From kdboydhisto <@t> yahoo.com Thu Oct 1 07:09:15 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Oct 1 07:09:19 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date Message-ID: <538683.628.qm@web58604.mail.re3.yahoo.com> They did not reference any CLIA regulation. It was just suggested that the dry chemicals were not very useful after 10 years. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 ????????(800)-284-0672 Cell?(252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? --- On Wed, 9/30/09, Podawiltz, Thomas wrote: From: Podawiltz, Thomas Subject: RE: [Histonet] discarding old dry chemicals with no expiration date To: "Kelly Boyd" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, September 30, 2009, 4:15 PM What CLIA regulation did they use for reference for that statement? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhisto@yahoo.com] Sent: Wednesday, September 30, 2009 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 ? ? ? ? (800)-284-0672 Cell (252)-943-9527 Fax? (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL.? This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.? If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From jshelley <@t> burnham.org Thu Oct 1 07:48:03 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Thu Oct 1 07:48:08 2009 Subject: [Histonet] CD3 on Mouse Tissue Message-ID: Hello Fellow Histonetters, I have been trying to optimize CD3 from Abcam (ab56313) for quite awhile now and I am at my wits end with it. I have tried various retrieval methods and dilutions and polymer kits all to no avail. Has any researcher out there worked with this CD3 before or has a CD3 marker that they found to work and would you be willing to share what you did to get it to work. I have this group breathing down my neck because they wanted their results by last week. Any help would be greatly appreciated, thanks!!! John From billodonnell <@t> catholichealth.net Thu Oct 1 09:05:08 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Oct 1 09:05:23 2009 Subject: [Histonet] discarding old dry chemicals with no expiration date In-Reply-To: <669D16F3BBF64461B4E52D05EC639635@JimPC> References: <166856.65682.qm@web65705.mail.ac4.yahoo.com> <669D16F3BBF64461B4E52D05EC639635@JimPC> Message-ID: Here's the reference I use for that problem. But if the salt loses its saltiness, how can it be made salty again? It is no longer good for anything, except to be thrown out and trampled by men. Mk 9:50(ish or so. :) Happy Friday -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, September 30, 2009 3:40 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date I always have a problem determining the expiration date of sodium chloride! Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about "how important" some people?seem to feel to find "something" to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may?"hurt" some feelings! Ren? J. --- On Wed, 9/30/09, Kelly Boyd wrote: From: Kelly Boyd Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. ?I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 ????????(800)-284-0672 Cell?(252)-943-9527 Fax? (252)-830-0032 ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Cordero <@t> comphealth.com Thu Oct 1 09:15:07 2009 From: Robert.Cordero <@t> comphealth.com (Robert.Cordero@comphealth.com) Date: Thu Oct 1 09:15:50 2009 Subject: [Histonet] Histology Supervisor Needed in Georgia In-Reply-To: Message-ID: Hello all! Just wanted to see if anyone out there was interested or knew of someone looking for a Supervisory opportunity in Georgia? The facility is offering relocation assistance for non local applicants! Clinical Histology Supervisor - Full-time - 8a-5p Job Summary: Collects and prepares specimens for histological testing procedures and assumes rapid and accurate results. Prepares microscopic slides from surgery and autopsy specimens for examinations. Provides direct supervision of Histotechnicians, Tech Extenders, and Pathology Attendants to include workload assignments, training, resolution of technical problems, staffing, scheduling, counseling and other disciplinary measures. Orders supplies, maintains and reviews quality control records and maintains workload statistic data. Regards, Rob Cordero Imaging and Laboratory Sciences Consultant Permanent Placement Division CompHealth Associates, Inc. Phone: 866.782.9029 x 5866 Direct: 954.343.5866 Fax: 800.420.2329 E-mail: robert.cordero@comphealth.com www.comphealth.com About us: http://www.comphealth.com/about_us/about ASK ME ABOUT OUR $250 REFERRAL BONUS Customer service is the key to success. Are you satisfied with your experience? Send your comments to my manager at valerie.patterson@comphealth.com. Please note: "This is a commercial email from CompHealth Associates, Inc. If you do not want to receive future emails from CompHealth Associates, Inc., please reply to the sender of this email and ask to be removed from our list." From gray <@t> rarc.wisc.edu Thu Oct 1 09:16:35 2009 From: gray <@t> rarc.wisc.edu (Beth A Gray) Date: Thu Oct 1 09:16:40 2009 Subject: [Histonet] decal for a seahorse Message-ID: <0FC7CF1C-9998-4E07-A8A5-EB00822E4FF5@rarc.wisc.edu> I need to decal a sea horse. Would rather not use hydrochloric acid. Does anyone have a method that works? Thanks. From isabel.brandao <@t> sarah.br Thu Oct 1 09:19:32 2009 From: isabel.brandao <@t> sarah.br (Isabel Cristina Soares Brandao) Date: Thu Oct 1 09:19:46 2009 Subject: [Histonet] Sakura TDE 30 Message-ID: <60FCAC5244B5D140B2E8FD0DC7F4CCCF190D99@lnmail00.sarah.br> Hi, Does anyone out there have experience with the decalcifier System from Sakura? We have just recived the equipment but we could'nt get the results that they describe in the brochure. Isabel C.S. Brand?o Associa??o das Pioneiras Sociais Patologia Cir?rgica - Bras?lia - Brasil From victor <@t> pathology.washington.edu Thu Oct 1 09:20:06 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Oct 1 09:20:13 2009 Subject: [Histonet] [Fwd: [PPMA] NSH meeting] Barcoding Message-ID: <4AC4BA96.7070407@pathology.washington.edu> For those of you interested in barcoding, this might be a worthwhile talk. Yes, he is my boss so I should put in a plug for him. Victor -------- Original Message -------- Subject: [PPMA] NSH meeting Date: Wed, 30 Sep 2009 17:17:37 -0700 (Pacific Daylight Time) From: Rodney Schmidt Reply-To: A mutual assistance forum for PowerPath users To: PowerPath Mutual Assistance For those of you attending the NSH meeting this next week, I hope to see some of you there. I'll be giving a talk on Tuesday morning about the quality and efficiency benefits of barcoding in the AP lab. If you'd like to get together for dinner or meet some other time, please drop me an off-line email. Unfortunately, my stay in Birmingham is going to be fairly short -- from Monday afternoon until Tuesday afternoon. See you there! Rodney A. Schmidt, M.D., Ph.D. Professor of Pathology Director of Medical Informatics University of Washington, Seattle, WA (206) 598-6462 (206) 344-0532 (pager) (206) 598-3803 (fax) ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ PowerPath Mutual Assistance [PPMA] mailing list PPMA@u.washington.edu http://mailman2.u.washington.edu/mailman/listinfo/ppma -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From kalschev <@t> svm.vetmed.wisc.edu Thu Oct 1 09:45:18 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Thu Oct 1 09:46:13 2009 Subject: [Histonet] decal sea-horse Message-ID: <004e01ca42a5$cb6dfae0$c5d76880@vetmed.wisc.edu> Beth, Citrate Buffered Formic Acid is slow, however, it might work well for this tissue. The recipe is in histology text-books, or contact me. Vicki From jcampbell <@t> vdxpathology.com Thu Oct 1 09:51:49 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Thu Oct 1 09:51:53 2009 Subject: [Histonet] cuisinart pressure cooker Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82AC1E8@VDXSERVER01.vdxpathology.local> Hi all, Does anyone use the cuisinart pressure cooker (with digital display) for their antigen retrieval and what settings do you use? I just purchased one and am about to try it out but, it has a high and low pressure setting, where you are supposed to select the time to pressure cook, and then it has "browning", "saute" and "simmer" settings, which look like pre-cook settings, used with the lid off. I will most likely not use the pre-cook settings I'm assuming. So do you typically select the high pressure setting? And for how long do you pressure cook? Thanks so much, Jennifer From rsrichmond <@t> gmail.com Thu Oct 1 09:56:53 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Oct 1 09:56:56 2009 Subject: [Histonet] Re: discarding old dry chemicals with no expiration date Message-ID: Kelly D. Boyd, BS, HTL (ASCP) at Harris Histology Services in Greenville NC describes having a CLIA inspector ordering that all dry chemicals older than 10 years be discarded. Allen Smith sums it up >>The problem is letting people with little or no knowledge of chemistry make chemical decisions.<>Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo.<< Was this indigo, or was it the indigo-like dyes obtained from shellfish that they used? This remains a lively subject of debate among Jewish scholars. Present day Jews do not dye the fringes (tzitzit) of their prayer shawls (tallit or tallis), thinking that the technique has been lost (a vociferous fringe group - you might say - is using shellfish-derived dyes and has a cool T-shirt - I want one). Bob Richmond Samurai Pathologist Knoxville TN From thomas.crowell <@t> novartis.com Thu Oct 1 10:07:26 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Oct 1 10:07:34 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 10/01/2009 and will not return until 10/06/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. From asmith <@t> mail.barry.edu Thu Oct 1 10:28:56 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Oct 1 10:29:46 2009 Subject: [Histonet] Re: discarding old dry chemicals with no expiration date In-Reply-To: References: Message-ID: The stuff found in the Judean caves is "kal ilan," indigo derived from Indigofera tinctoria. It is chemically identical to "tekhelet," indigo derived from Murex trunculus by photochemical debromination of Tyrian purple. Although some second and third century Jews, knowingly or unknowingly, used kal ilan instead of tekhelet, Rabbinic opinion has generally held that only tekhelet is kosher for dyeing the fringes. However, the Talmud also remarks that, "Only God can tell the difference between tekhelet and kal ilan." Modern spectrometry can detect traces of cellulose in kal ilan, which are not present in tekhelet. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 01, 2009 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: discarding old dry chemicals with no expiration date Kelly D. Boyd, BS, HTL (ASCP) at Harris Histology Services in Greenville NC describes having a CLIA inspector ordering that all dry chemicals older than 10 years be discarded. Allen Smith sums it up >>The problem is letting people with little or no knowledge of chemistry make chemical decisions.<>Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo.<< Was this indigo, or was it the indigo-like dyes obtained from shellfish that they used? This remains a lively subject of debate among Jewish scholars. Present day Jews do not dye the fringes (tzitzit) of their prayer shawls (tallit or tallis), thinking that the technique has been lost (a vociferous fringe group - you might say - is using shellfish-derived dyes and has a cool T-shirt - I want one). Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Thu Oct 1 10:42:16 2009 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Oct 1 10:42:22 2009 Subject: [Histonet] RE:CD3 on mouse tissue In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC0657807BBF201@srvex03.phsabc.ehcnet.ca> Hey John, LabVision (through Thermo Fisher) has an awesome CD3 RbMAb, I use it all the time on both human and mouse FFPE tissue and we have published a few papers on it. Cat# RM-9107, I use it on the Ventana but it also works well for benchtop, I think it's about a 1/150 dilution. If you want to see it on mouse tissue just search for me (milne k) on www.pubmed.com and it should bring up the papers (look for the mouse related ones since 2007 also a couple ovarian cancer ones, there are a couple milne k's out there!), if you don't have access I can e-mail you a PDF but I would strongly stand behind that Ab, it's my favorite and has yet to fail me! Katy ------------------------------ Message: 18 Date: Thu, 1 Oct 2009 08:48:03 -0400 From: John Shelley Subject: [Histonet] CD3 on Mouse Tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Fellow Histonetters, I have been trying to optimize CD3 from Abcam (ab56313) for quite awhile now and I am at my wits end with it. I have tried various retrieval methods and dilutions and polymer kits all to no avail. Has any researcher out there worked with this CD3 before or has a CD3 marker that they found to work and would you be willing to share what you did to get it to work. I have this group breathing down my neck because they wanted their results by last week. Any help would be greatly appreciated, thanks!!! John From sfeher <@t> CMC-NH.ORG Thu Oct 1 11:02:29 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Oct 1 11:02:33 2009 Subject: [Histonet] LEAN at NSH Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1FEB@exchange.cmc-nh.org> For any of you that are attending NSH in Birmingham and interested in the practical applications of LEAN, I will, along with a professional work flow analyst, be giving a workshop (WS # 8) on Saturday morning. The design is to give a basic overview of LEAN, demonstrate how the principles of LEAN were used in designing a new Pathology lab, and an exercise where the attendees will have the opportunity to design their own unique Path Lab using LEAN principles. It will be a great way to see how this catch phrase everyone is using these days can be put in to practice. Hope to see you there. Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From alyssa <@t> alliedsearchpartners.com Thu Oct 1 11:09:50 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Oct 1 11:09:56 2009 Subject: [Histonet] Part Time Job In FL Message-ID: One of my clients has asked me to do a favor and spread the word about a Part Time Moh's histotechnician/histotechnologist position in Altamonte Springs, FL with a private laboratory. Please let me know if you or anyone that you know would be interested in the following position: Position: Histotechnologist/Histotechnician Time: 8am-4pm Days:Mondays Only Facility: Private Derm Lab Location: Altamonte Springs, FL Description: Preforms Moh's Histology Please submit resume to alyssa@alliedsearchpartners.com for initial prescreening, and one of our recruiters will follow up with a phone screen/interview after receiving your resume. Have a great day! -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From jcampbell <@t> vdxpathology.com Thu Oct 1 11:35:24 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Thu Oct 1 11:35:30 2009 Subject: [Histonet] Is anyone familiar with Granzyme B antibody? Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82AC20E@VDXSERVER01.vdxpathology.local> I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer From Marilyn.A.Weiss <@t> kp.org Thu Oct 1 12:00:38 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Oct 1 12:00:52 2009 Subject: [Histonet] marilyn weiss will be out of the office as of 5 a.m.10/1/09 Message-ID: I will be out of the office starting 10/01/2009 and will not return until 10/05/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. From louise.renton <@t> gmail.com Thu Oct 1 12:35:26 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Oct 1 12:35:34 2009 Subject: [Histonet] Sakura TDE 30 In-Reply-To: <60FCAC5244B5D140B2E8FD0DC7F4CCCF190D99@lnmail00.sarah.br> References: <60FCAC5244B5D140B2E8FD0DC7F4CCCF190D99@lnmail00.sarah.br> Message-ID: Could you please explain what the problems are. I have had excellent results with this system On 10/1/09, Isabel Cristina Soares Brandao wrote: > Hi, > Does anyone out there have experience with the decalcifier System from > Sakura? We have just recived the equipment but we could'nt get the results > that they describe in the brochure. > Isabel C.S. Brand?o > Associa??o das Pioneiras Sociais > Patologia Cir?rgica - Bras?lia - Brasil > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From kmilne <@t> bccancer.bc.ca Thu Oct 1 12:57:27 2009 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Oct 1 12:57:33 2009 Subject: [Histonet] RE: Is anyone familiar with Granzyme B antibody? In-Reply-To: <9cf45b97-574b-4b22-aede-4a6eae59e8be@SRVEXHT02.phsabc.ehcnet.ca> References: <9cf45b97-574b-4b22-aede-4a6eae59e8be@SRVEXHT02.phsabc.ehcnet.ca> Message-ID: <07979E76B0869D4E8C9FE4AA9FC0657807BBF20D@srvex03.phsabc.ehcnet.ca> Wow, is it my day for familiar Abs or what?! We've used Abcam's Granzyme B (cat #ab4059) for both our mouse and human FFPE work. It works quite well, it's a RbPAb at 1/50. Let me know if you'd like me to forward the paper we used it on. Katy Message: 9 Date: Thu, 1 Oct 2009 09:35:24 -0700 From: "Jennifer Campbell" Subject: [Histonet] Is anyone familiar with Granzyme B antibody? To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82AC20E@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 71, Issue 2 *************************************** From carmen_loiselle <@t> hotmail.com Thu Oct 1 13:23:49 2009 From: carmen_loiselle <@t> hotmail.com (carmen loiselle) Date: Thu Oct 1 13:23:53 2009 Subject: [Histonet] SOP's reference Message-ID: Hello everyone, I've been assigned recently to prepare the SOP's for the histology laboratory for the accreditation of my institution that englobes 4 major hospitals, that will take place next september (2010). As you well know it's a very demanding and time consuming task, so I'm asking your help, once again. If anyone will be able to send me the best sites where I can find the informations about different stains , like purpose , objectives etc , in order to facilitate the production of all these SOP's . Specially that I'm doing it only part time. Any info or references would be greatly appreciated. Thanks in advance Carmy _________________________________________________________________ Nous sommes vos photos. Partagez-nous d?s maintenant avec Windows Live Photos. http://go.microsoft.com/?linkid=9666051 From DKBoyd <@t> chs.net Thu Oct 1 13:40:29 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Oct 1 13:40:26 2009 Subject: [Histonet] SOP's reference In-Reply-To: Message-ID: Freda Carson; Histotechnology A Self Instruction Text. Sheehan Hrapchak: Theory and practice of Histotechnology Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net carmen loiselle Sent by: histonet-bounces@lists.utsouthwestern.edu 10/01/2009 02:24 PM To pathology education cc Subject [Histonet] SOP's reference Hello everyone, I've been assigned recently to prepare the SOP's for the histology laboratory for the accreditation of my institution that englobes 4 major hospitals, that will take place next september (2010). As you well know it's a very demanding and time consuming task, so I'm asking your help, once again. If anyone will be able to send me the best sites where I can find the informations about different stains , like purpose , objectives etc , in order to facilitate the production of all these SOP's . Specially that I'm doing it only part time. Any info or references would be greatly appreciated. Thanks in advance Carmy _________________________________________________________________ Nous sommes vos photos. Partagez-nous d?s maintenant avec Windows Live Photos. http://go.microsoft.com/?linkid=9666051_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From talulahgosh <@t> gmail.com Thu Oct 1 14:05:02 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Oct 1 14:05:09 2009 Subject: [Histonet] paraffin sectioning static Message-ID: Any tips on getting rid of static when paraffin sectioning on a microtome? It's so insanely bad, the block is picking up the ribbon as it goes back up every third section. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum From LSebree <@t> uwhealth.org Thu Oct 1 14:21:01 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Oct 1 14:21:06 2009 Subject: [Histonet] paraffin sectioning static In-Reply-To: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A589035706F4@UWHC-MAIL01.uwhis.hosp.wisc.edu> The things we've tried Emily are: rubbing a gauze soaked with Static Gard on and around the microtome, brushing the microtome with a Static Master brush, and/or laying down wet gauze around your blade holder. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, October 01, 2009 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin sectioning static Any tips on getting rid of static when paraffin sectioning on a microtome? It's so insanely bad, the block is picking up the ribbon as it goes back up every third section. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Thu Oct 1 14:23:29 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Oct 1 14:23:50 2009 Subject: [Histonet] paraffin sectioning static Message-ID: Heavy breathing- the pathologist walking by might do a double-take, but the humidy from your breath should get rid of immediate static. Alternatively use a humidifier in the lab, but of course that comes with its own set of problems (ie aerosolizing Pseudomonas and other environmental bacteria and/or fungii). Just a thought (and a little humour for good measure!). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Emily Sours 10/1/2009 4:05 PM >>> Any tips on getting rid of static when paraffin sectioning on a microtome? It's so insanely bad, the block is picking up the ribbon as it goes back up every third section. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From carl.hobbs <@t> kcl.ac.uk Thu Oct 1 14:25:31 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Oct 1 14:25:46 2009 Subject: [Histonet] RE:CD3 on mouse tissue Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742AD7@KCL-MAIL04.kclad.ds.kcl.ac.uk> I assume FFPW sections? I agree that exLabvision's Neomarkeranti CD3 is superb. So is Dako's anti CD3, imho. Is Dako still owned by...Dako? Assuming that these two Abs give identical staining patterns, which of the two are most cost-effective? I would be very interested in opinions. Regards/Best wishes/Best regards, Sincerely....which is best? Other languages that we could/should use? Or....just stick to Name? Carl If you wish to have a look at more images of anti CD3 results on FFPW sections: http://www.immunoportal.com/index.ph From Jackie.O'Connor <@t> abbott.com Thu Oct 1 14:25:55 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Oct 1 14:26:22 2009 Subject: [Histonet] paraffin sectioning static In-Reply-To: <5998F3BDFF7AAC4091C7AE93A7A1A589035706F4@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <5998F3BDFF7AAC4091C7AE93A7A1A589035706F4@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: Wipe over the microtome and the immediate area with a dryer sheet. Jackie O' From: "Sebree Linda A" To: "Emily Sours" , Date: 10/01/2009 02:21 PM Subject: RE: [Histonet] paraffin sectioning static Sent by: histonet-bounces@lists.utsouthwestern.edu The things we've tried Emily are: rubbing a gauze soaked with Static Gard on and around the microtome, brushing the microtome with a Static Master brush, and/or laying down wet gauze around your blade holder. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, October 01, 2009 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin sectioning static Any tips on getting rid of static when paraffin sectioning on a microtome? It's so insanely bad, the block is picking up the ribbon as it goes back up every third section. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Oct 1 14:28:29 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 1 14:28:34 2009 Subject: [Histonet] paraffin sectioning static In-Reply-To: <5998F3BDFF7AAC4091C7AE93A7A1A589035706F4@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <5998F3BDFF7AAC4091C7AE93A7A1A589035706F4@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: Dryer sheets work well too - wipe the microtome down - and your clothes and hands.. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, October 01, 2009 15:21 To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] paraffin sectioning static The things we've tried Emily are: rubbing a gauze soaked with Static Gard on and around the microtome, brushing the microtome with a Static Master brush, and/or laying down wet gauze around your blade holder. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, October 01, 2009 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin sectioning static Any tips on getting rid of static when paraffin sectioning on a microtome? It's so insanely bad, the block is picking up the ribbon as it goes back up every third section. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Thu Oct 1 14:51:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 1 14:52:03 2009 Subject: [Histonet] SOP's reference In-Reply-To: Message-ID: <779299.34643.qm@web65710.mail.ac4.yahoo.com> Any good histotechniques book will provide all the information you need. Check Dr. Kiernan's Ren? J. --- On Thu, 10/1/09, carmen loiselle wrote: From: carmen loiselle Subject: [Histonet] SOP's reference To: "pathology education" Date: Thursday, October 1, 2009, 2:23 PM Hello everyone, I've been assigned recently to prepare the SOP's for the histology laboratory for the accreditation of my institution that englobes 4 major hospitals, that will take place next september (2010).? As you well know it's a very demanding and time consuming task, so I'm asking your help, once again.? If anyone will be able to send me the best sites where I can find the informations about different stains , like purpose , objectives etc , in order to facilitate the production of all these SOP's .? Specially that I'm doing it only part time. Any info or references would be greatly appreciated. Thanks in advance Carmy ??? ???????? ?????? ??? ? _________________________________________________________________ Nous sommes vos photos. Partagez-nous d?s maintenant avec Windows Live Photos. http://go.microsoft.com/?linkid=9666051_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Thu Oct 1 17:40:06 2009 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Thu Oct 1 17:40:13 2009 Subject: [Histonet] Is anyone familiar with Granzyme B antibody? In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF82AC20E@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF82AC20E@VDXSERVER01.vdxpathology.local> Message-ID: <448918.3076.qm@web83915.mail.sp1.yahoo.com> Unfortunately I am trying to get out of the lab and go home to pack for the NSH, so I do not have the catalog number, but eBioscinece has a good?biotin anti mouse?Granzyme B cat#13-8822 that works well on mouse tissue. They also have a biotin anti mouse F4/80. Good luck Dusko ________________________________ From: Jennifer Campbell To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 1, 2009 9:35:24 AM Subject: [Histonet] Is anyone familiar with Granzyme B antibody? ? I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue.? Does anyone have any vendors they could recommend and/or protocols?? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Oct 2 02:16:53 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Oct 2 02:17:18 2009 Subject: [Histonet] Sakura TDE 30 In-Reply-To: <60FCAC5244B5D140B2E8FD0DC7F4CCCF190D9D@lnmail00.sarah.br> References: <60FCAC5244B5D140B2E8FD0DC7F4CCCF190D9D@lnmail00.sarah.br> Message-ID: OK, Firstly, what you do depends on what samples you are dealing with. We decalcify smallish samples of bone about 1x1cm - this can either compact or trabecular bone. Here are some of the things I learned...... 1. Do not overload the chamber.....place samples between the electrodes, and rather do fewer samples - decal can be very quick if used right. 2. Do not use metal lids on casettes if using them (or any metal clips, parts etc) 3. If the samples aer heavily mineralised, ie huge pieces of compact bone, change the fluid when you see that the fizzing around the electrodes has slowed 4. Rather swich off the unit overnight (with the samples still in) if you are doing a long decal - then swich on in the day, so that you can monitor progress. 5. Not all sam[les will decalcify at the same rate - check them all individually at 4 - 5hr, or in the morning of the next day 5. When decal is finished - rinse samples thoroughly in running tap water for at LEAST and hour before processing 6. I have decalcified a slice of pig femur about 0.5 cm thick in about 5hrs hope this helps On Thu, Oct 1, 2009 at 7:48 PM, Isabel Cristina Soares Brandao < isabel.brandao@sarah.br> wrote: > The time expended in the process was too long and the tissues had artifacts > when examined on the microscope. We did exactly the same procedure described > in the Sakura brochure. > Do you do something different? How do you verify the process? How long does > it take to get the samples decalcified? > > Thanks for your help! > > > ---------- > > De: histonet-bounces@lists.utsouthwestern.edu[ > SMTP:histonet-bounces@lists.utsouthwestern.edu] > em nome de louise renton[SMTP:louise.renton@gmail.com > ] > > Enviada: quinta-feira, 1 de outubro de 2009 14:35 > > Para: Histonet@lists.utsouthwestern.edu > > Assunto: Re: [Histonet] Sakura TDE 30 > > > > Could you please explain what the problems are. I have had excellent > results > > with this system > > > > On 10/1/09, Isabel Cristina Soares Brandao > wrote: > > > > > Hi, > > > Does anyone out there have experience with the decalcifier System from > > > Sakura? We have just recived the equipment but we could'nt get the > results > > > that they describe in the brochure. > > > Isabel C.S. Brand?o > > > Associa??o das Pioneiras Sociais > > > Patologia Cir?rgica - Bras?lia - Brasil > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > -- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From tahseen <@t> brain.net.pk Fri Oct 2 03:19:52 2009 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Fri Oct 2 03:20:01 2009 Subject: [Histonet] (no subject) Message-ID: <21820.202.125.145.178.1254471592.squirrel@brain.net.pk> Hi All, I used Monoclonal Mouse Antibody to insulin for the localization of of insulin positive beta cells in islets of wistar rats and for viaualization I used by Envision (HRP). IHC staining was stongly positive in human islets with this technique where as in rodents staining was negative even in positive control rats.Kindly guide me regarding the staining faliure in my case what would the possible cause? Awaiting for your earliest reply. Best Regards Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan From wmondy <@t> verizon.net Fri Oct 2 06:35:37 2009 From: wmondy <@t> verizon.net (William Lafayette Mundey) Date: Fri Oct 2 06:36:01 2009 Subject: [Histonet] Basment membranes In-Reply-To: <448918.3076.qm@web83915.mail.sp1.yahoo.com> References: <5658CBDB9EAE6545ABE50D2563D81BF82AC20E@VDXSERVER01.vdxpathology.local> <448918.3076.qm@web83915.mail.sp1.yahoo.com> Message-ID: <000901ca4354$76ba38b0$642eaa10$@net> Does anyone know any stains Specific for basement membrane? From DKBoyd <@t> chs.net Fri Oct 2 06:50:49 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Oct 2 06:50:40 2009 Subject: [Histonet] Basement membranes In-Reply-To: <000901ca4354$76ba38b0$642eaa10$@net> Message-ID: Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "William Lafayette Mundey" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmondy@verizon.net To cc Subject [Histonet] Basment membranes Does anyone know any stains Specific for basement membrane? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From gokland <@t> ameripath.com Fri Oct 2 06:49:32 2009 From: gokland <@t> ameripath.com (Okland, Gloria) Date: Fri Oct 2 06:56:03 2009 Subject: [Histonet] Amoeba Control Message-ID: <6240949703BA0B4FB724CF6B404BC3F10305D0FDC5@MWNMAIL00.ameripath.local> I am in need of an Amoeba control. Does anyone know of a vendor that would have these in stock, or other suggestions for finding one? Thank you very much. Gloria From wmondy <@t> verizon.net Fri Oct 2 08:22:59 2009 From: wmondy <@t> verizon.net (William Lafayette Mundey) Date: Fri Oct 2 08:23:24 2009 Subject: [Histonet] Basement membranes In-Reply-To: References: <000901ca4354$76ba38b0$642eaa10$@net> Message-ID: <001901ca4363$764cfa20$62e6ee60$@net> Can the Periodic Acid-Methenamine Silver Procedure for Basement Membranes or the Jones be done in block? From: DKBoyd@chs.net [mailto:DKBoyd@chs.net] Sent: Friday, October 02, 2009 7:51 AM To: wmondy@verizon.net Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement membranes Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "William Lafayette Mundey" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmondy@verizon.net To cc Subject [Histonet] Basment membranes Does anyone know any stains Specific for basement membrane? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From b-frederick <@t> northwestern.edu Fri Oct 2 08:29:20 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Oct 2 08:29:32 2009 Subject: [Histonet] Basement membranes In-Reply-To: Message-ID: <67ED01BC5D704FF984F839538E86C3F1@lurie.northwestern.edu> AKA Jones Method. Sigma sells a kit or you can make it all up yourself. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Friday, October 02, 2009 6:51 AM To: wmondy@verizon.net Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement membranes Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "William Lafayette Mundey" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmondy@verizon.net To cc Subject [Histonet] Basment membranes Does anyone know any stains Specific for basement membrane? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 2 08:32:42 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 2 08:32:47 2009 Subject: [Histonet] Basement membranes In-Reply-To: <001901ca4363$764cfa20$62e6ee60$@net> Message-ID: <721694.95032.qm@web65712.mail.ac4.yahoo.com> No, just in sections. Ren? J. --- On Fri, 10/2/09, William Lafayette Mundey wrote: From: William Lafayette Mundey Subject: RE: [Histonet] Basement membranes To: DKBoyd@chs.net Cc: histonet@lists.utsouthwestern.edu Date: Friday, October 2, 2009, 9:22 AM Can the Periodic Acid-Methenamine Silver Procedure for Basement Membranes or the Jones be done in block? From: DKBoyd@chs.net [mailto:DKBoyd@chs.net] Sent: Friday, October 02, 2009 7:51 AM To: wmondy@verizon.net Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement membranes Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane? showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va.? 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "William Lafayette Mundey" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmondy@verizon.net To cc ??? Subject [Histonet] Basment membranes ??? ??? Does anyone know any stains Specific for basement membrane? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From alyssa <@t> alliedsearchpartners.com Fri Oct 2 09:44:46 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Oct 2 09:44:50 2009 Subject: [Histonet] Job Opening-Cytopathology Fellow Message-ID: Allied Search Partners is now accepting resumes for one of our client's in Phoenix, AZ. We are accepting resumes for a *Cytopathology Fellow.* Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* Once resume is submitted one of our recruiters will follow up with you for a phone screen. No resume will be submitted to our client until we speak to you. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. Job Description: Cytopathology Fellowship Requirements: Board certification Fine needle aspiration experiences a plus -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From saby_joseph_a <@t> yahoo.com Fri Oct 2 10:36:28 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Fri Oct 2 10:36:32 2009 Subject: [Histonet] Eager's Method Message-ID: <519295.13107.qm@web112614.mail.gq1.yahoo.com> Fellow Histonetters- I could use some help. I have a request to use a stain for sympathetic nerve fibers called Eager's that can be combined with Luxol Fast Blue / Crsyl Echt Violet. I know many of you would recommend GFAP or S100, and you would be absolutely correct.? Unfortunately, that is not on the table here. I thank in advance any of you who respond with the reference?I need. Joe Saby, BA HT From o.isaac24 <@t> yahoo.com Fri Oct 2 12:16:51 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Fri Oct 2 12:17:00 2009 Subject: [Histonet] Fw: IHC POSITION WANTED Message-ID: <252409.15072.qm@web111611.mail.gq1.yahoo.com> ----- Forwarded Message ---- From: Isaac O To: histonet@lists.utsouthwestern.edu Sent: Wednesday, September 23, 2009 6:55:49 PM Subject: IHC POSITION WANTED Hi, ?? I am looking for a new IHC position. I am HTL(ASCP) certified. I am open to relocation. ?Isaac. From jcarpenter764 <@t> aol.com Fri Oct 2 16:25:32 2009 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Fri Oct 2 16:25:50 2009 Subject: [Histonet] Does anyone have a procedure for the new Telepathology and digital imaging questions in the CAP questionnaire. Message-ID: <8CC11BE018E0EE8-2DBC-35719@webmail-m070.sysops.aol.com> Does anyone have a procedure for the new Telepathology and digital imaging questions in the CAP questionnaire? Thanks. From abright <@t> brightinstruments.com Fri Oct 2 17:04:45 2009 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Oct 2 17:05:55 2009 Subject: [Histonet] Histobath Frozen Sections In-Reply-To: <9946FFA3E7ABAF489D048D0F8EA4755404803359@VHAV16MSGA1.V16.Med.VA.Gov> References: <9946FFA3E7ABAF489D048D0F8EA4755404803359@VHAV16MSGA1.V16.Med.VA.Gov> Message-ID: <1529327132-1254521078-cardhu_decombobulator_blackberry.rim.net-670920445-@bda177.bisx.produk.on.blackberry> Dear Deborah, Yes we can help you on this, we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. C. Please see this model on www.brightinstruments.com Best Regards Alan Bright Sent from my BlackBerry? wireless device -----Original Message----- From: "Edwards, Deborah K" Date: Wed, 30 Sep 2009 15:04:00 To: Subject: [Histonet] Histobath Frozen Sections Hello, I am looking for a replacement for my Shandon Histo-bath. It is a snap freeze instrument that I have been using with Isopentane. Do you have something comparable to this or any suggestion? My Histobath finally gave up. Many thanks, Deborah K. Edwards, HTL/QIHC, (ASCP) Supervisor of Histology MEDVAMC Lab 113 2002 Holcombe Houston, Texas 77030 713-794-7259 (v.m.) e-mail: Edwards.deborahk@va.gov 713-794-7260 (histology lab) 713-794-7657 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ardavis <@t> wlgore.com Fri Oct 2 18:00:51 2009 From: ardavis <@t> wlgore.com (Araceli Davis) Date: Fri Oct 2 18:00:58 2009 Subject: [Histonet] Araceli Davis is out of the office will return 10-8-09 Message-ID: I will be out of the office starting 10/02/2009 and will not return until 10/09/2009. Any histology request contact Histology@WLGore.com From pruegg <@t> ihctech.net Sat Oct 3 09:47:15 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Oct 3 09:47:57 2009 Subject: SPAM-LOW: [Histonet] (no subject) In-Reply-To: <21820.202.125.145.178.1254471592.squirrel@brain.net.pk> References: <21820.202.125.145.178.1254471592.squirrel@brain.net.pk> Message-ID: Most likely the ab is mouse anti human and does not cross react to mouse beta cells. Another issue could be since you are using a mouse antibody on mouse tissue I assume you have blocked to prevent non specific binding of the mouse Ig ab to endogenous mouse Ig? You may have blocked too well. If you did not block to avoid mouse on mouse binding of Ig from the anti mouse detection then this does not apply. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk Sent: Friday, October 02, 2009 2:20 AM To: histonet@lists.utsouthwestern.edu Cc: localization of of Subject: SPAM-LOW: [Histonet] (no subject) Hi All, I used Monoclonal Mouse Antibody to insulin for the localization of of insulin positive beta cells in islets of wistar rats and for viaualization I used by Envision (HRP). IHC staining was stongly positive in human islets with this technique where as in rodents staining was negative even in positive control rats.Kindly guide me regarding the staining faliure in my case what would the possible cause? Awaiting for your earliest reply. Best Regards Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotemp <@t> yahoo.com Sun Oct 4 22:39:00 2009 From: histotemp <@t> yahoo.com (Scott Gill) Date: Sun Oct 4 22:39:04 2009 Subject: [Histonet] Desperately need frozen kidney Message-ID: <573555.19370.qm@web58303.mail.re3.yahoo.com> Hello, ? My research project is in desperate need of a couple fresh frozen pieces of kidney positive for C3 and/or C4.? (lupus?)? I will gladly pay any costs to get the tissue sent.? (boxes, dry ice, postage, etc.) ? Thanks in advance for your time Scott histotemp@yahoo.com.? From annigyg <@t> gmail.com Sun Oct 4 23:38:06 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sun Oct 4 23:38:11 2009 Subject: [Histonet] Brain Frozen sections Message-ID: Hi Histonetters we are having INTERMITTENT problems with brain frozens all conditions are the same ie. same tech, same cryostat, same freezing technique - sometimes there is freezing artefact in the sections and sometimes the sections look like they were cut from an FFPE block!! unfortunately not all our pathologists are confident to make an IOC diagnosis on a smears so we are forced to resort to freezing. my tech is very experieince and says she can almost predict when looking at the tissue, that there will be artefact on the sections. pathologists are insisting on the FZ but complaining bitterly at the freezing artefact. I suspect the surgeon may be 'flooding' the tissue with saline but unless we breathe down their necks we cannot possibly know what goes on in the OR! All other frozens are fine - its just the very small neuro ones we are having a problem with and it is driving us to distraction - well, actually the Pathologists are doing that!! we are using cryospray, gently sprayed onto the tissue, which is covered with a small drop of fresh OCT, on top of an already frozen button of OCT which is kept in the cryostat there is no problem with other frozen sections, on other tissues, prepared in exactly the same way any and all observations/recommendations/suggestions will be greatfully accepted TIA -- Anne van Binsbergen (Hope) Abu Dhabi UAE From joseph-galbraith <@t> uiowa.edu Mon Oct 5 09:16:41 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Oct 5 09:16:44 2009 Subject: [Histonet] Histobath Frozen Sections In-Reply-To: References: Message-ID: Melanie: We are acquiring a SnapFrost 2 unit from Alphelys. They offer two models - one at -80 and one at -100. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Wednesday, September 23, 2009 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath Frozen Sections Histonetters: In our sister lab, the Histobath freezing unit has died. We need to know if there is another comparable product available, since the Histobath is no longer manufactured. Our pathologists don't care for the peltier option. We researched the archives and found that this has been discussed on the Histonet in the past, but didn't see many responses. There is a similar (but larger, if I understand correctly) unit available from a vendor in the UK. Can anyone contribute suggestions or comments? Thanks in advance. P.S. Are there any other Samurai Pathologists available? We need one. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Mon Oct 5 10:06:31 2009 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Oct 5 10:06:39 2009 Subject: [Histonet] time in paraffin and fried bloody specimen Message-ID: <737BD0BF52F0744B96B74B61756AC0644145E938BF@hestia.ad.pa-ucl.com> Good Morning Histonetters- First question: Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From plucas <@t> biopath.org Mon Oct 5 10:20:06 2009 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon Oct 5 10:20:10 2009 Subject: [Histonet] Leica Bond/Novocastra Reagents Message-ID: <20091005152006.93D578882@arkroyal.cnchost.com> Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA From JCBRITTON <@t> Cheshire-Med.COM Mon Oct 5 10:29:12 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Mon Oct 5 10:29:18 2009 Subject: [Histonet] Leica Bond/Novocastra Reagents In-Reply-To: <20091005152006.93D578882@arkroyal.cnchost.com> References: <20091005152006.93D578882@arkroyal.cnchost.com> Message-ID: We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From MAUGER <@t> email.chop.edu Mon Oct 5 10:41:44 2009 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Mon Oct 5 10:42:29 2009 Subject: [Histonet] Leica Bond/Novocastra Reagents In-Reply-To: References: <20091005152006.93D578882@arkroyal.cnchost.com> Message-ID: <4AC9DB780200003100014828@email.chop.edu> We have never had a backorder problem- have 2 Bondmax's over 2 years. I love them. Jo Mauger >>> "Josie Britton" 10/05/09 11:29 AM >>> We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Mon Oct 5 10:48:45 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Oct 5 10:49:08 2009 Subject: [Histonet] Leica Bond/Novocastra Reagents Message-ID: Almost always receive on time. I can think of only one instance in a year and a half where anything was on back order (it was an antibody). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Paula Lucas 10/5/2009 12:20 PM >>> Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From JWeems <@t> sjha.org Mon Oct 5 10:49:35 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 5 10:49:35 2009 Subject: [Histonet] Leica Bond/Novocastra Reagents In-Reply-To: <20091005152006.93D578882@arkroyal.cnchost.com> References: <20091005152006.93D578882@arkroyal.cnchost.com> Message-ID: We've had no problems with either Bond or Novacastra orders. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Mon Oct 5 11:04:27 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 5 11:04:31 2009 Subject: [Histonet] time in paraffin and fried bloody specimen In-Reply-To: <737BD0BF52F0744B96B74B61756AC0644145E938BF@hestia.ad.pa-ucl.com> Message-ID: <345606.14124.qm@web65711.mail.ac4.yahoo.com> After you have developed a processing protocol and?obtained good infiltration after a certain time (hours) in paraffin, any and all the time above that period of adequate infiltration = exposure to prolonged heat. Some histotechs even don't fill the holding chamber in the embedding center, a practice I do not think is adequate. To your second question, just place them in NBF and when fixed filter and wrap them yourself while cassetting, do not wrap them before being fixed. Ren? J. --- On Mon, 10/5/09, Nancy Schmitt wrote: From: Nancy Schmitt Subject: [Histonet] time in paraffin and fried bloody specimen To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Monday, October 5, 2009, 11:06 AM Good Morning Histonetters- First question:? Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening".? Can anyone define "exposure to prolonged heat"?? Is that an hour? Three hours?? Sitting in the paraffin waiting to be drained.? I would appreciate some insight on this. Second question:? Endom, POC tissue, even some sinus contents arrive wrapped in lens paper.? These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper.? Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From DKBoyd <@t> chs.net Mon Oct 5 11:11:54 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Oct 5 11:11:41 2009 Subject: [Histonet] time in paraffin and fried bloody specimen In-Reply-To: <737BD0BF52F0744B96B74B61756AC0644145E938BF@hestia.ad.pa-ucl.com> Message-ID: Nancy, Tissue should be processed @ between 60-62 degrees centigrade. We have three paraffin baths. The 1st bath is set for 45 mins, the 2cd and 3rd are for 1 hour each. This is for large specimens. Small specimens are for 30 mins. the first two baths and 45 mins for the last. It is very true that too much time in paraffin causes hard tissue. Remember the whole time the tissue is setting in paraffin it is being exposed to heat. Your second question: Have the specimen transferred from the lens paper it arrived in and put on a new piece which has been moistened with formalin. Sometimes in surgery the lens paper is wet with saline. If it is a scant amount process with your Endoscopic biopsies. Too long in your alcohols will over dehydrate the specimen. Hope this helps. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Nancy Schmitt Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2009 11:07 AM To "Histonet (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] time in paraffin and fried bloody specimen Good Morning Histonetters- First question: Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rhessler <@t> pathgroup.com Mon Oct 5 12:21:00 2009 From: rhessler <@t> pathgroup.com (Richard Hessler, M.D.) Date: Mon Oct 5 12:21:08 2009 Subject: [Histonet] Brain Frozen sections In-Reply-To: <66891487-460b-41e2-810d-4aaec4512a82@PGNEDGE.pathgroup.com> References: <66891487-460b-41e2-810d-4aaec4512a82@PGNEDGE.pathgroup.com> Message-ID: <197CD0B02A81F94994A285C59C8AE05C04FF87CDBF@pgnexchange.pathgroup.com> There is nothing you can do to make brain frozen sections acceptable. Your Pathologists need to learn how to read smears, or just accept being wrong 50% of the time. An educated guess based on the imaging is more accurate than frozen sections on intra-axial primary brain tumors. Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, October 05, 2009 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 71, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Desperately need frozen kidney (Scott Gill) 2. Brain Frozen sections (Anne van Binsbergen) 3. RE: Histobath Frozen Sections (Galbraith, Joe) 4. time in paraffin and fried bloody specimen (Nancy Schmitt) 5. Leica Bond/Novocastra Reagents (Paula Lucas) 6. RE: Leica Bond/Novocastra Reagents (Josie Britton) 7. RE: Leica Bond/Novocastra Reagents (Joanne Mauger) 8. Re: Leica Bond/Novocastra Reagents (Greg Dobbin) 9. RE: Leica Bond/Novocastra Reagents (Weems, Joyce) 10. Re: time in paraffin and fried bloody specimen (Rene J Buesa) 11. Re: time in paraffin and fried bloody specimen (DKBoyd@chs.net) ---------------------------------------------------------------------- Message: 1 Date: Sun, 4 Oct 2009 20:39:00 -0700 (PDT) From: Scott Gill Subject: [Histonet] Desperately need frozen kidney To: histonet@lists.utsouthwestern.edu Message-ID: <573555.19370.qm@web58303.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, ? My research project is in desperate need of a couple fresh frozen pieces of kidney positive for C3 and/or C4.? (lupus?)? I will gladly pay any costs to get the tissue sent.? (boxes, dry ice, postage, etc.) ? Thanks in advance for your time Scott histotemp@yahoo.com.? ------------------------------ Message: 2 Date: Mon, 5 Oct 2009 08:38:06 +0400 From: Anne van Binsbergen Subject: [Histonet] Brain Frozen sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters we are having INTERMITTENT problems with brain frozens all conditions are the same ie. same tech, same cryostat, same freezing technique - sometimes there is freezing artefact in the sections and sometimes the sections look like they were cut from an FFPE block!! unfortunately not all our pathologists are confident to make an IOC diagnosis on a smears so we are forced to resort to freezing. my tech is very experieince and says she can almost predict when looking at the tissue, that there will be artefact on the sections. pathologists are insisting on the FZ but complaining bitterly at the freezing artefact. I suspect the surgeon may be 'flooding' the tissue with saline but unless we breathe down their necks we cannot possibly know what goes on in the OR! All other frozens are fine - its just the very small neuro ones we are having a problem with and it is driving us to distraction - well, actually the Pathologists are doing that!! we are using cryospray, gently sprayed onto the tissue, which is covered with a small drop of fresh OCT, on top of an already frozen button of OCT which is kept in the cryostat there is no problem with other frozen sections, on other tissues, prepared in exactly the same way any and all observations/recommendations/suggestions will be greatfully accepted TIA -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 3 Date: Mon, 5 Oct 2009 09:16:41 -0500 From: "Galbraith, Joe" Subject: RE: [Histonet] Histobath Frozen Sections To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Melanie: We are acquiring a SnapFrost 2 unit from Alphelys. They offer two models - one at -80 and one at -100. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Wednesday, September 23, 2009 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath Frozen Sections Histonetters: In our sister lab, the Histobath freezing unit has died. We need to know if there is another comparable product available, since the Histobath is no longer manufactured. Our pathologists don't care for the peltier option. We researched the archives and found that this has been discussed on the Histonet in the past, but didn't see many responses. There is a similar (but larger, if I understand correctly) unit available from a vendor in the UK. Can anyone contribute suggestions or comments? Thanks in advance. P.S. Are there any other Samurai Pathologists available? We need one. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 5 Oct 2009 10:06:31 -0500 From: Nancy Schmitt Subject: [Histonet] time in paraffin and fried bloody specimen To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <737BD0BF52F0744B96B74B61756AC0644145E938BF@hestia.ad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Good Morning Histonetters- First question: Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 5 Date: Mon, 05 Oct 2009 08:20:06 -0700 (PDT) From: Paula Lucas Subject: [Histonet] Leica Bond/Novocastra Reagents To: Message-ID: <20091005152006.93D578882@arkroyal.cnchost.com> Content-Type: text/plain; charset="utf-8" Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ------------------------------ Message: 6 Date: Mon, 5 Oct 2009 11:29:12 -0400 From: "Josie Britton" Subject: RE: [Histonet] Leica Bond/Novocastra Reagents To: "Paula Lucas" , Message-ID: Content-Type: text/plain; charset="us-ascii" We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ------------------------------ Message: 7 Date: Mon, 05 Oct 2009 11:41:44 -0400 From: "Joanne Mauger" Subject: RE: [Histonet] Leica Bond/Novocastra Reagents To: ,, Message-ID: <4AC9DB780200003100014828@email.chop.edu> Content-Type: text/plain; charset=US-ASCII We have never had a backorder problem- have 2 Bondmax's over 2 years. I love them. Jo Mauger >>> "Josie Britton" 10/05/09 11:29 AM >>> We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 05 Oct 2009 12:48:45 -0300 From: "Greg Dobbin" Subject: Re: [Histonet] Leica Bond/Novocastra Reagents To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Almost always receive on time. I can think of only one instance in a year and a half where anything was on back order (it was an antibody). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Paula Lucas 10/5/2009 12:20 PM >>> Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ------------------------------ Message: 9 Date: Mon, 5 Oct 2009 11:49:35 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Leica Bond/Novocastra Reagents To: Message-ID: Content-Type: text/plain; charset="us-ascii" We've had no problems with either Bond or Novacastra orders. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 10 Date: Mon, 5 Oct 2009 09:04:27 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] time in paraffin and fried bloody specimen To: "Histonet \(histonet@lists.utsouthwestern.edu\)" , Nancy Schmitt Message-ID: <345606.14124.qm@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 After you have developed a processing protocol and?obtained good infiltration after a certain time (hours) in paraffin, any and all the time above that period of adequate infiltration = exposure to prolonged heat. Some histotechs even don't fill the holding chamber in the embedding center, a practice I do not think is adequate. To your second question, just place them in NBF and when fixed filter and wrap them yourself while cassetting, do not wrap them before being fixed. Ren? J. --- On Mon, 10/5/09, Nancy Schmitt wrote: From: Nancy Schmitt Subject: [Histonet] time in paraffin and fried bloody specimen To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Monday, October 5, 2009, 11:06 AM Good Morning Histonetters- First question:? Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening".? Can anyone define "exposure to prolonged heat"?? Is that an hour? Three hours?? Sitting in the paraffin waiting to be drained.? I would appreciate some insight on this. Second question:? Endom, POC tissue, even some sinus contents arrive wrapped in lens paper.? These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper.? Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 11 Date: Mon, 5 Oct 2009 12:11:54 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] time in paraffin and fried bloody specimen To: Nancy Schmitt Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Nancy, Tissue should be processed @ between 60-62 degrees centigrade. We have three paraffin baths. The 1st bath is set for 45 mins, the 2cd and 3rd are for 1 hour each. This is for large specimens. Small specimens are for 30 mins. the first two baths and 45 mins for the last. It is very true that too much time in paraffin causes hard tissue. Remember the whole time the tissue is setting in paraffin it is being exposed to heat. Your second question: Have the specimen transferred from the lens paper it arrived in and put on a new piece which has been moistened with formalin. Sometimes in surgery the lens paper is wet with saline. If it is a scant amount process with your Endoscopic biopsies. Too long in your alcohols will over dehydrate the specimen. Hope this helps. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Nancy Schmitt Sent by: histonet-bounces@lists.utsouthwestern.edu 10/05/2009 11:07 AM To "Histonet (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] time in paraffin and fried bloody specimen Good Morning Histonetters- First question: Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 71, Issue 5 *************************************** Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From sandra.moeller78 <@t> googlemail.com Mon Oct 5 15:37:32 2009 From: sandra.moeller78 <@t> googlemail.com (Sandra Moeller) Date: Mon Oct 5 15:37:34 2009 Subject: [Histonet] Paraffin sectioning mouse embryos Message-ID: Dear Histonetters, I hope you can help me. I'm working with mouse embryos. Now I have some samples (~ E.10.5), which have already gone through ISH. I tried to make some paraffin sectionings from them, but most of the sectionings were compressed anddid't look very nice. I tried the processing with different times and the slices in different thicknesses. Younger embryos w/o ISH get very hard and break easily. Hopefully one of you has an idea ;-) Thank you very much, Sandra From jm.lapointe <@t> accellab.com Tue Oct 6 09:08:03 2009 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Tue Oct 6 09:08:07 2009 Subject: [Histonet] frozen myocardium sections Message-ID: Hi all, for a study we are freezing myocardium sections in OCT immersed directly in liquid nitrogen, without isopentane, because apparently isopentane quenches the fluorescence of the cells we need to detect in the tissue. Unfortunately the blocks tend to crack after freezing. Does anyone have a suggestion to avoid cracking ? thanks __________________________________ Jean-Martin Lapointe AccelLAB Inc jm.lapointe@accellab.com From mcauliff <@t> umdnj.edu Tue Oct 6 09:32:25 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 6 09:30:38 2009 Subject: [Histonet] frozen myocardium sections In-Reply-To: References: Message-ID: <4ACB54F9.2010902@umdnj.edu> 1. Cool the isopentane with liquid N. Put a metal rod (aluminum, brass, copper) in the chilled isopentane. Wait for the rod to cool. 2. Put the tissue+OCT on a metal object disk, microtome chuck or even a small metal plate. 3. Put the metal supporting the tissue on the chilled metal rod, the tissue will freeze rapidly without cracking or touching the isopentane. Voila! Put the tissue in the cryostat at the appropriate temperature for sectioning and have some coffee while the tissue "warms up" to cutting temperature. Geoff Jean-Martin Lapointe wrote: > Hi all, > > for a study we are freezing myocardium sections in OCT immersed directly > in liquid nitrogen, without isopentane, because apparently isopentane > quenches the fluorescence of the cells we need to detect in the tissue. > Unfortunately the blocks tend to crack after freezing. Does anyone have > a suggestion to avoid cracking ? > > thanks > > > > __________________________________ > > Jean-Martin Lapointe > > AccelLAB Inc > > jm.lapointe@accellab.com > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From abright <@t> brightinstruments.com Tue Oct 6 09:31:41 2009 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Oct 6 09:33:58 2009 Subject: [Histonet] frozen myocardium sections In-Reply-To: References: Message-ID: <314427436-1254839491-cardhu_decombobulator_blackberry.rim.net-2022108336-@bda177.bisx.produk.on.blackberry> Perhaps try freezing @ a higher temperature e.g C02. Regards Alan Bright Bright Instrument Co. Cambridgeshire UK Sent from my BlackBerry? wireless device -----Original Message----- From: "Jean-Martin Lapointe" Date: Tue, 6 Oct 2009 10:08:03 To: Subject: [Histonet] frozen myocardium sections Hi all, for a study we are freezing myocardium sections in OCT immersed directly in liquid nitrogen, without isopentane, because apparently isopentane quenches the fluorescence of the cells we need to detect in the tissue. Unfortunately the blocks tend to crack after freezing. Does anyone have a suggestion to avoid cracking ? thanks __________________________________ Jean-Martin Lapointe AccelLAB Inc jm.lapointe@accellab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Tue Oct 6 09:37:30 2009 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Tue Oct 6 09:37:33 2009 Subject: [Histonet] VEGFR2 antibody Message-ID: <369634.82220.qm@web113101.mail.gq1.yahoo.com> Hi everyone! It's good to be back ... I have resubscribed under a different email as I switched place of employment.? ? I am just running a poll as to what?your?favorite anti-VEGFR2 antibody is for immunostaining human tissue or cells. Frozen or paraffin, cells or tissues ...?it's all good info if you wouldn't mind?sharing what clone/source?works best for you and in what preparation. ? Thanks in advance, Andrea? From es144131 <@t> bcm.tmc.edu Tue Oct 6 10:25:08 2009 From: es144131 <@t> bcm.tmc.edu (Stephens, Elizabeth Humes) Date: Tue Oct 6 10:25:25 2009 Subject: [Histonet] Movat Pentachrome--Saffron stain of collagen Message-ID: I?m trying to find out more information about Movat saffron-straining collagen as it relates to collagen maturation/cross-linking. Does anybody know whether more aligned or more cross-linked collagen will pick up more saffron in a Movat pentachrome stain? (We mostly are dealing with collagen type I in our Movats). I have also been trying to find out more about the mechanism of saffron-staining binding to collagen fibers in the context of the Movat pentachrome stain. If anyone has any information on these topics, it would be much appreciated. Thank you!! Elizabeth From Kimberly.Marshall <@t> ahss.org Tue Oct 6 10:46:02 2009 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Tue Oct 6 10:46:35 2009 Subject: [Histonet] Pneumocystis Controls Message-ID: Hello all Was hoping I could get some info on Pneumocystis Controls. The company I used to get them from has stopped preparing them, and the couple of other companies I have checked with are in short supply, so only selling to customers that buy from them already. Any info would be appreciated. Thanks in advance Kimberly Marshall HT (ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From tp2 <@t> medicine.wisc.edu Tue Oct 6 10:46:20 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Oct 6 10:46:38 2009 Subject: [Histonet] VEGFR2 antibody Message-ID: <4ACB1FFC020000DF0001C7C2@gwmail.medicine.wisc.edu> I was looking at picking one up from Cell Signaling Technology. They also have a phospho specific version. Tom Pier >>> "Andrea T. Hooper" 10/06/09 9:47 AM >>> Hi everyone! It's good to be back ... I have resubscribed under a different email as I switched place of employment. I am just running a poll as to what your favorite anti-VEGFR2 antibody is for immunostaining human tissue or cells. Frozen or paraffin, cells or tissues ... it's all good info if you wouldn't mind sharing what clone/source works best for you and in what preparation. Thanks in advance, Andrea _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shshaw <@t> WPI.EDU Tue Oct 6 10:49:24 2009 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Tue Oct 6 10:48:11 2009 Subject: [Histonet] Rat skin Staining Message-ID: Hello, I'm looking to see if anybody has H&E transverse sections of rat skin that you are willing to send me a copy with the skin sections labeled so I can see what I'm looking at on my sections. Thanks, Sharon-WPI From DKBoyd <@t> chs.net Tue Oct 6 10:54:08 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Oct 6 10:53:54 2009 Subject: [Histonet] Pneumocystis Controls In-Reply-To: Message-ID: Newcomer Supply. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "Marshall, Kimberly" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/06/2009 11:47 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Pneumocystis Controls Hello all Was hoping I could get some info on Pneumocystis Controls. The company I used to get them from has stopped preparing them, and the couple of other companies I have checked with are in short supply, so only selling to customers that buy from them already. Any info would be appreciated. Thanks in advance Kimberly Marshall HT (ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From histo20 <@t> hotmail.com Tue Oct 6 11:35:48 2009 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Tue Oct 6 11:35:55 2009 Subject: [Histonet] Histology positions Message-ID: I have two full time Histology Technician positions available starting October 21st. We are located at St. Joseph Medical Center in Towson, Md. If anyone is interested, please contact Paula Wilder at 410-337-1741. Thank you so much! _________________________________________________________________ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/171222986/direct/01/ From rsrichmond <@t> gmail.com Tue Oct 6 12:29:42 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Oct 6 12:29:46 2009 Subject: [Histonet] Re: Brain frozen sections Message-ID: You guys tell your pathologists to listen to Dr. Hessler - he taught me how to do these preparations, and the basics of interpreting them, when I did a locum tenens for him at the Medical College of Georgia (in Augusta) 6 years ago. I did a lot of them when I got a full time job at a place that did a lot of neurosurgical pathology, a year later. Bob Richmond Samurai Pathologist Knoxville TN ********************* There is nothing you can do to make brain frozen sections acceptable. Your Pathologists need to learn how to read smears, or just accept being wrong 50% of the time. An educated guess based on the imaging is more accurate than frozen sections on intra-axial primary brain tumors. Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN From pkrichar <@t> gundluth.org Tue Oct 6 14:01:16 2009 From: pkrichar <@t> gundluth.org (pkrichar@gundluth.org) Date: Tue Oct 6 14:01:21 2009 Subject: [Histonet] PowerPath and Citrix In-Reply-To: Message-ID: Is there anyone that has PowerPath setup in a Citrix environment that would have IS staff willing to talk with us? Thanks Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org From jaylundgren <@t> gmail.com Tue Oct 6 14:42:07 2009 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Oct 6 14:42:11 2009 Subject: [Histonet] Temp HTL available Message-ID: Currently available for 2 week to 18 month assignments. I am an AFIP trained HTL with an MS in Biotechnology and 20+ years of experience in all aspects of the Pathology laboratory, including 8+ years of travel. Extensive IHC experience. Sterling references. I am the same technologist that you would be getting from the nation's largest medical staffing agency. The only difference? I can save you thousands on a standard 13 week assignment. I am an individual, NOT an agency. From Herrick.James <@t> mayo.edu Tue Oct 6 14:57:36 2009 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Tue Oct 6 14:57:43 2009 Subject: [Histonet] Question on decalcification of canine femur Message-ID: <7267A64D75F58241B577876D8A885631015A97E0@msgebe41> Hello everyone, Would anyone have any experience with decalcifying canine femurs (approximately ?" dia. X 1" length)? We are currently using a Ted Pella microwave with a 14% EDTA solution and a pH of 7.4. The microwave is on for approximately 7 to 8 hours daily (excluding weekends) and the procedure was started on 9-23-09. As of today, a microCT scan was taken to try and determine the degree of decalcification that had taken place. Unfortunately, it does not show a great deal of decalcification at this point. I would think that after 2 weeks we would have had better results. How long should one expect to have to continue this process for a specimen of this type and size? Is there a better way to determine the degree of decalcification? Any advice would be greatly appreciated. Thank you much. Jim Herrick Senior Research Technologist Department of Orthopedics Bone Histomorphometry Core Lab Phone: (507) 255-5946 fax: (507) 266-9451 Email: herrick.james@mayo.edu ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org From leiker <@t> buffalo.edu Tue Oct 6 15:12:01 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Oct 6 15:12:11 2009 Subject: [Histonet] frozen myocardium sections In-Reply-To: <4ACB54F9.2010902@umdnj.edu> References: <4ACB54F9.2010902@umdnj.edu> Message-ID: <1CA1F48AD2006D92AE3D9325@CDYwxp1931.ad.med.buffalo.edu> Hi Geoff, just curious...could you not just plunge the rod directly into the liquid N to cool it? Regards, Merced --On Tuesday, October 06, 2009 10:32 AM -0400 Geoff McAuliffe wrote: > 1. Cool the isopentane with liquid N. Put a metal rod (aluminum, brass, > copper) in the chilled isopentane. Wait for the rod to cool. > 2. Put the tissue+OCT on a metal object disk, microtome chuck or even a > small metal plate. > 3. Put the metal supporting the tissue on the chilled metal rod, the > tissue will freeze rapidly without cracking or touching the isopentane. > Voila! > Put the tissue in the cryostat at the appropriate temperature for > sectioning and have some coffee while the tissue "warms up" to cutting > temperature. > > Geoff > > > Jean-Martin Lapointe wrote: >> Hi all, >> >> for a study we are freezing myocardium sections in OCT immersed directly >> in liquid nitrogen, without isopentane, because apparently isopentane >> quenches the fluorescence of the cells we need to detect in the tissue. >> Unfortunately the blocks tend to crack after freezing. Does anyone have >> a suggestion to avoid cracking ? >> >> thanks >> >> >> >> __________________________________ >> >> Jean-Martin Lapointe >> >> AccelLAB Inc >> >> jm.lapointe@accellab.com >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From twheelock <@t> mclean.harvard.edu Tue Oct 6 17:44:41 2009 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Oct 6 16:12:39 2009 Subject: [Histonet] Re: Brain frozen sections In-Reply-To: References: Message-ID: <4ACBC859.4030407@mclean.harvard.edu> Hi: There is one thing you might try to make frozen brain sections more than acceptable. You can freeze the tissue in liquid nitrogen vapor (LNV) (not directly into liquid nitrogen), let the tissue equilibrate to cryostat temperature, and then cut the sections. The histology is almost as good as a paraffin section. This is assuming that the technique fits the time frame within which you must arrive at a diagnosis. Also we ourselves do not use frozen sections to screen brains diagnostically. We use paraffin sections from the formalin fixed half of the brain. We send the LNV frozen blocks to investigators who cut sections from the frozen blocks for their research. If you would like the details, you can contact one of the following individuals who do this routinely at our brain bank, to see if this protocol is appropriate for your needs. George Tejada 617-855-2646 gtejada@mclean.org Louis Fernandes 617-855-2636 lfernandes@mclean.harvard.edu Good luck. I hope this information helps. Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 Robert Richmond wrote: > You guys tell your pathologists to listen to Dr. Hessler - he taught > me how to do these preparations, and the basics of interpreting them, > when I did a locum tenens for him at the Medical College of Georgia > (in Augusta) 6 years ago. I did a lot of them when I got a full time > job at a place that did a lot of neurosurgical pathology, a year > later. > > Bob Richmond > Samurai Pathologist > Knoxville TN > ********************* > There is nothing you can do to make brain frozen sections acceptable. > Your Pathologists need to learn how to read smears, or just accept > being wrong 50% of the time. An educated guess based on the imaging is > more accurate than frozen sections on intra-axial primary brain > tumors. > > Richard B Hessler, MD > Chief of Pathology > Erlanger Medical Center > Chattanooga, TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mgdelaware <@t> comcast.net Tue Oct 6 17:26:08 2009 From: mgdelaware <@t> comcast.net (marian powers) Date: Tue Oct 6 17:26:56 2009 Subject: [Histonet] FT Histology Position Message-ID: Private Pathology Lab in Dover, DE is seeking a full time HT (ASCP). All inquiries please contact mpowers@dpspa.com From kjgada <@t> gmail.com Tue Oct 6 20:17:09 2009 From: kjgada <@t> gmail.com (Komal Gada) Date: Tue Oct 6 20:17:14 2009 Subject: [Histonet] Licensing? Message-ID: Hello all, I am an ASCP certified Histotechnician (HT), i also have NY state license.Can anyone suggest the best way to obtain a Florida license? Thanks, I appreciate your replies. From annigyg <@t> gmail.com Wed Oct 7 04:32:04 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Oct 7 04:32:10 2009 Subject: [Histonet] Re: Brain frozen sections In-Reply-To: <4ACBC859.4030407@mclean.harvard.edu> References: <4ACBC859.4030407@mclean.harvard.edu> Message-ID: HA! excellent - I ran a neuropath lab for 8 years and we ALWAYS used smears - I used to make the smears and stain the slide - by the time the Path go to the lab all he had to do was read them!! ...but here it is different - they insist on frozens and my techs know that most of the time there will be frezzing artefact I will pass the message to them and then I will run and hide!! LOL ;D thanks greetings from the desert Anne 2009/10/7 Tim Wheelock > Hi: > > There is one thing you might try to make frozen brain sections more than > acceptable. > You can freeze the tissue in liquid nitrogen vapor (LNV) (not directly into > liquid nitrogen), let the tissue equilibrate to cryostat temperature, and > then cut the sections. > The histology is almost as good as a paraffin section. > > This is assuming that the technique fits the time frame within which you > must arrive at a diagnosis. > Also we ourselves do not use frozen sections to screen brains > diagnostically. We use paraffin sections from the formalin fixed half of the > brain. > We send the LNV frozen blocks to investigators who cut sections from the > frozen blocks for their research. > > If you would like the details, you can contact one of the following > individuals who do this routinely at our brain bank, to see if this > protocol is appropriate for your needs. > > > > George Tejada > 617-855-2646 > gtejada@mclean.org > > Louis Fernandes > 617-855-2636 > lfernandes@mclean.harvard.edu > > > Good luck. > I hope this information helps. > > > > Tim Wheelock > Assistant Director, Neuropathology > Instructor In Neuroanatomy > Harvard Brain Tissue Resource Center > 203 Mailman Research Center > McLean Hospital > Belmont MA 02478 > Phone: 617-855-3592 > Fax: 617-855-3199 > > > > Robert Richmond wrote: > >> You guys tell your pathologists to listen to Dr. Hessler - he taught >> me how to do these preparations, and the basics of interpreting them, >> when I did a locum tenens for him at the Medical College of Georgia >> (in Augusta) 6 years ago. I did a lot of them when I got a full time >> job at a place that did a lot of neurosurgical pathology, a year >> later. >> >> Bob Richmond >> Samurai Pathologist >> Knoxville TN >> ********************* >> There is nothing you can do to make brain frozen sections acceptable. >> Your Pathologists need to learn how to read smears, or just accept >> being wrong 50% of the time. An educated guess based on the imaging is >> more accurate than frozen sections on intra-axial primary brain >> tumors. >> >> Richard B Hessler, MD >> Chief of Pathology >> Erlanger Medical Center >> Chattanooga, TN >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rjbuesa <@t> yahoo.com Wed Oct 7 07:10:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 7 07:10:55 2009 Subject: [Histonet] Licensing? In-Reply-To: Message-ID: <17240.60719.qm@web65711.mail.ac4.yahoo.com> The best thing for you to do is to visit the Florida society for histotechnology website. Google the name. Ren? J. --- On Tue, 10/6/09, Komal Gada wrote: From: Komal Gada Subject: [Histonet] Licensing? To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 6, 2009, 9:17 PM Hello all, I am an ASCP certified Histotechnician (HT), i also have NY state license.Can anyone suggest the best way to obtain a Florida license? Thanks, I appreciate your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jeri <@t> opssearchgroup.com Wed Oct 7 07:40:00 2009 From: jeri <@t> opssearchgroup.com (jeri@opssearchgroup.com) Date: Wed Oct 7 07:40:30 2009 Subject: [Histonet] Path lab manager position Message-ID: I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY From sfeher <@t> CMC-NH.ORG Wed Oct 7 09:08:41 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Oct 7 09:09:08 2009 Subject: [Histonet] Licensing? In-Reply-To: <17240.60719.qm@web65711.mail.ac4.yahoo.com> References: <17240.60719.qm@web65711.mail.ac4.yahoo.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB201D@exchange.cmc-nh.org> Here's the link to the Fla DOH Licensure Offices. http://www.doh.state.fl.us/mqa/med-boards.html Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, October 07, 2009 8:11 AM To: histonet@lists.utsouthwestern.edu; Komal Gada Subject: Re: [Histonet] Licensing? The best thing for you to do is to visit the Florida society for histotechnology website. Google the name. Ren? J. --- On Tue, 10/6/09, Komal Gada wrote: From: Komal Gada Subject: [Histonet] Licensing? To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 6, 2009, 9:17 PM Hello all, I am an ASCP certified Histotechnician (HT), i also have NY state license.Can anyone suggest the best way to obtain a Florida license? Thanks, I appreciate your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Oct 7 09:19:48 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Oct 7 09:18:02 2009 Subject: [Histonet] frozen myocardium sections In-Reply-To: <1CA1F48AD2006D92AE3D9325@CDYwxp1931.ad.med.buffalo.edu> References: <4ACB54F9.2010902@umdnj.edu> <1CA1F48AD2006D92AE3D9325@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <4ACCA384.3080409@umdnj.edu> You could but it would be too cold and might crack the tissue. Isopentane becomes a solid and mitagates, for lack of a better word, the coldness of liquid nitrogen. A good alternative would be to cool the rod with dry ice which is only about -80C or so. Geoff Merced M Leiker wrote: > Hi Geoff, just curious...could you not just plunge the rod directly > into the liquid N to cool it? > > Regards, > Merced > > --On Tuesday, October 06, 2009 10:32 AM -0400 Geoff McAuliffe > wrote: > >> 1. Cool the isopentane with liquid N. Put a metal rod (aluminum, brass, >> copper) in the chilled isopentane. Wait for the rod to cool. >> 2. Put the tissue+OCT on a metal object disk, microtome chuck or even a >> small metal plate. >> 3. Put the metal supporting the tissue on the chilled metal rod, the >> tissue will freeze rapidly without cracking or touching the isopentane. >> Voila! >> Put the tissue in the cryostat at the appropriate temperature for >> sectioning and have some coffee while the tissue "warms up" to cutting >> temperature. >> >> Geoff >> >> >> Jean-Martin Lapointe wrote: >>> Hi all, >>> >>> for a study we are freezing myocardium sections in OCT immersed >>> directly >>> in liquid nitrogen, without isopentane, because apparently isopentane >>> quenches the fluorescence of the cells we need to detect in the tissue. >>> Unfortunately the blocks tend to crack after freezing. Does anyone have >>> a suggestion to avoid cracking ? >>> >>> thanks >>> >>> >>> >>> __________________________________ >>> >>> Jean-Martin Lapointe >>> >>> AccelLAB Inc >>> >>> jm.lapointe@accellab.com >>> >>> >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> >> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583 mcauliff@umdnj.edu >> ********************************************** >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From srishan <@t> mail.holyname.org Wed Oct 7 09:25:36 2009 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Wed Oct 7 09:26:40 2009 Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES Message-ID: Hi All, I know this question has been asked before. What is everyone doing about cutting and preparing slides and blocks for legal purposes as well as requests from other hospitals or patients. Is there any charges and how much are the charges? Thanks in advance for your suggestions. Nirmala Srishan __________________ I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From trathborne <@t> somerset-healthcare.com Wed Oct 7 09:38:14 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Oct 7 09:38:20 2009 Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES In-Reply-To: Message-ID: We do not charge for recuts or send outs. Our Medical Records department charges $1 per page for reports. Charging is something that we have discussed for many years, though. I am also interested in what other institutions doe. It takes a lot of time for packing and record keeping even if sending originals (for legal cases), not to mention the time to recut and stain and the cost to send them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of srishan@mail.holyname.org Sent: Wednesday, October 07, 2009 10:26 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL,MEDICAL PURPOSES Hi All, I know this question has been asked before. What is everyone doing about cutting and preparing slides and blocks for legal purposes as well as requests from other hospitals or patients. Is there any charges and how much are the charges? Thanks in advance for your suggestions. Nirmala Srishan __________________ I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From jcampbell <@t> vdxpathology.com Wed Oct 7 09:40:48 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Wed Oct 7 09:40:53 2009 Subject: [Histonet] CD3 on mouse tissue Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82AC37C@VDXSERVER01.vdxpathology.local> Hi Histonetters, I'm trying to work up CD3 on mouse tissue. I knew I would have to get a mouse-on-mouse kit since CD3 is a rabbit polyclonal but do you use some sort of rodent kit to eliminate background staining? My secondary antibody is part of a multilink kit from Biogenex and is capable of recognizing both mouse and rabbit primary antibodies. So, I'm afraid that it is recognizing endogenous antibodies in my mouse tissue. Any recommendations? Thanks, Jennifer From Wanda.Smith <@t> HCAhealthcare.com Wed Oct 7 09:46:33 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Oct 7 09:46:35 2009 Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1376F00075@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All, I have developed a fee schedule for legal cases and I have a form letter that I send to the offices before any slides are sent. We only furnish 1 H&E and 2 unstained slides per block, if tissue is adequate. We never send original slides and we never send blocks. My charges are: $25.00 for looking up the case and up to 5 copies $12.50 per slide and a minimum of 3 slides per block They pay any shipping other than US mail. Sometimes the lawyer's office only wants reports and sometimes I have received checks for up to $375.00. If a patient or institution request blocks for Continuation of Care, we either send the original slides with a request for them to be returned or we do recuts at no charge to the patient or the requesting institution. Hope this helps!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Wednesday, October 07, 2009 10:26 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES Hi All, I know this question has been asked before. What is everyone doing about cutting and preparing slides and blocks for legal purposes as well as requests from other hospitals or patients. Is there any charges and how much are the charges? Thanks in advance for your suggestions. Nirmala Srishan __________________ I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Wed Oct 7 09:51:32 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Oct 7 09:51:15 2009 Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES In-Reply-To: Message-ID: For legal cases we charge $25.00 per slide and send on their FedEx account. We do not charge for patient referral work, it is considered a courtesy for using our facility. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net srishan@mail.holyname.org Sent by: histonet-bounces@lists.utsouthwestern.edu 10/07/2009 10:27 AM To histonet-bounces@lists.utsouthwestern.edu, cc Subject [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES Hi All, I know this question has been asked before. What is everyone doing about cutting and preparing slides and blocks for legal purposes as well as requests from other hospitals or patients. Is there any charges and how much are the charges? Thanks in advance for your suggestions. Nirmala Srishan __________________ I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From NSEARCY <@t> swmail.sw.org Wed Oct 7 10:38:09 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Oct 7 10:38:17 2009 Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL,MEDICAL PURPOSES In-Reply-To: References: Message-ID: <4ACC6F91.5D38.00EF.0@swmail.sw.org> I have been charging for legal cases for years; other institutions there is no charge---patient carte issue. We have a $60.00 search fee ( transcription & histology expense offset) ; if we recut- $25.00 for each H&E; special stains- $50; immunohistochemistry- $75.00. Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Rathborne, Toni" 10/7/2009 9:38 AM >>> We do not charge for recuts or send outs. Our Medical Records department charges $1 per page for reports. Charging is something that we have discussed for many years, though. I am also interested in what other institutions doe. It takes a lot of time for packing and record keeping even if sending originals (for legal cases), not to mention the time to recut and stain and the cost to send them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of srishan@mail.holyname.org Sent: Wednesday, October 07, 2009 10:26 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL,MEDICAL PURPOSES Hi All, I know this question has been asked before. What is everyone doing about cutting and preparing slides and blocks for legal purposes as well as requests from other hospitals or patients. Is there any charges and how much are the charges? Thanks in advance for your suggestions. Nirmala Srishan __________________ I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From Jessica.Vacca <@t> HCAhealthcare.com Wed Oct 7 10:56:57 2009 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Wed Oct 7 10:57:22 2009 Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1376F00075@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1376F00075@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <938D716CD445614ABBB817517557B6F4CD602BCB@NADCWPMSGCMS09.hca.corpad.net> I would like to know how you went about establishing the search fee, there are many times that these legal companies like Litigation management, Record trak, central records depository that just do a mass letter requesting records, and there is no pathology to be found, then they ask for you to sign and motorize their documents. I would absolutely love to tell them that there is a fee to search. Who established the fee your legal department or the lab? We charge 50 for the case, 10 per block after the first block, and 25 for additional cases with the 10 fee still applying for each additional block. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 07, 2009 10:47 AM To: srishan@mail.holyname.org; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES Good Morning to All, I have developed a fee schedule for legal cases and I have a form letter that I send to the offices before any slides are sent. We only furnish 1 H&E and 2 unstained slides per block, if tissue is adequate. We never send original slides and we never send blocks. My charges are: $25.00 for looking up the case and up to 5 copies $12.50 per slide and a minimum of 3 slides per block They pay any shipping other than US mail. Sometimes the lawyer's office only wants reports and sometimes I have received checks for up to $375.00. If a patient or institution request blocks for Continuation of Care, we either send the original slides with a request for them to be returned or we do recuts at no charge to the patient or the requesting institution. Hope this helps!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Wednesday, October 07, 2009 10:26 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES Hi All, I know this question has been asked before. What is everyone doing about cutting and preparing slides and blocks for legal purposes as well as requests from other hospitals or patients. Is there any charges and how much are the charges? Thanks in advance for your suggestions. Nirmala Srishan __________________ I am seeking a Pathology Manager for a hospital system located in a great city in the Pacific NW. The manager would oversee three supervisors who manage Histo, Cyto and Pathology Transcription. There are about 60 Full Time employees working under these Supervisors. The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). For more details contact me at 574.633.1231. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brod033 <@t> gmail.com Wed Oct 7 12:33:50 2009 From: brod033 <@t> gmail.com (Ben Spirto) Date: Wed Oct 7 12:33:54 2009 Subject: [Histonet] Histology positions In-Reply-To: References: Message-ID: <287d127c0910071033l75674f5neac6260ca80f66c1@mail.gmail.com> Hi; I am looking for part-time (nights and weekends) position as a HTL or HT or any lab position in Chicagoland area. Experience in immunohistochemistry. Please reply to: brod033@gmail.com Thank you On Tue, Oct 6, 2009 at 11:35 AM, Paula Wilder wrote: > > I have two full time Histology Technician positions available starting > October 21st. We are located at St. Joseph Medical Center in Towson, Md. > If anyone is interested, please contact Paula Wilder at 410-337-1741. > Thank you so much! > _________________________________________________________________ > Hotmail: Powerful Free email with security by Microsoft. > > http://clk.atdmt.com/GBL/go/171222986/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Annette.Fletcher <@t> providence.org Wed Oct 7 13:01:07 2009 From: Annette.Fletcher <@t> providence.org (Fletcher, Annette M) Date: Wed Oct 7 13:01:33 2009 Subject: [Histonet] Pathology Manager position with Providence in Portland, Oregon Message-ID: <284596E350CB0743BB500B18475E7A0B02F44E7B@wn1223.or.providence.org> Dear Histonetters, (no recruiters please) Providence Health and Services is seeking a Pathology Manager in Portland, OR to oversee three supervisors who manage Histo, Cyto and Pathology Transcription. This is a new position! There are about 60 Full Time employees working under these Supervisors. The hiring range for this will be somewhere between 95K and 100K (possible room for negotiation). Here are the job description and requirements: General Summary: The position of Pathology Manager is one primarily focused on delivering pathology related service commitments to six hospitals and numerous outreach customers within the State of Oregon by coordinating the technical and support sections of cytopathology processing and screening, histology, surgical pathology grossing and transcription with the professional physician staff (pathologists). Position requires a Bachelors degree in healthcare or related field with additional formal training in finance, economics, purchasing and operations management. A minimum of 10 year's progressive medical/healthcare management experience, in a high volume facility, preferably with bottom line accountability in a for profit environment. Familiarity with all aspects of department operations, including cost effective management of labor and supplies. Familiarity with workflow and operational processes between technical and non-technical functions and physician staff. Demonstrated ability to achieve operational improvement leading to margin enhancement. Ability to identify root causes for operational and financial variances and to remediate. Preferred: MBA. Please call me directly at 503-215-5840 or email me with any questions. Kind Regards, Annette M Fletcher Senior Recruiter Providence Health & Services, Employment 1235 NE 47th Avenue - Suite 200 Portland, OR 97213 T: 503-215-5840 F: 503-215-5770 Annette.fletcher@providence.org Kind Regards, Annette M Fletcher Senior Recruiter Providence Health & Services, Employment 1235 NE 47th Avenue - Suite 200 Portland, OR 97213 T: 503-215-5840 F: 503-215-5770 Annette.fletcher@providence.org ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From jcarpenter764 <@t> aol.com Wed Oct 7 14:22:42 2009 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Wed Oct 7 14:23:00 2009 Subject: [Histonet] regulations or procedures for "pre-labeling slides"??? Message-ID: <8CC159AAD1CF9F2-25FC-28BAC@webmail-d001.sysops.aol.com> Does anyone know where I can find the protocal or know the protocal for prelabeling slides before cutting the tissue? From Margaret.Perry <@t> sdstate.edu Wed Oct 7 14:24:57 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Oct 7 14:26:15 2009 Subject: [Histonet] tris buffer Message-ID: I am trying a protocol that uses 0.1M Tris/0.15M sodium chloride pH7.5, which is what I normally use. At another step in the protocol it says to wash with 0.05M Tris buffer pH 7.5. What do I use to make the 0.05M Tris buffer? Is it a combination of Tris HCl and Tris base without sodium chloride? From enrriq88 <@t> yahoo.com Wed Oct 7 15:05:03 2009 From: enrriq88 <@t> yahoo.com (Jaime Plata) Date: Wed Oct 7 15:05:08 2009 Subject: [Histonet] Licensing? In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190FDB201D@exchange.cmc-nh.org> Message-ID: <25469.41702.qm@web50409.mail.re2.yahoo.com> Contact State of Department of Health Division of Medical Quality Assurance. Be careful in your selection. I did move from Va down here and the housing (realstate) and pay hourly it is no as good we thought. Jaime E Plata BS. MT. HTL (ASCP) Clinical Lab Supervisor Supervisor State of Florida Department of Health --- On Wed, 10/7/09, Feher, Stephen wrote: From: Feher, Stephen Subject: RE: [Histonet] Licensing? To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, "Komal Gada" Date: Wednesday, October 7, 2009, 10:08 AM Here's the link to the Fla DOH Licensure Offices. http://www.doh.state.fl.us/mqa/med-boards.html Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, October 07, 2009 8:11 AM To: histonet@lists.utsouthwestern.edu; Komal Gada Subject: Re: [Histonet] Licensing? The best thing for you to do is to visit the Florida society for histotechnology website. Google the name. Ren? J. --- On Tue, 10/6/09, Komal Gada wrote: From: Komal Gada Subject: [Histonet] Licensing? To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 6, 2009, 9:17 PM Hello all, I am an ASCP certified Histotechnician (HT), i also have NY state license.Can anyone suggest the best way to obtain a Florida license? Thanks, I appreciate your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Wed Oct 7 15:56:49 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Oct 7 15:57:05 2009 Subject: [Histonet] Sudan Black stain for fluid smear Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDBC9EE2@SJSNT-SCMAIL03.stjoe.org> Anyone know of a Sudan Black technique for peritoneal fluid smear ??? Or if it's even possible Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Albert.Santiago <@t> uphs.upenn.edu Wed Oct 7 17:35:29 2009 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Oct 7 17:35:35 2009 Subject: [Histonet] (no subject) Message-ID: Hello fellow histonetters, I was hoping someone can tell me how long it is required to store patient serum for indirect immunofluorescence and patient tissue frozen in OCT medium for direct immunofluorescence. Also, how long do we store the slides produced by these two procedures? Also, the source where I can refer to. Thank you very much for your help. My email address is, albert.santiago@uphs.upenn.edu Albert Santiago, HT(ASCP) Lab Supervisor Dermatopathology 215-662-6539-office 215-662-6150-fax The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From macveigh <@t> usc.edu Wed Oct 7 17:50:24 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Oct 7 17:50:16 2009 Subject: [Histonet] Re: Frozen Myocardium sections Message-ID: <003501ca47a0$8ed05d80$5c237d80@DFS66DD1> The OCT cracks if the block remains in liquid N too long. I use a plastic mold, which holds my tissue in the OCT. Float the mold on the surface of the liquid N but pull it out of there while there is still a little liquid/clear OCT (about 6-7mm in diameter) in the middle of the forming block. Put it on the counter (room temp.). As the block sits on the counter, for a minute or two, the freezing of the center takes place. The block will never crack if it is pulled out of the OCT on time. Michelle Aloni MS HTL ASCP USC Keck School of Medicine Los Angeles, CA From macveigh <@t> usc.edu Wed Oct 7 19:11:47 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Oct 7 19:11:35 2009 Subject: [Histonet] Licensing for Massachusetts? Message-ID: <011501ca47ab$ed21b770$5c237d80@DFS66DD1> Hi all, I live in Los Angeles and have HT and HTL from CA. I would like to move to Cape Cod in couple of years. I know that one needs a special Florida license to work in Fl, but do I need a special license from Massachusetts to be able to do histology there? I would appreciate any info Michelle Aloni USC Keck School of Medicine Los Angeles, CA From rmoody <@t> ameripath.com Wed Oct 7 23:08:42 2009 From: rmoody <@t> ameripath.com (Moody, Robert) Date: Wed Oct 7 23:08:51 2009 Subject: [Histonet] (no subject) Message-ID: Hi all can some one please give me the web site to apply for newyork state license From anand <@t> ethoneuro.com Thu Oct 8 01:46:36 2009 From: anand <@t> ethoneuro.com (Anand Vasudevan) Date: Thu Oct 8 01:47:01 2009 Subject: [Histonet] frozen sections for in situ hybridization Message-ID: <189449020910072346i110d0756y3159fcdf22ff50c1@mail.gmail.com> Hi, I have obtained fresh rat brains (not fixed) that have been directly snap-freezed in isopentane and stored at -80C. I plan to section 20um thick. I want to know if there is way to store the cut sections before I carry out the in situ hybridization without thawing and freezing? taht is, the cut sections will be frozen but when placed on slides, but they will thaw before I can store them in the fridge (either -20C or -80C) and then before conducting my experimemt i will have to thaw it again. Thanks, Anand Vasudevan Nanyang Technological University Singapore From melanie.Black <@t> uct.ac.za Thu Oct 8 02:08:20 2009 From: melanie.Black <@t> uct.ac.za (Melanie Black) Date: Thu Oct 8 02:09:45 2009 Subject: [Histonet] Embedding and Sectioning of tissue containing Gels. Message-ID: Hi All We are looking at rat issue which has had a gel subcutaneously injected into it. We have routinely processed it and embedded it into was. The gel appears to survive processing and embedding, but then seems to be pulled out or distorted during sectioning. We would like to visualize it in tact to look at cells within the gel. I have alternately embedded it in JB 4 resin (which is water soluble and does not require dehydration. This method is so far the best, however there are still some challenges. i) There are sometimes bubbles in the resin that occur during polymerization. ii) Some gels, depending on formulations don't take up the resin entirely, and therefore are still too soft to section. We use a glass knife to section this resin. Staining is good, and immunofluorescence in the form of ED-1 and Actin are successful. Does anybody have any experience with this type of research related problem? Many Thanks Melanie Black Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From Melanie.Black <@t> uct.ac.za Thu Oct 8 03:41:03 2009 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Thu Oct 8 03:42:34 2009 Subject: [Histonet] Embedding and Sectioning of tissue containing Gels. References: Message-ID: <4D221012-03E8-4763-9428-4CB78082DC94@uct.ac.za> corrected a spelling error! > > Hi All > > We are looking at rat issue which has had a gel subcutaneously > injected into it. We have routinely processed it and embedded it > into wax. The gel appears to survive processing and embedding, but > then seems to be pulled out or distorted during sectioning. We > would like to visualize it in tact to look at cells within the gel. > > I have alternately embedded it in JB 4 resin (which is water > soluble and does not require dehydration. This method is so far the > best, however there are still some challenges. > i) There are sometimes bubbles in the resin that occur during > polymerization. > ii) Some gels, depending on formulations don't take up the resin > entirely, and therefore are still too soft to section. > > We use a glass knife to section this resin. Staining is good, and > immunofluorescence in the form of ED-1 and Actin are successful. > > Does anybody have any experience with this type of research related > problem? > > Many Thanks > Melanie Black > > Melanie Black > 082 469 3352 > > Cardiovascular Research Unit > 3rd Floor; Chris Barnard Building > Medical School; > Observatory. 7925. > University of Cape Town. > South Africa. > > > Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From rjbuesa <@t> yahoo.com Thu Oct 8 08:06:29 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 8 08:06:33 2009 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <429164.17895.qm@web65701.mail.ac4.yahoo.com> This is what I used to do: take photomocrographs of the results and once done that I disposed of the serum (indirect IF) and the piece of tissue (DIF). That is how I set my SOP and never had a problem with that. Ren? J. ? --- On Wed, 10/7/09, Santiago, Albert wrote: From: Santiago, Albert Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 7, 2009, 6:35 PM Hello fellow histonetters, I was hoping someone can tell me how long it is required to store patient serum for indirect immunofluorescence and patient tissue frozen in OCT medium for direct immunofluorescence. Also, how long do we store the slides produced by these two procedures? Also, the source where I can refer to. Thank you very much for your help. My email address is,? albert.santiago@uphs.upenn.edu Albert Santiago, HT(ASCP) Lab Supervisor Dermatopathology 215-662-6539-office 215-662-6150-fax The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 8 08:14:18 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 8 08:14:28 2009 Subject: [Histonet] Licensing for Massachusetts? In-Reply-To: <011501ca47ab$ed21b770$5c237d80@DFS66DD1> Message-ID: <422075.3161.qm@web65716.mail.ac4.yahoo.com> If nothing has changed, only 12 union states and the associated state of?Puerto Rico?require a license to practice histology: California, Florida, Georgia, Hawaii, Louisiana, Montana, Nevada, New York, North Dakota, Rhode Island, Tennessee and?West Virginia. Massachusetts is not amongst them. Ren? J. --- On Wed, 10/7/09, Michelle MacVeigh-Aloni wrote: From: Michelle MacVeigh-Aloni Subject: [Histonet] Licensing for Massachusetts? To: Histonet@lists.utsouthwestern.edu Date: Wednesday, October 7, 2009, 8:11 PM Hi all, I live in Los Angeles and have HT and HTL from CA. I would like to move to Cape Cod in couple of years. I know that one needs a special Florida license to work in Fl, but do I need a special license from Massachusetts to be able to do histology there? I would appreciate any info Michelle Aloni USC Keck School of Medicine Los Angeles, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From sbreeden <@t> nmda.nmsu.edu Thu Oct 8 08:37:22 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Oct 8 08:37:29 2009 Subject: [Histonet] Price per slide Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46B63@nmdamailsvr.nmda.ad.nmsu.edu> My Daily Challenge is to figure out how much it costs me to produce one slide. I hate reinventing the wheel and I know someone has already either created a formula for doing this, or has a figure already computed for a veterinary lab. I know there would be some variables (salary, cost of water, phase of the moon, etc.) but how did you do it? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From leiker <@t> buffalo.edu Thu Oct 8 08:51:09 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Oct 8 08:51:14 2009 Subject: [Histonet] Re: Frozen Myocardium sections In-Reply-To: <003501ca47a0$8ed05d80$5c237d80@DFS66DD1> References: <003501ca47a0$8ed05d80$5c237d80@DFS66DD1> Message-ID: <798C4E43293E70B42FB3E399@CDYwxp1931.ad.med.buffalo.edu> You can also safely freeze the molds (containing OCT and your specimen) over liquid N vapors by suspending them over the surface of the nitrogen (I just remembered we used to do this some years ago in another lab). Not too far from the surface, though; they'll freeze too slowly and you'll get artifacts. Regards, Merced --On Wednesday, October 07, 2009 3:50 PM -0700 Michelle MacVeigh-Aloni wrote: > The OCT cracks if the block remains in liquid N too long. > > I use a plastic mold, which holds my tissue in the OCT. > Float the mold on the surface of the liquid N but pull it out of there > while there is still a little liquid/clear OCT (about 6-7mm in diameter) > in the middle of the forming block. Put it on the counter (room temp.). > As the block sits on the counter, for a minute or two, the freezing of > the center takes place. The block will never crack if it is pulled out of > the OCT on time. > > Michelle Aloni MS HTL ASCP > USC Keck School of Medicine > Los Angeles, CA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Arlene.Armandi <@t> cshs.org Thu Oct 8 08:56:42 2009 From: Arlene.Armandi <@t> cshs.org (Armandi, Arlene) Date: Thu Oct 8 08:57:47 2009 Subject: [Histonet] Licensing for Massachusetts? In-Reply-To: <422075.3161.qm@web65716.mail.ac4.yahoo.com> Message-ID: There is no state license for Histology in California Arlene Armandi, HTL, HT(ASCP) Cedars-Sinai Medical Center Los Angeles, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 08, 2009 6:14 AM To: Histonet@lists.utsouthwestern.edu; Michelle MacVeigh-Aloni Subject: Re: [Histonet] Licensing for Massachusetts? If nothing has changed, only 12 union states and the associated state of?Puerto Rico?require a license to practice histology: California, Florida, Georgia, Hawaii, Louisiana, Montana, Nevada, New York, North Dakota, Rhode Island, Tennessee and?West Virginia. Massachusetts is not amongst them. Ren? J. --- On Wed, 10/7/09, Michelle MacVeigh-Aloni wrote: From: Michelle MacVeigh-Aloni Subject: [Histonet] Licensing for Massachusetts? To: Histonet@lists.utsouthwestern.edu Date: Wednesday, October 7, 2009, 8:11 PM Hi all, I live in Los Angeles and have HT and HTL from CA. I would like to move to Cape Cod in couple of years. I know that one needs a special Florida license to work in Fl, but do I need a special license from Massachusetts to be able to do histology there? I would appreciate any info Michelle Aloni USC Keck School of Medicine Los Angeles, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From lpaveli1 <@t> hurleymc.com Thu Oct 8 09:12:57 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Oct 8 09:13:11 2009 Subject: [Histonet] Price per slide In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46B63@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46B63@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4ACDBB29.59CD.00EE.0@hurleymc.com> There is an article written by Rene' Buesa in "Advance" for Medical Laboratory Professionals, Jan 15, 2007. You will find everything you need in that article. Very helpful. Lynette >>> "Breeden, Sara" 10/8/2009 9:37 AM >>> My Daily Challenge is to figure out how much it costs me to produce one slide. I hate reinventing the wheel and I know someone has already either created a formula for doing this, or has a figure already computed for a veterinary lab. I know there would be some variables (salary, cost of water, phase of the moon, etc.) but how did you do it? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Thu Oct 8 10:04:33 2009 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Oct 8 10:04:37 2009 Subject: [Histonet] Whole Prostate Processing Message-ID: <1872B4A455B7974391609AD8034C79FC8BD61C@LBEXCH01.hchd.local> Hi to all in histoland. For those of you doing whole prostate processing, could you share the process with me. Our lab is thinking about doing this. I would need a list of equipment that is required as well as processing times. At the present time I only have 2 processors, and they both run every night. I know that I wll need 1 dedicated one for this. Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From jstaruk <@t> masshistology.com Thu Oct 8 10:24:58 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Thu Oct 8 10:24:46 2009 Subject: [Histonet] Price per slide In-Reply-To: <4ACDBB29.59CD.00EE.0@hurleymc.com> Message-ID: <829006948255463C9B2F814F4AC84168@JimPC> I have read this article titled "Histo Procedures: Examining Costs" and must say that this article is quite naive and I doubt very much that the author signs all of the checks associated with producing one glass slide. One example is salary. The author uses $15 to $25 per hour for what it costs for labor. What about the matching Social Security taxes, worker's Comp. insurance, all of the various federal and state employee taxes and fees, matching 401K, holidays, vacations, sick days I have to pay for? My $20 per hour employee actually costs me close to $40 per hour. I didn't thoroughly memorize this article, but I don't recall utilities (HVAC, electricity, water and telephones) being added into the equation. Then there's rent, liability insurance, hazardous waste removal, chemicals and stains, dumpster fees, the secretary that logs in the specimens, the grosser who processes the specimen and the lab aide that labels the slides. There's probably a hundred other peripheral payments I make just to produce that one microscope slide. Finally, in the conclusion portion of the article, the author states that is costs between $4.71 to $5.31 to gross in the specimen and prepare a microscope slide. If that were true, I'd be a very rich man! Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, October 08, 2009 10:13 AM To: histonet@lists.utsouthwestern.edu; Sara Breeden Subject: Re: [Histonet] Price per slide There is an article written by Rene' Buesa in "Advance" for Medical Laboratory Professionals, Jan 15, 2007. You will find everything you need in that article. Very helpful. Lynette >>> "Breeden, Sara" 10/8/2009 9:37 AM >>> My Daily Challenge is to figure out how much it costs me to produce one slide. I hate reinventing the wheel and I know someone has already either created a formula for doing this, or has a figure already computed for a veterinary lab. I know there would be some variables (salary, cost of water, phase of the moon, etc.) but how did you do it? Thanks. From contact <@t> excaliburpathology.com Thu Oct 8 10:38:01 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Oct 8 10:38:04 2009 Subject: [Histonet] Price per slide In-Reply-To: <829006948255463C9B2F814F4AC84168@JimPC> References: <829006948255463C9B2F814F4AC84168@JimPC> Message-ID: <492896.94129.qm@web1116.biz.mail.sk1.yahoo.com> Amen! &?internet costs for sending and receiving email & photomicrographs,?and researching articles! ________________________________ From: jstaruk To: histonet@lists.utsouthwestern.edu; Sara Breeden Sent: Thursday, October 8, 2009 10:24:58 AM Subject: RE: [Histonet] Price per slide I have read this article titled "Histo Procedures: Examining Costs" and must say that this article is quite naive and I doubt very much that the author signs all of the checks associated with producing one glass slide.? One example is salary.? The author uses $15 to $25 per hour for what it costs for labor.? What about the matching Social Security taxes, worker's Comp. insurance, all of the various federal and state employee taxes and fees, matching 401K, holidays, vacations, sick days I have to pay for?? My $20 per hour employee actually costs me close to $40 per hour.? I didn't thoroughly memorize this article, but I don't recall utilities (HVAC, electricity, water and telephones) being added into the equation.? Then there's rent, liability insurance, hazardous waste removal, chemicals and stains, dumpster fees, the secretary that logs in the specimens, the grosser who processes the specimen and the lab aide that labels the slides.? There's probably a hundred other peripheral payments I make just to produce that one microscope slide.? Finally, in the conclusion portion of the article, the author states that is costs between $4.71 to $5.31 to gross in the specimen and prepare a microscope slide.? If that were true, I'd be a very rich man! Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com ? www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, October 08, 2009 10:13 AM To: histonet@lists.utsouthwestern.edu; Sara Breeden Subject: Re: [Histonet] Price per slide There is an article written by Rene' Buesa in "Advance" for Medical Laboratory Professionals, Jan 15, 2007.? You will find everything you need in that article.? Very helpful. Lynette >>> "Breeden, Sara" 10/8/2009 9:37 AM >>> My Daily Challenge is to figure out how much it costs me to produce one slide.? I hate reinventing the wheel and I know someone has already either created a formula for doing this, or has a figure already computed for a veterinary lab.? I know there would be some variables (salary, cost of water, phase of the moon, etc.) but how did you do it? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Thu Oct 8 10:43:12 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Oct 8 10:43:28 2009 Subject: [Histonet] Price per slide In-Reply-To: <492896.94129.qm@web1116.biz.mail.sk1.yahoo.com> References: <829006948255463C9B2F814F4AC84168@JimPC> <492896.94129.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: <4ACDD050.59CD.00EE.0@hurleymc.com> I found the cost article in my file from Advance. If you would like to take a look at it to help in your decision making, I can fax it to you. Is it Friday yet??!!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 From Jackie.O'Connor <@t> abbott.com Thu Oct 8 10:45:24 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Oct 8 10:46:30 2009 Subject: [Histonet] LCA antibody for FFPE mouse In-Reply-To: <492896.94129.qm@web1116.biz.mail.sk1.yahoo.com> References: <829006948255463C9B2F814F4AC84168@JimPC> <492896.94129.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: Does anyone know of an antibody for CD45 that will work in FFPE murine tissue? Thanks. From ncollins <@t> system1.net Thu Oct 8 10:57:39 2009 From: ncollins <@t> system1.net (Noelle Collins) Date: Thu Oct 8 10:53:45 2009 Subject: [Histonet] Pathology Jobs Message-ID: Hello everyone! My name is Noelle, and I am an Executive Search Consultant for System 1 Search, in Greenville, South Carolina. System 1 is a 35 year old recruiting firm that specializes in the permanent placement of pathologists of all subspecialties. I am currently working on opportunities for Dermatopathologists, Surgical Pathologists, Cytopathologists, Hematopathologists, GI or GU Pathologists, and a Medical Director position as well. If anyone knows of a Pathologist who is looking for an opportunity, I would appreciate it if you would pass along my contact information and have them contact me at their earliest convenience. Thank you for any help you can provide! I hope you have a great weekend! Noelle Collins Executive Recruiter System 1 Search 864-528-5065 ncollins@system1.net http://www.system1.net/Pathology.htm Please join me at LinkedIn: http://www.linkedin.com/in/noellehcollins From thomas.crowell <@t> novartis.com Thu Oct 8 12:10:46 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Oct 8 12:10:51 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 10/08/2009 and will not return until 10/09/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. From NMargaryan <@t> childrensmemorial.org Thu Oct 8 12:15:50 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Oct 8 12:17:45 2009 Subject: [Histonet] black DAB instead of brown Message-ID: Dear Histometters: I am getting black staining with my DAB instead of brown. I use usual protocol with AR-pH6, H2O2, Avidin/Biotin, PB, 1?, biotinylated 2-dary, streptavidin then DAB. Does any of use know this kind of problem? and What exactly can coast this artifact? Thanks in advance, Naira From contact <@t> excaliburpathology.com Thu Oct 8 12:23:27 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Oct 8 12:23:31 2009 Subject: [Histonet] black DAB instead of brown In-Reply-To: References: Message-ID: <788461.47102.qm@web1105.biz.mail.sk1.yahoo.com> The cause for DAB to turn black would be the introduction of a metal such as nickel or iron. ________________________________ From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, October 8, 2009 12:15:50 PM Subject: [Histonet] black DAB instead of brown Dear Histometters: I am getting black staining with my DAB instead of brown. I use usual protocol with AR-pH6, H2O2, Avidin/Biotin, PB, 1?, biotinylated 2-dary, streptavidin then DAB. Does any of use know this kind of problem? and What exactly can coast this artifact? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dawn_cowie <@t> yahoo.com Thu Oct 8 15:55:51 2009 From: dawn_cowie <@t> yahoo.com (Dawn Cowie) Date: Thu Oct 8 15:55:56 2009 Subject: [Histonet] (no subject) Message-ID: <222181.66571.qm@web45005.mail.sp1.yahoo.com> To the Listserver Host, Please unsubscribe me from the histonet. ? Thank you, Dawn L. Cowie From W.E.J.Hoekert <@t> olvg.nl Fri Oct 9 06:19:28 2009 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Oct 9 06:27:22 2009 Subject: [Histonet] Labvision immunostainers skip slides Message-ID: <1190CB05C44B13409483514729C2FC360C0A36@PAIT42.olvg.nl> Dear Histonetters, A few months ago, we bought 2 Labvision Autostainers 720 from Thermo (immunostainers). We have some bad experiences with them. They seem to skip some slides every now and than (it happens maybe once every 3-4 weeks, we stain about 150 - 200 slides a day). If so, our positive controls are virtually completely negative, and the patient slides are negative as well. If this happens, we see empty 'holes' in the nuclei. Click here to see an image: http://www.immunoportal.com/modules.php?name=gallery2&g2_itemId=14523&g2_imageViewsIndex=1 All the other antibodies from the same run work fine, and if we repeat the failed cases (without changing anything) they turn out fine. Does anybody recognize this problem or knows what is going on? We dewax in PT modules (the problem occurs both with citraclere and with PT Module Buffer 1 from immunologic). I hope to hear from you. Willem Hoekert Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From cbobrowi <@t> mcw.edu Fri Oct 9 07:46:06 2009 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Fri Oct 9 07:48:06 2009 Subject: [Histonet] Unsubscribe Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AFBE@guyton.phys.mcw.edu> Unsubscribe Carol From Michel.Bataillon <@t> citox.com Fri Oct 9 08:32:18 2009 From: Michel.Bataillon <@t> citox.com (Bataillon Michel) Date: Fri Oct 9 08:32:26 2009 Subject: [Histonet] Mager protocol for zinc Message-ID: <1FB9362B907EEC43959F72445AC63B6339C397@apollon.cit_serveur.com> Does anyone know the Mager protocol for zinc? Thank you. Michel BATAILLON Lab Manager CIT Evreux, France ************************************************************* Ce message et toutes les pieces jointes sont confidentiels et etablis a l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le detruire et d'en avertir immediatement l'expediteur. Toute utilisation de ce message non conforme a sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'integrite de ce message, le CIT decline toute responsabilite au titre de ce message s'il a ete altere, deforme ou falsifie. Protegeons ensemble l'environnement, n'imprimons ce mail (et ses pieces jointes) que si cela est necessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. ************************************************************* From brett_connolly <@t> merck.com Fri Oct 9 08:43:12 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Oct 9 08:43:17 2009 Subject: [Histonet] Automated H&E stainers Message-ID: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> Which one do you like?...pro&cons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From alfredpe <@t> mail.med.upenn.edu Fri Oct 9 08:51:15 2009 From: alfredpe <@t> mail.med.upenn.edu (Alfred Penzo Mendez) Date: Fri Oct 9 08:51:19 2009 Subject: [Histonet] manual processing help Message-ID: <990466142.1549351255096275255.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Dear Histoners, I used to process mouse tissues using a vacuum oven and never ran into any problems. I recently moved to a new lab and there is no vacuum oves, but I've been told that vacuum is not an absolute requirement for paraffin infiltration, and I only need to double the paraffin infiltration changes; however I've tried a coule of times and I can't get anything embedded. Liver and pancreas become vey hard and turn to dust when I try to section them. Gut cuts, but sections have lots of compressions and wrinkles. Does anyone have an idea of why is this happening? here is the full procedure: -Tissues: adult mouse liver, pancreas and gut cut into small pieces (1mm3) -Fix: zinc-formalin (polysciences) 1 h Room temp + o/n at 4oC. Tissues is fixed in 10 ml. -Dehydration: 1 hour each 50%, 70%, 95%, 1005 ethanol + o/n in ethanol at 4oC. -Clearing: 2x 30 minutes in xylene (also tried 2x 1 hour, same result, room temp -Paraffin: 3x 1 hour in paraplast at 60oC Many thanks, Alfredo. From jely <@t> mdanderson.org Fri Oct 9 09:04:14 2009 From: jely <@t> mdanderson.org (Ely,Jeanenne C) Date: Fri Oct 9 09:06:40 2009 Subject: [Histonet] Cytokeratin IHC in Mouse Thymus Message-ID: Hello Histonet: Has anyone out there used a cytokeratin antibody to detect epithelial cells in the thymus of a mouse (FFPE)? If so, which antibody/company? We have tried the monoclonal AE1/AE3 from Dako with mixed results. Thanks so much for any info... Jeanenne Ely, BS, HT(ASCP), QIHC Chief Histology Laboratory MD Anderson Cancer Center Veterinary Medicine & Surgery Unit 63 1515 Holcombe Blvd, Houston, TX 77030 (713) 792-2793 From rjbuesa <@t> yahoo.com Fri Oct 9 09:11:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 9 09:11:53 2009 Subject: [Histonet] Automated H&E stainers In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> Message-ID: <347591.96150.qm@web65704.mail.ac4.yahoo.com> Unbreakable Sakura. Ren? J. --- On Fri, 10/9/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Automated H&E stainers To: histonet@lists.utsouthwestern.edu Date: Friday, October 9, 2009, 9:43 AM Which one do you like?...pro&cons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michel.Bataillon <@t> citox.com Fri Oct 9 09:28:18 2009 From: Michel.Bataillon <@t> citox.com (Bataillon Michel) Date: Fri Oct 9 09:28:25 2009 Subject: [Histonet] Automated H&E stainers References: <347591.96150.qm@web65704.mail.ac4.yahoo.com> Message-ID: <1FB9362B907EEC43959F72445AC63B6339C39A@apollon.cit_serveur.com> Hello We have also an old but unbreakable Sakura DRS-601, and we will get a new Tissue-Tek Prisma + Glas-G2 in the next weeks. Michel BATAILLON Lab Manager CIT Evreux, France michel.bataillon@citox.com -----Message d'origine----- De : histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] De la part de Rene J Buesa Envoy? : vendredi 9 octobre 2009 16:12 ? : histonet@lists.utsouthwestern.edu; Brett MConnolly Objet : Re: [Histonet] Automated H&E stainers Unbreakable Sakura. Ren? J. --- On Fri, 10/9/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Automated H&E stainers To: histonet@lists.utsouthwestern.edu Date: Friday, October 9, 2009, 9:43 AM Which one do you like?...pro&cons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************* Ce message et toutes les pi?ces jointes sont confidentiels et ?tablis ? l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le d?truire et d'en avertir imm?diatement l'exp?diteur. Toute utilisation de ce message non conforme ? sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'int?grit? de ce message, le CIT d?cline toute responsabilit? au titre de ce message s'il a ?t? alt?r?, d?form? ou falsifi?. Prot?geons ensemble l'environnement, n'imprimons ce mail (et ses pi?ces jointes) que si cela est n?cessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. ************************************************************* From Elizabeth.J.Rizzo <@t> Hitchcock.ORG Wed Oct 7 11:34:20 2009 From: Elizabeth.J.Rizzo <@t> Hitchcock.ORG (Elizabeth J. Rizzo) Date: Fri Oct 9 09:40:49 2009 Subject: [Histonet] Disinfecting Cryostat Message-ID: <67669276@mailbox3.Hitchcock.ORG> Hello Gayle, I got your name from your post on the histo listserv after I did a google search. I have a researcher who wants to use our clinical cryostat for known CJD + tissue. Have you ever used formic acid in the cryostat followed by alcohol? Where did you get supporting documentation regarding cryostat disinfection following prion exposure? Any help would be appreciated. Liz Elizabeth J. Rizzo PA(ASCP) Pathologists' Assistant Laboratory Safety Co-Chair Dartmouth-Hitchcock Medical Center Lebanon, NH 03756 603-650-7998 From carl.hobbs <@t> kcl.ac.uk Fri Oct 9 13:10:12 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Oct 9 13:14:16 2009 Subject: [Histonet] black DAB instead of brown Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742AFA@KCL-MAIL04.kclad.ds.kcl.ac.uk> Following on from Paula Pierce's apposite answer: what DAB solution are you using? A DAB kit that may well contain metal enhancers? carl From talulahgosh <@t> gmail.com Fri Oct 9 13:27:12 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Oct 9 13:33:50 2009 Subject: [Histonet] black DAB instead of brown In-Reply-To: <11D9615B89C10747B1C985966A63D7CA2991742AFA@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA2991742AFA@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: One thing (that may be a dumb idea) but our DAB kit says to use distilled water to dilute it--maybe the minerals in regular tap water are causing the black color? Our Nickel (chloride?, whatever makes the DAB a different color) comes separately, but I thought that was the case for all DAB kits. If you just have DAB (not in a kit), I don't think it usually comes with nickel in it. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum On Fri, Oct 9, 2009 at 2:10 PM, Hobbs, Carl wrote: > > Following on from Paula Pierce's apposite answer: what DAB solution are you > using? > A DAB kit that may well contain metal enhancers? > carl > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From annigyg <@t> gmail.com Fri Oct 9 22:28:51 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Oct 9 22:28:57 2009 Subject: [Histonet] Automated H&E stainers In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> Message-ID: Sakura Sakura Sakura - easy to use workhorses coupled with excellent after sales service - what more could one ever ask for!!! AbuDhabiAnnie 2009/10/9 Connolly, Brett M > Which one do you like?...pro&cons? > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or > MSD and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From Laura.Miller <@t> leica-microsystems.com Sat Oct 10 16:01:41 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Sat Oct 10 16:01:49 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 10/09/2009 and will not return until 10/13/2009. PLEASE NOTE THAT I DO NOT HAVE ACCESS TO A CELL PHONE SO IF YOU NEED IMMEDIATE ASSISANCE PLEASE CONTACT ANDREAS KAEPPLEIN. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From o.isaac24 <@t> yahoo.com Sat Oct 10 20:14:34 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Sat Oct 10 20:14:40 2009 Subject: [Histonet] NEW POSITION WANTED Message-ID: <735428.29885.qm@web111609.mail.gq1.yahoo.com> Hi, ?? I am looking for a new Histotech/IHC position. I am HTL(ASCP) certified. I am open to relocation. I have management experience? and can work as Bench Histology Supervisor, Bench Histology Manager. etc. ?Isaac. BS, HT(ASCP)HTL. From Bauer.Karen <@t> mayo.edu Mon Oct 12 07:41:51 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Mon Oct 12 07:41:56 2009 Subject: [Histonet] Automated H&E stainers In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> Message-ID: <53FC421CC200C5429929EDE6C3676F306E1571@msgebe34> Brett, We purchased the Tissue Tek PRISMA with the attached film coverslipper a year ago and LOVE IT!! Was a little nervous switching from the glass coverslips to the film, but the docs love the slides and we have had no problems. Very easy to use and we like the fact that we can file the slides the same day without having to wait for them to "dry". Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, October 09, 2009 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated H&E stainers Which one do you like?...pro&cons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrea.conard <@t> gmail.com Mon Oct 12 08:23:46 2009 From: andrea.conard <@t> gmail.com (andrea conard) Date: Mon Oct 12 08:23:49 2009 Subject: [Histonet] NYS license Message-ID: <83ddff90910120623j7d4312e0k9619d4788b3d096b@mail.gmail.com> Hi folks, I am so confused about the NYS histo license. I have been an HT(ASCP) since 1989. and want to apply for a license. The gov.website has several paths to be grandfathered in through employment history and experience but had a cut off for application of Jan. 09 for that path. Can anyone tell me if I now need to start over and go back to school or what the deal is? Thanks, Andrea From anand <@t> ethoneuro.com Mon Oct 12 08:39:00 2009 From: anand <@t> ethoneuro.com (Anand Vasudevan) Date: Mon Oct 12 08:39:26 2009 Subject: [Histonet] OX-42 Message-ID: <189449020910120639v64c2c593i7ea6bdbe4445835f@mail.gmail.com> Hi, I am doing antibody staining with mouse anti-rat Ox-42/CD11b/c (from millipore, CBL1512). I have tried staining at room temperature and at 4C but the either background staining is high (with use of detergent- 0.3% triton-x 100) or the staining is very light (with no detergent) at even 1:1000 dilution of the primary antibody. I use horse anti-mouse IgG secondary antibody and I am developing the stain with DAB. Is there anyway to increase the staining intensity other than increasing the antibody concentration? By the way I can find primary antibody of OX-42 only raised in mouse and not any other species (and all the other papers I have seen). Does anyone know the reason why? Thanks, Anand Vasudevan School of Biological Sciences NTU Singapore From tifei <@t> foxmail.com Mon Oct 12 08:50:12 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Mon Oct 12 08:50:32 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gT1gtNDI=?= References: <189449020910120639v64c2c593i7ea6bdbe4445835f@mail.gmail.com> Message-ID: <200910122150095993316@foxmail.com> i wanna the amount of ox-42 protein in microglia? I have also performed OX-42 IHC before...another company's antibody, same problem as yours. 2009-10-12 TF ???? Anand Vasudevan ????? 2009-10-12 21:47:38 ???? histonet ??? ??? [Histonet] OX-42 Hi, I am doing antibody staining with mouse anti-rat Ox-42/CD11b/c (from millipore, CBL1512). I have tried staining at room temperature and at 4C but the either background staining is high (with use of detergent- 0.3% triton-x 100) or the staining is very light (with no detergent) at even 1:1000 dilution of the primary antibody. I use horse anti-mouse IgG secondary antibody and I am developing the stain with DAB. Is there anyway to increase the staining intensity other than increasing the antibody concentration? By the way I can find primary antibody of OX-42 only raised in mouse and not any other species (and all the other papers I have seen). Does anyone know the reason why? Thanks, Anand Vasudevan School of Biological Sciences NTU Singapore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrobertson <@t> pathologysciences.com Mon Oct 12 11:04:05 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Mon Oct 12 11:04:11 2009 Subject: [Histonet] Licensing for Massachusetts? In-Reply-To: References: <422075.3161.qm@web65716.mail.ac4.yahoo.com> Message-ID: <518CD6920AA7154193CBE5977CD880733A8BD6@psmgsrv2.PSMG.local> There's no state license to practice Histology in Georgia Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor Chico, CA 95926 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Armandi, Arlene Sent: Thursday, October 08, 2009 6:57 AM To: Rene J Buesa; Histonet@lists.utsouthwestern.edu; Michelle MacVeigh-Aloni Subject: RE: [Histonet] Licensing for Massachusetts? There is no state license for Histology in California Arlene Armandi, HTL, HT(ASCP) Cedars-Sinai Medical Center Los Angeles, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 08, 2009 6:14 AM To: Histonet@lists.utsouthwestern.edu; Michelle MacVeigh-Aloni Subject: Re: [Histonet] Licensing for Massachusetts? If nothing has changed, only 12 union states and the associated state of?Puerto Rico?require a license to practice histology: California, Florida, Georgia, Hawaii, Louisiana, Montana, Nevada, New York, North Dakota, Rhode Island, Tennessee and?West Virginia. Massachusetts is not amongst them. Ren? J. --- On Wed, 10/7/09, Michelle MacVeigh-Aloni wrote: From: Michelle MacVeigh-Aloni Subject: [Histonet] Licensing for Massachusetts? To: Histonet@lists.utsouthwestern.edu Date: Wednesday, October 7, 2009, 8:11 PM Hi all, I live in Los Angeles and have HT and HTL from CA. I would like to move to Cape Cod in couple of years. I know that one needs a special Florida license to work in Fl, but do I need a special license from Massachusetts to be able to do histology there? I would appreciate any info Michelle Aloni USC Keck School of Medicine Los Angeles, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rhbrown1 <@t> histocs.com Mon Oct 12 12:14:04 2009 From: rhbrown1 <@t> histocs.com (Leroy Brown) Date: Mon Oct 12 12:20:43 2009 Subject: [Histonet] Help needed to calibrate BioGenex Optimax Message-ID: Is there someone who has done this calibration that would be willing to talk me through calibrating the X-Y calibration for my Optimax Plus stainer? It will not pick up the tips and I have recently installed the software update. It worked fine before that. Email and let me know the best time to talk to you. Thanks in advance. LeRoy Brown HT(ASCP)HTL 360-966-7300 From dr.hatemsaied <@t> yahoo.com Mon Oct 12 12:36:07 2009 From: dr.hatemsaied <@t> yahoo.com (Hatem Salim) Date: Mon Oct 12 12:36:11 2009 Subject: [Histonet] image pro plus Message-ID: <861115.89573.qm@web46111.mail.sp1.yahoo.com> ?HI ?? .I am using image pro plus to do some counting on my slides?. they are IHC slides . RGB type . I need to know how can I extract the color of interest and how can i calculte its intensity ?thank you very much ?Hatem From LBUSTAMANTE <@t> cvm.tamu.edu Mon Oct 12 12:49:27 2009 From: LBUSTAMANTE <@t> cvm.tamu.edu (Lin Bustamante) Date: Mon Oct 12 12:50:02 2009 Subject: [Histonet] Price for plastic embedding In-Reply-To: <66A13DA4.526@CVM.TAMU.EDU> References: <66A13DA4.526@CVM.TAMU.EDU> Message-ID: <4AD325DA.EB3B.00B9.1@cvm.tamu.edu> If your lab offers plastic embedding. Could you please send us your price list. Thank you very much. Lin Bustamante. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab. Supervisor Dept. of Veterinary Integrative Biosciences Texas A&M University College Station, TX 77843-4458 (979)845-3177 From CThornton <@t> dahlchase.com Mon Oct 12 13:41:29 2009 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Oct 12 13:41:33 2009 Subject: [Histonet] Folds in tissue Message-ID: We have been having ongoing issues with microscopic folds in all types of tissue. On the waterbath, the section looks neat and wrinkle free. Under the scope, there are many folds that are beginning to interfere with diagnosis. We notice the folds mostly in skin, although they have appeared in practically every type of tissue. We have been experimenting with water bath temperature; cutting on 5 microns vs. 4; baking upright in a rack or lying flat; using tissue adhesive (we use Sta-On by Surgipath) or not; and various other factors. Nothing has really jumped out as being a deciding factor. Any hints, tips, tricks, or experiments to try? What have others done when dealing with an issue like this? Thanks for all your help. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From rjbuesa <@t> yahoo.com Mon Oct 12 13:57:30 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 12 13:57:33 2009 Subject: [Histonet] Folds in tissue In-Reply-To: Message-ID: <954100.47936.qm@web65707.mail.ac4.yahoo.com> Under separate cover I am sending you?something I wrote about some Tricks of the Trade about?flotation of paraffin sections. Ren? J. --- On Mon, 10/12/09, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] Folds in tissue To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, October 12, 2009, 2:41 PM We have been having ongoing issues with microscopic folds in all types of tissue.? On the waterbath, the section looks neat and wrinkle free.? Under the scope, there are many folds that are beginning to interfere with diagnosis.? We notice the folds mostly in skin, although they have appeared in practically every type of tissue.? We have been experimenting with water bath temperature;? cutting on 5 microns vs. 4;? baking upright in a rack or lying flat;? using tissue adhesive (we use Sta-On by Surgipath) or not; and various other factors.? Nothing has really jumped out as being a deciding factor.? Any hints, tips, tricks, or experiments to try?? What have others done when dealing with an issue like this?? Thanks for all your help. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Bonnie.Whitaker <@t> osumc.edu Mon Oct 12 16:07:55 2009 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Mon Oct 12 16:08:04 2009 Subject: [Histonet] (no subject) Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D66749@msxc06.OSUMC.EDU> Hi Histonetters, I was contacted by someone in the Columbus, Ohio area who is in need of a histotech with experience in Mohs techniques. If anyone is interested, please email your contact info and a brief resume to: columbusskinsurgery@gmail.com Thanks! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 From Pathrm35 <@t> comcast.net Tue Oct 13 06:50:03 2009 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Tue Oct 13 06:50:06 2009 Subject: [Histonet] looking for Steve Westra Message-ID: <840430460.2869011255434603863.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> I'm?looking for?a contact number for Steve Westra from Leica/Vision Biosystems in south Florida. Can anyone provide one? Thanks, Ron Martin From lhotaks <@t> mcmaster.ca Tue Oct 13 12:30:14 2009 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Tue Oct 13 12:30:36 2009 Subject: [Histonet] image pro plus In-Reply-To: <200910131707.n9DH7a3p008979@zafron7.UTS.McMaster.CA> References: <200910131707.n9DH7a3p008979@zafron7.UTS.McMaster.CA> Message-ID: Hi Hatem, to separate RGB colours, go to Process/ Colour channel/ Extract. This will give you each channel in B&W, i.e. each pixel with intensity from 0 to 255 for Red, Blue and Green component of your image. There must be many ways how to do intensity. One that would be possible is: go to Process/ Pseudocolour/ Select number of divisions (bins). Intensities will then be divided into bins, say 0 to 10, 11 to 20, 21 to 30 etc. all the way to 255. And then look in areas, it will give you number of pixels for each range of intensities you selected in Divisions. Hope it helps, Sarka Lhotak, PhD McMaster University, Hamilton From nancy.troiano <@t> yale.edu Tue Oct 13 14:26:34 2009 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Tue Oct 13 14:26:40 2009 Subject: [Histonet] price for plastic embedding Message-ID: <5.2.1.1.2.20091013152448.0145d820@email.med.yale.edu> Our lab prices for plastic embedding are dependent on many variables, e.g. size of bone tissue, implant/nonimplant, services required (such as embed only, embed, cut, etc.). You can contact me for pricing for work done for outside institutions. From PMonfils <@t> Lifespan.org Tue Oct 13 14:58:46 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Oct 13 14:58:50 2009 Subject: [Histonet] Clear Paraffin? Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CEF@LSRIEXCH1.lsmaster.lifespan.org> I work in a core histology facility where I receive all kinds of specimens and embedded blocks from many different sources. Occasionally I receive blocks that are embedded in a clear (transparent, not white) embedding wax. Does anyone know what this product is? From sfeher <@t> CMC-NH.ORG Tue Oct 13 15:23:10 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Tue Oct 13 15:35:03 2009 Subject: [Histonet] Transporting Formalin Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB207F@exchange.cmc-nh.org> Hi gang, A question came up in a committee meeting today surrounding how other institutions are transporting quantities of 10% NBF from a main facility to outlying hospitals and outpatient clinics. The quantities in question ranged from 5 - 20 gallons of formalin in sealed carboy type containers. Does a main hospital or lab distribute the formalin to outlying facilities or are outlying facilities having to order direct from a supplier and bill the main facility? Some places just order it and transport via courier. This may be restricted due to various State EPA rules. How are you all doing this? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From brian <@t> prometheushealthcare.com Tue Oct 13 15:52:32 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Tue Oct 13 15:52:37 2009 Subject: [Histonet] New Lead Tech/Assistant Manager position available in NJ Message-ID: <001901ca4c47$15e77600$41b66200$@com> New Opening with a lab in Paramus Day shift 5am to 1:30pm or 6:00a to 2:30pm Stresses quality over quantity Prefers ASCP certification and NY state licensure Someone who can multitask, detail oriented IHC experience a plus Salary will commensurate upon experience Minimum 10 yrs exp Lab is growing, consistently acquiring new clients. Been in business about a year Please let me know if you may be interested! