is it OK to store freshly prepared 4% paraformaldehyde aliquots at > -20C?? > Thanks > Richard Edwards > Leicester University....U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it > is > addressed. If you believe this e-mail was sent to you in error and the > e-mail > contains patient information, please contact the Partners Compliance > HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From iraz.aydin <@t> epfl.ch Fri Sep 4 12:01:57 2009 From: iraz.aydin <@t> epfl.ch (iraz.aydin@epfl.ch) Date: Fri Sep 4 12:02:04 2009 Subject: [Histonet] paraform In-Reply-To: <7722595275A4DD4FA225B92CDBF174A18C84CE7EE3@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A18C84CE7EE3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <20090904190157.12903wtnlmucq0at@webmail.epfl.ch> We always do it that way, freeze 10ml & 50ml aliquots. We haven't encountered any problems. Iraz Toprak Aydin EPFL SV ISREC Lausanne, Switzerland Quoting "Edwards, R.E." : > > Is it OK to store freshly prepared 4% paraformaldehyde aliquots at -20C?? > Thanks > Richard Edwards > Leicester University....U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From laurie <@t> conxis.com Fri Sep 4 12:02:02 2009 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Fri Sep 4 12:02:11 2009 Subject: [Histonet] CD11b Message-ID: <0EE730FC711E4E6EA4D9A9BB26D4C8F2.MAI@accuwebhosting.biz> Happy Friday. Does anyone out there do CD11b Staining on human tissue? If so which antibody do you use? Thank you, Laurie From Rick.Garnhart <@t> memorialhealthsystem.com Fri Sep 4 12:07:12 2009 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Fri Sep 4 12:07:18 2009 Subject: [Histonet] Pathologist Consultation Send out In-Reply-To: Message-ID: If your Pathologists bill for the professional component privately, and your hospital bills for the technical component, who pay for expert consultations of slides sent out? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From JWeems <@t> sjha.org Fri Sep 4 12:09:45 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Sep 4 12:10:11 2009 Subject: [Histonet] Pathologist Consultation Send out In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57C30ED@ITSSSXM01V6.one.ads.che.org> Our hospital does. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com Sent: Friday, September 04, 2009 13:07 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologist Consultation Send out If your Pathologists bill for the professional component privately, and your hospital bills for the technical component, who pay for expert consultations of slides sent out? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From koellingr <@t> comcast.net Fri Sep 4 12:29:23 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Sep 4 12:29:26 2009 Subject: [Histonet] Storage of liquid nitrogen In-Reply-To: <527312.44583.qm@web1101.biz.mail.sk1.yahoo.com> Message-ID: <56421873.1043031252085363442.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> In a previous lab, we went through that. In order to keep the large nitrogen tank in a small room that had 2 access doors, there had to be an oxygen monitoring inside the access points. Oxygen level had to be at some minimal level otherwise an alarm would sound. Just in case, with doors closed, there was a leak and someone walked into a room whose air was lacking of enough oxygen. Close the door, get groggy, no one around and that is that. Was a pain in rear but considering the expansion of liquid N2 to gaseous state and how much volume it then occupies, I guess it makes sense. There have been such accidents that have occured. Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Paula Pierce" To: "Histonet" Sent: Friday, September 4, 2009 8:04:26 AM GMT -08:00 US/Canada Pacific Subject: Re: [Histonet] Storage of liquid nitrogen This is to prevent nitrogen suffocation in the event of a tank leak in a closed space. Just more regs to make life harder. Put in an exhaust fan. PKP ________________________________ From: "Weems, Joyce" To: Histonet Sent: Friday, September 4, 2009 9:48:45 AM Subject: RE: [Histonet] Storage of liquid nitrogen Not yet!! What in the world? J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, September 04, 2009 10:47 To: Histonet Subject: [Histonet] Storage of liquid nitrogen We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation). Has anyone else experienced this? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From redward <@t> ucb.br Fri Sep 4 12:36:24 2009 From: redward <@t> ucb.br (Robert Edward Pogue) Date: Fri Sep 4 12:37:38 2009 Subject: [Histonet] Storage of liquid nitrogen References: <56421873.1043031252085363442.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <0C9E32CEFAAF894CBE806BEE2206F75E0396E314@supra.ucb.br> Could be worse...once upon a time a fire safety officer asked me to sign a declaration that in the event of a fire i could get to an extinguisher easily. I pointed out that this was not the case as I was at the end of a hall with no extinguisher between me and the exit, so he offered the solution that in the event of a fire I could use the liquid nitrogen (which was in a large tank in the next room) to put out the fire. He was serious. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of koellingr@comcast.net Sent: Fri 04/09/2009 14:29 To: Paula Pierce Cc: Histonet Subject: Re: [Histonet] Storage of liquid nitrogen In a previous lab, we went through that. In order to keep the large nitrogen tank in a small room that had 2 access doors, there had to be an oxygen monitoring inside the access points. Oxygen level had to be at some minimal level otherwise an alarm would sound. Just in case, with doors closed, there was a leak and someone walked into a room whose air was lacking of enough oxygen. Close the door, get groggy, no one around and that is that. Was a pain in rear but considering the expansion of liquid N2 to gaseous state and how much volume it then occupies, I guess it makes sense. There have been such accidents that have occured. Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Paula Pierce" To: "Histonet" Sent: Friday, September 4, 2009 8:04:26 AM GMT -08:00 US/Canada Pacific Subject: Re: [Histonet] Storage of liquid nitrogen This is to prevent nitrogen suffocation in the event of a tank leak in a closed space. Just more regs to make life harder. Put in an exhaust fan. PKP ________________________________ From: "Weems, Joyce" To: Histonet Sent: Friday, September 4, 2009 9:48:45 AM Subject: RE: [Histonet] Storage of liquid nitrogen Not yet!! What in the world? J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, September 04, 2009 10:47 To: Histonet Subject: [Histonet] Storage of liquid nitrogen We have been told by our Fire and Safety Department that we can no longer store our large liquid nitrogen tank in a closed room (no windows or outside ventilation). Has anyone else experienced this? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jely <@t> mdanderson.org Fri Sep 4 13:25:04 2009 From: jely <@t> mdanderson.org (Ely,Jeanenne C) Date: Fri Sep 4 13:25:11 2009 Subject: [Histonet] Cox-2 Antibody Message-ID: Dear Histonet- We have used the monoclonal Cox-2 antibody from BD Biosciences (cat # 610204) with success in FFPE tissues. We have used it to stain rabbit tissue, but it works in human tissue as well. HIER with citrate buffer, and 1:200 dilution. Jeanenne Ely, BS, HT(ASCP), QIHC Chief Histology Lab MD Anderson Cancer Center Veterinary Medicine & Surgery Unit 63 1515 Holcombe Blvd, Houston, TX 77030 (713) 792-2793 From oyomagab <@t> gmail.com Fri Sep 4 14:54:51 2009 From: oyomagab <@t> gmail.com (X S) Date: Fri Sep 4 14:54:56 2009 Subject: [Histonet] Re: pERK IHC Message-ID: <9b0a33400909041254v34acd4dcl910147543fe1dbb5@mail.gmail.com> I find your images at http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=P-ERK Do you use same antibody to stain chick spinal cord, or is this another one? Santa Cruz sc-101761. I tested it on FFPW sections of rat brain sections, after Citric acid HIER and it is very good ( if the results are specific, of course;-) It is stated as being Human/Mouse/rat reactive. I have two images posted here : http://www.immunoportal.com/index.php for this Ab. Sure, one has to join to view, I think. This is because the site kept getting heavy auto- Spamming. The owner has had to add hopefully more than adequate non-human filters; great respect to the person who set up this site and runs it so well! The link is safe, I hasten to add. carl NB: Until recently, I certainly did not appreciate how much continual hard work goes into constructing/maintaining any website such as the ones we Users acess so easily. Great respect/admiration for them that do this for us, so we can access information/data so much more easily. From SDrew <@t> uwhealth.org Fri Sep 4 14:58:46 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Fri Sep 4 14:58:50 2009 Subject: [Histonet] HNF-1 Message-ID: <738A7878143FF74BB77436E255743C1A1F7C80@UWHC-MAIL03.uwhis.hosp.wisc.edu> One of our pathologists has inquiring whether there a reference lab that offers the antibody HNF-1B? Thanks for any thumbs-up! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From baderbo <@t> gmail.com Fri Sep 4 17:38:17 2009 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Fri Sep 4 17:38:23 2009 Subject: [Histonet] Microtome Leitz 1512, knife/blade holder Message-ID: <3c7e700909041538h3250706bqf5f19d70fafb0e1d@mail.gmail.com> Need help, looking for knife/blade holder for Leitz 1512 Microtome. Does anybody know where to buy this, thanks Bader e-mail address: baderbo@gmail.com From anonwums1 <@t> gmail.com Fri Sep 4 22:37:34 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Sep 4 22:37:41 2009 Subject: [Histonet] Staining with two primary antibodies from same host Message-ID: <858249120909042037x1490d814r5ff4cdbd56938614@mail.gmail.com> Hi all, I'm looking into staining with two primary antibodies from the same host, in this case goat. I've read a bit about this on Jackson Immunoresearch's website, but I wanted to run by my idea to get an idea if this is at all feasible. I want to stain mouse tissue with antigen X and antigen Y. I have a two polyclonal primaries: goat anti-mouse X and a goat anti-mouse Y. This is what I was thinking 1) Block in donkey serum for 1 hr at room temp. 2) Incubate with goat anti-mouse X overnight at 4C. Wash. 3) Incubate with Dylight 488 donkey anti-goat for 30 mins at room temp. Wash. 4) Reblock in donkey serum for 1 hr at room temp. 5) Incubate with 10 - 20 ug / mL unconjugated donkey anti-goat Fab for 1 hr at room temp -- cheapest I could find was at Rockland. Wash. 6) Incubate with goat anti-mouse Y overnight at 4C. Wash. 7) Incubate with biotin donkey anti-goat for 30 mins at room temp. Wash. 8) Incubate with avidin AMCA for 30 mins at room temp Would this work? Is there an easier or better way? What are the pitalls or tips you could offer? Hope you all aren't reading this during your long weekend, Adam From angelariggin <@t> ymail.com Sat Sep 5 09:49:48 2009 From: angelariggin <@t> ymail.com (Angela Riggin) Date: Sat Sep 5 09:49:53 2009 Subject: [Histonet] Please remove me from the list Message-ID: <206660.8457.qm@web59902.mail.ac4.yahoo.com> Sent from my iPhone From histonet.nospam <@t> vneubert.com Sat Sep 5 10:05:39 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Sat Sep 5 10:05:48 2009 Subject: [Histonet] Please remove me from the list In-Reply-To: <206660.8457.qm@web59902.mail.ac4.yahoo.com> References: <206660.8457.qm@web59902.mail.ac4.yahoo.com> Message-ID: <4AA27E43.6000100@vneubert.com> I'm sure this will work from your iPhone too: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Follow the instructions on the bottom of the page for unsubscribing :) Why leave Histonet, anyway? Have a nice weekend, Valentin Angela Riggin wrote: > Sent from my iPhone > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carl.hobbs <@t> kcl.ac.uk Sat Sep 5 12:26:29 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Sep 5 12:27:31 2009 Subject: [Histonet] Re: pERK IHC Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A33@KCL-MAIL04.kclad.ds.kcl.ac.uk> Re other IP chick images...I think that they were from using Millipore's 06-182. I was concerned that blood vessels were also positive... so I just dropped it. Any ideas? carl From Rcartun <@t> harthosp.org Sun Sep 6 09:58:33 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Sep 6 09:58:41 2009 Subject: [Histonet] Pathologist Consultation Send out In-Reply-To: References: Message-ID: <4AA395D8.7400.0077.1@harthosp.org> Most pathologists that bill separately have a contract with the hospital to provide services. Therefore, in my opinion, if the pathologist can't provide a diagnosis and the case is sent out, the pathologist is responsible for the bill. However, if the case is sent out at the request of the patient or clinician (after the pathologist has established a diagnosis) then the patient (or her/his insurance) or the clinician is responsible for the bill. The hospital should not have to pay for these requests. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 9/4/2009 1:07 PM >>> If your Pathologists bill for the professional component privately, and your hospital bills for the technical component, who pay for expert consultations of slides sent out? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Mon Sep 7 02:35:46 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Sep 7 02:36:13 2009 Subject: [Histonet] RE: Staining with two primary antibodies from same host Message-ID: Adam,Good point to prepare a protocol first, and then start staining! From theoretical point of view your protocol should work. However, I have tried this 'Jackson approach' using a Fab blocking step in a mouse-on-mouse situation (before the MOM kits were available) without success. There are at least two good solutions for double immunofluorescence using two primaries from the same species:Brouns et al. JHC 50:575-582, 2002 and Uchihara et al. JHC 51:1201-1206, 2003 applied a tyramide/fluorochrome detection for the first primary and a simple two-step for the second. Because the tyramide amplification is such a sensitive method, the first primary can be diluted up to a level that the second simple two-step detection cannot pick up signal from the first primary. Titration of the first primary antibody is most important in this procedure. You can in vitro label your goat primary with the Zenon (Invitrogen) kit based on anti-goat Fab fragments directly labeled with an Alexa fluorochrome. Next, you can built up a multistep indirect/direct double staining method:goat primary 1donkey anti-goat/fluorochrome 1normal goat serum (1:10) for blockinggoat primary 2-Zenon in vitro labeled with anti-goat Fab/fluorochrome 2lots of success with staining!ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 4 Sep 2009 22:37:34 -0500 From: "Adam ." Subject: [Histonet] Staining with two primary antibodies from same host To: histonet@lists.utsouthwestern.edu Hi all, I'm looking into staining with two primary antibodies from the same host, in this case goat. I've read a bit about this on Jackson Immunoresearch's website, but I wanted to run by my idea to get an idea if this is at all feasible. I want to stain mouse tissue with antigen X and antigen Y. I have a two polyclonal primaries: goat anti-mouse X and a goat anti-mouse Y. This is what I was thinking 1) Block in donkey serum for 1 hr at room temp. 2) Incubate with goat anti-mouse X overnight at 4C. Wash. 3) Incubate with Dylight 488 donkey anti-goat for 30 mins at room temp. Wash. 4) Reblock in donkey serum for 1 hr at room temp. 5) Incubate with 10 - 20 ug / mL unconjugated donkey anti-goat Fab for 1 hr at room temp -- cheapest I could find was at Rockland. Wash. 6) Incubate with goat anti-mouse Y overnight at 4C. Wash. 7) Incubate with biotin donkey anti-goat for 30 mins at room temp. Wash. 8) Incubate with avidin AMCA for 30 mins at room temp Would this work? Is there an easier or better way? What are the pitalls or tips you could offer? Hope you all aren't reading this during your long weekend, Adam From rsrichmond <@t> gmail.com Mon Sep 7 13:58:27 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Sep 7 13:58:31 2009 Subject: [Histonet] Re: Staining with two primary antibodies from same host Message-ID: Well, a country pathologist like myself will never seen such a complex staining routine, but I'd sure like to design a graphic for a lecture about it! I'd definitely start with the Bremen Town Musicians from Grimm - http://www.elene-tlc.net/Bremen_Forum/Free_time_in_Bremen/The_Brementown_Musicians Bob Richmond Samurai Pathologist Knoxville TN From anh2006 <@t> med.cornell.edu Mon Sep 7 14:09:24 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Sep 7 14:09:28 2009 Subject: [Histonet] RE: Staining with two primary antibodies from same host In-Reply-To: References: Message-ID: I routinely stain with two primaries from same species. Mostly rat antibodies on mouse tissue. I have been fortunate enough so far not to have to do it in GFP containing tissue so I have utilized either commercially available FITc labeled primaries, or we label them ourselves. Then I use an anti-FITC Alexa 488 for boosting signal. Very nice results, some of which we have published. Here is the general outline of the protocol (email me if you want specifics): - Protein block - Unconjugated primary #1 antibody (for example rat anti-X) - Fluorophore conjugated secondary antibody (for example anti-rat IgG conjugated to CY3) - Block with Rat IgG in excess to block any sites on the secondary which could potentially bind to your second primary antibody. - FITC conjugated primary #2 (for example rat anti-Y-FITC labeled) - Alexa 488 conjugated anti-FITC X protein will be labeled red Y protein will be labeled green > >From: "Adam ." >Subject: [Histonet] Staining with two primary antibodies from same >host >To: histonet@lists.utsouthwestern.edu > >Hi all, > >I'm looking into staining with two primary antibodies from the same host, in >this case goat. I've read a bit about this on Jackson Immunoresearch's >website, but I wanted to run by my idea to get an idea if this is at all >feasible. > >I want to stain mouse tissue with antigen X and antigen Y. I have a two >polyclonal primaries: goat anti-mouse X and a goat anti-mouse Y. This is >what I was thinking > >1) Block in donkey serum for 1 hr at room temp. >2) Incubate with goat anti-mouse X overnight at 4C. Wash. >3) Incubate with Dylight 488 donkey anti-goat for 30 mins at room temp. >Wash. >4) Reblock in donkey serum for 1 hr at room temp. >5) Incubate with 10 - 20 ug / mL unconjugated donkey anti-goat Fab for 1 hr >at room temp -- cheapest I could find was at >Rockland. >Wash. >6) Incubate with goat anti-mouse Y overnight at 4C. Wash. >7) Incubate with biotin donkey anti-goat for 30 mins at room temp. Wash. >8) Incubate with avidin AMCA for 30 mins at room temp > >Would this work? Is there an easier or better way? What are the pitalls or >tips you could offer? > >Hope you all aren't reading this during your long weekend, >Adam >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From tifei <@t> foxmail.com Mon Sep 7 20:40:13 2009 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Mon Sep 7 20:42:39 2009 Subject: [Histonet] RE: Staining with two primary antibodies from same host Message-ID: How to label the antibody with FITC? it is hard, or a complex procedure ------------------ Original ------------------ From: "Andrea Hooper"; Date: Tue, Sep 8, 2009 03:09 AM To: "Histonet"; Subject: [Histonet] RE: Staining with two primary antibodies from same host I routinely stain with two primaries from same species. Mostly rat antibodies on mouse tissue. I have been fortunate enough so far not to have to do it in GFP containing tissue so I have utilized either commercially available FITc labeled primaries, or we label them ourselves. Then I use an anti-FITC Alexa 488 for boosting signal. Very nice results, some of which we have published. Here is the general outline of the protocol (email me if you want specifics): - Protein block - Unconjugated primary #1 antibody (for example rat anti-X) - Fluorophore conjugated secondary antibody (for example anti-rat IgG conjugated to CY3) - Block with Rat IgG in excess to block any sites on the secondary which could potentially bind to your second primary antibody. - FITC conjugated primary #2 (for example rat anti-Y-FITC labeled) - Alexa 488 conjugated anti-FITC X protein will be labeled red Y protein will be labeled green > >From: "Adam ." >Subject: [Histonet] Staining with two primary antibodies from same >host >To: histonet@lists.utsouthwestern.edu > >Hi all, > >I'm looking into staining with two primary antibodies from the same host, in >this case goat. I've read a bit about this on Jackson Immunoresearch's >website, but I wanted to run by my idea to get an idea if this is at all >feasible. > >I want to stain mouse tissue with antigen X and antigen Y. I have a two >polyclonal primaries: goat anti-mouse X and a goat anti-mouse Y. This is >what I was thinking > >1) Block in donkey serum for 1 hr at room temp. >2) Incubate with goat anti-mouse X overnight at 4C. Wash. >3) Incubate with Dylight 488 donkey anti-goat for 30 mins at room temp. >Wash. >4) Reblock in donkey serum for 1 hr at room temp. >5) Incubate with 10 - 20 ug / mL unconjugated donkey anti-goat Fab for 1 hr >at room temp -- cheapest I could find was at >Rockland. >Wash. >6) Incubate with goat anti-mouse Y overnight at 4C. Wash. >7) Incubate with biotin donkey anti-goat for 30 mins at room temp. Wash. >8) Incubate with avidin AMCA for 30 mins at room temp > >Would this work? Is there an easier or better way? What are the pitalls or >tips you could offer? > >Hope you all aren't reading this during your long weekend, >Adam >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Sep 8 07:27:03 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Sep 8 07:27:50 2009 Subject: SPAM-LOW: [Histonet] RE: Staining with two primary antibodies from same host In-Reply-To: References: Message-ID: I would add an FC block before the first primary, I use one from Innovex for 20-30 min before the serum blocks, it is expensive but worth it for a mouse on mouse detection system. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Monday, September 07, 2009 1:36 AM To: anonwums1@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: Staining with two primary antibodies from same host Adam,Good point to prepare a protocol first, and then start staining! From theoretical point of view your protocol should work. However, I have tried this 'Jackson approach' using a Fab blocking step in a mouse-on-mouse situation (before the MOM kits were available) without success. There are at least two good solutions for double immunofluorescence using two primaries from the same species:Brouns et al. JHC 50:575-582, 2002 and Uchihara et al. JHC 51:1201-1206, 2003 applied a tyramide/fluorochrome detection for the first primary and a simple two-step for the second. Because the tyramide amplification is such a sensitive method, the first primary can be diluted up to a level that the second simple two-step detection cannot pick up signal from the first primary. Titration of the first primary antibody is most important in this procedure. You can in vitro label your goat primary with the Zenon (Invitrogen) kit based on anti-goat Fab fragments directly labeled with an Alexa fluorochrome. Next, you can built up a multistep indirect/direct double staining method:goat primary 1donkey anti-goat/fluorochrome 1normal goat serum (1:10) for blockinggoat primary 2-Zenon in vitro labeled with anti-goat Fab/fluorochrome 2lots of success with staining!ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 4 Sep 2009 22:37:34 -0500 From: "Adam ." Subject: [Histonet] Staining with two primary antibodies from same host To: histonet@lists.utsouthwestern.edu Hi all, I'm looking into staining with two primary antibodies from the same host, in this case goat. I've read a bit about this on Jackson Immunoresearch's website, but I wanted to run by my idea to get an idea if this is at all feasible. I want to stain mouse tissue with antigen X and antigen Y. I have a two polyclonal primaries: goat anti-mouse X and a goat anti-mouse Y. This is what I was thinking 1) Block in donkey serum for 1 hr at room temp. 2) Incubate with goat anti-mouse X overnight at 4C. Wash. 3) Incubate with Dylight 488 donkey anti-goat for 30 mins at room temp. Wash. 4) Reblock in donkey serum for 1 hr at room temp. 5) Incubate with 10 - 20 ug / mL unconjugated donkey anti-goat Fab for 1 hr at room temp -- cheapest I could find was at Rockland. Wash. 6) Incubate with goat anti-mouse Y overnight at 4C. Wash. 7) Incubate with biotin donkey anti-goat for 30 mins at room temp. Wash. 8) Incubate with avidin AMCA for 30 mins at room temp Would this work? Is there an easier or better way? What are the pitalls or tips you could offer? Hope you all aren't reading this during your long weekend, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JPeters <@t> bostwicklaboratories.com Tue Sep 8 07:31:16 2009 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Tue Sep 8 07:31:19 2009 Subject: [Histonet] CD33 antibody Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F06ABCE50@mail1.BOSTWICK.COM> I am having some trouble getting good staining with the CD33 antibody from Novocastra. I am trying to stain FFPE bone marrow biopsies decalcified in Formical for 1 hour prior to processing. The staining is very weak even at a 1:25 dilution using a pH 9.0 antigen retrieval solution (recommended) in a pressure cooker. Has anyone had any luck with this antibody on bone marrow? I have tried this protocol with the blood clots sent with the bone marrow biopsies and it works just fine so I am guessing that the decal is affecting the staining. Any thoughts?? Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com From trathborne <@t> somerset-healthcare.com Tue Sep 8 09:55:23 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 8 09:55:30 2009 Subject: [Histonet] Biocare Medical Antibodies Message-ID: Hi All, Is anyone using the Biocare cocktails with their Ventana stainers? Particularly the ULTRA? What success or problems have you encountered? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From leiker <@t> buffalo.edu Tue Sep 8 10:28:34 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Sep 8 10:28:40 2009 Subject: [Histonet] RE: Staining with two primary antibodies from same host In-Reply-To: References: Message-ID: <2E0F1000A321137B50364D9D@CDYwxp1931.ad.med.buffalo.edu> Nice! I may try one or both of your procedures (Adam & Andrea). ~Merced --On Monday, September 07, 2009 3:09 PM -0400 Andrea Hooper wrote: > I routinely stain with two primaries from same species. Mostly rat > antibodies on mouse tissue. I have been fortunate enough so far not to > have to do it in GFP containing tissue so I have utilized either > commercially available FITc labeled primaries, or we label them > ourselves. Then I use an anti-FITC Alexa 488 for boosting signal. Very > nice results, some of which we have published. > > Here is the general outline of the protocol (email me if you want > specifics): > > - Protein block > - Unconjugated primary #1 antibody (for example rat anti-X) > - Fluorophore conjugated secondary antibody (for example anti-rat IgG > conjugated to CY3) > - Block with Rat IgG in excess to block any sites on the secondary which > could potentially bind to your second primary antibody. > - FITC conjugated primary #2 (for example rat anti-Y-FITC labeled) > - Alexa 488 conjugated anti-FITC > > X protein will be labeled red > Y protein will be labeled green > > > > >> >> From: "Adam ." >> Subject: [Histonet] Staining with two primary antibodies from same >> host >> To: histonet@lists.utsouthwestern.edu >> >> Hi all, >> >> I'm looking into staining with two primary antibodies from the same >> host, in this case goat. I've read a bit about this on Jackson >> Immunoresearch's website, but I wanted to run by my idea to get an idea >> if this is at all feasible. >> >> I want to stain mouse tissue with antigen X and antigen Y. I have a two >> polyclonal primaries: goat anti-mouse X and a goat anti-mouse Y. This is >> what I was thinking >> >> 1) Block in donkey serum for 1 hr at room temp. >> 2) Incubate with goat anti-mouse X overnight at 4C. Wash. >> 3) Incubate with Dylight 488 donkey anti-goat for 30 mins at room temp. >> Wash. >> 4) Reblock in donkey serum for 1 hr at room temp. >> 5) Incubate with 10 - 20 ug / mL unconjugated donkey anti-goat Fab for 1 >> hr at room temp -- cheapest I could find was at >> Rockland> rified-anti-goat-igg-28-805-7102-805-7102.htm>. Wash. >> 6) Incubate with goat anti-mouse Y overnight at 4C. Wash. >> 7) Incubate with biotin donkey anti-goat for 30 mins at room temp. Wash. >> 8) Incubate with avidin AMCA for 30 mins at room temp >> >> Would this work? Is there an easier or better way? What are the pitalls >> or tips you could offer? >> >> Hope you all aren't reading this during your long weekend, >> Adam >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From DKnutson <@t> primecare.org Tue Sep 8 10:49:09 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Sep 8 10:49:45 2009 Subject: [Histonet] Dragon Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B6769@exchangent> We are currently researching the Dragon voice recognition system and I see that there is quite a bit of recent emails on the histonet concerning this subject. Is there anyone who is using Dragon that experienced reluctant Pathologists and how did you deal with that? I would be very interested in the mini steps you may have taken to go paperless and bring the Pathologists on board with this new idea. Thank you for sharing your experiences and suggestions with me. This histonet is such a great tool!!! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From robert_schoonhoven <@t> yahoo.com Tue Sep 8 10:53:23 2009 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Tue Sep 8 10:53:27 2009 Subject: [Histonet] GMAblocks In-Reply-To: <7722595275A4DD4FA225B92CDBF174A18C84CE7EEC@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A18C84CE7EEC@EXC-MBX3.cfs.le.ac.uk> Message-ID: <697700.75265.qm@web31105.mail.mud.yahoo.com> Richard, I am assuming that you have already worked-up your antibodies on GMA embedded tissue...... If you have then you should be OK for months depending entirely on the epitope in question. If you have not then you could be in for an unpleasant surprise as many antibodies are not able to penetrate the polymerized GMA which cannot be etched. Robert Schoonhoven, HT/HTL (ASCP) ________________________________ From: "Edwards, R.E." To: Histonet Sent: Friday, September 4, 2009 11:29:29 AM Subject: [Histonet] GMAblocks Assuming that GMA blocks are stored under optimum conditions,i.e. with desiccant and at -20C; how long can they be stored and still obtain meaningful immunohistochemical staining?. Many thanks for any info Richard Edwards Leicester University...U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Aubrey <@t> nsh.org Tue Sep 8 12:17:13 2009 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Tue Sep 8 12:19:45 2009 Subject: [Histonet] NSH One Day Forum in NJ Message-ID: NSH is excited to announce a partnership with the New Jersey Society for Histotechnology to present a one day session dedicated to topics of interest for the histologist working in the research field. One low price includes continental breakfast and lunch. Sessions presented include: * The Use of In Situ Hybridization in Modern Drug Discovery, Dr. Paul Shughrue, Merck Research Laboratories Histologic Preparation of Decalcified Rat Incisors for Evaluating Compound Effects on Blood Vessel Density by IHC, Karen Phillips and Michele French, Bristol-Myers Squibb * Going LEAN in Research: 5S Visual Work Place, Carol Barone, Nemours - A. I. Dupont Hospital for Children * Insect Histology: Historical Overview and Current Perspectives, Damien Laudier, Laudier Histology * Troubleshooting IHC: Panel of Experts Panel Members: Maria Geraci-Erck, Schering-Plough, Tara Kennedy, Biocare Medical and Linda Watson, Bristol-Myers Squibb * Muscle Talk, Carol Barone, Nemours - A. I. Dupont Hospital for Children * A Histological Analysis of the Antiangiogenic Activity in Tumor Xenograft Models, Anne Lewin, Bristol-Myers Squibb For complete details visit www.nsh.org From rsrichmond <@t> gmail.com Tue Sep 8 12:24:42 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Sep 8 12:24:45 2009 Subject: [Histonet] Re: Dragon speech recognition Message-ID: In my travels as a locum tenens pathologist, I have not seen Dragon or any other speech recognition system in use by pathologists, and can only recall one client who was even considering it. Computerized speech recognition could be disastrous for a small pathology practice, if management were to use its introduction as an excuse to fire the transcriptionist, who also answers the telephone and is the de facto practice administrator as well. Speech recognition systems depend on good microphones and on a quiet work area with minimal extraneous noise. Grossing stations are inherently noisy, and the working conditions (vibration, formaldehyde) quickly degrade microphones. Many pathologists' hospital offices are also very noisy. I'd like to get my hands on a speech recognition system, and I think I could learn it. My problem with it would be that I can type pathology reports about as fast as I can dictate them. Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Tue Sep 8 12:31:41 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Sep 8 12:31:45 2009 Subject: [Histonet] Re: Dragon speech recognition Message-ID: In my travels as a locum tenens pathologist, I have not seen Dragon or any other speech recognition system in use by pathologists, and can only recall one client who was even considering it. Computerized speech recognition could be disastrous for a small pathology practice, if management were to use its introduction as an excuse to fire the transcriptionist, who also answers the telephone and is the de facto practice administrator as well. Speech recognition systems depend on good microphones and on a quiet work area with minimal extraneous noise. Grossing stations are inherently noisy, and the working conditions (vibration, formaldehyde) quickly degrade microphones. Many pathologists' hospital offices are also very noisy. I'd like to get my hands on a speech recognition system, and I think I could learn it. My problem with it would be that I can type pathology reports about as fast as I can dictate them. Bob Richmond Samurai Pathologist Knoxville TN From MOG <@t> afrri.usuhs.mil Tue Sep 8 12:51:18 2009 From: MOG <@t> afrri.usuhs.mil (MOG, STEVEN R.) Date: Tue Sep 8 12:51:54 2009 Subject: [Histonet] Job Opportunity - GS-0601-11 - Bethesda, MD Message-ID: <99B2AAB36DBB3642B9467660CA392791050A6D45@radm.afrri.usuhs.mil> There is a full-time Federal civilian job opening at a DoD research facility (Armed Forces Radiobiology Research Institute) in Bethesda, Maryland. The position is for a Histotechnologist (Comparative Pathology) GS 0601-11 and was opened up for recruitment recently. The below link provides the full recruitment announcement out on the Department of the Navy (DON) CHART system. https://chart.donhr.navy.mil/jobsearch/jobdetailE.asp?vid=93997 Opening Number: NW9-0601-11-K3475832-I Opening Date: 9/5/09 Closing Date: 9/12/09 Steven R. Mog, DVM, Diplomate ACVP MAJ, VC, USA Chief, Comparative Pathology Division Veterinary Sciences Department Armed Forces Radiobiology Research Institute (AFRRI) Uniformed Services University of the Health Sciences (USUHS) Bethesda, MD 20889 301-295-1568 From lpaveli1 <@t> hurleymc.com Tue Sep 8 12:53:36 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Sep 8 12:53:53 2009 Subject: [Histonet] Re: Dragon speech recognition Message-ID: <4AA661E0020000EE0002C3AA@smtp-gw.hurleymc.com> Our pathologists' currently use dragon speech recognition. The newest version has worked out worlds better than the old system. Even our pathologist from India, with a fairly heavy accent, has happily used it. I must say that the system 5-6 years ago, did not meet their expectations and they all soon quit. But the latest one has worked out very well. Our pathologist's assistant however, chose not to use it this time, as the first time around was just a nightmare for him. And, as Dr. Richmond said below, maybe it was due to the noisy, smelly conditions. My non-typing husband uses a home-version to dictate letters to friends! He loves it! >>> Robert Richmond 09/08/09 1:31 PM >>> In my travels as a locum tenens pathologist, I have not seen Dragon or any other speech recognition system in use by pathologists, and can only recall one client who was even considering it. Computerized speech recognition could be disastrous for a small pathology practice, if management were to use its introduction as an excuse to fire the transcriptionist, who also answers the telephone and is the de facto practice administrator as well. Speech recognition systems depend on good microphones and on a quiet work area with minimal extraneous noise. Grossing stations are inherently noisy, and the working conditions (vibration, formaldehyde) quickly degrade microphones. Many pathologists' hospital offices are also very noisy. I'd like to get my hands on a speech recognition system, and I think I could learn it. My problem with it would be that I can type pathology reports about as fast as I can dictate them. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Tue Sep 8 13:04:46 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Sep 8 13:04:51 2009 Subject: [Histonet] Re: Dragon speech recognition In-Reply-To: <4AA661E0020000EE0002C3AA@smtp-gw.hurleymc.com> Message-ID: Our two pathologists have just started using the Dragon speech recognition - the medical version that our hospital had purchased for other physicians. One of our pathologists, in trying out the software, read from a pathology textbook. It never missed a word. We have been using it for approximately three weeks now. It has eliminated a full time position (she had just given her notice one month ago). The other parts of the transcriptionist position have been absorbed by three laboratory secretaries. So far, the pathologists believe that using the Dragon software has decreased our turn around time. Lynne Bell, HT (ASCP) Central Vermont Medical Center 130 Fisher Road Berlin, VT 05602 From dellav <@t> musc.edu Tue Sep 8 16:39:24 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Sep 8 16:39:29 2009 Subject: [Histonet] Pathologist Consultation Send out In-Reply-To: <4AA395D8.7400.0077.1@harthosp.org> References: <4AA395D8.7400.0077.1@harthosp.org> Message-ID: Dr. Cartun's remarks describe the process followed at our facility. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Sunday, September 06, 2009 10:59 AM To: histonet@lists.utsouthwestern.edu; Rick.Garnhart@memorialhealthsystem.com Subject: Re: [Histonet] Pathologist Consultation Send out Most pathologists that bill separately have a contract with the hospital to provide services. Therefore, in my opinion, if the pathologist can't provide a diagnosis and the case is sent out, the pathologist is responsible for the bill. However, if the case is sent out at the request of the patient or clinician (after the pathologist has established a diagnosis) then the patient (or her/his insurance) or the clinician is responsible for the bill. The hospital should not have to pay for these requests. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 9/4/2009 1:07 PM >>> If your Pathologists bill for the professional component privately, and your hospital bills for the technical component, who pay for expert consultations of slides sent out? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ppinchr <@t> yahoo.com Tue Sep 8 19:00:50 2009 From: ppinchr <@t> yahoo.com (RP) Date: Tue Sep 8 19:00:53 2009 Subject: [Histonet] 1p/19q probe Message-ID: <423953.12085.qm@web37106.mail.mud.yahoo.com> I've been working on FISH on Her-2 neu and the neuro pathologist presumed I knew a protocol on Oligodendrogliomas. I was wondering if any one out there has used 1p 1q probe and if they have a protocol that they use on the Vysis 2000.? I would really appreciate it. Rachel Pinch HT From brian <@t> prometheushealthcare.com Tue Sep 8 19:56:08 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Tue Sep 8 19:56:12 2009 Subject: [Histonet] New Histology Opening in Brooklyn, NY Message-ID: <002301ca30e8$51ab7ee0$f5027ca0$@com> New Histology position available in Brooklyn, New York. This is a full time permanent position with a privately held lab. It is a day shift and has the possibility of transitioning into supervisor role. Please call or email if interested Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From sprice2003 <@t> gmail.com Wed Sep 9 06:14:15 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Sep 9 06:14:21 2009 Subject: [Histonet] Ventana Her2/neu Message-ID: On behalf of a colleaugue who doesn't participate in the Histonet, I'd like to ask folks if there experiencing difficulty validating their Pathyway Her2/neu antibody. My friend has tried two different lots and both are showing unusually weak staining. Just wondering if anyone else is having this issue and if you can offer some suggestions. From vonavi <@t> inbox.lv Wed Sep 9 06:20:24 2009 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Wed Sep 9 06:20:31 2009 Subject: [Histonet] Blood films, confocal microscopy Message-ID: <1252495224.4aa78f7811516@mail.inbox.lv> Hello, I would like to stain human blood cells (thin blood film) for confocal microscopy. Could anyone please share his/her own experience in this matter? I would highly appreciate this. Thank you in advance. Regards, Andrey From nyilmaz <@t> mersin.edu.tr Wed Sep 9 08:58:27 2009 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Wed Sep 9 08:58:43 2009 Subject: [Histonet] mercury vs led lights Message-ID: <000701ca3155$9c550840$e201640a@nejat1> Dear Colleagues, We're attempted to buy a new inverted fluorescence microscope for our research lab. Distrubitor of leica offered us a new type system working with led lights which is about same price. Does anybody know advantages and disadvantages of this system compared to conventional (with mercury lamp) fluorescence microscopes. Thanks in advance. Necat Yilmaz From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 9 10:43:53 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Sep 9 10:44:25 2009 Subject: [Histonet] mercury vs led lights In-Reply-To: <000701ca3155$9c550840$e201640a@nejat1> References: <000701ca3155$9c550840$e201640a@nejat1> Message-ID: <1AAF670737F193429070841C6B2ADD4CE169FF2E@EXMBMCB15.ucsfmedicalcenter.org> Necat, The advantages of LED's are: 1) The wavelength is pure in the spectrum the LED is designed for while mercury are quite variable across their spectrum. 2) LED's last many thousands of hours - in the range of 20,000 to 50,000 hours for most uses. This alone will lead to major expense reduction since mercury lamps, at $200 each, last a few hundred hours at best. Even 400 to 1200 hours for xenon lamps will be very expensive compared to LED's). In fact, depending on the level of microscope you get you may be able to pay for a microscope over it's lifetime just in lamp replacement savings. 3)LED's do not require any warmup or cooldown time and can switched on and off at will 4) You can use a dimmer control on LED's without affecting the spectrum. 4) Because of #2 and #3 LED's are very robust, easy to use and do not require any special care, unlike mercury and xenon lamps that require special training and handling to use correctly. LED's are perfect for student microscopes. In the past the major disadvantage of LED's was that they were not very intense/powerful (1W or so, equivalent to a 50W mercury burner) and could not be used in epi-fluroescence without losing most of their intensity as the light passes through all the glass lenses. Now much more powerful LED's are available (up to 5W) that are suitable for epi. Disadvantages: 1) You do need a specific LED lamp for each wavelength range you want to use. One LED will not cover the whole spectrum. 2) The microscope must be designed to use an LED lamp. However, there are adaptors available for some microscope models (see http://www.fraensrl.com/flmicro.html ), but only for bright field transmission mode. References: Light sources http://micro.magnet.fsu.edu/primer/anatomy/lightsourceshome.html Also http://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorosources.html LED's http://zeiss-campus.magnet.fsu.edu/articles/lightsources/leds.html LED's Tim Morken UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, September 09, 2009 6:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mercury vs led lights Dear Colleagues, We're attempted to buy a new inverted fluorescence microscope for our research lab. Distrubitor of leica offered us a new type system working with led lights which is about same price. Does anybody know advantages and disadvantages of this system compared to conventional (with mercury lamp) fluorescence microscopes. Thanks in advance. Necat Yilmaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Sep 9 11:09:25 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Sep 9 11:09:30 2009 Subject: [Histonet] Ventana Her2/neu In-Reply-To: References: Message-ID: <5b6eb13e0909090909k163725dcpd0f7a25eea539794@mail.gmail.com> We had the same problem with two dispensers of the same lot. I'd bet that his two dispensers are of the same lot. Ventana investigated and found that it was their lot of antibody that was bad. Last week they issued a recall of that lot. I'd suggest calling Ventana and asking if those dispensers are from that lot. The recall letter should have been received... he could look for that too... Mark Tarango On Wed, Sep 9, 2009 at 4:14 AM, Sally Price wrote: > On behalf of a colleaugue who doesn't participate in the Histonet, I'd like > to ask folks if there experiencing difficulty validating their Pathyway > Her2/neu antibody. My friend has tried two different lots and both are > showing unusually weak staining. Just wondering if anyone else is having > this issue and if you can offer some suggestions. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Wed Sep 9 11:21:09 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 9 11:21:14 2009 Subject: [Histonet] mercury vs led lights In-Reply-To: <000701ca3155$9c550840$e201640a@nejat1> References: <000701ca3155$9c550840$e201640a@nejat1> Message-ID: LEDs don't quench your fluorescence as quickly as mercury. --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz wrote: > Dear Colleagues, > > We're attempted to buy a new inverted fluorescence microscope for our > research lab. Distrubitor of leica offered us a new type system working > with led lights which is about same price. Does anybody know advantages > and disadvantages of this system compared to conventional (with mercury > lamp) fluorescence microscopes. > Thanks in advance. > > Necat Yilmaz > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From leiker <@t> buffalo.edu Wed Sep 9 12:43:45 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 9 12:43:51 2009 Subject: [Histonet] mercury vs led lights In-Reply-To: References: <000701ca3155$9c550840$e201640a@nejat1> Message-ID: <6276F2A000238523BC2681F5@CDYwxp1931.ad.med.buffalo.edu> I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted microscope we have LEDs and we also have a mercury lamp to cover the red range. The Zeiss switches nicely and very smoothly between the light sources during viewing and image acquisition. Sorry for the highly untechnical description, but that is all I know about it. :-) Regards, Merced --On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker wrote: > LEDs don't quench your fluorescence as quickly as mercury. > > > --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz > wrote: > >> Dear Colleagues, >> >> We're attempted to buy a new inverted fluorescence microscope for our >> research lab. Distrubitor of leica offered us a new type system working >> with led lights which is about same price. Does anybody know advantages >> and disadvantages of this system compared to conventional (with mercury >> lamp) fluorescence microscopes. >> Thanks in advance. >> >> Necat Yilmaz >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From araniqkslvr <@t> yahoo.com Wed Sep 9 13:10:05 2009 From: araniqkslvr <@t> yahoo.com (Paula) Date: Wed Sep 9 13:10:08 2009 Subject: [Histonet] HT Refresher Courses Anywhere Message-ID: <81387.66820.qm@web30404.mail.mud.yahoo.com> Hello, ? I am a registered HT but have not worked in the field a long time--late 80s. I have been?trying to get back into a lob?since 2007 when I lost my last job (typesetting, not lab work.) I have had a recruiter laugh at me. I have tired applying for histology assistant jobs. I had one recruiter tell me that I could be trained in the job IF I had a B.S. in science, but since I didn't, I couldn't be trained. Go figure. ? I have an A.A. in histotechnology from Harford Community College in Bel Air, MD, and a B.S. in liberal arts (english). I already checked with my A.A. school and they offer no refresher. ? I did a couple weeks in a lab last year but the travel was too far--Worcester, MA. I did use all the automated equipment that I never used before. ? Has anyone else tried to re-enter the field and had the same problems? I'm willing to pay a little for a refresher, but don't want to do the whole degree over again. ? BTW, I am in Milford, MA (metrowest, central)--a little too far from the Boston hospitals to work there (takes a train, AND subway and is just too long a commute). I don't like to commute very far.?My SO and I are also considering a move to North Carolina or Colorado, but we're not sure yet. ? Thanks, ? Paula From mlbukhar <@t> ucalgary.ca Wed Sep 9 13:20:55 2009 From: mlbukhar <@t> ucalgary.ca (maureen bukhari) Date: Wed Sep 9 13:21:03 2009 Subject: [Histonet] diff-quik stain Message-ID: <00c401ca317a$45a2d1b0$d0e87510$@ca> Does anyone out there in Histonet -land have a recipe to make my own diff-quik stain or a place to buy it. Is it marketed under another name? Thanks ahead, Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca From derek.papalegis <@t> tufts.edu Wed Sep 9 13:22:32 2009 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Wed Sep 9 13:22:36 2009 Subject: [Histonet] equipment pricing Message-ID: <4AA7F268.3050400@tufts.edu> I need some info for a possible future expansion and need some general prices on some equipment that we might purchase. Can anyone give me the list price of these pieces of equipment: Dako Autostainer Ventana NexEs IHC full system Sakura Tissue-Tek VIP 5 Sakura Tissue-Tek Glass coverslipper It is tough to get a definite answer from vendors concerning pricing if you don't know whether you will be purchasing soon or not. Thanks for the help. Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 9 13:25:08 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Sep 9 13:25:17 2009 Subject: [Histonet] mercury vs led lights In-Reply-To: <6276F2A000238523BC2681F5@CDYwxp1931.ad.med.buffalo.edu> References: <000701ca3155$9c550840$e201640a@nejat1> <6276F2A000238523BC2681F5@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <1AAF670737F193429070841C6B2ADD4CE16A0094@EXMBMCB15.ucsfmedicalcenter.org> Merced wrote: " I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor 555, Cy3, TRITC, etc.) This is per our Zeiss rep." I'm not sure why the Zeiss rep said that. Maybe Zeiss itself does not carry the LED's in that range but I have used up to 655nm LED. See this for details on other LED's for fluorescence work: http://www.fraensrl.com/images/fraen_fluorochromes.pdf Tim Morken UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, September 09, 2009 10:44 AM To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mercury vs led lights I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted microscope we have LEDs and we also have a mercury lamp to cover the red range. The Zeiss switches nicely and very smoothly between the light sources during viewing and image acquisition. Sorry for the highly untechnical description, but that is all I know about it. :-) Regards, Merced --On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker wrote: > LEDs don't quench your fluorescence as quickly as mercury. > > > --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz > wrote: > >> Dear Colleagues, >> >> We're attempted to buy a new inverted fluorescence microscope for our >> research lab. Distrubitor of leica offered us a new type system working >> with led lights which is about same price. Does anybody know advantages >> and disadvantages of this system compared to conventional (with mercury >> lamp) fluorescence microscopes. >> Thanks in advance. >> >> Necat Yilmaz >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Wed Sep 9 13:24:56 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Sep 9 13:36:09 2009 Subject: [Histonet] HT Refresher Courses Anywhere In-Reply-To: <81387.66820.qm@web30404.mail.mud.yahoo.com> References: <81387.66820.qm@web30404.mail.mud.yahoo.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1E74@exchange.cmc-nh.org> I would try contacting pathology supervisors directly and explain your situation. There may be some that would allow you to work as a volunteer or as a histology aide to upgrade your skills and experience. If I understood your credentials correctly, you are certified HT by ASCP. A good deal of Histotechnology, after the formal academics, is on the job training. A smart employer would recognize that it would be far less expensive, for them as an organization, to allow you to train and upgrade your skills at their facility than it would be to pay a recruiter to bring in a histotechnologist. Especially if you agreed to a 2 year employment agreement. You should market yourself this way. Your skill is valuable, it just needs a little dusting off and practice not a formal refresher training. This may require you to temporarily relocate for a few months rather than trying to commute. If you are serious about getting back in the histology workforce, this is a small price to pay. You might want to start with the larger academic hospitals in New England and work down from there. Hope this helps. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sent: Wednesday, September 09, 2009 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Refresher Courses Anywhere Hello, ? I am a registered HT but have not worked in the field a long time--late 80s. I have been?trying to get back into a lob?since 2007 when I lost my last job (typesetting, not lab work.) I have had a recruiter laugh at me. I have tired applying for histology assistant jobs. I had one recruiter tell me that I could be trained in the job IF I had a B.S. in science, but since I didn't, I couldn't be trained. Go figure. ? I have an A.A. in histotechnology from Harford Community College in Bel Air, MD, and a B.S. in liberal arts (english). I already checked with my A.A. school and they offer no refresher. ? I did a couple weeks in a lab last year but the travel was too far--Worcester, MA. I did use all the automated equipment that I never used before. ? Has anyone else tried to re-enter the field and had the same problems? I'm willing to pay a little for a refresher, but don't want to do the whole degree over again. ? BTW, I am in Milford, MA (metrowest, central)--a little too far from the Boston hospitals to work there (takes a train, AND subway and is just too long a commute). I don't like to commute very far.?My SO and I are also considering a move to North Carolina or Colorado, but we're not sure yet. ? Thanks, ? Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Sep 9 13:37:08 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Sep 9 13:37:18 2009 Subject: [Histonet] diff-quik stain In-Reply-To: <00c401ca317a$45a2d1b0$d0e87510$@ca> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> Message-ID: <000d01ca317c$89db3230$9d919690$@net> Statlab has a god one called Hema Qik. I have used it and it was very good and easy to use. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Wednesday, September 09, 2009 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diff-quik stain Does anyone out there in Histonet -land have a recipe to make my own diff-quik stain or a place to buy it. Is it marketed under another name? Thanks ahead, Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Wed Sep 9 13:41:24 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Sep 9 13:41:27 2009 Subject: [Histonet] Block ship out container Message-ID: <434803.39610.qm@web58607.mail.re3.yahoo.com> I am inquiring for another lab that is searching?for what looks like a block holder/container that?will hold?four blocks for shipping. Has anyone ever seen these or know where they can be found? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? From leiker <@t> buffalo.edu Wed Sep 9 13:45:24 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 9 13:45:31 2009 Subject: [Histonet] mercury vs led lights In-Reply-To: <1AAF670737F193429070841C6B2ADD4CE16A0094@EXMBMCB15.ucsfmedicalcenter.org> References: <000701ca3155$9c550840$e201640a@nejat1> <6276F2A000238523BC2681F5@CDYwxp1931.ad.med.buffalo.edu> <1AAF670737F193429070841C6B2ADD4CE16A0094@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <871EEB8F317FFD4FF883BF27@CDYwxp1931.ad.med.buffalo.edu> Interesting, Tim! (I could be wrong, but I was pretty sure the said that when I questioned him about it!) I just checked the Zeiss website and apparently they carry 10 diodes to emit light at a particular frequency, including the reds. Now I need to ask him again why we don't have the red LED! Links below: --On Wednesday, September 09, 2009 11:25 AM -0700 "Morken, Tim" wrote: > Merced wrote: " I forgot to mention. An LED can be made to excite FITC, > DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the > red (Alexa Fluor 555, Cy3, TRITC, etc.) This is per our Zeiss rep." > > > > I'm not sure why the Zeiss rep said that. Maybe Zeiss itself does not > carry the LED's in that range but I have used up to 655nm LED. > > See this for details on other LED's for fluorescence work: > > http://www.fraensrl.com/images/fraen_fluorochromes.pdf > > > > Tim Morken > UCSF Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M > Leiker Sent: Wednesday, September 09, 2009 10:44 AM > To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] mercury vs led lights > > I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 > (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor > 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted > microscope we have LEDs and we also have a mercury lamp to cover the red > range. The Zeiss switches nicely and very smoothly between the light > sources during viewing and image acquisition. > > Sorry for the highly untechnical description, but that is all I know > about it. :-) > > Regards, > Merced > --On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker > wrote: > >> LEDs don't quench your fluorescence as quickly as mercury. >> >> >> --On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz >> wrote: >> >>> Dear Colleagues, >>> >>> We're attempted to buy a new inverted fluorescence microscope for our >>> research lab. Distrubitor of leica offered us a new type system working >>> with led lights which is about same price. Does anybody know advantages >>> and disadvantages of this system compared to conventional (with mercury >>> lamp) fluorescence microscopes. >>> Thanks in advance. >>> >>> Necat Yilmaz >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician II >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From jclark <@t> pcnm.com Wed Sep 9 13:49:30 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Sep 9 13:49:42 2009 Subject: [Histonet] QIHC textbooks Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C010D370F@mail.pcnm.com> Is anyone in histoland willing to sell, or know where I can find a copy of Antigen Retrieval Techniques: Immunohistochemistry & Molecular Morphology by Shi, S., GU, J., and Taylor, C.R (2000). This is one of the recommended texts for the QIHC exam and it is no longer in print. I have been unable to get a copy through Amazon. Any ideas or suggestions would be greatly appreciated. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM From sfeher <@t> CMC-NH.ORG Wed Sep 9 13:50:05 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Sep 9 13:52:46 2009 Subject: [Histonet] diff-quik stain In-Reply-To: <00c401ca317a$45a2d1b0$d0e87510$@ca> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> Thermo Fisher has one called "3 Step Stain" good stain and if you want to use Methanol as a fixative, rather than the one that would be ordered as a kit, you can save money by only ordering the 3 Step Stain Solution A and 3 Step Stain Solution B. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Wednesday, September 09, 2009 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diff-quik stain Does anyone out there in Histonet -land have a recipe to make my own diff-quik stain or a place to buy it. Is it marketed under another name? Thanks ahead, Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Sep 9 14:08:48 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Sep 9 14:09:33 2009 Subject: [Histonet] diff-quik stain In-Reply-To: <00c401ca317a$45a2d1b0$d0e87510$@ca> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> Message-ID: <85442D64C6C9407D88276C3BDD73A89A@BryanPC> Diff-Quik is a minor modification of Field's stain. Go here for a recipe: http://www.thelabrat.com/protocols/FieldsStain.shtml There are two methods of using this stain which was used for malaria parasites fin thin and thick films. Both are given here, among other methods. http://www.hpa-standardmethods.org.uk/documents/bsopTP/pdf/bsoptp39.pdf I got this information from a simple Google search. Bryan Llewellyn ----- Original Message ----- From: "maureen bukhari" To: Sent: Wednesday, September 09, 2009 11:20 AM Subject: [Histonet] diff-quik stain > Does anyone out there in Histonet -land have a recipe to make my own > diff-quik stain or a place to buy it. Is it marketed under another name? > > Thanks ahead, > > > > Maureen Bukhari > > Phone: 403-210-6524 > > e-mail: mlbukhar@ucalgary.ca > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Sep 9 14:15:46 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Sep 9 14:14:05 2009 Subject: [Histonet] QIHC textbooks In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C010D370F@mail.pcnm.com> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C010D370F@mail.pcnm.com> Message-ID: <4AA7FEE2.2020500@umdnj.edu> Go to the public library and have them get for you on interlibrary loan. Really! The universities in NM probably have a copy, and since they are supported by your tax dollars, you can get it and read/copy the necessary pages. That is the way it works here in NJ. Might take a few weeks. OR try bookstores that specialize in out of print books. Alibris comes to mind or just google the title of the book. Geoff Joanne Clark wrote: > Is anyone in histoland willing to sell, or know where I can find a copy > of Antigen Retrieval Techniques: Immunohistochemistry & Molecular > Morphology by Shi, S., GU, J., and Taylor, C.R (2000). This is one of > the recommended texts for the QIHC exam and it is no longer in print. I > have been unable to get a copy through Amazon. Any ideas or suggestions > would be greatly appreciated. > > > > Joanne Clark, HT > > Histology Supervisor > > Pathology Consultants of New Mexico > > Roswell, NM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mike <@t> pathview.com Wed Sep 9 14:18:47 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Sep 9 14:19:36 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> Message-ID: <016401ca3182$6c086740$441935c0$@com> I need some help from those of you who work with Ventana stainers. The Ventana equipment requires the use of Ventana slide labels. You can't just stick your LIS slide label on the slide and expect the Ventana equipment to read the slide barcode and perform the required stain (yes, I am assuming an interface is in place). On the other hand, have any of you either: 1. placed the LIS slide label on the back side of the slide, or 2. Overlapped the LIS slide label with the Ventana slide label such that you can still read both the LIS and the Ventana barcodes. I have heard conflicting answers from multiple Ventana representatives as to whether one or both of the aforementioned mechanisms work. I appreciate your time. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? From marktarango <@t> gmail.com Wed Sep 9 14:24:57 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Sep 9 14:25:04 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <016401ca3182$6c086740$441935c0$@com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> Message-ID: <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> We currently put the LIS label on the back of the slide. We very recently got Ventana to help us create a new label template that has the Ventana barcode and our 2D LIS label all printed on Ventana's Ebar printer. We will start using the new labels in a few weeks. Ventana doesn't support the new labels but they did help our IT people figure out how to do it. Mark Tarango On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik wrote: > I need some help from those of you who work with Ventana stainers. > > The Ventana equipment requires the use of Ventana slide labels. You can't > just stick your LIS slide label on the slide and expect the Ventana > equipment to read the slide barcode and perform the required stain (yes, I > am assuming an interface is in place). On the other hand, have any of you > either: > > 1. placed the LIS slide label on the back side of the slide, or > 2. Overlapped the LIS slide label with the Ventana slide label such that > you can still read both the LIS and the Ventana barcodes. > > I have heard conflicting answers from multiple Ventana representatives as > to > whether one or both of the aforementioned mechanisms work. > > I appreciate your time. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From stephanie.d.rivera <@t> gsk.com Wed Sep 9 14:33:44 2009 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Wed Sep 9 14:34:12 2009 Subject: [Histonet] QIHC textbooks In-Reply-To: <4AA7FEE2.2020500@umdnj.edu> Message-ID: Dear Joanne, I agree with Geoff on the library. Also someone from histonet suggested using the Labvision.com Tutorial. It prepared me for the QIHC as well as study book series #5 from NSH (Immunohistochemistry, Enzyme Histochemistry). Hope this helps. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Geoff McAuliffe" Sent by: histonet-bounces@lists.utsouthwestern.edu 09-Sep-2009 15:15 To "Joanne Clark" cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] QIHC textbooks Go to the public library and have them get for you on interlibrary loan. Really! The universities in NM probably have a copy, and since they are supported by your tax dollars, you can get it and read/copy the necessary pages. That is the way it works here in NJ. Might take a few weeks. OR try bookstores that specialize in out of print books. Alibris comes to mind or just google the title of the book. Geoff Joanne Clark wrote: > Is anyone in histoland willing to sell, or know where I can find a copy > of Antigen Retrieval Techniques: Immunohistochemistry & Molecular > Morphology by Shi, S., GU, J., and Taylor, C.R (2000). This is one of > the recommended texts for the QIHC exam and it is no longer in print. I > have been unable to get a copy through Amazon. Any ideas or suggestions > would be greatly appreciated. > > > > Joanne Clark, HT > > Histology Supervisor > > Pathology Consultants of New Mexico > > Roswell, NM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Sep 9 15:11:44 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Sep 9 15:12:45 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> Message-ID: <018b01ca3189$d64a8910$82df9b30$@com> Ventana's ebar printer - tell me a little about that, please.. I don't think I'm going to like what I hear, but let me shut up and listen for a little bit. