[Histonet] Clear Paraffin? (Monfils, Paul)
Dessasau III, Evan
eddessa <@t> emory.edu
Wed Oct 14 12:31:24 CDT 2009
....IMEB, INC. had a clear paraffin at the NSH meeting in birmingham. They are at www.IMEBINC.com
E-van
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, October 14, 2009 1:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 71, Issue 14
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Today's Topics:
1. image pro plus (Sarka Lhotak)
2. price for plastic embedding (Nancy W. Troiano)
3. Clear Paraffin? (Monfils, Paul)
4. Transporting Formalin (Feher, Stephen)
5. New Lead Tech/Assistant Manager position available in NJ
(Brian- Prometheus)
6. how to fix EGFP for proteomics (Lilja Thoenes)
7. Pathos Tissue Processor vs Tissue Xpress (Kiranjit Grewal)
8. hematoxylin and nova red staining (del phillips)
9. looking for calibration tools for Optimax plus stainer
(Leroy Brown)
10. Re: looking for calibration tools for Optimax plus stainer
(Patti Loykasek)
11. Isotype background (Adam .)
12. RE: Automated H&E stainers (Cathy)
13. RE: Transporting Formalin (DELIA GARCIA)
14. lid gasket for processor (Diana McCaig)
15. pkh (Melissa Mazan)
16. RE: lid gasket for processor (Rathborne, Toni)
17. Fw: Pathos Tissue Processor vs Tissue Xpress (Rene J Buesa)
18. Re: Isotype background (Merced M Leiker)
19. RE: Fw: Pathos Tissue Processor vs Tissue Xpress (Rene J Buesa)
20. HerCep (kkwaa <@t> bidmc.harvard.edu)
21. unsubscribe, please (susan bryant)
22. RELIA Histology Job Alert Histotechnologist needed in Atlanta
(Pam Barker)
23. Re: unsubscribe, please (Emily Sours)
24. Re: unsubscribe, please (Peter Carroll)
----------------------------------------------------------------------
Message: 1
Date: Tue, 13 Oct 2009 13:30:14 -0400
From: "Sarka Lhotak" <lhotaks <@t> mcmaster.ca>
Subject: [Histonet] image pro plus
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <web-273091418 <@t> cgpsrv2.cis.mcmaster.ca>
Content-Type: text/plain;charset=utf-8
Hi Hatem,
to separate RGB colours, go to Process/ Colour channel/ Extract. This
will give you each channel in B&W, i.e. each pixel with intensity from
0 to 255 for Red, Blue and Green component of your image.
There must be many ways how to do intensity. One that would be possible
is: go to Process/ Pseudocolour/ Select number of divisions (bins).
Intensities will then be divided into bins, say 0 to 10, 11 to 20, 21
to 30 etc. all the way to 255. And then look in areas, it will give you
number of pixels for each range of intensities you selected in
Divisions.
Hope it helps,
Sarka Lhotak, PhD
McMaster University, Hamilton
------------------------------
Message: 2
Date: Tue, 13 Oct 2009 15:26:34 -0400
From: "Nancy W. Troiano" <nancy.troiano <@t> yale.edu>
Subject: [Histonet] price for plastic embedding
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.2.1.1.2.20091013152448.0145d820 <@t> email.med.yale.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Our lab prices for plastic embedding are dependent on many variables, e.g.
size of bone tissue, implant/nonimplant, services required (such as embed
only, embed, cut, etc.). You can contact me for pricing for work done for
outside institutions.
------------------------------
Message: 3
Date: Tue, 13 Oct 2009 15:58:46 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: [Histonet] Clear Paraffin?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4EBFF65383B74D49995298C4976D1D5E03835CEF <@t> LSRIEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
I work in a core histology facility where I receive all kinds of specimens and embedded blocks from many different sources. Occasionally I receive blocks that are embedded in a clear (transparent, not white) embedding wax. Does anyone know what this product is?
