[Histonet] CD11c for mouse tissue
Melissa Mazan
melissa.mazan <@t> tufts.edu
Fri May 29 13:18:31 CDT 2009
Hi all - I'm looking for a good antibody to use on mouse tissue (frozen)
to look for CD11c - does anyone have a suggestion for a good antibody
source? Thanks in advance - Melissa
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> Today's Topics:
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> 1. RE: Sakura film coverslipper xylene residue issue (Jason McGough)
> 2. Looking for travel job in Ca or Florida (azhistotech <@t> cox.net)
> 3. MAP-2 antibody (Lott, Robert)
> 4. Re: Microtome Problems (mari.ann.mailhiot <@t> leica-microsystems.com)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 29 May 2009 08:56:51 -0600
> From: Jason McGough <jmcgough <@t> clinlab.com>
> Subject: RE: [Histonet] Sakura film coverslipper xylene residue issue
> To: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
> Cc: Histonet <@t> lists.utsouthwestern.edu, Karin Groeger
> <KGroeger <@t> USLABS.net>, histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID: <20090529085651.6pa1aldhvb44sw0s <@t> mail.clinlab.com>
> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes";
> format="flowed"
>
> It has nothing to do with your coverslipper. Ours is from the mid 90's
> and we have had the brown spots a couple of times. We found that there
> to be water in our xylene. The little water droplets on the side of a
> container will cause this. Make sure everything that holds xylene is
> completely dry. Once we did this, we had no more brown spots. Good luck!
>
> As far as xylene residue, it is your dispensing volume on the
> coverslipper that is causing this. Try turning the flow down and see
> what happens.
>
> Jason McGough HT(ASCP)
> Account Representative-Anatomic Pathology
> Clinical Laboratory of the Black Hills
> 2805 5th Street Suite 210
> Rapid City, SD 57701
> 605-343-2267
> jmcgough <@t> clinlab.com
>
>
> Quoting Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>:
>
>
>> I have an older Sakura coverslipper, circa 1997ish. We have noticed an
>> increasing number of brown spots on tissue sections recently. This does
>> not occur if the same slides are covered with glass coverslips. It does
>> not look like a drying artefact, occurs randomly throughout tissue types,
>> location on tissue, i.e., edge, center. I've even showed these spots to
>> the rep who admitted she has never seen this artefact before. We use
>> only the Tissue Tek tape, we maintain the equipment on a regular basis.
>> The xylene flow is good. Our only problem are these random brown spots.
>> Any suggestions?
>> Jackie O'
>>
>>
>>
>> "Karin Groeger" <KGroeger <@t> USLABS.net>
>> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
>> 05/29/2009 08:19 AM
>>
>> To
>> "Scott" <lscott <@t> sfcn.org>, <Histonet <@t> lists.utsouthwestern.edu>
>> cc
>>
>> Subject
>> RE: [Histonet] Sakura film coverslipper xylene residue issue
>>
>>
>>
>>
>>
>>
>> Hi Scott,
>> We place ours in front of a fan to dry if we are in a hurry, even if
>> we air dry them we do not have a problem with the xylene residue,
>> perhaps you have a bad batch, check your lot # and call Sakura. We
>> coverslip over 1,000 slides a day and have not seen this.
>>
>> Karin Groeger
>>
>> Histology Supervisor
>>
>> US LABS, Irvine,CA
>>
>> 949-450-0145 ext. 649
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
>> Sent: Thursday, May 28, 2009 10:03 PM
>> To: Histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Sakura film coverslipper xylene residue issue
>>
>> Hi,
>> We have a newer Sakura film coverslipper, does anyone have
>> experience with this piece of equipment. I have a problem with the
>> slides having a light xylene residue, after the slides come off of the
>> coverslipper.
>>
>> How do you dry them? If I leave the slides on the coverslipper to air
>> dry the xylene residue is noticeable. If I lightly wipe off all of the
>> slides as they come off the coverslipper they look clean.
>>
>> How do you guys do it? I think it is a waste of time to wipe off all of
>> the slides. I don't think the xylene flow is too high.
>>
>> Thanks for your help,
>>
>> Scott Hendricksen HT(ASCP)
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
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>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 29 May 2009 8:48:16 -0700
> From: <azhistotech <@t> cox.net>
> Subject: [Histonet] Looking for travel job in Ca or Florida
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20090529114816.2H1BE.137513.imail <@t> fed1rmwml44>
> Content-Type: text/plain; charset=utf-8
>
> Hi Netters,
> Is anyone looking for a Histologist in California or Florida? I am an HT, (ascp) with Florida license, 18 years experience, 5 years management and know all aspects of Histology. Looking for a Travel position or short term assignment. Please respond to this email and I can forward you my resume. Thank you..