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From liljathoenes <@t> rcsi.ie Fri Oct 9 10:01:23 2009 From: liljathoenes <@t> rcsi.ie (Lilja Thoenes) Date: Tue Oct 13 16:15:09 2009 Subject: [Histonet] how to fix EGFP for proteomics Message-ID: <54E3312540DE1547A410FA8A333AC8F00223FA4089@RCSIEXCHANGE.rcsi-internal.ie> Hi , I plan isolate GFPtagged cells from cryosections via laser microdissection for proteomics but still I am struggling with not loosing the GFP signal. Does anyone has a good idea how to fix the signal so that afterwards proteomics is still possible? Thanks a lot, Lilja Lilja Thoenes Dept. of Neurodegeneration and Physiology Royal College of Surgeons in Ireland (RCSI) 123 St. Stephens Green, Dublin 2, Ireland Office: +353 1 402 2794 Fax: +353 1 402 2447 From kiran_g <@t> sbcglobal.net Sun Oct 11 23:07:39 2009 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue Oct 13 16:15:11 2009 Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress Message-ID: <277641.14522.qm@smtp101.sbc.mail.sp1.yahoo.com> Hi, Anybody has anything to share regarding Pathos both good and bad. We are planning to get one. Also please share if you have any experience with Tissue Xpress. Thanks, Histotech in CA From dphillips <@t> vetmed.lsu.edu Mon Oct 12 10:36:08 2009 From: dphillips <@t> vetmed.lsu.edu (del phillips) Date: Tue Oct 13 16:15:12 2009 Subject: [Histonet] hematoxylin and nova red staining Message-ID: <000001ca4b52$2f60e080$8e22a180$@lsu.edu> If hematoxylin is place on a slide before the nova red is it too late to add the nova red after the hematoxylin? Thanks From rhbrown1 <@t> histocs.com Tue Oct 13 16:10:39 2009 From: rhbrown1 <@t> histocs.com (Leroy Brown) Date: Tue Oct 13 16:17:25 2009 Subject: [Histonet] looking for calibration tools for Optimax plus stainer Message-ID: Does anyone have a set of calibration tools needed to calibrate my BioGenex Optimax stainer. Seems they no longer make these or sell time?? thanks LeRoy Brown Ht(ASCP) HTL www.histocs.com 360-966-7300 From ploykasek <@t> phenopath.com Tue Oct 13 16:46:37 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Oct 13 16:46:46 2009 Subject: [Histonet] looking for calibration tools for Optimax plus stainer Message-ID: In reference to LeRoy's last statement below- if anyone is selling time please put me on the list for the maximum allowable! I haven't been able to figure out how to cram more hours in a day! And it's only Tuesday. Patti Loykasek > Does anyone have a set of calibration tools needed to calibrate my BioGenex > Optimax stainer. Seems they no longer make these or sell time?? > > thanks > > LeRoy Brown Ht(ASCP) HTL > > www.histocs.com > > 360-966-7300 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From anonwums1 <@t> gmail.com Tue Oct 13 16:48:46 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Oct 13 16:48:51 2009 Subject: [Histonet] Isotype background Message-ID: <858249120910131448v26622f8dtd5599dbbf64e67e4@mail.gmail.com> Hi all, I am trying some IHC, and I am having a peculiar problem. Like I expect, my antibody of interest (anti-mouse goat polyclonal) stains nonspecifically at high concentrations (10 ug / ml) but as I titer it down (3 ug / mL), it seems to stain relatively specifically the cells I think it should stain. However, at 3 ug / mL, my isotype goat IgG stains nearly everything. Here is my protocol 1) Block in 3% H2O2 for 10'. Wash. 2) Block in 10% donkey serum for 1 hr. Wash. 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits) 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash. 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash. 7) Incubate with DAB+ (Dako) for 5'. For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson. Some people have suggested that I just do away with isotypes altogether and use a no primary control instead. I think there is some merit to this idea, but I still think my issue might be indicative of a larger technical problem in my staining protocol. Thanks, Adam From cfitz <@t> telus.net Tue Oct 13 21:10:08 2009 From: cfitz <@t> telus.net (Cathy) Date: Tue Oct 13 21:10:12 2009 Subject: [Histonet] Automated H&E stainers In-Reply-To: <53FC421CC200C5429929EDE6C3676F306E1571@msgebe34> References: <63EA0607835FBA4689CEA9EA8B4826920263C7EA@usctmx1141.merck.com> <53FC421CC200C5429929EDE6C3676F306E1571@msgebe34> Message-ID: <9EFA5FE9872A49718A169C823AD49321@your8ba846406f> Have you had any problems with the Coverslipper? We purchased the Prisma with linked Coverslipper last March and have had on going problems with the Coverslipper jamming with error code -Motor ejector error E15. We have contacted Somagen and were told that this is normal. It is happening at least once a day and sometimes more. We love the stainer and if the Coverslipper wasn't jamming we would love it just as much. What do you do with the coverslipped slides? We find that they don't dry as quickly as they did on the older film Coverslipper. We don't have a vented sign out area so they are staying on the carousel until they are mostly dry. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Monday, October 12, 2009 5:42 AM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automated H&E stainers Brett, We purchased the Tissue Tek PRISMA with the attached film coverslipper a year ago and LOVE IT!! Was a little nervous switching from the glass coverslips to the film, but the docs love the slides and we have had no problems. Very easy to use and we like the fact that we can file the slides the same day without having to wait for them to "dry". Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, October 09, 2009 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated H&E stainers Which one do you like?...pro&cons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deliadfam <@t> yahoo.com Tue Oct 13 21:52:57 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Tue Oct 13 21:53:02 2009 Subject: [Histonet] Transporting Formalin Message-ID: We have various shipping addresses with our supplier. Which allows us to order directly for our clients. We incur all costs including the associated Hazardous Materials handling fee. We also use courier for in-town clients. Hope this helps. Delia Garcia Lead Histotechnologist Tucson, AZ -----Original Message----- Date: Tuesday, October 13, 2009 1:36:33 pm To: From: "Feher, Stephen" Subject: [Histonet] Transporting Formalin Hi gang, A question came up in a committee meeting today surrounding how other institutions are transporting quantities of 10% NBF from a main facility to outlying hospitals and outpatient clinics. The quantities in question ranged from 5 - 20 gallons of formalin in sealed carboy type containers. Does a main hospital or lab distribute the formalin to outlying facilities or are outlying facilities having to order direct from a supplier and bill the main facility? Some places just order it and transport via courier. This may be restricted due to various State EPA rules. How are you all doing this? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Wed Oct 14 05:33:32 2009 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Oct 14 05:33:42 2009 Subject: [Histonet] lid gasket for processor Message-ID: Our lid gasket seemed to have grown overnight and expanded considerable in length. It is difficult to re-seat it back in. It appears too loose and when the processor cycles through a station and we open the lid, it is laying on the edge of the retort. Does anyone have any ideas what we can do until a new gasket can be ordered? Diana From melissa.mazan <@t> tufts.edu Wed Oct 14 07:22:21 2009 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Wed Oct 14 07:22:24 2009 Subject: [Histonet] pkh Message-ID: <4AD5C27D.5010601@tufts.edu> Hi all, We have collected a specific lung cell via FACS from mice, stained the cells with PKH, and then returned the cells to congenic mice via 1. tracheal injection or 2. tail vein injection. We can see the PKH positive cells in the recipient lungs on microscopy, but we are having a real problem costaining these cells with anything else such as vimentin - has anyone else tried this approach? Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu From trathborne <@t> somerset-healthcare.com Wed Oct 14 08:26:52 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Oct 14 08:26:59 2009 Subject: [Histonet] lid gasket for processor In-Reply-To: Message-ID: You might be able to shrink it a little by putting the gasket in the refrigerator for a while. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Wednesday, October 14, 2009 6:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lid gasket for processor Our lid gasket seemed to have grown overnight and expanded considerable in length. It is difficult to re-seat it back in. It appears too loose and when the processor cycles through a station and we open the lid, it is laying on the edge of the retort. Does anyone have any ideas what we can do until a new gasket can be ordered? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From rjbuesa <@t> yahoo.com Wed Oct 14 08:30:14 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 14 08:30:23 2009 Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress Message-ID: <605622.63517.qm@web65713.mail.ac4.yahoo.com> --- On Wed, 10/14/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Pathos Tissue Processor vs Tissue Xpress To: "Kiranjit Grewal" Date: Wednesday, October 14, 2009, 9:28 AM Under separate cover I am sending you an article where both instruments (as well as some others) are compared in?reurn/investment and effectiveness. Ren? J. --- On Mon, 10/12/09, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress To: histonet@lists.utsouthwestern.edu Date: Monday, October 12, 2009, 12:07 AM Hi, Anybody has anything to share regarding Pathos both good and bad. We are planning to get one. Also please share if you have any experience with Tissue Xpress. Thanks, Histotech in CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Oct 14 09:20:41 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Oct 14 09:20:46 2009 Subject: [Histonet] Isotype background In-Reply-To: <858249120910131448v26622f8dtd5599dbbf64e67e4@mail.gmail.com> References: <858249120910131448v26622f8dtd5599dbbf64e67e4@mail.gmail.com> Message-ID: <31878536799288738D402C8A@CDYwxp1931.ad.med.buffalo.edu> I wouldn't get rid of the isotype control altogether; it's fulfilling it's purpose in trying to tell you something. Have you tried a no-primary control in parallel with your isotype control to see what the secondary is binding to - the tissue or bound goat IgG? What type of tissue is it? Maybe the goat IgG is binding to Fc receptors in the tissue. Try an Fc block. Or try a different goat IgG or one from a different company. But it may not even be a big issue; your goat IgG is not giving you the specific stain pattern that you are observing with your primary, right? It's just giving a lot of background? Instead of spending a lot of time troubleshooting the high background issue with your isotype control, it's already succeeded in telling you that your primary is specific, which is what you ultimately wanted to know, so you could just take that for what it's worth and go from there... Just my two cents' worth! Regards, Merced --On Tuesday, October 13, 2009 4:48 PM -0500 "Adam ." wrote: > Hi all, > > I am trying some IHC, and I am having a peculiar problem. Like I expect, > my antibody of interest (anti-mouse goat polyclonal) stains > nonspecifically at high concentrations (10 ug / ml) but as I titer it > down (3 ug / mL), it seems to stain relatively specifically the cells I > think it should stain. However, at 3 ug / mL, my isotype goat IgG stains > nearly everything. > > Here is my protocol > 1) Block in 3% H2O2 for 10'. Wash. > 2) Block in 10% donkey serum for 1 hr. Wash. > 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits) > 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. > Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross > adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash. > 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash. > 7) Incubate with DAB+ (Dako) for 5'. > > For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson. > Some people have suggested that I just do away with isotypes altogether > and use a no primary control instead. I think there is some merit to this > idea, but I still think my issue might be indicative of a larger > technical problem in my staining protocol. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From rjbuesa <@t> yahoo.com Wed Oct 14 09:22:38 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 14 09:22:51 2009 Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress In-Reply-To: Message-ID: <158309.9167.qm@web65701.mail.ac4.yahoo.com> Hi Donna: Thank you for your comment. As you write if the Pathos Classic is still available what I wrote is still valid. Unfortunately when somebody writes an article the conclusions remain valid as long as the subjects don't change, but the approach remains. As you can see I have forwarded your comments and my answer for all to read in HistoNet so they can benefit of it. As to going to Kalamazzo I really appreciate your invitation but I seldom travel ("retired with a fixed income", you the drill!). Regards Ren? J. --- On Wed, 10/14/09, Donna Willis wrote: From: Donna Willis Subject: RE: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress To: "Rene J Buesa" Date: Wednesday, October 14, 2009, 9:58 AM Rene, There are now 2 Pathos units available to customers.? The Pathos Classic and the Pathos Delta.? You article was written before the Delta was on the market.? Please understand that giving this article to customers may not give them a true picture of the unit they are looking to purchase.? If you are interested in coming to the Milestone Kalamazoo office to experience the whole Milestone instrument line, we would love to have you. Sincerely, Donna Willis, HT/HTL(ASCP) North American Application Manager Milestone Medical (866) 995-5300 toll free (269) 488-4040 fax www.milestonemed.com Helping Patients -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, October 14, 2009 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress --- On Wed, 10/14/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Pathos Tissue Processor vs Tissue Xpress To: "Kiranjit Grewal" Date: Wednesday, October 14, 2009, 9:28 AM Under separate cover I am sending you an article where both instruments (as well as some others) are compared in?reurn/investment and effectiveness. Ren? J. --- On Mon, 10/12/09, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress To: histonet@lists.utsouthwestern.edu Date: Monday, October 12, 2009, 12:07 AM Hi, Anybody has anything to share regarding Pathos both good and bad. We are planning to get one. Also please share if you have any experience with Tissue Xpress. Thanks, Histotech in CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkwaa <@t> bidmc.harvard.edu Wed Oct 14 09:25:51 2009 From: kkwaa <@t> bidmc.harvard.edu (kkwaa@bidmc.harvard.edu) Date: Wed Oct 14 09:26:02 2009 Subject: [Histonet] HerCep Message-ID: <1453B63A4BE95441AA65F577CEE7B6E104E16997F0@EVS5CCR.its.caregroup.org> Does anyone have any experience using the Dako HerCep kit? And do you have any problems with cytoplasmic staining? From sbryant <@t> labpath.com Wed Oct 14 10:24:44 2009 From: sbryant <@t> labpath.com (susan bryant) Date: Wed Oct 14 10:25:43 2009 Subject: [Histonet] unsubscribe, please Message-ID: <4AD5ED3C.20805@labpath.com> I wish to unsubscribe and return in 6 weeks. Thank you, Susan E. Bryant Knoxville Dermatopathology From relia1 <@t> earthlink.net Wed Oct 14 10:42:07 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Oct 14 10:42:14 2009 Subject: [Histonet] RELIA Histology Job Alert Histotechnologist needed in Atlanta Message-ID: Hi Histonetters! I have a new position that I want to let everyone know about. I am assisting a client located in Atlanta GA that is in need of a histotechnologist. This is a full time permanent position on the night shift (12:30a-8:30a). My client offers excellent benefits and compensation and a generous shift differential. ASCP certification and strong experience in grossing is required. For more information please contact Pam Barker at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From talulahgosh <@t> gmail.com Wed Oct 14 11:34:59 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Oct 14 11:35:07 2009 Subject: [Histonet] unsubscribe, please In-Reply-To: <4AD5ED3C.20805@labpath.com> References: <4AD5ED3C.20805@labpath.com> Message-ID: Good for you! You should probably FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE WRITES IN TO UNSUBSCRIBE. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum On Wed, Oct 14, 2009 at 11:24 AM, susan bryant wrote: > I wish to unsubscribe and return in 6 weeks. > > Thank you, > Susan E. Bryant > Knoxville Dermatopathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carrolpb <@t> umdnj.edu Wed Oct 14 11:56:08 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Oct 14 11:56:20 2009 Subject: [Histonet] unsubscribe, please In-Reply-To: References: <4AD5ED3C.20805@labpath.com> Message-ID: <4AD602A8.6050306@umdnj.edu> >FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE WRITES IN TO UNSUBSCRIBE Histonet Fun-Fact: Hits for the word "unsubscribe" in the archive: 1144 Hits for the word "eosin" in the archive: 801 Perhaps we should rename this list "Unsubscribenet" ;) From SDrew <@t> uwhealth.org Wed Oct 14 12:09:08 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Wed Oct 14 12:09:14 2009 Subject: [Histonet] anti-H1N1 antibody? Message-ID: <738A7878143FF74BB77436E255743C1A1F7CC2@UWHC-MAIL03.uwhis.hosp.wisc.edu> One of our pathologists asked us whether we had heard on anyone having an antibody against H1N1 that could be used on formalin-fixed paraffin-embedded tissue. Anyone doing this? Or working on it? Thanks for any and all responses. Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From ploykasek <@t> phenopath.com Wed Oct 14 13:18:05 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Oct 14 13:18:21 2009 Subject: [Histonet] HerCep In-Reply-To: <1453B63A4BE95441AA65F577CEE7B6E104E16997F0@EVS5CCR.its.caregroup.org> Message-ID: Depending on the tissue fixation & processing, you can see some cytoplasmic staining. How does the cell pellet control look? We seldom see cytoplasmic staining on that. Are you tracking that your retrieval buffer is 95-100 after you add the slides & starting your retrieval times when the buffer temp gets back up to 95? Wash well between all incubations, too. That's just some thoughts off the top of my head. Let me know if you have more specific questions on the protocol. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA > Does anyone have any experience using the Dako HerCep kit? > > And do you have any problems with cytoplasmic staining? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From leiker <@t> buffalo.edu Wed Oct 14 14:09:52 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Oct 14 14:10:03 2009 Subject: [Histonet] unsubscribe, please In-Reply-To: <4AD602A8.6050306@umdnj.edu> References: <4AD5ED3C.20805@labpath.com> <4AD602A8.6050306@umdnj.edu> Message-ID: <48D72222D1A9087F3B22800C@CDYwxp1931.ad.med.buffalo.edu> LOL!!!!! Oh my I can't stop laughing...must be a serious, stressful day... --On Wednesday, October 14, 2009 12:56 PM -0400 Peter Carroll wrote: > >FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE > WRITES IN TO UNSUBSCRIBE > > Histonet Fun-Fact: > > Hits for the word "unsubscribe" in the archive: 1144 > Hits for the word "eosin" in the archive: 801 > > Perhaps we should rename this list "Unsubscribenet" ;) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From SAllen <@t> exchange.hsc.mb.ca Wed Oct 14 14:22:05 2009 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Oct 14 14:22:12 2009 Subject: [Histonet] Bielschowsky/Luxol Fast Blue stain Message-ID: Hi, Does anyone have a method for doing a combination of Bielschowsky & Luxol Fast Blue? If anyone has the method & any comments on it I would appreciate it. Thanks Sharon Allen HSC, Winnipeg, MB, CA sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From JWeems <@t> sjha.org Wed Oct 14 14:23:59 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 14 14:24:40 2009 Subject: [Histonet] unsubscribe, please In-Reply-To: <48D72222D1A9087F3B22800C@CDYwxp1931.ad.med.buffalo.edu> References: <4AD5ED3C.20805@labpath.com><4AD602A8.6050306@umdnj.edu> <48D72222D1A9087F3B22800C@CDYwxp1931.ad.med.buffalo.edu> Message-ID: Remember when we did do it that way and we had a count of how many ways you can spell it - unscribe, unsuscribe, etc! Ah the good ole days... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, October 14, 2009 15:10 To: Peter Carroll; Emily Sours Cc: histonet@lists.utsouthwestern.edu; susan bryant Subject: Re: [Histonet] unsubscribe, please LOL!!!!! Oh my I can't stop laughing...must be a serious, stressful day... --On Wednesday, October 14, 2009 12:56 PM -0400 Peter Carroll wrote: > >FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE > WRITES IN TO UNSUBSCRIBE > > Histonet Fun-Fact: > > Hits for the word "unsubscribe" in the archive: 1144 Hits for the word > "eosin" in the archive: 801 > > Perhaps we should rename this list "Unsubscribenet" ;) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From NMargaryan <@t> childrensmemorial.org Wed Oct 14 15:53:29 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Oct 14 15:55:32 2009 Subject: [Histonet] Isotype background In-Reply-To: <2df2cb49-4f65-4f28-a272-14fa0ae10e22@CMHHTCA01.childrensmemorial.org> References: <2df2cb49-4f65-4f28-a272-14fa0ae10e22@CMHHTCA01.childrensmemorial.org> Message-ID: Hi Adam, I always do the isotype control parallel with a no-primary control in parallel with my real Ab. May I suggest you to use Protein block serum free and pure Ab diluent without adding 2% donkey serum? Try this and let me know your results. If histonetters think I am wrong, fill free and please, let me know. All the best, Naira -----Original Message---------------------------------- Message: 11 Date: Tue, 13 Oct 2009 16:48:46 -0500 From: "Adam ." Subject: [Histonet] Isotype background To: histonet@lists.utsouthwestern.edu Message-ID: <858249120910131448v26622f8dtd5599dbbf64e67e4@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi all, I am trying some IHC, and I am having a peculiar problem. Like I expect, my antibody of interest (anti-mouse goat polyclonal) stains nonspecifically at high concentrations (10 ug / ml) but as I titer it down (3 ug / mL), it seems to stain relatively specifically the cells I think it should stain. However, at 3 ug / mL, my isotype goat IgG stains nearly everything. Here is my protocol 1) Block in 3% H2O2 for 10'. Wash. 2) Block in 10% donkey serum for 1 hr. Wash. 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits) 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash. 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash. 7) Incubate with DAB+ (Dako) for 5'. For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson. Some people have suggested that I just do away with isotypes altogether and use a no primary control instead. I think there is some merit to this idea, but I still think my issue might be indicative of a larger technical problem in my staining protocol. Thanks, Adam From ChaseM <@t> childrensdayton.org Wed Oct 14 16:05:36 2009 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Wed Oct 14 16:05:44 2009 Subject: [Histonet] Dayton Ohio Position Open Message-ID: <9719621676F71F49AEA7CCC6F4561B3D03E57C6CFA@PEXCHNG1.cmc-dayton.org> Hey All A fulltime Histotech position is open at Dayton Children's Hospital in Dayton Ohio. We are a small hospital. We have one part time, one full time (that could be you) and myself. We process about 5000 cases a year, we average about 15-40 blocks a day. Monday's are kinda heavy with around 70 blocks the rest of the week is easy. This is Dayton Children's Hospital, good benefits, not a whole lot of stress. If you're looking for a great place with a great group of people give me a call, or call HR at 937-641-8090 and ask for Dan Krauss. Or just apply online at http://www.childrensdayton.org/CMC_Careers/index.html If you want more specifics you can call me at 641-3000 ext 8229. Please no Headhunters, we are not allowed to use employment agencies, thanks. Matt Chase Supervisor of Pathology ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. From jmcgough <@t> clinlab.com Wed Oct 14 16:51:37 2009 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Wed Oct 14 16:51:41 2009 Subject: [Histonet] Validation of IHC In-Reply-To: <9719621676F71F49AEA7CCC6F4561B3D03E57C6CFA@PEXCHNG1.cmc-dayton.org> References: <9719621676F71F49AEA7CCC6F4561B3D03E57C6CFA@PEXCHNG1.cmc-dayton.org> Message-ID: <20091014155137.qmj7xumj7oogso4c@mail.clinlab.com> Our lab is inquiring about how other labs are validating their IHC stains. We currently are processing specimens both in a microwave and conventional processors. Are labs validating every type of program on the conventional and microwave processors? (i.e. small biopsies vs. larger tissue samples)Or just microwave vs. conventional processing? Also how many blocks of each tissue control are you testing? Thanks in advance for your replies. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com From TJJ <@t> stowers.org Wed Oct 14 16:54:04 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Oct 14 16:55:04 2009 Subject: [Histonet] Re: Isotype background Message-ID: Hi Adam, We have had the exact same experience you have sometimes when we're using Goat isotype and Rabbit isotype negative controls. Sometimes you can fiddle with the protocol and minimize some of the background, and sometimes you can't. Rabbit and goat antibodies/Igs are notoriously sticky. We use a non-serum protein blocker in our stain protocol and still have this happen. The most ideal negative control would be non-immune serum from the animals that were immunized to produce the antibody. Since most places do not make that available, we have to purchase just normal animal species isotype control and hope for the best. This might explain why your antibody is not showing the background staining, but your negative control is. Are you using any detergent in your wash buffers? You can try adding some Tween 20, 0.05% up to 0.1% or so and see if that cleans some of it up. Others might use Triton X-100, but we prefer to use Tween as it's a gentler detergent for slide-mounted samples. How many rinses are you using? We rinse 3x between steps and that should be adequate. You should be using a streptavidin block kit from Vector since you are using a conjugated streptavidin in your staining protocol. Your use of donkey serum as a blocker and as an addition in your seconday antibody should block any Fc receptor staining (in theory). Have you tried titering out the isotype control to the point of negativity? Do you ever get that? What is your dilution of your secondary antibody? Streptavidin? Could those be titered out more to get better signal/noise ratio in your primary antibody treated sample? Yes, you should try a null (no primary antibody - diluent only) control with your detection system on your sample and see if there is any background staining. But I would only use that to gauge the degree of background staining from your detection system in the absence of primary antibody. In most cases, unless there is some degree of endogenous staining, those slides are beautifully negative. Not ideal to use as a negative control other than demonstrating non-interference with the detection system. I'm sorry I don't have any specific answers. Best I can do is sympathize and offer some possible things to look at. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From lenaspencer <@t> insightbb.com Wed Oct 14 18:10:23 2009 From: lenaspencer <@t> insightbb.com (Lena Spencer) Date: Wed Oct 14 18:10:39 2009 Subject: [Histonet] PHH3 Message-ID: <000b01ca4d23$82885f90$87991eb0$@com> Anyone in histo-land who has worked up PHH3 (anti-phospo-Histone H3, mitosis marker). I would like to perform this immuno on paraffin sections that are formalin fixed, using the Ventana Benchmark XT platform. My primary workups will be one brain tumors. Thanks for any help that you may be able to provide. Lena Spencer From maria.geraci-erck <@t> spcorp.com Thu Oct 15 06:51:07 2009 From: maria.geraci-erck <@t> spcorp.com (Geraci-Erck, Maria) Date: Thu Oct 15 06:51:14 2009 Subject: [Histonet] using HEIR and PIER together in one protocol, recommendations? Message-ID: Fellow Histonetters, I am using CD90.1 to label rat tissue paraffin sections with variable results. I have tried all the conventional methods and would like to try using enzyme digestion with Heat Induced AR. Does anyone have any recommendations? Do you routinely use one retrieval before the other? Are there any references out there to refer to? I did a quick search and did not find any references for this methodology. I look forward to hearing from the Histonet. Maria Geraci-Erck Schering-Plough Research Institute Special Techniques Laboratory maria.geraci-erck@spcorp.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From W.E.J.Hoekert <@t> olvg.nl Thu Oct 15 07:10:38 2009 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Oct 15 07:10:54 2009 Subject: [Histonet] Labvision immunostainers skip slides References: <1190CB05C44B13409483514729C2FC360C0A36@PAIT42.olvg.nl> Message-ID: <1190CB05C44B13409483514729C2FC360C0A40@PAIT42.olvg.nl> Thank you all for your replies, they are of great use to me. I now understand how the skipped staining can occur. I have told our support guy to look at the reagent sensor system, and he will do so today. He told me that he has already replaced them a while ago, and that the new ones are relatively stable. However, he also told me that a few weeks ago, they changed the settings of the probe, so that the probe is now descending to the bottom of the vial when it is sucking up the reagents. So actually, I still don't understand how we can get the skipped staining. I am afraid that this problem will not be fixed very easy, if it can be fixed at all. I understand that Labvision is working on this problem and that in october next year they hope to have a solution. We have decided to deparaffinize with xylene since it seems that the PT modules are not effective enough in doing so. We will keep a closer eye on them as some of you have pointed out that they can be a cause of problems. We also have irregular staining, but this occurs also with slides that have not been treated in the PT modules. PS: we use Tris Hcl buffer (10 times concentrated) from Klinipath, which contains tween already. And yes, we do get good support, but still the problems are not solved. Thanks again, Willem Hoekert Pathology Lab, OLVG Amsterdam The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Hoekert, W.E.J. Verzonden: vr 9-10-2009 13:19 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Labvision immunostainers skip slides Dear Histonetters, A few months ago, we bought 2 Labvision Autostainers 720 from Thermo (immunostainers). We have some bad experiences with them. They seem to skip some slides every now and than (it happens maybe once every 3-4 weeks, we stain about 150 - 200 slides a day). If so, our positive controls are virtually completely negative, and the patient slides are negative as well. If this happens, we see empty 'holes' in the nuclei. Click here to see an image: http://www.immunoportal.com/modules.php?name=gallery2&g2_itemId=14523&g2_imageViewsIndex=1 All the other antibodies from the same run work fine, and if we repeat the failed cases (without changing anything) they turn out fine. Does anybody recognize this problem or knows what is going on? We dewax in PT modules (the problem occurs both with citraclere and with PT Module Buffer 1 from immunologic). I hope to hear from you. Willem Hoekert Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From Kathleen.Cormier <@t> crl.com Thu Oct 15 07:24:16 2009 From: Kathleen.Cormier <@t> crl.com (Cormier, Kathleen) Date: Thu Oct 15 07:24:14 2009 Subject: [Histonet] IHC Zinc Fixative supplier/source Message-ID: <7862ACBA8359E04FA9B0C5375A2E6F466A6CBF@shr-sv0014.na01.crl.com> Hello Netters, I am searching for a vendor to supply IHC Zinc fixative. Not zinc formalin. It's ALMOST like the BD Pharmingen IHC zinc fix, but contains Zinc acetate too. We do not want to make it up in house though....it contains calcium acetate, zinc acetate, zinc chloride and tris buffer. I have searched the 'net and all I garnered was a head ache, there are so many variations of zinc fixatives, and none are what we need. Any help would be appreciated. Thank you! Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Experience the new www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. From JCBRITTON <@t> Cheshire-Med.COM Thu Oct 15 07:36:21 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Thu Oct 15 07:36:27 2009 Subject: [Histonet] IHC Zinc Fixative supplier/source In-Reply-To: <7862ACBA8359E04FA9B0C5375A2E6F466A6CBF@shr-sv0014.na01.crl.com> References: <7862ACBA8359E04FA9B0C5375A2E6F466A6CBF@shr-sv0014.na01.crl.com> Message-ID: We use Z-Fix from Anatech LTD, 269-964-6450. Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Thursday, October 15, 2009 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Zinc Fixative supplier/source Hello Netters, I am searching for a vendor to supply IHC Zinc fixative. Not zinc formalin. It's ALMOST like the BD Pharmingen IHC zinc fix, but contains Zinc acetate too. We do not want to make it up in house though....it contains calcium acetate, zinc acetate, zinc chloride and tris buffer. I have searched the 'net and all I garnered was a head ache, there are so many variations of zinc fixatives, and none are what we need. Any help would be appreciated. Thank you! Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Experience the new www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Thu Oct 15 08:11:48 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 15 08:11:52 2009 Subject: [Histonet] Validation of IHC In-Reply-To: <20091014155137.qmj7xumj7oogso4c@mail.clinlab.com> Message-ID: <742133.75381.qm@web65706.mail.ac4.yahoo.com> If you go to HistoNet archives you will find detailed answers to your questions posted about 2 months ago. Every time you change anything in any protocol, that change has to be validated. Ren? J. --- On Wed, 10/14/09, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Validation of IHC To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 14, 2009, 5:51 PM Our lab is inquiring about how other labs are validating their IHC stains. We currently are processing specimens both in a microwave and conventional processors. Are labs validating every type of program on the conventional and microwave processors? (i.e. small biopsies vs. larger tissue samples)Or just microwave vs. conventional processing? Also how many blocks of each tissue control are you testing? Thanks in advance for your replies. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Thu Oct 15 10:05:18 2009 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Thu Oct 15 10:05:23 2009 Subject: [Histonet] Isotype background In-Reply-To: <858249120910131448v26622f8dtd5599dbbf64e67e4@mail.gmail.com> Message-ID: <891040.71825.qm@web113116.mail.gq1.yahoo.com> Your protocol is fine. It's precisely my protocol. What do you get when you do the no primary control? Although this control isn't sufficient for publication it is necessary for troubleshooting. In fact performing the following controls together can tell you exactly where the problem lies: (1) IgG control - tells you if you are getting non-specific binding due to interactions of primary with the tissue itself or with the reagents in the protocol (2) No primary, with secondary, with strepavidin, with DAB - tells you if the secondary is giving background (3) No primary, no secondary, with streptavidin, with DAB - tells you endogenous biotin background (4) No primary, no secondary, no streptavidin, with DAB - tells you endogenous peroxidase background (5) Blank - Nothing at all - just take it through staining elimination ALL reactive steps including DAB - tells you if you have endogenous pigment or hemosiderin etc in your section (6) The most important control in any experiment is the negative tissue control. This means perform your experiment specifically on tissue in which you know does not express your protein of interest. Without this control you cannot be sure what you are seeing is specific (interpreted as either your protocol has issues or the primary you are working with detects other related proteins etc). That being said, my personal experience with Chrompure IgGs is that over time they get "dirty". I am not sure whether this is due to a contamination from overuse (they sure do provide a huge amount of reagent in each vial!!) or degradation from another cause. I think it's contamination as when I aliquot them out, I get it much less than before. Also be sure you are diluting it appropriately as the stock is 11mg/ml or so and your primaries are probably 10 fold less. Is there another goat IgG you can use as a sort of "positive control". An antibody you know will stain in a certain pattern from personal experience - see if it gives you a better pattern as I think your IgG may just be "bad". You are not using BSA are you? I ask b/c goat secondaries made in donkey may cross react to bovine IgG present in BSA. Finally are you working with paraffin or frozen? --- On Tue, 10/13/09, Adam . wrote: From: Adam . Subject: [Histonet] Isotype background To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 13, 2009, 9:48 PM Hi all, I am trying some IHC, and I am having a peculiar problem. Like I expect, my antibody of interest (anti-mouse goat polyclonal) stains nonspecifically at high concentrations (10 ug / ml) but as I titer it down (3 ug / mL), it seems to stain relatively specifically the cells I think it should stain. However, at 3 ug / mL, my isotype goat IgG stains nearly everything. Here is my protocol 1) Block in 3% H2O2 for 10'. Wash. 2) Block in 10% donkey serum for 1 hr. Wash. 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits) 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash. 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash. 7) Incubate with DAB+ (Dako) for 5'. For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson. Some people have suggested that I just do away with isotypes altogether and use a no primary control instead. I think there is some merit to this idea, but I still think my issue might be indicative of a larger technical problem in my staining protocol. Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Oct 15 10:55:47 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Oct 15 10:55:56 2009 Subject: [Histonet] RELIA Solutions Histology Careers Bulletin 10/15/09 Message-ID: Hi Histonetters!! I hope you are enjoying a wonderful Autumn. Halloween is just around the corner TRICK or TREAT!!!!! The TRICK is finding the right job opportunity for you. And the TREAT is with my help it can be relatively painless. I will help you with your resume, coach you through the interview and offer process and refer you to positions based on the critieria you give me. Here are my current histology openings. All of the positions I work with are fulltime 40 hour per week positions with top hospitals, labs and doctors offices. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance and in some cases sign on bonuses. My services are FREE of charge to you. All of my fees are paid by my clients, (the facilities that I represent). I represent companies nationwide that are in need of histology supervisors, histotechnologists and histotechnicians. Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA ? Central CA ? Pathology Supervisor TX- Austin ? Histology Supervisor CA ? Los Angeles ?Histology Supervisor MA ? Cape Cod Pathology Supervisor HISTOTECHS GA ? Atlanta Histotechnologist w/grossing ? Night Shift AZ ? Tucson area Histotech brand new lab CA ? San Francisco Bay Area, Research Investigator (PhD) PA- Pittsburgh, HT or HTL required. MA ? North Shore of Boston ? Histotech 2nd or 3rd shift NY-Orange/Rockand County Brand New Lab NYS license req NY-Upstate NY NYS license req NY-NYC night shift NYS license req FL- Largo part time FL lic req RESEARCH CA ? San Francisco Bay Area, Research Investigator (PhD) If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Happy Halloween!!!!!!!!!!!! Pam ? 866-607-3542 (866-60RELIA) p.s. What are you going to be for Halloween? Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia From gayle.callis <@t> bresnan.net Thu Oct 15 11:03:01 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Oct 15 11:03:17 2009 Subject: [Histonet] IHC Zinc Fixative supplier/source Message-ID: <000001ca4db0$fa3858a0$eea909e0$@callis@bresnan.net> Concerning IHC zinc fixative supplier/source FIRST, Z-Fix from Anatech is NOT the formalin free Beckstead Zinc fixative (BD Pharmingen) commonly used for rodent and human CD/leukocyte marker work. Kathy wrote: I am searching for a vendor to supply IHC Zinc fixative. Not zinc formalin. It's ALMOST like the BD Pharmingen IHC zinc fix, but contains Zinc acetate too. We do not want to make it up in house though....it contains calcium acetate, zinc acetate, zinc chloride and tris buffer. I have searched the 'net and all I garnered was a head ache, there are so many variations of zinc fixatives, and none are what we need. Any help would be appreciated. Thank you! ***************************************************** Kathy, What you are looking for is the true, original formulation of the Beckstead Zinc Tris buffer (non-formalin) fixative. The BD Biosciences Zinc Fixative (Formalin Free) Cat# 552658 technical data sheet cites his publication, but the MSDS shows zinc acetate is not part of the reported toxic substances. If they are citing Beckstead, they are more than likely using his original formulation (see below) but to make sure this is absolutely correct, you should contact their technical services about this. They have zinc in the recipe from zinc chloride and maybe they have modified the formulation so that it works as well or better from the original without the zinc acetate. Making it up in house was not difficult when we tried the fixative. Zinc Fixative (JB Fixative or ZSF) 0.1M Tris Buffer, pH 7.4 Tris Base -------------------------------- 12.1 g (TRIZMA) 1N HCL ----------------------------------- 81.5 ml Distilled water -------------------------- 900 ml Mix to dissolve. Adjust pH to 7.4 Zinc Fixative Calcium Acetate ---------------------- 0.5 g Zinc Acetate -------------------------- 5.0 g Zinc Chloride -------------------------- 5.0 g 0.1M Tris Buffer made above ------ 1000 ml Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution. Store Zinc Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation longer than 48 hours may make the tissue brittle and difficult to cut. As far as I know, there is no other company in the US that makes up this fixative - a unique one of a kind fixative not commonly used by many labs except maybe research facililites. I think it is available from sources in Europe, but can't be sure of exactly what they are selling from publications I have read. Sorting that out was not fun. Personally, I would trust the BD Bioscience Zinc fixative (formalin free) simply because they do cite Beckstead's publication. I know that Ray Koelling, now at Phenopath, has used this fixative in the past, and he may have purchased it from BD. Hopefully he is looking in and can address your problem. He has been CC'd with this message. Good luck and hope your headache goes away - Gayle M. Callis From rsrichmond <@t> gmail.com Thu Oct 15 12:24:42 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Oct 15 12:24:45 2009 Subject: [Histonet] Re: IHC Zinc Fixative supplier/source Message-ID: Gayle Callis - thanks for the IHC zinc fixative brew - promptly copied into my permanent files on the subject. Bob Richmond From koellingr <@t> comcast.net Thu Oct 15 13:18:03 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Oct 15 13:18:07 2009 Subject: [Histonet] IHC Zinc Fixative supplier/source In-Reply-To: <000001ca4db0$fa3858a0$eea909e0$@callis@bresnan.net> Message-ID: <398414034.3120891255630683090.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Hi Gayle, I've used this extensively. But never from BD. Was too simple to make in house and then we had control over tweaking it if needed. Made it up per the formula you wrote and with all your caveats and warnings with which I completely agree. We loved it for certain things. For instance, was NOT good on tissues that were loaded with digestive enzymes-like pancreas. Pancreatic tissue looked like road kill although islet of L cells looked and stained good. Although given the moniker of "fixative" it certainly isn't..in the classical sense of a fixative. And as you alluded to it is available in Europe. One of our post-docs worked with the company when in Europe. But expensive beyond reason. So we just made it. Ray Raymond Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "gayle callis" To: histonet@lists.utsouthwestern.edu Cc: koellingr@comcast.net Sent: Thursday, October 15, 2009 9:03:01 AM GMT -08:00 US/Canada Pacific Subject: [Histonet] IHC Zinc Fixative supplier/source Concerning IHC zinc fixative supplier/source FIRST, Z-Fix from Anatech is NOT the formalin free Beckstead Zinc fixative (BD Pharmingen) commonly used for rodent and human CD/leukocyte marker work. Kathy wrote: I am searching for a vendor to supply IHC Zinc fixative. Not zinc formalin. It's ALMOST like the BD Pharmingen IHC zinc fix, but contains Zinc acetate too. We do not want to make it up in house though....it contains calcium acetate, zinc acetate, zinc chloride and tris buffer. I have searched the 'net and all I garnered was a head ache, there are so many variations of zinc fixatives, and none are what we need. Any help would be appreciated. Thank you! ***************************************************** Kathy, What you are looking for is the true, original formulation of the Beckstead Zinc Tris buffer (non-formalin) fixative.? The BD Biosciences Zinc Fixative (Formalin Free) Cat# 552658 technical data sheet cites his publication, but the MSDS shows zinc acetate is not part of the reported toxic substances. If they are citing Beckstead, they are more than likely using his original formulation (see below) but to make sure this is absolutely correct, you should contact their technical services about this.? They have zinc in the recipe from zinc chloride and maybe they have modified the formulation so that it works as well or better from the original without the zinc acetate. Making it up in house was not difficult when we tried ?the fixative. Zinc Fixative (JB Fixative or ZSF) 0.1M Tris Buffer, pH 7.4 Tris Base -------------------------------- 12.1 g (TRIZMA) 1N HCL ----------------------------------- 81.5 ml Distilled water -------------------------- 900 ml Mix to dissolve. Adjust pH to 7.4 Zinc Fixative Calcium Acetate ---------------------- 0.5 g Zinc Acetate -------------------------- 5.0 g Zinc Chloride -------------------------- 5.0 g 0.1M Tris Buffer made above ------ 1000 ml Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution. Store Zinc Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation longer than 48 hours may make the tissue brittle and difficult to cut. As far as I know, there is no other company in the US that makes up this fixative - a unique one of a kind fixative not commonly used by many labs except maybe research facililites. I think it is available from sources in Europe, but can't be sure of exactly what they are selling from publications I have read. Sorting that out was not fun. Personally, I would trust the BD Bioscience Zinc fixative (formalin free) simply because they do cite Beckstead's publication. I know that Ray Koelling, now at Phenopath, has used this fixative in the past, and he may have purchased it from BD. Hopefully he is looking in and can address your problem. He has been CC'd with this message. Good luck and hope your headache goes away - Gayle M. Callis From Maria.Katleba <@t> stjoe.org Thu Oct 15 14:02:26 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Oct 15 14:03:27 2009 Subject: [Histonet] Leica Tissue Processor- Issue with Eosin in alcohol Message-ID: <97C02552ECB11346877D3E83CF833ABD13EDC63ADC@SJSNT-SCMAIL03.stjoe.org> Hi All, Have any of you had FAILURE of valves or downtime due to over use of Eosin in the Leica Asp 3000? I used to use 1-2 mls, never had a problem. The Histotech that is no longer here (do the math) trained my new person to literally "pour" in the eosin. So possibly 10-30mls are going into each bottle. Is that enough to 'make the valves stick'? Please advise if you have had or heard of this problem. Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From rjbuesa <@t> yahoo.com Thu Oct 15 14:14:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 15 14:14:44 2009 Subject: [Histonet] Leica Tissue Processor- Issue with Eosin in alcohol In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13EDC63ADC@SJSNT-SCMAIL03.stjoe.org> Message-ID: <592150.87527.qm@web65701.mail.ac4.yahoo.com> Not likely. The eosin in ethanol has no special qualities that will make a valve to get stuck. Try to find out other possible reason. Ren? J. --- On Thu, 10/15/09, Maria Katleba wrote: From: Maria Katleba Subject: [Histonet] Leica Tissue Processor- Issue with Eosin in alcohol To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, October 15, 2009, 3:02 PM Hi All, Have any of you had FAILURE of valves or downtime due to over use of Eosin in the Leica Asp 3000? I used to use 1-2 mls, never had a problem.? The Histotech that is no longer here (do the math) trained my new person to literally "pour" in the eosin. So possibly 10-30mls are going into each bottle.? Is that enough to 'make the valves stick'? Please advise if you have had or heard of this problem. Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 15 14:15:36 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 15 14:15:40 2009 Subject: [Histonet] Leica Tissue Processor- Issue with Eosin in alcohol In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13EDC63ADC@SJSNT-SCMAIL03.stjoe.org> Message-ID: <44241.40942.qm@web65713.mail.ac4.yahoo.com> Not likely! --- On Thu, 10/15/09, Maria Katleba wrote: From: Maria Katleba Subject: [Histonet] Leica Tissue Processor- Issue with Eosin in alcohol To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, October 15, 2009, 3:02 PM Hi All, Have any of you had FAILURE of valves or downtime due to over use of Eosin in the Leica Asp 3000? I used to use 1-2 mls, never had a problem.? The Histotech that is no longer here (do the math) trained my new person to literally "pour" in the eosin. So possibly 10-30mls are going into each bottle.? Is that enough to 'make the valves stick'? Please advise if you have had or heard of this problem. Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Oct 15 15:48:21 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Oct 15 15:48:27 2009 Subject: [Histonet] CPT coding clarification Message-ID: <4AD75254.7400.0077.1@harthosp.org> If a clinician puts four GI biopsies (from different sites) into one formalin container, resulting in one paraffin block, I know that there can only be one 88305-TC charge. However, can the pathologist bill 88305-26x4 for each individual tissue specimen? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From JWeems <@t> sjha.org Thu Oct 15 15:51:22 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 15 15:51:37 2009 Subject: [Histonet] CPT coding clarification In-Reply-To: <4AD75254.7400.0077.1@harthosp.org> References: <4AD75254.7400.0077.1@harthosp.org> Message-ID: Only if the sites can be separately identified. But the technical and professional must equal, so if they can be separately identified, then there would be four 88305s. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, October 15, 2009 16:48 To: Histonet Subject: [Histonet] CPT coding clarification If a clinician puts four GI biopsies (from different sites) into one formalin container, resulting in one paraffin block, I know that there can only be one 88305-TC charge. However, can the pathologist bill 88305-26x4 for each individual tissue specimen? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From AFoshey <@t> chw.org Thu Oct 15 15:54:18 2009 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Thu Oct 15 15:54:23 2009 Subject: [Histonet] Procedure for manual C4D immunofluorescence staining Message-ID: Histonetters, Does anyone have a procedure they could share for manual C4D immunofluorescence staining? Thanks in advance Annette Foshey, HT (ASCP) Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org From burch007 <@t> mc.duke.edu Thu Oct 15 16:08:36 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Oct 15 16:08:47 2009 Subject: [Histonet] Procedure for manual C4D immunofluorescence staining In-Reply-To: Message-ID: Frozen sections cut at 3-4 microns. Air dry 30 minutes. Acetone fix for 5 minutes (room temp acetone), air dry slides PBS wash x2 C4d (diluted 1:500 in 1% BSA/PBS) incubated 1 hour. Mouse monoclonal C4d is from AbDirect/Serotec PBS rinse, wash x2 Cy2 labeled goat anti-mouse IgG (diluted 1:100 in 1% BSA/PBS), incubated 30 minutes (Cy2 GAR from Jackson ImmunoResearch Labs) PBS rinse, wash x2 Coverslip with fluorescence mounting media Happy viewing I run this protocol everyday! Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Foshey, Annette" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/15/2009 04:56 PM To " (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] Procedure for manual C4D immunofluorescence staining Histonetters, Does anyone have a procedure they could share for manual C4D immunofluorescence staining? Thanks in advance Annette Foshey, HT (ASCP) Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Oct 15 16:10:56 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Oct 15 16:11:01 2009 Subject: [Histonet] NSH Website down Message-ID: <44079.24481.qm@web113810.mail.gq1.yahoo.com> Hi, ?I just tried to get into the NSH website and it's down. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com From jcampbell <@t> vdxpathology.com Thu Oct 15 19:36:41 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Thu Oct 15 19:36:48 2009 Subject: [Histonet] Rabbit-on-Rodent HRP polymer Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FB5@VDXSERVER01.vdxpathology.local> Hi All, I recently purchased a rabbit-on-rodent HRP polymer from Biocare. I will be using this on mouse tissue, using a rabbit polyclonal CD3 as my primary. I just wanted to clarify a couple things about the protocol before I try it out tomorrow and was wondering if anyone could offer some assistance. First of all, I should aske if anyone has a protocol for this? What I really would like to make sure is that I will not have to use a label after the rabbit-on-rodent secondary ab. I believe that I will just have to apply my blocking agent, avidin/biotin blocks, primary antibody, secondary anitbody, followed by chromogen, right? My blocking agent is made up of casein and some other proprietary agents in a phosphate buffer (it is non-serum) so I was told that should work fine. Does this sound correct? Thanks in advance! I haven't been able to get through to tech support all day, so hopefully you will have some answers for me! Jen From annigyg <@t> gmail.com Fri Oct 16 00:17:32 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Oct 16 00:17:38 2009 Subject: [Histonet] Procedure for manual C4D immunofluorescence staining In-Reply-To: References: Message-ID: thanks for this method we have been battling to get C4d FITC to work hopefully i can get a vendor here in the desert to source the reagents (that is a huge problem here) AbuDhabiAnnie 2009/10/16 James L Burchette > Frozen sections cut at 3-4 microns. Air dry 30 minutes. > Acetone fix for 5 minutes (room temp acetone), air dry slides > PBS wash x2 > C4d (diluted 1:500 in 1% BSA/PBS) incubated 1 hour. Mouse monoclonal C4d > is from AbDirect/Serotec > PBS rinse, wash x2 > Cy2 labeled goat anti-mouse IgG (diluted 1:100 in 1% BSA/PBS), incubated > 30 minutes (Cy2 GAR from Jackson ImmunoResearch Labs) > PBS rinse, wash x2 > Coverslip with fluorescence mounting media > Happy viewing > > I run this protocol everyday! > > Jim Burchette, HT(ASCP) QIHC > "A simple histotech from a little country hospital in North Carolina" > > > > "Foshey, Annette" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/15/2009 04:56 PM > > To > " (histonet@lists.utsouthwestern.edu)" > cc > > Subject > [Histonet] Procedure for manual C4D immunofluorescence staining > > > > > > > Histonetters, > Does anyone have a procedure they could share for manual C4D > immunofluorescence staining? > > Thanks in advance > > Annette Foshey, HT (ASCP) > Charge Tech in Histology > Children's Hospital of Wisconsin > 414-266-6580 Fax 414-266-2779 > afoshey@chw.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From jcampbell <@t> vdxpathology.com Fri Oct 16 09:02:48 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Fri Oct 16 09:02:52 2009 Subject: [Histonet] rabbit-on-rodent HRP polymer--Ready-to-use? Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82AC5EA@VDXSERVER01.vdxpathology.local> Hi All, I had posted a question about the rabbit-on-rodent HRP polymer from Biocare yesterday and I was just wondering if anyone could tell me whether or not this is RTU or can be diluted. It doesn't specify anywhere on the package insert. Thanks, Jennifer From alyssa <@t> alliedsearchpartners.com Fri Oct 16 09:48:25 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Oct 16 09:48:29 2009 Subject: [Histonet] Private Lab Needs Histotech-ATL, GA Message-ID: Allied Search Partners is now accepting resumes for our client of Atlanta, GA. We are accepting the following resumes for permanent/direct hire positions: Position: Histotechnicians/Histotechnologists Shifts: Day Shift, 7:30am-4:30pm (Hours Flexible) Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. Location: Private Lab in Atlanta, GA -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From dr.hatemsaied <@t> yahoo.com Fri Oct 16 09:56:40 2009 From: dr.hatemsaied <@t> yahoo.com (Hatem Salim) Date: Fri Oct 16 09:56:44 2009 Subject: [Histonet] osteoblast count Message-ID: <897230.82631.qm@web46105.mail.sp1.yahoo.com> HI ?? I have a problem with counting osteoblast in IHC staining slides is there a way to differentiate osteoblast from other bone cell types. thank you very much ? ? Hatem From Allison_Scott <@t> hchd.tmc.edu Fri Oct 16 10:02:34 2009 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Oct 16 10:02:42 2009 Subject: [Histonet] Processing Whole prostate Message-ID: <1872B4A455B7974391609AD8034C79FC8BD626@LBEXCH01.hchd.local> Hello to all in histoland. For those who are doing whole mounts for prostate, be willing to share the process with me. My lab is looking into to doing this. I would need to know the euipment used and processing times. Your help in this matter would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 7135665287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Shin-Young.Park <@t> childrens.harvard.edu Fri Oct 16 10:03:45 2009 From: Shin-Young.Park <@t> childrens.harvard.edu (Park, Shin-Young) Date: Fri Oct 16 10:03:58 2009 Subject: [Histonet] Nuclear DAPI staining in frozen sections of bones Message-ID: <30D1A2CD76E4BB4A81640E95CCB38B9F41AB76FAD4@CHEXCCRV1.CHBOSTON.ORG> I am doing nuclear DAPI staining in frozen sections of fresh, non-decalcified mouse femurs. The fresh femurs were snap frozen in OCT block by 2-methylbutane (isopentane)/dry ice. Cryosection of the bones were done by using the Instrumedics CryoJane tape transfer system. After transferring sections to slides, the slides were kept in -80oC until staining. But I have problem with the quality of nuclear DAPI staining, although H&E staining is pretty good. The nuclear DAPI staining (Vectorshield with DAPI or 1 uM DAPI, 10 min in PBS) is fussy and unclear. It is hard to identify each individual cell by nuclear staining. If anyone else has experience on DAPI staining in frozen bone sections, could you share any suggestion to improve the DAPI staining? Any help would be greatly appreciated. Shin-Young Park From ratliffjack <@t> hotmail.com Fri Oct 16 10:31:22 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Oct 16 10:31:27 2009 Subject: [Histonet] osteoblast count In-Reply-To: <897230.82631.qm@web46105.mail.sp1.yahoo.com> References: <897230.82631.qm@web46105.mail.sp1.yahoo.com> Message-ID: I am not sure about after staining with IHC, but if you stain with a toluidine blue or MacNeal's tetrachrome you can easily identify osteoblasts. Jack > Date: Fri, 16 Oct 2009 07:56:40 -0700 > From: dr.hatemsaied@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] osteoblast count > > HI > > I have a problem with counting osteoblast in IHC staining slides is there a way to differentiate osteoblast from other bone cell types. > thank you very much > > Hatem > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thachdc <@t> niaid.nih.gov Fri Oct 16 10:55:15 2009 From: thachdc <@t> niaid.nih.gov (Thach, Dzung (NIH/NIAID) [E]) Date: Fri Oct 16 10:55:19 2009 Subject: [Histonet] Perfect perfusion Message-ID: Hi Everyone!! Is there a way to get perfect perfusion for each mouse every time? I am getting variable perfusion quality for each mouse. I am perfusing CO2 euthanized 3-4 weeks old mice with PBS via peristaltic pump followed by fixative (RNAlater) via syringe merging into the same tubing for PBS. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle. I am collecting brain and spinal cord. Thanks much, Dzung NIAID From TJJ <@t> stowers.org Fri Oct 16 12:14:47 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Oct 16 12:15:41 2009 Subject: [Histonet] Re: Rabbit-on-Rodent HRP polymer Message-ID: Jennifer, You will not need to use the avidin/biotin block in your protocol. The polymer technology does not use any sort of biotinylation or avidin system for staining. That should help knock some time off your protocol. You will need to make sure you use a peroxidase block (3% hydrogen peroxide) in your staining protocol to block endogenous peroxidase activity (namely red blood cells/sera). Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From david.l.krull <@t> gsk.com Fri Oct 16 13:03:48 2009 From: david.l.krull <@t> gsk.com (david.l.krull@gsk.com) Date: Fri Oct 16 13:04:04 2009 Subject: [Histonet] Rabbit-onRodent HRP Polymer Message-ID: Hi Jen, The rabbit-on-rodent HRP ploymer is ready-to-use. In my protocol I depar and hydrate to wash buffer then block for endogenous peroxidase for 10 minutes using Dako's DEEB followed by wash buffer rinses then block for 15 minutes in Rodent Block M (Biocare). No need to block for biotin since no streptavidin is used in the detection. I suggest that you first try the block you have. Apply rabbit primary antibody for 30 minutes, rinse in wash buffer, then apply the rodent-on-rabbit HRP polymer for 15 to 30 minutes. Rinse in wash buffer again then apply your DAB chromagen for 5 minutes. Rinse well in DI water then stain with hematoxylin. I run all of my IHC on a Lieca Bond Max using the Biocare reagents and they work great. This protocol intended for general guidance; the exact conditions will need to be worked out in your lab. Hope this helps. Dave Jen wrote: Message: 11 Date: Thu, 15 Oct 2009 17:36:41 -0700 From: "Jennifer Campbell" Subject: [Histonet] Rabbit-on-Rodent HRP polymer To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FB5@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" Hi All, I recently purchased a rabbit-on-rodent HRP polymer from Biocare. I will be using this on mouse tissue, using a rabbit polyclonal CD3 as my primary. I just wanted to clarify a couple things about the protocol before I try it out tomorrow and was wondering if anyone could offer some assistance. First of all, I should aske if anyone has a protocol for this? What I really would like to make sure is that I will not have to use a label after the rabbit-on-rodent secondary ab. I believe that I will just have to apply my blocking agent, avidin/biotin blocks, primary antibody, secondary anitbody, followed by chromogen, right? My blocking agent is made up of casein and some other proprietary agents in a phosphate buffer (it is non-serum) so I was told that should work fine. Does this sound correct? Thanks in advance! I haven't been able to get through to tech support all day, so hopefully you will have some answers for me! Jen From awatanabe <@t> tgen.org Fri Oct 16 13:09:34 2009 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Fri Oct 16 13:09:39 2009 Subject: [Histonet] Oil Red O protocol Message-ID: I?m looking for a protocol for Oil Red O that does not use propylene glycol to make the Oil Red O. Does anyone have a protocol that I could use? Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From plucas <@t> biopath.org Fri Oct 16 13:13:50 2009 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Oct 16 13:13:54 2009 Subject: [Histonet] Saturday Tech Needed- Orange County California Message-ID: <20091016181350.22583367D@theseus.cnchost.com> Hello, Private lab seeking a histoch to work on Saturdays. Will be rotating with the other Saturday techs. Shift starts at 5 or 6 am. If interested, please send me an email. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 From Sharron.Ladd <@t> on-q-ity.com Fri Oct 16 14:51:13 2009 From: Sharron.Ladd <@t> on-q-ity.com (Sharron Ladd) Date: Fri Oct 16 14:56:32 2009 Subject: [Histonet] Need maintenance/service on Microm HM340E-2 microtome in Waltham, MA please Message-ID: Hello Histonet, I am looking for a company other than Thermo Fisher to do annual maintenance and repairs on our Microm HM340E-2 microtome. Please email or call me as soon as possible. Emailing your price list would be very helpful. Here are the details: It is 2 years old but virtually brand new (hardly used until recently). It's been working fine, then I moved it from one room to the next yesterday and today I am getting a strange venetian blind pattern (first section ok, second section ok, 3rd section bottom third venetian blind, 4th section bottom half venetian blind, 5th and 6th sections all venetian blind and then it's fine again and it repeats the pattern all over). I'm using a paraffin block with no tissue in it to trouble shoot. Section thickness: 4 microns Blades: DuraEdge high profile, same package that was working fine, new blade (tried 4 different ones) Angle: 8 degrees (unchanged)-tried 10 and it made no difference Cold block: yes -I checked if everything was tight. -I tried the blade holder tightener at 3 different spots. When I loosened it I got thick and thin sections. Nothing feels loose! I think whatever is wrong is inside (a spring, or the mechanism that keeps section thickness at 4 microns-at 5 microns the pattern seems to go away??) I look forward to your responses. Thank you and Happy Friday. Sharron Ladd Senior Histotechnologist On-Q-ity 610 Lincoln Street, 3rd Floor Waltham, MA 02451 Phone: (781) 895-8110 Fax: (781) 890-4636 sharron.ladd@on-q-ity.com From rjbuesa <@t> yahoo.com Sat Oct 17 09:33:31 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 17 09:33:36 2009 Subject: [Histonet] Processing Whole prostate In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD626@LBEXCH01.hchd.local> Message-ID: <869444.78844.qm@web65712.mail.ac4.yahoo.com> The important aspect is fixation. Let your PA slice the whole prostate and place them in the so called "mega-cassettes" in a plastic container over a vortex so they rotate inside the NBF for at least 24 hours. After that just use the longest of your protocols. If you have one that has worked well for brain tissue, use that one. Ren? J. --- On Fri, 10/16/09, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Processing Whole prostate To: histonet@lists.utsouthwestern.edu Date: Friday, October 16, 2009, 11:02 AM Hello to all in histoland.? For those who are doing whole mounts for prostate,? be willing to share the process with me.? My lab is looking into to doing this.? I would need to know the euipment used and processing times.? Your help in this matter would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 7135665287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rsrichmond <@t> gmail.com Sat Oct 17 15:56:37 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Oct 17 15:56:40 2009 Subject: [Histonet] Re: Processing Whole prostate Message-ID: I don't have any experience with whole-mount sections of radical prostatectomy specimens, which obviously require slides larger than the standard 3 by 1 inch slide. I'm not entirely current here, but a few years ago major prostate pathologists such as Jonathan Epstein (at Johns Hopkins) were telling us that while whole-mount sections were necessary for in research settings such as his own, that they were distinctly not required as standard of care in community hospitals. I prefer to let a whole radical prostatectomy specimen sit overnight in neutral buffered formalin (without removing the urethral catheter if there is one), then cut it, then further fix the sections overnight. Fixation isn't wonderful, but remember that most of what I want to see is near the exterior surfaces of the specimen. I flick out any of those little black prostatic calculi I may see. The right and left halves of the prostate should be marked with contrasting colors of ink, either before or after fixation, but before cutting. Learning to orient the specimen is a non-trivial task. I wouldn't invest much money in setting up whole-mount sections. I expect the numbers of these specimens to decrease, as the futility of treating early prostate cancer is more widely appreciated. Bob Richmond Samurai Pathologist Knoxville TN From khicks71 <@t> comcast.net Sun Oct 18 13:45:11 2009 From: khicks71 <@t> comcast.net (khicks71@comcast.net) Date: Sun Oct 18 13:45:14 2009 Subject: [Histonet] MART-1 Message-ID: <1987930656.5150001255891511006.JavaMail.root@sz0061a.emeryville.ca.mail.comcast.net> I am trying to work up a protocol for MART-1 for the Mohs surgeon I currently work for.? I have the antibody from Thermo-Shandon and also have their kit. I get positive staining but it is very faint. Any suggestions would be helpful. Thanks, Kathy Hicks H.T.(ASCP) From nickandmanda <@t> paradise.net.nz Sun Oct 18 22:59:33 2009 From: nickandmanda <@t> paradise.net.nz (Nick and Amanda) Date: Sun Oct 18 23:00:05 2009 Subject: [Histonet] Re: Histonet Digest, Vol 71, Issue 18 References: <0KRP000X7ZD0FO90@mx7.clear.net.nz> Message-ID: <002401ca5070$9137e880$c8084979@bow1> Hi can anyone please tell me who supplies Pgfr antibody? Manda in Wellington ----- Original Message ----- From: To: Sent: Monday, October 19, 2009 6:03 AM Subject: Histonet Digest, Vol 71, Issue 18 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Processing Whole prostate (Robert Richmond) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 17 Oct 2009 16:56:37 -0400 > From: Robert Richmond > Subject: [Histonet] Re: Processing Whole prostate > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't have any experience with whole-mount sections of radical > prostatectomy specimens, which obviously require slides larger than > the standard 3 by 1 inch slide. > > I'm not entirely current here, but a few years ago major prostate > pathologists such as Jonathan Epstein (at Johns Hopkins) were telling > us that while whole-mount sections were necessary for in research > settings such as his own, that they were distinctly not required as > standard of care in community hospitals. > > I prefer to let a whole radical prostatectomy specimen sit overnight > in neutral buffered formalin (without removing the urethral catheter > if there is one), then cut it, then further fix the sections > overnight. Fixation isn't wonderful, but remember that most of what I > want to see is near the exterior surfaces of the specimen. I flick out > any of those little black prostatic calculi I may see. > > The right and left halves of the prostate should be marked with > contrasting colors of ink, either before or after fixation, but before > cutting. Learning to orient the specimen is a non-trivial task. > > I wouldn't invest much money in setting up whole-mount sections. I > expect the numbers of these specimens to decrease, as the futility of > treating early prostate cancer is more widely appreciated. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 71, Issue 18 > **************************************** > From Melanie.Black <@t> uct.ac.za Mon Oct 19 01:28:34 2009 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Mon Oct 19 01:30:12 2009 Subject: [Histonet] Rabbit-on-Rodent HRP polymer Message-ID: Hi Jen In reply to your question on the Biocare Rabbit-on rodent HRP, you are correct. I include the protocol I use, the only difference is mine is Alp phos linked instead of HRP. I use rat tissue and CD 31 antibody (from Fitzgerald) that specifically requires zinc fixed tissue. For the zinc fixation, I use exactly the same formula that Gayle Callis sent out. CD 31 Alk Phos Method for Zinc fixed Tissue. Biocare Medical Kit. Dewax slides in xylene. Take slides thru alcohol to water, and into tris buffer. Incubate with Rodent Block R (RBR962H) from Biocare Medical, for 30 mins. Wash in TBS X 2. Incubate with Primary antibody for 30 mins.