------------------------------
Message: 4
Date: Tue, 13 Oct 2009 16:23:10 -0400
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: [Histonet] Transporting Formalin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<73A7ED895EE0C24D9267ED814911DF190FDB207F <@t> exchange.cmc-nh.org>
Content-Type: text/plain; charset="us-ascii"
Hi gang,
A question came up in a committee meeting today surrounding how other
institutions are transporting quantities of 10% NBF from a main facility
to outlying hospitals and outpatient clinics. The quantities in
question ranged from 5 - 20 gallons of formalin in sealed carboy type
containers. Does a main hospital or lab distribute the formalin to
outlying facilities or are outlying facilities having to order direct
from a supplier and bill the main facility?
Some places just order it and transport via courier. This may be
restricted due to various State EPA rules. How are you all doing this?
Thanks,
Steve
Stephen A. Feher, MS, SCT (ASCP)
Pathology Supervisor
Catholic Medical Center
100 McGregor Street
Manchester, NH 03102
603-663-6707
sfeher <@t> cmc-nh.org <mailto:sfeher <@t> cmc-nh.org>
------------------------------
Message: 5
Date: Tue, 13 Oct 2009 16:52:32 -0400
From: "Brian- Prometheus" <brian <@t> prometheushealthcare.com>
Subject: [Histonet] New Lead Tech/Assistant Manager position available
in NJ
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001901ca4c47$15e77600$41b66200$@com>
Content-Type: text/plain; charset="us-ascii"
New Opening with a lab in Paramus
Day shift 5am to 1:30pm or 6:00a to 2:30pm
Stresses quality over quantity
Prefers ASCP certification and NY state licensure
Someone who can multitask, detail oriented
IHC experience a plus
Salary will commensurate upon experience
Minimum 10 yrs exp
Lab is growing, consistently acquiring new clients. Been in business about a
year
Please let me know if you may be interested!
Brian Feldman
Principal
Prometheus Healthcare
Office 301-693-9057
Fax 301-368-2478
brian <@t> prometheushealthcare.com <mailto:brian <@t> prometheushealthcare.com>
www.prometheushealthcare.com <http://www.prometheushealthcare.com/>
*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***
http://twitter.com/PrometheusBlog
------------------------------
Message: 6
Date: Fri, 9 Oct 2009 16:01:23 +0100
From: Lilja Thoenes <liljathoenes <@t> rcsi.ie>
Subject: [Histonet] how to fix EGFP for proteomics
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<54E3312540DE1547A410FA8A333AC8F00223FA4089 <@t> RCSIEXCHANGE.rcsi-internal.ie>
Content-Type: text/plain; charset="us-ascii"
Hi ,
I plan isolate GFPtagged cells from cryosections via laser microdissection for proteomics but still I am struggling with not loosing the GFP signal. Does anyone has a good idea how to fix the signal so that afterwards proteomics is still possible?
Thanks a lot,
Lilja
Lilja Thoenes
Dept. of Neurodegeneration and Physiology
Royal College of Surgeons in Ireland (RCSI)
123 St. Stephens Green, Dublin 2, Ireland
Office: +353 1 402 2794
Fax: +353 1 402 2447
------------------------------
Message: 7
Date: Sun, 11 Oct 2009 21:07:39 -0700
From: "Kiranjit Grewal" <kiran_g <@t> sbcglobal.net>
Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <277641.14522.qm <@t> smtp101.sbc.mail.sp1.yahoo.com>
Content-Type: text/plain; charset="us-ascii"
Hi,
Anybody has anything to share regarding Pathos both good and bad. We are
planning to get one.
Also please share if you have any experience with Tissue Xpress.
Thanks,
Histotech in CA
------------------------------
Message: 8
Date: Mon, 12 Oct 2009 10:36:08 -0500
From: "del phillips" <dphillips <@t> vetmed.lsu.edu>
Subject: [Histonet] hematoxylin and nova red staining
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: dphillips33 <@t> juno.com
Message-ID: <000001ca4b52$2f60e080$8e22a180$@lsu.edu>
Content-Type: text/plain; charset="us-ascii"
If hematoxylin is place on a slide before the nova red is it too late to add
the nova red after the hematoxylin?
