[Histonet] black DAB instead of brown

[Paula Pierce]

The cause for DAB to turn black would be the introduction of a metal such as nickel or iron.




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From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, October 8, 2009 12:15:50 PM
Subject: [Histonet] black DAB instead of brown

Dear Histometters:

I am getting black staining with my DAB instead of brown.
I use usual protocol with AR-pH6, H2O2, Avidin/Biotin, PB, 1º, biotinylated 2-dary, streptavidin then DAB.

Does any of use know this kind of problem? and What exactly can coast this artifact?

Thanks in advance,
Naira

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