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 3:25 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels We currently put the LIS label on the back of the slide. We very recently got Ventana to help us create a new label template that has the Ventana barcode and our 2D LIS label all printed on Ventana's Ebar printer. We will start using the new labels in a few weeks. Ventana doesn't support the new labels but they did help our IT people figure out how to do it. Mark Tarango On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik wrote: I need some help from those of you who work with Ventana stainers. The Ventana equipment requires the use of Ventana slide labels. You can't just stick your LIS slide label on the slide and expect the Ventana equipment to read the slide barcode and perform the required stain (yes, I am assuming an interface is in place). On the other hand, have any of you either: 1. placed the LIS slide label on the back side of the slide, or 2. Overlapped the LIS slide label with the Ventana slide label such that you can still read both the LIS and the Ventana barcodes. I have heard conflicting answers from multiple Ventana representatives as to whether one or both of the aforementioned mechanisms work. I appreciate your time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vonavi <@t> inbox.lv Wed Sep 9 15:32:45 2009 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Wed Sep 9 15:32:51 2009 Subject: [Histonet] Blood smear, confocal microscopy Message-ID: <1252528365.4aa810eda4aed@mail.inbox.lv> Hello, I would like to stain human blood cells (thin blood film) for confocal microscopy. Could anyone please share his/her own experience in this matter? I would highly appreciate this. Thank you in advance. Regards, Andrey From contact <@t> excaliburpathology.com Wed Sep 9 15:43:36 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Sep 9 15:43:41 2009 Subject: [Histonet] Block ship out container In-Reply-To: <434803.39610.qm@web58607.mail.re3.yahoo.com> References: <434803.39610.qm@web58607.mail.re3.yahoo.com> Message-ID: <135029.50877.qm@web1102.biz.mail.sk1.yahoo.com> Cocoon boxes may work. www.cocoonbox.net Look at the envelope boxes. They come in different sizes too. PKP ________________________________ From: Kelly Boyd To: histonet@lists.utsouthwestern.edu Sent: Wednesday, September 9, 2009 1:41:24 PM Subject: [Histonet] Block ship out container I am inquiring for another lab that is searching?for what looks like a block holder/container that?will hold?four blocks for shipping. Has anyone ever seen these or know where they can be found? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Wed Sep 9 16:18:38 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Sep 9 16:18:42 2009 Subject: [Histonet] diff-quik stain In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> Message-ID: <905508.75616.qm@web33807.mail.mud.yahoo.com> Mercedes Medical has a "Quick Dipp" stain that I have heard is equivalent, b ut much less expensive.? Or you cxan always use Wright stain or Methylene Blue. Joe Saby, BA HT ________________________________ From: "Feher, Stephen" To: mlbukhar@ucalgary.ca; histonet@lists.utsouthwestern.edu Sent: Wednesday, September 9, 2009 2:50:05 PM Subject: RE: [Histonet] diff-quik stain Thermo Fisher has one called "3 Step Stain"? good stain and if you want to use Methanol as a fixative, rather than the one that would be ordered as a kit, you can save money by only ordering the 3 Step Stain Solution A and 3 Step Stain Solution B.? Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Wednesday, September 09, 2009 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diff-quik stain Does anyone out there in Histonet -land have a recipe to make my own diff-quik stain or a place to buy it. Is it marketed under another name? Thanks ahead, Maureen? Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Sep 9 16:37:24 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Sep 9 16:37:28 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <018b01ca3189$d64a8910$82df9b30$@com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> <018b01ca3189$d64a8910$82df9b30$@com> Message-ID: <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> One of our IT guys heard about it at a conference, that someone had got the Ventana system to print powerpath barcodes on the Ventana. I think it was someone out in Yuma, AZ name Chuy who first did this. The IT guys knew that the interface was transferring the barcode information, but not doing anything with it. After trying for months to talk with someone at Ventana who knew something about this, we finally found him. He then helped our IT dept get it working. The barcode prints right below the normal Ventana barcode. We no longer have the patient name on the slide and concluded that the barcode itself can serve as the second identifier of the specimen. Mark Tarango HT(ASCP)QIHC On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik wrote: > Ventana?s ebar printer ? tell me a little about that, please?. > > > > I don?t think I?m going to like what I hear, but let me shut up and listen > for a little bit. > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 952.241.7369 > > > > > > > > > > *From:* Mark Tarango [mailto:marktarango@gmail.com] > *Sent:* Wednesday, September 09, 2009 3:25 PM > *To:* Michael Mihalik > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Ventana Slide Labels > > > > We currently put the LIS label on the back of the slide. We very recently > got Ventana to help us create a new label template that has the Ventana > barcode and our 2D LIS label all printed on Ventana's Ebar printer. > > > > We will start using the new labels in a few weeks. Ventana doesn't support > the new labels but they did help our IT people figure out how to do it. > > > > Mark Tarango > > On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik > wrote: > > I need some help from those of you who work with Ventana stainers. > > The Ventana equipment requires the use of Ventana slide labels. You can't > just stick your LIS slide label on the slide and expect the Ventana > equipment to read the slide barcode and perform the required stain (yes, I > am assuming an interface is in place). On the other hand, have any of you > either: > > 1. placed the LIS slide label on the back side of the slide, or > 2. Overlapped the LIS slide label with the Ventana slide label such that > you can still read both the LIS and the Ventana barcodes. > > I have heard conflicting answers from multiple Ventana representatives as > to > whether one or both of the aforementioned mechanisms work. > > I appreciate your time. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Rcartun <@t> harthosp.org Wed Sep 9 16:39:33 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Sep 9 16:39:41 2009 Subject: [Histonet] NMDA testing Message-ID: <4AA7E854.7400.0077.1@harthosp.org> Has anyone heard of serum testing for the NMDA receptor associated with "Limbic encephalitis"? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From mike <@t> pathview.com Wed Sep 9 18:44:01 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Sep 9 18:45:05 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> <018b01ca3189$d64a8910$82df9b30$@com> <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> Message-ID: <01d901ca31a7$7bc332d0$73499870$@com> Yeap, news travels fast. I had heard of this as well. For us and our system design, however, the issue is labeling the slide correctly AT THE TIME THE SLIDE IS CUT. I hate that you have to either write patient information on the slide or perform some sort of double labeling. BTW, I assume you have the case # on this slide somewhere, right? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 5:37 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels One of our IT guys heard about it at a conference, that someone had got the Ventana system to print powerpath barcodes on the Ventana. I think it was someone out in Yuma, AZ name Chuy who first did this. The IT guys knew that the interface was transferring the barcode information, but not doing anything with it. After trying for months to talk with someone at Ventana who knew something about this, we finally found him. He then helped our IT dept get it working. The barcode prints right below the normal Ventana barcode. We no longer have the patient name on the slide and concluded that the barcode itself can serve as the second identifier of the specimen. Mark Tarango HT(ASCP)QIHC On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik wrote: Ventana's ebar printer - tell me a little about that, please.. I don't think I'm going to like what I hear, but let me shut up and listen for a little bit. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 3:25 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels We currently put the LIS label on the back of the slide. We very recently got Ventana to help us create a new label template that has the Ventana barcode and our 2D LIS label all printed on Ventana's Ebar printer. We will start using the new labels in a few weeks. Ventana doesn't support the new labels but they did help our IT people figure out how to do it. Mark Tarango On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik wrote: I need some help from those of you who work with Ventana stainers. The Ventana equipment requires the use of Ventana slide labels. You can't just stick your LIS slide label on the slide and expect the Ventana equipment to read the slide barcode and perform the required stain (yes, I am assuming an interface is in place). On the other hand, have any of you either: 1. placed the LIS slide label on the back side of the slide, or 2. Overlapped the LIS slide label with the Ventana slide label such that you can still read both the LIS and the Ventana barcodes. I have heard conflicting answers from multiple Ventana representatives as to whether one or both of the aforementioned mechanisms work. I appreciate your time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From e_dema2000 <@t> yahoo.com Wed Sep 9 19:51:30 2009 From: e_dema2000 <@t> yahoo.com (evette demaisip) Date: Wed Sep 9 19:51:34 2009 Subject: [Histonet] Peloris tissue processor Message-ID: <650420.47113.qm@web44808.mail.sp1.yahoo.com> Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols.?We encountered problems in the?8-hr?and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome.??My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for?feedback/advice on the ff;? 1. Possible reasons? 2.?Steps to prevent this in the future? 3. Reprocessing protocol in Peloris. Thank you so much. Dr. Evette Demaisip Pathologist,Philippines Get your new Email address! Grab the Email name you've always wanted before someone else does! http://mail.promotions.yahoo.com/newdomains/aa/ From Eric.Hoy <@t> UTSouthwestern.edu Wed Sep 9 21:03:25 2009 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Sep 9 21:03:30 2009 Subject: [Histonet] Re: Histonet Digest, Vol 70, Issue 11 In-Reply-To: <200909092345.n89NjgOW009583@nlpi077.prodigy.net> Message-ID: Regarding the LED light sources for fluorescent microscopy, we have been using one of these for the past six months, and it is wonderful. The lighting is bright, even, and it doesn't quench (photobleach) FITC as fast as mercury lamps do. It is easy to align, and the expected life of the LEDs is at least 10,000 hours. I used mercury lamps for many years, and they were fun for those of us who like to tinker with instruments, but overall, they are a pain in the neck to use every day. About two years ago, we bought a new Nikon 50i microscope, which is one of the best clinical microscopes I have ever used. Unfortunately, the Nikon rep talked us into the EXFO metal halide light source. This looked good in the demo, but it has spent more time being repaired than working in our lab. Right now it is back at Nikon while they try to figure out what is wrong with it. The LED light source that we are currently using is from a company called Fraen. They are an Italian company, but they have an office in the US. The unit is small, easy to install, easy to align, and gives illumination equivalent to a 100 watt mercury lamp. Merced said that there are no LEDs available for red wavelengths, but this must just be true for Zeiss. Fraen has LED modules that cover virtually all of the fluorochromes that I have ever heard of. They have one for red wavelengths that will work with TRITC, Cy3, Texas Red, Alexa Fluor 633, Alexa Fluor 647, and even Cy5, which has maximum excitation at about 650 nm, well into the red range. You do need to have different LEDs for various fluorochromes, but Fraen has 7 modules available, and for practical terms, about 4 of them would cover everything from DAPI to Cy5. They are easy to interchange on the Fraen unit. I don't work for any of the companies mentioned here, nor do I receive any compensation for saying nice things about their products. I just like their products. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== > Message: 1 > Date: Wed, 09 Sep 2009 13:43:45 -0400 > From: Merced M Leiker > Subject: Re: [Histonet] mercury vs led lights > To: Nejat Yilmaz , > histonet@lists.utsouthwestern.edu > Message-ID: <6276F2A000238523BC2681F5@CDYwxp1931.ad.med.buffalo.edu> > Content-Type: text/plain; charset=us-ascii; format=flowed > > I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 > (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor > 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted > microscope we have LEDs and we also have a mercury lamp to cover the red > range. The Zeiss switches nicely and very smoothly between the light > sources during viewing and image acquisition. > > Sorry for the highly untechnical description, but that is all I know about > it. :-) From Eric.Hoy <@t> UTSouthwestern.edu Wed Sep 9 21:56:58 2009 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Sep 9 21:57:02 2009 Subject: [Histonet] Dictation software Message-ID: I have been using the Dragon software for the Mac (MacSpeech Dictate) for about six months. Unlike Dr. Richmond, my typing skills leave a lot to be desired. Sometimes I?ll get three fingers involved in the process, but I?m getting pretty reckless when I try typing that fast. About a month ago, I got the medical version of the MacSpeech Dictate, and it is even better than the regular edition. It has no trouble with terms like polyacrylamide gel electrophoresis, reverse transcriptase polymerase chain reaction, and other terms that immunologists use. Certain abbreviations might confuse it, like ANCA (pronounced like Paul Anka, but the software makes it ?anchor?), but it has no trouble with antineutrophil cytoplasmic autoantibodies. It is possible to dictate in ?spelling mode? and pronounce each letter to get abbreviations. It took me about five minutes to train the software to recognize my voice, and about a month to train me to dictate properly. It works a lot better with a complete sentences than individual words. I?m not a pathologist, so I don?t use a lot of the terms that they use, but for my purposes, this software works well. I dictated this entire message, except for the signature block, using the software. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== From baranowski <@t> mindspring.com Thu Sep 10 04:23:00 2009 From: baranowski <@t> mindspring.com (Tom & Cindy Baranowski) Date: Thu Sep 10 04:23:03 2009 Subject: [Histonet] Richard Allan Xylene Message-ID: We've changed our primary vendor and I just received a shipment of Richard Allan Xylene. I've never used their brand of xylene before (used their stains for years) we've always used the name brand from another major scientific vendor. Has anyone had any problems with the RA xylene? We will be using it for tissue processing and in our stain setup. I appreciate any comments pro or con. Cindy Baranowski SJTRI Atlanta, GA From vonavi <@t> inbox.lv Thu Sep 10 05:45:17 2009 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Thu Sep 10 05:45:24 2009 Subject: [Histonet] Blood for confocal microscopy Message-ID: <1252579517.4aa8d8bd23558@mail.inbox.lv> Hello, I would like to do confocal microscopy of human blood cells (blood smear). Could anyone please share his/her robustly working protocol? Thank you very much in advance. Andrey From rjbuesa <@t> yahoo.com Thu Sep 10 08:11:52 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 10 08:11:56 2009 Subject: [Histonet] Richard Allan Xylene In-Reply-To: Message-ID: <112457.15312.qm@web65707.mail.ac4.yahoo.com> None of these vendors manufacture xylene, they all distribute it and probably get theirs from the same petroleum distillery, so fear not about possible differences. Ren? J. --- On Thu, 9/10/09, Tom & Cindy Baranowski wrote: From: Tom & Cindy Baranowski Subject: [Histonet] Richard Allan Xylene To: histonet@lists.utsouthwestern.edu Date: Thursday, September 10, 2009, 5:23 AM We've changed our primary vendor and I just received a shipment of Richard Allan Xylene.? I've never used their brand of xylene before (used their stains for years) we've always used the name brand from another major scientific vendor.? Has anyone had any problems with the RA xylene?? We will be using it for tissue processing and in our stain setup.? I appreciate any comments pro or con. Cindy Baranowski SJTRI Atlanta, GA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 10 08:12:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 10 08:12:50 2009 Subject: [Histonet] Peloris tissue processor In-Reply-To: <650420.47113.qm@web44808.mail.sp1.yahoo.com> Message-ID: <9777.84492.qm@web65710.mail.ac4.yahoo.com> Your problems probably are caused by the dehydration steps. Check them Ren? J. --- On Wed, 9/9/09, evette demaisip wrote: From: evette demaisip Subject: [Histonet] Peloris tissue processor To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 9, 2009, 8:51 PM Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols.?We encountered problems in the?8-hr?and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome.??My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for?feedback/advice on the ff;? 1. Possible reasons? 2.?Steps to prevent this in the future? 3. Reprocessing protocol in Peloris. Thank you so much. Dr. Evette Demaisip Pathologist,Philippines ? ? ? Get your new Email address! Grab the Email name you've always wanted before someone else does! http://mail.promotions.yahoo.com/newdomains/aa/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Sep 10 09:21:59 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Sep 10 09:22:04 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <01d901ca31a7$7bc332d0$73499870$@com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> <018b01ca3189$d64a8910$82df9b30$@com> <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> <01d901ca31a7$7bc332d0$73499870$@com> Message-ID: <5b6eb13e0909100721u549c24e5qbad30072649f5609@mail.gmail.com> Oh yah, we have the case number on there. Using these labels, we can put the label on before the section is cut and just use that single label for everything. Aside from doing the same thing (getting labels with both barcodes), I can't think of a way around writing on the slides or double labeling. Mark Tarango On Wed, Sep 9, 2009 at 4:44 PM, Michael Mihalik wrote: > Yeap, news travels fast. I had heard of this as well. > > > > For us and our system design, however, the issue is labeling the slide > correctly AT THE TIME THE SLIDE IS CUT. I hate that you have to either > write patient information on the slide or perform some sort of double > labeling. > > > > BTW, I assume you have the case # on this slide somewhere, right? > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 952.241.7369 > > > > > > > > > > *From:* Mark Tarango [mailto:marktarango@gmail.com] > *Sent:* Wednesday, September 09, 2009 5:37 PM > > *To:* Michael Mihalik > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Ventana Slide Labels > > > > One of our IT guys heard about it at a conference, that someone had got the > Ventana system to print powerpath barcodes on the Ventana. I think it was > someone out in Yuma, AZ name Chuy who first did this. > > > > The IT guys knew that the interface was transferring the barcode > information, but not doing anything with it. After trying for months to > talk with someone at Ventana who knew something about this, we finally found > him. He then helped our IT dept get it working. > > > > The barcode prints right below the normal Ventana barcode. We no longer > have the patient name on the slide and concluded that the barcode itself can > serve as the second identifier of the specimen. > > > > Mark Tarango HT(ASCP)QIHC > > > > > > > > > > On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik wrote: > > Ventana?s ebar printer ? tell me a little about that, please?. > > > > I don?t think I?m going to like what I hear, but let me shut up and listen > for a little bit. > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 952.241.7369 > > > > > > > > > > *From:* Mark Tarango [mailto:marktarango@gmail.com] > *Sent:* Wednesday, September 09, 2009 3:25 PM > *To:* Michael Mihalik > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Ventana Slide Labels > > > > We currently put the LIS label on the back of the slide. We very recently > got Ventana to help us create a new label template that has the Ventana > barcode and our 2D LIS label all printed on Ventana's Ebar printer. > > > > We will start using the new labels in a few weeks. Ventana doesn't support > the new labels but they did help our IT people figure out how to do it. > > > > Mark Tarango > > On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik > wrote: > > I need some help from those of you who work with Ventana stainers. > > The Ventana equipment requires the use of Ventana slide labels. You can't > just stick your LIS slide label on the slide and expect the Ventana > equipment to read the slide barcode and perform the required stain (yes, I > am assuming an interface is in place). On the other hand, have any of you > either: > > 1. placed the LIS slide label on the back side of the slide, or > 2. Overlapped the LIS slide label with the Ventana slide label such that > you can still read both the LIS and the Ventana barcodes. > > I have heard conflicting answers from multiple Ventana representatives as > to > whether one or both of the aforementioned mechanisms work. > > I appreciate your time. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From mike <@t> pathview.com Thu Sep 10 10:26:35 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Sep 10 10:27:28 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <5b6eb13e0909100721u549c24e5qbad30072649f5609@mail.gmail.com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> <018b01ca3189$d64a8910$82df9b30$@com> <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> <01d901ca31a7$7bc332d0$73499870$@com> <5b6eb13e0909100721u549c24e5qbad30072649f5609@mail.gmail.com> Message-ID: <026b01ca322b$238df990$6aa9ecb0$@com> So can you explain your layout a little bit, please? At the microtome, do you have a Ventana slide label printer and an LIS slide labeler? Put it another way: You received the request for an IHC. Now, what happens?... with emphasis on the labeling? Thank you again for your time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Thursday, September 10, 2009 10:22 AM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels Oh yah, we have the case number on there. Using these labels, we can put the label on before the section is cut and just use that single label for everything. Aside from doing the same thing (getting labels with both barcodes), I can't think of a way around writing on the slides or double labeling. Mark Tarango On Wed, Sep 9, 2009 at 4:44 PM, Michael Mihalik wrote: Yeap, news travels fast. I had heard of this as well. For us and our system design, however, the issue is labeling the slide correctly AT THE TIME THE SLIDE IS CUT. I hate that you have to either write patient information on the slide or perform some sort of double labeling. BTW, I assume you have the case # on this slide somewhere, right? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 5:37 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels One of our IT guys heard about it at a conference, that someone had got the Ventana system to print powerpath barcodes on the Ventana. I think it was someone out in Yuma, AZ name Chuy who first did this. The IT guys knew that the interface was transferring the barcode information, but not doing anything with it. After trying for months to talk with someone at Ventana who knew something about this, we finally found him. He then helped our IT dept get it working. The barcode prints right below the normal Ventana barcode. We no longer have the patient name on the slide and concluded that the barcode itself can serve as the second identifier of the specimen. Mark Tarango HT(ASCP)QIHC On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik wrote: Ventana's ebar printer - tell me a little about that, please.. I don't think I'm going to like what I hear, but let me shut up and listen for a little bit. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 3:25 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels We currently put the LIS label on the back of the slide. We very recently got Ventana to help us create a new label template that has the Ventana barcode and our 2D LIS label all printed on Ventana's Ebar printer. We will start using the new labels in a few weeks. Ventana doesn't support the new labels but they did help our IT people figure out how to do it. Mark Tarango On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik wrote: I need some help from those of you who work with Ventana stainers. The Ventana equipment requires the use of Ventana slide labels. You can't just stick your LIS slide label on the slide and expect the Ventana equipment to read the slide barcode and perform the required stain (yes, I am assuming an interface is in place). On the other hand, have any of you either: 1. placed the LIS slide label on the back side of the slide, or 2. Overlapped the LIS slide label with the Ventana slide label such that you can still read both the LIS and the Ventana barcodes. I have heard conflicting answers from multiple Ventana representatives as to whether one or both of the aforementioned mechanisms work. I appreciate your time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu Sep 10 10:46:53 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Sep 10 10:46:58 2009 Subject: [Histonet] HT Part Time Job/Friday-8hrs/week Message-ID: Hello Histonetters, One of my clients has asked me to do a favor and spread the word about a Part Time histotechnician/histotechnologist position in Altamonte Springs, FL with a private laboratory. Please let me know if you or anyone that you know would be interested in the following position: Position: Histotechnologist/Histotechnician Time: 9am-1am Days: Fridays Only Requirments: ASCP, Degree preferred Facility: Private Derm Lab Location: Altamonte Springs, FL Please submit resume to alyssa@alliedsearchpartners.com for initial prescreening, and one of our recruiters will follow up with a phone screen/interview after receiving your resume. Have a great day! -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From marktarango <@t> gmail.com Thu Sep 10 11:53:59 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Sep 10 11:54:04 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <026b01ca322b$238df990$6aa9ecb0$@com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> <018b01ca3189$d64a8910$82df9b30$@com> <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> <01d901ca31a7$7bc332d0$73499870$@com> <5b6eb13e0909100721u549c24e5qbad30072649f5609@mail.gmail.com> <026b01ca322b$238df990$6aa9ecb0$@com> Message-ID: <5b6eb13e0909100953h615e3965t5f42c2ef55476d81@mail.gmail.com> The tech checks the worklist in powerpath for any orders. The worklist is then printed. He/she goes to the Ventana instrument to print the labels that have transferred over through the interface. Then they take the labels to the control slide area and grab their pre-cut control slides and put the labels on the slides. Then they go over to the waterbath and cut the orders. Since we only have two location where Ventana labels can be printed, the tech has to get up and to go print them. At the cutting station there is only a slide label printer for LIS/Powerpath labels. Mark Tarango On Thu, Sep 10, 2009 at 8:26 AM, Michael Mihalik wrote: > So can you explain your layout a little bit, please? At the microtome, > do you have a Ventana slide label printer and an LIS slide labeler? > > > > Put it another way: You received the request for an IHC. Now, what > happens?... with emphasis on the labeling? > > > > > > Thank you again for your time. > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 952.241.7369 > > > > > > > > > > *From:* Mark Tarango [mailto:marktarango@gmail.com] > *Sent:* Thursday, September 10, 2009 10:22 AM > > *To:* Michael Mihalik > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Ventana Slide Labels > > > > Oh yah, we have the case number on there. Using these labels, we can put > the label on before the section is cut and just use that single label for > everything. > > > > Aside from doing the same thing (getting labels with both barcodes), I > can't think of a way around writing on the slides or double labeling. > > Mark Tarango > > > > > On Wed, Sep 9, 2009 at 4:44 PM, Michael Mihalik wrote: > > Yeap, news travels fast. I had heard of this as well. > > > > For us and our system design, however, the issue is labeling the slide > correctly AT THE TIME THE SLIDE IS CUT. I hate that you have to either > write patient information on the slide or perform some sort of double > labeling. > > > > BTW, I assume you have the case # on this slide somewhere, right? > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 952.241.7369 > > > > > > > > > > *From:* Mark Tarango [mailto:marktarango@gmail.com] > *Sent:* Wednesday, September 09, 2009 5:37 PM > > > *To:* Michael Mihalik > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Ventana Slide Labels > > > > One of our IT guys heard about it at a conference, that someone had got the > Ventana system to print powerpath barcodes on the Ventana. I think it was > someone out in Yuma, AZ name Chuy who first did this. > > > > The IT guys knew that the interface was transferring the barcode > information, but not doing anything with it. After trying for months to > talk with someone at Ventana who knew something about this, we finally found > him. He then helped our IT dept get it working. > > > > The barcode prints right below the normal Ventana barcode. We no longer > have the patient name on the slide and concluded that the barcode itself can > serve as the second identifier of the specimen. > > > > Mark Tarango HT(ASCP)QIHC > > > > > > > > > > On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik wrote: > > Ventana?s ebar printer ? tell me a little about that, please?. > > > > I don?t think I?m going to like what I hear, but let me shut up and listen > for a little bit. > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 952.241.7369 > > > > > > > > > > *From:* Mark Tarango [mailto:marktarango@gmail.com] > *Sent:* Wednesday, September 09, 2009 3:25 PM > *To:* Michael Mihalik > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Ventana Slide Labels > > > > We currently put the LIS label on the back of the slide. We very recently > got Ventana to help us create a new label template that has the Ventana > barcode and our 2D LIS label all printed on Ventana's Ebar printer. > > > > We will start using the new labels in a few weeks. Ventana doesn't support > the new labels but they did help our IT people figure out how to do it. > > > > Mark Tarango > > On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik > wrote: > > I need some help from those of you who work with Ventana stainers. > > The Ventana equipment requires the use of Ventana slide labels. You can't > just stick your LIS slide label on the slide and expect the Ventana > equipment to read the slide barcode and perform the required stain (yes, I > am assuming an interface is in place). On the other hand, have any of you > either: > > 1. placed the LIS slide label on the back side of the slide, or > 2. Overlapped the LIS slide label with the Ventana slide label such that > you can still read both the LIS and the Ventana barcodes. > > I have heard conflicting answers from multiple Ventana representatives as > to > whether one or both of the aforementioned mechanisms work. > > I appreciate your time. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > From ryaskovich <@t> dir.nidcr.nih.gov Thu Sep 10 12:02:47 2009 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Thu Sep 10 12:02:55 2009 Subject: [Histonet] LFB staining with Immuno. Staining Message-ID: I'm looking for the person who posted this information (I lost the email). I want to stain human and non-human primate ganglia with the TRPV-1 and use the LFB as a counter stain. Any help is greatly appreciated. Thanks Ruth N.I.H. From jely <@t> mdanderson.org Thu Sep 10 12:08:54 2009 From: jely <@t> mdanderson.org (Ely,Jeanenne C) Date: Thu Sep 10 12:08:57 2009 Subject: [Histonet] Aurora A IHC in mouse tissue Message-ID: Dear Histonet: Have any of you had success with any antibodies to Aurora A in ffpe mouse tissues? I have tried the mouse monoclonal from Novocastra (NCL-L-AK2) using a MOM kit, with not much luck. Any suggestions? Thanks, Jeanenne Ely, BS, HT(ASCP), QIHC MD Anderson Cancer Center Veterinary Medicine & Surgery Unit 63 1515 Holcombe Blvd, Houston, TX 77030 (713) 792-2793 From rsrichmond <@t> gmail.com Thu Sep 10 12:28:16 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Sep 10 12:28:21 2009 Subject: [Histonet] Re: Richard Allan Xylene Message-ID: Unlike the aliphatic hydrocarbons, I would not expect different brands of xylene to differ from each other. Xylene is dimethylbenzene, which has three possible isomers (ortho, meta, and para). Commercial xylene is a mixture of the three isomers, with some ethylbenzene present also. (That's why it's labeled "xylenes" rather than "xylene".) The isomers differ slightly from each other in physical and chemical properties, but I doubt that the differences would be noticeable in the histology lab. The company is spelled "Richard-Allan", commonly misspelled. Bob Richmond Samurai Pathologist Knoxville TN From mike <@t> pathview.com Thu Sep 10 12:28:43 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Sep 10 12:29:37 2009 Subject: [Histonet] Ventana Slide Labels In-Reply-To: <5b6eb13e0909100953h615e3965t5f42c2ef55476d81@mail.gmail.com> References: <00c401ca317a$45a2d1b0$d0e87510$@ca> <73A7ED895EE0C24D9267ED814911DF190FDB1E75@exchange.cmc-nh.org> <016401ca3182$6c086740$441935c0$@com> <5b6eb13e0909091224p7299efd0v84271c9cd701cea7@mail.gmail.com> <018b01ca3189$d64a8910$82df9b30$@com> <5b6eb13e0909091437g700d6c26q87bed2db4f10bbf5@mail.gmail.com> <01d901ca31a7$7bc332d0$73499870$@com> <5b6eb13e0909100721u549c24e5qbad30072649f5609@mail.gmail.com> <026b01ca322b$238df990$6aa9ecb0$@com> <5b6eb13e0909100953h615e3965t5f42c2ef55476d81@mail.gmail.com> Message-ID: <02c701ca323c$3446c440$9cd44cc0$@com> Ahh, that's what I wanted to hear. Thank you very much. I would also like to thank everyone else who has responded. In summary, at least a couple of people have found out how to print their LIS barcodes on the Ventana label. This is done via an interface and the printer is the printer connected to the Ventana PC. While this is a very good improvement, and I compliment Jesus Ellin on his work down in Yuma (got to give credit to those who have earned it), I yearn for the time when a generic slide label and barcode can be read by the Ventana gear. When that happens, we can label the slides like all the other slides - one at a time, while the material is being cut. Again, thank you all very much. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Thursday, September 10, 2009 12:54 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels The tech checks the worklist in powerpath for any orders. The worklist is then printed. He/she goes to the Ventana instrument to print the labels that have transferred over through the interface. Then they take the labels to the control slide area and grab their pre-cut control slides and put the labels on the slides. Then they go over to the waterbath and cut the orders. Since we only have two location where Ventana labels can be printed, the tech has to get up and to go print them. At the cutting station there is only a slide label printer for LIS/Powerpath labels. Mark Tarango On Thu, Sep 10, 2009 at 8:26 AM, Michael Mihalik wrote: So can you explain your layout a little bit, please? At the microtome, do you have a Ventana slide label printer and an LIS slide labeler? Put it another way: You received the request for an IHC. Now, what happens?... with emphasis on the labeling? Thank you again for your time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Thursday, September 10, 2009 10:22 AM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels Oh yah, we have the case number on there. Using these labels, we can put the label on before the section is cut and just use that single label for everything. Aside from doing the same thing (getting labels with both barcodes), I can't think of a way around writing on the slides or double labeling. Mark Tarango On Wed, Sep 9, 2009 at 4:44 PM, Michael Mihalik wrote: Yeap, news travels fast. I had heard of this as well. For us and our system design, however, the issue is labeling the slide correctly AT THE TIME THE SLIDE IS CUT. I hate that you have to either write patient information on the slide or perform some sort of double labeling. BTW, I assume you have the case # on this slide somewhere, right? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 5:37 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels One of our IT guys heard about it at a conference, that someone had got the Ventana system to print powerpath barcodes on the Ventana. I think it was someone out in Yuma, AZ name Chuy who first did this. The IT guys knew that the interface was transferring the barcode information, but not doing anything with it. After trying for months to talk with someone at Ventana who knew something about this, we finally found him. He then helped our IT dept get it working. The barcode prints right below the normal Ventana barcode. We no longer have the patient name on the slide and concluded that the barcode itself can serve as the second identifier of the specimen. Mark Tarango HT(ASCP)QIHC On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik wrote: Ventana's ebar printer - tell me a little about that, please.. I don't think I'm going to like what I hear, but let me shut up and listen for a little bit. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Wednesday, September 09, 2009 3:25 PM To: Michael Mihalik Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Slide Labels We currently put the LIS label on the back of the slide. We very recently got Ventana to help us create a new label template that has the Ventana barcode and our 2D LIS label all printed on Ventana's Ebar printer. We will start using the new labels in a few weeks. Ventana doesn't support the new labels but they did help our IT people figure out how to do it. Mark Tarango On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik wrote: I need some help from those of you who work with Ventana stainers. The Ventana equipment requires the use of Ventana slide labels. You can't just stick your LIS slide label on the slide and expect the Ventana equipment to read the slide barcode and perform the required stain (yes, I am assuming an interface is in place). On the other hand, have any of you either: 1. placed the LIS slide label on the back side of the slide, or 2. Overlapped the LIS slide label with the Ventana slide label such that you can still read both the LIS and the Ventana barcodes. I have heard conflicting answers from multiple Ventana representatives as to whether one or both of the aforementioned mechanisms work. I appreciate your time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Sep 10 13:14:00 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Sep 10 13:14:05 2009 Subject: [Histonet] Double Staining Message-ID: Hi All - Just wanted to share some information that I was happy to come across regarding double staining FFPE tissues. DBS (dbiosys.com) has a great variety of products that are stable and have consistent bright results. The pricing is not that bad either. They have mouse and rabbit HRP and Alk Phos with the corresponding chromogens. Which makes your double staining possibilities endless. I have tried several companies double staining kits and this one has to be the best so far. I have been using their PIN 4 (p63/HMW/p504s) with HRP/DAB and AP/PermaRed with great results. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology From tfountain <@t> exchange.hsc.mb.ca Thu Sep 10 13:57:09 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Thu Sep 10 13:58:33 2009 Subject: [Histonet] Dako Tubing Message-ID: Hi all, We are looking for the tubing that is used for pumping the bulk buffers and the waste. We don't want to order the tube and cap assembly from DAKO (cost saving measure) and this Tygon R 3606 seems to be available from VWR however, I am not sure of the diameter/specs of the tubing and our Dako rep said he didn't know. Is anyone currently using something other than the one from DAKO? Thank you for any help. Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From lchen <@t> mednet.ucla.edu Thu Sep 10 15:03:32 2009 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Thu Sep 10 15:03:56 2009 Subject: [Histonet] Permount question Message-ID: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> Hello, Did anyone ever use Permount straight without cutting it with some solvent? Journal articles mention using Permount to coverslip, and the post-doc I work with thinks that it isn't diluted. I think maybe people just universally "know" that it should be diluted so it isn't mentioned in the Materials and Methods. Any thoughts? Leslie From Maria.Katleba <@t> stjoe.org Thu Sep 10 15:07:14 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Sep 10 15:07:30 2009 Subject: [Histonet] Histotech Position FT Position available ASAP NAPA CA Message-ID: <97C02552ECB11346877D3E83CF833ABD13D55A60F7@SJSNT-SCMAIL03.stjoe.org> Full Time Histotech position 3am shift (YES!!! Please be aware this is an early shift) Located in Northern California's Napa Valley, home of California best vineyards. Quiet, clean town. Very safe and family friendly. Some of the best private and public schools just blocks away from the hospital. Benefitted, PTO accrual, Dental / Vision/ and Medical, etc SERIOUS APPLICANTS ONLY- Do not apply if you aren't sure of the shift or if you might change your mind. We want to hire someone who wants to be a productive individual Workload ranges from 40-80 blocks sometimes more, sometimes less 40 hours week. Monday thru Friday .... Ventana Benchmark XT for IHC & Ventana Nexus for Special stains (Few manual stains) Automated stainer, Leica Tissue Processor Apply at www.thequeen.org , fax your resume (707) 257-4133 attn: Maria, or call me. Looking for an employee who wants to work and contribute to their department. Applicant must take direction, as well as be self motivated. Licensed applicants preferred (or ability to take the exam). Experience is necessary. Any questions, call me between 9am and 3pm Monday to Friday. Education preferred. Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From talulahgosh <@t> gmail.com Thu Sep 10 15:10:50 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Sep 10 15:10:56 2009 Subject: [Histonet] Permount question In-Reply-To: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> References: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> Message-ID: I've never heard of having to dilute it--we just use it straight. Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Thu, Sep 10, 2009 at 4:03 PM, Leslie Chen wrote: > Hello, > Did anyone ever use Permount straight without cutting it with some solvent? > Journal articles mention using Permount to coverslip, and the post-doc I > work with thinks that it isn't diluted. > > I think maybe people just universally "know" that it should be diluted so > it > isn't mentioned in the Materials and Methods. Any thoughts? > > Leslie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Thu Sep 10 15:18:46 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Sep 10 15:17:10 2009 Subject: [Histonet] Permount question In-Reply-To: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> References: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> Message-ID: <4AA95F26.5030104@umdnj.edu> If someone leaves the cap off and some of the solvent evaporates you will have to restore it. Otherwise, straight from the bottle. Bu the way, DPX is better in many ways. Geoff Leslie Chen wrote: > Hello, > Did anyone ever use Permount straight without cutting it with some solvent? > Journal articles mention using Permount to coverslip, and the post-doc I > work with thinks that it isn't diluted. > > I think maybe people just universally "know" that it should be diluted so it > isn't mentioned in the Materials and Methods. Any thoughts? > > Leslie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From leiker <@t> buffalo.edu Thu Sep 10 15:28:11 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Sep 10 15:28:18 2009 Subject: [Histonet] Permount question In-Reply-To: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> References: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> Message-ID: <444A01A40B696E8413C711E5@CDYwxp1931.ad.med.buffalo.edu> I use it straight out of the bottle. My supervisor uses it straight. The institution where I first learned to coverslip used it straight as well. I'm pretty sure the UB Histology Lab here uses it straight as well... Merced --On Thursday, September 10, 2009 1:03 PM -0700 Leslie Chen wrote: > Hello, > Did anyone ever use Permount straight without cutting it with some > solvent? Journal articles mention using Permount to coverslip, and the > post-doc I work with thinks that it isn't diluted. > > I think maybe people just universally "know" that it should be diluted so > it isn't mentioned in the Materials and Methods. Any thoughts? > > Leslie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From carrolpb <@t> umdnj.edu Thu Sep 10 15:36:11 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Sep 10 15:36:24 2009 Subject: [Histonet] Permount question In-Reply-To: <444A01A40B696E8413C711E5@CDYwxp1931.ad.med.buffalo.edu> References: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> <444A01A40B696E8413C711E5@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <4AA9633B.8040505@umdnj.edu> We use it straight here, as well... Merced M Leiker wrote: > I use it straight out of the bottle. My supervisor uses it straight. > The institution where I first learned to coverslip used it straight as > well. I'm pretty sure the UB Histology Lab here uses it straight as > well... > > Merced > > --On Thursday, September 10, 2009 1:03 PM -0700 Leslie Chen > wrote: > >> Hello, >> Did anyone ever use Permount straight without cutting it with some >> solvent? Journal articles mention using Permount to coverslip, and the >> post-doc I work with thinks that it isn't diluted. >> >> I think maybe people just universally "know" that it should be >> diluted so >> it isn't mentioned in the Materials and Methods. Any thoughts? >> >> Leslie >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jstaruk <@t> masshistology.com Thu Sep 10 15:46:38 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Thu Sep 10 15:46:33 2009 Subject: [Histonet] Polarizing filters In-Reply-To: Message-ID: Does anyone know where I can find the two appropriate filters (lenses) needed to polarize the congo red and Sirius red stains? I have an Olympus CH-2 that needs to be fitted. I understand I need a "polarizer" lens and an "analyzer" lens. Are these two different lenses or the same lens, just in different locations on the microscope? Thank you Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From lchen <@t> mednet.ucla.edu Thu Sep 10 15:52:37 2009 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Thu Sep 10 15:53:00 2009 Subject: [Histonet] Re: Permount question In-Reply-To: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> References: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> Message-ID: <361429220909101352w748222cs10e4e5f27796440@mail.gmail.com> Thanks for all the replies, I guess these weird Asian technicians who are highly looked up to taught me something wrong and it's been propagated in the department. Thanks again! On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen wrote: > Hello, > Did anyone ever use Permount straight without cutting it with some > solvent? Journal articles mention using Permount to coverslip, and the > post-doc I work with thinks that it isn't diluted. > > I think maybe people just universally "know" that it should be diluted so > it isn't mentioned in the Materials and Methods. Any thoughts? > > Leslie > From LSebree <@t> uwhealth.org Thu Sep 10 16:00:11 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Sep 10 16:00:18 2009 Subject: [Histonet] Ventana users only Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A589035706AF@UWHC-MAIL01.uwhis.hosp.wisc.edu> An unscientific poll wondering: How many Ventana customers have made the switch from iView DAB detection to Ultra View DAB? Why? What kind of differences did you see in staining between the two detections. How was the additional cost of Ultra View justified? Did being able to drop the biotin blocking step make it worth the switch? What percentage of antibodies were you able to go to a weaker dilution? Shorter incubation time? What percentage of protocols were you able to lessen retrieval, i.e. standard CC1 to mild CC1? Any information is welcome or call/e-mail me directly. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From trathborne <@t> somerset-healthcare.com Thu Sep 10 16:02:50 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Sep 10 16:02:57 2009 Subject: [Histonet] Ventana users only In-Reply-To: <5998F3BDFF7AAC4091C7AE93A7A1A589035706AF@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: I would like to see these answers on Histonet. Please reply to all. Thanks, Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A Sent: Thursday, September 10, 2009 5:00 PM To: Histonet Subject: [Histonet] Ventana users only An unscientific poll wondering: How many Ventana customers have made the switch from iView DAB detection to Ultra View DAB? Why? What kind of differences did you see in staining between the two detections. How was the additional cost of Ultra View justified? Did being able to drop the biotin blocking step make it worth the switch? What percentage of antibodies were you able to go to a weaker dilution? Shorter incubation time? What percentage of protocols were you able to lessen retrieval, i.e. standard CC1 to mild CC1? Any information is welcome or call/e-mail me directly. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From JCordova <@t> omlabs.com Thu Sep 10 16:16:53 2009 From: JCordova <@t> omlabs.com (Cordova, Jean) Date: Thu Sep 10 16:17:17 2009 Subject: [Histonet] Slide labels used with IHC Instruments Message-ID: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. From mike <@t> pathview.com Thu Sep 10 16:40:04 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Sep 10 16:41:00 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> Message-ID: <035101ca325f$54fe3bf0$fefab3d0$@com> Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu Sep 10 18:32:57 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Sep 10 18:33:02 2009 Subject: [Histonet] Job Upstate NY Message-ID: Good Evening Histonetters! Allied Search Partners is now accepting resumes for the following position(s) in Round Lake/Saratoga Springs NY area. TITLE/POSITION Status: Exempt. Regular, full-time. Histotechnologist/Histotechnician Work Shift: Monday-Friday, Mid Morning Shift about 11am-7pm (Hours are flexible.) QUALIFICATIONS ASCP certification required Prior experience working as a histotech a must Degree preferred NY licensed Be sure to submit your resume to alyssa@alliedsearchpartners.com for review and prescreening. *All inquiries are kept confidential* and one of our recruiters will call for an initial phone screen. Thank you! *Be sure to visit us on the web* www.alliedsearchpartners.com to submit a referral for a cash bonus, submit a job request, or contact one of our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From milton.gomez <@t> aruplab.com Thu Sep 10 21:35:45 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Thu Sep 10 21:36:22 2009 Subject: [Histonet] Ventana ULTRA Immuno Stainer Message-ID: Dear Histonetters, Is anyone out there using the Ventana ULTRA Immuno Stainer? What is your experience with them? What are the advantages, disadvantages, pros and cons? Thank you very much in advance? Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.gomez@aruplab.com Web: www.aruplab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From CPhipps <@t> cblpath.com Thu Sep 10 22:32:24 2009 From: CPhipps <@t> cblpath.com (Carlene K. Phipps) Date: Thu Sep 10 22:32:39 2009 Subject: [Histonet] (no subject) Message-ID: <0EBA2D55BAF71C4984EF84CC969C77CA050A9C13@OcalaExchange.cblpath.local> Hi All, This is my first time on Histonet. Does anyone have information on FISH oncology processing? Specifically, the preparation of the slide from bone marrow or peripheral blood? Also information of equipment needs excluding the VP-2000 and the thermobrite, would be appreciated. Thanks, Carlene Phipps, BA,HT, ASCP(QIHC) CBLPath, Inc From Janice.Mahoney <@t> alegent.org Fri Sep 11 08:13:50 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Sep 11 08:13:58 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <035101ca325f$54fe3bf0$fefab3d0$@com> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Rebecca.Riesen <@t> nchmd.org Fri Sep 11 08:15:47 2009 From: Rebecca.Riesen <@t> nchmd.org (Riesen, Rebecca) Date: Fri Sep 11 08:15:54 2009 Subject: [Histonet] formalin storage Message-ID: We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. From MSHERWOOD <@t> PARTNERS.ORG Fri Sep 11 08:22:31 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Sep 11 08:22:37 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23CB8@PHSXMB30.partners.org> We don't. We buy a 5-gallon cubitainer which sits on a counter. First, it would be inconvenient to the users to have to constantly go into the flammable cabinet to access. Second, it comes in a cardboard container which is not allowed in a flammable cabinet (per Fire Department). We have an outside hazardous company, plus regular visits by the Fire Department and no one has mentioned keeping it in the flammable cabinet. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Friday, September 11, 2009 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From jwarren23 <@t> cinci.rr.com Fri Sep 11 08:25:03 2009 From: jwarren23 <@t> cinci.rr.com (Jean Warren) Date: Fri Sep 11 08:25:36 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Fri Sep 11 08:25:41 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Fri Sep 11 08:25:45 2009 Subject: [Histonet] Errors in the lab Message-ID: <50837.52197.qm@web65708.mail.ac4.yahoo.com> I was just wondering how others are tracking mistakes.? We have a form that the employees fill out when they catch and rersolve mistakes.? I feel this gives me the opportunity to recognize problem areas (or people).? I was approached by one of my staff who feels that this may take away from a "team approach" and makes people feel picked on.? On one hand I can see that point, but the error forms are only seen by the person catching the error and me.? I guess I was wondering what everyone thinks? ? Thanks. From Jackie.O'Connor <@t> abbott.com Fri Sep 11 08:31:13 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 11 08:31:40 2009 Subject: [Histonet] formalin storage In-Reply-To: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> Message-ID: I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. "Jean Warren" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/11/2009 08:25 AM To "Riesen, Rebecca" , cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Sep 11 08:31:04 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Sep 11 08:31:43 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> Message-ID: <003d01ca32e4$2d4ffeb0$87effc10$@com> Janice, may I ask whom you have for your LIS? Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 9:14 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From talulahgosh <@t> gmail.com Fri Sep 11 08:34:03 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Sep 11 08:34:09 2009 Subject: [Histonet] formalin storage In-Reply-To: References: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> Message-ID: I have to ask--what was the point of chain mail gloves?! Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Fri, Sep 11, 2009 at 9:31 AM, Jackie M O'Connor < Jackie.O'Connor@abbott.com > wrote: > I once had my safety officer insist I wear chain maille gloves while > cutting frozen sections. They didn' t care about all the reasons I gave > them why I shouldn't - like it would be impossible to use the machine > while wearing them, and the patient would have to lie on the operating > table longer waiting to find out if their entire colon was going to be > removed. > > > > "Jean Warren" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 09/11/2009 08:25 AM > > To > "Riesen, Rebecca" , > > cc > > Subject > Re: [Histonet] formalin storage > > > > > > > No, it is ridiculous. Safety people tried to argue this with us years ago. > > One of our pathologists told them, "How can something that is almost 90% > water be a combustion hazard?" > > > ----- Original Message ----- > From: "Riesen, Rebecca" > To: > Sent: Friday, September 11, 2009 9:15 AM > Subject: [Histonet] formalin storage > > > > > We have been directed by our Safety Officer to store all formalin (37% > and 10% NBF) in a flammable storage room, cabinet or container. Yes, > 37% Formalin we do store in this manner, but I have never heard of this > requirement for 10%NBF. I looked on line to many MSDS sheets from > different vendors and found only one that stated such storage > requirements for 10% NBF. During this search I found all but one > company states that formalin is not flammable. I brought this to the > Safety Officer. He agrees that it is not "flammable" but that it IS > "combustible". Combustible=Flash point of 100F to 200F. Of the dozen > sites I visited I found the following data concerning the Flash Point of > 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire > Protection Agency) guideline of no more than 1 gallon in a flammable > storage container and 1 gallon outside of a safety cabinet/container per > 100 square feet is already quite limiting. Using this guideline, we > have calculated acceptable volumes of the known flammables (Alcohols and > Xylenes) we can store. Adding 10% NBF to the equation will have us > traveling to our "bulk" storage area constantly. Does anyone out there > store 10%NBF in flammable cans/cabinets? > Riesen, Rebecca > Rebecca.Riesen@nchmd.org > NCH Healthcare Systems > Direct 239-436-5000 x2188 > Fax 239-436-6767 > > > Visit our website at http://www.nchmd.org > > > CONFIDENTIALITY NOTICE > > This email and any files transmitted with it are from the NCH Healthcare > System. > This message is confidential and is intended only for the addressee. If > you > are > not the intended recipient or have received this email in error, please > call > us > immediately at (239) 436-5000 and ask to speak to the message sender or > promptly > email the message sender of the delivery error and then delete the > message. > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DKBoyd <@t> chs.net Fri Sep 11 08:39:18 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Sep 11 08:35:57 2009 Subject: [Histonet] formalin storage In-Reply-To: Message-ID: No. We store only the 37% in a flammable cabinet. Ten percent formalin is not considered a flammable substance according to the MSDS for Richard Allen 10% formalin. Which brand are you using. Your storage should be according to the manufacturer's MSDS. Grant it, they should be all on the same page since it is a concrete formula. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "Riesen, Rebecca" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/11/2009 09:19 AM To cc Subject [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Tina.Hayes <@t> va.gov Fri Sep 11 08:37:46 2009 From: Tina.Hayes <@t> va.gov (Hayes, Tina J.) Date: Fri Sep 11 08:38:29 2009 Subject: [Histonet] formalin storage In-Reply-To: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> References: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> Message-ID: <01A34750423B874399B8AF6674D90A270454782E@VHAV01MSGA1.v01.med.va.gov> WE do not store our 10 NBF in flammable storage. However, Could you tell me where you found this direction of storing only 1 gallon per 100 sq. feet in a flammable cabinet, and 1 gallon outside? More specific than NFPA. I have been unable to find that anywhere, our safety folks tell us that if they are in a flammable cabinet the amount per room size doesn't matter. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Warren Sent: Friday, September 11, 2009 9:25 AM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Sep 11 08:39:21 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 11 08:39:48 2009 Subject: [Histonet] formalin storage In-Reply-To: Message-ID: To prevent getting cut on the knife. Emily Sours 09/11/2009 08:34 AM To "Jackie M O'Connor" , histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage I have to ask--what was the point of chain mail gloves?! Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Fri, Sep 11, 2009 at 9:31 AM, Jackie M O'Connor < Jackie.O'Connor@abbott.com> wrote: I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. "Jean Warren" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/11/2009 08:25 AM To "Riesen, Rebecca" , cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Fri Sep 11 08:43:39 2009 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Fri Sep 11 08:43:48 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: We store all our chemicals in a flammable storage cabinet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Friday, September 11, 2009 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From asmith <@t> mail.barry.edu Fri Sep 11 08:50:28 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 11 08:50:41 2009 Subject: [Histonet] formalin storage In-Reply-To: References: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> Message-ID: Does he require his wife to wear chain mail gloves while paring potatoes? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, September 11, 2009 9:31 AM To: Jean Warren Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Riesen, Rebecca Subject: Re: [Histonet] formalin storage I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. "Jean Warren" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/11/2009 08:25 AM To "Riesen, Rebecca" , cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Sep 11 09:00:36 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Sep 11 09:00:52 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <003d01ca32e4$2d4ffeb0$87effc10$@com> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> <003d01ca32e4$2d4ffeb0$87effc10$@com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> Cerner Millennium -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Friday, September 11, 2009 8:31 AM To: Mahoney,Janice A; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Janice, may I ask whom you have for your LIS? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 9:14 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From joseph-galbraith <@t> uiowa.edu Fri Sep 11 09:00:38 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 11 09:01:38 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: Amy: Exactly what do you mean by 'we store all our chemicals in a flammable storage cabinet'? By any chance, do you mean that you actually put everything into the same cabinet together? I would be very concerned about putting "everything" into one cabinet (flammable or not). Some materials should not be stored together (it's not safe and regulations require separate storage based on reactive properties). If this is the case, please consult your safety officer or use the MSDS sheet or the internet to determine proper storage. Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Friday, September 11, 2009 8:44 AM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] formalin storage We store all our chemicals in a flammable storage cabinet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Friday, September 11, 2009 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Sep 11 09:00:54 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Sep 11 09:01:42 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> Message-ID: <005c01ca32e8$5de15390$19a3fab0$@com> Janice, I have a few responses here, but let me give you one of them, for now. An interface cost roughly 10,000 per 'side'... so 20,000 without negotiation. What does Vantage cost? I'm not looking for your specific pricing, just ballpark pricing. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 9:14 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From LSebree <@t> uwhealth.org Fri Sep 11 09:05:35 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Sep 11 09:05:38 2009 Subject: [Histonet] Errors in the lab In-Reply-To: <50837.52197.qm@web65708.mail.ac4.yahoo.com> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A589035706B1@UWHC-MAIL01.uwhis.hosp.wisc.edu> We do it this way also. The person catching the error fills out the form (even if they caused the error!) and gives it to our supervisor. Then its up to his discretion what to do with the information. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Friday, September 11, 2009 8:26 AM To: histonet Subject: [Histonet] Errors in the lab I was just wondering how others are tracking mistakes.? We have a form that the employees fill out when they catch and rersolve mistakes.? I feel this gives me the opportunity to recognize problem areas (or people).? I was approached by one of my staff who feels that this may take away from a "team approach" and makes people feel picked on.? On one hand I can see that point, but the error forms are only seen by the person catching the error and me.? I guess I was wondering what everyone thinks? ? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristopher.Kalleberg <@t> unilever.com Fri Sep 11 09:07:45 2009 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Fri Sep 11 09:07:50 2009 Subject: [Histonet] automated immunostainer Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C06C159C0@NTRSEVS30002.s3.ms.unilever.com> All, I am looking for an automated immunostainer for research purposes. I would like something reliable, easy to use, and compatible with a wide variety of reagents and protocols. Does anyone have any recommendations? We are considering upgrading our lab from a manual lab to fully automated. If you could also give a ball park cost for the instruments it will be greatly appreciated. Thank you in adavance. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 (203) 381-5765 From joseph-galbraith <@t> uiowa.edu Fri Sep 11 09:07:31 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 11 09:08:34 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: I have also had 'general' safety inspectors (OSHA people who mostly focus on meat packing plants and food processors) make the same claim that we had to use metal or other cut resistant gloves. I had to demonstrate the task of cutting a frozen section to get them to understand. They still did not like it but they did recognize the problem. We do use chain mail gloves for the bone saw since it is a huge commercial band saw. Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, September 11, 2009 8:39 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin storage To prevent getting cut on the knife. Emily Sours 09/11/2009 08:34 AM To "Jackie M O'Connor" , histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage I have to ask--what was the point of chain mail gloves?! Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Fri, Sep 11, 2009 at 9:31 AM, Jackie M O'Connor < Jackie.O'Connor@abbott.com> wrote: I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. "Jean Warren" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/11/2009 08:25 AM To "Riesen, Rebecca" , cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rebecca.Riesen <@t> nchmd.org Fri Sep 11 09:09:28 2009 From: Rebecca.Riesen <@t> nchmd.org (Riesen, Rebecca) Date: Fri Sep 11 09:09:38 2009 Subject: [Histonet] formalin storage Message-ID: Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. From shive003 <@t> umn.edu Fri Sep 11 09:21:58 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 11 09:22:04 2009 Subject: [Histonet] AAVLD Histo sample block & slide retention policy Message-ID: <556B13AFEFCF4E6F87A234C8E2B31A93@auxs.umn.edu> For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL From tifei <@t> foxmail.com Fri Sep 11 09:23:04 2009 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Fri Sep 11 09:23:21 2009 Subject: [Histonet] formalin storage Message-ID: never store everything in one cabinet. As far as i know, HCl and formalin should not be stored together. If any of you know why... ------------------ Original ------------------ From: "Riesen, Rebecca"; Date: Fri, Sep 11, 2009 10:09 PM To: "histonet"; Subject: [Histonet] formalin storage Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri Sep 11 09:30:19 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Sep 11 09:30:25 2009 Subject: [Histonet] Re: Permount question In-Reply-To: <361429220909101352w748222cs10e4e5f27796440@mail.gmail.com> References: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> <361429220909101352w748222cs10e4e5f27796440@mail.gmail.com> Message-ID: Just so a stereotype doesn't get started here...I was 1st taught in the (straight) use of Permount by such an Asian technician (who couldn't speak English, but was such a wonderful teacher I learned more from watching her than anything!) :-) --On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen wrote: > Thanks for all the replies, I guess these weird Asian technicians who are > highly looked up to taught me something wrong and it's been propagated in > the department. Thanks again! > > On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen > wrote: > >> Hello, >> Did anyone ever use Permount straight without cutting it with some >> solvent? Journal articles mention using Permount to coverslip, and the >> post-doc I work with thinks that it isn't diluted. >> >> I think maybe people just universally "know" that it should be diluted so >> it isn't mentioned in the Materials and Methods. Any thoughts? >> >> Leslie >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From leiker <@t> buffalo.edu Fri Sep 11 09:39:55 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Sep 11 09:40:02 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: <22184B7FA3050C8D27B2015E@CDYwxp1931.ad.med.buffalo.edu> Flash pt of 100-200 F? I just converted it - that's around 37-93 C. That doesn't sound right. We routinely make our own 10% NBF (4% PFA) and heat it at 60-80 C in the process. We don't have to label the waste as combustible. Merced --On Friday, September 11, 2009 9:15 AM -0400 "Riesen, Rebecca" wrote: > > > We have been directed by our Safety Officer to store all formalin (37% > and 10% NBF) in a flammable storage room, cabinet or container. Yes, > 37% Formalin we do store in this manner, but I have never heard of this > requirement for 10%NBF. I looked on line to many MSDS sheets from > different vendors and found only one that stated such storage > requirements for 10% NBF. During this search I found all but one > company states that formalin is not flammable. I brought this to the > Safety Officer. He agrees that it is not "flammable" but that it IS > "combustible". Combustible=Flash point of 100F to 200F. Of the dozen > sites I visited I found the following data concerning the Flash Point of > 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire > Protection Agency) guideline of no more than 1 gallon in a flammable > storage container and 1 gallon outside of a safety cabinet/container per > 100 square feet is already quite limiting. Using this guideline, we > have calculated acceptable volumes of the known flammables (Alcohols and > Xylenes) we can store. Adding 10% NBF to the equation will have us > traveling to our "bulk" storage area constantly. Does anyone out there > store 10%NBF in flammable cans/cabinets? > Riesen, Rebecca > Rebecca.Riesen@nchmd.org > NCH Healthcare Systems > Direct 239-436-5000 x2188 > Fax 239-436-6767 > > > Visit our website at http://www.nchmd.org > > > CONFIDENTIALITY NOTICE > > This email and any files transmitted with it are from the NCH Healthcare > System. This message is confidential and is intended only for the > addressee. If you are not the intended recipient or have received this > email in error, please call us immediately at (239) 436-5000 and ask to > speak to the message sender or promptly email the message sender of the > delivery error and then delete the message. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Erin.Martin <@t> ucsf.edu Fri Sep 11 09:41:51 2009 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Fri Sep 11 09:41:56 2009 Subject: [Histonet] Formalin storage Message-ID: <379A927A452F3D43A3C8705F4E67905F0CE82711B5@EX05.net.ucsf.edu> We too were told by our internal safety compliance officer that 10% NBF has to be stored in flammable cabinets and that is now how we store it. We were also told that our general purpose household bleach has to be in a flammable cabinet.... Erin Martin UCSF Dermatopathology From leiker <@t> buffalo.edu Fri Sep 11 09:43:05 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Sep 11 09:43:10 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: <46F6196F7985F576EFFE2FD2@CDYwxp1931.ad.med.buffalo.edu> that makes sense now...when we make the stuff, we ALWAYS do it in a fume hood... --On Friday, September 11, 2009 10:09 AM -0400 "Riesen, Rebecca" wrote: > > Thank you all! I have received many responses already concerning 10% > Neutral Buffered Formalin (NBF) storage. Only one person has stated > that they store all chemicals, including formalin, in safety cabinets. > I just want to clarify something. All MSDS's I studied stated that 10% > NBF indeed is NOT flammable, but the Flash Point is under 200 F on all > but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 > rules on flammables storage include all Class I, II and IIIA liquids. > Class IIIA liquids include those with a Flashpoint of less than 200 F. > That would include 10% NBF. It appears to be the formaldehyde fume > (which we all know very well) is the combustible portion no matter if it > is 96.7% water. Most manufacturer's recommendations are for storage in a > tightly sealed container, probably to keep those nasty fumes inside. > One would think that would be sufficient. > Riesen, Rebecca > Rebecca.Riesen@nchmd.org > NCH Healthcare Systems > Direct 239-436-5000 x2188 > Fax 239-436-6767 > > > Visit our website at http://www.nchmd.org > > > CONFIDENTIALITY NOTICE > > This email and any files transmitted with it are from the NCH Healthcare > System. This message is confidential and is intended only for the > addressee. If you are not the intended recipient or have received this > email in error, please call us immediately at (239) 436-5000 and ask to > speak to the message sender or promptly email the message sender of the > delivery error and then delete the message. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From mike <@t> pathview.com Fri Sep 11 09:57:51 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Sep 11 09:58:27 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> <003d01ca32e4$2d4ffeb0$87effc10$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> Message-ID: <007f01ca32f0$4bc34a30$e349de90$@com> Jan, we'll be at APIII in 10 days so it's going to be hard to turn around and be at NSH, but we're trying. I'd be very happy to get your and other people's opinion of our system. We think we have a very straight forward and comprehensive approach to workflow processing. It's really an unfair, but very simple advantage that we have. We built our system from the ground up with workflow in mind. Other vendors built their systems years ago and over the years they've more or less just added on. I don't know if you've ever been in the Northeast, but have you ever seen those farmhouses that are a hundred or two years old. You can see where each occupant just added on to the house. It's a big difference when you can initially build the house to provide the needed amount of living space. Our software looks like that compared to other vendors. Let me fully respond to your Vantage comment. (and btw, if anyone knows a ballpark for Vantage, please email me privately.) I'll start out with one of those SHOUTED statements. I HATE Vantage and products of that ilk. Now, let me explain. I love WHAT Vantage does. I just really, really think it all belongs in the LIS. All this tracking and audit information needs to be in ONE system. Having two systems means two purchasing costs, two systems to maintain, two systems to look into while you're trying to track down a problem, and on and on and on. The reason Ventana and Dako and others can sell this product is because LIS vendors have been slow to adopt these changes themselves. Not to toot our own horn too loud, but all this data is contained in our system. In fact, all this data is in ONE query screen not multiple screens. That's another thing that drives me nuts -- 10,000 tabs or screens to see data. Show me the things that I am most likely interested in in a query screen and make other things like audit or tracking information a quick click away. Most of the times when I query for results I need to see results. Show those results to me and only those results. However, when I need something more, don't make me go to some other tab or menu or something else that I need to use multiple clicks or tabs or whatever the heck it is. One click to see tracking information, period. I went on that 'rant' to prove a point. With Vantage, you can't do this in one simple click because it's a separate system. Now, is it better than nothing? Sure, it is. If I was you and I was stuck on my existing LIS, I'd probably go for Vantage because it's cheaper than a new system, but to be honest, I?m not even sure of that. I know Vantage is expensive. I just don't know 'how' expensive. Finally, don't get me wrong. I?m not mad or upset with Ventana or Dako or whomever. They're doing a good job filling a niche. If I'm upset with anyone, it's the LIS vendors. Of course, they're my competitors, so in a way I'm happy. Thank you all for listening. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 10:01 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Cerner Millennium -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Friday, September 11, 2009 8:31 AM To: Mahoney,Janice A; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Janice, may I ask whom you have for your LIS? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 9:14 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From JEllin <@t> yumaregional.org Fri Sep 11 10:07:33 2009 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Sep 11 10:09:39 2009 Subject: [Histonet] Ventana ULTRA Immuno Stainer References: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8016113D8@EXCHANGECLUSTER.yumaregional.local> Excellent stainer, but you need to look at your process to help facilitate the technology. if you have nay questions you can reach me. Jesus Ellin Yuma Regional Medical Center 928-336-1144 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gomez, Milton Sent: Thu 9/10/2009 7:35 PM To: Histonet Subject: [Histonet] Ventana ULTRA Immuno Stainer Dear Histonetters, Is anyone out there using the Ventana ULTRA Immuno Stainer? What is your experience with them? What are the advantages, disadvantages, pros and cons? Thank you very much in advance? Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.gomez@aruplab.com Web: www.aruplab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From akemiat3377 <@t> yahoo.com Fri Sep 11 10:20:43 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Sep 11 10:20:46 2009 Subject: [Histonet] antibody validation Message-ID: <332045.51983.qm@web31304.mail.mud.yahoo.com> Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation.? I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC R&D for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab.? I would like your assistance and input on how you are validating new antibodies. ? Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases.? Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases).? Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation.? I am curious how you approach validation.? Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com From Lynn.Burton <@t> Illinois.gov Fri Sep 11 10:35:57 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Sep 11 10:38:18 2009 Subject: [Histonet] AAVLD Histo sample block & slide retention policy References: <556B13AFEFCF4E6F87A234C8E2B31A93@auxs.umn.edu> Message-ID: I read our SOP's on storage and referred to Dr. webb, our pathologist. His feeling was that they, AAVLD, leave that up to each lab depending on the state statutes regarding law suits. Our SOP says seven years on blocks and nine years on slides. I would agree with you that as long as you have blocks, the slides would be less important. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/11/2009 9:21 AM To: histonet Subject: [Histonet] AAVLD Histo sample block & slide retention policy For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> smdc.org Fri Sep 11 10:39:18 2009 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Fri Sep 11 10:39:35 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <007f01ca32f0$4bc34a30$e349de90$@com> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> <003d01ca32e4$2d4ffeb0$87effc10$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> <007f01ca32f0$4bc34a30$e349de90$@com> Message-ID: Hi Michael, I have been reading with great curiosity your postings on Histonet. Other vendors have been shot in the knees for doing what you appear to be doing! You are openly soliciting! I personally don't care to read your opinions about the various LIS and LEAN based systems that are available - only because I view you as a direct competitor! Your system may be as great you tell us - but I see it as a sales pitch. If you want to sell to histologists - you had best be at NSH. That would allow histologists to view your product right next to your competitors. I may be wrong, and if I am please correct me. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, September 11, 2009 9:58 AM To: 'Mahoney,Janice A'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Jan, we'll be at APIII in 10 days so it's going to be hard to turn around and be at NSH, but we're trying. I'd be very happy to get your and other people's opinion of our system. We think we have a very straight forward and comprehensive approach to workflow processing. It's really an unfair, but very simple advantage that we have. We built our system from the ground up with workflow in mind. Other vendors built their systems years ago and over the years they've more or less just added on. I don't know if you've ever been in the Northeast, but have you ever seen those farmhouses that are a hundred or two years old. You can see where each occupant just added on to the house. It's a big difference when you can initially build the house to provide the needed amount of living space. Our software looks like that compared to other vendors. Let me fully respond to your Vantage comment. (and btw, if anyone knows a ballpark for Vantage, please email me privately.) I'll start out with one of those SHOUTED statements. I HATE Vantage and products of that ilk. Now, let me explain. I love WHAT Vantage does. I just really, really think it all belongs in the LIS. All this tracking and audit information needs to be in ONE system. Having two systems means two purchasing costs, two systems to maintain, two systems to look into while you're trying to track down a problem, and on and on and on. The reason Ventana and Dako and others can sell this product is because LIS vendors have been slow to adopt these changes themselves. Not to toot our own horn too loud, but all this data is contained in our system. In fact, all this data is in ONE query screen not multiple screens. That's another thing that drives me nuts -- 10,000 tabs or screens to see data. Show me the things that I am most likely interested in in a query screen and make other things like audit or tracking information a quick click away. Most of the times when I query for results I need to see results. Show those results to me and only those results. However, when I need something more, don't make me go to some other tab or menu or something else that I need to use multiple clicks or tabs or whatever the heck it is. One click to see tracking information, period. I went on that 'rant' to prove a point. With Vantage, you can't do this in one simple click because it's a separate system. Now, is it better than nothing? Sure, it is. If I was you and I was stuck on my existing LIS, I'd probably go for Vantage because it's cheaper than a new system, but to be honest, I'm not even sure of that. I know Vantage is expensive. I just don't know 'how' expensive. Finally, don't get me wrong. I'm not mad or upset with Ventana or Dako or whomever. They're doing a good job filling a niche. If I'm upset with anyone, it's the LIS vendors. Of course, they're my competitors, so in a way I'm happy. Thank you all for listening. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 10:01 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Cerner Millennium -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Friday, September 11, 2009 8:31 AM To: Mahoney,Janice A; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Janice, may I ask whom you have for your LIS? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 9:14 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From rjbuesa <@t> yahoo.com Fri Sep 11 10:40:49 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 11 10:41:03 2009 Subject: [Histonet] Permount question In-Reply-To: <361429220909101303p22132d12q256e6a97f931baed@mail.gmail.com> Message-ID: <796574.11922.qm@web65703.mail.ac4.yahoo.com> I always diluted Permount to give it the fluidity I desired for manual coverslipping and faster drying. Ren? J. --- On Thu, 9/10/09, Leslie Chen wrote: From: Leslie Chen Subject: [Histonet] Permount question To: histonet@lists.utsouthwestern.edu Date: Thursday, September 10, 2009, 4:03 PM Hello, Did anyone ever use Permount straight without cutting it with some solvent? Journal articles mention using Permount to coverslip, and the post-doc I work with thinks that it isn't diluted. I think maybe people just universally "know" that it should be diluted so it isn't mentioned in the Materials and Methods.? Any thoughts? Leslie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 11 10:46:42 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 11 10:47:01 2009 Subject: [Histonet] automated immunostainer In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C06C159C0@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <159441.13740.qm@web65715.mail.ac4.yahoo.com> I always used the DAKO autostainer but lately there have been some costs issues raised in HistoNet as well as many very good opinions about the BondMax from Leica. You could check them both or ask for a demo. Ren? J. --- On Fri, 9/11/09, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] automated immunostainer To: histonet@lists.utsouthwestern.edu Date: Friday, September 11, 2009, 10:07 AM All, I am looking for an automated immunostainer for research purposes.? I would like something reliable, easy to use, and compatible with a wide variety of reagents and protocols. Does anyone have any recommendations? We are considering upgrading our lab from a manual lab to fully automated.? If you could also give a ball park cost for the instruments it will be greatly appreciated.? Thank you in adavance. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 (203) 381-5765 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Sep 11 10:50:44 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Sep 11 10:50:56 2009 Subject: [Histonet] antibody validation In-Reply-To: <332045.51983.qm@web31304.mail.mud.yahoo.com> References: <332045.51983.qm@web31304.mail.mud.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4CEBB28B52@EXMBMCB15.ucsfmedicalcenter.org> Akemi, We follow pretty much what you outline below. I believe following strict and comprehensive validation will save you headaches later on. You want to 1) convince yourself that the antibody works as advertised and as expected according to the literature and 2)Identify and solve any technical issues that may cause spurious results. You can't do that with a one-off test of one tissue sample. After a well-done validation you should have great confidence that it is being done right and works correctly. It is very useful to have the validation data in hand if anyone questions your test results. Suggested references: Recommendations for Improved Standardization of Immunohistochemistry, Goldstein, NS, et.al., and members of Ad-Hoc Committee on Immunohistochemical Standardization, Appl Immunohistochem Mol Morph, 2007 15(2): 124-133 A Practical Approach for Evaluating New Antibodies in the Clinical Immunohistochemistry Laboratory, Hsi, ED, Arc Pathol Lab Med. 2001; 125: 289-294 Quality Assurance For Immuncytochemistry: Approved Guideline, Clinical Laboratory Standards Institute (formerly NCCLS), Wayne PA, USA, publication MM4-A, Vol. 19, No. 26, 1999. www.clsi.org Book chapters on IHC Validation and QC: Quality Management in Immunohistochemistry, Brown RW, in Quality Management in Anatomic Pathology, Nakhleh RE, and Fitzgibbons PL, Eds. College of American Pathologists, Northfield, IL, USA, 2005. www.cap.org. Theoretical and Practical Aspects of Test Performance, in Immunohistology: A Diagnostic Tool for the Surgical Pathologist. 3rd. Ed., Volume 19 in Major Problems in Pathology, Taylor CR and Cote RJ, Eds., W.B Saunders, Philadelphia, 2005 Immunohistochemistry Quality Control, Hladik, CL and White, CL, in Theory and Practice of Histological Techniques, 4th Ed., Bancroft JD and Gamble M, Eds., Churchill Livingstone, 2007 Techniques of Immunohistochemistry: Principles, Pitfalls and Standardization, Taylor CR, et.al., in Diagnostic Immunohistochemistry, Dabbs, DJ, Ed., 2nd Edition, Churchill Livingstone, Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, September 11, 2009 8:21 AM To: histonet Subject: [Histonet] antibody validation Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation.? I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC R&D for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab.? I would like your assistance and input on how you are validating new antibodies. ? Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases.? Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases).? Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation.? I am curious how you approach validation.? Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Sep 11 10:56:37 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Sep 11 10:56:45 2009 Subject: [Histonet] Vendor Gripe (was: Slide labels used with IHC Instruments) In-Reply-To: References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> <003d01ca32e4$2d4ffeb0$87effc10$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> <007f01ca32f0$4bc34a30$e349de90$@com> Message-ID: <4AAA7335.8090201@umdnj.edu> > Other vendors have been shot in the knees for doing what you appear to be doing! You are openly soliciting! Since we're on the subject, I also wish vendors would stop searching the Histonet Archives for certain keywords involving their products, then cold-calling/emailing/spamming people who posted/replied to said threads with sales-pitches. For example, not to name names (Renee Schultz at Thermo-Fisher) but someone recently wrote me a series of sales-soliciting emails, explaining that they were "following up on an earlier conversation" we had had. Since I tend to remember actual conversations I've had (and her name/inquiry didn't sound familiar), I questioned her, and she freely admitted to perusing the archives and actually maintaining a sort of database of our comments on this list to use for future sales pitches... one of which I received... 6 months after the fact... despite the fact that I was answering someone else's question about a product, not complaining about it or searching for a new vendor myself! From NMargaryan <@t> childrensmemorial.org Fri Sep 11 11:10:17 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 11 11:11:32 2009 Subject: [Histonet] type of paraffin and polymer Message-ID: Dear Histonetters, I quite need answer as soon as it possible, PLEASE! I am working with mouse tissue and tumors. What type of paraffin is the best for processing and embedding to cut 4? nice sections? Thanks in advance, Naira From rjbuesa <@t> yahoo.com Fri Sep 11 11:17:02 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 11 11:17:10 2009 Subject: [Histonet] type of paraffin and polymer In-Reply-To: Message-ID: <807358.17625.qm@web65702.mail.ac4.yahoo.com> Paraplast Ren? J. --- On Fri, 9/11/09, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] type of paraffin and polymer To: "histonet@lists.utsouthwestern.edu" Date: Friday, September 11, 2009, 12:10 PM Dear Histonetters, I quite need answer as soon as it possible, PLEASE! I am working with mouse tissue and tumors. What type of paraffin is the best for processing and embedding to cut 4? nice sections? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Sep 11 11:19:57 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Sep 11 11:20:01 2009 Subject: [Histonet] type of paraffin and polymer In-Reply-To: References: Message-ID: <005301ca32fb$b42cb790$1c8626b0$@net> Paraffin Prills from Polyscientific R&D is great for animal and human work. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, September 11, 2009 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] type of paraffin and polymer Dear Histonetters, I quite need answer as soon as it possible, PLEASE! I am working with mouse tissue and tumors. What type of paraffin is the best for processing and embedding to cut 4? nice sections? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Sep 11 12:17:56 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Sep 11 12:18:43 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> <003d01ca32e4$2d4ffeb0$87effc10$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> <007f01ca32f0$4bc34a30$e349de90$@com> Message-ID: <003c01ca3303$e261f730$a725e590$@com> Sheila, I apologize to you and Peter and to whomever else I have offended. I view Histonet as a place where people can go for knowledge and where people can share their experiences. I was trying to share my experience with LIS systems and information system technology in general. I do admit that I had one paragraph in there about our own product, but I was trying to illustrate a point. I thought I had spent a bit of time talking about Ventana and Dako and as you've read my other posts, you've seen me mention Leica and others as well. I always talk about things from an information technology perspective. That's what I do and that's what I believe I am good at. I don't know if you're from Ventana or Daka or whomever, but please notice that I stated I have no problems with what you guys are doing. In fact, I applaud you for filling a very needed role in the industry. In other posts I've perhaps compared Leica and Ventana, but I've only done so from an information technology/interface/labeling perspective. I know very little about the chemistry involved and therefore I am not qualified to say which instrument is better suited for the task. I think everyone should ask more of their LIS vendors. In that way everyone benefits. But, how do people know what to ask for if they don't know that certain things are possible. I was hoping to encourage people to ask for further functionality from their LIS vendors... and yes, I fully admit that there was some component of publicity. However, I do feel like it was 90/10 or 80/20 knowledge vs publicity. So, please forgive me I offended anyone, but I still think there are a lot of information technology aspects that people are not aware of and that a majority of people would like to learn about. Everyone will notice that I always publish all of my contact information and my company name. I'm not trying to 'sneak something' by anyone. Competition is good for everyone. Is it not? And finally, Sheila, I wouldn't call you 'wrong'. You've got an opinion and I respect it. I just disagree. I'll shut up now, and if you or anyone else would like to talk to me please feel free to do so. (My contact information is below, but to make my email address more obvious, it is mike@pathview.com) P.S. We're at APIII at the end of September. NSH is roughly 2 weeks after that. It's hard for us to be at both conferences and APIII is more computer oriented so we thought it would be a better fit for us. If you happen to be there, please come by. You don't even have to mention your name. I'd love to hear some comments on our system. The more we hear, the better our system will become. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Tapper, Sheila J. [mailto:STapper@smdc.org] Sent: Friday, September 11, 2009 11:39 AM To: Michael Mihalik; Mahoney,Janice A; Cordova, Jean; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Hi Michael, I have been reading with great curiosity your postings on Histonet. Other vendors have been shot in the knees for doing what you appear to be doing! You are openly soliciting! I personally don't care to read your opinions about the various LIS and LEAN based systems that are available - only because I view you as a direct competitor! Your system may be as great you tell us - but I see it as a sales pitch. If you want to sell to histologists - you had best be at NSH. That would allow histologists to view your product right next to your competitors. I may be wrong, and if I am please correct me. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, September 11, 2009 9:58 AM To: 'Mahoney,Janice A'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Jan, we'll be at APIII in 10 days so it's going to be hard to turn around and be at NSH, but we're trying. I'd be very happy to get your and other people's opinion of our system. We think we have a very straight forward and comprehensive approach to workflow processing. It's really an unfair, but very simple advantage that we have. We built our system from the ground up with workflow in mind. Other vendors built their systems years ago and over the years they've more or less just added on. I don't know if you've ever been in the Northeast, but have you ever seen those farmhouses that are a hundred or two years old. You can see where each occupant just added on to the house. It's a big difference when you can initially build the house to provide the needed amount of living space. Our software looks like that compared to other vendors. Let me fully respond to your Vantage comment. (and btw, if anyone knows a ballpark for Vantage, please email me privately.) I'll start out with one of those SHOUTED statements. I HATE Vantage and products of that ilk. Now, let me explain. I love WHAT Vantage does. I just really, really think it all belongs in the LIS. All this tracking and audit information needs to be in ONE system. Having two systems means two purchasing costs, two systems to maintain, two systems to look into while you're trying to track down a problem, and on and on and on. The reason Ventana and Dako and others can sell this product is because LIS vendors have been slow to adopt these changes themselves. Not to toot our own horn too loud, but all this data is contained in our system. In fact, all this data is in ONE query screen not multiple screens. That's another thing that drives me nuts -- 10,000 tabs or screens to see data. Show me the things that I am most likely interested in in a query screen and make other things like audit or tracking information a quick click away. Most of the times when I query for results I need to see results. Show those results to me and only those results. However, when I need something more, don't make me go to some other tab or menu or something else that I need to use multiple clicks or tabs or whatever the heck it is. One click to see tracking information, period. I went on that 'rant' to prove a point. With Vantage, you can't do this in one simple click because it's a separate system. Now, is it better than nothing? Sure, it is. If I was you and I was stuck on my existing LIS, I'd probably go for Vantage because it's cheaper than a new system, but to be honest, I'm not even sure of that. I know Vantage is expensive. I just don't know 'how' expensive. Finally, don't get me wrong. I'm not mad or upset with Ventana or Dako or whomever. They're doing a good job filling a niche. If I'm upset with anyone, it's the LIS vendors. Of course, they're my competitors, so in a way I'm happy. Thank you all for listening. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 10:01 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Cerner Millennium -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Friday, September 11, 2009 8:31 AM To: Mahoney,Janice A; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Janice, may I ask whom you have for your LIS? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, September 11, 2009 9:14 AM To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA, workload stats, etc, etc. It allows for the best process flow you can imagine. I'm very into "processes" and this system will reduce if not eliminate your errors. We were already a LEAN lab and with the addition of Vantage we reduced TAT by about 30% and virtually eliminated labeling errors. I'd suggest looking into Vantage before I'd venture into more costly interfaces that will just get you a label that can be read by an instrument. Vantage will give you so much more. Check it out. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Thursday, September 10, 2009 4:40 PM To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labels used with IHC Instruments Actually, Jean it's a different story for Leica. With Ventana you HAVE to use THEIR labels. That's not the case with Leica. Succinctly, this means that you can use your LIS labels on a slide and the Lecia will read them and that's that. On the Ventana equipment, the slide MUST have the VENTANA slide label on the slide. Some powerpath users have gotten the powerpath barcode on the Ventana slide label and we're about to try and place our LIS slide label on the back of the slide. Purely, and I mean PURELY, from a labeling process, I would argue that the Leica is better suited for process flow. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, Jean Sent: Thursday, September 10, 2009 5:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labels used with IHC Instruments While our lab doesn't have the Ventana stainer I am assuming that the principle is the same for the Leica Bond. We are using slide labels generated from PowerPath on our slides for the Bond Immunostainer.without any issues. We do have an interface in place. Jean Cordova HT/HTL (ASCP) Pathology Manager Northwest Pathology Services LLC 541-341-8039 This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From carrolpb <@t> umdnj.edu Fri Sep 11 12:41:31 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Sep 11 12:41:44 2009 Subject: [Histonet] Slide labels used with IHC Instruments In-Reply-To: <003c01ca3303$e261f730$a725e590$@com> References: <18E3095CC1A30E4684F55D6D757C8D2E01A6D10E@phoex1.peacehealth.org> <035101ca325f$54fe3bf0$fefab3d0$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE89@EXCHMBC2.ad.ah.local> <003d01ca32e4$2d4ffeb0$87effc10$@com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE8C@EXCHMBC2.ad.ah.local> <007f01ca32f0$4bc34a30$e349de90$@com> <003c01ca3303$e261f730$a725e590$@com> Message-ID: <4AAA8BCB.8080504@umdnj.edu> > yes, I fully admit that there was some component of publicity. However, I do feel like it was 90/10 or 80/20 knowledge vs publicity. I guess that's the problem. As a non-commercial list, the ratio should be more like 100/0. > I don't know if you're from Ventana or Daka or whomever I'm "from" neither and actually use no products by either of those vendors, nor yours. I joined Histonet to give/get histology advice on protocols and laboratory procedure, not to constantly be barraged- both on and off list- by vendors trying to push their products, no matter how subtly. Although they may not violate any specific "letter of the law" on Histonet, commercial posts or tie-ins, no matter how tasteful or friendly, do definitely violate the "spirit" of the list, at least in my eyes. Just my two cents... Michael Mihalik wrote: > Sheila, I apologize to you and Peter and to whomever else I have offended. > > I view Histonet as a place where people can go for knowledge and where > people can share their experiences. > > I was trying to share my experience with LIS systems and information system > technology in general. I do admit that I had one paragraph in there about > our own product, but I was trying to illustrate a point. I thought I had > spent a bit of time talking about Ventana and Dako and as you've read my > other posts, you've seen me mention Leica and others as well. I always talk > about things from an information technology perspective. That's what I do > and that's what I believe I am good at. I don't know if you're from Ventana > or Daka or whomever, but please notice that I stated I have no problems with > what you guys are doing. In fact, I applaud you for filling a very needed > role in the industry. In other posts I've perhaps compared Leica and > Ventana, but I've only done so from an information > technology/interface/labeling perspective. I know very little about the > chemistry involved and therefore I am not qualified to say which instrument > is better suited for the task. > > I think everyone should ask more of their LIS vendors. In that way everyone > benefits. But, how do people know what to ask for if they don't know that > certain things are possible. I was hoping to encourage people to ask for > further functionality from their LIS vendors... and yes, I fully admit that > there was some component of publicity. However, I do feel like it was 90/10 > or 80/20 knowledge vs publicity. > > So, please forgive me I offended anyone, but I still think there are a lot > of information technology aspects that people are not aware of and that a > majority of people would like to learn about. Everyone will notice that I > always publish all of my contact information and my company name. I'm not > trying to 'sneak something' by anyone. Competition is good for everyone. > Is it not? > > > And finally, Sheila, I wouldn't call you 'wrong'. You've got an opinion and > I respect it. I just disagree. I'll shut up now, and if you or anyone else > would like to talk to me please feel free to do so. (My contact information > is below, but to make my email address more obvious, it is > mike@pathview.com) > > > P.S. We're at APIII at the end of September. NSH is roughly 2 weeks after > that. It's hard for us to be at both conferences and APIII is more computer > oriented so we thought it would be a better fit for us. If you happen to be > there, please come by. You don't even have to mention your name. I'd love > to hear some comments on our system. The more we hear, the better our > system will become. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > > -----Original Message----- > From: Tapper, Sheila J. [mailto:STapper@smdc.org] > Sent: Friday, September 11, 2009 11:39 AM > To: Michael Mihalik; Mahoney,Janice A; Cordova, Jean; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide labels used with IHC Instruments > > Hi Michael, > > I have been reading with great curiosity your postings on Histonet. Other > vendors have been shot in the knees for doing what you appear to be doing! > You are openly soliciting! > > I personally don't care to read your opinions about the various LIS and LEAN > based systems that are available - only because I view you as a direct > competitor! Your system may be as great you tell us - but I see it as a > sales pitch. > > If you want to sell to histologists - you had best be at NSH. That would > allow histologists to view your product right next to your competitors. > > I may be wrong, and if I am please correct me. > > Sheila > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Mihalik > Sent: Friday, September 11, 2009 9:58 AM > To: 'Mahoney,Janice A'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide labels used with IHC Instruments > > Jan, we'll be at APIII in 10 days so it's going to be hard to turn around > and be at NSH, but we're trying. I'd be very happy to get your and other > people's opinion of our system. We think we have a very straight forward > and comprehensive approach to workflow processing. It's really an unfair, > but very simple advantage that we have. We built our system from the ground > up with workflow in mind. Other vendors built their systems years ago and > over the years they've more or less just added on. I don't know if you've > ever been in the Northeast, but have you ever seen those farmhouses that are > a hundred or two years old. You can see where each occupant just added on > to the house. It's a big difference when you can initially build the house > to provide the needed amount of living space. Our software looks like that > compared to other vendors. > > Let me fully respond to your Vantage comment. (and btw, if anyone knows a > ballpark for Vantage, please email me privately.) > > I'll start out with one of those SHOUTED statements. > > I HATE Vantage and products of that ilk. Now, let me explain. > > I love WHAT Vantage does. I just really, really think it all belongs in the > LIS. All this tracking and audit information needs to be in ONE system. > Having two systems means two purchasing costs, two systems to maintain, two > systems to look into while you're trying to track down a problem, and on and > on and on. > > The reason Ventana and Dako and others can sell this product is because LIS > vendors have been slow to adopt these changes themselves. Not to toot our > own horn too loud, but all this data is contained in our system. In fact, > all this data is in ONE query screen not multiple screens. That's another > thing that drives me nuts -- 10,000 tabs or screens to see data. Show me > the things that I am most likely interested in in a query screen and make > other things like audit or tracking information a quick click away. Most of > the times when I query for results I need to see results. Show those > results to me and only those results. However, when I need something more, > don't make me go to some other tab or menu or something else that I need to > use multiple clicks or tabs or whatever the heck it is. One click to see > tracking information, period. > > I went on that 'rant' to prove a point. With Vantage, you can't do this in > one simple click because it's a separate system. > > Now, is it better than nothing? Sure, it is. If I was you and I was stuck > on my existing LIS, I'd probably go for Vantage because it's cheaper than a > new system, but to be honest, I'm not even sure of that. I know Vantage is > expensive. I just don't know 'how' expensive. > > Finally, don't get me wrong. I'm not mad or upset with Ventana or Dako or > whomever. They're doing a good job filling a niche. If I'm upset with > anyone, it's the LIS vendors. Of course, they're my competitors, so in a > way I'm happy. > > Thank you all for listening. > > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > > -----Original Message----- > From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] > Sent: Friday, September 11, 2009 10:01 AM > To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide labels used with IHC Instruments > > Cerner Millennium > > > -----Original Message----- > From: Michael Mihalik [mailto:mike@pathview.com] > Sent: Friday, September 11, 2009 8:31 AM > To: Mahoney,Janice A; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide labels used with IHC Instruments > > Janice, may I ask whom you have for your LIS? > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > > -----Original Message----- > From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] > Sent: Friday, September 11, 2009 9:14 AM > To: 'Michael Mihalik'; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide labels used with IHC Instruments > > > We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, > QA, workload stats, etc, etc. It allows for the best process flow you can > imagine. I'm very into "processes" and this system will reduce if not > eliminate your errors. We were already a LEAN lab and with the addition of > Vantage we reduced TAT by about 30% and virtually eliminated labeling > errors. > I'd suggest looking into Vantage before I'd venture into more costly > interfaces that will just get you a label that can be read by an instrument. > Vantage will give you so much more. Check it out. > Jan Mahoney > Omaha, NE > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Mihalik > Sent: Thursday, September 10, 2009 4:40 PM > To: 'Cordova, Jean'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide labels used with IHC Instruments > > Actually, Jean it's a different story for Leica. > > With Ventana you HAVE to use THEIR labels. That's not the case with Leica. > Succinctly, this means that you can use your LIS labels on a slide and the > Lecia will read them and that's that. > > On the Ventana equipment, the slide MUST have the VENTANA slide label on the > slide. Some powerpath users have gotten the powerpath barcode on the > Ventana slide label and we're about to try and place our LIS slide label on > the back of the slide. > > > Purely, and I mean PURELY, from a labeling process, I would argue that the > Leica is better suited for process flow. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cordova, > Jean > Sent: Thursday, September 10, 2009 5:17 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide labels used with IHC Instruments > > While our lab doesn't have the Ventana stainer I am assuming that the > principle is the same for the Leica Bond. We are using slide labels > generated from PowerPath on our slides for the Bond > Immunostainer.without any issues. We do have an interface in place. > > > > Jean Cordova HT/HTL (ASCP) > > Pathology Manager > > Northwest Pathology Services LLC > > 541-341-8039 > > > > > > This message is intended solely for the use of the individual and entity to > whom it is addressed, and may contain information that is privileged, > confidential, and exempt from disclosure under applicable state and federal > laws. If you are not the addressee, or are not authorized to receive for > the intended addressee, you are hereby notified that you may not use, copy, > distribute, or disclose to anyone this message or the information contained > herein. If you have received this message in error, immediately advise the > sender by reply email and destroy this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, > Alegent Health is faithful to the healing ministry of Jesus Christ, > providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly prohibited > and may be unlawful. If you received this communication in error, please > inform us of the erroneous delivery by return e-mail message from your > computer. Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for the > presence of viruses before opening. Alegent Health accepts no liability for > any damage caused by any virus transmitted by this e-mail. Thank you for > your cooperation. > > > > > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, > Alegent Health is faithful to the healing ministry of Jesus Christ, > providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly prohibited > and may be unlawful. If you received this communication in error, please > inform us of the erroneous delivery by return e-mail message from your > computer. Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for the > presence of viruses before opening. Alegent Health accepts no liability for > any damage caused by any virus transmitted by this e-mail. Thank you for > your cooperation. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain confidential and > privileged information for the use of the designated recipients named above. > If you are not the intended recipient, you are hereby notified that you have > received this communication in error and that any review, disclosure, > dissemination, distribution or copying of it or its contents is prohibited. > As required by federal and state laws, you need to hold this information as > privileged and confidential. If you have received this communication in > error, please notify the sender and destroy all copies of this communication > and any attachments. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mark.rand <@t> amgmicro.com Fri Sep 11 12:49:52 2009 From: mark.rand <@t> amgmicro.com (Mark Rand) Date: Fri Sep 11 12:50:00 2009 Subject: [Histonet] Re: mercury vs led lights **Commercial Response** Message-ID: Full disclosure: My company manufactures an LED fluorescence microscope :-) Merced's information from the Zeiss rep is partially correct and needs some clarification. There is a "gap" in the spectrum of available LED's from 530 to 590 nm that would be powerful enough for fluorescence excitation. However, there aren't many fluorophores in that range that can't be covered by either 530 or 590 nm excitation. In fact, the examples cited (Alexa Fluor 555, Cy3, and TRITC) work very nicely with the 530 nm LED used in our microscope's "RFP" light cube. And to provide a balanced reply, I should note that Zeiss offers a 530 nm LED fluorescence module, as do other companies (e.g., Leica, CoolLED, Fraen). There are some notable exceptions to 530 or 590 nm coverage -- for example, DsRed2 would greatly benefit from excitation in the 550-560 nm range. Manufacturers of LED-based fluorescence instrumentation keep a sharp eye out for new LED's that can fill in these gaps. In the meantime, having 7 dedicated LED modules does cover a very wide range of fluorescence applications, as noted by Eric in his message below. Cheers, -- Mark Rand ********************** Mark Rand, Ph.D. Product Manager and Senior Applications Scientist Advanced Microscopy Group Office: (425) 368-0413 http://www.amgmicro.com > Regarding the LED light sources for fluorescent microscopy, we have been > using one of these for the past six months, and it is wonderful. The > lighting is bright, even, and it doesn't quench (photobleach) FITC as fast > as mercury lamps do. It is easy to align, and the expected life of the LEDs > is at least 10,000 hours. > > I used mercury lamps for many years, and they were fun for those of us who > like to tinker with instruments, but overall, they are a pain in the neck to > use every day. About two years ago, we bought a new Nikon 50i microscope, > which is one of the best clinical microscopes I have ever used. > Unfortunately, the Nikon rep talked us into the EXFO metal halide light > source. This looked good in the demo, but it has spent more time being > repaired than working in our lab. Right now it is back at Nikon while they > try to figure out what is wrong with it. > > The LED light source that we are currently using is from a company called > Fraen. They are an Italian company, but they have an office in the US. The > unit is small, easy to install, easy to align, and gives illumination > equivalent to a 100 watt mercury lamp. > > Merced said that there are no LEDs available for red wavelengths, but this > must just be true for Zeiss. Fraen has LED modules that cover virtually all > of the fluorochromes that I have ever heard of. They have one for red > wavelengths that will work with TRITC, Cy3, Texas Red, Alexa Fluor 633, > Alexa Fluor 647, and even Cy5, which has maximum excitation at about 650 nm, > well into the red range. > > You do need to have different LEDs for various fluorochromes, but Fraen has > 7 modules available, and for practical terms, about 4 of them would cover > everything from DAPI to Cy5. They are easy to interchange on the Fraen > unit. > > I don't work for any of the companies mentioned here, nor do I receive any > compensation for saying nice things about their products. I just like their > products. > > Eric Hoy > > =============================================== > Eric S. Hoy, Ph.D., SI(ASCP) > Clinical Associate Professor > Department of Medical Laboratory Sciences > The University of Texas Southwestern Medical Center > Dallas, Texas > Email: Eric.Hoy <@t> UTSouthwestern.edu > =============================================== > > > Message: 1 > Date: Wed, 09 Sep 2009 13:43:45 -0400 > From: Merced M Leiker buffalo.edu> > Subject: Re: [Histonet] mercury vs led lights > To: Nejat Yilmaz mersin.edu.tr>, > histonet <@t> lists.utsouthwestern.edu > Message-ID: <6276F2A000238523BC2681F5 <@t> CDYwxp1931.ad.med.buffalo.edu> > Content-Type: text/plain; charset=us-ascii; format=flowed > > I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 > (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor > 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted > microscope we have LEDs and we also have a mercury lamp to cover the red > range. The Zeiss switches nicely and very smoothly between the light > sources during viewing and image acquisition. > > Sorry for the highly untechnical description, but that is all I know about > it. :-) From jclark <@t> pcnm.com Fri Sep 11 13:08:42 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Sep 11 13:08:47 2009 Subject: [Histonet] RE: Antibody Validation Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C010D3887@mail.pcnm.com> ------------------------------ We work out the specifics of the protocol for each new antibody with variables such as dilution, heat or enzyme retrieval, detection system and antibody incubation times based on the manufacturer's recommendations in the insert for the antibody. We do this testing on known positive tissue for that particular marker. Once we have the protocol figured out, than we run the second part of the validation using the newly developed protocol with several cases positive for that marker (usually 10 - 15) with varying degrees of positivity if possible. We also run 10 - 15 cases of known negative tissue types to make sure our protocol is not going to give us any false positivity. If we change any part of our routine IHC protocol, such as a new retrieval buffer or antibody diluent etc., than we have to revalidate each antibody we plan to use with the new reagent and record that we are getting consistent results with the product it was originally validated with. If the results are comparable, we record the data and put the new product into use. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 9 Date: Fri, 11 Sep 2009 08:20:43 -0700 (PDT) From: Akemi Allison-Tacha Subject: [Histonet] antibody validation To: histonet Message-ID: <332045.51983.qm@web31304.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation.? I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC R&D for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab.? I would like your assistance and input on how you are validating new antibodies. ? Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases.? Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases).? Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation.? I am curious how you approach validation.? Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com From LRaff <@t> uropartners.com Fri Sep 11 13:16:47 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Sep 11 13:16:53 2009 Subject: FW: [Histonet] Re: mercury vs led lights **Commercial Response** Message-ID: Is anyone using LED for UroVysion? Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Rand Sent: Friday, September 11, 2009 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: mercury vs led lights **Commercial Response** Full disclosure: My company manufactures an LED fluorescence microscope :-) Merced's information from the Zeiss rep is partially correct and needs some clarification. There is a "gap" in the spectrum of available LED's from 530 to 590 nm that would be powerful enough for fluorescence excitation. However, there aren't many fluorophores in that range that can't be covered by either 530 or 590 nm excitation. In fact, the examples cited (Alexa Fluor 555, Cy3, and TRITC) work very nicely with the 530 nm LED used in our microscope's "RFP" light cube. And to provide a balanced reply, I should note that Zeiss offers a 530 nm LED fluorescence module, as do other companies (e.g., Leica, CoolLED, Fraen). There are some notable exceptions to 530 or 590 nm coverage -- for example, DsRed2 would greatly benefit from excitation in the 550-560 nm range. Manufacturers of LED-based fluorescence instrumentation keep a sharp eye out for new LED's that can fill in these gaps. In the meantime, having 7 dedicated LED modules does cover a very wide range of fluorescence applications, as noted by Eric in his message below. Cheers, -- Mark Rand ********************** Mark Rand, Ph.D. Product Manager and Senior Applications Scientist Advanced Microscopy Group Office: (425) 368-0413 http://www.amgmicro.com > Regarding the LED light sources for fluorescent microscopy, we have been > using one of these for the past six months, and it is wonderful. The > lighting is bright, even, and it doesn't quench (photobleach) FITC as fast > as mercury lamps do. It is easy to align, and the expected life of the LEDs > is at least 10,000 hours. > > I used mercury lamps for many years, and they were fun for those of us who > like to tinker with instruments, but overall, they are a pain in the neck to > use every day. About two years ago, we bought a new Nikon 50i microscope, > which is one of the best clinical microscopes I have ever used. > Unfortunately, the Nikon rep talked us into the EXFO metal halide light > source. This looked good in the demo, but it has spent more time being > repaired than working in our lab. Right now it is back at Nikon while they > try to figure out what is wrong with it. > > The LED light source that we are currently using is from a company called > Fraen. They are an Italian company, but they have an office in the US. The > unit is small, easy to install, easy to align, and gives illumination > equivalent to a 100 watt mercury lamp. > > Merced said that there are no LEDs available for red wavelengths, but this > must just be true for Zeiss. Fraen has LED modules that cover virtually all > of the fluorochromes that I have ever heard of. They have one for red > wavelengths that will work with TRITC, Cy3, Texas Red, Alexa Fluor 633, > Alexa Fluor 647, and even Cy5, which has maximum excitation at about 650 nm, > well into the red range. > > You do need to have different LEDs for various fluorochromes, but Fraen has > 7 modules available, and for practical terms, about 4 of them would cover > everything from DAPI to Cy5. They are easy to interchange on the Fraen > unit. > > I don't work for any of the companies mentioned here, nor do I receive any > compensation for saying nice things about their products. I just like their > products. > > Eric Hoy > > =============================================== > Eric S. Hoy, Ph.D., SI(ASCP) > Clinical Associate Professor > Department of Medical Laboratory Sciences > The University of Texas Southwestern Medical Center > Dallas, Texas > Email: Eric.Hoy <@t> UTSouthwestern.edu > =============================================== > > > Message: 1 > Date: Wed, 09 Sep 2009 13:43:45 -0400 > From: Merced M Leiker buffalo.edu> > Subject: Re: [Histonet] mercury vs led lights > To: Nejat Yilmaz mersin.edu.tr>, > histonet <@t> lists.utsouthwestern.edu > Message-ID: <6276F2A000238523BC2681F5 <@t> CDYwxp1931.ad.med.buffalo.edu> > Content-Type: text/plain; charset=us-ascii; format=flowed > > I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5 > (far-red) fluorophores, but they cannot make them in the red (Alexa Fluor > 555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted > microscope we have LEDs and we also have a mercury lamp to cover the red > range. The Zeiss switches nicely and very smoothly between the light > sources during viewing and image acquisition. > > Sorry for the highly untechnical description, but that is all I know about > it. :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET NOD32 Antivirus, version of virus signature database 4417 (20090911) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4417 (20090911) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From scampbell <@t> celligent.net Fri Sep 11 14:12:43 2009 From: scampbell <@t> celligent.net (Campbell, Sharon) Date: Fri Sep 11 14:13:17 2009 Subject: [Histonet] Weck Blade handles Message-ID: Hello Histonetters, I am looking for info on any company that carries the Weck handles. I have blades that will work on this handle but the company that we get these from does not have the handles. Thanks for any help. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line scampbell@celligent.net From asmith <@t> mail.barry.edu Fri Sep 11 15:28:53 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 11 15:29:06 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: Theoretically, the fumes of HCl can react with the formaldehyde fumes to produce bis-chloromethyl ether, which is twice as toxic as osmium tetroxide. The yield from this reaction at 1 Atm is so small that I don't think I would worry about it unless I had open containers in a closed space. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ti fei Sent: Friday, September 11, 2009 10:23 AM To: Riesen, Rebecca; histonet Subject: Re:[Histonet] formalin storage never store everything in one cabinet. As far as i know, HCl and formalin should not be stored together. If any of you know why... ------------------ Original ------------------ From: "Riesen, Rebecca"; Date: Fri, Sep 11, 2009 10:09 PM To: "histonet"; Subject: [Histonet] formalin storage Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From macveigh <@t> usc.edu Fri Sep 11 15:55:25 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Sep 11 15:55:17 2009 Subject: [Histonet] Overstained Sirius Red... Message-ID: <008701ca3322$2f809120$5c237d80@DFS66DD1> Hi everybody, I have been doing Sirius Red for a while with good luck. Today we made fresh solutions and after the stain was done, the sections (rat liver) are too red. They are positive, the fibrosis is bright pink, but the background is pink as well. I got a slide back to water and distained for 2 more minutes with no luck. The only difference is the source of the saturated picric acid. On the old bottle the manufacturer is listed as Rowley Biochemical Institute (worked great) and the new one is Ricca Chemical CO. Could this be the problem? What can I do to correct this in the future? I have now 500ml of stain which is not optimal. Michelle Aloni USC School of Medicine GI Liver Department LA, CA From asmith <@t> mail.barry.edu Fri Sep 11 16:15:39 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 11 16:15:50 2009 Subject: [Histonet] formalin storage In-Reply-To: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> References: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC> Message-ID: Formaldehyde is flammable; formalin is not. Above 122 degrees Fahrenheit enough formaldehyde evaporates from formalin to create a modest fire hazard in the fumes just above the liquid. Try this: pour 3 ml of formalin (37% formaldehyde) into a watch glass under a fume hood (fan off). Touch a match to it. The match will flare briefly in the fumes and that is all (unless the temperature is above 122 F). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Warren Sent: Friday, September 11, 2009 9:25 AM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Fri Sep 11 16:21:50 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 11 16:22:24 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: Additionally, we have been told that the reaction product is also a very potent carcinogen and that is the reason why you are supposed to thoroughly rinse tissue free of unbound formalin prior to immersion in HCl based decalcification agents and once again when removing from decal and placing the tissue back into formalin. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, September 11, 2009 3:29 PM To: 'ti fei' Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: Re:[Histonet] formalin storage Theoretically, the fumes of HCl can react with the formaldehyde fumes to produce bis-chloromethyl ether, which is twice as toxic as osmium tetroxide. The yield from this reaction at 1 Atm is so small that I don't think I would worry about it unless I had open containers in a closed space. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ti fei Sent: Friday, September 11, 2009 10:23 AM To: Riesen, Rebecca; histonet Subject: Re:[Histonet] formalin storage never store everything in one cabinet. As far as i know, HCl and formalin should not be stored together. If any of you know why... ------------------ Original ------------------ From: "Riesen, Rebecca"; Date: Fri, Sep 11, 2009 10:09 PM To: "histonet"; Subject: [Histonet] formalin storage Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From linresearch <@t> comcast.net Sat Sep 12 11:48:52 2009 From: linresearch <@t> comcast.net (linresearch@comcast.net) Date: Sat Sep 12 11:48:55 2009 Subject: [Histonet] NMDAR & c-fos Abs Message-ID: <33355072.1518311252774132537.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net> Hello, Dose anyone know of reliable souces for the 2 ABs above that give consistnet results? I need to use them?the NMDAR1 Ab on?PFFE rat tissues and the c-fos on either frozen or PFFE tissues. I have tried one NMDAR1 ab with inconsistent results, one batch works well and the next batch does not give the same results using the same protocol. Thanks, lin From carl.hobbs <@t> kcl.ac.uk Sat Sep 12 13:42:10 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Sep 12 13:42:25 2009 Subject: [Histonet] Re: mercury vs led lights **Commercial Response** Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A5A@KCL-MAIL04.kclad.ds.kcl.ac.uk> Thanks for adding a response. However, I am a old Techie who does not understand what you are saying. My apologies. I use Alexa 488, 594 and also Hoechst to stain nuclei ( red, green , blue) So, if I changed from MY Xcite system, to LEDs, can I be assured that those three flourochromes I use will be equally detectable? What would I need to do to change to LEDs? What do I gain? I would be most grateful for further info, in easy language......you are very welcome to email me personally. Thanks. From tpodawiltz <@t> lrgh.org Sat Sep 12 21:31:38 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Sat Sep 12 21:35:03 2009 Subject: [Histonet] formalin storage In-Reply-To: <01A34750423B874399B8AF6674D90A270454782E@VHAV01MSGA1.v01.med.va.gov> References: <91ACB1A4A8904A2893BD8A6A8C6261DE@ownerPC>, <01A34750423B874399B8AF6674D90A270454782E@VHAV01MSGA1.v01.med.va.gov> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38890A62@LRGHEXVS1.practice.lrgh.org> I think the 1 gallon per 100 square feet is OSHA. What you have to make sure not to exceed the amount your cabinet is rated. for. We do not store are 10 % in a flammable cabinet. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. [Tina.Hayes@va.gov] Sent: Friday, September 11, 2009 9:37 AM To: Jean Warren; Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] formalin storage WE do not store our 10 NBF in flammable storage. However, Could you tell me where you found this direction of storing only 1 gallon per 100 sq. feet in a flammable cabinet, and 1 gallon outside? More specific than NFPA. I have been unable to find that anywhere, our safety folks tell us that if they are in a flammable cabinet the amount per room size doesn't matter. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Warren Sent: Friday, September 11, 2009 9:25 AM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, "How can something that is almost 90% water be a combustion hazard?" ----- Original Message ----- From: "Riesen, Rebecca" To: Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not "flammable" but that it IS "combustible". Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from "NA / >200F / 122F to 185F". The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our "bulk" storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From AnthonyH <@t> chw.edu.au Sun Sep 13 18:15:44 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 13 18:15:56 2009 Subject: [Histonet] Re: Permount question In-Reply-To: Message-ID: It is possible that the mountant was too thick and so xylene was added to thin it? It would save the lab money in mountant costs but there is a possibility that with less mountant on the slide you may have shrinkage of the mountant causing eventual cracking of the mountant and possible detachment of the coverslip. I would review a sample of slides one and two years old and record the quality (another audit!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Saturday, 12 September 2009 12:30 AM To: Leslie Chen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Permount question Just so a stereotype doesn't get started here...I was 1st taught in the (straight) use of Permount by such an Asian technician (who couldn't speak English, but was such a wonderful teacher I learned more from watching her than anything!) :-) --On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen wrote: > Thanks for all the replies, I guess these weird Asian technicians who > are highly looked up to taught me something wrong and it's been > propagated in the department. Thanks again! > > On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen > wrote: > >> Hello, >> Did anyone ever use Permount straight without cutting it with some >> solvent? Journal articles mention using Permount to coverslip, and >> the post-doc I work with thinks that it isn't diluted. >> >> I think maybe people just universally "know" that it should be >> diluted so it isn't mentioned in the Materials and Methods. Any >> thoughts? >> >> Leslie >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Sep 13 18:35:33 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 13 18:35:41 2009 Subject: [Histonet] antibody validation In-Reply-To: <332045.51983.qm@web31304.mail.mud.yahoo.com> Message-ID: Our procedure is: Read the data sheet for the antibody (recommended dilution, any pre-treatment) Read references that use the same antibody/clone (what should stain, what internal controls, their staining conditions) Obtain a known, or suspected, positive control and titre the antibody using recommended pre-treatment. Start with the titre recommended by the data sheet or from the literature. Using the optimised conditions stain a variety of tissues or lesions that should be positive as well as those lesions that should be negative (especially those that enter into the differential diagnosis) Record the results in your lab notebook and YOU ARE Done but, Continue to monitor the antibody's performance - this is important. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Saturday, 12 September 2009 1:21 AM To: histonet Subject: [Histonet] antibody validation Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation.? I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC R&D for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab.? I would like your assistance and input on how you are validating new antibodies. ? Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases.? Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases).? Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation.? I am curious how you approach validation.? Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From sarah_tarran <@t> wmi.usyd.edu.au Sun Sep 13 17:22:21 2009 From: sarah_tarran <@t> wmi.usyd.edu.au (Sarah Tarran) Date: Sun Sep 13 19:03:40 2009 Subject: [Histonet] Re: Polarizing filters (jstaruk) In-Reply-To: <20090911132606.0C10314755B@seive.med.usyd.edu.au> References: <20090911132606.0C10314755B@seive.med.usyd.edu.au> Message-ID: <44358.192.195.170.5.1252880541.squirrel@www.wmi.usyd.edu.au> Hi James, We have a Leica inverted microscope and just use polarising filters that are meant for cameras to look at our Sirius red stains (one is a cannon 58mm screw-in filter UV x1, the other we borrow from our audio-vision department so I am not sure what it is). We tape one directly below the stage and put one immediately above the slide. We then rotate the one above the slide until we have a black background and the collagen is shining yellow. If you get desperate, you can always use polarised sunglasses to have a look at your slides - not ideal but good for a quick look. Hope this helps! Sarah > > > Message: 13 > Date: Thu, 10 Sep 2009 16:46:38 -0400 > From: "jstaruk" > Subject: [Histonet] Polarizing filters > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Does anyone know where I can find the two appropriate filters (lenses) > needed to polarize the congo red and Sirius red stains? I have an Olympus > CH-2 that needs to be fitted. I understand I need a "polarizer" lens and > an > "analyzer" lens. Are these two different lenses or the same lens, just in > different locations on the microscope? > > Thank you > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com From Susan.Walzer <@t> HCAHealthcare.com Mon Sep 14 02:11:10 2009 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Sep 14 02:14:08 2009 Subject: [Histonet] formalin storage In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AC440F23F@FWDCWPMSGCMS09.hca.corpad.net> I think HCl and formalin when combined form a carcinogen, at least that is what I was taught many years ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ti fei Sent: Friday, September 11, 2009 10:23 AM To: Riesen, Rebecca; histonet Subject: Re:[Histonet] formalin storage never store everything in one cabinet. As far as i know, HCl and formalin should not be stored together. If any of you know why... ------------------ Original ------------------ From: "Riesen, Rebecca"; Date: Fri, Sep 11, 2009 10:09 PM To: "histonet"; Subject: [Histonet] formalin storage Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca Rebecca.Riesen@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Sep 14 02:14:16 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Sep 14 02:14:20 2009 Subject: [Histonet] type of paraffin and polymer Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07727A97@wahtntex2.waht.swest.nhs.uk> Ester Wax Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: 11 September 2009 17:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] type of paraffin and polymer Dear Histonetters, I quite need answer as soon as it possible, PLEASE! I am working with mouse tissue and tumors. What type of paraffin is the best for processing and embedding to cut 4? nice sections? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Mon Sep 14 08:19:26 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Sep 14 08:21:27 2009 Subject: [Histonet] AAVLD Histo sample block & slide retention policy In-Reply-To: References: <556B13AFEFCF4E6F87A234C8E2B31A93@auxs.umn.edu>, Message-ID: I disagree, the slide is what was used to make the diagnosis. The block may not be a good representation of the actual slide, it may could have been cut so that what is on the original slide is no longer in the block. In Surgical path we keep our slides indefinitely and our blocks for 10 years. Cheryl A. Miller HT ASCP Histology Supervisor Physcians Laboratory, P.C. 402 493 0403 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn [Lynn.Burton@Illinois.gov] Sent: Friday, September 11, 2009 10:35 AM To: Jan Shivers; histonet Subject: RE: [Histonet] AAVLD Histo sample block & slide retention policy I read our SOP's on storage and referred to Dr. webb, our pathologist. His feeling was that they, AAVLD, leave that up to each lab depending on the state statutes regarding law suits. Our SOP says seven years on blocks and nine years on slides. I would agree with you that as long as you have blocks, the slides would be less important. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Fri 9/11/2009 9:21 AM To: histonet Subject: [Histonet] AAVLD Histo sample block & slide retention policy For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From leiker <@t> buffalo.edu Mon Sep 14 08:59:35 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Sep 14 08:59:41 2009 Subject: [Histonet] Re: Permount question In-Reply-To: References: Message-ID: <82387741DFFFACDA16045D94@CDYwxp1931.ad.med.buffalo.edu> I do recall having to do that in the past; if the Permount bottle had been left open in the hood and became too viscous we would add just enough xylene to get it flowing again. Didn't happen often, if you're careful to keep the bottle closed when not in use. Regards, Merced --On Monday, September 14, 2009 9:15 AM +1000 Tony Henwood wrote: > It is possible that the mountant was too thick and so xylene was added > to thin it? > > It would save the lab money in mountant costs but there is a possibility > that with less mountant on the slide you may have shrinkage of the > mountant causing eventual cracking of the mountant and possible > detachment of the coverslip. > > I would review a sample of slides one and two years old and record the > quality (another audit!) > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M > Leiker > Sent: Saturday, 12 September 2009 12:30 AM > To: Leslie Chen; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: Permount question > > > Just so a stereotype doesn't get started here...I was 1st taught in the > (straight) use of Permount by such an Asian technician (who couldn't > speak > English, but was such a wonderful teacher I learned more from watching > her > than anything!) :-) > > > --On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen > wrote: > >> Thanks for all the replies, I guess these weird Asian technicians who >> are highly looked up to taught me something wrong and it's been >> propagated in the department. Thanks again! >> >> On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen >> wrote: >> >>> Hello, >>> Did anyone ever use Permount straight without cutting it with some >>> solvent? Journal articles mention using Permount to coverslip, and >>> the post-doc I work with thinks that it isn't diluted. >>> >>> I think maybe people just universally "know" that it should be >>> diluted so it isn't mentioned in the Materials and Methods. Any >>> thoughts? >>> >>> Leslie >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The > Childrens Hospital at Westmead accepts no liability for any consequential > damage resulting from email containing computer viruses. > ********************************************************************** > > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From shshaw <@t> WPI.EDU Mon Sep 14 09:07:18 2009 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Mon Sep 14 09:06:26 2009 Subject: [Histonet] DNA Extraction Message-ID: Hello Histo land, Does anyone know how to extract DNA from formalin fixed paraffin embedded tissue? Thanks, Sharon WPI From kblack <@t> digestivehlth.com Mon Sep 14 09:40:58 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Mon Sep 14 09:41:08 2009 Subject: [Histonet] AAVLD Histo sample block & slide retention policy References: <556B13AFEFCF4E6F87A234C8E2B31A93@auxs.umn.edu>, Message-ID: <8758C1EE74874374BA7D0107B8C228F9@digestivehlth.com> CLIA (feds) require blocks to be retained 2 years and slides to be retained 10 years. Space usually determines how long beyond that they are kept. We keep a "Cancer" file of slides and blocks that are retained indefinitely. K Black Digestive Health Specialists Tacoma, WA ----- Original Message ----- From: "Cheri Miller" To: "Burton, Lynn" ; "Jan Shivers" ; "histonet" Sent: Monday, September 14, 2009 6:19 AM Subject: RE: [Histonet] AAVLD Histo sample block & slide retention policy > I disagree, the slide is what was used to make the diagnosis. The block > may not be a good representation of the actual slide, it may could have > been cut so that what is on the original slide is no longer in the block. > In Surgical path we keep our slides indefinitely and our blocks for 10 > years. > > Cheryl A. Miller HT ASCP > Histology Supervisor > Physcians Laboratory, P.C. > 402 493 0403 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn > [Lynn.Burton@Illinois.gov] > Sent: Friday, September 11, 2009 10:35 AM > To: Jan Shivers; histonet > Subject: RE: [Histonet] AAVLD Histo sample block & slide retention policy > > I read our SOP's on storage and referred to Dr. webb, our pathologist. His > feeling was that they, AAVLD, leave that up to each lab depending on the > state statutes regarding law suits. Our SOP says seven years on blocks and > nine years on slides. I would agree with you that as long as you have > blocks, the slides would be less important. > > Lynn Burton > Lab Assoc. I > Animal Disease Lab > Galesburg, Il 61401 > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers > Sent: Fri 9/11/2009 9:21 AM > To: histonet > Subject: [Histonet] AAVLD Histo sample block & slide retention policy > > > > For veterinary diagnostic lab personnel: > > We're having a difference of opinion here over how long our Histo slides > need to be retained. It was my understanding that all paraffin blocks and > stained slides need to be retained for 7 years. Others here believe that > as long as the blocks are retained, it's not necessary to retain the > stained slides past a year or two. Storage space might be a problem in > the near future. > > I've looked through the AAVLD Essential Requirements, but cannot find a > definitive answer to this question. Can someone help me out with the > correct answer? We're writing a sample storage SOP for each lab section > today, and I'd like to get an answer as soon as possible. > > Thanks in advance, > Jan Shivers > UMN VDL > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon Sep 14 09:43:43 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Sep 14 09:43:50 2009 Subject: [Histonet] AAVLD Histo sample block & slide retention policy In-Reply-To: <8758C1EE74874374BA7D0107B8C228F9@digestivehlth.com> Message-ID: CAP requires 10 years for each. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Konni Black Sent: Monday, September 14, 2009 10:41 AM To: Cheri Miller; Burton, Lynn; Jan Shivers; histonet Subject: Re: [Histonet] AAVLD Histo sample block & slide retention policy CLIA (feds) require blocks to be retained 2 years and slides to be retained 10 years. Space usually determines how long beyond that they are kept. We keep a "Cancer" file of slides and blocks that are retained indefinitely. K Black Digestive Health Specialists Tacoma, WA ----- Original Message ----- From: "Cheri Miller" To: "Burton, Lynn" ; "Jan Shivers" ; "histonet" Sent: Monday, September 14, 2009 6:19 AM Subject: RE: [Histonet] AAVLD Histo sample block & slide retention policy > I disagree, the slide is what was used to make the diagnosis. The block > may not be a good representation of the actual slide, it may could have > been cut so that what is on the original slide is no longer in the block. > In Surgical path we keep our slides indefinitely and our blocks for 10 > years. > > Cheryl A. Miller HT ASCP > Histology Supervisor > Physcians Laboratory, P.C. > 402 493 0403 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn > [Lynn.Burton@Illinois.gov] > Sent: Friday, September 11, 2009 10:35 AM > To: Jan Shivers; histonet > Subject: RE: [Histonet] AAVLD Histo sample block & slide retention policy > > I read our SOP's on storage and referred to Dr. webb, our pathologist. His > feeling was that they, AAVLD, leave that up to each lab depending on the > state statutes regarding law suits. Our SOP says seven years on blocks and > nine years on slides. I would agree with you that as long as you have > blocks, the slides would be less important. > > Lynn Burton > Lab Assoc. I > Animal Disease Lab > Galesburg, Il 61401 > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers > Sent: Fri 9/11/2009 9:21 AM > To: histonet > Subject: [Histonet] AAVLD Histo sample block & slide retention policy > > > > For veterinary diagnostic lab personnel: > > We're having a difference of opinion here over how long our Histo slides > need to be retained. It was my understanding that all paraffin blocks and > stained slides need to be retained for 7 years. Others here believe that > as long as the blocks are retained, it's not necessary to retain the > stained slides past a year or two. Storage space might be a problem in > the near future. > > I've looked through the AAVLD Essential Requirements, but cannot find a > definitive answer to this question. Can someone help me out with the > correct answer? We're writing a sample storage SOP for each lab section > today, and I'd like to get an answer as soon as possible. > > Thanks in advance, > Jan Shivers > UMN VDL > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From joseph-galbraith <@t> uiowa.edu Mon Sep 14 10:30:21 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Sep 14 10:30:26 2009 Subject: [Histonet] DNA Extraction In-Reply-To: References: Message-ID: Sharon: Here is just one of many such protocols published recently. Commercial kits are available from molecular diagnostic suppliers (we use a Qiagen kit for example - there are certainly numerous others). http://www.protocol-online.org/prot/Protocols/DNA-Extraction-from-Archiv al-Formalin-fixed--Paraffin-embedded-Tissue-Sections-3159.html Joe 380 MRC 4-4737 (voice) 3-3482 (fax) pager 131-1170 joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Monday, September 14, 2009 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DNA Extraction Hello Histo land, Does anyone know how to extract DNA from formalin fixed paraffin embedded tissue? Thanks, Sharon WPI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Mon Sep 14 11:34:29 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Sep 14 11:34:33 2009 Subject: [Histonet] Company email Message-ID: <642167.503.qm@web57804.mail.re3.yahoo.com> I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with?a credit card. I placed the order on?9/2 and have been fighting with them ever since. Ridiculous!?Today they were standing by their policy of not accepting?my email address as a business address so I'm taking?my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with.? ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From mpence <@t> grhs.net Mon Sep 14 11:38:54 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Sep 14 11:38:57 2009 Subject: [Histonet] Company email In-Reply-To: <642167.503.qm@web57804.mail.re3.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3C0C@is-e2k3.grhs.net> Why would you think your email address is your business address? Just have to ask? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with?a credit card. I placed the order on?9/2 and have been fighting with them ever since. Ridiculous!?Today they were standing by their policy of not accepting?my email address as a business address so I'm taking?my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with.? ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Sep 14 11:43:53 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Sep 14 11:44:42 2009 Subject: [Histonet] Company email References: <661949901A768E4F9CC16D8AF8F2838C017A3C0C@is-e2k3.grhs.net> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2B40@fhosxchmb006.ADVENTISTCORP.NET> Where were you going to have the product delivered?...... Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mike Pence Sent: Mon 9/14/2009 12:38 PM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Company email Why would you think your email address is your business address? Just have to ask? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From joseph-galbraith <@t> uiowa.edu Mon Sep 14 12:05:49 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Sep 14 12:06:01 2009 Subject: [Histonet] Company email In-Reply-To: <642167.503.qm@web57804.mail.re3.yahoo.com> References: <642167.503.qm@web57804.mail.re3.yahoo.com> Message-ID: Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourname@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with?a credit card. I placed the order on?9/2 and have been fighting with them ever since. Ridiculous!?Today they were standing by their policy of not accepting?my email address as a business address so I'm taking?my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with.? ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Mon Sep 14 12:33:22 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Sep 14 12:33:27 2009 Subject: [Histonet] Company email In-Reply-To: References: <642167.503.qm@web57804.mail.re3.yahoo.com> Message-ID: <234842.34658.qm@web57804.mail.re3.yahoo.com> I've jumped through hoops already?and made?a dozen calls to?the company (they are refusing) to no evail so I suppose "hurt" is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: "Galbraith, Joe" To: Sheila Haas Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed.? This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly.? These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses.? They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc.? For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem.? You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue.? Look to see whose secure site you are transferred to when you go to pay for your purchase on-line.? That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourname@yourcompany.com).? In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach.? If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt.? Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed.? That may help them be less concerned.? Best of luck. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with?a credit card. I placed the order on?9/2 and have been fighting with them ever since. Ridiculous!?Today they were standing by their policy of not accepting?my email address as a business address so I'm taking?my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with.? ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Sep 14 12:35:36 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Sep 14 12:35:41 2009 Subject: [Histonet] Company email In-Reply-To: References: <642167.503.qm@web57804.mail.re3.yahoo.com> Message-ID: <8903.44035.qm@web1112.biz.mail.sk1.yahoo.com> Sheila, Joe is correct about the generic emails such as yahoo, gmail, etc. Look into creating your own website which will then come with email in the you@yourcompany.com?format. Websites can be hosted for as little as $10-12/month and the name searched for conflicts.?Some even has easy, free software to design?the website yourself. Paula ________________________________ From: "Galbraith, Joe" To: Sheila Haas Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 12:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed.? This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly.? These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses.? They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc.? For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem.? You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue.? Look to see whose secure site you are transferred to when you go to pay for your purchase on-line.? That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourname@yourcompany.com).? In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach.? If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt.? Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed.? That may help them be less concerned.? Best of luck. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with?a credit card. I placed the order on?9/2 and have been fighting with them ever since. Ridiculous!?Today they were standing by their policy of not accepting?my email address as a business address so I'm taking?my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with.? ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Mon Sep 14 12:36:11 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Sep 14 12:36:25 2009 Subject: [Histonet] Company email In-Reply-To: <234842.34658.qm@web57804.mail.re3.yahoo.com> References: <642167.503.qm@web57804.mail.re3.yahoo.com> <234842.34658.qm@web57804.mail.re3.yahoo.com> Message-ID: Sometimes the best way to complain is with the "feet". Joe joseph-galbraith@uiowa.edu ________________________________ From: Sheila Haas [mailto:micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 12:33 PM To: Galbraith, Joe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email I've jumped through hoops already and made a dozen calls to the company (they are refusing) to no evail so I suppose "hurt" is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories ________________________________ From: "Galbraith, Joe" To: Sheila Haas Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourname@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Sep 14 12:41:06 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Sep 14 12:42:59 2009 Subject: [Histonet] Company email In-Reply-To: <642167.503.qm@web57804.mail.re3.yahoo.com> References: <642167.503.qm@web57804.mail.re3.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38890A67@LRGHEXVS1.practice.lrgh.org> Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From micropathlabs <@t> yahoo.com Mon Sep 14 12:49:09 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Sep 14 12:49:14 2009 Subject: [Histonet] Company email In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D38890A67@LRGHEXVS1.practice.lrgh.org> References: <642167.503.qm@web57804.mail.re3.yahoo.com> <38667E7FB77ECD4E91BFAEB8D98638631D38890A67@LRGHEXVS1.practice.lrgh.org> Message-ID: <265294.17996.qm@web57805.mail.re3.yahoo.com> Yes, it was a company card. They indicated payment wasn't the issue they needed the email address?for their records. Go figure! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: "Podawiltz, Thomas" To: Sheila Haas ; "histonet@lists.utsouthwestern.edu" Sent: Monday, September 14, 2009 1:41:06 PM Subject: RE: [Histonet] Company email Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL.? This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.? If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From akemiat3377 <@t> yahoo.com Mon Sep 14 12:53:30 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Sep 14 12:53:33 2009 Subject: [Histonet] antibody validation Thanks, but need Non-"U" also Message-ID: <355352.34611.qm@web31303.mail.mud.yahoo.com> Hi All, I would like to thank those of you who have responded to the e-mail below regarding antibody validation.? The information I received was pretty much what I expected from university IHC labs.? I would like to know what smaller IHC labs are doing for validation and re-validation when changes are made to their protocols. Thank you again for any and all responses, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com --- On Fri, 9/11/09, Akemi Allison-Tacha wrote: From: Akemi Allison-Tacha Subject: [Histonet] antibody validation To: "histonet" Date: Friday, September 11, 2009, 8:20 AM Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation.? I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC R&D for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab.? I would like your assistance and input on how you are validating new antibodies. ? Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases.? Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases).? Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation.? I am curious how you approach validation.? Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Sep 14 13:04:57 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Sep 14 13:06:47 2009 Subject: [Histonet] Company email In-Reply-To: <265294.17996.qm@web57805.mail.re3.yahoo.com> References: <642167.503.qm@web57804.mail.re3.yahoo.com> <38667E7FB77ECD4E91BFAEB8D98638631D38890A67@LRGHEXVS1.practice.lrgh.org>, <265294.17996.qm@web57805.mail.re3.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38890A68@LRGHEXVS1.practice.lrgh.org> Talk about being ridiculous. Almost sounds like Navy logic Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________ From: Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 1:49 PM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email Yes, it was a company card. They indicated payment wasn't the issue they needed the email address for their records. Go figure! Sheila Haas Laboratory Supervisor Micro Path Laboratories ________________________________ From: "Podawiltz, Thomas" To: Sheila Haas ; "histonet@lists.utsouthwestern.edu" Sent: Monday, September 14, 2009 1:41:06 PM Subject: RE: [Histonet] Company email Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From micropathlabs <@t> yahoo.com Mon Sep 14 13:13:57 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Sep 14 13:14:02 2009 Subject: [Histonet] Company email In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D38890A68@LRGHEXVS1.practice.lrgh.org> References: <642167.503.qm@web57804.mail.re3.yahoo.com> <38667E7FB77ECD4E91BFAEB8D98638631D38890A67@LRGHEXVS1.practice.lrgh.org>, <265294.17996.qm@web57805.mail.re3.yahoo.com> <38667E7FB77ECD4E91BFAEB8D98638631D38890A68@LRGHEXVS1.practice.lrgh.org> Message-ID: <196980.9613.qm@web57806.mail.re3.yahoo.com> Thanks to all for listening and the input. I knew it wasn't just me! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: "Podawiltz, Thomas" To: Sheila Haas ; "histonet@lists.utsouthwestern.edu" Sent: Monday, September 14, 2009 2:04:57 PM Subject: RE: [Histonet] Company email Talk about being ridiculous. Almost sounds like Navy logic ? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________ From: Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 1:49 PM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email Yes, it was a company card. They indicated payment wasn't the issue they needed the email address?for their records. Go figure! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: "Podawiltz, Thomas" To: Sheila Haas ; "histonet@lists.utsouthwestern.edu" Sent: Monday, September 14, 2009 1:41:06 PM Subject: RE: [Histonet] Company email Just curious, were you trying to use a company credit card? If not try setting up a paypal account that is link to your credit card. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL.? This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.? If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From TroyerDA <@t> EVMS.EDU Mon Sep 14 15:09:30 2009 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Mon Sep 14 15:10:19 2009 Subject: [Histonet] Company email References: <642167.503.qm@web57804.mail.re3.yahoo.com><234842.34658.qm@web57804.mail.re3.yahoo.com> Message-ID: <58142461040E184397658FEC96696A0D5482FA@romulus.evms.net> I imagine there are some regulations pertaining to lab supplies, chemicals etc post-9/11 also. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Galbraith, Joe Sent: Mon 9/14/2009 1:36 PM To: Sheila Haas Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Company email Sometimes the best way to complain is with the "feet". Joe joseph-galbraith@uiowa.edu ________________________________ From: Sheila Haas [mailto:micropathlabs@yahoo.com] Sent: Monday, September 14, 2009 12:33 PM To: Galbraith, Joe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email I've jumped through hoops already and made a dozen calls to the company (they are refusing) to no evail so I suppose "hurt" is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories ________________________________ From: "Galbraith, Joe" To: Sheila Haas Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourname@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet :: From Rcartun <@t> harthosp.org Tue Sep 15 08:31:18 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Sep 15 08:31:25 2009 Subject: [Histonet] CPT technical codes for FISH testing Message-ID: <4AAF5EE5.7400.0077.1@harthosp.org> What CPT codes are people using for technical billing for FISH testing performed on paraffin sections (e.g., HER2)? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From shshaw <@t> WPI.EDU Tue Sep 15 08:42:11 2009 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Tue Sep 15 08:41:20 2009 Subject: [Histonet] mice legs Message-ID: Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if anyone has do this would it be possible to share your protocol with me from decal to processing. Thanks, Sharon- WPI From derek.papalegis <@t> tufts.edu Tue Sep 15 08:51:10 2009 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Sep 15 08:51:15 2009 Subject: [Histonet] mice legs In-Reply-To: References: Message-ID: <4AAF9BCE.9010706@tufts.edu> Hi Sharon, I frequently get whole mouse legs submitted to me and decalcification doesn't break down the tissue. I use Cal-Rite as a decalcifier but any formic acid decal would be sufficient. Since formic acid is pretty forgiving, I decal the legs for 48-72 hours on a rotator. I change the solution after 24-48 hours and then place them back in decal for 24 hours. I have left bones in decal over the weekend and they have been fine. I have found that if you are focusing on the long bones, this is sufficient but if you are interested in seeing the ankles and feet, you should remove them from the leg and decal them longer. When processing, I use a longer program. Here is the program that I use: 70% 1 hr 80% 1 hr 95% 2 hr 95% 2.5 hr 100% 2.5hr 100% 3hr 100% 3hr xylene 1hr20min xylene 1hr20min xylene 1hr20min paraffin 1hr paraffin 1hr paraffin 1.5 hr paraffin 1.5 hr When processing, only process the bone samples with this program. If you batch other tissues in with the bones, the tissue will be over processed. Let me know if you have any more questions. Derek Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University Shaw, Sharon wrote: > Good Morning Histo World, > > I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if anyone has do this would it be possible to share your protocol with me from decal to processing. > > Thanks, > Sharon- WPI > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From GDawson <@t> dynacaremilwaukee.com Tue Sep 15 09:23:44 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Sep 15 09:23:50 2009 Subject: [Histonet] C3d In-Reply-To: <4AAF9BCE.9010706@tufts.edu> Message-ID: All, I have a request to work up a monoclonal C3d fluorescent stain (for frozen sections). Can anyone out there suggest a good one? I am currently running C4d & clinicians are asking for the C3d as well. Thank-you in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From Jackie.O'Connor <@t> abbott.com Tue Sep 15 10:13:59 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Sep 15 10:14:25 2009 Subject: [Histonet] mice legs In-Reply-To: References: Message-ID: I did a lot of IHC work with whole mouse legs where we had to pay close attention to the tibia, as well as the joint. When the mice were necropsied, I had the prosectors remove all the soft tissue and foot without disrupting the joint. They could do this easily with fresh tissue as opposed to after fixation. The leg bones were fixed for no more than 48 hours, and decalcified in home-brewed 5% formic acid overnight on a shaker table. I then trimmed them to expose the joint and bone marrow prior to processing on a routine program, about 45 minutes per station. Perfect ever single time. IHC was beautiful. Hope this helps. Jackie O' From: "Shaw, Sharon" To: "histonet@lists.utsouthwestern.edu" Date: 09/15/2009 08:46 AM Subject: [Histonet] mice legs Sent by: histonet-bounces@lists.utsouthwestern.edu Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if anyone has do this would it be possible to share your protocol with me from decal to processing. Thanks, Sharon- WPI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Sep 15 11:16:42 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Sep 15 11:16:49 2009 Subject: Fw: Re: [Histonet] antibody validation Thanks, but need Non-"U" also Message-ID: <952901.51895.qm@web31306.mail.mud.yahoo.com> Hi Cynthia, Thank you, this is exactly what I was looking for.? You are a rare jewel.? Especially for the size of your facility.? I am impressed!? I wish I would have received more feed-back from small labs, but that's OK.? I have been doing some pro-active sleuthing of local labs. After numerous conversations with my fellow histologists that do IHC, I am finding out what I have long suspected and have experienced while doing technical support and consulting.? The routine histology laboratory is multitasking all of their regular histology duties with the addition of IHC testing.? Pathologists do not want to lose the IHC revenue to a reference laboratory, so they bring on-board simple IHC panels, and send out their esoteric or prognostic markers to a well known reference laboratory.? Most histologists have been asked to bring on board IHC, without the proper educational background or tools.??? The average histologist has not had the opportunity to take continuing education classes, or even go on the histonet.? This may be due to lack of continuing education funds, or lack of interest; It is amazing that pathologists can go to their continuing education meetings, but don't support their histologists.? Unfortunately, these histologists do not understand the importance of standardization or validation steps.? When something goes bump in the night, they are unaware how to troubleshoot the problem.? This is unfortunate for the histologist, the pathologist and most of all, the patient.? One more reason for pathologists to support continuing education.? Hope to see you all at NSH! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com --- On Mon, 9/14/09, Cynthia Robinson wrote: From: Cynthia Robinson Subject: Re: [Histonet] antibody validation Thanks, but need Non-"U" also To: "Akemi Allison-Tacha" Date: Monday, September 14, 2009, 11:08 AM Hi, I work at 250 bed facility.? For the initial protocol workup we use the manufacturer recommended protocol and run an IHC TMA control block with 25 different tissues and tumors.? Once the pathologist has looked and responded I search for 5-10 cases with expected positive results and then a selection of 5-10 normal or negative tissues and run the protocol. Hope this is what you were wanting. Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> Akemi Allison-Tacha 9/14/2009 12:53 PM >>> Hi All, I would like to thank those of you who have responded to the e-mail below regarding antibody validation.? The information I received was pretty much what I expected from university IHC labs.? I would like to know what smaller IHC labs are doing for validation and re-validation when changes are made to their protocols. Thank you again for any and all responses, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com --- On Fri, 9/11/09, Akemi Allison-Tacha wrote: From: Akemi Allison-Tacha Subject: [Histonet] antibody validation To: "histonet" Date: Friday, September 11, 2009, 8:20 AM Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation.? I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC R&D for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab.? I would like your assistance and input on how you are validating new antibodies.???Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases.? Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases).? Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation.? I am curious how you approach validation.? Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas.crowell <@t> novartis.com Tue Sep 15 12:16:49 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Tue Sep 15 12:16:59 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 09/15/2009 and will not return until 09/16/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. From dgaupp <@t> tulane.edu Tue Sep 15 13:29:25 2009 From: dgaupp <@t> tulane.edu (Gaupp, Dina D ) Date: Tue Sep 15 13:29:29 2009 Subject: [Histonet] gastrocnemius muscle problems in paraffin Message-ID: <447056A67472B241A330A525B4AF7167018043B4@EX02.ad.tulane.edu> Hi All: I am having difficulty sectioning mouse gastro. muscle. They were brought to lab in 4-5mls(not enough fix) of 10%nbf for 3 days. Processed for over 8 hours(vacuum from 95% to Paraffin everyother station) . The tissue will not section. The white area appears once surfaced inidicating tissue was not well processed or fixed completely. Has anyone experience what the next step would be to do? Was the tissue not well infiltrated with paraffin for it not to section? Is the tissue overprocessed? Should I go back & reprocess with paraffin only? Any help........ Dina Dina D. Gaupp, BS, MT Senior Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu From carl.hobbs <@t> kcl.ac.uk Tue Sep 15 14:22:39 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Tue Sep 15 14:24:46 2009 Subject: [Histonet] ImmPACT DAB kit Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A6C@KCL-MAIL04.kclad.ds.kcl.ac.uk> Has anyone tested this? I would be very interested. Ta carl From disbrc <@t> shands.ufl.edu Tue Sep 15 15:50:27 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Tue Sep 15 15:50:42 2009 Subject: [Histonet] ISH and Bone Marrows question In-Reply-To: <18f6e1ac000bd731@TREND12.Shands.Local> References: <18f6e1ac000bd731@TREND12.Shands.Local> Message-ID: <4AAFC5D9.72AC.0059.0@shands.ufl.edu> Hi Histonetters! We have recently changed to Immunocal to decal bone marrow biopsies. Immuno stains performed on the decaled bone marrows look good. All In-situ hybridization negative controls, and Kappa and Lambda stains on decaled bone marrows have a blue artifact at the edges. We use the Ventana Benchmark. Our lymph node controls for ISH and all other ISH tissue do not have the edge artifact. Has anyone seen this artifact before and were you able to prevent it? Any help would be appreciated! Thanks! Elaine Dooley, HTL 352-265-0111 ext. 72117 From louise.renton <@t> gmail.com Wed Sep 16 02:57:30 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Sep 16 02:57:40 2009 Subject: [Histonet] mice legs In-Reply-To: References: Message-ID: No, I tend to use my own for work! sorry couldn'r resist midweek madness...............;-) On Tue, Sep 15, 2009 at 3:42 PM, Shaw, Sharon wrote: > Good Morning Histo World, > > I would like to know if anyone is working with mice legs, I have a PI that > I work with that wants to process the whole leg, the problem is I need to > decal it first and is wondering if the decal will break down the tissue, I > think it would he doesn't think so. And if anyone has do this would it be > possible to share your protocol with me from decal to processing. > > Thanks, > Sharon- WPI > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From godsgalnow <@t> aol.com Wed Sep 16 09:40:26 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Sep 16 09:40:45 2009 Subject: [Histonet] air quality in the lab Message-ID: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. From jcrawfor <@t> aerotek.com Wed Sep 16 09:50:57 2009 From: jcrawfor <@t> aerotek.com (Crawford, Jennifer) Date: Wed Sep 16 09:55:19 2009 Subject: [Histonet] Histotechs-Chicago, IL Message-ID: <571A823E0300F549BE86279A8723957703A3600B@ag00-exmbx04.allegisgroup.com> Good morning Histologists! A hospital that I am recruiting for in the Chicago area has 2 openings for Histotechs on a 1st and 2nd shift schedule, both positions are contract to hire and included medical, dental and vision benefits through Aerotek. The employer is looking for 3+ years of relevant experience and an ASCP certification is required. Please email me directly at jcrawfor@aerotek.com if you are interested or would like further details. Thank you for your time! Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From leiker <@t> buffalo.edu Wed Sep 16 10:11:58 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 16 10:12:03 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> References: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> Message-ID: <2EA840C640D520F1A4C7F23D@CDYwxp1931.ad.med.buffalo.edu> that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgalnow@aol.com wrote: > > Does anyone know what the regulations or requirements are for the air > turnover in the lab? > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From alyssa <@t> alliedsearchpartners.com Wed Sep 16 10:34:37 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Sep 16 10:34:41 2009 Subject: [Histonet] Job In FL Message-ID: Good Morning! Allied Search Partners is currently accepting resumes for our client in Melbourne, FL area. We are prescreening for the following: Position(s): Histotechnologists/Histotechnicians Shift: 1. One tech needed for Day Shift Full Time (8am-4:30pm) 2. One tech needed for Part Time Please submit resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are kept confidential* Be sure to visit our website to submit a job search request, refer a friend for $$Cash Bonus&&, and submit your resume to one of our career advisors. Other positions in Florida: Dover, FL (Evening and Overnight Shift Available) -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From rjbuesa <@t> yahoo.com Wed Sep 16 10:52:41 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 16 10:52:45 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <2EA840C640D520F1A4C7F23D@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <704853.37612.qm@web65707.mail.ac4.yahoo.com> From?the recesses of my sometimes foggy memory, the figure of 4 exchanges/hour has popped up. Perhaps somebody else has some other figure. Ren? J. --- On Wed, 9/16/09, Merced M Leiker wrote: From: Merced M Leiker Subject: Re: [Histonet] air quality in the lab To: godsgalnow@aol.com, histonet@lists.utsouthwestern.edu Date: Wednesday, September 16, 2009, 11:11 AM that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgalnow@aol.com wrote: > > Does anyone know what the regulations or requirements? are for the air > turnover in the lab? > > > >? Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Sep 16 11:17:57 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Sep 16 11:20:35 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> References: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38890A6F@LRGHEXVS1.practice.lrgh.org> Our last inspection our exchange rate tested out at 52/hour. We had increased from 26/hour because we could still had fumes in the lab. (CMS inspector could smell xylene from our stainer) For the life of me cannot remember what the min. rate is . Tom Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com [godsgalnow@aol.com] Sent: Wednesday, September 16, 2009 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air quality in the lab Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From JWeems <@t> sjha.org Wed Sep 16 12:23:45 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 16 12:23:49 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D38890A6F@LRGHEXVS1.practice.lrgh.org> References: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> <38667E7FB77ECD4E91BFAEB8D98638631D38890A6F@LRGHEXVS1.practice.lrgh.org> Message-ID: I seem to remember 12... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, September 16, 2009 12:18 To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] air quality in the lab Our last inspection our exchange rate tested out at 52/hour. We had increased from 26/hour because we could still had fumes in the lab. (CMS inspector could smell xylene from our stainer) For the life of me cannot remember what the min. rate is . Tom Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com [godsgalnow@aol.com] Sent: Wednesday, September 16, 2009 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air quality in the lab Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From BUCKINGHAM <@t> email.chop.edu Wed Sep 16 12:30:28 2009 From: BUCKINGHAM <@t> email.chop.edu (Carol Buckingham) Date: Wed Sep 16 12:30:57 2009 Subject: [Histonet] TISSUE FREEZING MEDIUM Message-ID: <4AB0E8740200007E0001361F@email.chop.edu> I'm having a great deal of trouble finding a tissue freezing medium that is anything like the original OCT -15-25. Since OCT is no longer available at different temperature uses, I cannot cut a good section in our cryostat(-25)without using freezing spray. Even after trying a few different brands of medium,without freezing spray, our sections are still folded,wavy,and all around not of good quality like we used to have. Most of the different brands are fine for a specimen that was just snap frozen and cut, as in a surgical frozen case, but not good for serial sectioning that may take place 1 to 2 days later, as in a renal bx.We have tried to cut on three different cryostats and had similar results.Surely there are those reading this that are having the same problem.Thanks in advance for your help. From Vickroy.Jim <@t> mhsil.com Wed Sep 16 13:07:17 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Sep 16 13:07:23 2009 Subject: [Histonet] need pneumocystis control blocks Message-ID: <24A4826E8EF0964D86BC5317306F58A53E3AC969ED@mmc-mail.ad.mhsil.com> Does any body know where we can get a few pneumocystis control blocks? Last time I needed a control block for AFB the members really came through. Hopefully someone will have extra pneumocystis blocks also. thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From carl.hobbs <@t> kcl.ac.uk Wed Sep 16 13:09:39 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Sep 16 13:10:53 2009 Subject: [Histonet] Re: ImmPACT DAB kit Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A6F@KCL-MAIL04.kclad.ds.kcl.ac.uk> Many thanks for the replies regarding this reagent. I delayed purchasing this as I was very skeptical: however, I can achieve a 1/5-10 fold dilution factor for my precious low affinity (? thus low dilution factor) Abs. I will not be using ImmPACT chromogen kit routinely as, for the vast majority of Abs, I do not find it neccessary. I have been informed ( courtesy of Histonetters who replied) that Dako offer a similarly sensitive kit. For the record, I routinely use a std manual stABCpx-DAB (DAB in-house prepared)detection system in a Research Lab with very little "clinical" time-constraints) carl From PMonfils <@t> Lifespan.org Wed Sep 16 13:12:38 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Sep 16 13:12:44 2009 Subject: [Histonet] Polarizing filters In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CE6@LSRIEXCH1.lsmaster.lifespan.org> The polarizer and analyzer are identical filters, and either of them can be used in either location. One must be between the light source and the slide being viewed. The other must be between the slide being viewed and your eye or camera. I place one filter directly on top of my illuminator. The other is in a filter slide in the microscope column, which can be pushed into the light beam or pulled out of it, but you can also place it directly on top of the slide. You rotate either filter to achieve the polarization effect. I rotate the lower one since the other one is not accessible. These filters cut down the light intensity substantially, so you should use them with maximum brightness of the illuminator, iris diaphram wide open, and with neutral density or any other kinds of filters removed from the light beam, including the blue filter if you normally use one. Polarizing filters can be purchased at any camera store, and some science supply companies sell them. Get good quality glass filters though, not cheap plastic ones. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk > Sent: Thursday, September 10, 2009 4:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Polarizing filters > > Does anyone know where I can find the two appropriate filters (lenses) > needed to polarize the congo red and Sirius red stains? I have an Olympus > CH-2 that needs to be fitted. I understand I need a "polarizer" lens and an > "analyzer" lens. Are these two different lenses or the same lens, just in > different locations on the microscope? > > Thank you > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From integrated.histo <@t> gmail.com Wed Sep 16 13:25:10 2009 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Sep 16 13:25:14 2009 Subject: [Histonet] Re: Air Quality Message-ID: <5d9104a30909161125q4fcc4addx565e3b854e807104@mail.gmail.com> Does anyone know where we can find written regulations regarding air quality in the lab? We start @ 330 am, but the HVAC system doesn't come on until 7. So we are working with just portable fans (or heaters in the winter) those first few hours. Cindy From godsgalnow <@t> aol.com Wed Sep 16 13:44:26 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Sep 16 13:44:39 2009 Subject: [Histonet] mib-1 Message-ID: <8CC0514DA122A7A-3CC4-76F5@webmail-d036.sysops.aol.com> I have to turn to the experts here.? I am having problems with the MIB-1.? I am having an issue with the patient samples working.? We use an alcohol based fixative....it has always worked until recently. I have tried everything I can think of to no avail.? I have decreased the titer, increased the incubation, tried a different retrieval solution, tried a different detection...nothing is working. Anyone else having any issues? From LBlack <@t> carilion.com Wed Sep 16 13:56:30 2009 From: LBlack <@t> carilion.com (Lisa S. Black) Date: Wed Sep 16 13:50:11 2009 Subject: [Histonet] Air changes per hour Message-ID: <20090916T145630Z_AA4400110000@carilion.com> OSHA's "Occupational Exposure to Hazardous Chemicals in Laboratories; Final Rule" (Federal Register January 31, 1990) went into effect May 1, 1990. Section C.4 (f) of Non-Mandatory Appendix A indicates that 4-12 room air changes per hours (ACH) of fresh air are normally adequate if local exhaust systems such as hoods are used as the primary method of control. From rjbuesa <@t> yahoo.com Wed Sep 16 15:07:23 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 16 15:07:28 2009 Subject: [Histonet] Re: Air Quality In-Reply-To: <5d9104a30909161125q4fcc4addx565e3b854e807104@mail.gmail.com> Message-ID: <685525.48387.qm@web65710.mail.ac4.yahoo.com> Regardless of the regulations, fans will not providce them so the real solution is to have HVAC system running when you start, or do they want to subject you to unsanitary conditions? Have the ever heard of an "OSHA intervention" or about their fines? Ren? J.? --- On Wed, 9/16/09, Cindy DuBois wrote: From: Cindy DuBois Subject: [Histonet] Re: Air Quality To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 16, 2009, 2:25 PM Does anyone know where we can find written regulations regarding air quality in the lab?? We start @ 330 am, but the HVAC system doesn't come on until 7.? So we are working with just portable fans (or heaters in the winter) those first few hours. Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Wed Sep 16 15:31:15 2009 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Sep 16 15:31:19 2009 Subject: [Histonet] hyaluronidase for IHC pretreatment Message-ID: <703819.5584.qm@web38008.mail.mud.yahoo.com> Hello, ? I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution.?Could anybody help me please? ? Best regards, ? Amy From mcauliff <@t> umdnj.edu Wed Sep 16 15:56:33 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Sep 16 15:54:57 2009 Subject: [Histonet] hyaluronidase for IHC pretreatment In-Reply-To: <703819.5584.qm@web38008.mail.mud.yahoo.com> References: <703819.5584.qm@web38008.mail.mud.yahoo.com> Message-ID: <4AB15101.1040401@umdnj.edu> You buy hyaluronidase (freeze-dried) from Sigma or some other vendor and make it up in the appropriate buffer. If my memory serves me, and there have been no major advances in the field, hyaluronidase comes in two flavors, one from bovine testis and one from Streptomyces sp. These are NOT the same so be sure of which one is appropriate for your work..I think the Strep variety is more specific? Geoff Amy Lee wrote: > Hello, > > I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please? > > Best regards, > > Amy > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Stella.Argyriou <@t> leica-microsystems.com Wed Sep 16 20:30:46 2009 From: Stella.Argyriou <@t> leica-microsystems.com (Stella Argyriou) Date: Wed Sep 16 20:31:01 2009 Subject: [Histonet] Peloris tissue processor In-Reply-To: <650420.47113.qm@web44808.mail.sp1.yahoo.com> References: <650420.47113.qm@web44808.mail.sp1.yahoo.com> Message-ID: <625858D937841B4D89752F7B6C35984901FA18B2@romba.vsl.com.au> Dr. Evette Demaisip, Thank you for your posting. We have forwarded this to your Leica representative for the Philippines. They should be in contact with you shortly. Alternatively in future you may also contact histosupport@leica-microsystems.com for further assistance. Best Regards, Stella Argyriou Global Product Manager Biosystems Division Leica Biosystems Melbourne Pty Ltd 495 Blackburn Road Mt Waverley VIC 3149 Australia Telephone +61 3 9211 7514 Facsimile +61 3 9211 7401 www.leica-microsystems.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of evette demaisip Sent: Thursday, 10 September 2009 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols.?We encountered problems in the?8-hr?and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome.??My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for?feedback/advice on the ff;? 1. Possible reasons? 2.?Steps to prevent this in the future? 3. Reprocessing protocol in Peloris. Thank you so much. Dr. Evette Demaisip Pathologist,Philippines Get your new Email address! Grab the Email name you've always wanted before someone else does! http://mail.promotions.yahoo.com/newdomains/aa/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Sep 17 07:08:59 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Sep 17 07:09:05 2009 Subject: [Histonet] air quality in the lab Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07A1FB72@wahtntex2.waht.swest.nhs.uk> That's how often you have to turnover the water in a Koi Pond; 4 times and hour. Do you keep fish? Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 16 September 2009 16:53 To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu; Merced M Leiker Subject: Re: [Histonet] air quality in the lab From?the recesses of my sometimes foggy memory, the figure of 4 exchanges/hour has popped up. Perhaps somebody else has some other figure. Ren? J. --- On Wed, 9/16/09, Merced M Leiker wrote: From: Merced M Leiker Subject: Re: [Histonet] air quality in the lab To: godsgalnow@aol.com, histonet@lists.utsouthwestern.edu Date: Wednesday, September 16, 2009, 11:11 AM that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgalnow@aol.com wrote: > > Does anyone know what the regulations or requirements? are for the air > turnover in the lab? > > > >? Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Thu Sep 17 07:09:46 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Sep 17 07:10:15 2009 Subject: [Histonet] mib-1 Message-ID: "Alcohol based" suggests to me an absence of formalin. In which case, epitope retreival is not indicated. Also, you didn't say if it had been working and stopped or if this was a new marker for your lab. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> 9/16/2009 3:44 PM >>> I have to turn to the experts here.? I am having problems with the MIB-1.? I am having an issue with the patient samples working.? We use an alcohol based fixative....it has always worked until recently. I have tried everything I can think of to no avail.? I have decreased the titer, increased the incubation, tried a different retrieval solution, tried a different detection...nothing is working. Anyone else having any issues? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From godsgalnow <@t> aol.com Thu Sep 17 08:36:49 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Sep 17 08:37:23 2009 Subject: [Histonet] mib-1 In-Reply-To: References: Message-ID: <8CC05B30B3D8209-42A8-7016@webmail-m081.sysops.aol.com> Correct....the formalin is neglegable.? We have been using this antobody for years with minor issues.? Over the past few weeks we are running into a problem with false negatives.? The control is working fine. -----Original Message----- From: Greg Dobbin To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Sent: Thu, Sep 17, 2009 8:09 am Subject: Re: [Histonet] mib-1 "Alcohol based" suggests to me an absence of formalin. In which case, epitope retreival is not indicated. Also, you didn't say if it had been working and stopped or if this was a new marker for your lab. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> 9/16/2009 3:44 PM >>> I have to turn to the experts here.? I am having problems with the MIB-1.? I am having an issue with the patient samples working.? We use an alcohol based fixative....it has always worked until recently. I have tried everything I can think of to no avail.? I have decreased the titer, increased the incubation, tried a different retrieval solution, tried a different detection...nothing is working. Anyone else having any issues? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From badzrosari <@t> yahoo.com Thu Sep 17 08:57:49 2009 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Thu Sep 17 08:57:53 2009 Subject: [Histonet] (no subject) Message-ID: <307385.15665.qm@web52108.mail.re2.yahoo.com> HI HISTONETS...IM USUALLY SENDING FEW OF MY SAMPLES FOR IHC OUTSIDE THE LAB...IS THERE SOMEONE THERE INTERESTED TO ACCEPT AN ENDOMETRIAL BIOPSY FOR NK CELL STUDY???PATIENT IS VERY SPECIFIC EVEN SIMILAR TEST ALREADY DONE LIKE CD8,CD20,CD3,CD16,CD56....ANYBODY?? From Nancy.Herman <@t> inspection.gc.ca Thu Sep 17 09:12:36 2009 From: Nancy.Herman <@t> inspection.gc.ca (Nancy Herman) Date: Thu Sep 17 09:12:47 2009 Subject: [Histonet] DAB Chromogen Message-ID: <4AB1EF74020000DD00001A83@inspection.gc.ca> I have this DAB staining protocol for IHC. It was used on the DAKO autostainer and incubated for 5min. Our controls using the DAKO DAB chromogen kit worked fine. Does anyone have any experience with a DAB protocol similar to this or have any other information to add to this protocol? Is incubating on ice necessary? TRIS-DAB: 200 ml TRIS-DAB, 0.05M, Ph 7.6 40 mg DAB (aliquots in 1.5 ml, -20 Celsius) filter incubate slides (eventually on ice) add: 34 ul H2O2 (30%) stop reaction in PBS check slides under the microscope Thanks, Nancy Animal Disease Research Laboratory Canada From Nancy.Herman <@t> inspection.gc.ca Thu Sep 17 09:45:53 2009 From: Nancy.Herman <@t> inspection.gc.ca (Nancy Herman) Date: Thu Sep 17 09:46:03 2009 Subject: [Histonet] DAB Chromogen In-Reply-To: <4AB1EF74020000DD00001A83@inspection.gc.ca> References: <4AB1EF74020000DD00001A83@inspection.gc.ca> Message-ID: <4AB1F741020000DD00001A87@inspection.gc.ca> Sorry, forgot to mention we had no staining results for the Tris-DAB protocol. >>> "Nancy Herman" 2009/09/17 8:12 am >>> I have this DAB staining protocol for IHC. It was used on the DAKO autostainer and incubated for 5min. Our controls using the DAKO DAB chromogen kit worked fine. Does anyone have any experience with a DAB protocol similar to this or have any other information to add to this protocol? Is incubating on ice necessary? TRIS-DAB: 200 ml TRIS-DAB, 0.05M, Ph 7.6 40 mg DAB (aliquots in 1.5 ml, -20 Celsius) filter incubate slides (eventually on ice) add: 34 ul H2O2 (30%) stop reaction in PBS check slides under the microscope Thanks, Nancy Animal Disease Research Laboratory Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nefff <@t> staff.uni-marburg.de Thu Sep 17 09:52:24 2009 From: nefff <@t> staff.uni-marburg.de (Dr. Frauke Neff) Date: Thu Sep 17 09:52:36 2009 Subject: [Histonet] indian ink Message-ID: <20090917165224.7w6onyr9o0o8ok8k@home.staff.uni-marburg.de> Dear everyone, when I was working in pathology gross section lab, we used black dye (and blue, green etc,"Tusche" in german) for orientation of the gross section specimens. We fixed the dye with Bouin's solution. Now I was asked for this dye, but I don't know if this was the "conventional" india ink you can buy for drawing in paper shops or was this a "special dye". I don't found anything in commen pathology supplier catalogs and can't reach anyone of my former colleagues who might know. Can you help me out of this confusion?! Thanks a lot (Vielen Dank) Frauke From peter.craven <@t> nhs.net Thu Sep 17 09:58:38 2009 From: peter.craven <@t> nhs.net (Craven Peter (NHS Highland)) Date: Thu Sep 17 10:02:50 2009 Subject: [Histonet] indian ink In-Reply-To: <20090917165224.7w6onyr9o0o8ok8k@home.staff.uni-marburg.de> References: <20090917165224.7w6onyr9o0o8ok8k@home.staff.uni-marburg.de> Message-ID: <4FF435FF1198AB43ABA6976904E69C2962B4000ABF@NHS-PCLI-MBC001.AD1.NHS.NET> Frauke In the United Kingdom Cellpath and Thermo Shandon both sell tissue marking dyes in a range of differing colours we have used both versions with no problems for marking resection margins prior to processing. Hope this helps Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital Old Perth Road Inverness IV2 3UJ Tel 01463 704269 email peter.craven@nhs.net ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. Frauke Neff [nefff@staff.uni-marburg.de] Sent: 17 September 2009 03:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] indian ink Dear everyone, when I was working in pathology gross section lab, we used black dye (and blue, green etc,"Tusche" in german) for orientation of the gross section specimens. We fixed the dye with Bouin's solution. Now I was asked for this dye, but I don't know if this was the "conventional" india ink you can buy for drawing in paper shops or was this a "special dye". I don't found anything in commen pathology supplier catalogs and can't reach anyone of my former colleagues who might know. Can you help me out of this confusion?! Thanks a lot (Vielen Dank) Frauke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSI recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere For more information and to find out how you can switch, visit www.connectingforhealth.nhs.uk/nhsmail ******************************************************************************************************************** From rjbuesa <@t> yahoo.com Thu Sep 17 10:03:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 17 10:03:51 2009 Subject: [Histonet] indian ink In-Reply-To: <20090917165224.7w6onyr9o0o8ok8k@home.staff.uni-marburg.de> Message-ID: <470552.3253.qm@web65711.mail.ac4.yahoo.com> They are talking about the?common Indian ink, nothing else and you will find it, as you write, in a office supplies store. Ren? J. --- On Thu, 9/17/09, Dr. Frauke Neff wrote: From: Dr. Frauke Neff Subject: [Histonet] indian ink To: histonet@lists.utsouthwestern.edu Date: Thursday, September 17, 2009, 10:52 AM Dear everyone, when I was working in pathology gross section lab, we used black dye (and blue, green etc,"Tusche" in german) for orientation of the gross section specimens. We fixed the dye with Bouin's solution. Now I was asked for this dye, but I don't know if this was the "conventional" india ink you can buy for drawing in paper shops or was this a "special dye". I don't found anything in commen pathology supplier catalogs and can't reach anyone of my former colleagues who might know. Can you help me out of this confusion?! Thanks a lot (Vielen Dank) Frauke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Sep 17 10:06:53 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Sep 17 10:07:07 2009 Subject: [Histonet] indian ink In-Reply-To: <470552.3253.qm@web65711.mail.ac4.yahoo.com> References: <20090917165224.7w6onyr9o0o8ok8k@home.staff.uni-marburg.de> <470552.3253.qm@web65711.mail.ac4.yahoo.com> Message-ID: <003401ca37a8$7e08cda0$7a1a68e0$@net> Be sure you get waterproof india ink as they now sell two kinds. