[Histonet] need help in selecting blades for Microtome Leitz1512and good place for service

[Armandi, Arlene]

Hi Bader,
I've been a Histotechnologist for 30 years, and the Leitz 1512 is the
best microtome I have ever used. (And I've used a lot)I can easily cut
1-2 micron sections of any tissue, including bone. 
I don't know where you are located, but if you are anywhere near Los
Angeles, "Fritz Instruments" serviced my three 1512s for years and
always did an excellent job. They are great with older microtomes.
As for disposable blades, the Tissue-Tek Accu-Edge blades are the best
on the market.
 If you are looking for the knife holder that is part of the original
instrument, try IMEB instruments, they carry used 1512s and probably
have some spare parts. Ph# 800-543-8496. or imebinc.com
If you are looking for the blade holder that fits into the 1512's knife
holder, that is Tissue-Tek #4687. IMEB also carries that, as does EMS (
Electron Microscopy Sciences)  Cat# 63048-10  800-523-58874 
A couple of hints/suggestions for using the 1512...
-When you trim your blocks, don't "rock" the handwheel, make complete
rotations. 
-Oil the unit weekly (if you look under the top lid you should see that
the oil ports are marked.)
- Lubricate the Teflon plate (black plate under the knife holder) at
least weekly (more often if heavy use). Use microtome grease (not oil)
for this. 
- I have always had to remove the knife guards on the unit to allow for
using a disposable blade holder. The units were originally made to be
used with the old reusable "hard knives", thus they were made with the
knife guards built in.
 Hope some of this helps. If you have any other questions about the
1512, please feel free to call or e-mail me. 



Arlene Armandi, HTL, HT(ASCP)
Cedars-Sinai Medical Center
Los Angeles, CA 90048
310-423-4756
arlene.armandi <@t> cshs.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bader
Siddiki
Sent: Monday, October 19, 2009 3:37 PM
To: Histonet
Subject: [Histonet] need help in selecting blades for Microtome
Leitz1512and good place for service

Hello Histonets
I need some help and advice from you experts.
We are new in cutting formalin fixed paraffin embedded (FFPE) tissue
sections.
We need your advice.
We have a used Leitz 1512 microtome.
------- perhaps it needs service, can you recommend a good and
reasonable
place.
--------what  will be a good disposable blades to buy for cutting FFPE
tissues sections (mostly soft tissues like colon, stomach, skin, cancer
tissues  etc).
--------this  instrument needs a blade holder, what would you recommend,
Leitz does not sell blade holders for this instrument anymore.
--------any good technique for cutting tissues for a person who is not
expert.
--------good place to buy formalin fixed tissue blocks for research use.

Thanks a million.
Bader

e-mail address: baderbo <@t> gmail.com
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