Thanks
------------------------------
Message: 9
Date: Tue, 13 Oct 2009 14:10:39 -0700
From: Leroy Brown <rhbrown1 <@t> histocs.com>
Subject: [Histonet] looking for calibration tools for Optimax plus
stainer
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1255539601.17299.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="iso-8859-1";
Does anyone have a set of calibration tools needed to calibrate my BioGenex Optimax stainer. Seems they no longer make these or sell time??
thanks
LeRoy Brown Ht(ASCP) HTL
www.histocs.com
360-966-7300
------------------------------
Message: 10
Date: Tue, 13 Oct 2009 14:46:37 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: Re: [Histonet] looking for calibration tools for Optimax plus
stainer
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C6FA434D.1B22F%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
In reference to LeRoy's last statement below- if anyone is selling time
please put me on the list for the maximum allowable! I haven't been able to
figure out how to cram more hours in a day!
And it's only Tuesday.
Patti Loykasek
> Does anyone have a set of calibration tools needed to calibrate my BioGenex
> Optimax stainer. Seems they no longer make these or sell time??
>
> thanks
>
> LeRoy Brown Ht(ASCP) HTL
>
> www.histocs.com
>
> 360-966-7300
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
This e-mail message, including any attachments, is for the sole use of the
intended recipients and may contain privileged information. Any unauthorized
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recipient, please contact the sender by e-mail and destroy all copies of the
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------------------------------
Message: 11
Date: Tue, 13 Oct 2009 16:48:46 -0500
From: "Adam ." <anonwums1 <@t> gmail.com>
Subject: [Histonet] Isotype background
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<858249120910131448v26622f8dtd5599dbbf64e67e4 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi all,
I am trying some IHC, and I am having a peculiar problem. Like I expect, my
antibody of interest (anti-mouse goat polyclonal) stains nonspecifically at
high concentrations (10 ug / ml) but as I titer it down (3 ug / mL), it
seems to stain relatively specifically the cells I think it should stain.
However, at 3 ug / mL, my isotype goat IgG stains nearly everything.
Here is my protocol
1) Block in 3% H2O2 for 10'. Wash.
2) Block in 10% donkey serum for 1 hr. Wash.
3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits)
4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. Wash.
5) Incubate with secondary (biotinylated donkey anti-goat, cross adsorbed to
mouse) in 2% donkey serum for 1 hr at room temp. Wash.
6) Incubate with strepavidin HRP in TBS-T for 30'. Wash.
7) Incubate with DAB+ (Dako) for 5'.
For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson.
Some people have suggested that I just do away with isotypes altogether and
use a no primary control instead. I think there is some merit to this idea,
but I still think my issue might be indicative of a larger technical problem
in my staining protocol.
Thanks,
Adam
------------------------------
Message: 12
Date: Tue, 13 Oct 2009 19:10:08 -0700
From: "Cathy" <cfitz <@t> telus.net>
Subject: RE: [Histonet] Automated H&E stainers
To: "'Bauer, Karen L.'" <Bauer.Karen <@t> mayo.edu>, "'Connolly, Brett M'"
<brett_connolly <@t> merck.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9EFA5FE9872A49718A169C823AD49321 <@t> your8ba846406f>
Content-Type: text/plain; charset="us-ascii"
Have you had any problems with the Coverslipper? We purchased the Prisma
with linked Coverslipper last March and have had on going problems with the
Coverslipper jamming with error code -Motor ejector error E15. We have
contacted Somagen and were told that this is normal. It is happening at
least once a day and sometimes more. We love the stainer and if the
Coverslipper wasn't jamming we would love it just as much.
What do you do with the coverslipped slides? We find that they don't dry as
quickly as they did on the older film Coverslipper. We don't have a vented
sign out area so they are staying on the carousel until they are mostly dry.
Cathy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bauer, Karen
L.
Sent: Monday, October 12, 2009 5:42 AM
To: Connolly, Brett M; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Automated H&E stainers
Brett,
We purchased the Tissue Tek PRISMA with the attached film coverslipper a
year ago and LOVE IT!! Was a little nervous switching from the glass
coverslips to the film, but the docs love the slides and we have had no
problems. Very easy to use and we like the fact that we can file the
slides the same day without having to wait for them to "dry".
Karen
Karen L. Bauer HTL (ASCP)
Histology Section Chief
Department of Pathology - Luther Hospital
Luther Midelfort - Mayo Health System
715-838-3205
bauer.karen <@t> mayo.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Friday, October 09, 2009 8:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Automated H&E stainers
Which one do you like?...pro&cons?
