[Histonet] Floaters in Waterbath

Mon Oct 26 09:52:58 CDT 2009

Agree that phone book paper works great on the water. Picked up that tip many years ago from the AFIP.

Brett

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Monday, October 26, 2009 8:05 AM
To: Angela Bitting; Jackie M O'Connor; Stella Mireles; histonet-bounces <@t> lists.utsouthwestern.edu
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Floaters in Waterbath

I have always used pieces of phone book paper.  Just ask everyone to bring in their old phone books, and tear the papers off at the seam.  They provide just the right amount of absorption, are the perfect size, are free and a useful way to recycle!

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

________________________________
From: Angela Bitting <akbitting <@t> geisinger.edu>
To: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>; Stella Mireles <estellamireles <@t> gmail.com>; histonet-bounces <@t> lists.utsouthwestern.edu
Cc: Histonet <@t> lists.utsouthwestern.edu
Sent: Fri, October 23, 2009 10:40:58 AM
Subject: Re: [Histonet] Floaters in Waterbath

We currently have a Quality Improvement Plan in effect to address this issue.
Jackie is right about keeping those forcep wells clean. 
Although we don't swipe Kimwipes over our waterbath after each block, we do it very regularly.
Another thing to consider is how often you clean your embedding molds.

~Angie

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

!

>>> "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com> 10/23/2009 10:23 AM >>>
Kim wipes seem to pick up more debris than paper towels, and they pick up 
much less water.  We routinely sweep the waterbath with a kimwipe after 
each block.  You can also pick up floaters from embedding if the forceps 
are not cleaned between each block.  Most embedding centers have multiple 
wells for forceps - how often do you clean those wells?  You'd be amazed 
at how much gunk accumulates in there! 

From:
Stella Mireles <estellamireles <@t> gmail.com>
To:
Histonet <@t> lists.utsouthwestern.edu 
Date:
10/23/2009 09:11 AM
Subject:
[Histonet] Floaters in Waterbath
Sent by:
histonet-bounces <@t> lists.utsouthwestern.edu 

I know we have all had some problems with floaters in our waterbath at 
some
point in our microtomy career.
Our doctors are very picky and I need some tips on keeping an immaculate
clean waterbath, but not sacrificing the speed in a regular
routine lab.  We use the pyrex waterbath and paper towels for wiping our
area.

Thanks
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