(1:50) ? diluted in 1% BSA in PBS. Anti Rat CD 31 from Fitzgerald. Wash in TBS X 2. Incubate in Mouse-on-Rat AP-Polymer (MRT623H), Biocare Medical, for 20 mins. Wash in TBS X 2. Add a drop of NBT(DAKO ? K0598), and check for colour development. (YOU WILL USE DAB) Rinse in water, dehydrate clear and mount. NB: Sections must be cut onto UNCOATED slides, and incubated on a 60 degree hot plate for 30 mins, followed by overnight incubation at 37 degrees. Hope this helps Regards Melanie. Hi All, I recently purchased a rabbit-on-rodent HRP polymer from Biocare. I will be using this on mouse tissue, using a rabbit polyclonal CD3 as my primary. I just wanted to clarify a couple things about the protocol before I try it out tomorrow and was wondering if anyone could offer some assistance. First of all, I should aske if anyone has a protocol for this? What I really would like to make sure is that I will not have to use a label after the rabbit-on-rodent secondary ab. I believe that I will just have to apply my blocking agent, avidin/biotin blocks, primary antibody, secondary anitbody, followed by chromogen, right? My blocking agent is made up of casein and some other proprietary agents in a phosphate buffer (it is non- serum) so I was told that should work fine. Does this sound correct? Thanks in advance! I haven't been able to get through to tech support all day, so hopefully you will have some answers for me! Jen Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From Kjones <@t> upei.ca Mon Oct 19 06:50:10 2009 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Mon Oct 19 06:50:23 2009 Subject: [Histonet] re: Oil Red O Message-ID: <4ADC2841.BDAE.008B.1@groupwise.upei.ca> For my Oil Red O, I prepare a stock solution of 0.3g of Oil Red O and 100ml of Isopropyl alcohol. For my working solution, I use 6ml of stock Oil Red O and 4ml distilled H2O, let that sit for 10min then filter (stable 1-2hrs). My procedure is: Wash sections well in H2O Quick dip in 60% isopropyl alcohol Place in working solution for 10-15min Differentiate in 60% isopropyl alcohol to clear background Wash in H2O Counterstain nuclei in Mayer's 3min Blue in tap H2o Mount in glycerin jelly Good Luck! Kathy I?m looking for a protocol for Oil Red O that does not use propylene glycol to make the Oil Red O. Does anyone have a protocol that I could use? Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 From pruegg <@t> ihctech.net Mon Oct 19 07:57:01 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 19 07:57:42 2009 Subject: [Histonet] looking for CD31 for rat tissues In-Reply-To: References: Message-ID: Melanie, I am very interested in your CD31 protocol, does it work on rat tissue, do you have to fix in zinc fixative? Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melanie Black Sent: Monday, October 19, 2009 12:29 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Rabbit-on-Rodent HRP polymer Hi Jen In reply to your question on the Biocare Rabbit-on rodent HRP, you are correct. I include the protocol I use, the only difference is mine is Alp phos linked instead of HRP. I use rat tissue and CD 31 antibody (from Fitzgerald) that specifically requires zinc fixed tissue. For the zinc fixation, I use exactly the same formula that Gayle Callis sent out. CD 31 Alk Phos Method for Zinc fixed Tissue. Biocare Medical Kit. Dewax slides in xylene. Take slides thru alcohol to water, and into tris buffer. Incubate with Rodent Block R (RBR962H) from Biocare Medical, for 30 mins. Wash in TBS X 2. Incubate with Primary antibody for 30 mins.(1:50) - diluted in 1% BSA in PBS. Anti Rat CD 31 from Fitzgerald. Wash in TBS X 2. Incubate in Mouse-on-Rat AP-Polymer (MRT623H), Biocare Medical, for 20 mins. Wash in TBS X 2. Add a drop of NBT(DAKO - K0598), and check for colour development. (YOU WILL USE DAB) Rinse in water, dehydrate clear and mount. NB: Sections must be cut onto UNCOATED slides, and incubated on a 60 degree hot plate for 30 mins, followed by overnight incubation at 37 degrees. Hope this helps Regards Melanie. Hi All, I recently purchased a rabbit-on-rodent HRP polymer from Biocare. I will be using this on mouse tissue, using a rabbit polyclonal CD3 as my primary. I just wanted to clarify a couple things about the protocol before I try it out tomorrow and was wondering if anyone could offer some assistance. First of all, I should aske if anyone has a protocol for this? What I really would like to make sure is that I will not have to use a label after the rabbit-on-rodent secondary ab. I believe that I will just have to apply my blocking agent, avidin/biotin blocks, primary antibody, secondary anitbody, followed by chromogen, right? My blocking agent is made up of casein and some other proprietary agents in a phosphate buffer (it is non- serum) so I was told that should work fine. Does this sound correct? Thanks in advance! I haven't been able to get through to tech support all day, so hopefully you will have some answers for me! Jen Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lyonm <@t> upstate.edu Mon Oct 19 10:25:23 2009 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Mon Oct 19 10:24:56 2009 Subject: [Histonet] Serotonin antibody Message-ID: <005301ca50d0$604e6310$20eb2930$@edu> Looking for a good human serotonin antibody. All suggestions welcome. Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 From pruegg <@t> ihctech.net Mon Oct 19 11:15:51 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 19 11:16:31 2009 Subject: [Histonet] in search of cd31 for rat tissue Message-ID: <875CB317C1F2476288D3406512F8F800@Patsyoffice> Has anyone used RECA-1 (from Novus) to stain endothelial cells in rat tissue. I also heard about RDI-RTCD31-3A12 from Fitzgerald but it requires Zinc fixative and frozen sections, I would really like to use ffpe rat tissue already archived??? Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From awatanabe <@t> tgen.org Mon Oct 19 12:07:49 2009 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Mon Oct 19 12:07:58 2009 Subject: [Histonet] RE: Oil Red O Message-ID: Thank you all for the responses. We all work alike because I?ve gotten the same protocol from most of you. Thank you again. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From Rcartun <@t> harthosp.org Mon Oct 19 17:08:38 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 19 17:08:46 2009 Subject: [Histonet] SpiraBrush CX Message-ID: <4ADCAB25.7400.0077.1@harthosp.org> Is anyone receiving specimens obtained by the SpiraBrush CX cervical biopsy device? If so, how are you fixing them? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From baderbo <@t> gmail.com Mon Oct 19 17:36:54 2009 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Mon Oct 19 17:36:58 2009 Subject: [Histonet] need help in selecting blades for Microtome Leitz1512 and good place for service Message-ID: <3c7e700910191536o6037189dle607c7f8188ea349@mail.gmail.com> Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed paraffin embedded (FFPE) tissue sections. We need your advice. We have a used Leitz 1512 microtome. ------- perhaps it needs service, can you recommend a good and reasonable place. --------what will be a good disposable blades to buy for cutting FFPE tissues sections (mostly soft tissues like colon, stomach, skin, cancer tissues etc). --------this instrument needs a blade holder, what would you recommend, Leitz does not sell blade holders for this instrument anymore. --------any good technique for cutting tissues for a person who is not expert. --------good place to buy formalin fixed tissue blocks for research use. Thanks a million. Bader e-mail address: baderbo@gmail.com From tifei <@t> foxmail.com Mon Oct 19 19:11:24 2009 From: tifei <@t> foxmail.com (TF) Date: Mon Oct 19 19:13:50 2009 Subject: [Histonet] in search of cd31 for rat tissue References: <875CB317C1F2476288D3406512F8F800@Patsyoffice> Message-ID: <200910200811234825736@foxmail.com> bmV2ZXIgc3VjY2Vzc2Z1bCBvbmNlIHUgZml4ZWQgdGhlIHRpc3N1ZSB3aXRoIFBGQS4uLmV2ZW4g bGlnaHRseQ0KDQpidXQgdSBjYW4gYWx3YXlzIGJlIHBvc2l0aXZlIG9uIGZyb3plbiAmIHppbmMg Zml4ZWQNCg0KDQoyMDA5LTEwLTIwIA0KDQoNCg0KVEYgDQoNCg0KDQq3orz+yMujuiBQYXRzeSBS dWVnZyANCreiy83Ksbzko7ogMjAwOS0xMC0yMCAgMDA6MjM6MjUgDQrK1bz+yMujuiAnSGlzdG9u ZXQnIA0Ks63LzaO6IA0K1vfM4qO6IFtIaXN0b25ldF0gaW4gc2VhcmNoIG9mIGNkMzEgZm9yIHJh dCB0aXNzdWUgDQogDQpIYXMgYW55b25lIHVzZWQgUkVDQS0xIChmcm9tIE5vdnVzKSB0byBzdGFp biBlbmRvdGhlbGlhbCBjZWxscyBpbiByYXQNCnRpc3N1ZS4gIEkgYWxzbyBoZWFyZCBhYm91dCBS REktUlRDRDMxLTNBMTIgZnJvbSBGaXR6Z2VyYWxkIGJ1dCBpdCByZXF1aXJlcw0KWmluYyBmaXhh dGl2ZSBhbmQgZnJvemVuIHNlY3Rpb25zLCBJIHdvdWxkIHJlYWxseSBsaWtlIHRvIHVzZSBmZnBl IHJhdA0KdGlzc3VlIGFscmVhZHkgYXJjaGl2ZWQ/Pz8NCg0KUmVnYXJkcywNCg0KUGF0c3kNCg0K UGF0c3kgUnVlZ2csIEhUKEFTQ1ApUUlIQw0KSUhDdGVjaCwgTExDDQpGaXR6c2ltbW9ucyBCaW9T Y2llbmNlIFBhcmsNCjEyNjM1IE1vbnR2aWV3IEJsdmQuIFN1aXRlIDIxNQ0KQXVyb3JhLCBDTyA4 MDAxMA0KUC03MjAtODU5LTQwNjANCkYtNzIwLTg1OS00MTEwDQp3ayBlbWFpbCBwcnVlZ2dAaWhj dGVjaC5uZXQNCndlYiBzaXRlIHd3dy5paGN0ZWNoLm5ldA0KDQpUaGlzIGVtYWlsIGlzIGNvbmZp ZGVudGlhbCBhbmQgaW50ZW5kZWQgc29sZWx5IGZvciB0aGUgdXNlIG9mIHRoZSBQZXJzb24ocykN CigndGhlIGludGVuZGVkIHJlY2lwaWVudCcpIHRvIHdob20gaXQgd2FzIGFkZHJlc3NlZC4gQW55 IHZpZXdzIG9yIG9waW5pb25zDQpwcmVzZW50ZWQgYXJlIHNvbGVseSB0aG9zZSBvZiB0aGUgYXV0 aG9yLiBJdCBtYXkgY29udGFpbiBpbmZvcm1hdGlvbiB0aGF0IGlzDQpwcml2aWxlZ2VkICYgY29u ZmlkZW50aWFsIHdpdGhpbiB0aGUgbWVhbmluZyBvZiBhcHBsaWNhYmxlIGxhdy4gQWNjb3JkaW5n bHkNCmFueSBkaXNzZW1pbmF0aW9uLCBkaXN0cmlidXRpb24sIGNvcHlpbmcsIG9yIG90aGVyIHVz ZSBvZiB0aGlzIG1lc3NhZ2UsIG9yDQphbnkgb2YgaXRzIGNvbnRlbnRzLCBieSBhbnkgcGVyc29u IG90aGVyIHRoYW4gdGhlIGludGVuZGVkIHJlY2lwaWVudCBtYXkNCmNvbnN0aXR1dGUgYSBicmVh Y2ggb2YgY2l2aWwgb3IgY3JpbWluYWwgbGF3IGFuZCBpcyBzdHJpY3RseSBwcm9oaWJpdGVkLiBJ Zg0KeW91IGFyZSBOT1QgdGhlIGludGVuZGVkIHJlY2lwaWVudCBwbGVhc2UgY29udGFjdCB0aGUg c2VuZGVyIGFuZCBkaXNwb3NlIG9mDQp0aGlzIGUtbWFpbCBhcyBzb29uIGFzIHBvc3NpYmxlLg0K DQpfX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9u ZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6 Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K From nyilmaz <@t> mersin.edu.tr Tue Oct 20 04:25:12 2009 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Tue Oct 20 04:25:23 2009 Subject: [Histonet] novocastra eNOS antibody on rat tissues??? Message-ID: <000901ca5167$3bfb3280$e201640a@nejat1> Dear All, We're planning to study FFPE rat aorta specimens with anti endothelial nitric oxide synthase (NOS-3) antibody. We want to perform that with Novocastra brand anti-eNOS antibody (Product Code: NCL-NOS-3). According to datasheet the antibody works on human tissues only, other species not tested yet. Does anybody have any experince with this antibody on different species other than human? Thanks in advance. Necat Yilmaz MD PhD University of Mersin School of Medicine From kmerriam2003 <@t> yahoo.com Tue Oct 20 08:22:12 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Oct 20 08:22:16 2009 Subject: [Histonet] harvest DRG from dogs Message-ID: <888635.10861.qm@web50306.mail.re2.yahoo.com> Hi Everyone, This is slightly off-topic, but does anyone know of a textbook or website that will give information on how to harvest DRG?(dorsal root ganglion) from dogs? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Arlene.Armandi <@t> cshs.org Tue Oct 20 09:14:38 2009 From: Arlene.Armandi <@t> cshs.org (Armandi, Arlene) Date: Tue Oct 20 09:14:44 2009 Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service In-Reply-To: <3c7e700910191536o6037189dle607c7f8188ea349@mail.gmail.com> Message-ID: Hi Bader, I've been a Histotechnologist for 30 years, and the Leitz 1512 is the best microtome I have ever used. (And I've used a lot)I can easily cut 1-2 micron sections of any tissue, including bone. I don't know where you are located, but if you are anywhere near Los Angeles, "Fritz Instruments" serviced my three 1512s for years and always did an excellent job. They are great with older microtomes. As for disposable blades, the Tissue-Tek Accu-Edge blades are the best on the market. If you are looking for the knife holder that is part of the original instrument, try IMEB instruments, they carry used 1512s and probably have some spare parts. Ph# 800-543-8496. or imebinc.com If you are looking for the blade holder that fits into the 1512's knife holder, that is Tissue-Tek #4687. IMEB also carries that, as does EMS ( Electron Microscopy Sciences) Cat# 63048-10 800-523-58874 A couple of hints/suggestions for using the 1512... -When you trim your blocks, don't "rock" the handwheel, make complete rotations. -Oil the unit weekly (if you look under the top lid you should see that the oil ports are marked.) - Lubricate the Teflon plate (black plate under the knife holder) at least weekly (more often if heavy use). Use microtome grease (not oil) for this. - I have always had to remove the knife guards on the unit to allow for using a disposable blade holder. The units were originally made to be used with the old reusable "hard knives", thus they were made with the knife guards built in. Hope some of this helps. If you have any other questions about the 1512, please feel free to call or e-mail me. Arlene Armandi, HTL, HT(ASCP) Cedars-Sinai Medical Center Los Angeles, CA 90048 310-423-4756 arlene.armandi@cshs.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bader Siddiki Sent: Monday, October 19, 2009 3:37 PM To: Histonet Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed paraffin embedded (FFPE) tissue sections. We need your advice. We have a used Leitz 1512 microtome. ------- perhaps it needs service, can you recommend a good and reasonable place. --------what will be a good disposable blades to buy for cutting FFPE tissues sections (mostly soft tissues like colon, stomach, skin, cancer tissues etc). --------this instrument needs a blade holder, what would you recommend, Leitz does not sell blade holders for this instrument anymore. --------any good technique for cutting tissues for a person who is not expert. --------good place to buy formalin fixed tissue blocks for research use. Thanks a million. Bader e-mail address: baderbo@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From JWeems <@t> sjha.org Tue Oct 20 09:32:13 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 20 09:32:22 2009 Subject: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service In-Reply-To: References: <3c7e700910191536o6037189dle607c7f8188ea349@mail.gmail.com> Message-ID: And if you want to speak to someone who trained in the Leitz factory, Klaus Dern is in GA - Microprecision Instruments Corp. 706-635-8840. He can probably build you one!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Armandi, Arlene Sent: Tuesday, October 20, 2009 10:15 To: Bader Siddiki; Histonet Subject: RE: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service Hi Bader, I've been a Histotechnologist for 30 years, and the Leitz 1512 is the best microtome I have ever used. (And I've used a lot)I can easily cut 1-2 micron sections of any tissue, including bone. I don't know where you are located, but if you are anywhere near Los Angeles, "Fritz Instruments" serviced my three 1512s for years and always did an excellent job. They are great with older microtomes. As for disposable blades, the Tissue-Tek Accu-Edge blades are the best on the market. If you are looking for the knife holder that is part of the original instrument, try IMEB instruments, they carry used 1512s and probably have some spare parts. Ph# 800-543-8496. or imebinc.com If you are looking for the blade holder that fits into the 1512's knife holder, that is Tissue-Tek #4687. IMEB also carries that, as does EMS ( Electron Microscopy Sciences) Cat# 63048-10 800-523-58874 A couple of hints/suggestions for using the 1512... -When you trim your blocks, don't "rock" the handwheel, make complete rotations. -Oil the unit weekly (if you look under the top lid you should see that the oil ports are marked.) - Lubricate the Teflon plate (black plate under the knife holder) at least weekly (more often if heavy use). Use microtome grease (not oil) for this. - I have always had to remove the knife guards on the unit to allow for using a disposable blade holder. The units were originally made to be used with the old reusable "hard knives", thus they were made with the knife guards built in. Hope some of this helps. If you have any other questions about the 1512, please feel free to call or e-mail me. Arlene Armandi, HTL, HT(ASCP) Cedars-Sinai Medical Center Los Angeles, CA 90048 310-423-4756 arlene.armandi@cshs.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bader Siddiki Sent: Monday, October 19, 2009 3:37 PM To: Histonet Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed paraffin embedded (FFPE) tissue sections. We need your advice. We have a used Leitz 1512 microtome. ------- perhaps it needs service, can you recommend a good and reasonable place. --------what will be a good disposable blades to buy for cutting FFPE tissues sections (mostly soft tissues like colon, stomach, skin, cancer tissues etc). --------this instrument needs a blade holder, what would you recommend, Leitz does not sell blade holders for this instrument anymore. --------any good technique for cutting tissues for a person who is not expert. --------good place to buy formalin fixed tissue blocks for research use. Thanks a million. Bader e-mail address: baderbo@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Janet.Bonner <@t> FLHOSP.ORG Tue Oct 20 09:41:36 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Oct 20 09:45:32 2009 Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service References: <3c7e700910191536o6037189dle607c7f8188ea349@mail.gmail.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2B6C@fhosxchmb006.ADVENTISTCORP.NET> Well, I can take a stab at your last question - call a large Hospital Pathology Department. We keep ten years and each year we have to disgard a year. We pay a disposal company, but they may be willing to negotiate with you. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bader Siddiki Sent: Mon 10/19/2009 6:36 PM To: Histonet Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed paraffin embedded (FFPE) tissue sections. We need your advice. We have a used Leitz 1512 microtome. ------- perhaps it needs service, can you recommend a good and reasonable place. --------what will be a good disposable blades to buy for cutting FFPE tissues sections (mostly soft tissues like colon, stomach, skin, cancer tissues etc). --------this instrument needs a blade holder, what would you recommend, Leitz does not sell blade holders for this instrument anymore. --------any good technique for cutting tissues for a person who is not expert. --------good place to buy formalin fixed tissue blocks for research use. Thanks a million. Bader e-mail address: baderbo@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From pedro.louro <@t> spcorp.com Tue Oct 20 09:49:42 2009 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Tue Oct 20 09:49:54 2009 Subject: [Histonet] NJSH 2009 Fall Meeting and Member Appreciation Day Message-ID: Reminder............ ________________________________________________________________________ ________________________________________________ NJSH 2009 Fall Meeting and Member Appreciation Day Monday, November 2nd Location: New Jersey Hospital Association & Conference Center Address: 760 Alexander Road Princeton, NJ 08543 Phone: 609-275-4000 Meeting Schedule: 8:00-9:00 Registration and Continental Breakfast 9:00-12:15 AM Session 12:15-1:15 LUNCH 1:15-4:30 PM Session ________________________________________________________________________ __________________________________________________ ROOM 1 AM SESSION: "Review of Basic Theories of Histotechnology" Linda Foster-Brown, MS, HTL(ASCP) AstraZeneca Pharmaceuticals, Wilmington, Delaware This workshop will serve as a review for working Histologists to help them deal effectively with the daily operations of the histology laboratory. It will also serve as overview for the registry eligible candidates preparing for the HT/HTL registry exam. Topics that will be covered include histological theories on the following: lab safety, fixation, processing, microtomy, and staining, as well as registry eligibility requirements. PM SESSION: "Troubleshooting Automated Special Stains" Debra Cobb, MBA, HT (ASCP) Manager of R&D, Artisan In today's world of automation in the Histology Laboratory, troubleshooting special stains and adjusting the workflow has become more complicated. This workshop will address the common issues such as: Background staining on slides, Debris on Slides, Uneven staining, Stain too dark/too light, Workflow, Turn around Time, and will address the statement "This doesn't happen when I stain manually!" This speaker is sponsored by DAKO. ROOM 2 AM SESSION: "Where Do I Find That Antibody & What Do I Do With It Once I Have It?" Christine 'Charlie' Dorner, HT(ASCP) Dako Applications Specialist - Trainer In the ever changing world of IHC, there are new antibodies and clones being developed every day by many different sources. This seminar will first cover the various ways of locating and obtaining antibodies and different formats that they are available in. Additional discussion will center on choosing Monoclonal or polyclonal antibodies, picking the right detection kit, and use of additional blocking or enhancements. Participants will then learn what to do with new antibodies once they are in their lab including optimization, troubleshooting, deciding on proper control and testing materials and record keeping. Upon completion of this course, the participants will have ideas to aide in the development and structure of an antibody development program for their facility. This speaker is sponsored by DAKO. PM SESSION: "Ethics in Imaging" Anne Lewin, BS, HT(ASCP)QIHC Bristol-Myers Squibb, Princeton, NJ A growing concern in the scientific community is the ease in which image manipulation can occur. Something that used to be done in a darkroom is now processed digitally, and there are many powerful image editing programs available. Data can be highlighted or erased with ease, and in the case of histology samples, colors can be changed or created in an image. A potential pit-fall is that an image can be altered in an unethical manner without the person realizing it. Many times an image will be "cleaned up" and important data will be lost or altered. In the case of immunohistochemical analysis, when cell number counts are used for statistical significance, attempting to get a nicer picture could skew the raw data points by missing weakly positive cells or by counting background staining as positive. This workshop will look into the history of image manipulation and the credibility issues journalists have faced since the early days of photography. We will then move onto the many options for digital image capture. Most scientific journals have very few guidelines as to what sorts of digital manipulations are acceptable when preparing a manuscript for submission. Examples of image adjustments will be presented, and potential digital imaging guidelines will be discussed. ________________________________________________________________________ _______________________________________________ DIRECTIONS: An extensive list of directions can be found on the NJHA Conference Center Website: www.njha.com/conferencecntr/html/mc.directions.aspx Please park in the rear of the building! This meeting is brought to you in part by: DAKO North America, Inc. NJSH 2009 Fall Meeting Registration Form Mail in Registration Deadline: October 20th Please register early as classroom space is limited! Walk in registrations will be accepted if space is available for an additional $20 fee. ________________________________________________________________________ __________________________________________________ NAME: ______________________________________________ ADDRESS: ___________________________________________ ___________________________________________ PHONE NUMBER: ____________________________________ Please provide your E-MAIL address to receive confirmation of your registration: ________________________________________________________________________ __ Please put an X in the room you would like to attend in each session. SESSIONS ROOM 1 ROOM 2 AM PM Cost of Registration: $75, Students $35 Registration Fee includes attendance to an AM and PM session, Continental Breakfast, and a generous Buffet Lunch! Students will be asked to present a valid student ID at the registration table. Please make your check payable to NJSH and mail with your form to: Michele French 1711 Diamond Street Sellersville, PA 18960 The NJSH would like to express our sincere thanks to DAKO for providing funding for the Continental Breakfast and for sponsoring two of our speakers. Representatives will be available throughout the day to answer your questions about their products and services. Be sure to let them know that you appreciate their support! Pedro Louro, MBA Vice President and Co-Chair Membership Committee New Jersey Society for Histotechnology (NJSH) Ph. 908-473-4501 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From algranth <@t> email.arizona.edu Tue Oct 20 10:08:58 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Oct 20 10:09:06 2009 Subject: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service In-Reply-To: References: <3c7e700910191536o6037189dle607c7f8188ea349@mail.gmail.com> Message-ID: <3ABA117E-5EA9-42E8-AC46-4132D9909A69@email.arizona.edu> I agree. Klaus is probably your best bet. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Oct 20, 2009, at 7:32 AM, Weems, Joyce wrote: > And if you want to speak to someone who trained in the Leitz factory, > Klaus Dern is in GA - Microprecision Instruments Corp. 706-635-8840. > He > can probably build you one!! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Armandi, > Arlene > Sent: Tuesday, October 20, 2009 10:15 > To: Bader Siddiki; Histonet > Subject: RE: [Histonet] need help in selecting blades for > MicrotomeLeitz1512and good place for service > > Hi Bader, > I've been a Histotechnologist for 30 years, and the Leitz 1512 is the > best microtome I have ever used. (And I've used a lot)I can easily cut > 1-2 micron sections of any tissue, including bone. > I don't know where you are located, but if you are anywhere near Los > Angeles, "Fritz Instruments" serviced my three 1512s for years and > always did an excellent job. They are great with older microtomes. > As for disposable blades, the Tissue-Tek Accu-Edge blades are the best > on the market. > If you are looking for the knife holder that is part of the original > instrument, try IMEB instruments, they carry used 1512s and probably > have some spare parts. Ph# 800-543-8496. or imebinc.com If you are > looking for the blade holder that fits into the 1512's knife holder, > that is Tissue-Tek #4687. IMEB also carries that, as does EMS > ( Electron > Microscopy Sciences) Cat# 63048-10 800-523-58874 A couple of > hints/suggestions for using the 1512... > -When you trim your blocks, don't "rock" the handwheel, make complete > rotations. > -Oil the unit weekly (if you look under the top lid you should see > that > the oil ports are marked.) > - Lubricate the Teflon plate (black plate under the knife holder) at > least weekly (more often if heavy use). Use microtome grease (not oil) > for this. > - I have always had to remove the knife guards on the unit to allow > for > using a disposable blade holder. The units were originally made to be > used with the old reusable "hard knives", thus they were made with the > knife guards built in. > Hope some of this helps. If you have any other questions about the > 1512, please feel free to call or e-mail me. > > > > Arlene Armandi, HTL, HT(ASCP) > Cedars-Sinai Medical Center > Los Angeles, CA 90048 > 310-423-4756 > arlene.armandi@cshs.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bader > Siddiki > Sent: Monday, October 19, 2009 3:37 PM > To: Histonet > Subject: [Histonet] need help in selecting blades for Microtome > Leitz1512and good place for service > > Hello Histonets > I need some help and advice from you experts. > We are new in cutting formalin fixed paraffin embedded (FFPE) tissue > sections. > We need your advice. > We have a used Leitz 1512 microtome. > ------- perhaps it needs service, can you recommend a good and > reasonable > place. > --------what will be a good disposable blades to buy for cutting FFPE > tissues sections (mostly soft tissues like colon, stomach, skin, > cancer > tissues etc). > --------this instrument needs a blade holder, what would you > recommend, > Leitz does not sell blade holders for this instrument anymore. > --------any good technique for cutting tissues for a person who is not > expert. > --------good place to buy formalin fixed tissue blocks for research > use. > > Thanks a million. > Bader > > e-mail address: baderbo@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > IMPORTANT WARNING: This message is intended for the use of the person > or entity to which it is addressed and may contain information that is > privileged and confidential, the disclosure of which is governed by > applicable law. If the reader of this message is not the intended > recipient, or the employee or agent responsible for delivering it to > the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this information is STRICTLY PROHIBITED. > > If you have received this message in error, please notify us > immediately > by calling (310) 423-6428 and destroy the related message. Thank You > for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Donald.Gose <@t> lovelace.com Tue Oct 20 10:25:31 2009 From: Donald.Gose <@t> lovelace.com (Donald Gose) Date: Tue Oct 20 10:25:40 2009 Subject: [Histonet] PCP Controls for BAL Cytology Thin Preps Message-ID: I have a request from a pathologist to use a Cytology control for PCP as opposed to a commercially prepared tissue control for the GMS stain. Has anyone heard of this before and have you found a source for a Cytology PCP control? From NHeath <@t> Lifespan.org Tue Oct 20 10:36:23 2009 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Tue Oct 20 10:36:28 2009 Subject: [Histonet] autopsy Message-ID: <130E8991F210424096EFC6F42EA33B2405328E52@LSCOEXCH1.lsmaster.lifespan.org> what would be the contributing factors to cause autopsy tissue to "be fried" or dry and brittle when sectioning?? From SSmith <@t> gsmc.org Tue Oct 20 10:43:49 2009 From: SSmith <@t> gsmc.org (Sheila Smith) Date: Tue Oct 20 10:43:51 2009 Subject: [Histonet] Modified Mowry's Colloidal Iron Stain Method Message-ID: <1DA1D448F283CA43813F2347A8DE1E09015F2D62F5@GSMCMX.corp.gsmc.org> Could someone provide the Modified Mowry's Colloid Iron stain technique? Thank you, Sheila Smith Good Shepherd Medical Center Histology Supervisor Phone: 903-315-2410 Fax: 903-315-2044 ssmith@gsmc.org From rjbuesa <@t> yahoo.com Tue Oct 20 10:51:57 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 20 10:52:47 2009 Subject: [Histonet] autopsy In-Reply-To: <130E8991F210424096EFC6F42EA33B2405328E52@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <702637.50814.qm@web65715.mail.ac4.yahoo.com> Probably because it took too long for the tissues to be placed in NBF after the death occurred or after they were taken from the cadaver (probably left in the air). Ren? J. --- On Tue, 10/20/09, Heath, Nancy L. wrote: From: Heath, Nancy L. Subject: [Histonet] autopsy To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 20, 2009, 11:36 AM what would be the contributing factors to cause autopsy tissue to "be fried" or dry and brittle when sectioning?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Tue Oct 20 10:59:31 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Oct 20 10:59:44 2009 Subject: [Histonet] Modified Mowry's Colloidal Iron Stain Method In-Reply-To: <1DA1D448F283CA43813F2347A8DE1E09015F2D62F5@GSMCMX.corp.gsmc.org> References: <1DA1D448F283CA43813F2347A8DE1E09015F2D62F5@GSMCMX.corp.gsmc.org> Message-ID: http://stainsfile.info/StainsFile/stain/carbohydrate/colloidaliron-mowry.htm ----- Original Message ----- From: "Sheila Smith" To: Sent: Tuesday, October 20, 2009 8:43 AM Subject: [Histonet] Modified Mowry's Colloidal Iron Stain Method Could someone provide the Modified Mowry's Colloid Iron stain technique? Thank you, Sheila Smith Good Shepherd Medical Center Histology Supervisor Phone: 903-315-2410 Fax: 903-315-2044 ssmith@gsmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Oct 20 13:21:47 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Oct 20 13:21:51 2009 Subject: [Histonet] Antigen retrieval question Message-ID: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tao_janet <@t> yahoo.com Tue Oct 20 13:30:55 2009 From: tao_janet <@t> yahoo.com (Ms Janet Tao) Date: Tue Oct 20 13:30:59 2009 Subject: [Histonet] Antigen retrieval question In-Reply-To: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> Message-ID: <491199.26795.qm@web57008.mail.re3.yahoo.com> Sounds like the retrieved antigen was lost rather than "reversal" Janet QIHC/HTL --- On Tue, 10/20/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 20, 2009, 11:21 AM Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com ? Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Oct 20 13:33:04 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Oct 20 13:33:18 2009 Subject: [Histonet] RE: Antigen retrieval question In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF72820A6@EXMBMCB15.ucsfmedicalcenter.org> References: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> <1AAF670737F193429070841C6B2ADD4CF72820A6@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <63EA0607835FBA4689CEA9EA8B482692026E4C6F@usctmx1141.merck.com> Perhaps reversal is not the right word...slides were left overnight in buffer w/ tween. We got very minimal faint staining compared to previous runs done in one day. Brett -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 20, 2009 2:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From janderson <@t> halozyme.com Tue Oct 20 13:39:45 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Tue Oct 20 13:40:01 2009 Subject: [Histonet] Leica Autostainer Message-ID: <5F7CC9B788911848A79BC83453A3D6530360B5D7D4@Tomlinson.hti.com> Good Afternoon Histonet. Our lab just purchased a Leica XL for routine H&E and hopefully Trichrome. I have never used this instrument before, and was hoping for some direction on where to optimally place reagents in the machine for the best (and easiest) staining runs. Thanks so much for your help. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From dwitt <@t> sebh.org Tue Oct 20 13:42:29 2009 From: dwitt <@t> sebh.org (Witt, Dan) Date: Tue Oct 20 13:42:35 2009 Subject: [Histonet] Unsubscribe from website Message-ID: I would appreciate you removing me from the Histonet website Dan Witt PA, HT (ASCP) Pathologists Assistant / Histology Coordinator St. Elizabeth's Hospital 211 S. 3 rd Street Belleville, IL 62220 234-2120 Ext. 1369 or 1192 From trathborne <@t> somerset-healthcare.com Tue Oct 20 13:48:44 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Oct 20 13:48:49 2009 Subject: [Histonet] Leica Autostainer In-Reply-To: <5F7CC9B788911848A79BC83453A3D6530360B5D7D4@Tomlinson.hti.com> Message-ID: Contact Carolyn Doan at Leica, she's an expert at setting up staining protocols. Carolyn.Doan@leica-microsystems.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer Anderson Sent: Tuesday, October 20, 2009 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Autostainer Good Afternoon Histonet. Our lab just purchased a Leica XL for routine H&E and hopefully Trichrome. I have never used this instrument before, and was hoping for some direction on where to optimally place reagents in the machine for the best (and easiest) staining runs. Thanks so much for your help. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From carrolpb <@t> umdnj.edu Tue Oct 20 14:02:10 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Oct 20 14:02:25 2009 Subject: [Histonet] Unsubscribe from website In-Reply-To: References: Message-ID: <4ADE0932.4050406@umdnj.edu> There is a link at the bottom of this and every message on this list. Click it. Scroll to the bottom. Unsubscribe yourself. Easy, right? Witt, Dan wrote: > I would appreciate you removing me from the Histonet website > > > Dan Witt PA, HT (ASCP) > > Pathologists Assistant / Histology Coordinator > > St. Elizabeth's Hospital > > 211 S. 3 rd Street > > Belleville, IL 62220 > > 234-2120 Ext. 1369 or 1192 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From rosenfeldtek <@t> hotmail.com Tue Oct 20 14:43:22 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Oct 20 14:43:27 2009 Subject: [Histonet] RE: Antigen retrieval question Message-ID: Perhaps the antigen is soluble and diffused out of the tissue into the buffer? Jerry Ricks Research Scientist University of Washington Department of Pathology histonet@lists.utsouthwestern.edu Perhaps reversal is not the right word...slides were left overnight in buffer w/ tween. We got very minimal faint staining compared to previous runs done in one day. Brett -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 20, 2009 2:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/177141665/direct/01/ From rjbuesa <@t> yahoo.com Tue Oct 20 14:47:41 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 20 14:47:46 2009 Subject: [Histonet] Antigen retrieval question In-Reply-To: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> Message-ID: <936839.34911.qm@web65701.mail.ac4.yahoo.com> Consider the following sequence: 1- the NBF corsslinked the antigens making them "more" stable, "insolubable". 2- you did HIER and that crosslinkage disappeared, making the antigen "vulnerable" or able to be dissolved 3- you left them in buffer while "vulnerable". Does not sound to you as if the antigens were "diluted", "dissolved" in the buffer causing a weaker reaction than usual? That would be my explanation, but to your original question ("Have you experienced...") my answer is no, I always proceeded with the IHC test immediately after HIER. Maybe that is why?it has not happened to me. Just a thought! Ren? J. --- On Tue, 10/20/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 20, 2009, 2:21 PM Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com ? Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Maria.Mejia <@t> ucsf.edu Tue Oct 20 17:09:51 2009 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Tue Oct 20 17:09:57 2009 Subject: [Histonet] RE: Antigen retrieval question In-Reply-To: <63EA0607835FBA4689CEA9EA8B482692026E4C6F@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> <1AAF670737F193429070841C6B2ADD4CF72820A6@EXMBMCB15.ucsfmedicalcenter.org>, <63EA0607835FBA4689CEA9EA8B482692026E4C6F@usctmx1141.merck.com> Message-ID: Brett, We mostly work & do IHC on 40um free-floating brain sections. These sections were cut from fixed brain blocks. Sometimes, not on a regular basis, but sometimes we have had to place our sections in (plain) PBS solution & stored at 4C - overnight before continuing the IHC protocol with no effect to the staining quality. Keep in mind, that this was done before the primary antibody. We have also placed our cut and unstained sections in plate wells (5ml of PBS) - again at 4C for 2 days before starting our IHC protocol. We use quite a number of different antibodies & stain using polymer. I hope this helps. Maria Mejia Histology Manager Department of Neurosurgery UCSF Sf, CA 94103 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M [brett_connolly@merck.com] Sent: Tuesday, October 20, 2009 11:33 AM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Antigen retrieval question Perhaps reversal is not the right word...slides were left overnight in buffer w/ tween. We got very minimal faint staining compared to previous runs done in one day. Brett -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 20, 2009 2:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.PALMER <@t> svhm.org.au Tue Oct 20 18:08:54 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Tue Oct 20 18:09:02 2009 Subject: [Histonet] RE: in search of cd31 for rat tissue (TF) AND novocastra eNOS antibody on rat tissues??? Message-ID: To possibly kill (or at least wound a little?) two birds with the one stone... We have used BD Transduction anti-eNOS, # 610296 successfully on FFPE rat tissue, if that is any help. It seems to label the endothelium in all vessels in the tissues we have looked at so far, as far as we can tell (eNOS being a constitutive form of NOS) and therefore may be considered a vascular marker. Cheers, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ?????? [Histonet] in search of cd31 for rat tissue Has anyone used RECA-1 (from Novus) to stain endothelial cells in rat tissue. I also heard about RDI-RTCD31-3A12 from Fitzgerald but it requires Zinc fixative and frozen sections, I would really like to use ffpe rat tissue already archived??? Regards, Patsy ------------------------------ From: "Nejat Yilmaz" Subject: [Histonet] novocastra eNOS antibody on rat tissues??? Dear All, We're planning to study FFPE rat aorta specimens with anti endothelial nitric oxide synthase (NOS-3) antibody. We want to perform that with Novocastra brand anti-eNOS antibody (Product Code: NCL-NOS-3). According to datasheet the antibody works on human tissues only, other species not tested yet. Does anybody have any experince with this antibody on different species other than human? Thanks in advance. Necat Yilmaz MD PhD University of Mersin School of Medicine Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From rcampbe <@t> frontiernet.net Tue Oct 20 18:14:50 2009 From: rcampbe <@t> frontiernet.net (Jennifer Campbell) Date: Tue Oct 20 18:15:08 2009 Subject: [Histonet] Fite control slides Message-ID: I am looking for a reputable vendor for Fite control slides. My pathologist would prefer they be "leprosy containing skin slides". I also need the vendor to provide a positvely stained slide with the procedure as well. Thank you in advance Jen Campbell From jcampbell <@t> vdxpathology.com Tue Oct 20 20:22:26 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Tue Oct 20 20:22:31 2009 Subject: [Histonet] Granzyme B and F4/80 immunostaining Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FB8@VDXSERVER01.vdxpathology.local> Hi All, I'm currently trying to work up Granzyme B and F4/80 on FFPE mouse tissue. I seemed to get a bit of non-specific staining for both and was wondering if there is any way I can eliminate some of this. For Granzyme B I am using a rabbit polyclonal from Abcam. My protocol goes like this: H202 block, citrate buffer HIER, Powerblock (casein-based, non-serum protein block), primary antibody, secondary antibody (rabbit-on rodent HRP polymer, from biocare), DAB, counterstain. My F4/80 is a rat anti-mouse primary from Serotec and the protocol is as follows: H202 block, citrate buffer HIER, Powerblock, avidin/biotin blocks, primary antibody, secondary antibody (Goat anti-rat, biotinylated, mouse-absorbed, from Biocare), HRP Label, DAB, counterstain. Is it true that if I switched to a serum block from the species in which the secondary was raised, instead of my non-serum protein block, it may help? Thank you in advance! Jennifer From Melanie.Black <@t> uct.ac.za Wed Oct 21 02:13:19 2009 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Wed Oct 21 02:15:00 2009 Subject: [Histonet] CD 31 in rats Message-ID: <639A6331-F3C7-4823-8D7C-38825F051AB8@uct.ac.za> Hi Patsy I have tried RECA-1 and not had great success. The Fitzgerald one is the best and we have researched this plenty. One option for PFA fixed rat tissue is the Abcam VWF ( ab6994). I use it 1:400 overnight incubation of primary with 10 minutes proteinase K before hand, followed by Donkey anti-Rabbit Cy 3 (fluorescent stain). Works well. Melanie. Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From sbreeden <@t> nmda.nmsu.edu Wed Oct 21 07:48:57 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Oct 21 07:49:28 2009 Subject: [Histonet] Leica Stainer Question Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BAC@nmdamailsvr.nmda.ad.nmsu.edu> I use the Leica AutoStainer XL (and love it). I need to program it for a run FROM water through the H&E part through to XYL. I have a program TO water for special stains, IHC, etc., but have not programmed it for a run when I mess up and forget to reset for the routine H&E run after I've done a run to water run. If you've already figured this out, I'd appreciate the steps - I'm not lazy, I'm just not a programmer. Can anyone help a technologically-challenged person such as myself? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Kathleen.Cormier <@t> crl.com Wed Oct 21 10:11:16 2009 From: Kathleen.Cormier <@t> crl.com (Cormier, Kathleen) Date: Wed Oct 21 10:11:45 2009 Subject: [Histonet] Beckstead ZSF FW: Zn fixative 550523 vs 552658 Message-ID: <7862ACBA8359E04FA9B0C5375A2E6F466A7191@shr-sv0014.na01.crl.com> Follow up to the Beckstead Zinc IHC fixative saga.....BD Pharmingen states that their IHC Zinc fix does contain the proper ratio/ingredients to make it identical to the Beckstead fix. See below...Thanks to everyone!! Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Experience the new www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. ________________________________ From: Sun_Min_Lee@bd.com [mailto:Sun_Min_Lee@bd.com] Sent: Tuesday, October 20, 2009 12:13 PM To: Cormier, Kathleen Subject: Zn fixative 550523 vs 552658 Hi Kathleen, Thank you for your interest in BD Biosciences Pharmingen product. Further to your inquiry, the products cat. no. 550523 (1x Zn Fixative) and cat. no. 552658 (10X Zn Fixative) are very similar (almost the same) in terms of ingredients. Both products contain the same quantity of Calcium Acetate, Zinc Acetate, and Zinc Chloride per 1x Liter. Hope this would be helpful and please let us know if you have any further question. Best Regards, Sun Min Lee, PhD Scientist Technical Service, Research Reagents and Applications BD Biosciences Tel: 877 232 8995, option 3, option 2; Fax: 408-954-2722 Email: Sun_Min_Lee@bd.com, ResearchApplications@bd.com Please complete a brief survey to provide feedback on your experience with technical service. ________________________________ ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* From ian.montgomery <@t> bio.gla.ac.uk Wed Oct 21 10:15:27 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Oct 21 10:15:53 2009 Subject: [Histonet] Paraplast Message-ID: <10A1FA34C5234A85A9792C3AD5C19D30@IBLS.GLA.AC.UK> Has anyone noticed changes in tissue fluorescence if using Paraplast, Paraplast plus or Paraplast extra? E.g. increased autofluorescence, increased or decreased signal after staining, anything really. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From Paula.Stanjeski <@t> HCAhealthcare.com Wed Oct 21 11:17:32 2009 From: Paula.Stanjeski <@t> HCAhealthcare.com (Stanjeski Paula) Date: Wed Oct 21 11:17:24 2009 Subject: [Histonet] PRN.POSITION Message-ID: <14D454E82AC4804CA378B2ABDC7A3B5D872BD7F57E@FWDCWPMSGCMS10.hca.corpad.net> Hello, We are seeking a PRN histotech for Tuesday and Thursday of each week,6:00 am to 2:30 pm. Must be a team player and be flexible to fill in other shifts when needed,other shifts would be 7:00 am to 3:30 And 8:30 am to 5:00pm Monday thru Friday only. (when other employees need time off) Possible after 5pm call for occasional late surgery cases with frozen sections.. Hospital based Pathology Lab,routine procedures. Located in Hudson, Florida approximately 35 miles North of Tampa,Florida. Interested applicants fax Resume to 727-819-2943 /attention Paula From brett_connolly <@t> merck.com Wed Oct 21 12:00:58 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Oct 21 12:01:08 2009 Subject: [Histonet] RE: Antigen retrieval question In-Reply-To: References: <63EA0607835FBA4689CEA9EA8B482692026E4C6F@usctmx1141.merck.com> Message-ID: <63EA0607835FBA4689CEA9EA8B482692026E4FCA@usctmx1141.merck.com> Thanks to all who replied to my question. It is not our usual procedure to leave slides overnight in buffer after AR.... and not one that we will ever repeat. Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Rcartun <@t> harthosp.org Wed Oct 21 12:03:03 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Oct 21 12:03:19 2009 Subject: [Histonet] Uranyl nitrate Message-ID: <4ADF0687.7400.0077.1@harthosp.org> Our Safety people are asking us to stop using uranyl nitrate which I believe is used in our Dieterle and Reticulin histochemical stains. Are there alternative staining protocols that do not use uranyl nitrate (forgive me because this is not my area of expertise)? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From MadaryJ <@t> MedImmune.com Wed Oct 21 12:08:41 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Oct 21 12:09:13 2009 Subject: [Histonet] llEICA sTAINER In-Reply-To: References: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A138E4956@MD1EV002.medimmune.com> You can just start step one from water as your first step. Step one Station "wash1" for example. We use that for frozen sections Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, October 21, 2009 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 71, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Antigen retrieval question (Connolly, Brett M) 2. Re: Antigen retrieval question (Ms Janet Tao) 3. RE: Antigen retrieval question (Connolly, Brett M) 4. Leica Autostainer (Jennifer Anderson) 5. Unsubscribe from website (Witt, Dan) 6. RE: Leica Autostainer (Rathborne, Toni) 7. Re: Unsubscribe from website (Peter Carroll) 8. RE: Antigen retrieval question (JR R) 9. Re: Antigen retrieval question (Rene J Buesa) 10. RE: Antigen retrieval question (Mejia, Maria) 11. RE: in search of cd31 for rat tissue (TF) AND novocastra eNOS antibody on rat tissues??? (PALMER Jason (SVHM)) 12. Fite control slides (Jennifer Campbell) 13. Granzyme B and F4/80 immunostaining (Jennifer Campbell) 14. CD 31 in rats (Melanie Black) 15. Leica Stainer Question (Breeden, Sara) 16. Beckstead ZSF FW: Zn fixative 550523 vs 552658 (Cormier, Kathleen) 17. Paraplast (Ian Montgomery) 18. PRN.POSITION (Stanjeski Paula) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Oct 2009 14:21:47 -0400 From: "Connolly, Brett M" Subject: [Histonet] Antigen retrieval question To: Message-ID: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 2 Date: Tue, 20 Oct 2009 11:30:55 -0700 (PDT) From: Ms Janet Tao Subject: Re: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu, Brett MConnolly Message-ID: <491199.26795.qm@web57008.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Sounds like the retrieved antigen was lost rather than "reversal" Janet QIHC/HTL --- On Tue, 10/20/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 20, 2009, 11:21 AM Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com ? Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 20 Oct 2009 14:33:04 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: Antigen retrieval question To: "Morken, Tim" , Message-ID: <63EA0607835FBA4689CEA9EA8B482692026E4C6F@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Perhaps reversal is not the right word...slides were left overnight in buffer w/ tween. We got very minimal faint staining compared to previous runs done in one day. Brett -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 20, 2009 2:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 4 Date: Tue, 20 Oct 2009 11:39:45 -0700 From: Jennifer Anderson Subject: [Histonet] Leica Autostainer To: "histonet@lists.utsouthwestern.edu" Message-ID: <5F7CC9B788911848A79BC83453A3D6530360B5D7D4@Tomlinson.hti.com> Content-Type: text/plain; charset="us-ascii" Good Afternoon Histonet. Our lab just purchased a Leica XL for routine H&E and hopefully Trichrome. I have never used this instrument before, and was hoping for some direction on where to optimally place reagents in the machine for the best (and easiest) staining runs. Thanks so much for your help. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ------------------------------ Message: 5 Date: Tue, 20 Oct 2009 13:42:29 -0500 From: "Witt, Dan" Subject: [Histonet] Unsubscribe from website To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I would appreciate you removing me from the Histonet website Dan Witt PA, HT (ASCP) Pathologists Assistant / Histology Coordinator St. Elizabeth's Hospital 211 S. 3 rd Street Belleville, IL 62220 234-2120 Ext. 1369 or 1192 ------------------------------ Message: 6 Date: Tue, 20 Oct 2009 14:48:44 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] Leica Autostainer To: "Jennifer Anderson" , Message-ID: Content-Type: text/plain; charset="utf-8" Contact Carolyn Doan at Leica, she's an expert at setting up staining protocols. Carolyn.Doan@leica-microsystems.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer Anderson Sent: Tuesday, October 20, 2009 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Autostainer Good Afternoon Histonet. Our lab just purchased a Leica XL for routine H&E and hopefully Trichrome. I have never used this instrument before, and was hoping for some direction on where to optimally place reagents in the machine for the best (and easiest) staining runs. Thanks so much for your help. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr ------------------------------ Message: 7 Date: Tue, 20 Oct 2009 15:02:10 -0400 From: Peter Carroll Subject: Re: [Histonet] Unsubscribe from website To: "Witt, Dan" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <4ADE0932.4050406@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed There is a link at the bottom of this and every message on this list. Click it. Scroll to the bottom. Unsubscribe yourself. Easy, right? Witt, Dan wrote: > I would appreciate you removing me from the Histonet website > > > Dan Witt PA, HT (ASCP) > > Pathologists Assistant / Histology Coordinator > > St. Elizabeth's Hospital > > 211 S. 3 rd Street > > Belleville, IL 62220 > > 234-2120 Ext. 1369 or 1192 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 8 Date: Tue, 20 Oct 2009 12:43:22 -0700 From: JR R Subject: [Histonet] RE: Antigen retrieval question To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Perhaps the antigen is soluble and diffused out of the tissue into the buffer? Jerry Ricks Research Scientist University of Washington Department of Pathology histonet@lists.utsouthwestern.edu Perhaps reversal is not the right word...slides were left overnight in buffer w/ tween. We got very minimal faint staining compared to previous runs done in one day. Brett -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 20, 2009 2:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/177141665/direct/01/ ------------------------------ Message: 9 Date: Tue, 20 Oct 2009 12:47:41 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu, Brett MConnolly Message-ID: <936839.34911.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Consider the following sequence: 1- the NBF corsslinked the antigens making them "more" stable, "insolubable". 2- you did HIER and that crosslinkage disappeared, making the antigen "vulnerable" or able to be dissolved 3- you left them in buffer while "vulnerable". Does not sound to you as if the antigens were "diluted", "dissolved" in the buffer causing a weaker reaction than usual? That would be my explanation, but to your original question ("Have you experienced...") my answer is no, I always proceeded with the IHC test immediately after HIER. Maybe that is why?it has not happened to me. Just a thought! Ren? J. --- On Tue, 10/20/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 20, 2009, 2:21 PM Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com ? Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 10 Date: Tue, 20 Oct 2009 15:09:51 -0700 From: "Mejia, Maria" Subject: [Histonet] RE: Antigen retrieval question To: "Connolly, Brett M" , "Morken, Tim" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Brett, We mostly work & do IHC on 40um free-floating brain sections. These sections were cut from fixed brain blocks. Sometimes, not on a regular basis, but sometimes we have had to place our sections in (plain) PBS solution & stored at 4C - overnight before continuing the IHC protocol with no effect to the staining quality. Keep in mind, that this was done before the primary antibody. We have also placed our cut and unstained sections in plate wells (5ml of PBS) - again at 4C for 2 days before starting our IHC protocol. We use quite a number of different antibodies & stain using polymer. I hope this helps. Maria Mejia Histology Manager Department of Neurosurgery UCSF Sf, CA 94103 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M [brett_connolly@merck.com] Sent: Tuesday, October 20, 2009 11:33 AM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Antigen retrieval question Perhaps reversal is not the right word...slides were left overnight in buffer w/ tween. We got very minimal faint staining compared to previous runs done in one day. Brett -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 20, 2009 2:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 21 Oct 2009 10:08:54 +1100 From: "PALMER Jason (SVHM)" Subject: [Histonet] RE: in search of cd31 for rat tissue (TF) AND novocastra eNOS antibody on rat tissues??? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" To possibly kill (or at least wound a little?) two birds with the one stone... We have used BD Transduction anti-eNOS, # 610296 successfully on FFPE rat tissue, if that is any help. It seems to label the endothelium in all vessels in the tissues we have looked at so far, as far as we can tell (eNOS being a constitutive form of NOS) and therefore may be considered a vascular marker. Cheers, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ?????? [Histonet] in search of cd31 for rat tissue Has anyone used RECA-1 (from Novus) to stain endothelial cells in rat tissue. I also heard about RDI-RTCD31-3A12 from Fitzgerald but it requires Zinc fixative and frozen sections, I would really like to use ffpe rat tissue already archived??? Regards, Patsy ------------------------------ From: "Nejat Yilmaz" Subject: [Histonet] novocastra eNOS antibody on rat tissues??? Dear All, We're planning to study FFPE rat aorta specimens with anti endothelial nitric oxide synthase (NOS-3) antibody. We want to perform that with Novocastra brand anti-eNOS antibody (Product Code: NCL-NOS-3). According to datasheet the antibody works on human tissues only, other species not tested yet. Does anybody have any experince with this antibody on different species other than human? Thanks in advance. Necat Yilmaz MD PhD University of Mersin School of Medicine Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 12 Date: Tue, 20 Oct 2009 19:14:50 -0400 From: "Jennifer Campbell" Subject: [Histonet] Fite control slides To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am looking for a reputable vendor for Fite control slides. My pathologist would prefer they be "leprosy containing skin slides". I also need the vendor to provide a positvely stained slide with the procedure as well. Thank you in advance Jen Campbell ------------------------------ Message: 13 Date: Tue, 20 Oct 2009 18:22:26 -0700 From: "Jennifer Campbell" Subject: [Histonet] Granzyme B and F4/80 immunostaining To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8181FB8@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" Hi All, I'm currently trying to work up Granzyme B and F4/80 on FFPE mouse tissue. I seemed to get a bit of non-specific staining for both and was wondering if there is any way I can eliminate some of this. For Granzyme B I am using a rabbit polyclonal from Abcam. My protocol goes like this: H202 block, citrate buffer HIER, Powerblock (casein-based, non-serum protein block), primary antibody, secondary antibody (rabbit-on rodent HRP polymer, from biocare), DAB, counterstain. My F4/80 is a rat anti-mouse primary from Serotec and the protocol is as follows: H202 block, citrate buffer HIER, Powerblock, avidin/biotin blocks, primary antibody, secondary antibody (Goat anti-rat, biotinylated, mouse-absorbed, from Biocare), HRP Label, DAB, counterstain. Is it true that if I switched to a serum block from the species in which the secondary was raised, instead of my non-serum protein block, it may help? Thank you in advance! Jennifer ------------------------------ Message: 14 Date: Wed, 21 Oct 2009 09:13:19 +0200 From: Melanie Black Subject: [Histonet] CD 31 in rats To: histonet@lists.utsouthwestern.edu Message-ID: <639A6331-F3C7-4823-8D7C-38825F051AB8@uct.ac.za> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi Patsy I have tried RECA-1 and not had great success. The Fitzgerald one is the best and we have researched this plenty. One option for PFA fixed rat tissue is the Abcam VWF ( ab6994). I use it 1:400 overnight incubation of primary with 10 minutes proteinase K before hand, followed by Donkey anti-Rabbit Cy 3 (fluorescent stain). Works well. Melanie. Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. ------------------------------ Message: 15 Date: Wed, 21 Oct 2009 06:48:57 -0600 From: "Breeden, Sara" Subject: [Histonet] Leica Stainer Question To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BAC@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I use the Leica AutoStainer XL (and love it). I need to program it for a run FROM water through the H&E part through to XYL. I have a program TO water for special stains, IHC, etc., but have not programmed it for a run when I mess up and forget to reset for the routine H&E run after I've done a run to water run. If you've already figured this out, I'd appreciate the steps - I'm not lazy, I'm just not a programmer. Can anyone help a technologically-challenged person such as myself? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 16 Date: Wed, 21 Oct 2009 11:11:16 -0400 From: "Cormier, Kathleen" Subject: [Histonet] Beckstead ZSF FW: Zn fixative 550523 vs 552658 To: Message-ID: <7862ACBA8359E04FA9B0C5375A2E6F466A7191@shr-sv0014.na01.crl.com> Content-Type: text/plain; charset="us-ascii" Follow up to the Beckstead Zinc IHC fixative saga.....BD Pharmingen states that their IHC Zinc fix does contain the proper ratio/ingredients to make it identical to the Beckstead fix. See below...Thanks to everyone!! Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Experience the new www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. ________________________________ From: Sun_Min_Lee@bd.com [mailto:Sun_Min_Lee@bd.com] Sent: Tuesday, October 20, 2009 12:13 PM To: Cormier, Kathleen Subject: Zn fixative 550523 vs 552658 Hi Kathleen, Thank you for your interest in BD Biosciences Pharmingen product. Further to your inquiry, the products cat. no. 550523 (1x Zn Fixative) and cat. no. 552658 (10X Zn Fixative) are very similar (almost the same) in terms of ingredients. Both products contain the same quantity of Calcium Acetate, Zinc Acetate, and Zinc Chloride per 1x Liter. Hope this would be helpful and please let us know if you have any further question. Best Regards, Sun Min Lee, PhD Scientist Technical Service, Research Reagents and Applications BD Biosciences Tel: 877 232 8995, option 3, option 2; Fax: 408-954-2722 Email: Sun_Min_Lee@bd.com, ResearchApplications@bd.com Please complete a brief survey to provide feedback on your experience with technical service. ________________________________ ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* ------------------------------ Message: 17 Date: Wed, 21 Oct 2009 16:15:27 +0100 From: "Ian Montgomery" Subject: [Histonet] Paraplast To: Message-ID: <10A1FA34C5234A85A9792C3AD5C19D30@IBLS.GLA.AC.UK> Content-Type: text/plain; charset="us-ascii" Has anyone noticed changes in tissue fluorescence if using Paraplast, Paraplast plus or Paraplast extra? E.g. increased autofluorescence, increased or decreased signal after staining, anything really. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ------------------------------ Message: 18 Date: Wed, 21 Oct 2009 11:17:32 -0500 From: Stanjeski Paula Subject: [Histonet] PRN.POSITION To: "histonet@lists.utsouthwestern.edu" Message-ID: <14D454E82AC4804CA378B2ABDC7A3B5D872BD7F57E@FWDCWPMSGCMS10.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Hello, We are seeking a PRN histotech for Tuesday and Thursday of each week,6:00 am to 2:30 pm. Must be a team player and be flexible to fill in other shifts when needed,other shifts would be 7:00 am to 3:30 And 8:30 am to 5:00pm Monday thru Friday only. (when other employees need time off) Possible after 5pm call for occasional late surgery cases with frozen sections.. Hospital based Pathology Lab,routine procedures. Located in Hudson, Florida approximately 35 miles North of Tampa,Florida. Interested applicants fax Resume to 727-819-2943 /attention Paula ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 71, Issue 21 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From eddessa <@t> emory.edu Wed Oct 14 12:31:24 2009 From: eddessa <@t> emory.edu (Dessasau III, Evan) Date: Wed Oct 21 12:12:57 2009 Subject: [Histonet] Clear Paraffin? (Monfils, Paul) Message-ID: <0C64D370C6510C4EA03708F438198DE2015F26A14DB2@EXCHANGE20.Enterprise.emory.net> ....IMEB, INC. had a clear paraffin at the NSH meeting in birmingham. They are at www.IMEBINC.com E-van ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, October 14, 2009 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 71, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. image pro plus (Sarka Lhotak) 2. price for plastic embedding (Nancy W. Troiano) 3. Clear Paraffin? (Monfils, Paul) 4. Transporting Formalin (Feher, Stephen) 5. New Lead Tech/Assistant Manager position available in NJ (Brian- Prometheus) 6. how to fix EGFP for proteomics (Lilja Thoenes) 7. Pathos Tissue Processor vs Tissue Xpress (Kiranjit Grewal) 8. hematoxylin and nova red staining (del phillips) 9. looking for calibration tools for Optimax plus stainer (Leroy Brown) 10. Re: looking for calibration tools for Optimax plus stainer (Patti Loykasek) 11. Isotype background (Adam .) 12. RE: Automated H&E stainers (Cathy) 13. RE: Transporting Formalin (DELIA GARCIA) 14. lid gasket for processor (Diana McCaig) 15. pkh (Melissa Mazan) 16. RE: lid gasket for processor (Rathborne, Toni) 17. Fw: Pathos Tissue Processor vs Tissue Xpress (Rene J Buesa) 18. Re: Isotype background (Merced M Leiker) 19. RE: Fw: Pathos Tissue Processor vs Tissue Xpress (Rene J Buesa) 20. HerCep (kkwaa@bidmc.harvard.edu) 21. unsubscribe, please (susan bryant) 22. RELIA Histology Job Alert Histotechnologist needed in Atlanta (Pam Barker) 23. Re: unsubscribe, please (Emily Sours) 24. Re: unsubscribe, please (Peter Carroll) ---------------------------------------------------------------------- Message: 1 Date: Tue, 13 Oct 2009 13:30:14 -0400 From: "Sarka Lhotak" Subject: [Histonet] image pro plus To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain;charset=utf-8 Hi Hatem, to separate RGB colours, go to Process/ Colour channel/ Extract. This will give you each channel in B&W, i.e. each pixel with intensity from 0 to 255 for Red, Blue and Green component of your image. There must be many ways how to do intensity. One that would be possible is: go to Process/ Pseudocolour/ Select number of divisions (bins). Intensities will then be divided into bins, say 0 to 10, 11 to 20, 21 to 30 etc. all the way to 255. And then look in areas, it will give you number of pixels for each range of intensities you selected in Divisions. Hope it helps, Sarka Lhotak, PhD McMaster University, Hamilton ------------------------------ Message: 2 Date: Tue, 13 Oct 2009 15:26:34 -0400 From: "Nancy W. Troiano" Subject: [Histonet] price for plastic embedding To: histonet@lists.utsouthwestern.edu Message-ID: <5.2.1.1.2.20091013152448.0145d820@email.med.yale.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Our lab prices for plastic embedding are dependent on many variables, e.g. size of bone tissue, implant/nonimplant, services required (such as embed only, embed, cut, etc.). You can contact me for pricing for work done for outside institutions. ------------------------------ Message: 3 Date: Tue, 13 Oct 2009 15:58:46 -0400 From: "Monfils, Paul" Subject: [Histonet] Clear Paraffin? To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CEF@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" I work in a core histology facility where I receive all kinds of specimens and embedded blocks from many different sources. Occasionally I receive blocks that are embedded in a clear (transparent, not white) embedding wax. Does anyone know what this product is? ------------------------------ Message: 4 Date: Tue, 13 Oct 2009 16:23:10 -0400 From: "Feher, Stephen" Subject: [Histonet] Transporting Formalin To: Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB207F@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" Hi gang, A question came up in a committee meeting today surrounding how other institutions are transporting quantities of 10% NBF from a main facility to outlying hospitals and outpatient clinics. The quantities in question ranged from 5 - 20 gallons of formalin in sealed carboy type containers. Does a main hospital or lab distribute the formalin to outlying facilities or are outlying facilities having to order direct from a supplier and bill the main facility? Some places just order it and transport via courier. This may be restricted due to various State EPA rules. How are you all doing this? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org ------------------------------ Message: 5 Date: Tue, 13 Oct 2009 16:52:32 -0400 From: "Brian- Prometheus" Subject: [Histonet] New Lead Tech/Assistant Manager position available in NJ To: Message-ID: <001901ca4c47$15e77600$41b66200$@com> Content-Type: text/plain; charset="us-ascii" New Opening with a lab in Paramus Day shift 5am to 1:30pm or 6:00a to 2:30pm Stresses quality over quantity Prefers ASCP certification and NY state licensure Someone who can multitask, detail oriented IHC experience a plus Salary will commensurate upon experience Minimum 10 yrs exp Lab is growing, consistently acquiring new clients. Been in business about a year Please let me know if you may be interested! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog ------------------------------ Message: 6 Date: Fri, 9 Oct 2009 16:01:23 +0100 From: Lilja Thoenes Subject: [Histonet] how to fix EGFP for proteomics To: "histonet@lists.utsouthwestern.edu" Message-ID: <54E3312540DE1547A410FA8A333AC8F00223FA4089@RCSIEXCHANGE.rcsi-internal.ie> Content-Type: text/plain; charset="us-ascii" Hi , I plan isolate GFPtagged cells from cryosections via laser microdissection for proteomics but still I am struggling with not loosing the GFP signal. Does anyone has a good idea how to fix the signal so that afterwards proteomics is still possible? Thanks a lot, Lilja Lilja Thoenes Dept. of Neurodegeneration and Physiology Royal College of Surgeons in Ireland (RCSI) 123 St. Stephens Green, Dublin 2, Ireland Office: +353 1 402 2794 Fax: +353 1 402 2447 ------------------------------ Message: 7 Date: Sun, 11 Oct 2009 21:07:39 -0700 From: "Kiranjit Grewal" Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress To: Message-ID: <277641.14522.qm@smtp101.sbc.mail.sp1.yahoo.com> Content-Type: text/plain; charset="us-ascii" Hi, Anybody has anything to share regarding Pathos both good and bad. We are planning to get one. Also please share if you have any experience with Tissue Xpress. Thanks, Histotech in CA ------------------------------ Message: 8 Date: Mon, 12 Oct 2009 10:36:08 -0500 From: "del phillips" Subject: [Histonet] hematoxylin and nova red staining To: Cc: dphillips33@juno.com Message-ID: <000001ca4b52$2f60e080$8e22a180$@lsu.edu> Content-Type: text/plain; charset="us-ascii" If hematoxylin is place on a slide before the nova red is it too late to add the nova red after the hematoxylin? Thanks ------------------------------ Message: 9 Date: Tue, 13 Oct 2009 14:10:39 -0700 From: Leroy Brown Subject: [Histonet] looking for calibration tools for Optimax plus stainer To: Message-ID: Content-Type: text/plain; charset="iso-8859-1"; Does anyone have a set of calibration tools needed to calibrate my BioGenex Optimax stainer. Seems they no longer make these or sell time?? thanks LeRoy Brown Ht(ASCP) HTL www.histocs.com 360-966-7300 ------------------------------ Message: 10 Date: Tue, 13 Oct 2009 14:46:37 -0700 From: Patti Loykasek Subject: Re: [Histonet] looking for calibration tools for Optimax plus stainer To: Message-ID: Content-Type: text/plain; charset="US-ASCII" In reference to LeRoy's last statement below- if anyone is selling time please put me on the list for the maximum allowable! I haven't been able to figure out how to cram more hours in a day! And it's only Tuesday. Patti Loykasek > Does anyone have a set of calibration tools needed to calibrate my BioGenex > Optimax stainer. Seems they no longer make these or sell time?? > > thanks > > LeRoy Brown Ht(ASCP) HTL > > www.histocs.com > > 360-966-7300 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 11 Date: Tue, 13 Oct 2009 16:48:46 -0500 From: "Adam ." Subject: [Histonet] Isotype background To: histonet@lists.utsouthwestern.edu Message-ID: <858249120910131448v26622f8dtd5599dbbf64e67e4@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi all, I am trying some IHC, and I am having a peculiar problem. Like I expect, my antibody of interest (anti-mouse goat polyclonal) stains nonspecifically at high concentrations (10 ug / ml) but as I titer it down (3 ug / mL), it seems to stain relatively specifically the cells I think it should stain. However, at 3 ug / mL, my isotype goat IgG stains nearly everything. Here is my protocol 1) Block in 3% H2O2 for 10'. Wash. 2) Block in 10% donkey serum for 1 hr. Wash. 