[Histonet] CD3 on Mouse Tissue

[John Shelley]

Hello Fellow Histonetters,

I have been trying to optimize CD3 from Abcam (ab56313) for quite awhile now and I am at my wits end with it. I have tried various retrieval methods and dilutions and polymer kits all to no avail. Has any researcher out there worked with this CD3 before or has a CD3 marker that they found to work and would you be willing to share what you did to get it to work.  I have this group breathing down my neck because they wanted their results by last week.  Any help would be greatly appreciated, thanks!!!

John