[Histonet] Green Fluorescent Protein

[Adam .]

At the suggestion from someone on Histonet, I've been using Abcam's chicken
anti-GFP. It's worked great on mouse tissue fixed with paraformaldehyde, Zn
buffered formalin, or 10% neutral buffered formalin. Even though Abcam
suggests that you antigen retrieve, I've found it works just as well without
it.

As for viewing GFP without any antibody, you apparently can, at least in
bone. See
http://skeletalbiology.uchc.edu/30_ResearchProgram/304_gap/index.htm for a
really detailed discussion. I've tried before and it hasn't worked... I
think you need really high quality optics and filter cubes.

Adam

On Tue, Oct 27, 2009 at 8:50 AM, Paula Pierce <
contact <@t> excaliburpathology.com> wrote:

> Hello,
>
> I routinely use Invitrogen's Rabbit anti-GFP on mouse and rat FFPE tissue.
>
> Paula
>
>
>
>
> ________________________________
> From: Melanie Black <Melanie.Black <@t> uct.ac.za>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Tue, October 27, 2009 7:48:48 AM
> Subject: [Histonet] Green Fluorescent Protein
>
> Hi
>
> I am looking to demonstrate Green fluorescent protein in Para formaldehyde
> fixed, processed rat tissue. Apart from using an antibody against GFP, can
> the native GFP be detected? Gayle Callis, I believe you may be able to help
> me with this method.
>
> Thanks
> Melanie
>
> Melanie Black
> 082 469 3352
>
> Cardiovascular Research Unit
> 3rd Floor; Chris Barnard Building
> Medical School;
> Observatory. 7925.
> University of Cape Town.
> South Africa.
>
>
>
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