[Histonet] IHC Patient Negatives

[Patti Loykasek]

Well, I hope I don¹t start a controversy, but here's my 2 cents worth. I
don't think you should run another negative. If you are going to have any
staining due to intrinsic tissue elements reacting with your detection, you
will see it the first day. I am of the opinion that in most cases too many
negative controls are run. You are wasting precious patient material. With
today's polymer detection systems, there is very little background staining.
If you are doing additional testing on a patient using a different type of
detection, I do think you should run another negative.

Please don't flame me too badly - I'm having a bad day! And it is only my 2
cents worth!


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA






> Hi All,
> 
> 
> 
> I have an issue with my computer, so I cannot see past the little
> paragraphs on the Histonet archives.  Sorry!  This question is probably
> asked many, many times.
> 
> 
> 
> When we run our IHC's we always have a patient negative slide to go with
> our case.  We run everything accept Herceptin on the Bond. When running
> a patient's IHC on the bench and on the Bond we use a negative patient
> control for both.  (We run a test control on all antibodies also)  If we
> are running a double stain IHC also we run another negative patient
> control for that stain also.  The debate we are having is, if the next
> day you run another IHC on the same patient using the same DAB define
> kit, should we be running another negative patient control?
> 
> 
> 
> Thanks for your help!
> 
> 
> 
> Josie Britton HT
> 
> Cheshire Medical Center
> 
> 
> 
> 
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