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 29 May 2009 11:35:16 -0500
> From: "Lott, Robert" <Robert.Lott <@t> trinitymedicalonline.com>
> Subject: [Histonet] MAP-2 antibody
> To: <histonet <@t> lists.utsouthwestern.edu>
> Cc: Christa Hladik <Christa.Hladik <@t> UTSouthwestern.edu>
> Message-ID:
> <4A3619571D9F6C4CB79C980E91DBE4E60106801E <@t> TNTRIEXEVS03.triadhospitals.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Everyone,
>
> One of our pathologists is interested in MAP-2 IHC for the following
> application:
>
>
>
> Microtubule Associated Protein-2 Is a Sensitive Marker of Primary and
> Metastatic Neuroblastoma.
>
> Krishnan C, Heerema-McKenney A, Arber DA. Department of Pathology,
> Stanford University, Stanford, CA.
>
>
>
> Comment: Microtubule associated protein-2 (MAP-2) is a protein expressed
> in high levels in cells derived from the neural crest.
>
> It is a very robust marker of neuronal differentiation and has been used
> most often in evaluating neuronal differentiation in tumors
>
> of the central nervous system. MAP-2 showed comparable sensitivity in
> staining primary neuroblastomas
>
> (pre- and post-treatment samples) as compared to synaptophysin,
> chromogranin, CD56, and beta-catenin.
>
> In contrast to other markers of neuroblastoma, MAP-2 did not show
> significant cross reactivity to native bone marrow
>
> precursors, thus eliminating a potential source of confusion.
>
>
>
> Does anyone use this antibody for this or like applications....
>
> I have looked and am "fairly" sure that there is not an IVD available,
> but would still be interested in knowing which antibody you use.
>
>
>
> Thanks,
>
>
>
> Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology
>
> Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL
> 35213/ 205-592-5387
>
>
>
>
>
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> ------------------------------
>
> Message: 4
> Date: Fri, 29 May 2009 11:50:19 -0500
> From: mari.ann.mailhiot <@t> leica-microsystems.com
> Subject: Re: [Histonet] Microtome Problems
> To: "Veterinary Services Laboratory" <vetlab <@t> caribsurf.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF0DF77AAF.64653700-ON862575C5.005B3E19-862575C5.005C805F <@t> leica-microsystems.com>
>
> Content-Type: text/plain; charset=US-ASCII
>
> HI Sharon
>
> I am going to refer your email to my collegues in Germany. They will be
> able to get you the help you need and all the information..
>
> Best Regards
>
> Mari Ann Mailhiot BA HT ASCP
> Application Specialist/Trainer
> Leica Microsystems
> Biosystems Division
> Technical Assistance Center
> 800 248 0123 x7267
> 847 236 3063 fax
> mari.ann.mailhiot <@t> leica-microsystems.com
> www.leica-microsystems.com
>
>
>
> "Veterinary
> Services
> Laboratory" To
> <vetlab <@t> caribsurf <histonet <@t> lists.utsouthwestern.edu>
> .com> cc
> Sent by:
> histonet-bounces@ Subject
> lists.utsouthwest [Histonet] Microtome Problems
> ern.edu
>
>
> 05/29/2009 09:11
> AM
>
>
>
>
>
>
> Hello All
>
>
>
> I'm new to the group and histo. (3 years) so far I'm getting a lot of
> useful
> tips from all the messages. Thank you.
>
>
>
> I have two microtomes that were working almost perfectly before I had a
> technician service them. Now, the Leica 2035 Jung biocuts' blade holder is
> twisting to the side (going up at one end). I have tried tightening,
> slacking, and cleaning the entire assembly to no avail. Does anyone know
> of
> where I might be able to source one or even how to fix the one I have.
>
>
>
> Secondly, the Leitz 1512 rotary microtome, is now jumping forward and
> giving
> a loud clanking noise. It takes huge chunks out of the tissue.
>
>
>
> Any and all help is appreciated,
>
>
>
> Thank you
>
> Sharon
>
>
>
> Ms. Sharon Drayton
>
> Veterinary Services Laboratory
>
> Ministry of Agriculture & Rural Development
>
> The Pine
>
> St. Michael BB11091
>
> Barbados
>
>
>
> Tel: (246) 427-5492 or (246) 427-5073
>
> Fax: (246)426-7517
>
> Email: vetlab <@t> caribsurf.com
>
> Website: www.agriculture.gov.bb
>
>
>
>
>
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> End of Histonet Digest, Vol 66, Issue 35
> ****************************************
>
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel: 508-839-5395
fax: 508-839-7903
email: melissa.mazan <@t> tufts.edu
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