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 17, 2009 11:04 AM To: histonet@lists.utsouthwestern.edu; Dr. Frauke Neff Subject: Re: [Histonet] indian ink They are talking about the?common Indian ink, nothing else and you will find it, as you write, in a office supplies store. Ren? J. --- On Thu, 9/17/09, Dr. Frauke Neff wrote: From: Dr. Frauke Neff Subject: [Histonet] indian ink To: histonet@lists.utsouthwestern.edu Date: Thursday, September 17, 2009, 10:52 AM Dear everyone, when I was working in pathology gross section lab, we used black dye (and blue, green etc,"Tusche" in german) for orientation of the gross section specimens. We fixed the dye with Bouin's solution. Now I was asked for this dye, but I don't know if this was the "conventional" india ink you can buy for drawing in paper shops or was this a "special dye". I don't found anything in commen pathology supplier catalogs and can't reach anyone of my former colleagues who might know. Can you help me out of this confusion?! Thanks a lot (Vielen Dank) Frauke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Sep 17 10:08:21 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 17 10:09:24 2009 Subject: [Histonet] indian ink In-Reply-To: <470552.3253.qm@web65711.mail.ac4.yahoo.com> References: <20090917165224.7w6onyr9o0o8ok8k@home.staff.uni-marburg.de> <470552.3253.qm@web65711.mail.ac4.yahoo.com> Message-ID: And Higgins brand is the best to survive the solvents. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 17, 2009 11:04 To: histonet@lists.utsouthwestern.edu; Dr. Frauke Neff Subject: Re: [Histonet] indian ink They are talking about the?common Indian ink, nothing else and you will find it, as you write, in a office supplies store. Ren? J. --- On Thu, 9/17/09, Dr. Frauke Neff wrote: From: Dr. Frauke Neff Subject: [Histonet] indian ink To: histonet@lists.utsouthwestern.edu Date: Thursday, September 17, 2009, 10:52 AM Dear everyone, when I was working in pathology gross section lab, we used black dye (and blue, green etc,"Tusche" in german) for orientation of the gross section specimens. We fixed the dye with Bouin's solution. Now I was asked for this dye, but I don't know if this was the "conventional" india ink you can buy for drawing in paper shops or was this a "special dye". I don't found anything in commen pathology supplier catalogs and can't reach anyone of my former colleagues who might know. Can you help me out of this confusion?! Thanks a lot (Vielen Dank) Frauke _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From zhang156 <@t> purdue.edu Thu Sep 17 10:10:49 2009 From: zhang156 <@t> purdue.edu (Yuqing Zhang) Date: Thu Sep 17 10:10:55 2009 Subject: [Histonet] Please help me with my cryosections Message-ID: <1253200249.4ab25179605e3@webmail.purdue.edu> Hi everybody! A friend recommended this forum to me as he said lots of experts here could help me with my horrible cryosections. Right now, I am working on a gene expression pattern in zebrafish retina. I need to section the eye before I see the expression clearly. So I do the whole mount in situ hybridization first, then embed the embryos in TFM (some thing like OCT) for cryosections. But I have several problems with my sections. 1. Some sections have big holes inside, but the cell shape is still fine , this kind of situation happens more in younger embryos (36 hour post fertilization) 2. In some sections, the cells seemed became one big block, and there are some strange patches inside the section, cells seemed aggregated to each other 3. Some sections have small holes, the cell shape is totally indistinguishable. I have tried several approaches to find out the reason, but non of them worked. 1. Slides of different brand. 2. gradient sucrose concentration (10%, 20%, 30%). or only 15% sucrose. 3. Do not fix embryos in PFA after in situ, keep them in PBST. 4. Freeze sample in liquid nitrogen. Or freeze them in cryostat. I wanted to attach the pictures and protocol I am using, but the system said it it too big to be posted. Thanks a lot in advance for you help. Yuqing Zhang From rjbuesa <@t> yahoo.com Thu Sep 17 09:59:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 17 10:14:52 2009 Subject: [Histonet] DAB Chromogen In-Reply-To: <4AB1F741020000DD00001A87@inspection.gc.ca> Message-ID: <708912.58171.qm@web65713.mail.ac4.yahoo.com> Dilute 6 mg of DAB in 15 mL of PBS buffer pH7?and add 200 ?L of 3% hydrogen peroxide just before using and incubate at room temp. Ren? J. --- On Thu, 9/17/09, Nancy Herman wrote: From: Nancy Herman Subject: Re: [Histonet] DAB Chromogen To: "Nancy Herman" , histonet@lists.utsouthwestern.edu Date: Thursday, September 17, 2009, 10:45 AM Sorry, forgot to mention we had no staining results for the Tris-DAB protocol. >>> "Nancy Herman" 2009/09/17 8:12 am >>> I have this DAB staining protocol for IHC.? It was used on the DAKO autostainer and incubated for 5min.? Our controls using the DAKO DAB chromogen kit worked fine.? Does anyone have any experience with a DAB protocol similar to this or have any other information to add to this protocol?? Is incubating on ice necessary? TRIS-DAB: 200 ml TRIS-DAB, 0.05M, Ph 7.6 40 mg DAB (aliquots in 1.5 ml, -20 Celsius) filter incubate slides (eventually on ice) add: 34 ul H2O2 (30%) stop reaction in PBS check slides under the microscope Thanks, Nancy Animal Disease Research Laboratory Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 17 09:50:56 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 17 10:15:04 2009 Subject: [Histonet] DAB Chromogen In-Reply-To: <4AB1EF74020000DD00001A83@inspection.gc.ca> Message-ID: <571851.59841.qm@web65704.mail.ac4.yahoo.com> That seems to be a protocol for a very specific situation but incubation over ice is absolutely not necessary and if done like that the incubation time has to be prolonged substantially. An incubation tie of 5 min at room temperature (as in the DAKO autostainer) is all that is required. Ren? J. --- On Thu, 9/17/09, Nancy Herman wrote: From: Nancy Herman Subject: [Histonet] DAB Chromogen To: histonet@lists.utsouthwestern.edu Date: Thursday, September 17, 2009, 10:12 AM I have this DAB staining protocol for IHC.? It was used on the DAKO autostainer and incubated for 5min.? Our controls using the DAKO DAB chromogen kit worked fine.? Does anyone have any experience with a DAB protocol similar to this or have any other information to add to this protocol?? Is incubating on ice necessary? TRIS-DAB: 200 ml TRIS-DAB, 0.05M, Ph 7.6 40 mg DAB (aliquots in 1.5 ml, -20 Celsius) filter incubate slides (eventually on ice) add: 34 ul H2O2 (30%) stop reaction in PBS check slides under the microscope Thanks, Nancy Animal Disease Research Laboratory Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asachau <@t> aureusmedical.com Thu Sep 17 10:19:25 2009 From: asachau <@t> aureusmedical.com (asachau@aureusmedical.com) Date: Thu Sep 17 10:19:30 2009 Subject: [Histonet] Submissions Message-ID: I haven't been receiving any emails??? April Sachau, Account Manager Laboratory Division C&A Plaza Omaha (402) 891-1118 x6047 (800) 856-0741 asachau@aureusmedical.com www.aur Ref#[14586571] This communication, along with any attachments, is covered by federal and s confidential a this message is not th that any dissemination, distr is strictly prohibited. If you hav reply immediately to the sender and delete From relia1 <@t> earthlink.net Thu Sep 17 10:27:57 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Sep 17 10:28:02 2009 Subject: [Histonet] RELIA Special Job Alert Research Investigator (PhD) needed for biotech firm in San Francisco Bay Area! Message-ID: Hi Histonetters! I hope everyone is having a great day. I have a new opportunity that I wanted to tell everyone about. I am currently working with a prestigious biotech firm located in the San Francisco Bay area that is in need of a Research Investigator. They are looking for someone with a PhD, strong research and investigative experience and strong histology skills. If you or someone you know might be interested in hearing more about the opportunity please contact me. I can be reached by e-mail at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From suewalls123 <@t> live.com Thu Sep 17 10:38:25 2009 From: suewalls123 <@t> live.com (Sue Walls) Date: Thu Sep 17 10:38:31 2009 Subject: [Histonet] TISSUE FREEZING MEDIUM In-Reply-To: <757965.2400.qm@web1115.biz.mail.sk1.yahoo.com> References: <757965.2400.qm@web1115.biz.mail.sk1.yahoo.com> Message-ID: Carol, I use OCT from a company US Laboratory Supplies It seem to do the job, no residue, light refractive. I have tried to switch to lower cost OCT, such as Tissue Tek, but I think some companies have been cutting corners in manufacturing. Sue ------------------------------ Message: 2 Date: Wed, 16 Sep 2009 13:30:28 -0400 From: "Carol Buckingham" Subject: [Histonet] TISSUE FREEZING MEDIUM To: Message-ID: <4AB0E8740200007E0001361F@email.chop.edu> Content-Type: text/plain; charset=US-ASCII I'm having a great deal of trouble finding a tissue freezing medium that is anything like the original OCT -15-25. Since OCT is no longer available at different temperature uses, I cannot cut a good section in our cryostat(-25)without using freezing spray. Even after trying a few different brands of medium,without freezing spray, our sections are still folded,wavy,and all around not of good quality like we used to have. Most of the different brands are fine for a specimen that was just snap frozen and cut, as in a surgical frozen case, but not good for serial sectioning that may take place 1 to 2 days later, as in a renal bx.We have tried to cut on three different cryostats and had similar results.Surely there are those reading this that are having the same problem.Thanks in advance for your help. _________________________________________________________________ Bing? brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1 From esulkosky <@t> gmail.com Thu Sep 17 10:41:49 2009 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Thu Sep 17 10:41:53 2009 Subject: [Histonet] TISSUE FREEZING MEDIUM Message-ID: <6c3840890909170841u2bee61e7qab43939751bef1d0@mail.gmail.com> Carol, I have heard of similar problems. I believe the temperature of your cryostat may be one of the reasons for your problem along with the type of media. Optimal cryostat temps for cutting unfixed tissue such as brain, lymph node, liver and kidney should be -12- 16 degrees. Fatty tissue such as breast and skin, thyroid, prostate and adrenal should be at -18- 30 degrees. Have you tried cutting at a different temperature? No sure if it will resolve your problem completely but it should improve your results. Hope this helps. Eric From Maria.Katleba <@t> stjoe.org Thu Sep 17 11:33:11 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Sep 17 11:33:23 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> References: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> Message-ID: <97C02552ECB11346877D3E83CF833ABD13ED6547E7@SJSNT-SCMAIL03.stjoe.org> Roxanne, I got this question on the Histonet Blog... Since this is what you do for a living, I was wondering if you could help us out here. You can reply to ALL. Maria Katleba Queen of the Valley Medical Center Napa CA 707-257-4076 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Wednesday, September 16, 2009 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air quality in the lab Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From godsgalnow <@t> aol.com Thu Sep 17 12:00:38 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Sep 17 12:00:56 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13ED6547E7@SJSNT-SCMAIL03.stjoe.org> References: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> <97C02552ECB11346877D3E83CF833ABD13ED6547E7@SJSNT-SCMAIL03.stjoe.org> Message-ID: <8CC05CF842543CD-2C40-13C4D@webmail-m020.sysops.aol.com> Maria, Thanks for the compliment...but unfortunately even I do not know everything....... It was I that sent the original email. Roxanne -----Original Message----- From: Maria Katleba To: godsgalnow@aol.com ; histonet@lists.utsouthwestern.edu Cc: Roxanne Fynboh Sent: Thu, Sep 17, 2009 12:33 pm Subject: RE: [Histonet] air quality in the lab Roxanne, I got this question on the Histonet Blog... Since this is what you do for a living, I was wondering if you could help us out here. You can reply to ALL. Maria Katleba Queen of the Valley Medical Center Napa CA 707-257-4076 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Wednesday, September 16, 2009 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air quality in the lab Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From gayle.callis <@t> bresnan.net Thu Sep 17 12:43:36 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Sep 17 12:43:58 2009 Subject: [Histonet] RE: Tissue Freezing Medium Message-ID: <000401ca37be$6393e070$2abba150$@callis@bresnan.net> One has to be careful in calling ALL cryoembedding mediums OCT since this is a trademarked product from Sakura Finetek, under the name Tissue Tek OCT. If you use other than OCT, then you need to give the name and specify WHICH brand you are using in order to lessen confusion. Out of curiosity and always shopping for a new product, we have tried as many cryoembedding medias possible and found them to be different from each other e.g. none of them are exactly the same nor do they work for all applications as in research where one may fill an intestine or lung with a cryoembedding media. The differences are the in the ingredients, what molecular weights may be involved for the polyethylene glycol or polyvinyl alcohols used or other ingredients that may be different. Be sure to look at the label and MSDS (hopefully tell the chemicals used!) and if you know which one you had success with to start with, I suggest going back to that media. The original Tissue Tek OCT has the ingredients written on the bottle, but some information, the molecular weights of the PVA and PEG is proprietary. - the only information is percentage of the ingredients listed. As a side comment, I was fortunate to have a discussion about OCT with Dr. McCormick who was involved with develop OCT for Miles/Tissue Tek aka Sakura. He said he could make up different mixtures for use at whatever cryosectioning temperature was desired. After trying a new (and graciously supplied by vendor) cryoembedding media that did not work for our research application, I learned via the company tech services the product had only been tested on human tissues, and never with research animal tissues. Consequently, we continue to use Tissue Tek OCT and at any temperature we desire, from - 16C to -35C and at section thicknesses from 2 um up to 50 um or more. It also worked perfectly for our human renal biopsy service for over twelve years. The one message changing temperature according to tissue type is good advice. However, in a clinical diagnostic setting and under time constraints, this could be difficult unless a cryostat has two compressors to speed up specimen temperature change. There are other ways to cool a sample - one suggestion from years ago was an awkward setup to let liquid nitrogen fumes falling down and over the sample to cool it before sectioning although some techs now spray Liq N2 on the sample. Cryosprays are not something I care to breathe however. Also make sure your blade holders, etc are tight, well adjusted - someone may have inadvertently changed settings. Or are you working with new brand of disposable blades - another parameter to consider? Good luck on solving the problem Gayle M. Callis HTL/HT/MT(ASCP) From eridana <@t> cox.net Thu Sep 17 12:49:29 2009 From: eridana <@t> cox.net (Eridana) Date: Thu Sep 17 12:49:34 2009 Subject: [Histonet] Polarizing filters In-Reply-To: <20090917151733.XFPK15301.eastrmmtai102.cox.net@eastrmimpi01.cox.net> Message-ID: <20090917134929.HKESP.239930.imail@fed1rmwml29> You can use glass camera filters. At the store they had a huge supply of polarizing filters. I bought 2 for about $20 each that were the same brand, but not even the same diameter. I put one on top of the slide and one on the light source and it worked great. I also rotated the light source filter since it was too easy to bump the slide when trying to rotate the upper one. It was really interesting to see all the non collagen that was positive in the staining but not when polarized. Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2010 San Diego, CA 92071 858 534 7438 Date: Wed, 16 Sep 2009 14:12:38 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] Polarizing filters To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CE6@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" The polarizer and analyzer are identical filters, and either of them can be used in either location. One must be between the light source and the slide being viewed. The other must be between the slide being viewed and your eye or camera. I place one filter directly on top of my illuminator. The other is in a filter slide in the microscope column, which can be pushed into the light beam or pulled out of it, but you can also place it directly on top of the slide. You rotate either filter to achieve the polarization effect. I rotate the lower one since the other one is not accessible. These filters cut down the light intensity substantially, so you should use them with maximum brightness of the illuminator, iris diaphram wide open, and with neutral density or any other kinds of filters removed from the light beam, including the blue filter if you normally use one. Polarizing filters can be purchased at any camera store, and some science supply companies sell them. Get good quality glass filters though, not cheap plastic ones. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk > Sent: Thursday, September 10, 2009 4:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Polarizing filters > > Does anyone know where I can find the two appropriate filters (lenses) > needed to polarize the congo red and Sirius red stains? I have an Olympus > CH-2 that needs to be fitted. I understand I need a "polarizer" lens and an > "analyzer" lens. Are these two different lenses or the same lens, just in > different locations on the microscope? > > Thank you > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > From leiker <@t> buffalo.edu Thu Sep 17 13:44:59 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Sep 17 13:45:07 2009 Subject: [Histonet] air quality in the lab In-Reply-To: <8CC05CF842543CD-2C40-13C4D@webmail-m020.sysops.aol.com> References: <8CC04F2C3E5DBC4-2780-694@webmail-d047.sysops.aol.com> <97C02552ECB11346877D3E83CF833ABD13ED6547E7@SJSNT-SCMAIL03.stjoe.org> <8CC05CF842543CD-2C40-13C4D@webmail-m020.sysops.aol.com> Message-ID: LOL that is good for a Thursday. But Friday's tomorrow. ;-) --On Thursday, September 17, 2009 1:00 PM -0400 godsgalnow@aol.com wrote: > > Maria, > > > > Thanks for the compliment...but unfortunately even I do not know > everything....... > > > > It was I that sent the original email. > > > > Roxanne > > > -----Original Message----- > From: Maria Katleba > To: godsgalnow@aol.com ; > histonet@lists.utsouthwestern.edu Cc: > Roxanne Fynboh > Sent: Thu, Sep 17, 2009 12:33 pm > Subject: RE: [Histonet] air quality in the lab > > > > > Roxanne, > > I got this question on the Histonet Blog... Since this is what you do for > a living, I was wondering if you could help us out here. > > You can reply to ALL. > > Maria Katleba > Queen of the Valley Medical Center > Napa CA > 707-257-4076 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > godsgalnow@aol.com > Sent: Wednesday, September 16, 2009 7:40 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] air quality in the lab > > > Does anyone know what the regulations or requirements are for the air > turnover in the lab? > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Notice from St. Joseph Health System: > Please note that the information contained in this message may be > privileged and confidential and protected from disclosure. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Tony_Reilly <@t> health.qld.gov.au Thu Sep 17 18:49:15 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Sep 17 18:50:42 2009 Subject: [Histonet] Polarizing filters In-Reply-To: <20090917134929.HKESP.239930.imail@fed1rmwml29> References: <20090917151733.XFPK15301.eastrmmtai102.cox.net@eastrmimpi01.cox.net> <20090917134929.HKESP.239930.imail@fed1rmwml29> Message-ID: <4AB3579B.471C.0039.0@health.qld.gov.au> I worked with a pathologist many years ago who would use one piece of polarising glass on the light source and wear his sunglasses. It worked fine. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> Eridana 18/09/2009 3:49 am >>> You can use glass camera filters. At the store they had a huge supply of polarizing filters. I bought 2 for about $20 each that were the same brand, but not even the same diameter. I put one on top of the slide and one on the light source and it worked great. I also rotated the light source filter since it was too easy to bump the slide when trying to rotate the upper one. It was really interesting to see all the non collagen that was positive in the staining but not when polarized. Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2010 San Diego, CA 92071 858 534 7438 Date: Wed, 16 Sep 2009 14:12:38 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] Polarizing filters To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CE6@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain;charset="iso-8859-1" The polarizer and analyzer are identical filters, and either of them can be used in either location. One must be between the light source and the slide being viewed. The other must be between the slide being viewed and your eye or camera. I place one filter directly on top of my illuminator. The other is in a filter slide in the microscope column, which can be pushed into the light beam or pulled out of it, but you can also place it directly on top of the slide. You rotate either filter to achieve the polarization effect. I rotate the lower one since the other one is not accessible. These filters cut down the light intensity substantially, so you should use them with maximum brightness of the illuminator, iris diaphram wide open, and with neutral density or any other kinds of filters removed from the light beam, including the blue filter if you normally use one. Polarizing filters can be purchased at any camera store, and some science supply companies sell them. Get good quality glass filters though, not cheap plastic ones. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk > Sent: Thursday, September 10, 2009 4:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Polarizing filters > > Does anyone know where I can find the two appropriate filters (lenses) > needed to polarize the congo red and Sirius red stains? I have an Olympus > CH-2 that needs to be fitted. I understand I need a "polarizer" lens and an > "analyzer" lens. Are these two different lenses or the same lens, just in > different locations on the microscope? > > Thank you > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From lucie.s.guernsey <@t> gmail.com Thu Sep 17 20:15:15 2009 From: lucie.s.guernsey <@t> gmail.com (Lucie Guernsey) Date: Thu Sep 17 20:48:45 2009 Subject: [Histonet] Ultra-Low Temp. Upright Freezer Message-ID: Hi all! I'm hoping to get some help RE: purchasing a new ultra-low temp. upright freezer. I've tried searching Histonet, but many of the posts are a few years old, and my guess is that the products have changed a bit over the years. My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., but preferably much larger), and definitely upright (not chest) -80. Unfortunately, since this is my first foray into the world of lab freezers, I know very little about them. I have discovered that Thermo's Revco and Sanyo freezers seem to be popular. Obviously we're looking for a freezer that is as large and efficient as possible. Insulation and the freezer's compressor(s) are most important. I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) freezer seems quite nice - small exterior footprint and his sales pitch regarding the vacuum insulation (therefore denser insulation), insulated inner doors, and supposed minimized carbon footprint was quite convincing. I still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, though my guess is that their sales pitch will be just as convincing. Since you can't depend only on sales pitches, I'm depending on colleagues as well.... Does anyone have any experience with either the Sanyo or Revco freezers (or any others that they'd like to recommend)? Any and all positive and negative feedback would be greatly appreciated! I'm hoping to get comments regarding: - efficiency - how often service is needed due to failures - are the alarms sensitive enough or maybe too sensitive - how loud the freezer is when the compressor is on - etc...... As always, please remember to Reply All. Thank you in advance for your help! I look forward to hearing everyone's suggestions. Lucie UCSD lguernsey@ucsd.edu From dennijc <@t> vetmed.auburn.edu Fri Sep 18 07:53:21 2009 From: dennijc <@t> vetmed.auburn.edu (John C. Dennis) Date: Fri Sep 18 07:53:29 2009 Subject: [Histonet] Ultra-Low Temp. Upright Freezer In-Reply-To: References: Message-ID: Dear Lucie We're buying a new -80 freezer soon. The word I got from those with experience is: So-Low is not so good. REVCO (the we are replacing) once was good but has declined in quality (along with GM et al, apparently) Forma is the one we're buying. NOTE: the high end model requires 220 V. Ours is the smaller (23 cu ft) 110 V model. John Carroll Dennis Anatomy, Physiology, and Pharmacology 109 Greene Hall Auburn University, AL 36849 On Thu, 17 Sep 2009, Lucie Guernsey wrote: > Hi all! > > I'm hoping to get some help RE: purchasing a new ultra-low temp. upright > freezer. I've tried searching Histonet, but many of the posts are a few > years old, and my guess is that the products have changed a bit over the > years. > > My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., > but preferably much larger), and definitely upright (not chest) -80. > Unfortunately, since this is my first foray into the world of lab freezers, > I know very little about them. I have discovered that Thermo's Revco and > Sanyo freezers seem to be popular. Obviously we're looking for a freezer > that is as large and efficient as possible. Insulation and the freezer's > compressor(s) are most important. > > I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) > freezer seems quite nice - small exterior footprint and his sales pitch > regarding the vacuum insulation (therefore denser insulation), insulated > inner doors, and supposed minimized carbon footprint was quite convincing. I > still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, > though my guess is that their sales pitch will be just as convincing. > > Since you can't depend only on sales pitches, I'm depending on colleagues as > well.... Does anyone have any experience with either the Sanyo or Revco > freezers (or any others that they'd like to recommend)? Any and all positive > and negative feedback would be greatly appreciated! I'm hoping to get > comments regarding: > - efficiency > - how often service is needed due to failures > - are the alarms sensitive enough or maybe too sensitive > - how loud the freezer is when the compressor is on > - etc...... > > As always, please remember to Reply All. > > Thank you in advance for your help! I look forward to hearing everyone's > suggestions. > > > Lucie > > UCSD > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Sep 18 08:08:12 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 18 08:08:20 2009 Subject: [Histonet] Ultra-Low Temp. Upright Freezer In-Reply-To: Message-ID: <879339.97240.qm@web65713.mail.ac4.yahoo.com> I always used Revco freezers with any problems. Ren? J. --- On Thu, 9/17/09, Lucie Guernsey wrote: From: Lucie Guernsey Subject: [Histonet] Ultra-Low Temp. Upright Freezer To: histonet@lists.utsouthwestern.edu Date: Thursday, September 17, 2009, 9:15 PM Hi all! I'm hoping to get some help RE: purchasing a new ultra-low temp. upright freezer. I've tried searching Histonet, but many of the posts are a few years old, and my guess is that the products have changed a bit over the years. My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., but preferably much larger), and definitely upright (not chest) -80. Unfortunately, since this is my first foray into the world of lab freezers, I know very little about them. I have discovered that Thermo's Revco and Sanyo freezers seem to be popular. Obviously we're looking for a freezer that is as large and efficient as possible. Insulation and the freezer's compressor(s) are most important. I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) freezer seems quite nice - small exterior footprint and his sales pitch regarding the vacuum insulation (therefore denser insulation), insulated inner doors, and supposed minimized carbon footprint was quite convincing. I still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, though my guess is that their sales pitch will be just as convincing. Since you can't depend only on sales pitches, I'm depending on colleagues as well.... Does anyone have any experience with either the Sanyo or Revco freezers (or any others that they'd like to recommend)? Any and all positive and negative feedback would be greatly appreciated! I'm hoping to get comments regarding: ? ? - efficiency ? ? - how often service is needed due to failures ? ? - are the alarms sensitive enough or maybe too sensitive ? ? - how loud the freezer is when the compressor is on ? ? - etc...... As always, please remember to Reply All. Thank you in advance for your help! I look forward to hearing everyone's suggestions. Lucie UCSD lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dreynold <@t> mdanderson.org Fri Sep 18 08:19:36 2009 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Fri Sep 18 08:19:43 2009 Subject: [Histonet] IHCCRG; RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F021613010D6A@DCPWVMBXC0VS3.mdanderson.edu> References: <37212590E341574B96E6796D27DF42DAB04B7D@RWDEX3.WHS.phci.org> <785BBF0C5F49CE41BA74460A43A08F021613010D6A@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F021613010D6C@DCPWVMBXC0VS3.mdanderson.edu> From: Reynolds,Donna M Sent: Friday, September 18, 2009 8:01 AM To: 'ihcrg@googlegroups.com' Subject: RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens I have attached a decal procedure we have used for a long time. We use it for mouse bones mostly. But we have also used it for Human bone marrow bx. We have beautiful IHC's using this method. Donna Reynolds HT(ASCP) Chief Histology Laboratory Dept. Cancer Biology U.T. M.D. Anderson Cancer Center Houston, Texas 713-792-8106 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Tuesday, September 15, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [IHCRG] Use of immuno cal or other gentle formic acid decal solution for immuno specimens Hi all, Would someone share with me their times and procedure for formic acid decal of bone marrows for IHC. Thanks Deb Deb Van Eyck CT(ASCP) QIHC Waukesha Memorial Hospital 725 American Avenue Waukesha,WI 53188 262-928-2112 This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From katherine-walters <@t> uiowa.edu Fri Sep 18 08:24:25 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Sep 18 08:24:30 2009 Subject: [Histonet] IHCCRG; RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F021613010D6C@DCPWVMBXC0VS3.mdanderson.edu> References: <37212590E341574B96E6796D27DF42DAB04B7D@RWDEX3.WHS.phci.org><785BBF0C5F49CE41BA74460A43A08F021613010D6A@DCPWVMBXC0VS3.mdanderson.edu> <785BBF0C5F49CE41BA74460A43A08F021613010D6C@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: Would you please post this procedure? Thanks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Friday, September 18, 2009 8:20 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHCCRG; RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens From: Reynolds,Donna M Sent: Friday, September 18, 2009 8:01 AM To: 'ihcrg@googlegroups.com' Subject: RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens I have attached a decal procedure we have used for a long time. We use it for mouse bones mostly. But we have also used it for Human bone marrow bx. We have beautiful IHC's using this method. Donna Reynolds HT(ASCP) Chief Histology Laboratory Dept. Cancer Biology U.T. M.D. Anderson Cancer Center Houston, Texas 713-792-8106 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Tuesday, September 15, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [IHCRG] Use of immuno cal or other gentle formic acid decal solution for immuno specimens Hi all, Would someone share with me their times and procedure for formic acid decal of bone marrows for IHC. Thanks Deb Deb Van Eyck CT(ASCP) QIHC Waukesha Memorial Hospital 725 American Avenue Waukesha,WI 53188 262-928-2112 This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From julia.m.hough <@t> hotmail.co.uk Fri Sep 18 09:10:22 2009 From: julia.m.hough <@t> hotmail.co.uk (julia hough) Date: Fri Sep 18 09:10:27 2009 Subject: [Histonet] zinc fixation Message-ID: Hello. Histonetters. I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also. I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were ?jelly-like? before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. Thanks Julia _________________________________________________________________ Learn how to add other email accounts to Hotmail in 3 easy steps. http://clk.atdmt.com/UKM/go/167688463/direct/01/ From j.hough <@t> sheffield.ac.uk Fri Sep 18 09:11:24 2009 From: j.hough <@t> sheffield.ac.uk (julia hough) Date: Fri Sep 18 09:11:29 2009 Subject: [Histonet] zinc fixation form tibia Message-ID: <002701ca3869$e7ec1210$b7c43630$@hough@shef.ac.uk> Hello. Histonetters. I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also. I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were "jelly-like" before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. Thanks Julia From j.hough <@t> sheffield.ac.uk Fri Sep 18 09:12:23 2009 From: j.hough <@t> sheffield.ac.uk (julia hough) Date: Fri Sep 18 09:12:27 2009 Subject: [Histonet] (no subject) Message-ID: <002c01ca386a$0b276360$21762a20$@hough@shef.ac.uk> Dear Histonetters. I've been given the challenge of finding a variety of fluorochrome conjugated antibodies for IHC, however I'm having a great deal of trouble finding them. We basically want a fluorescent primary antibody that can be visualized instantly without use of a secondary etc. We would like antibodies to bind to Osteocalcin, Alkaline Phosphatase, Periostin, TRAP 5B, Cathepsin-K and N-Cadherin. If anyone has experience using fluorescent antibodies for the above or know of anyone who produces them I would be most grateful if you could let me know. Thanks Julia From j.hough <@t> sheffield.ac.uk Fri Sep 18 09:13:54 2009 From: j.hough <@t> sheffield.ac.uk (julia hough) Date: Fri Sep 18 09:13:58 2009 Subject: [Histonet] fluorescent antibodies for IHC Message-ID: <003101ca386a$418b0880$c4a11980$@hough@shef.ac.uk> Dear Histonetters. I've been given the challenge of finding a variety of fluorochrome conjugated antibodies for IHC, however I'm having a great deal of trouble finding them. We basically want a fluorescent primary antibody that can be visualized instantly without use of a secondary etc. We would like antibodies to bind to Osteocalcin, Alkaline Phosphatase, Periostin, TRAP 5B, Cathepsin-K and N-Cadherin. If anyone has experience using fluorescent antibodies for the above or know of anyone who produces them I would be most grateful if you could let me know. Thanks Julia From j.hough <@t> sheffield.ac.uk Fri Sep 18 09:31:47 2009 From: j.hough <@t> sheffield.ac.uk (julia hough) Date: Fri Sep 18 09:31:51 2009 Subject: [Histonet] f4/80 on wax embedded mouse tibia sections Message-ID: <003701ca386c$c0de54a0$429afde0$@hough@shef.ac.uk> Hello Histonetters. I have recently stained for f4/80 macrophage marker on wax embedded mouse tibia sections. I have managed to get staining however, I also have staining on my negative control, which seems quite specific. I have investigated this and there seems to be no problems with the DAB and all reagents used are exactly the same down to where they are bought as those recommended in a protocol. If you have managed to stain for f4/80 on tibia sections without getting staining on your control or you have any explanations for this I would much appreciate hearing from you to compare protocols. Thanks Julia From b-frederick <@t> northwestern.edu Fri Sep 18 09:44:00 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 18 09:44:14 2009 Subject: [Histonet] (no subject) In-Reply-To: <002c01ca386a$0b276360$21762a20$@hough@shef.ac.uk> Message-ID: <16D5B7F0292749EB90572810BC98D664@lurie.northwestern.edu> Try Jackson Immunoreasearch. We've gotten a lot from them for our double stains. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of julia hough Sent: Friday, September 18, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histonetters. I've been given the challenge of finding a variety of fluorochrome conjugated antibodies for IHC, however I'm having a great deal of trouble finding them. We basically want a fluorescent primary antibody that can be visualized instantly without use of a secondary etc. We would like antibodies to bind to Osteocalcin, Alkaline Phosphatase, Periostin, TRAP 5B, Cathepsin-K and N-Cadherin. If anyone has experience using fluorescent antibodies for the above or know of anyone who produces them I would be most grateful if you could let me know. Thanks Julia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Sep 18 10:11:28 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Sep 18 10:11:45 2009 Subject: [Histonet] RE: zinc fixative and decalcification with EDTA Message-ID: <000101ca3872$4d693c50$e83bb4f0$@callis@bresnan.net> You wrote: I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also. I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were "jelly-like" before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. **************************************************************************** **************************************************************************** **************************** Julie, By zinc fixation, I presume this is the Beckstead formalin free Zinc TRIS buffer mixture (ZSF)? If so, what you may have done is reverse the fixation by decalcifying the bone with EDTA rather than have the decal affected by the fixation. EDTA will chelate the zinc, along with all the other metal ions in bone e.g. calcium, magnesium, and iron. This very well may affect the condition of the bone after several weeks. I once again presume you are working with mouse bone? You did not provide some specific information here. I have not seen publications using this fixative for bone followed by decalcification, and it is a distinct possibility this will NOT work. I have some excellent publications on using ZSF for soft tissues. We do all our murine CD marker staining on undecalcified bone frozen sections after sectioning with Cryojane tape transfer system from Instrumedics using a tungsten carbide knife. Here are two publication references, freely accessible on J Histochem Cytochem to help you. I also have these in pdf and will send privately. Kim L. Kusser and Troy D. Randall Simultaneous Detection of EGFP and Cell Surface Markers by Fluorescence Microscopy in Lymphoid Tissues J. Histochem. Cytochem., Jan 2003; 51: 5 - 14. S Mori, T Sawai, T Teshima, and M Kyogoku A new decalcifying technique for immunohistochemical studies of calcified tissue, especially applicable to cell surface marker demonstration J. Histochem. Cytochem., Jan 1988; 36: 111 - 114. Gayle M. Callis HTL,HT,MT(ASCP) From gayle.callis <@t> bresnan.net Fri Sep 18 10:33:17 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Sep 18 10:33:29 2009 Subject: [Histonet] Re: Fluorescent antibodies for immunofluorescent staining and F4-80 Message-ID: <000601ca3875$59a64b40$0cf2e1c0$@callis@bresnan.net> Julia: You wrote (two separate messages) I've been given the challenge of finding a variety of fluorochrome conjugated antibodies for IHC, however I'm having a great deal of trouble finding them. We basically want a fluorescent primary antibody that can be visualized instantly without use of a secondary etc. We would like antibodies to bind to Osteocalcin, Alkaline Phosphatase, Periostin, TRAP 5B, Cathepsin-K and N-Cadherin. If anyone has experience using fluorescent antibodies for the above or know of anyone who produces them I would be most grateful if you could let me know. I have recently stained for f4/80 macrophage marker on wax embedded mouse tibia sections. I have managed to get staining however, I also have staining on my negative control, which seems quite specific. I have investigated this and there seems to be no problems with the DAB and all reagents used are exactly the same down to where they are bought as those recommended in a protocol. If you have managed to stain for f4/80 on tibia sections without getting staining on your control or you have any explanations for this I would much appreciate hearing from you to compare protocols. **************************************************************************** **************************************************************************** ****************************************** For fluorochrome conjugated primary antibodies, you can conjugate these on your own. Have you done a search for these antibodies via Google? Be aware the some fluorophore conjugated primaries might not fluoresce IF the antigens are in close proximity to each other. The antibody binds but the fluorochrome itself will not fluoresce due to a physical chemical phenomena called quenching. This is where the fluorophore trades electrons instead, quenching any fluorescence you want to see. If this happens , you would have to detect the fluorophore with antibody, eg. antiFITC, anti Alexa 488 and so on. You are back to square one then. Often it is better to just detect the antibody with a secondary-conjugated to fluorophores, or buy a biotinylated primary antibody and detect with Streptavidin Alexa dye (fluorophore). We do triple IF work for murine CD markers that does not take days to perform, and double IF is very straightforward. As for the F4/80 antibody, you did not provide YOUR protocol so people can respond to the question - fixation, decalcification, retrieval, normal serum blocking, primary and negative control concentrations, etc. This antibody works on FFPE tissue, and there are several postings on Histonet (go to Archives) on protocols for successful staining for soft tissues. The negative control should be negative though. Did you do a dilution panel to determine working concentration of primary? What was secondary? Gayle M. Callis HTL,HT,MT(ASCP) From b-frederick <@t> northwestern.edu Fri Sep 18 10:33:22 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 18 10:34:16 2009 Subject: [Histonet] Ultra-Low Temp. Upright Freezer In-Reply-To: Message-ID: <5A27C387DF7D4343BC5F44B1A128B0DF@lurie.northwestern.edu> We have 20 freezers. The preference here by the tissue bank staff is the Sanyo VIP(MDF-U473 VC). Though it warms faster, the recovery time is also faster than the other ones (Revco,Kelvinator etc. The staff also note that it holds more than due to the thinner liner. WE also have an electronic lock on it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, September 17, 2009 8:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ultra-Low Temp. Upright Freezer Hi all! I'm hoping to get some help RE: purchasing a new ultra-low temp. upright freezer. I've tried searching Histonet, but many of the posts are a few years old, and my guess is that the products have changed a bit over the years. My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., but preferably much larger), and definitely upright (not chest) -80. Unfortunately, since this is my first foray into the world of lab freezers, I know very little about them. I have discovered that Thermo's Revco and Sanyo freezers seem to be popular. Obviously we're looking for a freezer that is as large and efficient as possible. Insulation and the freezer's compressor(s) are most important. I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) freezer seems quite nice - small exterior footprint and his sales pitch regarding the vacuum insulation (therefore denser insulation), insulated inner doors, and supposed minimized carbon footprint was quite convincing. I still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, though my guess is that their sales pitch will be just as convincing. Since you can't depend only on sales pitches, I'm depending on colleagues as well.... Does anyone have any experience with either the Sanyo or Revco freezers (or any others that they'd like to recommend)? Any and all positive and negative feedback would be greatly appreciated! I'm hoping to get comments regarding: - efficiency - how often service is needed due to failures - are the alarms sensitive enough or maybe too sensitive - how loud the freezer is when the compressor is on - etc...... As always, please remember to Reply All. Thank you in advance for your help! I look forward to hearing everyone's suggestions. Lucie UCSD lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Fri Sep 18 12:26:42 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Sep 18 12:26:46 2009 Subject: [Histonet] Re: Polarizing filters Message-ID: I am really appalled by this thread. In any specialty but pathology, a physician would not be required to go to a camera store for his optical equipment, or look through polarizing sun glasses at a specimen. But it's what's out there - very few pathologists I work for have polarizers on their microscopes. A pathologist's microscope should have built-in polarization, and the system should include a first order plate (full wave plate) for determining the birefringence direction of crystals (needed for identifying monosodium urate, a common diagnostic question). When I was ten years old in 1949 I had a polarizer on my Dad's 1923 medical school monocular brass tube Leitz - I wish I had that scope today (my sister has it, in a garage in L.A.) (I was an avid rockhound - still am.) Why can't I have one 60 years later? I don't mind buying my bone saws from Home Depot and my OptiVISION magnifier from Amazon, but when it comes to my microscope I'd sort of like to have the real thing. Bob Richmond Samurai Pathologist Knoxville TN From kenneth.metzger <@t> aruplab.com Fri Sep 18 12:29:22 2009 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Fri Sep 18 12:29:40 2009 Subject: [Histonet] Whole Mount Coverslips Message-ID: Can anyone tell me who sells whole mount coverslips besides Brain Research Labs? They have mine back-ordered until 10/8/09 and I need some before then. Thanks, Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From POWELL_SA <@t> mercer.edu Fri Sep 18 12:44:56 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Sep 18 12:45:01 2009 Subject: [Histonet] RE: Whole Mount Coverslips In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE21B58759F3A@MERCERMAIL.MercerU.local> Hi Kenneth, Most any company that makes slides or coverslips can make you the size you need. Not sure how long it would take but there are more than one source. Try Thermo FIsher reps, they bought out Richard Alan who used to make any size needed. Erie who makes glass slides and coverslips I think was bought out by ehm too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, September 18, 2009 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole Mount Coverslips Can anyone tell me who sells whole mount coverslips besides Brain Research Labs? They have mine back-ordered until 10/8/09 and I need some before then. Thanks, Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janderson <@t> halozyme.com Fri Sep 18 12:49:54 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Fri Sep 18 12:50:00 2009 Subject: [Histonet] black plastic incubation boxes Message-ID: <5F7CC9B788911848A79BC83453A3D653028842D70D@Tomlinson.hti.com> Hello Histonet! I have a large black square molded plastic box ( about 15"x15"x2") that I use for IHC. It holds 30 slides (10 slides on 3 sticky rails). We have smaller boxes (15"x7"x2") of the same molded plastic that I can order from American Master Tech (holds 20 slides) but I want another large square box. Does anyone have an idea where I can find one? Thanks a lot! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From ratliffjack <@t> hotmail.com Fri Sep 18 13:08:22 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Sep 18 13:08:30 2009 Subject: [Histonet] Whole Mount Coverslips In-Reply-To: References: Message-ID: What size do you need? Jack Ratliff > Date: Fri, 18 Sep 2009 11:29:22 -0600 > From: kenneth.metzger@aruplab.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Whole Mount Coverslips > > Can anyone tell me who sells whole mount coverslips besides Brain > Research Labs? They have mine back-ordered until 10/8/09 and I need some > before then. Thanks, > > > > Ken > > > > Kenneth G Metzger HTL(ASCP) > > ARUP Labs > > Histology Supervisor > > 500 Chipeta Way > > Salt Lake City, Utah 84108-1221 > > kenneth.metzger@aruplab.com > > (801) 583-2787 x 3101 > > > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Fri Sep 18 16:00:04 2009 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Sep 18 16:00:10 2009 Subject: [Histonet] Ultra-Low Temp. Upright Freezer Message-ID: Hi Lucie, My research group has 63 Revco (or Harris-same brand) upright freezers of various ages at my location. All are 21 to 25 cu ft. Within my organization, there are about 200 on three campuses. The older ones break down with some regularity, (maybe 2/63 per year), so my biomed tech insists on Revco. We have not lost an upright freezer, and every unit that goes down has been repaired. We do not have any affiliation with Revco. We have purchased 7 in the last 4 years, and I have to admit, 2 years ago, several new units were flawed (motors not in synch[?] and never attained -80C). However, the units were repaired and the newer units appear flawless. Their alarms are adjustable (ie. "Warm point alarm") so we do not have problems with over or under sensitivity. How loud? They are fairly loud, but tolerable as a moderate hum. We have several units interspersed throughout the labs, and these single units are fine. However, the thermal release from the motors in smaller room with multiple freezers (side-by-side) is significant and given these two factors in this scinerio, we keep the area around them clear. Most of the hospitals I work with have Revco's too. Several are lay-down, but most are upright. The lay-down units seem to have fewer problems with frost, but take up a larger footprint. They have them in the work areas with seemingly no problems. The Sanyo unit looks good and if my biomed tech would not have killed me (did not mean literally), I would have purchased them. I think they are less expensive and come from a dependable source (in my opinion). We just didn't know about replacement parts to my area. Hope this helps and good luck with your search, Hugh Luk, HTL (ASCP) Pathology shared resource lab manager Cancer research center of Hawaii > From: lucie.s.guernsey@gmail.com > Date: Thu, 17 Sep 2009 18:15:15 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ultra-Low Temp. Upright Freezer > > Hi all! > > I'm hoping to get some help RE: purchasing a new ultra-low temp. upright > freezer. I've tried searching Histonet, but many of the posts are a few > years old, and my guess is that the products have changed a bit over the > years. > > My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., > but preferably much larger), and definitely upright (not chest) -80. > Unfortunately, since this is my first foray into the world of lab freezers, > I know very little about them. I have discovered that Thermo's Revco and > Sanyo freezers seem to be popular. Obviously we're looking for a freezer > that is as large and efficient as possible. Insulation and the freezer's > compressor(s) are most important. > > I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) > freezer seems quite nice - small exterior footprint and his sales pitch > regarding the vacuum insulation (therefore denser insulation), insulated > inner doors, and supposed minimized carbon footprint was quite convincing. I > still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, > though my guess is that their sales pitch will be just as convincing. > > Since you can't depend only on sales pitches, I'm depending on colleagues as > well.... Does anyone have any experience with either the Sanyo or Revco > freezers (or any others that they'd like to recommend)? Any and all positive > and negative feedback would be greatly appreciated! I'm hoping to get > comments regarding: > - efficiency > - how often service is needed due to failures > - are the alarms sensitive enough or maybe too sensitive > - how loud the freezer is when the compressor is on > - etc...... > > As always, please remember to Reply All. > > Thank you in advance for your help! I look forward to hearing everyone's > suggestions. > > > Lucie > > UCSD > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Lauren found her dream laptop. Find the PC that?s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290 From aazath <@t> hotmail.com Sun Sep 20 04:50:38 2009 From: aazath <@t> hotmail.com (aazath@hotmail.com) Date: Sun Sep 20 04:46:25 2009 Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 24 Message-ID: Dear All, I am using Haris's hematoxylin.some times its weak.Is any body have method to get a strong hematoxylin consistently. Aazath Department of pathology Apollo hospitals. -original message- Subject: Histonet Digest, Vol 70, Issue 24 From: histonet-request@lists.utsouthwestern.edu Date: 19/09/2009 10:30 pm Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Polarizing filters (Robert Richmond) 2. Whole Mount Coverslips (Metzger, Kenneth) 3. RE: Whole Mount Coverslips (Shirley A. Powell) 4. black plastic incubation boxes (Jennifer Anderson) 5. RE: Whole Mount Coverslips (Jack Ratliff) 6. RE: Ultra-Low Temp. Upright Freezer (Hugh Luk) ---------------------------------------------------------------------- Message: 1 Date: Fri, 18 Sep 2009 13:26:42 -0400 From: Robert Richmond Subject: [Histonet] Re: Polarizing filters To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I am really appalled by this thread. In any specialty but pathology, a physician would not be required to go to a camera store for his optical equipment, or look through polarizing sun glasses at a specimen. But it's what's out there - very few pathologists I work for have polarizers on their microscopes. A pathologist's microscope should have built-in polarization, and the system should include a first order plate (full wave plate) for determining the birefringence direction of crystals (needed for identifying monosodium urate, a common diagnostic question). When I was ten years old in 1949 I had a polarizer on my Dad's 1923 medical school monocular brass tube Leitz - I wish I had that scope today (my sister has it, in a garage in L.A.) (I was an avid rockhound - still am.) Why can't I have one 60 years later? I don't mind buying my bone saws from Home Depot and my OptiVISION magnifier from Amazon, but when it comes to my microscope I'd sort of like to have the real thing. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Fri, 18 Sep 2009 11:29:22 -0600 From: "Metzger, Kenneth" Subject: [Histonet] Whole Mount Coverslips To: Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone tell me who sells whole mount coverslips besides Brain Research Labs? They have mine back-ordered until 10/8/09 and I need some before then. Thanks, Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ------------------------------ Message: 3 Date: Fri, 18 Sep 2009 13:44:56 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: Whole Mount Coverslips To: "Metzger, Kenneth" , "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <9BF995BC0E47744E9673A41486E24EE21B58759F3A@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" Hi Kenneth, Most any company that makes slides or coverslips can make you the size you need. Not sure how long it would take but there are more than one source. Try Thermo FIsher reps, they bought out Richard Alan who used to make any size needed. Erie who makes glass slides and coverslips I think was bought out by ehm too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, September 18, 2009 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole Mount Coverslips Can anyone tell me who sells whole mount coverslips besides Brain Research Labs? They have mine back-ordered until 10/8/09 and I need some before then. Thanks, Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 18 Sep 2009 10:49:54 -0700 From: Jennifer Anderson Subject: [Histonet] black plastic incubation boxes To: "histonet@lists.utsouthwestern.edu" Message-ID: <5F7CC9B788911848A79BC83453A3D653028842D70D@Tomlinson.hti.com> Content-Type: text/plain; charset="us-ascii" Hello Histonet! I have a large black square molded plastic box ( about 15"x15"x2") that I use for IHC. It holds 30 slides (10 slides on 3 sticky rails). We have smaller boxes (15"x7"x2") of the same molded plastic that I can order from American Master Tech (holds 20 slides) but I want another large square box. Does anyone have an idea where I can find one? Thanks a lot! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ------------------------------ Message: 5 Date: Fri, 18 Sep 2009 14:08:22 -0400 From: Jack Ratliff Subject: RE: [Histonet] Whole Mount Coverslips To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" What size do you need? Jack Ratliff > Date: Fri, 18 Sep 2009 11:29:22 -0600 > From: kenneth.metzger@aruplab.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Whole Mount Coverslips > > Can anyone tell me who sells whole mount coverslips besides Brain > Research Labs? They have mine back-ordered until 10/8/09 and I need some > before then. Thanks, > > > > Ken > > > > Kenneth G Metzger HTL(ASCP) > > ARUP Labs > > Histology Supervisor > > 500 Chipeta Way > > Salt Lake City, Utah 84108-1221 > > kenneth.metzger@aruplab.com > > (801) 583-2787 x 3101 > > > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 18 Sep 2009 11:00:04 -1000 From: Hugh Luk Subject: RE: [Histonet] Ultra-Low Temp. Upright Freezer To: , histonet Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi Lucie, My research group has 63 Revco (or Harris-same brand) upright freezers of various ages at my location. All are 21 to 25 cu ft. Within my organization, there are about 200 on three campuses. The older ones break down with some regularity, (maybe 2/63 per year), so my biomed tech insists on Revco. We have not lost an upright freezer, and every unit that goes down has been repaired. We do not have any affiliation with Revco. We have purchased 7 in the last 4 years, and I have to admit, 2 years ago, several new units were flawed (motors not in synch[?] and never attained -80C). However, the units were repaired and the newer units appear flawless. Their alarms are adjustable (ie. "Warm point alarm") so we do not have problems with over or under sensitivity. How loud? They are fairly loud, but tolerable as a moderate hum. We have several units interspersed throughout the labs, and these single units are fine. However, the thermal release from the motors in smaller room with multiple freezers (side-by-side) is significant and given these two factors in this scinerio, we keep the area around them clear. Most of the hospitals I work with have Revco's too. Several are lay-down, but most are upright. The lay-down units seem to have fewer problems with frost, but take up a larger footprint. They have them in the work areas with seemingly no problems. The Sanyo unit looks good and if my biomed tech would not have killed me (did not mean literally), I would have purchased them. I think they are less expensive and come from a dependable source (in my opinion). We just didn't know about replacement parts to my area. Hope this helps and good luck with your search, Hugh Luk, HTL (ASCP) Pathology shared resource lab manager Cancer research center of Hawaii > From: lucie.s.guernsey@gmail.com > Date: Thu, 17 Sep 2009 18:15:15 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ultra-Low Temp. Upright Freezer > > Hi all! > > I'm hoping to get some help RE: purchasing a new ultra-low temp. upright > freezer. I've tried searching Histonet, but many of the posts are a few > years old, and my guess is that the products have changed a bit over the > years. > > My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., > but preferably much larger), and definitely upright (not chest) -80. > Unfortunately, since this is my first foray into the world of lab freezers, > I know very little about them. I have discovered that Thermo's Revco and > Sanyo freezers seem to be popular. Obviously we're looking for a freezer > that is as large and efficient as possible. Insulation and the freezer's > compressor(s) are most important. > > I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) > freezer seems quite nice - small exterior footprint and his sales pitch > regarding the vacuum insulation (therefore denser insulation), insulated > inner doors, and supposed minimized carbon footprint was quite convincing. I > still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, > though my guess is that their sales pitch will be just as convincing. > > Since you can't depend only on sales pitches, I'm depending on colleagues as > well.... Does anyone have any experience with either the Sanyo or Revco > freezers (or any others that they'd like to recommend)? Any and all positive > and negative feedback would be greatly appreciated! I'm hoping to get > comments regarding: > - efficiency > - how often service is needed due to failures > - are the alarms sensitive enough or maybe too sensitive > - how loud the freezer is when the compressor is on > - etc...... > > As always, please remember to Reply All. > > Thank you in advance for your help! I look forward to hearing everyone's > suggestions. > > > Lucie > > UCSD > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Lauren found her dream laptop. Find the PC that?s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 70, Issue 24 **************************************** From brian <@t> prometheushealthcare.com Sun Sep 20 14:51:29 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Sun Sep 20 14:51:34 2009 Subject: [Histonet] Histotech Openings in New York Message-ID: The following histology positions are available throughout NY. We are offering up to $1,000 for referrals if we place them. Any help would be appreciated. Thank you! 1) Hospital in NY, NY Multiple shifts available for techs 2) Privately held lab in Brooklyn, NY for early day shift tech 3) NYC Hospital night histotechnologist positions Histology Supervisor 4) Hospital in Brooklyn Histology Supervisor 5) Reference lab in Port Chester Night shift tech opening 6) Hospital in New Rochelle Histology Supervisor/ Pathology Assistant ****Immediate need!*** 7) Reference lab in Westchester County Multiple openings for techs, grossing techs, and night PA opening 8) Reference lab in Syosset Night tech opening Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From AnthonyH <@t> chw.edu.au Sun Sep 20 18:20:48 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 20 18:21:00 2009 Subject: [Histonet] zinc fixation form tibia In-Reply-To: <002701ca3869$e7ec1210$b7c43630$@hough@shef.ac.uk> Message-ID: Julia, Does the zinc-fix contain formalin? If not then the tissue may not protected from the effects of decalcification (even the mild EDTA decal you used)and so cells may be leached/destroyed in the tissue. or Do you use enzyme retrieval? This will also affect the cells if formalin fixation is not adequate. HIER may not be affected as much. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of julia hough Sent: Saturday, 19 September 2009 12:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] zinc fixation form tibia Hello. Histonetters. I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also. I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were "jelly-like" before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. Thanks Julia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From nefff <@t> staff.uni-marburg.de Mon Sep 21 07:23:39 2009 From: nefff <@t> staff.uni-marburg.de (Dr. Frauke Neff) Date: Mon Sep 21 07:23:54 2009 Subject: [Histonet] indian ink- thank you Message-ID: <20090921142339.dccrlfwjk0wo0wow@home.staff.uni-marburg.de> Dear everyone, I just wanted to thank you for all the tips concerning the use of indian ink. They helped a lot! Vielen Dank, Frauke From TJJ <@t> stowers.org Mon Sep 21 08:57:13 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Sep 21 08:58:14 2009 Subject: [Histonet] Re: Harris hematoxylin weak staining Message-ID: Aazath, There are several strategies you can use to improve your hematoxylin staining. 1 - use distilled or DI water before putting your slides into the stain. Sometimes the chemicals/minerals/metals in the tap water can weaken your stain. 2 - replace your stains when they start showing signs of weakening, or routinely in order to keep the quality even. 3 - try using more than one bucket/dish of hematoxylin and split the total staining time between them. Then you can rotate the last one up to the first position, and put a new change in the second station. This might help keep your staining more consistent. 4 - does your tap water seem to contain a high level of chlorine? If so, you can use a bluing agent to blue your stained slides, and then use DI or distilled water rinses. 5 - do not linger in the decolorizing acid alcohol. Usually one quick dip followed by an immediate plunge into water is all you need. 6 - some have reported that their eosin, if the pH drops too low, can leach out some of the hematoxylin. According to Carson, the ideal pH of eosin is between 4.6 and 5. 7 - what thickness is your tissue? 3 microns thin tissue needs longer in the stain solution than 5 microns thin tissue. There may be others that folks will chime in with, but these are the most obvious ones to me. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From mucram11 <@t> comcast.net Mon Sep 21 09:09:54 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Sep 21 09:09:59 2009 Subject: [Histonet] Re: Harris hematoxylin weak staining In-Reply-To: Message-ID: <1330549245.144801253542194388.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> If you bleach your staining dishes they must be rinsed to excess or some bleach may remain in the container and effect you staining as it leaches into the solution.? Check to be sure everyone takes the time to do this as I have had an issue where one or more people in the lab were more careful tham others about this step.? Using Harris requires filtering so the type of filter paper can also cause an issue.? Use a more porous paper. Pam Marcum ----- Original Message ----- From: "Teri Johnson" < TJJ @ stowers .org> To: " histonet " < histonet @lists. utsouthwestern . edu > Sent: Monday, September 21, 2009 9:57:13 AM GMT -05:00 US/Canada Eastern Subject: [ Histonet ] Re: Harris hematoxylin weak staining Aazath , There are several strategies you can use to improve your hematoxylin staining. 1 - use distilled or DI water before putting your slides into the stain. Sometimes the chemicals/minerals/metals in the tap water can weaken your stain. 2 - replace your stains when they start showing signs of weakening, or routinely in order to keep the quality even. 3 - try using more than one bucket/dish of hematoxylin and split the total staining time between them. Then you can rotate the last one up to the first position, and put a new change in the second station. This might help keep your staining more consistent. 