Thanks,
Brett
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com
Notice: This e-mail message, together with any attachments, contains
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------------------------------
Message: 13
Date: Tue, 13 Oct 2009 21:52:57 CDT
From: "DELIA GARCIA" <deliadfam <@t> yahoo.com>
Subject: RE: [Histonet] Transporting Formalin
To: "Feher, Stephen"
<sfeher <@t> CMC-NH.ORG>,histonet <@t> lists.utsouthwestern.edu
Message-ID:
<mailman.1.1255539601.17299.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="iso-8859-1"
We have various shipping addresses with our supplier. Which allows us to order directly for our clients. We incur all costs including the associated Hazardous Materials handling fee. We also use courier for in-town clients. Hope this helps.
Delia Garcia
Lead Histotechnologist
Tucson, AZ
-----Original Message-----
Date: Tuesday, October 13, 2009 1:36:33 pm
To: <histonet <@t> lists.utsouthwestern.edu>
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: [Histonet] Transporting Formalin
Hi gang,
A question came up in a committee meeting today surrounding how other
institutions are transporting quantities of 10% NBF from a main facility
to outlying hospitals and outpatient clinics. The quantities in
question ranged from 5 - 20 gallons of formalin in sealed carboy type
containers. Does a main hospital or lab distribute the formalin to
outlying facilities or are outlying facilities having to order direct
from a supplier and bill the main facility?
Some places just order it and transport via courier. This may be
restricted due to various State EPA rules. How are you all doing this?
Thanks,
Steve
Stephen A. Feher, MS, SCT (ASCP)
Pathology Supervisor
Catholic Medical Center
100 McGregor Street
Manchester, NH 03102
603-663-6707
sfeher <@t> cmc-nh.org <mailto:sfeher <@t> cmc-nh.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 14 Oct 2009 06:33:32 -0400
From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] lid gasket for processor
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCFD9E6A390E294AAF3A2561CD32E5C40D577914 <@t> ckhamail1.ckha.on.ca>
Content-Type: text/plain; charset="us-ascii"
Our lid gasket seemed to have grown overnight and expanded considerable
in length. It is difficult to re-seat it back in. It appears too loose
and when the processor cycles through a station and we open the lid, it
is laying on the edge of the retort. Does anyone have any ideas what we
can do until a new gasket can be ordered?
Diana
------------------------------
Message: 15
Date: Wed, 14 Oct 2009 08:22:21 -0400
From: Melissa Mazan <melissa.mazan <@t> tufts.edu>
Subject: [Histonet] pkh
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4AD5C27D.5010601 <@t> tufts.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi all,
We have collected a specific lung cell via FACS from mice, stained the
cells with PKH, and then returned the cells to congenic mice via 1.
tracheal injection or 2. tail vein injection. We can see the PKH
positive cells in the recipient lungs on microscopy, but we are having a
real problem costaining these cells with anything else such as vimentin
- has anyone else tried this approach? Melissa
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel: 508-839-5395
fax: 508-839-7903
email: melissa.mazan <@t> tufts.edu
------------------------------
Message: 16
Date: Wed, 14 Oct 2009 09:26:52 -0400
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] lid gasket for processor
To: "Diana McCaig" <dmccaig <@t> ckha.on.ca>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E78340C766A5284D999F5F5891DDF8900BAF8EC5 <@t> smcmail.somerset-healthcare.com>
Content-Type: text/plain; charset="utf-8"
You might be able to shrink it a little by putting the gasket in the refrigerator for a while.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Diana
McCaig
Sent: Wednesday, October 14, 2009 6:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lid gasket for processor
Our lid gasket seemed to have grown overnight and expanded considerable
in length. It is difficult to re-seat it back in. It appears too loose
and when the processor cycles through a station and we open the lid, it
is laying on the edge of the retort. Does anyone have any ideas what we
can do until a new gasket can be ordered?
