SPAM-LOW: [Histonet] Re: B5 alternate

Patsy Ruegg pruegg <@t> ihctech.net
Sat Nov 14 13:32:07 CST 2009


Good luck with that idea of sitting in formalin overnight, most of the
Pathologist I have worked with over the last 20 years seem to be getting
more resistant rather than more tolerant to that idea, to my chagrin. 

Cheers,
Patsy

Btw
I was saddened to hear of Russ Allison's passing.  I did consider him a good
friend and knowledgeable histopathology colleague, one of the great ones
left us in his passing.

Regards,
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Friday, November 13, 2009 2:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Re: B5 alternate

I loved B5 fixative, and before that Zenker's, but they're gone and
they're not coming back.

In my personal expereience in a number of pathology services, the
various muchly-touted B5 substitutes offer very little morphologic
advantage over neutral buffered formalin.

Unless it's fixed pretty early in the morning, tissue needs to sit
overnight in neutral buffered formalin before being decalcified or
processed. Pathologists and their clinicians are eventually going to
have to learn to live with this delay.

Bob Richmond
Samurai Pathologist
Knoxville TN

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