4 - does your tap water seem to contain a high level of chlorine? If so, you can use a bluing agent to blue your stained slides, and then use DI or distilled water rinses. 5 - do not linger in the decolorizing acid alcohol. Usually one quick dip followed by an immediate plunge into water is all you need. 6 - some have reported that their eosin , if the pH drops too low, can leach out some of the hematoxylin . According to Carson, the ideal pH of eosin is between 4.6 and 5. 7 - what thickness is your tissue? 3 microns thin tissue needs longer in the stain solution than 5 microns thin tissue. There may be others that folks will chime in with, but these are the most obvious ones to me. Good luck! Teri Johnson, HT( ASCP ) QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From kadamsplw <@t> gmail.com Mon Sep 21 09:32:35 2009 From: kadamsplw <@t> gmail.com (karen adams) Date: Mon Sep 21 09:32:41 2009 Subject: [Histonet] Prostate processing Message-ID: Prostate Laboratories.....Would you PLEASE fax us a copy of your processing protocol??? We would be SO greatful. Also....curious to know if you are utilizing IBF fixative and if you have had to change staining times as some of our pathologists are saying staining is too pale compared to formalin fixed H & E. Thank you, Thank you!!!! -- K. Leigh Adams, MS, HTL (ASCP) Histology Laboratory Tennessee Urology Associates 800 Oak Ridge TPKE/STE A206 Oak Ridge, TN 37830 (865) 483-1800 FAX (865) 482-2001 kadamsplw@gmail.com From Bonnie.Whitaker <@t> osumc.edu Mon Sep 21 11:19:20 2009 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Mon Sep 21 11:19:33 2009 Subject: [Histonet] Workload value of whole mount embedding and cutting Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D666DD@msxc06.OSUMC.EDU> Hi Histonetters, Could someone that does whole mount specimens share how you count them for workload purposes, as compared to reqular embedding and cutting? (Prostates are the only thing that we currently do as whole mount specimens.) Thanks! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 From rjbuesa <@t> yahoo.com Mon Sep 21 11:31:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 21 11:31:55 2009 Subject: [Histonet] Workload value of whole mount embedding and cutting In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D666DD@msxc06.OSUMC.EDU> Message-ID: <384446.64012.qm@web65701.mail.ac4.yahoo.com> I used to assign to each whole prostate block the equivalent of 3 blocks while embedding and 10 blocks when cutting with our sledge microtome. Ren? J. --- On Mon, 9/21/09, Whitaker, Bonnie wrote: From: Whitaker, Bonnie Subject: [Histonet] Workload value of whole mount embedding and cutting To: histonet@lists.utsouthwestern.edu Date: Monday, September 21, 2009, 12:19 PM Hi Histonetters, Could someone that does whole mount specimens share how you count them for workload purposes, as compared to reqular embedding and cutting?? (Prostates are the only thing that we currently do as whole mount specimens.) Thanks! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Mon Sep 21 11:53:23 2009 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Mon Sep 21 11:53:34 2009 Subject: [Histonet] bone marrow assisting? Message-ID: <2925AE271EAAD440AF48FCCEB8002D0908B81196@smgmail01.smgj.sclhs.net> I am looking for some info on technique at bone marrow procedures? Are aspirates being dropped from the syringe onto the slides or onto a petri dish and only marrow with spicules transferred to slides or something else? ?? Is the syringe heparanized?? Thank you in advance Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rsrichmond <@t> gmail.com Mon Sep 21 12:33:26 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Sep 21 12:33:33 2009 Subject: [Histonet] Re: bone marrow assisting? Message-ID: Anna Inman at St. Mary's Hospital, Grand Junction CO asks: >>I am looking for some info on technique at bone marrow procedures. - Are aspirates being dropped from the syringe onto the slides or onto a petri dish and only marrow with spicules transferred to slides or something else? ?? - Is the syringe heparinized??<< If you don't know how to do this, it's your pathologist's job to teach you how. Grump. We're talking about a posterior iliac crest biopsy, with a core of bone and a syringe full of marrow particles (not spicules) and blood. There are a lot of ways to do this. A very simple method: set a glass slide at about a 15 degree slant. Squirt some of the specimen out of the syringe onto the high end of the slide. The blood will run down the slide, leaving the marrow particles loosely adhering to the slide. Pick up particles one at a time, using a wooden applicator stick broken with the grain to form a tiny flat scoop. Spread and smear each particle between two slides or square coverslips, for as many slides as you want. Heparin isn't needed, and may damage the specimen. If you use it, use only a very small quantity. The remaining specimen may be allowed to clot in the syringe, removed, and fixed for clot sections. I prefer to squirt the remainder of the specimen, not yet clotted, into neutral buffered formalin, and later filter the fixed particles out and embed them in a paraffin block. Why look at (or cut?) all that clot? The person performing the marrow biopsy should not be the same person who does the preparation - it's a two person job. If you don't see any particles on the slide, inform the person doing the aspiration so they can repeat the aspiration. (Some biopsy-ers never quite get the hang of this.) Don't B.S. me - if I haven't got any marrow particles, I want to know! While tracking down "stmarygj" I found that the hospital has an institute inspired by Geno Saccomanno, the inventor of the well-known cytology fixative. http://www.stmarygj.com/body.cfm?id=61 Bob Richmond Samurai Pathologist Knoxville TN From wlecorch <@t> rwjuhh.edu Mon Sep 21 12:50:00 2009 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Mon Sep 21 12:50:52 2009 Subject: [Histonet] bone marrow assisting? Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB789FB@HAMEXMBA.rwjham.local> The current protocol that we follow here is as follows: Two green tops are obtained first thing for Flow and Cyto followed by eight smears, from that same syringe then draw air in and place on its side allow a clot to form. From the bone bx make a touch prep rolling the bone between two slides prior to dropping the bone in 10%NBF all slides are air dried. You can pick out the best slide for iron and the rest get a Giemsa stain. From sbreeden <@t> nmda.nmsu.edu Mon Sep 21 13:22:37 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Sep 21 13:22:44 2009 Subject: [Histonet] OT: Early Friday Hour of Fuming Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46B00@nmdamailsvr.nmda.ad.nmsu.edu> I have just found out this morning that I will be unable to attend NSH this year. This alone is unfortunate news for me but, interestingly enough, is not due to budget issues. The reason I am "fuming" this morning is not because there is no funding for my attendance (that was already in place), but because there is such a serious shortage of histotechs in this country that there is no one to cover me! A "traveler" is out of the question (that's a budget issue!); anyone that might be hiding and is not known to me to be available in our city would have to have about a month's lead time to get vetted for the position through the State. Anyone that thinks that histology is not important should try to fill a position when there is such a shortage of trained and qualified technicians. If we, as histologists, do not begin to make ourselves more visible and promote our profession as a worthy, fulfilling and job-secure life, more and more of us will find ourselves in this position. Thank you - I feel better for having Fumed. There is no need to reply as I know many of us are in the same boat. I was in the boat and lost my paddle! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lpaveli1 <@t> hurleymc.com Mon Sep 21 13:56:10 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Sep 21 13:56:27 2009 Subject: [Histonet] Ultra fast pap stain protocol Message-ID: <4AB7940A020000EE0002CA6F@smtp-gw.hurleymc.com> Hello, Does anyone have a protocol for the Ultra-fast pap stain used on FNA's?? Thanks for your help, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 From mfisher <@t> ecrmc.org Mon Sep 21 14:10:35 2009 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Mon Sep 21 14:10:39 2009 Subject: [Histonet] Night Blue for AFB Message-ID: <3ACBB5D73A417547A01970CD3EB550930314A96E@MAIL1.ecrmc.ci.el-centro.ca.us> Does anyone know where to purchase the dye Night Blue? I used this stain for AFB many years ago (1986) and would like to introduce it here. Thank you. M. Fisher El Centro Regional Medical Center El Centro, CA From rjr6 <@t> psu.edu Mon Sep 21 14:36:16 2009 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Mon Sep 21 14:36:41 2009 Subject: [Histonet] RE: OT: Early Friday Hour of Fuming In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46B00@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46B00@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: That's alright whenever I take vacation I come back to all the work sitting here waiting for me. I just took 2 weeks off and it took 3 weeks to catch up so I feel your pain. Roberta Horner Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, September 21, 2009 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Early Friday Hour of Fuming I have just found out this morning that I will be unable to attend NSH this year. This alone is unfortunate news for me but, interestingly enough, is not due to budget issues. The reason I am "fuming" this morning is not because there is no funding for my attendance (that was already in place), but because there is such a serious shortage of histotechs in this country that there is no one to cover me! A "traveler" is out of the question (that's a budget issue!); anyone that might be hiding and is not known to me to be available in our city would have to have about a month's lead time to get vetted for the position through the State. Anyone that thinks that histology is not important should try to fill a position when there is such a shortage of trained and qualified technicians. If we, as histologists, do not begin to make ourselves more visible and promote our profession as a worthy, fulfilling and job-secure life, more and more of us will find ourselves in this position. Thank you - I feel better for having Fumed. There is no need to reply as I know many of us are in the same boat. I was in the boat and lost my paddle! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Mon Sep 21 15:16:23 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Sep 21 15:16:28 2009 Subject: [Histonet] bone marrow assisting? In-Reply-To: <09411E0112A96A459D8D5FBDAB9C15C71A4AB789FB@HAMEXMBA.rwjham.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3C1B@is-e2k3.grhs.net> Same here! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lecorchick, William Sent: Monday, September 21, 2009 12:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone marrow assisting? The current protocol that we follow here is as follows: Two green tops are obtained first thing for Flow and Cyto followed by eight smears, from that same syringe then draw air in and place on its side allow a clot to form. From the bone bx make a touch prep rolling the bone between two slides prior to dropping the bone in 10%NBF all slides are air dried. You can pick out the best slide for iron and the rest get a Giemsa stain. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thecitan <@t> yahoo.com Mon Sep 21 15:26:34 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Mon Sep 21 15:23:27 2009 Subject: [Histonet] Separation Artifact Message-ID: <227015874-1253564600-cardhu_decombobulator_blackberry.rim.net-1731111324-@bda958.bisx.prod.on.blackberry> Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe h&e skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry From AnthonyH <@t> chw.edu.au Mon Sep 21 19:43:31 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 21 19:43:42 2009 Subject: [Histonet] Separation Artifact In-Reply-To: <227015874-1253564600-cardhu_decombobulator_blackberry.rim.net-1731111324-@bda958.bisx.prod.on.blackberry> Message-ID: Dear thecitan@yahoo.com (whoever this is) I would suggest that to fix the problem, make sure you fix the tissues. Shrinkage is often due to alcohol "fixation" rather than formal fixation Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Tuesday, 22 September 2009 6:27 AM To: Histonet Subject: [Histonet] Separation Artifact Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe h&e skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Sep 22 02:59:50 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Sep 22 02:59:56 2009 Subject: [Histonet] Separation Artifact References: <227015874-1253564600-cardhu_decombobulator_blackberry.rim.net-1731111324-@bda958.bisx.prod.on.blackberry> Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07A1FD46@wahtntex2.waht.swest.nhs.uk> If its happening to an entire batch but not to other batches then you can sorta rule out fixation unless you use different batches of fixative for different batches of samples; logical yes? To put it another way if you use the same fixative and fixation time on all batches but only one is affected then it must be an issue with the machine. Not knowing which one you are using makes this difficult but sometimes they go wrong or there's the wrong fluid dispensed. If however you are using different batches of fixatives on different batches of samples you may have one batch of fixative that's incorrectly made up. Hope that helps. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: 21 September 2009 21:27 To: Histonet Subject: [Histonet] Separation Artifact Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe h&e skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Tue Sep 22 09:13:47 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Sep 22 09:13:58 2009 Subject: [Histonet] (no subject) Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7654@wlmmsx01.nemours.org> Histo-netters? Looking for the best method to process some mouse eyes in paraffin. Let me know about the fix, too...(.if it is something exotic?) I have a project coming my way....and I am sure there is someone out there who has done this a million times...and I don't need to re-invent the wheel for somethng I most likely will do once. I know the experts are out there, already! Thanks! CB From ian.montgomery <@t> bio.gla.ac.uk Tue Sep 22 09:30:56 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Sep 22 09:34:17 2009 Subject: [Histonet] Enteric Nervous System. Message-ID: <12EB9B60C9474D7B9DC6E33EFF370470@IBLS.GLA.AC.UK> Start of October I'm giving a lecture on the enteric nervous system and I'm short of a few images. I've got beautiful micrographs of the neurones stained with silver and conventional stains, but lack some IHC of various neuropeptides either in sections or 'en face' As it's the start of the semester time is not on my side trying to prepare some specimens for this class, so, can anyone help? Species, I don't care, brightfield or fluorescence, just as long as it looks nice for the class. Naturally you'll get full recognition for your work. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From jessgrocki <@t> yahoo.com Tue Sep 22 10:32:39 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Sep 22 10:32:46 2009 Subject: [Histonet] Expired Antibodies for Research Message-ID: <908530.64708.qm@web82008.mail.mud.yahoo.com> Hello From JWeems <@t> sjha.org Tue Sep 22 10:47:20 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Sep 22 10:47:06 2009 Subject: [Histonet] Asbestos Body Digestion Message-ID: Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From laurie.colbert <@t> huntingtonhospital.com Tue Sep 22 10:59:40 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Sep 22 10:59:45 2009 Subject: [Histonet] Large cassettes Message-ID: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> My pathologist wants to sample a particular feature of a baby's brain (autopsy), but the brain is too soft to cut and still maintain this feature. She wants to process a very large section of the brain, and then cut it down after it has been processed and has some firmness to it. Does anyone know where I can get a large cassette - approximately 4" x 4" - as a sample? I don't want to have to buy a large quantity, because this is probably a one-time thing. I'm not sure such a cassette even exists, but it seems like I've seen one before. Laurie Colbert Huntington Hospital Pasadena, CA From BBranton <@t> sarapath.com Tue Sep 22 11:25:57 2009 From: BBranton <@t> sarapath.com (Brian Branton) Date: Tue Sep 22 11:26:08 2009 Subject: [Histonet] Equipment for Sale Message-ID: Hello HistoNetters, I have some equipment for sale. I have one Ventana Benchmark XT with three Nexus IHC stainers for sale. I have a number of Sakura Tissue Storage Cabinets for sale. Finally, I have an Epson LQ-590 printer for sale. If anyone is interested, please see our web site at http://www.sarapath.com/equipment4sale Thanks, Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX From contact <@t> excaliburpathology.com Tue Sep 22 11:50:34 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Sep 22 11:50:39 2009 Subject: [Histonet] Large cassettes In-Reply-To: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> Message-ID: <655216.34693.qm@web1101.biz.mail.sk1.yahoo.com> Hi, I process large portions of?tissue and eyes. There are no cassettes?as large as 4"x4". Believe me I have been looking for 30 years. But, what?I use are soap molds, plastic tubs, L-blocks, etc. Paula ________________________________ From: Laurie Colbert To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 22, 2009 10:59:40 AM Subject: [Histonet] Large cassettes My pathologist wants to sample a particular feature of a baby's brain (autopsy), but the brain is too soft to cut and still maintain this feature.? She wants to process a very large section of the brain, and then cut it down after it has been processed and has some firmness to it.? Does anyone know where I can get a large cassette - approximately 4" x 4" - as a sample?? I don't want to have to buy a large quantity, because this is probably a one-time thing.? I'm not sure such a cassette even exists, but it seems like I've seen one before. Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Tue Sep 22 11:52:24 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Sep 22 11:52:36 2009 Subject: [Histonet] Asbestos Body Digestion In-Reply-To: References: Message-ID: <4AB8C888.59CD.00EE.0@hurleymc.com> I've attached our protocol that we have successfully used in the past. Prior to using this procedure, I would deparaffinize your block, taking it down to water. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Weems, Joyce" 9/22/2009 11:47 AM >>> Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Sep 22 12:15:39 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Sep 22 12:15:43 2009 Subject: [Histonet] Re: Night Blue for AFB Message-ID: Marcia Fisher at El Centro [CA] Regional Medical Center asks: >>Does anyone know where to purchase the dye Night Blue? I used this stain for AFB many years ago (1986) and would like to introduce it here.<< I don't know whether it's still available. Victoria Blue R was used for this same purpose, and it may still be available. These blue acid-fast stains were sometimes used by color-blind pathologists, who cannot distinguish red acid-fast bacilli in a blue background. Light microscopy - red or blue - for AFB has been obsolete at least since the 1960's - fluorescent methods (auramine) are much more sensitive. Pathologists continue to use them, though. I think that light microscopic AFB stains should be banned by regulatory action. Bob Richmond Samurai Pathologist Knoxville TN From jerry <@t> uslabsupplies.com Tue Sep 22 12:16:54 2009 From: jerry <@t> uslabsupplies.com (Jerry Helisek) Date: Tue Sep 22 12:17:13 2009 Subject: [Histonet] Re: Histonet Digest, Vol 70, Issue 27 Message-ID: <48629.94505.qm@web1112.biz.mail.sk1.yahoo.com> Laurie, I have cassettes that are 3x2x.75 in dimension. Call me to discuss how many you may need. Jerry Helisek US Laboratory Supplies, LLC 919-264-7964 jerry@uslabsupplies.com Message: 15 Date: Tue, 22 Sep 2009 08:59:40 -0700 From: "Laurie Colbert" Subject: [Histonet] Large cassettes To: Message-ID: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" My pathologist wants to sample a particular feature of a baby's brain (autopsy), but the brain is too soft to cut and still maintain this feature. She wants to process a very large section of the brain, and then cut it down after it has been processed and has some firmness to it. Does anyone know where I can get a large cassette - approximately 4" x 4" - as a sample? I don't want to have to buy a large quantity, because this is probably a one-time thing. I'm not sure such a cassette even exists, but it seems like I've seen one before. Laurie Colbert Huntington Hospital Pasadena, CA ------------------------------ From Mark.Elliott <@t> hli.ubc.ca Tue Sep 22 12:21:08 2009 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Sep 22 12:22:05 2009 Subject: [Histonet] MOM Kits In-Reply-To: <271A8BA4.973@mail.mrl.ubc.ca> References: <271A8BA4.973@mail.mrl.ubc.ca> Message-ID: <4AB8A514020000D6000431C8@mail.mrl.ubc.ca> Hi All Someone has asked me about MOM kists and since I don't do mouse work I thought I would ask the experts. What is the best MOM kit available for use with FFPE mouse tisseus? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From brett_connolly <@t> merck.com Tue Sep 22 12:26:46 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Sep 22 12:27:04 2009 Subject: [Histonet] Large cassettes In-Reply-To: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> Message-ID: <63EA0607835FBA4689CEA9EA8B482692025493F2@usctmx1141.merck.com> Laurie, Perhaps check out Electron Microscopy Sciences - autopsy capsule...a 2 part round, perforated stainless steel cassette about 3" in diameter and 0.5" deep. I have used them for processing monkey and canine brains. Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, September 22, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Large cassettes My pathologist wants to sample a particular feature of a baby's brain (autopsy), but the brain is too soft to cut and still maintain this feature. She wants to process a very large section of the brain, and then cut it down after it has been processed and has some firmness to it. Does anyone know where I can get a large cassette - approximately 4" x 4" - as a sample? I don't want to have to buy a large quantity, because this is probably a one-time thing. I'm not sure such a cassette even exists, but it seems like I've seen one before. Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tp2 <@t> medicine.wisc.edu Tue Sep 22 12:37:56 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Sep 22 12:38:15 2009 Subject: [Histonet] MOM Kits Message-ID: <4AB8C525020000DF0001C13F@gwmail.medicine.wisc.edu> Mark, I've had very good results with Biocare Medical's MOM polymer detection kits. Tom Pier >>> "Mark Elliott" 09/22/09 12:25 PM >>> Hi All Someone has asked me about MOM kists and since I don't do mouse work I thought I would ask the experts. What is the best MOM kit available for use with FFPE mouse tisseus? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SLB <@t> stowers.org Tue Sep 22 12:38:25 2009 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Tue Sep 22 12:39:31 2009 Subject: [Histonet] MOM Kits In-Reply-To: <4AB8A514020000D6000431C8@mail.mrl.ubc.ca> Message-ID: I use the MM Biotinylation Kit from Biocare and it's great as are all their products. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: Tuesday, September 22, 2009 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MOM Kits Hi All Someone has asked me about MOM kists and since I don't do mouse work I thought I would ask the experts. What is the best MOM kit available for use with FFPE mouse tisseus? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Sep 22 13:05:31 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Sep 22 13:05:36 2009 Subject: [Histonet] MOM Kits In-Reply-To: <4AB8C525020000DF0001C13F@gwmail.medicine.wisc.edu> References: <4AB8C525020000DF0001C13F@gwmail.medicine.wisc.edu> Message-ID: <622711.59159.qm@web1106.biz.mail.sk1.yahoo.com> Vector ? ________________________________ From: Thomas Pier To: Mark.Elliott@hli.ubc.ca; histonet@lists.utsouthwestern.edu Sent: Tuesday, September 22, 2009 12:37:56 PM Subject: Re: [Histonet] MOM Kits Mark, I've had very good results with Biocare Medical's MOM polymer detection kits. Tom Pier >>> "Mark Elliott" 09/22/09 12:25 PM >>> Hi All Someone has asked me about MOM kists and since I don't do mouse work I thought I would ask the experts.? What is the best MOM kit available for use with FFPE mouse tisseus? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential.? Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlbukhar <@t> ucalgary.ca Tue Sep 22 13:07:24 2009 From: mlbukhar <@t> ucalgary.ca (maureen bukhari) Date: Tue Sep 22 13:07:46 2009 Subject: [Histonet] cold trays Message-ID: <003801ca3baf$8950f420$9bf2dc60$@ca> Does anyone out there know where I can find a fridge-tray or a cold plate to cool wax blocks while cutting Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca From jqb7 <@t> cdc.gov Tue Sep 22 13:12:36 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Sep 22 13:12:58 2009 Subject: [Histonet] cold trays In-Reply-To: <003801ca3baf$8950f420$9bf2dc60$@ca> References: <003801ca3baf$8950f420$9bf2dc60$@ca> Message-ID: <0DC52B40ABF18C4884CB259763B83A5526288E@LTA3VS011.ees.hhs.gov> Sakura carries them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Tuesday, September 22, 2009 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cold trays Does anyone out there know where I can find a fridge-tray or a cold plate to cool wax blocks while cutting Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Sep 22 13:15:05 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Sep 22 13:15:09 2009 Subject: [Histonet] RE: Asbestos Body Digestion In-Reply-To: References: Message-ID: <75A0543E23D3A7458012D9E02EDBEC0005609F6F9E@UTHCMS1.uthouston.edu> Joyce Why do you want to dissolve these? I seem to remember that these are usually characteristic shaped structures that are iron positive and should be easy to identify on morphology alone or am I mistaken? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Asbestos Body Digestion Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Sep 22 13:23:34 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Sep 22 13:19:43 2009 Subject: [Histonet] cold trays In-Reply-To: <003801ca3baf$8950f420$9bf2dc60$@ca> References: <003801ca3baf$8950f420$9bf2dc60$@ca> Message-ID: <5A2BD13465E061429D6455C8D6B40E3908A115EFFF@IBMB7Exchange.digestivespecialists.com> Mercedes Medical has them. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Tuesday, September 22, 2009 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cold trays Does anyone out there know where I can find a fridge-tray or a cold plate to cool wax blocks while cutting Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Sep 22 13:24:09 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Sep 22 13:24:17 2009 Subject: [Histonet] RE: Asbestos Body Digestion In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0005609F6F9E@UTHCMS1.uthouston.edu> References: <75A0543E23D3A7458012D9E02EDBEC0005609F6F9E@UTHCMS1.uthouston.edu> Message-ID: Just trying to help a patient. I have a patient who wants to know if her lung adenoca could be tested to see if any asbestos could be involved. One of my pathologists suggested I call Mayo. Nothing there. He said someone used to do the digestion procedure at SUNY. So I asked the experts. I have a procedure from Lynette but I think I need an expert to do the testing and the resulting. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, September 22, 2009 14:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Asbestos Body Digestion Joyce Why do you want to dissolve these? I seem to remember that these are usually characteristic shaped structures that are iron positive and should be easy to identify on morphology alone or am I mistaken? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Asbestos Body Digestion Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Barry.R.Rittman <@t> uth.tmc.edu Tue Sep 22 13:27:14 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Sep 22 13:27:19 2009 Subject: [Histonet] RE: Asbestos Body Digestion In-Reply-To: References: <75A0543E23D3A7458012D9E02EDBEC0005609F6F9E@UTHCMS1.uthouston.edu> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0005609F6FAA@UTHCMS1.uthouston.edu> Joyce Off the top of my head , could you not digest tissue in a tube with proteinases, spin down and then examine any sediment? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Asbestos Body Digestion Just trying to help a patient. I have a patient who wants to know if her lung adenoca could be tested to see if any asbestos could be involved. One of my pathologists suggested I call Mayo. Nothing there. He said someone used to do the digestion procedure at SUNY. So I asked the experts. I have a procedure from Lynette but I think I need an expert to do the testing and the resulting. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, September 22, 2009 14:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Asbestos Body Digestion Joyce Why do you want to dissolve these? I seem to remember that these are usually characteristic shaped structures that are iron positive and should be easy to identify on morphology alone or am I mistaken? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Asbestos Body Digestion Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benjamin.ahlijah <@t> bioreliance.com Tue Sep 22 13:32:20 2009 From: benjamin.ahlijah <@t> bioreliance.com (Ahlijah, Benjamin) Date: Tue Sep 22 13:30:39 2009 Subject: [Histonet] Large cassettes In-Reply-To: <655216.34693.qm@web1101.biz.mail.sk1.yahoo.com> References: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> <655216.34693.qm@web1101.biz.mail.sk1.yahoo.com> Message-ID: Try Super Mega Cassettes. They are 72mmx52mmx17mm. Ben. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, September 22, 2009 12:51 PM To: Laurie Colbert; Histonet Subject: Re: [Histonet] Large cassettes Hi, I process large portions of?tissue and eyes. There are no cassettes?as large as 4"x4". Believe me I have been looking for 30 years. But, what?I use are soap molds, plastic tubs, L-blocks, etc. Paula ________________________________ From: Laurie Colbert To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 22, 2009 10:59:40 AM Subject: [Histonet] Large cassettes My pathologist wants to sample a particular feature of a baby's brain (autopsy), but the brain is too soft to cut and still maintain this feature.? She wants to process a very large section of the brain, and then cut it down after it has been processed and has some firmness to it.? Does anyone know where I can get a large cassette - approximately 4" x 4" - as a sample?? I don't want to have to buy a large quantity, because this is probably a one-time thing.? I'm not sure such a cassette even exists, but it seems like I've seen one before. Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benjamin.ahlijah <@t> bioreliance.com Tue Sep 22 13:33:50 2009 From: benjamin.ahlijah <@t> bioreliance.com (Ahlijah, Benjamin) Date: Tue Sep 22 13:32:11 2009 Subject: [Histonet] Large cassettes In-Reply-To: <655216.34693.qm@web1101.biz.mail.sk1.yahoo.com> References: <57BE698966D5C54EAE8612E8941D768306C069A0@EXCHANGE3.huntingtonhospital.com> <655216.34693.qm@web1101.biz.mail.sk1.yahoo.com> Message-ID: Super Mega cassettes are from Thermo Scientific Ben. No trees were hurt in the sending of this email however many electrons were severely inconvenienced! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, September 22, 2009 12:51 PM To: Laurie Colbert; Histonet Subject: Re: [Histonet] Large cassettes Hi, I process large portions of?tissue and eyes. There are no cassettes?as large as 4"x4". Believe me I have been looking for 30 years. But, what?I use are soap molds, plastic tubs, L-blocks, etc. Paula ________________________________ From: Laurie Colbert To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 22, 2009 10:59:40 AM Subject: [Histonet] Large cassettes My pathologist wants to sample a particular feature of a baby's brain (autopsy), but the brain is too soft to cut and still maintain this feature.? She wants to process a very large section of the brain, and then cut it down after it has been processed and has some firmness to it.? Does anyone know where I can get a large cassette - approximately 4" x 4" - as a sample?? I don't want to have to buy a large quantity, because this is probably a one-time thing.? I'm not sure such a cassette even exists, but it seems like I've seen one before. Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Sep 22 13:32:20 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Sep 22 13:32:25 2009 Subject: [Histonet] STAIN FOR NOCARDIA Message-ID: <24A4826E8EF0964D86BC5317306F58A53E3AC97345@mmc-mail.ad.mhsil.com> IN THE PAST WE HAVE USED A FITE'S STAIN FOR STAINING NOCARDIA. THE OLD PROCEDURE WE HAVE USES A XYLENE-PEANUT OIL MIXTURE OR SOLUTION. WE ARE TRYING TO COMPLETELY RID OURSELVES OF XYLENE AND TRIED THE STAIN USING A XYLENE SUBSTITUTE. WE DIDN'T HAVE MUCH LUCK. DOES ANYBODY HAVE ANY ADVICE FOR US ON THE FITES STAIN OR IS THERE ANOTHER STAIN FOR NOCARDIA THAT WOULD BE BETTER FOR US TO USE? THANKS IN ADVANCE FOR YOUR HELP. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Jackie.O'Connor <@t> abbott.com Tue Sep 22 13:36:00 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Sep 22 13:36:28 2009 Subject: [Histonet] RE: Asbestos Body Digestion In-Reply-To: References: <75A0543E23D3A7458012D9E02EDBEC0005609F6F9E@UTHCMS1.uthouston.edu> Message-ID: When I was in Honolulu, loaded with shipyards - we frequently digested lung tissue in sodium hypochlorite (bleach) to get an aggregate of asbestos fibers (if they were present). I don't recall the exact procedure, but it was quite common then. We were requested to do this by lawyers, mostly. So, I'm really not much help, just full of useless rhetoric. Hugh Luk might remember doing this? From: "Weems, Joyce" To: Date: 09/22/2009 01:29 PM Subject: RE: [Histonet] RE: Asbestos Body Digestion Sent by: histonet-bounces@lists.utsouthwestern.edu Just trying to help a patient. I have a patient who wants to know if her lung adenoca could be tested to see if any asbestos could be involved. One of my pathologists suggested I call Mayo. Nothing there. He said someone used to do the digestion procedure at SUNY. So I asked the experts. I have a procedure from Lynette but I think I need an expert to do the testing and the resulting. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, September 22, 2009 14:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Asbestos Body Digestion Joyce Why do you want to dissolve these? I seem to remember that these are usually characteristic shaped structures that are iron positive and should be easy to identify on morphology alone or am I mistaken? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Asbestos Body Digestion Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Sep 22 14:04:35 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Sep 22 14:04:46 2009 Subject: [Histonet] RE: Asbestos Body Digestion In-Reply-To: References: <75A0543E23D3A7458012D9E02EDBEC0005609F6F9E@UTHCMS1.uthouston.edu> Message-ID: Lynette Pavelich sent me a procedure. My pathologist doesn't want to do it, he thinks that somewhere in this country there is someone who does it. Lynette is checking to see if they can. Thanks to everyone! ________________________________ From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: Tuesday, September 22, 2009 14:36 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Asbestos Body Digestion When I was in Honolulu, loaded with shipyards - we frequently digested lung tissue in sodium hypochlorite (bleach) to get an aggregate of asbestos fibers (if they were present). I don't recall the exact procedure, but it was quite common then. We were requested to do this by lawyers, mostly. So, I'm really not much help, just full of useless rhetoric. Hugh Luk might remember doing this? From: "Weems, Joyce" To: Date: 09/22/2009 01:29 PM Subject: RE: [Histonet] RE: Asbestos Body Digestion Sent by: histonet-bounces@lists.utsouthwestern.edu ________________________________ Just trying to help a patient. I have a patient who wants to know if her lung adenoca could be tested to see if any asbestos could be involved. One of my pathologists suggested I call Mayo. Nothing there. He said someone used to do the digestion procedure at SUNY. So I asked the experts. I have a procedure from Lynette but I think I need an expert to do the testing and the resulting. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Rittman, Barry R Sent: Tuesday, September 22, 2009 14:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Asbestos Body Digestion Joyce Why do you want to dissolve these? I seem to remember that these are usually characteristic shaped structures that are iron positive and should be easy to identify on morphology alone or am I mistaken? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Asbestos Body Digestion Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From jengirl1014 <@t> yahoo.com Tue Sep 22 14:32:20 2009 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Tue Sep 22 14:32:25 2009 Subject: [Histonet] Antibody labeling Message-ID: <658406.92965.qm@web62301.mail.re1.yahoo.com> Hello fellow Histonetters! I was wondering if any of you have ever labeled an antibody with fluorescence.? If so which kit and protocol?did you use?? Or did you send the antibody out to be labeled?? I'm hoping that you guys can help me out yet once again!? Thank you all so much in advance.? I really appreciate all of your help. Jennifer Sipes Johns Hopkins University Baltimore, MD __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rosenfeldtek <@t> hotmail.com Tue Sep 22 14:43:37 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Sep 22 14:43:42 2009 Subject: [Histonet] Antibody labeling In-Reply-To: <658406.92965.qm@web62301.mail.re1.yahoo.com> References: <658406.92965.qm@web62301.mail.re1.yahoo.com> Message-ID: I have only done conjugation of biotin, HRP, and Alk-Phos. But I have discovered a new kit that makes the labeling process a snap. It is the "Lynx" kit from Serotec. They also have fluorophore labeling kits. It is fast, easy and very efficient. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Tue, 22 Sep 2009 12:32:20 -0700 > From: jengirl1014@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Antibody labeling > > Hello fellow Histonetters! > > I was wondering if any of you have ever labeled an antibody with fluorescence. If so which kit and protocol did you use? Or did you send the antibody out to be labeled? I'm hoping that you guys can help me out yet once again! Thank you all so much in advance. I really appreciate all of your help. > > Jennifer Sipes > Johns Hopkins University > Baltimore, MD > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Microsoft brings you a new way to search the web. Try Bing? now http://www.bing.com?form=MFEHPG&publ=WLHMTAG&crea=TEXT_MFEHPG_Core_tagline_try bing_1x1 From Robert.Lott <@t> trinitymedicalonline.com Tue Sep 22 16:57:31 2009 From: Robert.Lott <@t> trinitymedicalonline.com (Lott, Robert) Date: Tue Sep 22 16:58:31 2009 Subject: [Histonet] De-differentiated tumor vs. Radiation effect Message-ID: <4A3619571D9F6C4CB79C980E91DBE4E601549818@TNTRIEXEVS03.triadhospitals.net> Hi Everyone, One of our pathologist has some IHC results that is a bit confusing... Patient had previous Squamous cell Ca of the lung... had long courses of radiation and Chemo...eventually died... At autopsy, the tumor looks much more primitive and bizarre than before... radiation effect, etc... Are the any published papers that have looked at the effects of radiation on pre and post IHC staining.... Any help would be appreciated. Robert Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213 / 205-592-5388 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Sep 23 02:30:35 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Sep 23 02:30:49 2009 Subject: [Histonet] De-differentiated tumor vs. Radiation effect References: <4A3619571D9F6C4CB79C980E91DBE4E601549818@TNTRIEXEVS03.triadhospitals.net> Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07A1FE60@wahtntex2.waht.swest.nhs.uk> Used to report cervical cytology post radiation; many of the cells were extremely bizarre and to my mind looked malignant. Fraid I coped out and reported them as 'abnormal cells present in a post radiation smear, malignancy cannot be excluded'. These changes lasted for many years until they settled down to give a normal looking 'post menopausal smear'. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: 22 September 2009 22:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] De-differentiated tumor vs. Radiation effect Hi Everyone, One of our pathologist has some IHC results that is a bit confusing... Patient had previous Squamous cell Ca of the lung... had long courses of radiation and Chemo...eventually died... At autopsy, the tumor looks much more primitive and bizarre than before... radiation effect, etc... Are the any published papers that have looked at the effects of radiation on pre and post IHC staining.... Any help would be appreciated. Robert Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213 / 205-592-5388 ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Sep 23 05:06:44 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Sep 23 05:07:07 2009 Subject: [Histonet] cold trays In-Reply-To: <003801ca3baf$8950f420$9bf2dc60$@ca> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E137@lmhsmail.lmhealth.org> I can highly recommend the ones from Mercedes Medical. They come in two sizes. I have 3 of the smaller (red) ones and they last pretty much all day. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of maureen bukhari Sent: Tuesday, September 22, 2009 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cold trays Does anyone out there know where I can find a fridge-tray or a cold plate to cool wax blocks while cutting Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Sep 23 06:53:26 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Sep 23 06:53:28 2009 Subject: [Histonet] Re: Asbestos Body Digestion Message-ID: Joyce Weems asks about a digestion procedure for finding and roughly quantitating asbestos fibers in lung tissue. Dr. Victor Roggli at Duke performs this procedure (and interprets the results). See http://pathology.mc.duke.edu/website/WebForm.aspx?id=PulmonaryPathMain Bob Richmond Samurai Pathologist Knoxville TN From mpowers <@t> dpspa.com Wed Sep 23 09:51:09 2009 From: mpowers <@t> dpspa.com (Marian Powers) Date: Wed Sep 23 09:51:12 2009 Subject: [Histonet] P16INK4a Message-ID: <5d7de0e60909230751v53397952yd38b7a22bc7633c@mail.gmail.com> Hi; Does anyone know where I can find the P16INK4a antibody these days? ASR or IVD. Thanks, Marian From meeva <@t> med.unc.edu Wed Sep 23 10:11:11 2009 From: meeva <@t> med.unc.edu (Mervi Eeva) Date: Wed Sep 23 10:11:32 2009 Subject: [Histonet] P16INK4a In-Reply-To: <5d7de0e60909230751v53397952yd38b7a22bc7633c@mail.gmail.com> References: <5d7de0e60909230751v53397952yd38b7a22bc7633c@mail.gmail.com> Message-ID: <4ABA3A8F.1010303@med.unc.edu> http://www.mtmlabs.com/cda/products/cintecreg_histology/content-118532.html Would this help ? - Mervi UNC-CH Marian Powers wrote: > Hi; Does anyone know where I can find the P16INK4a antibody these days? > ASR or IVD. > > Thanks, > > Marian > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Mark.Elliott <@t> hli.ubc.ca Wed Sep 23 10:22:20 2009 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Sep 23 10:22:54 2009 Subject: [Histonet] Re MOM Kits In-Reply-To: References: Message-ID: <4AB9DABC.11C6.00D6.0@hli.ubc.ca> Thanks everyone for their input ion MOM kits. I have passed on the information to the person who asked me about them. Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From vapatpxs <@t> yahoo.com Wed Sep 23 11:53:19 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Sep 23 11:53:22 2009 Subject: [Histonet] RE: OT: Early Friday Hour of Fuming Message-ID: <207772.92912.qm@web46116.mail.sp1.yahoo.com> I also feel your pain. I have lab where I do electron microscopy (clinical {if they'd send me any} and research), confocal microscopy and histology. I also have added cryosectioning and will be adding a micro-CT and a deconvolution microscope (working at a BSL-2 level) with no increase in hours. I'm 80% and my lab will be shut down at the end of the year because I do not bring in enough revenue. Clinical or research-it appears there aren't enough of us and we aren't appreciated. Have the CEO of your company send out a blanket e-mail mentioning a reduction in staffing when everyone knows that I'm the only one, talk about lack of job security. Grrr, hey at least the sun is shining and there are blue skies! Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 9/21/09, Roberta Horner wrote: > From: Roberta Horner > Subject: [Histonet] RE: OT: Early Friday Hour of Fuming > To: "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" > Date: Monday, September 21, 2009, 7:36 PM > That's alright whenever I take > vacation I come back to all the work sitting here waiting > for me.? I just took 2 weeks off and it took 3 weeks to > catch up so I feel your pain. > Roberta Horner > Penn State University > Animal Diagnostic Lab > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Breeden, Sara > Sent: Monday, September 21, 2009 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT: Early Friday Hour of Fuming > > I have just found out this morning that I will be unable to > attend NSH > this year.? This alone is unfortunate news for me but, > interestingly > enough, is not due to budget issues.? The reason I am > "fuming" this > morning is not because there is no funding for my > attendance (that was > already in place), but because there is such a serious > shortage of > histotechs in this country that there is no one to cover > me!? A > "traveler" is out of the question (that's a budget issue!); > anyone that > might be hiding and is not known to me to be available in > our city would > have to have about a month's lead time to get vetted for > the position > through the State.? Anyone that thinks that histology > is not important > should try to fill a position when there is such a shortage > of trained > and qualified technicians.???If we, as > histologists, do not begin to > make ourselves more visible and promote our profession as a > worthy, > fulfilling and job-secure life, more and more of us will > find ourselves > in this position. Thank you - I feel better for having > Fumed.? There is > no need to reply as I know many of us are in the same > boat.? I was in > the boat and lost my paddle! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM? 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Wed Sep 23 12:46:44 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 23 12:46:53 2009 Subject: [Histonet] ISH on decaled bone Message-ID: We have a bone biopsy that was decaled in RDO. Of course ISH is not working. Does anyone have advice for retrieval? Many thanks. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mchavarria <@t> coreplus.com Wed Sep 23 12:52:25 2009 From: mchavarria <@t> coreplus.com (Marbella Chavarria) Date: Wed Sep 23 12:54:17 2009 Subject: [Histonet] PIN-4 Message-ID: Our lab is a specialty pathology services and one of our main area is >> prostate core biopsies which requires a lot double staining, for >> that purpose I have tried different vendors. I recently tried the >> PIN 4 > from >> Diagnostics Biosystems (dbiosys.com) and the results are excellent >> the staining is of good quality our pathologist just love it, the red >> chromogen is very stable as compare with all others I tried before. >> Finally, the price is very reasonable and the service is excellent. > I >> strongly recommend other labs to give a try to DB PIN 4 and their > other >> products. >> Regards, Marbella Chavarria BS, HTL (ASCP) Laboratory Supervisor CorePlus, LLC. 9450 S.W. 72nd Street Miami, FL 33173 Main: 305-265-8300 ext 128 Fax: 786-924-0277 E-Mail: mchavarria@coreplus.com visit us online at: www.coreplus.com CorePlus is Accredited by The Joint Commission CorePlus, LLC. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments and files transmitted with it, are confidential and are intended solely for the use of the individual(s) or entity to whom they are addressed. It may contain information that is privileged, confidential and exempt from disclosure under applicable laws. This e-mail message may also contain individually identifiable health information which is protected by The Health Insurance Portability Accountability Act of 1996 (HIPAA). If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, or if you have received this communication in error, please notify us immediately by return e-mail and delete the original message and all attachments plus any copies of it from your system immediately. If you are not the intended recipient, be advised that you have received this e-mail in error, and that any unauthorized review, use, dissemination, disclosure, distribution, forwarding, printing, or copying of any or all part(s) of the contents of this e-mail is strictly prohibited and may be illegal without our prior written permission. This communication may contain the original sender's personal views and opinions, which do not necessarily reflect those of CorePlus. From mwhite <@t> mcleodhealth.org Wed Sep 23 14:21:22 2009 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Wed Sep 23 14:21:08 2009 Subject: [Histonet] Histobath Frozen Sections Message-ID: Histonetters: In our sister lab, the Histobath freezing unit has died. We need to know if there is another comparable product available, since the Histobath is no longer manufactured. Our pathologists don't care for the peltier option. We researched the archives and found that this has been discussed on the Histonet in the past, but didn't see many responses. There is a similar (but larger, if I understand correctly) unit available from a vendor in the UK. Can anyone contribute suggestions or comments? Thanks in advance. P.S. Are there any other Samurai Pathologists available? We need one. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 From Lesley.Bechtold <@t> jax.org Wed Sep 23 15:00:48 2009 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Wed Sep 23 15:00:59 2009 Subject: [Histonet] Histology Lab Information Message-ID: <3BDA51FFD1A83B4E90829F594A5C37172CB49496FF@JAXBHMAIL01.jax.org> Dear Histonetters, We are conducting a bench-marking survey to be better informed about how other Histology Laboratories are organized and managed. We have a created a short survey in which we hope you will be willing to participate. We are primarily interested in staffing and deliverables. This is not a salary survey. Everyone who participates in our survey will receive a copy of the results (de-identified to preserve the anonymity of the participants). We hope that you will find the results useful. To complete the survey, simply click on the link below and select the "Histology Survey". You'll notice that there are multiple types of surveys listed. If you, or someone you know, works in another core facility, please feel free to complete any additional surveys that are applicable or forward them to the appropriate people. Thank you in advance for your participation! Lesley Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) http://www.jax.org/survey/index.html From abright <@t> brightinstruments.com Wed Sep 23 15:19:24 2009 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Sep 23 15:20:51 2009 Subject: [Histonet] Histobath Frozen Sections In-Reply-To: References: Message-ID: <2132620906-1253737162-cardhu_decombobulator_blackberry.rim.net-877301851-@bda177.bisx.produk.on.blackberry> Dear Melanie, There is, plus it goes down to minus 80 deg C, the Clini-RF manufactured by Bright Instrument Company , UK. Regards; Alan Bright Sent from my BlackBerry? wireless device -----Original Message----- From: mwhite@mcleodhealth.org Date: Wed, 23 Sep 2009 15:21:22 To: Subject: [Histonet] Histobath Frozen Sections Histonetters: In our sister lab, the Histobath freezing unit has died. We need to know if there is another comparable product available, since the Histobath is no longer manufactured. Our pathologists don't care for the peltier option. We researched the archives and found that this has been discussed on the Histonet in the past, but didn't see many responses. There is a similar (but larger, if I understand correctly) unit available from a vendor in the UK. Can anyone contribute suggestions or comments? Thanks in advance. P.S. Are there any other Samurai Pathologists available? We need one. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Brenda <@t> nsh.org Wed Sep 23 15:47:25 2009 From: Brenda <@t> nsh.org (Brenda Royce) Date: Wed Sep 23 15:47:30 2009 Subject: [Histonet] Looking For Roomate for NSH S/C Message-ID: Hello Histoworld - Elaine Basham is looking for a roommate for the NSH Symposium Convention. She already has a room reserved at the Sheraton and is looking for someone to share costs. She has a room from Thursday, October 1st - Thursday, October 8th, 2010. If anyone is interested, please e-mail Elaine at prtykcred@aol.com See you in Birmingham! From o.isaac24 <@t> yahoo.com Wed Sep 23 17:55:49 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Wed Sep 23 17:55:52 2009 Subject: [Histonet] IHC POSITION WANTED Message-ID: <764668.8781.qm@web111612.mail.gq1.yahoo.com> Hi, ?? I am looking for a new IHC position. I am HTL(ASCP) certified. I am open to relocation. ?Isaac. From joyceagain <@t> att.net Wed Sep 23 21:07:14 2009 From: joyceagain <@t> att.net (Joyce Hughes) Date: Wed Sep 23 21:07:18 2009 Subject: [Histonet] License Message-ID: <84950.98830.qm@web81108.mail.mud.yahoo.com> Does anyone know of a lab in Kansas City, Missouri that will help me.? I am currently going to college to get my associates degree.? I would like to become a licensed histotech.? I have found classes online through Indiana State university but I need to be working in or have access to a lab with the audio capability for televised lessons that take place every Wednesday and a histologist to be the "instructor" that will monitor my work.? ? Thanks for any help you can offer.? I am really excited about becoming a histologist but I am not really sure what direction to go.? From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Sep 24 07:22:38 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Sep 24 07:23:01 2009 Subject: [Histonet] RE: PIN-4 In-Reply-To: References: Message-ID: I have also used this system and worked it up on the N Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marbella Chavarria [mchavarria@coreplus.com] Sent: Wednesday, September 23, 2009 1:52 PM To: histonet@lists.utsouthwestern.edu Cc: Mary L. Daniel Subject: [Histonet] PIN-4 Our lab is a specialty pathology services and one of our main area is >> prostate core biopsies which requires a lot double staining, for >> that purpose I have tried different vendors. I recently tried the >> PIN 4 > from >> Diagnostics Biosystems (dbiosys.com) and the results are excellent >> the staining is of good quality our pathologist just love it, the red >> chromogen is very stable as compare with all others I tried before. >> Finally, the price is very reasonable and the service is excellent. > I >> strongly recommend other labs to give a try to DB PIN 4 and their > other >> products. >> Regards, Marbella Chavarria BS, HTL (ASCP) Laboratory Supervisor CorePlus, LLC. 9450 S.W. 72nd Street Miami, FL 33173 Main: 305-265-8300 ext 128 Fax: 786-924-0277 E-Mail: mchavarria@coreplus.com visit us online at: www.coreplus.com CorePlus is Accredited by The Joint Commission CorePlus, LLC. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments and files transmitted with it, are confidential and are intended solely for the use of the individual(s) or entity to whom they are addressed. It may contain information that is privileged, confidential and exempt from disclosure under applicable laws. This e-mail message may also contain individually identifiable health information which is protected by The Health Insurance Portability Accountability Act of 1996 (HIPAA). If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, or if you have received this communication in error, please notify us immediately by return e-mail and delete the original message and all attachments plus any copies of it from your system immediately. If you are not the intended recipient, be advised that you have received this e-mail in error, and that any unauthorized review, use, dissemination, disclosure, distribution, forwarding, printing, or copying of any or all part(s) of the contents of this e-mail is strictly prohibited and may be illegal without our prior written permission. This communication may contain the original sender's personal views and opinions, which do not necessarily reflect those of CorePlus. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Sep 24 07:30:16 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Sep 24 07:30:46 2009 Subject: [Histonet] RE: PIN-4 In-Reply-To: References: , Message-ID: Sorry email cut off. I worked it up on the Dako Autostainer Link 48. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A [Loralee_Mcmahon@URMC.Rochester.edu] Sent: Thursday, September 24, 2009 8:22 AM To: Marbella Chavarria; histonet@lists.utsouthwestern.edu Cc: Mary L. Daniel Subject: [Histonet] RE: PIN-4 I have also used this system and worked it up on the N Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marbella Chavarria [mchavarria@coreplus.com] Sent: Wednesday, September 23, 2009 1:52 PM To: histonet@lists.utsouthwestern.edu Cc: Mary L. Daniel Subject: [Histonet] PIN-4 Our lab is a specialty pathology services and one of our main area is >> prostate core biopsies which requires a lot double staining, for >> that purpose I have tried different vendors. I recently tried the >> PIN 4 > from >> Diagnostics Biosystems (dbiosys.com) and the results are excellent >> the staining is of good quality our pathologist just love it, the red >> chromogen is very stable as compare with all others I tried before. >> Finally, the price is very reasonable and the service is excellent. > I >> strongly recommend other labs to give a try to DB PIN 4 and their > other >> products. >> Regards, Marbella Chavarria BS, HTL (ASCP) Laboratory Supervisor CorePlus, LLC. 9450 S.W. 72nd Street Miami, FL 33173 Main: 305-265-8300 ext 128 Fax: 786-924-0277 E-Mail: mchavarria@coreplus.com visit us online at: www.coreplus.com CorePlus is Accredited by The Joint Commission CorePlus, LLC. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments and files transmitted with it, are confidential and are intended solely for the use of the individual(s) or entity to whom they are addressed. It may contain information that is privileged, confidential and exempt from disclosure under applicable laws. This e-mail message may also contain individually identifiable health information which is protected by The Health Insurance Portability Accountability Act of 1996 (HIPAA). If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, or if you have received this communication in error, please notify us immediately by return e-mail and delete the original message and all attachments plus any copies of it from your system immediately. If you are not the intended recipient, be advised that you have received this e-mail in error, and that any unauthorized review, use, dissemination, disclosure, distribution, forwarding, printing, or copying of any or all part(s) of the contents of this e-mail is strictly prohibited and may be illegal without our prior written permission. This communication may contain the original sender's personal views and opinions, which do not necessarily reflect those of CorePlus. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Masterson_John <@t> Allergan.com Thu Sep 24 09:10:18 2009 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Thu Sep 24 09:11:37 2009 Subject: [Histonet] Autopsy baskets wanted Message-ID: <0C58C4F16F0B67448318A38041CADE4B02992036@IRMAIL133.irvine.allergan.com> Good morning, We are looking to purchase some little metal/stainless steel baskets with expandable lids used for processing larger specimens. We just found out EMS does not sell them anymore. Does anyone here in histo-land know where we can find these? Thanks in advance, John

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation.