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits) 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash. 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash. 7) Incubate with DAB+ (Dako) for 5'. For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson. Some people have suggested that I just do away with isotypes altogether and use a no primary control instead. I think there is some merit to this idea, but I still think my issue might be indicative of a larger technical problem in my staining protocol. Thanks, Adam ------------------------------ Message: 12 Date: Tue, 13 Oct 2009 19:10:08 -0700 From: "Cathy" Subject: RE: [Histonet] Automated H&E stainers To: "'Bauer, Karen L.'" , "'Connolly, Brett M'" , Message-ID: <9EFA5FE9872A49718A169C823AD49321@your8ba846406f> Content-Type: text/plain; charset="us-ascii" Have you had any problems with the Coverslipper? We purchased the Prisma with linked Coverslipper last March and have had on going problems with the Coverslipper jamming with error code -Motor ejector error E15. We have contacted Somagen and were told that this is normal. It is happening at least once a day and sometimes more. We love the stainer and if the Coverslipper wasn't jamming we would love it just as much. What do you do with the coverslipped slides? We find that they don't dry as quickly as they did on the older film Coverslipper. We don't have a vented sign out area so they are staying on the carousel until they are mostly dry. Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Monday, October 12, 2009 5:42 AM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automated H&E stainers Brett, We purchased the Tissue Tek PRISMA with the attached film coverslipper a year ago and LOVE IT!! Was a little nervous switching from the glass coverslips to the film, but the docs love the slides and we have had no problems. Very easy to use and we like the fact that we can file the slides the same day without having to wait for them to "dry". Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, October 09, 2009 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated H&E stainers Which one do you like?...pro&cons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 13 Oct 2009 21:52:57 CDT From: "DELIA GARCIA" Subject: RE: [Histonet] Transporting Formalin To: "Feher, Stephen" ,histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have various shipping addresses with our supplier. Which allows us to order directly for our clients. We incur all costs including the associated Hazardous Materials handling fee. We also use courier for in-town clients. Hope this helps. Delia Garcia Lead Histotechnologist Tucson, AZ -----Original Message----- Date: Tuesday, October 13, 2009 1:36:33 pm To: From: "Feher, Stephen" Subject: [Histonet] Transporting Formalin Hi gang, A question came up in a committee meeting today surrounding how other institutions are transporting quantities of 10% NBF from a main facility to outlying hospitals and outpatient clinics. The quantities in question ranged from 5 - 20 gallons of formalin in sealed carboy type containers. Does a main hospital or lab distribute the formalin to outlying facilities or are outlying facilities having to order direct from a supplier and bill the main facility? Some places just order it and transport via courier. This may be restricted due to various State EPA rules. How are you all doing this? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 14 Oct 2009 06:33:32 -0400 From: "Diana McCaig" Subject: [Histonet] lid gasket for processor To: Message-ID: Content-Type: text/plain; charset="us-ascii" Our lid gasket seemed to have grown overnight and expanded considerable in length. It is difficult to re-seat it back in. It appears too loose and when the processor cycles through a station and we open the lid, it is laying on the edge of the retort. Does anyone have any ideas what we can do until a new gasket can be ordered? Diana ------------------------------ Message: 15 Date: Wed, 14 Oct 2009 08:22:21 -0400 From: Melissa Mazan Subject: [Histonet] pkh To: histonet@lists.utsouthwestern.edu Message-ID: <4AD5C27D.5010601@tufts.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, We have collected a specific lung cell via FACS from mice, stained the cells with PKH, and then returned the cells to congenic mice via 1. tracheal injection or 2. tail vein injection. We can see the PKH positive cells in the recipient lungs on microscopy, but we are having a real problem costaining these cells with anything else such as vimentin - has anyone else tried this approach? Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu ------------------------------ Message: 16 Date: Wed, 14 Oct 2009 09:26:52 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] lid gasket for processor To: "Diana McCaig" , Message-ID: Content-Type: text/plain; charset="utf-8" You might be able to shrink it a little by putting the gasket in the refrigerator for a while. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Wednesday, October 14, 2009 6:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lid gasket for processor Our lid gasket seemed to have grown overnight and expanded considerable in length. It is difficult to re-seat it back in. It appears too loose and when the processor cycles through a station and we open the lid, it is laying on the edge of the retort. Does anyone have any ideas what we can do until a new gasket can be ordered? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr ------------------------------ Message: 17 Date: Wed, 14 Oct 2009 06:30:14 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress To: histonet@lists.utsouthwestern.edu Message-ID: <605622.63517.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 --- On Wed, 10/14/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Pathos Tissue Processor vs Tissue Xpress To: "Kiranjit Grewal" Date: Wednesday, October 14, 2009, 9:28 AM Under separate cover I am sending you an article where both instruments (as well as some others) are compared in?reurn/investment and effectiveness. Ren? J. --- On Mon, 10/12/09, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress To: histonet@lists.utsouthwestern.edu Date: Monday, October 12, 2009, 12:07 AM Hi, Anybody has anything to share regarding Pathos both good and bad. We are planning to get one. Also please share if you have any experience with Tissue Xpress. Thanks, Histotech in CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 14 Oct 2009 10:20:41 -0400 From: Merced M Leiker Subject: Re: [Histonet] Isotype background To: "Adam ." , histonet@lists.utsouthwestern.edu Message-ID: <31878536799288738D402C8A@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed I wouldn't get rid of the isotype control altogether; it's fulfilling it's purpose in trying to tell you something. Have you tried a no-primary control in parallel with your isotype control to see what the secondary is binding to - the tissue or bound goat IgG? What type of tissue is it? Maybe the goat IgG is binding to Fc receptors in the tissue. Try an Fc block. Or try a different goat IgG or one from a different company. But it may not even be a big issue; your goat IgG is not giving you the specific stain pattern that you are observing with your primary, right? It's just giving a lot of background? Instead of spending a lot of time troubleshooting the high background issue with your isotype control, it's already succeeded in telling you that your primary is specific, which is what you ultimately wanted to know, so you could just take that for what it's worth and go from there... Just my two cents' worth! Regards, Merced --On Tuesday, October 13, 2009 4:48 PM -0500 "Adam ." wrote: > Hi all, > > I am trying some IHC, and I am having a peculiar problem. Like I expect, > my antibody of interest (anti-mouse goat polyclonal) stains > nonspecifically at high concentrations (10 ug / ml) but as I titer it > down (3 ug / mL), it seems to stain relatively specifically the cells I > think it should stain. However, at 3 ug / mL, my isotype goat IgG stains > nearly everything. > > Here is my protocol > 1) Block in 3% H2O2 for 10'. Wash. > 2) Block in 10% donkey serum for 1 hr. Wash. > 3) Block in avidin 15', wash, biotin 15', wash (Vector Labs blocking kits) > 4) Incubate with primary / isotype overnight at 4C in 2% donkey serum. > Wash. 5) Incubate with secondary (biotinylated donkey anti-goat, cross > adsorbed to mouse) in 2% donkey serum for 1 hr at room temp. Wash. > 6) Incubate with strepavidin HRP in TBS-T for 30'. Wash. > 7) Incubate with DAB+ (Dako) for 5'. > > For isotype, I am using Chrompure Goat IgG, whole molecule from Jackson. > Some people have suggested that I just do away with isotypes altogether > and use a no primary control instead. I think there is some merit to this > idea, but I still think my issue might be indicative of a larger > technical problem in my staining protocol. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 19 Date: Wed, 14 Oct 2009 07:22:38 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress To: Donna Willis Cc: histonet@lists.utsouthwestern.edu Message-ID: <158309.9167.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Donna: Thank you for your comment. As you write if the Pathos Classic is still available what I wrote is still valid. Unfortunately when somebody writes an article the conclusions remain valid as long as the subjects don't change, but the approach remains. As you can see I have forwarded your comments and my answer for all to read in HistoNet so they can benefit of it. As to going to Kalamazzo I really appreciate your invitation but I seldom travel ("retired with a fixed income", you the drill!). Regards Ren? J. --- On Wed, 10/14/09, Donna Willis wrote: From: Donna Willis Subject: RE: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress To: "Rene J Buesa" Date: Wednesday, October 14, 2009, 9:58 AM Rene, There are now 2 Pathos units available to customers.? The Pathos Classic and the Pathos Delta.? You article was written before the Delta was on the market.? Please understand that giving this article to customers may not give them a true picture of the unit they are looking to purchase.? If you are interested in coming to the Milestone Kalamazoo office to experience the whole Milestone instrument line, we would love to have you. Sincerely, Donna Willis, HT/HTL(ASCP) North American Application Manager Milestone Medical (866) 995-5300 toll free (269) 488-4040 fax www.milestonemed.com Helping Patients -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, October 14, 2009 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress --- On Wed, 10/14/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Pathos Tissue Processor vs Tissue Xpress To: "Kiranjit Grewal" Date: Wednesday, October 14, 2009, 9:28 AM Under separate cover I am sending you an article where both instruments (as well as some others) are compared in?reurn/investment and effectiveness. Ren? J. --- On Mon, 10/12/09, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] Pathos Tissue Processor vs Tissue Xpress To: histonet@lists.utsouthwestern.edu Date: Monday, October 12, 2009, 12:07 AM Hi, Anybody has anything to share regarding Pathos both good and bad. We are planning to get one. Also please share if you have any experience with Tissue Xpress. Thanks, Histotech in CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 14 Oct 2009 10:25:51 -0400 From: kkwaa@bidmc.harvard.edu Subject: [Histonet] HerCep To: histonet@lists.utsouthwestern.edu Message-ID: <1453B63A4BE95441AA65F577CEE7B6E104E16997F0@EVS5CCR.its.caregroup.org> Content-Type: text/plain; charset=us-ascii Does anyone have any experience using the Dako HerCep kit? And do you have any problems with cytoplasmic staining? ------------------------------ Message: 21 Date: Wed, 14 Oct 2009 11:24:44 -0400 From: susan bryant Subject: [Histonet] unsubscribe, please To: histonet@lists.utsouthwestern.edu Message-ID: <4AD5ED3C.20805@labpath.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I wish to unsubscribe and return in 6 weeks. Thank you, Susan E. Bryant Knoxville Dermatopathology ------------------------------ Message: 22 Date: Wed, 14 Oct 2009 11:42:07 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Histology Job Alert Histotechnologist needed in Atlanta To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! I have a new position that I want to let everyone know about. I am assisting a client located in Atlanta GA that is in need of a histotechnologist. This is a full time permanent position on the night shift (12:30a-8:30a). My client offers excellent benefits and compensation and a generous shift differential. ASCP certification and strong experience in grossing is required. For more information please contact Pam Barker at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 23 Date: Wed, 14 Oct 2009 12:34:59 -0400 From: Emily Sours Subject: Re: [Histonet] unsubscribe, please To: susan bryant , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Good for you! You should probably FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE WRITES IN TO UNSUBSCRIBE. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum On Wed, Oct 14, 2009 at 11:24 AM, susan bryant wrote: > I wish to unsubscribe and return in 6 weeks. > > Thank you, > Susan E. Bryant > Knoxville Dermatopathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 24 Date: Wed, 14 Oct 2009 12:56:08 -0400 From: Peter Carroll Subject: Re: [Histonet] unsubscribe, please To: Emily Sours Cc: histonet@lists.utsouthwestern.edu, susan bryant Message-ID: <4AD602A8.6050306@umdnj.edu> Content-Type: text/plain; charset=UTF-8; format=flowed >FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE WRITES IN TO UNSUBSCRIBE Histonet Fun-Fact: Hits for the word "unsubscribe" in the archive: 1144 Hits for the word "eosin" in the archive: 801 Perhaps we should rename this list "Unsubscribenet" ;) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 71, Issue 14 **************************************** This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Shanqin.Xu <@t> immunogen.com Thu Oct 15 15:39:35 2009 From: Shanqin.Xu <@t> immunogen.com (Xu, Shanqin) Date: Wed Oct 21 12:12:59 2009 Subject: [Histonet] IHC protocols for GPI-linked proteins Message-ID: <1EC9E4133CB4EB4A90383226A9864614015CD23CE2@wal-mail02.Immunogen.com> Hi All, I've been searching for special IHC protocols for GPI-linked proteins to try to hold specific staining at the plasma membrane, but I haven't found anything useful. Would you have any suggestion that might be able to solve this problem? It would be very nice to here from you. Thanks, Shanqin From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 15 18:44:59 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Oct 21 12:13:01 2009 Subject: [Histonet] Wet cassette transport containers? Message-ID: <1AAF670737F193429070841C6B2ADD4CF71852C8@EXMBMCB15.ucsfmedicalcenter.org> Hi, We're looking for rectangular containers to transport Sakura tissue processor cassette baskets filled with wet-tissue cassettes. The container has to be water-tight as it will have formalin in it. Is anyone using a container that fits those criteria? Everything we've tried so far leaks (various plastic locking food containers, locking water-tight equipment containers). They have to be leak proof despite being tilted or turned sideways for 30-60 minutes during transport. Thanks for any help! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 16 12:10:18 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Oct 21 12:13:04 2009 Subject: [Histonet] Wet block transport containers? Message-ID: Hi, We?re looking for rectangular containers to transport Sakura tissue processor cassette baskets filled with wet-tissue cassettes. The container has to be water-tight as it will have formalin in it. Is anyone using a container that fits that criteria? Everything we?ve tried so far leaks (various plastic locking food containers, locking water-tight equipment containers) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org From simmca <@t> UPMC.EDU Wed Oct 21 09:35:04 2009 From: simmca <@t> UPMC.EDU (Simmons, Christopher) Date: Wed Oct 21 12:13:05 2009 Subject: [Histonet] Leica Auto Casette Writer Message-ID: Dear Histonetters, Has anyone experienced any issues (adverse) with the Leica casette writer? Any issues with scanning the barcodes it produces? Any issues with the ink spilling? Thank you all! Chris Simmons, B.S., A.S., HTL(ASCP) Lead Technologist PUH Histology 412-647-7660 desk 13242 pager From Melanie.Black <@t> uct.ac.za Mon Oct 19 08:17:29 2009 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Wed Oct 21 12:13:10 2009 Subject: [Histonet] Re: looking for CD31 for rat tissues In-Reply-To: References: Message-ID: <42BDD3A4-B614-4BC5-B263-D01B6672B2E9@uct.ac.za> Hi Patsy Yes it works very well. The antibody is cat # RDI-RTCD31-3A12 from Fitzgerald. Yes it is zinc fixed. You use alk phos instead of DAB. See pic. Zinc Fixative (JB Fixative or ZSF) 0.1M Tris Buffer, pH 7.4 Tris Base -------------------------------- 12.1 g (TRIZMA) 1N HCL ----------------------------------- 81.5 ml Distilled water -------------------------- 900 ml Mix to dissolve. Adjust pH to 7.4 Zinc Fixative Calcium Acetate ---------------------- 0.5 g Zinc Acetate -------------------------- 5.0 g Zinc Chloride -------------------------- 5.0 g 0.1M Tris Buffer made above ------ 1000 ml Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution. Store Zinc Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation longer than 48 hours may make the tissue brittle and difficult to cut. ? Good luck Melanie. On 19 Oct 2009, at 2:57 PM, Patsy Ruegg wrote: > > > Melanie, > > I am very interested in your CD31 protocol, does it work on rat > tissue, do > you have to fix in zinc fixative? > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Melanie > Black > Sent: Monday, October 19, 2009 12:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: [Histonet] Rabbit-on-Rodent HRP polymer > > Hi Jen > > In reply to your question on the Biocare Rabbit-on rodent HRP, you > are correct. I include the protocol I use, the only difference is > mine is Alp phos linked instead of HRP. I use rat tissue and CD 31 > antibody (from Fitzgerald) that specifically requires zinc fixed > tissue. For the zinc fixation, I use exactly the same formula that > Gayle Callis sent out. > > > CD 31 Alk Phos Method for Zinc fixed Tissue. > > Biocare Medical Kit. > > > > > Dewax slides in xylene. > Take slides thru alcohol to water, and into tris buffer. > Incubate with Rodent Block R (RBR962H) from Biocare Medical, for 30 > mins. > Wash in TBS X 2. > Incubate with Primary antibody for 30 mins.(1:50) - diluted in 1% BSA > in PBS. Anti Rat CD 31 from Fitzgerald. > Wash in TBS X 2. > Incubate in Mouse-on-Rat AP-Polymer (MRT623H), Biocare Medical, for > 20 mins. > Wash in TBS X 2. > Add a drop of NBT(DAKO - K0598), and check for colour development. > (YOU WILL USE DAB) > Rinse in water, dehydrate clear and mount. > > NB: Sections must be cut onto UNCOATED slides, and incubated on a 60 > degree hot plate for 30 mins, followed by overnight incubation at 37 > degrees. > > Hope this helps > Regards > Melanie. > > > > Hi All, > > I recently purchased a rabbit-on-rodent HRP polymer from Biocare. > I will be using this on mouse tissue, using a rabbit polyclonal CD3 > as my primary. I just wanted to clarify a couple things about the > protocol before I try it out tomorrow and was wondering if anyone > could offer some assistance. First of all, I should aske if anyone > has a protocol for this? What I really would like to make sure is > that I will not have to use a label after the rabbit-on-rodent > secondary ab. I believe that I will just have to apply my blocking > agent, avidin/biotin blocks, primary antibody, secondary anitbody, > followed by chromogen, right? My blocking agent is made up of casein > and some other proprietary agents in a phosphate buffer (it is non- > serum) so I was told that should work fine. Does this sound > correct? Thanks in advance! I haven't been able to get through to > tech support all day, so hopefully you will have some answers for me! > > Jen > > > Melanie Black > 082 469 3352 > > Cardiovascular Research Unit > 3rd Floor; Chris Barnard Building > Medical School; > Observatory. 7925. > University of Cape Town. > South Africa. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 20 13:29:03 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Oct 21 12:13:12 2009 Subject: [Histonet] RE: Antigen retrieval question In-Reply-To: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> Message-ID: <1AAF670737F193429070841C6B2ADD4CF72820A6@EXMBMCB15.ucsfmedicalcenter.org> Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BFicher <@t> chomp.org Wed Oct 21 12:32:44 2009 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Wed Oct 21 12:32:55 2009 Subject: [Histonet] Wet cassette transport containers? In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF71852C8@EXMBMCB15.ucsfmedicalcenter.org> References: <1AAF670737F193429070841C6B2ADD4CF71852C8@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: Tim, We have also been using the locking plastic containers that have a rubber gasket around the lid. We place the Sakura rack inside of this container and then place it in a larger container of the same design and this is placed in a third container or an ice chest with formalin neutralizing pads. The spillage if slight is well contained. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, October 15, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wet cassette transport containers? Hi, We're looking for rectangular containers to transport Sakura tissue processor cassette baskets filled with wet-tissue cassettes. The container has to be water-tight as it will have formalin in it. Is anyone using a container that fits those criteria? Everything we've tried so far leaks (various plastic locking food containers, locking water-tight equipment containers). They have to be leak proof despite being tilted or turned sideways for 30-60 minutes during transport. Thanks for any help! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From Simoskevitz <@t> Osteotech.com Wed Oct 21 12:42:28 2009 From: Simoskevitz <@t> Osteotech.com (Simoskevitz@Osteotech.com) Date: Wed Oct 21 12:42:10 2009 Subject: [Histonet] Trichrome Stains on MMA thin sections Message-ID: I am having problems with both the Goldners and Massons Trichrome on my MMA sections. They are ground to between 20 and 30 microns, but the staining is nonspecific. It stains some of the bone the correct color, but not the rest of it. Any and all help would be greatly appreciated. Thanks, Ricki ********************************************* Ricki Simoskevitz, Research Associate III Osteotech Inc. 51 James Way Eatontown, NJ 07724 Phone: (732) 542-2800 X6328 Fax: (732) 935-1298 ********************************************** From marilyngamble <@t> comcast.net Wed Oct 21 13:07:20 2009 From: marilyngamble <@t> comcast.net (marilyngamble@comcast.net) Date: Wed Oct 21 13:07:22 2009 Subject: [Histonet] Leica Imaging Workstation Q550MW Message-ID: <74442514.699311256148440079.JavaMail.root@sz0135a.westchester.pa.mail.comcast.net> If anyone has a specification sheet (not brochure) for the Leica Q550MW Imaging system, would you contact me directly??? The information I'm looking for is dimensions (W/D/H),?weight , electrical information, etc. All I've found is the brochure which gives none of the information that I need. Thanks, Marilyn From mchavarria <@t> coreplus.com Wed Oct 21 13:26:52 2009 From: mchavarria <@t> coreplus.com (Marbella Chavarria) Date: Wed Oct 21 13:26:11 2009 Subject: [Histonet] Leica Auto Casette Writer In-Reply-To: References: Message-ID: We are using that printer and works well for us. Regards, Marbella Chavarria BS, HTL (ASCP) Laboratory Supervisor CorePlus, LLC. 9450 S.W. 72nd Street Miami, FL 33173 Main: 305-265-8300 ext 128 Fax: 786-924-0277 E-Mail: mchavarria@coreplus.com visit us online at: www.coreplus.com CorePlus is Accredited by The Joint Commission -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Simmons, Christopher Sent: Wednesday, October 21, 2009 10:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica Auto Casette Writer Dear Histonetters, Has anyone experienced any issues (adverse) with the Leica casette writer? Any issues with scanning the barcodes it produces? Any issues with the ink spilling? Thank you all! Chris Simmons, B.S., A.S., HTL(ASCP) Lead Technologist PUH Histology 412-647-7660 desk 13242 pager _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Oct 21 14:00:52 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Oct 21 14:00:57 2009 Subject: [Histonet] Re: Fite control slides Message-ID: The Fite (or Fite-Faraco) stain is a light-microscopic acid-fast stain with a different acid-fast extraction routine. (I don't know if it's ever been adapted for fluorescence microscopy, but it certainly could be.) The Fite stain is used to demonstrate Mycobacterium leprae (the etiologic agent of Hansen disease) which is less acid-fast than Mycobacterium leprae. Tissue containing known Mycobacterium leprae is needed for the control slide. Obviously, human tissue is difficult to obtain. The armadillo (North American nine-banded armadillo, Dasypus novemcinctus) is both naturally infected with M. leprae (in Louisiana), and is easily infected experimentally. The etiologic agent is known to be identical to the human agent. It would seem to me that infected armadillo tissue would be an acceptable positive control. Does anyone have it available? Bob Richmond Samurai Pathologist Knoxville TN From arvidsonkristen <@t> yahoo.com Wed Oct 21 14:15:35 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Oct 21 14:15:39 2009 Subject: [Histonet] Anyone CLIA Certified? Message-ID: <626661.28093.qm@web65709.mail.ac4.yahoo.com> We are a derm lab who is CLIA certified.? We are going through some admin changes and I have been reading through (and learning) clia, osha, chemical hygiene etc? manuals and noticing they all seem outdated.? I am a lab sup that is responsible for all this and not quite sure how to go about it all.? My main question here is how do you write a CLIA manual?? I've been on the web site and I don't know where to begin. From Margaret.Perry <@t> sdstate.edu Wed Oct 21 14:20:55 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Oct 21 14:22:19 2009 Subject: [Histonet] Warthin Faulkner method for spirochaetes Message-ID: We are trying to work up this stain per pathologists special request. We usually do the Warthin-Starry method not this modification. I have the published article from Stain Technology Vol. 20 No. 3 July 1945. The protocol is a little vague and I have some questions? Step one brings the slides into acetate buffer. Step two says ?impregnate sections 45 minutes at 55-60? C (in paraffin oven) in 1% AGNO3 in water of the same buffer level. What does water of the same buffer level mean? We interpret this as the same acetate buffer as in step one. Is this correct? Also it says to use 1 :500 acid gold chloride(HAuCl4.xH2O) to tone. Can we substitute 0.2%AuCl1? Thanks for your help. Margaret Perry South Dakota State University From sbreeden <@t> nmda.nmsu.edu Wed Oct 21 14:27:30 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Oct 21 14:27:37 2009 Subject: [Histonet] Stainer programming thanks Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BB0@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to several of you that responded to my "how-to-program-my-stainer" question. I had a call from Carolyn Doan of Leica, who walked me through the (very simple - wouldn't you know?) programming and I was up and running in about 30 seconds. Thank you to all who advised me on copying and using one program to create another. Histonet rocks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Wed Oct 21 14:31:29 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 21 14:31:33 2009 Subject: [Histonet] Uranyl nitrate In-Reply-To: <4ADF0687.7400.0077.1@harthosp.org> Message-ID: <184679.50164.qm@web65702.mail.ac4.yahoo.com> Under separate cover I am sending what you are looking for. Ren? J. --- On Wed, 10/21/09, Richard Cartun wrote: From: Richard Cartun Subject: [Histonet] Uranyl nitrate To: "Histonet" Date: Wednesday, October 21, 2009, 1:03 PM Our Safety people are asking us to stop using uranyl nitrate which I believe is used in our Dieterle and Reticulin histochemical stains.? Are there alternative staining protocols that do not use uranyl nitrate (forgive me because this is not my area of expertise)?? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT? 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From tjasper <@t> copc.net Wed Oct 21 14:38:12 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Oct 21 14:38:17 2009 Subject: [Histonet] Floor Cleaning Message-ID: <90354A475B420441B2A0396E5008D4965E308E@copc-sbs.COPC.local> Hi Folks, Got a floor cleaning question for you. We are transitioning to a new floor cleaning crew in our histo lab. I've been asked by the building manager to elicit some opinions about products to use and/or techniques to best get paraffin up off of linoleum. We were fine with our previous cleaners, however, the thinking around here is maybe it could be done better? Is a machine required or not? Could we do it at a lesser cost, and in a more bio-friendly way? Is there an easier and simpler method? Anyway this certainly is not the hot issue of the day for Histo-net. However if anyone would care to share anything on this it would be appreciated. Thanks, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net From b-frederick <@t> northwestern.edu Wed Oct 21 15:01:59 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 21 15:02:33 2009 Subject: [Histonet] Re: Fite control slides In-Reply-To: Message-ID: <64E2E9EDD9A04A86A58F2157F8C6B28A@lurie.northwestern.edu> NSH may have some in their control bank. Newcomer Supply sells control slides. Sigma may also. BErnice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, October 21, 2009 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Fite control slides The Fite (or Fite-Faraco) stain is a light-microscopic acid-fast stain with a different acid-fast extraction routine. (I don't know if it's ever been adapted for fluorescence microscopy, but it certainly could be.) The Fite stain is used to demonstrate Mycobacterium leprae (the etiologic agent of Hansen disease) which is less acid-fast than Mycobacterium leprae. Tissue containing known Mycobacterium leprae is needed for the control slide. Obviously, human tissue is difficult to obtain. The armadillo (North American nine-banded armadillo, Dasypus novemcinctus) is both naturally infected with M. leprae (in Louisiana), and is easily infected experimentally. The etiologic agent is known to be identical to the human agent. It would seem to me that infected armadillo tissue would be an acceptable positive control. Does anyone have it available? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed Oct 21 15:08:57 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Oct 21 15:10:24 2009 Subject: [Histonet] RE: Antigen retrieval question Message-ID: If we need to hold retrieved slides for awhile we hold them in water up to 4 hours. Holding in water overnight leads to no signal. We have also tried holding the slides in our Tris buffer and have found decreased or no staining. Margaret Perry From jiaya <@t> bme.ogi.edu Wed Oct 21 15:26:58 2009 From: jiaya <@t> bme.ogi.edu (jiaya@bme.ogi.edu) Date: Wed Oct 21 15:27:02 2009 Subject: [Histonet] withdrawal Message-ID: <20091021132658.2ivwzh618gokkgks@mail.bme.ogi.edu> Hi Please help me to withdrawal from the histonet. I am frustrated to see a huge amount email each day. Thanks yali From MadaryJ <@t> MedImmune.com Wed Oct 21 16:06:45 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Oct 21 16:06:51 2009 Subject: [Histonet] one day call for resumes Gaithersburg MD Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A138E4AE0@MD1EV002.medimmune.com> For anyone interested in a Pathology Associate position at Medimmune in Gaithersburg Md, I am looking at resumes on Thursday of this week and will call folks Friday if interested. I just want to see if there is that special someone out there that I have missed over the past few months. The job requires a degree or ASCP pref both and some decent experience like 10 yrs or more would be great. If a person could perform necropsy on rodents, trim the tissue, process, embed, cut stain with HE, specials and IHC, all with confidence and a high level of trouble shooting experience that would be great. The original position was for a PA 2 or 3 but we have made it a PA 1 now. I also need the person to really understand how to maintain equipment, recycle, not be wasteful, flexible on time and be an asset on the lab not a drain. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From stamptrain <@t> yahoo.com Wed Oct 21 16:38:09 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed Oct 21 16:38:12 2009 Subject: [Histonet] withdrawal In-Reply-To: <20091021132658.2ivwzh618gokkgks@mail.bme.ogi.edu> References: <20091021132658.2ivwzh618gokkgks@mail.bme.ogi.edu> Message-ID: <625942.35319.qm@web55801.mail.re3.yahoo.com> The correct request is to "unsubscribe" but since you forgot the original information you received when you first subscribed or have failed to read any of the multitude of recent messages about unsubscribing, I'll guess you'll just have to suffer along with the rest of us in getting too many messages. Roger Moretz, Ph.D. ----- Original Message ---- From: "jiaya@bme.ogi.edu" To: histonet@lists.utsouthwestern.edu Sent: Wed, October 21, 2009 4:26:58 PM Subject: [Histonet] withdrawal Hi Please help me to withdrawal from the histonet. I am frustrated to see a huge amount email each day. Thanks yali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Wed Oct 21 16:44:01 2009 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Oct 21 16:44:05 2009 Subject: [Histonet] withdrawal In-Reply-To: <625942.35319.qm@web55801.mail.re3.yahoo.com> References: <20091021132658.2ivwzh618gokkgks@mail.bme.ogi.edu> <625942.35319.qm@web55801.mail.re3.yahoo.com> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316B786A52E@JHEMTEXVS3.win.ad.jhu.edu> To subscribe or unsubscribe to the list or change your subscription to the daily digest mode etc. you need to go to: http://lists.utsouthwestern.edu/mailman/listinfo/histonet or email: Histonet-requests@lists.utsouthwestern.edu with the word "help" (without quotation marks) in the subject line or body of the message. Instructions will be returned to you by email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Moretz Sent: Wednesday, October 21, 2009 5:38 PM To: jiaya@bme.ogi.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] withdrawal The correct request is to "unsubscribe" but since you forgot the original information you received when you first subscribed or have failed to read any of the multitude of recent messages about unsubscribing, I'll guess you'll just have to suffer along with the rest of us in getting too many messages. Roger Moretz, Ph.D. ----- Original Message ---- From: "jiaya@bme.ogi.edu" To: histonet@lists.utsouthwestern.edu Sent: Wed, October 21, 2009 4:26:58 PM Subject: [Histonet] withdrawal Hi Please help me to withdrawal from the histonet. I am frustrated to see a huge amount email each day. Thanks yali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed Oct 21 17:15:18 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Oct 21 17:15:29 2009 Subject: [Histonet] Inspection question Message-ID: Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From JWeems <@t> sjha.org Wed Oct 21 17:17:18 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 21 17:17:27 2009 Subject: [Histonet] Inspection question In-Reply-To: References: Message-ID: No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From tjasper <@t> copc.net Wed Oct 21 17:29:25 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Oct 21 17:29:30 2009 Subject: [Histonet] Inspection question References: Message-ID: <90354A475B420441B2A0396E5008D4967319D8@copc-sbs.COPC.local> Furthermore, most any plant (unless I'm missing something here) is beneficial for air quality...plants want CO2 and we appreciate their oxygen. I sure hope some inspector somewhere hasn't taken issue with plants your in the lab. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 21, 2009 3:17 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Inspection question No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bbroders <@t> unlnotes.unl.edu Wed Oct 21 17:47:05 2009 From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen) Date: Wed Oct 21 17:47:18 2009 Subject: [Histonet] Autostainers For sale Message-ID: Hope it is ok on this listserv to announce items like this. Three Dako Autostainers for sale. Complete with computers. About 7 years old. Price negotiable. Contact me by email. Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From amosbrooks <@t> gmail.com Wed Oct 21 17:55:45 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Oct 21 17:55:53 2009 Subject: [Histonet] Digest replies Message-ID: <582736990910211555l99e4228sf4edccd06c79667d@mail.gmail.com> Wow, I'm tellin ya the next person that replies to a digest without trimming...after the day I've had I can't be responsible for the ball of flame that may be hurled! GRRR, Amos -- Sent from my mobile device From CIngles <@t> uwhealth.org Wed Oct 21 18:30:30 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Oct 21 18:31:46 2009 Subject: [Histonet] Inspection question References: <90354A475B420441B2A0396E5008D4967319D8@copc-sbs.COPC.local> Message-ID: Not to mention a little organic bit of the outside world when your stuck inside a white-washed cube all day... Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Wed 10/21/2009 5:29 PM To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inspection question Furthermore, most any plant (unless I'm missing something here) is beneficial for air quality...plants want CO2 and we appreciate their oxygen. I sure hope some inspector somewhere hasn't taken issue with plants your in the lab. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 21, 2009 3:17 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Inspection question No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Oct 21 18:35:13 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Wed Oct 21 18:36:05 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUmU6IGxvb2tpbmcgZm9yIENEMzEgZm9yIHJhdCB0aXNzdWVz?= References: , , <42BDD3A4-B614-4BC5-B263-D01B6672B2E9@uct.ac.za> Message-ID: <200910220735124787572@foxmail.com> after Zinc fixation what should you do before cutting sections on a cryostat/microtome? need sucrose cryoprotection as well? 2009-10-22 TF ???? Melanie Black ????? 2009-10-22 01:16:43 ???? Patsy Ruegg ??? histonet ??? [Histonet] Re: looking for CD31 for rat tissues Hi Patsy Yes it works very well. The antibody is cat # RDI-RTCD31-3A12 from Fitzgerald. Yes it is zinc fixed. You use alk phos instead of DAB. See pic. Zinc Fixative (JB Fixative or ZSF) 0.1M Tris Buffer, pH 7.4 Tris Base -------------------------------- 12.1 g (TRIZMA) 1N HCL ----------------------------------- 81.5 ml Distilled water -------------------------- 900 ml Mix to dissolve. Adjust pH to 7.4 Zinc Fixative Calcium Acetate ---------------------- 0.5 g Zinc Acetate -------------------------- 5.0 g Zinc Chloride -------------------------- 5.0 g 0.1M Tris Buffer made above ------ 1000 ml Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution. Store Zinc Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation longer than 48 hours may make the tissue brittle and difficult to cut. ? Good luck Melanie. On 19 Oct 2009, at 2:57 PM, Patsy Ruegg wrote: > > > Melanie, > > I am very interested in your CD31 protocol, does it work on rat > tissue, do > you have to fix in zinc fixative? > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Melanie > Black > Sent: Monday, October 19, 2009 12:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: [Histonet] Rabbit-on-Rodent HRP polymer > > Hi Jen > > In reply to your question on the Biocare Rabbit-on rodent HRP, you > are correct. I include the protocol I use, the only difference is > mine is Alp phos linked instead of HRP. I use rat tissue and CD 31 > antibody (from Fitzgerald) that specifically requires zinc fixed > tissue. For the zinc fixation, I use exactly the same formula that > Gayle Callis sent out. > > > CD 31 Alk Phos Method for Zinc fixed Tissue. > > Biocare Medical Kit. > > > > > Dewax slides in xylene. > Take slides thru alcohol to water, and into tris buffer. > Incubate with Rodent Block R (RBR962H) from Biocare Medical, for 30 > mins. > Wash in TBS X 2. > Incubate with Primary antibody for 30 mins.(1:50) - diluted in 1% BSA > in PBS. Anti Rat CD 31 from Fitzgerald. > Wash in TBS X 2. > Incubate in Mouse-on-Rat AP-Polymer (MRT623H), Biocare Medical, for > 20 mins. > Wash in TBS X 2. > Add a drop of NBT(DAKO - K0598), and check for colour development. > (YOU WILL USE DAB) > Rinse in water, dehydrate clear and mount. > > NB: Sections must be cut onto UNCOATED slides, and incubated on a 60 > degree hot plate for 30 mins, followed by overnight incubation at 37 > degrees. > > Hope this helps > Regards > Melanie. > > > > Hi All, > > I recently purchased a rabbit-on-rodent HRP polymer from Biocare. > I will be using this on mouse tissue, using a rabbit polyclonal CD3 > as my primary. I just wanted to clarify a couple things about the > protocol before I try it out tomorrow and was wondering if anyone > could offer some assistance. First of all, I should aske if anyone > has a protocol for this? What I really would like to make sure is > that I will not have to use a label after the rabbit-on-rodent > secondary ab. I believe that I will just have to apply my blocking > agent, avidin/biotin blocks, primary antibody, secondary anitbody, > followed by chromogen, right? My blocking agent is made up of casein > and some other proprietary agents in a phosphate buffer (it is non- > serum) so I was told that should work fine. Does this sound > correct? Thanks in advance! I haven't been able to get through to > tech support all day, so hopefully you will have some answers for me! > > Jen > > > Melanie Black > 082 469 3352 > > Cardiovascular Research Unit > 3rd Floor; Chris Barnard Building > Medical School; > Observatory. 7925. > University of Cape Town. > South Africa. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Oct 21 18:36:06 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Oct 21 18:36:24 2009 Subject: [Histonet] RE: Antigen retrieval question References: Message-ID: <200910220736060954046@foxmail.com> aG9sZCBpbiBQQlMgYXMgbG9uZyBhcyBzZXZlcmFsIGRheXMgd2l0aG91dCBhbnkgcHJvYmxlbSwg dW5kZXIgNCBDLg0KDQoNCjIwMDktMTAtMjIgDQoNCg0KDQpURiANCg0KDQoNCreivP7Iy6O6IFBl cnJ5LCBNYXJnYXJldCANCreiy83Ksbzko7ogMjAwOS0xMC0yMiAgMDQ6MTQ6MzEgDQrK1bz+yMuj uiBoaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUgDQqzrcvNo7ogDQrW98zio7ogW0hp c3RvbmV0XSBSRTogQW50aWdlbiByZXRyaWV2YWwgcXVlc3Rpb24gDQogDQpJZiB3ZSBuZWVkIHRv IGhvbGQgcmV0cmlldmVkIHNsaWRlcyBmb3IgYXdoaWxlIHdlIGhvbGQgdGhlbSBpbiB3YXRlciB1 cCB0byA0IGhvdXJzLiAgSG9sZGluZyBpbiB3YXRlciBvdmVybmlnaHQgbGVhZHMgdG8gbm8gc2ln bmFsLiAgV2UgaGF2ZSBhbHNvIHRyaWVkIGhvbGRpbmcgdGhlIHNsaWRlcyBpbiBvdXIgVHJpcyBi dWZmZXIgYW5kIGhhdmUgZm91bmQgZGVjcmVhc2VkIG9yIG5vIHN0YWluaW5nLg0KTWFyZ2FyZXQg UGVycnkNCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQpI aXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0K aHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0 DQo= From Jason.PALMER <@t> svhm.org.au Wed Oct 21 19:11:57 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Wed Oct 21 19:12:39 2009 Subject: [Histonet] RE: Granzyme b and F4/80 Message-ID: For Serotec F4/80, we use 10% rabbit serum as block and 5% of the same as antibody diluent -our secondary is a biotinylated rabbit anti rat. We also retrieve with Dako proteinase K rather than citrate, with simple HRP-streptavidin then DAB, and it all works very nicely, no background issues. Hope this helps, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Hi All, I'm currently trying to work up Granzyme B and F4/80 on FFPE mouse tissue. I seemed to get a bit of non-specific staining for both and was wondering if there is any way I can eliminate some of this. For Granzyme B I am using a rabbit polyclonal from Abcam. My protocol goes like this: H202 block, citrate buffer HIER, Powerblock (casein-based, non-serum protein block), primary antibody, secondary antibody (rabbit-on rodent HRP polymer, from biocare), DAB, counterstain. My F4/80 is a rat anti-mouse primary from Serotec and the protocol is as follows: H202 block, citrate buffer HIER, Powerblock, avidin/biotin blocks, primary antibody, secondary antibody (Goat anti-rat, biotinylated, mouse-absorbed, from Biocare), HRP Label, DAB, counterstain. Is it true that if I switched to a serum block from the species in which the secondary was raised, instead of my non-serum protein block, it may help? Thank you in advance! Jennifer Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From wdesalvo.cac <@t> hotmail.com Wed Oct 21 23:06:35 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Oct 21 23:06:41 2009 Subject: [Histonet] Wet cassette transport containers? In-Reply-To: References: <1AAF670737F193429070841C6B2ADD4CF71852C8@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: We transport wet cassettes to the core lab and had used plastic containers, but the plastic always hardens, cracks and the cost to continually replace was prohibitive. We have had great success using the Kpak bag sealing system and use a heat sealer to seal the bag. The sealed bag works well with many different cassette racks, the amount of fixtive needed in the bag is minimized, we place the bag w/ rack in a hard sided cooler and when the "bag" arrives in the lab there is no spil and the removal of fixative and cassettes is more managable. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 21 Oct 2009 10:32:44 -0700 > From: BFicher@chomp.org > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Wet cassette transport containers? > CC: > > Tim, > We have also been using the locking plastic containers that have a > rubber gasket around the lid. We place the Sakura rack inside of this > container and then place it in a larger container of the same design and > this is placed in a third container or an ice chest with formalin > neutralizing pads. The spillage if slight is well contained. > > > R.Brian Fischer > Histology Lead Tech > Community Hospital of the Monterey Peninsula > PO Box HH Monterey Ca. 93942 > 831-625-4791 > Fax: 831-6583683 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, > Tim > Sent: Thursday, October 15, 2009 4:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wet cassette transport containers? > > Hi, > > We're looking for rectangular containers to transport Sakura tissue > processor cassette baskets filled with wet-tissue cassettes. The > container has to be water-tight as it will have formalin in it. Is > anyone using a container that fits those criteria? Everything we've > tried so far leaks (various plastic locking food containers, locking > water-tight equipment containers). They have to be leak proof despite > being tilted or turned sideways for 30-60 minutes during transport. > > Thanks for any help! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > Box 1656 > 1600 Divisadero St. > San Francisco, CA 94143-1656 > USA > > Phone: (415) 514-6042 > Pager: (415) 443-6509 > Fax: (415) 885-7409 > > Email: tim.morken@ucsfmedctr.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. > > Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/177141665/direct/01/ From ernestinemiddleton <@t> yahoo.ca Thu Oct 22 04:49:27 2009 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Thu Oct 22 04:49:32 2009 Subject: [Histonet] MOHL TECHNOLOGIST Message-ID: <227120.73037.qm@web51504.mail.re2.yahoo.com> Hi; Montefiore Medical Center is looking for someone to work on Tuesdays and Thursdays performing mohl surgery.? There will be about 6 cases er day and must be experience in the procedure.? Montefiore Medical Center is located in the Bronx , New York. If interest please contact: Ernestine Middleton 718-920-4157 or emiddlet@montefiore.org __________________________________________________________________ Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail. Click on Options in Mail and switch to New Mail today or register for free at http://mail.yahoo.ca From Heather.D.Renko <@t> osfhealthcare.org Thu Oct 22 08:38:01 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Oct 22 08:38:36 2009 Subject: [Histonet] Re: Leica Cassette Printer Message-ID: We have the Leica cassette printer in our facility too. Purchased this product a little over a year ago. Feel free to contact me directly for any information I can pass along to you. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From ree3 <@t> leicester.ac.uk Thu Oct 22 09:14:44 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Oct 22 09:18:32 2009 Subject: [Histonet] Inspection question/OT In-Reply-To: <90354A475B420441B2A0396E5008D4967319D8@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D4967319D8@copc-sbs.COPC.local> Message-ID: <7722595275A4DD4FA225B92CDBF174A18C865A1216@EXC-MBX3.cfs.le.ac.uk> Plants grown in the lab might include, blue lungwort, dead man's fingers, mistletoe, cancer weed, devil's gut, bonewort, kidney vetch, liverwort, Hawaii birdnest spleenwort and if you have the space how about a Haematoxylum campechianum?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 21 October 2009 23:29 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inspection question Furthermore, most any plant (unless I'm missing something here) is beneficial for air quality...plants want CO2 and we appreciate their oxygen. I sure hope some inspector somewhere hasn't taken issue with plants your in the lab. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 21, 2009 3:17 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Inspection question No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgill <@t> marylandgeneral.org Thu Oct 22 10:12:57 2009 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Oct 22 10:13:03 2009 Subject: [Histonet] Steiner stain on nexes Message-ID: <7E37482715185C4494F24AC3F17BE072018CCAFC@mdgen-exch.marylandgeneral.org> Hi, Has anyone had any issues or problems lately with their Steiner for H-pylori on the Nexes. We have been dealing with this for about 8 months or so. Our Slides are staining uneven and sometimes the organisms don't stain at all. We've sent slides to Ventana's complaint Dept. they duplicated the problem but can't help us solve the problem so far. CG __________________________________________________________________________________________________________________________ This message, together with any attachments, is confidential and intended only for the use of the individual or entity to which it is addressed. This information may be privileged and otherwise protected by state and federal law. If you are not the intended recipient,any disclosure, copying, distribution or any other use of the contents of this message is prohibited. If you have received this message in error, please notify the sender immediately by telephone or by return e-mail and delete this message along with any attachments. __________________________________________________________________________________________________________________________ From rjbuesa <@t> yahoo.com Thu Oct 22 11:07:56 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 22 11:08:00 2009 Subject: [Histonet] Inspection question In-Reply-To: Message-ID: <420019.27038.qm@web65713.mail.ac4.yahoo.com> Yes! Ren? J. --- On Wed, 10/21/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Inspection question To: "histonet" Date: Wednesday, October 21, 2009, 6:15 PM Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christen <@t> vet.k-state.edu Thu Oct 22 11:38:26 2009 From: christen <@t> vet.k-state.edu (Shelly Christenson) Date: Thu Oct 22 11:39:02 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: References: <90354A475B420441B2A0396E5008D4967319D8@copc-sbs.COPC.local> Message-ID: <4AE04432.EF71.003F.0@vet.k-state.edu> Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University From relia1 <@t> earthlink.net Thu Oct 22 11:43:48 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Oct 22 11:43:51 2009 Subject: [Histonet] RELIA Solutions Histology Careers Alert for Supervisors and Managers 10/21/09 Message-ID: Hi Histonetters! I have several brand new exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. Here are my BRAND NEW JOBS: Pathology Manager ? Portland, OR Pathology Supervisor ? Fresno, CA Histology Supervisor ? Los Angeles Histology Supervisor ? Spokane, WA hospital environment Histology Supervisor ? Austin, TX Histology Supervisor ? Corpus Christi, TX Pathology Supervisor ? Cape Cod, MA If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam RELIA is offering a $500 referral bonus for anyone you refer and I place and a $500 hiring bonus if I place you! There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia < http://www.myspace.com/pamatrelia> Follow me at www.twitter.com/pamatrelia From trathborne <@t> somerset-healthcare.com Thu Oct 22 11:43:46 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Oct 22 11:43:54 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: <4AE04432.EF71.003F.0@vet.k-state.edu> Message-ID: I would be interested in that list if you could find it., Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shelly Christenson Sent: Thursday, October 22, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants in the Histology Lab Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From carrolpb <@t> umdnj.edu Thu Oct 22 11:46:37 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Oct 22 11:46:47 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: References: Message-ID: <4AE08C6D.70806@umdnj.edu> Me too! Rathborne, Toni wrote: > I would be interested in that list if you could find it., > > Toni > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shelly > Christenson > Sent: Thursday, October 22, 2009 12:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Plants in the Histology Lab > > > Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. > > > Shelly Christenson HT(ASCP) > Veterinary Diagnostic Lab. > Kansas State University > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from > Somerset Medical Center and are intended only for the addressee. > The information contained in this message is confidential > and may contain privileged, confidential, proprietary and/or > trade secret information entitled to protection and/or exemption > from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is > strictly prohibited and may be unlawful. If you are not the > addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call > Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > -------------------------------------------------------------- > Somerset Medical Center is the recipient of the > 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, > the nation's leading health care ratings company. > > Visit Somerset Medical Center's Web site > - www.somersetmedicalcenter.com - > for news, event listings, health information and more. > > Join the Discussion: > Facebook: www.somersetmedicalcenter.com/fb > Twitter: www.twitter.com/SomersetMedCtr > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Oct 22 11:48:13 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 22 11:48:16 2009 Subject: [Histonet] Fw: Rene, favor to ask... Message-ID: <910159.15590.qm@web65704.mail.ac4.yahoo.com> There you have it! Ren? J. ken, Tim wrote: From: Morken, Tim Subject: Rene, favor to ask... To: "rjbuesa@yahoo.com" Date: Thursday, October 22, 2009, 12:44 PM Rene, I have a favor to ask. I am having trouble posting to histonet ? it keeps bouncing back, though I am receiving histonet emails. ? Could you post this question to histonet for me? I need some answers ASAP. ? Thanks! ************************************************** Hi all, ? Our Joint Commission audit was just completed (first time for JC for me). We passed almost everything fine.? ? The one thing they came up with is that we don?t use ?negative controls? for most of the special stains ? like trichrome, congo red, etc. We use negative controls for micro-organism cases but not for the others. In fact I?ve never heard of anyone doing that. ? Has anyone had this issue with JC? Does anyone run ?negative? controls for non-organism special stains? Frankly I?m not sure how that would be done for something like a trichrome or other purely tissue-element stains. Has anyone successfully explained to JC why we don?t run negative controls for general special stains? ? Thanks for any insight! ? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA Email: tim.morken@ucsfmedctr.org ? ******************************************************* ? ? From TMcNemar <@t> lmhealth.org Thu Oct 22 11:54:49 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Oct 22 11:55:27 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: <4AE04432.EF71.003F.0@vet.k-state.edu> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E147@lmhsmail.lmhealth.org> I don't know about any list but I can tell you that spider plants grow like crazy.... they like the formalin. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shelly Christenson Sent: Thursday, October 22, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants in the Histology Lab Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Oct 22 11:58:43 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Oct 22 11:58:52 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: References: Message-ID: <2B336C75-E288-43F8-BFE9-798DEE532AD0@email.arizona.edu> My spider plants are taking over my lab. I've supplied half the university with the little spiders. 8-) Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Oct 22, 2009, at 9:43 AM, Rathborne, Toni wrote: > I would be interested in that list if you could find it., > > Toni > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shelly > Christenson > Sent: Thursday, October 22, 2009 12:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Plants in the Histology Lab > > > Some time back I took a workshop on Plants in the Histology Lab. > Some where I have the list as to which plants are the most > beneficial in the lab and which chemical fumes they like best. I > don't remember who did the workshop. I will have to do a little > hunting for the paper, but My Mother-IN-Tongues really like the > lab's fumes they are 3 to 4 feet tall and I've divided them 3 times > now. > > > Shelly Christenson HT(ASCP) > Veterinary Diagnostic Lab. > Kansas State University > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from > Somerset Medical Center and are intended only for the addressee. > The information contained in this message is confidential > and may contain privileged, confidential, proprietary and/or > trade secret information entitled to protection and/or exemption > from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is > strictly prohibited and may be unlawful. If you are not the > addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call > Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > -------------------------------------------------------------- > Somerset Medical Center is the recipient of the > 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, > the nation's leading health care ratings company. > > Visit Somerset Medical Center's Web site > - www.somersetmedicalcenter.com - > for news, event listings, health information and more. > > Join the Discussion: > Facebook: www.somersetmedicalcenter.com/fb > Twitter: www.twitter.com/SomersetMedCtr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu Oct 22 12:03:25 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Oct 22 12:03:30 2009 Subject: [Histonet] Inspection question References: <420019.27038.qm@web65713.mail.ac4.yahoo.com> Message-ID: <90354A475B420441B2A0396E5008D4967319DB@copc-sbs.COPC.local> The reason for that being? Pray tell... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 22, 2009 9:08 AM To: histonet; Patti Loykasek Subject: Re: [Histonet] Inspection question Yes! Ren? J. --- On Wed, 10/21/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Inspection question To: "histonet" Date: Wednesday, October 21, 2009, 6:15 PM Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MTrego <@t> chesterriverhealth.org Thu Oct 22 12:32:29 2009 From: MTrego <@t> chesterriverhealth.org (Trego, Melanie) Date: Thu Oct 22 12:32:37 2009 Subject: [Histonet] Reference for Plants Message-ID: <05505FB3EF11404EAE54786F0B76B1720209CC5B@crhsmail.crhs.org> Hi, all. My workplace is probably going to mess with my spiders as well, even though the lineage started about 25 years ago in this building. Apparently, it is much better to seal windows shut and breathe whatever is growing in the duct work. Don't get me started. I have a copy of an article from Economic Botany: 38(2). 1984. PP. 224-228, concerning plants and formalin/formaldehyde. It was given to me by a gas monitoring tech. The plants mentioned as being best at the job are spider plants, golden pothos and nephthytis (I think Philodendron). M. Trego Chester River Hospital Center This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From bhewlett <@t> cogeco.ca Thu Oct 22 12:32:38 2009 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu Oct 22 12:32:49 2009 Subject: [Histonet] Fw: Rene, favor to ask... References: <910159.15590.qm@web65704.mail.ac4.yahoo.com> Message-ID: <38A7600645184F2CA7C60E3711554D34@mainbox> Hi Tim, You gotta luv this! Where do they find these auditors? I can understand the requirement for the use of negative stain controls for definable entities, but for general oversight and other differential general tissue element stains, I am at a loss! What would be required is a tissue with absolutely NO stainable entities (normal or abnormal), that could be fixed and processed identically to the test material and then stained in the same manner at the same time. If you could find such a thing, my hat is off to you! Oh wait, I just had a thought. Get all the auditors to donate their brains to science to be used for this purpose, they obviously have no stainable elements! You would then solve both problems; a negative control for this purpose and no mindless auditors. All the best, Bryan ----- Original Message ----- From: "Rene J Buesa" To: ; Sent: Thursday, October 22, 2009 12:48 PM Subject: [Histonet] Fw: Rene, favor to ask... There you have it! Ren? J. ken, Tim wrote: From: Morken, Tim Subject: Rene, favor to ask... To: "rjbuesa@yahoo.com" Date: Thursday, October 22, 2009, 12:44 PM Rene, I have a favor to ask. I am having trouble posting to histonet ? it keeps bouncing back, though I am receiving histonet emails. Could you post this question to histonet for me? I need some answers ASAP. Thanks! ************************************************** Hi all, Our Joint Commission audit was just completed (first time for JC for me). We passed almost everything fine. The one thing they came up with is that we don?t use ?negative controls? for most of the special stains ? like trichrome, congo red, etc. We use negative controls for micro-organism cases but not for the others. In fact I?ve never heard of anyone doing that. Has anyone had this issue with JC? Does anyone run ?negative? controls for non-organism special stains? Frankly I?m not sure how that would be done for something like a trichrome or other purely tissue-element stains. Has anyone successfully explained to JC why we don?t run negative controls for general special stains? Thanks for any insight! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA Email: tim.morken@ucsfmedctr.org ******************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Oct 22 12:40:31 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Oct 22 12:40:36 2009 Subject: [Histonet] B5 question Message-ID: <03CE10D9-6D7B-4B22-8CE8-FA70BE47EACD@email.arizona.edu> Quick question regarding using B5 and Zenkers fixatives and the registry exam... Can anybody tell me if questions on these techniques still being asked on the exam? I'm working on a tumor project and some of the blocks are ancient and the tissue was fixed in B5. So my student was asking about B5 and I was telling her about the treatment before staining for Zenkers and B5 and she said that hadn't been covered in her classes. So do they need to know this for the exam? Personally, it doesn't matter to me if they need to know it or not for the exam - I think they should know about it Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. From thomas.crowell <@t> novartis.com Thu Oct 22 12:53:33 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Oct 22 12:53:41 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 10/22/2009 and will not return until 10/23/2009. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From scampbell <@t> celligent.net Thu Oct 22 13:07:02 2009 From: scampbell <@t> celligent.net (Campbell, Sharon) Date: Thu Oct 22 13:07:37 2009 Subject: [Histonet] New cassette printer and Slide Writer Message-ID: Hello Histo-world, I am beginning the search for a new cassette printer and slide printer. I would appreciate any feedback on these items. Thank you. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line scampbell@celligent.net From lucy.zong <@t> gmail.com Thu Oct 22 13:43:07 2009 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Thu Oct 22 13:43:13 2009 Subject: [Histonet] Tissue Tek 5 dispenser Message-ID: <8daef62e0910221143g4d76665h2543aa7631df287a@mail.gmail.com> I am looking for a Tissue Tek 5 dispensing unit. Can anyone help me out? From MMargiotta <@t> bmhmc.org Thu Oct 22 13:48:40 2009 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Thu Oct 22 13:48:45 2009 Subject: [Histonet] RE: Plants in the Histology Lab Message-ID: We have a few african violets that are so beautiful and they flower all the time. We get a lot of compliments from the hospital staff. Michele -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Thursday, October 22, 2009 12:55 PM To: Shelly Christenson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Plants in the Histology Lab I don't know about any list but I can tell you that spider plants grow like crazy.... they like the formalin. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shelly Christenson Sent: Thursday, October 22, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants in the Histology Lab Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From JWatson <@t> gnf.org Thu Oct 22 13:55:26 2009 From: JWatson <@t> gnf.org (James Watson) Date: Thu Oct 22 13:55:29 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: References: <4AE04432.EF71.003F.0@vet.k-state.edu> Message-ID: Here is a link to a list based on a NASA studies for plants that absorb benzene, formaldehyde, trichloroethylene, xylene, and toluene. http://en.wikipedia.org/wiki/List_of_air-filtering_soil_and_plants James Watson HT? ASCP Facilities Manager of Histology GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, October 22, 2009 9:44 AM To: Shelly Christenson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Plants in the Histology Lab I would be interested in that list if you could find it., Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shelly Christenson Sent: Thursday, October 22, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants in the Histology Lab Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From akbitting <@t> geisinger.edu Thu Oct 22 13:56:53 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Oct 22 13:57:01 2009 Subject: [Histonet] anti-Tau on BenchmarkXT Message-ID: <4AE072B6.2B7F.00C9.0@geisinger.edu> I may have asked this before, but is anyone using BioCare's Tau antibody on their BenchmarkXT?? If so, would you be willing to share your experience with it? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From sbreeden <@t> nmda.nmsu.edu Thu Oct 22 14:01:41 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Oct 22 14:01:48 2009 Subject: [Histonet] Plants-in-the-lab OT Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BB8@nmdamailsvr.nmda.ad.nmsu.edu> I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lblazek <@t> digestivespecialists.com Thu Oct 22 14:15:23 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 22 14:10:47 2009 Subject: [Histonet] RE: Plants-in-the-lab OT In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46BB8@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46BB8@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Oct 22 14:24:43 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 22 14:19:54 2009 Subject: [Histonet] RE: Plants-in-the-lab OT In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF73A431B@EXMBMCB15.ucsfmedicalcenter.org> References: <4D14F0FC9316DD41972D5F03C070908B02E46BB8@nmdamailsvr.nmda.ad.nmsu.edu> <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> <1AAF670737F193429070841C6B2ADD4CF73A431B@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A8698B@IBMB7Exchange.digestivespecialists.com> Or all the mahogany on the walls. Linda Blazek ----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Thursday, October 22, 2009 3:18 PM To: Blazek, Linda; 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: RE: Plants-in-the-lab OT Or it could not handle the hot air in the admin office! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 22, 2009 12:15 PM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants-in-the-lab OT I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Thu Oct 22 14:19:24 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Oct 22 14:22:09 2009 Subject: [Histonet] Tissue Tek 5 dispenser References: <8daef62e0910221143g4d76665h2543aa7631df287a@mail.gmail.com> Message-ID: Try Tech One Biomedical. I am not sure what you are asking for. www.techoneweb.com Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lucy Zong Sent: Thu 10/22/2009 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Tek 5 dispenser I am looking for a Tissue Tek 5 dispensing unit. Can anyone help me out? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Oct 22 14:26:16 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Oct 22 14:26:22 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> Message-ID: <650541.91710.qm@web113818.mail.gq1.yahoo.com> Hi All, I think all of you are missing the point of Patti's question. ?She stated that her lab was dinged for having plants in the lab by a CAP inspector. ? I had the same thing happen to me years ago. ?The inspector stated that plants attract insects that can contaminate a supposedly clean environment. ? Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. ?I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific.? The powers that be made me remove it from the lab for an inspection.? It went to live in one of the administrator's office for several months.? And died!? I think it needed the fumes!? Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive.? The absorption of Fume Matter is a secondary, but beneficial, effect.? You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Oct 22 14:35:05 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 22 14:30:17 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: <650541.91710.qm@web113818.mail.gq1.yahoo.com> References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> <650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A8698D@IBMB7Exchange.digestivespecialists.com> Just what question on the CAP check list does this fall under? ________________________________ From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; Blazek, Linda; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL President Phoenix Lab Consulting Tele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jackdodo <@t> msn.com Thu Oct 22 14:31:10 2009 From: jackdodo <@t> msn.com (Jack Dodo) Date: Thu Oct 22 14:31:16 2009 Subject: [Histonet] AFB Control Blocks Message-ID: Can anyone help me in getting some good AFB control blocks? I have Pneumocystis Controls? Blocks for trade. Help! _________________________________________________________________ Windows 7: It helps you do more. Explore Windows 7. http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen3:102009 From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Oct 22 14:30:32 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Oct 22 14:33:49 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: <650541.91710.qm@web113818.mail.gq1.yahoo.com> References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com>, <650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: I just want to know what CAP question states that you cannot have plants in the lab. Or is this the inspectors interpretation of a CAP question. Maybe the question regarding the lab working conditions. I could see if you have plants all over the counters crowding the space. But that is not regarding the plants themselves that would be in regard to having a crowded work environment. ?? When you give a phase 1 deficiency you have to reference the CAP question. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha [akemiat3377@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mahaynes <@t> cnmc.org Thu Oct 22 14:35:29 2009 From: mahaynes <@t> cnmc.org (Haynes, MaryAnne) Date: Thu Oct 22 14:35:35 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: <650541.91710.qm@web113818.mail.gq1.yahoo.com> References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> <650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. ?She stated that her lab was dinged for having plants in the lab by a CAP inspector. ? I had the same thing happen to me years ago. ?The inspector stated that plants attract insects that can contaminate a supposedly clean environment. ? Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. ?I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific.? The powers that be made me remove it from the lab for an inspection.? It went to live in one of the administrator's office for several months.? And died!? I think it needed the fumes!? Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive.? The absorption of Fume Matter is a secondary, but beneficial, effect.? You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ploykasek <@t> phenopath.com Thu Oct 22 14:44:30 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Oct 22 14:44:40 2009 Subject: [Histonet] Plants in lab Message-ID: Hi All. Just to clarify - the inspection we had was not a CAP inspection. It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From joseph-galbraith <@t> uiowa.edu Thu Oct 22 14:54:45 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Thu Oct 22 14:54:55 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com>, <650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: All: We had an inspector comment that plants were discouraged only because of the possibility that the plant pollens or the microbes on the plants or in the soil could end up on the water bath (and other work surfaces) and hence ultimately (directly or indirectly) in the tissue on the slides where the pathologist may have to determine if they were contaminants or integral to the tissue. Most of the time, this would be trivial but in some cases the issue could be substantive. So we were told. Sadly, we no longer have plants in the lab despite their positive impact on air quality and employee satisfaction. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 22, 2009 2:31 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT I just want to know what CAP question states that you cannot have plants in the lab. Or is this the inspectors interpretation of a CAP question. Maybe the question regarding the lab working conditions. I could see if you have plants all over the counters crowding the space. But that is not regarding the plants themselves that would be in regard to having a crowded work environment. ?? When you give a phase 1 deficiency you have to reference the CAP question. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha [akemiat3377@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Oct 22 14:59:25 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Oct 22 14:59:31 2009 Subject: [Histonet] Plants in lab Message-ID: <609450.12568.qm@web113802.mail.gq1.yahoo.com> Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab.?Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill.. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All.? Just to clarify - the inspection we had was not a CAP inspection.. It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Oct 22 15:30:21 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Oct 22 15:30:28 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: Message-ID: All, We as human beings are sloughing off skin cells, germs, hair, etc... Since we could contaminate the lab, I move that all people be banned from the histo lab...oh yeah, how would the work get done? Long story short, it is correct that plants can cause contamination, but it is overkill to ban them from the lab since almost anything could be considered a possible contaminant. All things that may give lab workers joy, peace or general enjoyment need not be banned. Maybe in a couple years, the No Fun No Happiness agenda can be put in place, but for now I will enjoy looking at our plants. Cheers, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Thursday, October 22, 2009 2:55 PM To: McMahon, Loralee A; Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT All: We had an inspector comment that plants were discouraged only because of the possibility that the plant pollens or the microbes on the plants or in the soil could end up on the water bath (and other work surfaces) and hence ultimately (directly or indirectly) in the tissue on the slides where the pathologist may have to determine if they were contaminants or integral to the tissue. Most of the time, this would be trivial but in some cases the issue could be substantive. So we were told. Sadly, we no longer have plants in the lab despite their positive impact on air quality and employee satisfaction. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 22, 2009 2:31 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT I just want to know what CAP question states that you cannot have plants in the lab. Or is this the inspectors interpretation of a CAP question. Maybe the question regarding the lab working conditions. I could see if you have plants all over the counters crowding the space. But that is not regarding the plants themselves that would be in regard to having a crowded work environment. ?? When you give a phase 1 deficiency you have to reference the CAP question. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha [akemiat3377@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Oct 22 15:38:48 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 22 15:34:05 2009 Subject: [Histonet] Plants in lab In-Reply-To: <609450.12568.qm@web113802.mail.gq1.yahoo.com> References: <609450.12568.qm@web113802.mail.gq1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A8698F@IBMB7Exchange.digestivespecialists.com> Windows!!!!!! Yahooooo If I had windows I wouldn't mind not having plants! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 3:59 PM To: histonet; Patti Loykasek Subject: Re: [Histonet] Plants in lab Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab.?Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill.. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All.? Just to clarify - the inspection we had was not a CAP inspection.. It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Oct 22 16:16:02 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Oct 22 16:20:57 2009 Subject: [Histonet] Plants & windows in lab In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390987A8698F@IBMB7Exchange.digestivespecialists.com> Message-ID: <141275.71183.qm@web113804.mail.gq1.yahoo.com> Linda, Patti's lab would make you envious indeed! ?Not only does she have windows, but she has windows lining the whole length of the lab looking onto a canal that has sailboats, as well as luxury ships passing by! ?The break room has the same view. ?The architect wanted the break-room to be offices, but Dr. Gown thought his staff needed a space to enjoy when they were taking a break. ?WOW a pathologist that thinks of his staff. ?That's a novel concept! Dr. Gown provides a?professional?Starbucks coffee maker that brews coffee to order, as well as provides Stash teas. ?He also has fresh organic fruit brought in every Monday for the staff, and stocks the frig with condiments. ?Unfortunately, most of the young people who have never had to pitch money in the coffee fund don't appreciate these bonuses. ?PhenoPath is a great place to work. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: RE: [Histonet] Plants in lab To: "'Akemi Allison-Tacha'" , "histonet" , "Patti Loykasek" Date: Thursday, October 22, 2009, 1:38 PM Windows!!!!!!? Yahooooo? If I had windows I wouldn't mind not having plants! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 3:59 PM To: histonet; Patti Loykasek Subject: Re: [Histonet] Plants in lab Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab.?Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill... Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All.? Just to clarify - the inspection we had was not a CAP inspection... It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A... at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Oct 22 21:46:26 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Oct 22 21:47:27 2009 Subject: [Histonet] Inspection question/OT References: <90354A475B420441B2A0396E5008D4967319D8@copc-sbs.COPC.local> <7722595275A4DD4FA225B92CDBF174A18C865A1216@EXC-MBX3.cfs.le.ac.uk> Message-ID: And don't forget a colony of cochineal beetles... Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 10/22/2009 9:14 AM To: 'Thomas Jasper'; Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inspection question/OT Plants grown in the lab might include, blue lungwort, dead man's fingers, mistletoe, cancer weed, devil's gut, bonewort, kidney vetch, liverwort, Hawaii birdnest spleenwort and if you have the space how about a Haematoxylum campechianum?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 21 October 2009 23:29 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inspection question Furthermore, most any plant (unless I'm missing something here) is beneficial for air quality...plants want CO2 and we appreciate their oxygen. I sure hope some inspector somewhere hasn't taken issue with plants your in the lab. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 21, 2009 3:17 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Inspection question No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Oct 22 21:54:58 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Oct 22 21:57:18 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com><650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: Sadly, our lab janitor could probably qualify as the same thing... And he's supposed to CLEAN the lab! Psoriasis skin flakes in a derm lab. Ewww. Sigh :( Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Haynes, MaryAnne Sent: Thu 10/22/2009 2:35 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Oct 22 22:02:28 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Oct 22 22:05:43 2009 Subject: [Histonet] Plants & windows in lab References: <141275.71183.qm@web113804.mail.gq1.yahoo.com> Message-ID: #1- Any openings? 2#- I think the ships would depress me a bit as I would constantly be reminded that I am not ON said luxury ship. 3#- Still couldn't give up my wonderful career though. Claire Linda, Patti's lab would make you envious indeed! Not only does she have windows, but she has windows lining the whole length of the lab looking onto a canal that has sailboats, as well as luxury ships passing by! The break room has the same view. The architect wanted the break-room to be offices, but Dr. Gown thought his staff needed a space to enjoy when they were taking a break. WOW a pathologist that thinks of his staff. That's a novel concept! Dr. Gown provides a professional Starbucks coffee maker that brews coffee to order, as well as provides Stash teas. He also has fresh organic fruit brought in every Monday for the staff, and stocks the frig with condiments. Unfortunately, most of the young people who have never had to pitch money in the coffee fund don't appreciate these bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, HT(ASCP)HTL From BMolinari <@t> heart.thi.tmc.edu Fri Oct 23 05:48:34 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Oct 23 05:48:38 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com><650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: This had nothing to do with an inspection, but follows this comment. There were 3 plants in our lab and there was one histotech who starting sneezing and got congested whenever he sat at his microtome. The plants were in front of him on a raised shelf. Finally we had a thought it may be the plants so we took them down and had a closer look and indeed there was a moldy material growing in the soil. Removed the plants and it was resolved. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Haynes, MaryAnne Sent: Thursday, October 22, 2009 2:35 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. ?She stated that her lab was dinged for having plants in the lab by a CAP inspector. ? I had the same thing happen to me years ago. ?The inspector stated that plants attract insects that can contaminate a supposedly clean environment. ? Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. ?I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific.? The powers that be made me remove it from the lab for an inspection.? It went to live in one of the administrator's office for several months.? And died!? I think it needed the fumes!? Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive.? The absorption of Fume Matter is a secondary, but beneficial, effect.? You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Oct 23 07:00:06 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Oct 23 07:00:19 2009 Subject: [Histonet] What's a "Window"? OT Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BBC@nmdamailsvr.nmda.ad.nmsu.edu> Not only will I not have a window in my new lab, but there's no chance of piping in sunlight to where I'll be! However, I have moved up one floor and will no longer be in the basement and my new lab is gorgeous and roomy and has actual air quality capability! A step up for histology person-kind! Send spider plant babies! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From leswes <@t> shaw.ca Fri Oct 23 08:34:59 2009 From: leswes <@t> shaw.ca (Lesley Weston) Date: Fri Oct 23 08:35:51 2009 Subject: [Histonet] RE:Missing the point of Plants-in-the-lab OT In-Reply-To: References: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> <650541.91710.qm@web113818.mail.gq1.yahoo.com> Message-ID: <830D6476-8373-49A6-81E9-BA6DB8E927CF@shaw.ca> People have much the same effect. Lesley Weston. On 22-Oct-09, at 12:35 PM, Haynes, MaryAnne wrote: > Some Health departments state that plants and their potting soil > can be a potential microbial and fungi contaminate in the lab. > Mary Anne Haynes > > Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) > Pathology Manager > Children's National Medical Center > Department of Anatomic Pathology > 111 Michigan Ave NW > Washington, DC 20010 > 202-476-4311 (office) > 202 476-4030 (fax) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha > Sent: Thursday, October 22, 2009 15:26 > To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; > Patti Loykasek > Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT > > Hi All, > I think all of you are missing the point of Patti's question. She > stated that her lab was dinged for having plants in the lab by a > CAP inspector. > I had the same thing happen to me years ago. The inspector stated > that plants attract insects that can contaminate a supposedly clean > environment. > Patti has an extremely well organized lab that only had a small > phase (1) deficiency last year. I think the inspector couldn't > find anything, so they had to come up with this ridiculous infraction. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > PresidentPhoenix Lab ConsultingTele: 408.402.5257 > Cell: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > > > --- On Thu, 10/22/09, Blazek, Linda > wrote: > > From: Blazek, Linda > Subject: [Histonet] RE: Plants-in-the-lab OT > To: "'Breeden, Sara'" , > "histonet@lists.utsouthwestern.edu" > > Date: Thursday, October 22, 2009, 12:15 PM > > I don't know Sally, but where I worked many moons ago I had a > spider plant that was extremely prolific. The powers that be made > me remove it from the lab for an inspection. It went to live in > one of the administrator's office for several months. And died! I > think it needed the fumes! Or it missed me. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, October 22, 2009 3:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Plants-in-the-lab OT > > I think it's the fluorescent lights that makes them thrive. The > absorption of Fume Matter is a secondary, but beneficial, effect. You > go, chlorophyll! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended > recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure or > distribution is prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Fri Oct 23 08:36:22 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Oct 23 08:35:55 2009 Subject: [Histonet] Plants & windows in lab In-Reply-To: <141275.71183.qm@web113804.mail.gq1.yahoo.com> Message-ID: What a great guy Dr. Gown is!! Kudos, Dr. Gown. I'm printing this email and leaving it in strategic places in the lab! Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Akemi Allison-Tacha Sent by: histonet-bounces@lists.utsouthwestern.edu 10/22/2009 05:22 PM To histonet , Patti Loykasek , LindaBlazek cc Subject RE: [Histonet] Plants & windows in lab Linda, Patti's lab would make you envious indeed! Not only does she have windows, but she has windows lining the whole length of the lab looking onto a canal that has sailboats, as well as luxury ships passing by! The break room has the same view. The architect wanted the break-room to be offices, but Dr. Gown thought his staff needed a space to enjoy when they were taking a break. WOW a pathologist that thinks of his staff. That's a novel concept! Dr. Gown provides a professional Starbucks coffee maker that brews coffee to order, as well as provides Stash teas. He also has fresh organic fruit brought in every Monday for the staff, and stocks the frig with condiments. Unfortunately, most of the young people who have never had to pitch money in the coffee fund don't appreciate these bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: RE: [Histonet] Plants in lab To: "'Akemi Allison-Tacha'" , "histonet" , "Patti Loykasek" Date: Thursday, October 22, 2009, 1:38 PM Windows!!!!!! Yahooooo If I had windows I wouldn't mind not having plants! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 3:59 PM To: histonet; Patti Loykasek Subject: Re: [Histonet] Plants in lab Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill... Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All. Just to clarify - the inspection we had was not a CAP inspection... It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A... at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From WBENTON <@t> umm.edu Fri Oct 23 08:57:09 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Fri Oct 23 08:57:39 2009 Subject: [Histonet] Re: Histonet Digest, Vol 71, Issue 25 In-Reply-To: <61380EA4.916@GWIA2.umm.edu> References: <61380EA4.916@GWIA2.umm.edu> Message-ID: <4AE17DF4.D886.00F4.3@umm.edu> In response to the Tau antibody I have an antibody and protocol that works well if you are interested, but the antibody is not from Biocare. Message: 10 Date: Thu, 22 Oct 2009 14:56:53 -0400 From: "Angela Bitting" Subject: [Histonet] anti-Tau on BenchmarkXT To: "histonet" Message-ID: <4AE072B6.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset=US-ASCII I may have asked this before, but is anyone using BioCare's Tau antibody on their BenchmarkXT?? If so, would you be willing to share your experience with it? Thanks, Angie Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From leiker <@t> buffalo.edu Fri Oct 23 08:59:47 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Oct 23 08:59:50 2009 Subject: [Histonet] Plants & windows in lab In-Reply-To: References: <141275.71183.qm@web113804.mail.gq1.yahoo.com> Message-ID: <6285C87ACADEB63D4303D974@CDYwxp1931.ad.med.buffalo.edu> Yes that's exactly what I was going to say - I'd like to toss in my resume, please! :-) --On Thursday, October 22, 2009 10:02 PM -0500 Ingles Claire wrote: ># 1- Any openings? > 2#- I think the ships would depress me a bit as I would constantly be > reminded that I am not ON said luxury ship. 3#- Still couldn't give up my > wonderful career though. > Claire > > > > Linda, > Patti's lab would make you envious indeed! Not only does she have > windows, but she has windows lining the whole length of the lab looking > onto a canal that has sailboats, as well as luxury ships passing by! The > break room has the same view. The architect wanted the break-room to be > offices, but Dr. Gown thought his staff needed a space to enjoy when they > were taking a break. WOW a pathologist that thinks of his staff. That's > a novel concept! Dr. Gown provides a professional Starbucks coffee maker > that brews coffee to order, as well as provides Stash teas. He also has > fresh organic fruit brought in every Monday for the staff, and stocks the > frig with condiments. Unfortunately, most of the young people who have > never had to pitch money in the coffee fund don't appreciate these > bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, > HT(ASCP)HTL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From estellamireles <@t> gmail.com Fri Oct 23 09:11:16 2009 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Fri Oct 23 09:11:23 2009 Subject: [Histonet] Floaters in Waterbath Message-ID: I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks From DKBoyd <@t> chs.net Fri Oct 23 09:18:17 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Oct 23 09:17:37 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: Message-ID: Kim Wipes pulled across the top of the water will pick up most, if not all floaters. Very thin so they don't deplete the water bath. Should be done after each block to prevent floaters. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Stella Mireles Sent by: histonet-bounces@lists.utsouthwestern.edu 10/23/2009 10:12 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Jackie.O'Connor <@t> abbott.com Fri Oct 23 09:23:23 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Oct 23 09:23:52 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: References: Message-ID: Kim wipes seem to pick up more debris than paper towels, and they pick up much less water. We routinely sweep the waterbath with a kimwipe after each block. You can also pick up floaters from embedding if the forceps are not cleaned between each block. Most embedding centers have multiple wells for forceps - how often do you clean those wells? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-bounces@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Oct 23 09:31:34 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Oct 23 09:31:39 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C63052EF@EXC-MBX3.cfs.le.ac.uk> Hair net and gloves?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: 23 October 2009 15:11 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Oct 23 09:40:58 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Oct 23 09:41:10 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: References: Message-ID: <4AE18838.2B7F.00C9.0@geisinger.edu> We currently have a Quality Improvement Plan in effect to address this issue. Jackie is right about keeping those forcep wells clean. Although we don't swipe Kimwipes over our waterbath after each block, we do it very regularly. Another thing to consider is how often you clean your embedding molds. ~Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 ! >>> "Jackie M O'Connor" 10/23/2009 10:23 AM >>> Kim wipes seem to pick up more debris than paper towels, and they pick up much less water. We routinely sweep the waterbath with a kimwipe after each block. You can also pick up floaters from embedding if the forceps are not cleaned between each block. Most embedding centers have multiple wells for forceps - how often do you clean those wells? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-bounces@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From ttruscot <@t> vetmed.wsu.edu Fri Oct 23 09:43:07 2009 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Oct 23 09:43:11 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: References: Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4AF6A42CD88@CVMMBX.vetmed.wsu.edu> Hi Stella, Not only wiping the top of the waterbath water with kimwipes between each block, and keeping forceps clean at embedding, and keeping your slides clean, but also keeping things clean at grossing: clean cutting board and instruments between tissues or cases. One pathologist called it forcep metastasis. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 7:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCazares <@t> schosp.org Fri Oct 23 09:45:22 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Fri Oct 23 09:46:28 2009 Subject: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject In-Reply-To: References: Message-ID: <572D1F45B44B3D4096D554B4CB40639C01F226D9E3@EXCHCCRMB.schosp.org> Kim wipes work great, and if done after each block shouldn't slow things down much. Besides, what's the point of quantity when the quality is compromised with floaters. You should have a policy regarding this since it is a CAP requirement. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 9:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet estern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From Laura.Miller <@t> leica-microsystems.com Fri Oct 23 10:01:13 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Fri Oct 23 10:01:20 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 10/22/2009 and will not return until 10/26/2009. IF YOU NEED IMMEDIATE ASSISANCE PLEASE CONTACT ANDREAS KAEPPLEIN. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From settembr <@t> umdnj.edu Fri Oct 23 10:11:18 2009 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Oct 23 10:12:03 2009 Subject: [Histonet] Plants & windows in lab Message-ID: Where is PhenoPath? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Merced M Leiker 10/23/09 9:59 AM >>> Yes that's exactly what I was going to say - I'd like to toss in my resume, please! :-) --On Thursday, October 22, 2009 10:02 PM -0500 Ingles Claire wrote: ># 1- Any openings? > 2#- I think the ships would depress me a bit as I would constantly be > reminded that I am not ON said luxury ship. 3#- Still couldn't give up my > wonderful career though. > Claire > > > > Linda, > Patti's lab would make you envious indeed! Not only does she have > windows, but she has windows lining the whole length of the lab looking > onto a canal that has sailboats, as well as luxury ships passing by! The > break room has the same view. The architect wanted the break-room to be > offices, but Dr. Gown thought his staff needed a space to enjoy when they > were taking a break. WOW a pathologist that thinks of his staff. That's > a novel concept! Dr. Gown provides a professional Starbucks coffee maker > that brews coffee to order, as well as provides Stash teas. He also has > fresh organic fruit brought in every Monday for the staff, and stocks the > frig with condiments. Unfortunately, most of the young people who have > never had to pitch money in the coffee fund don't appreciate these > bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, > HT(ASCP)HTL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Fri Oct 23 10:21:34 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Oct 23 10:21:41 2009 Subject: [Histonet] Plants & windows in lab In-Reply-To: References: Message-ID: <4AE1C9FE.3040804@pathology.washington.edu> Seattle, WA Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Dana Settembre wrote: > Where is PhenoPath? > > > Dana Settembre, HT ASCP > Immunohistochemistry Lab > UMDNJ - University Hospital > Newark, NJ USA > > > >>>> Merced M Leiker 10/23/09 9:59 AM >>> >>>> > Yes that's exactly what I was going to say - I'd like to toss in my > resume, > please! :-) > > --On Thursday, October 22, 2009 10:02 PM -0500 Ingles Claire > wrote: > > >> # 1- Any openings? >> 2#- I think the ships would depress me a bit as I would constantly >> > be > >> reminded that I am not ON said luxury ship. 3#- Still couldn't give >> > up my > >> wonderful career though. >> Claire >> >> >> >> Linda, >> Patti's lab would make you envious indeed! Not only does she have >> windows, but she has windows lining the whole length of the lab >> > looking > >> onto a canal that has sailboats, as well as luxury ships passing by! >> > The > >> break room has the same view. The architect wanted the break-room to >> > be > >> offices, but Dr. Gown thought his staff needed a space to enjoy when >> > they > >> were taking a break. WOW a pathologist that thinks of his staff. >> > That's > >> a novel concept! Dr. Gown provides a professional Starbucks coffee >> > maker > >> that brews coffee to order, as well as provides Stash teas. He also >> > has > >> fresh organic fruit brought in every Monday for the staff, and stocks >> > the > >> frig with condiments. Unfortunately, most of the young people who >> > have > >> never had to pitch money in the coffee fund don't appreciate these >> bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha >> > BS, > >> HT(ASCP)HTL >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From NMargaryan <@t> childrensmemorial.org Fri Oct 23 10:30:59 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Oct 23 10:33:17 2009 Subject: [Histonet] human macrophages in human melanoma xenografts Message-ID: Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira From lblazek <@t> digestivespecialists.com Fri Oct 23 10:39:40 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 23 10:34:57 2009 Subject: [Histonet] Plants & windows in lab In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A86997@IBMB7Exchange.digestivespecialists.com> Don't go to the web site. It has a picture of the water and boats! It will just drive you batty! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Friday, October 23, 2009 11:11 AM To: Merced M Leiker; histonet; Ingles Claire Subject: RE: [Histonet] Plants & windows in lab Where is PhenoPath? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Merced M Leiker 10/23/09 9:59 AM >>> Yes that's exactly what I was going to say - I'd like to toss in my resume, please! :-) --On Thursday, October 22, 2009 10:02 PM -0500 Ingles Claire wrote: ># 1- Any openings? > 2#- I think the ships would depress me a bit as I would constantly be > reminded that I am not ON said luxury ship. 3#- Still couldn't give up my > wonderful career though. > Claire > > > > Linda, > Patti's lab would make you envious indeed! Not only does she have > windows, but she has windows lining the whole length of the lab looking > onto a canal that has sailboats, as well as luxury ships passing by! The > break room has the same view. The architect wanted the break-room to be > offices, but Dr. Gown thought his staff needed a space to enjoy when they > were taking a break. WOW a pathologist that thinks of his staff. That's > a novel concept! Dr. Gown provides a professional Starbucks coffee maker > that brews coffee to order, as well as provides Stash teas. He also has > fresh organic fruit brought in every Monday for the staff, and stocks the > frig with condiments. Unfortunately, most of the young people who have > never had to pitch money in the coffee fund don't appreciate these > bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, > HT(ASCP)HTL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Fri Oct 23 10:43:38 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Oct 23 10:43:49 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: References: Message-ID: Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Oct 23 10:50:32 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 23 10:50:37 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: Message-ID: F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Fri Oct 23 10:56:48 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Oct 23 10:59:02 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: References: Message-ID: Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Fri Oct 23 10:59:15 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Oct 23 10:59:20 2009 Subject: [Histonet] AFB Control Blocks In-Reply-To: References: Message-ID: Concerning Control Tissue Blocks: The NSH provides and Share and Exchange Service for members and non-members alike. Anytime you have a need for a particular type of control, please contact the NSH office (nsh.org) and we will try and meet your needs. The program is only intended for individual lab use and not commercial use (selling control slides). If you have any questions you can direct them to me and I will assist in any way I can. William DeSalvo, B.S., HTL(ASCP) Chair, NSH Quality Control Committee > From: jackdodo@msn.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 22 Oct 2009 15:31:10 -0400 > Subject: [Histonet] AFB Control Blocks > > > Can anyone help me in getting some good AFB control blocks? I have Pneumocystis Controls? Blocks for trade. Help! > _________________________________________________________________ > Windows 7: It helps you do more. Explore Windows 7. > http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen3:102009 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows 7: It works the way you want. Learn more. http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen2:102009 From liz <@t> premierlab.com Fri Oct 23 11:00:17 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 23 11:00:22 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: Message-ID: That will work but I'm not aware of the non-mouse anti-human macrophage marker, you could check the web or biocompare. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan@childrensmemorial.org] Sent: Friday, October 23, 2009 9:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Fri Oct 23 11:05:28 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Oct 23 11:05:32 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: References: Message-ID: Liz: Thanks for clarifying. Your comments are spot on. That's what I get for responding too quickly and not thinking things through. I would agree with Naira that red detection is useful in the melanoma context. Joe 380 MRC 4-4737 (voice) 3-3482 (fax) pager 131-1170 joseph-galbraith@uiowa.edu -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 11:00 AM To: Margaryan, Naira; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts That will work but I'm not aware of the non-mouse anti-human macrophage marker, you could check the web or biocompare. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan@childrensmemorial.org] Sent: Friday, October 23, 2009 9:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Fri Oct 23 11:42:30 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Oct 23 11:42:40 2009 Subject: [Histonet] Collagen staining using special stains, lighter vs darker Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A138E5121@MD1EV002.medimmune.com> I have an investigator looking at Massons(with Aniline Blue), Van Gieson and Gomori's all showing the same type of collagen staining. In some areas collagen fibers are brilliantly stained and other fibers are pale all on the same slide. This is consistent across the board so we know it is not the stain, rather the tissue response. I want to say I have heard lighter collagen fibers are either immature or dying. I mean that is fine, but does anyone have any real answers? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Jackie.O'Connor <@t> abbott.com Fri Oct 23 11:47:21 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Oct 23 11:47:49 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: References: Message-ID: Naira - These are presumably SCID mice? You can use a mouse mAb with no Ig interference, since SCIDs do not have an immune system. You can get the rare 'leaker', but I've used murine mAb routinely for human xenografts in SCIDs. Now, Nudes are a different story. From: "Margaryan, Naira" To: Liz Chlipala , "Galbraith, Joe" , "histonet@lists.utsouthwestern.edu" Date: 10/23/2009 10:59 AM Subject: RE: [Histonet] human macrophages in human melanoma xenografts Sent by: histonet-bounces@lists.utsouthwestern.edu Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmarti <@t> cmrb.eu Fri Oct 23 11:48:50 2009 From: mmarti <@t> cmrb.eu (Marti Gaudes, Merce) Date: Fri Oct 23 11:53:43 2009 Subject: [Histonet] gap junction by electron microscopy Message-ID: Hi all. I need to study gap junctions in heart tissue by electron microscopy. Does anyone know if I need some specific staining to see the gap junctions? Thanks a lot. Merc? Mart? Gaudes -------- Abans d'imprimir aquest missatge, si us plau, comprova que ??s realment necessari. El medi ambient ??s cosa de tots. Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. -------- La informaci?? continguda en aquest missatge i en qualsevol fitxer adjunt ??s confidencial, privada i d'??s exclusiu per al destinatari. Si no ??s la persona a la qual anava dirigida aquesta informaci??, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c??pia, divulgaci??, distribuci?? o utilitzaci?? no autoritzada d'aquest correu electr??nic i dels seus adjunts est?? prohibida en virtut de la legislaci?? vigent. La informaci??n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci??n, por favor, notifique inmediatamente el env??o err??neo al remitente y borre el mensaje. Cualquier copia, divulgaci??n, distribuci??n o utilizaci??n no autorizada de este correo electr??nico y de sus adjuntos est?? prohibida en virtud de la legislaci??n vigente. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. -------- From mcauliff <@t> umdnj.edu Fri Oct 23 12:01:58 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Oct 23 12:00:11 2009 Subject: [Histonet] Collagen staining using special stains, lighter vs darker In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A138E5121@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A138E5121@MD1EV002.medimmune.com> Message-ID: <4AE1E186.3050801@umdnj.edu> The first things to check are 1. adequate fixation. 2. Complete removal of paraffin. That done, I don't think you can use staining intensity to draw any conclusions about the health, or lack thereof, of collagen. I suggest finding some references on collagen production and degradation before proceeding. Geoff Madary, Joseph wrote: > I have an investigator looking at Massons(with Aniline Blue), Van Gieson > and Gomori's all showing the same type of collagen staining. In some > areas collagen fibers are brilliantly stained and other fibers are pale > all on the same slide. This is consistent across the board so we know > it is not the stain, rather the tissue response. I want to say I have > heard lighter collagen fibers are either immature or dying. I mean that > is fine, but does anyone have any real answers? > > > > Nick Madary, HT/HTL(ASCP)QIHC > > Histology Mgr, Medimmune > > 301.398.6360(lab), 4745(vm),9745(fax) > > > > "To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary, and > expected to be used only by the individual(s) for whom it is intended. > If you have received this electronic communication in error, please > reply to the sender advising of the error in transmission and delete the > original message and any accompanying documents from your system > immediately, without copying, reviewing or otherwise using them for any > purpose. Thank you for your cooperation." > > > > > > > > > To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From MadaryJ <@t> MedImmune.com Fri Oct 23 12:04:41 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Oct 23 12:04:52 2009 Subject: [Histonet] IHC fibrin antibody for mouse tissue? Suggestions? In-Reply-To: References: Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A138E5145@MD1EV002.medimmune.com> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, October 23, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 71, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: RE:Missing the point of Plants-in-the-lab OT (Dawson, Glen) 2. RE: Plants in lab (Blazek, Linda) 3. RE: Plants & windows in lab (Akemi Allison-Tacha) 4. RE: Inspection question/OT (Ingles Claire ) 5. RE: RE:Missing the point of Plants-in-the-lab OT (Ingles Claire ) 6. RE: Plants & windows in lab (Ingles Claire ) 7. RE: RE:Missing the point of Plants-in-the-lab OT (Molinari, Betsy) 8. What's a "Window"? OT (Breeden, Sara) 9. Re: RE:Missing the point of Plants-in-the-lab OT (Lesley Weston) 10. RE: Plants & windows in lab (DKBoyd@chs.net) 11. Re: Histonet Digest, Vol 71, Issue 25 (Walter Benton) 12. RE: Plants & windows in lab (Merced M Leiker) 13. Floaters in Waterbath (Stella Mireles) 14. Re: Floaters in Waterbath (DKBoyd@chs.net) 15. Re: Floaters in Waterbath (Jackie M O'Connor) 16. RE: Floaters in Waterbath (Edwards, R.E.) 17. Re: Floaters in Waterbath (Angela Bitting) 18. RE: Floaters in Waterbath (Truscott, Tom) 19. RE: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject (Cazares, Ruth) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 Oct 2009 15:30:21 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, We as human beings are sloughing off skin cells, germs, hair, etc... Since we could contaminate the lab, I move that all people be banned from the histo lab...oh yeah, how would the work get done? Long story short, it is correct that plants can cause contamination, but it is overkill to ban them from the lab since almost anything could be considered a possible contaminant. All things that may give lab workers joy, peace or general enjoyment need not be banned. Maybe in a couple years, the No Fun No Happiness agenda can be put in place, but for now I will enjoy looking at our plants. Cheers, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Thursday, October 22, 2009 2:55 PM To: McMahon, Loralee A; Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT All: We had an inspector comment that plants were discouraged only because of the possibility that the plant pollens or the microbes on the plants or in the soil could end up on the water bath (and other work surfaces) and hence ultimately (directly or indirectly) in the tissue on the slides where the pathologist may have to determine if they were contaminants or integral to the tissue. Most of the time, this would be trivial but in some cases the issue could be substantive. So we were told. Sadly, we no longer have plants in the lab despite their positive impact on air quality and employee satisfaction. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 22, 2009 2:31 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT I just want to know what CAP question states that you cannot have plants in the lab. Or is this the inspectors interpretation of a CAP question. Maybe the question regarding the lab working conditions. I could see if you have plants all over the counters crowding the space. But that is not regarding the plants themselves that would be in regard to having a crowded work environment. ?? When you give a phase 1 deficiency you have to reference the CAP question. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha [akemiat3377@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 22 Oct 2009 16:38:48 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Plants in lab To: 'Akemi Allison-Tacha' , histonet , Patti Loykasek Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A8698F@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="iso-8859-1" Windows!!!!!! Yahooooo If I had windows I wouldn't mind not having plants! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 3:59 PM To: histonet; Patti Loykasek Subject: Re: [Histonet] Plants in lab Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab.?Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill.. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All.? Just to clarify - the inspection we had was not a CAP inspection.. It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 22 Oct 2009 14:16:02 -0700 (PDT) From: Akemi Allison-Tacha Subject: RE: [Histonet] Plants & windows in lab To: histonet , Patti Loykasek , LindaBlazek Message-ID: <141275.71183.qm@web113804.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Linda, Patti's lab would make you envious indeed! ?Not only does she have windows, but she has windows lining the whole length of the lab looking onto a canal that has sailboats, as well as luxury ships passing by! ?The break room has the same view. ?The architect wanted the break-room to be offices, but Dr. Gown thought his staff needed a space to enjoy when they were taking a break. ?WOW a pathologist that thinks of his staff. ?That's a novel concept! Dr. Gown provides a?professional?Starbucks coffee maker that brews coffee to order, as well as provides Stash teas. ?He also has fresh organic fruit brought in every Monday for the staff, and stocks the frig with condiments. ?Unfortunately, most of the young people who have never had to pitch money in the coffee fund don't appreciate these bonuses. ?PhenoPath is a great place to work. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: RE: [Histonet] Plants in lab To: "'Akemi Allison-Tacha'" , "histonet" , "Patti Loykasek" Date: Thursday, October 22, 2009, 1:38 PM Windows!!!!!!? Yahooooo? If I had windows I wouldn't mind not having plants! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 3:59 PM To: histonet; Patti Loykasek Subject: Re: [Histonet] Plants in lab Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab.?Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill... Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All.? Just to clarify - the inspection we had was not a CAP inspection... It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A... at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 22 Oct 2009 21:46:26 -0500 From: "Ingles Claire " Subject: RE: [Histonet] Inspection question/OT To: "Edwards, R.E." , "Thomas Jasper" , "Weems, Joyce" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" And don't forget a colony of cochineal beetles... Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 10/22/2009 9:14 AM To: 'Thomas Jasper'; Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inspection question/OT Plants grown in the lab might include, blue lungwort, dead man's fingers, mistletoe, cancer weed, devil's gut, bonewort, kidney vetch, liverwort, Hawaii birdnest spleenwort and if you have the space how about a Haematoxylum campechianum?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 21 October 2009 23:29 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Inspection question Furthermore, most any plant (unless I'm missing something here) is beneficial for air quality...plants want CO2 and we appreciate their oxygen. I sure hope some inspector somewhere hasn't taken issue with plants your in the lab. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 21, 2009 3:17 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Inspection question No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 21, 2009 18:15 To: histonet Subject: [Histonet] Inspection question Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard & must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 22 Oct 2009 21:54:58 -0500 From: "Ingles Claire " Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sadly, our lab janitor could probably qualify as the same thing... And he's supposed to CLEAN the lab! Psoriasis skin flakes in a derm lab. Ewww. Sigh :( Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Haynes, MaryAnne Sent: Thu 10/22/2009 2:35 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 22 Oct 2009 22:02:28 -0500 From: "Ingles Claire " Subject: RE: [Histonet] Plants & windows in lab To: "histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" #1- Any openings? 2#- I think the ships would depress me a bit as I would constantly be reminded that I am not ON said luxury ship. 3#- Still couldn't give up my wonderful career though. Claire Linda, Patti's lab would make you envious indeed! Not only does she have windows, but she has windows lining the whole length of the lab looking onto a canal that has sailboats, as well as luxury ships passing by! The break room has the same view. The architect wanted the break-room to be offices, but Dr. Gown thought his staff needed a space to enjoy when they were taking a break. WOW a pathologist that thinks of his staff. That's a novel concept! Dr. Gown provides a professional Starbucks coffee maker that brews coffee to order, as well as provides Stash teas. He also has fresh organic fruit brought in every Monday for the staff, and stocks the frig with condiments. Unfortunately, most of the young people who have never had to pitch money in the coffee fund don't appreciate these bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, HT(ASCP)HTL ------------------------------ Message: 7 Date: Fri, 23 Oct 2009 05:48:34 -0500 From: "Molinari, Betsy" Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" This had nothing to do with an inspection, but follows this comment. There were 3 plants in our lab and there was one histotech who starting sneezing and got congested whenever he sat at his microtome. The plants were in front of him on a raised shelf. Finally we had a thought it may be the plants so we took them down and had a closer look and indeed there was a moldy material growing in the soil. Removed the plants and it was resolved. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Haynes, MaryAnne Sent: Thursday, October 22, 2009 2:35 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. ?She stated that her lab was dinged for having plants in the lab by a CAP inspector. ? I had the same thing happen to me years ago. ?The inspector stated that plants attract insects that can contaminate a supposedly clean environment. ? Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. ?I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: [Histonet] RE: Plants-in-the-lab OT To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific.? The powers that be made me remove it from the lab for an inspection.? It went to live in one of the administrator's office for several months.? And died!? I think it needed the fumes!? Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive.? The absorption of Fume Matter is a secondary, but beneficial, effect.? You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 23 Oct 2009 06:00:06 -0600 From: "Breeden, Sara" Subject: [Histonet] What's a "Window"? OT To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BBC@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Not only will I not have a window in my new lab, but there's no chance of piping in sunlight to where I'll be! However, I have moved up one floor and will no longer be in the basement and my new lab is gorgeous and roomy and has actual air quality capability! A step up for histology person-kind! Send spider plant babies! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 9 Date: Fri, 23 Oct 2009 06:34:59 -0700 From: Lesley Weston Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT To: histonet@lists.utsouthwestern.edu Message-ID: <830D6476-8373-49A6-81E9-BA6DB8E927CF@shaw.ca> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed People have much the same effect. Lesley Weston. On 22-Oct-09, at 12:35 PM, Haynes, MaryAnne wrote: > Some Health departments state that plants and their potting soil > can be a potential microbial and fungi contaminate in the lab. > Mary Anne Haynes > > Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) > Pathology Manager > Children's National Medical Center > Department of Anatomic Pathology > 111 Michigan Ave NW > Washington, DC 20010 > 202-476-4311 (office) > 202 476-4030 (fax) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha > Sent: Thursday, October 22, 2009 15:26 > To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; > Patti Loykasek > Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT > > Hi All, > I think all of you are missing the point of Patti's question. She > stated that her lab was dinged for having plants in the lab by a > CAP inspector. > I had the same thing happen to me years ago. The inspector stated > that plants attract insects that can contaminate a supposedly clean > environment. > Patti has an extremely well organized lab that only had a small > phase (1) deficiency last year. I think the inspector couldn't > find anything, so they had to come up with this ridiculous infraction. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > PresidentPhoenix Lab ConsultingTele: 408.402.5257 > Cell: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > > > --- On Thu, 10/22/09, Blazek, Linda > wrote: > > From: Blazek, Linda > Subject: [Histonet] RE: Plants-in-the-lab OT > To: "'Breeden, Sara'" , > "histonet@lists.utsouthwestern.edu" > > Date: Thursday, October 22, 2009, 12:15 PM > > I don't know Sally, but where I worked many moons ago I had a > spider plant that was extremely prolific. The powers that be made > me remove it from the lab for an inspection. It went to live in > one of the administrator's office for several months. And died! I > think it needed the fumes! Or it missed me. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, October 22, 2009 3:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Plants-in-the-lab OT > > I think it's the fluorescent lights that makes them thrive. The > absorption of Fume Matter is a secondary, but beneficial, effect. You > go, chlorophyll! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended > recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure or > distribution is prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 23 Oct 2009 09:36:22 -0400 From: DKBoyd@chs.net Subject: RE: [Histonet] Plants & windows in lab To: Akemi Allison-Tacha Cc: histonet-bounces@lists.utsouthwestern.edu, histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" What a great guy Dr. Gown is!! Kudos, Dr. Gown. I'm printing this email and leaving it in strategic places in the lab! Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Akemi Allison-Tacha Sent by: histonet-bounces@lists.utsouthwestern.edu 10/22/2009 05:22 PM To histonet , Patti Loykasek , LindaBlazek cc Subject RE: [Histonet] Plants & windows in lab Linda, Patti's lab would make you envious indeed! Not only does she have windows, but she has windows lining the whole length of the lab looking onto a canal that has sailboats, as well as luxury ships passing by! The break room has the same view. The architect wanted the break-room to be offices, but Dr. Gown thought his staff needed a space to enjoy when they were taking a break. WOW a pathologist that thinks of his staff. That's a novel concept! Dr. Gown provides a professional Starbucks coffee maker that brews coffee to order, as well as provides Stash teas. He also has fresh organic fruit brought in every Monday for the staff, and stocks the frig with condiments. Unfortunately, most of the young people who have never had to pitch money in the coffee fund don't appreciate these bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Blazek, Linda wrote: From: Blazek, Linda Subject: RE: [Histonet] Plants in lab To: "'Akemi Allison-Tacha'" , "histonet" , "Patti Loykasek" Date: Thursday, October 22, 2009, 1:38 PM Windows!!!!!! Yahooooo If I had windows I wouldn't mind not having plants! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 3:59 PM To: histonet; Patti Loykasek Subject: Re: [Histonet] Plants in lab Well, that makes more sense, especially after Mary's statement "Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes" Patti, you do have a wonderful assortment of plants lining your window sill... Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com --- On Thu, 10/22/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Plants in lab To: "histonet" Date: Thursday, October 22, 2009, 12:44 PM Hi All. Just to clarify - the inspection we had was not a CAP inspection... It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A... at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 11 Date: Fri, 23 Oct 2009 09:57:09 -0400 From: "Walter Benton" Subject: [Histonet] Re: Histonet Digest, Vol 71, Issue 25 To: Message-ID: <4AE17DF4.D886.00F4.3@umm.edu> Content-Type: text/plain; charset=US-ASCII In response to the Tau antibody I have an antibody and protocol that works well if you are interested, but the antibody is not from Biocare. Message: 10 Date: Thu, 22 Oct 2009 14:56:53 -0400 From: "Angela Bitting" Subject: [Histonet] anti-Tau on BenchmarkXT To: "histonet" Message-ID: <4AE072B6.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset=US-ASCII I may have asked this before, but is anyone using BioCare's Tau antibody on their BenchmarkXT?? If so, would you be willing to share your experience with it? Thanks, Angie Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 12 Date: Fri, 23 Oct 2009 09:59:47 -0400 From: Merced M Leiker Subject: RE: [Histonet] Plants & windows in lab To: Ingles Claire , histonet Message-ID: <6285C87ACADEB63D4303D974@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Yes that's exactly what I was going to say - I'd like to toss in my resume, please! :-) --On Thursday, October 22, 2009 10:02 PM -0500 Ingles Claire wrote: ># 1- Any openings? > 2#- I think the ships would depress me a bit as I would constantly be > reminded that I am not ON said luxury ship. 3#- Still couldn't give up my > wonderful career though. > Claire > > > > Linda, > Patti's lab would make you envious indeed! Not only does she have > windows, but she has windows lining the whole length of the lab looking > onto a canal that has sailboats, as well as luxury ships passing by! The > break room has the same view. The architect wanted the break-room to be > offices, but Dr. Gown thought his staff needed a space to enjoy when they > were taking a break. WOW a pathologist that thinks of his staff. That's > a novel concept! Dr. Gown provides a professional Starbucks coffee maker > that brews coffee to order, as well as provides Stash teas. He also has > fresh organic fruit brought in every Monday for the staff, and stocks the > frig with condiments. Unfortunately, most of the young people who have > never had to pitch money in the coffee fund don't appreciate these > bonuses. PhenoPath is a great place to work. Akemi Allison-Tacha BS, > HT(ASCP)HTL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 13 Date: Fri, 23 Oct 2009 09:11:16 -0500 From: Stella Mireles Subject: [Histonet] Floaters in Waterbath To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks ------------------------------ Message: 14 Date: Fri, 23 Oct 2009 10:18:17 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] Floaters in Waterbath To: Stella Mireles Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Kim Wipes pulled across the top of the water will pick up most, if not all floaters. Very thin so they don't deplete the water bath. Should be done after each block to prevent floaters. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Stella Mireles Sent by: histonet-bounces@lists.utsouthwestern.edu 10/23/2009 10:12 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 15 Date: Fri, 23 Oct 2009 09:23:23 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] Floaters in Waterbath To: Stella Mireles Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Kim wipes seem to pick up more debris than paper towels, and they pick up much less water. We routinely sweep the waterbath with a kimwipe after each block. You can also pick up floaters from embedding if the forceps are not cleaned between each block. Most embedding centers have multiple wells for forceps - how often do you clean those wells? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-bounces@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 23 Oct 2009 15:31:34 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Floaters in Waterbath To: 'Stella Mireles' , "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C63052EF@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Hair net and gloves?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: 23 October 2009 15:11 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 23 Oct 2009 10:40:58 -0400 From: "Angela Bitting" Subject: Re: [Histonet] Floaters in Waterbath To: "Jackie M O'Connor" , "Stella Mireles" , Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4AE18838.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" We currently have a Quality Improvement Plan in effect to address this issue. Jackie is right about keeping those forcep wells clean. Although we don't swipe Kimwipes over our waterbath after each block, we do it very regularly. Another thing to consider is how often you clean your embedding molds. ~Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 ! >>> "Jackie M O'Connor" 10/23/2009 10:23 AM >>> Kim wipes seem to pick up more debris than paper towels, and they pick up much less water. We routinely sweep the waterbath with a kimwipe after each block. You can also pick up floaters from embedding if the forceps are not cleaned between each block. Most embedding centers have multiple wells for forceps - how often do you clean those wells? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-bounces@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 18 Date: Fri, 23 Oct 2009 07:43:07 -0700 From: "Truscott, Tom" Subject: RE: [Histonet] Floaters in Waterbath To: Stella Mireles , "Histonet@lists.utsouthwestern.edu" Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4AF6A42CD88@CVMMBX.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Hi Stella, Not only wiping the top of the waterbath water with kimwipes between each block, and keeping forceps clean at embedding, and keeping your slides clean, but also keeping things clean at grossing: clean cutting board and instruments between tissues or cases. One pathologist called it forcep metastasis. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 7:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 23 Oct 2009 09:45:22 -0500 From: "Cazares, Ruth" Subject: RE: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject To: Stella Mireles , "Histonet@lists.utsouthwestern.edu" Message-ID: <572D1F45B44B3D4096D554B4CB40639C01F226D9E3@EXCHCCRMB.schosp.org> Content-Type: text/plain; charset="us-ascii" Kim wipes work great, and if done after each block shouldn't slow things down much. Besides, what's the point of quantity when the quality is compromised with floaters. You should have a policy regarding this since it is a CAP requirement. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 9:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet estern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 71, Issue 26 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From mcauliff <@t> umdnj.edu Fri Oct 23 12:14:08 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Oct 23 12:12:21 2009 Subject: [Histonet] gap junction by electron microscopy In-Reply-To: References: Message-ID: <4AE1E460.1070100@umdnj.edu> Tannic acid in the fix, both in the glut and the osmium if I remember correctly, will help but I don't think it is absolutely necessary. Check out any of the big cardiology texts, there will be a chapter or two on normal cardiac anatomy from the gross level down to EM level. Look at the EMs and check the references. Geoff Marti Gaudes, Merce wrote: > Hi all. > > I need to study gap junctions in heart tissue by electron microscopy. Does anyone know if I need some specific staining to see the gap junctions? > > Thanks a lot. > > > > > > Merc? Mart? Gaudes > > > > > > -------- > Abans d'imprimir aquest missatge, si us plau, comprova que ??s realment > necessari. El medi ambient ??s cosa de tots. > > Antes de imprimir este mensaje, por favor, comprueba que es realmente > necesario. El medio ambiente es cosa de todos. > > Before printing this e-mail, please make certain it is absolutely necessary. > The environment is everybody's business. > -------- > > La informaci?? continguda en aquest missatge i en qualsevol fitxer > adjunt ??s confidencial, privada i d'??s exclusiu per al destinatari. > Si no ??s la persona a la qual anava dirigida aquesta informaci??, si us > plau, notifiqui immediatament l'enviament erroni al remitent i esborri > el missatge. Qualsevol c??pia, divulgaci??, distribuci?? o utilitzaci?? no > autoritzada d'aquest correu electr??nic i dels seus adjunts est? > prohibida en virtut de la legislaci?? vigent. > > La informaci??n contenida en este mensaje y en cualquier fichero > adjunto es confidencial, privada y de uso exclusivo para el > destinatario. Si usted no es la persona a la cual iba dirigida esta > informaci??n, por favor, notifique inmediatamente el env??o err??neo al > remitente y borre el mensaje. Cualquier copia, divulgaci??n, > distribuci??n o utilizaci??n no autorizada de este correo electr??nico y > de sus adjuntos est?? prohibida en virtud de la legislaci??n vigente. > > The information included in this e-mail and any attached files is > confidential and private. If you are not the intended recipient, > please notify the sender and delete this message immediately. > Dissemination, forwarding or copying of this e-mail and > its associated attachments is strictly prohibited in accordance with > current legislation. > -------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From dmikita <@t> wmcnet.org Fri Oct 23 12:16:31 2009 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Fri Oct 23 12:16:39 2009 Subject: [Histonet] Formalin and Xylene Exposure Message-ID: <4AE19090.1875.003A.0@wmcnet.org> Hello, I was wondering what your guys protocol on working in the histology lab and gross room with formalin and xylene in use (without charcoal filtration), is when the ventilation (exhaust) system goes down. Do you have respirators to wear or do you evacuate everyone until the ventilation system is back up and running? Thanks, Daryl Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 Phone: 307-577-2198 Fax: 307-577-2731 From janderson <@t> halozyme.com Fri Oct 23 12:51:45 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Fri Oct 23 12:52:08 2009 Subject: [Histonet] Masson's tri-chrome specificity Message-ID: <5F7CC9B788911848A79BC83453A3D6530360CF3CC8@Tomlinson.hti.com> Good morning Histonet. Thanks a lot to so many people who helped me with my Leica XL question - your wealth of knowledge never ceases to impress me. I have a different question now about Masson's tri-chrome and it's specificity on isolated rabbit tendon. There is some muscle still attached to the ends of the tendon. We get both Beibrich Scarlet and Aniline Blue staining, at different intensities, within each collagen sample (even control). How can I explain this? Would there be a possibility to omit the Beibrich and stain with eosin and Aniline blue? Thanks so much! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From tjasper <@t> copc.net Fri Oct 23 12:52:29 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Oct 23 12:52:34 2009 Subject: FW: [Histonet] Floaters in Waterbath Message-ID: <90354A475B420441B2A0396E5008D4967319DD@copc-sbs.COPC.local> One more time. tj -----Original Message----- From: Thomas Jasper Sent: Friday, October 23, 2009 10:41 AM To: 'Stella Mireles' Subject: RE: [Histonet] Floaters in Waterbath Others may mention this to you...and it can get a little political. Do not discount the grossing bench. Whether it's the work of a PA, Pathologist, or other qualified lab staff, the grossing bench should be kept as clean as possible between cases/specimens. I mention the political side because sometimes it gets a bit touchy...histologists may not be in the best position to broach the subject with certain higher level personnel. Especially when the grossing may very well be done under the supervision of said higher level party. >From a patient care standpoint, etc., this definitely should not be an ego-bruiser, as we are all human and make mistakes. But I'm sure most of you know what I'm talking about and probably have experienced something similar at sometime in your careers. One tip I learned from a pathologist, was to keep a clean sponge handy while grossing. This helped a lot, especially with keeping forceps etc., clean in between cases/specimens. Lastly, there are pathologists and PAs out there that keep their egos in check and we are thankful to them. Tom J. Thomas Jasper HT (ASCP) BAS Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 7:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From imilos <@t> cellmarque.com Fri Oct 23 13:01:16 2009 From: imilos <@t> cellmarque.com (Isaac Milos) Date: Fri Oct 23 13:01:22 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: References: Message-ID: <7F2A2AE306CE254DB7279E86A51A7406F3456F@CMROCEX01.cellmarque.local> Hi Naira, Our new IVD rabbit monoclonal (anti human) CD14 is preferentially expressed on monocytes/macrophages: http://www.cellmarque.com/07/p_detail.php?id=2066 Best regards, Isaac Milos Cell Marque Corp. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 8:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Oct 23 13:15:15 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 23 13:15:18 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: <7F2A2AE306CE254DB7279E86A51A7406F3456F@CMROCEX01.cellmarque.local> Message-ID: She is asking to stain for macrophages and that's a monocyte/macrophage marker so that's not the same. You have to be careful with the names of antibodies and check the specification sheets. You can search for a macrophage marker and the antibody may be listed as a macrophage marker as the following example: This antibody is from abcam its listed as follows: Macrophage antibody [MAC387] (ab22506) but when you look at the specification sheet it states -- Recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprising of a 12kD alpha chain and a 14kD beta chain expressed by granulocytes, monocytes and by tissue macrophages. So it is not entirely specific to macrophages. This antibody may work fine for your application, but if you are looking just to stain macrophages this is not the antibody you want to pick. Just a word of advice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Isaac Milos [mailto:imilos@cellmarque.com] Sent: Friday, October 23, 2009 12:01 PM To: Margaryan, Naira; Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Hi Naira, Our new IVD rabbit monoclonal (anti human) CD14 is preferentially expressed on monocytes/macrophages: http://www.cellmarque.com/07/p_detail.php?id=2066 Best regards, Isaac Milos Cell Marque Corp. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 8:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmattison <@t> lakeridgehealth.on.ca Fri Oct 23 13:31:11 2009 From: lmattison <@t> lakeridgehealth.on.ca (Mattison, Lesley) Date: Fri Oct 23 13:31:35 2009 Subject: [Histonet] please unsubscribe Message-ID: <675783845AB33C48BA96A64F82D676B28489D6@LHC-MBX-01.corp.lakeridgehealth.on.ca> Please unsubscribe... thankyou Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From micropathlabs <@t> yahoo.com Fri Oct 23 14:07:57 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Oct 23 14:08:00 2009 Subject: [Histonet] Xylene Substitute Message-ID: <241116.80280.qm@web57808.mail.re3.yahoo.com> Hi all.?Could someone recommend a xylene substitute?and mounting media to use for?staining and coverslipping (no vendors please)? I am considering processing without xylene and would like to discontinue everywhere if?possible. My previous experience was a lifetime ago and I'm sure things have advanced since then. Thank you. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 22 10:34:32 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Oct 23 14:31:09 2009 Subject: [Histonet] Negative controls for special stains (non-organism)?!?! Message-ID: <1AAF670737F193429070841C6B2ADD4CF728279B@EXMBMCB15.ucsfmedicalcenter.org> Our Joint commission audit was just completed (first time for JC for me). We passed almost everything fine. The one thing they came up with is that we don't use "negative controls" for most of the special stains - like trichrome, congo red, etc. We use negative controls for micro-organism cases but not for the others. In fact I've never heard of anyone doing that. Has anyone had this issue with JC? Does anyone run "negative" controls for non-organism special stains? Frankly I'm not sure how that would be done for something like a trichrome. Thanks for any insight! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 22 10:42:45 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Oct 23 14:31:12 2009 Subject: [Histonet] Negative controls for special stains (non-organism)?!?! Message-ID: <1AAF670737F193429070841C6B2ADD4CF72827A7@EXMBMCB15.ucsfmedicalcenter.org> Hi all, Our Joint Commission audit was just completed (first time for JC for me). We passed almost everything fine. The one thing they came up with is that we don't use "negative controls" for most of the special stains - like trichrome, congo red, etc. We use negative controls for micro-organism cases but not for the others. In fact I've never heard of anyone doing that. Has anyone had this issue with JC? Does anyone run "negative" controls for non-organism special stains? Frankly I'm not sure how that would be done for something like a trichrome or other purely tissue-element stains. Thanks for any insight! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA Email: tim.morken@ucsfmedctr.org From Joyce.Cline <@t> wchsys.org Thu Oct 22 11:52:13 2009 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Fri Oct 23 14:31:15 2009 Subject: [Histonet] old microtome kinves Message-ID: How has everyone disposed of their old "permanent" microtome knives? Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 22 14:18:12 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Oct 23 14:31:17 2009 Subject: [Histonet] RE: Plants-in-the-lab OT In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> References: <4D14F0FC9316DD41972D5F03C070908B02E46BB8@nmdamailsvr.nmda.ad.nmsu.edu> <5A2BD13465E061429D6455C8D6B40E390987A86987@IBMB7Exchange.digestivespecialists.com> Message-ID: <1AAF670737F193429070841C6B2ADD4CF73A431B@EXMBMCB15.ucsfmedicalcenter.org> Or it could not handle the hot air in the admin office! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 22, 2009 12:15 PM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants-in-the-lab OT I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 23 12:28:21 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Oct 23 14:31:19 2009 Subject: [Histonet] Test Message-ID: <1AAF670737F193429070841C6B2ADD4CF73A467A@EXMBMCB15.ucsfmedicalcenter.org> Test From carrolpb <@t> umdnj.edu Fri Oct 23 14:42:59 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Oct 23 14:43:07 2009 Subject: [Histonet] please unsubscribe In-Reply-To: <675783845AB33C48BA96A64F82D676B28489D6@LHC-MBX-01.corp.lakeridgehealth.on.ca> References: <675783845AB33C48BA96A64F82D676B28489D6@LHC-MBX-01.corp.lakeridgehealth.on.ca> Message-ID: <4AE20743.4010804@umdnj.edu> > Please unsubscribe... thankyou I'd rather not... I quite like this list! But thanks for the advice...? From rjbuesa <@t> yahoo.com Fri Oct 23 14:55:37 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 23 14:55:42 2009 Subject: [Histonet] Xylene Substitute In-Reply-To: <241116.80280.qm@web57808.mail.re3.yahoo.com> Message-ID: <788238.5624.qm@web65713.mail.ac4.yahoo.com> Sheila: 1- For tissue processing use isopropanol mixed with mineral oil. 2- For dewaxing the sections before staining?use Dish Washing soap. 3- To prepare the slides before coverslipping don't use anything, just oven dry the sections and cover. Under separate cover I am sending the detailed procedures. Ren? J. --- On Fri, 10/23/09, Sheila Haas wrote: From: Sheila Haas Subject: [Histonet] Xylene Substitute To: histonet@lists.utsouthwestern.edu Date: Friday, October 23, 2009, 3:07 PM Hi all.?Could someone recommend a xylene substitute?and mounting media to use for?staining and coverslipping (no vendors please)? I am considering processing without xylene and would like to discontinue everywhere if?possible. My previous experience was a lifetime ago and I'm sure things have advanced since then. Thank you. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From imilos <@t> cellmarque.com Fri Oct 23 14:57:16 2009 From: imilos <@t> cellmarque.com (Isaac Milos) Date: Fri Oct 23 14:57:24 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: References: <7F2A2AE306CE254DB7279E86A51A7406F3456F@CMROCEX01.cellmarque.local> Message-ID: <7F2A2AE306CE254DB7279E86A51A7406F345A0@CMROCEX01.cellmarque.local> Liz, Great point, and definitely something to keep in mind should one do that stain. Indeed, CD14 is not specific to macrophages only (monocytes also express the antigen as discussed). Of course, there are other more specific macrophage markers (i.e. HAM56) but these are commonly developed in mice. The CD14 came to mind as ours is a rabbit antibody. Best regards, Isaac Milos Cell Marque Corporation -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 11:15 AM To: Isaac Milos; Margaryan, Naira; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts She is asking to stain for macrophages and that's a monocyte/macrophage marker so that's not the same. You have to be careful with the names of antibodies and check the specification sheets. You can search for a macrophage marker and the antibody may be listed as a macrophage marker as the following example: This antibody is from abcam its listed as follows: Macrophage antibody [MAC387] (ab22506) but when you look at the specification sheet it states -- Recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprising of a 12kD alpha chain and a 14kD beta chain expressed by granulocytes, monocytes and by tissue macrophages. So it is not entirely specific to macrophages. This antibody may work fine for your application, but if you are looking just to stain macrophages this is not the antibody you want to pick. Just a word of advice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Isaac Milos [mailto:imilos@cellmarque.com] Sent: Friday, October 23, 2009 12:01 PM To: Margaryan, Naira; Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Hi Naira, Our new IVD rabbit monoclonal (anti human) CD14 is preferentially expressed on monocytes/macrophages: http://www.cellmarque.com/07/p_detail.php?id=2066 Best regards, Isaac Milos Cell Marque Corp. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 8:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Oct 23 15:03:49 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Oct 23 15:03:53 2009 Subject: [Histonet] old microtome kinves Message-ID: <57BE698966D5C54EAE8612E8941D7683070B9D28@EXCHANGE3.huntingtonhospital.com> I just threw one out the other day - put it in a sharps container. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, October 22, 2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] old microtome kinves How has everyone disposed of their old "permanent" microtome knives? Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Oct 23 15:15:33 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 23 15:15:38 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: <4AE04432.EF71.003F.0@vet.k-state.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CF5@LSRIEXCH1.lsmaster.lifespan.org> I don't know about plants in the lab (never had any) but I recall a problem from years ago. It was a hot summer day and the air conditioning was having problems, so we opened a couple of screened windows in the lab. At the end of the day one of the techs who was reviewing the slides that had just been stained called me over to look at something she couldn't identify. Neither could I. It looked like a little octopus, a central body with several tapering extensions going off in different directions. The whole object was maybe 100 microns wide. A minute later, another one on a different slide. Then three of them on another section. Some on the H&E slides, some on the special stains. And the objects themselves were stained! Finally figured out that they were something coming from plants outside the windows, not sure what. They didn't look like pollen, but whatever they were they were blowing in the windows, coming right through the screens, settling on the water baths of the techs cutting sections, and getting picked up on the slides with the tissue sections. I did a wipe of the benchtop next to the water baths and there were hundreds of them. So, no more open windows after that. And I would be careful what plants I brought indoors on that basis. From baderbo <@t> gmail.com Fri Oct 23 15:44:22 2009 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Fri Oct 23 15:44:25 2009 Subject: [Histonet] Plants in Histology lab Message-ID: <3c7e700910231344o66c13f12i1d66728c9fc7acec@mail.gmail.com> Everybody is writing about the plants in the histology lab. May be I will share my experience with you in Biochemistry lab at MSU . Many many years ago when I was a post doc in Biochemistry dept. I had lots of plants in the window. One day when I came from lunch, I saw unusual plants along with mine, on close look, they were marijuana plants. I almost had heart attack to see these plants in my cubicle, right away I hid these plants. On investigation we found that our dish washer had these plants some place in the lab. and she was hiding it from the professor. Bader From Maria.Katleba <@t> stjoe.org Fri Oct 23 15:49:00 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Oct 23 15:49:10 2009 Subject: [Histonet] Plants in Histology lab In-Reply-To: <3c7e700910231344o66c13f12i1d66728c9fc7acec@mail.gmail.com> References: <3c7e700910231344o66c13f12i1d66728c9fc7acec@mail.gmail.com> Message-ID: OMG... now that is the best story I have heard all day! Thank you for making me laugh :) Maria -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bader Siddiki Sent: Friday, October 23, 2009 1:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants in Histology lab Everybody is writing about the plants in the histology lab. May be I will share my experience with you in Biochemistry lab at MSU . Many many years ago when I was a post doc in Biochemistry dept. I had lots of plants in the window. One day when I came from lunch, I saw unusual plants along with mine, on close look, they were marijuana plants. I almost had heart attack to see these plants in my cubicle, right away I hid these plants. On investigation we found that our dish washer had these plants some place in the lab. and she was hiding it from the professor. Bader _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From pruegg <@t> ihctech.net Fri Oct 23 15:52:32 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Oct 23 15:53:11 2009 Subject: [Histonet] old microtome kinves In-Reply-To: References: Message-ID: <8963F47F501740CDB62C4E536C14D641@prueggihctechlt> No way, I still use mine, especially the tungsten ones, but I still do some plastic embedding of bone, another place I use mine is in the cryostat to make tape sections of calcified bone. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, October 22, 2009 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] old microtome kinves How has everyone disposed of their old "permanent" microtome knives? Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aj.taylor <@t> blueyonder.co.uk Fri Oct 23 15:59:01 2009 From: aj.taylor <@t> blueyonder.co.uk (alan taylor) Date: Fri Oct 23 15:59:05 2009 Subject: [Histonet] old microtome kinves References: Message-ID: Hi everybody We have had the Sakura disposable blade system for many years. I do recall that we had several rotary and sledge blades that I took to the local scrap metal company, had them weighed and cashed them in, putting the resulting several pounds (?'s)in the Christmas box to go towards the beer on our Christmas night out. Just a suggestion. Regards Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ. UK. Tel: +44 1392 660132 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, October 22, 2009 5:52 PM Subject: [Histonet] old microtome kinves How has everyone disposed of their old "permanent" microtome knives? Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Fri Oct 23 16:27:02 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Oct 23 16:27:09 2009 Subject: [Histonet] Plants in Histology lab In-Reply-To: <3c7e700910231344o66c13f12i1d66728c9fc7acec@mail.gmail.com> References: <3c7e700910231344o66c13f12i1d66728c9fc7acec@mail.gmail.com> Message-ID: <4AE21FA6.5070203@vneubert.com> Dish washer dude :-D That's a really awesome story, almost too much clich?e to be true. Best Friday spam topic anyway. Bader Siddiki schrieb: > Everybody is writing about the plants in the histology lab. > May be I will share my experience with you in Biochemistry lab at MSU . > Many many years ago when I was a post doc in Biochemistry dept. I had lots > of plants in the window. > One day when I came from lunch, I saw unusual plants along with mine, on > close look, they were marijuana plants. > I almost had heart attack to see these plants in my cubicle, right away I > hid these plants. > On investigation we found that our dish washer had these plants some place > in the lab. and she was hiding it from the professor. > Bader > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kim.Osullivan <@t> med.monash.edu.au Fri Oct 23 18:41:55 2009 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Fri Oct 23 18:42:02 2009 Subject: [Histonet] shrinking glomeruli Message-ID: <130.194.114.97.1256341186@my.monash.edu.au> Hi, Does anyone have any suggestions as to why our mouse glomeruli are shrinking after coverlipping in DPX? Immunohistochemistry is performed on PLP frozen cryostat sections, and the glomeruli and tubules look fine upon checking after the chromagen is developed, but once they are dehydrated, cleared in histosol, and then coverslipped in DPX(also tried with Vector hardmount) the glomeruli shrink, and the tubules look like they are spreading out. We have been doing this for years, and not had a problem. Does anyone know why we would have one now? Could the disease state of the tissue contribute? Or earlier fixation- could that contribute downstream? Any suggestions would be appreciated- it is doing my head in! Kim O'Sullivan Department of Medicine Monash Univeristy Australia From tkngflght <@t> yahoo.com Fri Oct 23 22:12:35 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Oct 23 22:12:38 2009 Subject: [Histonet] the not-yet-mentioned benefit of plants in the lab Message-ID: <317367.835.qm@web50907.mail.re2.yahoo.com> I just read of the plant drama for the lab getting a CAP Phase 1 ding.? There was a study done ages and ages ago and certain plants IMPROVE the air quality in chemically contaminated environments.?(If you have any measure of?our solvents in the air-your air is contaminated even if it is an allowable level.)?Live plants also?increase the amount of available oxygen is closed spaces.? ? We used to keep a BUNCH of spider plants--one of the most beneficial species--in our lab for this reason alone.? We also noticed they were quite pretty.? I can only speculate that higher oxygen levels, lower chemical presence and a visually relaxing environment would contribute far more than an occasional bug might detract. ? Just my two cents (sense?), common as they may be. ? Cheryl From estellamireles <@t> gmail.com Fri Oct 23 23:10:12 2009 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Fri Oct 23 23:10:18 2009 Subject: FW: [Histonet] Floaters in Waterbath In-Reply-To: <90354A475B420441B2A0396E5008D4967319DD@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D4967319DD@copc-sbs.COPC.local> Message-ID: Thank You Fellow Histonetters, I greatly appreciate all the advice. Have a great week end. Stella On Fri, Oct 23, 2009 at 12:52 PM, Thomas Jasper wrote: > One more time. > tj > > -----Original Message----- > From: Thomas Jasper > Sent: Friday, October 23, 2009 10:41 AM > To: 'Stella Mireles' > Subject: RE: [Histonet] Floaters in Waterbath > > Others may mention this to you...and it can get a little political. Do > not discount the grossing bench. Whether it's the work of a PA, > Pathologist, or other qualified lab staff, the grossing bench should be > kept as clean as possible between cases/specimens. I mention the > political side because sometimes it gets a bit touchy...histologists may > not be in the best position to broach the subject with certain higher > level personnel. Especially when the grossing may very well be done > under the supervision of said higher level party. > > >From a patient care standpoint, etc., this definitely should not be an > ego-bruiser, as we are all human and make mistakes. But I'm sure most > of you know what I'm talking about and probably have experienced > something similar at sometime in your careers. > > One tip I learned from a pathologist, was to keep a clean sponge handy > while grossing. This helped a lot, especially with keeping forceps > etc., clean in between cases/specimens. Lastly, there are pathologists > and PAs out there that keep their egos in check and we are thankful to > them. > > Tom J. > > Thomas Jasper HT (ASCP) BAS > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella > Mireles > Sent: Friday, October 23, 2009 7:11 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Floaters in Waterbath > > I know we have all had some problems with floaters in our waterbath at > some point in our microtomy career. > Our doctors are very picky and I need some tips on keeping an immaculate > clean waterbath, but not sacrificing the speed in a regular routine lab. > We use the pyrex waterbath and paper towels for wiping our area. > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mikoff <@t> pacbell.net Sat Oct 24 01:51:41 2009 From: mikoff <@t> pacbell.net (Keith) Date: Sat Oct 24 01:50:38 2009 Subject: [Histonet] Vol 71, Issue 28, 1. Re: Collagen staining using special stains, In-Reply-To: <200910231837.n9NIb9q1009256@flpd125.prodigy.net> Message-ID: Collagen cannot be expected to stain uniformly throughout, since "collagen" is a blanket term for at least 10 different chemical substances; therefore, certain areas of the collagen will take on the dye with different intensity. I like the comments made by Geoff about fixation and removing the paraffin, which I'm sure you've already addressed. His comment about not drawing any conclusions about the collage through the use of special stains is important. If the investigator is not satisfied with the standard collagen stains using analine blue, fast green, or acid fuchsin, then they might want to try some IHC or tapping-mode atomic force microscopy to further identify the subtypes of the collegen. Keith M. Mikoff HTL/HT(ASCP) From gu.lang <@t> gmx.at Sat Oct 24 03:45:05 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Oct 24 03:45:14 2009 Subject: AW: [Histonet] Masson's tri-chrome specificity In-Reply-To: <5F7CC9B788911848A79BC83453A3D6530360CF3CC8@Tomlinson.hti.com> References: <5F7CC9B788911848A79BC83453A3D6530360CF3CC8@Tomlinson.hti.com> Message-ID: <4B41C9B67C7F49D680BF06EF21C4226A@dielangs.at> Jennifer, I don't think, that you can substitute Biebrich Scarlett with Eosin. These dyes have a different chemistry. That is well described on Bryan Llewellyn's website: http://stainsfile.info/StainsFile/stain/trichindex.htm The staining of collagen is also a matter of differentiation of the dyes in water and diluted ethanol. Biebrich Scarlett "leaves" the tissue faster than anilinblue in diluted ethanol. So if you want blue collagen, wash longer in 96%. This will also lead to paler cytoplasm staining. There is a publication on the different dye uptake of collagen, that was exposed to the atmosphere (Histologic 1979). Older blocks show uneven dye uptake. And another publication refers to the tension-state of collagen: "The Masson staining of collagen - an explanation of an apparent paradox" The Histochemical Journal 1975. It says that in tensioned collagen more positivly-charged amino dye-binding sites are available than in relaxed collagen. That leads to better dye uptake of arylmethan dyes of collagen in streched tendon or stretched dermis. I think ripened collagen with many crosslinks has also less dye-binding sites than "younger" collagen. Are there more recent publication on this issue? Gudrun Lang Histolab, Akh Linz -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Anderson Gesendet: Freitag, 23. Oktober 2009 19:52 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Masson's tri-chrome specificity Good morning Histonet. Thanks a lot to so many people who helped me with my Leica XL question - your wealth of knowledge never ceases to impress me. I have a different question now about Masson's tri-chrome and it's specificity on isolated rabbit tendon. There is some muscle still attached to the ends of the tendon. We get both Beibrich Scarlet and Aniline Blue staining, at different intensities, within each collagen sample (even control). How can I explain this? Would there be a possibility to omit the Beibrich and stain with eosin and Aniline blue? Thanks so much! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Oct 24 12:18:12 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Oct 24 12:18:15 2009 Subject: [Histonet] Cleanliness at the Grossing Bench(was Floaters in Waterbath) Message-ID: It's really important to clean up as you go, when you gross tissues. An overworked Pathologist's Assistant (PA) - who never sees the slides - can carry over an awful lot of tissue and not realize the problem that creates. By ancient custom one may only have one of each kind of tool - scalpel, scissors, tweezers, ruler - at a grossing desk. If I ran the zoo I'd have about five of each, and toss them into a pot of water after each case, pausing to clean the tools after every five cases. Rinsing one's gloved hands is important. I wouldn't want a sponge - too hard to clean - but paper towels are cheap. I can gross much faster with the help of an assistant, but this help has become less and less common in recent years. Unlike a dentist or a psychiatrist, a pathologist's time is worth nothing. Who cleans up at the end of the day? Usually whoever assists, but lately I've had a job where I had to scrub the place after the placentas were done. Why didn't I go into radiology? Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Sat Oct 24 12:22:04 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Oct 24 12:22:08 2009 Subject: [Histonet] Re: Xylene Substitute Message-ID: Sheila Hass at Micro Path Laboratories [where?} asks: >>Could someone recommend a xylene substitute?and mounting media to use for?staining and coverslipping (no vendors please)? I am considering processing without xylene, and would like to discontinue it everywhere if possible.<< We've discussed this topic a number of times before - see the archives for quite a lot of information. Basically there are two classes of alternative solvents, limonene and aliphatic hydrocarbons. Aliphatics go by brand name, are not equivalent, and should not be mixed. Aliphatics, like xylene, can be about 85% recovered by distillation. As far as I know, all resin mounting media contain aromatics (xylene, toluene, or benzene) - no practical work-around for that - so you'll still need a hood for coverslipping. Bob Richmond Samurai Pathologist Knoxville TN From pruegg <@t> ihctech.net Sat Oct 24 13:07:53 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Oct 24 13:08:35 2009 Subject: SPAM-LOW: [Histonet] RE: Granzyme b and F4/80 In-Reply-To: References: Message-ID: <09E4C22CE3154F3E97655C2D69084260@ihctechq9h2qof> Jennifer, Your NS background could be coming from endogenous biotin. I use the rat anti ms F4/80 on ffpe mouse tissue all the time and it is very clean but I use a labeled polymer detection system rather than AB. I use rat on mouse hrp or ap labeled polymer detection from BioCare, or I use my own link secondary that is rab anti rat and then follow with hrp rabbit labeled polymer (PolyVision from Leica). I do use a protein block before the primary but no serum block. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PALMER Jason (SVHM) Sent: Wednesday, October 21, 2009 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: Granzyme b and F4/80 For Serotec F4/80, we use 10% rabbit serum as block and 5% of the same as antibody diluent -our secondary is a biotinylated rabbit anti rat. We also retrieve with Dako proteinase K rather than citrate, with simple HRP-streptavidin then DAB, and it all works very nicely, no background issues. Hope this helps, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Hi All, I'm currently trying to work up Granzyme B and F4/80 on FFPE mouse tissue. I seemed to get a bit of non-specific staining for both and was wondering if there is any way I can eliminate some of this. For Granzyme B I am using a rabbit polyclonal from Abcam. My protocol goes like this: H202 block, citrate buffer HIER, Powerblock (casein-based, non-serum protein block), primary antibody, secondary antibody (rabbit-on rodent HRP polymer, from biocare), DAB, counterstain. My F4/80 is a rat anti-mouse primary from Serotec and the protocol is as follows: H202 block, citrate buffer HIER, Powerblock, avidin/biotin blocks, primary antibody, secondary antibody (Goat anti-rat, biotinylated, mouse-absorbed, from Biocare), HRP Label, DAB, counterstain. Is it true that if I switched to a serum block from the species in which the secondary was raised, instead of my non-serum protein block, it may help? Thank you in advance! Jennifer Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Sat Oct 24 16:56:18 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Oct 24 16:56:25 2009 Subject: [Histonet] Floors Message-ID: <90354A475B420441B2A0396E5008D4967319E4@copc-sbs.COPC.local> My thanks to everyone for their input about cleaning lab floors. Much appreciated. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net From lpwenk <@t> sbcglobal.net Sat Oct 24 23:11:03 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Oct 24 23:11:14 2009 Subject: [Histonet] Masson's tri-chrome specificity In-Reply-To: <4B41C9B67C7F49D680BF06EF21C4226A@dielangs.at> Message-ID: Try extending the time in Bouins. Tendon is rather dense, and may need longer time in the post-mordant. 60 degrees C for 1 hour, with a good 5-10 minute wash in running water afterwards, to remove all the yellow. The same with the phosphotungstic acid rinse between the red and blue dyes. Make it 5 minutes. Other substitutes for Biebrich scarlet are Ponceau de xylidene, Ponceau S, Acid fuchsin, Chromotrope 2R. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, October 24, 2009 4:45 AM To: 'Jennifer Anderson' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Masson's tri-chrome specificity Jennifer, I don't think, that you can substitute Biebrich Scarlett with Eosin. These dyes have a different chemistry. That is well described on Bryan Llewellyn's website: http://stainsfile.info/StainsFile/stain/trichindex.htm The staining of collagen is also a matter of differentiation of the dyes in water and diluted ethanol. Biebrich Scarlett "leaves" the tissue faster than anilinblue in diluted ethanol. So if you want blue collagen, wash longer in 96%. This will also lead to paler cytoplasm staining. There is a publication on the different dye uptake of collagen, that was exposed to the atmosphere (Histologic 1979). Older blocks show uneven dye uptake. And another publication refers to the tension-state of collagen: "The Masson staining of collagen - an explanation of an apparent paradox" The Histochemical Journal 1975. It says that in tensioned collagen more positivly-charged amino dye-binding sites are available than in relaxed collagen. That leads to better dye uptake of arylmethan dyes of collagen in streched tendon or stretched dermis. I think ripened collagen with many crosslinks has also less dye-binding sites than "younger" collagen. Are there more recent publication on this issue? Gudrun Lang Histolab, Akh Linz -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Anderson Gesendet: Freitag, 23. Oktober 2009 19:52 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Masson's tri-chrome specificity Good morning Histonet. Thanks a lot to so many people who helped me with my Leica XL question - your wealth of knowledge never ceases to impress me. I have a different question now about Masson's tri-chrome and it's specificity on isolated rabbit tendon. There is some muscle still attached to the ends of the tendon. We get both Beibrich Scarlet and Aniline Blue staining, at different intensities, within each collagen sample (even control). How can I explain this? Would there be a possibility to omit the Beibrich and stain with eosin and Aniline blue? Thanks so much! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Sun Oct 25 13:34:19 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Oct 25 13:34:42 2009 Subject: [Histonet] Texas Society for Histotechnology 2010 Meeting Message-ID: <8CC23B8E64EE12A-32DC-1AE4F@webmail-m051.sysops.aol.com> All: The Texas Society for Histotechology Program Committee is in the process of accepting abstracts for the upcoming 2010 State meeting in Houston,Texas April 23-25, 2010. If you or someone you know would be interested in presenting a symposium and/or workshop please contact me at kdwyer3322@aol.com for more information. Regards, Kathy Dwyer TSH Convention Coordinator From pruegg <@t> ihctech.net Sun Oct 25 16:59:21 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 25 17:01:08 2009 Subject: [Histonet] human macrophages in human melanoma xenografts In-Reply-To: <7F2A2AE306CE254DB7279E86A51A7406F345A0@CMROCEX01.cellmarque.local> References: <7F2A2AE306CE254DB7279E86A51A7406F3456F@CMROCEX01.cellmarque.local> <7F2A2AE306CE254DB7279E86A51A7406F345A0@CMROCEX01.cellmarque.local> Message-ID: <0259366A28EB40D5A4527EF54C104904@prueggihctechlt> I do not know of a non mouse anti human macrophage marker, but in my experience most are using the wrong ms monoclonal anti human CD68 for macrophage markers, The KP1 clone will stain a lot more than macrophages, granulocytes etc., the best clone to use for anti human CD68 is the PGM1 clone which is specific to just macrophages and monocytes and not all the other cells. I have known hematopathologist who tell me they stopped using CD68 because it was just not specific enough, but when I tell them to switch to the PGM1 clone they are very happy with that. As for macrophages in mouse tissue, I use the serotec rat anti mouse F4/80 ab with very good results. I have used macrophage markers that are for rat tissue (can't remember what they are at the moment, I think the are CD68?) but I did not have as good results on rat tissue with them as I did on ms tissue with rat anti f4/80. Liz, What do you use for macs on rat tissue? I may have asked you about this before? Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Isaac Milos Sent: Friday, October 23, 2009 1:57 PM To: Liz Chlipala; Margaryan, Naira; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Liz, Great point, and definitely something to keep in mind should one do that stain. Indeed, CD14 is not specific to macrophages only (monocytes also express the antigen as discussed). Of course, there are other more specific macrophage markers (i.e. HAM56) but these are commonly developed in mice. The CD14 came to mind as ours is a rabbit antibody. Best regards, Isaac Milos Cell Marque Corporation -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 11:15 AM To: Isaac Milos; Margaryan, Naira; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts She is asking to stain for macrophages and that's a monocyte/macrophage marker so that's not the same. You have to be careful with the names of antibodies and check the specification sheets. You can search for a macrophage marker and the antibody may be listed as a macrophage marker as the following example: This antibody is from abcam its listed as follows: Macrophage antibody [MAC387] (ab22506) but when you look at the specification sheet it states -- Recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprising of a 12kD alpha chain and a 14kD beta chain expressed by granulocytes, monocytes and by tissue macrophages. So it is not entirely specific to macrophages. This antibody may work fine for your application, but if you are looking just to stain macrophages this is not the antibody you want to pick. Just a word of advice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Isaac Milos [mailto:imilos@cellmarque.com] Sent: Friday, October 23, 2009 12:01 PM To: Margaryan, Naira; Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Hi Naira, Our new IVD rabbit monoclonal (anti human) CD14 is preferentially expressed on monocytes/macrophages: http://www.cellmarque.com/07/p_detail.php?id=2066 Best regards, Isaac Milos Cell Marque Corp. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 8:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:liz@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Mon Oct 26 07:04:48 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Oct 26 07:04:52 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: <4AE18838.2B7F.00C9.0@geisinger.edu> References: <4AE18838.2B7F.00C9.0@geisinger.edu> Message-ID: <982550.7430.qm@web50303.mail.re2.yahoo.com> I have always used?pieces of phone book paper.? Just ask everyone?to bring in their old phone books,?and tear the?papers off at the seam.??They provide just the right amount of absorption, are the perfect size, are free and a useful way to recycle! Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Angela Bitting To: Jackie M O'Connor ; Stella Mireles ; histonet-bounces@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Sent: Fri, October 23, 2009 10:40:58 AM Subject: Re: [Histonet] Floaters in Waterbath We currently have a Quality Improvement Plan in effect to address this issue. Jackie is right about keeping those forcep wells clean. Although we don't swipe Kimwipes over our waterbath after each block, we do it very regularly. Another thing to consider is how often you clean your embedding molds. ~Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 ! >>> "Jackie M O'Connor" 10/23/2009 10:23 AM >>> Kim wipes seem to pick up more debris than paper towels, and they pick up much less water.? We routinely sweep the waterbath with a kimwipe after each block.? You can also pick up floaters from embedding if the forceps are not cleaned between each block.? Most embedding centers have multiple wells for forceps - how often do you clean those wells?? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-bounces@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab.? We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From ree3 <@t> leicester.ac.uk Mon Oct 26 07:23:46 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Oct 26 07:23:51 2009 Subject: [Histonet] RE: Plants in the Histology Lab In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CF5@LSRIEXCH1.lsmaster.lifespan.org> References: <4AE04432.EF71.003F.0@vet.k-state.edu> <4EBFF65383B74D49995298C4976D1D5E03835CF5@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C6305300@EXC-MBX3.cfs.le.ac.uk> They are a type of fungal spore, probably Tetraploa spp.,,,,,,,,,,,,,, -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: 23 October 2009 21:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants in the Histology Lab I don't know about plants in the lab (never had any) but I recall a problem from years ago. It was a hot summer day and the air conditioning was having problems, so we opened a couple of screened windows in the lab. At the end of the day one of the techs who was reviewing the slides that had just been stained called me over to look at something she couldn't identify. Neither could I. It looked like a little octopus, a central body with several tapering extensions going off in different directions. The whole object was maybe 100 microns wide. A minute later, another one on a different slide. Then three of them on another section. Some on the H&E slides, some on the special stains. And the objects themselves were stained! Finally figured out that they were something coming from plants outside the windows, not sure what. They didn't look like pollen, but whatever they were they were blowing in the windows, coming right through the screens, settling on the water baths of the techs cutting sections, and getting picked up on the slides with the tissue sections. I did a wipe of the benchtop next to the water baths and there were hundreds of them. So, no more open windows after that. And I would be careful what plants I brought indoors on that basis. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly_colpitts <@t> hotmail.com Mon Oct 26 07:39:32 2009 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Mon Oct 26 07:39:37 2009 Subject: [Histonet] CMV control slide Message-ID: Good Morning Histoland, Just wondering if anyone knows where I can purchase CMV control slides. Normally we buy ours from Newcomer but according to their website, they are currently out of stock. Thanks for your help, Kelly Colpitts, HT (ASCP) _________________________________________________________________ Windows 7: It works the way you want. Learn more. http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen2:102009 From cmiller <@t> physlab.com Mon Oct 26 08:01:06 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Oct 26 08:01:11 2009 Subject: [Histonet] (no subject) Message-ID: test Cheryl Miller HT ASCP Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Jackie.O'Connor <@t> abbott.com Mon Oct 26 08:09:01 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Oct 26 08:09:27 2009 Subject: [Histonet] Open Position In-Reply-To: <8CC23B8E64EE12A-32DC-1AE4F@webmail-m051.sysops.aol.com> References: <8CC23B8E64EE12A-32DC-1AE4F@webmail-m051.sysops.aol.com> Message-ID: I have an open position for a Histotech in a GLP research lab in the Chicago area. Direct inquiries to me at the email address below. Jackie.O'Connor@abbott.com From jerry.santiago <@t> jax.ufl.edu Mon Oct 26 08:10:07 2009 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Mon Oct 26 08:10:15 2009 Subject: [Histonet] Florida Society for Histotechnology Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF30148F52E@jaxmail.umc.ufl.edu> The Florida Society for Histotechnology is accepting abstracts for their 2010 meeting. FSH will be the hosting state for the NSH Region III Meeting. The meeting will be held at the Double Tree International in Orlando, Florida from April 22 - 25, 2010. Abstracts form can be downloaded from our website at www.fshgroup.org and should be submitted by December 1, 2009. All abstracts may be faxed to 904-621-9125 or e-mailed to sclark@fshgroup.org. For further information please feel free to contact Susan Clark, FSH President at 954-265-5371 or by e-mail at sclark@fshgroup.org. Sincerely, Susan Clark, President Florida Society for Histotechnology From Dorothy.L.Webb <@t> HealthPartners.Com Mon Oct 26 09:39:41 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Oct 26 09:39:45 2009 Subject: [Histonet] Negative control Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43A0804BDEC5@HPEMX3.HealthPartners.int> We have not done negative controls, but, have recently started placing both a negative and positive control on slides for Congo Red based on recommendation by Mayo. We are using a keloid scar. They state that on consult cases, they have often noticed overstaining on high collagen cases. Our pathologists thought it would be good for comparison! But, that is the only one for now!! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From brett_connolly <@t> merck.com Mon Oct 26 09:52:58 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Oct 26 09:53:10 2009 Subject: [Histonet] Floaters in Waterbath In-Reply-To: <982550.7430.qm@web50303.mail.re2.yahoo.com> References: <4AE18838.2B7F.00C9.0@geisinger.edu> <982550.7430.qm@web50303.mail.re2.yahoo.com> Message-ID: <63EA0607835FBA4689CEA9EA8B482692026E5ACE@usctmx1141.merck.com> Agree that phone book paper works great on the water. Picked up that tip many years ago from the AFIP. Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, October 26, 2009 8:05 AM To: Angela Bitting; Jackie M O'Connor; Stella Mireles; histonet-bounces@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Floaters in Waterbath I have always used?pieces of phone book paper.? Just ask everyone?to bring in their old phone books,?and tear the?papers off at the seam.??They provide just the right amount of absorption, are the perfect size, are free and a useful way to recycle! Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Angela Bitting To: Jackie M O'Connor ; Stella Mireles ; histonet-bounces@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Sent: Fri, October 23, 2009 10:40:58 AM Subject: Re: [Histonet] Floaters in Waterbath We currently have a Quality Improvement Plan in effect to address this issue. Jackie is right about keeping those forcep wells clean. Although we don't swipe Kimwipes over our waterbath after each block, we do it very regularly. Another thing to consider is how often you clean your embedding molds. ~Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 ! >>> "Jackie M O'Connor" 10/23/2009 10:23 AM >>> Kim wipes seem to pick up more debris than paper towels, and they pick up much less water.? We routinely sweep the waterbath with a kimwipe after each block.? You can also pick up floaters from embedding if the forceps are not cleaned between each block.? Most embedding centers have multiple wells for forceps - how often do you clean those wells?? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-bounces@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab.? We use the pyrex waterbath and paper towels for wiping our area. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From akemiat3377 <@t> yahoo.com Mon Oct 26 10:14:25 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Oct 26 10:14:31 2009 Subject: [Histonet] NSH Website down again Message-ID: <52920.49035.qm@web113802.mail.gq1.yahoo.com> Hi out there, The NSH website is down again. ?I tried this weekend, as well as this morning and this is what comes-up. ?Warning: file(http://www.e-guana.net/organizations.php3?action=printContentItem&orgid=111&typeID=1234&itemID=19243) [function.file]: failed to open stream: HTTP request failed! HTTP/1.1 404 Not Found in?/home/nsh/nsh-www/organizations.php3?on line?23 Warning: implode() [function.implode]: Bad arguments. in?/home/nsh/nsh-www/organizations.php3?on line?23 Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com From cgill <@t> marylandgeneral.org Mon Oct 26 10:30:55 2009 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Mon Oct 26 10:31:02 2009 Subject: [Histonet] Steiner stain on nexes Message-ID: <7E37482715185C4494F24AC3F17BE072018CCAFF@mdgen-exch.marylandgeneral.org> Ventana will be sending a Technical Rep. out on Thur. to our lab I'll keep it posted as to whether or not there is a resolution with our steiner stain. __________________________________________________________________________________________________________________________ This message, together with any attachments, is confidential and intended only for the use of the individual or entity to which it is addressed. This information may be privileged and otherwise protected by state and federal law. If you are not the intended recipient,any disclosure, copying, distribution or any other use of the contents of this message is prohibited. If you have received this message in error, please notify the sender immediately by telephone or by return e-mail and delete this message along with any attachments. __________________________________________________________________________________________________________________________ From pruegg <@t> ihctech.net Mon Oct 26 10:41:03 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 26 10:41:39 2009 Subject: [Histonet] RE: Antigen retrieval question In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF72820A6@EXMBMCB15.ucsfmedicalcenter.org> References: <63EA0607835FBA4689CEA9EA8B482692026E4C5B@usctmx1141.merck.com> <1AAF670737F193429070841C6B2ADD4CF72820A6@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <0B578E84AF29423B99039ACDE53BBE0B@Patsyoffice> Yes, I have, redoing the AR does fix that though. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, October 20, 2009 12:29 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Antigen retrieval question Brett, how long? I've left them overnight before proceeding without any problems. It would not be "re-fixation" as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, October 20, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval question Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Oct 26 10:48:42 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Oct 26 10:48:50 2009 Subject: [Histonet] the not-yet-mentioned benefit of plants in the lab In-Reply-To: <317367.835.qm@web50907.mail.re2.yahoo.com> References: <317367.835.qm@web50907.mail.re2.yahoo.com> Message-ID: So there seem to be 2 trains of thought Histoland regarding plants in the lab: 1. Plants are GOOD for both physical and psychological health 2. Plants are BAD because they spread fungus and bacteria and allergens. Soooo....why not include plants that are the least allergenic while taking measures to limit the contamination they (may) cause? Just a thought...I love my spider plants and philodendrons... Regards, Merced --On Friday, October 23, 2009 8:12 PM -0700 Cheryl wrote: > I just read of the plant drama for the lab getting a CAP Phase 1 ding.? > There was a study done ages and ages ago and certain plants IMPROVE the > air quality in chemically contaminated environments.?(If you have any > measure of?our solvents in the air-your air is contaminated even if it > is an allowable level.)?Live plants also?increase the amount of > available oxygen is closed spaces.? ? > We used to keep a BUNCH of spider plants--one of the most beneficial > species--in our lab for this reason alone.? We also noticed they were > quite pretty.? I can only speculate that higher oxygen levels, lower > chemical presence and a visually relaxing environment would contribute > far more than an occasional bug might detract. ? > Just my two cents (sense?), common as they may be. > ? > Cheryl > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From ree3 <@t> leicester.ac.uk Mon Oct 26 10:59:33 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Oct 26 10:59:41 2009 Subject: [Histonet] the not-yet-mentioned benefit of plants in the lab In-Reply-To: References: <317367.835.qm@web50907.mail.re2.yahoo.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C6305310@EXC-MBX3.cfs.le.ac.uk> Or how about non-allergenic silk flowers or plastic bonsai trees, they always look the real thing after a glass of red or two............... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: 26 October 2009 15:49 To: Cheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] the not-yet-mentioned benefit of plants in the lab So there seem to be 2 trains of thought Histoland regarding plants in the lab: 1. Plants are GOOD for both physical and psychological health 2. Plants are BAD because they spread fungus and bacteria and allergens. Soooo....why not include plants that are the least allergenic while taking measures to limit the contamination they (may) cause? Just a thought...I love my spider plants and philodendrons... Regards, Merced --On Friday, October 23, 2009 8:12 PM -0700 Cheryl wrote: > I just read of the plant drama for the lab getting a CAP Phase 1 ding.? > There was a study done ages and ages ago and certain plants IMPROVE the > air quality in chemically contaminated environments.?(If you have any > measure of?our solvents in the air-your air is contaminated even if it > is an allowable level.)?Live plants also?increase the amount of > available oxygen is closed spaces.? ? > We used to keep a BUNCH of spider plants--one of the most beneficial > species--in our lab for this reason alone.? We also noticed they were > quite pretty.? I can only speculate that higher oxygen levels, lower > chemical presence and a visually relaxing environment would contribute > far more than an occasional bug might detract. ? > Just my two cents (sense?), common as they may be. > ? > Cheryl > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Oct 26 11:07:28 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Oct 26 11:07:33 2009 Subject: [Histonet] the not-yet-mentioned benefit of plants in the lab In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8C6305310@EXC-MBX3.cfs.le.ac.uk> References: <317367.835.qm@web50907.mail.re2.yahoo.com> <7722595275A4DD4FA225B92CDBF174A1E8C6305310@EXC-MBX3.cfs.le.ac.uk> Message-ID: <016D874E03F3B61E03A71E16@CDYwxp1931.ad.med.buffalo.edu> Hahaha...if you can create them with filters for sucking out the formaldehyde and xylene fumes...! --On Monday, October 26, 2009 3:59 PM +0000 "Edwards, R.E." wrote: > Or how about non-allergenic silk flowers or plastic bonsai trees, they > always look the real thing after a glass of red or two............... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M > Leiker Sent: 26 October 2009 15:49 > To: Cheryl; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] the not-yet-mentioned benefit of plants in the lab > > So there seem to be 2 trains of thought Histoland regarding plants in the > lab: > > 1. Plants are GOOD for both physical and psychological health > 2. Plants are BAD because they spread fungus and bacteria and allergens. > > Soooo....why not include plants that are the least allergenic while > taking measures to limit the contamination they (may) cause? > > Just a thought...I love my spider plants and philodendrons... > > Regards, > Merced > > --On Friday, October 23, 2009 8:12 PM -0700 Cheryl > wrote: > >> I just read of the plant drama for the lab getting a CAP Phase 1 ding.? >> There was a study done ages and ages ago and certain plants IMPROVE the >> air quality in chemically contaminated environments.?(If you have any >> measure of?our solvents in the air-your air is contaminated even if it >> is an allowable level.)?Live plants also?increase the amount of >> available oxygen is closed spaces.? ? >> We used to keep a BUNCH of spider plants--one of the most beneficial >> species--in our lab for this reason alone.? We also noticed they were >> quite pretty.? I can only speculate that higher oxygen levels, lower >> chemical presence and a visually relaxing environment would contribute >> far more than an occasional bug might detract. ? >> Just my two cents (sense?), common as they may be. >> ? >> Cheryl >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From KMB01 <@t> grh.org Mon Oct 26 11:10:21 2009 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Mon Oct 26 11:10:26 2009 Subject: [Histonet] New cassette printer and Slide Writer In-Reply-To: Message-ID: I Would like this information also please. We are a small lab but would like to look into something like this. Thanks, Kathy Gorham, H.T. Grande Ronde Hospital GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Sharon Sent: Thursday, October 22, 2009 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New cassette printer and Slide Writer Hello Histo-world, I am beginning the search for a new cassette printer and slide printer. I would appreciate any feedback on these items. Thank you. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line scampbell@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Oct 26 11:11:57 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 26 11:12:06 2009 Subject: [Histonet] the not-yet-mentioned benefit of plants in the lab In-Reply-To: <016D874E03F3B61E03A71E16@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <478739.42114.qm@web65707.mail.ac4.yahoo.com> Isn't out there some "purist" that could consider that using plants to purify the air from noxious fumes could be a case of "plant cruelty"???!!! (Like the "canary in the mine"?) Ren? J. --- On Mon, 10/26/09, Merced M Leiker wrote: From: Merced M Leiker Subject: RE: [Histonet] the not-yet-mentioned benefit of plants in the lab To: "Edwards, R.E." , "Cheryl" , histonet@lists.utsouthwestern.edu Date: Monday, October 26, 2009, 12:07 PM Hahaha...if you can create them with filters for sucking out the formaldehyde and xylene fumes...! --On Monday, October 26, 2009 3:59 PM +0000 "Edwards, R.E." wrote: > Or how about non-allergenic? silk flowers or? plastic bonsai trees, they > always look the? real thing after a???glass? of red or two............... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M > Leiker Sent: 26 October 2009 15:49 > To: Cheryl; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] the not-yet-mentioned benefit of plants in the lab > > So there seem to be 2 trains of thought Histoland regarding plants in the > lab: > > 1. Plants are GOOD for both physical and psychological health > 2. Plants are BAD because they spread fungus and bacteria and allergens. > > Soooo....why not include plants that are the least allergenic while > taking? measures to limit the contamination they (may) cause? > > Just a thought...I love my spider plants and philodendrons... > > Regards, > Merced > > --On Friday, October 23, 2009 8:12 PM -0700 Cheryl > wrote: > >> I just read of the plant drama for the lab getting a CAP Phase 1 ding.? >> There was a study done ages and ages ago and certain plants IMPROVE the >> air quality in chemically contaminated environments.?(If you have any >> measure of?our solvents in the air-your air is contaminated even if it >> is an allowable level.)?Live plants also?increase the amount of >> available oxygen is closed spaces.?? ? >> We used to keep a BUNCH of spider plants--one of the most beneficial >> species--in our lab for this reason alone.? We also noticed they were >> quite pretty.? I can only speculate that higher oxygen levels, lower >> chemical presence and a visually relaxing environment would contribute >> far more than an occasional bug might detract. ? >> Just my two cents (sense?), common as they may be. >> ? >> Cheryl >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214? USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Oct 26 11:31:29 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Oct 26 11:29:39 2009 Subject: [Histonet] the not-yet-mentioned benefit of plants in the lab In-Reply-To: <478739.42114.qm@web65707.mail.ac4.yahoo.com> References: <478739.42114.qm@web65707.mail.ac4.yahoo.com> Message-ID: <4AE5CEE1.1040006@umdnj.edu> Plants have feelings, too. Geoff Rene J Buesa wrote: > Isn't out there some "purist" that could consider that using plants to purify the air from noxious fumes could be a case of "plant cruelty"???!!! (Like the "canary in the mine"?) > Ren? J. > > --- On Mon, 10/26/09, Merced M Leiker wrote: > > > From: Merced M Leiker > Subject: RE: [Histonet] the not-yet-mentioned benefit of plants in the lab > To: "Edwards, R.E." , "Cheryl" , histonet@lists.utsouthwestern.edu > Date: Monday, October 26, 2009, 12:07 PM > > > Hahaha...if you can create them with filters for sucking out the > formaldehyde and xylene fumes...! > > --On Monday, October 26, 2009 3:59 PM +0000 "Edwards, R.E." > wrote: > > >> Or how about non-allergenic silk flowers or plastic bonsai trees, they >> always look the real thing after a glass of red or two............... >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M >> Leiker Sent: 26 October 2009 15:49 >> To: Cheryl; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] the not-yet-mentioned benefit of plants in the lab >> >> So there seem to be 2 trains of thought Histoland regarding plants in the >> lab: >> >> 1. Plants are GOOD for both physical and psychological health >> 2. Plants are BAD because they spread fungus and bacteria and allergens. >> >> Soooo....why not include plants that are the least allergenic while >> taking measures to limit the contamination they (may) cause? >> >> Just a thought...I love my spider plants and philodendrons... >> >> Regards, >> Merced >> >> --On Friday, October 23, 2009 8:12 PM -0700 Cheryl >> wrote: >> >> >>> I just read of the plant drama for the lab getting a CAP Phase 1 ding. >>> There was a study done ages and ages ago and certain plants IMPROVE the >>> air quality in chemically contaminated environments. (If you have any >>> measure of our solvents in the air-your air is contaminated even if it >>> is an allowable level.) Live plants also increase the amount of >>> available oxygen is closed spaces. >>> We used to keep a BUNCH of spider plants--one of the most beneficial >>> species--in our lab for this reason alone. We also noticed they were >>> quite pretty. I can only speculate that higher oxygen levels, lower >>> chemical presence and a visually relaxing environment would contribute >>> far more than an occasional bug might detract. >>> Just my two cents (sense?), common as they may be. >>> >>> Cheryl >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> Merced M Leiker >> Research Technician II >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From SDrew <@t> uwhealth.org Mon Oct 26 11:46:16 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Mon Oct 26 11:46:26 2009 Subject: [Histonet] Need new ALK vendor Message-ID: <738A7878143FF74BB77436E255743C1A1F7CE2@UWHC-MAIL03.uwhis.hosp.wisc.edu> We are looking for a new anti-ALK antibody for formalin-fixed, paraffin-embedded tissue. If anyone would like to share their favorite source I'd certainly appreciate it. Hate to just throw a dart in the catalog cupboard, or spend lots of time getting information online without a nudge in any certain direction! Thank you! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From lblazek <@t> digestivespecialists.com Mon Oct 26 11:51:19 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Oct 26 11:46:36 2009 Subject: [Histonet] NSH Website down again In-Reply-To: <52920.49035.qm@web113802.mail.gq1.yahoo.com> References: <52920.49035.qm@web113802.mail.gq1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A869B2@IBMB7Exchange.digestivespecialists.com> Oh no! They are having bad arguments and are going to implode! It can't be! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Monday, October 26, 2009 11:14 AM To: histonet; NSH Subject: [Histonet] NSH Website down again Hi out there, The NSH website is down again. ?I tried this weekend, as well as this morning and this is what comes-up. ?Warning: file(http://www.e-guana.net/organizations.php3?action=printContentItem&orgid=111&typeID=1234&itemID=19243) [function.file]: failed to open stream: HTTP request failed! HTTP/1.1 404 Not Found in?/home/nsh/nsh-www/organizations.php3?on line?23 Warning: implode() [function.implode]: Bad arguments. in?/home/nsh/nsh-www/organizations.php3?on line?23 Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Mon Oct 26 11:55:16 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Oct 26 11:55:21 2009 Subject: [Histonet] Need new ALK vendor In-Reply-To: <738A7878143FF74BB77436E255743C1A1F7CE2@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645CD7@chi2k3ms01.columbuschildrens.net> Anaplastic Lymphoma Kinase, clone 5A4, Leica Microsystems. They have concentrated, NCL-ALK, and a predilute for the BondMax, PA0306. Excellent crisp staining with EDTA retrieval Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Sally A Sent: Monday, October 26, 2009 12:46 PM To: Histonet Subject: [Histonet] Need new ALK vendor We are looking for a new anti-ALK antibody for formalin-fixed, paraffin-embedded tissue. If anyone would like to share their favorite source I'd certainly appreciate it. Hate to just throw a dart in the catalog cupboard, or spend lots of time getting information online without a nudge in any certain direction! Thank you! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From LSebree <@t> uwhealth.org Mon Oct 26 12:43:00 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Oct 26 12:43:06 2009 Subject: [Histonet] Looking for a "robust" EMA antibody Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF62E@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hello Histonetters, We are in the market for a nicely robust EMA that will readily stain meningiomas. We are currently using clone E29 but the formulation requires extremely long HIER and incubation times for meningiomas. We use Ventana instruments but all opinions are welcome. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From POWELL_SA <@t> mercer.edu Mon Oct 26 13:54:44 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Oct 26 13:55:03 2009 Subject: [Histonet] GSH 2010 meeting Message-ID: <9BF995BC0E47744E9673A41486E24EE222A533B4CE@MERCERMAIL.MercerU.local> The Georgia Society for Histotechnology Annual meeting will be held March 26-28, 2010 at Stone Mountain, the complete hotel information is listed below. GSH is now accepting abstracts for this meeting. The form to fill out may be downloaded from the NSH website, www.nsh.org or you may respond to this message and I will send one to you. Please mail completed forms to me as soon as possible. **** Deadline for abstracts is November 20, 2009. My address is: Shirley Powell, HT(ASCP)HTL, QIHC GSH Secretary Mercer University School of Medicine 1550 College Street Macon, GA 31207 **** Deadline for abstracts is November 20, 2009. Note:********If a session has been approved by NSH in the past, a complete abstract form is not necessary as long as the complete abstract is provided to GSH for the program printing, along with complete presenter information. Please contact me for more information if needed. GSH Annual Symposium will be at Evergreen Marriott(r) Conference Resort 4021 Lakeview Drive Stone Mountain, Georgia 30083 USA Toll-free: 1-888-670-2250 http://www.marriott.com/hotels/hotel-photos/ATLEG/?mktcmp=w_regionsite_atleg_x Room rates are $99 CALL FOR YOUR RESERVATIONS TODAY. BRING THE FAMILY TO ATLANTA AND ENJOY STONE MOUNTAIN, THE PARKS AND OTHER AREA ATTRACTIONS. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From HISTOBEACH <@t> aol.com Mon Oct 26 18:11:03 2009 From: HISTOBEACH <@t> aol.com (HISTOBEACH@aol.com) Date: Mon Oct 26 18:10:16 2009 Subject: [Histonet] Special Stain Systems Message-ID: Hi everyone, I'm a lab manager for a small private lab in Arizona. I wish not to use my work email, since I do not want vendors to contact me. I only wish to receive some information from users. Which special stain system do you use and are you happy with it? Why or why not? Could you please provide me some detailed info as to why you don't like or like the system? I'm looking for a good quality, reliable, consistent, timely efficient stainer. Is there one out there? I greatly appreciate your time to provide me with some feedback. Take care, Sally From katenjude <@t> yahoo.com Mon Oct 26 19:49:35 2009 From: katenjude <@t> yahoo.com (judith lopez) Date: Mon Oct 26 19:49:38 2009 Subject: [Histonet] (no subject) Message-ID: <255610.43962.qm@web65510.mail.ac4.yahoo.com> please delete me from histonet From Melanie.Black <@t> uct.ac.za Tue Oct 27 07:48:48 2009 From: Melanie.Black <@t> uct.ac.za (Melanie Black) Date: Tue Oct 27 07:50:29 2009 Subject: [Histonet] Green Fluorescent Protein Message-ID: Hi I am looking to demonstrate Green fluorescent protein in Para formaldehyde fixed, processed rat tissue. Apart from using an antibody against GFP, can the native GFP be detected? Gayle Callis, I believe you may be able to help me with this method. Thanks Melanie Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From leiker <@t> buffalo.edu Tue Oct 27 08:32:46 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Oct 27 08:32:49 2009 Subject: [Histonet] Green Fluorescent Protein In-Reply-To: References: Message-ID: <08E77701CAE8430EB7051F4E@CDYwxp1931.ad.med.buffalo.edu> The fluorescence itself will not be detectable, you would have to use an indirect means (like antibody, which you mentioned). That's just from my experience with it. Maybe Gayle Callis has a suggestion... Regards, Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. --On Tuesday, October 27, 2009 2:48 PM +0200 Melanie Black wrote: > Hi > > I am looking to demonstrate Green fluorescent protein in Para > formaldehyde fixed, processed rat tissue. Apart from using an antibody > against GFP, can the native GFP be detected? Gayle Callis, I believe you > may be able to help me with this method. > > Thanks > Melanie > > Melanie Black > 082 469 3352 > > Cardiovascular Research Unit > 3rd Floor; Chris Barnard Building > Medical School; > Observatory. 7925. > University of Cape Town. > South Africa. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From contact <@t> excaliburpathology.com Tue Oct 27 08:50:54 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Oct 27 08:51:02 2009 Subject: [Histonet] Green Fluorescent Protein In-Reply-To: References: Message-ID: <988880.60095.qm@web1116.biz.mail.sk1.yahoo.com> Hello, I routinely use Invitrogen's Rabbit anti-GFP on mouse and rat FFPE?tissue. Paula ________________________________ From: Melanie Black To: histonet@lists.utsouthwestern.edu Sent: Tue, October 27, 2009 7:48:48 AM Subject: [Histonet] Green Fluorescent Protein Hi I am looking to demonstrate Green fluorescent protein in Para formaldehyde fixed, processed rat tissue. Apart from using an antibody against GFP, can the native GFP be detected? Gayle Callis, I believe you may be able to help me with this method. Thanks Melanie Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janelle.pakan <@t> gmail.com Tue Oct 27 12:36:44 2009 From: janelle.pakan <@t> gmail.com (Janelle Pakan) Date: Tue Oct 27 12:37:09 2009 Subject: [Histonet] Problem wih FITC secondary "unbinding" after about 10 hours (using immunofluorescence) Message-ID: We are having a very strange problem with our FITC-conjugated secondary in double labeling immunofluorescence processing. We are processing for 3 different enzymes using a Rhodamine secondary and these seem to be fine throughout the process, but using 3 different markers (GFAP, MAP2, OX42) with a FITC conjugated secondary we see something very strange. The labeling looks really nice immediately after processing and mounting for up to 6-10 hours later, then when left in the fridge overnight, the next day the FITC (and only the FITC, the rhodamine is fine) seems to "unbind" and is all over the tissue creating a "background" type fluorescence. I have tried 4 different secondaries from 3 different companies (although they are all FITC conjugated - want to try a cy2 or alexafluor 488 but don't have any yet) and they all do they same thing - really nice immediately after processing, but cell labeling is "gone" by the next day and appears as specks all over the tissue sample. We are using 4%PFA fixed, floating rat brain sections (used three different brains so far and all had the same effect). These are the other things I checked: - used 3 different mounting media (Fluoromount-G, Elvanol, 90% glycerol): all had same effect. Leaving tissue overnight in PBS in fridge also had same effect, so figure it cant be anything with the mounting process. - used 3 different primaries and all have same effect - we use ovoid (BR0014G) PBS tablets (1 per 100ml, pH 7.3) - commercially available standard PBS tablets... should be okay?? Anyone else use this? Any help would be appreciated, or just to know if anyone has seen this before. I did immuno for 5 years in a different lab and never saw anything like this, but recently switched labs and had this problem immediately. I figure it must be something in the solutions I am using n the new lab, but I just cant fathom what it would be at this point. Thanks Dr. Janelle Pakan University of British Columbia Brain Research Centre 2211 Westbrook Mall Vancouver, BC Canada From leiker <@t> buffalo.edu Tue Oct 27 13:29:05 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Oct 27 13:29:14 2009 Subject: [Histonet] Problem wih FITC secondary "unbinding" after about 10 hours (using immunofluorescence) In-Reply-To: References: Message-ID: <4C0B3AF02358627571958E4E@CDYwxp1931.ad.med.buffalo.edu> We use a range of Alexa Fluors (including 488) all the time with either SlowFade Gold or ProLong Gold antifade mounting media on every primary antibody we use. We can still see the label a week or more later, especially if we store the slides at -20 C. Alexa Fluors are very bright and stable compared to traditional fluorophores like FITC, TRITC... Another consideration is are you doing confocal or epifluorescent imaging? Confocal will bleach your sample sooner. The Alexa Fluors and mounting media mentioned above are all available from Invitrogen at the following links (this is NOT a plug for Invitrogen, though it may appear that way; we are an independent lab!): Regards, Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. --On Tuesday, October 27, 2009 10:36 AM -0700 Janelle Pakan wrote: > We are having a very strange problem with our FITC-conjugated secondary in > double labeling immunofluorescence processing. We are processing for 3 > different enzymes using a Rhodamine secondary and these seem to be fine > throughout the process, but using 3 different markers (GFAP, MAP2, OX42) > with a FITC conjugated secondary we see something very strange. The > labeling looks really nice immediately after processing and mounting for > up to 6-10 hours later, then when left in the fridge overnight, the next > day the FITC (and only the FITC, the rhodamine is fine) seems to "unbind" > and is all over the tissue creating a "background" type fluorescence. > > I have tried 4 different secondaries from 3 different companies (although > they are all FITC conjugated - want to try a cy2 or alexafluor 488 but > don't have any yet) and they all do they same thing - really nice > immediately after processing, but cell labeling is "gone" by the next day > and appears as specks all over the tissue sample. > > We are using 4%PFA fixed, floating rat brain sections (used three > different brains so far and all had the same effect). > > These are the other things I checked: > - used 3 different mounting media (Fluoromount-G, Elvanol, 90% glycerol): > all had same effect. Leaving tissue overnight in PBS in fridge also had > same effect, so figure it cant be anything with the mounting process. - > used 3 different primaries and all have same effect > - we use ovoid (BR0014G) PBS tablets (1 per 100ml, pH 7.3) - commercially > available standard PBS tablets... should be okay?? Anyone else use this? > > Any help would be appreciated, or just to know if anyone has seen this > before. I did immuno for 5 years in a different lab and never saw anything > like this, but recently switched labs and had this problem immediately. I > figure it must be something in the solutions I am using n the new lab, > but I just cant fathom what it would be at this point. > > Thanks > > Dr. Janelle Pakan > University of British Columbia > Brain Research Centre > 2211 Westbrook Mall > Vancouver, BC > Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From anonwums1 <@t> gmail.com Tue Oct 27 13:41:52 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Oct 27 13:41:57 2009 Subject: [Histonet] Green Fluorescent Protein In-Reply-To: <988880.60095.qm@web1116.biz.mail.sk1.yahoo.com> References: <988880.60095.