[Histonet] CD3 on mouse tissue

Jennifer Campbell jcampbell <@t> vdxpathology.com
Wed Oct 7 09:40:48 CDT 2009


Hi Histonetters,
 
  I'm trying to work up CD3 on mouse tissue.  I knew I would have to get
a mouse-on-mouse kit since CD3 is a rabbit polyclonal but do you use
some sort of rodent kit to eliminate background staining?  My secondary
antibody is part of a multilink kit from Biogenex and is capable of
recognizing both mouse and rabbit primary antibodies.  So, I'm afraid
that it is recognizing endogenous antibodies in my mouse tissue.  Any
recommendations?  
 
Thanks,
 
Jennifer


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