Diana
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Message: 17
Date: Wed, 14 Oct 2009 06:30:14 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <605622.63517.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
--- On Wed, 10/14/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Pathos Tissue Processor vs Tissue Xpress
To: "Kiranjit Grewal" <kiran_g <@t> sbcglobal.net>
Date: Wednesday, October 14, 2009, 9:28 AM
Under separate cover I am sending you an article where both instruments (as well as some others) are compared in?reurn/investment and effectiveness.
Ren? J.
--- On Mon, 10/12/09, Kiranjit Grewal <kiran_g <@t> sbcglobal.net> wrote:
From: Kiranjit Grewal <kiran_g <@t> sbcglobal.net>
Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, October 12, 2009, 12:07 AM
Hi,
Anybody has anything to share regarding Pathos both good and bad. We are
planning to get one.
Also please share if you have any experience with Tissue Xpress.
Thanks,
Histotech in CA
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Wed, 14 Oct 2009 10:20:41 -0400
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Isotype background
To: "Adam ." <anonwums1 <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID: <31878536799288738D402C8A <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
I wouldn't get rid of the isotype control altogether; it's fulfilling it's
purpose in trying to tell you something. Have you tried a no-primary
control in parallel with your isotype control to see what the secondary is
binding to - the tissue or bound goat IgG? What type of tissue is it? Maybe
the goat IgG is binding to Fc receptors in the tissue. Try an Fc block. Or
try a different goat IgG or one from a different company.
But it may not even be a big issue; your goat IgG is not giving you the
specific stain pattern that you are observing with your primary, right?
It's just giving a lot of background? Instead of spending a lot of time
troubleshooting the high background issue with your isotype control, it's
already succeeded in telling you that your primary is specific, which is
what you ultimately wanted to know, so you could just take that for what
it's worth and go from there...
Just my two cents' worth!
Regards,
Merced
--On Tuesday, October 13, 2009 4:48 PM -0500 "Adam ." <anonwums1 <@t> gmail.com>
wrote:
> Hi all,
>
> I am trying some IHC, and I am having a peculiar problem. Like I expect,
> my antibody of interest (anti-mouse goat polyclonal) stains
> nonspecifically at high concentrations (10 ug / ml) but as I titer it
> down (3 ug / mL), it seems to stain relatively specifically the cells I
> think it should stain. However, at 3 ug / mL, my isotype goat IgG stains
> nearly everything.
>
> Here is my protocol
> 1) Block in 3% H2O2 for 10'. Wash.
> 2) Block in 10% donkey serum for 1 hr. Wash.
> 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits)
> 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum.
> Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross
> adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash.
> 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash.
> 7) Incubate with DAB+ (Dako) for 5'.
>
> For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson.
> Some people have suggested that I just do away with isotypes altogether
> and use a no primary control instead. I think there is some merit to this
> idea, but I still think my issue might be indicative of a larger
> technical problem in my staining protocol.
>
> Thanks,
> Adam
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 19
Date: Wed, 14 Oct 2009 07:22:38 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: RE: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress
To: Donna Willis <donna <@t> milestonemed.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <158309.9167.qm <@t> web65701.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Donna:
Thank you for your comment. As you write if the Pathos Classic is still available what I wrote is still valid. Unfortunately when somebody writes an article the conclusions remain valid as long as the subjects don't change, but the approach remains.
As you can see I have forwarded your comments and my answer for all to read in HistoNet so they can benefit of it.
As to going to Kalamazzo I really appreciate your invitation but I seldom travel ("retired with a fixed income", you the drill!).
Regards
Ren? J.
--- On Wed, 10/14/09, Donna Willis <donna <@t> milestonemed.com> wrote:
From: Donna Willis <donna <@t> milestonemed.com>
Subject: RE: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Date: Wednesday, October 14, 2009, 9:58 AM
Rene,
There are now 2 Pathos units available to customers.? The Pathos Classic and the Pathos Delta.? You article was written before the Delta was on the market.? Please understand that giving this article to customers may not give them a true picture of the unit they are looking to purchase.? If you are interested in coming to the Milestone Kalamazoo office to experience the whole Milestone instrument line, we would love to have you.