From relia1 <@t> earthlink.net Thu Sep 24 09:50:06 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Sep 24 09:50:11 2009 Subject: [Histonet] RELIA Histology Careers Bulletin Right Place Right Time Right Opportunity Message-ID: <23391908.1253803807151.JavaMail.root@mswamui-swiss.atl.sa.earthlink.net> Hi Histonetters!! Right Place, Right Time, Right Opportunity! Sometimes your next career move is just a matter of being in the right place at the right time and ready for the next opportunity. Odds are that if you are contemplating a job change for whatever reason ? better compensation, a more desirable location or more challenging work, you don?t have the time to do a job search. Let me help you with that. I have clients located throughout the country. All of the jobs that I represent are full time day shift positions unless otherwise noted, with excellent compensation, benefits and relocation/sign-on bonuses. If you are experienced or entry level, ASCP certified or eligible my clients are interested in speaking with you! I will assist you with your resume, coordination of interviews and coaching throughout the process. Remember my services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions or a position like anyone of these but in another area either way ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! HISTOLOGY/PATHOLOGY MANAGEMENT OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA ? Los Angeles ?Histology Supervisor MA ? Boston ? Immunohistochemistry Supervisor MA ? Cape Cod Histology Supervisor HISTOTECHS AZ ? Tucson area Histotech brand new lab CA ? San Francisco Bay Area, Research Investigator (PhD) TX- Austin Histotechnician ASCP or eligible PA- Pittsburgh, HT or HTL required. NY-Orange/Rockand County NYS license req NY-Upstate NY NYS license req NY-NYC night shift NYS license req CA - Los Angeles, Histotechnician/Histotechnologist ASCP cert req MA ? North of Boston 2nd shift position Histotechnician/Histotechnologist FL- Largo part time FL lic req RESEARCH CA ? San Francisco Bay Area, Research Investigator (PhD) Remember if nothing sounds interesting on my current list you can pass it on to your friends and wait for the next one OR give me a call or shoot me an e-mail telling me what you are looking for in your next opportunity. Remember timing is everything. Thanks ? Pam 866-60-RELIA (866-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1@earthlink.net Toll Free: 866-60-RELIA (866-607-3542) From gu.lang <@t> gmx.at Thu Sep 24 10:04:56 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 24 10:05:08 2009 Subject: AW: [Histonet] ISH on decaled bone In-Reply-To: References: Message-ID: <0D89B6A74D6348B1BCEBF5076E625B19@dielangs.at> I believe there is no chance for retrieval. DNA or RNA is degraded by hydrochloric acid. You would need some glue to stick the fragments ;). Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Weems, Joyce Gesendet: Mittwoch, 23. September 2009 19:47 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] ISH on decaled bone We have a bone biopsy that was decaled in RDO. Of course ISH is not working. Does anyone have advice for retrieval? Many thanks. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ssylvest <@t> cinci.rr.com Thu Sep 24 11:45:43 2009 From: ssylvest <@t> cinci.rr.com (ssylvest@cinci.rr.com) Date: Thu Sep 24 11:45:46 2009 Subject: [Histonet] Orienting GI biopsies during embedding Message-ID: <20090924164543.PRHJP.643287.root@hrndva-web25-z01> Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylvest@cinci.rr.com (513)803-0741 From rjbuesa <@t> yahoo.com Thu Sep 24 12:11:17 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 24 12:11:25 2009 Subject: [Histonet] Orienting GI biopsies during embedding In-Reply-To: <20090924164543.PRHJP.643287.root@hrndva-web25-z01> Message-ID: <500291.35986.qm@web65713.mail.ac4.yahoo.com> There is a very fine Technical Note by I. Dimenstein in the last issue of the JOH that can help you solve this problem. Ren? J. --- On Thu, 9/24/09, ssylvest@cinci.rr.com wrote: From: ssylvest@cinci.rr.com Subject: [Histonet] Orienting GI biopsies during embedding To: "histonet@lists.utsouthwestern.edu" Date: Thursday, September 24, 2009, 12:45 PM Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylvest@cinci.rr.com (513)803-0741 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Sep 24 12:15:16 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Sep 24 12:15:20 2009 Subject: [Histonet] Re: Histobath Frozen Sections Message-ID: I've posted a number of times about the Histobath. I don't know anything new besides the stuff I've already posted. Melanie S. White, MT(ASCP) asks: >>P.S. Are there any other Samurai Pathologists available? We need one.<< Hey, find me a way to get a South Carolina medical license at the age of 70 and we'll talk! Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Thu Sep 24 12:27:10 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Sep 24 12:27:14 2009 Subject: [Histonet] Re: Orienting GI biopsies during embedding Message-ID: Sabina Sylvest at Cincinnati Children's Hospital asks: >>We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it.<< At the very least, I'd use an OptiVISOR magnifier with 3 diopter or 4 diopter lenses - I've mentioned this item on Histonet before. See www.doneganoptical.com/optivisor.php - Donegan doesn't retail, but you can get the item from Amazon. If I had one, I'd want to use a stereo dissecting microscope with 10 and 20 power magnification, but not many pathology services have them. Orienting GI biopsies is more important than most people realize. As public awareness of celiac disease increases, we're going to have to improve our handling of upper small bowel biopsy specimens. I'm thinking of examining the fixed gross specimen with a dissecting microscope, as part of the gross description embedding with magnification doing special stains routinely - PAS (for Whipple cells) and CD3 (for infiltrating T lymphocytes in the surface epithelium) Is anyone on Histonet doing any of these things? Bob Richmond Samurai Pathologist Knoxville TN From gu.lang <@t> gmx.at Thu Sep 24 12:28:05 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 24 12:28:12 2009 Subject: [Histonet] Heidelberger Pinzette Message-ID: <8E65CB18784E4A8782468C23EA49B3C4@dielangs.at> Does anybody know a distributor of the classic "Heidelberger Pinzette" heated forcep? Gudrun Lang Histolab, AKH Linz, Austria From Rcartun <@t> harthosp.org Thu Sep 24 12:38:25 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Sep 24 12:38:34 2009 Subject: [Histonet] MSI testing Message-ID: <4ABB7650.7400.0077.1@harthosp.org> How many of you that do IHC and/or PCR testing for microsatellite instability (MSI) have your requests go through a genetic counselor? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From Nikki.Wahlberg <@t> bsci.com Thu Sep 24 12:38:14 2009 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Thu Sep 24 12:38:44 2009 Subject: [Histonet] Steiner and Steiner Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD@MAPMAIL02.bsci.bossci.com> Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. From Erin.Martin <@t> ucsf.edu Thu Sep 24 12:39:46 2009 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Sep 24 12:40:47 2009 Subject: [Histonet] SOX10 Message-ID: <379A927A452F3D43A3C8705F4E67905F0CE8271200@EX05.net.ucsf.edu> Hi, Would anyone using SOX10 antibody please tell me where they buy it? If you have a procedure that you would be willing to share as well I would be very appreciative. Thank you! Erin Martin UCSF Dermatopathology From jrobertson <@t> pathologysciences.com Thu Sep 24 12:49:33 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Thu Sep 24 12:49:36 2009 Subject: [Histonet] Steiner and Steiner In-Reply-To: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD@MAPMAIL02.bsci.bossci.com> References: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD@MAPMAIL02.bsci.bossci.com> Message-ID: <518CD6920AA7154193CBE5977CD880733A8BA1@psmgsrv2.PSMG.local> Check with Newcomer Supply as they have a Modified Steiner Kit without Uranyl Nitrate. Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor Chico, CA 95926 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, September 24, 2009 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Sep 24 12:49:41 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 24 12:49:46 2009 Subject: [Histonet] MSI testing In-Reply-To: <4ABB7650.7400.0077.1@harthosp.org> References: <4ABB7650.7400.0077.1@harthosp.org> Message-ID: Not us!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, September 24, 2009 13:38 To: Histonet Subject: [Histonet] MSI testing How many of you that do IHC and/or PCR testing for microsatellite instability (MSI) have your requests go through a genetic counselor? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From MPaul <@t> Alltech.com Thu Sep 24 12:50:14 2009 From: MPaul <@t> Alltech.com (Marquisha Paul) Date: Thu Sep 24 12:50:18 2009 Subject: [Histonet] Need a NSH S/C roommate Message-ID: <421EE4FDC90A744CB1DDDDD21A1BC56E0B2B92D1@ERGO.Alltech-bio.com> I would like to share a room for the NSH Symposium/Convention in Birmingham to help save on costs. I have a room reserved at DoubleTree from Friday - Wednesday, but if you already have a room reserved and are looking for someone to share costs, I can do that too. Send me an email at mpaul@alltech.com if you are interested Thanks a lot! Marquisha Paul Research Lab Tech Alltech, Inc. Lexington, KY From jsharding <@t> wisc.edu Thu Sep 24 13:46:16 2009 From: jsharding <@t> wisc.edu (JEFFREY S HARDING) Date: Thu Sep 24 13:46:19 2009 Subject: [Histonet] CD31 Mouse Liver Staining Message-ID: <7050ac4f65505.4abb7828@wiscmail.wisc.edu> Hello, I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections. I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. My question is regarding fixation methods IMMEDIATELY after dissection. Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining? Does the exact fixation even matter that much? Everyone seems to have something different. Thanks! Jeff From Nikki.Wahlberg <@t> bsci.com Thu Sep 24 14:01:05 2009 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Thu Sep 24 14:01:12 2009 Subject: FW: [Histonet] Steiner and Steiner Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B448103400361@MAPMAIL02.bsci.bossci.com> We already use the kit from Sigma but it is the 1% Uranyl Nitrate waste that is the issue. I will check with Newcommer to see if their kit will work. Thanks you! Nikki -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, September 24, 2009 1:44 PM To: Wahlberg, Nikki Subject: RE: [Histonet] Steiner and Steiner Nikki, What if you but the Sigma kit that has the 1% solution in it already? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, September 24, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Thu Sep 24 14:33:31 2009 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Thu Sep 24 14:33:45 2009 Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig secondary antibody on rat tissue Message-ID: it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures. Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross react with rat IgG ? Or it is due to primary antibody problem - which is unlikely because the antibody has been used without a problem in other labs. From Maria.Katleba <@t> stjoe.org Thu Sep 24 14:39:30 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Sep 24 14:39:51 2009 Subject: [Histonet] MOLECULAR LAB QUESTION Message-ID: <97C02552ECB11346877D3E83CF833ABD13ED9B728E@SJSNT-SCMAIL03.stjoe.org> Two questions (if anyone can answer): (1) What tests are you doing if you in fact have a 'molecular lab'? (2) Who runs the tests...Histotech or CLS ? Thanks! Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Edith.Gonzales <@t> providence.org Thu Sep 24 14:50:12 2009 From: Edith.Gonzales <@t> providence.org (Gonzales, Edith) Date: Thu Sep 24 14:50:16 2009 Subject: [Histonet] cap survays Message-ID: To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From rjbuesa <@t> yahoo.com Thu Sep 24 14:59:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 24 15:00:02 2009 Subject: [Histonet] Steiner and Steiner In-Reply-To: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD@MAPMAIL02.bsci.bossci.com> Message-ID: <520616.30496.qm@web65712.mail.ac4.yahoo.com> Under separate cover I am sending you a procedures for modified Steiner without uranyl nitrate. Ren? J. --- On Thu, 9/24/09, Wahlberg, Nikki wrote: From: Wahlberg, Nikki Subject: [Histonet] Steiner and Steiner To: histonet@lists.utsouthwestern.edu Date: Thursday, September 24, 2009, 1:38 PM Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate?? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of.? If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE:? This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address.? Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited.? If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Sep 24 15:16:54 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Sep 24 15:17:06 2009 Subject: [Histonet] RE: cap survays In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4CF6B66388@EXMBMCB15.ucsfmedicalcenter.org> Technologists can certainly be trained to do the scoring properly but our pathologists score them and are fully involved in the evaluations, as well as reviewing challenge slides for submission. There are also sometimes interpretation questions that have to be done on-line that the pathologists have to do, for instance for Her2. You have to ask the question of who is doing the interpretation in "real life," the techs or the pathologist? The survey is supposed to be done the same way you do it in your lab in your daily work so take that as the starting point of the discussion. I think it defeats the purpose if the pathologists are not involved since it is a test of the total quality system. Are they or aren't they part of the system? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gonzales, Edith Sent: Thursday, September 24, 2009 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap survays To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Sep 24 16:40:54 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Sep 24 16:41:57 2009 Subject: [Histonet] Re: ISH on decaled bone Message-ID: Gudrun is absolutely correct. The HCl destroyed your nucleic acids. The best decalcifier for RNA or DNA is EDTA. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From TJJ <@t> stowers.org Thu Sep 24 16:52:12 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Sep 24 16:53:09 2009 Subject: [Histonet] Re: License Message-ID: Joyce, You might have some luck calling the following places: Physician's Reference Lab, Overland Park, KS - Donna Miller Shawnee Mission Medical Center, Overland Park, KS North Kansas City Hospital - N. KC, MO - Nancy Warren University of Kansas Medical Center, Kansas City, KS - Timothy Chilton Midwest Anatomic Pathology Lab, Overland Park, KS Litton Laboratories, Blue Springs, MO Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From dellav <@t> musc.edu Thu Sep 24 17:06:45 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 24 17:06:48 2009 Subject: [Histonet] B Plus fixative on bone marrow cores In-Reply-To: <4ABB7650.7400.0077.1@harthosp.org> References: <4ABB7650.7400.0077.1@harthosp.org> Message-ID: I would appreciate comments from anyone using this fixative for bone marrow cores. Specifically I am interested in learning how long you are fixing your samples in B Plus. Manufacturer recommends "at least two hours" but we are seeing artifacts that concern me that 2 hrs may be insufficient. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From AnthonyH <@t> chw.edu.au Thu Sep 24 17:40:14 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 24 17:40:26 2009 Subject: [Histonet] Orienting GI biopsies during embedding In-Reply-To: <20090924164543.PRHJP.643287.root@hrndva-web25-z01> Message-ID: I would recommend you try to embed them on edge. Even if very small, you will be surprised how many are correctly embedded Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ssylvest@cinci.rr.com Sent: Friday, 25 September 2009 2:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orienting GI biopsies during embedding Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylvest@cinci.rr.com (513)803-0741 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Thu Sep 24 18:11:00 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 24 18:11:11 2009 Subject: [Histonet] Steiner and Steiner In-Reply-To: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD@MAPMAIL02.bsci.bossci.com> Message-ID: Nikki, The sensitisation step, from my experience, improves the silver deposition on the bacteria and decreases background staining. Margeson & Chappman (1996 Use of Zinc Formalin as a Sensitizer in Silver Stains for Spirochetes J Histotechnol 19(2):135-138) substituted zinc formalin for uranyl nitrate in the Steiner techniques and found the resulting spirochete staining to be as good as or better than when uranyl nitrate was used. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Friday, 25 September 2009 3:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lpwenk <@t> sbcglobal.net Thu Sep 24 18:22:24 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Sep 24 18:22:36 2009 Subject: [Histonet] B Plus fixative on bone marrow cores In-Reply-To: Message-ID: <1072842512EE4F958AA1E6638D38A93E@HPPav2> One of my students, a few years ago, tried several different zinc formalins from different companies, on bone marrow biopsies. At 2 hours fixation, we saw a lot of smudgy, pale baby blue nuclei. At 3 hours, we were seeing nuclear detail of the usual hematoxylin blue. 4 hours was slightly better, but our pathologists thought that the slight improvement wasn't needed to make a diagnosis - they could do it just as well at 3 hours, and it saved an hour. It didn't matter which zinc formalin we used, 3 hours seemed to be the key. So we now make 3 hours as the minimum. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, September 24, 2009 6:07 PM To: Histonet Subject: [Histonet] B Plus fixative on bone marrow cores I would appreciate comments from anyone using this fixative for bone marrow cores. Specifically I am interested in learning how long you are fixing your samples in B Plus. Manufacturer recommends "at least two hours" but we are seeing artifacts that concern me that 2 hrs may be insufficient. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Sep 24 18:29:12 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 24 18:29:20 2009 Subject: [Histonet] cap survays In-Reply-To: Message-ID: Edie, What a challenge. I would strongly recommend you give it a go. I like to score fixation, processing and staining quality of slides I and my staff produce. It allows us to have control over the science of histotechnology not the pathologists (they have enough to worry about - the diagnosis, clinical demands etc). When a department runs this way, it does not take long for the pathologist to realise the invaluable resource their labs are. In fact they become quite proud of it! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gonzales, Edith Sent: Friday, 25 September 2009 5:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap survays To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Thu Sep 24 18:35:14 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 24 18:35:18 2009 Subject: [Histonet] See you at the NSH In-Reply-To: Message-ID: Hi all, Just a note to say that I will be leaving next week for Birmingham Alabama for the NSH conference. It has taken me 30 years to get to a NSH and I am really looking forward to it. I hope to see you all (I will be the old, short, bald guy with the Aussie accent) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA - ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From disbrc <@t> shands.ufl.edu Thu Sep 24 19:12:26 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Thu Sep 24 19:12:36 2009 Subject: [Histonet] Rhodanine Copper stain In-Reply-To: <33482e9300007d0e@TREND12.Shands.Local> References: <33482e9300007d0e@TREND12.Shands.Local> Message-ID: <4ABBD2AA.72AC.0059.0@shands.ufl.edu> Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP (soon to be BS, HTL ASCP :-)) From Norm.Burnham <@t> propath.com Thu Sep 24 20:26:05 2009 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Thu Sep 24 20:26:11 2009 Subject: [Histonet] Slide and Block Disposal Message-ID: <82C7248978CB50469FD6BA68EBBEFE6701BEA48B@exchange.propathlab.com> Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham From kmcneta <@t> gmail.com Thu Sep 24 21:27:55 2009 From: kmcneta <@t> gmail.com (kmcneta@gmail.com) Date: Thu Sep 24 21:27:59 2009 Subject: [Histonet] ASCP CMP continuing education credits Message-ID: <0016368e1fec18be9804745db29f@google.com> I am attempting to renew my HT ASCP certification for the first time and I was wondering if taking the Anderson Continuing Education course (Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications-they list it as providing 36 hours) would fulfill the requirement completely. I am also certified by the FL BOH and have taken HIV and Medical errors for a few points to meet the states renewal requirements. I only have a couple months until renewal is required so any information would be greatly appreciated! Thanks, M. Mcneta From lpwenk <@t> sbcglobal.net Fri Sep 25 04:01:50 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Sep 25 04:04:01 2009 Subject: [Histonet] ASCP CMP continuing education credits In-Reply-To: <0016368e1fec18be9804745db29f@google.com> Message-ID: According to the ASCP CMP booklet, as a HT you need 36 hours (points): http://ascp.org/pdf/CMPBooklet.aspx - 1 point in Safety - 2 points minimum in area of your certification (that would be Histotechnology) - Remaining points in area of specialty, management, education or other related areas of interest So it looks like you still need 1 hour in safety, and maybe, just to be on the safe side, 2 hours specifically related to histotechnology. Now, as to whether the Anderson CE course would qualify - has any histonetter used them? Has ASCP accepted them? The closest criteria that I could fit them under would be: 4. Teleconference, subscription, or online self-instructional courses-These courses are acceptable based on any of the following criteria: a. ACCME, CMLE, ACCENT, PACE credits are awarded, or b. they are offered by a professional society (including state, regional or local chapter), or c. the course is accepted by a state licensing board, or d. the course is offered through a university or college. OR 1. Formal continuing education courses-These courses may be completed through the programs/organizations listed on the chart as well as through other professional societies such as those listed under Suggested List of Providers on page 6. Courses offered by state/regional/local societies and chapters are acceptable as well as courses offered through the continuing education departments of colleges and universities. Courses offered by organizations approved by state licensing boards are also acceptable. I don't know this company/organization, so I don't know if it would be acceptable to ASCP. And ASCP doesn't want people contacting them. "Please Note: Because of the large volume of continuing education courses available, the Board of Registry will not respond to requests for approval of individual programs or courses. If the program/course meets the criteria listed, it will be accepted for CMP points. Program provider must assign points or contact hours." Keep us informed. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kmcneta@gmail.com Sent: Thursday, September 24, 2009 10:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP CMP continuing education credits I am attempting to renew my HT ASCP certification for the first time and I was wondering if taking the Anderson Continuing Education course (Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications-they list it as providing 36 hours) would fulfill the requirement completely. I am also certified by the FL BOH and have taken HIV and Medical errors for a few points to meet the states renewal requirements. I only have a couple months until renewal is required so any information would be greatly appreciated! Thanks, M. Mcneta _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Sep 25 05:32:01 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Sep 25 05:35:43 2009 Subject: [Histonet] See you at the NSH In-Reply-To: References: Message-ID: <0DC52B40ABF18C4884CB259763B83A552628BD@LTA3VS011.ees.hhs.gov> I'll be looking for you! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, September 24, 2009 7:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] See you at the NSH Hi all, Just a note to say that I will be leaving next week for Birmingham Alabama for the NSH conference. It has taken me 30 years to get to a NSH and I am really looking forward to it. I hope to see you all (I will be the old, short, bald guy with the Aussie accent) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA - ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 25 07:52:33 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 25 07:52:36 2009 Subject: [Histonet] Rhodanine Copper stain In-Reply-To: <4ABBD2AA.72AC.0059.0@shands.ufl.edu> Message-ID: <430663.34759.qm@web65710.mail.ac4.yahoo.com> The procedure calls for thicker sections because usually the amounts of copper are small, very dispersed?and are easily seen in thicker sections. On the other hand the Rhodamine copper stain is not very sensitive. I always used Timm's method which is much more sensitive. Ren? J. --- On Thu, 9/24/09, Carrie Disbrow wrote: From: Carrie Disbrow Subject: [Histonet] Rhodanine Copper stain To: histonet@lists.utsouthwestern.edu Date: Thursday, September 24, 2009, 8:12 PM Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of? tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP? (soon to be BS, HTL ASCP :-)) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Fri Sep 25 08:15:01 2009 From: renafail <@t> bellsouth.net (MILDRED M FAIL) Date: Fri Sep 25 08:15:07 2009 Subject: [Histonet] Rhodanine Copper stain In-Reply-To: <4ABBD2AA.72AC.0059.0@shands.ufl.edu> Message-ID: <789629.84035.qm@web180302.mail.gq1.yahoo.com> Yes,? copper can be successfully demonstrated in 3 micron sections using this method. ? Rena Fail --- On Thu, 9/24/09, Carrie Disbrow wrote: ? From: Carrie Disbrow Subject: [Histonet] Rhodanine Copper stain To: histonet@lists.utsouthwestern.edu Date: Thursday, September 24, 2009, 8:12 PM Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of? tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP? (soon to be BS, HTL ASCP :-)) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cornettl <@t> hotmail.com Fri Sep 25 08:56:52 2009 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Fri Sep 25 08:56:55 2009 Subject: [Histonet] Slide and Block Disposal In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE6701BEA48B@exchange.propathlab.com> References: <82C7248978CB50469FD6BA68EBBEFE6701BEA48B@exchange.propathlab.com> Message-ID: Could everyone reply to all on this one, i have some of the same questions..... Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Thu, 24 Sep 2009 20:26:05 -0500 > From: Norm.Burnham@propath.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide and Block Disposal > > Dear Histonetters, > I think everyone on the Histonet would like to hear about all the creative, > cost effective, and HIPPA-compliant ways labs can dispose of slides and > blocks that have exceeded their retention limits. > Does anyone use an outside company that disposes of glass slides and/or > paraffin blocks, or is there equipment to be rented/purchased that would > provide a solution to everyone's slide and block disposal challenge? > We would all like to hear of your innovative or not so innovative solutions > to this challenge. > Thank you in advance for your solutions. > Norm Burnham _________________________________________________________________ Bing? brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1 From j.hough <@t> sheffield.ac.uk Fri Sep 18 05:10:31 2009 From: j.hough <@t> sheffield.ac.uk (julia hough) Date: Fri Sep 25 09:02:07 2009 Subject: [Histonet] fluorescent antibodies for IHC Message-ID: <000701ca3848$41594290$c40bc7b0$@hough@shef.ac.uk> Dear Histonetters. I've been given the challenge of finding a variety of fluorochrome conjugated antibodies for IHC, however I'm having a great deal of trouble finding them. We basically want a fluorescent primary antibody that can be visualized instantly without use of a secondary etc. We would like antibodies to bind to Osteocalcin, Alkaline Phosphatase, Periostin, TRAP 5B, Cathepsin-K and N-Cadherin. If anyone has experience using fluorescent antibodies for the above or know of anyone who produces them I would be most grateful if you could let me know. Thanks Julia From j.hough <@t> sheffield.ac.uk Fri Sep 18 09:03:10 2009 From: j.hough <@t> sheffield.ac.uk (julia hough) Date: Fri Sep 25 09:02:11 2009 Subject: [Histonet] (no subject) Message-ID: <001d01ca3868$c174ec20$445ec460$@hough@shef.ac.uk> Hello. Histonetters. I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also. I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were "jelly-like" before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. Thanks Julia From kolsen <@t> dpathwi.com Mon Sep 21 17:05:00 2009 From: kolsen <@t> dpathwi.com (Kristina M. Olsen) Date: Fri Sep 25 09:02:14 2009 Subject: [Histonet] Ultra-Low Temp. Upright Freezer In-Reply-To: Message-ID: While at my previous company, we switched to all Sanyo products because we found them superior to others. They also have the best customer service, at least here in Wisconsin. Do not buy Norlake or So-Low Kris Olsen Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, September 17, 2009 8:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ultra-Low Temp. Upright Freezer Hi all! I'm hoping to get some help RE: purchasing a new ultra-low temp. upright freezer. I've tried searching Histonet, but many of the posts are a few years old, and my guess is that the products have changed a bit over the years. My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., but preferably much larger), and definitely upright (not chest) -80. Unfortunately, since this is my first foray into the world of lab freezers, I know very little about them. I have discovered that Thermo's Revco and Sanyo freezers seem to be popular. Obviously we're looking for a freezer that is as large and efficient as possible. Insulation and the freezer's compressor(s) are most important. I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) freezer seems quite nice - small exterior footprint and his sales pitch regarding the vacuum insulation (therefore denser insulation), insulated inner doors, and supposed minimized carbon footprint was quite convincing. I still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, though my guess is that their sales pitch will be just as convincing. Since you can't depend only on sales pitches, I'm depending on colleagues as well.... Does anyone have any experience with either the Sanyo or Revco freezers (or any others that they'd like to recommend)? Any and all positive and negative feedback would be greatly appreciated! I'm hoping to get comments regarding: - efficiency - how often service is needed due to failures - are the alarms sensitive enough or maybe too sensitive - how loud the freezer is when the compressor is on - etc...... As always, please remember to Reply All. Thank you in advance for your help! I look forward to hearing everyone's suggestions. Lucie UCSD lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SyedJ2 <@t> wyeth.com Fri Sep 25 08:15:21 2009 From: SyedJ2 <@t> wyeth.com (Syed Jameel ) Date: Fri Sep 25 09:02:16 2009 Subject: [Histonet] CD31 Mouse Liver Staining Message-ID: <4ABC8A29020000F80001C19A@gvl011m.gv.us.pri.wyeth.com> Hi Jeff, I have had best success using Zinc Fixative for CD31 (PECAM1) staining in mouse tissues. It is important that the tissue is trimmed thin (~4mm) for proper fixation and recommended fixation time is 24-48 hrs. The Zinc Fixative is available from BD/Pharmingen or you can prepare it. Email me if you want details on preparing it yourself. Good luck, Jameel Message: 11 Date: Thu, 24 Sep 2009 13:46:16 -0500 From: JEFFREY S HARDING Subject: [Histonet] CD31 Mouse Liver Staining To: histonet@lists.utsouthwestern.edu Message-ID: <7050ac4f65505.4abb7828@wiscmail.wisc.edu> Content-Type: text/plain; charset=us-ascii Hello, I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections. I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. My question is regarding fixation methods IMMEDIATELY after dissection. Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining? Does the exact fixation even matter that much? Everyone seems to have something different. Thanks! Jeff From SyedJ2 <@t> wyeth.com Fri Sep 25 08:29:00 2009 From: SyedJ2 <@t> wyeth.com (Syed Jameel ) Date: Fri Sep 25 09:02:18 2009 Subject: [Histonet] CD31 Mouse Liver Staining In-Reply-To: <200909250014.n8ONsGwq001415@imail-gv6.wyeth.com> References: <200909250014.n8ONsGwq001415@imail-gv6.wyeth.com> Message-ID: <4ABC8D5C020000F80001C1AF@gvl011m.gv.us.pri.wyeth.com> Hi Jeff, I have had best results using Zinc Fixative for CD31 (PECAM1) staining in mouse tissues. You can either post fix cryosections or immersion fix tissues trimmed thin (~4mm). Zinc Fixative is available from BD/Pharmingen or you can prepare it. Email me if you want details on preparing it yourself. Good luck, Jameel >>> 9/24/2009 8:14:42 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Orienting GI biopsies during embedding (Rene J Buesa) 2. Re: Histobath Frozen Sections (Robert Richmond) 3. Re: Orienting GI biopsies during embedding (Robert Richmond) 4. Heidelberger Pinzette (Gudrun Lang) 5. MSI testing (Richard Cartun) 6. Steiner and Steiner (Wahlberg, Nikki) 7. SOX10 (Martin, Erin) 8. RE: Steiner and Steiner (Jodie Robertson) 9. RE: MSI testing (Weems, Joyce) 10. Need a NSH S/C roommate (Marquisha Paul) 11. CD31 Mouse Liver Staining (JEFFREY S HARDING) 12. FW: [Histonet] Steiner and Steiner (Wahlberg, Nikki) 13. Guniea pig anti-Doublecortin & Goat-Guinea pig secondary antibody on rat tissue ( ti fei ) 14. MOLECULAR LAB QUESTION (Maria Katleba) 15. cap survays (Gonzales, Edith) 16. Re: Steiner and Steiner (Rene J Buesa) 17. RE: cap survays (Morken, Tim) 18. Re: ISH on decaled bone (Johnson, Teri) 19. Re: License (Johnson, Teri) 20. B Plus fixative on bone marrow cores (Della Speranza, Vinnie) 21. RE: Orienting GI biopsies during embedding (Tony Henwood) 22. RE: Steiner and Steiner (Tony Henwood) 23. RE: B Plus fixative on bone marrow cores (Lee & Peggy Wenk) 24. RE: cap survays (Tony Henwood) 25. See you at the NSH (Tony Henwood) 26. Rhodanine Copper stain (Carrie Disbrow) ---------------------------------------------------------------------- Message: 1 Date: Thu, 24 Sep 2009 10:11:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Orienting GI biopsies during embedding To: "histonet@lists.utsouthwestern.edu" , ssylvest@cinci.rr.com Message-ID: <500291.35986.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There is a very fine Technical Note by I. Dimenstein in the last issue of the JOH that can help you solve this problem. Ren? J. --- On Thu, 9/24/09, ssylvest@cinci.rr.com wrote: From: ssylvest@cinci.rr.com Subject: [Histonet] Orienting GI biopsies during embedding To: "histonet@lists.utsouthwestern.edu" Date: Thursday, September 24, 2009, 12:45 PM Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylvest@cinci.rr.com (513)803-0741 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 24 Sep 2009 13:15:16 -0400 From: Robert Richmond Subject: [Histonet] Re: Histobath Frozen Sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I've posted a number of times about the Histobath. I don't know anything new besides the stuff I've already posted. Melanie S. White, MT(ASCP) asks: >>P.S. Are there any other Samurai Pathologists available? We need one.<< Hey, find me a way to get a South Carolina medical license at the age of 70 and we'll talk! Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Thu, 24 Sep 2009 13:27:10 -0400 From: Robert Richmond Subject: [Histonet] Re: Orienting GI biopsies during embedding To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Sabina Sylvest at Cincinnati Children's Hospital asks: >>We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it.<< At the very least, I'd use an OptiVISOR magnifier with 3 diopter or 4 diopter lenses - I've mentioned this item on Histonet before. See www.doneganoptical.com/optivisor.php - Donegan doesn't retail, but you can get the item from Amazon. If I had one, I'd want to use a stereo dissecting microscope with 10 and 20 power magnification, but not many pathology services have them. Orienting GI biopsies is more important than most people realize. As public awareness of celiac disease increases, we're going to have to improve our handling of upper small bowel biopsy specimens. I'm thinking of examining the fixed gross specimen with a dissecting microscope, as part of the gross description embedding with magnification doing special stains routinely - PAS (for Whipple cells) and CD3 (for infiltrating T lymphocytes in the surface epithelium) Is anyone on Histonet doing any of these things? Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Thu, 24 Sep 2009 19:28:05 +0200 From: "Gudrun Lang" Subject: [Histonet] Heidelberger Pinzette To: Message-ID: <8E65CB18784E4A8782468C23EA49B3C4@dielangs.at> Content-Type: text/plain; charset="us-ascii" Does anybody know a distributor of the classic "Heidelberger Pinzette" heated forcep? Gudrun Lang Histolab, AKH Linz, Austria ------------------------------ Message: 5 Date: Thu, 24 Sep 2009 13:38:25 -0400 From: "Richard Cartun" Subject: [Histonet] MSI testing To: "Histonet" Message-ID: <4ABB7650.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII How many of you that do IHC and/or PCR testing for microsatellite instability (MSI) have your requests go through a genetic counselor? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ------------------------------ Message: 6 Date: Thu, 24 Sep 2009 12:38:14 -0500 From: "Wahlberg, Nikki" Subject: [Histonet] Steiner and Steiner To: Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD@MAPMAIL02.bsci.bossci.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. ------------------------------ Message: 7 Date: Thu, 24 Sep 2009 10:39:46 -0700 From: "Martin, Erin" Subject: [Histonet] SOX10 To: histonet Message-ID: <379A927A452F3D43A3C8705F4E67905F0CE8271200@EX05.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" Hi, Would anyone using SOX10 antibody please tell me where they buy it? If you have a procedure that you would be willing to share as well I would be very appreciative. Thank you! Erin Martin UCSF Dermatopathology ------------------------------ Message: 8 Date: Thu, 24 Sep 2009 10:49:33 -0700 From: "Jodie Robertson" Subject: RE: [Histonet] Steiner and Steiner To: "Wahlberg, Nikki" , Message-ID: <518CD6920AA7154193CBE5977CD880733A8BA1@psmgsrv2.PSMG.local> Content-Type: text/plain; charset="us-ascii" Check with Newcomer Supply as they have a Modified Steiner Kit without Uranyl Nitrate. Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor Chico, CA 95926 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, September 24, 2009 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 24 Sep 2009 13:49:41 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] MSI testing To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Not us!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, September 24, 2009 13:38 To: Histonet Subject: [Histonet] MSI testing How many of you that do IHC and/or PCR testing for microsatellite instability (MSI) have your requests go through a genetic counselor? Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 10 Date: Thu, 24 Sep 2009 13:50:14 -0400 From: "Marquisha Paul" Subject: [Histonet] Need a NSH S/C roommate To: Message-ID: <421EE4FDC90A744CB1DDDDD21A1BC56E0B2B92D1@ERGO.Alltech-bio.com> Content-Type: text/plain; charset="us-ascii" I would like to share a room for the NSH Symposium/Convention in Birmingham to help save on costs. I have a room reserved at DoubleTree from Friday - Wednesday, but if you already have a room reserved and are looking for someone to share costs, I can do that too. Send me an email at mpaul@alltech.com if you are interested Thanks a lot! Marquisha Paul Research Lab Tech Alltech, Inc. Lexington, KY ------------------------------ Message: 11 Date: Thu, 24 Sep 2009 13:46:16 -0500 From: JEFFREY S HARDING Subject: [Histonet] CD31 Mouse Liver Staining To: histonet@lists.utsouthwestern.edu Message-ID: <7050ac4f65505.4abb7828@wiscmail.wisc.edu> Content-Type: text/plain; charset=us-ascii Hello, I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections. I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. My question is regarding fixation methods IMMEDIATELY after dissection. Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining? Does the exact fixation even matter that much? Everyone seems to have something different. Thanks! Jeff ------------------------------ Message: 12 Date: Thu, 24 Sep 2009 14:01:05 -0500 From: "Wahlberg, Nikki" Subject: FW: [Histonet] Steiner and Steiner To: Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B448103400361@MAPMAIL02.bsci.bossci.com> Content-Type: text/plain; charset="us-ascii" We already use the kit from Sigma but it is the 1% Uranyl Nitrate waste that is the issue. I will check with Newcommer to see if their kit will work. Thanks you! Nikki -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, September 24, 2009 1:44 PM To: Wahlberg, Nikki Subject: RE: [Histonet] Steiner and Steiner Nikki, What if you but the Sigma kit that has the 1% solution in it already? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, September 24, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 25 Sep 2009 03:33:31 +0800 From: " ti fei " Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig secondary antibody on rat tissue To: " Histonet " Message-ID: Content-Type: text/plain; charset="ISO-8859-1" it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures. Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross react with rat IgG ? Or it is due to primary antibody problem - which is unlikely because the antibody has been used without a problem in other labs. ------------------------------ Message: 14 Date: Thu, 24 Sep 2009 12:39:30 -0700 From: Maria Katleba Subject: [Histonet] MOLECULAR LAB QUESTION To: "histonet@lists.utsouthwestern.edu" Message-ID: <97C02552ECB11346877D3E83CF833ABD13ED9B728E@SJSNT-SCMAIL03.stjoe.org> Content-Type: text/plain; charset="us-ascii" Two questions (if anyone can answer): (1) What tests are you doing if you in fact have a 'molecular lab'? (2) Who runs the tests...Histotech or CLS ? Thanks! Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 15 Date: Thu, 24 Sep 2009 11:50:12 -0800 From: "Gonzales, Edith" Subject: [Histonet] cap survays To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 16 Date: Thu, 24 Sep 2009 12:59:58 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Steiner and Steiner To: histonet@lists.utsouthwestern.edu, NikkiWahlberg Message-ID: <520616.30496.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending you a procedures for modified Steiner without uranyl nitrate. Ren? J. --- On Thu, 9/24/09, Wahlberg, Nikki wrote: From: Wahlberg, Nikki Subject: [Histonet] Steiner and Steiner To: histonet@lists.utsouthwestern.edu Date: Thursday, September 24, 2009, 1:38 PM Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 24 Sep 2009 13:16:54 -0700 From: "Morken, Tim" Subject: [Histonet] RE: cap survays To: "Gonzales, Edith" , "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF6B66388@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Technologists can certainly be trained to do the scoring properly but our pathologists score them and are fully involved in the evaluations, as well as reviewing challenge slides for submission. There are also sometimes interpretation questions that have to be done on-line that the pathologists have to do, for instance for Her2. You have to ask the question of who is doing the interpretation in "real life," the techs or the pathologist? The survey is supposed to be done the same way you do it in your lab in your daily work so take that as the starting point of the discussion. I think it defeats the purpose if the pathologists are not involved since it is a test of the total quality system. Are they or aren't they part of the system? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gonzales, Edith Sent: Thursday, September 24, 2009 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap survays To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 24 Sep 2009 16:40:54 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: ISH on decaled bone To: histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Gudrun is absolutely correct. The HCl destroyed your nucleic acids. The best decalcifier for RNA or DNA is EDTA. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ------------------------------ Message: 19 Date: Thu, 24 Sep 2009 16:52:12 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: License To: histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Joyce, You might have some luck calling the following places: Physician's Reference Lab, Overland Park, KS - Donna Miller Shawnee Mission Medical Center, Overland Park, KS North Kansas City Hospital - N. KC, MO - Nancy Warren University of Kansas Medical Center, Kansas City, KS - Timothy Chilton Midwest Anatomic Pathology Lab, Overland Park, KS Litton Laboratories, Blue Springs, MO Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ------------------------------ Message: 20 Date: Thu, 24 Sep 2009 18:06:45 -0400 From: "Della Speranza, Vinnie" Subject: [Histonet] B Plus fixative on bone marrow cores To: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" I would appreciate comments from anyone using this fixative for bone marrow cores. Specifically I am interested in learning how long you are fixing your samples in B Plus. Manufacturer recommends "at least two hours" but we are seeing artifacts that concern me that 2 hrs may be insufficient. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 ------------------------------ Message: 21 Date: Fri, 25 Sep 2009 08:40:14 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Orienting GI biopsies during embedding To: , Message-ID: Content-Type: text/plain; charset="us-ascii" I would recommend you try to embed them on edge. Even if very small, you will be surprised how many are correctly embedded Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ssylvest@cinci.rr.com Sent: Friday, 25 September 2009 2:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orienting GI biopsies during embedding Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylvest@cinci.rr.com (513)803-0741 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 22 Date: Fri, 25 Sep 2009 09:11:00 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Steiner and Steiner To: "Wahlberg, Nikki" , Message-ID: Content-Type: text/plain; charset="us-ascii" Nikki, The sensitisation step, from my experience, improves the silver deposition on the bacteria and decreases background staining. Margeson & Chappman (1996 Use of Zinc Formalin as a Sensitizer in Silver Stains for Spirochetes J Histotechnol 19(2):135-138) substituted zinc formalin for uranyl nitrate in the Steiner techniques and found the resulting spirochete staining to be as good as or better than when uranyl nitrate was used. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Friday, 25 September 2009 3:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner Does anyone have a protocol for the Steiner and Steiner stain that does not use Uranyl Nitrate? Our EHS department will not allow us to order this chemical anymore because it is too expensive to dispose of. If anyone has any methods of disposal that are not too pricey I would be interested in them as well. Thank you very much, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 23 Date: Thu, 24 Sep 2009 19:22:24 -0400 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] B Plus fixative on bone marrow cores To: "'Della Speranza, Vinnie'" , "'Histonet'" Message-ID: <1072842512EE4F958AA1E6638D38A93E@HPPav2> Content-Type: text/plain; charset="us-ascii" One of my students, a few years ago, tried several different zinc formalins from different companies, on bone marrow biopsies. At 2 hours fixation, we saw a lot of smudgy, pale baby blue nuclei. At 3 hours, we were seeing nuclear detail of the usual hematoxylin blue. 4 hours was slightly better, but our pathologists thought that the slight improvement wasn't needed to make a diagnosis - they could do it just as well at 3 hours, and it saved an hour. It didn't matter which zinc formalin we used, 3 hours seemed to be the key. So we now make 3 hours as the minimum. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, September 24, 2009 6:07 PM To: Histonet Subject: [Histonet] B Plus fixative on bone marrow cores I would appreciate comments from anyone using this fixative for bone marrow cores. Specifically I am interested in learning how long you are fixing your samples in B Plus. Manufacturer recommends "at least two hours" but we are seeing artifacts that concern me that 2 hrs may be insufficient. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Fri, 25 Sep 2009 09:29:12 +1000 From: "Tony Henwood" Subject: RE: [Histonet] cap survays To: "Gonzales, Edith" , Message-ID: Content-Type: text/plain; charset="us-ascii" Edie, What a challenge. I would strongly recommend you give it a go. I like to score fixation, processing and staining quality of slides I and my staff produce. It allows us to have control over the science of histotechnology not the pathologists (they have enough to worry about - the diagnosis, clinical demands etc). When a department runs this way, it does not take long for the pathologist to realise the invaluable resource their labs are. In fact they become quite proud of it! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gonzales, Edith Sent: Friday, 25 September 2009 5:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap survays To all, I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis. Our paths want us to score then and they not be involved. Is this what everone else is doing? Edie DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 25 Date: Fri, 25 Sep 2009 09:35:14 +1000 From: "Tony Henwood" Subject: [Histonet] See you at the NSH To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Just a note to say that I will be leaving next week for Birmingham Alabama for the NSH conference. It has taken me 30 years to get to a NSH and I am really looking forward to it. I hope to see you all (I will be the old, short, bald guy with the Aussie accent) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA - ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 26 Date: Thu, 24 Sep 2009 20:12:26 -0400 From: "Carrie Disbrow" Subject: [Histonet] Rhodanine Copper stain To: Message-ID: <4ABBD2AA.72AC.0059.0@shands.ufl.edu> Content-Type: text/plain; charset=US-ASCII Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP (soon to be BS, HTL ASCP :-)) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 70, Issue 31 **************************************** From Lynne.Bell <@t> cvmc.org Fri Sep 25 09:09:12 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Sep 25 09:09:17 2009 Subject: [Histonet] Slide and Block Disposal In-Reply-To: Message-ID: We do the following for slide and block disposal: Slides are discarded into a rigid, puncture-proof biohazard sharps container and discarded with the regular trash. All paraffin blocks are discarded into the regular trash, as well. I believe I once read that "Joe the Toe" Nocito remarked that paraffin blocks are not hazardous (infectious) and to prove his point, he licked the block! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, VT 05641 802-371-4923 From Maria.Katleba <@t> stjoe.org Fri Sep 25 09:10:06 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Sep 25 09:10:23 2009 Subject: [Histonet] Slide and Block Disposal In-Reply-To: References: <82C7248978CB50469FD6BA68EBBEFE6701BEA48B@exchange.propathlab.com> Message-ID: <97C02552ECB11346877D3E83CF833ABD13ED9B7543@SJSNT-SCMAIL03.stjoe.org> We use SAFETY KLEEN... they are inexpensive and have the HIPPA thing down pat. In fact, they also take care of all our waste..... They truly are the best company I have ever used, and Dan goes out of his way to help his clients!!! Contact info: DAN WIENHOLZ Manager 707-584-0415 WWW.Safety-Kleen.com Maria Katleba HT (ASCP) MS Queen of the Valley Medical Center Napa CA 707-257-4076 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lorraine Cornett Sent: Friday, September 25, 2009 6:57 AM To: norm.burnham@propath.com; Histonet Listserve Subject: RE: [Histonet] Slide and Block Disposal Could everyone reply to all on this one, i have some of the same questions..... Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Thu, 24 Sep 2009 20:26:05 -0500 > From: Norm.Burnham@propath.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide and Block Disposal > > Dear Histonetters, > I think everyone on the Histonet would like to hear about all the creative, > cost effective, and HIPPA-compliant ways labs can dispose of slides and > blocks that have exceeded their retention limits. > Does anyone use an outside company that disposes of glass slides and/or > paraffin blocks, or is there equipment to be rented/purchased that would > provide a solution to everyone's slide and block disposal challenge? > We would all like to hear of your innovative or not so innovative solutions > to this challenge. > Thank you in advance for your solutions. > Norm Burnham _________________________________________________________________ Bing(tm) brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Timothy.Morken <@t> ucsfmedctr.org Fri Sep 25 10:21:48 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Sep 25 10:22:10 2009 Subject: [Histonet] FW: Slide and Block Disposal Message-ID: <1AAF670737F193429070841C6B2ADD4CF6B66580@EXMBMCB15.ucsfmedicalcenter.org> Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham From billodonnell <@t> catholichealth.net Fri Sep 25 10:26:38 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Sep 25 10:26:54 2009 Subject: [Histonet] FW: Slide and Block Disposal In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF6B66580@EXMBMCB15.ucsfmedicalcenter.org> References: <1AAF670737F193429070841C6B2ADD4CF6B66580@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: Tim, I was just thinking of that gizmo, but couldn't remember its name. They are also available for rent so that one could do their needed volume without having to keep such a thing around. Our intitution would be a prime candidate for the renting option. Plus, they look like they would be "fun" to see work! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, September 25, 2009 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Slide and Block Disposal Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From martina_sladkova <@t> yahoo.com Fri Sep 25 10:36:20 2009 From: martina_sladkova <@t> yahoo.com (Martina Sladkova) Date: Fri Sep 25 10:36:25 2009 Subject: [Histonet] Does EDTA destroy DNA during decalcifying process? Message-ID: <852106.46936.qm@web45809.mail.sp1.yahoo.com> Hello?! ? Does anyone know whether EDTA destroys DNA during decalcifying process and whether ?it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell ?proliferation. ? Thank you very much in advance, ? Martina SLADKOVA PhD student ? LABORATOIRE DE BIOINGENIERIE ET BIOMECANIQUE OSTEO-ARTICULAIRES?(B2OA) CNRS UMR 7052 Facult? de m?decine Lariboisi?re Saint-Louis Universit? Paris 7 10 avenue de Verdun 75010 PARIS T?l.: 0033 01 57 27 86 84 Fax?: 0033 01 57 27 85 71 Email: martina_sladkova@yahoo.com ? ? From mark <@t> vyleater.com Fri Sep 25 10:48:40 2009 From: mark <@t> vyleater.com (Mark J. Griffith) Date: Fri Sep 25 10:48:17 2009 Subject: [Histonet] FW: Slide and Block Disposal Message-ID: <20090925154814.8D2B218053@az.smtp.onyourmark.com> Anyone in interested in SlydEater information can contact me directly at 800-233-3271. Also, we will have a SlydEater on display in the Creative Waste Solutions booth (#839) at the upcoming NSH meeting in Birmingham. Mark Griffith Vyleater/SlydEater Product Manager S&G Enterprises, Inc. 800-233-3721 mark@slydEater.com or mark@vyleater.com http://www.SlydEater.com >Date: Fri, 25 Sep 2009 09:26:38 -0600 >From: "O'Donnell, Bill" >To: "Morken, Tim" , > histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] FW: Slide and Block Disposal >Cc: > > >Tim, > >I was just thinking of that gizmo, but couldn't remember its name. They >are also available for rent so that one could do their needed volume >without having to keep such a thing around. Our intitution would be a >prime candidate for the renting option. Plus, they look like they would >be "fun" to see work! > >William (Bill) O'Donnell, HT (ASCP) QIHC >Lead Histologist >Good Samaritan Hospital >10 East 31st Street >Kearney, NE 68847 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, >Tim >Sent: Friday, September 25, 2009 10:22 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FW: Slide and Block Disposal > > >Norm, if someone is really serious about destroying their slides, they >could check out the SlydeEater. I haven't seen it for real but it >appears to do an impressive job! > >http://www.ramflat.com/slydeater.html > >Tim Morken >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norm >Burnham >Sent: Thursday, September 24, 2009 6:26 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Slide and Block Disposal > >Dear Histonetters, >I think everyone on the Histonet would like to hear about all the >creative, cost effective, and HIPPA-compliant ways labs can dispose of >slides and blocks that have exceeded their retention limits. >Does anyone use an outside company that disposes of glass slides and/or >paraffin blocks, or is there equipment to be rented/purchased that would >provide a solution to everyone's slide and block disposal challenge? >We would all like to hear of your innovative or not so innovative >solutions to this challenge. >Thank you in advance for your solutions. >Norm Burnham > From rjbuesa <@t> yahoo.com Fri Sep 25 10:48:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 25 10:48:59 2009 Subject: [Histonet] Does EDTA destroy DNA during decalcifying process? In-Reply-To: <852106.46936.qm@web45809.mail.sp1.yahoo.com> Message-ID: <662290.71433.qm@web65711.mail.ac4.yahoo.com> Any decalcifying process,?either acid or chelating, should always?be conducted after the subject if fixed. Ren? J. --- On Fri, 9/25/09, Martina Sladkova wrote: From: Martina Sladkova Subject: [Histonet] Does EDTA destroy DNA during decalcifying process? To: histonet@lists.utsouthwestern.edu Date: Friday, September 25, 2009, 11:36 AM Hello?! ? Does anyone know whether EDTA destroys DNA during decalcifying process and whether ?it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell ?proliferation. ? Thank you very much in advance, ? Martina SLADKOVA PhD student ? LABORATOIRE DE BIOINGENIERIE ET BIOMECANIQUE OSTEO-ARTICULAIRES?(B2OA) CNRS UMR 7052 Facult? de m?decine Lariboisi?re Saint-Louis Universit? Paris 7 10 avenue de Verdun 75010 PARIS T?l.: 0033 01 57 27 86 84 Fax?: 0033 01 57 27 85 71 Email: martina_sladkova@yahoo.com ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpastor1 <@t> nycap.rr.com Fri Sep 25 11:04:11 2009 From: jpastor1 <@t> nycap.rr.com (jpastor1@nycap.rr.com) Date: Fri Sep 25 11:04:15 2009 Subject: [Histonet] Paraform cassettes Message-ID: <20090925160411.0B67J.85903.root@cdptpa-web11-z02> Has anyone had any experience using paraform cassettes? From ruebenjcarter <@t> gmail.com Fri Sep 25 11:44:02 2009 From: ruebenjcarter <@t> gmail.com (R C) Date: Fri Sep 25 11:44:05 2009 Subject: [Histonet] Dako refursbishing in Southern California Message-ID: <2a926e3f0909250944i6cae97f5hce986b55137a4bcb@mail.gmail.com> Hi all. Does anyone know of a company that will service/refurbish a Dako IHC stainer? I've tried contacting IMEB but cannot obtain a response despite several messages. Any help would be appreciated. Thanks. From integrated.histo <@t> gmail.com Fri Sep 25 11:58:16 2009 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Fri Sep 25 11:58:20 2009 Subject: [Histonet] Slide & Block disposal Message-ID: <5d9104a30909250958o182bff83ifc6e296de70022f@mail.gmail.com> Block disposal is easy for us. We dump 1 box of blocks each week or so right into the tubs we use for tissue disposal. Then the biohazard people haul it away. As for slides: We use biohazard sharps containers and have that hauled away with the other biohazardous materials. We just dump a small amount at a time so we don't have a huge disposal bill all at once. Cindy From jseaton <@t> wlgore.com Fri Sep 25 12:02:40 2009 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Fri Sep 25 12:02:47 2009 Subject: [Histonet] Janella Seaton/WLGORE is out of the office. Message-ID: I will be out of the office starting 09/25/2009 and will not return until 09/28/2009. I will respond to your message when I return. Any histology-related requests or questions can be sent to: histology@wlgore.com. From jerry <@t> uslabsupplies.com Fri Sep 25 12:06:03 2009 From: jerry <@t> uslabsupplies.com (Jerry Helisek) Date: Fri Sep 25 12:06:07 2009 Subject: [Histonet] Dako refursbishing in Southern California In-Reply-To: References: <2a926e3f0909250944i6cae97f5hce986b55137a4bcb@mail.gmail.com> Message-ID: <570107.18607.qm@web1114.biz.mail.sk1.yahoo.com> Rueben, Try Pacific Southwest Lab Equipment Larry Fox 2384 La Mirada Drive Vista, CA 92081 (760) 295-1842 larry@psl-equip.com They should be able to help you. Jerry Helisek US Laboratory Supplies, LLC 919-264-7964 jerry@uslabsupplies.com > Date: Fri, 25 Sep 2009 09:44:02 -0700 > From: ruebenjcarter@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dako refursbishing in Southern California > > Hi all. Does anyone know of a company that will service/refurbish a Dako IHC > stainer? I've tried contacting IMEB but cannot obtain a response despite > several messages. Any help would be appreciated. > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Insert movie times and more without leaving Hotmail?. See how. From Maria.Katleba <@t> stjoe.org Fri Sep 25 12:48:58 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Sep 25 12:49:18 2009 Subject: [Histonet] FW: Slide and Block Disposal In-Reply-To: References: <1AAF670737F193429070841C6B2ADD4CF6B66580@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDA6FA52@SJSNT-SCMAIL03.stjoe.org> I heard a few bad things about it...(not that I have ever used it)... But when you have tissues that had prions (that nothing but heat kills), its not a good idea to crush the slides. They need to get melted or what ever is done to sterilize them first... anyone else know about this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 25, 2009 8:27 AM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Slide and Block Disposal Tim, I was just thinking of that gizmo, but couldn't remember its name. They are also available for rent so that one could do their needed volume without having to keep such a thing around. Our intitution would be a prime candidate for the renting option. Plus, they look like they would be "fun" to see work! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, September 25, 2009 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Slide and Block Disposal Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From newacct391924 <@t> aol.com Fri Sep 25 13:10:41 2009 From: newacct391924 <@t> aol.com (newacct391924@aol.com) Date: Fri Sep 25 13:10:55 2009 Subject: [Histonet] mtm p16 protocol Message-ID: Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any help would be greatly appreciated Thank you, Carol Schultz From newacct391924 <@t> aol.com Fri Sep 25 13:17:55 2009 From: newacct391924 <@t> aol.com (newacct391924@aol.com) Date: Fri Sep 25 13:18:07 2009 Subject: [Histonet] HELP - p16 protocol Message-ID: Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any help would be greatly appreciated Thank you, Carol Schultz From jrobertson <@t> pathologysciences.com Fri Sep 25 13:58:44 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Fri Sep 25 13:58:48 2009 Subject: [Histonet] mtm p16 protocol In-Reply-To: References: Message-ID: <518CD6920AA7154193CBE5977CD880733A8BA8@psmgsrv2.PSMG.local> Unfortunately, there is no just purchasing the antibody. They package it with a negative control serum at the very least and if you wish to purchase the larger size, it comes with all the detection to perform it. I only needed the antibody and was told I couldn't purchase just that. It really increases the price when you have to purchase items that you don't need and drops the profit margin. Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor Chico, CA 95926 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of newacct391924@aol.com Sent: Friday, September 25, 2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mtm p16 protocol Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any help would be greatly appreciated Thank you, Carol Schultz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Sep 25 15:51:00 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Sep 25 15:51:04 2009 Subject: [Histonet] Re: Granular appearance to chromagen Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23D10@PHSXMB30.partners.org> Has anyone experienced a granular appearance to their chromagen pattern? I just did a run with Vector's NovaRed and all the slides show "staining" but on higher power, the appearance of the chromagen is granular (like it broke down?) I never had this problem with DAB and this is the second time I have experienced it with NovaRed. I use their kit, so after the addition of each component, I vortex to make sure it is well-mixed. Could this be the problem? Please advise. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From kalschev <@t> svm.vetmed.wisc.edu Fri Sep 25 16:55:38 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Sep 25 16:56:25 2009 Subject: [Histonet] Used Leitz 1600 Saw Microtome ? Message-ID: <00ee01ca3e2a$eb04a860$c5d76880@vetmed.wisc.edu> Please contact me. Thanks! Vicki Kalscheur Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 From gayle.callis <@t> bresnan.net Fri Sep 25 17:33:21 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Sep 25 17:33:37 2009 Subject: [Histonet] RE: EDTA and DNA damage, EDTA decalcification without fixation Message-ID: <000001ca3e30$3317a3a0$9946eae0$@callis@bresnan.net> You wrote: Does anyone know whether EDTA destroys DNA during decalcifying process and whether it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell proliferation. **************************************************************************** ******************************************************************* EDTA is the recommended decalcification per many publications on the subject especially for ISH. You need to get this publication from J Histochem Cytochem 47(5)703-309, 1999 as it may direct you to a protocol for what you are doing. Effect of Bone Decalcification Procedures on DNA In Situ Hybridization and Comparative Genomic Hybridization: EDTA Is Highly Preferable to a Routinely Used Acid Decalcifier Janneke C. Alers, Pieter-Jaap Krijtenburg, Kees J. Vissers, and Herman van Dekken Also, bone does NOT have to be fixed prior to decalcification with EDTA. I These publications used fresh calcified bone, and decalcified with EDTA using some stringent methods for performing frozen sections. J Histochem & Cytochem 51(1):5-14, 2003 http://www.jhc.org Simultaneous Detection of EGFP and Cell Surface Markers by Fluorescence Microscopy in Lymphoid Tissues Kim L. Kusser and Troy D. Randall J Histochem Cytochem S. Mori et al, 36(1)111-117, 1988 There are a large number of publications about DNA and RNA and the effects of EDTA and other decalcifiers on the nucleic acids. I did a search and brought up no less than 17 articles although did not dig through all of them. I presume you are making sections prior to extraction of DNA from EDTA treated coral? Laser Capture microdissection?? Good luck you your project. Gayle Callis HTL/HT/MT(ASCP) From gu.lang <@t> gmx.at Sat Sep 26 03:32:29 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Sep 26 03:32:42 2009 Subject: [Histonet] CISH on formic acid decalcified bonemarrow biopsies Message-ID: I agree with Gayle, that EDTA is the best way for preserving DNA and RNA. But we have switched to formic acid decal of bonemarrow biopsies for the speed-reason. And we have found that IHC has become better with some markers. This year I tested CISH (Kappa/Lambda) on 25 BM and compared the results with the IHC. With our protocol of BM processing the CISH was successful in all cases. And the results were "clearer to read". The point is, that concentration and duration of decal in formic acid must not exceeded. There are several publications on this. Our protocol: one day fixation in NBF, next day decal in 5-10% formic acid with 5-10% formaldehyde (commercial product) for 6-8 hours, then processing over night in VIP like all other specimen. These are some of the publication, I've looked up: Brown RSD, Edwards J, Bartlett JW, Jones C, Dogan A; Routine Acid Decalcification of Bone Marrow Samples Can Preserve for FISH and CGH Studies in Metastatic Prostate Cancer; J. of Histochem. and Cytochem. Vol. 50 (1): 113-115, 2002 Janneke CA, Krijtenburg P-J, Vissers KJ, van Dekken H; Effect of Bone Decalcification Procedures on DNA in Situ Hybridization and Comperative Genomic Hybridization: EDTA is Highly Preferable to a Routinely Used Acid Decalcifier, J. Histochem. and Cytochem Vol. 47(5): 703-709, 1999 Korac P, Jones M, Dominis M, Kusec R, Mason DY, Banham AH, Ventura RA; Application of the FICTION technique for the simultaneous detection of immunophenotype and chromosomal abnormalities in routinely fixed, paraffin wax embedded bone marrow trephines, J Clin Pathol 2005;58:1336-1338 Miranda RN, Mark Hon Fong L, Medeiros LJ; Fluorescent in Situ Hybridization in Routinely Processed Bone Marrow Apirate clot and Core Biopsy Sections; Am. J. of. Pathology, Vol 145, Nr. 6, December 1994 Naresh KN, Lapert I, Hasserjian R, Lykidis D, Elderfield K, Horncastle D, Smith N, Murray-Brown W, Stamp GW; Optimal processing of bones marrow trephine biopsy: the Hammersmith Protocol, J. Clin. Pathol. 2006:59;903-911 Gudrun Lang Histolab, AKH Linz, Austria From gayle.callis <@t> bresnan.net Sat Sep 26 10:33:30 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sat Sep 26 10:33:41 2009 Subject: [Histonet] CISH on formic acid decalcified bonemarrow biopsies In-Reply-To: References: Message-ID: <000901ca3ebe$b4865010$1d92f030$@callis@bresnan.net> To All, Gudrun, thank you for the references plus I liked your protocol where the BM are well fixed before decalcification. If anyone is interested I have a simple decalcification endpoint check that works as long as you have a balance that weighs in mg to three places. We use this faithfully for all bone calcification methods either acid or EDTA. It takes very little time, and certainly prevents over exposure to any acid, our preference being 10 to 15% formic acid on totally fixed murine or other species samples. Using buffered formic acid (commercial sources) is very handy for those in clinical laboratories and the buffered acid is very gentle for BM biospsies. The concentration of formic acid in sodium formate or sodium citrate buffered solutions is approximately 4.5% (I calculated this one time out of curiosity). Gayle Callis HTL,HT,MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, September 26, 2009 2:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CISH on formic acid decalcified bonemarrow biopsies I agree with Gayle, that EDTA is the best way for preserving DNA and RNA. But we have switched to formic acid decal of bonemarrow biopsies for the speed-reason. And we have found that IHC has become better with some markers. This year I tested CISH (Kappa/Lambda) on 25 BM and compared the results with the IHC. With our protocol of BM processing the CISH was successful in all cases. And the results were "clearer to read". The point is, that concentration and duration of decal in formic acid must not exceeded. There are several publications on this. Our protocol: one day fixation in NBF, next day decal in 5-10% formic acid with 5-10% formaldehyde (commercial product) for 6-8 hours, then processing over night in VIP like all other specimen. These are some of the publication, I've looked up: Brown RSD, Edwards J, Bartlett JW, Jones C, Dogan A; Routine Acid Decalcification of Bone Marrow Samples Can Preserve for FISH and CGH Studies in Metastatic Prostate Cancer; J. of Histochem. and Cytochem. Vol. 50 (1): 113-115, 2002 Janneke CA, Krijtenburg P-J, Vissers KJ, van Dekken H; Effect of Bone Decalcification Procedures on DNA in Situ Hybridization and Comperative Genomic Hybridization: EDTA is Highly Preferable to a Routinely Used Acid Decalcifier, J. Histochem. and Cytochem Vol. 47(5): 703-709, 1999 Korac P, Jones M, Dominis M, Kusec R, Mason DY, Banham AH, Ventura RA; Application of the FICTION technique for the simultaneous detection of immunophenotype and chromosomal abnormalities in routinely fixed, paraffin wax embedded bone marrow trephines, J Clin Pathol 2005;58:1336-1338 Miranda RN, Mark Hon Fong L, Medeiros LJ; Fluorescent in Situ Hybridization in Routinely Processed Bone Marrow Apirate clot and Core Biopsy Sections; Am. J. of. Pathology, Vol 145, Nr. 6, December 1994 Naresh KN, Lapert I, Hasserjian R, Lykidis D, Elderfield K, Horncastle D, Smith N, Murray-Brown W, Stamp GW; Optimal processing of bones marrow trephine biopsy: the Hammersmith Protocol, J. Clin. Pathol. 