From birnbaumm <@t> asaf.health.gov.il Sun Nov 1 03:49:39 2009 From: birnbaumm <@t> asaf.health.gov.il (birnbaumm@asaf.health.gov.il) Date: Sun Nov 1 03:50:31 2009 Subject: [Histonet] FISH on B.M. Message-ID: <054452CCC076BE4DA21E46AE95E32EC3277B4C@mail2.asaf.health.gov.il> Helo all, We do FISH assay on FFPE tissue with "spot light tissue pretreatment" of invitrogen. It do works on most of the tissues, but not on Bone Marrow tissue. Do you have any idea? Thanks Dr. Miriam Birnbaum Pathology Asaf Hrofeh Medical Center Israel From gu.lang <@t> gmx.at Sun Nov 1 06:50:29 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Nov 1 06:50:36 2009 Subject: AW: [Histonet] FISH on B.M. In-Reply-To: <054452CCC076BE4DA21E46AE95E32EC3277B4C@mail2.asaf.health.gov.il> References: <054452CCC076BE4DA21E46AE95E32EC3277B4C@mail2.asaf.health.gov.il> Message-ID: <97DED1A3A4D94310B6C2829FBCCF6671@dielangs.at> Miriam, do you do FISH on bone marrow trephine biopsies, that have to be decalcified before cutting and staining? If decalcifing is performed with acid like formic acid or even hydrochloric acid, DNA is degraded to smaller fragments. Successfull hybridization of this fragments is kind of chance, because it could easily happen that the wanted DNA-sequence is destroyed. If the DNA-sequence is destroyed, there is no way to restore it. Decalcifing should be performed with EDTA for a good preservation of DNA. I have performed CISH on formic acid - decalcified bone marrow trephines with success. But we demonstrated mRNA of light chains, that are usually found in a big amount in the cytoplasm. So degrading a part of the mRNA would not influence the final result. Our protocol for BMTB is one day fixation in NBF, next day decal with 5-10% formic acid with 5-10% formaldehyde for 8 hours, then processing like the other routine specimens. Hope this helps Gudrun Lang Histolab, AKH Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von birnbaumm@asaf.health.gov.il Gesendet: Sonntag, 01. November 2009 10:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH on B.M. Helo all, We do FISH assay on FFPE tissue with "spot light tissue pretreatment" of invitrogen. It do works on most of the tissues, but not on Bone Marrow tissue. Do you have any idea? Thanks Dr. Miriam Birnbaum Pathology Asaf Hrofeh Medical Center Israel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Sun Nov 1 15:50:58 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Sun Nov 1 15:51:09 2009 Subject: [Histonet] VA Beach mini-mtg. Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A775C@wlmmsx01.nemours.org> Histonetter's in the VA, MD and DC area...reminder of the fall mini-LEARN and EARN to be held this Saturday, November 7th 2009, at the Virginia Beach Higher Education Center. Six (6) CEU's available, Two Speakers: Carol Barone HT(ASCP) BA - Frozen Sections: Materials , Methods and Mastery (3 CEU's) and Osman Ouattara - CT(ASCP) BS - Cytology. One Hour Open Forum will follow workshops. Open to all. Call 302-651-6827 for information on registration... Discounted registration to NSH and Region II State members. Registration can be mailed, or will be accepted at the door (checks only, however). Lunch is included with full day registration. For more information:cbarone@nemours.org. The most in-expensive way to earn your needed CEU's. From lpwenk <@t> sbcglobal.net Mon Nov 2 04:07:21 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 2 04:07:28 2009 Subject: [Histonet] Schiff mystery Message-ID: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> Need the help of the Histonet chemistry gurus. This has happened the last 2 times the students and I made up Schiff. Two different set of students. We make up the Schiff, and use it the next day, and it works correctly (stains the glycogen in liver and neutral mucins in intestine a bright magenta). Store in refrig. 1 week later, when we go to stain fungus with PAS, the Schiff doesn't work. Try the same liver and small intestine controls. Doesn't work at all. No magenta, no pink, nothing on the slides. When going into the water rinse after Schiff, the water turns pink with the newly made Schiff, but 1 week later, there is no color change in the water rinse. When we make up the Schiff, we show the students the Schiff quality assessment, where we put a few drops of formaldehyde in it, and the Schiff changes to purple immediately. Works great newly made. One week later, when we can't get the Schiff to stain, there is no color change with formaldehyde. None. It is the same procedure we've been using for years to make up the Schiff. It is the same procedure our histology lab uses. Their Shiff lasts for months. Ours last for a few days. I think it might be one of our reagents is going bad. Histology lab has their own reagents, the School has our own. - The basic fuchsin is OK, as the Kinyoun works great. - The hydrochloric acid was taken from histology, so I'm guessing it's OK. - The charcoal is fairly new. Could that be the problem? Why? - We are using sodium metabisulfite, not sodium sulfite or sulfate. At least that's what the bottle says. Does this break down and go bad? Need all the help we can get. This is spooky, Halloween or no Halloween. Peggy Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 From mahaynes <@t> cnmc.org Mon Nov 2 07:06:38 2009 From: mahaynes <@t> cnmc.org (Haynes, MaryAnne) Date: Mon Nov 2 07:06:43 2009 Subject: [Histonet] Schiff mystery In-Reply-To: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> References: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> Message-ID: I experienced a similar problem about twenty years ago. We determined it was residual soap on the glassware. Glassware washer was not rinsing properly. Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, November 02, 2009 05:07 To: histonet@lists.utsouthwestern.edu Cc: 'Peggy Wenk' Subject: [Histonet] Schiff mystery Need the help of the Histonet chemistry gurus. This has happened the last 2 times the students and I made up Schiff. Two different set of students. We make up the Schiff, and use it the next day, and it works correctly (stains the glycogen in liver and neutral mucins in intestine a bright magenta). Store in refrig. 1 week later, when we go to stain fungus with PAS, the Schiff doesn't work. Try the same liver and small intestine controls. Doesn't work at all. No magenta, no pink, nothing on the slides. When going into the water rinse after Schiff, the water turns pink with the newly made Schiff, but 1 week later, there is no color change in the water rinse. When we make up the Schiff, we show the students the Schiff quality assessment, where we put a few drops of formaldehyde in it, and the Schiff changes to purple immediately. Works great newly made. One week later, when we can't get the Schiff to stain, there is no color change with formaldehyde. None. It is the same procedure we've been using for years to make up the Schiff. It is the same procedure our histology lab uses. Their Shiff lasts for months. Ours last for a few days. I think it might be one of our reagents is going bad. Histology lab has their own reagents, the School has our own. - The basic fuchsin is OK, as the Kinyoun works great. - The hydrochloric acid was taken from histology, so I'm guessing it's OK. - The charcoal is fairly new. Could that be the problem? Why? - We are using sodium metabisulfite, not sodium sulfite or sulfate. At least that's what the bottle says. Does this break down and go bad? Need all the help we can get. This is spooky, Halloween or no Halloween. Peggy Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From cmiller <@t> physlab.com Mon Nov 2 09:06:40 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Nov 2 09:06:49 2009 Subject: [Histonet] Returning Placentas to Patients In-Reply-To: <4AEAEA0A.7400.0077.1@harthosp.org> References: <57BE698966D5C54EAE8612E8941D7683070BA418@EXCHANGE3.huntingtonhospital.com> <4AEAEA0A.7400.0077.1@harthosp.org> Message-ID: Smart!! Cheryl Miller HT ASCP Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 30, 2009 12:29 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Returning Placentas to Patients We no longer will release any tissue to a patient or family member. If they want their tissue they now have to go through a funeral home. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Laurie Colbert" 10/30/2009 11:58 AM >>> For those of you who work at hospitals that will let patients take their placentas home, I have a question. Do you ever see these placentas - are they sent to Pathology for exam before being returned to the patient or is the placenta given directly to the patient in L&D? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From silvinamolinuevo <@t> yahoo.com.ar Mon Nov 2 10:23:44 2009 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Mon Nov 2 10:23:47 2009 Subject: [Histonet] Re: TRAP stain in bone (Nicole Collette) Message-ID: <322270.49947.qm@web31605.mail.mud.yahoo.com> Hello Nicole!! In our laboratory we use a kit from Sigma to stain osteoclasts in bone. It uses Fast Garnet and works very well with paraffin sections. I think that using Naphthol-AS-MX phosphate, Naphthol-AS-BI phosphate it would be suitable. But the important thing is that you have to use a Tartrate buffer to enhace especificity (osteoclastic phosphatases are tartrete resistant). If you need additional help please feel free to contact me. Best regards, Silvina ------------------------------ Message: 7 Date: Fri, 30 Oct 2009 15:40:06 -0700 From: Nicole Collette Subject: [Histonet] TRAP stain in bone To: Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Hello, and Happy Friday! I am puzzled over the wide array of protocols available for TRAP staining (Tartrate-Resistant Acid Phosphatase) to stain osetoclasts in bone. I have read a few methods papers, and a few papers that use it as a method in their analysis. It seems to be more pH dependent than reagent-dependent, since I have seen reagents such as Naphthol-AS-MX phosphate and Naphthol-AS-BI phosphate being used for both acid and alkaline phosphataste stains. I also found one reference that says, "Naphthol-AS-BI-phosphate is a substrate for alkaline (1) and acidic (2, 3) phosphatase. After hydrolysis of the non-fluorescent Naphthol-AS-BI phosphate by the enzyme the resulting Naphthol-AS-BI can be measured by fluorescence methods." They recommend 0.5mM in 200mM sodium acetate, pH 5.0. Is it really that simple? Can I stain with this single reagent and photograph my sections on the fluorescent scope (given proper rinsing, mounting, etc.)? Anyone know what color it is? I got 4 filters, I'm guessing one should work. Other protocols call for Fast Violet B, or Fast Garnet GBC, I'm guessing this makes the stain either purple or red, but is there some other reason for using it for the reaction? Any recommendations or bad experiences would be much appreciated. I am able to do either paraffin or frozen sections, but prefer paraffin if possible. I already have Naphthol-AS-MX phosphate, Naphthol-AS-BI phosphate, Fast Red TR (does this work like Fast Garnet GBC, as a red stain?), and assorted acids and buffers if that makes a difference. I am happy to purchase additional reagents that will work. Thanks so much for your help and support! Sincerely, Nicole Collette Lawrence Livermore National Laboratory/ UC Berkeley Yahoo! Cocina Encontra las mejores recetas con Yahoo! Cocina. http://ar.mujer.yahoo.com/cocina/ From talulahgosh <@t> gmail.com Mon Nov 2 11:40:09 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Nov 2 11:40:13 2009 Subject: [Histonet] Schiff mystery In-Reply-To: References: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> Message-ID: Oh man, worst problem ever. I hand wash our dishes and rinse them 12 times so we don't EVER have that problem. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum On Mon, Nov 2, 2009 at 8:06 AM, Haynes, MaryAnne wrote: > I experienced a similar problem about twenty years ago. We determined > it was residual soap on the glassware. Glassware washer was not rinsing > properly. > > > Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) > Pathology Manager > Children's National Medical Center > Department of Anatomic Pathology > 111 Michigan Ave NW > Washington, DC 20010 > 202-476-4311 (office) > 202 476-4030 (fax) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & > Peggy Wenk > Sent: Monday, November 02, 2009 05:07 > To: histonet@lists.utsouthwestern.edu > Cc: 'Peggy Wenk' > Subject: [Histonet] Schiff mystery > > Need the help of the Histonet chemistry gurus. > > This has happened the last 2 times the students and I made up Schiff. > Two > different set of students. > > We make up the Schiff, and use it the next day, and it works correctly > (stains the glycogen in liver and neutral mucins in intestine a bright > magenta). Store in refrig. 1 week later, when we go to stain fungus with > PAS, the Schiff doesn't work. Try the same liver and small intestine > controls. Doesn't work at all. No magenta, no pink, nothing on the > slides. > > When going into the water rinse after Schiff, the water turns pink with > the > newly made Schiff, but 1 week later, there is no color change in the > water > rinse. > > When we make up the Schiff, we show the students the Schiff quality > assessment, where we put a few drops of formaldehyde in it, and the > Schiff > changes to purple immediately. Works great newly made. One week later, > when > we can't get the Schiff to stain, there is no color change with > formaldehyde. None. > > It is the same procedure we've been using for years to make up the > Schiff. > It is the same procedure our histology lab uses. Their Shiff lasts for > months. Ours last for a few days. > > I think it might be one of our reagents is going bad. Histology lab has > their own reagents, the School has our own. > - The basic fuchsin is OK, as the Kinyoun works great. > - The hydrochloric acid was taken from histology, so I'm guessing it's > OK. > - The charcoal is fairly new. Could that be the problem? Why? > - We are using sodium metabisulfite, not sodium sulfite or sulfate. At > least > that's what the bottle says. Does this break down and go bad? > > Need all the help we can get. This is spooky, Halloween or no Halloween. > > Peggy Wenk, HTL(ASCP)SLS > Schools of Histotechnology > Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended > recipient(s) and may contain confidential and privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mweirauch <@t> crittenton.com Mon Nov 2 11:44:29 2009 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Mon Nov 2 11:45:05 2009 Subject: [Histonet] HER2 on upper GI biopsies Message-ID: <5DA184CE5B6A624D8370924ED8835736046C8172@CHMC-MAIL03.crittenton.net> We have had some oncologists asking for HER2 by IHC then reflexing for FISH if the IHC is negative or equivocal on upper GI tumors. Our pathologists have been lookin for a recommended guideline to reflex for negatives in these types of cases, but haven't found any -- is anyone aware of FISH testing guidelines on GI cases? Thanks in advance! From marktarango <@t> gmail.com Mon Nov 2 11:48:03 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Nov 2 11:48:06 2009 Subject: [Histonet] HER2 on upper GI biopsies In-Reply-To: <5DA184CE5B6A624D8370924ED8835736046C8172@CHMC-MAIL03.crittenton.net> References: <5DA184CE5B6A624D8370924ED8835736046C8172@CHMC-MAIL03.crittenton.net> Message-ID: <5b6eb13e0911020948l5cd8d9edh9f61aa941a915ae5@mail.gmail.com> We've been getting these requests lately too. I've been wondering if we need to monitor fixation time for these specimens, since Her2neu is a possibility. Mark Tarango On Mon, Nov 2, 2009 at 9:44 AM, Maray Weirauch wrote: > We have had some oncologists asking for HER2 by IHC then reflexing for FISH > if the IHC is negative or equivocal on upper GI tumors. > Our pathologists have been lookin for a recommended guideline to reflex for > negatives in these types of cases, but haven't found any -- is anyone aware > of FISH testing guidelines on GI cases? > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terri.Bishop <@t> SPIndustries.com Mon Nov 2 12:54:48 2009 From: Terri.Bishop <@t> SPIndustries.com (Terri Bishop) Date: Mon Nov 2 12:55:36 2009 Subject: [Histonet] Multi Cool Bath replacement for Histobath Message-ID: <74F3A1E9EA68A64AB5C7A21C564AD46A024B6F4208@Mail.ad.spindustries.com> I wanted to take this opportunity to introduce the FTS Brand Multi Cool Bath as a replacement for the Histobath. The Multi Cool comes with a 4 liter or 8 liter stainless steel reservoir. It is offered with temperature control if desired with a temperature range of -80C to +100C. Please view the data sheet on our website at http://www.ftssystems.com/multicoolbath.htm. If you have any questions or if you need additional information, please feel free to contact me at 845-687-5359. Regards, Terri Bishop Sr. Technical Sales Associate - FTS Thermal SP Industries 3538 Main Street P O Box 158 Stone Ridge, NY 12484-0158 Tel: 845-687-5359 Fax: 845-687-7481 E: terri.bishop@spindustries.com www.ftssystems.com From cbarone <@t> NEMOURS.ORG Mon Nov 2 13:29:54 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Nov 2 13:30:11 2009 Subject: [Histonet] The most in-expensive way to earn CEU's.... Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7762@wlmmsx01.nemours.org> The most in-expensive way to earn your needed CEU's....didn't make it to Birmingham? Come to Virginia Beach, Virginia!!!! This week-end!!!!! A day to learn and earn CEU's, for as little as $35.00. Open registration at the door.....Virgina and surounding states ...reminder of the fall mini-LEARN and EARN to be held this Saturday, November 7th 2009, at the Virginia Beach Higher Education Center. Six (6) CEU's available, Two Topics: Frozen Sections: Materials , Methods and Mastery (3 CEU's) and Cytology. One Hour Open Forum will follow workshops. Open to all. Call 302-651-6827 for information on registration...or contact Thompson, Sophie K. [sxthomps@odu.edu], ODU Discounted registration to NSH and Region II State members. Registration will be accepted at the door (checks only, however). Lunch is included with full day registration. For more information:cbarone@nemours.org. The Histonet is about continuing education and networking....and so are we!!!! Come on down! From jackdodo <@t> msn.com Mon Nov 2 13:56:20 2009 From: jackdodo <@t> msn.com (Jack Dodo) Date: Mon Nov 2 13:56:26 2009 Subject: [Histonet] CAP IHC Negative control Regs Message-ID: I am current with what is needed. I need to find past regulations (CAP) from 2007 and 2006. I can't seem to find them no matter how I search. Can anyone help? I am trying to prove a point to my Director. Thanks in advance. _________________________________________________________________ New Windows 7: Find the right PC for you. http://www.microsoft.com/windows/pc-scout/default.aspx?CBID=wl&ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_pcscout:112009 From mcauliff <@t> umdnj.edu Mon Nov 2 14:55:43 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Nov 2 14:53:52 2009 Subject: [Histonet] Schiff mystery In-Reply-To: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> References: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> Message-ID: <4AEF474F.4080809@umdnj.edu> There are several factors that influence the potency of Schiff's reagent. The quality of the basic fushsin. How much activated charcoal is used to decolorize the reagent. It is important to use the minimum amount that gives a clear (or almost clear) solution. For the test, add a drop or two of Shiffs to formaldehyde, not the other way around. And, as someone else mentioned, properly cleaned glassware. Geoff Lee & Peggy Wenk wrote: > Need the help of the Histonet chemistry gurus. > > This has happened the last 2 times the students and I made up Schiff. Two > different set of students. > > We make up the Schiff, and use it the next day, and it works correctly > (stains the glycogen in liver and neutral mucins in intestine a bright > magenta). Store in refrig. 1 week later, when we go to stain fungus with > PAS, the Schiff doesn't work. Try the same liver and small intestine > controls. Doesn't work at all. No magenta, no pink, nothing on the slides. > > When going into the water rinse after Schiff, the water turns pink with the > newly made Schiff, but 1 week later, there is no color change in the water > rinse. > > When we make up the Schiff, we show the students the Schiff quality > assessment, where we put a few drops of formaldehyde in it, and the Schiff > changes to purple immediately. Works great newly made. One week later, when > we can't get the Schiff to stain, there is no color change with > formaldehyde. None. > > It is the same procedure we've been using for years to make up the Schiff. > It is the same procedure our histology lab uses. Their Shiff lasts for > months. Ours last for a few days. > > I think it might be one of our reagents is going bad. Histology lab has > their own reagents, the School has our own. > - The basic fuchsin is OK, as the Kinyoun works great. > - The hydrochloric acid was taken from histology, so I'm guessing it's OK. > - The charcoal is fairly new. Could that be the problem? Why? > - We are using sodium metabisulfite, not sodium sulfite or sulfate. At least > that's what the bottle says. Does this break down and go bad? > > Need all the help we can get. This is spooky, Halloween or no Halloween. > > Peggy Wenk, HTL(ASCP)SLS > Schools of Histotechnology > Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From contact <@t> excaliburpathology.com Mon Nov 2 15:15:29 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Nov 2 15:15:34 2009 Subject: [Histonet] Schiff mystery In-Reply-To: <4AEF474F.4080809@umdnj.edu> References: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> <4AEF474F.4080809@umdnj.edu> Message-ID: <461094.76443.qm@web1114.biz.mail.sk1.yahoo.com> I have always made my Schiff's by the AFIP McManus Method for Glycogen. Dissolve 1.0 gm basic fuchsin in 200 ml hot distilled water. Bring to boiling point. Cool to 50C. Filter and add 20 ml N HCl. Cool further and add 1.0 gm anhydrous sodium bisulfite or sodium metabisulfite. Keep in the dark for 48 hours until solution becomes straw colored. Store in refrigerator. I usually make 500ml total, no charcoal needed, it lasts for 2+ years, and has never failed me. Paula ________________________________ From: Geoff McAuliffe To: lpwenk@sbcglobal.net Cc: histonet@lists.utsouthwestern.edu; Peggy Wenk Sent: Mon, November 2, 2009 2:55:43 PM Subject: Re: [Histonet] Schiff mystery There are several factors that influence the potency of Schiff's reagent. The quality of the basic fushsin. How much activated charcoal is used to decolorize the reagent. It is important to use the minimum amount that gives a clear (or almost clear) solution. For the test, add a drop or two of Shiffs to formaldehyde, not the other way around. And, as someone else mentioned, properly cleaned glassware. Geoff Lee & Peggy Wenk wrote: > Need the help of the Histonet chemistry gurus. > > This has happened the last 2 times the students and I made up Schiff. Two > different set of students. > > We make up the Schiff, and use it the next day, and it works correctly > (stains the glycogen in liver and neutral mucins in intestine a bright > magenta). Store in refrig. 1 week later, when we go to stain fungus with > PAS, the Schiff doesn't work. Try the same liver and small intestine > controls. Doesn't work at all. No magenta, no pink, nothing on the slides. > > When going into the water rinse after Schiff, the water turns pink with the > newly made Schiff, but 1 week later, there is no color change in the water > rinse. > > When we make up the Schiff, we show the students the Schiff quality > assessment, where we put a few drops of formaldehyde in it, and the Schiff > changes to purple immediately. Works great newly made. One week later, when > we can't get the Schiff to stain, there is no color change with > formaldehyde. None. > > It is the same procedure we've been using for years to make up the Schiff. > It is the same procedure our histology lab uses. Their Shiff lasts for > months. Ours last for a few days. > > I think it might be one of our reagents is going bad. Histology lab has > their own reagents, the School has our own. > - The basic fuchsin is OK, as the Kinyoun works great. > - The hydrochloric acid was taken from histology, so I'm guessing it's OK. > - The charcoal is fairly new. Could that be the problem? Why? > - We are using sodium metabisulfite, not sodium sulfite or sulfate. At least > that's what the bottle says. Does this break down and go bad? > > Need all the help we can get. This is spooky, Halloween or no Halloween. > > Peggy Wenk, HTL(ASCP)SLS > Schools of Histotechnology > Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Mon Nov 2 15:31:05 2009 From: alisha <@t> ka-recruiting.com (Alisha Taylor) Date: Mon Nov 2 15:30:59 2009 Subject: [Histonet] Job Opportunities for Histotechnologists! Message-ID: <172627417.1257197465656.JavaMail.cfservice@webserver54> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below are some Histotechnologist positions we are currently working on: Histology Supervisor - Georgia (1st shift) I am currently working with an over 500 bed acute care teaching hospital in GA. My client is looking for someone who is HTL(ASCP) certified, has at least 5 years of Histology experience and at least 3 years of supervisory/lead/management experience. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histotechnologist- Georgia (1st shift) I am currently working with an over 500 bed acute care teaching hospital in GA. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified and has at least 1+ year of Histology experience. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histotechnologist - Southern CA (all shifts) I am currently working with one of the largest reference labs in the country. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified and has 1+ years experience in histology. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histology Supervisor- Southern CA (3rd shift) I am currently working with one of the largest reference labs in the country. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified, has at least 5 years of Histology experience, and has at least 3 years in a leadership role. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Pathology Manager - Bay Area, CA (1st shift) I am currently working with a well-known Bay Area hospital system. My client is looking for someone to oversee two hospital histology labs and their one independant cytology lab. My client is looking for somone who is HTL(ASCP) certified, has at least 5 years of Histology experience and at least 3 years of supervisory/lead/management experience OR someone who is CLS(ASCP) certified, with a CA license, and who has experience as a Pathology Manager. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histotechnologist - Boston, MA (1st shift) I am currently working with a Boston area teaching hospital. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified and has 3+ years experience in histology. My client provides an excellent compensation package including a competitive salary and full benefits. Histotechnologist - Oklahoma I am currently working with a well-known hospital in Oklahoma. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified and has 1+ years experience in histology. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histology Supervisor- Oklahoma (1st shift) I am currently working with a well-known hospital in southern Oklahoma. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified, has at least 5 years of Histology experience, and has at least 3 years in a leadership role. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histotechnologist - New York City, NY (3rd shift) I am currently working with one of the top teaching hospitals in the country and the top hospital system in NY State. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified and has 1+ years experience in histology, and has a NY State Histology license. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Pathology Manager - New York City, NY (1st shift) I am currently working with one of the top teaching hospitals in the country and the top hospital system in NY State. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified, has a NY STate histology license, has 5+ years experience in histology, and has 3+ years in a leadership role. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Histotechnologist - Otsego County, NY (Upstate NY) I am currently working with a magnet teaching hospital in Upstate NY. My client is looking for someone who is HT(ASCP) or HTL(ASCP) certified and has 1+ years experience in histology, and has a NY State Histology license. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Laboratory Opportunities: Cytotech (Bench and Management) * Long Island, NY - Cytotech * New York City, NY - Cytotech Lab Supervisor/Management Opportunities: * GA - Atlanta - Evening Lab Supervisor * PA - Pittsburg - 1st shift Supervisor * NM - Lab Manager * NJ - Lab Supervisor (BB experience preferred) * CA- Southern - Autochemistry Lab Supervisor * NY - NY City -Hematology/Chemisty Lab Supervisor (1st or 2nd shift) * WA - Clinical Immunogenetics Lab Manager * NY - NY City - Lead Supervisor Hematology/Chemistry 2nd shift * NV - Las Vegas - Flow Cytometry Supervisor * NH - Manager of Lab Technical Operations Blood Bank (Bench and Management) * Southern California - Blood Bank Specialist 1st shift * CA - San Francisco - Blood Bank Technologist 1st shift * CA - Bay Area - Transfusion Services Manager * Anywhere in Country - Blood Bank Trainer (must have experience with LIS and Cerner Milenium) * Anywhere in Country - Blood Bank Analyst (must have experience with LIS and Cerner Milenium) * VA - Blood Bank Supervisor - 2nd shift * New York City - Blood Bank Technologist 3rd shift * TN - Blood Bank Supervisor 2nd shift * NY - Otsego County - Blood Bank Technologist 1st shift * WA - Seattle - Transfusion Technologist 3rd shift * CT- Blood Bank Specialist/ Supervisor * CT - MT(ASCP) POCT Coordinator Microbiology (Bench and Management) * New York City - Microbiology Technologist (1st or 3rd shift) * New York City - Microbiology/Mycology (1st shift) * New York City - Microbiology Supervisor (1st shift) * Southern CA - Microbiology Technologist (all shifts) * Upstate NY - Microbiology Supervisor (1st shift) Medical Technologist/ Clinical Lab Scientist: * Southern CA - Clinical Lab Scientist (all shifts) * Southern CA - Hematology Technologist 3rd shift * Southern CA - Immunology Technologist 1st shift * Southern CA - Serology Technologist - 1st shift * Southern CA - Toxicology Technologist - 1st shift * Treasure Coast, FL - Medical Technologist (3rd shift) * West Virginia - Medical Technologist (3rd shift - Three 12 hours shifts) * New York City - Hematology Technologist (2nd and 3rd shift) * New York City - Bone Marrow Technologist (1st shift) * New York City - Hematology/Chemistry Lead Technologist (1st and 2nd shift) * New York City - Chemistry Medical Technologist (all shifts) * Westchester County, NY - Medical Technologist (1st shift) * Upstate NY - Medical Technologist (2nd shift) * Upstate NY - Part Time Medical Technologist * TX - Houston - Medical Technologist (3rd shift, 7on 7off) * Otsego County, NY - Medical Technologist (all shifts) * Otsego County, NY - Medical Technologist Specialist (1st shift, needs Quality Control experience) * Phoenix, AZ - Medical Technologist 3rd shift * South of Boston, MA - Lead Medical Technologist * CT - Medical Technologist 3rd Shift * TN- Medical Technologist (BB) - 1st shift * TN - MLT (BB) - 2nd shift Cytogenetics: * CA - Southern - Cytogenetic Technologist * New York City - Cytogenetic Technologist LIS Positions (must have experience): * Anywhere in Country - Blood Bank Trainer (must have experience with LIS and Cerner Milenium) * Anywhere in Country - Blood Bank Analyst (must have experience with LIS and Cerner Milenium) * WV - LIS Analyst * New York City - LIS Analyst * OK - LIS Manager If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha R. Taylor, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com * Please note change in address and phone number From eroy <@t> uiuc.edu Mon Nov 2 20:24:03 2009 From: eroy <@t> uiuc.edu (Edward Roy) Date: Mon Nov 2 20:24:21 2009 Subject: [Histonet] Compresstome Message-ID: <9416F249-A252-4B17-A9CD-CBCA907F4DFA@uiuc.edu> At the Society for Neuroscience meeting I saw a variation on vibratomes called a Compresstome, by Precisionary Instruments. Has anyone tried this instrument? They claim they can do 10 micron sections of formaldehyde fixed mouse brain and do free-floating IHC with them. Thanks Ed Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 From mtighe <@t> trudeauinstitute.org Tue Nov 3 07:55:30 2009 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Nov 3 07:55:39 2009 Subject: [Histonet] Viral RNA probes Message-ID: <4AEFF00A.26E4.00EE.0@trudeauinstitute.org> Does anyone know of a company(s) that sells/makes ISH probes to viral RNA (PR8 and or Sendai) or a company that will make custom probes. Thanks for any help!! Mike From Fields <@t> Northside.com Tue Nov 3 08:22:47 2009 From: Fields <@t> Northside.com (Carol Fields) Date: Tue Nov 3 08:22:57 2009 Subject: [Histonet] Her2 fixation question In-Reply-To: <9BF995BC0E47744E9673A41486E24EE222A539ADCF@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE222A539ADCF@MERCERMAIL.MercerU.local> Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D08FA765F@NSMXMS04.northside.local> Thank you all for help with the Her 2 policy. I was really stuck writing the processing piece. I was able to finish the policy ....now to get our docs to pass it. THX again for the great help! Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, October 29, 2009 5:17 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Her2 fixation question I am sending this email for a friend who is having problems posting to histonet right now. Please reply to her at carol.fields@northside.com and not me. >From Carol Fields She writes: Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From rjbuesa <@t> yahoo.com Tue Nov 3 11:08:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 3 11:08:53 2009 Subject: [Histonet] Cutting Paraform tissue holding devices: question. Message-ID: <728783.55985.qm@web65713.mail.ac4.yahoo.com> Questions to those colleagues that are using the Paraform tissue holding devices: ? 1- Have you had any problems cutting these holding devices? ? 2- Has your cutting productivity been reduces, at least initially? ? 3- Have you had problems with the adhesion of the sections that also include the Paraform? ? Could you please share your answers with all? ? Thanks! Ren? J. From vapatpxs <@t> yahoo.com Tue Nov 3 11:32:53 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Tue Nov 3 11:32:56 2009 Subject: [Histonet] BSL-2 question Message-ID: <136226.61942.qm@web46114.mail.sp1.yahoo.com> Hello Everybody out there in virus land, I am getting a room ready for BSL-2 level virus imaging. I can't get any information regarding how safe the cells are once the virus has been transfected (?) in to them. I have a BSL-2 level bio-hood, I have a room with a door and I have a brand, spanking new deconvolution with FRET and an incubator for live cell imaging. I need to know if the people in the room are safe from the virus once it's inside the cells. I can't get the air pressure changed in the room so I need to know what other precautions we might need to take. Thanks to all who can give me any pertinent information. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. From Michael.Owen <@t> fda.hhs.gov Tue Nov 3 11:56:45 2009 From: Michael.Owen <@t> fda.hhs.gov (Owen, Michael P) Date: Tue Nov 3 11:56:57 2009 Subject: [Histonet] BSL-2 question In-Reply-To: <136226.61942.qm@web46114.mail.sp1.yahoo.com> References: <136226.61942.qm@web46114.mail.sp1.yahoo.com> Message-ID: <6B1915839B67AD46B88E6BE8F27376BB19FB5C@FMD3VS031.fda.gov> I posted the question to the ABSA Biosafety Discussion Forum. I will post replies I receive on the Histonet List. American Biological Safety Association (ABSA) http://www.absa.org ABSA - Resources http://www.absa.org/resmenu.html ABSA - Resources - E-mail Groups http://www.absa.org/resgroups.html Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From MadaryJ <@t> MedImmune.com Tue Nov 3 12:30:04 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Nov 3 12:31:51 2009 Subject: [Histonet] Peggy Wenk Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13A53916@MD1EV002.medimmune.com> I wanted to say to everyone that among all of the top folks we have out there including Peggy it is nice to know that Peggy actually uses the Histonet to GET info not only give out such great advice. In answering the question the only thing that screams out at me is technique of the students. I mean I only taught for a year at AFIP back in the 80's but over the years have had scores of interns, students, techs. I mean are they doing something before to break down the schiffs like placing in some seriously alkaline solution, water have minerals going back into the schiffs? Great question but my note is meant to compliment Peggy. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From rsrichmond <@t> gmail.com Tue Nov 3 12:57:47 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Nov 3 12:57:52 2009 Subject: [Histonet] Re: Multi Cool Bath replacement for Histobath Message-ID: Terri Bishop at SP Industries in Stone Ridge NY tells us: >>I wanted to take this opportunity to introduce the FTS Brand Multi Cool Bath as a replacement for the Histobath. - Please view the data sheet on our website at http://www.ftssystems.com/multicoolbath.htm << The Web site doesn't clearly indicate the potential usefulness of this product in surgical pathology - for preparing specimens for frozen section. I think a lot of pathologists, Mohs surgeons, and other users of frozen section technology will be interested in the Multi Cool Bath. Can you give us any idea what it will cost? The Web site doesn't tell you what the cooling liquid is. As I've expressed a number of times (see the HistoNet archives), I thought a major problem with the Histobath was its use of acetone or 2-methylbutane (isopentane), both serious fire and explosion hazards in a pathology laboratory. Can the Multi Cool Bath be filled with a non-flammable coolant such as 3M's Novec Engineered Fluid HFE-7100,a "segregated hydrofluoroether (HFE)", methyl nonafluoroisobutyl ether, C4F9-O-CH3? Is this fluid compatible with the Multi Cool Bath? Note a competing product, Alan Bright's (Bright Instrument Co., in the UK) Clini-RF Rapid Freezer. I have no commercial connection with any of these companies. Bob Richmond Samurai Pathologist Knoxville TN From JWeems <@t> sjha.org Tue Nov 3 12:58:49 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Nov 3 12:58:51 2009 Subject: [Histonet] Arm Rests Message-ID: Does anyone have a recommendation for microtome arm rests? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cls71877 <@t> sbcglobal.net Tue Nov 3 13:00:32 2009 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Tue Nov 3 13:00:35 2009 Subject: [Histonet] Diff Quik CPT code Message-ID: <699556.9372.qm@web81206.mail.mud.yahoo.com> Hello, I was wondering what CPT code is used for performing a Diff Quik to demonstrate H. pylori?? Can anyone help? Thanks, Cristi From TJJ <@t> stowers.org Tue Nov 3 13:01:07 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Nov 3 13:01:15 2009 Subject: [Histonet] Re: Compresstome Message-ID: Ed, We have a 2 year old model of the Precisionary Instrument vibrating microtome that was made specifically for fixed tissues for histology. Back then it was the VF400 model. It is supposed to be able to cut 10 micron thick sections and we have not tested that it has this capability yet. It's design is simple and it is easy to use. It's not as sexy as some of the other Vibratome units, but it seems to work pretty well. One of our researchers has a model they use for live tissue work and they have had great success with it. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From NLinke <@t> mednet.ucla.edu Tue Nov 3 13:03:14 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Tue Nov 3 13:03:21 2009 Subject: [Histonet] job opening at UCLA Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE49415F89A@EMGMB1.ad.medctr.ucla.edu> Hi all, I have an opening for a Histotech III in the histology lab which will be coming up shortly (it's not posted yet). For this position, an HTL is required, no exceptions. We are looking for someone with 5+ years of experience in routine histology, manual specials, automated special stain experience is always a help, as well as someone with leadership skills. Salary range for this position is $36.83-$47.66 per hour DOE. If you are interested, please let me know! Thanks, Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From srishan <@t> mail.holyname.org Tue Nov 3 13:02:49 2009 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue Nov 3 13:03:56 2009 Subject: [Histonet] regarding validation for the instrument install and antibodies Message-ID: Hello Everyone, Apart from ER/PR,howmany cases/control blocks need to be run for antibody validation? We are talking about commonly used antibodies. Thank you Nirmala Srishan Holy Name Hospital Teaneck, NJ 07666 Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From Lynne.Bell <@t> cvmc.org Tue Nov 3 13:05:08 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Nov 3 13:05:13 2009 Subject: [Histonet] Diff Quik CPT code In-Reply-To: <699556.9372.qm@web81206.mail.mud.yahoo.com> Message-ID: The CPT code for diff-quik for H.pylori is 88313. Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From Jackie.O'Connor <@t> abbott.com Tue Nov 3 13:24:56 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Nov 3 13:25:28 2009 Subject: [Histonet] Arm Rests In-Reply-To: References: Message-ID: Yeah - don't use em. Since I had developed tendinitis in both elbows from microtomy, EHS ergonomics people at a former employer decided to invent some. They covered them with some fake vinyl material, and the very first day I tried using them, my elbow slipped on the shiny vinyl and I cut off the tip of my finger on the blade. I have since learned that resting your forearms on the sharp edge of the bench caused my tendinitis. Since I quit that bad habit, I haven't had a recurrence in 15 years - nor have I cut myself. From: "Weems, Joyce" To: Date: 11/03/2009 12:58 PM Subject: [Histonet] Arm Rests Sent by: histonet-bounces@lists.utsouthwestern.edu Does anyone have a recommendation for microtome arm rests? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kblack <@t> digestivehlth.com Tue Nov 3 15:06:09 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Tue Nov 3 15:06:18 2009 Subject: [Histonet] Diff Quik CPT code References: Message-ID: <0895B03F71B043BB8F05B0144059D6EA@digestivehlth.com> Actually it is 88312 (micro-organisms like Helicobacter pylori). 88313 is for non-organism special stains like iron, collagen etc. except IHC stains. Konni Black Digestive Health Laboratory Tacoma, WA ----- Original Message ----- From: "Bell, Lynne" To: "'Cristi stephenson'" ; Sent: Tuesday, November 03, 2009 11:05 AM Subject: RE: [Histonet] Diff Quik CPT code > The CPT code for diff-quik for H.pylori is 88313. > > Lynne Bell, HT (ASCP) > Lead Histologist > Central Vermont Medical Center > 130 Fisher Road > Barre, VT 05641 > 802-371-4923 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Nov 3 19:23:29 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Nov 3 19:23:41 2009 Subject: [Histonet] Schiff mystery In-Reply-To: <19BB3E7651084F67BCEB6404F35F60B1@HPPav2> Message-ID: Peggy, I would be worried by the activated charcoal. It could be possible that the charcoal clarifying step is removing too much of the active schiff reagent. The students may be trying to obtain a clear solution and mixing the charcoal one more time. I would suggest that they stop once a yellow colour is obtained. I had a similar problem when preparing thionine-schiffs. Activated charcoal removed the active schiffs reagent as well as the contaminants so for a blue schiffs I do not clarify with charcoal. The dehydrating alcohols remove the pink-purple background staining, leaving the positive blue staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, 2 November 2009 9:07 PM To: histonet@lists.utsouthwestern.edu Cc: 'Peggy Wenk' Subject: [Histonet] Schiff mystery Need the help of the Histonet chemistry gurus. This has happened the last 2 times the students and I made up Schiff. Two different set of students. We make up the Schiff, and use it the next day, and it works correctly (stains the glycogen in liver and neutral mucins in intestine a bright magenta). Store in refrig. 1 week later, when we go to stain fungus with PAS, the Schiff doesn't work. Try the same liver and small intestine controls. Doesn't work at all. No magenta, no pink, nothing on the slides. When going into the water rinse after Schiff, the water turns pink with the newly made Schiff, but 1 week later, there is no color change in the water rinse. When we make up the Schiff, we show the students the Schiff quality assessment, where we put a few drops of formaldehyde in it, and the Schiff changes to purple immediately. Works great newly made. One week later, when we can't get the Schiff to stain, there is no color change with formaldehyde. None. It is the same procedure we've been using for years to make up the Schiff. It is the same procedure our histology lab uses. Their Shiff lasts for months. Ours last for a few days. I think it might be one of our reagents is going bad. Histology lab has their own reagents, the School has our own. - The basic fuchsin is OK, as the Kinyoun works great. - The hydrochloric acid was taken from histology, so I'm guessing it's OK. - The charcoal is fairly new. Could that be the problem? Why? - We are using sodium metabisulfite, not sodium sulfite or sulfate. At least that's what the bottle says. Does this break down and go bad? Need all the help we can get. This is spooky, Halloween or no Halloween. Peggy Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Karen.Heckford <@t> CHW.edu Wed Nov 4 07:34:05 2009 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Nov 4 07:34:09 2009 Subject: [Histonet] Having problems posting on Histonet Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697EE3@CHW-MSG-301.chw.edu> I was wondering if anyone else is having problems posting on the histonet. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From leiker <@t> buffalo.edu Wed Nov 4 08:31:52 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Nov 4 08:31:58 2009 Subject: [Histonet] Having problems posting on Histonet In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC105697EE3@CHW-MSG-301.chw.edu> References: <2842DC75AE43AA4B92954CFB31781BC105697EE3@CHW-MSG-301.chw.edu> Message-ID: It worked! Received your post. :-) --On Wednesday, November 04, 2009 6:34 AM -0700 "Heckford, Karen - SMMC-SF" wrote: > I was wondering if anyone else is having problems posting on the > histonet. > > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Rcartun <@t> harthosp.org Wed Nov 4 09:25:26 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Nov 4 09:25:32 2009 Subject: [Histonet] regarding validation for the instrument install and antibodies In-Reply-To: References: Message-ID: <4AF15695.7400.0077.1@harthosp.org> There is no straight-forward answer to question. It really depends on how confident your pathologists are with the results of the particular IHC test. For some proteins you may only need to run three positive and three negative cases. For others you may need to run ten positive and ten negative cases. Keep in mind that doing these validation studies is expensive and time-consuming. Therefore, I always recommend that you continue to add cases (both positive and negative) prospectively (from your daily cases) to your initial validation to increase your numbers. I also believe that laboratories should only do those IHC tests that they will perform on a regular basis, have the expertise to interpret, and the control material in-house to run in parallel with the patient specimen. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 11/3/2009 2:02 PM >>> Hello Everyone, Apart from ER/PR,howmany cases/control blocks need to be run for antibody validation? We are talking about commonly used antibodies. Thank you Nirmala Srishan Holy Name Hospital Teaneck, NJ 07666 Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Wed Nov 4 09:46:46 2009 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Nov 4 09:46:53 2009 Subject: [Histonet] Laboratory Licensing Message-ID: We were recently surveyed by the Joint Commission. We have our laboratory at one physical address and our Dermatopathologists at another physical address (the hospital). The surveyor indicated that we need a separate CLIA and state license for the hospital location where the slides are being read even though no tests are performed there. These are high complexity Tissue Pathology. Has anyone heard of this before or have a similar set up and how are you licensed. It doesn't sound accurate to me. My understanding was that any test being performed required licensure, I have never heard of laboratory licensure being required for the reporting of results. Please provide any assistance or experience you can!!! Thanks! Caroline M. Pratt, MBA Practice Administrator Dermatopathology UPHS 3700 Market Street, Ste 312 Philadelphia, PA 19104 phone 215-349-8178 fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Karen.Heckford <@t> CHW.edu Wed Nov 4 10:02:16 2009 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Nov 4 10:02:22 2009 Subject: [Histonet] Per Diem HT in San Francisco Message-ID: <2842DC75AE43AA4B92954CFB31781BC105697EE5@CHW-MSG-301.chw.edu> We are currently looking for a per diem certified Histology Tech that can also do some Pathology assisting for vacation relief and sick time. Possibly turn into a part time position. Please contact St. Mary's Medical Center in San Francisco website. Thanks, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Lynne.Bell <@t> cvmc.org Wed Nov 4 10:08:25 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Wed Nov 4 10:08:31 2009 Subject: [Histonet] Conventional Tissue Processors Message-ID: We are in the process of purchasing a new tissue processor to replace an aging Tissue-Tek VIP E300. I would love to have opinions on the following processors - Leica ASP300 S, Shandon Excelsior ES, and Tissue-Tek VIP 6. Obviously, reliability is the utmost concern. I would also like to know from those in the New England area how service is for any of these processors. Thank you, Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From DKBoyd <@t> chs.net Wed Nov 4 11:03:24 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Nov 4 11:03:21 2009 Subject: [Histonet] Conventional Tissue Processors In-Reply-To: Message-ID: We have 2 Shandon Excelsiors. We absolutely love them. Great processing and the time saved by not having to rotate reagents is wonderful. We have had very little problems with ours. One is 6 years old and the other is 1.5 years old. The older one has had a few problems that we have attributed to the move (when we moved from an old facility to a new one). The new one hasn't had any problems. Hope this helps. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "Bell, Lynne" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/04/2009 11:10 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Conventional Tissue Processors We are in the process of purchasing a new tissue processor to replace an aging Tissue-Tek VIP E300. I would love to have opinions on the following processors - Leica ASP300 S, Shandon Excelsior ES, and Tissue-Tek VIP 6. Obviously, reliability is the utmost concern. I would also like to know from those in the New England area how service is for any of these processors. Thank you, Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rshooki_99 <@t> yahoo.com Wed Nov 4 11:33:25 2009 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Wed Nov 4 11:33:28 2009 Subject: [Histonet] H&E stainer Message-ID: <623205.79143.qm@web36107.mail.mud.yahoo.com> We are currently looking for a new H&E stainer, i am looking for any and all information? on the current H&E stainers out there in histo-land. thank you.. Richard Shook HT (ASCP) Rabkin Dermatopathology Lab From cmiller <@t> physlab.com Wed Nov 4 12:00:07 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Nov 4 12:00:15 2009 Subject: [Histonet] RE: Laboratory Licensing In-Reply-To: References: Message-ID: I agree with you, what happens when a lab processes only? We in the past have had accounts that we would gross, section, stain and coverslip. We then send completed slides to the submitting Physician usually a Dermatologist who likes to read and diagnose his own slides. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Wednesday, November 04, 2009 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laboratory Licensing Importance: High We were recently surveyed by the Joint Commission. We have our laboratory at one physical address and our Dermatopathologists at another physical address (the hospital). The surveyor indicated that we need a separate CLIA and state license for the hospital location where the slides are being read even though no tests are performed there. These are high complexity Tissue Pathology. Has anyone heard of this before or have a similar set up and how are you licensed. It doesn't sound accurate to me. My understanding was that any test being performed required licensure, I have never heard of laboratory licensure being required for the reporting of results. Please provide any assistance or experience you can!!! Thanks! Caroline M. Pratt, MBA Practice Administrator Dermatopathology UPHS 3700 Market Street, Ste 312 Philadelphia, PA 19104 phone 215-349-8178 fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From trathborne <@t> somerset-healthcare.com Wed Nov 4 12:04:40 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Nov 4 12:04:45 2009 Subject: [Histonet] Muscle biopsies Message-ID: Can anyone recommend a reputable lab (preferable upper-east coast) to send muscle biopsies to? Thanks, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From mcauliff <@t> umdnj.edu Wed Nov 4 12:08:32 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Nov 4 12:06:43 2009 Subject: [Histonet] Compresstome In-Reply-To: <9416F249-A252-4B17-A9CD-CBCA907F4DFA@uiuc.edu> References: <9416F249-A252-4B17-A9CD-CBCA907F4DFA@uiuc.edu> Message-ID: <4AF1C320.2010201@umdnj.edu> I suggest you ask for a demo in your lab with your tissue to see if the machine meets your needs. Geoff Edward Roy wrote: > > At the Society for Neuroscience meeting I saw a variation on > vibratomes called a Compresstome, by Precisionary Instruments. > Has anyone tried this instrument? They claim they can do 10 micron > sections of formaldehyde fixed mouse brain and do free-floating IHC > with them. > Thanks > Ed > > Edward Roy, PhD > Professor of Pathology > Professor of Molecular and Integrative Physiology > University of Illinois at Urbana-Champaign > 506 S. Mathews Ave. > Urbana, IL 61801 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From hymclab.hymclab <@t> ministryhealth.org Wed Nov 4 12:06:35 2009 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Nov 4 12:06:53 2009 Subject: [Histonet] Diff Quik CPT code In-Reply-To: <0895B03F71B043BB8F05B0144059D6EA@digestivehlth.com> References: <0895B03F71B043BB8F05B0144059D6EA@digestivehlth.com> Message-ID: That's what we charge also---88312. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konni Black Sent: Tuesday, November 03, 2009 3:06 PM To: Bell, Lynne; 'Cristi stephenson'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diff Quik CPT code Actually it is 88312 (micro-organisms like Helicobacter pylori). 88313 is for non-organism special stains like iron, collagen etc. except IHC stains. Konni Black Digestive Health Laboratory Tacoma, WA ----- Original Message ----- From: "Bell, Lynne" To: "'Cristi stephenson'" ; Sent: Tuesday, November 03, 2009 11:05 AM Subject: RE: [Histonet] Diff Quik CPT code > The CPT code for diff-quik for H.pylori is 88313. > > Lynne Bell, HT (ASCP) > Lead Histologist > Central Vermont Medical Center > 130 Fisher Road > Barre, VT 05641 > 802-371-4923 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From JPeters <@t> bostwicklaboratories.com Wed Nov 4 12:30:27 2009 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Wed Nov 4 12:30:33 2009 Subject: [Histonet] Victoria Blue stain Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F0755A0A0@mail1.BOSTWICK.COM> One of my pathologists has requested that we start offering a Victoria Blue stain for liver specimens. I am unfamiliar with this stain and was wondering if anyone had a protocol for this that works well? Thanks in advance for any help :-) Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com From cbarone <@t> NEMOURS.ORG Wed Nov 4 12:41:55 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Nov 4 12:42:10 2009 Subject: [Histonet] Not too late to earn CEU's! Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A776F@wlmmsx01.nemours.org> Thank you. From kblack <@t> digestivehlth.com Wed Nov 4 13:02:27 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Wed Nov 4 13:02:37 2009 Subject: [Histonet] RE: Laboratory Licensing References: Message-ID: <48249C5A881E485F895F5BF825B535B8@digestivehlth.com> The "test", according to CLIA, is the reading (interpretation/ diagnosis) of the slide. You do not need a CLIA license to prepare the slides. Konni Black Tacoma, WA ----- Original Message ----- From: "Cheri Miller" To: "Pratt, Caroline" ; Sent: Wednesday, November 04, 2009 10:00 AM Subject: [Histonet] RE: Laboratory Licensing >I agree with you, what happens when a lab processes only? We in the past >have had accounts that we would gross, section, stain and coverslip. We >then send completed slides to the submitting Physician usually a >Dermatologist who likes to read and diagnose his own slides. > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, > Caroline > Sent: Wednesday, November 04, 2009 9:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Laboratory Licensing > Importance: High > > We were recently surveyed by the Joint Commission. We have our > laboratory at one physical address and our Dermatopathologists at > another physical address (the hospital). The surveyor indicated that we > need a separate CLIA and state license for the hospital location where > the slides are being read even though no tests are performed there. > These are high complexity Tissue Pathology. Has anyone heard of this > before or have a similar set up and how are you licensed. It doesn't > sound accurate to me. My understanding was that any test being > performed required licensure, I have never heard of laboratory licensure > being required for the reporting of results. Please provide any > assistance or experience you can!!! Thanks! > > > > Caroline M. Pratt, MBA > > Practice Administrator > > Dermatopathology > > UPHS > > 3700 Market Street, Ste 312 > > Philadelphia, PA 19104 > > phone 215-349-8178 > > fax 215-662-6150 > > > > > > The information contained in this e-mail message is intended only for the > personal and confidential use of the recipient(s) named above. If the > reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> umn.edu Wed Nov 4 13:03:37 2009 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Wed Nov 4 13:03:32 2009 Subject: [Histonet] Victoria Blue stain In-Reply-To: <24D22DE9E488AA43BF92A4389F2DDB1F0755A0A0@mail1.BOSTWICK.CO M> References: <24D22DE9E488AA43BF92A4389F2DDB1F0755A0A0@mail1.BOSTWICK.COM> Message-ID: VICTORIA BLUE - NUCLEAR FAST RED FOR HBSAg PRINCIPLE To demonstrate copper associated protein, elastic and Hepatitis B surface antigen (HBSAg) in paraffin wax sections. REFERENCE . AFIP laboratory methods in Histotechnology p210 Tanaka method Ref:Acta Pathol Jpn 1981;31:93 SPECIMEN 10% neutral buffered formalin. Standard paraffin wax sections. CONTROL Known case of primary biliary cirrhosis. REAGENTS (A) VICTORIA BLUE Distilled water 200 mL Dextrine 0.5 g Victoria Blue 2 g Resorcinol 4 g Gradually warm up the mixed solution of all the above until it boils. Remove form heat then gradually add 25mL of boiling 29% ferric chloride solution (12mL commercial 60% ferric chloride solution plus 13mL distilled water) and boil the solution for a further 3 minutes. Cool. After cooling filter it. Dry the filtrate on the filter paper in a 56?C oven until completely dry. Dissolve the dried filtrate in 400mL of 70% alcohol. Finally add 4mL concentrated hydrochloric acid and 6g phenol. It is best to leave this solution to mature for at least two weeks prior to use. (B) NUCLEAR FAST RED Dissolve 0.5g nuclear fast red in 500mL of warmed 5% aluminium sulphate. Filter when cool. (C) 4% AQUEOUS SODIUM METABISULPHITE PROCEDURE 1. Deparaffinize and hydrate to distilled water. 2. Treat with acidified potassium permanganate (as for Gordon and Sweet's Reticular fibres method 2.4) for 5 minutes. 3. Treat with 4% sodium bisulphite for 1 minute. 4. Wash in running tap water. 5. Wash well with 70% alcohol. 6. Stain in the Victoria Blue solution, in a Coplin jar, for a minimum of 4 hours (preferably overnight). 7. Wash well with 70% alcohol for approx 1 minute. This is the differentiation step - ensure the background of the section is clear. 8. Wash in running tap water for 1 minute. 9. Stain with nuclear fast red solution for 5 minutes. 10. Wash in running water for 2 minutes. 11. Dehydrate, clear and mount. RESULTS HBsAg, elastic fibres, copper associated protein blue All other tissue components pink/red HEALTH AND SAFETY Potassium permanganate Harmful by ingestion. Irritating to eyes and skin Ferric chloride Irritant. Hydrochloric acid Vesicant. Causes burns. Phenol Harmful by ingestion. Irritating to eyes and skin. At 12:30 PM 11/4/2009, Justin Peters wrote: >One of my pathologists has requested that we start offering a Victoria >Blue stain for liver specimens. I am unfamiliar with this stain and was >wondering if anyone had a protocol for this that works well? Thanks in >advance for any help :-) > > > >Justin Peters, HTL (ASCP) > >IHC Supervisor > >Bostwick Laboratories(tm) >For Absolute Confidence(r) > >4355 Innslake Drive >Glen Allen, Virginia 23060 >Phone: (804) 967-9225 ext. 1831 >Cell: (804) 822-6084 >Email: jpeters@bostwicklaboratories.com > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Nov 4 13:50:25 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Nov 4 13:52:33 2009 Subject: [Histonet] Laboratory Licensing References: Message-ID: Haven't heard of that one yet. Just so the Dr. is licensed to read the slides. Wouldn't it essentially (geographically) be the same as a Dr. reading consult slides from another hospital? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pratt, Caroline Sent: Wed 11/4/2009 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laboratory Licensing We were recently surveyed by the Joint Commission. We have our laboratory at one physical address and our Dermatopathologists at another physical address (the hospital). The surveyor indicated that we need a separate CLIA and state license for the hospital location where the slides are being read even though no tests are performed there. These are high complexity Tissue Pathology. Has anyone heard of this before or have a similar set up and how are you licensed. It doesn't sound accurate to me. My understanding was that any test being performed required licensure, I have never heard of laboratory licensure being required for the reporting of results. Please provide any assistance or experience you can!!! Thanks! Caroline M. Pratt, MBA From billingconsultants <@t> yahoo.com Wed Nov 4 14:00:08 2009 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Wed Nov 4 14:00:17 2009 Subject: [Histonet] Re: Arm Rests Message-ID: <575424.12652.qm@web54207.mail.re2.yahoo.com> Hi Joyce, ? One thing we found in?a lab that worked great is tennis balls on the mictrome handle - it's not an arm rest but it seemed to help a lot in changing the?position of?the tech's hand in turning the wheel. Louri Roberts www.peachtreebilling.com From CIngles <@t> uwhealth.org Wed Nov 4 14:12:39 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Nov 4 14:15:15 2009 Subject: [Histonet] storage temps References: Message-ID: Hey gang: Just a simple quiz. What are the temp. ranges for long term slide and (more importantly) paraffin block storage? Claire From RohrT <@t> nyackhospital.org Wed Nov 4 14:29:37 2009 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Wed Nov 4 14:34:47 2009 Subject: [Histonet] Gomori's Retic Stain Message-ID: <10F48667BEE99D4A93EB036B5E71C6C92506FCD7E0@EXVPMBX104-1.exc104.nyackhospital.org> Histonetters. Suddenly having a problem with Retic stain. Fibers weakly staining and dark silver spots deposited all over slide. We seem to be following same protocol we always used but I am wondering if something I am not noticing has changed. Could it be too much Ammonium Hydroxide clearing the precipitate or too little used. Reagents made up new of course and extra care with acid glass cleaning. Just thought maybe someone else had a similar problem and found an easy solution. If you have any thoughts I would be very appreciative. Theresa Rohr, BA, HT Nyack Hospital, NY rohrt@nyackhospital.org Confidentiality Notice: This e-mail message, including any attachment, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ktuttle <@t> umm.edu Wed Nov 4 15:12:05 2009 From: ktuttle <@t> umm.edu (ktuttle@umm.edu) Date: Wed Nov 4 15:12:11 2009 Subject: [Histonet] LABORATORY RESEARCH TECHNICIAN (Baltimore) Message-ID: <20091104211205.EF195C9A0A@web49p.int.craigslist.org> ktuttle@umm.edu has forwarded you this craigslist.org posting. Please see below for more information. Visit the posting at http://baltimore.craigslist.org/sci/1451552794.html to contact the person who posted this. ============================================================ LABORATORY RESEARCH TECHNICIAN Date: 2009-11-04, 3:56PM Processes fresh tissue samples for banking. Retrieves archived paraffin embedded tissue samples, banked tissue samples and slides. Performs cell isolations using sterile procedures. General knowledge of the principles and practices of natural and/or chemical sciences; of the safe and proper handling and disposal of applicable specimens, materials and equipment. Ability to independently perform laboratory procedures using applicable equipment and techniques; to communicate effectively both orally and in writing; to use computers and related software applications; to lift, transport and deliver materials and stock laboratory supplies and goods; to wear and work in personal protective equipment. Histology or Pathology experience is preferred Location: Baltimore Compensation: commensurate with experience $15+/hr Principals only. Recruiters, please don't contact this job poster. Please, no phone calls about this job! Please do not contact job poster about other services, products or commercial interests. Original URL: http://baltimore.craigslist.org/sci/1451552794.html ============================================================ this craigslist posting was forwarded to you by someone using our email-a-friend feature - if you want to prevent these, please go to: http://www.craigslist.org/cgi-bin/te/zlGau9GdARXZ0FGcs9Ga5d2b3NnLkVWbkVmLAAQdJ ============================================================ From annigyg <@t> gmail.com Thu Nov 5 03:18:06 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Nov 5 03:18:12 2009 Subject: [Histonet] Conventional Tissue Processors In-Reply-To: References: Message-ID: I would urge you to advance from your aged but trusted TT E300 to the Tissue Tek VIP6... Sakura RULE!!!! AbuDhabiAnnie 2009/11/4 > We have 2 Shandon Excelsiors. We absolutely love them. Great processing > and the time saved by not having to rotate reagents is wonderful. We have > had very little problems with ours. One is 6 years old and the other is > 1.5 years old. The older one has had a few problems that we have > attributed to the move (when we moved from an old facility to a new one). > The new one hasn't had any problems. > Hope this helps. > > Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical > Center I > 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: > 804-765-5582 I dkboyd@chs.net > > > > > > > > "Bell, Lynne" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 11/04/2009 11:10 AM > > To > "histonet@lists.utsouthwestern.edu" > cc > > Subject > [Histonet] Conventional Tissue Processors > > > > > > > We are in the process of purchasing a new tissue processor to replace an > aging Tissue-Tek VIP E300. I would love to have opinions on the following > processors - Leica ASP300 S, Shandon Excelsior ES, and Tissue-Tek VIP 6. > Obviously, reliability is the utmost concern. I would also like to know > from those in the New England area how service is for any of these > processors. > > Thank you, > > Lynne Bell, HT (ASCP) > Lead Histologist > Central Vermont Medical Center > 130 Fisher Road > Barre, VT 05641 > 802-371-4923 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From annigyg <@t> gmail.com Thu Nov 5 03:19:35 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Nov 5 03:19:40 2009 Subject: [Histonet] H&E stainer In-Reply-To: <623205.79143.qm@web36107.mail.mud.yahoo.com> References: <623205.79143.qm@web36107.mail.mud.yahoo.com> Message-ID: Sakura Sakura Sakura!!! DRS2000 or the magnificent PRISMA AbuDhabiAnnie 2009/11/4 richard shook > We are currently looking for a new H&E stainer, i am looking for any and > all information on the current H&E stainers out there in histo-land. > thank you.. > > Richard Shook HT (ASCP) > Rabkin Dermatopathology Lab > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From lpwenk <@t> sbcglobal.net Thu Nov 5 04:07:33 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Nov 5 04:07:46 2009 Subject: [Histonet] Gomori's Retic Stain In-Reply-To: <10F48667BEE99D4A93EB036B5E71C6C92506FCD7E0@EXVPMBX104-1.exc104.nyackhospital.org> Message-ID: <8F8D0B1C8A164DA2808643DFB8EA3832@HPPav2> Students and I just had a similar problem yesterday! There are lots of reasons why a retic stain could be light, but since you said you followed the same protocol, I thought it might be what happened to us: When mixing the silver nitrate with the ammonium hydroxide, the result was a yellow color, instead of the gunky gray black the occurs when ammonia is added to the silver solution (which eventually clears when more ammonia is added). No matter how much ammonia was being added, it stayed yellowish. The ammonium hydroxide was near the end of the bottle. Since ammonia likes to outgas, that meant there was very little ammonia left in the "liquid". We obtained a new bottle of ammonia, and the stain worked fine. Chemistry-wise (my understanding), silver nitrate (AgNO3)is soluble in water. When ammonium hydroxide (NH4OH)is added, silver hydroxide (AgOH) is made, which is not soluble in water, so becomes the gunky gray-black precipiate (real scientific terms, I know). When more ammonia is added, the silver hydroxide is made into a silver diamine hydroxide (Ag(NH3)2OH), also known as ammoniacal silver. This silver diamine hydroxide is again soluble in water, which is why the gray-black gunk redissolves. If your ammonium hydroxide is weak, then not all the silver hydroxide is converted into silver diamine hydroxide. Therefore, there is not enough silver diamine hydroxide to bind to the reticulin fibers (= light staining). And there is still silver hydroxide in the solution (= precipitate on your tissue/slides). Peggy Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa Rohr Sent: Wednesday, November 04, 2009 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori's Retic Stain Histonetters. Suddenly having a problem with Retic stain. Fibers weakly staining and dark silver spots deposited all over slide. We seem to be following same protocol we always used but I am wondering if something I am not noticing has changed. Could it be too much Ammonium Hydroxide clearing the precipitate or too little used. Reagents made up new of course and extra care with acid glass cleaning. Just thought maybe someone else had a similar problem and found an easy solution. If you have any thoughts I would be very appreciative. Theresa Rohr, BA, HT Nyack Hospital, NY rohrt@nyackhospital.org Confidentiality Notice: This e-mail message, including any attachment, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Nov 5 07:15:32 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Nov 5 07:15:57 2009 Subject: [Histonet] Gomori's Retic Stain In-Reply-To: <8F8D0B1C8A164DA2808643DFB8EA3832@HPPav2> References: <10F48667BEE99D4A93EB036B5E71C6C92506FCD7E0@EXVPMBX104-1.exc104.nyackhospital.org> <8F8D0B1C8A164DA2808643DFB8EA3832@HPPav2> Message-ID: <000001ca5e1a$0ed7e130$2c87a390$@callis@bresnan.net> We always made sure the ammonium hydroxide was fresh stock, and stored this in the refrigerator although the later may not be necessary. If near the expiration date on bottle, NH4OH was replaced. Also, we made sure the silver nitrate solution was fresh, and stored the silver nitrate salts in the refrigerator as directed by the MSDS or as indicated on the bottle. These were two facts picked up from workshops along the years from experts lecturing on the stains. Having fresh silver nitrate was also important for other silver stains e.g. Gomori Methenamine silver for fungus and also Jones Basement Membrane stain. I have seen silver nitrate stored at RT over a long period of time and the salt looked more powdery compared to storing in the refrigerator where the salt was more crystalline in nature. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Thursday, November 05, 2009 3:08 AM To: 'Theresa Rohr'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gomori's Retic Stain Students and I just had a similar problem yesterday! There are lots of reasons why a retic stain could be light, but since you said you followed the same protocol, I thought it might be what happened to us: When mixing the silver nitrate with the ammonium hydroxide, the result was a yellow color, instead of the gunky gray black the occurs when ammonia is added to the silver solution (which eventually clears when more ammonia is added). No matter how much ammonia was being added, it stayed yellowish. The ammonium hydroxide was near the end of the bottle. Since ammonia likes to outgas, that meant there was very little ammonia left in the "liquid". We obtained a new bottle of ammonia, and the stain worked fine. Chemistry-wise (my understanding), silver nitrate (AgNO3)is soluble in water. When ammonium hydroxide (NH4OH)is added, silver hydroxide (AgOH) is made, which is not soluble in water, so becomes the gunky gray-black precipiate (real scientific terms, I know). When more ammonia is added, the silver hydroxide is made into a silver diamine hydroxide (Ag(NH3)2OH), also known as ammoniacal silver. This silver diamine hydroxide is again soluble in water, which is why the gray-black gunk redissolves. If your ammonium hydroxide is weak, then not all the silver hydroxide is converted into silver diamine hydroxide. Therefore, there is not enough silver diamine hydroxide to bind to the reticulin fibers (= light staining). And there is still silver hydroxide in the solution (= precipitate on your tissue/slides). Peggy Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa Rohr Sent: Wednesday, November 04, 2009 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori's Retic Stain Histonetters. Suddenly having a problem with Retic stain. Fibers weakly staining and dark silver spots deposited all over slide. We seem to be following same protocol we always used but I am wondering if something I am not noticing has changed. Could it be too much Ammonium Hydroxide clearing the precipitate or too little used. Reagents made up new of course and extra care with acid glass cleaning. Just thought maybe someone else had a similar problem and found an easy solution. If you have any thoughts I would be very appreciative. Theresa Rohr, BA, HT Nyack Hospital, NY rohrt@nyackhospital.org Confidentiality Notice: This e-mail message, including any attachment, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4574 (20091104) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4574 (20091104) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4574 (20091104) __________ The message was checked by ESET Smart Security. http://www.eset.com From julia.m.hough <@t> hotmail.co.uk Thu Nov 5 09:38:00 2009 From: julia.m.hough <@t> hotmail.co.uk (julia hough) Date: Thu Nov 5 09:38:09 2009 Subject: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. Message-ID: Dear histonetters. I am trying to perform several new IHC stains on mouse tibia. However, I am having trouble finding literature on some of them. I would like antibodies that work on mouse tissue/bone that are preferably conjugated to a fluorchrome. If anyone has any potentially protocols or information where I could get primary antibodies unconjugated/conjugated for the following that would be lovely: N-cadherin, CD146, CD144,ELF97 TRAcP. Thanks for your time. Julia _________________________________________________________________ New Windows 7: Simplify what you do everyday. Find the right PC for you. http://www.microsoft.com/uk/windows/buy/ From jamie.erickson <@t> abbott.com Thu Nov 5 09:59:56 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu Nov 5 10:00:01 2009 Subject: [Histonet] Chitosan in mounting media? Message-ID: Does anyone know of a aqueous mounting media that contains Chitosan ? I have a protocol for frozen sections that suggested using Chitosan to coverslip the sections but they did not give the catalog number or how it was made. Sigma sells it but there are many types, you see my problem. Any help would be appreciated.. Jamie Jamie E Erickson Scientist II, M.S. HTL (ASCP) GPRD Discovery Safety, Metabolism & Pharmacokinetics Abbott 100 Research Dr. Worcester, MA 01607 Phone 508-688-3134 FAX 508-793-4895 jamie.erickson@abbott.com From anonwums1 <@t> gmail.com Thu Nov 5 10:02:02 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Nov 5 10:02:08 2009 Subject: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. In-Reply-To: References: Message-ID: <858249120911050802s6bbd8a2dkcefe80db5534c48a@mail.gmail.com> I use rabbit anti-human N-Cadherin (clone YS from IBL), and it stains mouse bone sections and also mouse cells by Western blot. In my hands, it works great on unretrieved sections fixed with 10% zinc buffered formalin fixed sections (but not neutral buffered formalin or paraformaldehyde fixed sections even with antigen retrieval). I don't have it directly conjugated, but with indirect immunofluorescence, the cells are quite positive so you may be able to directly conjugate it yourself and get a decent signal. Adam On Thu, Nov 5, 2009 at 9:38 AM, julia hough wrote: > > > > Dear histonetters. > > > > I am trying to perform several new IHC stains on mouse > tibia. However, I am having trouble finding literature on some of them. I > would > like antibodies that work on mouse tissue/bone that are preferably > conjugated > to a fluorchrome. > > > > If anyone has any potentially protocols or information where > I could get primary antibodies unconjugated/conjugated for the following > that > would be lovely: > > > > N-cadherin, CD146, CD144,ELF97 TRAcP. > > > > Thanks for your time. > > > > Julia > > > _________________________________________________________________ > New Windows 7: Simplify what you do everyday. Find the right PC for you. > > http://www.microsoft.com/uk/windows/buy/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anonwums1 <@t> gmail.com Thu Nov 5 10:49:22 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Nov 5 10:49:28 2009 Subject: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. In-Reply-To: <858249120911050844j55a7a706v1c4f021e09f937b5@mail.gmail.com> References: <858249120911050802s6bbd8a2dkcefe80db5534c48a@mail.gmail.com> <858249120911050844j55a7a706v1c4f021e09f937b5@mail.gmail.com> Message-ID: <858249120911050849n4d0b291bl416ece2b59ab0057@mail.gmail.com> More info, as Gayle rightly pointed out that I left some out. I fixed in 10% zinc buffered formalin overnight at 4C. I decalcified in dilute formic acid (it's called Immunocal from DeCal corporation) for 72 hours at 4C. They were embedded in paraffin. I use 5 ug / mL of the primary antibody, and then come in with a secondary (1 ug / mL) labeled with a fluorophore. It works great. I have tried it in paraffin embedded 4% PFA fixed, EDTA decalcified sections (with or without antigen retrieval) to no avail. I think that's probably due to the PFA and not the EDTA. I haven't tried frozens yet because paraffins work so well. Adam On Thu, Nov 5, 2009 at 10:44 AM, Adam . wrote: > Hi Julia, > > Presumably, the anti-human stains mouse tissue because of protein homology > between the human N-cadherin and mouse N-cahderin. However, I should note > that there is evidence that this anti-human N-cadherin may cross react with > some other cadherin on mouse tissue and thus the staining may not be > completely specific. > It's buried somewhere in that paper but they incubated the sections with > excess of a different N-cadherin antibody (called MNCD2) and that didn't > block the staining. However, that MNCD2 antibody they used for blocking > probably also is cross reactive (I can give you references and actually seen > that in my own hands) and really, the gold standard to show specificity for > an antibody is to add excess N-cadherin to the antibody prior to incubation > and showing it doesn't bind to tissue antigen anymore (alternatively, you > could stain a knockout mouse, but those guys are lethal). > > IBL is a company.http://www.ibl-america.com/newproducts.html > > Yeah, the nomenclature for zinc is confusing. *10% zinc buffered formalin*is 10% formaldehyde (formalin) with some methanol and also zinc salts added. > Apparently, the zinc ions bind to proteins and prevent them from > cross-linking during fixation... if you use neutral buffered formalin, you > get full cross linking and have to do antigen retrieval. We get the stuff > from Fisher. The recipe you showed is *zinc fixative*, which is something > completely different. That also prevents cross-linking. The stuff is cheap, > so we just buy it in big jugs. > > Adam > > > On Thu, Nov 5, 2009 at 10:22 AM, julia hough wrote: > >> Hi Adam >> >> Thanks a lot for your response. >> >> >> I've not been doing IHC for v. long as I've only recently graduated from >> university, so I hope you won't mind me asking this, but how does anti-human >> stain mouse tissue? I thought it would have to be anti-mouse? >> Also what is the acronym IBL stand for? >> >> For the zinc buffered formalin is this the zinc formalin or the zinc >> fixative as shown below, as I know there is sometimes confusion with this >> histonet. >> >> >> >> *Zinc Fixative* >> >> * >> * Calcium Acetate ---------------------- 0.5 g >> Zinc Acetate -------------------------- 5.0 g >> Zinc Chloride -------------------------- 5.0 g >> 0.1M Tris Buffer made above ------ 1000 ml >> >> >> >> If you?ve got any protocols that?d be lovely. >> >> >> >> Thanks again >> >> >> >> Best wishes >> >> >> >> Julia >> >> >> ------------------------------ >> Date: Thu, 5 Nov 2009 10:02:02 -0600 >> Subject: Re: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. >> From: anonwums1@gmail.com >> To: julia.m.hough@hotmail.co.uk >> CC: histonet@lists.utsouthwestern.edu >> >> I use rabbit anti-human N-Cadherin (clone YS from IBL), and it stains >> mouse bone sections and also mouse cells by Western blot. In my hands, it >> works great on unretrieved sections fixed with 10% zinc buffered formalin >> fixed sections (but not neutral buffered formalin or paraformaldehyde fixed >> sections even with antigen retrieval). I don't have it directly conjugated, >> but with indirect immunofluorescence, the cells are quite positive so you >> may be able to directly conjugate it yourself and get a decent signal. >> >> Adam >> >> On Thu, Nov 5, 2009 at 9:38 AM, julia hough wrote: >> >> >> >> >> Dear histonetters. >> >> >> >> I am trying to perform several new IHC stains on mouse >> tibia. However, I am having trouble finding literature on some of them. I >> would >> like antibodies that work on mouse tissue/bone that are preferably >> conjugated >> to a fluorchrome. >> >> >> >> If anyone has any potentially protocols or information where >> I could get primary antibodies unconjugated/conjugated for the following >> that >> would be lovely: >> >> >> >> N-cadherin, CD146, CD144,ELF97 TRAcP. >> >> >> >> Thanks for your time. >> >> >> >> Julia >> >> >> _________________________________________________________________ >> New Windows 7: Simplify what you do everyday. Find the right PC for you. >> >> http://www.microsoft.com/uk/windows/buy/_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> Download Messenger onto your mobile for free. Learn more. >> > > From anonwums1 <@t> gmail.com Thu Nov 5 12:10:34 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Nov 5 12:10:39 2009 Subject: [Histonet] Cutting paraffin sections in a warm room Message-ID: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> Hi all, I've just recently started cutting paraffin sections (of bones). A few weeks ago, the facilities people decided that it was fall and now the lab is significantly warmer than it used to be, and my paraffin is falling apart and sticking to everything. Apparently, we can't control the temperature through a thermostat at all, and the microtome is inside the lab. I was wondering if anyone had any ideas on how to section in a warm room. Thanks, Adam From LSebree <@t> uwhealth.org Thu Nov 5 12:17:08 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Nov 5 12:17:12 2009 Subject: [Histonet] Cutting paraffin sections in a warm room In-Reply-To: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF657@UWHC-MAIL01.uwhis.hosp.wisc.edu> Adam, You may very well be dealing with static electricity from the drier air produced when the heat gets turned on. Go to the Archives for helpful hints on dealing with this issue. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, November 05, 2009 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting paraffin sections in a warm room Hi all, I've just recently started cutting paraffin sections (of bones). A few weeks ago, the facilities people decided that it was fall and now the lab is significantly warmer than it used to be, and my paraffin is falling apart and sticking to everything. Apparently, we can't control the temperature through a thermostat at all, and the microtome is inside the lab. I was wondering if anyone had any ideas on how to section in a warm room. Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WBENTON <@t> umm.edu Thu Nov 5 12:20:45 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Thu Nov 5 12:21:13 2009 Subject: [Histonet] Muscle Biopsies In-Reply-To: References: Message-ID: <4AF2D12C.D886.00F4.3@umm.edu> Toni, We perform muscle biopsies here at the University of Maryland Medical Center. If you are interested, please contact me and I can give you the information to start the process of becoming a client. Message: 2 Date: Wed, 4 Nov 2009 13:04:40 -0500 From: "Rathborne, Toni" Subject: [Histonet] Muscle biopsies To: "Histonet_Listserv (E-mail)" Message-ID: Content-Type: text/plain; charset="utf-8" Can anyone recommend a reputable lab (preferable upper-east coast) to send muscle biopsies to? Thanks, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From gayle.callis <@t> bresnan.net Thu Nov 5 12:26:04 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Nov 5 12:26:30 2009 Subject: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. In-Reply-To: <858249120911050849n4d0b291bl416ece2b59ab0057@mail.gmail.com> References: <858249120911050802s6bbd8a2dkcefe80db5534c48a@mail.gmail.com> <858249120911050844j55a7a706v1c4f021e09f937b5@mail.gmail.com> <858249120911050849n4d0b291bl416ece2b59ab0057@mail.gmail.com> Message-ID: <001501ca5e45$70508540$50f18fc0$@callis@bresnan.net> Adam, Thank you telling how you prepared bone for N-Cadherin IHC. I am not sure there will be success using any fixative with formaldehyde in the recipe or Paraformaldehyde with the murine CD146, CD144 though. I suggest Julia go to BD Biosciences, SEROTEC, and eBiosciences websites to pick up the IHC applications and fixation needs for these murine CD markers, often picky and not stainable after zinc formalin, NBF or PFA. Start with one company, and if they don't have the antibody, then try the next. Company technical data sheets should tell her if these antibodies will stain formalin fixation and/or only on frozen sections of bone, unfixed. If aldehyde fixatives work, she may have to protect the antigens further by using EDTA instead of an acid decalcifier. Molecular Probes has the kits and protocols available for ELF 97, also Chemicon - and their company technical services will help on line. I believe the TRAcP was recently and has been discussed often in the past on Histonet. A search Histonet Archives should provide more answers. I wish her luck on immunostaining success, often tough when you can't stain for all the antibodies and possibly perform ELF 97 or TRAcP on a single sample if fixation and/or decalcification compromise(s) the results. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, November 05, 2009 9:49 AM To: julia hough; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. More info, as Gayle rightly pointed out that I left some out. I fixed in 10% zinc buffered formalin overnight at 4C. I decalcified in dilute formic acid (it's called Immunocal from DeCal corporation) for 72 hours at 4C. They were embedded in paraffin. I use 5 ug / mL of the primary antibody, and then come in with a secondary (1 ug / mL) labeled with a fluorophore. It works great. I have tried it in paraffin embedded 4% PFA fixed, EDTA decalcified sections (with or without antigen retrieval) to no avail. I think that's probably due to the PFA and not the EDTA. I haven't tried frozens yet because paraffins work so well. Adam On Thu, Nov 5, 2009 at 10:44 AM, Adam . wrote: > Hi Julia, > > Presumably, the anti-human stains mouse tissue because of protein homology > between the human N-cadherin and mouse N-cahderin. However, I should note > that there is evidence that this anti-human N-cadherin may cross react with > some other cadherin on mouse tissue and thus the staining may not be > completely specific. > It's buried somewhere in that paper but they incubated the sections with > excess of a different N-cadherin antibody (called MNCD2) and that didn't > block the staining. However, that MNCD2 antibody they used for blocking > probably also is cross reactive (I can give you references and actually seen > that in my own hands) and really, the gold standard to show specificity for > an antibody is to add excess N-cadherin to the antibody prior to incubation > and showing it doesn't bind to tissue antigen anymore (alternatively, you > could stain a knockout mouse, but those guys are lethal). > > IBL is a company.http://www.ibl-america.com/newproducts.html > > Yeah, the nomenclature for zinc is confusing. *10% zinc buffered formalin*is 10% formaldehyde (formalin) with some methanol and also zinc salts added. > Apparently, the zinc ions bind to proteins and prevent them from > cross-linking during fixation... if you use neutral buffered formalin, you > get full cross linking and have to do antigen retrieval. We get the stuff > from Fisher. The recipe you showed is *zinc fixative*, which is something > completely different. That also prevents cross-linking. The stuff is cheap, > so we just buy it in big jugs. > > Adam > > > On Thu, Nov 5, 2009 at 10:22 AM, julia hough wrote: > >> Hi Adam >> >> Thanks a lot for your response. >> >> >> I've not been doing IHC for v. long as I've only recently graduated from >> university, so I hope you won't mind me asking this, but how does anti-human >> stain mouse tissue? I thought it would have to be anti-mouse? >> Also what is the acronym IBL stand for? >> >> For the zinc buffered formalin is this the zinc formalin or the zinc >> fixative as shown below, as I know there is sometimes confusion with this >> histonet. >> >> >> >> *Zinc Fixative* >> >> * >> * Calcium Acetate ---------------------- 0.5 g >> Zinc Acetate -------------------------- 5.0 g >> Zinc Chloride -------------------------- 5.0 g >> 0.1M Tris Buffer made above ------ 1000 ml >> >> >> >> If you've got any protocols that'd be lovely. >> >> >> >> Thanks again >> >> >> >> Best wishes >> >> >> >> Julia >> >> >> ------------------------------ >> Date: Thu, 5 Nov 2009 10:02:02 -0600 >> Subject: Re: [Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP. >> From: anonwums1@gmail.com >> To: julia.m.hough@hotmail.co.uk >> CC: histonet@lists.utsouthwestern.edu >> >> I use rabbit anti-human N-Cadherin (clone YS from IBL), and it stains >> mouse bone sections and also mouse cells by Western blot. In my hands, it >> works great on unretrieved sections fixed with 10% zinc buffered formalin >> fixed sections (but not neutral buffered formalin or paraformaldehyde fixed >> sections even with antigen retrieval). I don't have it directly conjugated, >> but with indirect immunofluorescence, the cells are quite positive so you >> may be able to directly conjugate it yourself and get a decent signal. >> >> Adam >> >> On Thu, Nov 5, 2009 at 9:38 AM, julia hough wrote: >> >> >> >> >> Dear histonetters. >> >> >> >> I am trying to perform several new IHC stains on mouse >> tibia. However, I am having trouble finding literature on some of them. I >> would >> like antibodies that work on mouse tissue/bone that are preferably >> conjugated >> to a fluorchrome. >> >> >> >> If anyone has any potentially protocols or information where >> I could get primary antibodies unconjugated/conjugated for the following >> that >> would be lovely: >> >> >> >> N-cadherin, CD146, CD144,ELF97 TRAcP. >> >> >> >> Thanks for your time. >> >> >> >> Julia >> >> >> _________________________________________________________________ >> New Windows 7: Simplify what you do everyday. Find the right PC for you. >> >> http://www.microsoft.com/uk/windows/buy/____________________________________ ___________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> Download Messenger onto your mobile for free. Learn more. >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4576 (20091105) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4576 (20091105) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4576 (20091105) __________ The message was checked by ESET Smart Security. http://www.eset.com From rsrichmond <@t> gmail.com Thu Nov 5 12:36:47 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Nov 5 12:36:53 2009 Subject: [Histonet] Re: Muscle biopsies Message-ID: Toni Rathborne, Pathology Supervisor, Somerset Medical Center, Somerville NJ asks: >>Can anyone recommend a reputable lab (preferable upper-east coast) to send muscle biopsies to?<< The preparation of muscle biopsy specimens is very exacting - they need to be transported on wet ice, quite unfixed - you need to find a facility within a reasonable driving distance, and work out transport arrangements with them, and make sure the specimen is obtained in time to get it transported and received promptly (I've on occasion enlisted family members to do this.) You and your pathologist need to work all this out in advance of need. Let us all know how this process works out for you. Bob Richmond Samurai Pathologist Knoxville TN From gayle.callis <@t> bresnan.net Thu Nov 5 12:42:22 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Nov 5 12:42:47 2009 Subject: [Histonet] Cutting paraffin sections in a warm room In-Reply-To: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> References: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> Message-ID: <000001ca5e47$b786e380$2694aa80$@callis@bresnan.net> Adam, Cool trimmed bone blocks on a block of ice with extra water on top of ice. This helps soften the bone and keepS the block cold but don't over soak, that can lead to shredded sections. A cube of ice wrapped in gauze, held to face of block and also held to knife holder may help or simply keep a wad of gauze on the ice block, and hold to block face just before continuing to section a block. We even put the forceps used to grasp ribbon cold in an ice bath, prevents sticking paraffin and keep the microtome blade holder (metal plate) clean - paraffin likes to stick to everything, even itself. We also infiltrate and embed bone in a harder paraffin (Tissue Prep 2) to match the harder matrix of decalcified bone to paraffin. There have been times when our lab was stifling, and cooling the trimmed block on ice block solved the problem. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, November 05, 2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting paraffin sections in a warm room Hi all, I've just recently started cutting paraffin sections (of bones). A few weeks ago, the facilities people decided that it was fall and now the lab is significantly warmer than it used to be, and my paraffin is falling apart and sticking to everything. Apparently, we can't control the temperature through a thermostat at all, and the microtome is inside the lab. I was wondering if anyone had any ideas on how to section in a warm room. Thanks, Adam __________ Information from ESET Smart Security, version of virus signature database 4576 (20091105) __________ The message was checked by ESET Smart Security. http://www.eset.com From cbrya <@t> lexclin.com Thu Nov 5 12:53:39 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Nov 5 12:53:45 2009 Subject: [Histonet] workflow Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF17A22BE3@EXCHANGESB> We currently have 3 histotechs at our lab. They arrive at 5 am, 7 am, and 8:30 am. The first tech embeds ? of the workload and is supposed to cut ? of the workload. They typically complete a couple of cases or 1 tray by 8 am. The second tech also embeds ? of the work and cuts ?. The third tech relieves the 5 am person from cutting. Our pathologists are receiving one tray of slides at 8 am and then all the work comes off the stainer by 10:00 am. They would like to have more slides by 8 am. As the schedule stands they are having 1 hour of waiting time for slides. I am trying to decide the most efficient way to get the work out to the pathologists by 8 am. Our caseload is around 90 blocks per day and we have mostly small biopsy specimens. Do most labs have someone embed everything first and then other techs cutting? We didn't want our techs to lose their embedding skills. Is 3 hours enough time for the first shift to embed, cut & stain more than 1 rack? Any input would be greatly appreciated. Thank you in advance. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From marsh2 <@t> niehs.nih.gov Thu Nov 5 14:08:04 2009 From: marsh2 <@t> niehs.nih.gov (Marsh, Tiwanda (NIH/NIEHS) [E]) Date: Thu Nov 5 14:08:43 2009 Subject: [Histonet] Light Box to quench autofluorescence Message-ID: Hello, I've received information from several sources saying that using a light box is a good way to quench autofluorescence for IF. Does anyone know where I can purchase one of these? Thanks From Vickroy.Jim <@t> mhsil.com Thu Nov 5 14:11:25 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Nov 5 14:11:32 2009 Subject: [Histonet] "Processing" small gi biopsies, skin lesions, currettings Message-ID: <24A4826E8EF0964D86BC5317306F58A5425377F903@mmc-mail.ad.mhsil.com> I am in the process of reviewing our procedures in the gross room. Currently we have a few techs that do these small biopsies. Each one actually has a bachelor of science degree along with their HT certification. The number of these folks is shrinking and I want to include some other histotechs that do not have an associates or bachelor's degree. Since these specimens now come under "processing" instead of "gross descriptions" , and are not defined as "high complexity testing (CLIA), I believe that I can have other histotechs perform this task as long as certain rules are followed such as: 1. Defining the nature of the pathologist supervision clearly for each type of specimen 2. Monitoring the performance on a regular, periodic basis 3. Written procedures for "processing" specimens. Is this everyone else understanding? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From arvidsonkristen <@t> yahoo.com Thu Nov 5 14:22:16 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Thu Nov 5 14:22:20 2009 Subject: [Histonet] lab spill clean-up Message-ID: <812807.9570.qm@web65707.mail.ac4.yahoo.com> I am looking for quantities of various lab chemicals that are considered safe for lab personnel to clean up.? Are there set guidelines for when you should call for help? From Jackie.O'Connor <@t> abbott.com Thu Nov 5 14:25:50 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Nov 5 14:26:23 2009 Subject: [Histonet] lab spill clean-up In-Reply-To: <812807.9570.qm@web65707.mail.ac4.yahoo.com> References: <812807.9570.qm@web65707.mail.ac4.yahoo.com> Message-ID: I've always been in institutions (hey, no jokes) where more than a liter of spilled formalin, alcohol, xylene, etc is cleaned up by the in house haz mat people. From: kristen arvidson To: histonet Date: 11/05/2009 02:22 PM Subject: [Histonet] lab spill clean-up Sent by: histonet-bounces@lists.utsouthwestern.edu I am looking for quantities of various lab chemicals that are considered safe for lab personnel to clean up. Are there set guidelines for when you should call for help? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deliadfam <@t> yahoo.com Thu Nov 5 14:31:39 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Thu Nov 5 14:31:43 2009 Subject: [Histonet] "Processing" small gi biopsies, skin lesions, currettings In-Reply-To: <24A4826E8EF0964D86BC5317306F58A5425377F903@mmc-mail.ad.mhsil.com> Message-ID: <232086.67590.qm@web63107.mail.re1.yahoo.com> Thanks for throwing this one out there. I am also interested in this topic. Can you please post replies to the net? Thanks !!! :-) --- On Thu, 11/5/09, Vickroy, Jim wrote: From: Vickroy, Jim Subject: [Histonet] "Processing" small gi biopsies, skin lesions, currettings To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, November 5, 2009, 1:11 PM I am in the process of reviewing our procedures in the gross room.???Currently we have a few techs that do these small biopsies.???Each one actually has a bachelor of science degree along with their HT certification.? The number of these folks is shrinking and I want to include some other histotechs that do not have an associates or bachelor's degree.???Since these specimens now come under "processing" instead of "gross descriptions" , and are not defined as "high complexity testing (CLIA),? I believe that? I can have other histotechs perform this task as long as certain rules are followed? such as: 1.? ? ???Defining the nature of the pathologist supervision clearly for each type of specimen 2.? ? ???Monitoring the performance on a regular, periodic basis 3.? ? ???Written procedures for "processing" specimens. Is this everyone else understanding? James Vickroy BS, HT(ASCP) Surgical? and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Nov 5 15:00:15 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Nov 5 15:00:44 2009 Subject: [Histonet] Looking for Ray Ortiz In-Reply-To: <812807.9570.qm@web65707.mail.ac4.yahoo.com> References: <812807.9570.qm@web65707.mail.ac4.yahoo.com> Message-ID: Can someone tell Ray to contact me? Thanks. From Maria.Katleba <@t> stjoe.org Thu Nov 5 15:16:12 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Nov 5 15:16:21 2009 Subject: [Histonet] OSCAR antibody Message-ID: What do the letters that make up the name OSCAR mean ? There is a debate in my lab over it? :) Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From carrolpb <@t> umdnj.edu Thu Nov 5 15:20:08 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Nov 5 15:20:18 2009 Subject: [Histonet] OSCAR antibody In-Reply-To: References: Message-ID: <4AF34188.6090508@umdnj.edu> osteoclast-associated receptor Maria Katleba wrote: > What do the letters that make up the name OSCAR mean ? There is a debate in my lab over it? :) > > > > Maria Katleba HT(ASCP), MS > Pathology Dept. Mgr. > Queen of the Valley Medical Center > 707-294-9229 cell > 707-252-4411 x3689 > > > > ________________________________ > Notice from St. Joseph Health System: > Please note that the information contained in this message may be privileged and confidential and protected from disclosure. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From michelle-griffin <@t> uiowa.edu Thu Nov 5 15:21:54 2009 From: michelle-griffin <@t> uiowa.edu (Griffin, Michelle A) Date: Thu Nov 5 15:21:57 2009 Subject: [Histonet] Whole mount porcine trachea staining Message-ID: Would anyone have an idea about how to stain whole porcine trachea so that the cartilage rings show prominently? There have been several excellent articles that use Alcian Blue or an Alcian Blue/Alizarin Red S combination to stain rodent tracheas (mouse/rat) and the pictures are wonderful (Schipani et al., 2001; Regnier et al., 2002; Dolle et al., 1993). However, I have tried to reproduce the results using the same protocols just a different species and am having no luck (I did try out each method with some mouse tracheas in my lab and the results mimicked what I saw in published articles). I have tried different digestion/clearing solutions, times, pH, etc and continue to end up with a trachea that is quite ugly with no differentiation between cartilage rings and connective tissue. Does anyone have a suggestion on why pig trachea would not stain even similar to mouse trachea? Any suggestions would be greatly appreciated. Thanks so much for your time and input. Michelle A. Griffin BA, BS, MHA University of Iowa Comparative Pathology Laboratory 200 Hawkins Drive 143 Medical Research Center Iowa City, IA 52242 319-384-4620 From kelly <@t> phenopath.com Thu Nov 5 15:38:03 2009 From: kelly <@t> phenopath.com (Kelly Turner) Date: Thu Nov 5 15:38:49 2009 Subject: [Histonet] OSCAR antibody In-Reply-To: <4AF34188.6090508@umdnj.edu> Message-ID: Patti Loykasek responded to that question a few weeks back: "Actually the name stands for Our Second Cytokeratin Antibody Rocks! A bit of path humor (or at least Dr. Gown tried to be humorous!). We are truly a bit silly sometimes." Kelly Turner, HTL(ASCP)QIHC PhenoPath Laboratories Seattle, WA On 11/5/09 1:20 PM, "Peter Carroll" wrote: > osteoclast-associated receptor > > Maria Katleba wrote: >> What do the letters that make up the name OSCAR mean ? There is a debate in >> my lab over it? :) >> >> >> >> Maria Katleba HT(ASCP), MS >> Pathology Dept. Mgr. >> Queen of the Valley Medical Center >> 707-294-9229 cell >> 707-252-4411 x3689 >> >> >> >> ________________________________ >> Notice from St. Joseph Health System: >> Please note that the information contained in this message may be privileged >> and confidential and protected from disclosure. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From laurie <@t> conxis.com Thu Nov 5 16:09:40 2009 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Thu Nov 5 16:09:51 2009 Subject: [Histonet] cryopreservation of rodent nerves for frozen sections Message-ID: Hello everyone, Happy Thursday. I am working on a project where the investigator is doing immunostaining on thoracic cranial nerves in rat on frozen sections. The initial sections were too riddled with ice artifact to be useable so we are trying again and I am doing sucrose cryopreservation on the nerves, with reduced timing because of sample size. My question is when the sucrose solution is prepped is molecular grade sucrose necessary for this or do people generally use table sugar ( I know... gasp ). Just wondering... Thank you! Laurie From Marilyn.A.Weiss <@t> kp.org Thu Nov 5 18:01:20 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Nov 5 18:01:51 2009 Subject: [Histonet] marilyn weiss will be out of the office as of 6 a.m.11/5/09 returningMonday 11/9 Message-ID: I will be out of the office starting 11/05/2009 and will not return until 11/09/2009. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From lpaveli1 <@t> hurleymc.com Thu Nov 5 19:39:56 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Nov 5 19:40:25 2009 Subject: [Histonet] lab spill clean-up Message-ID: <4AF33824020000EE0002E287@smtp-gw.hurleymc.com> Our institution had an after hours spill of xylene (approx. 1/2 gallon). People panicked, and, long story short, called Hazmat. Of course, it was SuperBowl weekend, and it took them 3 hrs to go the distance of 1 hr. Our lab, clinical lab and emergency was closed down awaiting the clean-up (can't do blood work when the lab is closed). The guys arrived......dressed to the nines in their suits and masks and cleaned up our 1/2 gallon of xylene. When they were leaving, they suggested that anything UNDER 5 gallons should be cleaned up by our institution. Any more, and call them. That's the story, and I'm a stickin' to it!! One good outcome........we switched to ProPar!!! Have a great weekend! >>> Jackie M O'Connor 11/05/09 3:25 PM >>> I've always been in institutions (hey, no jokes) where more than a liter of spilled formalin, alcohol, xylene, etc is cleaned up by the in house haz mat people. From: kristen arvidson To: histonet Date: 11/05/2009 02:22 PM Subject: [Histonet] lab spill clean-up Sent by: histonet-bounces@lists.utsouthwestern.edu I am looking for quantities of various lab chemicals that are considered safe for lab personnel to clean up. Are there set guidelines for when you should call for help? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Nov 6 00:39:21 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Nov 6 00:39:26 2009 Subject: [Histonet] Cutting paraffin sections in a warm room In-Reply-To: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> References: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> Message-ID: One of the little things I learned along the way regarding bone sectioning: if time allows, trim (face) the blocks and leave them in the deep freeze (-?20) overnight. T Then when you are ready to section, take ONE block out at a time and place on ice. this trick is especially useful for us as we cut 50 serial sections at one go. The block is usually cold enough to get at least 40 sections off it before recooling. BTW, we use the deepe embedding moulds, about 1cm deep, so if you are using the flatter mouds, maybe overnight is not necessary my 2c worth have a great weekend! On Thu, Nov 5, 2009 at 8:10 PM, Adam . wrote: > Hi all, > > I've just recently started cutting paraffin sections (of bones). A few > weeks > ago, the facilities people decided that it was fall and now the lab is > significantly warmer than it used to be, and my paraffin is falling apart > and sticking to everything. Apparently, we can't control the temperature > through a thermostat at all, and the microtome is inside the lab. I was > wondering if anyone had any ideas on how to section in a warm room. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lpwenk <@t> sbcglobal.net Fri Nov 6 04:14:08 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Nov 6 04:14:21 2009 Subject: [Histonet] lab spill clean-up In-Reply-To: <812807.9570.qm@web65707.mail.ac4.yahoo.com> Message-ID: <45C748A9C53B4CAB9C84EAAE70FFE0ED@HPPav2> As far as I know, there are no federal regulations as to the amount someone can clean up. It's about safety for the person cleaning it up. At our institution, for most employees, it's the size of 1 container of reagent that could get spilled, which corresponds very nicely to one container of spill absorbent/neutralizer. Formalin - 1 gallon. Solvents (alcohol, acetone, xylene) - We could do 1 gallon (need 4 containers of spill absorbent), but since the spill absorbent only comes in 1 quart size, that's why we say 1 quart (500 mL) is the limit for most employees cleaning it up. Bleach (chlorine) - 1 quart (500 mL) Acids - 500 mL Bases/Caustic (ammonia, sodium hydroxide, etc.) - 500mL (Warning - editorial to follow:) That being said, as Safety Officer, I have a respirator with additional filters for various chemicals, so I get to clean up more volume. But if it's more than I think I can safely handle, we call in an outside company first. They pick up our hazardous chemicals and waste formalin/tissues, so they have training and the respirators and the suits to handle bigger spills. If it's too big for them, then we go with the county's hazmat team. Some of the problem with saying a lab employee can clean up 5 gallons is: - time it takes (hours) for 1 person to clean up - downtime in lab with everyone standing out in the hall until it's all cleaned up (hours) - amount of vapors in the room (even with telling facilities management to boost ventilation to maximum). I would not want someone cleaning up 5 gallons for formalin for hours, and breathing in all the vapors. They would need a respirator with formalin filters. - where do you dispose of 5 gallons of chemical with 5 gallons of neutralizer? For example: I got called into endoscopy area, after hours, for a 5 gallon Metricide (2.5% gluteraldehyde used to clean endoscopy instruments) spill clean up. That took me hours, and in retrospect, I wish I had pulled in the outside company. - 5 gallons of formalin neutralizer would neutralized the 5 gallons of Metricide, but it was too soupy to scoop up. So I added twice as much (actually a total of 12 gallons of Poly-form-F), to thicken it, so I could scoop it. Do most labs have 5-10 gallons of neutralizers around? I had to borrow from many labs, and pray that no one spilled any formalin until the order for more came in. - The little scraper and bags that come with kits were obviously too small to handle the job. (Think emptying out a large sandbox with a kid size pail and scooper.) I got a large broom and a large dust pan with a long handle from housekeeping. (Now think emptying the large sandbox with a regular size shovel. Still would take a couple of hours.) These had to be properly disposed of afterwards. - The plastic bag that comes with the kit won't hold more than 1 gallon of spill with 1 gallon of neutralizer. I figured out that I had available an empty 50 gallon waste barrel lined with a large thick plastic bag (used to dispose tissues/formalin from histology). So I scooped up the 5 gallons of Metricide and 12 gallons of neutralizer into the barrel. I don't know what we would have done if our waste barrels hadn't been swapped out that day, so that there was an empty barrel I could use. Where would your lab put 10+ gallons of spill/neutralizer for disposal? - Spilled occurred about 7 pm. I was contacted 8:30 pm. (long story, it's not even my responsibility to have cleaned it up.) I started cleaning at 9:30 pm, took me until 1:00 am to clean up the spill, and then housekeeping had to mop the floors over and over again, and clean mats, until 3 am. So imagine 6 hours of downtime if this had taken place during the day. So a team of 2-4 could have gotten it cleaned up in 1-2 hours. - I had changed into scrubs to clean it up, and put on my regular clothes to drive home. But I reeked for hours afterwards. I went home and took a long shower and shampoo. I think it got into my pores, as I could still smell it on me afterwards. The clothes were put in the washer immediately, as they smelled, and they were only on me for the 45 minutes it took to drive home. I really would have liked a spaceman's suit. - I had someone outside the room, watching me through a glass window in a door, making certain I was OK. That meant the hospital payed someone 3.5 hours to watch me. And I had some advantages. It was gluteraldehyde, not formalin. - Gluteraldehyde outgasses less than formalin, so less vapors - Gluteraldehyde is heavier than formain, so stuck around the floor more (hence the long handled dust pan) - The room was designed for a possible gluteraldehyde spill. It had vents around the baseboards, so it could suck in the fumes. Even with these vents and having ventilation increased to maximum, the fumes inside the room (without the respirator) were high - The floor was a 1 piece vinyl, that went up the baseboard by about 6". The floor was designed for a spill. Not 1' square tiles where the chemical could seep between and under. Or where the wall meets the floor, so the chemical can wick up the wall. - Endoscopy was closed for the night. - I had a respirator fit tested for me, the right filters, and lots of experience cleaning up spills (That's why I thought I could handle this spill. But it ended up being right at the edge of my comfort level.) I would not have tried to clean up 5 gallons for formalin in a lab - too much outgassing, ventilation is in the ceiling (going past the nose), formalin getting between tiles and being absorbed into the walls. I definitely would have called in the outside company if it had been a 5 gallon formalin spill in the lab. Now imagine regular lab employees trying to clean up a 5 gallon spill, without a respirator, without a lot of training, without a lot of experience, in a lab setting not designed for spills, with the pressure to hurry up so people could get back to work and make slides. That's what we need to be thinking about, when setting the limits that people can clean up. The safety of the regular employees. (End of editorial.) Peggy A. Wenk, HTL(ASCP)SLS Safety Officer, Anatomic Pathology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, November 05, 2009 3:22 PM To: histonet Subject: [Histonet] lab spill clean-up I am looking for quantities of various lab chemicals that are considered safe for lab personnel to clean up.? Are there set guidelines for when you should call for help? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Nov 6 04:27:59 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Nov 6 04:28:11 2009 Subject: [Histonet] 2010 NSH Teleconferences Message-ID: <1476FEDD2C274B3B963085BBAC9B3DF6@HPPav2> Just to let people know, the 2010 NSH Teleconference information is available on the web http://www.nsh.org/organizations.php3?action=printContentTypeHome&orgid=111& typeID=1380 If that doesn't work, www.nsh.org On left, highlight "Meetings" Click on "NSH Events" Click on "2010 Teleconferences" Click on "Sessions" across the top *NEW* There are 12 sessions, 1 each month. (In the past, we have not had a teleconference the month of the NSH Symposium.) So if you have people who need 12 hours Continuing Education (CE) to maintain their certification, you can get it through NSH teleconferences. Usually 4th Wednesday of month, 1-2 pm Eastern time. If ordered by Jan. 26, 2010, all 12 teleconferences are $1200, or $100 each, compared with $1500 ($125 each). Savings of $300. And that's 1 hour CE for each person in your lab, for $100. Of course, can just order a few at $125 each. If not everyone in your lab can attend the session that day (have to stay in lab and work, on vacation/medical leave, hire someone next month, overworked that day and noone could attend, etc.), about 1-2 months after the teleconference, your lab will receive a CD with the PowerPoint, additional handouts, speaker's voice for their presentation, and a 4 question test. At any time in the next 2 years, everyone in your lab can listen to the presentation, follow the PowerPoint/handouts, take/submit the test, and earn 1 hour CE. (Guess I should have said this was an "ad". Disclaimer - I'm the NSH Teleconference Coordinator, but I don't get any money for doing this job or for getting labs to sign up for teleconferences.) Peggy A. Wenk, HTL(ASCP)SLS NSH Teleconference Coordinator From lpwenk <@t> sbcglobal.net Fri Nov 6 04:39:39 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Nov 6 04:39:49 2009 Subject: [Histonet] November 2009 Muscle NSH Teleconfernce Message-ID: <881FBD51A68449168EB2CE0178E1EB7B@HPPav2> While I'm on the topic of NSH Teleconferences, I'd like to push the next one, Nov. 18, 2009. Muscle Cases. (Warning - advertising NSH teleconferences) This year, all the NSH teleconferences are over 100 participating sites, except this one. It's at 96 sites. I'd really like all to be over 100, as that would be the first time all NSH teleconferences are over 100. It was only about 4 years ago that we got our first teleconference over 100 sites, so this is great news. And this year, we had one teleconference that was over 200, for the first time ever. (So, yeah, this is a personal goal for me.) Dr. Jon Wilson is presenting muscle enzyme and IHC. Showing normal and abnormal (disease) cases. He's presented at NSH before on muscle biopsies, and is a great speaker. The photos are incredible, too. So in additional to my personal goal of getting over 100 sites, it's going to be a great teleconference - seeing what normal and diseased muscle biopsies look like, and why the various enzyme and IHC stains are being used. If interested, go https://www.nshonline.org/eweb/Dynamicpage.aspx?Site=nsh&WebKey=8a7e3247-78d a-486e-8a68-2ebcaf0bc23a&RegPath=EventRegFees&REg_evt_key=ac5fceb0-a554-4d37 -8567-6c0555b5eef2 If that doesn't work, go to www.nsh.org On left click on Meetings Click on NSH Events Click on 2009 Teleconferences There's still time to sign up. Peggy A. Wenk, HTL(ASCP)SLS NSH Teleconference Coordinator (volunteer position, so I get no money from people signing up. Just the satisfacation of seeing people getting education.) From carrolpb <@t> umdnj.edu Fri Nov 6 08:20:40 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Nov 6 08:20:56 2009 Subject: [Histonet] cryopreservation of rodent nerves for frozen sections In-Reply-To: References: Message-ID: <4AF430B8.90304@umdnj.edu> > do people generally use table sugar sure... it works :) laurie@conxis.com wrote: > Hello everyone, Happy Thursday. > > I am working on a project where the investigator is doing immunostaining on thoracic cranial nerves in rat on frozen sections. The initial sections were too riddled with ice artifact to be useable so we are trying again and I am doing sucrose cryopreservation on the nerves, with reduced timing because of sample size. My question is when the sucrose solution is prepped is molecular grade sucrose necessary for this or do people generally use table sugar ( I know... gasp ). > > Just wondering... > > Thank you! > Laurie > > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dmccaig <@t> ckha.on.ca Fri Nov 6 09:29:11 2009 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Nov 6 09:29:17 2009 Subject: [Histonet] Barrett's esophagus Message-ID: We are having problems with our Alcian Blue-Metanil yellow stain that we do routinely on esophagus biopsies. What other stains are being used to demonstrate Barrett's in lieu of this? Diana From lblazek <@t> digestivespecialists.com Fri Nov 6 09:47:59 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Nov 6 09:43:03 2009 Subject: [Histonet] RE: Barrett's esophagus In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390987A86A2C@IBMB7Exchange.digestivespecialists.com> We do Alcian Blue/PAS. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, November 06, 2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Barrett's esophagus We are having problems with our Alcian Blue-Metanil yellow stain that we do routinely on esophagus biopsies. What other stains are being used to demonstrate Barrett's in lieu of this? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Fri Nov 6 09:43:11 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Nov 6 09:43:16 2009 Subject: [Histonet] RE: Barrett's esophagus In-Reply-To: Message-ID: We do an Alcian Blue/PAS stain for Barrett's esophagus. Our paths love it! Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From leswes <@t> shaw.ca Fri Nov 6 09:44:57 2009 From: leswes <@t> shaw.ca (Lesley Weston) Date: Fri Nov 6 09:45:14 2009 Subject: [Histonet] Cutting paraffin sections in a warm room In-Reply-To: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> References: <858249120911051010w338b9a1cu67bb26aabc53957f@mail.gmail.com> Message-ID: <0DD568B0-E4BC-44BA-B25C-4CCA72E5F503@shaw.ca> I used to work in a building with no air conditioning. In Summer, I used to rig up a wide-mouthed plastic funnel over the microtome and place a piece of solid CO2 in the funnel. The cold air flowed down over the block and made cutting possible. Crude but effective. Lesley Weston. On 5-Nov-09, at 10:10 AM, Adam . wrote: > Hi all, > > I've just recently started cutting paraffin sections (of bones). A > few weeks > ago, the facilities people decided that it was fall and now the lab is > significantly warmer than it used to be, and my paraffin is falling > apart > and sticking to everything. Apparently, we can't control the > temperature > through a thermostat at all, and the microtome is inside the lab. I > was > wondering if anyone had any ideas on how to section in a warm room. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Fri Nov 6 09:46:30 2009 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Nov 6 09:46:49 2009 Subject: [Histonet] Minneapolis VA Histotech job -Apply now thru Nov. 16 Message-ID: DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN. Full time Histotech. opportunity at Minneapolis VA. BS or BA in Biology. Either HT or HTL required. Prefer 5 yr. exp. IHC experience a plus. Effective interpersonal skills required. Holiday, evenings and weekends off. Excellent bene's. Detail oriented. Responsible for technical and procedural operations of the dept., performing quality control, quality improvement and regulatory compliance tasks. The job is posted on www.usajobs.gov . Applications will be accepted through November 16, 2009. Type in "Histotechnologist" or the vacancy announcement #: pg-10-scm-297501. Please contact me if you have any questions: Sandra Harrison, Histology Supervisor, at Sandra.Harrison3@va.gov. Principals only. No recruiters please. From cbrya <@t> lexclin.com Fri Nov 6 10:15:26 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Nov 6 10:15:30 2009 Subject: [Histonet] thanks Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF17A22BED@EXCHANGESB> Just wanted to thank everyone for all the wonderful suggestions and ideas for our workflow issues I posted yesterday. I appreciate everyone?s timing responding! ? Have a great weekend! Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From brian <@t> prometheushealthcare.com Fri Nov 6 11:16:28 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Fri Nov 6 11:16:33 2009 Subject: [Histonet] New Histology Openings in Westchester, NY Message-ID: <017f01ca5f04$e0b0c6f0$a21254d0$@com> Please let me know if you might be interested in any of the positions listed below. They are located in Westchester county We also offer large referral bonuses if you are able to refer anyone that we are able to place. Thanks in advance for your help. open positions summary HISTOLOGY as of 11/5/09 Grossing Tech 2.00 8pm-4:30am (2) tues-sat Histotech I 3.00 3 AM - 11.30 AM tues-sat 3 AM - 11.30 AM-tues-sat HistoTech 2.00 5 PM - 1.30 AM tues-sat 6PM-2:30PM tues-sat Histotech II-IHC 1.00 Hold FISH 2.00 6 AM - 2.30 (T - S) 8 AM - 4.30 (M-F) Total FTE 10.00 CYTOLOGY POSITIONS HistoTech 1.00 3AM-11:30AM (T-S) Cytoprep tech 2.00 10pm-6:30am (t-s) Total FTE 3.00 TOTAL OPEN POSITIONS = 13.0 Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From jrobertson <@t> pathologysciences.com Fri Nov 6 11:19:37 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Fri Nov 6 11:19:43 2009 Subject: [Histonet] OSCAR antibody In-Reply-To: <4AF34188.6090508@umdnj.edu> References: <4AF34188.6090508@umdnj.edu> Message-ID: <518CD6920AA7154193CBE5977CD880733A8C42@psmgsrv2.PSMG.local> Our Second Cytokeratin Antibody Rocks Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Thursday, November 05, 2009 1:20 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OSCAR antibody osteoclast-associated receptor Maria Katleba wrote: > What do the letters that make up the name OSCAR mean ? There is a debate in my lab over it? :) > > > > Maria Katleba HT(ASCP), MS > Pathology Dept. Mgr. > Queen of the Valley Medical Center > 707-294-9229 cell > 707-252-4411 x3689 > > > > ________________________________ > Notice from St. Joseph Health System: > Please note that the information contained in this message may be privileged and confidential and protected from disclosure. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Mon Nov 9 09:54:12 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Nov 9 09:54:16 2009 Subject: [Histonet] ASCP Question Message-ID: Hello All, I want to know how hard it is, if possible to become HT ASCP certified with a background of just having a Bachelor's Degree in Biology. I heard that if you have a Bachelor's degree in a life science than you are eligible for certification. Is it possible to take the test without going through a histology program and just having a BS in Biology? -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From godsgalnow <@t> aol.com Mon Nov 9 10:18:25 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Nov 9 10:18:57 2009 Subject: [Histonet] ASCP Question In-Reply-To: References: Message-ID: <8CC2F6F662D70AD-AE14-17A75@webmail-d017.sysops.aol.com> lRoute 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; or lRoute 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or Associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. -----Original Message----- From: Alyssa Peterson To: histonet@lists.utsouthwestern.edu Sent: Mon, Nov 9, 2009 10:54 am Subject: [Histonet] ASCP Question Hello All, I want to know how hard it is, if possible to become HT ASCP certified with background of just having a Bachelor's Degree in Biology. I heard that if ou have a Bachelor's degree in a life science than you are eligible for ertification. Is it possible to take the test without going through a istology program and just having a BS in Biology? -- Be sure to visit us on the web* www.alliedsearchpartners.com lyssa Peterson, Director Of Recruitment llied Search Partners :888.388.7571 ext. 101 : 888.388.7572 ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Mon Nov 9 10:34:16 2009 From: alisha <@t> ka-recruiting.com (Alisha Taylor) Date: Mon Nov 9 10:33:26 2009 Subject: [Histonet] Histotechnologist Job in Oklahoma! Message-ID: <1011983025.1257784456349.JavaMail.cfservice@webserver57> Dear Histotechnologists, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a mid-sized nonprofit hospital in southwest Oklahoma. This area is known for its year round recreational activities as it's located near the mountains and a lake. My client is looking for histotech for their 1st shift position. The ideal candidate will have graduated from an accredited Histotechnology school, will be ASCP certified as an HT or HTL, and will have greater than 1 year of experience in a histology lab. My client offers a very competitive hourly rate, full benefits, and possible relocation assistance, if needed. Please email or call me if you have an interest in learning more about this position. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Other Current Histology Opportunities: Histology Supervisor - Georgia (1st shift) Histotechnologist- Georgia (1st shift) Histotechnologist - Southern CA (all shifts) Histology Supervisor- Southern CA (3rd shift) Pathology Manager - Bay Area, CA (1st shift) Histotechnologist - Boston, MA (1st shift) Histotechnologist - Oklahoma Histology Supervisor- Oklahoma (1st shift) Histotechnologist - New York City, NY (3rd shift) Pathology Manager - New York City, NY (1st shift) Histotechnologist - Otsego County, NY (Upstate NY) If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha R. Taylor, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alyssa <@t> alliedsearchpartners.com Mon Nov 9 10:37:42 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Nov 9 10:37:52 2009 Subject: [Histonet] Job In Atlanta, GA with Private Lab Message-ID: Allied Search Partners is currently accepting resumes in Atlanta for qualified Histotechnicians/Histotechnologists. Job Description: Process and make pathology slides and assist with Moh's surgery. Currently the laboratory is sending out about 5,000 pathology skin specimens a year and will be increasing that number over the next year. Histotechnologists/Histotechnicians Shifts: Day Shift Location: Private Lab Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* upon resume submission one of our recruiters will call you for an initial phone interview. No resume will be submitted before an initial phone interview. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From Bauer.Karen <@t> mayo.edu Mon Nov 9 11:51:33 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Mon Nov 9 11:51:37 2009 Subject: [Histonet] Microm vs Leica Message-ID: <53FC421CC200C5429929EDE6C3676F306E15F9@msgebe34> Hello, We are starting up a new Mohs lab and there have been discussions on what brand of cryostat to purchase. The new doc would like us to get Microm cryostats, but I would like to stick with the Leica, since we us them in the Histology lab and know and trust them. They have been workhorses for us and very reliable. What is the opinion of other Mohs techs out there? Any major differences? Is one really superior to the other? Any comments on this matter is appreciated. Thank you, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu From Beatrice.Debrosse-Serra <@t> pfizer.com Mon Nov 9 12:16:10 2009 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Mon Nov 9 12:16:28 2009 Subject: [Histonet] Microm vs Leica In-Reply-To: <53FC421CC200C5429929EDE6C3676F306E15F9@msgebe34> References: <53FC421CC200C5429929EDE6C3676F306E15F9@msgebe34> Message-ID: We demoed them both and all of us liked the Leica cryostat better. But then again, we are not a Mohs lab. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Monday, November 09, 2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microm vs Leica Hello, We are starting up a new Mohs lab and there have been discussions on what brand of cryostat to purchase. The new doc would like us to get Microm cryostats, but I would like to stick with the Leica, since we us them in the Histology lab and know and trust them. They have been workhorses for us and very reliable. What is the opinion of other Mohs techs out there? Any major differences? Is one really superior to the other? Any comments on this matter is appreciated. Thank you, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Mon Nov 9 13:51:51 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Nov 9 13:52:03 2009 Subject: [Histonet] Va mini-Learn and Earn Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A77A2@wlmmsx01.nemours.org> Thanks you to the attendees of last Saturdays, very successful VA mini-LEARN and EARN in VA Beach. For those who attended, and wish color copies of the handouts... send a request for the site posting: cbarone@nemours.org Congratulations to Virginia Society ... for your efforts in re-activating your state society and to the support states of Region II, DE, NJ, MD and PA. . For those who wish to join the VA Society, contact Thompson, Sophie K. [sxthomps@odu.edu] or Roger Roark [rroark@portercreekinstruments.com] - for more information on member registration. We hope everyone will join their state society, the NSH and also be "histo-netters", too! Histologists in support of all Histologist! March 10, 2010.....It's coming!!!...Go to: www.nsh.org....... From amosbrooks <@t> gmail.com Mon Nov 9 14:57:40 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Nov 9 14:57:48 2009 Subject: [Histonet] Fc Receptors Message-ID: <582736990911091257g1921ca7dr5315622e223f2fac@mail.gmail.com> Hi, I have a question about Fc Receptors. I have samples that I have IHC labelled with a few antibodies. Some were totally fine while others had a lot of background which was eliminated when repeated with a Fc Receptor blocker (Innovex). So my question is this: Is there a general rule of thumb about when such a treatment would be useful? Using it all the time would work, but aside from being unnecessary sometimes, it would prove fairly pricey too. Any Suggestions? Amos -- Sent from my mobile device From amosbrooks <@t> gmail.com Mon Nov 9 14:59:12 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Nov 9 14:59:18 2009 Subject: [Histonet] Fc blocker again Message-ID: <582736990911091259x66caa156la56ecb8eec893e7b@mail.gmail.com> Oh, I should mention I'm using mouse tissue. Thanks, Amos -- Sent from my mobile device From mtrhill <@t> msn.com Mon Nov 9 15:08:25 2009 From: mtrhill <@t> msn.com (marty hill) Date: Mon Nov 9 15:08:31 2009 Subject: [Histonet] job Message-ID: We are looking for a certified histo tech. Job is in Fresno, Ca. at Kaiser Hospital. Look forward to hearing from you. _________________________________________________________________ Bing brings you maps, menus, and reviews organized in one place. http://www.bing.com/search?q=restaurants&form=MFESRP&publ=WLHMTAG&crea=TEXT_MFESRP_Local_MapsMenu_Resturants_1x1 From Bauer.Karen <@t> mayo.edu Mon Nov 9 15:18:26 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Mon Nov 9 15:18:30 2009 Subject: [Histonet] IgG4 antibody Message-ID: <53FC421CC200C5429929EDE6C3676F306E1605@msgebe34> Hello, I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP stainer. Is anyone out there using this antibody on their XT or Ultra? If so, could you please share with me the vendor you go through and what you are using for a protocol? Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu From cbarone <@t> NEMOURS.ORG Mon Nov 9 15:40:04 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Nov 9 15:40:27 2009 Subject: [Histonet] Looking for VA TECH - EDU Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A77AC@wlmmsx01.nemours.org> Virginia Histo-netters........I am trying to re-locate the person from VA Tech.edu...who e-mailed me and was interested in being part of the new VA Histology Society?... or anyone at VA Tech... No matter where you are, anyone, ...if you would be interested in paticipating. contact me at: cbarone@nemours.org . The new society will have chapters, to lesson travel across such a wide state.....we are looking for someone interested in being involved with the western Virginia area. Virginia, you asked for it to happen...and it is happening!!!!! A new and improved Histology Society for the State of Virginia.....Hey, D.C. area....we'd like you to join us, too !!!!! Contact: sxthomps@odu.edu regarding membership and/or participation in the society...all you need is a telephone and e-mail, to do it!!!! From Laura.Miller <@t> leica-microsystems.com Mon Nov 9 16:01:57 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Mon Nov 9 16:02:03 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 11/09/2009 and will not return until 11/11/2009. I will be attending the Leica educational symposia in Cincinnati on Monday and Tuesday. I will be back in the office on Wednesday, Novemebr 11th. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mpence <@t> grhs.net Mon Nov 9 16:11:10 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Nov 9 16:11:14 2009 Subject: [Histonet] Control Tissue for IHC H-pylori Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3C75@is-e2k3.grhs.net> What is anyone using for their control tissue for IHC H-pylori. We have few and far between gastric resection here and even if we did more, it is a hit and miss for the organism. We have a policy here to not used labeled patient tissue blocks as controls. Thanks, Mike From pruegg <@t> ihctech.net Mon Nov 9 16:12:47 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Nov 9 16:13:25 2009 Subject: SPAM-LOW: [Histonet] IgG4 antibody In-Reply-To: <53FC421CC200C5429929EDE6C3676F306E1605@msgebe34> References: <53FC421CC200C5429929EDE6C3676F306E1605@msgebe34> Message-ID: <24F0D0CB2AB64F94999E335047A56992@Patsyoffice> I am not using this on a Ventana but a really good place to get reagents like that is southernbiotech.com I see they have 2 IgG4(Fc),human (mouse anti-) and IgG4(pFc),human (mouse anti-) Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Monday, November 09, 2009 2:18 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] IgG4 antibody Hello, I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP stainer. Is anyone out there using this antibody on their XT or Ultra? If so, could you please share with me the vendor you go through and what you are using for a protocol? Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Nov 9 17:13:14 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Nov 9 17:13:40 2009 Subject: SPAM-LOW: [Histonet] Fc Receptors In-Reply-To: <582736990911091257g1921ca7dr5315622e223f2fac@mail.gmail.com> References: <582736990911091257g1921ca7dr5315622e223f2fac@mail.gmail.com> Message-ID: <636A7C6696EA4DAC86D7D3D9C39ABE98@Patsyoffice> Amos, I use fc blocker for all mouse on mouse staining, staining on sheep tissue when my detection is made in a goat as many of them are, and in cases like you just showed where I get non specific BG staining, I don't know if you can make up a rule but these are my criteria. You are right it is just too expensive to use all the time. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, November 09, 2009 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Fc Receptors Hi, I have a question about Fc Receptors. I have samples that I have IHC labelled with a few antibodies. Some were totally fine while others had a lot of background which was eliminated when repeated with a Fc Receptor blocker (Innovex). So my question is this: Is there a general rule of thumb about when such a treatment would be useful? Using it all the time would work, but aside from being unnecessary sometimes, it would prove fairly pricey too. Any Suggestions? Amos -- Sent from my mobile device _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kwuny <@t> email.cs.nsw.gov.au Mon Nov 9 20:17:59 2009 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Mon Nov 9 20:18:10 2009 Subject: [Histonet] IgG4 antibody In-Reply-To: <53FC421CC200C5429929EDE6C3676F306E1605@msgebe34> References: <53FC421CC200C5429929EDE6C3676F306E1605@msgebe34> Message-ID: <0D2DAED8426F46EAAB3B96ECFD8C4A62@cs.nsw.gov.au> Dear Karen, We are using Invitrogen's monoclonal Anti-human IgG4 (Cat No. 05-3800) with a working dilution around 1/700. Although we are using BondMax stainer with 30 min, Epitope Retrieval solution 2 (alkaline) pretreatment from Leica, Ventana would give a good staining as well. Young Sun Young Kwun Senior Hospital Scientist Immunohistochemistry Department of Anatomical Pathology Concord Hospital, Concord NSW Australia 61-2-97676075 (tel) 61-2-9767-8427 (fax) 0408868288 (mob) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, 10 November 2009 8:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG4 antibody Hello, I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP stainer. Is anyone out there using this antibody on their XT or Ultra? If so, could you please share with me the vendor you go through and what you are using for a protocol? Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From jshelley <@t> burnham.org Tue Nov 10 08:25:13 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Tue Nov 10 08:25:19 2009 Subject: [Histonet] Tissue falling off slides Message-ID: Good morning histonetters!! I received a project from an outside source to process and embed mouse hearts for this group. From what I could gather the specimens had varying times in 4% paraformaldehyde from 24-48 hours all the way up to 4 months. Once processed and embedded the specimens where returned to the group to cut. They are using superfrost plus slides from Fisherbrand and from what I could see the sections were coming off fine. The only thing I did notice is that when they put the slide in the water bath the tissue seemed to be repelled which from experience means that too much coating on slide and a hydrophobic environment is create. I asked them to look for slides from a different lot and cut additional sections and from those sections I asked them to air-dry only and others I asked for them to place in oven for 30-45 mins. at 56 to 60 degrees or until they did not see any more water droplets. They said the sections that they placed in the oven stayed on a little longer in the deparaffinizing steps but still fell off. I know that it could be processing also but the specimens looked great when I embedded them. Any suggestions will be great, sometimes when your right on top of it you lose sight of other possibilities. John From JWeems <@t> sjha.org Tue Nov 10 08:35:14 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Nov 10 08:35:48 2009 Subject: [Histonet] Tissue falling off slides In-Reply-To: References: Message-ID: Put some alcohol in the water bath - it will reduce the surface tension. Maybe that will help. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Tuesday, November 10, 2009 09:25 To: histonet@lists.utsouthwestern.edu Cc: Betsy Woessner; Kathryn Gizzi Subject: [Histonet] Tissue falling off slides Good morning histonetters!! I received a project from an outside source to process and embed mouse hearts for this group. From what I could gather the specimens had varying times in 4% paraformaldehyde from 24-48 hours all the way up to 4 months. Once processed and embedded the specimens where returned to the group to cut. They are using superfrost plus slides from Fisherbrand and from what I could see the sections were coming off fine. The only thing I did notice is that when they put the slide in the water bath the tissue seemed to be repelled which from experience means that too much coating on slide and a hydrophobic environment is create. I asked them to look for slides from a different lot and cut additional sections and from those sections I asked them to air-dry only and others I asked for them to place in oven for 30-45 mins. at 56 to 60 degrees or until they did not see any more water droplets. They said the sections that they placed in the oven stayed on a little longer in the deparaffinizing steps but still fell off. I know that it could be processing also but the specimens looked great when I embedded them. Any suggestions will be great, sometimes when your right on top of it you lose sight of other possibilities. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From julia.m.hough <@t> hotmail.co.uk Tue Nov 10 08:37:02 2009 From: julia.m.hough <@t> hotmail.co.uk (julia hough) Date: Tue Nov 10 08:37:06 2009 Subject: [Histonet] CD138 Message-ID: Dear histonetters. I am trying to perform several new IHC stains on mouse tibia. However, I am having trouble finding literature on some of them. I would like an antibody for CD138 that work on mouse tissue/bone that are preferably conjugated to a fluorchrome. If anyone has any protocols or relevant references that'd be lovely. Thanks Julia _________________________________________________________________ Have more than one Hotmail account? Link them together to easily access both http://clk.atdmt.com/UKM/go/186394591/direct/01/ From relia1 <@t> earthlink.net Tue Nov 10 09:39:36 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Nov 10 09:39:40 2009 Subject: [Histonet] PA needed in Charlotte, NC Can You Help? Message-ID: Hello Histonetters! My name is Pam Barker and my company is RELIA Solutions. RELIA Solutions is a nationwide recruiting firm specializing in the permanent placement of anatomic pathology professionals. My services are free of charge to job seekers and all of my fees are paid by the companies I represent. I am contacting you in hopes that you might be able to help me. The help that I need is that I am working with one of my best clients located in Charlotte, NC that is in need of a PA. My client is a strong established and growing lab and they offer excellent compensation, benefits and relocation assistance. This is a permanent full time day shift position. My client needs someone who is a graduate of an NACCLES accredited school. They are interested in speaking with recent graduates as well as experienced PAs. If you or someone you know might be interested in hearing more about this opportunity please contact me at relia1@earthlink.net or toll free at 866-607-3542. I am available to talk at your convenience including after hours. Thank you for taking the time to read this e-mail. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia From jackdodo <@t> msn.com Tue Nov 10 09:52:05 2009 From: jackdodo <@t> msn.com (Jack Dodo) Date: Tue Nov 10 09:52:08 2009 Subject: [Histonet] Productivity for Microtomy and Embedding Message-ID: I hope I am not opening a can of worms here, but I would like to see what the consensus is for productivity standards on embedding and cutting. Just keep in mind that I am mainly derm specimens. Thanks All! _________________________________________________________________ Windows 7: Unclutter your desktop. http://go.microsoft.com/?linkid=9690331&ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen:112009 From rjbuesa <@t> yahoo.com Tue Nov 10 10:03:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 10 10:03:20 2009 Subject: [Histonet] Productivity for Microtomy and Embedding In-Reply-To: Message-ID: <473191.29329.qm@web65710.mail.ac4.yahoo.com> Jack: First of all, you cannot ask for a general consensus and immediately add a caveat for dermatology cases. The average for 241 histolabs is: 50 blocks/hour embedding and 24 blocks/hour cutting. Under separate cover I am sending you other data. Ren? J. --- On Tue, 11/10/09, Jack Dodo wrote: From: Jack Dodo Subject: [Histonet] Productivity for Microtomy and Embedding To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 10, 2009, 10:52 AM I hope I am not opening a can of worms here, but I would like to see what the consensus is for productivity standards on embedding and cutting. Just keep in mind that I am mainly derm specimens. Thanks All! ??? ???????? ?????? ??? ? _________________________________________________________________ Windows 7: Unclutter your desktop. http://go.microsoft.com/?linkid=9690331&ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen:112009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue Nov 10 10:30:53 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Nov 10 10:31:10 2009 Subject: [Histonet] Productivity for Microtomy and Embedding In-Reply-To: References: Message-ID: <8CC303A4E41ED31-6954-B6E2@webmail-d032.sysops.aol.com> The productivity standards that I expect here for derms are Embedding 40 /hr Cutting 25/hr (3 levels required each block for punches and shaves) -----Original Message----- From: Jack Dodo To: histonet@lists.utsouthwestern.edu Sent: Tue, Nov 10, 2009 10:52 am Subject: [Histonet] Productivity for Microtomy and Embedding hope I am not opening a can of worms here, but I would like to see what the onsensus is for productivity standards on embedding and cutting. Just keep in ind that I am mainly derm specimens. Thanks All! ________________________________________________________________ indows 7: Unclutter your desktop. ttp://go.microsoft.com/?linkid=9690331&ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen:112009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcumming <@t> ucalgary.ca Tue Nov 10 10:47:43 2009 From: mcumming <@t> ucalgary.ca (Murray Cumming) Date: Tue Nov 10 10:48:08 2009 Subject: [Histonet] large eye specimens Message-ID: <008801ca6225$85cdced0$91696c70$@ca> Hi Y'all We are looking at cutting animal eyeballs for our vets. I was wondering if anyone could direct me to resources for processing super large eyes - like horses & cows. Thank you, Murray Cumming Histotechnologist Pathology Lab Clinical Skills Bldg. 403-210-7372 From stamptrain <@t> yahoo.com Tue Nov 10 11:28:01 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Tue Nov 10 11:28:06 2009 Subject: [Histonet] large eye specimens In-Reply-To: <008801ca6225$85cdced0$91696c70$@ca> References: <008801ca6225$85cdced0$91696c70$@ca> Message-ID: <750265.81321.qm@web55805.mail.re3.yahoo.com> Search the histonet archives on this subject. I know it has been discussed a number of times. Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: Murray Cumming To: histonet@lists.utsouthwestern.edu Sent: Tue, November 10, 2009 11:47:43 AM Subject: [Histonet] large eye specimens Hi Y'all We are looking at cutting animal eyeballs for our vets. I was wondering if anyone could direct me to resources for processing super large eyes - like horses & cows. Thank you, Murray Cumming Histotechnologist Pathology Lab Clinical Skills Bldg. 403-210-7372 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Tue Nov 10 11:34:05 2009 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Tue Nov 10 11:34:21 2009 Subject: [Histonet] large eye specimens In-Reply-To: <008801ca6225$85cdced0$91696c70$@ca> References: <008801ca6225$85cdced0$91696c70$@ca> Message-ID: It's in the Animal Processing Manual from N.S.H. Ruth Yaskovich N.I.H. -----Original Message----- From: Murray Cumming [mailto:mcumming@ucalgary.ca] Sent: Tuesday, November 10, 2009 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] large eye specimens Hi Y'all We are looking at cutting animal eyeballs for our vets. I was wondering if anyone could direct me to resources for processing super large eyes - like horses & cows. Thank you, Murray Cumming Histotechnologist Pathology Lab Clinical Skills Bldg. 403-210-7372 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbrereton <@t> lmhosp.org Tue Nov 10 12:09:28 2009 From: mbrereton <@t> lmhosp.org (Brereton, Martha) Date: Tue Nov 10 12:09:33 2009 Subject: [Histonet] Productivity for Microtomy and Embedding In-Reply-To: <473191.29329.qm@web65710.mail.ac4.yahoo.com> References: <473191.29329.qm@web65710.mail.ac4.yahoo.com> Message-ID: Renee, Where are these 241 histolabs and what criteria are they using? Can you forward that info to me also and anything else that may be helpful regarding this. Thanks. My personal email is mmbhw@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 10, 2009 11:03 AM To: histonet@lists.utsouthwestern.edu; Jack Dodo Subject: Re: [Histonet] Productivity for Microtomy and Embedding Jack: First of all, you cannot ask for a general consensus and immediately add a caveat for dermatology cases. The average for 241 histolabs is: 50 blocks/hour embedding and 24 blocks/hour cutting. Under separate cover I am sending you other data. Ren? J. --- On Tue, 11/10/09, Jack Dodo wrote: From: Jack Dodo Subject: [Histonet] Productivity for Microtomy and Embedding To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 10, 2009, 10:52 AM I hope I am not opening a can of worms here, but I would like to see what the consensus is for productivity standards on embedding and cutting. Just keep in mind that I am mainly derm specimens. Thanks All! ??? ???????? ?????? ??? ? _________________________________________________________________ Windows 7: Unclutter your desktop. http://go.microsoft.com/?linkid=9690331&ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen:112009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From mbrereton <@t> lmhosp.org Tue Nov 10 12:14:38 2009 From: mbrereton <@t> lmhosp.org (Brereton, Martha) Date: Tue Nov 10 12:14:46 2009 Subject: [Histonet] Competency and training forms Message-ID: Hello all, I am starting in a new position and the competency and training forms are outdated. I have created my own, however, I would like to know if anyone out there is willing to share what they currently use. I have enclosed my personal email if you do not want to send it over the histonet. Thanks mmbhw@aol.com This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From rsrichmond <@t> gmail.com Tue Nov 10 12:32:45 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Nov 10 12:32:49 2009 Subject: [Histonet] Re: Control Tissue for IHC H-pylori Message-ID: Mike Pence asks about control tissue for immunohistochemical staining of Helicobacter. You really do need a positive control for every run of this stain, and it's one stain where the negative control is also actually needed, on account of background staining. Gastrectomy specimens positive for Helicobacter are rather rare now. I think that you must have some way of detaching the patient's identification from your control material. This is a situation where you may actually need to communicate with your pathologist. Parenthetically, Helicobacter heilmannii, which I have seen once in my life, also reacts with Helicobacter antibodies used for immunostaining. Bob Richmond Samurai Pathologist Knoxville TN From cmmathis1 <@t> bellsouth.net Tue Nov 10 13:43:56 2009 From: cmmathis1 <@t> bellsouth.net (RICKY MATHIS) Date: Tue Nov 10 13:45:05 2009 Subject: [Histonet] List of Resins and plastic embedding mediums In-Reply-To: <0D2DAED8426F46EAAB3B96ECFD8C4A62@cs.nsw.gov.au> References: <53FC421CC200C5429929EDE6C3676F306E1605@msgebe34> <0D2DAED8426F46EAAB3B96ECFD8C4A62@cs.nsw.gov.au> Message-ID: <865359.34237.qm@web180601.mail.sp1.yahoo.com> Hello all histo-netters, I want to know if there is a list of resins and plastic embedding mediums that would also give there properties, stainability, etc.? I am a novice in this area and have received many requests lately for embedding without heat and/or without xylene (or other solvents).? My 33 years have all been with paraffin and frozen sections.? Any information would be greatly appreciated. Cathy ________________________________ From: Young Kwun To: "Bauer, Karen L." Cc: Histonet Sent: Mon, November 9, 2009 9:17:59 PM Subject: RE: [Histonet] IgG4 antibody Dear Karen, We are using Invitrogen's monoclonal Anti-human IgG4 (Cat No. 05-3800) with a working dilution around 1/700. Although we are using BondMax stainer with 30 min, Epitope Retrieval solution 2 (alkaline) pretreatment from Leica, Ventana would give a good staining as well. Young Sun Young Kwun Senior Hospital Scientist Immunohistochemistry Department of Anatomical Pathology Concord Hospital, Concord NSW Australia 61-2-97676075 (tel) 61-2-9767-8427 (fax) 0408868288 (mob) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, 10 November 2009 8:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG4 antibody Hello, I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP stainer.? Is anyone out there using this antibody on their XT or Ultra? If so, could you please share with me the vendor you go through and what you are using for a protocol? Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Tue Nov 10 15:41:07 2009 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Tue Nov 10 15:41:14 2009 Subject: [Histonet] Helicobacter pylori IHC stain Message-ID: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter pylori antibody from, as well as any information including antibody clone, dilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From marktarango <@t> gmail.com Tue Nov 10 16:52:39 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Nov 10 16:52:43 2009 Subject: [Histonet] Re: [IHCRG] Helicobacter pylori IHC stain In-Reply-To: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> Message-ID: <5b6eb13e0911101452u452d45a4med0ae2032b62f38@mail.gmail.com> We use the polyclonal from cellmarque. 1:200 on the Ventana XT. We use CC1 mild and then do protease 2 for 8 minutes to get a nice clean stain. Mark Tarango On Tue, Nov 10, 2009 at 1:41 PM, Taylor, Jean wrote: > Hi everyone, > > > > Just trying to get an idea of where labs are purchasing their helicobacter > pylori antibody from, as well as any information including antibody clone, > dilution, detection, etc. I currently run the DAKO platform. > > > > Thank you! > > > > Jean Taylor, HT(ASCP)QIHC > > IHC Tech > > Meriter Health Services > > Madison, WI > From Maria.Katleba <@t> stjoe.org Wed Nov 11 05:32:45 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Nov 11 05:33:19 2009 Subject: [Histonet] RE: Helicobacter pylori IHC stain In-Reply-To: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> Message-ID: Ventana...is a pre-dilute item # 760-2645 :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, November 10, 2009 1:41 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Helicobacter pylori IHC stain Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter pylori antibody from, as well as any information including antibody clone, dilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From lblazek <@t> digestivespecialists.com Wed Nov 11 06:26:46 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Nov 11 06:21:43 2009 Subject: [Histonet] RE: Helicobacter pylori IHC stain In-Reply-To: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390989DF0A05@IBMB7Exchange.digestivespecialists.com> Jean, Try the new H-Pylori that BioCare has. It's beautiful! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, November 10, 2009 4:41 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Helicobacter pylori IHC stain Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter pylori antibody from, as well as any information including antibody clone, dilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Wed Nov 11 06:49:56 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Nov 11 06:50:29 2009 Subject: [Histonet] RE: Helicobacter pylori IHC stain In-Reply-To: <6F33D8418806044682A391273399860F01924384@s-irv-ex301.PathologyPartners.intranet> References: <6F33D8418806044682A391273399860F01924384@s-irv-ex301.PathologyPartners.intranet> Message-ID: Haley is right! That's why we chose it as well! It will not inadvertently stain 'other' bugs. ________________________________ From: Hale, Meredith [mailto:mhale@carisdx.com] Sent: Wednesday, November 11, 2009 4:11 AM To: Maria Katleba; jtaylor@meriter.com; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Helicobacter pylori IHC stain We use the Cell Marque / Ventana predilute as well and its the cleanest HP antibody we have found . Very crisp and beautiful ! Meredith Hale HT (ASCP)cm Caris Diagnostics ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: Taylor, Jean ; 'ihcrg@googlegroups.com' ; 'histonet@lists.utsouthwestern.edu' Sent: Wed Nov 11 05:32:45 2009 Subject: [Histonet] RE: Helicobacter pylori IHC stain Ventana...is a pre-dilute item # 760-2645 :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, November 10, 2009 1:41 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Helicobacter pylori IHC stain Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter pylori antibody from, as well as any information including antibody clone, dilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Wed Nov 11 06:50:41 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Nov 11 06:51:12 2009 Subject: [Histonet] RE: Helicobacter pylori IHC stain In-Reply-To: <6F33D8418806044682A391273399860F01924384@s-irv-ex301.PathologyPartners.intranet> References: <6F33D8418806044682A391273399860F01924384@s-irv-ex301.PathologyPartners.intranet> Message-ID: Whoops.. I mean Hale... sorry :) its 5am and I have been here all night... ________________________________ From: Hale, Meredith [mailto:mhale@carisdx.com] Sent: Wednesday, November 11, 2009 4:11 AM To: Maria Katleba; jtaylor@meriter.com; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Helicobacter pylori IHC stain We use the Cell Marque / Ventana predilute as well and its the cleanest HP antibody we have found . Very crisp and beautiful ! Meredith Hale HT (ASCP)cm Caris Diagnostics ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: Taylor, Jean ; 'ihcrg@googlegroups.com' ; 'histonet@lists.utsouthwestern.edu' Sent: Wed Nov 11 05:32:45 2009 Subject: [Histonet] RE: Helicobacter pylori IHC stain Ventana...is a pre-dilute item # 760-2645 :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, November 10, 2009 1:41 PM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Helicobacter pylori IHC stain Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter pylori antibody from, as well as any information including antibody clone, dilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Nov 11 08:53:38 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Nov 11 08:53:43 2009 Subject: [Histonet] Ferritin and MMP9 Antibody Message-ID: <16903.36766.qm@web50306.mail.re2.yahoo.com> Hi All, What is everyone using to stain for ferritin and MMP9 on FFPE human tissue? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From plucas <@t> biopath.org Wed Nov 11 09:23:13 2009 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Nov 11 09:23:17 2009 Subject: [Histonet] Sakura Prisma Stainer Message-ID: <000701ca62e2$e28490b0$0f01a8c0@biopath.local> Hello all, We are checking out the prisma stainer from Sakura. Would anyone mind sending me a private email to let me know your experiences with this stainer? Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group From b-frederick <@t> northwestern.edu Wed Nov 11 09:39:25 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Nov 11 09:39:31 2009 Subject: [Histonet] Ferritin and MMP9 Antibody In-Reply-To: <16903.36766.qm@web50306.mail.re2.yahoo.com> Message-ID: We use MMP-9 from LabVision. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, November 11, 2009 8:54 AM To: Histonet Subject: [Histonet] Ferritin and MMP9 Antibody Hi All, What is everyone using to stain for ferritin and MMP9 on FFPE human tissue? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deliadfam <@t> yahoo.com Wed Nov 11 10:12:19 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Wed Nov 11 10:12:24 2009 Subject: [Histonet] Hematoxylin Message-ID: <141574.55383.qm@web63103.mail.re1.yahoo.com> I need some pointers on how to intensify the hematoxylin on the H and E stain. Is there a way to do that? I currently use Richard Allan hematoxylin 7211. The H is weak. All reagents were changed out last night. Any of your expertise would be of great help. Thank you guys in advance. ? Delia From contact <@t> excaliburpathology.com Wed Nov 11 10:20:38 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Nov 11 10:20:42 2009 Subject: [Histonet] Hematoxylin In-Reply-To: <141574.55383.qm@web63103.mail.re1.yahoo.com> References: <141574.55383.qm@web63103.mail.re1.yahoo.com> Message-ID: <170383.72089.qm@web1102.biz.mail.sk1.yahoo.com> hi, first make sure the reagents were replaced in the correct positions if you are using an automatic stainer. Increase the time in hemat. I use Gill III for 10 minutes, water wash, acid alcohol- 4 dips, water wash, 1% ammonia water-1min, water wash. Paula ________________________________ From: DELIA GARCIA To: histonet@lists.utsouthwestern.edu Sent: Wed, November 11, 2009 10:12:19 AM Subject: [Histonet] Hematoxylin I need some pointers on how to intensify the hematoxylin on the H and E stain. Is there a way to do that? I currently use Richard Allan hematoxylin 7211. The H is weak. All reagents were changed out last night. Any of your expertise would be of great help. Thank you guys in advance. ? Delia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Wed Nov 11 10:30:24 2009 From: histonet.nospam <@t> vneubert.com (histonet.nospam@vneubert.com) Date: Wed Nov 11 10:30:29 2009 Subject: [Histonet] Re: [ Histonet ] Hematoxylin In-Reply-To: <141574.55383.qm@web63103.mail.re1.yahoo.com> Message-ID: <20091111163024.F2F32C289A03@dd15630.kasserver.com> Gill II (ThermoFisher) 5 min, 3x1 min A. Dest. That's it :) > I need some pointers on how to intensify the hematoxylin on the H and E stain. Is there a way to do that? I currently use Richard Allan hematoxylin 7211. The H is weak. All reagents were changed out last night. Any of your expertise would be of great help. Thank you guys in advance. > > Delia > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Wed Nov 11 12:22:38 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Nov 11 12:22:52 2009 Subject: [Histonet] Re: Previous Technician of mine Looking for a position in Wilmington, NC Message-ID: Hello All, Posting this for an old traveler/technician of mine: She is looking for a position geographically based by or in Wilmington, NC. If you know of any leads pass them along and I will forward to her. Thank you in advance! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Heather.D.Renko <@t> osfhealthcare.org Wed Nov 11 12:25:51 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Nov 11 12:26:05 2009 Subject: [Histonet] re: H. Pylori Message-ID: We are very pleased with the Ventana pre dilute and if we purchased controls, I would recommend Master Scientific as we have always been very pleased with them as a whole. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From LRaff <@t> uropartners.com Wed Nov 11 12:26:33 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Nov 11 12:26:37 2009 Subject: [Histonet] Anyone know any microbiologists? Message-ID: Our histo section is doing well, but if anyone in the Chicago area knows of any microbiology techs looking for part time work, please pass our lab info to them. Thanks, Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 From wlecorch <@t> rwjuhh.edu Wed Nov 11 12:58:29 2009 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Wed Nov 11 12:58:41 2009 Subject: [Histonet] RPMI Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78A75@HAMEXMBA.rwjham.local> For those of you who attended the mini-learn and earn in VA. The RPMI is manufactured by Lonza we purchase from Fisher cat# BW12-167F BioWhittaker RPMI-1640 storage temp is 15 to 30 C / 59 to 86 F From JLINDA <@t> clemson.edu Wed Nov 11 13:29:45 2009 From: JLINDA <@t> clemson.edu (Linda Jenkins) Date: Wed Nov 11 13:29:46 2009 Subject: [Histonet] (no subject) Message-ID: <2E26E8EC61C4D041869EDD2921DD211D95F6DACA14@EXCH07.CAMPUS.CU.CLEMSON.EDU> Dear All, Dr. Karen Burg (JOH editor-in-chief) asked me to forward this reminder to you: The Journal of Histotechnology (JOH) editors, Editorial Board, and guest editor Dr. Richard Cartun are pleased to announce a call for manuscripts for the March 2010 special issue of JOH, with focus on Immunohistochemistry and In Situ Hybridization. Technical notes and methodology-focused contributions are particularly encouraged; clinical, research, and veterinary topics are welcome. Manuscripts must be submitted prior to November 30, 2009 in order to be considered for the March issue. Manuscripts may be sent to Ms. Jane Jacobi, Managing Editor, at joh@nsh.org. Authors wishing pre-submission guidance with the writing process may contact Ms. Gayle Callis, JOH Assistant Editor, at gayle.callis@bresnan.net, for assignment of a writing partner. Information for authors is detailed at www.nsh.org under Journal of Histotechnology. Thanks, Linda Linda Jenkins, HT Clemson University Department of Bioengineering Clemson, SC 29634 Phone: 864-656-5553 Fax: 864-656-4466 email: jlinda@clemson.edu From liz <@t> premierlab.com Wed Nov 11 16:12:13 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Nov 11 16:12:17 2009 Subject: [Histonet] plastic notebook holders for slides Message-ID: Hello All I'm posting this question for a friend of mine. Their pathologist wants to file slides in a notebook and so they need to purchase those plastic slide holders that fit into notebooks, does anyone know who sells those? Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From Andrew.Prior <@t> Smith-Nephew.com Thu Nov 12 04:28:16 2009 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu Nov 12 04:31:06 2009 Subject: [Histonet] Sanderson's RBS Message-ID: <6C18ADDF244BF8439412C063019CFFEC0BBC8107@EHS021.wound.san> I'm looking for a new supplier for Sanderson's Rapid Bone Stain as Surgipath have stopped selling it (at least in Europe). Does anyone know of an alternative supplier or an equivalent stain I could substitute in? [Currently use the RBS on 15?m ground Technovit 7200VLC resin sections, with acid fuchsin counter-stain.] Thanks in advance Andrew Andrew Prior Histologist Smith &Nephew Research Centre Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From ratliffjack <@t> hotmail.com Thu Nov 12 05:52:23 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Nov 12 05:52:20 2009 Subject: [Histonet] Sanderson's RBS In-Reply-To: <6C18ADDF244BF8439412C063019CFFEC0BBC8107@EHS021.wound.san> References: <6C18ADDF244BF8439412C063019CFFEC0BBC8107@EHS021.wound.san> Message-ID: Andrew, I will be updating the Hard Tissue Committee members regarding this very soon but this is very funny that the subject has come up yet again. In fact, I have received a few phone calls over the past couple of weeks on the subject and ironically two yesterday. Addittionally, this was of great concern to me because I too depend upon this product and of concern to several HTC committee members when we met at NSH and discovered Surgipath was no longer going to carry the stain. Nevertheless, I have been told that this stain will now be available via Dorn and Hart Microedge???? Evidently Dorn and Hart is in the process of expanding beyond the services of providing and resharpening knives. In fact, I spoke with the son (Bill Hart) and I was told that Sanderson's Rapid Bone Stain is just the start of many exciting things they will begin to offer "hard tissue" folks in the coming weeks and months. Rest assurred that I will keep committee members informed of the availability of this stain and this new direction of D&H as more information becomes available. Jack Ratliff NSH Hard Tissue Committe Chair On Nov 12, 2009, at 4:28 AM, "Prior, Andrew" wrote: > I'm looking for a new supplier for Sanderson's Rapid Bone Stain as > Surgipath have stopped selling it (at least in Europe). > Does anyone know of an alternative supplier or an equivalent stain I > could substitute in? > [Currently use the RBS on 15?m ground Technovit 7200VLC resin sectio > ns, with acid fuchsin counter-stain.] > > Thanks in advance > Andrew > > Andrew Prior > Histologist > Smith &Nephew Research Centre > Confidentiality. > This electronic transmission is strictly confidential to Smith & > Nephew and intended solely for the addressee. It may contain > information which is covered by legal, professional or other > privilege. If you are not the intended addressee, or someone > authorised by the intended addressee to receive transmissions on > behalf of the addressee, you must not retain, disclose in any form, > copy or take any action in reliance on this transmission. If you > have received this transmission in error, please notify the sender > as soon as possible and destroy this message. > > Smith & Nephew UK > Registered in England and Wales No.4421171 with registered office at > 15 Adam Street, London WC2N 6LA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nancy_schmitt <@t> pa-ucl.com Thu Nov 12 07:41:10 2009 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Nov 12 07:41:17 2009 Subject: [Histonet] CDX2 Message-ID: <737BD0BF52F0744B96B74B61756AC064414603E910@hestia.ad.pa-ucl.com> Good Morning- Need some advice for this stain. We are having trouble with severely weakened stain. We run on the Leica Bond using Epitope Retrieval 2 for 30 minutes. We recently changed from ER2/20 and saw improvement. Ran a trial on stock control blocks (which are kept refrigerated) and on a current case block; current block stained great - stock blocks not so much. We have had success with this in the past, am looking for some guidance. Thanks Nancy Schmitt HT(ASCP), MLT(CSMLS) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext.142 nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From sbreeden <@t> nmda.nmsu.edu Thu Nov 12 08:20:51 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Nov 12 08:20:57 2009 Subject: [Histonet] Akemi - Urgent! Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46C2B@nmdamailsvr.nmda.ad.nmsu.edu> Akemi Allison-Tacha - email me immediately, please. I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From hfedor <@t> jhmi.edu Thu Nov 12 08:29:56 2009 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Nov 12 08:30:01 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46C2B@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46C2B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316B939C455@JHEMTEXVS3.win.ad.jhu.edu> I also receive one of the scam emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please. I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Masterson_John <@t> Allergan.com Thu Nov 12 09:18:42 2009 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Thu Nov 12 09:19:17 2009 Subject: [Histonet] Job opening, Irvine Ca Message-ID: <0C58C4F16F0B67448318A38041CADE4B03DA56B0@IRMAIL133.irvine.allergan.com> We have a job opening here at Allergan Inc. in Irvine CA. You can find the job posting on the Allergan web page or the NSH jobs site. Thanks, John

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation.

From PMonfils <@t> Lifespan.org Thu Nov 12 09:26:48 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Nov 12 09:26:53 2009 Subject: [Histonet] Looking for VEGF and FGF-2 antibodies Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835D04@LSRIEXCH1.lsmaster.lifespan.org> I am having a difficult time finding two antibodies I need. Two companies I ordered them from have discontinued them. VEGF and FGF-2 The tissue is human. Monoclonals preferred but at this point I'll use polyclonals if necessary. Does anyone know a vendor who has them? Thanks. From godsgalnow <@t> aol.com Thu Nov 12 09:30:11 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Nov 12 09:30:39 2009 Subject: [Histonet] Helicobacter pylori IHC stain In-Reply-To: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> Message-ID: <8CC31C4289FF64C-928C-8D3@webmail-m073.sysops.aol.com> We use Biocare's Rabbit Polyclonal (1:150) with the red chromagen....really cleaned up all the background......the docs love it! Roxanne -----Original Message----- From: Taylor, Jean To: 'ihcrg@googlegroups.com' ; 'histonet@lists.utsouthwestern.edu' Sent: Tue, Nov 10, 2009 4:41 pm Subject: [Histonet] Helicobacter pylori IHC stain Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter ylori antibody from, as well as any information including antibody clone, ilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC HC Tech eriter Health Services adison, WI ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Nov 12 10:03:21 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Nov 12 10:03:29 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D316B939C455@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <717402.61836.qm@web113817.mail.gq1.yahoo.com> Hi All, I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; "FISHING FOR MONEY". ?I did in NO WAY send this e-mail! ?Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name. ?THIS IS SCARY! ? I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. ?I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. ?I received a reply that they would check into it. ?This morning I couldn't get into my e-mail account so I had to jump a bunch of hoops and reset my password. ?I hope none of you have this happen to you. The Yahoo Account warning stated: "Yahoo Mail has discovered series of illegal attempts on your?Yahoo Account?from bad?Ip Location?and will shut your account as it has been flagged as a spam account.?Filling Correct Information Carefully and Sending to?Yahoo?Alert Center:" I hope this was legitimate! ?The information they wanted was my user name, password and birthday. Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Helen Fedor wrote: From: Helen Fedor Subject: [Histonet] RE: Akemi - Urgent! To: "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" Date: Thursday, November 12, 2009, 6:29 AM I also receive one of the scam emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please.? I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kblack <@t> digestivehlth.com Thu Nov 12 10:05:31 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Thu Nov 12 10:05:42 2009 Subject: [Histonet] Helicobacter pylori IHC stain References: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> <8CC31C4289FF64C-928C-8D3@webmail-m073.sysops.aol.com> Message-ID: <894999B3232E4D838528FC473ADD1381@digestivehlth.com> We are also trying Biocare's mouse monoclonal with red chromagen. Organisms look like HP on steroids! Beautiful... Konni Black ----- Original Message ----- From: To: ; ; Sent: Thursday, November 12, 2009 7:30 AM Subject: Re: [Histonet] Helicobacter pylori IHC stain > > We use Biocare's Rabbit Polyclonal (1:150) with the red > chromagen....really cleaned up all the background......the docs love it! > > Roxanne > > > -----Original Message----- > From: Taylor, Jean > To: 'ihcrg@googlegroups.com' ; > 'histonet@lists.utsouthwestern.edu' > Sent: Tue, Nov 10, 2009 4:41 pm > Subject: [Histonet] Helicobacter pylori IHC stain > > > > Hi everyone, > Just trying to get an idea of where labs are purchasing their helicobacter > ylori antibody from, as well as any information including antibody clone, > ilution, detection, etc. I currently run the DAKO platform. > Thank you! > Jean Taylor, HT(ASCP)QIHC > HC Tech > eriter Health Services > adison, WI > ______________________________________________ > istonet mailing list > istonet@lists.utsouthwestern.edu > ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Thu Nov 12 10:15:21 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Nov 12 10:15:30 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <717402.61836.qm@web113817.mail.gq1.yahoo.com> References: <717402.61836.qm@web113817.mail.gq1.yahoo.com> Message-ID: <4AFC3499.6000007@umdnj.edu> > I hope this was legitimate! The information they wanted was my user name, password and birthday. yeah, sounds legit to me... lol... Akemi Allison-Tacha wrote: > Hi All, > I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; "FISHING FOR MONEY". I did in NO WAY send this e-mail! Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name. THIS IS SCARY! > I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. I received a reply that they would check into it. This morning I couldn't get into my e-mail account so I had to jump a bunch of hoops and reset my password. I hope none of you have this happen to you. > The Yahoo Account warning stated: "Yahoo Mail has discovered series of illegal attempts on your Yahoo Account from bad Ip Location and will shut your account as it has been flagged as a spam account. Filling Correct Information Carefully and Sending to Yahoo Alert Center:" > I hope this was legitimate! The information they wanted was my user name, password and birthday. > Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL > E-Mail: akemiat3377@yahoo.com > > --- On Thu, 11/12/09, Helen Fedor wrote: > > From: Helen Fedor > Subject: [Histonet] RE: Akemi - Urgent! > To: "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" > Date: Thursday, November 12, 2009, 6:29 AM > > I also receive one of the scam emails. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, November 12, 2009 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Akemi - Urgent! > > Akemi Allison-Tacha - email me immediately, please. I think you are > being scammed and I don't want to email you directly. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From deb.vaneyck <@t> phci.org Thu Nov 12 10:26:15 2009 From: deb.vaneyck <@t> phci.org (Van Eyck, Deb) Date: Thu Nov 12 10:26:46 2009 Subject: [IHCRG] Re: [Histonet] Helicobacter pylori IHC stain In-Reply-To: <8CC31C4289FF64C-928C-8D3@webmail-m073.sysops.aol.com> References: <466B666475DE6547BBB0641E540A4BB504C3F5C58F@EXVS1.meriter.com> <8CC31C4289FF64C-928C-8D3@webmail-m073.sysops.aol.com> Message-ID: <37212590E341574B96E6796D27DF42DAB04C4F@RWDEX3.WHS.phci.org> Both the Dako concentrate at 1: 300 with pH 6 target retrieval and the Cell Marque rtu which is IVD not ASR by the way with ph 6.0 traget retrieval are very clean also. ________________________________ From: godsgalnow@aol.com [mailto:godsgalnow@aol.com] Sent: Thursday, November 12, 2009 9:30 AM To: jtaylor@meriter.com; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] Re: [Histonet] Helicobacter pylori IHC stain We use Biocare's Rabbit Polyclonal (1:150) with the red chromagen....really cleaned up all the background......the docs love it! Roxanne -----Original Message----- From: Taylor, Jean To: 'ihcrg@googlegroups.com' ; 'histonet@lists.utsouthwestern.edu' Sent: Tue, Nov 10, 2009 4:41 pm Subject: [Histonet] Helicobacter pylori IHC stain Hi everyone, Just trying to get an idea of where labs are purchasing their helicobacter pylori antibody from, as well as any information including antibody clone, dilution, detection, etc. I currently run the DAKO platform. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ***Please be advised on November 16th 2009 Prohealth Care will be upgrading to ZixDirect for email encryption. ZixDirect offers an easy to use method for sending and retrieving encrypted email messages. Registration for ZixDirect will be required.*** From leiker <@t> buffalo.edu Thu Nov 12 10:42:27 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Nov 12 10:42:36 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <4AFC3499.6000007@umdnj.edu> References: <717402.61836.qm@web113817.mail.gq1.yahoo.com> <4AFC3499.6000007@umdnj.edu> Message-ID: <4CA58BD57FCE4DECDBFA797D@CDYwxp1931.ad.med.buffalo.edu> Akemi - Thanks for passing this on, even if it is legit. Good to be aware of what's out there. I have heard of email viruses that will send out emails from your account to contacts in your directory, (this would include Histonet...) Regards, Merced --On Thursday, November 12, 2009 11:15 AM -0500 Peter Carroll wrote: >> I hope this was legitimate! The information they wanted was my user >> name, password and birthday. > > yeah, sounds legit to me... lol... > > > > > Akemi Allison-Tacha wrote: >> Hi All, >> I received an e-mail and an early morning call from my friend Helen >> Hedor this morning stating that she received a e-mail scam from me >> stating; "FISHING FOR MONEY". I did in NO WAY send this e-mail! Please >> do not in anyway answer or send money to the bogus e-mail scam which was >> sent in my name. THIS IS SCARY! I don't know what has happened, but I >> came home last night and my yahoo account sent me an e-mail which stated >> that my e-mail has been compromised for scam purposes, and if I didn't >> answer the questions below I would have my account frozen. I >> immediately replied and answered the questions, but then I thought, what >> if this is a scam to extract personal information, so I copied the the >> questionnaire and sent it to the yahoo alert center. I received a reply >> that they would check into it. This morning I couldn't get into my >> e-mail account so I had to jump a bunch of hoops and reset my password. >> I hope none of you have this happen to you. The Yahoo Account warning >> stated: "Yahoo Mail has discovered series of illegal attempts on your >> Yahoo Account from bad Ip Location and will shut your account as it has >> been flagged as a spam account. Filling Correct Information Carefully >> and Sending to Yahoo Alert Center:" I hope this was legitimate! The >> information they wanted was my user name, password and birthday. >> Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL >> E-Mail: akemiat3377@yahoo.com >> >> --- On Thu, 11/12/09, Helen Fedor wrote: >> >> From: Helen Fedor >> Subject: [Histonet] RE: Akemi - Urgent! >> To: "Breeden, Sara" , >> "histonet@lists.utsouthwestern.edu" >> Date: Thursday, November 12, 2009, 6:29 AM >> >> I also receive one of the scam emails. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, >> Sara Sent: Thursday, November 12, 2009 9:21 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Akemi - Urgent! >> >> Akemi Allison-Tacha - email me immediately, please. I think you are >> being scammed and I don't want to email you directly. >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 4700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From rjbuesa <@t> yahoo.com Thu Nov 12 11:04:11 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 12 11:04:15 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <717402.61836.qm@web113817.mail.gq1.yahoo.com> Message-ID: <557533.97007.qm@web65703.mail.ac4.yahoo.com> Akemi: And that was your second mistake. Why the server, in this case Yahoo, would like your password and your birth day when that is not originally needed? I NEVER EVER answer anything even if I am told that my life depends on it. Never answer a request for information of any kind. If it a legitimate request they will call you on the phone. Ren? J. --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: From: Akemi Allison-Tacha Subject: Re: [Histonet] RE: Akemi - Urgent! To: "SaraBreeden" , "histonet@lists.utsouthwestern.edu" , "Helen Fedor" Date: Thursday, November 12, 2009, 11:03 AM Hi All, I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; "FISHING FOR MONEY". ?I did in NO WAY send this e-mail! ?Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name.. ?THIS IS SCARY! ? I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. ?I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. ?I received a reply that they would check into it. ?This morning I couldn't get into my e-mail account so I had to jump a bunch of hoops and reset my password. ?I hope none of you have this happen to you. The Yahoo Account warning stated: "Yahoo Mail has discovered series of illegal attempts on your?Yahoo Account?from bad?Ip Location?and will shut your account as it has been flagged as a spam account.?Filling Correct Information Carefully and Sending to?Yahoo?Alert Center:" I hope this was legitimate! ?The information they wanted was my user name, password and birthday. Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Helen Fedor wrote: From: Helen Fedor Subject: [Histonet] RE: Akemi - Urgent! To: "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" Date: Thursday, November 12, 2009, 6:29 AM I also receive one of the scam emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please.? I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Nov 12 11:05:05 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Nov 12 11:05:10 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <717402.61836.qm@web113817.mail.gq1.yahoo.com> References: <3201CF51728F6048A24FA3AFFFEEF1D316B939C455@JHEMTEXVS3.win.ad.jhu.edu> <717402.61836.qm@web113817.mail.gq1.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8354F@EMAIL.archildrens.org> This happened to one my techs too with his hotmail account. It took him a lot of time to prove to hotmail what had happened and to get his account reopened. He was supposedly in London and needed money. Several of his friends called him and that was how he was alerted to the scam. Be careful. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, November 12, 2009 10:03 AM To: SaraBreeden; histonet@lists.utsouthwestern.edu; Helen Fedor Subject: Re: [Histonet] RE: Akemi - Urgent! Hi All, I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; "FISHING FOR MONEY". ?I did in NO WAY send this e-mail! ?Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name. ?THIS IS SCARY! ? I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. ?I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. ?I received a reply that they would check into it. ?This morning I couldn't get into my e-mail account so I had to jump a bunch of hoops and reset my password. ?I hope none of you have this happen to you. The Yahoo Account warning stated: "Yahoo Mail has discovered series of illegal attempts on your?Yahoo Account?from bad?Ip Location?and will shut your account as it has been flagged as a spam account.?Filling Correct Information Carefully and Sending to?Yahoo?Alert Center:" I hope this was legitimate! ?The information they wanted was my user name, password and birthday. Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Helen Fedor wrote: From: Helen Fedor Subject: [Histonet] RE: Akemi - Urgent! To: "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" Date: Thursday, November 12, 2009, 6:29 AM I also receive one of the scam emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please.? I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From anonwums1 <@t> gmail.com Thu Nov 12 11:13:55 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Nov 12 11:14:03 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <557533.97007.qm@web65703.mail.ac4.yahoo.com> References: <717402.61836.qm@web113817.mail.gq1.yahoo.com> <557533.97007.qm@web65703.mail.ac4.yahoo.com> Message-ID: <858249120911120913m60f3fad2w630faad2c979e0b5@mail.gmail.com> Phishing also happens over the phone as well. It is not unheard of that people get phone calls claiming to be from a bank asking for personal information (why would a bank need your account number... aren't they your bank and don't they already have this information?). It is generally a very bad idea to give any personal information out in any venue other than the physical office of the institution which you do business. If the company suspects that your account has been compromised, they will disable it and then ask you to contact them. If you receive such an e-mail, do not click on any links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa wrote: > Akemi: > And that was your second mistake. Why the server, in this case Yahoo, would > like your password and your birth day when that is not originally needed? > I NEVER EVER answer anything even if I am told that my life depends on it. > Never answer a request for information of any kind. If it a legitimate > request they will call you on the phone. > Ren? J. > > --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: > > > From: Akemi Allison-Tacha > Subject: Re: [Histonet] RE: Akemi - Urgent! > To: "SaraBreeden" , " > histonet@lists.utsouthwestern.edu" , > "Helen Fedor" > Date: Thursday, November 12, 2009, 11:03 AM > > > Hi All, > I received an e-mail and an early morning call from my friend Helen Hedor > this morning stating that she received a e-mail scam from me stating; > "FISHING FOR MONEY". I did in NO WAY send this e-mail! Please do not in > anyway answer or send money to the bogus e-mail scam which was sent in my > name.. THIS IS SCARY! > I don't know what has happened, but I came home last night and my yahoo > account sent me an e-mail which stated that my e-mail has been compromised > for scam purposes, and if I didn't answer the questions below I would have > my account frozen. I immediately replied and answered the questions, but > then I thought, what if this is a scam to extract personal information, so I > copied the the questionnaire and sent it to the yahoo alert center. I > received a reply that they would check into it. This morning I couldn't get > into my e-mail account so I had to jump a bunch of hoops and reset my > password. I hope none of you have this happen to you. > The Yahoo Account warning stated: "Yahoo Mail has discovered series of > illegal attempts on your Yahoo Account from bad Ip Location and will shut > your account as it has been flagged as a spam account. Filling Correct > Information Carefully and Sending to Yahoo Alert Center:" > I hope this was legitimate! The information they wanted was my user name, > password and birthday. > Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL > E-Mail: akemiat3377@yahoo.com > > --- On Thu, 11/12/09, Helen Fedor wrote: > > From: Helen Fedor > Subject: [Histonet] RE: Akemi - Urgent! > To: "Breeden, Sara" , " > histonet@lists.utsouthwestern.edu" > Date: Thursday, November 12, 2009, 6:29 AM > > I also receive one of the scam emails. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, November 12, 2009 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Akemi - Urgent! > > Akemi Allison-Tacha - email me immediately, please. I think you are > being scammed and I don't want to email you directly. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Nov 12 11:26:02 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Nov 12 11:26:07 2009 Subject: [Histonet] RE: This is what was sent Akemi - Urgent! In-Reply-To: <858249120911120913m60f3fad2w630faad2c979e0b5@mail.gmail.com> Message-ID: <179188.20216.qm@web113810.mail.gq1.yahoo.com> This is the Yahoo ?ALERT that was sent it looks legitimate;From: ? basantakd@yahoo.com Subject: Dear Valued Member, Date: November 11, 2009 10:49:15 AM PST To: ? undisclosed recipients: ; Reply-To:?messagecenter2220@live.comAccount Alert Dear Valued Member, Yahoo Mail has discovered series of illegal attempts on your?Yahoo Account?from bad?Ip Location?and will shut your account as it has been flagged as a spam account. You are the thus immediately required to Secure Your Online Access by Manually Filling The Form Below by clicking on the reply-to button on your page, Filling Correct Information Carefully and Sending to?Yahoo?Alert Center:?UserName:?...................Password: .......................Date of Birth: ......................Country Or Territory : ................... ?After following the instructions in the sheet, your account will not be interrupted and will continue as normal. Thanks for your attention to this request. We apologize for any inconvenience. ?Warning!!! ?Account owner that refuses to update his or her account before two weeks of receiving this warning will lose his or her account permanently. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingE-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Adam . wrote: From: Adam . Subject: Re: [Histonet] RE: Akemi - Urgent! To: histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 9:13 AM Phishing also happens over the phone as well. It is not unheard of that people get phone calls claiming to be from a bank asking for personal information (why would a bank need your account number... aren't they your bank and don't they already have this information?). It is generally a very bad idea to give any personal information out in any venue other than the physical office of the institution which you do business. If the company suspects that your account has been compromised, they will disable it and then ask you to contact them. If you receive such an e-mail, do not click on any links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa wrote: > Akemi: > And that was your second mistake. Why the server, in this case Yahoo, would > like your password and your birth day when that is not originally needed? > I NEVER EVER answer anything even if I am told that my life depends on it. > Never answer a request for information of any kind. If it a legitimate > request they will call you on the phone. > Ren? J. > > --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: > > > From: Akemi Allison-Tacha > Subject: Re: [Histonet] RE: Akemi - Urgent! > To: "SaraBreeden" , " > histonet@lists.utsouthwestern.edu" , > "Helen Fedor" > Date: Thursday, November 12, 2009, 11:03 AM > > > Hi All, > I received an e-mail and an early morning call from my friend Helen Hedor > this morning stating that she received a e-mail scam from me stating; > "FISHING FOR MONEY".? I did in NO WAY send this e-mail!? Please do not in > anyway answer or send money to the bogus e-mail scam which was sent in my > name..? THIS IS SCARY! > I don't know what has happened, but I came home last night and my yahoo > account sent me an e-mail which stated that my e-mail has been compromised > for scam purposes, and if I didn't answer the questions below I would have > my account frozen.? I immediately replied and answered the questions, but > then I thought, what if this is a scam to extract personal information, so I > copied the the questionnaire and sent it to the yahoo alert center.? I > received a reply that they would check into it.? This morning I couldn't get > into my e-mail account so I had to jump a bunch of hoops and reset my > password.? I hope none of you have this happen to you. > The Yahoo Account warning stated: "Yahoo Mail has discovered series of > illegal attempts on your Yahoo Account from bad Ip Location and will shut > your account as it has been flagged as a spam account. Filling Correct > Information Carefully and Sending to Yahoo Alert Center:" > I hope this was legitimate!? The information they wanted was my user name, > password and birthday. > Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL > E-Mail: akemiat3377@yahoo.com > > --- On Thu, 11/12/09, Helen Fedor wrote: > > From: Helen Fedor > Subject: [Histonet] RE: Akemi - Urgent! > To: "Breeden, Sara" , " > histonet@lists.utsouthwestern.edu" > Date: Thursday, November 12, 2009, 6:29 AM > > I also receive one of the scam emails. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, November 12, 2009 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Akemi - Urgent! > > Akemi Allison-Tacha - email me immediately, please.? I think you are > being scammed and I don't want to email you directly. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM? 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Nov 12 10:36:38 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Nov 12 11:28:04 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <717402.61836.qm@web113817.mail.gq1.yahoo.com> References: <717402.61836.qm@web113817.mail.gq1.yahoo.com> Message-ID: <4A954624-0D34-4BE2-9B3C-584260FC7F04@email.arizona.edu> The information they wanted was my user name, password and birthday You should NEVER give out this info - if it is legit Yahoo would have what they need to investigate. I think you are being scammed. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Nov 12, 2009, at 9:03 AM, Akemi Allison-Tacha wrote: > The information they wanted was my user name, password and birthday From WBENTON <@t> umm.edu Thu Nov 12 11:29:13 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Thu Nov 12 11:29:34 2009 Subject: [Histonet] Pastic notebook holders for slides In-Reply-To: <67DFBFA4.396@GWIA1.umm.edu> References: <67DFBFA4.396@GWIA1.umm.edu> Message-ID: <4AFBFF96.D886.00F4.3@umm.edu> Liz, Fisher Scientific carries this product 22088724 Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From victor <@t> pathology.washington.edu Thu Nov 12 11:52:01 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Nov 12 11:52:06 2009 Subject: [Histonet] RE: This is what was sent Akemi - Urgent! In-Reply-To: <179188.20216.qm@web113810.mail.gq1.yahoo.com> References: <179188.20216.qm@web113810.mail.gq1.yahoo.com> Message-ID: <4AFC4B41.5040106@pathology.washington.edu> Here is helpful information from Yahoo. Akemi you will want to contact Yahoo ASAP. http://safely.yahoo.com/faq Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Akemi Allison-Tacha wrote: > This is the Yahoo ALERT that was sent it looks legitimate;From: basantakd@yahoo.com > Subject: Dear Valued Member, > Date: November 11, 2009 10:49:15 AM PST > To: undisclosed recipients: ; > Reply-To: messagecenter2220@live.comAccount Alert > > Dear Valued Member, > Yahoo Mail has discovered series of illegal attempts on your Yahoo Account from bad Ip Location and will shut your account as it has been flagged as a spam account. You are the thus immediately required to Secure Your Online Access by Manually Filling The Form Below by clicking on the reply-to button on your page, Filling Correct Information Carefully and Sending to Yahoo Alert Center: UserName: ...................Password: .......................Date of Birth: ......................Country Or Territory : ................... > > After following the instructions in the sheet, your account will not be interrupted and will continue as normal. Thanks for your attention to this request. We apologize for any inconvenience. > Warning!!! Account owner that refuses to update his or her account before two weeks of receiving this warning will lose his or her account permanently. > Akemi Allison-Tacha BS, HT(ASCP)HTL > PresidentPhoenix Lab ConsultingE-Mail: akemiat3377@yahoo.com > > > > --- On Thu, 11/12/09, Adam . wrote: > > From: Adam . > Subject: Re: [Histonet] RE: Akemi - Urgent! > To: histonet@lists.utsouthwestern.edu > Date: Thursday, November 12, 2009, 9:13 AM > > Phishing also happens over the phone as well. It is not unheard of that > people get phone calls claiming to be from a bank asking for personal > information (why would a bank need your account number... aren't they your > bank and don't they already have this information?). It is generally a very > bad idea to give any personal information out in any venue other than the > physical office of the institution which you do business. If the company > suspects that your account has been compromised, they will disable it and > then ask you to contact them. If you receive such an e-mail, do not click on > any links in it or dial any phone number contained within. Instead, look up > the company's contact information (in a phone book or via a search engine) > and use the publicly available contact information. > > Adam > > On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa wrote: > > >> Akemi: >> And that was your second mistake. Why the server, in this case Yahoo, would >> like your password and your birth day when that is not originally needed? >> I NEVER EVER answer anything even if I am told that my life depends on it. >> Never answer a request for information of any kind. If it a legitimate >> request they will call you on the phone. >> Ren? J. >> >> --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: >> >> >> From: Akemi Allison-Tacha >> Subject: Re: [Histonet] RE: Akemi - Urgent! >> To: "SaraBreeden" , " >> histonet@lists.utsouthwestern.edu" , >> "Helen Fedor" >> Date: Thursday, November 12, 2009, 11:03 AM >> >> >> Hi All, >> I received an e-mail and an early morning call from my friend Helen Hedor >> this morning stating that she received a e-mail scam from me stating; >> "FISHING FOR MONEY". I did in NO WAY send this e-mail! Please do not in >> anyway answer or send money to the bogus e-mail scam which was sent in my >> name.. THIS IS SCARY! >> I don't know what has happened, but I came home last night and my yahoo >> account sent me an e-mail which stated that my e-mail has been compromised >> for scam purposes, and if I didn't answer the questions below I would have >> my account frozen. I immediately replied and answered the questions, but >> then I thought, what if this is a scam to extract personal information, so I >> copied the the questionnaire and sent it to the yahoo alert center. I >> received a reply that they would check into it. This morning I couldn't get >> into my e-mail account so I had to jump a bunch of hoops and reset my >> password. I hope none of you have this happen to you. >> The Yahoo Account warning stated: "Yahoo Mail has discovered series of >> illegal attempts on your Yahoo Account from bad Ip Location and will shut >> your account as it has been flagged as a spam account. Filling Correct >> Information Carefully and Sending to Yahoo Alert Center:" >> I hope this was legitimate! The information they wanted was my user name, >> password and birthday. >> Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL >> E-Mail: akemiat3377@yahoo.com >> >> --- On Thu, 11/12/09, Helen Fedor wrote: >> >> From: Helen Fedor >> Subject: [Histonet] RE: Akemi - Urgent! >> To: "Breeden, Sara" , " >> histonet@lists.utsouthwestern.edu" >> Date: Thursday, November 12, 2009, 6:29 AM >> >> I also receive one of the scam emails. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara >> Sent: Thursday, November 12, 2009 9:21 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Akemi - Urgent! >> >> Akemi Allison-Tacha - email me immediately, please. I think you are >> being scammed and I don't want to email you directly. >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 4700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JPeters <@t> bostwicklaboratories.com Thu Nov 12 11:52:22 2009 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Thu Nov 12 11:52:27 2009 Subject: [Histonet] RE: plastic notebook holders for slides (Liz Chlipala) In-Reply-To: References: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F076D5906@mail1.BOSTWICK.COM> You can get them from VWR (item #: 70690-024). 16 slides per page Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com From akemiat3377 <@t> yahoo.com Thu Nov 12 11:56:51 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Nov 12 11:56:56 2009 Subject: [Histonet] RE:Akemi - Urgent! Scammed! In-Reply-To: <1634945237.7552071258047350885.JavaMail.root@md05.embarq.synacor.com> Message-ID: <570575.31317.qm@web113812.mail.gq1.yahoo.com> Well Guys, looks like I have definitely been Scammed and Spammed! ?After I thought about the possible rouse, I did however copy the original Alert that was sent and sent it to the Yahoo Alert Center. ?The REAL Yahoo Alert Center has the CRIMINAL'S e-mail addresses to do their investigation. Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Shawn Leslie wrote: From: Shawn Leslie Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! To: "Akemi Allison-Tacha" Date: Thursday, November 12, 2009, 9:35 AM For one...The return address is a Live address...That's MSN not Yahoo....You are definitely being SCAMMED... ----- Original Message ----- From: "Akemi Allison-Tacha" To: histonet@lists.utsouthwestern.edu, "Adam ." Sent: Thursday, November 12, 2009 12:26:02 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! This is the Yahoo ?ALERT that was sent it looks legitimate;From: ??? ? basantakd@yahoo.com Subject: ??? Dear Valued Member, Date: ??? November 11, 2009 10:49:15 AM PST To: ??? ? undisclosed recipients: ; Reply-To:?messagecenter2220@live.comAccount Alert Dear Valued Member, Yahoo Mail has discovered series of illegal attempts on your?Yahoo Account?from bad?Ip Location?and will shut your account as it has been flagged as a spam account. You are the thus immediately required to Secure Your Online Access by Manually Filling The Form Below by clicking on the reply-to button on your page, Filling Correct Information Carefully and Sending to?Yahoo?Alert Center:?UserName:?...................Password: .......................Date of Birth: ......................Country Or Territory : ................... ?After following the instructions in the sheet, your account will not be interrupted and will continue as normal. Thanks for your attention to this request. We apologize for any inconvenience. ?Warning!!! ?Account owner that refuses to update his or her account before two weeks of receiving this warning will lose his or her account permanently. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingE-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Adam . wrote: From: Adam . Subject: Re: [Histonet] RE: Akemi - Urgent! To: histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 9:13 AM Phishing also happens over the phone as well. It is not unheard of that people get phone calls claiming to be from a bank asking for personal information (why would a bank need your account number... aren't they your bank and don't they already have this information?). It is generally a very bad idea to give any personal information out in any venue other than the physical office of the institution which you do business. If the company suspects that your account has been compromised, they will disable it and then ask you to contact them. If you receive such an e-mail, do not click on any links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa wrote: > Akemi: > And that was your second mistake. Why the server, in this case Yahoo, would > like your password and your birth day when that is not originally needed? > I NEVER EVER answer anything even if I am told that my life depends on it. > Never answer a request for information of any kind. If it a legitimate > request they will call you on the phone. > Ren? J. > > --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: > > > From: Akemi Allison-Tacha > Subject: Re: [Histonet] RE: Akemi - Urgent! > To: "SaraBreeden" , " > histonet@lists.utsouthwestern.edu" , > "Helen Fedor" > Date: Thursday, November 12, 2009, 11:03 AM > > > Hi All, > I received an e-mail and an early morning call from my friend Helen Hedor > this morning stating that she received a e-mail scam from me stating; > "FISHING FOR MONEY".? I did in NO WAY send this e-mail!? Please do not in > anyway answer or send money to the bogus e-mail scam which was sent in my > name..? THIS IS SCARY! > I don't know what has happened, but I came home last night and my yahoo > account sent me an e-mail which stated that my e-mail has been compromised > for scam purposes, and if I didn't answer the questions below I would have > my account frozen.? I immediately replied and answered the questions, but > then I thought, what if this is a scam to extract personal information, so I > copied the the questionnaire and sent it to the yahoo alert center.? I > received a reply that they would check into it.? This morning I couldn't get > into my e-mail account so I had to jump a bunch of hoops and reset my > password.? I hope none of you have this happen to you. > The Yahoo Account warning stated: "Yahoo Mail has discovered series of > illegal attempts on your Yahoo Account from bad Ip Location and will shut > your account as it has been flagged as a spam account. Filling Correct > Information Carefully and Sending to Yahoo Alert Center:" > I hope this was legitimate!? The information they wanted was my user name, > password and birthday. > Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL > E-Mail: akemiat3377@yahoo.com > > --- On Thu, 11/12/09, Helen Fedor wrote: > > From: Helen Fedor > Subject: [Histonet] RE: Akemi - Urgent! > To: "Breeden, Sara" , " > histonet@lists.utsouthwestern.edu" > Date: Thursday, November 12, 2009, 6:29 AM > > I also receive one of the scam emails. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, November 12, 2009 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Akemi - Urgent! > > Akemi Allison-Tacha - email me immediately, please.? I think you are > being scammed and I don't want to email you directly. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM? 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Nov 12 12:08:56 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 12 12:09:01 2009 Subject: [Histonet] RE: This is what was sent Akemi - Urgent! In-Reply-To: <179188.20216.qm@web113810.mail.gq1.yahoo.com> Message-ID: <822428.49027.qm@web65709.mail.ac4.yahoo.com> Please Akemi, give me a break! "Dear Valued Member"? Don't they even know WHO you are? It is the same as receiving a junk mail addressed to you or to the?"Current Resident", they don't know. You should not have answered that e-mail. Flag it spam and?move on with your e-mail! Ren? J. --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: From: Akemi Allison-Tacha Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! To: histonet@lists.utsouthwestern.edu, "Adam ." Date: Thursday, November 12, 2009, 12:26 PM This is the Yahoo ?ALERT that was sent it looks legitimate;From: ??? ? basantakd@yahoo.com Subject: ??? Dear Valued Member, Date: ??? November 11, 2009 10:49:15 AM PST To: ??? ? undisclosed recipients: ; Reply-To:?messagecenter2220@live.comAccount Alert Dear Valued Member, Yahoo Mail has discovered series of illegal attempts on your?Yahoo Account?from bad?Ip Location?and will shut your account as it has been flagged as a spam account. You are the thus immediately required to Secure Your Online Access by Manually Filling The Form Below by clicking on the reply-to button on your page, Filling Correct Information Carefully and Sending to?Yahoo?Alert Center:?UserName:?...................Password: ........................Date of Birth: ......................Country Or Territory : ................... ?After following the instructions in the sheet, your account will not be interrupted and will continue as normal. Thanks for your attention to this request. We apologize for any inconvenience. ?Warning!!! ?Account owner that refuses to update his or her account before two weeks of receiving this warning will lose his or her account permanently. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingE-Mail: akemiat3377@yahoo.com --- On Thu, 11/12/09, Adam . wrote: From: Adam . Subject: Re: [Histonet] RE: Akemi - Urgent! To: histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 9:13 AM Phishing also happens over the phone as well. It is not unheard of that people get phone calls claiming to be from a bank asking for personal information (why would a bank need your account number... aren't they your bank and don't they already have this information?). It is generally a very bad idea to give any personal information out in any venue other than the physical office of the institution which you do business. If the company suspects that your account has been compromised, they will disable it and then ask you to contact them. If you receive such an e-mail, do not click on any links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa wrote: > Akemi: > And that was your second mistake. Why the server, in this case Yahoo, would > like your password and your birth day when that is not originally needed? > I NEVER EVER answer anything even if I am told that my life depends on it.. > Never answer a request for information of any kind. If it a legitimate > request they will call you on the phone. > Ren? J. > > --- On Thu, 11/12/09, Akemi Allison-Tacha wrote: > > > From: Akemi Allison-Tacha > Subject: Re: [Histonet] RE: Akemi - Urgent! > To: "SaraBreeden" , " > histonet@lists.utsouthwestern.edu" , > "Helen Fedor" > Date: Thursday, November 12, 2009, 11:03 AM > > > Hi All, > I received an e-mail and an early morning call from my friend Helen Hedor > this morning stating that she received a e-mail scam from me stating; > "FISHING FOR MONEY".? I did in NO WAY send this e-mail!? Please do not in > anyway answer or send money to the bogus e-mail scam which was sent in my > name..? THIS IS SCARY! > I don't know what has happened, but I came home last night and my yahoo > account sent me an e-mail which stated that my e-mail has been compromised > for scam purposes, and if I didn't answer the questions below I would have > my account frozen.? I immediately replied and answered the questions, but > then I thought, what if this is a scam to extract personal information, so I > copied the the questionnaire and sent it to the yahoo alert center.? I > received a reply that they would check into it.? This morning I couldn't get > into my e-mail account so I had to jump a bunch of hoops and reset my > password.? I hope none of you have this happen to you. > The Yahoo Account warning stated: "Yahoo Mail has discovered series of > illegal attempts on your Yahoo Account from bad Ip Location and will shut > your account as it has been flagged as a spam account. Filling Correct > Information Carefully and Sending to Yahoo Alert Center:" > I hope this was legitimate!? The information they wanted was my user name, > password and birthday. > Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL > E-Mail: akemiat3377@yahoo.com > > --- On Thu, 11/12/09, Helen Fedor wrote: > > From: Helen Fedor > Subject: [Histonet] RE: Akemi - Urgent! > To: "Breeden, Sara" , " > histonet@lists.utsouthwestern.edu" > Date: Thursday, November 12, 2009, 6:29 AM > > I also receive one of the scam emails. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Thursday, November 12, 2009 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Akemi - Urgent! > > Akemi Allison-Tacha - email me immediately, please.? I think you are > being scammed and I don't want to email you directly. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM? 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Thu Nov 12 12:47:02 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Thu Nov 12 12:47:49 2009 Subject: [Histonet] Kappa, Lambda Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B68BC@exchangent> Fellow Histonetters, Is there any one using the Leica BOND IHC instrument who is having trouble making their Kappa and Lambda work well on their bone marrow specimens? We are presently using B5 on our bone marrows. Does anybody who uses B5 have this problem? Thanks for your help. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From JWeems <@t> sjha.org Thu Nov 12 12:58:32 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 12 12:58:33 2009 Subject: [Histonet] Kappa, Lambda In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B68BC@exchangent> References: <4F0B7161A6CD524FAD8017D52E1553400D2B68BC@exchangent> Message-ID: What kind of decal are you using? If it is HCL based, it may be too harsh. I wouldn't broadcast the B5 fact to the world!! :>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Thursday, November 12, 2009 13:47 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Kappa, Lambda Fellow Histonetters, Is there any one using the Leica BOND IHC instrument who is having trouble making their Kappa and Lambda work well on their bone marrow specimens? We are presently using B5 on our bone marrows. Does anybody who uses B5 have this problem? Thanks for your help. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From kblack <@t> digestivehlth.com Thu Nov 12 13:19:19 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Thu Nov 12 13:19:29 2009 Subject: [Histonet] Position in Olympia, WA Message-ID: <47F281C2EF214B2F8C93F8FE5755D61C@digestivehlth.com> New lab opening in Olympia, Washington needs a fulltime HT or HTL. GI practice offers benefits, very flexible hours, and ideal working conditions. Must have 3 to 5 years experience. Please contact me if you are interested. Konni Black Pathology/ Lab Development, LLC 253-503-2560 office 253-312-9964 cell 253-682-2433 fx From kblack <@t> digestivehlth.com Thu Nov 12 13:19:24 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Thu Nov 12 13:19:31 2009 Subject: [Histonet] histotech opening St. Augustine, FL Message-ID: <85D66A58FFD74AC29DB6201AE71CAE92@digestivehlth.com> New St. Augustine lab opening in January needs a fulltime HT or HTL with Florida license. GI practice offers benefits, very flexible hours, and ideal working conditions. Must have 3 to 5 years experience. Please contact me if you are interested. Konni Black Pathology/ Lab Development, LLC 253-503-2560 office 253-312-9964 cell 253-682-2433 fx From deliadfam <@t> yahoo.com Thu Nov 12 13:21:24 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Thu Nov 12 13:21:27 2009 Subject: [Histonet] Thanks!!! Re: hematoxylin Message-ID: <89106.38899.qm@web63104.mail.re1.yahoo.com> Thank you all for your responses. I have tried a few things. But still going at it. :-) Have a great day! From carrolpb <@t> umdnj.edu Thu Nov 12 13:35:27 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Nov 12 13:35:40 2009 Subject: [Histonet] RE:Akemi - Urgent! Scammed! In-Reply-To: <570575.31317.qm@web113812.mail.gq1.yahoo.com> References: <1634945237.7552071258047350885.JavaMail.root@md05.embarq.synacor.com> <570575.31317.qm@web113812.mail.gq1.yahoo.com> Message-ID: Aren't you the one who also sent the whole of Histonet an untrue urban-legend email forward a while back? You might want to consider being a tad more careful with your online doings. Especially anything that could tie up an entire mailing list with your indiscretions... ----- Original Message ----- From: Akemi Allison-Tacha Date: Thursday, November 12, 2009 12:57 pm Subject: Re: [Histonet] RE:Akemi - Urgent! Scammed! To: Shawn Leslie , Histonet > Well Guys, looks like I have definitely been Scammed and > Spammed! ?After I thought about the possible rouse, I did > however copy the original Alert that was sent and sent it to the > Yahoo Alert Center. ?The REAL Yahoo Alert Center has the > CRIMINAL'S e-mail addresses to do their investigation. > > Akemi Allison-Tacha BS, HT(ASCP)HTL > E-Mail: akemiat3377@yahoo.com > --- On Thu, 11/12/09, Shawn Leslie > wrote: > > From: Shawn Leslie > Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! > To: "Akemi Allison-Tacha" > Date: Thursday, November 12, 2009, 9:35 AM > > For one...The return address is a Live address...That's MSN not > Yahoo....You are definitely being SCAMMED... > > > ----- Original Message ----- > From: "Akemi Allison-Tacha" > To: histonet@lists.utsouthwestern.edu, "Adam ." > Sent: Thursday, November 12, 2009 > 12:26:02 PM GMT -05:00 US/Canada Eastern > Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! > > This is the Yahoo ?ALERT that was sent it looks legitimate;From: > ??? ? basantakd@yahoo.com > Subject: ??? Dear Valued Member, > Date: ??? November 11, 2009 10:49:15 AM PST > To: ??? ? undisclosed recipients: ; > Reply-To:?messagecenter2220@live.comAccount Alert > > Dear Valued Member, > Yahoo Mail has discovered series of illegal attempts on > your?Yahoo Account?from bad?Ip Location?and will shut your > account as it has been flagged as a spam account. You are the > thus immediately required to Secure Your Online Access by > Manually Filling The Form Below by clicking on the reply-to > button on your page, Filling Correct Information Carefully and > Sending to?Yahoo?Alert > Center:?UserName:?...................Password: .......................Date of Birth: ......................Country Or Territory : ................... > > ?After following the instructions in the sheet, your account > will not be interrupted and will continue as normal. Thanks for > your attention to this request. We apologize for any inconvenience. > ?Warning!!! ?Account owner that refuses to update his or her > account before two weeks of receiving this warning will lose his > or her account permanently. > Akemi Allison-Tacha BS, HT(ASCP)HTL > PresidentPhoenix Lab ConsultingE-Mail: akemiat3377@yahoo.com > > > > --- On Thu, 11/12/09, Adam . wrote: > > From: Adam . > Subject: Re: [Histonet] RE: Akemi - Urgent! > To: histonet@lists.utsouthwestern.edu > Date: Thursday, November 12, 2009, 9:13 AM > > Phishing also happens over the phone as well. It is not unheard > of that > people get phone calls claiming to be from a bank asking for personal > information (why would a bank need your account number... aren't > they your > bank and don't they already have this information?). It is > generally a very > bad idea to give any personal information out in any venue other > than the > physical office of the institution which you do business. If the > companysuspects that your account has been compromised, they > will disable it and > then ask you to contact them. If you receive such an e-mail, do > not click on > any links in it or dial any phone number contained within. > Instead, look up > the company's contact information (in a phone book or via a > search engine) > and use the publicly available contact information. > > Adam > > On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa > wrote: > > > Akemi: > > And that was your second mistake. Why the server, in this case > Yahoo, would > > like your password and your birth day when that is not > originally needed? > > I NEVER EVER answer anything even if I am told that my life > depends on it. > > Never answer a request for information of any kind. If it a > legitimate> request they will call you on the phone. > > Ren? J. > > > > --- On Thu, 11/12/09, Akemi Allison-Tacha > wrote: > > > > > > From: Akemi Allison-Tacha > > Subject: Re: [Histonet] RE: Akemi - Urgent! > > To: "SaraBreeden" , " > > histonet@lists.utsouthwestern.edu" > ,> "Helen Fedor" > > Date: Thursday, November 12, 2009, 11:03 AM > > > > > > Hi All, > > I received an e-mail and an early morning call from my friend > Helen Hedor > > this morning stating that she received a e-mail scam from me > stating;> "FISHING FOR MONEY".? I did in NO WAY send this e- > mail!? Please do not in > > anyway answer or send money to the bogus e-mail scam which was > sent in my > > name..? THIS IS SCARY! > > I don't know what has happened, but I came home last night and > my yahoo > > account sent me an e-mail which stated that my e-mail has been > compromised> for scam purposes, and if I didn't answer the > questions below I would have > > my account frozen.? I immediately replied and answered the > questions, but > > then I thought, what if this is a scam to extract personal > information, so I > > copied the the questionnaire and sent it to the yahoo alert > center.? I > > received a reply that they would check into it.? This morning > I couldn't get > > into my e-mail account so I had to jump a bunch of hoops and > reset my > > password.? I hope none of you have this happen to you. > > The Yahoo Account warning stated: "Yahoo Mail has discovered > series of > > illegal attempts on your Yahoo Account from bad Ip Location > and will shut > > your account as it has been flagged as a spam account. Filling > Correct> Information Carefully and Sending to Yahoo Alert Center:" > > I hope this was legitimate!? The information they wanted was > my user name, > > password and birthday. > > Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL > > E-Mail: akemiat3377@yahoo.com > > > > --- On Thu, 11/12/09, Helen Fedor wrote: > > > > From: Helen Fedor > > Subject: [Histonet] RE: Akemi - Urgent! > > To: "Breeden, Sara" , " > > histonet@lists.utsouthwestern.edu" > > Date: Thursday, November > 12, 2009, 6:29 AM > > > > I also receive one of the scam emails. > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > > Sent: Thursday, November 12, 2009 9:21 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Akemi - Urgent! > > > > Akemi Allison-Tacha - email me immediately, please.? I think > you are > > being scammed and I don't want to email you directly. > > > > > > > > Sally Breeden, HT(ASCP) > > > > NM Dept. of Agriculture > > > > Veterinary Diagnostic Services > > > > PO Box 4700 > > > > Albuquerque, NM? 87106 > > > > 505-841-2576 > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> burnham.org Thu Nov 12 14:49:53 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Thu Nov 12 14:49:58 2009 Subject: [Histonet] Tissue falling off slide Message-ID: Hi Histonetters, This is a second request for help. I know that this is a pretty rudimentary question but I need help. I have is group that has given me 175 mouse heart specimens for processing and embedding and returned the specimens for them to start microtoming them and the tissue is falling off the slide. They brought some blocks back for me to cut and I placed the sections on three types of slides Superfrost Plus, Colorfrost Plus which are basically the same and Polysine slide and still had falling off but not at the degree of how their sections fell off. I was thinking that it could be a water issue in the water bath or a tissue processing problem that may be causing it. I process with isopropanol- 6 stations( 30 minutes each) all 100%-and I placed in xylene- 1 station 40 minutes and three paraffins 45 minutes each @60 degrees. The tissue looked perfect when they came out of the processor and I embedded them. Any help and advice would be appreciated. Thanks!!! John From rjbuesa <@t> yahoo.com Thu Nov 12 15:03:19 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 12 15:03:24 2009 Subject: [Histonet] Tissue falling off slide In-Reply-To: Message-ID: <991541.49480.qm@web65709.mail.ac4.yahoo.com> I think that the tissues have been too long in the alcohol. I would suggest hydrate the tissues and process them again with less time in the? alcohols. Ren? J. --- On Thu, 11/12/09, John Shelley wrote: From: John Shelley Subject: [Histonet] Tissue falling off slide To: "histonet@lists.utsouthwestern.edu" Date: Thursday, November 12, 2009, 3:49 PM Hi Histonetters, This is a second request for help. I know that this is a pretty rudimentary question but I need help. I have is group that has given me 175 mouse heart specimens for processing and embedding and returned the specimens for them to? start microtoming them and the tissue is falling off the slide. They brought some blocks back for me to cut and I placed the sections on three types of slides Superfrost Plus, Colorfrost Plus which are basically the same and Polysine slide and still had falling off but not at the degree of how their sections fell off. I was thinking that it could be a water issue in the water bath or a tissue processing problem that may be causing it. I process with isopropanol- 6 stations( 30 minutes each) all 100%-and I placed in xylene- 1 station 40 minutes and three paraffins 45 minutes each @60 degrees. The tissue looked perfect when they came out of the processor and I embedded them. Any help and advice would be appreciated.? Thanks!!! John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Thu Nov 12 15:04:16 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Nov 12 15:04:21 2009 Subject: [Histonet] RE: Tissue falling off slide In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE222A558ED23@MERCERMAIL.MercerU.local> Hi John, In the past for IHC I used Surgipath StaOn in the waterbath for tissues that I thought may wash, usually the bloody ones. I do not do IHC anymore and found that the positive charged slides I had left over did not work on the autopsy blocks I now cut for the ME. Sta-On works better than any other product I have tried. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Thursday, November 12, 2009 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off slide Hi Histonetters, This is a second request for help. I know that this is a pretty rudimentary question but I need help. I have is group that has given me 175 mouse heart specimens for processing and embedding and returned the specimens for them to start microtoming them and the tissue is falling off the slide. They brought some blocks back for me to cut and I placed the sections on three types of slides Superfrost Plus, Colorfrost Plus which are basically the same and Polysine slide and still had falling off but not at the degree of how their sections fell off. I was thinking that it could be a water issue in the water bath or a tissue processing problem that may be causing it. I process with isopropanol- 6 stations( 30 minutes each) all 100%-and I placed in xylene- 1 station 40 minutes and three paraffins 45 minutes each @60 degrees. The tissue looked perfect when they came out of the processor and I embedded them. Any help and advice would be appreciated. Thanks!!! John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histion <@t> hotmail.com Thu Nov 12 16:39:18 2009 From: histion <@t> hotmail.com (Hotmail) Date: Thu Nov 12 16:39:24 2009 Subject: [Histonet] FW: RIP Russ Allison Message-ID: Vintage histonetters will remember the valuable contributions to our body of knowledge made by Russ Allison. I was saddened to hear of his recent passing after a seven year battle with Alzheimers. I'll not presume to eulogize him, there are many who knew him better and longer, suffice it to say you would have to go a long way to find a kinder, more helpful man with as great a passion for laboratory science, scientific education and Welsh rugby. On his annual trips to the US to present workshops at NSH meetings he would always find time for a pint or two and spirited discussion about the progress of the profession on both sides of the pond. The following is the URL of an obituary of Russ by the current chief executive if the Institute of Biomedical Scientists. http://www.ibms.org/go/media-centre:monthly-comment I'm off to the beer aisle so I can lift a Brains in his honour tonight. Cheers, Russ and farewell. Simon Smith BSc MIBMS Chief Technical Officer Histion LLC 2615 W Casino Rd Unit 6G Everett, WA 98204 smiths@Histion.com T: (206) 953 1386 F: (425) 353-3604 LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorised. If you are not an adressee, any disclosure or copying of the contents of this e-mail or any action taken (or not taken) in reliance on it is unauthorised and may be unlawful. If you are not an addressee, please inform the sender immediately. From dellav <@t> musc.edu Thu Nov 12 17:30:32 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Nov 12 17:30:36 2009 Subject: [Histonet] RE: Akemi - Urgent! In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D316B939C455@JHEMTEXVS3.win.ad.jhu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46C2B@nmdamailsvr.nmda.ad.nmsu.edu> <3201CF51728F6048A24FA3AFFFEEF1D316B939C455@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: I also received the scam message purportedly from Akemi so something is amiss. I can only assume Akemi that your address book/contact list has been acquired by whoever is behind this. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 12, 2009 9:30 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Akemi - Urgent! I also receive one of the scam emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please. I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 12 17:36:06 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Nov 12 17:36:21 2009 Subject: [Histonet] FW: RIP Russ Allison In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4CF76A2DB8@EXMBMCB15.ucsfmedicalcenter.org> I'm sorry to hear that. I met Russ several times at NSH over the years and had several email correspondences with him. He was always helpful and a great guy. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hotmail Sent: Thursday, November 12, 2009 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: RIP Russ Allison Vintage histonetters will remember the valuable contributions to our body of knowledge made by Russ Allison. I was saddened to hear of his recent passing after a seven year battle with Alzheimers. I'll not presume to eulogize him, there are many who knew him better and longer, suffice it to say you would have to go a long way to find a kinder, more helpful man with as great a passion for laboratory science, scientific education and Welsh rugby. On his annual trips to the US to present workshops at NSH meetings he would always find time for a pint or two and spirited discussion about the progress of the profession on both sides of the pond. The following is the URL of an obituary of Russ by the current chief executive if the Institute of Biomedical Scientists. http://www.ibms.org/go/media-centre:monthly-comment I'm off to the beer aisle so I can lift a Brains in his honour tonight. Cheers, Russ and farewell. Simon Smith BSc MIBMS Chief Technical Officer Histion LLC 2615 W Casino Rd Unit 6G Everett, WA 98204 smiths@Histion.com T: (206) 953 1386 F: (425) 353-3604 LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorised. If you are not an adressee, any disclosure or copying of the contents of this e-mail or any action taken (or not taken) in reliance on it is unauthorised and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Nov 12 17:41:19 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Nov 12 17:41:25 2009 Subject: [Histonet] RE: Akemi - Response to Vinnie In-Reply-To: Message-ID: <872670.66527.qm@web113803.mail.gq1.yahoo.com> Hi Vinnie and anyone else who is in my address book, I am so sorry! ?This has become a nightmare. ?I have tried to contact everyone, but I must have at least close to 300 people in my address book. Akemi --- On Thu, 11/12/09, Della Speranza, Vinnie wrote: From: Della Speranza, Vinnie Subject: [Histonet] RE: Akemi - Urgent! To: "'Helen Fedor'" , "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" Date: Thursday, November 12, 2009, 3:30 PM I also received the scam message purportedly from Akemi so something is amiss. I can only assume Akemi that your address book/contact list has been acquired by whoever is behind this. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 12, 2009 9:30 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Akemi - Urgent! I also receive one of the scam emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please.? I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Thu Nov 12 18:08:07 2009 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Thu Nov 12 18:08:11 2009 Subject: [Histonet] FW: RIP Russ Allison References: <1AAF670737F193429070841C6B2ADD4CF76A2DB8@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <2108AECB05DBFF48A9C436A792155740010C3794@UWHC-MAIL03.uwhis.hosp.wisc.edu> I had the great privilege to speak with Russ Allison at a conference in Australia several years ago. He was a funny man, he was a wild man and enjoyable to be around. His birthday was October 31st - and a Halloween has never gone by since or will go by that I won't think of him. Cheers Russ!!!! Jean Mitchell UWHC- Neuromuscular Laboratory Madison, WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim Sent: Thu 11/12/2009 5:36 PM To: Hotmail; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: RIP Russ Allison I'm sorry to hear that. I met Russ several times at NSH over the years and had several email correspondences with him. He was always helpful and a great guy. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hotmail Sent: Thursday, November 12, 2009 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: RIP Russ Allison Vintage histonetters will remember the valuable contributions to our body of knowledge made by Russ Allison. I was saddened to hear of his recent passing after a seven year battle with Alzheimers. I'll not presume to eulogize him, there are many who knew him better and longer, suffice it to say you would have to go a long way to find a kinder, more helpful man with as great a passion for laboratory science, scientific education and Welsh rugby. On his annual trips to the US to present workshops at NSH meetings he would always find time for a pint or two and spirited discussion about the progress of the profession on both sides of the pond. The following is the URL of an obituary of Russ by the current chief executive if the Institute of Biomedical Scientists. http://www.ibms.org/go/media-centre:monthly-comment I'm off to the beer aisle so I can lift a Brains in his honour tonight. Cheers, Russ and farewell. Simon Smith BSc MIBMS Chief Technical Officer Histion LLC 2615 W Casino Rd Unit 6G Everett, WA 98204 smiths@Histion.com T: (206) 953 1386 F: (425) 353-3604 LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorised. If you are not an adressee, any disclosure or copying of the contents of this e-mail or any action taken (or not taken) in reliance on it is unauthorised and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ndevans <@t> stanford.edu Thu Nov 12 19:19:54 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Thu Nov 12 19:19:58 2009 Subject: [Histonet] CD31/PECAM antibody Message-ID: Hello, Can anyone suggest a good antibody to recognize mouse CD31 (PECAM)? I have been trying to use BD Pharmingen MEC 13.3 with little success. I use vector lab's goat anti-rat (BA-9400) but get loads of background on mouse sections with this secondary. I also tried a mouse-on-mouse kit and saw much less background, but still no signal. I'd be very grateful for advice. Best wishes Nick From tifei <@t> foxmail.com Thu Nov 12 20:10:31 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Nov 12 20:41:06 2009 Subject: [Histonet] CD31/PECAM antibody References: Message-ID: <200911131010299847277@foxmail.com> aGkuLi50aGF0IG9uZSB3b3Jrcy4uLmFjY29yZGluZyB0byBteSBjb2xsZWd1ZQ0KQlVULi4uZG9u dCB1c2UgUEZBIGZpeGF0aW9uDQp1IGNhbiBkbyBmcm96ZW4gc2VjdGlvbiwgb3IgcGFycmFmaW4g c2VjdGlvbiB3aXRoIFppbmMgZml4YXRpb24NCg0KDQoNCjIwMDktMTEtMTMgDQoNCg0KDQpURiAN Cg0KDQoNCreivP7Iy6O6IE5pY2hvbGFzIERhdmlkIEV2YW5zIA0Kt6LLzcqxvOSjuiAyMDA5LTEx LTEzICAwOToyNDowOSANCsrVvP7Iy6O6IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVk dSANCrOty82juiANCtb3zOKjuiBbSGlzdG9uZXRdIENEMzEvUEVDQU0gYW50aWJvZHkgDQogDQpI ZWxsbywNCg0KQ2FuIGFueW9uZSBzdWdnZXN0IGEgZ29vZCBhbnRpYm9keSB0byByZWNvZ25pemUg bW91c2UgQ0QzMSAoUEVDQU0pPyBJIGhhdmUNCmJlZW4gdHJ5aW5nIHRvIHVzZSBCRCBQaGFybWlu Z2VuIE1FQyAxMy4zIHdpdGggbGl0dGxlIHN1Y2Nlc3MuIEkgdXNlDQp2ZWN0b3IgbGFiJ3MgZ29h dCBhbnRpLXJhdCAoQkEtOTQwMCkgYnV0IGdldCBsb2FkcyBvZiBiYWNrZ3JvdW5kIG9uIG1vdXNl DQpzZWN0aW9ucyB3aXRoIHRoaXMgc2Vjb25kYXJ5LiBJIGFsc28gdHJpZWQgYSBtb3VzZS1vbi1t b3VzZSBraXQgYW5kIHNhdw0KbXVjaCBsZXNzIGJhY2tncm91bmQsIGJ1dCBzdGlsbCBubyBzaWdu YWwuIEknZCBiZSB2ZXJ5IGdyYXRlZnVsIGZvcg0KYWR2aWNlLg0KDQpCZXN0IHdpc2hlcw0KTmlj aw0KX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkhpc3Rv bmV0IG1haWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRw Oi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQNCg== From melanie.black <@t> uct.ac.za Fri Nov 13 02:00:44 2009 From: melanie.black <@t> uct.ac.za (Melanie Black) Date: Fri Nov 13 02:02:40 2009 Subject: [Histonet] Re: CD31 In-Reply-To: <35069128C2074041A49371F8131E548883AEB9@ITSSSXM01V6.one.ads.che.org> References: <35069128C2074041A49371F8131E548883AEB9@ITSSSXM01V6.one.ads.che.org> Message-ID: <4B6C2513-DFD7-459E-9ABC-CC4C037A9933@uct.ac.za> Hi Cindy We found that the coating for the slides interfered with staining, and it took many hours to work that out!! If you have good staining using coated slides, no problem. Why not try one of each? Regards Melanie On 12 Nov 2009, at 6:06 PM, Baranowski Cynthia wrote: > Melanie, > I saw your posting on Histonet in response to CD31 on zinc fixed > rat tissue. You stated that the sections must be mounted onto > uncoated slides. Why is that necessary? > I will be attempting to work up this antibody on zinc-fixed rat > tissue embedded in methyl methacrylate. > Regards, > Cindy Baranowski > Senior Histologist > Plastic Histology > > SJTRI > 380-B Northyards Blvd. > Atlanta, GA 30313 > (678) 843-6512 : Direct > (678) 843-6500 : Facsimile > cbaranowski@sjha.org : E-mail > > > > NOTICE: This e-mail message and all attachments transmitted with it > may contain legally privileged and confidential information > intended solely for the use of the addressee. In addition, this > correspondence may contain private patient information protected > under the federal privacy rule, 45 C.F.R. Parts 160 and 164, and > applicable state law. Unauthorized use or disclosure of this > information is strictly prohibited. If the reader of this message > is not the intended recipient, you are hereby notified that any > reading, dissemination, distribution, copying or other use of this > message or its attachments is strictly prohibited. If you have > received this message in error, please notify the sender > immediately by return e-mail or at the telephone number above and > delete the original message and all copies and backups thereof. > Thank you. > > > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. Melanie Black 082 469 3352 Cardiovascular Research Unit 3rd Floor; Chris Barnard Building Medical School; Observatory. 7925. University of Cape Town. South Africa. From c.m.vanderloos <@t> amc.uva.nl Fri Nov 13 05:18:57 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Nov 13 05:19:15 2009 Subject: [Histonet] RE: Looking for VEGF and FGF-2 antibodies Message-ID: Hi Paul,We just completed a study into different VEGF-A antibodies. Our paper is excepted for J Histochem Cytochem (www.jhc.org) and is available as PDF under exPRESS at September 28th. Thermo/Labvision RB-9031 came out as best antibody.Cheers,Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 12 Nov 2009 10:26:48 -0500 From: "Monfils, Paul" Subject: [Histonet] Looking for VEGF and FGF-2 antibodies To: I am having a difficult time finding two antibodies I need. Two companies I ordered them from have discontinued them. VEGF and FGF-2 The tissue is human. Monoclonals preferred but at this point I'll use polyclonals if necessary. Does anyone know a vendor who has them? Thanks. From Carol.Wilson <@t> ricerca.com Fri Nov 13 06:40:48 2009 From: Carol.Wilson <@t> ricerca.com (Wilson, Carol) Date: Fri Nov 13 06:40:56 2009 Subject: [Histonet] Muscle injection site in rats Message-ID: <9D443EB9D0270143B5AAF190CB1A58A309747080@dogwood.ricerca.com> Hi All, Is there a way to isolate the point of injection for histology on an intramuscular injection into a rat thigh? Any suggestions or protocols would be appreciated. Thanks, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology From DKnutson <@t> primecare.org Fri Nov 13 08:43:21 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Fri Nov 13 08:44:04 2009 Subject: [Histonet] B5 alternate Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B68BF@exchangent> Am looking for advice on what alternate fixative to use to replace B5. What do the majority of you histonetters use? Does it work well with immunos? The pros and cons? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From 41dmb41 <@t> gmail.com Fri Nov 13 08:53:11 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Fri Nov 13 08:53:35 2009 Subject: [Histonet] B5 alternate In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B68BF@exchangent> References: <4F0B7161A6CD524FAD8017D52E1553400D2B68BF@exchangent> Message-ID: In my lab, we're using B-Plus Fixative by BBC Biochemical. I know many other labs that are also using it as their B5 alternative. The biggest pro, of course, is that it's mercury free and not hazardous. Also, it's not as sensitive to over-fixation like B5 is. The guideline I use is 4 hours minimum fixation for Bone Marrows and Lymphoid tissue. After that, routine formalin processing is all you need to do. You can, however, leave the specimen in B-Plus for up to 48 hours without adversely effecting the tissue sample. Hope this help. Drew Meyer On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne wrote: > Am looking for advice on what alternate fixative to use to replace B5. ?What > do the majority of you histonetters use? ?Does it work well with immunos? > The pros and cons? ?Thank you for your recommendations. > > > > Deanne Knutson > > Anatomic Pathology Supervisor > > St. Alexius Medical Center > > 900 E. Broadway > > Bismarck, North Dakota ?58506 > > (701)-530-6730 > > dknutson@primecare.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From annigyg <@t> gmail.com Fri Nov 13 09:00:23 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Nov 13 09:00:28 2009 Subject: [Histonet] B5 alternate In-Reply-To: References: <4F0B7161A6CD524FAD8017D52E1553400D2B68BF@exchangent> Message-ID: B plus is the one - we use it as stated and it works like a charm immunos are good too - we are proudly mercury free Annie 2009/11/13 Drew Meyer <41dmb41@gmail.com> > In my lab, we're using B-Plus Fixative by BBC Biochemical. I know > many other labs that are also using it as their B5 alternative. The > biggest pro, of course, is that it's mercury free and not hazardous. > Also, it's not as sensitive to over-fixation like B5 is. The > guideline I use is 4 hours minimum fixation for Bone Marrows and > Lymphoid tissue. After that, routine formalin processing is all you > need to do. You can, however, leave the specimen in B-Plus for up to > 48 hours without adversely effecting the tissue sample. > > Hope this help. > > Drew Meyer > > On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne > wrote: > > Am looking for advice on what alternate fixative to use to replace B5. > What > > do the majority of you histonetters use? Does it work well with immunos? > > The pros and cons? Thank you for your recommendations. > > > > > > > > Deanne Knutson > > > > Anatomic Pathology Supervisor > > > > St. Alexius Medical Center > > > > 900 E. Broadway > > > > Bismarck, North Dakota 58506 > > > > (701)-530-6730 > > > > dknutson@primecare.org > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From leiker <@t> buffalo.edu Fri Nov 13 09:06:20 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Nov 13 09:06:25 2009 Subject: [Histonet] Muscle injection site in rats In-Reply-To: <9D443EB9D0270143B5AAF190CB1A58A309747080@dogwood.ricerca.com> References: <9D443EB9D0270143B5AAF190CB1A58A309747080@dogwood.ricerca.com> Message-ID: <77681A9F79D8824DB2E0EBB7@CDYwxp1931.ad.med.buffalo.edu> We've had this problem before! The best we could do was to shave the area we wanted to inject, then used an ink tattooing device our animal facilities had which basically dipped a needle into a permanent nontoxic bright green dye that you then punch the skin with right where you want to inject. Do it the day before you inject, so the excess dye has time to come off. The next day you see the little dye mark in the puncture site and that's your bulls' eye for where you do your injection. It will stay for weeks at least. Seemed to work for our purposes! We did it with hamsters and it was a 2 person job: one to hold the animal while punching/injecting, the other to pull the leg straight by the foot. Or the one to hold the animal with the other holding the leg and punching/injecting. (Sometimes we tried using a Sharpie to circle the site on the shaved skin so we could locate it easier weeks down the road, but then you have to re-circle it every day because it wears of quickly with their grooming and etc.) Maybe there are other, better methods out there...it would be great to hear them!! Regards, Merced --On Friday, November 13, 2009 7:40 AM -0500 "Wilson, Carol" wrote: > Hi All, > > Is there a way to isolate the point of injection for histology on an > intramuscular injection into a rat thigh? Any suggestions or protocols > would be appreciated. > > Thanks, > > Carol > > > > Carol Wilson, HT(ASCP) > > Lead Technician/Histology > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From MMorin <@t> Lifespan.org Fri Nov 13 09:14:13 2009 From: MMorin <@t> Lifespan.org (Morin, Mary Anne) Date: Fri Nov 13 09:14:19 2009 Subject: [Histonet] B5 alternate In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B68BF@exchangent> Message-ID: <130E8991F210424096EFC6F42EA33B2463955F@LSCOEXCH1.lsmaster.lifespan.org> Hi, we replaced B5 with B-Plus Fixative, we use it for our bone marrow bx's and aspirates. They seem to be happy with the results, many IHC's are ordered on the bone marrows< Haven't heard any complaints. I order it from BBC Biochemical Catalog # 1751 for 1 gallon phone # is 1-800-635-4477. Have A Good One!! Marie Anne Morin H.T. ASCP Path Tech Specialist Histology Lab Rhode Island Hospital Office 444-7196 Pager 350-7384 Fax 444-8514 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Knutson, Deanne Sent: Friday, November 13, 2009 9:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] B5 alternate Am looking for advice on what alternate fixative to use to replace B5. What do the majority of you histonetters use? Does it work well with immunos? The pros and cons? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Nov 13 09:16:32 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 13 09:16:46 2009 Subject: [Histonet] B5 alternate In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B68BF@exchangent> Message-ID: <921384.74040.qm@web65712.mail.ac4.yahoo.com> B5 is an inheritance of the "toxic good old days". It is really a good fixative, but after thorough testing, my hematologist (a very talented but picky pathologist) agreed that the ubiquitous yethumble?Neutral Buffered Formalin produced equivalent results. There is one caveat though: the pH HAS to be EXACTLY 7 and the washings to be done also with phosphate buffer at pH7. Other than than you can eliminate the mercury. Ren? J. --- On Fri, 11/13/09, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] B5 alternate To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, November 13, 2009, 9:43 AM Am looking for advice on what alternate fixative to use to replace B5.? What do the majority of you histonetters use?? Does it work well with immunos? The pros and cons?? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota? 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Nov 13 09:33:33 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Nov 13 09:33:39 2009 Subject: [Histonet] "Email Spoofed." addresses were highjacked Message-ID: <529402.12738.qm@web113818.mail.gq1.yahoo.com> Hi All, The email below was sent to me in response to the Scam. ?I guess someone from histonet contacted him regarding the scam, which was spammed. ?It's interesting to find out how vulnerable we all are. ?Yahoo's customer support has had high volumes of similar problems. Akemi, Boy!? Peter needs an Oxycontin!? Maybe more than one. Don't worry about this.? The correct term is being "Email Spoofed."? From my friends, it happens when some computer hijacker either creates content on a new or existing web site that you ended up clicking on.? Peter suggests it is "Where" you visited, but it could be anywhere, even on a legitimate site.? Some people I've talked to think Microsoft's web page is loaded with "Spoof" and "Spyware!" Sometimes, it is a computer worm or virus affecting your computer.? Most of the time, it is associated with your email provider.? [Most of my friends with?Yahoo?email have been "Spoofed."]?? I would run a up-to-date anti-virus software, and (especially if that doesn't help) ask for help from Yahoo to quarantine or fix your address.?? You probably have it fixed already.? If not, it will do it again until someone stops it.? It IS only email.? So what??? Hugh-Hawaii From S_T_E_F_F_I <@t> gmx.net Fri Nov 13 09:46:12 2009 From: S_T_E_F_F_I <@t> gmx.net (Steffi Linnerbauer) Date: Fri Nov 13 09:46:22 2009 Subject: [Histonet] gp350 Message-ID: <20091113154612.308820@gmx.net> Dear all, I try to establish an EBV gp350 staining on human EBV positive tumors but don?t get a result until now. I use a hybridoma supernatant as antibody and don?t get a result in my positive control. Is there anyone who have already experience with gp350 antibodies on FFPF or cryo tissues? What are good antibodies? any special tipps for the staining? thanks in advance -- Jetzt kostenlos herunterladen: Internet Explorer 8 und Mozilla Firefox 3.5 - sicherer, schneller und einfacher! http://portal.gmx.net/de/go/chbrowser From carrolpb <@t> umdnj.edu Fri Nov 13 09:52:21 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Nov 13 09:52:33 2009 Subject: [Histonet] "Email Spoofed." addresses were highjacked In-Reply-To: <529402.12738.qm@web113818.mail.gq1.yahoo.com> References: <529402.12738.qm@web113818.mail.gq1.yahoo.com> Message-ID: <4AFD80B5.1070506@umdnj.edu> > Boy! Peter needs an Oxycontin! Maybe more than one. Since I've already apparently made the impression of being up tight, I may as well go on and also add that, on top of the ~15 off-topic emails we've already gotten concerning an email scam you fell for, I sort of feel that sending someone else's privately-mailed insult of a perfect stranger to an entire mailing list to be a bit, well, tactless. Oh well, at least its friday, eh? :) Akemi Allison-Tacha wrote: > Hi All, > > The email below was sent to me in response to the Scam. I guess someone from histonet contacted him regarding the scam, which was spammed. It's interesting to find out how vulnerable we all are. Yahoo's customer support has had high volumes of similar problems. > > Akemi, > > Boy! Peter needs an Oxycontin! Maybe more than > one. > > Don't worry about this. The correct term is being "Email Spoofed." From my friends, it happens when some computer hijacker either creates content on a new or existing web site that you ended up clicking on. Peter suggests it is "Where" you visited, but it could be anywhere, even on a legitimate site. Some people I've talked to think Microsoft's web page is loaded with "Spoof" and "Spyware!" > > Sometimes, it is a computer worm or virus affecting your computer. Most of the time, it is associated with your email > provider. [Most of my friends with Yahoo email have been "Spoofed."] > > I would run a up-to-date anti-virus software, and (especially if that doesn't help) ask for help from Yahoo to quarantine or fix your address. > > You probably have it fixed already. If > not, it will do it again until someone stops it. It IS only email. So what??? > > Hugh-Hawaii > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Hooten <@t> medinst.com Fri Nov 13 10:11:27 2009 From: Hooten <@t> medinst.com (Scott Hooten) Date: Fri Nov 13 10:11:35 2009 Subject: [Histonet] Staining Spurrs Message-ID: <4AFD3EDF.8FD5.00E5.0@medinst.com> Hello All, I recently began working with Spurr's Resin and was working on doing an H&E stain. The H&E stain I use for my MMA does not have the best results on Spurr's and was wondering if anyone has an H&E staining technique for Spurr's that they could share with me. Any help is greatly appreciated. Thanks. Scott R. Hooten Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-464-0817 ext. 1115 From pruegg <@t> ihctech.net Fri Nov 13 10:20:23 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 13 10:20:59 2009 Subject: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2 antibodies In-Reply-To: References: Message-ID: <3269D2B1748C43AAA7AC8383F79B2F8C@Patsyoffice> Check out www.peprotech.com for these antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Friday, November 13, 2009 4:19 AM To: histonet@lists.utsouthwestern.edu Cc: PMonfils@Lifespan.org Subject: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2 antibodies Hi Paul,We just completed a study into different VEGF-A antibodies. Our paper is excepted for J Histochem Cytochem (www.jhc.org) and is available as PDF under exPRESS at September 28th. Thermo/Labvision RB-9031 came out as best antibody.Cheers,Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 12 Nov 2009 10:26:48 -0500 From: "Monfils, Paul" Subject: [Histonet] Looking for VEGF and FGF-2 antibodies To: I am having a difficult time finding two antibodies I need. Two companies I ordered them from have discontinued them. VEGF and FGF-2 The tissue is human. Monoclonals preferred but at this point I'll use polyclonals if necessary. Does anyone know a vendor who has them? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmarti <@t> cmrb.eu Fri Nov 13 11:49:43 2009 From: mmarti <@t> cmrb.eu (Marti Gaudes, Merce) Date: Fri Nov 13 12:21:00 2009 Subject: [Histonet] Ki67 Ab and specific antigen retrieval Message-ID: Hi. I need to detect Ki67 in paraffin Mouse samples. Please, does any of you know a good Ab, and if I need any antigen retrieval with this antigen? Thanks a lot for your help. Merc? Mart? Gaudes mmarti@cmrb.eu -------- Abans d'imprimir aquest missatge, si us plau, comprova que ??s realment necessari. El medi ambient ??s cosa de tots. Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. -------- La informaci?? continguda en aquest missatge i en qualsevol fitxer adjunt ??s confidencial, privada i d'??s exclusiu per al destinatari. Si no ??s la persona a la qual anava dirigida aquesta informaci??, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c??pia, divulgaci??, distribuci?? o utilitzaci?? no autoritzada d'aquest correu electr??nic i dels seus adjunts est?? prohibida en virtut de la legislaci?? vigent. La informaci??n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci??n, por favor, notifique inmediatamente el env??o err??neo al remitente y borre el mensaje. Cualquier copia, divulgaci??n, distribuci??n o utilizaci??n no autorizada de este correo electr??nico y de sus adjuntos est?? prohibida en virtud de la legislaci??n vigente. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. -------- From vgrover <@t> polysciences.com Fri Nov 13 12:24:17 2009 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Fri Nov 13 12:24:40 2009 Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 14 Message-ID: <223879AF22884F89B837204A709577D8@USWARD13ZFB71> Oh beyond fun! We'll have more fun to come. Did you make it obvious? Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 72, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: B5 alternate (Drew Meyer) 2. Re: B5 alternate (Anne van Binsbergen) 3. Re: Muscle injection site in rats (Merced M Leiker) 4. RE: B5 alternate (Morin, Mary Anne) 5. Re: B5 alternate (Rene J Buesa) 6. "Email Spoofed." addresses were highjacked (Akemi Allison-Tacha) 7. gp350 (Steffi Linnerbauer) 8. Re: "Email Spoofed." addresses were highjacked (Peter Carroll) 9. Staining Spurrs (Scott Hooten) 10. RE: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2 antibodies (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 13 Nov 2009 09:53:11 -0500 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] B5 alternate To: "Knutson, Deanne" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 In my lab, we're using B-Plus Fixative by BBC Biochemical. I know many other labs that are also using it as their B5 alternative. The biggest pro, of course, is that it's mercury free and not hazardous. Also, it's not as sensitive to over-fixation like B5 is. The guideline I use is 4 hours minimum fixation for Bone Marrows and Lymphoid tissue. After that, routine formalin processing is all you need to do. You can, however, leave the specimen in B-Plus for up to 48 hours without adversely effecting the tissue sample. Hope this help. Drew Meyer On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne wrote: > Am looking for advice on what alternate fixative to use to replace B5. What > do the majority of you histonetters use? Does it work well with immunos? > The pros and cons? Thank you for your recommendations. > > > > Deanne Knutson > > Anatomic Pathology Supervisor > > St. Alexius Medical Center > > 900 E. Broadway > > Bismarck, North Dakota 58506 > > (701)-530-6730 > > dknutson@primecare.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Fri, 13 Nov 2009 19:00:23 +0400 From: Anne van Binsbergen Subject: Re: [Histonet] B5 alternate To: Drew Meyer <41dmb41@gmail.com> Cc: "Knutson, Deanne" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 B plus is the one - we use it as stated and it works like a charm immunos are good too - we are proudly mercury free Annie 2009/11/13 Drew Meyer <41dmb41@gmail.com> > In my lab, we're using B-Plus Fixative by BBC Biochemical. I know > many other labs that are also using it as their B5 alternative. The > biggest pro, of course, is that it's mercury free and not hazardous. > Also, it's not as sensitive to over-fixation like B5 is. The > guideline I use is 4 hours minimum fixation for Bone Marrows and > Lymphoid tissue. After that, routine formalin processing is all you > need to do. You can, however, leave the specimen in B-Plus for up to > 48 hours without adversely effecting the tissue sample. > > Hope this help. > > Drew Meyer > > On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne > wrote: > > Am looking for advice on what alternate fixative to use to replace B5. > What > > do the majority of you histonetters use? Does it work well with immunos? > > The pros and cons? Thank you for your recommendations. > > > > > > > > Deanne Knutson > > > > Anatomic Pathology Supervisor > > > > St. Alexius Medical Center > > > > 900 E. Broadway > > > > Bismarck, North Dakota 58506 > > > > (701)-530-6730 > > > > dknutson@primecare.org > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 3 Date: Fri, 13 Nov 2009 10:06:20 -0500 From: Merced M Leiker Subject: Re: [Histonet] Muscle injection site in rats To: "Wilson, Carol" , histonet@lists.utsouthwestern.edu Message-ID: <77681A9F79D8824DB2E0EBB7@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed We've had this problem before! The best we could do was to shave the area we wanted to inject, then used an ink tattooing device our animal facilities had which basically dipped a needle into a permanent nontoxic bright green dye that you then punch the skin with right where you want to inject. Do it the day before you inject, so the excess dye has time to come off. The next day you see the little dye mark in the puncture site and that's your bulls' eye for where you do your injection. It will stay for weeks at least. Seemed to work for our purposes! We did it with hamsters and it was a 2 person job: one to hold the animal while punching/injecting, the other to pull the leg straight by the foot. Or the one to hold the animal with the other holding the leg and punching/injecting. (Sometimes we tried using a Sharpie to circle the site on the shaved skin so we could locate it easier weeks down the road, but then you have to re-circle it every day because it wears of quickly with their grooming and etc.) Maybe there are other, better methods out there...it would be great to hear them!! Regards, Merced --On Friday, November 13, 2009 7:40 AM -0500 "Wilson, Carol" wrote: > Hi All, > > Is there a way to isolate the point of injection for histology on an > intramuscular injection into a rat thigh? Any suggestions or protocols > would be appreciated. > > Thanks, > > Carol > > > > Carol Wilson, HT(ASCP) > > Lead Technician/Histology > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 4 Date: Fri, 13 Nov 2009 10:14:13 -0500 From: "Morin, Mary Anne" Subject: RE: [Histonet] B5 alternate To: "Knutson, Deanne" , Message-ID: <130E8991F210424096EFC6F42EA33B2463955F@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Hi, we replaced B5 with B-Plus Fixative, we use it for our bone marrow bx's and aspirates. They seem to be happy with the results, many IHC's are ordered on the bone marrows< Haven't heard any complaints. I order it from BBC Biochemical Catalog # 1751 for 1 gallon phone # is 1-800-635-4477. Have A Good One!! Marie Anne Morin H.T. ASCP Path Tech Specialist Histology Lab Rhode Island Hospital Office 444-7196 Pager 350-7384 Fax 444-8514 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Knutson, Deanne Sent: Friday, November 13, 2009 9:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] B5 alternate Am looking for advice on what alternate fixative to use to replace B5. What do the majority of you histonetters use? Does it work well with immunos? The pros and cons? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 13 Nov 2009 07:16:32 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] B5 alternate To: "'histonet@lists.utsouthwestern.edu'" , DeanneKnutson Message-ID: <921384.74040.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 B5 is an inheritance of the "toxic good old days". It is really a good fixative, but after thorough testing, my hematologist (a very talented but picky pathologist) agreed that the ubiquitous yethumble Neutral Buffered Formalin produced equivalent results. There is one caveat though: the pH HAS to be EXACTLY 7 and the washings to be done also with phosphate buffer at pH7. Other than than you can eliminate the mercury. Reni J. --- On Fri, 11/13/09, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] B5 alternate To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, November 13, 2009, 9:43 AM Am looking for advice on what alternate fixative to use to replace B5. What do the majority of you histonetters use? Does it work well with immunos? The pros and cons? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 13 Nov 2009 07:33:33 -0800 (PST) From: Akemi Allison-Tacha Subject: [Histonet] "Email Spoofed." addresses were highjacked To: histo net Message-ID: <529402.12738.qm@web113818.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, The email below was sent to me in response to the Scam. I guess someone from histonet contacted him regarding the scam, which was spammed. It's interesting to find out how vulnerable we all are. Yahoo's customer support has had high volumes of similar problems. Akemi, Boy! Peter needs an Oxycontin! Maybe more than one. Don't worry about this. The correct term is being "Email Spoofed." From my friends, it happens when some computer hijacker either creates content on a new or existing web site that you ended up clicking on. Peter suggests it is "Where" you visited, but it could be anywhere, even on a legitimate site. Some people I've talked to think Microsoft's web page is loaded with "Spoof" and "Spyware!" Sometimes, it is a computer worm or virus affecting your computer. Most of the time, it is associated with your email provider. [Most of my friends with Yahoo email have been "Spoofed."] I would run a up-to-date anti-virus software, and (especially if that doesn't help) ask for help from Yahoo to quarantine or fix your address. You probably have it fixed already. If not, it will do it again until someone stops it. It IS only email. So what??? Hugh-Hawaii ------------------------------ Message: 7 Date: Fri, 13 Nov 2009 16:46:12 +0100 From: "Steffi Linnerbauer" Subject: [Histonet] gp350 To: histonet@lists.utsouthwestern.edu Message-ID: <20091113154612.308820@gmx.net> Content-Type: text/plain; charset="iso-8859-1" Dear all, I try to establish an EBV gp350 staining on human EBV positive tumors but don4t get a result until now. I use a hybridoma supernatant as antibody and don4t get a result in my positive control. Is there anyone who have already experience with gp350 antibodies on FFPF or cryo tissues? What are good antibodies? any special tipps for the staining? thanks in advance -- Jetzt kostenlos herunterladen: Internet Explorer 8 und Mozilla Firefox 3.5 - sicherer, schneller und einfacher! http://portal.gmx.net/de/go/chbrowser ------------------------------ Message: 8 Date: Fri, 13 Nov 2009 10:52:21 -0500 From: Peter Carroll Subject: Re: [Histonet] "Email Spoofed." addresses were highjacked To: Akemi Allison-Tacha Cc: histo net Message-ID: <4AFD80B5.1070506@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > Boy! Peter needs an Oxycontin! Maybe more than one. Since I've already apparently made the impression of being up tight, I may as well go on and also add that, on top of the ~15 off-topic emails we've already gotten concerning an email scam you fell for, I sort of feel that sending someone else's privately-mailed insult of a perfect stranger to an entire mailing list to be a bit, well, tactless. Oh well, at least its friday, eh? :) Akemi Allison-Tacha wrote: > Hi All, > > The email below was sent to me in response to the Scam. I guess someone from histonet contacted him regarding the scam, which was spammed. It's interesting to find out how vulnerable we all are. Yahoo's customer support has had high volumes of similar problems. > > Akemi, > > Boy! Peter needs an Oxycontin! Maybe more than > one. > > Don't worry about this. The correct term is being "Email Spoofed." From my friends, it happens when some computer hijacker either creates content on a new or existing web site that you ended up clicking on. Peter suggests it is "Where" you visited, but it could be anywhere, even on a legitimate site. Some people I've talked to think Microsoft's web page is loaded with "Spoof" and "Spyware!" > > Sometimes, it is a computer worm or virus affecting your computer. Most of the time, it is associated with your email > provider. [Most of my friends with Yahoo email have been "Spoofed."] > > I would run a up-to-date anti-virus software, and (especially if that doesn't help) ask for help from Yahoo to quarantine or fix your address. > > You probably have it fixed already. If > not, it will do it again until someone stops it. It IS only email. So what??? > > Hugh-Hawaii > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 9 Date: Fri, 13 Nov 2009 11:11:27 -0500 From: "Scott Hooten" Subject: [Histonet] Staining Spurrs To: Message-ID: <4AFD3EDF.8FD5.00E5.0@medinst.com> Content-Type: text/plain; charset=US-ASCII Hello All, I recently began working with Spurr's Resin and was working on doing an H&E stain. The H&E stain I use for my MMA does not have the best results on Spurr's and was wondering if anyone has an H&E staining technique for Spurr's that they could share with me. Any help is greatly appreciated. Thanks. Scott R. Hooten Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-464-0817 ext. 1115 ------------------------------ Message: 10 Date: Fri, 13 Nov 2009 09:20:23 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2 antibodies To: "'C.M. van der Loos'" , Cc: PMonfils@Lifespan.org Message-ID: <3269D2B1748C43AAA7AC8383F79B2F8C@Patsyoffice> Content-Type: text/plain; charset="us-ascii" Check out www.peprotech.com for these antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Friday, November 13, 2009 4:19 AM To: histonet@lists.utsouthwestern.edu Cc: PMonfils@Lifespan.org Subject: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2 antibodies Hi Paul,We just completed a study into different VEGF-A antibodies. Our paper is excepted for J Histochem Cytochem (www.jhc.org) and is available as PDF under exPRESS at September 28th. Thermo/Labvision RB-9031 came out as best antibody.Cheers,Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 12 Nov 2009 10:26:48 -0500 From: "Monfils, Paul" Subject: [Histonet] Looking for VEGF and FGF-2 antibodies To: I am having a difficult time finding two antibodies I need. Two companies I ordered them from have discontinued them. VEGF and FGF-2 The tissue is human. Monoclonals preferred but at this point I'll use polyclonals if necessary. Does anyone know a vendor who has them? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 14 **************************************** From ndevans <@t> stanford.edu Fri Nov 13 13:36:40 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Fri Nov 13 13:36:44 2009 Subject: [Histonet] CD31/PECAM antibody In-Reply-To: References: Message-ID: <64590973E2D94ACCA9E8C4A095C76D8B@DellDesktop2> Thanks all for advice. Biocare's Rat on mouse Ig detection kit seems to be the popular choice and I'm going to have a go with this before writing of the BD Pharmingen antibody. Best wishes Nick -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, November 12, 2009 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31/PECAM antibody Hello, Can anyone suggest a good antibody to recognize mouse CD31 (PECAM)? I have been trying to use BD Pharmingen MEC 13.3 with little success. I use vector lab's goat anti-rat (BA-9400) but get loads of background on mouse sections with this secondary. I also tried a mouse-on-mouse kit and saw much less background, but still no signal. I'd be very grateful for advice. Best wishes Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 13 14:37:59 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 13 14:38:24 2009 Subject: [Histonet] amnion tissue Message-ID: <08E33DE0F80D4039AE7BC40A16299628@Patsyoffice> Has anyone ever done histology (ffpe or frozen) on human amnion tissue? I have an investigator that wants to send me 2X3in pieces of amnion tissue for histology to evaluate changes in structural collagen? Not sure of how I would make a histology section out of something like that??? I imagine it is a thin piece of membrane? Help, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From contact <@t> excaliburpathology.com Fri Nov 13 14:49:45 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Nov 13 14:49:49 2009 Subject: [Histonet] amnion tissue In-Reply-To: <08E33DE0F80D4039AE7BC40A16299628@Patsyoffice> References: <08E33DE0F80D4039AE7BC40A16299628@Patsyoffice> Message-ID: <332149.11078.qm@web1107.biz.mail.sk1.yahoo.com> Hi Patsy, roll it up like you would cinnamon roll dough and cut through the layers. Take care to place the cross-section in the cassette so it stays rolled during processing. Paula? ________________________________ From: Patsy Ruegg To: histonet@lists.utsouthwestern.edu Sent: Fri, November 13, 2009 2:37:59 PM Subject: [Histonet] amnion tissue Has anyone ever done histology (ffpe or frozen) on human amnion tissue?? I have an investigator that wants to send me 2X3in pieces of amnion tissue for histology to evaluate changes in structural collagen?? Not sure of how I would make a histology section out of something like that???? I imagine it is a thin piece of membrane? Help, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Fri Nov 13 15:04:20 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Nov 13 15:12:35 2009 Subject: [Histonet] Re: B5 alternate Message-ID: I loved B5 fixative, and before that Zenker's, but they're gone and they're not coming back. In my personal expereience in a number of pathology services, the various muchly-touted B5 substitutes offer very little morphologic advantage over neutral buffered formalin. Unless it's fixed pretty early in the morning, tissue needs to sit overnight in neutral buffered formalin before being decalcified or processed. Pathologists and their clinicians are eventually going to have to learn to live with this delay. Bob Richmond Samurai Pathologist Knoxville TN From PMonfils <@t> Lifespan.org Fri Nov 13 17:24:44 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Nov 13 17:24:59 2009 Subject: [Histonet] amnion tissue In-Reply-To: <08E33DE0F80D4039AE7BC40A16299628@Patsyoffice> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835D07@LSRIEXCH1.lsmaster.lifespan.org> I fold the membrane back and forth on a wet piece of lens paper in a petrie dish. This gives me an elongate mass several layers thick and about 1.5 cm in width. I wrap this in lens paper and insert it into a nylon biopsy bag for processing. This holds the whole thing flat. For embedding I unwrap the specimen (which now adheres together pretty well), slice the whole thing right down the center with a scalpel blade, and embed both halves with the cut edge down, right next to each other in the same block. This provides a lot of material to look at microscopically, a dozen or more full length cross sections of the membrane. From jkiernan <@t> uwo.ca Fri Nov 13 23:15:00 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Nov 13 23:15:08 2009 Subject: [Histonet] FW: RIP Russ Allison In-Reply-To: <2108AECB05DBFF48A9C436A792155740010C3794@UWHC-MAIL03.uwhis.hosp.wisc.edu> References: <1AAF670737F193429070841C6B2ADD4CF76A2DB8@EXMBMCB15.ucsfmedicalcenter.org> <2108AECB05DBFF48A9C436A792155740010C3794@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: I don't think Russ can have had a "battle with Alzheimer's for 7 years". He was in good mental condition in Nov 2001, as Jean Mitchell can also attest. (I was at the same conference.) According to his IBMS obituary, http://www.ibms.org/go/media-centre:monthly-comment, he was chairing one of their committees until 2005. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Mitchell Jean A Date: Thursday, November 12, 2009 19:08 Subject: RE: [Histonet] FW: RIP Russ Allison To: "Morken, Tim" , histonet@lists.utsouthwestern.edu > I had the great privilege to speak with Russ Allison at a > conference in Australia several years ago. He was a funny > man, he was a wild man and enjoyable to be around. His > birthday was October 31st - and a Halloween has never gone by > since or will go by that I won't think of him. Cheers Russ!!!! > > Jean Mitchell > UWHC- Neuromuscular Laboratory > Madison, WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Morken, Tim > Sent: Thu 11/12/2009 5:36 PM > To: Hotmail; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] FW: RIP Russ Allison > > > > I'm sorry to hear that. I met Russ several times at NSH over the > years and had several email correspondences with him. He was > always helpful and a great guy. > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Hotmail > Sent: Thursday, November 12, 2009 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FW: RIP Russ Allison > > > > Vintage histonetters will remember the valuable contributions to > our body of > knowledge made by Russ Allison. I was saddened to hear of > his recent > passing after a seven year battle with Alzheimers. I'll > not presume to > eulogize him, there are many who knew him better and longer, > suffice it to > say you would have to go a long way to find a kinder, more > helpful man with > as great a passion for laboratory science, scientific education > and Welsh > rugby. On his annual trips to the US to present workshops > at NSH meetings > he would always find time for a pint or two and spirited > discussion about > the progress of the profession on both sides of the > pond. The following > is the URL of an obituary of Russ by the current chief executive > if the > Institute of Biomedical Scientists. > > > > http://www.ibms.org/go/media-centre:monthly-comment > > > > I'm off to the beer aisle so I can lift a Brains in his honour > tonight.Cheers, Russ and farewell. > > > > Simon Smith BSc MIBMS > > Chief Technical Officer > > Histion LLC > > 2615 W Casino Rd Unit 6G > > Everett, WA 98204 > > smiths@Histion.com > > T: (206) 953 1386 > > F: (425) 353-3604 > > > > LEGAL NOTICE > > Unless expressly stated otherwise, this message is confidential > and may be > privileged. It is intended for the addressee(s) only. Access to > this e-mail > by anyone else is unauthorised. If you are not an adressee, any > disclosureor copying of the contents of this e-mail or any > action taken (or not taken) > in reliance on it is unauthorised and may be unlawful. If you > are not an > addressee, please inform the sender immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joycefr <@t> frontiernet.net Sat Nov 14 10:31:48 2009 From: joycefr <@t> frontiernet.net (Joyce Friedland) Date: Sat Nov 14 10:31:46 2009 Subject: [Histonet] FW: RIP Russ Allison In-Reply-To: References: <1AAF670737F193429070841C6B2ADD4CF76A2DB8@EXMBMCB15.ucsfmedicalcenter.org> <2108AECB05DBFF48A9C436A792155740010C3794@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: <1704E335-6290-48C7-960C-4242E6B2A683@frontiernet.net> Having just lost my Mom to AD I can tell you that many people are able to conceal their disease for a long time. Joyce On Nov 14, 2009, at 12:15 AM, John Kiernan wrote: > I don't think Russ can have had a "battle with Alzheimer's for 7 > years". He was in good mental condition in Nov 2001, as Jean > Mitchell can also attest. (I was at the same conference.) According > to his IBMS obituary, http://www.ibms.org/go/media-centre:monthly-comment > , he was chairing one of their committees until 2005. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Mitchell Jean A > Date: Thursday, November 12, 2009 19:08 > Subject: RE: [Histonet] FW: RIP Russ Allison > To: "Morken, Tim" , histonet@lists.utsouthwestern.edu > >> I had the great privilege to speak with Russ Allison at a >> conference in Australia several years ago. He was a funny >> man, he was a wild man and enjoyable to be around. His >> birthday was October 31st - and a Halloween has never gone by >> since or will go by that I won't think of him. Cheers Russ!!!! >> >> Jean Mitchell >> UWHC- Neuromuscular Laboratory >> Madison, WI >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of >> Morken, Tim >> Sent: Thu 11/12/2009 5:36 PM >> To: Hotmail; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] FW: RIP Russ Allison >> >> >> >> I'm sorry to hear that. I met Russ several times at NSH over the >> years and had several email correspondences with him. He was >> always helpful and a great guy. >> >> Tim Morken >> Supervisor, Histology / IPOX >> UCSF Medical Center >> San Francisco, CA >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >> bounces@lists.utsouthwestern.edu] On Behalf Of Hotmail >> Sent: Thursday, November 12, 2009 2:39 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] FW: RIP Russ Allison >> >> >> >> Vintage histonetters will remember the valuable contributions to >> our body of >> knowledge made by Russ Allison. I was saddened to hear of >> his recent >> passing after a seven year battle with Alzheimers. I'll >> not presume to >> eulogize him, there are many who knew him better and longer, >> suffice it to >> say you would have to go a long way to find a kinder, more >> helpful man with >> as great a passion for laboratory science, scientific education >> and Welsh >> rugby. On his annual trips to the US to present workshops >> at NSH meetings >> he would always find time for a pint or two and spirited >> discussion about >> the progress of the profession on both sides of the >> pond. The following >> is the URL of an obituary of Russ by the current chief executive >> if the >> Institute of Biomedical Scientists. >> >> >> >> http://www.ibms.org/go/media-centre:monthly-comment >> >> >> >> I'm off to the beer aisle so I can lift a Brains in his honour >> tonight.Cheers, Russ and farewell. >> >> >> >> Simon Smith BSc MIBMS >> >> Chief Technical Officer >> >> Histion LLC >> >> 2615 W Casino Rd Unit 6G >> >> Everett, WA 98204 >> >> smiths@Histion.com >> >> T: (206) 953 1386 >> >> F: (425) 353-3604 >> >> >> >> LEGAL NOTICE >> >> Unless expressly stated otherwise, this message is confidential >> and may be >> privileged. It is intended for the addressee(s) only. Access to >> this e-mail >> by anyone else is unauthorised. If you are not an adressee, any >> disclosureor copying of the contents of this e-mail or any >> action taken (or not taken) >> in reliance on it is unauthorised and may be unlawful. If you >> are not an >> addressee, please inform the sender immediately. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Nov 14 12:10:32 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Nov 14 12:12:40 2009 Subject: [Histonet] Re: Ki67 Ab and specific antigen retrieval Message-ID: <11D9615B89C10747B1C985966A63D7CA2C3F25A2BA@KCL-MAIL04.kclad.ds.kcl.ac.uk> Have a look at this site, in their Image Gallery/protocols. http://www.immunoportal.com/modules.php?name=Forums Good luck. carl From pruegg <@t> ihctech.net Sat Nov 14 13:32:07 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Nov 14 13:32:49 2009 Subject: SPAM-LOW: [Histonet] Re: B5 alternate In-Reply-To: References: Message-ID: Good luck with that idea of sitting in formalin overnight, most of the Pathologist I have worked with over the last 20 years seem to be getting more resistant rather than more tolerant to that idea, to my chagrin. Cheers, Patsy Btw I was saddened to hear of Russ Allison's passing. I did consider him a good friend and knowledgeable histopathology colleague, one of the great ones left us in his passing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, November 13, 2009 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: B5 alternate I loved B5 fixative, and before that Zenker's, but they're gone and they're not coming back. In my personal expereience in a number of pathology services, the various muchly-touted B5 substitutes offer very little morphologic advantage over neutral buffered formalin. Unless it's fixed pretty early in the morning, tissue needs to sit overnight in neutral buffered formalin before being decalcified or processed. Pathologists and their clinicians are eventually going to have to learn to live with this delay. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Nov 14 13:39:33 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Nov 14 13:40:18 2009 Subject: SPAM-LOW: Re: RE: [Histonet] FW: RIP Russ Allison In-Reply-To: References: <1AAF670737F193429070841C6B2ADD4CF76A2DB8@EXMBMCB15.ucsfmedicalcenter.org><2108AECB05DBFF48A9C436A792155740010C3794@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: <3318C6E61A554C0D81BB990DA693042F@prueggihctechlt> Btw I was saddened to hear of Russ Allison's passing. I did consider him a good friend and knowledgeable histopathology colleague, one of the great ones left us in his passing. Regards, Patsy Ps I saw Russ in passing at NSH in 2002, and although he did not recognize me (I looked different that year since I had lost 50 lbs from a medical treatment) he sent me an email as soon as we got home apologizing for not recognizing me (I guess someone pointed that out to him) and seemed really sharp for having recognized that he had made that mistake, I had no idea that he was suffering from AD, I did miss seeing him at NSH the last few years. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Friday, November 13, 2009 10:15 PM To: Mitchell Jean A Cc: histonet@lists.utsouthwestern.edu; Morken,Tim Subject: SPAM-LOW: Re: RE: [Histonet] FW: RIP Russ Allison I don't think Russ can have had a "battle with Alzheimer's for 7 years". He was in good mental condition in Nov 2001, as Jean Mitchell can also attest. (I was at the same conference.) According to his IBMS obituary, http://www.ibms.org/go/media-centre:monthly-comment, he was chairing one of their committees until 2005. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Mitchell Jean A Date: Thursday, November 12, 2009 19:08 Subject: RE: [Histonet] FW: RIP Russ Allison To: "Morken, Tim" , histonet@lists.utsouthwestern.edu > I had the great privilege to speak with Russ Allison at a > conference in Australia several years ago. He was a funny > man, he was a wild man and enjoyable to be around. His > birthday was October 31st - and a Halloween has never gone by > since or will go by that I won't think of him. Cheers Russ!!!! > > Jean Mitchell > UWHC- Neuromuscular Laboratory > Madison, WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Morken, Tim > Sent: Thu 11/12/2009 5:36 PM > To: Hotmail; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] FW: RIP Russ Allison > > > > I'm sorry to hear that. I met Russ several times at NSH over the > years and had several email correspondences with him. He was > always helpful and a great guy. > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Hotmail > Sent: Thursday, November 12, 2009 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FW: RIP Russ Allison > > > > Vintage histonetters will remember the valuable contributions to > our body of > knowledge made by Russ Allison. I was saddened to hear of > his recent > passing after a seven year battle with Alzheimers. I'll > not presume to > eulogize him, there are many who knew him better and longer, > suffice it to > say you would have to go a long way to find a kinder, more > helpful man with > as great a passion for laboratory science, scientific education > and Welsh > rugby. On his annual trips to the US to present workshops > at NSH meetings > he would always find time for a pint or two and spirited > discussion about > the progress of the profession on both sides of the > pond. The following > is the URL of an obituary of Russ by the current chief executive > if the > Institute of Biomedical Scientists. > > > > http://www.ibms.org/go/media-centre:monthly-comment > > > > I'm off to the beer aisle so I can lift a Brains in his honour > tonight.Cheers, Russ and farewell. > > > > Simon Smith BSc MIBMS > > Chief Technical Officer > > Histion LLC > > 2615 W Casino Rd Unit 6G > > Everett, WA 98204 > > smiths@Histion.com > > T: (206) 953 1386 > > F: (425) 353-3604 > > > > LEGAL NOTICE > > Unless expressly stated otherwise, this message is confidential > and may be > privileged. It is intended for the addressee(s) only. Access to > this e-mail > by anyone else is unauthorised. If you are not an adressee, any > disclosureor copying of the contents of this e-mail or any > action taken (or not taken) > in reliance on it is unauthorised and may be unlawful. If you > are not an > addressee, please inform the sender immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Nov 14 22:55:19 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Nov 14 22:55:23 2009 Subject: [Histonet] Re: amnion tissue Message-ID: Patsy Ruegg asks about preparation of 3 x 2 inch pieces of human "amnion tissue". Assuming that fetal membranes from a term placenta are meant, the standard way to embed these is by rolling them. There are several ways to do this. I prefer to cut a strip of the membranes, roll them around a wooden applicator stick, drive a common straight pin through the membranes perpendicular (skew) to the stick, pull the stick out, and cut one or more 5 mm sections of the roll next to the pin. This roll is placed in a cassette, and subsequently embedded. On a different topic, Merc? Mart? Gaudes at the University of Barcelona has achieved a first for Histonet - the first post in Catalan that I can remember. >>Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. "Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business."<< Catalan is a language related to Spanish, spoken in Catalonia in Spain. Bob Richmond Samurai Pathologist Knoxville TN From relia1 <@t> earthlink.net Mon Nov 16 08:56:57 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Nov 16 08:57:01 2009 Subject: [Histonet] RELIA Solutions Histology Job Alert Message-ID: Hi Histonetters!! I hope everyone is having a great Monday. I have a few new jobs I want to tell you about. These are full time permanent M-F dayshift positions. All of these clients offer an excellent salary, benefits and relocation assistance. Here is a list of my newest positions: Jackson, MI - Histotech - Dermpath Lab Pensacola, FL - Private Pathology Lab Charlotte, NC - Pathologist's Assistant new grads welcome! Here is a list of the other great opportunities I am currently working on. These are fulltime permanent M-F dayshift positions and these clients also offer a great salary, benefits and relocation assistance. Fresno, CA - Pathology Manager Charlotte, NC - Histology Manager Spokane, WA - Histology Manager Orange/Rockland County, NY - Histotech *All of these positions are open now and can start you immediately and are also willing to wait until after the holidays if that is your preference.* If you or anyone you know might be interested in hearing more about any of these opportunities please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. I am available to talk before during and after hours at your convenience. Thanks for taking time out of your busy day to read this -Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From katherine-walters <@t> uiowa.edu Mon Nov 16 09:09:10 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Nov 16 09:09:20 2009 Subject: [Histonet] Immunohistochemistry fixation Message-ID: Has anyone has any experience using immunohistochemistry with the following fixation? Polyethylene glycol (PEG) solution: 25% glycol, 10% ethanol (95%), 10% formaldehyde (37%), and 55% distilled water. Specifically if there are any enzymatic reactions that I should avoid/block for/etc. Thanks for any hints-I am pretty much wed to this fixative. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf From relia1 <@t> earthlink.net Mon Nov 16 09:49:22 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Nov 16 09:49:23 2009 Subject: [Histonet] Question on certification requirements for grossing in the state of Michigan Message-ID: Hi Histonetters, I have a quick question and am hoping someone can either answer it or send me to the right place to get the answer. Is an ASCP HTL certification required in Michigan in order to be able to gross or is an HT allowed to gross as well? Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From SSeguin <@t> hrsrh.on.ca Mon Nov 16 10:49:45 2009 From: SSeguin <@t> hrsrh.on.ca (Seguin, Suzanne) Date: Mon Nov 16 10:49:51 2009 Subject: [Histonet] Re:FS stainer Message-ID: Hello, Does anyone know of any FS stainer other then the Shandon Linistat Linear Stainer? Thanks Sue Sudbury Regional Hospital From achertcoff <@t> anlis.gov.ar Mon Nov 16 12:02:23 2009 From: achertcoff <@t> anlis.gov.ar (Agustin Victor Chertcoff) Date: Mon Nov 16 12:02:39 2009 Subject: [Histonet] Knifemaker LKB supply Message-ID: <23afd74d0911161002o2c8fbd5xb70ae6f0a6067ec6@mail.gmail.com> I need help. It is urgent to replace in my lab off electron microscopy, the cuting wheel for model 7801b lkb knifemaker. I have not found it here, and I'm looking in other hemisphere or country. Thanks all! Ht Agustin V Chertcoff Electron Microscopy Service National Institute off Microbiology C G Malbran Buenos Aires Argentina From rsrichmond <@t> gmail.com Mon Nov 16 13:01:54 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Nov 16 13:02:00 2009 Subject: [Histonet] Re: Immunohistochemistry fixation Message-ID: Katherine Walters, Histology Director, Central Microscopy Research Facilities, University of Iowa asks: >>Has anyone has any experience using immunohistochemistry with the following fixation? Polyethylene glycol (PEG) solution: 25% glycol, 10% ethanol (95%), 10% formaldehyde (37%), and 55% distilled water. Specifically if there are any enzymatic reactions that I should avoid/block for/etc. - Thanks for any hints-I am pretty much wed to this fixative. You may be wed to it, but you may be being two-timed if you are. Polyethylene glycol (trade name Carbowax, from Dow Chemical) is not a single substance, but comes in a wide range of molecular weights with physical state anywhere from a watery liquid to a paraffin-like solid. See the Wikipedia article. If you're using a commercial product containing PEG, the manufacturer may know the answer. If you're compounding it yourself, you may just have to try the IHC's and see. Any publications should cite the molecular weight (or Carbowax number) of the PEG in the fixative. Bob Richmond Samurai Pathologist Knoxville TN From TMcNemar <@t> lmhealth.org Mon Nov 16 13:16:48 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Nov 16 13:17:24 2009 Subject: [Histonet] Cryostat decontamination... Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E152@lmhsmail.lmhealth.org> Hello all, We have always used absolute alcohol to decontaminate our cryostat and wondered what others use. This has been brought up by an article in the September issue of CAP Today that talks about biosafety when doing frozens (specifically talks about TB). As a related question, how many institutions require the wearing on an N95 respirator when doing frozens? The article states that diluted alcohol is more effective "because the presence of water causes proteins to denature more quickly." I just did the decontamination of our cryostat last weekend and used 70% alcohol as the article suggested. The alcohol evaporated and I was left with beads/drops of water that I had to then dry by hand. I guess the alternative would be is just to go over it again with aboslute after usiing 70%. The article goes on to recommend that personnel should wear N95 masks that are fit tested on a yearly basis. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From jclark <@t> pcnm.com Mon Nov 16 13:27:14 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Nov 16 13:27:20 2009 Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 17 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01142CF8@mail.pcnm.com> Sue, TBS had a frozen section stainer on display at NSH this year. I don't have the information right here in front of me, but if you are interested I will forward it to you. Joanne Clark, HT(ASCP)MLT(CSMLS) Path Consultants of New Mexico Roswell, NM Message: 4 Date: Mon, 16 Nov 2009 11:49:45 -0500 From: "Seguin, Suzanne" Subject: [Histonet] Re:FS stainer To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Does anyone know of any FS stainer other then the Shandon Linistat Linear Stainer? Thanks Sue Sudbury Regional Hospital ------------------------------ From liz <@t> premierlab.com Mon Nov 16 13:34:33 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Nov 16 13:34:42 2009 Subject: [Histonet] Cryostat decontamination... In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E152@lmhsmail.lmhealth.org> Message-ID: >From what I understand if you require that an individual wear a N95 respirator when they are sectioning frozen sections then you are also required to have that respirator fit tested yearly. We are a research lab that does quite a bit of work on TB samples. We do not section TB infected samples on a cryostat. All samples that we receive have been fixed in 10% NBF for several days and then transferred to 70% alcohol. When I was in a clinical lab, we would not section frozen sections of lung samples if they wanted to rule out TB, the pathologist would recommend to process to paraffin first. In fact since the samples are generated in a biosafety level 2 or 3 facility, they can not leave that facility unless the samples have been fixed and rendered non infectious. Frozen sections would need to be prepared within that biosafety facility with all of the appropriate PPE's in place. The University that we work with has tested these samples via culture after their fixation and alcohol procedure. We do however offer the N95 respirator to the techs and they can wear it if they want to, when they are grossing, embedding or sectioning these samples. But since it is voluntary we do not have to fit test, we have a specific procedure that covers this. This protocol has been developed with the help of our local OSHA rep. The other thing is we use a special vacumme with a hepa filter to vacume up the paraffin trimmings. OSHA has a program for small businesses and will work with them to make sure that they are within compliance, they have been very helpful to us here. Just go to the OSHA website. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, November 16, 2009 12:17 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Cryostat decontamination... Hello all, We have always used absolute alcohol to decontaminate our cryostat and wondered what others use. This has been brought up by an article in the September issue of CAP Today that talks about biosafety when doing frozens (specifically talks about TB). As a related question, how many institutions require the wearing on an N95 respirator when doing frozens? The article states that diluted alcohol is more effective "because the presence of water causes proteins to denature more quickly." I just did the decontamination of our cryostat last weekend and used 70% alcohol as the article suggested. The alcohol evaporated and I was left with beads/drops of water that I had to then dry by hand. I guess the alternative would be is just to go over it again with aboslute after usiing 70%. The article goes on to recommend that personnel should wear N95 masks that are fit tested on a yearly basis. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From o.isaac24 <@t> yahoo.com Mon Nov 16 14:22:40 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Mon Nov 16 14:22:44 2009 Subject: [Histonet] NEW POSITION WANTED PLS Message-ID: <460802.66373.qm@web111603.mail.gq1.yahoo.com> ? Hi, ?? I am looking for a new HISTOTECH/IHC position. I am? both HT(ASCP) and HTL(ASCP) certified. I am open to relocation most?especially , the MIDWEST, NORTH EAST, SOUTH EAST and the NORTH WEST. ?? I also have some management experience. ?Isaac. From ncollins <@t> system1.net Mon Nov 16 14:41:58 2009 From: ncollins <@t> system1.net (Noelle Collins) Date: Mon Nov 16 14:37:46 2009 Subject: [Histonet] Pathology Opportunities Message-ID: Good afternoon! I am working on some great opportunities for Pathologists, including an opportunity with a specialty diagnostics group looking for a Pathologist with a Surgical Oncologic background. If anyone knows of any Pathologists looking for opportunities I would greatly appreciate it if you would pass along my contact information to them. Thanks for any help you can provide! Noelle Collins Executive Recruiter System 1 Search 864-528-5065 ncollins@system1.net http://www.system1.net/Pathology.htm Please join me at LinkedIn: http://www.linkedin.com/in/noellehcollins From lost.dragonfly <@t> yahoo.com Mon Nov 16 15:44:48 2009 From: lost.dragonfly <@t> yahoo.com (Una McGiven) Date: Mon Nov 16 15:44:53 2009 Subject: [Histonet] CAP Message-ID: <621519.35493.qm@web110315.mail.gq1.yahoo.com> Hi All, ? My lab would like to switch from JCAHO to CAP.? What are the differences?? We are mostly interested in: Are two patient identifiers necessary on specimen containers (like under JCAHO)? What are the education requirements for grossing of specimens that require inking and representative sections/margin check/etc? QA/QC? ? I know that the checklist is available but it is $500 and we don't want to spend that unless we know that this is a good idea... ? Thanks! Una in Colorado? From jkiernan <@t> uwo.ca Mon Nov 16 15:53:24 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Nov 16 15:53:29 2009 Subject: [Histonet] B5 alternate Message-ID: What is this "B plus" fixative? Is it a trade-secret brew? John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Anne van Binsbergen Date: Friday, November 13, 2009 10:01 Subject: Re: [Histonet] B5 alternate To: Drew Meyer <41dmb41@gmail.com> Cc: "Knutson, Deanne" , "histonet@lists.utsouthwestern.edu" > B plus is the one - we use it as stated and it works like a charm > immunos are good too - we are proudly mercury free > Annie > > 2009/11/13 Drew Meyer <41dmb41@gmail.com> > > > In my lab, we're using B-Plus Fixative by BBC > Biochemical. I know > > many other labs that are also using it as their B5 > alternative. The > > biggest pro, of course, is that it's mercury free and not hazardous. > > Also, it's not as sensitive to over-fixation like B5 is. The > > guideline I use is 4 hours minimum fixation for Bone Marrows and > > Lymphoid tissue. After that, routine formalin processing > is all you > > need to do. You can, however, leave the specimen in B- > Plus for up to > > 48 hours without adversely effecting the tissue sample. > > > > Hope this help. > > > > Drew Meyer > > > > On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne > > wrote: > > > Am looking for advice on what alternate fixative to use to > replace B5. > > What > > > do the majority of you histonetters use? Does it work > well with immunos? > > > The pros and cons? Thank you for your recommendations. > > > > > > > > > > > > Deanne Knutson > > > > > > Anatomic Pathology Supervisor > > > > > > St. Alexius Medical Center > > > > > > 900 E. Broadway > > > > > > Bismarck, North Dakota 58506 > > > > > > (701)-530-6730 > > > > > > dknutson@primecare.org > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrickintl <@t> sbcglobal.net Mon Nov 16 16:12:48 2009 From: patrickintl <@t> sbcglobal.net (Thomas Patrick Chuna) Date: Mon Nov 16 16:12:52 2009 Subject: [Histonet] Dermatopathologist Opportunity Message-ID: <660858.43080.qm@web82507.mail.mud.yahoo.com> Hello Histonet! ? I'm Tom, and I am a recruiter. ? The?opportunity I'm bringing you today is for practicing Dermpaths, but if you are close to finishing your Dermpath fellowship, our client would love to meet you as well. ? The location is Dallas Texas, and?very generous relocation assistance? is available. Regardless of where you live in the US, this opportunity might be right for you. ? Our client is a known, established?clinical diagnostics organization and they are serious about growing their dermpath practice. Responses with a resume get a full position description and additional information.? ? Reply to: ?patrickintl@sbcglobal.net ? Thanks for the forum to share my opportunities with all of you. ? Best Regards, Tom ? ? Thomas Patrick Chuna - Owner Patrick International Specialist In Scientific Information Management Opportunities Email: patrickintl@sbcglobal.net Website: http://www.patrickinternational.net LinkedIn: http://www.linkedin.com/in/patrickinternational From 41dmb41 <@t> gmail.com Mon Nov 16 16:16:37 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Nov 16 16:16:51 2009 Subject: [Histonet] B5 alternate In-Reply-To: References: Message-ID: Essentially it's a zinc formalin. Drew Sent from my iPhone On Nov 16, 2009, at 4:53 PM, John Kiernan wrote: > What is this "B plus" fixative? Is it a trade-secret brew? > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Anne van Binsbergen > Date: Friday, November 13, 2009 10:01 > Subject: Re: [Histonet] B5 alternate > To: Drew Meyer <41dmb41@gmail.com> > Cc: "Knutson, Deanne" , "histonet@lists.utsouthwestern.edu > " > >> B plus is the one - we use it as stated and it works like a charm >> immunos are good too - we are proudly mercury free >> Annie >> >> 2009/11/13 Drew Meyer <41dmb41@gmail.com> >> >>> In my lab, we're using B-Plus Fixative by BBC >> Biochemical. I know >>> many other labs that are also using it as their B5 >> alternative. The >>> biggest pro, of course, is that it's mercury free and not hazardous. >>> Also, it's not as sensitive to over-fixation like B5 is. The >>> guideline I use is 4 hours minimum fixation for Bone Marrows and >>> Lymphoid tissue. After that, routine formalin processing >> is all you >>> need to do. You can, however, leave the specimen in B- >> Plus for up to >>> 48 hours without adversely effecting the tissue sample. >>> >>> Hope this help. >>> >>> Drew Meyer >>> >>> On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne >> > wrote: >>>> Am looking for advice on what alternate fixative to use to >> replace B5. >>> What >>>> do the majority of you histonetters use? Does it work >> well with immunos? >>>> The pros and cons? Thank you for your recommendations. >>>> >>>> >>>> >>>> Deanne Knutson >>>> >>>> Anatomic Pathology Supervisor >>>> >>>> St. Alexius Medical Center >>>> >>>> 900 E. Broadway >>>> >>>> Bismarck, North Dakota 58506 >>>> >>>> (701)-530-6730 >>>> >>>> dknutson@primecare.org >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> Anne van Binsbergen (Hope) >> Abu Dhabi >> UAE >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsantiago <@t> bellsouth.net Mon Nov 16 19:31:44 2009 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Mon Nov 16 19:31:47 2009 Subject: [Histonet] test Message-ID: <61977.22495.qm@web180414.mail.gq1.yahoo.com> This is a test. From tifei <@t> foxmail.com Mon Nov 16 21:52:10 2009 From: tifei <@t> foxmail.com (TF) Date: Mon Nov 16 21:52:20 2009 Subject: [Histonet] BrdU & EM Message-ID: <200911171152096246182@foxmail.com> Just wondering anyone has an idea to solve this problem? __________________________________________________________ Yes, I have tried with all kinds of concentrations of HCl and different times of incubation as well. The BrdU labelling works even with 10 minutes of HCl incubation (this is not very consistent, though) but the ultrastructure is 'dead' by that time. Has anyone got any experience with breaking DNA strands using UV light? What's the wavelength that could be used and how long does the tissue need to be exposed? My first try was our fluorescent microscope (at 360-370 nm), exposure time 1 hour, but it doesn't seem to do the job . Szilvi Mezey Subject: RE: [Histonet] BrdU and EM? Date sent: Thu, 18 Sep 2003 09:40:23 +0100 From: "Edwards, R.E." To: "Mezey Szilvia" > Have you tried cutting down the HCl time to a minimum????? > Richard Edwards > MRC TOX UNIT....U.K....... > > > > -----Original Message----- > From: Mezey Szilvia [mailto:mezey@ana.sote.hu] > Sent: 17 September 2003 12:54 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] BrdU and EM? > > > Hello All, > > I'm trying to do BrdU staining (in paraformaldehyde fixed, free- > floating sections) and preserve the ultrastructure of the tissue for > EM at the same time. HCl works fine for denaturing DNA for BrdU- > ICC but doesn't leave much of the tissue for EM. DNase would be > more EM-friendly but doesn't penetrate the tissue enough. > > Does anybody know a method that could provide a fair enough > compromise between BrdU and EM? Maybe by increasing the > penetration of DNase? > > Best regards to you all, > > Szilvi > > Szilvia Mezey > PhD student > Semmelweis University > Dept. of Anatomy, Histology and Embryology > Tuzolto u. 58. Budapest, 1094, Hungary > T.: +36-12156920/3687 > F.: +36-12155158 > E-mail: mezey@ana.sote.hu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Szilvia Mezey PhD student Semmelweis University Dept. of Anatomy, Histology and Embryology Tuzolto u. 58. Budapest, 1094, Hungary T.: +36-12156920/3687 F.: +36-12155158 E-mail: mezey@ana.sote.hu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009-11-17 TF From brett_connolly <@t> merck.com Tue Nov 17 08:48:00 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Nov 17 08:48:17 2009 Subject: [Histonet] Thymidine Kinase 1 antibody for rat tissue? Message-ID: <63EA0607835FBA4689CEA9EA8B482692028295EF@usctmx1141.merck.com> Hi all, Looking for an anti-TK1 antibody for IHC on rat tissue....frozen or preferably FFPE. If you know of one, please share. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From MMorin <@t> Lifespan.org Tue Nov 17 09:12:04 2009 From: MMorin <@t> Lifespan.org (Morin, Mary Anne) Date: Tue Nov 17 09:12:09 2009 Subject: [Histonet] Control for Spirochete Message-ID: <130E8991F210424096EFC6F42EA33B2463956C@LSCOEXCH1.lsmaster.lifespan.org> Hi, I was wondering if anyone knows where I can obtain good control material for spirochete. We recently bought auto stainers from Ventana and all the tissue must be on Plus slides only. We currently have slides from Newcomer Supply, but they are coated with something, and the Steiner stain didn't work. I'm interested in either paraffin blocks or a company that uses Plus slides. Thanks for your help. Marie Anne Morin HT. ASCP Path Tech Specialist Histology Lab Rhode Island Hospital Office 444-7196 Pager 350-7384 Fax 444-8514 From lab.mef <@t> smmc.org Tue Nov 17 09:16:09 2009 From: lab.mef <@t> smmc.org (Meredith Fuller-Fedorczyk) Date: Tue Nov 17 09:16:21 2009 Subject: [Histonet] Control for Spirochete References: <130E8991F210424096EFC6F42EA33B2463956C@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <44BF7C50FB1B410E9AB079B9BB5A322D@PCLAB11> Mercedes Medical sells controls ----- Original Message ----- From: "Morin, Mary Anne" To: Sent: Tuesday, November 17, 2009 10:12 AM Subject: [Histonet] Control for Spirochete Hi, I was wondering if anyone knows where I can obtain good control material for spirochete. We recently bought auto stainers from Ventana and all the tissue must be on Plus slides only. We currently have slides from Newcomer Supply, but they are coated with something, and the Steiner stain didn't work. I'm interested in either paraffin blocks or a company that uses Plus slides. Thanks for your help. Marie Anne Morin HT. ASCP Path Tech Specialist Histology Lab Rhode Island Hospital Office 444-7196 Pager 350-7384 Fax 444-8514 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Nov 17 10:57:51 2009 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Tue Nov 17 10:57:57 2009 Subject: [Histonet] slide label printer ribbon type? Message-ID: Hi everyone. We are finally able to print slide labels with our new computer system! Wondering what type of printer ribbon should be used with our Zebra slide labellers? Wax wax/resin or resin? Any suggestions? (Obviously xylene/alcohol, etc resistant is a must). Thank you. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From shive003 <@t> umn.edu Tue Nov 17 10:58:02 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Nov 17 10:58:14 2009 Subject: [Histonet] EMCV antibody Message-ID: Does anyone know of a commercial source for anti-EMCV (porcine encephalomyocarditis virus)? If so, please contact me personally at my email address below. Thanks in advance, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) From AGarrison <@t> martek.com Tue Nov 17 11:02:17 2009 From: AGarrison <@t> martek.com (Augusta Garrison) Date: Tue Nov 17 11:01:37 2009 Subject: [Histonet] The best histology molds Message-ID: Hi all, Does anyone have any link, product or ordering info for histology molds I can purchase to paraffin embed rat brains . More specifically I need the size of histology molds that are appropriate for rat brains that are age 0-adult. Thanks so much!!!! Augusta E. Garrison Senior Research Associate Discovery-Neuroscience Martek Biosciences Corporation 4909 Nautilus Court North, Suite 208 Boulder, CO 80301 Tel 303-357-2812 From victor <@t> pathology.washington.edu Tue Nov 17 11:23:26 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Nov 17 11:27:23 2009 Subject: [Histonet] slide label printer ribbon type? In-Reply-To: References: Message-ID: <4B02DC0E.6010103@pathology.washington.edu> Jacqueline, It is important to match the ribbon to the label stock. For our polyester slide label (chemical resistant) we use a premium resin ribbon. We use a paper label for specimen and requisitions that uses a wax ribbon. Your label vendor should be able to recommend the proper ribbon. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jacqueline Farnsworth wrote: > Hi everyone. > We are finally able to print slide labels with our new computer system! Wondering what type of printer ribbon should be used with our Zebra slide labellers? Wax wax/resin or resin? Any suggestions? (Obviously xylene/alcohol, etc resistant is a must). > > Thank you. > > Jacqueline Farnsworth > Anatomic Pathology, Tech III > Foothills Medical Centre > Calgary Laboratory Services > P Please consider the environment before printing this email. > > ________________________________ > This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kimberly.Poole <@t> drdc-rddc.gc.ca Tue Nov 17 12:46:18 2009 From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly) Date: Tue Nov 17 12:46:26 2009 Subject: [Histonet] Acid/Alcohol Message-ID: <42DFE1A029181B4B8CCBA7261B52D765010EF9B5@suffieldex01.suffield.drdc-rddc.gc.ca> Good Morning, I have a question regarding some histology Methods. I am about to order acid/alcohol and I am not sure why I am using it and what percentage I should get (1%, 5%, 0.5%). I also have a question regarding the staining procedure itself. Our staining procedure has about 27 steps in it and I am wondering if I need to have 27 glass containers for the steps? Thanks for your help! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From liz <@t> premierlab.com Tue Nov 17 12:55:33 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Nov 17 12:55:38 2009 Subject: [Histonet] Acid/Alcohol In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D765010EF9B5@suffieldex01.suffield.drdc-rddc.gc.ca> Message-ID: Kimberly I would make my acid solution up from scratch rather than purchasing from a vendor, that way you will have the flexibility to make up whatever concentration is needed. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poole, Kimberly Sent: Tuesday, November 17, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acid/Alcohol Good Morning, I have a question regarding some histology Methods. I am about to order acid/alcohol and I am not sure why I am using it and what percentage I should get (1%, 5%, 0.5%). I also have a question regarding the staining procedure itself. Our staining procedure has about 27 steps in it and I am wondering if I need to have 27 glass containers for the steps? Thanks for your help! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Tue Nov 17 13:43:57 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Nov 17 13:44:09 2009 Subject: [Histonet] re: New CAP question Message-ID: Does anyone have any insight on this question as to what they are expecting to see? Personally email me if you have any insight. **NEW** 06/15/2009 GEN.40104 Phase II N/A YES NO Are instructions distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens? NOTE: Instructions should be documented for all applicable tissue and cytologic specimens, including biopsies, resections, Pap tests, sputum, washings, brushings, body fluids, fine needle aspirations, etc. These instructions must be included in the procedure or user manuals at all sites where specimens are collected (e.g., nursing stations, clinics, physicians' offices). Instructions must include proper fixation of slides and tissue specimens. Thanks in advance! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From rjbuesa <@t> yahoo.com Tue Nov 17 14:34:27 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 17 14:34:32 2009 Subject: [Histonet] Acid alcohol: Message-ID: <211411.88137.qm@web65716.mail.ac4.yahoo.com> It seems from your question that you stain manually. In that case I would suggest you that you should prepare your own acid alcohol and I would also suggest it to be 0.5% because a mild acidity will allow you to be more precise in the differentiation step.If you have 27 steps (which I think are too many) you will need one container per step. Ren? J. From rjbuesa <@t> yahoo.com Tue Nov 17 14:38:24 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 17 14:38:28 2009 Subject: [Histonet] re: New CAP question In-Reply-To: Message-ID: <459972.37118.qm@web65702.mail.ac4.yahoo.com> First excuse me if I don't e-mail you personally because I am a firm believer that the answers should be for all to share. ? The question is explained in the "Note" and this means that you have to give instructions to all those providing you samples as to how to collect them, send them to you, etc. This should be part of your Cytology SOP. Ren? J. --- On Tue, 11/17/09, Renko, Heather D. wrote: From: Renko, Heather D. Subject: [Histonet] re: New CAP question To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 17, 2009, 2:43 PM Does anyone have any insight on this question as to what they are expecting to see?? Personally email me if you have any insight. **NEW**? ? ???06/15/2009 GEN.40104 Phase II N/A? YES? NO Are instructions distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens? NOTE: Instructions should be documented for all applicable tissue and cytologic specimens, including biopsies, resections, Pap tests, sputum, washings, brushings, body fluids, fine needle aspirations, etc. These instructions must be included in the procedure or user manuals at all sites where specimens are collected (e.g., nursing stations, clinics, physicians' offices).? Instructions must include proper fixation of slides and tissue specimens. Thanks in advance! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.Tyler <@t> uct.ac.za Wed Nov 18 01:47:49 2009 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Wed Nov 18 01:48:01 2009 Subject: [Histonet] Fwd: foxp3 ab ihc Message-ID: <4B03C2C50200009000080256@gwiasmtp.uct.ac.za> Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather From tay.kian <@t> gmail.com Wed Nov 18 03:54:08 2009 From: tay.kian <@t> gmail.com (tay kianlee) Date: Wed Nov 18 03:54:12 2009 Subject: [Histonet] ATP 9.4 AND ATP 4.3 Message-ID: <3f12a0be0911180154h16c2402agbd9a0bc0bdb13fc2@mail.gmail.com> Does anybody know tips how to do the muscle biopsy for the above tests? Especially the preparation for the reagents needed. From richarddrahci <@t> gmail.com Wed Nov 18 04:19:29 2009 From: richarddrahci <@t> gmail.com (Richard .) Date: Wed Nov 18 04:19:35 2009 Subject: [Histonet] Re: Fwd: foxp3 ab ihc Message-ID: <88613e830911180219i2cd5e042u95d9bfbac436c996@mail.gmail.com> http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=FoxP3 Marilyn Tyler Tue, 17 Nov 2009 23:55:18 -0800 Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Wed Nov 18 06:35:11 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Nov 18 06:35:16 2009 Subject: [Histonet] IHC in Ontario Canada Message-ID: Hello, I've recently started working back in Canada and I am looking to find other techs that are familiar with the OLA regulations for IHCs. Seems to be a little different than the CAP guidelines. Sheila Adey HT MLT _________________________________________________________________ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819 From ian.montgomery <@t> bio.gla.ac.uk Wed Nov 18 06:48:29 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Nov 18 06:50:19 2009 Subject: FW: [Histonet] ATP 9.4 AND ATP 4.3 Message-ID: <2A830667FD1A4850BE78FA4CC6809371@IBLS.GLA.AC.UK> Tay, You'll also need pH4.6 for the acid reversal and fibre typing. In my hands, the technique published by J.M. Round, et al, 1980. Histochem. J. 12 707-710 is the most consistent. All the solutions should be freshly prepared and pH adjusted using a newly calibrated pH meter. The sections should be cut just before staining. Before and after the cobalt make sure the sections are well rinsed or you'll get 'dirty' staining from the sulphide. I also change the bottle of sulphide regularly so buy the smallest quantity possible and change frequently. Sticking to these rules I get superb fibre typing. I've tried numerous techniques for ATPase but always return to the Joan Round technique. Any problems let me know and I'll e-mail the technique. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tay kianlee Sent: 18 November 2009 09:54 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ATP 9.4 AND ATP 4.3 Does anybody know tips how to do the muscle biopsy for the above tests? Especially the preparation for the reagents needed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Nov 18 07:13:02 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Nov 18 07:13:06 2009 Subject: [Histonet] CD68 on FFPE human tissue Message-ID: <713175.13991.qm@web50309.mail.re2.yahoo.com> What antibodies are people using for this marker? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From rgrow <@t> bmnet.com Wed Nov 18 07:52:57 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Wed Nov 18 07:53:00 2009 Subject: [Histonet] Re: The best histology molds In-Reply-To: Message-ID: Have you tried using an eye mold? Surgipath and Sakura sell super cassettes and molds. One suggestion: after processing eye, brain, etc. at embedding, top mold with a routine cassette that you transferred ID on. If you top the super mold with a super cassette, it is not very stable on the microtome. rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 19 Date: Tue, 17 Nov 2009 12:02:17 -0500 From: Augusta Garrison Subject: [Histonet] The best histology molds To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Does anyone have any link, product or ordering info for histology molds I can purchase to paraffin embed rat brains . More specifically I need the size of histology molds that are appropriate for rat brains that are age 0-adult. Thanks so much!!!! Augusta E. Garrison Senior Research Associate Discovery-Neuroscience Martek Biosciences Corporation 4909 Nautilus Court North, Suite 208 Boulder, CO 80301 Tel 303-357-2812Renee Grow, BA., HT (ASCP) From richarddrahci <@t> gmail.com Wed Nov 18 08:09:05 2009 From: richarddrahci <@t> gmail.com (Richard .) Date: Wed Nov 18 08:09:10 2009 Subject: [Histonet] CD68 on FFPE human tissue In-Reply-To: <713175.13991.qm@web50309.mail.re2.yahoo.com> References: <713175.13991.qm@web50309.mail.re2.yahoo.com> Message-ID: <88613e830911180609o592d1f39maac3661459b7f1df@mail.gmail.com> PG-M1 from dako 2009/11/18 Kim Merriam > What antibodies are people using for this marker? > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From W.E.J.Hoekert <@t> olvg.nl Wed Nov 18 08:34:42 2009 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Wed Nov 18 08:35:51 2009 Subject: [Histonet] CD68 on FFPE human tissue References: <713175.13991.qm@web50309.mail.re2.yahoo.com> <88613e830911180609o592d1f39maac3661459b7f1df@mail.gmail.com> Message-ID: <1190CB05C44B13409483514729C2FC360C0A4D@PAIT42.olvg.nl> Yes, we too. Pretreatment with trypsin. ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Richard . Verzonden: wo 18-11-2009 15:09 Aan: Kim Merriam CC: Histonet Onderwerp: Re: [Histonet] CD68 on FFPE human tissue PG-M1 from dako 2009/11/18 Kim Merriam > What antibodies are people using for this marker? > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From mpence <@t> grhs.net Wed Nov 18 09:12:52 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Nov 18 09:12:57 2009 Subject: [Histonet] re: New CAP question In-Reply-To: <459972.37118.qm@web65702.mail.ac4.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3C9B@is-e2k3.grhs.net> And it should also include what is acceptable for a specimen and what will be rejected. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 17, 2009 2:38 PM To: histonet@lists.utsouthwestern.edu; Heather D.Renko Subject: Re: [Histonet] re: New CAP question First excuse me if I don't e-mail you personally because I am a firm believer that the answers should be for all to share. ? The question is explained in the "Note" and this means that you have to give instructions to all those providing you samples as to how to collect them, send them to you, etc. This should be part of your Cytology SOP. Ren? J. --- On Tue, 11/17/09, Renko, Heather D. wrote: From: Renko, Heather D. Subject: [Histonet] re: New CAP question To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 17, 2009, 2:43 PM Does anyone have any insight on this question as to what they are expecting to see?? Personally email me if you have any insight. **NEW**? ? ???06/15/2009 GEN.40104 Phase II N/A? YES? NO Are instructions distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens? NOTE: Instructions should be documented for all applicable tissue and cytologic specimens, including biopsies, resections, Pap tests, sputum, washings, brushings, body fluids, fine needle aspirations, etc. These instructions must be included in the procedure or user manuals at all sites where specimens are collected (e.g., nursing stations, clinics, physicians' offices).? Instructions must include proper fixation of slides and tissue specimens. Thanks in advance! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From M.Walker <@t> hrsu.mrc.ac.uk Wed Nov 18 10:22:23 2009 From: M.Walker <@t> hrsu.mrc.ac.uk (Marion Walker) Date: Wed Nov 18 10:22:53 2009 Subject: [Histonet] In search of a reliable androgen receptor Message-ID: <86F334797DC6524A99AD9DD8F23A8B5002938BC9@mailserv.hrsu.mrc.ac.uk> We are trying to find a reliable AR antibody that works on human, primate, rat and mouse, bouins fixed, paraffin embedded tissue. Over the years we have used various antibodies but just recently we have had major problems with inconsistency and between batch variations. For many years we used a Santa Cruz antibody cat. No. sc-816 but when we bought a new vial it no longer worked. We tried several different vials from the company without success. We then tried an antibody from MyBiosource MBS3000466, also supplied by Abcam ab74272 which worked well when we got it at 1:1000 but after a very short time began to deteriorate and now works only at 1:100 and still falling. Most recently we tried an antibody from Genetex via Autogen Bioclear GTX73078. We initially bought one vial which seemed to work well and have just purchased five vials which we were assured were from the same batch and they did have the same batch numbers but on testing they gave different results. All antibodies were stored according to manufacturers instructions. So if anybody knows of a reliable AR antibody we would be very, very, very pleased to hear from you. Thanks in advance Marion Walker Human Reproductive Sciences Unit Edinburgh Scotland UK From wa7buxx <@t> yahoo.com Wed Nov 18 10:36:23 2009 From: wa7buxx <@t> yahoo.com (richard wells) Date: Wed Nov 18 10:36:27 2009 Subject: [Histonet] OSHA shoe requirements for histology Message-ID: <714075.28027.qm@web36305.mail.mud.yahoo.com> It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors.? Does anyone have any more information regarding this? From plucas <@t> biopath.org Wed Nov 18 12:06:41 2009 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Nov 18 12:06:51 2009 Subject: [Histonet] Industrial Hygienist/Fumes in lab Message-ID: <001301ca6879$e167d3d0$0f01a8c0@biopath.local> Hello everyone, I'm hoping someone can help me. We have a small room in the lab where the coverslipper and stainer are. We have the coverslipper attached to a fume hood which is attached to the ceiling to exhaust the fumes out of the building. The stainer has a charcoal filter inside the unit. We have an air purifier to help with ventilation and we have a fume hood to work under as well. With all these precautions, this area still gets a good amount of fumes lurking around for people to feel uncomfortable with. We do the badge monitoring and are always under the limits, but I still would like to get it better. What kind of ventilation solutions have you done to help minimize the fumes and/or do you have a contact of a Certified Industrial Hygienist? I would like one to come and take measurements or monitor the fumes in this area. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group From galinadeyneko <@t> yahoo.com Wed Nov 18 12:13:32 2009 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Wed Nov 18 12:13:35 2009 Subject: [Histonet] Re: Freezing of the sceletal muscles Message-ID: <219946.53957.qm@web33103.mail.mud.yahoo.com> Dear Colleagues. I have couple questions regarding freezing of the mouse / rat muscles. 1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful. 2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining, ?but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your?experience, hints etc, how i should perform my task and receive a good quality specimens. Thank you in advance. Galina Dunk, Novartis, Cambridge MA --- From RCazares <@t> schosp.org Wed Nov 18 12:35:19 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Nov 18 12:35:33 2009 Subject: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab - Email found in subject In-Reply-To: <001301ca6879$e167d3d0$0f01a8c0@biopath.local> References: <001301ca6879$e167d3d0$0f01a8c0@biopath.local> Message-ID: <572D1F45B44B3D4096D554B4CB40639C01F26FE996@EXCHCCRMB.schosp.org> Hi Paula, We did the same thing with our coverslipper, venting to the ceiling and out. We also had the stainer vented out through the ceiling and this helped dramatically. If you do vent your stainer though, you have to remove the charcoal filter, as the filter actually prevents the fumes from being sucked out efficiently. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, November 18, 2009 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab - Email found in subject Hello everyone, I'm hoping someone can help me. We have a small room in the lab where the coverslipper and stainer are. We have the coverslipper attached to a fume hood which is attached to the ceiling to exhaust the fumes out of the building. The stainer has a charcoal filter inside the unit. We have an air purifier to help with ventilation and we have a fume hood to work under as well. With all these precautions, this area still gets a good amount of fumes lurking around for people to feel uncomfortable with. We do the badge monitoring and are always under the limits, but I still would like to get it better. What kind of ventilation solutions have you done to help minimize the fumes and/or do you have a contact of a Certified Industrial Hygienist? I would like one to come and take measurements or monitor the fumes in this area. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 18 12:40:59 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Nov 18 12:41:30 2009 Subject: [Histonet] Re: Freezing of the sceletal muscles In-Reply-To: <219946.53957.qm@web33103.mail.mud.yahoo.com> References: <219946.53957.qm@web33103.mail.mud.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4CF77A850F@EXMBMCB15.ucsfmedicalcenter.org> Galina, Get a FLUKE 51-2 electronic thermometer, then get the thermocouple that detects below -100C. Thermocouple Temperature Measurement accuracy: Above -100 ?C (-148 ?F) J, K, T, E, and N-type** ?[0.05% + 0.3?C (0.5?F) ] R** and S-type** ?[0.05% + 0.4?C (0.7?F) ] Below -100 ?C (-148 ?F): J, K, E, and N-types ?[0.20% + 0.3?C (0.5?F) ] T-type ?[0.50% + 0.3?C (0.5?F) ] Display resolution: 0.1?C / ?F / K < 1000? 1?C / ?F / K ? 1000? Measurement range: J-type: -210?C to 1200?C (-346?F to 2192?F) K-type: -200?C to 1372?C (-328?F to 2501?F) T-type: -250?C to 400?C (-418?F to 752?F) E-type: -150?C to 1000?C (-238?F to 1832?F) N-type**: -200?C to 1300?C (-328?F to 2372?F) R** and S-type**: 0?C to 1767?C (32?F to 3212?F) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Wednesday, November 18, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Freezing of the sceletal muscles Dear Colleagues. I have couple questions regarding freezing of the mouse / rat muscles. 1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful. 2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining, ?but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your?experience, hints etc, how i should perform my task and receive a good quality specimens. Thank you in advance. Galina Dunk, Novartis, Cambridge MA --- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Nov 18 14:21:59 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 18 14:22:06 2009 Subject: [Histonet] OSHA shoe requirements for histology In-Reply-To: <714075.28027.qm@web36305.mail.mud.yahoo.com> Message-ID: <414701.30978.qm@web65714.mail.ac4.yahoo.com> That one and also not using open shoes. Ren? J. --- On Wed, 11/18/09, richard wells wrote: From: richard wells Subject: [Histonet] OSHA shoe requirements for histology To: "histonet" Date: Wednesday, November 18, 2009, 11:36 AM It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors.? Does anyone have any more information regarding this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Wed Nov 18 14:30:18 2009 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Nov 18 14:30:25 2009 Subject: [Histonet] IHC in Ontario Canada Message-ID: Hi Sheila, What are the Ontario Laboratory Accreditation requirements questions that you have regarding IHC? We were assessed in spring/09. We run well over a hundred markers using three Ventana BenchMark XT's. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From jbdg <@t> u.washington.edu Wed Nov 18 16:16:28 2009 From: jbdg <@t> u.washington.edu (Josephine Garcia) Date: Wed Nov 18 16:16:32 2009 Subject: [Histonet] Rat perfusion protocol- what's wrong? Message-ID: Hi all, We are trying to harvest muscle and nerve tissue from Sprague-Dawley rats (upper leg muscle, sciatic nerve). Is it necessary to perfuse the animal before harvesting muscle / nerve, or will simply soaking in 4% PFA do? Our perfusions often, but not always, fail. By "fail", I mean the rat does not twitch after Paraformaldehyde is circulated, and the tissue does not get stiff. Here is our protocol. ? Inject with 0.5 mL Beuthanasia (pink liquid, draw up with 3mL syringe) into liver (right abdomen) ? Open chest cavity on perfusion table by pulling skin on chest up and cutting away circular region. Pierce diaphragm, cut up through ribs (towards armpit) with blunt side of scissors down, exposing heart and lungs ? Inject butterfly needle (connected to pump) into the lobe that faces the back ? go up into the heart from the bottom (see diagram) ? Turn on perfusion pump (clockwise button) at ~20mL/min ? Cut (using smallest scissors available) black sac near the heart ? this is the vena cava. If done correctly, the cavity should fill with blood ? Pump > 60mL of saline from the syringe into the system, draining the blood. Once the blood being drained turns into a clearer red, switch to Paraformaldehyde ? Pump > 150mL of cold 4% Paraformaldehyde. The rat should twitch and stiffen within minutes if perfusion is successful (twitching occurs ~90% of the time) Also, if perfusion is successful, we soak the tissue in 0.4% paraformaldehyde instead of 4%. Is this correct? Thanks for the help. ** From jclark <@t> pcnm.com Wed Nov 18 18:29:58 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Nov 18 18:30:02 2009 Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 19 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01142EC9@mail.pcnm.com> Hi Shiela, I went through an OLA accreditation before I came to work here the States and it seems to me if you do all the same QC you did for CAP you will be OK. You will need to validate your instruments and all of your IHC protocols and have records to show. All of your procedures for all your antibodies will need to be documented and how you QC test them. Unless they have changed things, you can use outdated antibodies with OLA if you have a written procedure that outlines how they are used and what to do if the QC/QA guidelines are not met. You will need to have QC testing records for when a new shipment of antibody is received from the vendor before being put into use. Joanne Clark, HT, MLT(CSMLS) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM Message: 9 Date: Wed, 18 Nov 2009 07:35:11 -0500 From: sheila adey Subject: [Histonet] IHC in Ontario Canada To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, I've recently started working back in Canada and I am looking to find other techs that are familiar with the OLA regulations for IHCs. Seems to be a little different than the CAP guidelines. Sheila Adey HT MLT _________________________________________________________________ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819 ------------------------------ From AnthonyH <@t> chw.edu.au Wed Nov 18 19:01:20 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 18 19:01:33 2009 Subject: [Histonet] Fwd: foxp3 ab ihc In-Reply-To: <4B03C2C50200009000080256@gwiasmtp.uct.ac.za> Message-ID: Marilyn, We have used Biolegend's Rabbit anti FoxP3 (Cat No 623801) at a 1/20 dilution with citrate retrieval (ER1) on the Bond Max with very good results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Tyler Sent: Wednesday, 18 November 2009 6:48 PM To: histonet Subject: [Histonet] Fwd: foxp3 ab ihc Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From CIngles <@t> uwhealth.org Wed Nov 18 20:25:08 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Nov 18 20:25:31 2009 Subject: [Histonet] OSHA shoe requirements for histology References: <714075.28027.qm@web36305.mail.mud.yahoo.com> Message-ID: No open toed shoes for one. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of richard wells Sent: Wed 11/18/2009 10:36 AM To: histonet Subject: [Histonet] OSHA shoe requirements for histology It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors. Does anyone have any more information regarding this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Nov 18 20:25:59 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Nov 18 20:26:07 2009 Subject: [Histonet] Sanderson's Rapid Bone Stain and what you can do about it Message-ID: <000401ca68bf$a3360870$e9a21950$@callis@bresnan.net> It was good hear that RBS was being picked up and sold by Dorn and Hart, but you can also make this up yourself in the laboratory. Cathy Sanderson/Mayton found a way to make this up easier than the original formula and Surgipath manufactured it. The original name was Stevenels Blue and the original publication for staining PMMA embedded bone: Maniatopoulos C et al (1987) An improved method for preparing histological sections of metallic implants. International J or Oral and Maxillofacial Implants 1:(1):31 It can be made up in the laboratory although time consuming and messy. We devised a way to make it up as quickly as possible. I will be happy to send the recipe and preparation method to anyone who needs it privately. Cathy did us all a favor by making this up in some proprietary way so we could purchase it easily. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 From c.m.vanderloos <@t> amc.uva.nl Thu Nov 19 01:45:26 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Nov 19 01:45:40 2009 Subject: [Histonet] RE: foxp3 ab ihc Message-ID: Marilyn,Clone 236A/E7 (Abcam) worked fine for us. See: De Boer et al. (2007) PlosOne 8:e779 and De Boer et al. (2007) JHC 55:891-898.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Wed, 18 Nov 2009 09:47:49 +0200 From: "Marilyn Tyler" Subject: [Histonet] Fwd: foxp3 ab ihc To: "histonet" histonet@lists.utsouthwestern.edu Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather From alisha <@t> ka-recruiting.com Thu Nov 19 08:59:01 2009 From: alisha <@t> ka-recruiting.com (Alisha Taylor) Date: Thu Nov 19 08:58:52 2009 Subject: [Histonet] Histotech Jobs! Message-ID: <1137998437.1258642741372.JavaMail.cfservice@webserver59> Dear Histotechnologists, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a mid-sized nonprofit hospital in southwest Oklahoma. This area is known for its year round recreational activities as it's located near the mountains and a lake. My client is looking for histotech for their 1st shift position. The ideal candidate will have graduated from an accredited Histotechnology school, will be ASCP certified as an HT or HTL, and will have greater than 1 year of experience in a histology lab. My client offers a very competitive hourly rate, full benefits, and possible relocation assistance, if needed. Please email or call me if you have an interest in learning more about this position. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Other Current Histology Opportunities: Histology Supervisor - Georgia (1st shift) Histotechnologist- Georgia (1st shift) Histotechnologist - Southern CA (all shifts) Histology Supervisor- Southern CA (3rd shift) Pathology Manager - Bay Area, CA (1st shift) Histotechnologist - Boston, MA (1st shift) Histotechnologist - Oklahoma Histology Supervisor- Oklahoma (1st shift) Histotechnologist - New York City, NY (3rd shift) Pathology Manager - New York City, NY (1st shift) Histotechnologist - Otsego County, NY (Upstate NY) If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha R. Taylor, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From eileen_dusek <@t> yahoo.com Thu Nov 19 10:41:23 2009 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Thu Nov 19 10:41:26 2009 Subject: [Histonet] unscribe Message-ID: <80487.91368.qm@web50602.mail.re2.yahoo.com> From stamptrain <@t> yahoo.com Thu Nov 19 11:46:38 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Nov 19 11:46:41 2009 Subject: [Histonet] unscribe In-Reply-To: <80487.91368.qm@web50602.mail.re2.yahoo.com> References: <80487.91368.qm@web50602.mail.re2.yahoo.com> Message-ID: <798449.19125.qm@web55808.mail.re3.yahoo.com> First of all, that's not even a word.? Secondly, that's not the way it's done.? I'm sure others will point you in the right direction. Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: eileen dusek To: histonet@lists.utsouthwestern.edu Sent: Thu, November 19, 2009 11:41:23 AM Subject: [Histonet] unscribe ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Thu Nov 19 12:03:00 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Nov 19 12:03:12 2009 Subject: [Histonet] unscribe In-Reply-To: <798449.19125.qm@web55808.mail.re3.yahoo.com> References: <80487.91368.qm@web50602.mail.re2.yahoo.com> <798449.19125.qm@web55808.mail.re3.yahoo.com> Message-ID: <4B058854.7070203@umdnj.edu> I must admit... there is precedent... at least on this list of word-smiths ;) http://lists.utsouthwestern.edu/mailman/mmsearch/histonet Roger Moretz wrote: > First of all, that's not even a word. Secondly, that's not the way it's done. I'm sure others will point you in the right direction. > > Roger Moretz, Ph.D. (ret.) > > > > ----- Original Message ---- > From: eileen dusek > To: histonet@lists.utsouthwestern.edu > Sent: Thu, November 19, 2009 11:41:23 AM > Subject: [Histonet] unscribe > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From gmartin <@t> marshallmedical.org Thu Nov 19 12:08:59 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Nov 19 12:09:05 2009 Subject: [Histonet] !0% buffered formalin corrosive? Message-ID: <6ED9D4252F278841A0593D3D788AF24C06FBE202@mailsvr.MARSHMED.local> I have a question around 10% buffered formalin being shipped to us. The shipper considers this corrosive. I have been told that if the formalin is shipped in 6oml biopsy bottles it does not receive that "corrosive" rating when shipped. I have also been told that if the shipment of formalin is on a pallet and it weights 220 lbs or more, it will not receive the "corrosive" rating when shipped. Can someone bring some clarity to the "corrosive" rating for me. Much thanks Gary From JWeems <@t> sjha.org Thu Nov 19 12:09:23 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 19 12:09:28 2009 Subject: [Histonet] unscribe In-Reply-To: <4B058854.7070203@umdnj.edu> References: <80487.91368.qm@web50602.mail.re2.yahoo.com><798449.19125.qm@web55808.mail.re3.yahoo.com> <4B058854.7070203@umdnj.edu> Message-ID: Eileen, We must deal with this through the website now.. It's at the bottom of all emails as well. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From alyssa <@t> alliedsearchpartners.com Thu Nov 19 12:10:41 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Nov 19 12:10:53 2009 Subject: [Histonet] unscribe In-Reply-To: <4B058854.7070203@umdnj.edu> References: <80487.91368.qm@web50602.mail.re2.yahoo.com> <798449.19125.qm@web55808.mail.re3.yahoo.com> <4B058854.7070203@umdnj.edu> Message-ID: FYI: My colleague tried to unsubscribe and the button that you click, on the below link does not work.... On Thu, Nov 19, 2009 at 1:03 PM, Peter Carroll wrote: > I must admit... there is precedent... at least on this list of word-smiths > ;) > http://lists.utsouthwestern.edu/mailman/mmsearch/histonet > > > Roger Moretz wrote: > >> First of all, that's not even a word. Secondly, that's not the way it's >> done. I'm sure others will point you in the right direction. >> >> Roger Moretz, Ph.D. (ret.) >> >> >> >> ----- Original Message ---- >> From: eileen dusek >> To: histonet@lists.utsouthwestern.edu >> Sent: Thu, November 19, 2009 11:41:23 AM >> Subject: [Histonet] unscribe >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From carrolpb <@t> umdnj.edu Thu Nov 19 12:14:19 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Nov 19 12:14:27 2009 Subject: [Histonet] unscribe In-Reply-To: References: <80487.91368.qm@web50602.mail.re2.yahoo.com> <798449.19125.qm@web55808.mail.re3.yahoo.com> <4B058854.7070203@umdnj.edu> Message-ID: <4B058AFB.9080605@umdnj.edu> > FYI: My colleague tried to unsubscribe and the button that you click, on the below link does not work.... That was a broken link that was supposed to link to the archive search for "unscribe" ;) The link you want to click is this one, at the bottom of EVERY mail form the list: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Alyssa Peterson wrote: > FYI: My colleague tried to unsubscribe and the button that you click, > on the below link does not work.... > > On Thu, Nov 19, 2009 at 1:03 PM, Peter Carroll > wrote: > > I must admit... there is precedent... at least on this list of > word-smiths ;) > http://lists.utsouthwestern.edu/mailman/mmsearch/histonet > > > Roger Moretz wrote: > > First of all, that's not even a word. Secondly, that's not > the way it's done. I'm sure others will point you in the > right direction. > > Roger Moretz, Ph.D. (ret.) > > > > ----- Original Message ---- > From: eileen dusek > > To: histonet@lists.utsouthwestern.edu > > Sent: Thu, November 19, 2009 11:41:23 AM > Subject: [Histonet] unscribe > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > *Be sure to visit us on the web* www.alliedsearchpartners.com > > Alyssa Peterson, Director Of Recruitment > Allied Search Partners > O:888.388.7571 ext. 101 > F: 888.388.7572 From thomas.crowell <@t> novartis.com Thu Nov 19 12:19:44 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Nov 19 12:21:51 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 11/19/2009 and will not return until 11/20/2009. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From rsrichmond <@t> gmail.com Thu Nov 19 12:21:57 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Nov 19 12:22:00 2009 Subject: [Histonet] Re: freezing skeletal muscle Message-ID: Galina Deyneko asks about freezing skeletal muscle. I'm not sure that temperature control of the isopentane is too important - it should be very slightly viscous. But do get rid of explosive isopentane and get a non-flammable substitute - see my previous posts on this topic. The technique for freezing skeletal muscle is easier demonstrated than described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat it with talc powder so that it appears white on the surface. Then dip the specimen in and out of the isopentane - roughly two dips per second - until it is frozen solid. That will eliminate the ice crystal artifact. But you have to try it a few times before it will quite work for you. Set aside one dead rat and one afternoon. Bob Richmond Samurai Pathologist Knoxville TN From khavens <@t> stormontvail.org Thu Nov 19 12:43:18 2009 From: khavens <@t> stormontvail.org (Havens, Kimberley) Date: Thu Nov 19 12:43:47 2009 Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 20 In-Reply-To: <164d18030001a648@stormontvail.org> Message-ID: Concerning Paula Lucas's request for fumes in her lab: We have a ThermoScientific Hyperclean Fume Extraction Hood that sits on a table-ledge in our Mohs Lab. We have the Hyperclean Hood on the whole time we are processing the tissues. We have not had any problems with fumes. I think the investment of the Hyperclean Fume Hood is worthwhile in keeping the fumes under control. If you need more information concerning the Hyperclean Hood, you can go to www.thermo.com. I hope this answers your question. Have a great day!~ Kimberley Havens MOHS Technician -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, November 19, 2009 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 72, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Industrial Hygienist/Fumes in lab (Paula Lucas) 2. Re: Freezing of the sceletal muscles (Galina Deyneko) 3. RE: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab - Email found in subject (Cazares, Ruth) 4. RE: Re: Freezing of the sceletal muscles (Morken, Tim) 5. Re: OSHA shoe requirements for histology (Rene J Buesa) 6. IHC in Ontario Canada (Gagnon, Eric) 7. Rat perfusion protocol- what's wrong? (Josephine Garcia) 8. RE: Histonet Digest, Vol 72, Issue 19 (Joanne Clark) 9. RE: Fwd: foxp3 ab ihc (Tony Henwood) 10. RE: OSHA shoe requirements for histology (Ingles Claire ) 11. Sanderson's Rapid Bone Stain and what you can do about it (gayle callis) 12. RE: foxp3 ab ihc (C.M. van der Loos) 13. Histotech Jobs! (Alisha Taylor) 14. unscribe (eileen dusek) 15. Re: unscribe (Roger Moretz) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Nov 2009 10:06:41 -0800 From: "Paula Lucas" Subject: [Histonet] Industrial Hygienist/Fumes in lab To: Message-ID: <001301ca6879$e167d3d0$0f01a8c0@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello everyone, I'm hoping someone can help me. We have a small room in the lab where the coverslipper and stainer are. We have the coverslipper attached to a fume hood which is attached to the ceiling to exhaust the fumes out of the building. The stainer has a charcoal filter inside the unit. We have an air purifier to help with ventilation and we have a fume hood to work under as well. With all these precautions, this area still gets a good amount of fumes lurking around for people to feel uncomfortable with. We do the badge monitoring and are always under the limits, but I still would like to get it better. What kind of ventilation solutions have you done to help minimize the fumes and/or do you have a contact of a Certified Industrial Hygienist? I would like one to come and take measurements or monitor the fumes in this area. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group ------------------------------ Message: 2 Date: Wed, 18 Nov 2009 10:13:32 -0800 (PST) From: Galina Deyneko Subject: [Histonet] Re: Freezing of the sceletal muscles To: histonet@lists.utsouthwestern.edu Message-ID: <219946.53957.qm@web33103.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Colleagues. I have couple questions regarding freezing of the mouse / rat muscles. 1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful. 2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining, ?but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your?experience, hints etc, how i should perform my task and receive a good quality specimens. Thank you in advance. Galina Dunk, Novartis, Cambridge MA --- ------------------------------ Message: 3 Date: Wed, 18 Nov 2009 12:35:19 -0600 From: "Cazares, Ruth" Subject: RE: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab - Email found in subject To: Paula Lucas , "histonet@lists.utsouthwestern.edu" Message-ID: <572D1F45B44B3D4096D554B4CB40639C01F26FE996@EXCHCCRMB.schosp.org> Content-Type: text/plain; charset="us-ascii" Hi Paula, We did the same thing with our coverslipper, venting to the ceiling and out. We also had the stainer vented out through the ceiling and this helped dramatically. If you do vent your stainer though, you have to remove the charcoal filter, as the filter actually prevents the fumes from being sucked out efficiently. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, November 18, 2009 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab - Email found in subject Hello everyone, I'm hoping someone can help me. We have a small room in the lab where the coverslipper and stainer are. We have the coverslipper attached to a fume hood which is attached to the ceiling to exhaust the fumes out of the building. The stainer has a charcoal filter inside the unit. We have an air purifier to help with ventilation and we have a fume hood to work under as well. With all these precautions, this area still gets a good amount of fumes lurking around for people to feel uncomfortable with. We do the badge monitoring and are always under the limits, but I still would like to get it better. What kind of ventilation solutions have you done to help minimize the fumes and/or do you have a contact of a Certified Industrial Hygienist? I would like one to come and take measurements or monitor the fumes in this area. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ------------------------------ Message: 4 Date: Wed, 18 Nov 2009 10:40:59 -0800 From: "Morken, Tim" Subject: RE: [Histonet] Re: Freezing of the sceletal muscles To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF77A850F@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=iso-8859-1 Galina, Get a FLUKE 51-2 electronic thermometer, then get the thermocouple that detects below -100C. Thermocouple Temperature Measurement accuracy: Above -100 ?C (-148 ?F) J, K, T, E, and N-type** ?[0.05% + 0.3?C (0.5?F) ] R** and S-type** ?[0.05% + 0.4?C (0.7?F) ] Below -100 ?C (-148 ?F): J, K, E, and N-types ?[0.20% + 0.3?C (0.5?F) ] T-type ?[0.50% + 0.3?C (0.5?F) ] Display resolution: 0.1?C / ?F / K < 1000? 1?C / ?F / K ? 1000? Measurement range: J-type: -210?C to 1200?C (-346?F to 2192?F) K-type: -200?C to 1372?C (-328?F to 2501?F) T-type: -250?C to 400?C (-418?F to 752?F) E-type: -150?C to 1000?C (-238?F to 1832?F) N-type**: -200?C to 1300?C (-328?F to 2372?F) R** and S-type**: 0?C to 1767?C (32?F to 3212?F) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Wednesday, November 18, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Freezing of the sceletal muscles Dear Colleagues. I have couple questions regarding freezing of the mouse / rat muscles. 1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful. 2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining, ?but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your?experience, hints etc, how i should perform my task and receive a good quality specimens. Thank you in advance. Galina Dunk, Novartis, Cambridge MA --- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 18 Nov 2009 12:21:59 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] OSHA shoe requirements for histology To: histonet , richard wells Message-ID: <414701.30978.qm@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 That one and also not using open shoes. Ren? J. --- On Wed, 11/18/09, richard wells wrote: From: richard wells Subject: [Histonet] OSHA shoe requirements for histology To: "histonet" Date: Wednesday, November 18, 2009, 11:36 AM It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors.? Does anyone have any more information regarding this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 18 Nov 2009 15:30:18 -0500 From: "Gagnon, Eric" Subject: [Histonet] IHC in Ontario Canada To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Sheila, What are the Ontario Laboratory Accreditation requirements questions that you have regarding IHC? We were assessed in spring/09. We run well over a hundred markers using three Ventana BenchMark XT's. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ------------------------------ Message: 7 Date: Wed, 18 Nov 2009 14:16:28 -0800 From: Josephine Garcia Subject: [Histonet] Rat perfusion protocol- what's wrong? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Hi all, We are trying to harvest muscle and nerve tissue from Sprague-Dawley rats (upper leg muscle, sciatic nerve). Is it necessary to perfuse the animal before harvesting muscle / nerve, or will simply soaking in 4% PFA do? Our perfusions often, but not always, fail. By "fail", I mean the rat does not twitch after Paraformaldehyde is circulated, and the tissue does not get stiff. Here is our protocol. ? Inject with 0.5 mL Beuthanasia (pink liquid, draw up with 3mL syringe) into liver (right abdomen) ? Open chest cavity on perfusion table by pulling skin on chest up and cutting away circular region. Pierce diaphragm, cut up through ribs (towards armpit) with blunt side of scissors down, exposing heart and lungs ? Inject butterfly needle (connected to pump) into the lobe that faces the back - go up into the heart from the bottom (see diagram) ? Turn on perfusion pump (clockwise button) at ~20mL/min ? Cut (using smallest scissors available) black sac near the heart - this is the vena cava. If done correctly, the cavity should fill with blood ? Pump > 60mL of saline from the syringe into the system, draining the blood. Once the blood being drained turns into a clearer red, switch to Paraformaldehyde ? Pump > 150mL of cold 4% Paraformaldehyde. The rat should twitch and stiffen within minutes if perfusion is successful (twitching occurs ~90% of the time) Also, if perfusion is successful, we soak the tissue in 0.4% paraformaldehyde instead of 4%. Is this correct? Thanks for the help. ** ------------------------------ Message: 8 Date: Wed, 18 Nov 2009 17:29:58 -0700 From: "Joanne Clark" Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 19 To: Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01142EC9@mail.pcnm.com> Content-Type: text/plain; charset="US-ASCII" Hi Shiela, I went through an OLA accreditation before I came to work here the States and it seems to me if you do all the same QC you did for CAP you will be OK. You will need to validate your instruments and all of your IHC protocols and have records to show. All of your procedures for all your antibodies will need to be documented and how you QC test them. Unless they have changed things, you can use outdated antibodies with OLA if you have a written procedure that outlines how they are used and what to do if the QC/QA guidelines are not met. You will need to have QC testing records for when a new shipment of antibody is received from the vendor before being put into use. Joanne Clark, HT, MLT(CSMLS) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM Message: 9 Date: Wed, 18 Nov 2009 07:35:11 -0500 From: sheila adey Subject: [Histonet] IHC in Ontario Canada To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, I've recently started working back in Canada and I am looking to find other techs that are familiar with the OLA regulations for IHCs. Seems to be a little different than the CAP guidelines. Sheila Adey HT MLT _________________________________________________________________ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819 ------------------------------ ------------------------------ Message: 9 Date: Thu, 19 Nov 2009 12:01:20 +1100 From: "Tony Henwood" Subject: RE: [Histonet] Fwd: foxp3 ab ihc To: "Marilyn Tyler" , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Marilyn, We have used Biolegend's Rabbit anti FoxP3 (Cat No 623801) at a 1/20 dilution with citrate retrieval (ER1) on the Bond Max with very good results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Tyler Sent: Wednesday, 18 November 2009 6:48 PM To: histonet Subject: [Histonet] Fwd: foxp3 ab ihc Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 10 Date: Wed, 18 Nov 2009 20:25:08 -0600 From: "Ingles Claire " Subject: RE: [Histonet] OSHA shoe requirements for histology To: "histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" No open toed shoes for one. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of richard wells Sent: Wed 11/18/2009 10:36 AM To: histonet Subject: [Histonet] OSHA shoe requirements for histology It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors. Does anyone have any more information regarding this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 18 Nov 2009 19:25:59 -0700 From: "gayle callis" Subject: [Histonet] Sanderson's Rapid Bone Stain and what you can do about it To: "'Histonet'" Message-ID: <000401ca68bf$a3360870$e9a21950$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" It was good hear that RBS was being picked up and sold by Dorn and Hart, but you can also make this up yourself in the laboratory. Cathy Sanderson/Mayton found a way to make this up easier than the original formula and Surgipath manufactured it. The original name was Stevenels Blue and the original publication for staining PMMA embedded bone: Maniatopoulos C et al (1987) An improved method for preparing histological sections of metallic implants. International J or Oral and Maxillofacial Implants 1:(1):31 It can be made up in the laboratory although time consuming and messy. We devised a way to make it up as quickly as possible. I will be happy to send the recipe and preparation method to anyone who needs it privately. Cathy did us all a favor by making this up in some proprietary way so we could purchase it easily. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ------------------------------ Message: 12 Date: Thu, 19 Nov 2009 08:45:26 +0100 From: "C.M. van der Loos" Subject: [Histonet] RE: foxp3 ab ihc To: Marilyn.Tyler@uct.ac.za Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Marilyn,Clone 236A/E7 (Abcam) worked fine for us. See: De Boer et al. (2007) PlosOne 8:e779 and De Boer et al. (2007) JHC 55:891-898.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Wed, 18 Nov 2009 09:47:49 +0200 From: "Marilyn Tyler" Subject: [Histonet] Fwd: foxp3 ab ihc To: "histonet" histonet@lists.utsouthwestern.edu Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather ------------------------------ Message: 13 Date: 19 Nov 2009 09:59:01 -0500 From: Alisha Taylor Subject: [Histonet] Histotech Jobs! To: histonet@lists.utsouthwestern.edu Message-ID: <1137998437.1258642741372.JavaMail.cfservice@webserver59> Content-Type: text/plain; charset="utf-8" Dear Histotechnologists, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a mid-sized nonprofit hospital in southwest Oklahoma. This area is known for its year round recreational activities as it's located near the mountains and a lake. My client is looking for histotech for their 1st shift position. The ideal candidate will have graduated from an accredited Histotechnology school, will be ASCP certified as an HT or HTL, and will have greater than 1 year of experience in a histology lab. My client offers a very competitive hourly rate, full benefits, and possible relocation assistance, if needed. Please email or call me if you have an interest in learning more about this position. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Other Current Histology Opportunities: Histology Supervisor - Georgia (1st shift) Histotechnologist- Georgia (1st shift) Histotechnologist - Southern CA (all shifts) Histology Supervisor- Southern CA (3rd shift) Pathology Manager - Bay Area, CA (1st shift) Histotechnologist - Boston, MA (1st shift) Histotechnologist - Oklahoma Histology Supervisor- Oklahoma (1st shift) Histotechnologist - New York City, NY (3rd shift) Pathology Manager - New York City, NY (1st shift) Histotechnologist - Otsego County, NY (Upstate NY) If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha R. Taylor, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 14 Date: Thu, 19 Nov 2009 08:41:23 -0800 (PST) From: eileen dusek Subject: [Histonet] unscribe To: histonet@lists.utsouthwestern.edu Message-ID: <80487.91368.qm@web50602.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii ------------------------------ Message: 15 Date: Thu, 19 Nov 2009 09:46:38 -0800 (PST) From: Roger Moretz Subject: Re: [Histonet] unscribe To: eileen dusek , histonet@lists.utsouthwestern.edu Message-ID: <798449.19125.qm@web55808.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 First of all, that's not even a word.? Secondly, that's not the way it's done.? I'm sure others will point you in the right direction. Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: eileen dusek To: histonet@lists.utsouthwestern.edu Sent: Thu, November 19, 2009 11:41:23 AM Subject: [Histonet] unscribe ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 20 **************************************** Stormont-Vail HealthCare is proud to be in the top 5 percent of hospitals in the country honored by the American Nurses Credentialing Center's Magnet Recognition Program. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From BMolinari <@t> heart.thi.tmc.edu Thu Nov 19 13:00:01 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Nov 19 13:00:37 2009 Subject: [Histonet] Leica RM2165 instruction handbook Message-ID: Hi, Does anyone have a handbook for this microtome that they would be willing to fax to me? We inherited one with no instruction book. I have searched online and cannot find it. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From stevejohnson.pathquest <@t> yahoo.com Thu Nov 19 13:10:16 2009 From: stevejohnson.pathquest <@t> yahoo.com (Steve Johnson) Date: Thu Nov 19 13:10:35 2009 Subject: [Histonet] IHC tests in house vs. sending out Message-ID: <720463.53961.qm@web112502.mail.gq1.yahoo.com> Hi Histonet, ? I am trying to control which IHC tests I run in our lab vs. which tests I send out for.? My pathologists are interested in some new tests but?I am not sure how frequently they are going to be ordering them.? I'd rather do them in house if I can, but I don't want to be throwing my money away if they never order them... ? Does anyone know of a good system for keeping track of this? ? -Steve From trathborne <@t> somerset-healthcare.com Thu Nov 19 13:27:37 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Nov 19 13:27:43 2009 Subject: [Histonet] IHC tests in house vs. sending out In-Reply-To: <720463.53961.qm@web112502.mail.gq1.yahoo.com> Message-ID: First you need to determine which tests they have been requesting, and at what frequency. Evaluate the need over several months for each antibody. If it is ordered frequently enough to be cost effective and the pathologists will maintain competency, then you should consider bringing it in. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Steve Johnson Sent: Thursday, November 19, 2009 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC tests in house vs. sending out Hi Histonet, ? I am trying to control which IHC tests I run in our lab vs. which tests I send out for.? My pathologists are interested in some new tests but?I am not sure how frequently they are going to be ordering them.? I'd rather do them in house if I can, but I don't want to be throwing my money away if they never order them... ? Does anyone know of a good system for keeping track of this? ? -Steve _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. 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Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From gayle.callis <@t> bresnan.net Thu Nov 19 13:36:50 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Nov 19 13:37:01 2009 Subject: FW: [Histonet] Re: freezing skeletal muscle Message-ID: <000401ca694f$a541db90$efc592b0$@callis@bresnan.net> We always visually judged the correct temperature for LN2 cooled isopentane, by using a glass beaker, although as long as you use a container to see the following described appearance of isopentane aka 2 methylbutane when cooled, it will work. We lowered a glass beaker (containing isopentane) hanging from a wire holder (redesigned coat hanger!) into a Dewar of liquid nitrogen. When the isopentane becomes thick e.g. viscous, like Karo syrup or molasses, and the bottom of container had isopentane looking a bit white, the temperature was reached ~minus 120C. If we tried to test the temperature with a RT warm thermometer designed for cold temperatures, the isopentane immediately becames less viscous, and the temperature skewed towards warm. Doing this visually was much better than using a thermometer. In fact, if one cools isopentane to the point of becoming a solid aka a glass or super-cooled liquid, you can stir the isopentane with a long, RT metal forceps to let solvent go back to liquid and start over. As for isopentane, storage in an explosion proof refrigerator or freezer is mandatory, or store at room temperature if room is not too hot, in a flammable storage cabinet. This is what we have done in the past. It pays to be safe, and also use a fume hood to not breathe fumes from this solvent. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, November 19, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: freezing skeletal muscle Galina Deyneko asks about freezing skeletal muscle. I'm not sure that temperature control of the isopentane is too important - it should be very slightly viscous. But do get rid of explosive isopentane and get a non-flammable substitute - see my previous posts on this topic. The technique for freezing skeletal muscle is easier demonstrated than described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat it with talc powder so that it appears white on the surface. Then dip the specimen in and out of the isopentane - roughly two dips per second - until it is frozen solid. That will eliminate the ice crystal artifact. But you have to try it a few times before it will quite work for you. Set aside one dead rat and one afternoon. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4623 (20091119) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4623 (20091119) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4623 (20091119) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4623 (20091119) __________ The message was checked by ESET Smart Security. http://www.eset.com From LINDA.MARGRAF <@t> childrens.com Thu Nov 19 13:47:28 2009 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Nov 19 13:56:47 2009 Subject: [Histonet] unscribe In-Reply-To: References: <80487.91368.qm@web50602.mail.re2.yahoo.com> <798449.19125.qm@web55808.mail.re3.yahoo.com> <4B058854.7070203@umdnj.edu> Message-ID: <4B054C6F.F783.00DA.0@childrens.com> Dear Histonetters: I checked and the unsubscribe function on the web page is functional. I just removed myself from the list (but am getting back on). I will remove the person who inquired about "unscribing". If anyone else is having trouble getting off the list, please email me. If your address has changed since you first subscribed you may have trouble getting off the list. Thanks Linda M Histonet administrator >>> Alyssa Peterson 11/19/2009 12:10 PM >>> FYI: My colleague tried to unsubscribe and the button that you click, on the below link does not work.... On Thu, Nov 19, 2009 at 1:03 PM, Peter Carroll wrote: > I must admit... there is precedent... at least on this list of word-smiths > ;) > http://lists.utsouthwestern.edu/mailman/mmsearch/histonet > > > Roger Moretz wrote: > >> First of all, that's not even a word. Secondly, that's not the way it's >> done. I'm sure others will point you in the right direction. >> >> Roger Moretz, Ph.D. (ret.) >> >> >> >> ----- Original Message ---- >> From: eileen dusek >> To: histonet@lists.utsouthwestern.edu >> Sent: Thu, November 19, 2009 11:41:23 AM >> Subject: [Histonet] unscribe >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail

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From LSebree <@t> uwhealth.org Thu Nov 19 15:31:47 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Nov 19 15:31:52 2009 Subject: [Histonet] IHC tests in house vs. sending out In-Reply-To: <720463.53961.qm@web112502.mail.gq1.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF66F@UWHC-MAIL01.uwhis.hosp.wisc.edu> Steve, Our threshold is 12 tests/year for bringing on a new antibody in-house. On the flip side, if ordering volumes fall below 12/year, we consider dropping the antibody. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steve Johnson Sent: Thursday, November 19, 2009 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC tests in house vs. sending out Hi Histonet, ? I am trying to control which IHC tests I run in our lab vs. which tests I send out for.? My pathologists are interested in some new tests but?I am not sure how frequently they are going to be ordering them.? I'd rather do them in house if I can, but I don't want to be throwing my money away if they never order them... ? Does anyone know of a good system for keeping track of this? ? -Steve _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Thu Nov 19 15:38:42 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Nov 19 15:38:49 2009 Subject: [Histonet] xylen fumes for coverslipper resppnse Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13C37423@MD1EV002.medimmune.com> The only thing I can think of is perhaps you have a positive or negative pressure in the room that needs to be fixed. If you have competing drafts, or fans running, hoods pulling one way etc there could be a problem. Perhaps you could get an EHS group to do some air flow monitoring. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From MadaryJ <@t> MedImmune.com Thu Nov 19 15:41:54 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Nov 19 15:42:01 2009 Subject: [Histonet] fibrin control Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13C37430@MD1EV002.medimmune.com> Is there a naturally occurring fibrin/fibrinogen in mouse tissue? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From AnthonyH <@t> chw.edu.au Thu Nov 19 15:44:03 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Nov 19 15:44:11 2009 Subject: [Histonet] !0% buffered formalin corrosive? In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C06FBE202@mailsvr.MARSHMED.local> Message-ID: I would consider formalin corrosive regardless of the form it is in. It is cytotoxic. That is why it kills and preserves cells and tissues so well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Friday, 20 November 2009 5:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] !0% buffered formalin corrosive? I have a question around 10% buffered formalin being shipped to us. The shipper considers this corrosive. I have been told that if the formalin is shipped in 6oml biopsy bottles it does not receive that "corrosive" rating when shipped. I have also been told that if the shipment of formalin is on a pallet and it weights 220 lbs or more, it will not receive the "corrosive" rating when shipped. Can someone bring some clarity to the "corrosive" rating for me. Much thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From cliffberger <@t> mac.com Thu Nov 19 16:26:54 2009 From: cliffberger <@t> mac.com (Cliff Berger) Date: Thu Nov 19 16:27:01 2009 Subject: [Histonet] !0% buffered formalin corrosive? In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C06FBE202@mailsvr.MARSHMED.local> Message-ID: 10% Neutral Buffered Formalin, which is probably what most histology labs are using, is not corrosive and it has no hazardous designation what so ever as far as commercial shipping in the United States is concerned. The pH of this product should be between 6.8 and 7.1, hence the neutral designation. Formaldehyde, (which is sometimes referred to as Formalin) is hazardous and ships as a flammable and a corrosive liquid. -- Best regards! Cliff Berger Decal Chemical Corp 1-800-428-5856 On 11/19/09 1:08 PM, "Martin, Gary" wrote: > I have a question around 10% buffered formalin being shipped to us. The > shipper considers this corrosive. I have been told that if the formalin > is shipped in 6oml biopsy bottles it does not receive that "corrosive" > rating when shipped. I have also been told that if the shipment of > formalin is on a pallet and it weights 220 lbs or more, it will not > receive the "corrosive" rating when shipped. Can someone bring some > clarity to the "corrosive" rating for me. > > Much thanks > > Gary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Nov 19 17:11:37 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Nov 19 17:11:47 2009 Subject: [Histonet] Second Call for Manuscripts for Special Issue of Journal of Histotechnology Message-ID: <002301ca696d$a65f0fc0$f31d2f40$@callis@bresnan.net> Dear Histonet Colleagues, A second call for manuscripts for Special Issue of Journal of Histotechnology: The Journal of Histotechnology (JOH) editors, Editorial Board, and guest editor Dr. Richard Cartun are pleased to announce a call for manuscripts for the March 2010 special issue of JOH, with focus on Immunohistochemistry and In Situ Hybridization. Technical notes and methodology-focused contributions are particularly encouraged; clinical, research, and veterinary topics are welcome. Manuscripts must be submitted prior to November 30, 2009 in order to be considered for the March issue. Manuscripts may be sent to Ms. Jane Jacobi, Managing Editor, at joh@nsh.org. Authors wishing pre-submission guidance with the writing process and assignment of a writing partner may contact Ms. Gayle Callis, JOH Assistant Editor, at gayle.callis@bresnan.net. Information for authors is detailed at www.nsh.org under Journal of Histotechnology. and an example of a Technical Note format is available upon request from Ms. Callis and NSH office. Gayle M. Callis JOH Assistant Editor National Society for Histotechnology Journal of Histotechnology 10320 Little Patuxent Pkwy #804 Columbia, MD 21044 P: 443-535-4060 E: joh@nsh.org From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 19 18:20:16 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Nov 19 18:20:41 2009 Subject: [Histonet] Preventing slide labeling mistakes Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892093@EXMBMCB15.ucsfmedicalcenter.org> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA From lpaveli1 <@t> hurleymc.com Thu Nov 19 21:52:07 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Nov 19 21:52:25 2009 Subject: [Histonet] Preventing slide labeling mistakes Message-ID: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.Tyler <@t> uct.ac.za Thu Nov 19 23:52:05 2009 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Thu Nov 19 23:52:16 2009 Subject: [Histonet] Fwd: foxp3 ab ihc Message-ID: <4B064AA5020000900008096B@gwiasmtp.uct.ac.za> Thanks to all in Histonet world posted on Heathers behalf who is still having problems with her access Marilyn >>> Heather McCleod 2009/11/19 10:43 AM >>> Sorry to bother you Marilyn. Please forward. I have an answer, i.e. confirmation of what I would like to use. Thanks to all who replied to my foxp3 query. Heather >>> "Marilyn Tyler" 2009/11/18 09:47 AM >>> Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Nov 20 08:37:53 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Nov 20 08:37:57 2009 Subject: [Histonet] Preventing slide labeling mistakes In-Reply-To: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CA2@is-e2k3.grhs.net> You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 20 08:52:54 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 20 08:53:38 2009 Subject: SPAM-LOW: [Histonet] Re: freezing skeletal muscle In-Reply-To: References: Message-ID: Especially for bone, I have found that formalin fixation and then infiltrating in 30% sucrose before freezing the way Samuri described works the very best for me. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, November 19, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: freezing skeletal muscle Galina Deyneko asks about freezing skeletal muscle. I'm not sure that temperature control of the isopentane is too important - it should be very slightly viscous. But do get rid of explosive isopentane and get a non-flammable substitute - see my previous posts on this topic. The technique for freezing skeletal muscle is easier demonstrated than described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat it with talc powder so that it appears white on the surface. Then dip the specimen in and out of the isopentane - roughly two dips per second - until it is frozen solid. That will eliminate the ice crystal artifact. But you have to try it a few times before it will quite work for you. Set aside one dead rat and one afternoon. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Fri Nov 20 09:10:36 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Nov 20 09:11:07 2009 Subject: [Histonet] Preventing slide labeling mistakes In-Reply-To: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> References: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> Message-ID: <4B06B16C.2000507@pathology.washington.edu> Lynette, You might want to also investigate different fonts if that is an option. Keeping the same size but using a different font can make a world of difference. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Lynette Pavelich wrote: > Hi Tim, > We do everything you do, but we also match our blocks to the written > number on the slides. We catch the majority of our mistakes at that > point. The other is because the font on the label is so small, we have > a hard time seeing the difference between 5's & 6's!!! I'm sure it's > not our ages or anything! We're working with CoPath to get a larger > font!! Ahhh, getting old is SO fun! And yes, reading glasses work > pretty good!! LOL > > Have a great weekend everyone!! > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > MSH Competency Coordinator > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > email: Lpaveli1@hurleymc.com > ph: 810-257-9948 > fax: 810-762-7082 > >>>> "Morken, Tim" 11/19/09 7:20 PM >>> >>>> > Hi, Can people share their procedures for preventing manual slide > labeling mistakes? No need to include barcoding - we are exploring that > but it is a ways off. > > We currently have a manual process: > We prohibit pre-labeling slides in batches (many blocks/slides at one > time), require labeling slides (hand-written) only at the time of > cutting a single block, and matching paper label to the slide after > staining. We don't currently match blocks to slides. > > Thanks for any info! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 20 10:41:47 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Nov 20 10:42:00 2009 Subject: [Histonet] Preventing slide labeling mistakes In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3CA2@is-e2k3.grhs.net> References: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> <661949901A768E4F9CC16D8AF8F2838C017A3CA2@is-e2k3.grhs.net> Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Nov 20 10:55:04 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Nov 20 10:55:10 2009 Subject: [Histonet] Preventing slide labeling mistakes In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF677@UWHC-MAIL01.uwhis.hosp.wisc.edu> If the matching is done at the time of sectioning, i.e. making sure the block you are about to cut matches the slide you have ready to pick up that block's section(s), it will reduce mislabeling errors....and its GLP! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 10:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Arlene.Armandi <@t> cshs.org Fri Nov 20 11:03:08 2009 From: Arlene.Armandi <@t> cshs.org (Armandi, Arlene) Date: Fri Nov 20 11:03:15 2009 Subject: [Histonet] Preventing slide labeling mistakes In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: One more suggestion. Have you considered using Xylene resistant slide labels? We print the labels at the time we put the blocks in order. We then put the corresponding labels on the trays with the blocks. When the tech picks up a tray, they have the labels ready to put directly onto the slides as they cut. This eliminates the labeling errors resulting from the matching the hand written slides (sometimes illegible) to the labels, after the slides have been stained. Just a thought. Arlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 8:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From tfountain <@t> exchange.hsc.mb.ca Fri Nov 20 11:14:23 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Fri Nov 20 11:14:28 2009 Subject: [Histonet] CAP guidelines for positive controls Message-ID: Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From AFoshey <@t> chw.org Fri Nov 20 11:54:59 2009 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Fri Nov 20 11:55:05 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports Message-ID: What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org From mtighe <@t> trudeauinstitute.org Fri Nov 20 11:57:36 2009 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Fri Nov 20 11:57:47 2009 Subject: [Histonet] course recommendation Message-ID: <4B069246.26E4.00EE.0@trudeauinstitute.org> I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike From rjbuesa <@t> yahoo.com Fri Nov 20 11:58:17 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 20 11:58:22 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports In-Reply-To: Message-ID: <654236.83215.qm@web65704.mail.ac4.yahoo.com> We used to add that the methods were experimental. Ren? J. --- On Fri, 11/20/09, Foshey, Annette wrote: From: Foshey, Annette Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Date: Friday, November 20, 2009, 12:54 PM What is the current practice for meeting this regulation?? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580? ? ? ? Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Nov 20 11:59:49 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 20 11:59:58 2009 Subject: AW: [Histonet] Preventing slide labeling mistakes In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF7892093@EXMBMCB15.ucsfmedicalcenter.org> References: <1AAF670737F193429070841C6B2ADD4CF7892093@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: We write a "macro-protocol" at the time of grossing with all blocknumbers and additional identifer. This paper is matched the first time with all blocks after embedding and before cutting. Missing blocks or wrong labelled blocks are found. The second time the paper is matched with all slides after staining and before delivering. Missing and wrong labelled slides are found. Additional the slide-number and material is matched with the "case sheet". The slides are labelled batchwise with 10 per batch immediatly before cutting. The identifiers (letters, "lateral", "resection-margin", etc.) are written on side of the cassette, so numbers can be written big enough. The numbers have maximum of 5 digits slash year. 12345/09 Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Morken, Tim Gesendet: Freitag, 20. November 2009 01:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Preventing slide labeling mistakes Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Nov 20 12:00:53 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Nov 20 12:00:57 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports In-Reply-To: References: Message-ID: We add ours to only the Class I ASR cases. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 12:55 To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Fri Nov 20 12:01:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 20 12:01:51 2009 Subject: [Histonet] CAP guidelines for positive controls In-Reply-To: Message-ID: <15262.94431.qm@web65702.mail.ac4.yahoo.com> They should be kept?to comply with?your policies and the state requirements. Remember that CAP has regulations but cannot override state nor institutional policies. Ren? J. --- On Fri, 11/20/09, Tiana Fountain wrote: From: Tiana Fountain Subject: [Histonet] CAP guidelines for positive controls To: histonet@lists.utsouthwestern.edu Date: Friday, November 20, 2009, 12:14 PM Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana -----Inline Attachment Follows----- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information.? Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.? If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Fri Nov 20 12:16:07 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Nov 20 12:16:12 2009 Subject: [Histonet] course recommendation In-Reply-To: <4B069246.26E4.00EE.0@trudeauinstitute.org> References: <4B069246.26E4.00EE.0@trudeauinstitute.org> Message-ID: Take a look at the National Society for Histotechnology (www.nsh.org). There are several State and Regional Society meetings coming up in the spring of 2010 and then of course there is the National Symposium/Convention in the Fall of 2010 in Seattle, WA. Jack Ratliff NSH -Hard Tissue Committee Chair > Date: Fri, 20 Nov 2009 12:57:36 -0500 > From: mtighe@trudeauinstitute.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] course recommendation > > > I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) > > Thanks for any suggestions!! > Mike > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Fri Nov 20 12:17:01 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Nov 20 12:17:05 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports In-Reply-To: References: Message-ID: <61135F0455D33347B5AAE209B903A304316E3E3E@EXCHVS2.medctr.ad.wfubmc.edu> We use the statement for all reports that any IHC/ISH, regardless if they are ASRs or not. Martha Ward Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 12:55 PM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 20 12:16:53 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 20 12:17:41 2009 Subject: SPAM-LOW: [Histonet] course recommendation In-Reply-To: <4B069246.26E4.00EE.0@trudeauinstitute.org> References: <4B069246.26E4.00EE.0@trudeauinstitute.org> Message-ID: Check out this Applied Immunohistochemistry and Molecular Pathology (AIMP) meeting coming at end of January 2009 in Florida. the fourth annual international retreat on applied IHC and molecular pathology. Please visit http://www.pathlearning.com/Pathology_Learning_Centers/Welcome.html for detailed information of the program and how to register. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Friday, November 20, 2009 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] course recommendation I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 20 12:18:42 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Nov 20 12:18:50 2009 Subject: [Histonet] Preventing slide labeling mistakes In-Reply-To: <8C023B4AB999614BA4791BAEB26E27381BF677@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> <8C023B4AB999614BA4791BAEB26E27381BF677@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892229@EXMBMCB15.ucsfmedicalcenter.org> Linda, Yes, I should have said "matching blocks and slides after staining catches mistakes but does not eliminate them." We are looking at: isolating block to be cut, labeling slide just before or after sectioning, matching block to slide after sectioning at the cutting station. Unfortunately with a manual method it comes to taking a lot of care with what one does up front. No amount of error checking will catch all possible mistakes, and can actually lead to more if it is too tedious and tiring. Tim -----Original Message----- From: Sebree Linda A [mailto:LSebree@uwhealth.org] Sent: Friday, November 20, 2009 8:55 AM To: Morken, Tim; Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes If the matching is done at the time of sectioning, i.e. making sure the block you are about to cut matches the slide you have ready to pick up that block's section(s), it will reduce mislabeling errors....and its GLP! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 10:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ritta69 <@t> iwon.com Fri Nov 20 12:19:28 2009 From: ritta69 <@t> iwon.com (ritta69.iwon) Date: Fri Nov 20 12:19:31 2009 Subject: [Histonet] frozen sections not adhering to slide Message-ID: <20091120131928.653@web010.roc2.bluetie.com> I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose until the brains sink and flash freeze in isopentane. All seems like pretty standard stuff. After cutting 45 um thick sections and storing them in antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene Glycol), I mount them on slides. After letting them dry overnight, I rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to wrinkle up and start coming of the slide during this first step. I'm using superfrost+ slides from fisher. Does anyone have any advice? Please help. From ritta69 <@t> iwon.com Fri Nov 20 12:19:54 2009 From: ritta69 <@t> iwon.com (ritta69.iwon) Date: Fri Nov 20 12:19:58 2009 Subject: [Histonet] frozen sections not adhering to slide Message-ID: <20091120131954.2772@web008.roc2.bluetie.com> I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose until the brains sink and flash freeze in isopentane. All seems like pretty standard stuff. After cutting 45 um thick sections and storing them in antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene Glycol), I mount them on slides. After letting them dry overnight, I rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to wrinkle up and start coming of the slide during this first step. I'm using superfrost+ slides from fisher. Does anyone have any advice? Please help. ------------------------------------------------------------ Weight Loss Program Best Weight Loss Program - Click Here! http://tagline.iwon.com/c?cp=DNzWnQGT6RwmX-EIosC0AQAAKZwW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqW_0= From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 20 12:24:23 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Nov 20 12:24:37 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892240@EXMBMCB15.ucsfmedicalcenter.org> The disclaimer is only for ASR antibodies. They don't have to be labeled "experimental" because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO antibodies under CLIA regs as long as you do the full documented validation. Interestingly there is no suggested disclaimer for RUO antibodies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 9:55 AM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Nov 20 12:26:19 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Nov 20 12:26:38 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF7892240@EXMBMCB15.ucsfmedicalcenter.org> References: <1AAF670737F193429070841C6B2ADD4CF7892240@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: Just can't bill for RUO, correct? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 13:24 To: Foshey, Annette; (histonet@lists.utsouthwestern.edu) Subject: RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports The disclaimer is only for ASR antibodies. They don't have to be labeled "experimental" because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO antibodies under CLIA regs as long as you do the full documented validation. Interestingly there is no suggested disclaimer for RUO antibodies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 9:55 AM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cevanish <@t> mont-hosp.com Fri Nov 20 12:28:15 2009 From: cevanish <@t> mont-hosp.com (Chris Evanish) Date: Fri Nov 20 12:28:48 2009 Subject: [Histonet] zinc or alcoholic formalin In-Reply-To: <20091120180532824@smtp442.redcondor.net> References: <20091120180532824@smtp442.redcondor.net> Message-ID: <4B06996C.6034.00E2.0@mont-hosp.com> Does anyone use zinc formalin or alcoholic formalin on their tissue processors? If so what is your routine run program Chris Montgomery Hospital >>> 11/20/2009 1:05 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Second Call for Manuscripts for Special Issue of Journal of Histotechnology (gayle callis) 2. Preventing slide labeling mistakes (Morken, Tim) 3. Re: Preventing slide labeling mistakes (Lynette Pavelich) 4. Re: Fwd: foxp3 ab ihc (Marilyn Tyler) 5. RE: Preventing slide labeling mistakes (Mike Pence) 6. RE: SPAM-LOW: [Histonet] Re: freezing skeletal muscle (Patsy Ruegg) 7. Re: Preventing slide labeling mistakes (Victor Tobias) 8. RE: Preventing slide labeling mistakes (Morken, Tim) 9. RE: Preventing slide labeling mistakes (Sebree Linda A) 10. RE: Preventing slide labeling mistakes (Armandi, Arlene) 11. CAP guidelines for positive controls (Tiana Fountain) 12. CAP QUESTION ANP.12425 ASR disclaimer on pathology reports (Foshey, Annette) 13. course recommendation (Mike Tighe) 14. Re: CAP QUESTION ANP.12425 ASR disclaimer on pathology reports (Rene J Buesa) 15. AW: [Histonet] Preventing slide labeling mistakes (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Thu, 19 Nov 2009 16:11:37 -0700 From: "gayle callis" Subject: [Histonet] Second Call for Manuscripts for Special Issue of Journal of Histotechnology To: "Histonet" Message-ID: <002301ca696d$a65f0fc0$f31d2f40$@callis@bresnan.net> Content-Type: text/plain; charset="utf-8" Dear Histonet Colleagues, A second call for manuscripts for Special Issue of Journal of Histotechnology: The Journal of Histotechnology (JOH) editors, Editorial Board, and guest editor Dr. Richard Cartun are pleased to announce a call for manuscripts for the March 2010 special issue of JOH, with focus on Immunohistochemistry and In Situ Hybridization. Technical notes and methodology-focused contributions are particularly encouraged; clinical, research, and veterinary topics are welcome. Manuscripts must be submitted prior to November 30, 2009 in order to be considered for the March issue. Manuscripts may be sent to Ms. Jane Jacobi, Managing Editor, at joh@nsh.org. Authors wishing pre-submission guidance with the writing process and assignment of a writing partner may contact Ms. Gayle Callis, JOH Assistant Editor, at gayle.callis@bresnan.net. Information for authors is detailed at www.nsh.org under Journal of Histotechnology. and an example of a Technical Note format is available upon request from Ms. Callis and NSH office. Gayle M. Callis JOH Assistant Editor National Society for Histotechnology Journal of Histotechnology 10320 Little Patuxent Pkwy #804 Columbia, MD 21044 P: 443-535-4060 E: joh@nsh.org ------------------------------ Message: 2 Date: Thu, 19 Nov 2009 16:20:16 -0800 From: "Morken, Tim" Subject: [Histonet] Preventing slide labeling mistakes To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892093@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ------------------------------ Message: 3 Date: Thu, 19 Nov 2009 22:52:07 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Preventing slide labeling mistakes To: , Message-ID: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 20 Nov 2009 07:52:05 +0200 From: "Marilyn Tyler" Subject: Re: [Histonet] Fwd: foxp3 ab ihc To: "histonet" Message-ID: <4B064AA5020000900008096B@gwiasmtp.uct.ac.za> Content-Type: text/plain; charset=US-ASCII Thanks to all in Histonet world posted on Heathers behalf who is still having problems with her access Marilyn >>> Heather McCleod 2009/11/19 10:43 AM >>> Sorry to bother you Marilyn. Please forward. I have an answer, i.e. confirmation of what I would like to use. Thanks to all who replied to my foxp3 query. Heather >>> "Marilyn Tyler" 2009/11/18 09:47 AM >>> Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 20 Nov 2009 08:37:53 -0600 From: "Mike Pence" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Lynette Pavelich" , , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CA2@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 20 Nov 2009 07:52:54 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: freezing skeletal muscle To: "'Robert Richmond'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Especially for bone, I have found that formalin fixation and then infiltrating in 30% sucrose before freezing the way Samuri described works the very best for me. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, November 19, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: freezing skeletal muscle Galina Deyneko asks about freezing skeletal muscle. I'm not sure that temperature control of the isopentane is too important - it should be very slightly viscous. But do get rid of explosive isopentane and get a non-flammable substitute - see my previous posts on this topic. The technique for freezing skeletal muscle is easier demonstrated than described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat it with talc powder so that it appears white on the surface. Then dip the specimen in and out of the isopentane - roughly two dips per second - until it is frozen solid. That will eliminate the ice crystal artifact. But you have to try it a few times before it will quite work for you. Set aside one dead rat and one afternoon. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 20 Nov 2009 07:10:36 -0800 From: Victor Tobias Subject: Re: [Histonet] Preventing slide labeling mistakes To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu, Timothy.Morken@ucsfmedctr.org Message-ID: <4B06B16C.2000507@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Lynette, You might want to also investigate different fonts if that is an option. Keeping the same size but using a different font can make a world of difference. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Lynette Pavelich wrote: > Hi Tim, > We do everything you do, but we also match our blocks to the written > number on the slides. We catch the majority of our mistakes at that > point. The other is because the font on the label is so small, we have > a hard time seeing the difference between 5's & 6's!!! I'm sure it's > not our ages or anything! We're working with CoPath to get a larger > font!! Ahhh, getting old is SO fun! And yes, reading glasses work > pretty good!! LOL > > Have a great weekend everyone!! > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > MSH Competency Coordinator > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > email: Lpaveli1@hurleymc.com > ph: 810-257-9948 > fax: 810-762-7082 > >>>> "Morken, Tim" 11/19/09 7:20 PM >>> >>>> > Hi, Can people share their procedures for preventing manual slide > labeling mistakes? No need to include barcoding - we are exploring that > but it is a ways off. > > We currently have a manual process: > We prohibit pre-labeling slides in batches (many blocks/slides at one > time), require labeling slides (hand-written) only at the time of > cutting a single block, and matching paper label to the slide after > staining. We don't currently match blocks to slides. > > Thanks for any info! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 20 Nov 2009 08:41:47 -0800 From: "Morken, Tim" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Mike Pence" , "Lynette Pavelich" , "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 20 Nov 2009 10:55:04 -0600 From: "Sebree Linda A" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Morken, Tim" , "Mike Pence" , "Lynette Pavelich" , Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF677@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" If the matching is done at the time of sectioning, i.e. making sure the block you are about to cut matches the slide you have ready to pick up that block's section(s), it will reduce mislabeling errors....and its GLP! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 10:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 20 Nov 2009 09:03:08 -0800 From: "Armandi, Arlene" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Morken, Tim" , "Mike Pence" , "Lynette Pavelich" , Message-ID: Content-Type: text/plain; charset="US-ASCII" One more suggestion. Have you considered using Xylene resistant slide labels? We print the labels at the time we put the blocks in order. We then put the corresponding labels on the trays with the blocks. When the tech picks up a tray, they have the labels ready to put directly onto the slides as they cut. This eliminates the labeling errors resulting from the matching the hand written slides (sometimes illegible) to the labels, after the slides have been stained. Just a thought. Arlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 8:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ------------------------------ Message: 11 Date: Fri, 20 Nov 2009 11:14:23 -0600 From: "Tiana Fountain" Subject: [Histonet] CAP guidelines for positive controls To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 12 Date: Fri, 20 Nov 2009 11:54:59 -0600 From: "Foshey, Annette" Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ------------------------------ Message: 13 Date: Fri, 20 Nov 2009 12:57:36 -0500 From: "Mike Tighe" Subject: [Histonet] course recommendation To: Message-ID: <4B069246.26E4.00EE.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike ------------------------------ Message: 14 Date: Fri, 20 Nov 2009 09:58:17 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: "\(histonet@lists.utsouthwestern.edu\)" , AnnetteFoshey Message-ID: <654236.83215.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We used to add that the methods were experimental. Ren? J. --- On Fri, 11/20/09, Foshey, Annette wrote: From: Foshey, Annette Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Date: Friday, November 20, 2009, 12:54 PM What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 20 Nov 2009 18:59:49 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Preventing slide labeling mistakes To: "'Morken, Tim'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We write a "macro-protocol" at the time of grossing with all blocknumbers and additional identifer. This paper is matched the first time with all blocks after embedding and before cutting. Missing blocks or wrong labelled blocks are found. The second time the paper is matched with all slides after staining and before delivering. Missing and wrong labelled slides are found. Additional the slide-number and material is matched with the "case sheet". The slides are labelled batchwise with 10 per batch immediatly before cutting. The identifiers (letters, "lateral", "resection-margin", etc.) are written on side of the cassette, so numbers can be written big enough. The numbers have maximum of 5 digits slash year. 12345/09 Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Morken, Tim Gesendet: Freitag, 20. November 2009 01:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Preventing slide labeling mistakes Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 22 **************************************** From milton.gomez <@t> aruplab.com Fri Nov 20 12:31:27 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Fri Nov 20 12:32:35 2009 Subject: [Histonet] NYState licensing examination Message-ID: <6995F66A2196514DB0C4CAA5A32EA2938E51E9@postoffice01.aruplab.net> Dear Histonetters, I am collecting any hand-outs, pamphlets, archives, books, CD's, anything you may have extra or may be willing to share that can help me prepare for the New York State Histologic Technician Examination. The examination may be similar to the HT and HTL exam; so anything related to these examinations will help. Thank you all in advance, Milton Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.gomez@aruplab.com Web: www.aruplab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From sandra.moeller78 <@t> googlemail.com Fri Nov 20 13:11:48 2009 From: sandra.moeller78 <@t> googlemail.com (Sandra Moeller) Date: Fri Nov 20 13:11:52 2009 Subject: [Histonet] ISH Message-ID: Dear Histonetters, I have a problem with my ISH and maybe one of you can help me. I used probes I *know* they are working. I put the mice embryos (E 10.5) in BM Purple for staining. Unfortunately they turned black in a short time. They were not stained in the inside. It looks like that the staining precipitated on the surface and no reaction did start in the inside. A few weeks ago I did an ISH to ( same tissue) and put the embryos in BM Purple over night in the fridge, because normally the staining should stop. It did not ! Does anybody know something about this problem? Thanks in advance, Sandra Moeller From DKBoyd <@t> chs.net Fri Nov 20 13:17:25 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Nov 20 13:16:07 2009 Subject: [Histonet] Lumpectomy question Message-ID: Good Friday Afternoon, Does anyone know if there is a modifier for 88307 for Lumpectomies when 20-30 blocks are submitted. We are currently charging 88307 but the pathologist thinks there should be a modifier. Thanks. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From gu.lang <@t> gmx.at Fri Nov 20 13:16:49 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 20 13:16:56 2009 Subject: [Histonet] rhodanin stain fading Message-ID: <43D1E7D14F384014991D9301AF2D8733@dielangs.at> Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun From JWeems <@t> sjha.org Fri Nov 20 13:28:08 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Nov 20 13:29:00 2009 Subject: [Histonet] CMS/NCCI Update Dated October 1, 2009 Message-ID: According the one of our pathologist the above mentioned update clarified that CMS allows the billing of special stains performed on different blocks of the same Pathology specimen. Previously, it was understood that a special stain could only be billed once per specimen. Now it appears that a stain could be billed twice if performed on block 1 and block 3 of the same specimen. Has anyone else reached this conclusion? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From 41dmb41 <@t> gmail.com Fri Nov 20 13:47:17 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Fri Nov 20 13:47:40 2009 Subject: [Histonet] CMS/NCCI Update Dated October 1, 2009 In-Reply-To: References: Message-ID: Joyce, That's very interesting, as I remember going to great lengths a few years ago to be sure we had a "billing limiter" in place in CoPath to prevent the multiple charging of CPT codes on the same specimen. However, I looked up the document released on 10/1/09 and here's what I found in regards to the special and immuno CPT codes: 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. >From what all I can see, you're right; it seems that we can bill for multiple special stains on the same specimen, just not the same block (so tell Drs. Stargel and Sears it won't help them :) ). I'm going to present this to our pathologists and billing auditors for review. Thanks so much for sharing this with everyone!!! Drew On Fri, Nov 20, 2009 at 14:28, Weems, Joyce wrote: > According the one of our pathologist the above mentioned update > clarified that CMS allows the billing of special stains performed on > different blocks of the same Pathology specimen. ?Previously, it was > understood that a special stain could only be billed once per specimen. > Now it appears that a stain could be billed twice if performed on block > 1 and block 3 of the same specimen. Has anyone else reached this > conclusion? > > > > Thanks, > > Joyce > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. ?Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From svobach <@t> kmcpa.com Fri Nov 20 14:08:44 2009 From: svobach <@t> kmcpa.com (Stacy Vobach) Date: Fri Nov 20 14:08:52 2009 Subject: [Histonet] SEEKING PATHOLOGY LAB DIRECTOR Message-ID: <008401ca6a1d$43735c30$ca5a1490$@com> PATHOLOGY LAB DIRECTOR Kansas Medical Clinic in Topeka, Kansas is seeking a Pathology Laboratory Director who will be responsible for the planning, coordination and delivery of services within the lab. Responsibilities to include: . Coordinates and directs daily operations to ensure best business practices . Driving initiatives to improved efficiencies and quality results; ability to optimize work flow . Maintaining effective communication with staff, pathologists, and other healthcare providers . Acts as a liaison between the laboratory and other departments within KMC . Coaches and counsels employees in coordination with the clinic administrator . Remains informed of current trends in laboratory management Requirements: . 5 years clinical laboratory experience with at least 2 years in a managerial capacity . Knowledge of a laboratory information system (AP Easy) . Bachelor's Degree/ASCP Contact: Resume/Cover letter to: SVobach@kmcpa.com or call: (785) 354-1040 x329 Stacy L. Vobach Human Resource Manager Kansas Medical Clinic P.A. 2200 SW 6th Street Topeka, KS 66606 785-354-1040 x329 From Ken_Marissael <@t> vwr.com Fri Nov 20 14:13:32 2009 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Fri Nov 20 14:13:38 2009 Subject: [Histonet] Re: Preventing slide labeling mistakes In-Reply-To: <200911201737.nAKHbfDh025557@appliance01.vwr.com> Message-ID: Tim, Brady seems to have come up with an answer that looks like it could help you. This is designed for the small volume user that does not have the volume to warrant an automated instrument. This is still a manual system, but can eliminate mistakes. If you can check out this video on You Tube: http://www.youtube.com/watch?v=CrQFeT9Hydk Ken Marissael VWR Clinical Account Representative ken_marissael@vwr.com 516-448-5546 - cell 516-873-0655 - fax histonet-request@ lists.utsouthwest ern.edu To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject Histonet Digest, Vol 72, Issue 22 11/20/2009 01:05 PM Please respond to histonet@lists.ut southwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Second Call for Manuscripts for Special Issue of Journal of Histotechnology (gayle callis) 2. Preventing slide labeling mistakes (Morken, Tim) 3. Re: Preventing slide labeling mistakes (Lynette Pavelich) 4. Re: Fwd: foxp3 ab ihc (Marilyn Tyler) 5. RE: Preventing slide labeling mistakes (Mike Pence) 6. RE: SPAM-LOW: [Histonet] Re: freezing skeletal muscle (Patsy Ruegg) 7. Re: Preventing slide labeling mistakes (Victor Tobias) 8. RE: Preventing slide labeling mistakes (Morken, Tim) 9. RE: Preventing slide labeling mistakes (Sebree Linda A) 10. RE: Preventing slide labeling mistakes (Armandi, Arlene) 11. CAP guidelines for positive controls (Tiana Fountain) 12. CAP QUESTION ANP.12425 ASR disclaimer on pathology reports (Foshey, Annette) 13. course recommendation (Mike Tighe) 14. Re: CAP QUESTION ANP.12425 ASR disclaimer on pathology reports (Rene J Buesa) 15. AW: [Histonet] Preventing slide labeling mistakes (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Thu, 19 Nov 2009 16:11:37 -0700 From: "gayle callis" Subject: [Histonet] Second Call for Manuscripts for Special Issue of Journal of Histotechnology To: "Histonet" Message-ID: <002301ca696d$a65f0fc0$f31d2f40$@callis@bresnan.net> Content-Type: text/plain; charset="utf-8" Dear Histonet Colleagues, A second call for manuscripts for Special Issue of Journal of Histotechnology: The Journal of Histotechnology (JOH) editors, Editorial Board, and guest editor Dr. Richard Cartun are pleased to announce a call for manuscripts for the March 2010 special issue of JOH, with focus on Immunohistochemistry and In Situ Hybridization. Technical notes and methodology-focused contributions are particularly encouraged; clinical, research, and veterinary topics are welcome. Manuscripts must be submitted prior to November 30, 2009 in order to be considered for the March issue. Manuscripts may be sent to Ms. Jane Jacobi, Managing Editor, at < mailto:joh@nsh.org> joh@nsh.org. Authors wishing pre-submission guidance with the writing process and assignment of a writing partner may contact Ms. Gayle Callis, JOH Assistant Editor, at < mailto:gayle.callis@bresnan.net> gayle.callis@bresnan.net. Information for authors is detailed at www.nsh.org under Journal of Histotechnology. and an example of a Technical Note format is available upon request from Ms. Callis and NSH office. Gayle M. Callis JOH Assistant Editor National Society for Histotechnology Journal of Histotechnology 10320 Little Patuxent Pkwy #804 Columbia, MD 21044 P: 443-535-4060 E: joh@nsh.org ------------------------------ Message: 2 Date: Thu, 19 Nov 2009 16:20:16 -0800 From: "Morken, Tim" Subject: [Histonet] Preventing slide labeling mistakes To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892093@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ------------------------------ Message: 3 Date: Thu, 19 Nov 2009 22:52:07 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Preventing slide labeling mistakes To: , Message-ID: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 20 Nov 2009 07:52:05 +0200 From: "Marilyn Tyler" Subject: Re: [Histonet] Fwd: foxp3 ab ihc To: "histonet" Message-ID: <4B064AA5020000900008096B@gwiasmtp.uct.ac.za> Content-Type: text/plain; charset=US-ASCII Thanks to all in Histonet world posted on Heathers behalf who is still having problems with her access Marilyn >>> Heather McCleod 2009/11/19 10:43 AM >>> Sorry to bother you Marilyn. Please forward. I have an answer, i.e. confirmation of what I would like to use. Thanks to all who replied to my foxp3 query. Heather >>> "Marilyn Tyler" 2009/11/18 09:47 AM >>> Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 20 Nov 2009 08:37:53 -0600 From: "Mike Pence" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Lynette Pavelich" , , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CA2@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 20 Nov 2009 07:52:54 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: freezing skeletal muscle To: "'Robert Richmond'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Especially for bone, I have found that formalin fixation and then infiltrating in 30% sucrose before freezing the way Samuri described works the very best for me. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, November 19, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: freezing skeletal muscle Galina Deyneko asks about freezing skeletal muscle. I'm not sure that temperature control of the isopentane is too important - it should be very slightly viscous. But do get rid of explosive isopentane and get a non-flammable substitute - see my previous posts on this topic. The technique for freezing skeletal muscle is easier demonstrated than described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat it with talc powder so that it appears white on the surface. Then dip the specimen in and out of the isopentane - roughly two dips per second - until it is frozen solid. That will eliminate the ice crystal artifact. But you have to try it a few times before it will quite work for you. Set aside one dead rat and one afternoon. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 20 Nov 2009 07:10:36 -0800 From: Victor Tobias Subject: Re: [Histonet] Preventing slide labeling mistakes To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu, Timothy.Morken@ucsfmedctr.org Message-ID: <4B06B16C.2000507@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Lynette, You might want to also investigate different fonts if that is an option. Keeping the same size but using a different font can make a world of difference. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Lynette Pavelich wrote: > Hi Tim, > We do everything you do, but we also match our blocks to the written > number on the slides. We catch the majority of our mistakes at that > point. The other is because the font on the label is so small, we have > a hard time seeing the difference between 5's & 6's!!! I'm sure it's > not our ages or anything! We're working with CoPath to get a larger > font!! Ahhh, getting old is SO fun! And yes, reading glasses work > pretty good!! LOL > > Have a great weekend everyone!! > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > MSH Competency Coordinator > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > email: Lpaveli1@hurleymc.com > ph: 810-257-9948 > fax: 810-762-7082 > >>>> "Morken, Tim" 11/19/09 7:20 PM >>> >>>> > Hi, Can people share their procedures for preventing manual slide > labeling mistakes? No need to include barcoding - we are exploring that > but it is a ways off. > > We currently have a manual process: > We prohibit pre-labeling slides in batches (many blocks/slides at one > time), require labeling slides (hand-written) only at the time of > cutting a single block, and matching paper label to the slide after > staining. We don't currently match blocks to slides. > > Thanks for any info! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 20 Nov 2009 08:41:47 -0800 From: "Morken, Tim" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Mike Pence" , "Lynette Pavelich" , "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 20 Nov 2009 10:55:04 -0600 From: "Sebree Linda A" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Morken, Tim" , "Mike Pence" , "Lynette Pavelich" , Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF677@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" If the matching is done at the time of sectioning, i.e. making sure the block you are about to cut matches the slide you have ready to pick up that block's section(s), it will reduce mislabeling errors....and its GLP! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 10:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 20 Nov 2009 09:03:08 -0800 From: "Armandi, Arlene" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Morken, Tim" , "Mike Pence" , "Lynette Pavelich" , Message-ID: Content-Type: text/plain; charset="US-ASCII" One more suggestion. Have you considered using Xylene resistant slide labels? We print the labels at the time we put the blocks in order. We then put the corresponding labels on the trays with the blocks. When the tech picks up a tray, they have the labels ready to put directly onto the slides as they cut. This eliminates the labeling errors resulting from the matching the hand written slides (sometimes illegible) to the labels, after the slides have been stained. Just a thought. Arlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 8:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ------------------------------ Message: 11 Date: Fri, 20 Nov 2009 11:14:23 -0600 From: "Tiana Fountain" Subject: [Histonet] CAP guidelines for positive controls To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 12 Date: Fri, 20 Nov 2009 11:54:59 -0600 From: "Foshey, Annette" Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ------------------------------ Message: 13 Date: Fri, 20 Nov 2009 12:57:36 -0500 From: "Mike Tighe" Subject: [Histonet] course recommendation To: Message-ID: <4B069246.26E4.00EE.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike ------------------------------ Message: 14 Date: Fri, 20 Nov 2009 09:58:17 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: "\(histonet@lists.utsouthwestern.edu\)" , AnnetteFoshey Message-ID: <654236.83215.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We used to add that the methods were experimental. Ren? J. --- On Fri, 11/20/09, Foshey, Annette wrote: From: Foshey, Annette Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Date: Friday, November 20, 2009, 12:54 PM What is the current practice for meeting this regulation?? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580? ? ? ? Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 20 Nov 2009 18:59:49 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Preventing slide labeling mistakes To: "'Morken, Tim'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We write a "macro-protocol" at the time of grossing with all blocknumbers and additional identifer. This paper is matched the first time with all blocks after embedding and before cutting. Missing blocks or wrong labelled blocks are found. The second time the paper is matched with all slides after staining and before delivering. Missing and wrong labelled slides are found. Additional the slide-number and material is matched with the "case sheet". The slides are labelled batchwise with 10 per batch immediatly before cutting. The identifiers (letters, "lateral", "resection-margin", etc.) are written on side of the cassette, so numbers can be written big enough. The numbers have maximum of 5 digits slash year. 12345/09 Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Morken, Tim Gesendet: Freitag, 20. November 2009 01:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Preventing slide labeling mistakes Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 22 **************************************** From David.Johnson2fdd80 <@t> va.gov Fri Nov 20 14:32:58 2009 From: David.Johnson2fdd80 <@t> va.gov (Johnson, David B (Columbia)) Date: Fri Nov 20 14:33:03 2009 Subject: [Histonet] UNSUBSCRIBE Message-ID: UNSUBSCRIBE From NMargaryan <@t> childrensmemorial.org Fri Nov 20 14:37:41 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Nov 20 14:37:50 2009 Subject: [Histonet] anti-human Notch4 Message-ID: Hi friends, My PI is asking for Notch 4 IHC on FFPE tissue. Can any of you suggest me a good Ab (preferably mot mouse but anti-human Notch4) and protocol please! Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From dgaupp <@t> tulane.edu Fri Nov 20 14:38:02 2009 From: dgaupp <@t> tulane.edu (Gaupp, Dina D) Date: Fri Nov 20 14:38:06 2009 Subject: [Histonet] unsubscribe Message-ID: <447056A67472B241A330A525B4AF716701804409@EX02.ad.tulane.edu> unsubscribe Dina D. Gaupp, BS, MT Senior Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu From stamptrain <@t> yahoo.com Fri Nov 20 15:08:58 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Nov 20 15:09:02 2009 Subject: [Histonet] UNSUBSCRIBE In-Reply-To: References: Message-ID: <786608.4629.qm@web55808.mail.re3.yahoo.com> The link at the bottom of the email is where you go to unsubscribe. Roger Moretz, Ph.D. (ret) ----- Original Message ---- From: "Johnson, David B (Columbia)" To: Histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 3:32:58 PM Subject: [Histonet] UNSUBSCRIBE UNSUBSCRIBE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Fri Nov 20 15:09:59 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Nov 20 15:10:03 2009 Subject: [Histonet] unsubscribe In-Reply-To: <447056A67472B241A330A525B4AF716701804409@EX02.ad.tulane.edu> References: <447056A67472B241A330A525B4AF716701804409@EX02.ad.tulane.edu> Message-ID: <609183.92184.qm@web55807.mail.re3.yahoo.com> The link @ the bottom of the email is where you go to unsubscribe. Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: "Gaupp, Dina D" To: Histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 3:38:02 PM Subject: [Histonet] unsubscribe unsubscribe Dina D. Gaupp, BS, MT Senior Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beatrice.Debrosse-Serra <@t> pfizer.com Fri Nov 20 15:19:05 2009 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Fri Nov 20 15:19:14 2009 Subject: [Histonet] anti-human Notch4 In-Reply-To: References: Message-ID: I am interested in the same antibody. Thanks, Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, November 20, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] anti-human Notch4 Hi friends, My PI is asking for Notch 4 IHC on FFPE tissue. Can any of you suggest me a good Ab (preferably mot mouse but anti-human Notch4) and protocol please! Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Nov 20 15:57:03 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 20 15:57:31 2009 Subject: [Histonet] Open Position at Abbott Laboratories - Associate Scientist - Histology In-Reply-To: <447056A67472B241A330A525B4AF716701804409@EX02.ad.tulane.edu> References: <447056A67472B241A330A525B4AF716701804409@EX02.ad.tulane.edu> Message-ID: This great opportunity has been posted on www.Abbott.com. We have several good candidates, however, we are still accepting applications and interviewing for this position. Please follow the link below, or go to www.Abbott.com to the careers page. Feel free to contact me if I can answer any questions. Jackie O'Connor https://jobs.brassring.com/EN/ASP/TG/cim_jobdetail.asp?SID=^1UDCBiFidlPldoZKm29r8nLu72/Sf03jAAV9uOfsISuc59yzr5XRFGuB6R3dLkI4KWTImkQ5r6Yc_C_R__L_F_AvaV92N_slp_rhc_ktpWe1Nf020HWyKzUDZCfik=&jobId=683223&type=search&JobReqLang=1&recordstart=1&JobSiteId=50&JobSiteInfo=683223_50&GQId=0 From ttruscot <@t> vetmed.wsu.edu Fri Nov 20 16:09:03 2009 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Nov 20 16:09:08 2009 Subject: [Histonet] frozen sections not adhering to slide In-Reply-To: <20091120131928.653@web010.roc2.bluetie.com> References: <20091120131928.653@web010.roc2.bluetie.com> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4BBA1A0EF08@CVMMBX.vetmed.wsu.edu> Hi, I think I would try rinsing them (after the antifreeze) in a 95% alcohol/5% PBS X3 before the next step (mounting on slides?) Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ritta69.iwon Sent: Friday, November 20, 2009 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen sections not adhering to slide I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose until the brains sink and flash freeze in isopentane. All seems like pretty standard stuff. After cutting 45 um thick sections and storing them in antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene Glycol), I mount them on slides. After letting them dry overnight, I rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to wrinkle up and start coming of the slide during this first step. I'm using superfrost+ slides from fisher. Does anyone have any advice? Please help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Fri Nov 20 16:13:11 2009 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Nov 20 16:13:15 2009 Subject: [Histonet] frozen sections not adhering to slide In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB4BBA1A0EF08@CVMMBX.vetmed.wsu.edu> References: <20091120131928.653@web010.roc2.bluetie.com> <44F1D6D7EB8CC84F92859EE5C4E6ECB4BBA1A0EF08@CVMMBX.vetmed.wsu.edu> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4BBA1A0EF0A@CVMMBX.vetmed.wsu.edu> I don't know where that attachment came from on the previous reply,. Anybody know?, but I wouldn't open it -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, November 20, 2009 2:09 PM To: ritta69.iwon; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] frozen sections not adhering to slide Hi, I think I would try rinsing them (after the antifreeze) in a 95% alcohol/5% PBS X3 before the next step (mounting on slides?) Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ritta69.iwon Sent: Friday, November 20, 2009 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen sections not adhering to slide I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose until the brains sink and flash freeze in isopentane. All seems like pretty standard stuff. After cutting 45 um thick sections and storing them in antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene Glycol), I mount them on slides. After letting them dry overnight, I rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to wrinkle up and start coming of the slide during this first step. I'm using superfrost+ slides from fisher. Does anyone have any advice? Please help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From achertcoff <@t> anlis.gov.ar Fri Nov 20 16:54:42 2009 From: achertcoff <@t> anlis.gov.ar (Agustin Victor Chertcoff) Date: Fri Nov 20 16:54:46 2009 Subject: [Histonet] sahnet list server In-Reply-To: <23afd74d0911201451v1285bae6u10a08f4c79662d0d@mail.gmail.com> References: <23afd74d0911201451v1285bae6u10a08f4c79662d0d@mail.gmail.com> Message-ID: <23afd74d0911201454n5c7f14e9s85c9a134bc85065b@mail.gmail.com> ---------- Forwarded message ---------- From: Agustin Victor Chertcoff Date: 2009/11/20 Subject: sahnet list server To: histonet@lists.utsouthwestern.edu Hi Histonetters! The SAHNET is a listserver (in Spanish Language) for the Histology profession, that is managed as a service to the field of Histology by HT Agustin Victor Chertcoff & HT Norma Pozzo. SAHNET current has more than 250 members in the world, specially in South America, Argentine, Peru,Mexico, Costa Rica,Uruguay, Chile,Colombia, and Spain. you can subscribe here http://www.elistas.net/grupo/sahnet (say "suscribite") also visit www.ht.org.ar Argentine Society of Histotechnology SAH From galinadeyneko <@t> yahoo.com Fri Nov 20 17:05:11 2009 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Fri Nov 20 17:05:14 2009 Subject: [Histonet] Re: Freezing artifacts Message-ID: <279024.74947.qm@web33103.mail.mud.yahoo.com> Dear Colleagues. Thank you very much for all answers, they are really useful. Sorry , but one more question. Some time? during cryosectioning? and mounting on glass slide (from Fisher, gold series for frozen sections)? I get bubbles underneath the tissue and these spots look very bad. I tried to use cold slide, warm slide, RT slide -no difference. How i can eliminate this problem? Thank you in advance Galina Deyneko. Cambridge, MA From jnocito <@t> satx.rr.com Fri Nov 20 17:08:52 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Nov 20 17:08:58 2009 Subject: [Histonet] Lumpectomy question In-Reply-To: References: Message-ID: <295BB928871142DE9EBBB1F305C6FD38@JoePC> Debbie, as far as I know, there is no modifier, all you can charge is a 88307. That's the same case when you have a skin excision at a 88305. Even if it's a melanoma excision and it takes 20 blocks, you can only charge an 88305. The system is FAR from perfect. JTT ----- Original Message ----- From: To: Sent: Friday, November 20, 2009 1:17 PM Subject: [Histonet] Lumpectomy question > Good Friday Afternoon, > Does anyone know if there is a modifier for 88307 for Lumpectomies when > 20-30 blocks are submitted. > We are currently charging 88307 but the pathologist thinks there should be > a modifier. > Thanks. > > Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical > Center I > 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: > 804-765-5582 I dkboyd@chs.net > > > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Nov 20 17:13:25 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Nov 20 17:13:30 2009 Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF7892240@EXMBMCB15.ucsfmedicalcenter.org> References: <1AAF670737F193429070841C6B2ADD4CF7892240@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: I have a question similar to this one. If I take 2 IVD antibodies say cytokeratin AE1/AE3 and 34BF12 and make these into one cytokeratin cocktail, is this considered an ASR because I combined them into another antibody? Joe ----- Original Message ----- From: "Morken, Tim" To: "Foshey, Annette" ; Sent: Friday, November 20, 2009 12:24 PM Subject: RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports The disclaimer is only for ASR antibodies. They don't have to be labeled "experimental" because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO antibodies under CLIA regs as long as you do the full documented validation. Interestingly there is no suggested disclaimer for RUO antibodies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 9:55 AM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Fri Nov 20 17:14:51 2009 From: renafail <@t> bellsouth.net (Rena Fail) Date: Fri Nov 20 17:14:55 2009 Subject: [Histonet] rhodanin stain fading In-Reply-To: <43D1E7D14F384014991D9301AF2D8733@dielangs.at> References: <43D1E7D14F384014991D9301AF2D8733@dielangs.at> Message-ID: <2362.35817.qm@web180309.mail.gq1.yahoo.com> Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of ?the day. Make sure your xylene is fresh?and coverslip immediately after staining Rena Fail ----- Original Message ---- From: Gudrun Lang To: histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Nov 21 01:24:14 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Nov 21 01:24:21 2009 Subject: AW: [Histonet] rhodanin stain fading In-Reply-To: <2362.35817.qm@web180309.mail.gq1.yahoo.com> References: <43D1E7D14F384014991D9301AF2D8733@dielangs.at> <2362.35817.qm@web180309.mail.gq1.yahoo.com> Message-ID: <097A1151F8C147D2A86490ECFCB6B609@dielangs.at> Rena, we use butylacetat for clearing and before coverslipping with Pertex (xylolbased, resinous). Butylacetat tolerates ethanol-carryover more than xylene, but evapourates very fast after couverslipping. But is it possible, that butylacetat or Pertex itself cause the fading? Do you think coverslipping with a waterbased medium is a good alternative? Gudrun -----Urspr?ngliche Nachricht----- Von: Rena Fail [mailto:renafail@bellsouth.net] Gesendet: Samstag, 21. November 2009 00:15 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] rhodanin stain fading Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of ?the day. Make sure your xylene is fresh?and coverslip immediately after staining Rena Fail ----- Original Message ---- From: Gudrun Lang To: histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ael <@t> unc.edu Sat Nov 21 16:27:49 2009 From: ael <@t> unc.edu (Ann Lowder) Date: Sat Nov 21 16:27:53 2009 Subject: [Histonet] Re: Preventing slide labeling mistakes In-Reply-To: <200911210728.nAL7S6wj000799@smtpsrv1.isis.unc.edu> References: <200911210728.nAL7S6wj000799@smtpsrv1.isis.unc.edu> Message-ID: <20091121172749.e8yivgtuww44gkoo@webmail3.isis.unc.edu> Tim, Labeling mistakes are difficult to fix if you are already not batch-labeling. However, sometimes you can help by rearranging how you put your information on the slide. For example, we have surgical numbers like P09-12345, but if you break that number up into two lines like P09 on the top and 12345 below, there are less numbers to overlook. If you have block letters and numbers too like we do, try putting them on the opposite side of the surgical number. It is much easier to catch a mistake in a string of 3 or 4 numbers versus 6 or 7. Hope this helps. P09 A 1234 1 Ann Lowder, HT(ASCP) Manager of Pathology Presbyterian Hospital, NC From ael <@t> unc.edu Sat Nov 21 16:39:18 2009 From: ael <@t> unc.edu (Ann Lowder) Date: Sat Nov 21 16:39:22 2009 Subject: [Histonet] Two positions open in Charlotte, NC In-Reply-To: <200911210728.nAL7S6wj000799@smtpsrv1.isis.unc.edu> References: <200911210728.nAL7S6wj000799@smtpsrv1.isis.unc.edu> Message-ID: <20091121173918.ff6v9miqjk0848ww@webmail3.isis.unc.edu> There are 2 histotech positions available for our hospital currently in Charlotte, NC. We are looking for certified HT or HTLs for a 3rd and a 1st shift position. We have great employees and great pathologists! If interested, please email your resume to me and complete an application online at www.presbyterian.org. I look forward to hearing from those truly interested! Thank you, Ann Lowder, HT(ASCP) aelowder@novanthealth.org Manager of Pathology Presbyterian Hospital From adeade64 <@t> yahoo.com Fri Nov 20 19:36:31 2009 From: adeade64 <@t> yahoo.com (Ade Ade) Date: Sat Nov 21 20:22:22 2009 Subject: [Histonet] HISTOLOGY JOB IN CANADA Message-ID: <784992.3124.qm@web113919.mail.gq1.yahoo.com> ? ??? Hi, ?????? I am an histotechnologist currently practicing in the United States. I am looking for infos on how to get? job as either Histotechnologist or IHC-Tech in Canada. ?? I will be very grateful, if you guys could furnish me with all the necessary infos. ? Thanking you all for your usual cooperation. ? ? Ade Ade. From jkiernan <@t> uwo.ca Sun Nov 22 00:33:21 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Nov 22 00:33:26 2009 Subject: [Histonet] rhodanin stain fading Message-ID: Rena is right. Published methods prescribe aqueous mounting media. This probably is the most sensitive histochemical staining method for detecting copper. See Irons et al 1977 Arch. Path. Lab. Med. 101:298-301. The histochemical reagent for copper is not rhodanine (which exists but would not stain copper deposits) or rhodanin (which does not exist). It is paradimethylaminobenzylidenerhodanine. It's indexed as a D in the catalogues of vendors of chemicals. There is no short name for this reagent and technique. This is not a pedantic gripe. You need to have the right compound and also the right instructions for the technique. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Rena Fail Date: Friday, November 20, 2009 18:15 Subject: Re: [Histonet] rhodanin stain fading To: gu.lang@gmx.at, histonet@lists.utsouthwestern.edu > Alcohol will cause the stain to fade, even the small amt. that > is carried over to the xylene over the course of the day. Make > sure your xylene is fresh and coverslip immediately after staining > Rena Fail > > > ----- Original Message ---- > From: Gudrun Lang > To: histonet@lists.utsouthwestern.edu > Sent: Fri, November 20, 2009 2:16:49 PM > Subject: [Histonet] rhodanin stain fading > > Hi! > > Yesterday I did a rhodine stain for copper. Immediatly after > staining I saw > not many but distinct red granula in hepatocytes. > > I am new to this stain, so I was happy (and a little bit proud), > that it had > worked. > > I also stained one slide over night for comparison. Today > morning the first > stained slides has faded and not the smallest bit of red colour > could be > seen. > > > > Is this a common problem? What causes the fast fading? > > > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Nov 22 02:46:47 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Nov 22 02:46:56 2009 Subject: AW: [Histonet] rhodanin stain fading In-Reply-To: References: Message-ID: <68B2FEFCE66145DE8E762688BC7E85AD@dielangs.at> Thank you for answering, I use the right compound, but as a lazy girl I wrote the short name. But you are absolutely right, precision should also be important with the terminology in histotechnic. Next time I will try the aqueous mounting media. I took the procedure from your book, but did the mistake to exchange the mounting media. Gudrun Lang _____ Von: John Kiernan [mailto:jkiernan@uwo.ca] Gesendet: Sonntag, 22. November 2009 07:33 An: Rena Fail Cc: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] rhodanin stain fading Rena is right. Published methods prescribe aqueous mounting media. This probably is the most sensitive histochemical staining method for detecting copper. See Irons et al 1977 Arch. Path. Lab. Med. 101:298-301. The histochemical reagent for copper is not rhodanine (which exists but would not stain copper deposits) or rhodanin (which does not exist). It is paradimethylaminobenzylidenerhodanine. It's indexed as a D in the catalogues of vendors of chemicals. There is no short name for this reagent and technique. This is not a pedantic gripe. You need to have the right compound and also the right instructions for the technique. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Rena Fail Date: Friday, November 20, 2009 18:15 Subject: Re: [Histonet] rhodanin stain fading To: gu.lang@gmx.at, histonet@lists.utsouthwestern.edu > Alcohol will cause the stain to fade, even the small amt. that > is carried over to the xylene over the course of the day. Make > sure your xylene is fresh and coverslip immediately after staining > Rena Fail > > > ----- Original Message ---- > From: Gudrun Lang > To: histonet@lists.utsouthwestern.edu > Sent: Fri, November 20, 2009 2:16:49 PM > Subject: [Histonet] rhodanin stain fading > > Hi! > > Yesterday I did a rhodine stain for copper. Immediatly after > staining I saw > not many but distinct red granula in hepatocytes. > > I am new to this stain, so I was happy (and a little bit proud), > that it had > worked. > > I also stained one slide over night for comparison. Today > morning the first > stained slides has faded and not the smallest bit of red colour > could be > seen. > > > > Is this a common problem? What causes the fast fading? > > > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpowers <@t> dpspa.com Sun Nov 22 12:54:28 2009 From: mpowers <@t> dpspa.com (Marian Powers) Date: Sun Nov 22 12:54:32 2009 Subject: [Histonet] HT Opening Message-ID: <5d7de0e60911221054i5971a474y64a461eeb5aa8cfc@mail.gmail.com> DPS in Dover, Delaware, is currently seeking a full time histotech, day or evening shift. All inquiries please email mpower@dpspa.com -- Marian L. Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-0000 From richard.pattison <@t> gmail.com Mon Nov 23 08:01:39 2009 From: richard.pattison <@t> gmail.com (Richard Pattison) Date: Mon Nov 23 08:01:48 2009 Subject: [Histonet] cryojane tap transfer system Message-ID: <4B0A95C3.4090703@googlemail.com> Hi Everybody, I was hoping to get some advice - I'm cryosectioning plant tissues and transferring sections to slides using the Cryojane system. However, i'm having problems in transferring the sections without them falling apart during the tape transfer. I'm fixing my tissue for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then sectioning at between 2 and 14 microns. The sections seem to be ok but whenever i remove the adhesive tape from the slide a large part of the tissue is removed with it. As a result I lose the majority of my section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides from instrumedics) but neither have given satisfactory results. Does anyone have any suggestions as to how i could reduce the loss of tissue? Any advice would be much appreciated. Thanks Richard From leiker <@t> buffalo.edu Mon Nov 23 08:26:51 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Nov 23 08:26:55 2009 Subject: [Histonet] cryojane tap transfer system In-Reply-To: <4B0A95C3.4090703@googlemail.com> References: <4B0A95C3.4090703@googlemail.com> Message-ID: <5D6D90DC816B8E32D70F0BDD@CDYwxp1931.ad.med.buffalo.edu> Unless I'm missing something, I don't understand why people use this tape? It seems like a marketing gimmick to me...ol' fashion' melting of sections onto slides works perfectly for us... ? Regards, Merced --On Monday, November 23, 2009 9:01 AM -0500 Richard Pattison wrote: > Hi Everybody, > I was hoping to get some advice - I'm cryosectioning plant tissues and > transferring sections to slides using the Cryojane system. However, i'm > having problems in transferring the sections without them falling apart > during the tape transfer. I'm fixing my tissue for 24 hours in > ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then > sectioning at between 2 and 14 microns. The sections seem to be ok but > whenever i remove the adhesive tape from the slide a large part of the > tissue is removed with it. As a result I lose the majority of my section. > I've tried using both 1x and 1/2x slides (CFSA adhesive slides from > instrumedics) but neither have given satisfactory results. > Does anyone have any suggestions as to how i could reduce the loss of > tissue? Any advice would be much appreciated. > Thanks > Richard > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Beth.Fye <@t> HCAhealthcare.com Mon Nov 23 08:38:05 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Mon Nov 23 08:38:11 2009 Subject: [Histonet] Suggestions for Cassette Labeling Message-ID: <938F8EC5A524D34EB5796E23E52781D329A0FCA112@NADCWPMSGCMS05.hca.corpad.net> There have been some great suggestions for reducing or catching slide labeling errors. I'm interested in the same for cassette labeling. Currently, we have the patient's last name on one side of the cassette, and the site listed on the other. This makes it easier to identify blocks if they are numbered incorrectly. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From trathborne <@t> somerset-healthcare.com Mon Nov 23 08:41:14 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Nov 23 08:41:19 2009 Subject: [Histonet] Suggestions for Cassette Labeling In-Reply-To: <938F8EC5A524D34EB5796E23E52781D329A0FCA112@NADCWPMSGCMS05.hca.corpad.net> Message-ID: That seems like a lot of effort. Do your pathologists do the grossing? or PA's? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fye Beth Sent: Monday, November 23, 2009 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Suggestions for Cassette Labeling There have been some great suggestions for reducing or catching slide labeling errors. I'm interested in the same for cassette labeling. Currently, we have the patient's last name on one side of the cassette, and the site listed on the other. This makes it easier to identify blocks if they are numbered incorrectly. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From LSebree <@t> uwhealth.org Mon Nov 23 09:35:07 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Nov 23 09:35:11 2009 Subject: [Histonet] Reference labs doing parafibromin? Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF67E@UWHC-MAIL01.uwhis.hosp.wisc.edu> Good Monday morning, One of our pathologists is getting pressure from the endocrinologists to bring on Parafibromin (gene: hrpt2). Are there any reference labs out there offering this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From kelleydurden <@t> pathology.ufl.edu Mon Nov 23 09:44:21 2009 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Mon Nov 23 09:45:24 2009 Subject: [Histonet] Mouse eyes Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE0956C7AD@HSC-CMS01.ad.ufl.edu> My question concerns mouse eyes. Can anyone send me their fixation suggestions? We are receiving mouse eyes that end up with a "wavy" look instead of a nicely defined "C" and have no explanation for this occurrence. Can anyone send me their mouse eye processing schedule? We have a protocol that has proved fast and true but would welcome other suggestions. Can you give me a good idea for making sure sections stick to slides well? Gold Plus? What else? Has anyone experienced a retinal detachment after or upon staining? Retina attached after sectioning - detached after staining - just routine H&E. Should I heat for an extended period of time? Kelley Durden, HT ASCP University of Florida Molecular Pathology Core From Susan.Walzer <@t> HCAHealthcare.com Mon Nov 23 09:48:19 2009 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Nov 23 09:48:26 2009 Subject: [Histonet] Job Opening Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AEAB5E4B0@FWDCWPMSGCMS09.hca.corpad.net> We have an opening for a tech, days no weekends at St Pete.General Hospital in St Pete,FL. Call our HR dept at 727 384-1414 X 4814. From gayle.callis <@t> bresnan.net Mon Nov 23 11:20:58 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Nov 23 11:21:35 2009 Subject: Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system Message-ID: <000101ca6c61$541e4d60$fc5ae820$@callis@bresnan.net> You wrote: Unless I'm missing something, I don't understand why people use this tape? It seems like a marketing gimmick to me...ol' fashion' melting of sections onto slides works perfectly for us... ? Regards, Merced Merced, Yes, you are missing something. If you have ever tried to cryosection undecalcified bone or extremely difficult tissues that simply will not result in "ol' fashion' melting" onto a slide , then you would understand why people use this unique cryosectioning system. It is not some "marketing gimmick" but an unique instrument helping many laboratories obtain frozen sections that otherwise are scrunched up, shattered, and destroyed. I suggest you go to the Instrumedics website or www.alphelys.com for a superb slide show and learn how this instrument works before making assumptions about a technology that serves many of us more than well. A happy, informed user of the Cryojane................ Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT As for what Richard wrote: Hi Everybody, I was hoping to get some advice - I'm cryosectioning plant tissues and transferring sections to slides using the Cryojane system. However, i'm having problems in transferring the sections without them falling apart during the tape transfer. I'm fixing my tissue for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then sectioning at between 2 and 14 microns. The sections seem to be ok but whenever i remove the adhesive tape from the slide a large part of the tissue is removed with it. As a result I lose the majority of my section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides from instrumedics) but neither have given satisfactory results. Does anyone have any suggestions as to how i could reduce the loss of tissue? Any advice would be much appreciated. Thanks Richard Dear Richard, I could be the fixative you are using that causes the problem. If the fixative contains alcohol, the alcohol acts as antifreeze when you try to snap freeze a tissue, animal or plant. The alcohol may cause problems with how the pink tape sticks to the face of the plant tissue, and allows them to fall apart during the tape transfer. If you rinse away the fixative, then you should cryoprotect the fixed plant tissue with 30% sucrose before snap freezing. vbThis will remove the alcohol. If cryoprotection causes problems with the final staining results, then try unfixed plant tissue, snap freeze, Cryojane tape transfer the section and then fix the transferred plant section in your favorite fixative. You may have to optimize the time in fixative though. Other suggestions: Do a double UV flash, but wait for 15 to 20 seconds between flashes. You must allow the UV light source (capacitor) build up enough charge to work properly. This double flash seems to help polymerize the coating more thoroughly, and the section should transfer better. Also, the tape must be removed at an angle across the slide, very slowly, and inside the cryostat (I am sure you probably do this already.) Also, try the 4X slide if you still have problems with 1/2X and 1X slides. You might ask Leica to send you a few to try before investing in a whole box of these. Contact Emmanuel Mineo, Intrumedics Product Manager emmanuel.mineo@leica-microsystems.com for the 4X slides. Manny is a nice gentleman who has worked with Cryojane for many years and has always been helpful to us. Once again, do the double UV light flash with the 4X slides. They are gooey, but may/should hold more securely. With undecalcified bone, we use the 1/2X but do the double flash routinely for all sections. Good luck Gayle Callis From christiegowan <@t> msn.com Mon Nov 23 11:22:40 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Mon Nov 23 11:22:44 2009 Subject: [Histonet] cryojane tap transfer system In-Reply-To: <5D6D90DC816B8E32D70F0BDD@CDYwxp1931.ad.med.buffalo.edu> References: <4B0A95C3.4090703@googlemail.com>, <5D6D90DC816B8E32D70F0BDD@CDYwxp1931.ad.med.buffalo.edu> Message-ID: Hi Richard, I am assuming that you are using the flash in the cryojane system to adhere the specimen to the slide. Make sure your tape is not too old and don't store it in the cryostat. It sounds like you are able to get the section but unable to get it to adhere. Make sure you pass your roller over the tape and slide several times before putting it under the flash and always use a positive charged slide from a freshly opened box. Good luck. I hope this helps a little Christie From collette2 <@t> mail.llnl.gov Mon Nov 23 12:13:24 2009 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Mon Nov 23 12:13:31 2009 Subject: [Histonet] cryojane tap transfer system In-Reply-To: <4B0A95C3.4090703@googlemail.com> References: <4B0A95C3.4090703@googlemail.com> Message-ID: Hello, Richard, I have done some sectioning of undecalcified bone with the Cryojane (hallelujah! it works! to paraphrase Ms. Callis) and one thing is that the sections won't cross-link if the tapes or the slides are being stored in the presence of UV light (then they are pre-crosslinked!), keep them dark and dry when not using, and don't take them into and out of the cryostat, only bring in what you need. I have also found that thicker sections don't crosslink as well, I've had trouble with sections over 6um (although I am far from am expert at cryosectioning). Temperature is also somewhat of a factor, I've found that I would section at colder temperature than I normally would for that tissue to get it to crosslink well- sounds counterintuitive, but there it is. This is purely based on my own trial and error, take it with a grain of salt and good luck. Sincerely, Nicole Collette LLNL/UC Berkeley At 9:01 AM -0500 11/23/09, Richard Pattison wrote: >Hi Everybody, >I was hoping to get some advice - I'm cryosectioning plant tissues >and transferring sections to slides using the Cryojane system. >However, i'm having problems in transferring the sections without >them falling apart during the tape transfer. I'm fixing my tissue >for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, >snap-freezing and then sectioning at between 2 and 14 microns. The >sections seem to be ok but whenever i remove the adhesive tape from >the slide a large part of the tissue is removed with it. As a result >I lose the majority of my section. I've tried using both 1x and 1/2x >slides (CFSA adhesive slides from instrumedics) but neither have >given satisfactory results. >Does anyone have any suggestions as to how i could reduce the loss >of tissue? Any advice would be much appreciated. >Thanks >Richard > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://*lists.utsouthwestern.edu/mailman/listinfo/histonet From donna_suresch <@t> merck.com Mon Nov 23 13:02:06 2009 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Mon Nov 23 13:02:14 2009 Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 26 In-Reply-To: References: Message-ID: RE: Cryojane Tape Transfer System I have found that you need to have your tape at the same temp as your tissue to have your tissue transfer completely from the tape. I set everything up in the morning putting the tape in the cryostat and get everything equilibrated first for about 2 hours or so. Hope it helps. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, November 23, 2009 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 72, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. HT Opening (Marian Powers) 2. cryojane tap transfer system (Richard Pattison) 3. Re: cryojane tap transfer system (Merced M Leiker) 4. Suggestions for Cassette Labeling (Fye Beth) 5. RE: Suggestions for Cassette Labeling (Rathborne, Toni) 6. Reference labs doing parafibromin? (Sebree Linda A) 7. Mouse eyes (Durden, Kelley) 8. Job Opening (Walzer Susan) 9. Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system (gayle callis) 10. RE: cryojane tap transfer system (CHRISTIE GOWAN) ---------------------------------------------------------------------- Message: 1 Date: Sun, 22 Nov 2009 13:54:28 -0500 From: Marian Powers Subject: [Histonet] HT Opening To: histonet@lists.utsouthwestern.edu Message-ID: <5d7de0e60911221054i5971a474y64a461eeb5aa8cfc@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 DPS in Dover, Delaware, is currently seeking a full time histotech, day or evening shift. All inquiries please email mpower@dpspa.com -- Marian L. Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-0000 ------------------------------ Message: 2 Date: Mon, 23 Nov 2009 09:01:39 -0500 From: Richard Pattison Subject: [Histonet] cryojane tap transfer system To: Histonet@lists.utsouthwestern.edu Message-ID: <4B0A95C3.4090703@googlemail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Everybody, I was hoping to get some advice - I'm cryosectioning plant tissues and transferring sections to slides using the Cryojane system. However, i'm having problems in transferring the sections without them falling apart during the tape transfer. I'm fixing my tissue for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then sectioning at between 2 and 14 microns. The sections seem to be ok but whenever i remove the adhesive tape from the slide a large part of the tissue is removed with it. As a result I lose the majority of my section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides from instrumedics) but neither have given satisfactory results. Does anyone have any suggestions as to how i could reduce the loss of tissue? Any advice would be much appreciated. Thanks Richard ------------------------------ Message: 3 Date: Mon, 23 Nov 2009 09:26:51 -0500 From: Merced M Leiker Subject: Re: [Histonet] cryojane tap transfer system To: Richard Pattison , Histonet@lists.utsouthwestern.edu Message-ID: <5D6D90DC816B8E32D70F0BDD@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Unless I'm missing something, I don't understand why people use this tape? It seems like a marketing gimmick to me...ol' fashion' melting of sections onto slides works perfectly for us... ? Regards, Merced --On Monday, November 23, 2009 9:01 AM -0500 Richard Pattison wrote: > Hi Everybody, > I was hoping to get some advice - I'm cryosectioning plant tissues and > transferring sections to slides using the Cryojane system. However, i'm > having problems in transferring the sections without them falling apart > during the tape transfer. I'm fixing my tissue for 24 hours in > ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then > sectioning at between 2 and 14 microns. The sections seem to be ok but > whenever i remove the adhesive tape from the slide a large part of the > tissue is removed with it. As a result I lose the majority of my section. > I've tried using both 1x and 1/2x slides (CFSA adhesive slides from > instrumedics) but neither have given satisfactory results. > Does anyone have any suggestions as to how i could reduce the loss of > tissue? Any advice would be much appreciated. > Thanks > Richard > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 4 Date: Mon, 23 Nov 2009 08:38:05 -0600 From: Fye Beth Subject: [Histonet] Suggestions for Cassette Labeling To: "histonet@lists.utsouthwestern.edu" Message-ID: <938F8EC5A524D34EB5796E23E52781D329A0FCA112@NADCWPMSGCMS05.hca.corpad.ne t> Content-Type: text/plain; charset="us-ascii" There have been some great suggestions for reducing or catching slide labeling errors. I'm interested in the same for cassette labeling. Currently, we have the patient's last name on one side of the cassette, and the site listed on the other. This makes it easier to identify blocks if they are numbered incorrectly. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 ------------------------------ Message: 5 Date: Mon, 23 Nov 2009 09:41:14 -0500 From: "Rathborne, Toni" Subject: RE: [Histonet] Suggestions for Cassette Labeling To: "Fye Beth" , Message-ID: Content-Type: text/plain; charset="utf-8" That seems like a lot of effort. Do your pathologists do the grossing? or PA's? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fye Beth Sent: Monday, November 23, 2009 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Suggestions for Cassette Labeling There have been some great suggestions for reducing or catching slide labeling errors. I'm interested in the same for cassette labeling. Currently, we have the patient's last name on one side of the cassette, and the site listed on the other. This makes it easier to identify blocks if they are numbered incorrectly. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr ------------------------------ Message: 6 Date: Mon, 23 Nov 2009 09:35:07 -0600 From: "Sebree Linda A" Subject: [Histonet] Reference labs doing parafibromin? To: "Histonet" Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF67E@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Good Monday morning, One of our pathologists is getting pressure from the endocrinologists to bring on Parafibromin (gene: hrpt2). Are there any reference labs out there offering this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 7 Date: Mon, 23 Nov 2009 10:44:21 -0500 From: "Durden, Kelley" Subject: [Histonet] Mouse eyes To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE0956C7AD@HSC-CMS01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" My question concerns mouse eyes. Can anyone send me their fixation suggestions? We are receiving mouse eyes that end up with a "wavy" look instead of a nicely defined "C" and have no explanation for this occurrence. Can anyone send me their mouse eye processing schedule? We have a protocol that has proved fast and true but would welcome other suggestions. Can you give me a good idea for making sure sections stick to slides well? Gold Plus? What else? Has anyone experienced a retinal detachment after or upon staining? Retina attached after sectioning - detached after staining - just routine H&E. Should I heat for an extended period of time? Kelley Durden, HT ASCP University of Florida Molecular Pathology Core ------------------------------ Message: 8 Date: Mon, 23 Nov 2009 09:48:19 -0600 From: Walzer Susan Subject: [Histonet] Job Opening To: "histonet@pathology.swmed.edu" Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AEAB5E4B0@FWDCWPMSGCMS09.hca.corpad.ne t> Content-Type: text/plain; charset="us-ascii" We have an opening for a tech, days no weekends at St Pete.General Hospital in St Pete,FL. Call our HR dept at 727 384-1414 X 4814. ------------------------------ Message: 9 Date: Mon, 23 Nov 2009 10:20:58 -0700 From: "gayle callis" Subject: Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system To: "'Histonet'" Cc: emmanuel.mineo@leica-microsystems.com Message-ID: <000101ca6c61$541e4d60$fc5ae820$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" You wrote: Unless I'm missing something, I don't understand why people use this tape? It seems like a marketing gimmick to me...ol' fashion' melting of sections onto slides works perfectly for us... ? Regards, Merced Merced, Yes, you are missing something. If you have ever tried to cryosection undecalcified bone or extremely difficult tissues that simply will not result in "ol' fashion' melting" onto a slide , then you would understand why people use this unique cryosectioning system. It is not some "marketing gimmick" but an unique instrument helping many laboratories obtain frozen sections that otherwise are scrunched up, shattered, and destroyed. I suggest you go to the Instrumedics website or www.alphelys.com for a superb slide show and learn how this instrument works before making assumptions about a technology that serves many of us more than well. A happy, informed user of the Cryojane................ Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT As for what Richard wrote: Hi Everybody, I was hoping to get some advice - I'm cryosectioning plant tissues and transferring sections to slides using the Cryojane system. However, i'm having problems in transferring the sections without them falling apart during the tape transfer. I'm fixing my tissue for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then sectioning at between 2 and 14 microns. The sections seem to be ok but whenever i remove the adhesive tape from the slide a large part of the tissue is removed with it. As a result I lose the majority of my section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides from instrumedics) but neither have given satisfactory results. Does anyone have any suggestions as to how i could reduce the loss of tissue? Any advice would be much appreciated. Thanks Richard Dear Richard, I could be the fixative you are using that causes the problem. If the fixative contains alcohol, the alcohol acts as antifreeze when you try to snap freeze a tissue, animal or plant. The alcohol may cause problems with how the pink tape sticks to the face of the plant tissue, and allows them to fall apart during the tape transfer. If you rinse away the fixative, then you should cryoprotect the fixed plant tissue with 30% sucrose before snap freezing. vbThis will remove the alcohol. If cryoprotection causes problems with the final staining results, then try unfixed plant tissue, snap freeze, Cryojane tape transfer the section and then fix the transferred plant section in your favorite fixative. You may have to optimize the time in fixative though. Other suggestions: Do a double UV flash, but wait for 15 to 20 seconds between flashes. You must allow the UV light source (capacitor) build up enough charge to work properly. This double flash seems to help polymerize the coating more thoroughly, and the section should transfer better. Also, the tape must be removed at an angle across the slide, very slowly, and inside the cryostat (I am sure you probably do this already.) Also, try the 4X slide if you still have problems with 1/2X and 1X slides. You might ask Leica to send you a few to try before investing in a whole box of these. Contact Emmanuel Mineo, Intrumedics Product Manager emmanuel.mineo@leica-microsystems.com for the 4X slides. Manny is a nice gentleman who has worked with Cryojane for many years and has always been helpful to us. Once again, do the double UV light flash with the 4X slides. They are gooey, but may/should hold more securely. With undecalcified bone, we use the 1/2X but do the double flash routinely for all sections. Good luck Gayle Callis ------------------------------ Message: 10 Date: Mon, 23 Nov 2009 17:22:40 +0000 From: CHRISTIE GOWAN Subject: RE: [Histonet] cryojane tap transfer system To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Richard, I am assuming that you are using the flash in the cryojane system to adhere the specimen to the slide. Make sure your tape is not too old and don't store it in the cryostat. It sounds like you are able to get the section but unable to get it to adhere. Make sure you pass your roller over the tape and slide several times before putting it under the flash and always use a positive charged slide from a freshly opened box. Good luck. I hope this helps a little Christie ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 26 **************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From NLinke <@t> mednet.ucla.edu Mon Nov 23 14:49:41 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Mon Nov 23 14:49:47 2009 Subject: [Histonet] job opening at UCLA Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE4968CD8A7@EMGMB1.ad.medctr.ucla.edu> Hi all, I have an opening for a Histotech III in the histology lab which (it was just posted). For this position, an HTL is required, no exceptions. We are looking for someone with 5+ years of experience in routine histology, manual specials, automated special stain experience is always a help, as well as someone with leadership skills. To apply, enter the job number H51527 in the 'Find Job Code box. https://jobs2.mednet.ucla.edu/css_external/CSSPage_BrowseJobs.ASP Salary range for this position is $36.83-$47.66 per hour DOE. If you are interested, please let me know! Thanks, Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From stamptrain <@t> yahoo.com Mon Nov 23 15:15:17 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Mon Nov 23 15:15:21 2009 Subject: [Histonet] Mouse eyes In-Reply-To: <92E6B93E0A3D544C87DDDE33E7608AAE0956C7AD@HSC-CMS01.ad.ufl.edu> References: <92E6B93E0A3D544C87DDDE33E7608AAE0956C7AD@HSC-CMS01.ad.ufl.edu> Message-ID: <986723.77043.qm@web55806.mail.re3.yahoo.com> Altho' retired for 2 years, we had a protocol that retained all parts of the eye quite well.? Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C for overnight.? Don't extend the fixation as this will cause the tissue to be too hard.? Wash for about 1hr in running tap water and process as usual for mouse tissue (if it works for mouse liver, it will work for the eyes).? Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: "Durden, Kelley" To: "histonet@lists.utsouthwestern.edu" Sent: Mon, November 23, 2009 10:44:21 AM Subject: [Histonet] Mouse eyes My question concerns mouse eyes. Can anyone send me their fixation suggestions?? We are receiving mouse eyes that end up with a "wavy" look instead of a nicely defined "C" and have no explanation for this occurrence. Can anyone send me their mouse eye processing schedule?? We have a protocol that has proved fast and true but would welcome other suggestions. Can you give me a good idea for making sure sections stick to slides well?? Gold Plus?? What else? Has anyone experienced a retinal detachment after or upon staining?? Retina attached after sectioning - detached after staining - just routine H&E.? Should I heat for an extended period of time? Kelley Durden, HT ASCP University of Florida Molecular Pathology Core _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon Nov 23 15:44:07 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Nov 23 15:44:25 2009 Subject: [Histonet] Mouse eyes In-Reply-To: <986723.77043.qm@web55806.mail.re3.yahoo.com> References: <92E6B93E0A3D544C87DDDE33E7608AAE0956C7AD@HSC-CMS01.ad.ufl.edu> <986723.77043.qm@web55806.mail.re3.yahoo.com> Message-ID: <001d01ca6c86$16b2bb30$44183190$@callis@bresnan.net> Davidsons fixative is popular for eyes. This has been discussed at great length on Histonet, so search the Archives. I believe it works for IHC. Gluteraldehyde can crosslink antigens too strongly if IHC is needed. Retina commonly detaches, at least with larger eyes we have worked with, when the eye section is flattened on a water bath. The water bath temperature used was a few degrees lower than normally used for other tissues. You should drain the slides well in upright position if using Plus charge. Lay flat on slide warmer, to dry at 37C to 40C overnight or longer as we do for decalcified bone sections. There are other ways to flatten eyes by floating an eye section on 5% alcohol water bath, then picking up on a slide, and slowly lowering the section (keep top part of paraffin of section attached to slide, and lowering this into a warm waterbath. The surface tension is reduced by the alcohol, allowing the eye to very slowly flatten, then pick up, drain well then dry flat. This takes a bit of practice but also works for decalcified bone when the softer cartilage likes to wrinkle versus the harder bone. Always pays to slow down speed of water rinsing, if the water flows too hard, during staining. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Moretz Sent: Monday, November 23, 2009 2:15 PM To: Durden, Kelley; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mouse eyes Altho' retired for 2 years, we had a protocol that retained all parts of the eye quite well.? Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C for overnight.? Don't extend the fixation as this will cause the tissue to be too hard.? Wash for about 1hr in running tap water and process as usual for mouse tissue (if it works for mouse liver, it will work for the eyes).? Roger Moretz, Ph.D. (ret.) ----- Original Message ---- From: "Durden, Kelley" To: "histonet@lists.utsouthwestern.edu" Sent: Mon, November 23, 2009 10:44:21 AM Subject: [Histonet] Mouse eyes My question concerns mouse eyes. Can anyone send me their fixation suggestions?? We are receiving mouse eyes that end up with a "wavy" look instead of a nicely defined "C" and have no explanation for this occurrence. Can anyone send me their mouse eye processing schedule?? We have a protocol that has proved fast and true but would welcome other suggestions. Can you give me a good idea for making sure sections stick to slides well?? Gold Plus?? What else? Has anyone experienced a retinal detachment after or upon staining?? Retina attached after sectioning - detached after staining - just routine H&E.? Should I heat for an extended period of time? Kelley Durden, HT ASCP University of Florida Molecular Pathology Core _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4630 (20091123) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4630 (20091123) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4630 (20091123) __________ The message was checked by ESET Smart Security. http://www.eset.com From Rcartun <@t> harthosp.org Mon Nov 23 16:24:53 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Nov 23 16:25:03 2009 Subject: [Histonet] TNF alpha Message-ID: <4B0AC565.7400.0077.1@harthosp.org> I have a colleague who is interested in doing IHC for TNF alpha on formalin-fixed, decalcified bone specimens. Is this possible? If so, I would appreciate hearing about your antibody and technique. Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From liz <@t> premierlab.com Mon Nov 23 16:28:06 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Nov 23 16:28:10 2009 Subject: [Histonet] TNF alpha In-Reply-To: <4B0AC565.7400.0077.1@harthosp.org> Message-ID: Richard it works. We purchase the antibody from Abcam. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, November 23, 2009 3:25 PM To: Histonet Subject: [Histonet] TNF alpha I have a colleague who is interested in doing IHC for TNF alpha on formalin-fixed, decalcified bone specimens. Is this possible? If so, I would appreciate hearing about your antibody and technique. Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Mon Nov 23 16:29:54 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Nov 23 16:29:59 2009 Subject: [Histonet] Open Position Message-ID: <274406.34318.qm@web113820.mail.gq1.yahoo.com> I have a full time position open for a histotech at NAMSA, located just south and east of Toledo in Northwood, Ohio.? This is a full time days position, and requires skills in embedding, sectioning, staining, etc.? Must be willing to work on animal tissues.? We work on many interesting specimens, and are always working to expand our capabilities. NAMSA is a CRO that performs a great deal of medical device testing.? Please feel free to look us up on the internet.? If you have any questions, you can respond to me at this email address, or at jsaby@namsa.com. Thanks! Joe Saby, BA HT(ASCP) From liz <@t> premierlab.com Mon Nov 23 16:32:55 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Nov 23 16:33:00 2009 Subject: [Histonet] TNF alpha In-Reply-To: <4B0AC565.7400.0077.1@harthosp.org> Message-ID: Sorry Guys I was thinking of IL-6 which I have done on bone and it works. We have worked with TNF-alpha (from Sigma) and our protocol does not requite retrieval I would think it would work on formic acid decalcified bone also, but I have not tried it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, November 23, 2009 3:25 PM To: Histonet Subject: [Histonet] TNF alpha I have a colleague who is interested in doing IHC for TNF alpha on formalin-fixed, decalcified bone specimens. Is this possible? If so, I would appreciate hearing about your antibody and technique. Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plott <@t> uab.edu Mon Nov 23 17:04:38 2009 From: plott <@t> uab.edu (Patricia F Lott) Date: Mon Nov 23 17:04:43 2009 Subject: [Histonet] need old processing baskets Message-ID: Help! I obtained an old Tissue-Tek II 4640 tissue processor, but I need the baskets, and a manual, if possible! Patty Lott, Laboratory Manager UAB CMBD Core Laboratory 205-934-2007 From kiran_g <@t> sbcglobal.net Mon Nov 23 17:54:59 2009 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Mon Nov 23 17:55:02 2009 Subject: [Histonet] 4 HISTOTECH POSITIONS IN kAISER In-Reply-To: <460802.66373.qm@web111603.mail.gq1.yahoo.com> Message-ID: <936080.94911.qm@web180101.mail.gq1.yahoo.com> Kaiser Regional Laboratory in Berkeley, CA has 4 full time Histotech openings, if interested please call Kiranjit @ 510-559-5404. ? --- On Mon, 11/16/09, Isaac O wrote: From: Isaac O Subject: [Histonet] NEW POSITION WANTED PLS To: histonet@lists.utsouthwestern.edu Date: Monday, November 16, 2009, 12:22 PM ? Hi, ?? I am looking for a new HISTOTECH/IHC position. I am? both HT(ASCP) and HTL(ASCP) certified. I am open to relocation most?especially , the MIDWEST, NORTH EAST, SOUTH EAST and the NORTH WEST. ?? I also have some management experience. ?Isaac. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Mon Nov 23 18:01:16 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Nov 23 18:02:21 2009 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 11/23/2009 and will not return until 11/30/2009. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From Fawn.Bomar <@t> HalifaxRegional.com Tue Nov 24 07:57:15 2009 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Nov 24 07:57:30 2009 Subject: [Histonet] Need a manual Message-ID: Hi everyone, I was wondering if anyone has a manual for the Tissue-Tek II Cryostat that they would be willing to share. Thank you Fawn Bomar, HT(ASCP) ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From Vickroy.Jim <@t> mhsil.com Tue Nov 24 09:19:19 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Nov 24 09:19:25 2009 Subject: [Histonet] TEMPERATURE RECORDING Message-ID: <24A4826E8EF0964D86BC5317306F58A54253E76914@mmc-mail.ad.mhsil.com> We have a master sheet for recording all temperatures, in which a tech daily fills in after checking all instruments and thermometers. Some of our instruments (such as refrigerators) also have a chart on them that we fill out. It seems redundant that we are logging temps on a chart and on a master list. What is everybody else doing? Do we just use charts on everything and forget the master log? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From 41dmb41 <@t> gmail.com Tue Nov 24 09:23:54 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Nov 24 09:24:18 2009 Subject: [Histonet] TEMPERATURE RECORDING In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54253E76914@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A54253E76914@mmc-mail.ad.mhsil.com> Message-ID: Here at our lab, we just record the temps on individual logs for each instrument. We don't have a master log. Drew On Tue, Nov 24, 2009 at 10:19, Vickroy, Jim wrote: > > We have a master sheet for recording all temperatures, in which a tech daily fills in after checking all instruments and thermometers. ? Some of our instruments (such as refrigerators) also have a chart on them that we fill out. ? It seems redundant that we are logging temps on a chart and on a master list. ? What is everybody else doing? ? Do we just use charts on everything and forget the master log? > > James Vickroy BS, HT(ASCP) > > Surgical ?and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Bauer.Karen <@t> mayo.edu Tue Nov 24 09:39:41 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Nov 24 09:39:47 2009 Subject: [Histonet] Mohs Histotech Needed Message-ID: <53FC421CC200C5429929EDE6C3676F306E1652@msgebe34> Hello all... We have the following position open: "Mohs Histotech needed for a new Mohs surgery practice with an established Mayo Health System facility. The position is full-time, split equally between Mohs and a traditional hospital lab. ASCP registry/eligibility required. Mohs experience preferred. Apply online at www.luthermidelfort.org or email lmcareers@mayo.edu with questions." As the info states, this position will be shared with the hospital Histology department. Applicants will need to be experienced in embedding, cutting, grossing, special stains, IP staining and have computer knowledge for data entry. You may also email or call me with questions as well. Thank you, Karen Karen L. Bauer HT/HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu From mpence <@t> grhs.net Tue Nov 24 09:47:03 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Nov 24 09:47:09 2009 Subject: [Histonet] TEMPERATURE RECORDING In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CA5@is-e2k3.grhs.net> Same here! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, November 24, 2009 9:24 AM To: Vickroy, Jim Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TEMPERATURE RECORDING Here at our lab, we just record the temps on individual logs for each instrument. We don't have a master log. Drew On Tue, Nov 24, 2009 at 10:19, Vickroy, Jim wrote: > > We have a master sheet for recording all temperatures, in which a tech > daily fills in after checking all instruments and thermometers. ? Some > of our instruments (such as refrigerators) also have a chart on them > that we fill out. ? It seems redundant that we are logging temps on a > chart and on a master list. ? What is everybody else doing? ? Do we > just use charts on everything and forget the master log? > > James Vickroy BS, HT(ASCP) > > Surgical ?and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Nov 24 10:07:13 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Nov 24 10:07:21 2009 Subject: [Histonet] RELIA Histology Job Alert and a few Holiday Shopping tips! Message-ID: Hi Histonetters! I hope you are having a wonderful Fall. The weather is getting cooler and the Holidays are right around the corner. I realize that at this time of year a job change is the furthest thing from most people?s minds but I have some great opportunities that I wanted to share along with some great tips for getting some good deals while holiday shopping. All of my positions are full time permanent positions with premier companies who offer excellent salaries, benefits and relocation assistance. Most of them are willing to look at onsite interviews and start dates after the holidays so if you or anyone you know might be looking now or after the first of the year it wouldn?t hurt to shoot me a quick e-mail at relia1@earthlink.net or give me a quick call toll free at 866-607-3542. Currently I have management positions in OR, WA, CA and NC. I also have tech positions in AZ, CA, FL, TX, MI, MA and NY. In addition I also have a PA position in NC. Holiday shopping is always fun, crazy, chaotic, inspiring, and challenging. Here are some tips that might save you some time, money and stress. Black Friday is the day after Thanksgiving the official first day of Holiday Shopping. If you want a heads up on the advertisements from your favorite stores go to the website: www.bfads.net They have a lot of the ads posted on their site NOW that will be in your newspaper on Thanksgiving morning. The Monday after Thanksgiving is known as Cyber Monday it is the busiest online shopping day of the year and there is another website that will give you heads up on deals for Cyber Monday. This website is www.cybermonday.com The best part of this site is that the stores that advertise on their site donate a percentage of sales to a scholarship fund. Again if you or anyone you know is interested in a new opportunity now or after the holidays get in touch with me. There are a lot of recruiters out there but remember I am the only one with the experience, connections and respect for you and your career that specializes exclusively in histology. I want to place you in a new position only if it is the right place, right time and right position for you. And I am always available for career advice, resume assistance or just a chat. Let me be your career advocate. Happy Holidays!!! Pam 866-607-3542 Relia1@earthlink.net Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From rjbuesa <@t> yahoo.com Tue Nov 24 10:28:28 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 24 10:28:32 2009 Subject: [Histonet] TEMPERATURE RECORDING In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3CA5@is-e2k3.grhs.net> Message-ID: <693940.2333.qm@web65701.mail.ac4.yahoo.com> I used to have a form that included all the instruments. One form per month and the temperature of all instruments were recorded?daily?simultaneously. Come the end of the month a new form was placed on one side of a VIP to start all over again. ? Ren? J.? --- On Tue, 11/24/09, Mike Pence wrote: From: Mike Pence Subject: RE: [Histonet] TEMPERATURE RECORDING To: "Drew Meyer" <41dmb41@gmail.com>, "Vickroy, Jim" Cc: Histonet@lists.utsouthwestern.edu Date: Tuesday, November 24, 2009, 10:47 AM Same here! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, November 24, 2009 9:24 AM To: Vickroy, Jim Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TEMPERATURE RECORDING Here at our lab, we just record the temps on individual logs for each instrument.? We don't have a master log. Drew On Tue, Nov 24, 2009 at 10:19, Vickroy, Jim wrote: > > We have a master sheet for recording all temperatures, in which a tech > daily fills in after checking all instruments and thermometers. ? Some > of our instruments (such as refrigerators) also have a chart on them > that we fill out. ? It seems redundant that we are logging temps on a > chart and on a master list. ? What is everybody else doing? ? Do we > just use charts on everything and forget the master log? > > James Vickroy BS, HT(ASCP) > > Surgical ?and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Nov 24 10:56:53 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Nov 24 10:59:13 2009 Subject: [Histonet] TEMPERATURE RECORDING In-Reply-To: <693940.2333.qm@web65701.mail.ac4.yahoo.com> References: <661949901A768E4F9CC16D8AF8F2838C017A3CA5@is-e2k3.grhs.net>, <693940.2333.qm@web65701.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D3B239DB0@LRGHEXVS1.practice.lrgh.org> We have one master form that covers the water baths, embedding units, paraffin pot, slide drying oven and refrigerator. Processors, Cryostat have their own maintenance/cleaning records. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, November 24, 2009 11:28 AM To: Drew Meyer; JimVickroy; Mike Pence Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TEMPERATURE RECORDING I used to have a form that included all the instruments. One form per month and the temperature of all instruments were recorded daily simultaneously. Come the end of the month a new form was placed on one side of a VIP to start all over again. Ren? J. --- On Tue, 11/24/09, Mike Pence wrote: From: Mike Pence Subject: RE: [Histonet] TEMPERATURE RECORDING To: "Drew Meyer" <41dmb41@gmail.com>, "Vickroy, Jim" Cc: Histonet@lists.utsouthwestern.edu Date: Tuesday, November 24, 2009, 10:47 AM Same here! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, November 24, 2009 9:24 AM To: Vickroy, Jim Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TEMPERATURE RECORDING Here at our lab, we just record the temps on individual logs for each instrument. We don't have a master log. Drew On Tue, Nov 24, 2009 at 10:19, Vickroy, Jim wrote: > > We have a master sheet for recording all temperatures, in which a tech > daily fills in after checking all instruments and thermometers. Some > of our instruments (such as refrigerators) also have a chart on them > that we fill out. It seems redundant that we are logging temps on a > chart and on a master list. What is everybody else doing? Do we > just use charts on everything and forget the master log? > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From leiker <@t> buffalo.edu Tue Nov 24 13:30:55 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Nov 24 13:31:12 2009 Subject: [Histonet] Purchasing a Cryostat Message-ID: <0EC310C077AD2F2857CF3129@CDYwxp1931.ad.med.buffalo.edu> Hi all, We're looking into purchasing our own cryostat (instead of paying to use someone else's all the time!). We are research, not clinical or industrial, and want a research-type model. Does anyone have any advice or experience with getting a new vs. a used cryostat? Or what brand you would recommend? (Shandon, Vibratome, etc.) Thanks so much! Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From DJenkins <@t> bwmc.umms.org Tue Nov 24 14:58:57 2009 From: DJenkins <@t> bwmc.umms.org (Jenkins, Dalena) Date: Tue Nov 24 14:59:05 2009 Subject: [Histonet] Proficiency test for CLO Message-ID: I am looking for information on whether there is a profiency test needed for resulting CLO tests (rapid urease test for Campylobacter). At our facility, the Endoscopist inserts the gastric biopsy into the cartridge at bedside and then sends to Histology for the Histology personnel to result after 24hrs. I have checked the College of American Pathologists website, but did not find a clear cut answer. I know the test is CLIA waived, but that doesn't necessarily mean that a proficiency test isn't required. Any information would be helpful. Thanks. Dalena Jenkins Histopathology Baltimore Washington Medical Center 410-787-4187 djenkins@bwmc.umms.org From leiker <@t> buffalo.edu Tue Nov 24 15:14:44 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Nov 24 15:14:50 2009 Subject: [Histonet] Purchasing a Cryostat In-Reply-To: <0EC310C077AD2F2857CF3129@CDYwxp1931.ad.med.buffalo.edu> References: <0EC310C077AD2F2857CF3129@CDYwxp1931.ad.med.buffalo.edu> Message-ID: Thanks so much for everyone who's replied so far (wow, a lot of responses, including from 2 reps)...I just talked with my boss... budget concerns may restrict us to a refurbished cryostat... Can anyone recommend a good refurbisher, and perhaps of Leica units? (the U.S. responses thus far were overwhelmingly in favor of Leica) Thanks again! Regards, Merced --On Tuesday, November 24, 2009 2:30 PM -0500 Merced M Leiker wrote: > Hi all, > > We're looking into purchasing our own cryostat (instead of paying to use > someone else's all the time!). We are research, not clinical or > industrial, and want a research-type model. > > Does anyone have any advice or experience with getting a new vs. a used > cryostat? Or what brand you would recommend? (Shandon, Vibratome, etc.) > Thanks so much! > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From rjbuesa <@t> yahoo.com Tue Nov 24 15:20:13 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 24 15:20:17 2009 Subject: [Histonet] Purchasing a Cryostat In-Reply-To: Message-ID: <786063.22083.qm@web65715.mail.ac4.yahoo.com> If you Google "cryostat" you will probably fing refurbished units. Ren? J. --- On Tue, 11/24/09, Merced M Leiker wrote: From: Merced M Leiker Subject: Re: [Histonet] Purchasing a Cryostat To: "Histonet" Date: Tuesday, November 24, 2009, 4:14 PM Thanks so much for everyone who's replied so far (wow, a lot of responses, including from 2 reps)...I just talked with my boss... budget concerns may restrict us to a refurbished cryostat... Can anyone recommend a good refurbisher, and perhaps of Leica units? (the U.S. responses thus far were overwhelmingly in favor of Leica) Thanks again! Regards, Merced --On Tuesday, November 24, 2009 2:30 PM -0500 Merced M Leiker wrote: > Hi all, > > We're looking into purchasing our own cryostat (instead of paying to use > someone else's all the time!). We are research, not clinical or > industrial, and want a research-type model. > > Does anyone have any advice or experience with getting a new vs. a used > cryostat? Or what brand you would recommend? (Shandon, Vibratome, etc.) > Thanks so much! > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214? USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Nov 24 15:26:16 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Nov 24 15:26:22 2009 Subject: [Histonet] Purchasing a Cryostat In-Reply-To: <786063.22083.qm@web65715.mail.ac4.yahoo.com> References: <786063.22083.qm@web65715.mail.ac4.yahoo.com> Message-ID: I should probably rephrase my query: Does anyone have experience or advice about a particular refurbisher as I'm a bit leary of just pulling one off the internet? Thank you. Regards, Merced --On Tuesday, November 24, 2009 1:20 PM -0800 Rene J Buesa wrote: > > If you Google "cryostat" you will probably fing refurbished units. > Ren? J. > > --- On Tue, 11/24/09, Merced M Leiker wrote: > > > From: Merced M Leiker > Subject: Re: [Histonet] Purchasing a Cryostat > To: "Histonet" > Date: Tuesday, November 24, 2009, 4:14 PM > > > Thanks so much for everyone who's replied so far (wow, a lot of > responses, > including from 2 reps)...I just talked with my boss... budget concerns > may > restrict us to a refurbished cryostat... > > Can anyone recommend a good refurbisher, and perhaps of Leica units? (the > U.S. responses thus far were overwhelmingly in favor of Leica) > > Thanks again! > > Regards, > Merced > > --On Tuesday, November 24, 2009 2:30 PM -0500 Merced M Leiker > wrote: > >> Hi all, >> >> We're looking into purchasing our own cryostat (instead of paying to use >> someone else's all the time!). We are research, not clinical or >> industrial, and want a research-type model. >> >> Does anyone have any advice or experience with getting a new vs. a used >> cryostat? Or what brand you would recommend? (Shandon, Vibratome, etc.) >> Thanks so much! >> >> Merced M Leiker >> Research Technician III >> Cardiovascular Medicine >> 348 Biomedical Research Building >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 USA >> leiker@buffalo.edu >> 716-829-6118 (Ph) >> 716-829-2665 (Fx) >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From NLinke <@t> mednet.ucla.edu Tue Nov 24 15:31:44 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Tue Nov 24 15:31:43 2009 Subject: [Histonet] Sakura Embedding Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE4968CDA33@EMGMB1.ad.medctr.ucla.edu> Is anyone using the Sakura TissueTek AutoTec automated embedding machine in their lab? Do you like it? Thank you! Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From Stephen.Clark1 <@t> hcahealthcare.com Tue Nov 24 15:59:22 2009 From: Stephen.Clark1 <@t> hcahealthcare.com (Clark Stephen - Myrtle Beach) Date: Tue Nov 24 15:59:34 2009 Subject: [Histonet] Report Question Message-ID: <213A638666ECDB4A8A81B5CF8646CC0B2A556C3F62@NADCWPMSGCMS05.hca.corpad.net> Hey all, My Pathologists want to start including pictures with their reports. The question is what systems do you recommend that are compatible with Meditech and what are the costs. Thanks, Steve Clark Histology Supervisor Grand Strand Regional Medical Center 843-692-1486 Lab 843-692-1459 Desk Stephen.Clark1@hcahealthcare.com [cid:image001.gif@01C9DADA.65BB9E10] From jstaruk <@t> masshistology.com Tue Nov 24 16:09:29 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue Nov 24 16:09:44 2009 Subject: [Histonet] In need of light bulbs In-Reply-To: Message-ID: Does anyone know what size bulb fits the Shandon embedding station, model #64000004? There are two of them at the spigot. Both of mine are burnt out and neither have any numbers on the glass or base! Gracias Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From laurie.colbert <@t> huntingtonhospital.com Tue Nov 24 17:15:19 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Nov 24 17:16:38 2009 Subject: [Histonet] Position in Pasadena, CA Message-ID: <57BE698966D5C54EAE8612E8941D76830767B7C0@EXCHANGE3.huntingtonhospital.com> Huntington Memorial Hospital has a full time Lab Processor / Lab Assistant position available immediately. The hours are 3:30 pm - midnight. The position is split between the grossing room and Cytology prep and can be very fast-paced at times. The salary range is $12.36 - $18.44/hr based on experience - Pathology experience preferred. Please submit an application at www.huntingtonhospital.com and click on "career opportunites." Laurie Colbert Huntington Hospital (626) 397-8620 From Kristopher.Kalleberg <@t> unilever.com Tue Nov 24 17:51:00 2009 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Nov 24 17:51:06 2009 Subject: [Histonet] Biopsy fixation Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0736E70D@NTRSEVS30002.s3.ms.unilever.com> Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is the maximum time we can leave the biopsies in 70% alcohol before processing into paraffin? Is there a better reagent, such as PBS, other than alcohol to leave the biopsy in for a few days before tissue processing. Thank you in advance. Kris From yujuan_dong <@t> yahoo.com Tue Nov 24 23:00:40 2009 From: yujuan_dong <@t> yahoo.com (Dong Yujuan) Date: Tue Nov 24 23:00:44 2009 Subject: [Histonet] difference between immunocytochemistry and immunohistochemistry-Fr Message-ID: <844307.59924.qm@web55501.mail.re4.yahoo.com> Hi all, I am using an monoclonal antibody to test a membrane protein (tested appilication include Flow cyt and western blot). It?was succsessfuly used in Immunocytochemistry (cell culture) before. However when I applied it in Immunohistochemistry (Frozen sections, human tissue), there was no signal at all (The fixation is the same). Is there so big difference between ICC and IHC-Fr? Is it my problem or the antibody's? Many thanks! ? Celia Chinese University of Hong Kong Hong Kong Yahoo!???????????????????! ??? http://hk.promo.yahoo.com/security/ ????! From annigyg <@t> gmail.com Wed Nov 25 02:47:31 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Nov 25 02:47:45 2009 Subject: [Histonet] Sakura Embedding In-Reply-To: <0C96F0BFE078D74C91A1C541D24A6AE4968CDA33@EMGMB1.ad.medctr.ucla.edu> References: <0C96F0BFE078D74C91A1C541D24A6AE4968CDA33@EMGMB1.ad.medctr.ucla.edu> Message-ID: i am - love it!! 2009/11/25 Linke, Noelle > Is anyone using the Sakura TissueTek AutoTec automated embedding machine in > their lab? Do you like it? > > Thank you! > Noelle > > No?lle Linke M.S., HTL(ASCP)QIHC > Manager, Histology Services > Department of Pathology & Laboratory Medicine > David Geffen School of Medicine at UCLA > Phone: 310-825-7397 > Pager: 97471 > nlinke@mednet.ucla.edu > > > > > > > ________________________________ > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may subject > you to federal and state penalties. If you are not the intended recipient, > please immediately notify us by return email, and delete this message from > your computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From b-frederick <@t> northwestern.edu Wed Nov 25 07:35:20 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Nov 25 07:35:39 2009 Subject: [Histonet] TNF alpha In-Reply-To: Message-ID: <4935529322AD42CD846716B7F4E32310@lurie.northwestern.edu> Same here- we use Abcam also. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Monday, November 23, 2009 4:28 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] TNF alpha Richard it works. We purchase the antibody from Abcam. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, November 23, 2009 3:25 PM To: Histonet Subject: [Histonet] TNF alpha I have a colleague who is interested in doing IHC for TNF alpha on formalin-fixed, decalcified bone specimens. Is this possible? If so, I would appreciate hearing about your antibody and technique. Thanks! Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Nov 25 08:33:22 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Nov 25 08:34:23 2009 Subject: [Histonet] Sakura Embedding In-Reply-To: References: <0C96F0BFE078D74C91A1C541D24A6AE4968CDA33@EMGMB1.ad.medctr.ucla.edu> Message-ID: <4B0D4032.5040604@pathology.washington.edu> As I recall you don't have to do anything special at the time of embedding, but what is it like for the person grossing? They have to correctly orientate the tissue or the specimen could be ruined? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Anne van Binsbergen wrote: > i am - love it!! > > 2009/11/25 Linke, Noelle > > >> Is anyone using the Sakura TissueTek AutoTec automated embedding machine in >> their lab? Do you like it? >> >> Thank you! >> Noelle >> >> No?lle Linke M.S., HTL(ASCP)QIHC >> Manager, Histology Services >> Department of Pathology & Laboratory Medicine >> David Geffen School of Medicine at UCLA >> Phone: 310-825-7397 >> Pager: 97471 >> nlinke@mednet.ucla.edu >> >> >> >> >> >> >> ________________________________ >> IMPORTANT WARNING: This email (and any attachments) is only intended for >> the use of the person or entity to which it is addressed, and may contain >> information that is privileged and confidential. You, the recipient, are >> obligated to maintain it in a safe, secure and confidential manner. >> Unauthorized redisclosure or failure to maintain confidentiality may subject >> you to federal and state penalties. If you are not the intended recipient, >> please immediately notify us by return email, and delete this message from >> your computer. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > From aj.taylor <@t> blueyonder.co.uk Wed Nov 25 08:37:08 2009 From: aj.taylor <@t> blueyonder.co.uk (alan taylor) Date: Wed Nov 25 08:36:58 2009 Subject: [Histonet] In need of light bulbs References: Message-ID: Hi Jim Although we are based in the UK we obtain our replacement bulbs from a company in Chicago called Topbulb. They have a huge range of lamps and bulbs for microscopes, lab equipment and medical equipment. Their prices are competitive and they offer a quick service. I'm sure that if you gave them your model number details their staff would be able to help you. I must add that I have no interest in this lamp supply company. Happy hunting and good luck. Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter Devon. EX1 3AJ. UK Tel: 044 (0)1392 660132 ----- Original Message ----- From: "jstaruk" To: Sent: Tuesday, November 24, 2009 10:09 PM Subject: [Histonet] In need of light bulbs Does anyone know what size bulb fits the Shandon embedding station, model #64000004? There are two of them at the spigot. Both of mine are burnt out and neither have any numbers on the glass or base! Gracias Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NLinke <@t> mednet.ucla.edu Wed Nov 25 08:37:51 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Wed Nov 25 08:37:59 2009 Subject: [Histonet] Sakura Embedding In-Reply-To: <4B0D4032.5040604@pathology.washington.edu> Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE4968CDAC2@EMGMB1.ad.medctr.ucla.edu> That was my concern...leaving the orientation of tissues up to grossing personnel, residents and other unsavory folks doesn't sound like a wise idea to me.... Noelle -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Wednesday, November 25, 2009 6:33 AM To: Anne van Binsbergen Cc: Linke, Noelle; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Embedding As I recall you don't have to do anything special at the time of embedding, but what is it like for the person grossing? They have to correctly orientate the tissue or the specimen could be ruined? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Anne van Binsbergen wrote: > i am - love it!! > > 2009/11/25 Linke, Noelle > > >> Is anyone using the Sakura TissueTek AutoTec automated embedding machine in >> their lab? Do you like it? >> >> Thank you! >> Noelle >> >> No?lle Linke M.S., HTL(ASCP)QIHC >> Manager, Histology Services >> Department of Pathology & Laboratory Medicine >> David Geffen School of Medicine at UCLA >> Phone: 310-825-7397 >> Pager: 97471 >> nlinke@mednet.ucla.edu >> >> >> >> >> >> >> ________________________________ >> IMPORTANT WARNING: This email (and any attachments) is only intended for >> the use of the person or entity to which it is addressed, and may contain >> information that is privileged and confidential. You, the recipient, are >> obligated to maintain it in a safe, secure and confidential manner. >> Unauthorized redisclosure or failure to maintain confidentiality may subject >> you to federal and state penalties. If you are not the intended recipient, >> please immediately notify us by return email, and delete this message from >> your computer. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From Robin.Taylor <@t> prexushealth.com Wed Nov 25 09:13:51 2009 From: Robin.Taylor <@t> prexushealth.com (Taylor, Robin) Date: Wed Nov 25 09:15:09 2009 Subject: [Histonet] (no subject) Message-ID: <884A8E132D88314EA5E1FC3BBCA3DA2E5ADA674961@PHPEXMBCLUS.docsgroup.com> We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From victor <@t> pathology.washington.edu Wed Nov 25 09:16:47 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Nov 25 09:17:18 2009 Subject: [Fwd: RE: [Histonet] Sakura Embedding] Message-ID: <4B0D4A5F.3020009@pathology.washington.edu> Wanted to share this reply. -------- Original Message -------- Subject: RE: [Histonet] Sakura Embedding Date: Wed, 25 Nov 2009 09:56:56 -0500 From: Durden, Kelley To: 'Victor Tobias' References: <0C96F0BFE078D74C91A1C541D24A6AE4968CDA33@EMGMB1.ad.medctr.ucla.edu> <4B0D4032.5040604@pathology.washington.edu> Hey Victor, Just went out to CA last month for Sakura training on the VIP 6 and got to look at the "dream lab" Sakura just set up. Very cool! At that lab they have the Automatic Embedding system. What they do to get proper orientation at the time of grossing is they've developed special cassettes that have, for lack of a better word, baskets. They have a biopsy cassette basket, a basket that could be used for tubular structures (ie vas deferens), they have a basket for larger specimens (uterus etc) and baskets that have rows for breast bx and prostate bx. The whole idea is to maintain orientation all the way through the process. The tissues are oriented in these cassette baskets at the time of grossing. They are loaded onto the processor, then loaded into the auto embedding center. Never having to re orient the samples. Then the baskets are embedded directly into the paraffin wax and are sectioned. You section right through the basket. It is made of a special type of plastic that is "sectionable." It is a really cool idea and process to watch. I brought home some samples of the baskets so I could try them here in our lab even though we don't have the auto embedding station. We sectioned through a couple of the different basket varieties and got good results. I'd contact my Sakura rep for some samples so you could try to section with the basket and see if it works for you. We don't have the volume that would necessitate an auto embedder b/c we are a research lab - but if we could justify it I'd love to have it. On another note - we love our VIP 6 processor and the training they sent me for was phenomenal. Hope this helps! Kelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, November 25, 2009 9:33 AM To: Anne van Binsbergen Cc: histonet@lists.utsouthwestern.edu; Linke, Noelle Subject: Re: [Histonet] Sakura Embedding As I recall you don't have to do anything special at the time of embedding, but what is it like for the person grossing? They have to correctly orientate the tissue or the specimen could be ruined? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Anne van Binsbergen wrote: > i am - love it!! > > 2009/11/25 Linke, Noelle > > >> Is anyone using the Sakura TissueTek AutoTec automated embedding machine in >> their lab? Do you like it? >> >> Thank you! >> Noelle >> >> No?lle Linke M.S., HTL(ASCP)QIHC >> Manager, Histology Services >> Department of Pathology & Laboratory Medicine >> David Geffen School of Medicine at UCLA >> Phone: 310-825-7397 >> Pager: 97471 >> nlinke@mednet.ucla.edu >> >> >> >> >> >> >> ________________________________ >> IMPORTANT WARNING: This email (and any attachments) is only intended for >> the use of the person or entity to which it is addressed, and may contain >> information that is privileged and confidential. You, the recipient, are >> obligated to maintain it in a safe, secure and confidential manner. >> Unauthorized redisclosure or failure to maintain confidentiality may subject >> you to federal and state penalties. If you are not the intended recipient, >> please immediately notify us by return email, and delete this message from >> your computer. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From 41dmb41 <@t> gmail.com Wed Nov 25 09:29:44 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Nov 25 09:30:07 2009 Subject: [Histonet] (no subject) In-Reply-To: <884A8E132D88314EA5E1FC3BBCA3DA2E5ADA674961@PHPEXMBCLUS.docsgroup.com> References: <884A8E132D88314EA5E1FC3BBCA3DA2E5ADA674961@PHPEXMBCLUS.docsgroup.com> Message-ID: I'd be curious to see how you have your processor times set for each station. I'm sure you're using a different setting on the weekends to account for the longer time. Have you made sure that all the other stations are getting the same time they normally get during the week? Drew On Wed, Nov 25, 2009 at 10:13, Taylor, Robin wrote: > We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially ?breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. > Robin Taylor HT > Butler County Medical Center > Hamilton, OH > > > ________________________________ > This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Wed Nov 25 09:56:53 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Nov 25 09:56:58 2009 Subject: [Histonet] Biopsy fixation In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0736E70D@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835D09@LSRIEXCH1.lsmaster.lifespan.org> 70% ethanol is the best solution for any kind of long term post-fixation storage of tissue samples. Tissues can be left in it essentially indefinitely. PBS has no preservative properties, doesn't prevent growth of microorganisms, and should be used for short term storage only, and then only with refrigeration. From mpence <@t> grhs.net Wed Nov 25 10:03:46 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Nov 25 10:03:51 2009 Subject: [Histonet] (no subject) In-Reply-To: <884A8E132D88314EA5E1FC3BBCA3DA2E5ADA674961@PHPEXMBCLUS.docsgroup.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CA8@is-e2k3.grhs.net> Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Nov 25 10:28:15 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Nov 25 10:28:26 2009 Subject: [Histonet] (no subject) In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3CA8@is-e2k3.grhs.net> References: <884A8E132D88314EA5E1FC3BBCA3DA2E5ADA674961@PHPEXMBCLUS.docsgroup.com> <661949901A768E4F9CC16D8AF8F2838C017A3CA8@is-e2k3.grhs.net> Message-ID: <4B0D14CE.7400.0077.1@harthosp.org> I keep hearing that the "guidelines" will be changed to "6-72 hours". Therefore, I wouldn't make any changes right now unless you are getting suboptimal results. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Mike Pence" 11/25/2009 11:03 AM >>> Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Nov 25 11:00:34 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Nov 25 11:00:39 2009 Subject: [Histonet] (no subject) Message-ID: <57BE698966D5C54EAE8612E8941D76830767B84E@EXCHANGE3.huntingtonhospital.com> We don't work on Saturdays, either. Our pathologists come in on Sunday to gross, so they take the breast specimens off of the processor and place them in a plastic container. On Monday morning, we melt them down and embed and cut them. We have three processors and one is set up for breast and/or fatty specimens. I have a separate program for the weekend. I extend the times in all of the solutions, especially xylene and paraffin, to improve the overall processing of the breast specimens. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, November 25, 2009 8:04 AM To: Taylor, Robin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Nov 25 11:30:27 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 25 11:30:33 2009 Subject: AW: [Histonet] Biopsy fixation In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0736E70D@NTRSEVS30002.s3.ms.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C0736E70D@NTRSEVS30002.s3.ms.unilever.com> Message-ID: Kris, why don't you leave the skin biopsies in NBF over night? At our institute it is usual practice to let this specimens fix until next day, if they arive after 1 p.m. I cannot see a negative effect. For me 4 hours in formalin are too less for a good fixation. How long is the duration of fixation in the infiltration processor? Aren't the biopsies rather brittle while cutting, due to secondary fixation with ethanol? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kalleberg, Kristopher Gesendet: Mittwoch, 25. November 2009 00:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Biopsy fixation Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is the maximum time we can leave the biopsies in 70% alcohol before processing into paraffin? Is there a better reagent, such as PBS, other than alcohol to leave the biopsy in for a few days before tissue processing. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Nov 25 12:13:10 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Nov 25 12:14:59 2009 Subject: [Histonet] In need of light bulbs References: Message-ID: Yes! Heaven forbid we don't need any dim bulbs in the lab. :) Sorry couldn't resist. Happy Turkey day everyone. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of alan taylor Sent: Wed 11/25/2009 8:37 AM To: jstaruk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] In need of light bulbs Hi Jim Although we are based in the UK we obtain our replacement bulbs from a company in Chicago called Topbulb. They have a huge range of lamps and bulbs for microscopes, lab equipment and medical equipment. Their prices are competitive and they offer a quick service. I'm sure that if you gave them your model number details their staff would be able to help you. I must add that I have no interest in this lamp supply company. Happy hunting and good luck. From tkngflght <@t> yahoo.com Wed Nov 25 13:34:10 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Nov 25 13:34:15 2009 Subject: [Histonet] weekend proc. fix -- a simple idea Message-ID: <384497.20834.qm@web50910.mail.re2.yahoo.com> hi Robin- ? IThere are a dozen ways to address this but a very simple one without extra resources might be this: ? Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing.? 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time.? ? All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. ? Happy Thanksgiving! ? Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 From Laura.Miller <@t> leica-microsystems.com Wed Nov 25 16:01:54 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Wed Nov 25 16:02:35 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 11/24/2009 and will not return until 11/30/2009. I will be on vacation until Monday, November 30th. I will respond to your email when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From pruegg <@t> ihctech.net Wed Nov 25 16:15:05 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 25 16:16:04 2009 Subject: SPAM-LOW: RE: [Histonet] (no subject) In-Reply-To: <4B0D14CE.7400.0077.1@harthosp.org> References: <884A8E132D88314EA5E1FC3BBCA3DA2E5ADA674961@PHPEXMBCLUS.docsgroup.com><661949901A768E4F9CC16D8AF8F2838C017A3CA8@is-e2k3.grhs.net> <4B0D14CE.7400.0077.1@harthosp.org> Message-ID: <9715A5F4F1E24091A963BA2C8F475C02@prueggihctechlt> Are they cutting the pieces extra thick because they are sitting over the weekend, perhaps? Even if the tissues are well fixed if they are too thick they will not process well, especially breast fat. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, November 25, 2009 9:28 AM To: Mike Pence; histonet@lists.utsouthwestern.edu; Robin Taylor Subject: SPAM-LOW: RE: [Histonet] (no subject) I keep hearing that the "guidelines" will be changed to "6-72 hours". Therefore, I wouldn't make any changes right now unless you are getting suboptimal results. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Mike Pence" 11/25/2009 11:03 AM >>> Side note question - How are you dealing with the timing issues with breast specimens being fixed in formalin for greater than 48 hr in regard to her-2 requirements with not working Saturdays? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Robin Sent: Wednesday, November 25, 2009 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 25 16:20:58 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 25 16:21:48 2009 Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea In-Reply-To: <384497.20834.qm@web50910.mail.re2.yahoo.com> References: <384497.20834.qm@web50910.mail.re2.yahoo.com> Message-ID: <30509A42F06441B8AD3119B92DD7590E@prueggihctechlt> This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- ? IThere are a dozen ways to address this but a very simple one without extra resources might be this: ? Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing.? 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time.? ? All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. ? Happy Thanksgiving! ? Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 25 16:35:17 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 25 16:36:08 2009 Subject: SPAM-LOW: [Histonet] Biopsy fixation In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0736E70D@NTRSEVS30002.s3.ms.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C0736E70D@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <14C1144C99F14591A77B2221A6059AFB@prueggihctechlt> You can leave it in formalin for 48-72 hours without a problem, it might be better than fixing just for 4 hours, some say adequate formalin fixation takes at least 24 hours no matter the size of the sample. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, November 24, 2009 4:51 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Biopsy fixation Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is the maximum time we can leave the biopsies in 70% alcohol before processing into paraffin? Is there a better reagent, such as PBS, other than alcohol to leave the biopsy in for a few days before tissue processing. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Thu Nov 26 01:52:43 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Nov 26 01:53:04 2009 Subject: [Histonet] RE: difference between immunocytochemistry and immunohistochemistry-Fr Message-ID: Hi,Good point you are raising here! Despite tons of literature mixing up the terms IHC and ICC (especially coming from the UK), I do believe there is a very big difference here. Like you described, we also encountered a situation that IFNgamma antibody worked very well in ICC (after fixing the cells in 4% PFA and using saponin 0.1% to open-up the cell membranes to get the cytoplasmic cytokine stained) but absolutely not with IHC on cryo sections. After some experiments we concluded that IFNgamma leaks away from the tissue sections during fixation (either acetone or 4% PFA). We also concluded that findings like this demonstrate we better do not mix up IHC and ICC! For example, if a company notes about one of their antibodies that it is applicable for IHC, but they mean staining of intact cells, consequently one may have a problem using that antibody on tissue tissue sections. In my lectures I always mention that we better use the terms IHC and I CC as they are originally meant for: IHC for staining tissue sections (cryo, paraffin or plastic) and ICC for staining intact cells. I send you a pdf of our paper to your private e-mail address. Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: c.m.vanderloos@amc.uva.nl Date: Tue, 24 Nov 2009 21:00:40 -0800 (PST) From: Dong Yujuan Subject: [Histonet] difference between immunocytochemistry and immunohistochemistry-Fr To: histonet@lists.utsouthwestern.edu Hi all, I am using an monoclonal antibody to test a membrane protein (tested appilication include Flow cyt and western blot). It??was succsessfuly used in Immunocytochemistry (cell culture) before. However when I applied it in Immunohistochemistry (Frozen sections, human tissue), there was no signal at all (The fixation is the same). Is there so big difference between ICC and IHC-Fr? Is it my problem or the antibody's? Many thanks! ?? Celia Chinese University of Hong Kong Hong Kong From Andrew.Prior <@t> Smith-Nephew.com Thu Nov 26 02:54:49 2009 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu Nov 26 02:55:16 2009 Subject: [Histonet] Sanderson's Rapid Bone Stain - New supplier found Message-ID: <6C18ADDF244BF8439412C063019CFFEC0BE0ABA9@EHS021.wound.san> Hi all, A couple of weeks ago I posted a message about needing a new supplier for Sanderson's RBS now that Surgipath/Leica have stopped selling it. I've just had confirmation that Dorn & Hart Microedge, Inc in Chicago will be taking over the manufacture and supply of Sanderson's RBS. Stain production has just started and should be available from mid December. Good news for those of us working with resin sections. Thanks to everyone who got in touch and offered suggestions and recipes. Andrew Andrew Prior Histologist Smith &Nephew Research Centre York UK Andrew.Prior@smith-nephew.com Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From tifei <@t> foxmail.com Thu Nov 26 08:26:42 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Nov 26 08:26:54 2009 Subject: [Histonet] IBA1-microglia staining on frozen sections Message-ID: <200911262226410753567@foxmail.com> Hi all, i just can not get this IBA1 (marker of microglia) well stained on my frozen sections fixed with acetone (sections were either dried up in air for 1 hour or fixed immediately). But I always get good staining on PFA-perfused brain tissue. Will IBA1 antigen reactivity lose in acetone fixation? 2009-11-26 TF From tifei <@t> foxmail.com Thu Nov 26 10:59:11 2009 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Thu Nov 26 11:01:28 2009 Subject: [Histonet] IBA1-microglia staining on frozen sections Message-ID: Very surprising! I just use PFA fixation after I cut the tissue, and I can stain it again! Anyone has the experience of staining IBA1 on alcohol/acetone-fixed brain sections before? In literature it said yes this works. ------------------ Original ------------------ From: "TF"; Date: Thu, Nov 26, 2009 10:26 PM To: "Histonet@lists.utsouthwestern.edu"; Subject: [Histonet] IBA1-microglia staining on frozen sections Hi all, i just can not get this IBA1 (marker of microglia) well stained on my frozen sections fixed with acetone (sections were either dried up in air for 1 hour or fixed immediately). But I always get good staining on PFA-perfused brain tissue. Will IBA1 antigen reactivity lose in acetone fixation? 2009-11-26 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Thu Nov 26 11:15:25 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Nov 26 11:15:30 2009 Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 25 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01143171@mail.pcnm.com> ------------------------------ Are you already certified to practice in Canada? If not, you will need to contact the CSMLS (Canadian Society of Medical Laboratory Sciences). They can guide you on how to go about getting Canadian certification (which you must have to practice in Canada). If you already do, I believe each province has its own society with websites that post jobs that you can check out. If you are American, you would have to contact immigration to see what paperwork is required to get some kind of working visa. Hope this helps. Joanne Clark, HT, MLT (Canadian certification) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM Message: 3 Date: Fri, 20 Nov 2009 17:36:31 -0800 (PST) From: Ade Ade Subject: [Histonet] HISTOLOGY JOB IN CANADA To: histonet@lists.utsouthwestern.edu Message-ID: <784992.3124.qm@web113919.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? ??? Hi, ?????? I am an histotechnologist currently practicing in the United States. I am looking for infos on how to get? job as either Histotechnologist or IHC-Tech in Canada. ?? I will be very grateful, if you guys could furnish me with all the necessary infos. ? Thanking you all for your usual cooperation. ? ? Ade Ade. From Maxim_71 <@t> mail.ru Thu Nov 26 13:00:05 2009 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Nov 26 13:00:14 2009 Subject: [Histonet] Biopsy fixation Message-ID: <1837766043.20091126220005@mail.ru> Kristopher: We did and still does next things: 1. Fixation in 10% NBF not less than 24 hours at RT or at 37-40oC overnight (from 16 PM to 8 AM). 2. Rinse with tap water 15-20 mins at RT. 3. Keep in 70% isopropanol at RT before processing as need long without any adverse effects. We totally excluded ethanol as dhydratant. If you need our processing schedule, please let me know. All the best, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From hymclab.hymclab <@t> ministryhealth.org Fri Nov 27 10:18:10 2009 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Fri Nov 27 10:18:26 2009 Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea In-Reply-To: <30509A42F06441B8AD3119B92DD7590E@prueggihctechlt> References: <384497.20834.qm@web50910.mail.re2.yahoo.com> <30509A42F06441B8AD3119B92DD7590E@prueggihctechlt> Message-ID: I know of a lab that was dinged by CAP for doing this. The inspector said that was not a suitable solution to the problem. The lab had to change their scheduling patterns and have someone come in and embed the tissues on Saturday am. This was just one instance that I heard of and was probably the inspector's personal opinion. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 25, 2009 4:21 PM To: 'Cheryl'; Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing. 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Fri Nov 27 11:09:03 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 27 11:09:08 2009 Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea In-Reply-To: Message-ID: <729922.15177.qm@web65701.mail.ac4.yahoo.com> Usually is the inspector's (also usually unqualified ) opinion. Just the "adrenaline rush" of citing somebody. Ren? J. --- On Fri, 11/27/09, hymclab wrote: From: hymclab Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea To: "'Patsy Ruegg'" , "'Cheryl'" , "Robin.Taylor@prexushealth.com" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, November 27, 2009, 11:18 AM I know of a lab that was dinged by CAP for doing this.? The inspector said that was not a suitable solution to the problem.? The lab had to change their scheduling patterns and have someone come in and embed the tissues on Saturday am. This was just one instance that I heard of and was probably the inspector's personal opinion. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 25, 2009 4:21 PM To: 'Cheryl'; Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing.? 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Nov 27 11:11:41 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Nov 27 11:11:47 2009 Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea In-Reply-To: <729922.15177.qm@web65701.mail.ac4.yahoo.com> Message-ID: Do you know if the lab that was cited, appealed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, November 27, 2009 12:09 PM To: 'Patsy Ruegg'; 'Cheryl'; Robin.Taylor@prexushealth.com; hymclab Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea Usually is the inspector's (also usually unqualified ) opinion. Just the "adrenaline rush" of citing somebody. Ren? J. --- On Fri, 11/27/09, hymclab wrote: From: hymclab Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea To: "'Patsy Ruegg'" , "'Cheryl'" , "Robin.Taylor@prexushealth.com" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, November 27, 2009, 11:18 AM I know of a lab that was dinged by CAP for doing this.? The inspector said that was not a suitable solution to the problem.? The lab had to change their scheduling patterns and have someone come in and embed the tissues on Saturday am. This was just one instance that I heard of and was probably the inspector's personal opinion. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 25, 2009 4:21 PM To: 'Cheryl'; Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing.? 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From hymclab.hymclab <@t> ministryhealth.org Fri Nov 27 13:27:01 2009 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Fri Nov 27 13:27:17 2009 Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea In-Reply-To: References: <729922.15177.qm@web65701.mail.ac4.yahoo.com> Message-ID: No, I think they just stopped leaving in 70% alcohol and changed their process. Dawn -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, November 27, 2009 11:12 AM To: Rene J Buesa; Patsy Ruegg; Cheryl; Robin.Taylor@prexushealth.com; hymclab Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea Do you know if the lab that was cited, appealed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, November 27, 2009 12:09 PM To: 'Patsy Ruegg'; 'Cheryl'; Robin.Taylor@prexushealth.com; hymclab Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea Usually is the inspector's (also usually unqualified ) opinion. Just the "adrenaline rush" of citing somebody. Ren? J. --- On Fri, 11/27/09, hymclab wrote: From: hymclab Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea To: "'Patsy Ruegg'" , "'Cheryl'" , "Robin.Taylor@prexushealth.com" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, November 27, 2009, 11:18 AM I know of a lab that was dinged by CAP for doing this. The inspector said that was not a suitable solution to the problem. The lab had to change their scheduling patterns and have someone come in and embed the tissues on Saturday am. This was just one instance that I heard of and was probably the inspector's personal opinion. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 25, 2009 4:21 PM To: 'Cheryl'; Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing. 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From pruegg <@t> ihctech.net Fri Nov 27 13:30:55 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 27 13:31:37 2009 Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea In-Reply-To: References: <729922.15177.qm@web65701.mail.ac4.yahoo.com> Message-ID: <45DE1B44C497461485326E69777438B3@ihctechq9h2qof> Cap uses a term called "conventional processing" which is not well defined and at the interruption of the inspector, but I have been told that tissue processing that is not "conventional" (taking the tissues out of the wax and letting them harden after process and wait a few days to embed, or leaving the tissue in 70% alcohol on the processor for 48 hours may be interpreted by some inspectors as "not conventional" processing) and in that case you would need to do validation studies showing that the results are as good as "conventional" 48 hour processing when these deviations are not used. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, November 27, 2009 10:12 AM To: Rene J Buesa; Patsy Ruegg; Cheryl; Robin.Taylor@prexushealth.com; hymclab Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea Do you know if the lab that was cited, appealed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, November 27, 2009 12:09 PM To: 'Patsy Ruegg'; 'Cheryl'; Robin.Taylor@prexushealth.com; hymclab Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea Usually is the inspector's (also usually unqualified ) opinion. Just the "adrenaline rush" of citing somebody. Ren? J. --- On Fri, 11/27/09, hymclab wrote: From: hymclab Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea To: "'Patsy Ruegg'" , "'Cheryl'" , "Robin.Taylor@prexushealth.com" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, November 27, 2009, 11:18 AM I know of a lab that was dinged by CAP for doing this.? The inspector said that was not a suitable solution to the problem.? The lab had to change their scheduling patterns and have someone come in and embed the tissues on Saturday am. This was just one instance that I heard of and was probably the inspector's personal opinion. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 25, 2009 4:21 PM To: 'Cheryl'; Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea This would work but make sure your tissue is very well fixed 24-48hrs before letting them sit in 70% or they will get alcohol fixed and that can be a problem. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, November 25, 2009 12:34 PM To: Robin.Taylor@prexushealth.com Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] weekend proc. fix -- a simple idea hi Robin- IThere are a dozen ways to address this but a very simple one without extra resources might be this: Set your processor with the extra 24-48 hours held in the first alcohol. You probably don't want to spread the time across the whole machine or your end product will be VERY different than your weekly processing.? 70% doesn't harden the biopsies and meets the fixation requirements currently under regulations. This works if you don't add to the processors after they're first loaded--set your end time for Monday morning and go! Once they change the reg to 6-72 hours, then rethink the distribution of time. All the other solutions are valid, but his way you don't have to obligate the pathologists to extra handling or have to deal with more than one processor and it's still inside current regs. Happy Thanksgiving! Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. 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Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From Maxim_71 <@t> mail.ru Fri Nov 27 15:28:55 2009 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Nov 27 15:29:03 2009 Subject: [Histonet] Undelivered Mail Returned to Sender Message-ID: <1887068226.20091128002855@mail.ru> Rene, I am sorry, that I need call to you through Histonet, but, it seems that yahoo-server have some troubles with my mail-delivery. I got next message now: > This is the mail system at host fallback2.mail.ru. > > I'm sorry to have to inform you that your message could not > be delivered to one or more recipients. It's attached below. > > For further assistance, please send mail to > > If you do so, please include this problem report. You can > delete your own text from the attached returned message. > > The mail system > > : delivery temporarily suspended: host > g.mx.mail.yahoo.com[98.137.54.238] refused to > talk to me: 421 4.7.0 [TS02] > Messages from 94.100.176.87 temporarily deferred due to user complaints - > 4.16.56.1; see > http://postmaster.yahoo.com/421-ts02.html I hope, that these troubles are temporary. I successfully received both, word- and pdf-documents and works with they since 25 November 2009. Sincerely, Maxim. mailto:Maxim_71@mail.ru From syinan <@t> ucalgary.ca Fri Nov 27 16:44:17 2009 From: syinan <@t> ucalgary.ca (Salim Yalcin Inan) Date: Fri Nov 27 16:44:57 2009 Subject: [Histonet] a basic question about immunohistochemistry In-Reply-To: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA> References: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA> Message-ID: Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syinan@ucalgary.ca From jkiernan <@t> uwo.ca Sat Nov 28 00:33:55 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 28 00:33:59 2009 Subject: [Histonet] a basic question about immunohistochemistry In-Reply-To: References: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA> Message-ID: Dear Dr Inan, Anyone can fix, process and do immunohistochemistry on parts of old, dead "lab" rats. What you see will have to be compared with comparably immunostained sections of old, dead "normal" rat tissues. This is bad science! You need to repeat the experiment. Do the work yourself and don't rely on untrained or uneducated "staff". John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Salim Yalcin Inan Date: Friday, November 27, 2009 17:46 Subject: [Histonet] a basic question about immunohistochemistry To: histonet@lists.utsouthwestern.edu > Dear All, > > Because I am new in immunohistochemistry, I have a basic > question about it. > What if your rat dies in the evening or in the weekend, which > you are doing > a chronic experiment and need to collect brain tissue for > immunohistochemistry? And let's say, the staff did not noticed > it to inform > you on time. Several hours passed since your rat died. There is > no way to do > perfusion. Is it still possible to do immunohistochemistry? > Thank you very much in advance. > > Best regards, > > Salim Yalcin Inan, Ph.D. > (post-doctoral fellow) > Department of Clinical Neurosciences > University of Calgary > syinan@ucalgary.ca > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sweething63 <@t> msn.com Sat Nov 28 09:47:43 2009 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Sat Nov 28 09:47:47 2009 Subject: [Histonet] a basic question about immunohistochemistry In-Reply-To: References: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA>, , Message-ID: John, OUCH! > From: jkiernan@uwo.ca > To: syinan@ucalgary.ca > Date: Sat, 28 Nov 2009 01:33:55 -0500 > Subject: Re: [Histonet] a basic question about immunohistochemistry > CC: histonet@lists.utsouthwestern.edu > > Dear Dr Inan, > > Anyone can fix, process and do immunohistochemistry on parts of old, dead "lab" rats. What you see will have to be compared with comparably immunostained sections of old, dead "normal" rat tissues. This is bad science! > > You need to repeat the experiment. Do the work yourself and don't rely on untrained or uneducated "staff". > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Salim Yalcin Inan > Date: Friday, November 27, 2009 17:46 > Subject: [Histonet] a basic question about immunohistochemistry > To: histonet@lists.utsouthwestern.edu > > > Dear All, > > > > Because I am new in immunohistochemistry, I have a basic > > question about it. > > What if your rat dies in the evening or in the weekend, which > > you are doing > > a chronic experiment and need to collect brain tissue for > > immunohistochemistry? And let's say, the staff did not noticed > > it to inform > > you on time. Several hours passed since your rat died. There is > > no way to do > > perfusion. Is it still possible to do immunohistochemistry? > > Thank you very much in advance. > > > > Best regards, > > > > Salim Yalcin Inan, Ph.D. > > (post-doctoral fellow) > > Department of Clinical Neurosciences > > University of Calgary > > syinan@ucalgary.ca > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Nov 28 10:09:26 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Nov 28 10:09:30 2009 Subject: [Histonet] a basic question about immunohistochemistry In-Reply-To: Message-ID: <113481.77941.qm@web65702.mail.ac4.yahoo.com> I am afraid that any IHC that you will attempt to do will not serve for your original experimental purposes of having a perfused brain. I am afraid you will have to start all over again. At least now you know a probable survival time, so start the new experiment in a way that the rat is "supposed to die" from a Tuesday on (during the work schedule). Ren? J. --- On Fri, 11/27/09, Salim Yalcin Inan wrote: From: Salim Yalcin Inan Subject: [Histonet] a basic question about immunohistochemistry To: histonet@lists.utsouthwestern.edu Date: Friday, November 27, 2009, 5:44 PM Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syinan@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Sat Nov 28 12:17:11 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Nov 28 12:17:18 2009 Subject: [Histonet] a basic question about immunohistochemistry References: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA> Message-ID: <90354A475B420441B2A0396E5008D4965E30A8@copc-sbs.COPC.local> Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syinan@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From njblademaster <@t> gmail.com Sat Nov 28 16:35:31 2009 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Sat Nov 28 16:35:56 2009 Subject: AW: [Histonet] Biopsy fixation Message-ID: <5a7745af0911281435j339ff9d3ja8d7eff31e93167b@mail.gmail.com> Gudrun I work in a dermatopathology lab, and we don't see any issues leaving our specimens in 10% NBF for days at a time. We don't work on the weekends, so any specimens that are grossed remain on our processors in NBF from Friday at 8:00 PM until about 5:30 PM on Sunday. I also agree with Kris that 4 hours of fixation is not long enough to ensure proper fixation unless they are shave biopsies. Nate From pruegg <@t> ihctech.net Sat Nov 28 16:55:24 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Nov 28 16:56:15 2009 Subject: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry In-Reply-To: <90354A475B420441B2A0396E5008D4965E30A8@copc-sbs.COPC.local> References: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA> <90354A475B420441B2A0396E5008D4965E30A8@copc-sbs.COPC.local> Message-ID: Of course you can always do IHC on your rat that died, you just have to note that that rat died and was not profuse fixed so that the review of the results will take that in consideration, hopefully you have another rat that besides dyeing and not getting profuse fixed had all other conditions the mostly the same so you could use the properly treated rat as a standard to compare the dead rat too. Good luck and do keep asking for help at this forum, most of us will offer you our experience without judging your science. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, November 28, 2009 11:17 AM To: Salim Yalcin Inan Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syinan@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Nov 29 04:18:52 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Nov 29 04:19:00 2009 Subject: AW: [Histonet] Biopsy fixation In-Reply-To: <5a7745af0911281435j339ff9d3ja8d7eff31e93167b@mail.gmail.com> References: <5a7745af0911281435j339ff9d3ja8d7eff31e93167b@mail.gmail.com> Message-ID: <355415E1E34E48EE854F2D0A668F81E4@dielangs.at> Nate, I think you have confused the persons. I'm the one, who recommended longer fixation and Kris had made the original question. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Nathan Jentsch Gesendet: Samstag, 28. November 2009 23:36 An: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] Biopsy fixation Gudrun I work in a dermatopathology lab, and we don't see any issues leaving our specimens in 10% NBF for days at a time. We don't work on the weekends, so any specimens that are grossed remain on our processors in NBF from Friday at 8:00 PM until about 5:30 PM on Sunday. I also agree with Kris that 4 hours of fixation is not long enough to ensure proper fixation unless they are shave biopsies. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Sun Nov 29 06:24:20 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sun Nov 29 06:24:26 2009 Subject: [Histonet] Cassette labelling Message-ID: Hi Histonetters Who out there has a Sakura iDent? I have just taken delivery of a brand new machine and I am having serious issues with the quality of the print on the cassette face - the ink rubs off with xylene!!! Yes, I leave the print to dry before I handle the cassettes No, I dont use Tissue-Tek xylene substitute - my xylene is made by Fisher I would love to know if anyone else has had similar issues Yes, Rene, this is the diehard number 1 Sakura fan having issues with a Sakura product!!! hopefully yours (pun intended) AbuDhabiAnnie -- Anne van Binsbergen (Hope) Abu Dhabi UAE From leswes <@t> shaw.ca Sun Nov 29 08:22:57 2009 From: leswes <@t> shaw.ca (Lesley Weston) Date: Sun Nov 29 08:23:37 2009 Subject: [Histonet] a basic question about immunohistochemistry In-Reply-To: <90354A475B420441B2A0396E5008D4965E30A8@copc-sbs.COPC.local> References: <20091127180053.D4CBA74007@mhub3.UCALGARY.CA> <90354A475B420441B2A0396E5008D4965E30A8@copc-sbs.COPC.local> Message-ID: Just about all biology research institutions have an animal unit with qualified technicians to look after the animals, rather than someone qualified in other areas; however the technicians are subject to human failings such as going home at night. I've never heard of a one- person research team, and I don't think it would be all that effective. The tissue from the animal that died at the wrong time is not comparable to perfused tissue, but as Tom says, it might still be worth processing. If nothing else, it will show how much difference perfusion makes. Lesley Weston. On 28-Nov-09, at 10:17 AM, Thomas Jasper wrote: > Dear Salim, > > As you have been informed, doing immunohistochemistry is possible on > this tissue. After all it's possible to do IHC on any tissue whether > the conditions you want to test under are ideal or not. > > Being chastised on this list and calling your work "bad science" is > totally out of line and certainly does not help you out. I think some > people would do well to reserve judgment, particularly when there's no > way they can fully understand what's going on with your project. > Having > worked in research myself, I completely understand that animals will > die, at the most inconvenient times, during a study. > > First of all you should incorporate the data about the animal dying > into > your study notes. Secondly, there's no harm in running the IHC on > this > animal's tissue. You can use the results comparatively with results > from some perfused tissue later on. > > I don't know Salim, some people might call it damage control, or > making > the best of a less than ideal situation. Again, I don't know exactly > what you're working on but it seems there's information worth > gathering > despite the circumstances. I also understand that it's probably > next to > impossible to carry out experiments and research alone. Having > reliable > staff assist you is not unusual either. > > Good luck to you, > > Tom Jasper > > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim > Yalcin Inan > Sent: Friday, November 27, 2009 2:44 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] a basic question about immunohistochemistry > > Dear All, > > Because I am new in immunohistochemistry, I have a basic question > about > it. > What if your rat dies in the evening or in the weekend, which you are > doing a chronic experiment and need to collect brain tissue for > immunohistochemistry? And let's say, the staff did not noticed it to > inform you on time. Several hours passed since your rat died. There is > no way to do perfusion. Is it still possible to do > immunohistochemistry? > Thank you very much in advance. > > Best regards, > > Salim Yalcin Inan, Ph.D. > (post-doctoral fellow) > Department of Clinical Neurosciences > University of Calgary > syinan@ucalgary.ca > > From rsrichmond <@t> gmail.com Sun Nov 29 12:34:36 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun Nov 29 12:34:39 2009 Subject: [Histonet] Re: a basic question about immunohistochemistry Message-ID: Thomas Jasper certainly points out an issue of politeness: >>Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out.<< I agree up to a point - but frankly, if John Kiernan called my work "bad science" I would seriously reconsider what I was doing. (Which on occasion he has, come to think of it.) Bob Richmond Samurai Pathologist Knoxville TN From napoli <@t> siscom.net Sun Nov 29 12:53:59 2009 From: napoli <@t> siscom.net (napoli@siscom.net) Date: Sun Nov 29 12:54:03 2009 Subject: [Histonet] bone fixation and decal question Message-ID: <4b12c347.1ed.ef1.1648215162@siscom.net> Question regarding fresh tissue, in this case bone. Scenario: Mohs surgeon removes layers of skin on a patient's scalp, eventually discovering the basal cell carcinoma extends to the periosteum and so a bit of bone is taken and tested to ensure the margins are free of cancer. The only problem is that since it is bone, in order to cut it is needs to be decalcified. In order to decalcify this tissue in order to cut it either in cryostat or on regular microtome, it of course must be fixed, correct? Reason being is I have heard of Mohs dermatologic surgeons taking bones frags, letting their histotech put the fresh bone in hydrochloric acid solution for decal overnight and then cutting it in the morning on the cryostat. This seems flawed to me as the acid would seem to destroy architecture and critical proteins? Isnt this practice flawed as it doesnt allow fixation? Does the hydrochloric solution have fixative properties? Does decal halt autolysis? Also, is there any real reason why formalin fixed tissues cannot be mounted in OCT and cut frozen? Would it hurt the frozen section for the specimen to be placed into one of the decals i have seen that are both fixative and decal? (Like Surgipath Decal I)It is said to work reasonably quickly and the bone would be thin. Seems like this would be the thourough way of getting quick intraoperative results with a bit of bone on cryo. Any comments? thanks in advance From rjbuesa <@t> yahoo.com Sun Nov 29 13:19:09 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Nov 29 13:19:13 2009 Subject: [Histonet] bone fixation and decal question In-Reply-To: <4b12c347.1ed.ef1.1648215162@siscom.net> Message-ID: <387410.41916.qm@web65713.mail.ac4.yahoo.com> You answered most of your own questions. The bone should be fixed before decalcifying it. After that it could be cut in the cryostat, but why to do that? It will be quite difficult. The best solution is to fix ? decalcify ? process routine by embedding in paraffin ? cut "conventional" sections. The whole idea behind the FS is the speed of the "intraoperative" diagnosis.. Once that is lost (by fixing ? decalcifying) I don't think it matter much waiting a little bit more. Hydrochloric acid does not fix (answering to one of your questions). Ren? J. --- On Sun, 11/29/09, napoli@siscom.net wrote: From: napoli@siscom.net Subject: [Histonet] bone fixation and decal question To: histonet@lists.utsouthwestern.edu Date: Sunday, November 29, 2009, 1:53 PM Question regarding fresh tissue, in this case bone. Scenario: Mohs surgeon removes layers of skin on a patient's scalp, eventually discovering the basal cell carcinoma extends to the periosteum and so a bit of bone is taken and tested to ensure the margins are free of cancer. The only problem is that since it is bone, in order to cut it is needs to be decalcified. In order to decalcify this tissue in order to cut it either in cryostat or on regular microtome, it of course must be fixed, correct? Reason being is I have heard of Mohs dermatologic surgeons taking bones frags, letting their histotech put the fresh bone in hydrochloric acid solution for decal overnight and then cutting it in the morning on the cryostat. This seems flawed to me as the acid would seem to destroy architecture and critical proteins? Isnt this practice flawed as it doesnt allow fixation? Does the hydrochloric solution have fixative properties? Does decal halt autolysis? Also, is there any real reason why formalin fixed tissues cannot be mounted in OCT and cut frozen? Would it hurt the frozen section for the specimen to be placed into one of the decals i have seen that are both fixative and decal? (Like Surgipath Decal I)It is said to work reasonably quickly and the bone would be thin. Seems like this would be the thourough way of getting quick intraoperative results with a bit of bone on cryo. Any comments? thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dr_sadushe <@t> yahoo.com Sun Nov 29 13:22:27 2009 From: dr_sadushe <@t> yahoo.com (sadushe loxha) Date: Sun Nov 29 13:22:30 2009 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1382895317.12849077.1259522547279.JavaMail.app@ech3-cdn12.prod> LinkedIn ------------ I'd like to add you to my professional network on LinkedIn. - sadushe Confirm that you know sadushe loxha https://www.linkedin.com/e/isd/895127840/5sWEh80E/ Every day, millions of professionals like sadushe loxha use LinkedIn to connect with colleagues, find experts, and explore opportunities. ------ (c) 2009, LinkedIn Corporation From gayle.callis <@t> bresnan.net Sun Nov 29 14:15:50 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Nov 29 14:16:06 2009 Subject: Suggestions RE: [Histonet] Re: a basic question about immunohistochemistry In-Reply-To: References: Message-ID: <000c01ca7130$bfe8bbb0$3fba3310$@callis@bresnan.net> Hopefully, your next study using rats will have more than one animal. My primary research contact and his post docs along with several other research groups in our department work with rodents. Their studies are always set up with more than one animal, even pilot studies. You need to have good correlation of results and a single animal simply does not provide that correlation. Consequently, we have anywhere from 5 to 6 animals or more in any particular treatment group and the same for a control group. These animals are kept in a controlled environment animal facility during experiments under auspices of federally mandated guidelines one is required to follow or be in BIG trouble. One animal is NOT a good way to get results especially when the animal dies before a euthanasia/time endpoint for tissue collection and you need perfusion fixation as part of your protocol. This animal has fallen out of your research parameters and statistically not useful. We do not trust any IHC results from deceased animals and do not do immunostaining when this happens. When an animal has been deceased for many hours (and how would you know!), we either discard or necropsy to determine cause of death especially if infection is suspected. If more than one or more animal dies, then finding cause of death IS important before repeating the experiment if possible either overdosing of some drug, chemical agent or massive infection from infectious agents are involved in the study. We do document if an animal dies during an experiment as mortality may be an important statistic to note. IHC will more than likely be very skewed, with untrustworthy results, due to post mortem tissue autolysis affecting antigens especially if the animal has been deceased for many hours. Necropsied tissues will show autolysis too. If you use deceased experimental animal results when you need tissues correctly fixed from a euthanized animal for any manuscript destined for publication, reviewers will more than likely frown upon what you did, and reject the manuscript. You need to repeat your experiment with more animals, and also have some type of sham treatment control animal group. Bob Richmond made a good point - I would take John's comments to heart..................journal manuscript reviewers and thesis defense graduate committees can be much tougher than John is!!! Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Sunday, November 29, 2009 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: a basic question about immunohistochemistry Thomas Jasper certainly points out an issue of politeness: >>Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out.<< I agree up to a point - but frankly, if John Kiernan called my work "bad science" I would seriously reconsider what I was doing. (Which on occasion he has, come to think of it.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4647 (20091129) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4647 (20091129) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4647 (20091129) __________ The message was checked by ESET Smart Security. http://www.eset.com From gayle.callis <@t> bresnan.net Sun Nov 29 15:13:28 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Nov 29 15:13:45 2009 Subject: [Histonet] bone fixation and decal question In-Reply-To: <4b12c347.1ed.ef1.1648215162@siscom.net> References: <4b12c347.1ed.ef1.1648215162@siscom.net> Message-ID: <001301ca7138$cd4f6440$67ee2cc0$@callis@bresnan.net> The only way you can do a frozen section on undecalcified bone is using a tungsten carbide knife($12200 to $1500 per knife and need reconditioning) but you need a universal knife holder in your cryostat. Even then, doing an frozen on undecalcified fresh bone is difficult, it tends to crumble if not held together is some way. Instrumedics Cryojane (sold by Leica Microsystems) can solve this problem as you would get the margins of an intact section (soft tissue and attached bone) you need at the time of Mohs surgery. There would be no waiting for fixation, decalcification or cryoprotection into the next day. Cryojane sectioning does not require fixation or decalcification. There is a wonderful demonstration of Cryojane on http://www.alphelys.com/site/us/pHGP_CoupesCongelees.htm There is another frozen section tape method available now, and you stain the section while it is on the tape, then cover slip the tape (section side down) onto a slide. Go to this website http://section-lab.jp and read about the - Kawamoto Cryofilm method. It is less expensive than Cryojane, but does NOT transfer the section onto a slide surface - the section stays on the tape. However, transferring to the slide surface is something you may not need to do anyway since diagnosis can be made very quickly on day of Mohs surgery. This part of the Kawamoto website has references with all pdf's downloadable, http://section-lab.jp/English/Referece.htm and his original publication is there along with many others on how this Cryofilm technology is used. I am not sure if permanent mounting medias can be used with Cryofilm. Your assessment of the problem was right on. If the Surgipath Decal I is the same kind of fixative/decalcifier as is Calrite (a combination formalin/formic acid mixture) then this is a better option that protects tissue integrity with fixation at the same time as decalcifying the bone fragment. HCl or any acid used without fixing the tissue first should NEVER be done - this will macerate the tissue and bone, destroying cellular and tissue morphology. The staining will be horrible. HCl does not have fixative qualities, and any enzymes causing autolysis are certainly destroyed by the acid along with everything else in tissue - without possibility of rescuing the tissue. The practice of HCl alone is not only flawed but potentially a legal issue if the tissue/cells are destroyed by acid without using fixation first - it could be considered mishandling of a tissue sample so diagnosis isn't possible. These labs need to do some reading on working with bone fixation, decalcification, etc. and to order a good histotechnology textbook! You should cryoprotect fixed/decalcified sample with 20 to 30% sucrose before freezing or large ice crystal freezing artifact damages tissue morphology, often making tissue unrecognizable. Cryoprotection can be speeded up by vacuuming during cryoprotection plus exchanging the fixative/decalcifier should rinse the residual acid from tissue. Residual acid can damage staining and also damage metal parts in cryostat. The other option is being able to raise the fibrous periosteum without taking bone fragments - there are surgical instruments designed for doing this. Question is: is basal cell cancer also found in the bone? and that means taking the bone fragment too. Good luck Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of napoli@siscom.net Sent: Sunday, November 29, 2009 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone fixation and decal question Question regarding fresh tissue, in this case bone. Scenario: Mohs surgeon removes layers of skin on a patient's scalp, eventually discovering the basal cell carcinoma extends to the periosteum and so a bit of bone is taken and tested to ensure the margins are free of cancer. The only problem is that since it is bone, in order to cut it is needs to be decalcified. In order to decalcify this tissue in order to cut it either in cryostat or on regular microtome, it of course must be fixed, correct? Reason being is I have heard of Mohs dermatologic surgeons taking bones frags, letting their histotech put the fresh bone in hydrochloric acid solution for decal overnight and then cutting it in the morning on the cryostat. This seems flawed to me as the acid would seem to destroy architecture and critical proteins? Isnt this practice flawed as it doesnt allow fixation? Does the hydrochloric solution have fixative properties? Does decal halt autolysis? Also, is there any real reason why formalin fixed tissues cannot be mounted in OCT and cut frozen? Would it hurt the frozen section for the specimen to be placed into one of the decals i have seen that are both fixative and decal? (Like Surgipath Decal I)It is said to work reasonably quickly and the bone would be thin. Seems like this would be the thourough way of getting quick intraoperative results with a bit of bone on cryo. Any comments? thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 4647 (20091129) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 4647 (20091129) __________ The message was checked by ESET Smart Security. http://www.eset.com From gayle.callis <@t> bresnan.net Sun Nov 29 15:53:35 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Nov 29 15:53:51 2009 Subject: Correction on tungsten knife blade prices RE: [Histonet] bone fixation and decal question In-Reply-To: <001301ca7138$cd4f6440$67ee2cc0$@callis@bresnan.net> References: <4b12c347.1ed.ef1.1648215162@siscom.net> <001301ca7138$cd4f6440$67ee2cc0$@callis@bresnan.net> Message-ID: <001901ca713e$67aef230$370cd690$@callis@bresnan.net> Sorry Folks, I wrote: tungsten carbide knife ($12200 to $1500 per knife and need reconditioning)" a typo, fault of the computer obviously. Tungsten carbide knives do not cost $12200 although at times seem like they do............. pricey little things. $1200 sounds better, and there are several vendors, Dorn and Hart, DDK, and probably microtome manufacturers too. Gayle Callis __________ Information from ESET Smart Security, version of virus signature database 4647 (20091129) __________ The message was checked by ESET Smart Security. http://www.eset.com From mwfolsom <@t> swcp.com Sun Nov 29 21:37:51 2009 From: mwfolsom <@t> swcp.com (Michael W. Folsom) Date: Sun Nov 29 21:38:30 2009 Subject: [Histonet] Manual for and/or info about Sunkay Messer Glass knife Maker Type A Message-ID: <4B133E0F.1060203@swcp.com> Folks: Subject line says it all - - I'm especially interested in finding out how it works and how the glass is scored As always my thanks!!!!! Mike Folsom From yujuan_dong <@t> yahoo.com Mon Nov 30 02:42:02 2009 From: yujuan_dong <@t> yahoo.com (Dong Yujuan) Date: Mon Nov 30 02:42:14 2009 Subject: [Histonet] RE: difference between immunocytochemistry and immunohistochemistry-Fr Message-ID: <810083.46837.qm@web55502.mail.re4.yahoo.com> Dear Chris Richard and Adam, ? Thank you so much for your advice. I decide try more, e.g. change other fixing methods.?The protein is LRRC32 and only 2 companies provide Ab. Neither?of them is for IHC. If it doesn't work, I think maybe I will generate the polyclonal Ab by myself. ? C Yahoo!???????????????????! ??? http://hk.promo.yahoo.com/security/ ????! From jamie.erickson <@t> abbott.com Mon Nov 30 07:55:03 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon Nov 30 07:55:12 2009 Subject: [Histonet] Re: a basic question about immunohistochemistry (IHC ON DEAD RAT) In-Reply-To: References: Message-ID: Dear Salim, I have done lots of IHC on mouse and rat tissues ie brain and trying to make correlations on viable tissue is hard enough. If you do preform the IHC it may confound your data one way or the other and leave you with more questions then answers. My recommendation would be to leave this animal out of the analysis. Autolysis can be difficult to read through with IHC in the brain. Ultimately it is up to you but if this animal shows more or less staining and skews your average in that group you will be left with the nagging question " was it the autolysis that made the difference" My 2 cents...good luck.. Jamie E Erickson Scientist II, M.S. HTL (ASCP) Discovery Safety, Metabolism & Pharmacokinetics Abbott Laboratories From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 11/29/2009 01:02 PM Subject: Histonet Digest, Vol 72, Issue 33 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: a basic question about immunohistochemistry (Thomas Jasper) 2. AW: [Histonet] Biopsy fixation (Nathan Jentsch) 3. RE: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry (Patsy Ruegg) 4. AW: [Histonet] Biopsy fixation (Gudrun Lang) 5. Cassette labelling (Anne van Binsbergen) 6. Re: a basic question about immunohistochemistry (Lesley Weston) ---------------------------------------------------------------------- Message: 1 Date: Sat, 28 Nov 2009 10:17:11 -0800 From: "Thomas Jasper" Subject: RE: [Histonet] a basic question about immunohistochemistry To: "Salim Yalcin Inan" Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D4965E30A8@copc-sbs.COPC.local> Content-Type: text/plain; charset="us-ascii" Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syinan@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Sat, 28 Nov 2009 17:35:31 -0500 From: Nathan Jentsch Subject: AW: [Histonet] Biopsy fixation To: histonet@lists.utsouthwestern.edu Message-ID: <5a7745af0911281435j339ff9d3ja8d7eff31e93167b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Gudrun I work in a dermatopathology lab, and we don't see any issues leaving our specimens in 10% NBF for days at a time. We don't work on the weekends, so any specimens that are grossed remain on our processors in NBF from Friday at 8:00 PM until about 5:30 PM on Sunday. I also agree with Kris that 4 hours of fixation is not long enough to ensure proper fixation unless they are shave biopsies. Nate ------------------------------ Message: 3 Date: Sat, 28 Nov 2009 15:55:24 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry To: "'Thomas Jasper'" , "'Salim Yalcin Inan'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Of course you can always do IHC on your rat that died, you just have to note that that rat died and was not profuse fixed so that the review of the results will take that in consideration, hopefully you have another rat that besides dyeing and not getting profuse fixed had all other conditions the mostly the same so you could use the properly treated rat as a standard to compare the dead rat too. Good luck and do keep asking for help at this forum, most of us will offer you our experience without judging your science. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, November 28, 2009 11:17 AM To: Salim Yalcin Inan Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syinan@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 29 Nov 2009 11:18:52 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Biopsy fixation To: "'Nathan Jentsch'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <355415E1E34E48EE854F2D0A668F81E4@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Nate, I think you have confused the persons. I'm the one, who recommended longer fixation and Kris had made the original question. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Nathan Jentsch Gesendet: Samstag, 28. November 2009 23:36 An: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] Biopsy fixation Gudrun I work in a dermatopathology lab, and we don't see any issues leaving our specimens in 10% NBF for days at a time. We don't work on the weekends, so any specimens that are grossed remain on our processors in NBF from Friday at 8:00 PM until about 5:30 PM on Sunday. I also agree with Kris that 4 hours of fixation is not long enough to ensure proper fixation unless they are shave biopsies. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 29 Nov 2009 16:24:20 +0400 From: Anne van Binsbergen Subject: [Histonet] Cassette labelling To: "histonet@lists.utsouthwestern.edu" , histonet-request@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters Who out there has a Sakura iDent? I have just taken delivery of a brand new machine and I am having serious issues with the quality of the print on the cassette face - the ink rubs off with xylene!!! Yes, I leave the print to dry before I handle the cassettes No, I dont use Tissue-Tek xylene substitute - my xylene is made by Fisher I would love to know if anyone else has had similar issues Yes, Rene, this is the diehard number 1 Sakura fan having issues with a Sakura product!!! hopefully yours (pun intended) AbuDhabiAnnie -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 6 Date: Sun, 29 Nov 2009 06:22:57 -0800 From: Lesley Weston Subject: Re: [Histonet] a basic question about immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Just about all biology research institutions have an animal unit with qualified technicians to look after the animals, rather than someone qualified in other areas; however the technicians are subject to human failings such as going home at night. I've never heard of a one- person research team, and I don't think it would be all that effective. The tissue from the animal that died at the wrong time is not comparable to perfused tissue, but as Tom says, it might still be worth processing. If nothing else, it will show how much difference perfusion makes. Lesley Weston. On 28-Nov-09, at 10:17 AM, Thomas Jasper wrote: > Dear Salim, > > As you have been informed, doing immunohistochemistry is possible on > this tissue. After all it's possible to do IHC on any tissue whether > the conditions you want to test under are ideal or not. > > Being chastised on this list and calling your work "bad science" is > totally out of line and certainly does not help you out. I think some > people would do well to reserve judgment, particularly when there's no > way they can fully understand what's going on with your project. > Having > worked in research myself, I completely understand that animals will > die, at the most inconvenient times, during a study. > > First of all you should incorporate the data about the animal dying > into > your study notes. Secondly, there's no harm in running the IHC on > this > animal's tissue. You can use the results comparatively with results > from some perfused tissue later on. > > I don't know Salim, some people might call it damage control, or > making > the best of a less than ideal situation. Again, I don't know exactly > what you're working on but it seems there's information worth > gathering > despite the circumstances. I also understand that it's probably > next to > impossible to carry out experiments and research alone. Having > reliable > staff assist you is not unusual either. > > Good luck to you, > > Tom Jasper > > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim > Yalcin Inan > Sent: Friday, November 27, 2009 2:44 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] a basic question about immunohistochemistry > > Dear All, > > Because I am new in immunohistochemistry, I have a basic question > about > it. > What if your rat dies in the evening or in the weekend, which you are > doing a chronic experiment and need to collect brain tissue for > immunohistochemistry? And let's say, the staff did not noticed it to > inform you on time. Several hours passed since your rat died. There is > no way to do perfusion. Is it still possible to do > immunohistochemistry? > Thank you very much in advance. > > Best regards, > > Salim Yalcin Inan, Ph.D. > (post-doctoral fellow) > Department of Clinical Neurosciences > University of Calgary > syinan@ucalgary.ca > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 33 **************************************** From AHutton <@t> dh.org Mon Nov 30 09:20:59 2009 From: AHutton <@t> dh.org (Hutton, Allison) Date: Mon Nov 30 09:22:42 2009 Subject: [Histonet] dictation systems Message-ID: <38A56C4F4630D348A50B3720409270870744FEB3@dhmail.dhorg.org> Does anyone have any recommendations for a dictation system that can be used in the pathology lab? We prefer to continue to use the foot pedals but are willing to look into other options. Thanks for your input Allison From Vickroy.Jim <@t> mhsil.com Mon Nov 30 09:29:32 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Nov 30 09:29:38 2009 Subject: [Histonet] LOOKING FOR AN ALK-1 CONTROL BLOCK Message-ID: <24A4826E8EF0964D86BC5317306F58A5425412D956@mmc-mail.ad.mhsil.com> ANYBODY HAVE AN EXTRA ALK-1 CONTROL BLOCK THEY WOULD PART WITH? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From lblazek <@t> digestivespecialists.com Mon Nov 30 09:29:31 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Nov 30 09:30:45 2009 Subject: [Histonet] RE: dictation systems In-Reply-To: <38A56C4F4630D348A50B3720409270870744FEB3@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870744FEB3@dhmail.dhorg.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39098BE4D9AD@IBMB7Exchange.digestivespecialists.com> Hi All, I would also like to receive that info. We currently use a system called Start/Stop. It had been just fine for the past three years. Then a month ago the station where the pathologist dictates micros started to record only about every other word. I have replaced the computer, microphone foot peddle and cables. Nothing resolves the problem. The system works fine in the grossing area. Sometimes it works for about 3 cases then starts the skipping problem. If anyone has any suggestions I sure would appreciate it! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Monday, November 30, 2009 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dictation systems Does anyone have any recommendations for a dictation system that can be used in the pathology lab? We prefer to continue to use the foot pedals but are willing to look into other options. Thanks for your input Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vivian891223 <@t> yahoo.com Mon Nov 30 09:39:06 2009 From: vivian891223 <@t> yahoo.com (Vivian Johnson) Date: Mon Nov 30 09:39:08 2009 Subject: [Histonet] (no subject) Message-ID: <229169.5366.qm@web35805.mail.mud.yahoo.com> Unsuscribe, please. From akbitting <@t> geisinger.edu Mon Nov 30 09:39:09 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Nov 30 09:39:19 2009 Subject: [Histonet] Autostainer service Message-ID: <4B13A0CE.2B7F.00C9.0@geisinger.edu> I need to pick your collective brains here about getting a service contract for my Dako Autostainers. I remember seeing a posting awhile back with the name of a company, I think from MD, that will service the Dako Autostainers now that Dako will no longer support them. If anyone has a suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From carrolpb <@t> umdnj.edu Mon Nov 30 09:58:57 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Nov 30 09:59:09 2009 Subject: [Histonet] (no subject) In-Reply-To: <229169.5366.qm@web35805.mail.mud.yahoo.com> References: <229169.5366.qm@web35805.mail.mud.yahoo.com> Message-ID: <4B13EBC1.9090208@umdnj.edu> > Unsuscribe, please. In the ever prescient words of Roger Moretz... "First of all, that's not even a word. Secondly, that's not the way it's done. I'm sure others will point you in the right direction..." ;) From paintedsplashes <@t> yahoo.com Mon Nov 30 12:08:57 2009 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Mon Nov 30 12:09:00 2009 Subject: [Histonet] FTE Workload Message-ID: <455776.60314.qm@web30704.mail.mud.yahoo.com> Can you please send me information on justifying FTE per workload.? Our facility has not increased in case number but the complexity and block# per case has increased.? It is hard to make a non-histology administrative person understand how you cannot compare workload in Pathology against workload in the Clinical lab....apples to oranges kind of thing.? Please advise. ? Jeanne Clark Mission Hospital Asheville, NC 28801 ? ? 600 Skyloft Dr. Unit 203 Asheville, NC 28801 423-612-1213 cell 828-213-5169 office ? ? From rjbuesa <@t> yahoo.com Mon Nov 30 12:17:40 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 30 12:17:43 2009 Subject: [Histonet] FTE Workload In-Reply-To: <455776.60314.qm@web30704.mail.mud.yahoo.com> Message-ID: <138556.23730.qm@web65714.mail.ac4.yahoo.com> Under separate cover I am sending you an article about staffing in histology. Ren? J. --- On Mon, 11/30/09, Jeanne Clark wrote: From: Jeanne Clark Subject: [Histonet] FTE Workload To: histonet@lists.utsouthwestern.edu Date: Monday, November 30, 2009, 1:08 PM Can you please send me information on justifying FTE per workload.? Our facility has not increased in case number but the complexity and block# per case has increased.? It is hard to make a non-histology administrative person understand how you cannot compare workload in Pathology against workload in the Clinical lab....apples to oranges kind of thing.? Please advise.. ? Jeanne Clark Mission Hospital Asheville, NC 28801 ? ? 600 Skyloft Dr. Unit 203 Asheville, NC 28801 423-612-1213 cell 828-213-5169 office ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nde789 <@t> googlemail.com Mon Nov 30 12:28:27 2009 From: nde789 <@t> googlemail.com (Nicholas Evans) Date: Mon Nov 30 12:28:31 2009 Subject: [histonet] Cleaning and re-using slides Message-ID: Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and labor-intensive - it is vital that the slides are spotlessly clean before re-treating. We (when I say we, i mean the student lackies who get drafted in the lab, not me, hoho) currently physically scrub them with various solvents, such as citrisolve, acetone etc. we use Fisherbrand Superfrost/plus slides. Anyone got any ideas how to speed this up/automate ? is there a machine available? And would anyone care to vote on the lunacy or soundness of this idea? (Please post to me, not the list.) We are not a clinical lab, by the way. Nick From carrolpb <@t> umdnj.edu Mon Nov 30 12:40:42 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Nov 30 12:40:53 2009 Subject: [histonet] Cleaning and re-using slides In-Reply-To: References: Message-ID: <4B1411AA.6020506@umdnj.edu> sorry... your boss is crazy! ;) Nicholas Evans wrote: > Dear all, > > In our lab our boss is adamant that we must clean and recycle old unmounted > microscope slides (which have paraffin and cryo sections on them). It is > driving us nuts, as the process of cleaning the slides is incredibly tedious > and labor-intensive - it is vital that the slides are spotlessly clean > before re-treating. We (when I say we, i mean the student lackies who get > drafted in the lab, not me, hoho) currently physically scrub them with > various solvents, such as citrisolve, acetone etc. we use Fisherbrand > Superfrost/plus slides. > > Anyone got any ideas how to speed this up/automate ? is there a machine > available? And would anyone care to vote on the lunacy or soundness of this > idea? (Please post to me, not the list.) We are not a clinical lab, by the > way. > > Nick > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From trathborne <@t> somerset-healthcare.com Mon Nov 30 13:22:07 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Nov 30 13:22:12 2009 Subject: [Histonet] Cap for Centra GP8R swinging bucket Message-ID: Hi everyone, I need your help. We have a Thermo IEC centrifuge which I need two bucket lids for. Catalog # 6377, but I have been told by the manufacturer that the item has been discontinued, and their stock supply of that part has been depleted. If there is anyone who may have this item please contact me offline. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From asmith <@t> mail.barry.edu Mon Nov 30 13:30:48 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Nov 30 13:31:58 2009 Subject: [histonet] Cleaning and re-using slides In-Reply-To: References: Message-ID: Cleaning slides strikes me as a very poor use of student time. If they are paid even 3/4 minimum wage, it is uneconomical. If they are unpaid, it is unfair to set them a task they can learn nothing from. -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas Evans Sent: Monday, November 30, 2009 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [histonet] Cleaning and re-using slides Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and labor-intensive - it is vital that the slides are spotlessly clean before re-treating. We (when I say we, i mean the student lackies who get drafted in the lab, not me, hoho) currently physically scrub them with various solvents, such as citrisolve, acetone etc. we use Fisherbrand Superfrost/plus slides. Anyone got any ideas how to speed this up/automate - is there a machine available? And would anyone care to vote on the lunacy or soundness of this idea? (Please post to me, not the list.) We are not a clinical lab, by the way. Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Nov 30 13:58:47 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Mon Nov 30 13:58:45 2009 Subject: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA137E79C24F@NADCWPMSGCMS03.hca.corpad.net> Has anyone discovered anything new since the last discussion in the archives for finding lymph nodes in colonic fat??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From DKBoyd <@t> chs.net Mon Nov 30 14:20:37 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Nov 30 14:20:03 2009 Subject: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA137E79C24F@NADCWPMSGCMS03.hca.corpad.net> Message-ID: We use Lymph Node Revealing Solution from Polyscientific. Works great for us. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Smith Wanda Sent by: histonet-bounces@lists.utsouthwestern.edu 11/30/2009 02:59 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? Has anyone discovered anything new since the last discussion in the archives for finding lymph nodes in colonic fat??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From gvdobbin <@t> ihis.org Mon Nov 30 14:23:56 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Nov 30 14:24:12 2009 Subject: [Histonet] Morgue topic: Chain of Custody for personal effects Message-ID: Hi Folks, Can any of you share with me your procedure for ensuring chain of custody for personal effects of decedents. Along with documenting a clear procedure for all stakeholders to abide by, I also need to solve some the practical aspects like what to use for a tamper-resistant bag for the effects and should I have a 3-part form (1 copy for us, 1 for the Commissionaire and 1 the funeral home) that lists all the personal items, money etc. Jewellery is only removed if there is a risk of damage (eg wrist watches, necklaces). Piercings are generally not removed. Rings are usually taped. A little background info: Most of the decedents we receive in our morgue are "at the back door", usually via ambulance (our coroner system does not have their own vehicles) or from the ER. Occasionally we will get a hospital case from the nursing floors. The Commissionaire (security) receives the bodies and unlocks the morgue for delivery. The pathologist on call is notified and an autopsy is scheduled. After the autopsy we inform the Commissionaire that the remains have been released and he/she in turn notifies the funeral home and looks after signing the remains over to the funeral home staff. Thank you! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From ttruscot <@t> vetmed.wsu.edu Mon Nov 30 14:36:32 2009 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Mon Nov 30 14:36:37 2009 Subject: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? In-Reply-To: References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA137E79C24F@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4BBA1A0EFBB@CVMMBX.vetmed.wsu.edu> Hi Wanda, Soaking tissues in white vinegar tends to "clear" the fat and make the lymph nodes more visible, but it might affect some tests. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Monday, November 30, 2009 12:21 PM To: Smith Wanda Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? We use Lymph Node Revealing Solution from Polyscientific. Works great for us. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Smith Wanda Sent by: histonet-bounces@lists.utsouthwestern.edu 11/30/2009 02:59 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? Has anyone discovered anything new since the last discussion in the archives for finding lymph nodes in colonic fat??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 30 15:01:03 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 30 15:01:08 2009 Subject: [histonet] Cleaning and re-using slides In-Reply-To: Message-ID: <299270.60679.qm@web65701.mail.ac4.yahoo.com> Place the slides in a hot (90?C) of a 2%dishwasherer soap solution. Repeat 3 times and the slides will be cleaned. The only problem that you will have is that the slides can stick to one another. You may want to place the used slides in racks (the same used to stain), so they will be separated from one another. In this way you will only need to wash them with clean water. You will see! Ren? J. --- On Mon, 11/30/09, Nicholas Evans wrote: From: Nicholas Evans Subject: [histonet] Cleaning and re-using slides To: histonet@lists.utsouthwestern.edu Date: Monday, November 30, 2009, 1:28 PM Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and labor-intensive - it is vital that the slides are spotlessly clean before re-treating. We (when I say we, i mean the student lackies who get drafted in the lab, not me, hoho) currently physically scrub them with various solvents, such as citrisolve, acetone etc. we use Fisherbrand Superfrost/plus slides. Anyone got any ideas how to speed this up/automate ? is there a machine available? And would anyone care to vote on the lunacy or soundness of this idea? (Please post to me, not the list.) We are not a clinical lab, by the way. Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 30 15:02:50 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 30 15:02:55 2009 Subject: [histonet] Cleaning and re-using slides In-Reply-To: Message-ID: <605545.60062.qm@web65709.mail.ac4.yahoo.com> Except for learning how to clean slides and how unfair life can be even in that environment! Ren? J. --- On Mon, 11/30/09, Smith, Allen wrote: From: Smith, Allen Subject: RE: [histonet] Cleaning and re-using slides To: "'Nicholas Evans'" Cc: "'Histonet@lists.utsouthwestern.edu'" Date: Monday, November 30, 2009, 2:30 PM Cleaning slides strikes me as a very poor use of student time.? If they are paid even 3/4 minimum wage, it is uneconomical.? If they are unpaid, it is unfair to set them a task they can learn nothing from. -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas Evans Sent: Monday, November 30, 2009 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [histonet] Cleaning and re-using slides Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and labor-intensive - it is vital that the slides are spotlessly clean before re-treating. We (when I say we, i mean the student lackies who get drafted in the lab, not me, hoho) currently physically scrub them with various solvents, such as citrisolve, acetone etc. we use Fisherbrand Superfrost/plus slides. Anyone got any ideas how to speed this up/automate - is there a machine available? And would anyone care to vote on the lunacy or soundness of this idea? (Please post to me, not the list.) We are not a clinical lab, by the way. Nick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cliffberger <@t> mac.com Mon Nov 30 16:05:58 2009 From: cliffberger <@t> mac.com (Cliff Berger) Date: Mon Nov 30 16:06:31 2009 Subject: [Histonet] Anything New for Finding Lymph Nodes in Colon Fat? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA137E79C24F@NADCWPMSGCMS03.hca.corpad.net> Message-ID: Dissect Aid. Just Google it. -- Best regards! Cliff Berger Decal Chemical Corp 1-800-428-5856 On 11/30/09 2:58 PM, "Smith Wanda" wrote: > Has anyone discovered anything new since the last discussion in the archives > for finding lymph nodes in colonic fat??? > Thanks, Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error and > that any use, dissemination, distribution, forwarding, printing, or copying of > this email or any attached files is strictly prohibited. If you have received > this email in error, please immediately purge it and all attachments and > notify the sender by reply email or contact the sender at the number listed. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Nov 30 16:59:06 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Nov 30 17:00:26 2009 Subject: [Histonet] Morgue topic: Chain of Custody for personal effects In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8359F@EMAIL.archildrens.org> I wouldn't call it a chain of custody but we do log in personal effects when a body is delivered to the morgue. It is logged in and out on the morgue log. We also document the funeral home, pick up service, etc.. took the personal belongings with them with the body. The funeral home signs the log as well as pathology or nursing staff. (whoever releases the body for the hospital) Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Monday, November 30, 2009 2:24 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Morgue topic: Chain of Custody for personal effects Hi Folks, Can any of you share with me your procedure for ensuring chain of custody for personal effects of decedents. Along with documenting a clear procedure for all stakeholders to abide by, I also need to solve some the practical aspects like what to use for a tamper-resistant bag for the effects and should I have a 3-part form (1 copy for us, 1 for the Commissionaire and 1 the funeral home) that lists all the personal items, money etc. Jewellery is only removed if there is a risk of damage (eg wrist watches, necklaces). Piercings are generally not removed. Rings are usually taped. A little background info: Most of the decedents we receive in our morgue are "at the back door", usually via ambulance (our coroner system does not have their own vehicles) or from the ER. Occasionally we will get a hospital case from the nursing floors. The Commissionaire (security) receives the bodies and unlocks the morgue for delivery. The pathologist on call is notified and an autopsy is scheduled. After the autopsy we inform the Commissionaire that the remains have been released and he/she in turn notifies the funeral home and looks after signing the remains over to the funeral home staff. Thank you! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From nde789 <@t> googlemail.com Mon Nov 30 19:38:06 2009 From: nde789 <@t> googlemail.com (Nicholas Evans) Date: Mon Nov 30 19:38:15 2009 Subject: [histonet] Cleaning and re-using slides In-Reply-To: References: Message-ID: Thanks very much everyone for the responses!!! Pretty much everyone thinks this is a ridiculous idea (to put it politely). Plus I found out that we may be overpaying for our slides - we buy Superfrost from Fisher at $350/10 gross, whereas Gorilla Scientific are selling the same type of slide for about a third of this cost (trial pending). It's still not totally clear to me whether used slides (we are an academic lab working on transgenic mice, not a clinical lab) can be sent for glass recycling, but they probably end up in landfill/incinerators (they most likely are classed as contaminated sharps waste). Best wishes Nick 2009/11/30 Nicholas Evans > Dear all, > > In our lab our boss is adamant that we must clean and recycle old > unmounted microscope slides (which have paraffin and cryo sections on them). > It is driving us nuts, as the process of cleaning the slides is incredibly > tedious and labor-intensive - it is vital that the slides are spotlessly > clean before re-treating. We (when I say we, i mean the student lackies who > get drafted in the lab, not me, hoho) currently physically scrub them with > various solvents, such as citrisolve, acetone etc. we use Fisherbrand > Superfrost/plus slides. > > Anyone got any ideas how to speed this up/automate ? is there a machine > available? And would anyone care to vote on the lunacy or soundness of > this idea? (Please post to me, not the list.) We are not a clinical lab, > by the way. > > Nick >