Sincerely,
Donna Willis, HT/HTL(ASCP)
North American Application Manager
Milestone Medical
(866) 995-5300 toll free
(269) 488-4040 fax
www.milestonemed.com
Helping Patients
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 14, 2009 8:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress
--- On Wed, 10/14/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Pathos Tissue Processor vs Tissue Xpress
To: "Kiranjit Grewal" <kiran_g <@t> sbcglobal.net>
Date: Wednesday, October 14, 2009, 9:28 AM
Under separate cover I am sending you an article where both instruments (as well as some others) are compared in?reurn/investment and effectiveness.
Ren? J.
--- On Mon, 10/12/09, Kiranjit Grewal <kiran_g <@t> sbcglobal.net> wrote:
From: Kiranjit Grewal <kiran_g <@t> sbcglobal.net>
Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, October 12, 2009, 12:07 AM
Hi,
Anybody has anything to share regarding Pathos both good and bad. We are
planning to get one.
Also please share if you have any experience with Tissue Xpress.
Thanks,
Histotech in CA
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
? ? ?
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------------------------------
Message: 20
Date: Wed, 14 Oct 2009 10:25:51 -0400
From: kkwaa <@t> bidmc.harvard.edu
Subject: [Histonet] HerCep
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1453B63A4BE95441AA65F577CEE7B6E104E16997F0 <@t> EVS5CCR.its.caregroup.org>
Content-Type: text/plain; charset=us-ascii
Does anyone have any experience using the Dako HerCep kit?
And do you have any problems with cytoplasmic staining?
------------------------------
Message: 21
Date: Wed, 14 Oct 2009 11:24:44 -0400
From: susan bryant <sbryant <@t> labpath.com>
Subject: [Histonet] unsubscribe, please
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4AD5ED3C.20805 <@t> labpath.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I wish to unsubscribe and return in 6 weeks.
Thank you,
Susan E. Bryant
Knoxville Dermatopathology
------------------------------
Message: 22
Date: Wed, 14 Oct 2009 11:42:07 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Job Alert Histotechnologist needed
in Atlanta
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1My5zM-0005Zb-He <@t> elasmtp-curtail.atl.sa.earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!
I have a new position that I want to let everyone know about. I am
assisting a client located in Atlanta GA that is in need of a
histotechnologist.
This is a full time permanent position on the night shift
(12:30a-8:30a). My client offers excellent benefits and compensation
and a generous shift differential.
ASCP certification and strong experience in grossing is required.
For more information please contact Pam Barker at 866-607-3542 or
relia1 <@t> earthlink.net
Thank You!
Pam Barker
President
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
Toll Free: (866)607-3542
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net <mailto:relia1 <@t> earthlink.net>
<<http://home.earthlink.net/~relia1>>
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia
------------------------------
Message: 23
Date: Wed, 14 Oct 2009 12:34:59 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] unsubscribe, please
To: susan bryant <sbryant <@t> labpath.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b39794b0910140934v3417513fr82ad43a9ecad2f2a <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Good for you!
You should probably FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY
TIME SOMEONE WRITES IN TO UNSUBSCRIBE.
Emily
...the thrill of being close to that hidden knowledge. That's the way I feel
when I read Nabokov. Encrypted within his words, encoded indecipherably,
ambiguously, is the equivalent of the secret of lightning. Something akin to
the secret code of higher human consciousness, the DNA, the genome of
genius.
-Ron Rosenbaum
On Wed, Oct 14, 2009 at 11:24 AM, susan bryant <sbryant <@t> labpath.com> wrote:
> I wish to unsubscribe and return in 6 weeks.
>
> Thank you,
> Susan E. Bryant
> Knoxville Dermatopathology
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 24
Date: Wed, 14 Oct 2009 12:56:08 -0400
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] unsubscribe, please
To: Emily Sours <talulahgosh <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu, susan bryant
<sbryant <@t> labpath.com>
Message-ID: <4AD602A8.6050306 <@t> umdnj.edu>
Content-Type: text/plain; charset=UTF-8; format=flowed
>FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE
WRITES IN TO UNSUBSCRIBE
Histonet Fun-Fact:
Hits for the word "unsubscribe" in the archive: 1144
Hits for the word "eosin" in the archive: 801
Perhaps we should rename this list "Unsubscribenet" ;)
------------------------------
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End of Histonet Digest, Vol 71, Issue 14
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