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: <858249120910271141x39919f23qacc96ca44e230b79@mail.gmail.com> At the suggestion from someone on Histonet, I've been using Abcam's chicken anti-GFP. It's worked great on mouse tissue fixed with paraformaldehyde, Zn buffered formalin, or 10% neutral buffered formalin. Even though Abcam suggests that you antigen retrieve, I've found it works just as well without it. As for viewing GFP without any antibody, you apparently can, at least in bone. See http://skeletalbiology.uchc.edu/30_ResearchProgram/304_gap/index.htm for a really detailed discussion. I've tried before and it hasn't worked... I think you need really high quality optics and filter cubes. Adam On Tue, Oct 27, 2009 at 8:50 AM, Paula Pierce < contact@excaliburpathology.com> wrote: > Hello, > > I routinely use Invitrogen's Rabbit anti-GFP on mouse and rat FFPE tissue. > > Paula > > > > > ________________________________ > From: Melanie Black > To: histonet@lists.utsouthwestern.edu > Sent: Tue, October 27, 2009 7:48:48 AM > Subject: [Histonet] Green Fluorescent Protein > > Hi > > I am looking to demonstrate Green fluorescent protein in Para formaldehyde > fixed, processed rat tissue. Apart from using an antibody against GFP, can > the native GFP be detected? Gayle Callis, I believe you may be able to help > me with this method. > > Thanks > Melanie > > Melanie Black > 082 469 3352 > > Cardiovascular Research Unit > 3rd Floor; Chris Barnard Building > Medical School; > Observatory. 7925. > University of Cape Town. > South Africa. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carl.hobbs <@t> kcl.ac.uk Tue Oct 27 13:46:26 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Tue Oct 27 13:46:39 2009 Subject: [Histonet] Green Fluorescent Protein Message-ID: <11D9615B89C10747B1C985966A63D7CA2C3F25A26D@KCL-MAIL04.kclad.ds.kcl.ac.uk> GFP can be detected directly in FFPwax sections if the expression level is high enough. Yo can easliy check this, using one section before you immunoprobe. Sure, Ab detection will guarantee you a strong signal but, again, if the exression of the protein is high enough. I have come across several tissues where I can't even detect GFP using an Ab. I agree that both Invitrogen's ( mouse A11120 and rabbit A11122 ) anti GFP Abs are very good. So also is Abcam's chicken anti GFP ab13970 : for me it gives the best signal but, you'd specifically need to buy an anti Chicken IgY conjugate. If you care to browse in the Image gallery here: http://www.immunoportal.com/index.php, there are some images. good luck! Carl From sbreeden <@t> nmda.nmsu.edu Tue Oct 27 13:52:15 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Oct 27 13:52:19 2009 Subject: [Histonet] Quick help with fixative? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BD8@nmdamailsvr.nmda.ad.nmsu.edu> Why would lymph nodes for AFB be submitted in saturated sodium borate? I'm cutting in now and want to know why this would be! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From janelle.pakan <@t> gmail.com Tue Oct 27 14:05:19 2009 From: janelle.pakan <@t> gmail.com (Janelle Pakan) Date: Tue Oct 27 14:05:43 2009 Subject: [Histonet] Problem wih FITC secondary "unbinding" after about 10 hours (using immunofluorescence) In-Reply-To: <4C0B3AF02358627571958E4E@CDYwxp1931.ad.med.buffalo.edu> References: <4C0B3AF02358627571958E4E@CDYwxp1931.ad.med.buffalo.edu> Message-ID: Thanks Merced, We are doing confocal, but the problem doesnt seem to be bleaching - the fluorescence is still there, it is just no longer concentrated within the cells we are staining for, but rather dispersed all over the tissue. Almost like it "leaked" out of the cells we were staining. As I mentioned, I dont think it can be the mounting media, as when we left the tissue in PBS overnight without mounting it, the signal was still gone the next day. Must be some sort of solution problem... maybe salts, pH, fixative?? But we are using standard protocols and comercially available PBS. I am stumped... Dr. Janelle Pakan University of British Columbia Brain Research Centre 2211 Westbrook Mall Vancouver, BC Canada On Tue, Oct 27, 2009 at 11:29 AM, Merced M Leiker wrote: > We use a range of Alexa Fluors (including 488) all the time with either > SlowFade Gold or ProLong Gold antifade mounting media on every primary > antibody we use. We can still see the label a week or more later, especially > if we store the slides at -20 C. > > Alexa Fluors are very bright and stable compared to traditional > fluorophores like FITC, TRITC... > > Another consideration is are you doing confocal or epifluorescent imaging? > Confocal will bleach your sample sooner. > > The Alexa Fluors and mounting media mentioned above are all available from > Invitrogen at the following links (this is NOT a plug for Invitrogen, though > it may appear that way; we are an independent lab!): > > < > http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/alexa-fluor.html > > > < > http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/SlowFade-Gold.html > > > < > http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/ProLong-Antifades-Brand-Page.html > > > > Regards, > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > --On Tuesday, October 27, 2009 10:36 AM -0700 Janelle Pakan < > janelle.pakan@gmail.com> wrote: > > We are having a very strange problem with our FITC-conjugated secondary in >> double labeling immunofluorescence processing. We are processing for 3 >> different enzymes using a Rhodamine secondary and these seem to be fine >> throughout the process, but using 3 different markers (GFAP, MAP2, OX42) >> with a FITC conjugated secondary we see something very strange. The >> labeling looks really nice immediately after processing and mounting for >> up to 6-10 hours later, then when left in the fridge overnight, the next >> day the FITC (and only the FITC, the rhodamine is fine) seems to "unbind" >> and is all over the tissue creating a "background" type fluorescence. >> >> I have tried 4 different secondaries from 3 different companies (although >> they are all FITC conjugated - want to try a cy2 or alexafluor 488 but >> don't have any yet) and they all do they same thing - really nice >> immediately after processing, but cell labeling is "gone" by the next day >> and appears as specks all over the tissue sample. >> >> We are using 4%PFA fixed, floating rat brain sections (used three >> different brains so far and all had the same effect). >> >> These are the other things I checked: >> - used 3 different mounting media (Fluoromount-G, Elvanol, 90% glycerol): >> all had same effect. Leaving tissue overnight in PBS in fridge also had >> same effect, so figure it cant be anything with the mounting process. - >> used 3 different primaries and all have same effect >> - we use ovoid (BR0014G) PBS tablets (1 per 100ml, pH 7.3) - commercially >> available standard PBS tablets... should be okay?? Anyone else use this? >> >> Any help would be appreciated, or just to know if anyone has seen this >> before. I did immuno for 5 years in a different lab and never saw anything >> like this, but recently switched labs and had this problem immediately. I >> figure it must be something in the solutions I am using n the new lab, >> but I just cant fathom what it would be at this point. >> >> Thanks >> >> Dr. Janelle Pakan >> University of British Columbia >> Brain Research Centre >> 2211 Westbrook Mall >> Vancouver, BC >> Canada >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > From sbreeden <@t> nmda.nmsu.edu Tue Oct 27 15:00:26 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Oct 27 15:00:30 2009 Subject: [Histonet] Sodium Borate question resolved Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46BDD@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to all who responded to my sodium borate question. I've been helped and am Internally Grateful (whatever...). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From jcox90 <@t> yahoo.com Tue Oct 27 15:05:04 2009 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue Oct 27 15:05:07 2009 Subject: [Histonet] Equipment maintenance companies in Phoenix AZ? Message-ID: <365348.39505.qm@web56808.mail.re3.yahoo.com> Hi Netters,Does anyone know of a good histology equipment?maintenance?company in phoenix AZ? Annual maintenance is due so need to schedule soon. Thanks in advance.. Jill Cox HT (ASCP) ? ? ? ? From sfeher <@t> CMC-NH.ORG Tue Oct 27 17:11:47 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Tue Oct 27 17:36:00 2009 Subject: [Histonet] HT (ASCP) Position Available Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB2171@exchange.cmc-nh.org> I have a full time HT (ASCP) position available at a mid-sized Medical Center in Manchester, NH. This is a hospital based start up opportunity in a brand new lab that starts Dec 1. This is a day shift position. If you are interested apply at: http://www.catholicmedicalcenter.org/Career/JobSearch.aspx or email me sfeher@cmc-nh.org Thanks, Steve Feher Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From mgdelaware <@t> comcast.net Tue Oct 27 18:44:58 2009 From: mgdelaware <@t> comcast.net (marian powers) Date: Tue Oct 27 18:45:02 2009 Subject: [Histonet] Unsubscribe Message-ID: Unsubscribe From jshelley <@t> burnham.org Wed Oct 28 09:58:15 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Wed Oct 28 09:58:22 2009 Subject: [Histonet] Alternative to GLUOX Message-ID: Hi Histonetters, I have a protocol that requires GLUOX but I do not have the chemicals to make it at this present time. Is there an alternative or can I just use H2O2 at a weaker concentration. The antibody is a home-brew RNF5 on frozen muscle sections. Any thoughts would be appreciated. John From gayle.callis <@t> bresnan.net Wed Oct 28 10:39:36 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 28 10:39:49 2009 Subject: [Histonet] RE: alternative to Glucose oxidase (GLUOX) peroxidase block Message-ID: <000701ca57e4$dbb82980$93287c80$@callis@bresnan.net> You wrote: I have a protocol that requires GLUOX but I do not have the chemicals to make it at this present time. Is there an alternative or can I just use H2O2 at a weaker concentration. The antibody is a home-brew RNF5 on frozen muscle sections. Any thoughts would be appreciated. ************************************************************************* If you are fixing the muscle frozen sections with a solvent e.g. acetone, then you can try using a very weak solution of hydrogen peroxide in buffer. Try 0.1% Hydrogen peroxide, and if that eats the sections off the slides (you will see bubbles coming off the top of the frozen section, particularly if the tissue has lots of red blood cells, endogenous peroxidase. You can always cut the H2O2 concentration down, or even try slightly higher concentration (0.2 to 0.3%) This has been discussed in the past on Histonet, and can be found in Histonet Archives. I will send you a weak endogenous peroxidase blocker under separate private email This is well known and found in the literature. The solution has low concentration H2O2 and sodium azide in buffer. It is a nice inhouse protocol. You might have to apply it twice, 10 minutes then drain and do the block again for another 10 minutes but it does NOT eat you sections off the slide. The alternative is use Alkaline phosphatase instead of HRP then a peroxidase block is NOT needed. There are some sensitive AP chromogens that are as good DAB. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT 59715 From JCBRITTON <@t> Cheshire-Med.COM Wed Oct 28 11:20:05 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Oct 28 11:20:14 2009 Subject: [Histonet] IHC Patient Negatives Message-ID: Hi All, I have an issue with my computer, so I cannot see past the little paragraphs on the Histonet archives. Sorry! This question is probably asked many, many times. When we run our IHC's we always have a patient negative slide to go with our case. We run everything accept Herceptin on the Bond. When running a patient's IHC on the bench and on the Bond we use a negative patient control for both. (We run a test control on all antibodies also) If we are running a double stain IHC also we run another negative patient control for that stain also. The debate we are having is, if the next day you run another IHC on the same patient using the same DAB define kit, should we be running another negative patient control? Thanks for your help! Josie Britton HT Cheshire Medical Center CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From LSebree <@t> uwhealth.org Wed Oct 28 11:28:21 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Oct 28 11:28:28 2009 Subject: [Histonet] IHC Patient Negatives In-Reply-To: Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF63A@UWHC-MAIL01.uwhis.hosp.wisc.edu> Yes. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Wednesday, October 28, 2009 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Patient Negatives Hi All, I have an issue with my computer, so I cannot see past the little paragraphs on the Histonet archives. Sorry! This question is probably asked many, many times. When we run our IHC's we always have a patient negative slide to go with our case. We run everything accept Herceptin on the Bond. When running a patient's IHC on the bench and on the Bond we use a negative patient control for both. (We run a test control on all antibodies also) If we are running a double stain IHC also we run another negative patient control for that stain also. The debate we are having is, if the next day you run another IHC on the same patient using the same DAB define kit, should we be running another negative patient control? Thanks for your help! Josie Britton HT Cheshire Medical Center CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Wed Oct 28 11:33:45 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Oct 28 11:33:49 2009 Subject: [Histonet] Job In GA (Private Lab) Message-ID: Allied Search Partners is now accepting resumes for our clients of Peachtree City, GA. We are accepting the following resumes for permanent/direct hire positions: Histotechnologists/Histotechnicians Shifts: Day Shift Location: Private Lab Requirements: ASCP preferred. Looking for a tech with at least 2 years of experience. Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* Upon resume submission one of our recruiters will call you for an initial phone interview. No resume will be submitted before an initial phone interview with you. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From alyssa <@t> alliedsearchpartners.com Wed Oct 28 11:57:17 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Oct 28 11:57:21 2009 Subject: [Histonet] Managerial HT/HTL Job In MA Message-ID: ASP has the following histology opening at one of our client facilities in Burlington, MA area. Position: Manager of Histology Experience: Senior Histotechnician/Histotechnologist, Histology Supervisor, Histology Manager. Day shift: Full time ASCP required, BS degree preferred, experience as a supervisor OR Senior Histotechnician/Histotechnologist *Sign on Bonus Offered Through Allied Search Partners* *Please send resume for prescreening consideration to: Alyssa@alliedsearchpartners.com All inquiries are kept confidential. Upon receiving resume one of our recruiters will contact you for a phone screen. No resume will be sent to client without directly speaking to you first. *Be sure to visit us on the web* www.alliedsearchpartners.com $Cash Bonus$ for Referrals -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From gu.lang <@t> gmx.at Wed Oct 28 12:00:47 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 28 12:00:54 2009 Subject: [Histonet] tisssue preparation for AFM Message-ID: Hi all! I was asked for advice, but have to give the question further to you. We look for the best preparation of cornea for the atomic force microscope. A student wants to investigate the difference before and after the treatment of the cornea with a crosslinking drug. So any crosslinking fixative would bring artificial crosslinks and cannot be used. I think, that coagulating fixatives like ethanol, aceton etc. would also lead to artificial changes of the proteinstructure. The students wants to demonstrate the crosslinks of collagenfibers in cornea with the AFM. And the cornea should be seen as cross section to reach the interesting area. We will try to make frozen sections, but I doubt, that they will have the necessary quality and stability for AFM. So any tipp or hint is highly appreciated. Gudrun Lang From bridget.maryott <@t> ventana.roche.com Wed Oct 28 12:41:58 2009 From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget) Date: Wed Oct 28 12:42:51 2009 Subject: [Histonet] RE: Equipment maintenance companies in Phoenix AZ? Message-ID: <1975224336006344AA8326CAEF913DB008431B48@rnumsem704.nala.roche.com> GTI Microsystems 1022 N Stadem Dr Tempe, AZ 85281 480.968.1930 Ken Alger is great and I wouldn't let anyone else touch my microtome. Message: 8 Date: Tue, 27 Oct 2009 13:05:04 -0700 (PDT) From: Jill Cox Subject: [Histonet] Equipment maintenance companies in Phoenix AZ? To: histonet@lists.utsouthwestern.edu Message-ID: <365348.39505.qm@web56808.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Netters,Does anyone know of a good histology equipment?maintenance?company in phoenix AZ? Annual maintenance is due so need to schedule soon. Thanks in advance.. Jill Cox HT (ASCP) ? ? ? Bridget Maryott Advanced Staining Ventana Medical Systems, Inc. a member of the Roche Group 1910 E. Innovation Park Drive Tucson, Arizona 85755 Tel: +1 520 229 4022 mailto: bridget.maryott@ventana.roche.com Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. From ploykasek <@t> phenopath.com Wed Oct 28 13:56:36 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Oct 28 13:56:46 2009 Subject: [Histonet] IHC Patient Negatives In-Reply-To: Message-ID: Well, I hope I don?t start a controversy, but here's my 2 cents worth. I don't think you should run another negative. If you are going to have any staining due to intrinsic tissue elements reacting with your detection, you will see it the first day. I am of the opinion that in most cases too many negative controls are run. You are wasting precious patient material. With today's polymer detection systems, there is very little background staining. If you are doing additional testing on a patient using a different type of detection, I do think you should run another negative. Please don't flame me too badly - I'm having a bad day! And it is only my 2 cents worth! Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA > Hi All, > > > > I have an issue with my computer, so I cannot see past the little > paragraphs on the Histonet archives. Sorry! This question is probably > asked many, many times. > > > > When we run our IHC's we always have a patient negative slide to go with > our case. We run everything accept Herceptin on the Bond. When running > a patient's IHC on the bench and on the Bond we use a negative patient > control for both. (We run a test control on all antibodies also) If we > are running a double stain IHC also we run another negative patient > control for that stain also. The debate we are having is, if the next > day you run another IHC on the same patient using the same DAB define > kit, should we be running another negative patient control? > > > > Thanks for your help! > > > > Josie Britton HT > > Cheshire Medical Center > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is > for the sole use of the intended recipients and may contain confidential and > privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mward <@t> wfubmc.edu Wed Oct 28 14:21:22 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Oct 28 14:28:28 2009 Subject: [Histonet] IHC Patient Negatives In-Reply-To: References: Message-ID: <61135F0455D33347B5AAE209B903A30429D351CD@EXCHVS2.medctr.ad.wfubmc.edu> Patti, I agree with you. We do not run another negative control under the circumstances you and Josie outlined. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 28, 2009 2:57 PM To: Josie Britton; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC Patient Negatives Well, I hope I don?t start a controversy, but here's my 2 cents worth. I don't think you should run another negative. If you are going to have any staining due to intrinsic tissue elements reacting with your detection, you will see it the first day. I am of the opinion that in most cases too many negative controls are run. You are wasting precious patient material. With today's polymer detection systems, there is very little background staining. If you are doing additional testing on a patient using a different type of detection, I do think you should run another negative. Please don't flame me too badly - I'm having a bad day! And it is only my 2 cents worth! Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA > Hi All, > > > > I have an issue with my computer, so I cannot see past the little > paragraphs on the Histonet archives. Sorry! This question is > probably asked many, many times. > > > > When we run our IHC's we always have a patient negative slide to go > with our case. We run everything accept Herceptin on the Bond. When > running a patient's IHC on the bench and on the Bond we use a negative > patient control for both. (We run a test control on all antibodies > also) If we are running a double stain IHC also we run another > negative patient control for that stain also. The debate we are > having is, if the next day you run another IHC on the same patient > using the same DAB define kit, should we be running another negative patient control? > > > > Thanks for your help! > > > > Josie Britton HT > > Cheshire Medical Center > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Oct 28 14:53:13 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Oct 28 14:53:18 2009 Subject: [Histonet] circle slides for double cyto spin Message-ID: Good afternoon everyone, Does anyone know of a vendor that can supply us with circle slides for a double cyto spin. Our cyto funnels are off center - meaning that the lower circle is more on the left side of the slide, and the upper circle is more to the right side. Vendors are welcome to reply. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From KGroeger <@t> USLABS.net Wed Oct 28 14:53:47 2009 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Wed Oct 28 14:53:52 2009 Subject: [Histonet] IHC Patient Negatives In-Reply-To: <61135F0455D33347B5AAE209B903A30429D351CD@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30429D351CD@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: I totally agree with you. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Wednesday, October 28, 2009 12:21 PM To: Patti Loykasek; Josie Britton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC Patient Negatives Patti, I agree with you. We do not run another negative control under the circumstances you and Josie outlined. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, October 28, 2009 2:57 PM To: Josie Britton; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC Patient Negatives Well, I hope I don?t start a controversy, but here's my 2 cents worth. I don't think you should run another negative. If you are going to have any staining due to intrinsic tissue elements reacting with your detection, you will see it the first day. I am of the opinion that in most cases too many negative controls are run. You are wasting precious patient material. With today's polymer detection systems, there is very little background staining. If you are doing additional testing on a patient using a different type of detection, I do think you should run another negative. Please don't flame me too badly - I'm having a bad day! And it is only my 2 cents worth! Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA > Hi All, > > > > I have an issue with my computer, so I cannot see past the little > paragraphs on the Histonet archives. Sorry! This question is > probably asked many, many times. > > > > When we run our IHC's we always have a patient negative slide to go > with our case. We run everything accept Herceptin on the Bond. When > running a patient's IHC on the bench and on the Bond we use a negative > patient control for both. (We run a test control on all antibodies > also) If we are running a double stain IHC also we run another > negative patient control for that stain also. The debate we are > having is, if the next day you run another IHC on the same patient > using the same DAB define kit, should we be running another negative patient control? > > > > Thanks for your help! > > > > Josie Britton HT > > Cheshire Medical Center > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From histosearch <@t> gmail.com Wed Oct 28 15:11:05 2009 From: histosearch <@t> gmail.com (HistoLab) Date: Wed Oct 28 15:11:09 2009 Subject: [Histonet] circle slides for double cyto spin Message-ID: <521c6d260910281311q3ba69458re6d7153b3c16f88b@mail.gmail.com> Toni, I would be happy to give you a quote for the double cyto spin slides. Please contact with the with the info provided below: Matthew Semovoski Gorilla Scientific Corporation www.GorillaScientific.com Email: matt@gorillascientific.com 1-866-435-4977 From stzuckerman <@t> gmail.com Wed Oct 28 15:16:28 2009 From: stzuckerman <@t> gmail.com (Sean Zuckerman) Date: Wed Oct 28 15:16:33 2009 Subject: [Histonet] Question about Goldner's Trichrome collagen staining Message-ID: <257c8ffa0910281316p7a38dd91x7d35aa09d717e7af@mail.gmail.com> Dear Histonetters, I am attempting to use Goldner?s Trichrome to demonstrate bony tissue formation in human mesenchymal stem cell pellet culture via endochondral ossification. Based on morphology I see something resembling bony tissue on the outer surface of the pellets. My sections take Solution A up rapidly (~1 min) but do not seem to de-stain anywhere near as rapidly (15-20 min). And I?ve found that it takes ~15-20 min in Solution D to obtain reasonable staining of any collagen. I have found one reference to extended staining times for Solution C (Orange G/PMA) & Solution D (Light Green) on Histonet, but the reference was in regards to 15 um sections of ground MMA sections and I am dealing with 5 um paraffin-embedded sections. I have also substituted Fast Green FCF for Light Green in Solution D. Am I right in thinking these times for Soln?s C & D are too long? Should I use the variation with azophloxine? Any advice is greatly appreciated. Thank you for your time & assistance. Sincerely, Sean Zuckerman, PhD Postdoctoral Research Fellow Department of Biomedical Engineering Case Western Reserve University Extended incubation in Soln?s C & D: http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-February/035721.html I am following the protocol from Gray?s Microtomist?s Formulary (1954): Weigert?s hematoxylin - 15 min Rinse in tap water followed by Solution B Solution A ? 5 min Rinse briefly in Solution B Solution C ? till collagen de-stains Rinse briefly in Solution B Solution D ? 5 min Solution B ? 5 min Dehydrate & mount Solution A: 100 ml water 0.07 g Ponceau Xylidine 0.03 g Acid fuchsin 0.2 ml Acetic acid, glacial Solution B: 99 ml water 1 ml Acetic acid, glacial Solution C: 100 ml water 4 g phosphomolybdic acid 2 g Orange G Solution D: 100 ml water 0.2 g Fast Green FCF (subbed for Light Green) 0.2 ml Acetic acid, glacial From Nancy.Herman <@t> inspection.gc.ca Wed Oct 28 15:19:59 2009 From: Nancy.Herman <@t> inspection.gc.ca (Nancy Herman) Date: Wed Oct 28 15:20:24 2009 Subject: [Histonet] GFAP IHC Message-ID: <20091028T141959Z_6DEB00150000@inspection.gc.ca> We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and using Envision+ HRP Rabbit detection system. When using this antibody we see a brown color on the slide itself. The staining was intense and quite uneven. We have diluted the anitbody to 1:10 but there is still the brown slide. The staining is not quite as uneven but appears more washed out. We don't see this with any of our other antibodies we are using and our no primary controls for GFAP are clean. Any advice or explanations for the slides picking up the brown color or improving GFAP IHC on bovine tissue? Thanks Nancy Herman CFIA Lethbridge Laboratory Lethbridge, Alberta From Jackie.O'Connor <@t> abbott.com Wed Oct 28 15:35:35 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Oct 28 15:36:02 2009 Subject: [Histonet] Canine endothelial antibody In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190FDB2171@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF190FDB2171@exchange.cmc-nh.org> Message-ID: A week or so ago I thought I saw a post about an antibody that works for canine endothelial cells, but I can't find it in the archives - was I just dreaming, because I need this so bad? I've tried Factor VIII and a CD34 on FFPE, but with poor results. Any help would be most appreciated. Thanks, Jackie O' From karla <@t> dermatopathologynorthwest.com Wed Oct 28 15:56:04 2009 From: karla <@t> dermatopathologynorthwest.com (karla@dermatopathologynorthwest.com) Date: Wed Oct 28 15:56:08 2009 Subject: [Histonet] ihc billing? 2 specs on same slide Message-ID: <4A4D6F7A00018486@relay2.msp.eschelon.com> Hello Histonetters Could someone help me out on this? Is it correct to bill for two immuno charges if tissue from blocks "A" and "B" (separately designated sites) are placed on the same slide (small specs) clearly identified on the same slide and stained with one antibody? Any help with information regarding placing two separate tissue sites/blocks on the same slide would be helpful. Thanks, Karla Carlmas From CIngles <@t> uwhealth.org Wed Oct 28 16:15:47 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Oct 28 16:16:42 2009 Subject: [Histonet] Plants & windows in lab References: <5A2BD13465E061429D6455C8D6B40E390987A86997@IBMB7Exchange.digestivespecialists.com> Message-ID: I didn't think they had sun in Seattle. :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Blazek, Linda Sent: Fri 10/23/2009 10:39 AM To: histonet Subject: RE: [Histonet] Plants & windows in lab Don't go to the web site. It has a picture of the water and boats! It will just drive you batty! -----Original Message----- From afleming <@t> mednet.ucla.edu Wed Oct 28 17:33:58 2009 From: afleming <@t> mednet.ucla.edu (Alice Fleming) Date: Wed Oct 28 17:34:09 2009 Subject: [Histonet] imaging organ cultures Message-ID: <9FB24CE8-0ED0-4E0E-B98E-CCF5ED9672E6@mednet.ucla.edu> Hi, I am doing whole mount IHCs on embryonic organ cultures and imaging them on a confocal microscope. The IHCs are going well, but I?m having trouble making the tissue stay in a specific position for imaging. I have been putting the tissue in the wells of depression slides, adding about 30uL of Vectashield mounting medium, then coverslipping. This works fine, except that I have little control over the final position of the tissue?as I put the coverslip down, the tissue often moves. Any suggestions would be welcome! Is there, for example, some kind of optically neutral agarose that I could spot around the tissue before adding the Vectashield and coverslip?? Or?other ideas?? Thanks, Alice Alice Fleming Department of Human Genetics University of California Los Angeles Los Angeles, CA 90095 310-267-2456 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From portera <@t> msu.edu Thu Oct 29 07:11:33 2009 From: portera <@t> msu.edu (Amy Porter) Date: Thu Oct 29 07:11:39 2009 Subject: [Histonet] Canine endothelial antibody References: <73A7ED895EE0C24D9267ED814911DF190FDB2171@exchange.cmc-nh.org> Message-ID: <312A238ED6B14F049DDB8AB272BD4A7F@histolab> We use Dako Von Willebrand Factor VIII in Rabbit on multiple species and it works fabulously. Enzyme pretreatment with 0.03% pronase e for 10 mins at 37C / Primary at 1:200 to 1:500 depending on the species being stained. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, October 28, 2009 4:35 PM Subject: [Histonet] Canine endothelial antibody >A week or so ago I thought I saw a post about an antibody that works for > canine endothelial cells, but I can't find it in the archives - was I just > dreaming, because I need this so bad? I've tried Factor VIII and a CD34 > on FFPE, but with poor results. Any help would be most appreciated. > Thanks, > Jackie O' > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From portera <@t> msu.edu Thu Oct 29 07:14:53 2009 From: portera <@t> msu.edu (Amy Porter) Date: Thu Oct 29 07:14:54 2009 Subject: [Histonet] GFAP IHC References: <20091028T141959Z_6DEB00150000@inspection.gc.ca> Message-ID: <4B13F06368D543EC92DE206E438B4BBC@histolab> If I remember correctly Dako's GFAP is a bovine immunogen (bovine spinal cord) - that is probably what is causing the problem. You may want to look for GFAP where the cow is not the immunogen / try running another brain from a different species and see if you get the same problem. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Nancy Herman" To: Sent: Wednesday, October 28, 2009 4:19 PM Subject: [Histonet] GFAP IHC > We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and > using Envision+ HRP Rabbit detection system. When using this antibody we > see a brown color on the slide itself. The staining was intense and quite > uneven. We have diluted the anitbody to 1:10 but there is still the brown > slide. The staining is not quite as uneven but appears more washed out. > We don't see this with any of our other antibodies we are using and our no > primary controls for GFAP are clean. Any advice or explanations for the > slides picking up the brown color or improving GFAP IHC on bovine tissue? > Thanks > > Nancy Herman > CFIA Lethbridge Laboratory > Lethbridge, Alberta -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Thu Oct 29 09:29:15 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Oct 29 09:29:20 2009 Subject: [Histonet] GFAP IHC References: <20091028T141959Z_6DEB00150000@inspection.gc.ca> Message-ID: Nancy, I don't use the ready-to-use GFAP from Dako, but I do use the concentrated form of GFAP (it is also a rabbit anti-cow GFAP.) It's worked successfully in dog, cat, pig, cow, horse, goat, sheep, deer, eagle, mouse, rat, gerbil, guinea pig, hamster, monkey, ferret, and kangaroo with no background problems whatsoever. The EnVision+HRP Rabbit will only bind to the rabbit IgG, not the cow IgG, so that is not the source of your background. I would suspect that if you're getting brown staining on the slide itself, your antibody is much much too concentrated. My working concentration calculates out to be 4 ug/ml of Ab. You might see if your ready-to-use preparation has a concentration listed, and then work from there to approximate a 4 ug/ml concentration with diluting it out. If that doesn't work... buy the concentrated stock form and optimize it for your tissues. In my lab, it requires absolutely no tissue pretreatment, and the stain is always strong, clear and clean. Good Luck! Best regards, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Nancy Herman" To: Sent: Wednesday, October 28, 2009 3:19 PM Subject: [Histonet] GFAP IHC > We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and > using Envision+ HRP Rabbit detection system. When using this antibody we > see a brown color on the slide itself. The staining was intense and quite > uneven. We have diluted the anitbody to 1:10 but there is still the brown > slide. The staining is not quite as uneven but appears more washed out. > We don't see this with any of our other antibodies we are using and our no > primary controls for GFAP are clean. Any advice or explanations for the > slides picking up the brown color or improving GFAP IHC on bovine tissue? > Thanks > > Nancy Herman > CFIA Lethbridge Laboratory > Lethbridge, Alberta -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Thu Oct 29 09:40:06 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Oct 29 09:40:10 2009 Subject: [Histonet] Canine endothelial antibody References: <73A7ED895EE0C24D9267ED814911DF190FDB2171@exchange.cmc-nh.org> Message-ID: Jackie, Dako's Factor VIII (von Willebrand Factor), cat. #A0082 works for me on canine tissue (and cat, pig, cow, horse, sheep, chicken, mouse, etc.). I use Proteinase K enzyme digestion pretreatment for 5' at room temperature; primary rabbit Ab incubation 30', EnVision+/HRP anti-Rabbit 30' (with 4% normal dog serum added per volume for blocking), AEC 10'. Hope this helps, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, October 28, 2009 3:35 PM Subject: [Histonet] Canine endothelial antibody >A week or so ago I thought I saw a post about an antibody that works for > canine endothelial cells, but I can't find it in the archives - was I just > dreaming, because I need this so bad? I've tried Factor VIII and a CD34 > on FFPE, but with poor results. Any help would be most appreciated. > Thanks, > Jackie O' > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Thu Oct 29 09:54:39 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Oct 29 09:54:44 2009 Subject: [Histonet] RELIA Histology Job Alert for Supervisors, Managers Techs and PAs. Hot Hot Hot Jobs!! Message-ID: Hi Histonetters!! I hope everyone is having a great day and ramping up for a great weekend. Any plans for Halloween? I have some great opportunities that I want to tell you about. All of these opportunities are permanent full time positions with some of my best clients who are offering excellent compensation and benefits and in most cases relocation and or sign on bonuses and the newest and hottest positions have an * Histology Management Histology Manager - Charlotte, NC* Pathology Supervisor - Fresno, CA* Histology Supervisor - Los Angeles, CA Pathology Supervisor - Cape Cod, MA Pathology Manager - Portland, OR Histology Supervisor - Austin, TX* Histology Supervisor - Spokane, WA Pathology Assistant PA - Charlotte NC* Histotechnicians/Histotechnologists Lead Histotech - Tucson area, AZ* Grossing Histotech - Atlanta, GA (night shift)* Histotech - Pittsburgh Histotech - Orange/Rockland, Cty, NY Histotech - Plattsburgh, NY Histotech - Largo, FL part time afternoons M-F If you or anyone you know might be interested in hearing more about any of these opportunities please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From billodonnell <@t> catholichealth.net Thu Oct 29 10:19:14 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Oct 29 10:20:05 2009 Subject: [Histonet] Celerus Wave Message-ID: Good day, Is anyuone using the Celerus Wave rapid IHC system? Would appriciate any feedback. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From YANG-MING_YANG <@t> NYMC.EDU Tue Oct 27 13:44:06 2009 From: YANG-MING_YANG <@t> NYMC.EDU (Yang, YangMing) Date: Thu Oct 29 10:25:01 2009 Subject: [Histonet] Tissue-Tek II Manual Message-ID: <3BF57DCE362ECA4BAB679E567091DB24169D3EF326@mail3.nymc.edu> Hello, I saw your email from internet. We have an old Cryostat (model: Tissue-Tek II 4553 from Miles) without the instruction manual. It will be very grateful if you already found one, and can shear with us. If you have it could you email me a copy? We are trying to fix our Cryostat. Best regards, Yang-Ming Yang Dept of Physiology New York Medical College BSB, room 629 From abijag76 <@t> yahoo.co.in Thu Oct 29 11:19:56 2009 From: abijag76 <@t> yahoo.co.in (abi jag) Date: Thu Oct 29 11:20:03 2009 Subject: [Histonet] Import of staining reagents(richard allan/microm, now thermo) Message-ID: <336106.60837.qm@web95110.mail.in2.yahoo.com> Hello histonetters, This is with regard to import of Richard allan(microm, now thermo)staining reagents from US to India. After facing several problems from stains manufactured by local manufacturers, we thought of switching over to RA stains. The local distributor here is telling that these reagents(we are planning for procuring hematoxylin,eosin,bluing reagent and clarifier)are highly hazardous and need special packing(itself require 4000 US $). Anybody having any sort of experience about this and having idea about how RA stains can be transported/used in other parts of the world. Thanks for all your help abijag Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew From MSHERWOOD <@t> PARTNERS.ORG Thu Oct 29 12:17:16 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Oct 29 12:17:32 2009 Subject: [Histonet] Import of staining reagents(richard allan/microm, now thermo) In-Reply-To: <336106.60837.qm@web95110.mail.in2.yahoo.com> References: <336106.60837.qm@web95110.mail.in2.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23D9C@PHSXMB30.partners.org> We order RA Hematoxlylin 2 and Eosin-Y from Fisher. Haven't heard anything about additional cost, etc. Peg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abi jag Sent: Thursday, October 29, 2009 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Import of staining reagents(richard allan/microm,now thermo) Hello histonetters, This is with regard to import of Richard allan(microm, now thermo)staining reagents from US to India. After facing several problems from stains manufactured by local manufacturers, we thought of switching over to RA stains. The local distributor here is telling that these reagents(we are planning for procuring hematoxylin,eosin,bluing reagent and clarifier)are highly hazardous and need special packing(itself require 4000 US $). Anybody having any sort of experience about this and having idea about how RA stains can be transported/used in other parts of the world. Thanks for all your help abijag Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From NMargaryan <@t> childrensmemorial.org Thu Oct 29 12:23:02 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Oct 29 12:25:20 2009 Subject: [Histonet] AlkPhos In-Reply-To: <9b634097-bd72-4659-b8c2-c6d260e0c7e4@CMHHTCA01.childrensmemorial.org> References: <9b634097-bd72-4659-b8c2-c6d260e0c7e4@CMHHTCA01.childrensmemorial.org> Message-ID: Dear histonetters, I have to order AlkPhos kit to my IHC and, because I did not use it for the past 5-6 years, I do not know what company will best to order from. Any suggestions are appreciated, Naira From TJJ <@t> stowers.org Thu Oct 29 12:28:54 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Oct 29 12:29:12 2009 Subject: [Histonet] Surgipath Automated Slide Printer Message-ID: Is anybody in histoland using the Surgipath Automated Slide Printer, Item #04V5000? If so, could you please let me know how it's working for you? Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From scampbell <@t> celligent.net Thu Oct 29 12:31:21 2009 From: scampbell <@t> celligent.net (Campbell, Sharon) Date: Thu Oct 29 12:32:19 2009 Subject: [Histonet] new antibody validations Message-ID: Hello Histonet! We are going to be optimizing and validating new antibodies. What is the recommended number of cases for validating once we have optimized? I have heard 20 and also 40 cases. Also, How is a negative control to be optimized and validated? Thank you. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line scampbell@celligent.net From akemiat3377 <@t> yahoo.com Thu Oct 29 12:44:16 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Oct 29 12:44:50 2009 Subject: [Histonet] new antibody validations In-Reply-To: References: Message-ID: <0C7BE2C6-CF6E-4CEA-85B4-B09129BC0291@yahoo.com> Hi Sharon, This was discussed around 1 1/2 months ago in great detail. Check the histonet archives for antibody validation. Akemi Allison-Tacha BS, HT (ASCP) HTL President/Director Phoenix Lab Consulting Tele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Oct 29, 2009, at 10:31 AM, Campbell, Sharon wrote: > Hello Histonet! > We are going to be optimizing and validating new antibodies. What > is the recommended number of cases for validating once we have > optimized? I have heard 20 and also 40 cases. Also, How is a > negative control to be optimized and validated? > Thank you. > > Sharon Campbell, HTL(ASCP)CM, BSBM > Histology Supervisor > Celligent Diagnostics, LLC > 101 East W.T. Harris Blvd, Suite 1212 > Charlotte, NC 28262 > 800-524-6779 ext. 104 > 704-970-3304 Direct Line > scampbell@celligent.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruebenjcarter <@t> gmail.com Thu Oct 29 12:51:36 2009 From: ruebenjcarter <@t> gmail.com (R C) Date: Thu Oct 29 12:51:40 2009 Subject: [Histonet] Digital Pathology and storage Message-ID: <2a926e3f0910291051q34e09e64q55cfa50b9693292a@mail.gmail.com> Hi All. Regarding the recent advances in digital pathology have the standards for slide and block storage been revised for both clinical and research settings? Some believe that once a slide is digitized physical slide storage should no longer take place, while others believe storage times should be decreased. Can anyone offer information? Thank you in advance. Ruben C. From ruebenjcarter <@t> gmail.com Thu Oct 29 12:57:27 2009 From: ruebenjcarter <@t> gmail.com (R C) Date: Thu Oct 29 12:57:31 2009 Subject: [Histonet] SoCal Dako Autostainer service Message-ID: <2a926e3f0910291057w4e5f7f8by4ea4387f3bdeee8b@mail.gmail.com> Is there a listing of Dako Bioengineering groups in the San Diego area who service Autostainers? I'm having a terrible time finding (local) reliable parties to: A. Show up for appointments B. Return phone calls C. Initiate contracts These are simple business practices not extreme circumstances and our company prefers to service equipment and recycle versus purchasing every few years. Again, I'd appreciate any leads in helping me achieve a goal. Thank you. Ruben C. From michaels <@t> janelia.hhmi.org Thu Oct 29 13:18:03 2009 From: michaels <@t> janelia.hhmi.org (Michael, Susan) Date: Thu Oct 29 13:18:07 2009 Subject: [Histonet] Automatic coverslippers Message-ID: Does anyone have experience using Vectashield on an automatic coverslipper? If so, how successful was it, and what model of coverslipper were you using? What section thickness were you covering, number of sections per slide? Susan Michael, HTL From pruegg <@t> ihctech.net Thu Oct 29 13:18:26 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 29 13:19:13 2009 Subject: [Histonet] Fourth Annual International IHC Course in Boca Raton Jan 23-28, 2010 Message-ID: <6DD765789694439DB643912FC80BC986@prueggihctechlt> badze_2002@yahoo.com asked for meetings coming up in 2010. FYI Please visit http://www.pathlearning.com/Pathology_Learning_Centers/Welcome.html for detailed information of the program and how to register. This meeting is in Jan 2010 and is a very good one. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _____ From ttruscot <@t> vetmed.wsu.edu Thu Oct 29 13:35:01 2009 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Oct 29 13:35:08 2009 Subject: [Histonet] Import of staining reagents(richard allan/microm, now thermo) In-Reply-To: <336106.60837.qm@web95110.mail.in2.yahoo.com> References: <336106.60837.qm@web95110.mail.in2.yahoo.com> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4AF6A42CED5@CVMMBX.vetmed.wsu.edu> Hi Abijag, You can get MSDS forms from the company that explains any hazards associated with these reagents or any others. You can study these with your local distributor. These reagents are packed by RA to be safely shipped anywhere in this country and I would assume wouldn't need much extra to go to India. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abi jag Sent: Thursday, October 29, 2009 9:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Import of staining reagents(richard allan/microm, now thermo) Hello histonetters, This is with regard to import of Richard allan(microm, now thermo)staining reagents from US to India. After facing several problems from stains manufactured by local manufacturers, we thought of switching over to RA stains. The local distributor here is telling that these reagents(we are planning for procuring hematoxylin,eosin,bluing reagent and clarifier)are highly hazardous and need special packing(itself require 4000 US $). Anybody having any sort of experience about this and having idea about how RA stains can be transported/used in other parts of the world. Thanks for all your help abijag Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Thu Oct 29 13:45:23 2009 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Thu Oct 29 13:45:27 2009 Subject: [Histonet] RE: alternative to Glucose oxidase (GLUOX) peroxidase block In-Reply-To: <000701ca57e4$dbb82980$93287c80$@callis@bresnan.net> Message-ID: <89599.7113.qm@web113109.mail.gq1.yahoo.com> Couldn't agree with Gayle more! ? Glucose oxidase is the best protocol for blocking endogenous peroxidase but isn't entirely necessary unless you are working with hematopoietic tissue or tissue that has a large leukocyte infiltrate. For muscle standard 0.3% H202?should be fine.?Of course if you happen to be staining for leukocytes (neutrophils) in your muscle section, I would just buy the reagents and do the glucose oxidase afterall. It's simply the best. ? That being said, I do muscle IHC on frozens for endothelial markers, and use 0.3% H2O2. I do see bubbling but my sections do not come off and morphology looks great at the end. I use plus slides and dry my slides for several hours before putting at -80 deg C. This is both with PFA fixed tissue and acetone. ? Good luck, Andrea From micropathlabs <@t> yahoo.com Thu Oct 29 14:02:35 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Oct 29 14:02:40 2009 Subject: [Histonet] Digital Pathology Systems Message-ID: <403409.12988.qm@web57803.mail.re3.yahoo.com> Can anyone recommend a digital pathology system? Our pathologists are interested in viewing slides?from off-site locations. Thank you! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From Fields <@t> Northside.com Thu Oct 29 12:13:45 2009 From: Fields <@t> Northside.com (Carol Fields) Date: Thu Oct 29 15:11:05 2009 Subject: [Histonet] Her2....HELP Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D08FA7100@NSMXMS04.northside.local> Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Fields <@t> Northside.com Thu Oct 29 13:03:07 2009 From: Fields <@t> Northside.com (Carol Fields) Date: Thu Oct 29 15:11:08 2009 Subject: [Histonet] FW: Her2....HELP Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D08FA711E@NSMXMS04.northside.local> ____________________________________________ From: Carol Fields Sent: Thursday, October 29, 2009 1:14 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Her2....HELP Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Fields <@t> Northside.com Thu Oct 29 13:05:45 2009 From: Fields <@t> Northside.com (Carol Fields) Date: Thu Oct 29 15:11:10 2009 Subject: [Histonet] Her2...help Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D08FA7121@NSMXMS04.northside.local> Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From gayle.callis <@t> bresnan.net Thu Oct 29 15:12:35 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Oct 29 15:13:08 2009 Subject: [Histonet] imaging organ cultures In-Reply-To: <9FB24CE8-0ED0-4E0E-B98E-CCF5ED9672E6@mednet.ucla.edu> References: <9FB24CE8-0ED0-4E0E-B98E-CCF5ED9672E6@mednet.ucla.edu> Message-ID: <000001ca58d4$32665a00$97330e00$@callis@bresnan.net> Alice, You didn't say if your confocal was using an inverted or upright microscope setup. If inverted, there are special round glass bottom dishes with cover glass thicknesses, different diameters designed for viewing with an inverted setup. They probably work for upright microscopes too. BD Bioscience had culture plates with very small diameter wells (for inverted microscope)/ Try Mattek (just Google for website) and www.willcowells.com There were samples of WillCo Wells at the NSH Symposium/Convention, with both clear or black glass . , There are even special culture well plates for confocal use with the well diameters very small. I believe the culture wells come from BD Bioscience, and Mattek has the glass bottom petri dishes. Using a hard set antifade may help anchor your embryonic organ cultures, but these dishes also come sterile so you can do the cultures in the dish itself, followed by staining and coverslipping. Hope this helps, Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alice Fleming Sent: Wednesday, October 28, 2009 4:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] imaging organ cultures Hi, I am doing whole mount IHCs on embryonic organ cultures and imaging them on a confocal microscope. The IHCs are going well, but I'm having trouble making the tissue stay in a specific position for imaging. I have been putting the tissue in the wells of depression slides, adding about 30uL of Vectashield mounting medium, then coverslipping. This works fine, except that I have little control over the final position of the tissue-as I put the coverslip down, the tissue often moves. Any suggestions would be welcome! Is there, for example, some kind of optically neutral agarose that I could spot around the tissue before adding the Vectashield and coverslip?? Or-other ideas?? Thanks, Alice Alice Fleming Department of Human Genetics University of California Los Angeles Los Angeles, CA 90095 310-267-2456 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4552 (20091028) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4552 (20091028) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4552 (20091028) __________ The message was checked by ESET Smart Security. http://www.eset.com From Norm.Burnham <@t> propath.com Thu Oct 29 15:22:38 2009 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Thu Oct 29 15:22:43 2009 Subject: [Histonet] Transcription Benchmarks Message-ID: <82C7248978CB50469FD6BA68EBBEFE6702602654@exchange.propathlab.com> Dear Histonetters, Does someone have benchmarks they would be willing to share about the benchmarks they use for transcription? Thank you in advance. Norm Burnham From rjbuesa <@t> yahoo.com Thu Oct 29 15:37:11 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 29 15:37:16 2009 Subject: [Histonet] Transcription Benchmarks In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE6702602654@exchange.propathlab.com> Message-ID: <963187.24223.qm@web65714.mail.ac4.yahoo.com> Under separate cover I am sending something about that. Ren? J. --- On Thu, 10/29/09, Norm Burnham wrote: From: Norm Burnham Subject: [Histonet] Transcription Benchmarks To: histonet-bounces@lists.utsouthwestern.edu, Histonet@lists.utsouthwestern.edu Date: Thursday, October 29, 2009, 4:22 PM Dear Histonetters, Does someone have benchmarks they would be willing to share about the benchmarks they use for transcription? Thank you in advance. Norm Burnham _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fields <@t> Northside.com Thu Oct 29 15:54:58 2009 From: Fields <@t> Northside.com (Carol Fields) Date: Thu Oct 29 15:55:08 2009 Subject: [Histonet] CDJ Institutions Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D08FA71B1@NSMXMS04.northside.local> Also besides the Her2 policy...do any of you send CJD tissue to other institutions to process? We were sending ours out but they would not provide a report back to our Pathologists. THX again, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From trathborne <@t> somerset-healthcare.com Thu Oct 29 16:10:20 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Oct 29 16:10:26 2009 Subject: [Histonet] FW: Her2....HELP In-Reply-To: <8CEB6DA1A3F35743800669D4CFE21F7D08FA711E@NSMXMS04.northside.local> Message-ID: We let the breast fix in a bread-loafed state for the desired amount of time (usually overnight). It is then sectioned and placed in cassettes. Whatever time the tissue is in formalin on the processor should be calculated so that it doesn't exceed the 48 hour CAP/ASCO guideline. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Carol Fields Sent: Thursday, October 29, 2009 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Her2....HELP ____________________________________________ From: Carol Fields Sent: Thursday, October 29, 2009 1:14 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Her2....HELP Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From jconnelly <@t> sleh.com Thu Oct 29 16:12:03 2009 From: jconnelly <@t> sleh.com (Connelly, John) Date: Thu Oct 29 16:12:17 2009 Subject: [Histonet] EMA Message-ID: <53C8858F28EF504C9B481F0636CE404EE195A23265@SLEHEXCH01.sleh.com> I saw the earlier post on the E29 clone and the problem with meningiomas. We use the same clone and I am not too happy with it either. Does anyone have an alternative clone they are happy with. Thanks, John Connelly " Hello Histonetters, We are in the market for a nicely robust EMA that will readily stain meningiomas. We are currently using clone E29 but the formulation requires extremely long HIER and incubation times for meningiomas. We use Ventana instruments but all opinions are welcome. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596" +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From POWELL_SA <@t> mercer.edu Thu Oct 29 16:17:12 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Oct 29 16:17:21 2009 Subject: [Histonet] Her2 fixation question Message-ID: <9BF995BC0E47744E9673A41486E24EE222A539ADCF@MERCERMAIL.MercerU.local> I am sending this email for a friend who is having problems posting to histonet right now. Please reply to her at carol.fields@northside.com and not me. >From Carol Fields She writes: Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com ________________________________ From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 29 16:20:04 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Oct 29 16:20:16 2009 Subject: [Histonet] Negative controls for special stains (non-organism)?!?! Message-ID: <1AAF670737F193429070841C6B2ADD4CF74A8D56@EXMBMCB15.ucsfmedicalcenter.org> I am reposting this because I didn't get any responses and am hoping for some info from other JC-inspected labs.... Tim ***************************** Hi all, Our Joint Commission audit was just completed (first time for JC for me). We passed almost everything fine. The one thing they came up with is that we don't use "negative controls" for most of the special stains - like trichrome, congo red, etc. We use negative controls for micro-organism stains but not for the others. In fact I've never heard of anyone doing that and can't find any information in books, other lab manuals, etc. CLIA and CAP do not require negative controls for special stains. Has anyone had this issue with JC? Does anyone run "negative" controls for non-organism special stains? Frankly I'm not sure how that would be done for something like a trichrome or other purely tissue-element stains. Thanks for any insight! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA Email: tim.morken@ucsfmedctr.org From laurie.colbert <@t> huntingtonhospital.com Fri Oct 30 10:58:07 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Oct 30 10:58:11 2009 Subject: [Histonet] Returning Placentas to Patients Message-ID: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> For those of you who work at hospitals that will let patients take their placentas home, I have a question. Do you ever see these placentas - are they sent to Pathology for exam before being returned to the patient or is the placenta given directly to the patient in L&D? Laurie Colbert From piraindd <@t> upmc.edu Fri Oct 30 11:11:48 2009 From: piraindd <@t> upmc.edu (Pirain, Danielle D) Date: Fri Oct 30 11:11:54 2009 Subject: [Histonet] RE: Returning Placentas to Patients In-Reply-To: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> Message-ID: <5C4811FE75FBEC489F8E839B7E9BF51401ED8B558B@msxmbxnsprd16.acct.upmchs.net> When I worked as a Pathologist assistant at CCF we were always to receive, document, gross in path lab and then release them to the patient. Hope this helps. Danielle Pirain -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, October 30, 2009 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Returning Placentas to Patients For those of you who work at hospitals that will let patients take their placentas home, I have a question. Do you ever see these placentas - are they sent to Pathology for exam before being returned to the patient or is the placenta given directly to the patient in L&D? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Oct 30 11:24:08 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 30 11:24:13 2009 Subject: [Histonet] Negative controls for special stains (non-organism)?!?! In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF74A8D56@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CFC@LSRIEXCH1.lsmaster.lifespan.org> I don't see how negative controls for ordinary histochemical procedures would be much help unless they are the same tissue as the test sample. If you are doing a congo red for amyloid plaques in brain, I can see using a known negative brain as a control. But I don't see the point of running a brain section as a negative collagen control when you are staining a lung. What relevance would that have to the test section? From Maria.Katleba <@t> stjoe.org Fri Oct 30 11:33:43 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Oct 30 11:34:00 2009 Subject: [Histonet] RE: Returning Placentas to Patients In-Reply-To: <5C4811FE75FBEC489F8E839B7E9BF51401ED8B558B@msxmbxnsprd16.acct.upmchs.net> References: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> <5C4811FE75FBEC489F8E839B7E9BF51401ED8B558B@msxmbxnsprd16.acct.upmchs.net> Message-ID: Hi All, The problem is that when you receive it and then release it to the patient, you set your self up for a lawsuit. Have you introduced some biological hazard to it by placing it on your grossing table? How long did it sit in your counter...fridge? Some people bury it, but some people also eat it. Yes, sounds gross, but that does happen. Not sure which culture doe it...but I have heard of it. For the same reason restaurants are governed by FDA, I would imagine the potential for a lawsuits if the person who may ingest the now contaminated placenta gets sick. Just a thought. If you are going to do this, then let patient take it home. Don't even do a gross on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pirain, Danielle D Sent: Friday, October 30, 2009 9:12 AM To: 'Laurie Colbert'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Returning Placentas to Patients When I worked as a Pathologist assistant at CCF we were always to receive, document, gross in path lab and then release them to the patient. Hope this helps. Danielle Pirain -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, October 30, 2009 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Returning Placentas to Patients For those of you who work at hospitals that will let patients take their placentas home, I have a question. Do you ever see these placentas - are they sent to Pathology for exam before being returned to the patient or is the placenta given directly to the patient in L&D? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From thomas.crowell <@t> novartis.com Fri Oct 30 11:38:38 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Fri Oct 30 11:38:49 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 10/30/2009 and will not return until 11/02/2009. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From liz <@t> premierlab.com Fri Oct 30 12:07:11 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 30 12:07:42 2009 Subject: [Histonet] Digital Pathology Systems References: <403409.12988.qm@web57803.mail.re3.yahoo.com> Message-ID: Sheila We have an Aperio scanner and it works well for us. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sheila Haas Sent: Thu 10/29/2009 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital Pathology Systems Can anyone recommend a digital pathology system? Our pathologists are interested in viewing slides from off-site locations. Thank you! Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kerry.l.crabb <@t> gsk.com Fri Oct 30 12:06:37 2009 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Fri Oct 30 12:09:11 2009 Subject: [Histonet] Kerry L Crabb/PharmRD/GSK is out of the office. Message-ID: I will be out of the office starting 30-Oct-2009 and will not return until 31-Oct-2009. During my absence contact the histology lab about your anatomic path issues (483-6790) or call the APL pager at 506-0594. I will respond to messages when I return. From Rcartun <@t> harthosp.org Fri Oct 30 12:28:42 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 30 12:28:50 2009 Subject: [Histonet] Returning Placentas to Patients In-Reply-To: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> Message-ID: <4AEAEA0A.7400.0077.1@harthosp.org> We no longer will release any tissue to a patient or family member. If they want their tissue they now have to go through a funeral home. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Laurie Colbert" 10/30/2009 11:58 AM >>> For those of you who work at hospitals that will let patients take their placentas home, I have a question. Do you ever see these placentas - are they sent to Pathology for exam before being returned to the patient or is the placenta given directly to the patient in L&D? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly.Marshall <@t> ahss.org Fri Oct 30 12:55:45 2009 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Fri Oct 30 12:56:38 2009 Subject: [Histonet] patients taking home placentas Message-ID: I have come across this several times. Each time we grossed the placenta, but did not take specimens. Before they took it home I had to have them sign a release of information as well as paperwork acknowledging they understand that it is a bio-hazard. This same form is also worded to release the hospital of any blame in the event someone gets sick, or it surfaces after a big rain. Not sure if that helps but that is what my Pathologist has be doing. Good luck with this. ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From Sandra.Harrison3 <@t> va.gov Fri Oct 30 15:19:34 2009 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Oct 30 15:20:10 2009 Subject: [Histonet] Histotech opening at Minneapolis VA Message-ID: DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN. Full time Histotech. opportunity at Minneapolis VA. BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp. IHC experience a plus. Effective interpersonal skills required. Holiday, evenings and weekends off. Excellent bene's. Detail oriented. Responsible for technical and procedural operations of the dept., performing quality control, quality improvement and regulatory compliance tasks. Job will be posted on www.usajobs.gov within the next month. Please contact me if you have any questions: Sandra Harrison, Histology Supervisor, at Sandra.Harrison3@va.gov. Principals only. No recruiters please. From rjbuesa <@t> yahoo.com Fri Oct 30 15:40:32 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 30 15:40:36 2009 Subject: [Histonet] Paraform tissue holding devices: question. Message-ID: <139277.3933.qm@web65716.mail.ac4.yahoo.com> To those colleagues that are sectioning tissues held in place by these new Paraform holding devices: ? What is your experience with them? ? Have they changed your cutting productivity? ? Are you having problems with the adhesion of the sections that also include the Paraform? ? Could you please share your answers with all? Thanks! Ren? J. From collette2 <@t> mail.llnl.gov Fri Oct 30 17:40:06 2009 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Fri Oct 30 17:40:24 2009 Subject: [Histonet] TRAP stain in bone Message-ID: Hello, and Happy Friday! I am puzzled over the wide array of protocols available for TRAP staining (Tartrate-Resistant Acid Phosphatase) to stain osetoclasts in bone. I have read a few methods papers, and a few papers that use it as a method in their analysis. It seems to be more pH dependent than reagent-dependent, since I have seen reagents such as Naphthol-AS-MX phosphate and Naphthol-AS-BI phosphate being used for both acid and alkaline phosphataste stains. I also found one reference that says, "Naphthol-AS-BI-phosphate is a substrate for alkaline (1) and acidic (2, 3) phosphatase. After hydrolysis of the non-fluorescent Naphthol-AS-BI phosphate by the enzyme the resulting Naphthol-AS-BI can be measured by fluorescence methods." They recommend 0.5mM in 200mM sodium acetate, pH 5.0. Is it really that simple? Can I stain with this single reagent and photograph my sections on the fluorescent scope (given proper rinsing, mounting, etc.)? Anyone know what color it is? I got 4 filters, I'm guessing one should work. Other protocols call for Fast Violet B, or Fast Garnet GBC, I'm guessing this makes the stain either purple or red, but is there some other reason for using it for the reaction? Any recommendations or bad experiences would be much appreciated. I am able to do either paraffin or frozen sections, but prefer paraffin if possible. I already have Naphthol-AS-MX phosphate, Naphthol-AS-BI phosphate, Fast Red TR (does this work like Fast Garnet GBC, as a red stain?), and assorted acids and buffers if that makes a difference. I am happy to purchase additional reagents that will work. Thanks so much for your help and support! Sincerely, Nicole Collette Lawrence Livermore National Laboratory/ UC Berkeley From amosbrooks <@t> gmail.com Fri Oct 30 18:27:28 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Oct 30 18:27:33 2009 Subject: [Histonet] Returning Placentas to Patients Message-ID: <582736990910301627p2df9720eqb879cdee7ab285c3@mail.gmail.com> Hi, I have 2 kids 15 & 12. No pathology lab had a chance to see either placenta. I was in school when my son was born and I snagged the placenta to bring to Cobleskill because the students needed a full term placenta for cutting experience (histotechs cut placentas sometimes ... hehe). I still have the slides and blocks. My mother-in-law asked what the red bag I was lugging around was. The ensuing look of shock was the funniest thing ever. "There's something wrong with him!" she said to my wife. Her response was "You don't know the half of it." A few years later when my daughter was born, I snagged her placenta as well, since (as deranged as this may sound) I didn't want to overlook her. I have her slides & blocks as well. This time the OB/GYN wanted to look over my shoulder while I was preparing the samples. She wanted to see how we make the membrane rolls. Some folks have baby pictures from the hospital. I got that and then some! Amos Brooks Message: 24 Date: Fri, 30 Oct 2009 08:58:07 -0700 From: "Laurie Colbert" Subject: [Histonet] Returning Placentas to Patients To: Message-ID: < 57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" For those of you who work at hospitals that will let patients take their placentas home, I have a question. Do you ever see these placentas - are they sent to Pathology for exam before being returned to the patient or is the placenta given directly to the patient in L&D? Laurie Colberta From sisatwork2004 <@t> yahoo.com Sat Oct 31 19:22:53 2009 From: sisatwork2004 <@t> yahoo.com (Etta French) Date: Sat Oct 31 19:22:56 2009 Subject: [Histonet] Softpath computer program Message-ID: <422240.62425.qm@web55502.mail.re4.yahoo.com> Hi! I am interested in finding anyone out there who may be using the Softpath computer program.? If anyone is, are you using the Special Stain Worklist?? I am having an awful time getting it to work effectively for us and I don't know if the program is just not well written or if it's me.? I have asked softpath to put me in touch with someone who is using it so I can ask a few questions about the functionality of certain issues, but no luck.? I honestly don't think anyone else is trying to use it but my pathologists love it for ordering special stains and immunos.? They are not willing to go? back to ordering the old fashioned way (pen and paper). Thanks, Etta French, HT