2006:59;903-911 Gudrun Lang Histolab, AKH Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From milton.gomez <@t> aruplab.com Sat Sep 26 17:33:58 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Sat Sep 26 17:34:18 2009 Subject: [Histonet] MSI Testing Message-ID: <6995F66A2196514DB0C4CAA5A32EA2931066F5@postoffice01.aruplab.net> Dr. Richard Cartun, Our laboratory offers MSI testing and typically do go thru a Genetic Counselor first. Thanks, Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.gomez@aruplab.com Web: www.aruplab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From raj <@t> bluemarble.net Sat Sep 26 18:31:28 2009 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Sat Sep 26 18:31:34 2009 Subject: [Histonet] Processors Message-ID: <2B62291D4807407A8FE740A5BEC8D4D6@CHURCH> I need advice from you wonderful techs about what processor would be best for a Derm Lab. I am going to the NSH next week and with your advice I will be able to look at some. I work now in a hospital and we have 2 VIP (Sakura) processors. I like them very much. But for a derm lab would some of the express processors be better. Thank for all your help. Becky From anonwums1 <@t> gmail.com Sat Sep 26 18:55:07 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Sat Sep 26 18:55:13 2009 Subject: [Histonet] Going from immmunohistochemistry to immunofluorescence Message-ID: <858249120909261655x63344883ie135fdd6a5c54b22@mail.gmail.com> Hi all, I have a few antibodies that I have successfully gotten to work using immunohistochemistry (primary + biotinylated secondary + SA-HRP + DAB). I am hoping to move from IHC to immunofluorescence. I tried the same staining using either a fluorescently conjugated secondary or a biotinylated secondary and an avidin conjugated fluorophore, but I don't see any staining at all. I understand the peroxidase chemistry is a lot more sensitive than fluorescence, so my question is, if I can get IHC to work, should I be able to get IF to work? If so, how would I go about troubleshooting it? How important is the flourophore (i.e. Alexa conjugates vs Texas Red vs Rhodamine). Yes, I know I could do dual immunohistochemistry, but I like fluorescence since you can see each stain independently and then merge them electronically. If anyone has a great suggestion on some chromagens that work great together, I would welcome those suggestions as well. Thanks, Adam From rjbuesa <@t> yahoo.com Sun Sep 27 07:50:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 27 07:50:47 2009 Subject: [Histonet] Processors In-Reply-To: <2B62291D4807407A8FE740A5BEC8D4D6@CHURCH> Message-ID: <545739.43168.qm@web65716.mail.ac4.yahoo.com> For any lab, not only for derm, take a look at the Skura instruments. Ren? J. --- On Sat, 9/26/09, Rebecca Johnson wrote: From: Rebecca Johnson Subject: [Histonet] Processors To: "histonet" Date: Saturday, September 26, 2009, 7:31 PM I need advice from you wonderful techs about what processor would be best for a Derm Lab.? I am going to the NSH next week and with your advice I will be able to look at some. I work now in a hospital and we have 2 VIP (Sakura) processors. I like them very much. But for a derm lab would some of the express processors be better.? Thank for all your help. Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Sun Sep 27 09:10:15 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Sun Sep 27 09:10:22 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 09/25/2009 and will not return until 09/29/2009. I will respond to your email on Tuesday. Thanks! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From kmerriam2003 <@t> yahoo.com Mon Sep 28 06:46:31 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Sep 28 06:46:37 2009 Subject: [Histonet] IHC on plasma cells Message-ID: <885986.6716.qm@web50306.mail.re2.yahoo.com> Hi everyone, I have been asked to do IHC on plasma cells of FFPE human tissue.? Anyone have?a good antibody (and method) for this procedure? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From elainemonroe <@t> live.com Mon Sep 28 07:44:47 2009 From: elainemonroe <@t> live.com (nancy monroe) Date: Mon Sep 28 07:44:51 2009 Subject: [Histonet] RE: Histonet Digest, Vol 70, Issue 35 In-Reply-To: References: Message-ID: I have used the ThermoFisher Excelsior at two different facilities. It is a wonderful processor and maintenance is a breeze, and uses less alcohol and xylene. Please take time to look at it. Elaine Monroe, Lead Histotech Spring Memorial Hospital > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 70, Issue 35 > To: histonet@lists.utsouthwestern.edu > Date: Sun, 27 Sep 2009 10:00:31 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. MSI Testing (Gomez, Milton) > 2. Processors (Rebecca Johnson) > 3. Going from immmunohistochemistry to immunofluorescence (Adam .) > 4. Re: Processors (Rene J Buesa) > 5. Laura Miller is Out of the Office. > (Laura.Miller@leica-microsystems.com) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 26 Sep 2009 16:33:58 -0600 > From: "Gomez, Milton" > Subject: [Histonet] MSI Testing > To: "Histonet" > Message-ID: > <6995F66A2196514DB0C4CAA5A32EA2931066F5@postoffice01.aruplab.net> > Content-Type: text/plain; charset="iso-8859-1" > > Dr. Richard Cartun, > > Our laboratory offers MSI testing and typically do go thru a Genetic Counselor first. > > Thanks, > > Milton A. Gomez, HTL (ASCP) > Technical Supervisor > Immunohistochemistry Department > ARUP Laboratories, Inc. > 500 Chipeta Way > Salt Lake City, UT 84108-1221 > Desk Phone: 801-583-2787, ext.3869 > Lab. Phone: 801-584-5257/5242 > Fax: 801-584-5217 > E-mail: milton.gomez@aruplab.com > Web: www.aruplab.com > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > > > ------------------------------ > > Message: 2 > Date: Sat, 26 Sep 2009 18:31:28 -0500 > From: "Rebecca Johnson" > Subject: [Histonet] Processors > To: "histonet" > Message-ID: <2B62291D4807407A8FE740A5BEC8D4D6@CHURCH> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > I need advice from you wonderful techs about what processor would be best > for a Derm Lab. I am going to the NSH next week and with your advice I will > be able to look at some. I work now in a hospital and we have 2 VIP (Sakura) > processors. I like them very much. But for a derm lab would some of the > express processors be better. Thank for all your help. > Becky > > > > > ------------------------------ > > Message: 3 > Date: Sat, 26 Sep 2009 18:55:07 -0500 > From: "Adam ." > Subject: [Histonet] Going from immmunohistochemistry to > immunofluorescence > To: histonet@lists.utsouthwestern.edu > Message-ID: > <858249120909261655x63344883ie135fdd6a5c54b22@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all, > > I have a few antibodies that I have successfully gotten to work using > immunohistochemistry (primary + biotinylated secondary + SA-HRP + DAB). I am > hoping to move from IHC to immunofluorescence. I tried the same staining > using either a fluorescently conjugated secondary or a biotinylated > secondary and an avidin conjugated fluorophore, but I don't see any staining > at all. I understand the peroxidase chemistry is a lot more sensitive than > fluorescence, so my question is, if I can get IHC to work, should I be able > to get IF to work? If so, how would I go about troubleshooting it? How > important is the flourophore (i.e. Alexa conjugates vs Texas Red vs > Rhodamine). > > Yes, I know I could do dual immunohistochemistry, but I like fluorescence > since you can see each stain independently and then merge them > electronically. If anyone has a great suggestion on some chromagens that > work great together, I would welcome those suggestions as well. > > Thanks, > Adam > > > ------------------------------ > > Message: 4 > Date: Sun, 27 Sep 2009 05:50:43 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Processors > To: histonet , Rebecca Johnson > > Message-ID: <545739.43168.qm@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > For any lab, not only for derm, take a look at the Skura instruments. > Ren? J. > > --- On Sat, 9/26/09, Rebecca Johnson wrote: > > > From: Rebecca Johnson > Subject: [Histonet] Processors > To: "histonet" > Date: Saturday, September 26, 2009, 7:31 PM > > > I need advice from you wonderful techs about what processor would be best for a Derm Lab. I am going to the NSH next week and with your advice I will be able to look at some. I work now in a hospital and we have 2 VIP (Sakura) processors. I like them very much. But for a derm lab would some of the express processors be better. Thank for all your help. > Becky > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Sun, 27 Sep 2009 09:10:15 -0500 > From: Laura.Miller@leica-microsystems.com > Subject: [Histonet] Laura Miller is Out of the Office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 09/25/2009 and will not return until > 09/29/2009. > > I will respond to your email on Tuesday. Thanks! > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 70, Issue 35 > **************************************** _________________________________________________________________ Microsoft brings you a new way to search the web. Try Bing? now http://www.bing.com?form=MFEHPG&publ=WLHMTAG&crea=TEXT_MFEHPG_Core_tagline_try bing_1x1 From mchavarria <@t> coreplus.com Mon Sep 28 08:03:41 2009 From: mchavarria <@t> coreplus.com (Marbella Chavarria) Date: Mon Sep 28 08:06:16 2009 Subject: [Histonet] ASCP CMP continuing education credits References: Message-ID: ASCP accept AndersonCE ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lee & Peggy Wenk Sent: Fri 9/25/2009 5:01 AM To: kmcneta@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP CMP continuing education credits According to the ASCP CMP booklet, as a HT you need 36 hours (points): http://ascp.org/pdf/CMPBooklet.aspx - 1 point in Safety - 2 points minimum in area of your certification (that would be Histotechnology) - Remaining points in area of specialty, management, education or other related areas of interest So it looks like you still need 1 hour in safety, and maybe, just to be on the safe side, 2 hours specifically related to histotechnology. Now, as to whether the Anderson CE course would qualify - has any histonetter used them? Has ASCP accepted them? The closest criteria that I could fit them under would be: 4. Teleconference, subscription, or online self-instructional courses-These courses are acceptable based on any of the following criteria: a. ACCME, CMLE, ACCENT, PACE credits are awarded, or b. they are offered by a professional society (including state, regional or local chapter), or c. the course is accepted by a state licensing board, or d. the course is offered through a university or college. OR 1. Formal continuing education courses-These courses may be completed through the programs/organizations listed on the chart as well as through other professional societies such as those listed under Suggested List of Providers on page 6. Courses offered by state/regional/local societies and chapters are acceptable as well as courses offered through the continuing education departments of colleges and universities. Courses offered by organizations approved by state licensing boards are also acceptable. I don't know this company/organization, so I don't know if it would be acceptable to ASCP. And ASCP doesn't want people contacting them. "Please Note: Because of the large volume of continuing education courses available, the Board of Registry will not respond to requests for approval of individual programs or courses. If the program/course meets the criteria listed, it will be accepted for CMP points. Program provider must assign points or contact hours." Keep us informed. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kmcneta@gmail.com Sent: Thursday, September 24, 2009 10:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP CMP continuing education credits I am attempting to renew my HT ASCP certification for the first time and I was wondering if taking the Anderson Continuing Education course (Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications-they list it as providing 36 hours) would fulfill the requirement completely. I am also certified by the FL BOH and have taken HIV and Medical errors for a few points to meet the states renewal requirements. I only have a couple months until renewal is required so any information would be greatly appreciated! Thanks, M. Mcneta _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Mon Sep 28 12:28:01 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Sep 28 12:29:05 2009 Subject: [Histonet] Re: IHC on plasma cells Message-ID: Kim, CD138 is a good marker for plasma cells. Contact Chris van der Loos for a protocol. He mentioned this at NSH in Denver 2007. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From laurie.colbert <@t> huntingtonhospital.com Mon Sep 28 12:47:53 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Sep 28 12:47:57 2009 Subject: [Histonet] ALK 1 Controls Message-ID: <57BE698966D5C54EAE8612E8941D768306C06F86@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can purchase ALK 1 controls? I cannot get them from American Master Tech. Laurie Colbert From galinadeyneko <@t> yahoo.com Mon Sep 28 13:42:08 2009 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Mon Sep 28 13:42:10 2009 Subject: [Histonet] COX staining in frosen muscles Message-ID: <385546.66522.qm@web33106.mail.mud.yahoo.com> Dear Colleagues. Please help. I have been asking to perform staining for ?COX (which is cytochrome oxidase I believe) activity on mouse fresh frozen leg muscles. I am not very ?familiar with enzyme histochemistry. I found one method (Seligman,1968) in J.Bancroft , 5 edition book, and the other in J. Kiernan "Histochemical Methods" book, but the methods are described for the fixed brain. I? am absolutely not sure that I have found the required methods. Could you share with me your experience, detailed protocol or the sources where i can find the reliable protocol siutable for muscle histochemistry.Thank you in advance. Galina Deyneko Novartis Cambridge, MA From batesf <@t> ohsu.edu Mon Sep 28 13:51:29 2009 From: batesf <@t> ohsu.edu (Florence Leomiti) Date: Mon Sep 28 13:51:35 2009 Subject: [Histonet] COX staining in frosen muscles In-Reply-To: <385546.66522.qm@web33106.mail.mud.yahoo.com> References: <385546.66522.qm@web33106.mail.mud.yahoo.com> Message-ID: <0D57C92D2BD3C24ABA05093F55D3EC050B1352@EX-BE07.ohsu.edu> Hi Galina I work in the neuromuscular lab and perform this stain on a weekly basis I have a protocol I can share with you. Just contact me and I can send it on. Florence Leomiti HT (ASCP) OHSU Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-494-6787 Pager 16822 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Monday, September 28, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] COX staining in frosen muscles Dear Colleagues. Please help. I have been asking to perform staining for ?COX (which is cytochrome oxidase I believe) activity on mouse fresh frozen leg muscles. I am not very ?familiar with enzyme histochemistry. I found one method (Seligman,1968) in J.Bancroft , 5 edition book, and the other in J. Kiernan "Histochemical Methods" book, but the methods are described for the fixed brain. I? am absolutely not sure that I have found the required methods. Could you share with me your experience, detailed protocol or the sources where i can find the reliable protocol siutable for muscle histochemistry.Thank you in advance. Galina Deyneko Novartis Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Mon Sep 28 13:57:51 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Sep 28 13:58:03 2009 Subject: [Histonet] OSCAR control Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDA6FF58@SJSNT-SCMAIL03.stjoe.org> Any ideas on what I can use to optimize OSACR antibody? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From akemiat3377 <@t> yahoo.com Mon Sep 28 14:24:49 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Sep 28 14:24:53 2009 Subject: [Histonet] OSCAR control In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13EDA6FF58@SJSNT-SCMAIL03.stjoe.org> Message-ID: <88151.85846.qm@web31303.mail.mud.yahoo.com> Dr. Allen Gown, from PhenoPath laboratories developed this antibody.? Perhaps you might contact Patti Loykasek at PhenoPath at (206) 374-9000 and she might help you. Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com --- On Mon, 9/28/09, Maria Katleba wrote: From: Maria Katleba Subject: [Histonet] OSCAR control To: "histonet@lists.utsouthwestern.edu" Date: Monday, September 28, 2009, 11:57 AM Any ideas on what I can use to optimize OSACR antibody? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Mon Sep 28 14:41:49 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Sep 28 14:41:53 2009 Subject: [Histonet] Position In NY Message-ID: Hello, I have the following position open, please let me know if anyone is interested out there. Thank you :-) TITLE/POSITION Status: Exempt. Shift: Regular, full-time, Day Shift either 5:30am start time, or 7:30am start time Histotechnologist/Histotechnician Work Shift: Monday-Friday QUALIFICATIONS ASCP certification required Prior experience working as a histotech a must Degree preferred, but not required NY license Be sure to submit your resume to alyssa@alliedsearchpartners.com for review and prescreening. *All inquiries are kept confidential* and one of our recruiters will call for an initial phone screen. Thank you! *Be sure to visit us on the web* www.alliedsearchpartners.com to submit a referral for a cash bonus, submit a job request, or contact one of our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From disbrc <@t> shands.ufl.edu Mon Sep 28 15:08:45 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Mon Sep 28 15:08:57 2009 Subject: [Histonet] Re: Responses to micron thickness for Rhodanine copper In-Reply-To: <47e0f7660008ceee@TREND12.Shands.Local> References: <47e0f7660008ceee@TREND12.Shands.Local> Message-ID: <4AC0DF8C.72AC.0059.0@shands.ufl.edu> Hi and thank you all for your responses! Everyone agreed that the thicker sections allow the copper pigments to be better visualized. However the responders were split 50/50 in the thickness. There were labs that did cut the sections at 3 microns with continuous good results and the others at 6 microns. One lab cut the sections at 10 microns. I appreciate all your help and now we will test on three microns too. Thanks again, Carrie From batesf <@t> ohsu.edu Mon Sep 28 15:14:22 2009 From: batesf <@t> ohsu.edu (Florence Leomiti) Date: Mon Sep 28 15:14:29 2009 Subject: [Histonet] COX staining in frosen muscles In-Reply-To: References: <385546.66522.qm@web33106.mail.mud.yahoo.com> <0D57C92D2BD3C24ABA05093F55D3EC050B1352@EX-BE07.ohsu.edu> Message-ID: <0D57C92D2BD3C24ABA05093F55D3EC050B1353@EX-BE07.ohsu.edu> No Problem Delores Send me your fax number and I will send over our protocol. Florence Leomiti HT (ASCP) OHSU Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-494-6787 Pager 16822 -----Original Message----- From: Diaz, Dolores [mailto:dolores.diaz@ttuhsc.edu] Sent: Monday, September 28, 2009 12:41 PM To: Florence Leomiti Subject: RE: [Histonet] COX staining in frosen muscles Hello, Florence I work in a research facility and we are beginning to stain frozen rat muscle. I will attempt to stain for COX and would also love to have a copy of your protocol if it isn't asking too much. Thank you, Dolores Diaz HT (ASCP) TTUHSC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Florence Leomiti Sent: Monday, September 28, 2009 12:51 PM To: Galina Deyneko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] COX staining in frosen muscles Hi Galina I work in the neuromuscular lab and perform this stain on a weekly basis I have a protocol I can share with you. Just contact me and I can send it on. Florence Leomiti HT (ASCP) OHSU Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-494-6787 Pager 16822 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Monday, September 28, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] COX staining in frosen muscles Dear Colleagues. Please help. I have been asking to perform staining for ?COX (which is cytochrome oxidase I believe) activity on mouse fresh frozen leg muscles. I am not very ?familiar with enzyme histochemistry. I found one method (Seligman,1968) in J.Bancroft , 5 edition book, and the other in J. Kiernan "Histochemical Methods" book, but the methods are described for the fixed brain. I? am absolutely not sure that I have found the required methods. Could you share with me your experience, detailed protocol or the sources where i can find the reliable protocol siutable for muscle histochemistry.Thank you in advance. Galina Deyneko Novartis Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Mon Sep 28 15:16:22 2009 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Mon Sep 28 15:18:38 2009 Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig secondaryantibody on rat tissue Message-ID: Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig secondaryantibody on rat tissue it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures. Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross react with rat IgG ? Or it is due to primary antibody problem - which is unlikely because the antibody has been used without a problem in other labs. From FMonson <@t> wcupa.edu Mon Sep 28 15:24:16 2009 From: FMonson <@t> wcupa.edu (Monson, Frederick) Date: Mon Sep 28 15:24:35 2009 Subject: [Histonet] Re: Responses to micron thickness for Rhodanine copper In-Reply-To: <4AC0DF8C.72AC.0059.0@shands.ufl.edu> References: <47e0f7660008ceee@TREND12.Shands.Local> <4AC0DF8C.72AC.0059.0@shands.ufl.edu> Message-ID: <86FEE8BCF5652949A08D1EB0545EB42403D45FFEEE@WCU-EX-EMP1-MB.PASSHE.LCL> Once upon a time there was an investigator who used sections of testis of unspecified thickness to stain with iron hemtoxylin. From his observations of the results, he concluded that there were two distinct populations of Sertoli cells based on his observation of two distinct populations of Sertoli cell nuclei - one darkly stained and one lightly stained. The paper was published in Scandanavia in the late 1950's. My unsupported conclusion was - I was very young at the time - that intact nuclei will hold more stain/dye than sectioned nuclei (not sealed to the slide) in a procedure that requires a 'differentiation' step with the mordant. In this case, do thicker sections hold more intact cells? Just thought I'd ask the question. Cheers, and I miss you folks more that you miss me, I bet! Fred Monson Frederick C. Monson, PhD Technical Director, CMIRT West Chester University West Chester, PA, 19383 610-738-0437 New Web Site:?? ? ????? http://cmirt.wcupa.edu/index.html New Scheduler:??? ????? http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl Reads of the Month: 1. "The Liver as a Lymphoid Organ," Ian Nicholas Crispe, Annual Review of Immunology, Apr 2009, Vol. 27: 147-163. (Access Depends on subscription.) 2. "A Personal Journey of Discovery: Developing Technology and Changing Biology," Lee Hood, Annual Review of Analytic Chemistry, Vol. 1: 1-43. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Monday, September 28, 2009 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Responses to micron thickness for Rhodanine copper Hi and thank you all for your responses! Everyone agreed that the thicker sections allow the copper pigments to be better visualized. However the responders were split 50/50 in the thickness. There were labs that did cut the sections at 3 microns with continuous good results and the others at 6 microns. One lab cut the sections at 10 microns. I appreciate all your help and now we will test on three microns too. Thanks again, Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon Sep 28 15:27:38 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Sep 28 15:27:47 2009 Subject: [Histonet] Oscar Message-ID: Hi All. I understand that there was a recent question regarding the antibody Oscar (pan cytokeratin). I've been off histonet while I was on vacation. We routinely work up Oscar using normal liver. There should be staining of the bile ducts and the hepatocytes. I'd be happy to offer more info if the person who originally posted will contact me. I'll try not to take vacation again ( at least until the next available time!). Happy Monday. Patti Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Maria.Katleba <@t> stjoe.org Mon Sep 28 15:33:48 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Sep 28 15:33:59 2009 Subject: [Histonet] Oscar In-Reply-To: References: Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDA70057@SJSNT-SCMAIL03.stjoe.org> Patti, Thank you for taking time to talk to me. I appreciate the help! Kind regards, Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 707-257-4076 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Monday, September 28, 2009 1:28 PM To: histonet Subject: [Histonet] Oscar Hi All. I understand that there was a recent question regarding the antibody Oscar (pan cytokeratin). I've been off histonet while I was on vacation. We routinely work up Oscar using normal liver. There should be staining of the bile ducts and the hepatocytes. I'd be happy to offer more info if the person who originally posted will contact me. I'll try not to take vacation again ( at least until the next available time!). Happy Monday. Patti Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From jnocito <@t> satx.rr.com Mon Sep 28 19:59:47 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Sep 28 20:00:05 2009 Subject: [Histonet] Oscar In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13EDA70057@SJSNT-SCMAIL03.stjoe.org> References: <97C02552ECB11346877D3E83CF833ABD13EDA70057@SJSNT-SCMAIL03.stjoe.org> Message-ID: Oscar? when I was the supervisor of the immuno lab at the AFIP, our rabbit that produced our anti-keratin died. The civilian researcher and I were working up multiple cytokeratin cocktails to replace Fluffy. We wanted to call it the BOZO antibody, but our medical director refused. I can see it now, in print " 50 cases were stained with the Bozo antibody", now there's Oscar. Tell me this antibody didn't come from Hollywood. JTT ----- Original Message ----- From: "Maria Katleba" To: "Patti Loykasek" ; "histonet" Sent: Monday, September 28, 2009 3:33 PM Subject: RE: [Histonet] Oscar Patti, Thank you for taking time to talk to me. I appreciate the help! Kind regards, Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 707-257-4076 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Monday, September 28, 2009 1:28 PM To: histonet Subject: [Histonet] Oscar Hi All. I understand that there was a recent question regarding the antibody Oscar (pan cytokeratin). I've been off histonet while I was on vacation. We routinely work up Oscar using normal liver. There should be staining of the bile ducts and the hepatocytes. I'd be happy to offer more info if the person who originally posted will contact me. I'll try not to take vacation again ( at least until the next available time!). Happy Monday. Patti Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From disbrc <@t> shands.ufl.edu Mon Sep 28 21:16:40 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Mon Sep 28 21:16:57 2009 Subject: [Histonet] More Copper stain thickness Message-ID: <4AC135C6.72AC.0059.0@shands.ufl.edu> Hi Histonet! Regarding the discussion about the thickness of FFPE sections for Rhodanine copper, ie 3 um vs. 6 um, someone asked me: If everyone agreed that thicker sections are better, how it can be a 50/50 split about the thickness? Isn't that an unsustainable contradiction? (and I thank them for that question too!) I was saying that yes everyone agrees that more copper will show up in thicker sections and 50 % of the respondents said that they can demonstrate copper in thin sections too however the positive staining will be a little weaker than in the 6 micron sections. I hope that answers the question. I'm the one who does not want to change our procedure until our lab can prove 3 um sections demonstrate copper. The problem is that we get unstained slides cut at 3 um and no block (consult cases). Also, someone asked if: "In this case, do thicker sections hold more intact cells?" because the " intact nuclei will hold more stain/dye than sectioned nuclei (not sealed to the slide) in a procedure that requires a 'differentiation' step with the mordant." I'm sure the answer to that is, "It depends." Thanks for all your help! Carrie From Kjones <@t> upei.ca Tue Sep 29 08:20:01 2009 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Tue Sep 29 08:20:09 2009 Subject: [Histonet] BLTS Message-ID: <4AC1DF51.BDAE.008B.1@groupwise.upei.ca> Hello Histonet I am wondering if anyone out there is familiar with the Barbeito-Lopez Trichrome stain. I am using it to identify early myocardial infarction. My damaged tissue is showing up, but it is very subtle. The papers I've read show the necrotic myofibers 'popping' out as yellow, whereas mine are more pale, almost pink. I'm not sure if I am under decolorizing or if I should leave it in the molybdic acid aniline blue methyl orange longer. Any suggestions would be greatly appreciated! Kathy Jones Research Technician AVC-UPEI From DKnutson <@t> primecare.org Tue Sep 29 08:21:55 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Sep 29 08:22:21 2009 Subject: [Histonet] Voice Recognition Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B67C5@exchangent> Hello fellow Histonetters - I could use some advice from those who have voice recognition in place at their labs. We are having an online demo given to us soon and I could use some help on any pertinent questions that should be addressed. Are there any concerns that I should be asking about? Would appreciate any pros and cons from all of you. Thank you very much in advance! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From ploykasek <@t> phenopath.com Tue Sep 29 09:04:15 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Sep 29 09:04:32 2009 Subject: [Histonet] Oscar In-Reply-To: Message-ID: Hi Joe. I had never thought of the name as related to Hollywood! Funny. Actually the name stands for Our Second Cytokeratin Antibody Rocks! A bit of path humor (or at least Dr. Gown tried to be humorous!). We are truly a bit silly sometimes. Hope everyone has a good week. We are into the rainy Fall here in Seattle. Patti Loykasek PhenoPath > Oscar? when I was the supervisor of the immuno lab at the AFIP, our rabbit > that produced our anti-keratin died. The civilian researcher and I were > working up multiple cytokeratin cocktails to replace Fluffy. We wanted to > call it the BOZO antibody, but our medical director refused. I can see it > now, in print " 50 cases were stained with the Bozo antibody", now there's > Oscar. Tell me this antibody didn't come from Hollywood. > > JTT > ----- Original Message ----- > From: "Maria Katleba" > To: "Patti Loykasek" ; "histonet" > > Sent: Monday, September 28, 2009 3:33 PM > Subject: RE: [Histonet] Oscar > > > Patti, > > Thank you for taking time to talk to me. I appreciate the help! > > Kind regards, > > Maria Katleba HT(ASCP) MS > Queen of the Valley Medical Center > Napa CA 94558 > > 707-257-4076 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti > Loykasek > Sent: Monday, September 28, 2009 1:28 PM > To: histonet > Subject: [Histonet] Oscar > > Hi All. I understand that there was a recent question regarding the antibody > Oscar (pan cytokeratin). I've been off histonet while I was on vacation. We > routinely work up Oscar using normal liver. There should be staining of the > bile ducts and the hepatocytes. I'd be happy to offer more info if the > person who originally posted will contact me. I'll try not to take vacation > again ( at least until the next available time!). Happy Monday. > > Patti Loykasek > PhenoPath Laboratories > > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended > recipient, please contact the sender by e-mail and destroy all copies of the > original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Notice from St. Joseph Health System: > Please note that the information contained in this message may be privileged > and confidential and protected from disclosure. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mwatson <@t> gnf.org Tue Sep 29 10:06:50 2009 From: mwatson <@t> gnf.org (James Watson) Date: Tue Sep 29 10:06:56 2009 Subject: [Histonet] Entry level Histology position San Diego Message-ID: Job Description GNF is currently seeking a Scientific Associate to join the Histology group Job Summary Performs routine, special staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications Associate's degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. Acquiring the American Society of Clinical Pathologist (ASCP) certification as a Histology Technician within one year will be required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. Experience An entry level technician is acceptable. We will train the employee in animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs microtomy on rotary microtome, cryostat, and be able to learn to use a sliding microtome. 4. Prepares dyes and solutions in order to perform special or complex procedures. 5. Have the background to do basic histochemical stains and enzymatic histochemical stains. Have the background to learn Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning Instrumentation. The Genomics Institute of the Novartis Research Foundation (GNF), located in the Torrey Pines area of San Diego, CA, is funded by the Novartis Research Foundation and dedicated to the development and application of new methods and techniques for genome-wide biological discovery and biomedical research. GNF provides a unique and challenging opportunity to combine exploratory biomedical research with pharmaceutical drug development in a highly interactive, multidisciplinary environment and state-of-the-art facilities. GNF offers excellent compensation and a great benefits package. Visit our website at www.gnf.org EOE Please submit your CV and any supporting documents to: Genomics Institute of the Novartis Research Foundation Job Code: JW09-025 10675 John Jay Hopkins Drive San Diego, CA 92121 Fax: 858/812-1670, or submit online to jobs@gnf.org (subject line must include JW09-025) James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From jeri <@t> opssearchgroup.com Tue Sep 29 10:16:59 2009 From: jeri <@t> opssearchgroup.com (jeri@opssearchgroup.com) Date: Tue Sep 29 10:17:27 2009 Subject: [Histonet] HT job opportunity Message-ID: <6C685E68BA5146ED910A695939670650@D3RWK391> Hello HT career seekers. Great opportunity for someone who enjoys being a part of a team and wants to demonstrate their well honed Histo skills. This is a large, well known medical facility located in Ohio. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY From mward <@t> wfubmc.edu Tue Sep 29 10:19:27 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Sep 29 10:19:32 2009 Subject: [Histonet] MUM 1 antibody Message-ID: <61135F0455D33347B5AAE209B903A30429D350FA@EXCHVS2.medctr.ad.wfubmc.edu> I have been asked by my hematopathologist to work up MUM 1 antibody. Does anyone have any suggestions as far as vendor? Any additional advice for the Bond stainer would also be appreciated. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From sweaver <@t> tvmdl.tamu.edu Tue Sep 29 10:25:13 2009 From: sweaver <@t> tvmdl.tamu.edu (Stephanie Weaver) Date: Tue Sep 29 10:25:51 2009 Subject: [Histonet] formalin neutralization Message-ID: <4AC1E089.A3DC.00A0.0@tvmdl.tamu.edu> For those who are better at chemistry than I am: I have just purchased some Formalex Green to neutralize our waste 10% formalin for safe drain disposal. I noticed that I can also purchase a "test kit" that will tell me if the aldehydes are safely neutralized. Instead of that, couldn't I just add a drop of Schiff's reagent to the final product, and that would indicate if there are any unreacted aldehydes? Thank you in advance, Stephanie Weaver Texas Veterinary Diagnostic Lab From rjbuesa <@t> yahoo.com Tue Sep 29 10:40:09 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 29 10:46:53 2009 Subject: [Histonet] formalin neutralization In-Reply-To: <4AC1E089.A3DC.00A0.0@tvmdl.tamu.edu> Message-ID: <912877.63181.qm@web65708.mail.ac4.yahoo.com> Yes you can but?prepare yourself for a surprise: you will almost always get a positive reaction! Ren? J. --- On Tue, 9/29/09, Stephanie Weaver wrote: From: Stephanie Weaver Subject: [Histonet] formalin neutralization To: "histonet post" Date: Tuesday, September 29, 2009, 11:25 AM For those who are better at chemistry than I am: I have just purchased some Formalex Green to neutralize our waste 10% formalin for safe drain disposal.? I noticed that I can also purchase a "test kit" that will tell me if the aldehydes are safely neutralized.? Instead of that, couldn't I just add a drop of Schiff's reagent to the final product, and that would indicate if there are any unreacted aldehydes? Thank you in advance, Stephanie Weaver Texas Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> smdc.org Tue Sep 29 10:53:12 2009 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Tue Sep 29 10:53:55 2009 Subject: [Histonet] cytology core biopsy billing question Message-ID: I am wondering how cytology core biopsies are billed in your facility. We accession our core biopsies as surgical specimens, and are having a lively discussion amongst our pathologist about how to bill for the adequacy for these specimens. Can we bill an 88329, Intraoperative Consultation for the adequacy the pathologist is performing? We do charge an 88333 for the touch preps - but the docs are split on whether it is appropriate to also bill the 88329 as well. Thanks for any information you may offer! Sheila Tapper Anatomic Pathology Supervisor SMDC Laboratory 218-786-5472 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From sfeher <@t> CMC-NH.ORG Tue Sep 29 10:56:30 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Tue Sep 29 11:00:36 2009 Subject: [Histonet] Voice Recognition In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B67C5@exchangent> References: <4F0B7161A6CD524FAD8017D52E1553400D2B67C5@exchangent> Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1FC3@exchange.cmc-nh.org> We are getting set to launch a Dragon system with a Voice Brooke interface at our lab. One of the questions that slipped by us when we were looking into the various systems was with synoptic reporting. Make sure the system you are looking into will interface with your software LIS to allow synoptic reporting by voice rather than by having to use the computer mouse to select the appropriate synoptic response. Most everything else interfaces very well, including voice commands to acquire images taken with a digital camera. Good Luck, Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Tuesday, September 29, 2009 9:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Voice Recognition Hello fellow Histonetters - I could use some advice from those who have voice recognition in place at their labs. We are having an online demo given to us soon and I could use some help on any pertinent questions that should be addressed. Are there any concerns that I should be asking about? Would appreciate any pros and cons from all of you. Thank you very much in advance! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Sep 29 11:27:23 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Sep 29 11:27:29 2009 Subject: [Histonet] formalin neutralization In-Reply-To: <4AC1E089.A3DC.00A0.0@tvmdl.tamu.edu> References: <4AC1E089.A3DC.00A0.0@tvmdl.tamu.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38D785D7@LRGHEXVS1.practice.lrgh.org> What does your state require? New Hampshire requires to test with the test kit and if at zero we can drain dispose. The state also requires us to keep our test records for five years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Tuesday, September 29, 2009 11:25 AM To: histonet post Subject: [Histonet] formalin neutralization For those who are better at chemistry than I am: I have just purchased some Formalex Green to neutralize our waste 10% formalin for safe drain disposal. I noticed that I can also purchase a "test kit" that will tell me if the aldehydes are safely neutralized. Instead of that, couldn't I just add a drop of Schiff's reagent to the final product, and that would indicate if there are any unreacted aldehydes? Thank you in advance, Stephanie Weaver Texas Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From jcampbell <@t> vdxpathology.com Tue Sep 29 11:59:49 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Tue Sep 29 11:59:54 2009 Subject: [Histonet] GSTP antibody Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82AC149@VDXSERVER01.vdxpathology.local> Hi All, Is anyone out there familiar with GSTP antibody? Do you have any information such as, pretreatment, incubation time, vendors etc? Thanks, Jen From rsrichmond <@t> gmail.com Tue Sep 29 12:32:02 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Sep 29 12:32:08 2009 Subject: [Histonet] Re: formalin neutralization Message-ID: The subject of formalin neutralization is mixed up with trade secrets and bureaucratic nonsense. John Kiernan posted a useful note about ammonia ten years ago: http://www.histosearch.com/histonet/Dec03/RE.HistonetFormaldehydeB.html and there is further useful material in our archives. Sodium bisulfite can also be used and is more convenient to handle, but I don't know what the reaction formula is or how much to use. Does anyone know? Bob Richmond Samurai Pathologist Knoxville TN From Maria.Katleba <@t> stjoe.org Tue Sep 29 14:15:57 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Sep 29 14:16:22 2009 Subject: [Histonet] Voice Recognition In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B67C5@exchangent> References: <4F0B7161A6CD524FAD8017D52E1553400D2B67C5@exchangent> Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDA704AE@SJSNT-SCMAIL03.stjoe.org> Let me know how this goes... I am very interested as well. Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 707-257-4076 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Tuesday, September 29, 2009 6:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Voice Recognition Hello fellow Histonetters - I could use some advice from those who have voice recognition in place at their labs. We are having an online demo given to us soon and I could use some help on any pertinent questions that should be addressed. Are there any concerns that I should be asking about? Would appreciate any pros and cons from all of you. Thank you very much in advance! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From rnewlin <@t> burnham.org Tue Sep 29 14:19:46 2009 From: rnewlin <@t> burnham.org (Robbin Newlin) Date: Tue Sep 29 14:20:04 2009 Subject: [Histonet] test Message-ID: <1EA4D719897F2E41B05237767D806EB63E8E0546B8@MAIL07.burnham.org> Robbin Newlin Manager, Histology Core Facility Burnham Institute for Medical Research 10901 N Torrey Pines Rd. La Jolla, Ca 92037 858-646-3100 ext. 3556 From jengirl1014 <@t> yahoo.com Tue Sep 29 15:33:04 2009 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Tue Sep 29 15:33:08 2009 Subject: [Histonet] Re: Histonet Digest, Vol 70, Issue 38 Message-ID: <148032.18427.qm@web62305.mail.re1.yahoo.com> Has anyone done any capillary staining using VVF?? If so, do you have a protocol that I could use? Thanks so much in advance! Jennifer Sipes Johns Hopkins University ________________________________ From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 29, 2009 1:01:10 PM Subject: Histonet Digest, Vol 70, Issue 38 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. RE: formalin neutralization (Podawiltz, Thomas) ? 2. GSTP antibody (Jennifer Campbell) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Sep 2009 12:27:23 -0400 From: "Podawiltz, Thomas" Subject: RE: [Histonet] formalin neutralization To: 'Stephanie Weaver' , histonet post ??? Message-ID: ??? <38667E7FB77ECD4E91BFAEB8D98638631D38D785D7@LRGHEXVS1.practice.lrgh.org> ??? Content-Type: text/plain; charset="us-ascii" What does your state require? New Hampshire requires to test with the test kit and if at zero we can drain dispose. The state also requires us to keep our test records for five years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Tuesday, September 29, 2009 11:25 AM To: histonet post Subject: [Histonet] formalin neutralization For those who are better at chemistry than I am: I have just purchased some Formalex Green to neutralize our waste 10% formalin for safe drain disposal.? I noticed that I can also purchase a "test kit" that will tell me if the aldehydes are safely neutralized.? Instead of that, couldn't I just add a drop of Schiff's reagent to the final product, and that would indicate if there are any unreacted aldehydes? Thank you in advance, Stephanie Weaver Texas Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL.? This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.? If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 2 Date: Tue, 29 Sep 2009 09:59:49 -0700 From: "Jennifer Campbell" Subject: [Histonet] GSTP antibody To: Message-ID: ??? <5658CBDB9EAE6545ABE50D2563D81BF82AC149@VDXSERVER01.vdxpathology.local> ??? Content-Type: text/plain;??? charset="us-ascii" Hi All, ? Is anyone out there familiar with GSTP antibody?? Do you have any information such as, pretreatment, incubation time, vendors etc? Thanks, Jen ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 70, Issue 38 **************************************** From arvidsonkristen <@t> yahoo.com Tue Sep 29 16:01:06 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Sep 29 16:01:10 2009 Subject: [Histonet] Iodine Message-ID: <54866.20369.qm@web65706.mail.ac4.yahoo.com> I recently found out I can no longer get iodine from my usual vendor.? We use it for verhoff stain (we do our stains by hand).? Any suggestions?? From khicks71 <@t> comcast.net Tue Sep 29 16:08:04 2009 From: khicks71 <@t> comcast.net (khicks71@comcast.net) Date: Tue Sep 29 16:08:07 2009 Subject: [Histonet] microwave processing Message-ID: <1500219451.6810841254258484811.JavaMail.root@sz0061a.emeryville.ca.mail.comcast.net> Can someone let me know the recipe for the solution you make up yourself for processing instead of having to purchasing "Pro-Wave"?? By the way, I absolutely love my microwave processor from Milestone!? I am a Mohs tech and have now opened up a full path lab within our Dermatology practice.? I am very busy but loving every min. of it! Regards, Kathy Hicks H.T.( ASCP ) From NMargaryan <@t> childrensmemorial.org Tue Sep 29 15:56:53 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Sep 29 16:12:55 2009 Subject: [Histonet] acid phosphatase in tissues in routine IHC Message-ID: Hi histonetters, Around a month ago, I asked a question and did not get any answer, but I really would like to have any thoughts from you about the: "Would the presence of acid phosphatase in tissues cause non-specific background staining when doing routine immunoperoxidase staining -- DAB? If answer is "YES": how to block it?" Thanks in advance, Naira From tjasper <@t> copc.net Tue Sep 29 17:02:07 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Sep 29 17:02:14 2009 Subject: [Histonet] MUM 1 antibody References: <61135F0455D33347B5AAE209B903A30429D350FA@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <90354A475B420441B2A0396E5008D4967319BD@copc-sbs.COPC.local> Hi Martha, We run MUM 1. We get it from Cell Marque and run it on our Ventana Benchmark XT. We use a tonsil control and our hemepath likes it just fine. I realize you're running the Bond. Don't know the particulars about protocols for the Bond. We incubate for 32 minutes at 37 degrees (if that helps). You could start there anyway. Good luck, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, September 29, 2009 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MUM 1 antibody I have been asked by my hematopathologist to work up MUM 1 antibody. Does anyone have any suggestions as far as vendor? Any additional advice for the Bond stainer would also be appreciated. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Sep 29 19:50:45 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Sep 29 19:51:17 2009 Subject: [Histonet] Iodine References: <54866.20369.qm@web65706.mail.ac4.yahoo.com> Message-ID: Walgreens? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Tue 9/29/2009 4:01 PM To: histonet Subject: [Histonet] Iodine I recently found out I can no longer get iodine from my usual vendor. We use it for verhoff stain (we do our stains by hand). Any suggestions?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Sep 30 01:38:11 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Sep 30 01:38:19 2009 Subject: [Histonet] BLTS - OT Message-ID: Hey, I thought you meant that famous sandwich ...Bacon Lettuce and Tomato - guess it must be lunchtime On Tue, Sep 29, 2009 at 3:20 PM, Kathleen Jones wrote: > Hello Histonet > > I am wondering if anyone out there is familiar with the Barbeito-Lopez > Trichrome stain. I am using it to identify early myocardial infarction. > My damaged tissue is showing up, but it is very subtle. The papers I've > read show the necrotic myofibers 'popping' out as yellow, whereas mine > are more pale, almost pink. I'm not sure if I am under decolorizing or > if I should leave it in the molybdic acid aniline blue methyl orange > longer. > > Any suggestions would be greatly appreciated! > > Kathy Jones > Research Technician > AVC-UPEI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From SyedJ2 <@t> wyeth.com Wed Sep 30 07:52:06 2009 From: SyedJ2 <@t> wyeth.com (Syed Jameel ) Date: Wed Sep 30 07:52:16 2009 Subject: [Histonet] digest Message-ID: <4AC31C36020000F80001C5FA@gvl011m.gv.us.pri.wyeth.com> From flnails <@t> texaschildrens.org Wed Sep 30 08:14:29 2009 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 30 08:14:49 2009 Subject: [Histonet] test Message-ID: ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Janice.Mahoney <@t> alegent.org Wed Sep 30 08:37:30 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Sep 30 08:37:52 2009 Subject: [Histonet] unsubscribe Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE31296B26@EXCHMBC2.ad.ah.local> ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Janice.Mahoney <@t> alegent.org Wed Sep 30 08:45:22 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Sep 30 08:45:36 2009 Subject: [Histonet] unsubscribe Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE31296B29@EXCHMBC2.ad.ah.local> ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Lesley.Bechtold <@t> jax.org Wed Sep 30 10:11:42 2009 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Wed Sep 30 10:11:50 2009 Subject: [Histonet] staining of plastic sections Message-ID: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried H&E and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) From mcauliff <@t> umdnj.edu Wed Sep 30 10:41:16 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Sep 30 10:39:28 2009 Subject: [Histonet] staining of plastic sections In-Reply-To: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> References: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> Message-ID: <4AC37C1C.8080406@umdnj.edu> Hi Lesley: There is a fairly large literature on this subject. I my opinion, most of the fancier stains are not worth the effort and the results are disappointing. In a separate mailing, I will send you some references. Geoff Lesley Bechtold wrote: > Hi Histonetters, > > If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried H&E and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? > > Thank you! > > Lesley > > > Lesley S. Bechtold > Senior Manager, Histopathology Sciences > The Jackson Laboratory > 600 Main St. > Bar Harbor, ME 04609 > 207-288-6322 (phone) > 207-288-6325 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From katherine-walters <@t> uiowa.edu Wed Sep 30 11:03:18 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Wed Sep 30 11:03:28 2009 Subject: [Histonet] staining of plastic sections In-Reply-To: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> References: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> Message-ID: Perhaps you could try the Paragon stain. It looks more like an H&E. Kathy Walters -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Wednesday, September 30, 2009 10:12 AM To: histonet@lists.utsouthwestern.edu Cc: Pete Finger Subject: [Histonet] staining of plastic sections Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried H&E and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Wed Sep 30 11:49:15 2009 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Wed Sep 30 11:49:20 2009 Subject: [Histonet] pasturella Message-ID: Hello all, We're looking for a place that will do IHC for Pasturella multocida or where we can purchase some antibody to do the test ourselves. Anyone have any ideas? Thanks Denise Long Woodward, MS, HTL (ASCP) QIHC University of Connecticut From kbowden <@t> ucsd.edu Wed Sep 30 11:57:19 2009 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed Sep 30 11:57:39 2009 Subject: [Histonet] staining of plastic sections In-Reply-To: References: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> Message-ID: <4AC38DEF.4070504@ucsd.edu> I use McNeal as a substitute for H&E. I use it on MMA embedded bone. It is to intense (for us) to be used on JB-4. I think you might need to try a few different stains to select the best. */ /* */-- You are what you do - not what you say --/* */Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 3525 John Hopkins Ct. 0863 San Diego, CA 92121-08630 858-822-1251 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* Walters, Katherine S wrote: > Perhaps you could try the Paragon stain. It looks more like an H&E. > > Kathy Walters > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley > Bechtold > Sent: Wednesday, September 30, 2009 10:12 AM > To: histonet@lists.utsouthwestern.edu > Cc: Pete Finger > Subject: [Histonet] staining of plastic sections > > Hi Histonetters, > > If anyone has a good protocol for staining plastic sections, could they > share it? We have samples embedded in an epoxy resin for TEM but they > love the semi-thick sections on the glass slide and asked if we could do > something other than a toluidine blue O, the standard stain for plastic. > We have tried H&E and PAS with no success. If there some way to treat > the sections in order to get them to stain? Is there another stain we > could try? > > Thank you! > > Lesley > > > Lesley S. Bechtold > Senior Manager, Histopathology Sciences > The Jackson Laboratory > 600 Main St. > Bar Harbor, ME 04609 > 207-288-6322 (phone) > 207-288-6325 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > . > > From kdboydhisto <@t> yahoo.com Wed Sep 30 12:24:11 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Sep 30 12:24:14 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date Message-ID: <51925.73063.qm@web58601.mail.re3.yahoo.com> Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. ?I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 ????????(800)-284-0672 Cell?(252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? From Lynn.Burton <@t> Illinois.gov Wed Sep 30 12:31:33 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Sep 30 12:33:23 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date References: <51925.73063.qm@web58601.mail.re3.yahoo.com> Message-ID: Our dilemma has been that we aren't sure how they should be disposed of. We have chemicals that are probably more than 30 years old. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kelly Boyd Sent: Wed 9/30/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suarez <@t> interchange.ubc.ca Wed Sep 30 12:43:59 2009 From: suarez <@t> interchange.ubc.ca (Agripina Suarez) Date: Wed Sep 30 12:40:28 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <19897_1254332051_1254332051_B34ED7468185B44AB6CECF566B1960D0031AE7E5@IL084EX110.illinois.gov> Message-ID: Hi Kelly and Lynn, Can you not ask the surveyor for their reference research literature to indicate that 10 years and older, the chemicals in your list would not be good at all? And if you need to dispose of them, could the surveyor please let you know how to dispose of it? If they want it disposed, they should have a knowledge of how to do it. Just thinking ... Agripina C. Suarez University of British Columbia Shaw Laboratory VGH Research Pavilion, Room 386 828 W. 10th Ave, Vancouver B.C., Canada V5Z 1L8 Phone: 604-875-4111 x68375 Fax: 604-875-4376 email: suarez@interchange.ubc.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Wednesday, September 30, 2009 10:32 AM To: Kelly Boyd; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date Our dilemma has been that we aren't sure how they should be disposed of. We have chemicals that are probably more than 30 years old. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kelly Boyd Sent: Wed 9/30/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.409 / Virus Database: 270.13.115/2405 - Release Date: 09/30/09 10:35:00 From Michael.Owen <@t> fda.hhs.gov Wed Sep 30 12:40:56 2009 From: Michael.Owen <@t> fda.hhs.gov (Owen, Michael P) Date: Wed Sep 30 12:41:01 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <51925.73063.qm@web58601.mail.re3.yahoo.com> Message-ID: <6B1915839B67AD46B88E6BE8F27376BB19FAE4@FMD3VS031.fda.gov> My laboratory had many chemicals that were over 10 years old when the facility started to prepare for ISO 17025 accreditation status four years ago. The facility's current policy for stock chemicals without expiration dates is to set a 5-year expiration period from the date the substance is received by the laboratory. Prepared reagents and working solutions have one-year expiration periods from the dates they are made. Histochemical dyes have been shown worldwide to last for decades in their dried forms. In an emergency, dyes older than five years would probably be acceptable. If a dye can no longer be obtained, the main reason an expired dye is being kept, the method using the dye needs to be updated to use dyes that are currently being manufactured. A good method is one that can reproduced reliably based on readily-available reagents. :o) Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From lpaveli1 <@t> hurleymc.com Wed Sep 30 12:55:25 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Sep 30 12:56:30 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: References: <51925.73063.qm@web58601.mail.re3.yahoo.com> Message-ID: <4AC3634C.59CD.00EE.0@hurleymc.com> When we renovated our lab, we also had many chemicals to discard. We used the same company that hauls our waste xylene away. The company we used was Safety-Kleen, based in Saginaw, Michigan. They came in, made a list of all the chemicals and then gave us a lovely price for disposal/hauling away. Wasn't cheap, but, most importantly, it was disposed of properly, safely, and not down our drains for our fish to grow extra fins! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Burton, Lynn" 9/30/2009 1:31 PM >>> Our dilemma has been that we aren't sure how they should be disposed of. We have chemicals that are probably more than 30 years old. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kelly Boyd Sent: Wed 9/30/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Sep 30 13:27:29 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Sep 30 13:27:36 2009 Subject: [Histonet] staining of plastic sections In-Reply-To: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> References: <3BDA51FFD1A83B4E90829F594A5C37172D1B653C16@JAXBHMAIL01.jax.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23D26@PHSXMB30.partners.org> Under separate cover I sent an article for Stains for Plastic Embedded Tissue Sections which originally appeared in Stain Technology 42:9-14 (1974) by Humphrey and Pittman. I received it as an LKB applicaton note 303. The article describes an "H&E" stain for plastic sections, using methylene blue-azure II and basic fuchsin. I used it a lot, with great success, when I worked at the Mass. Eye and Ear Infirmary. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Wednesday, September 30, 2009 11:12 AM To: histonet@lists.utsouthwestern.edu Cc: Pete Finger Subject: [Histonet] staining of plastic sections Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried H&E and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Maria.Katleba <@t> stjoe.org Wed Sep 30 13:34:43 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Sep 30 13:34:58 2009 Subject: [Histonet] Part Time/Per Diem histotech needed Napa CA Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDA7098A@SJSNT-SCMAIL03.stjoe.org> HISTOTECH Part Time Per Diem Position Available...... Mon-Fri 20-40 hours a week depending on amount of work and needs of the lab ( 330am- 730am 30-80 blocks... Embed and cut.... Path Asst. will stain, coverslip, QC and hand out the slides. Call me if you would like more info. Please, serious applicants only! Requirements: * work the shift as stated above..YES 330am * have 5+ years experience * HT or HTL license (or can sit for the exam) Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center Napa CA 94558 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From mbrereton <@t> lmhosp.org Wed Sep 30 13:46:02 2009 From: mbrereton <@t> lmhosp.org (Brereton, Martha) Date: Wed Sep 30 13:46:09 2009 Subject: [Histonet] PIN-4 Message-ID: In response to the use of Pin 4 staining on various platforms, I have used the PIN 4 from Diagnostic Biosystems on the Ventana Benchmark XT and the results were fantastic! Martha Brereton 860-442-0711 This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From mchavarria <@t> coreplus.com Wed Sep 30 14:26:40 2009 From: mchavarria <@t> coreplus.com (Marbella Chavarria) Date: Wed Sep 30 14:28:37 2009 Subject: [Histonet] PIN-4 In-Reply-To: References: Message-ID: I am using the the PIN 4 from Diagnostics Biosystems on the BOND Max from LEICA I got excellent results too. Regards, Marbella Chavarria -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brereton, Martha Sent: Wednesday, September 30, 2009 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PIN-4 In response to the use of Pin 4 staining on various platforms, I have used the PIN 4 from Diagnostic Biosystems on the Ventana Benchmark XT and the results were fantastic! Martha Brereton 860-442-0711 This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Wed Sep 30 14:55:25 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Sep 30 14:55:29 2009 Subject: [Histonet] Histology Supervisor Position In TX Message-ID: Allied Search Partners, is now accepting resumes for a qualified Histology Supervisor in Corpus Christi, TX area. To apply for this position please send resume to alyssa@alliedsearchpartners.com for intial prescreening of resume. Upon receipt of resume one of our recruiters will hold a confidential phone interview with you. *All inquiries and resume submissions are confidential* POSITION: Histology Supervisor REQUIREMENTS: ASCP Certification At least 3 years experience as a supervisor SHIFT: Monday-Friday TIME: Split Shift, 1-2 days per week histology supervisor will come in from 3am-11am, the remaining days histology supervisor will come in 5am-1pm. OFFER PACKAGE: Benefits package includes medical/dental insurance, PTO, 401K. Relocation expenses ARE offered To apply for this position please send resume to alyssa@alliedsearchpartners.com for intial prescreening of resume. Upon receipt of resume one of our recruiters will hold a confidential phone interview with you. *All inquiries and resume submissions are confidential* *If you know someone interested send a referral and receive a cash bonus* -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From tpodawiltz <@t> lrgh.org Wed Sep 30 15:15:45 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Sep 30 15:18:07 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <51925.73063.qm@web58601.mail.re3.yahoo.com> References: <51925.73063.qm@web58601.mail.re3.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38890A80@LRGHEXVS1.practice.lrgh.org> What CLIA regulation did they use for reference for that statement? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhisto@yahoo.com] Sent: Wednesday, September 30, 2009 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rjbuesa <@t> yahoo.com Wed Sep 30 15:27:26 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 30 15:27:48 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <51925.73063.qm@web58601.mail.re3.yahoo.com> Message-ID: <166856.65682.qm@web65705.mail.ac4.yahoo.com> I was going to comment about "how important" some people?seem to feel to find "something" to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may?"hurt" some feelings! Ren? J. --- On Wed, 9/30/09, Kelly Boyd wrote: From: Kelly Boyd Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. ?I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 ????????(800)-284-0672 Cell?(252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Wed Sep 30 15:40:02 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Sep 30 15:40:01 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <166856.65682.qm@web65705.mail.ac4.yahoo.com> Message-ID: <669D16F3BBF64461B4E52D05EC639635@JimPC> I always have a problem determining the expiration date of sodium chloride! Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about "how important" some people?seem to feel to find "something" to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may?"hurt" some feelings! Ren? J. --- On Wed, 9/30/09, Kelly Boyd wrote: From: Kelly Boyd Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. ?I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 ????????(800)-284-0672 Cell?(252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Sep 30 16:24:10 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Sep 30 16:24:35 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <669D16F3BBF64461B4E52D05EC639635@JimPC> References: <166856.65682.qm@web65705.mail.ac4.yahoo.com> <669D16F3BBF64461B4E52D05EC639635@JimPC> Message-ID: The problem is letting people with little or no knowledge of chemistry make chemical decisions. Some chemicals are unstable on the shelf, some are not. Methyl green decomposes in a year or two, tetracycline decomposes in 5 years, indigo lasts for millennia. Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, September 30, 2009 4:40 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date I always have a problem determining the expiration date of sodium chloride! Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about "how important" some people?seem to feel to find "something" to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may?"hurt" some feelings! Ren? J. --- On Wed, 9/30/09, Kelly Boyd wrote: From: Kelly Boyd Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. ?I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 ????????(800)-284-0672 Cell?(252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Wed Sep 30 16:51:47 2009 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Wed Sep 30 16:52:28 2009 Subject: [Histonet] Non specific Esterase protocol Message-ID: <4AC38CA3020000C500063DD7@nwcl02.tsrh.org> Can somebody share a Non specific esterase Enzyme hsitochemistry protocol that works well with frozen muscle tissue. I have a protocol that I have used for ages but it does not give me a very strong reddish brown staining on my angular fibers. I will be happy to have it and compare. Thank you. Reuel From borisa <@t> trinity-health.org Wed Sep 30 20:20:51 2009 From: borisa <@t> trinity-health.org (Anthony Boris) Date: Wed Sep 30 20:21:03 2009 Subject: [Histonet] Biocare In-Reply-To: <20090929160431.244C654A292C@SB01MSLTW4.trinity-health.org> References: <20090929160431.244C654A292C@SB01MSLTW4.trinity-health.org> Message-ID: <4AC3CBB1.2D52.00DE.0@trinity-health.org> We have some Biocare Intellipath reagents that we are looking to get rid of/donate. No charge but I can not pay to ship them. We are located in metro Detroit. If anyone is interested please send me an email or call. Thanks Tony borisa@trinity-health.org 248-858-6231 From laura_keith <@t> ymail.com Tue Sep 29 18:58:55 2009 From: laura_keith <@t> ymail.com (Ms. Laura Keith) Date: Fri Oct 2 16:46:00 2009 Subject: [Histonet] Used equipment dealers Message-ID: Hello, My name is Laura I recently visited http://lists.utsouthwestern.edu/pipermail/histonet/2009-June/045270.html page and I will like to suggest a useful resource to be added on your site. Title: CoreIndex.com URL: http://www.coreindex.com/ Description : A platform to find worldwide businesses and business partners among importers, exporters, traders and distributors. Thank you very much, Ms. Laura Keith From EDWARDS.DEBORAHK <@t> va.gov Wed Sep 30 15:04:00 2009 From: EDWARDS.DEBORAHK <@t> va.gov (Edwards, Deborah K) Date: Fri Oct 2 16:46:03 2009 Subject: [Histonet] Histobath Frozen Sections Message-ID: <9946FFA3E7ABAF489D048D0F8EA4755404803359@VHAV16MSGA1.V16.Med.VA.Gov> Hello, I am looking for a replacement for my Shandon Histo-bath. It is a snap freeze instrument that I have been using with Isopentane. Do you have something comparable to this or any suggestion? My Histobath finally gave up. Many thanks, Deborah K. Edwards, HTL/QIHC, (ASCP) Supervisor of Histology MEDVAMC Lab 113 2002 Holcombe Houston, Texas 77030 713-794-7259 (v.m.) e-mail: Edwards.deborahk@va.gov 713-794-7260 (histology lab) 713-794-7657 (fax)