Re: Re: [Histonet] Chemicals that inactivate the primary antibody

[TF]

Hi, but I dont want to destory everything....
I am trying to see if I can prevent the binding of another 2nd antibody to the primary antibody for the first antigen!
There fore you can perform double staining from same sepecies-derived primary antibodies!


2009-05-17 



TF 



发件人： Rene J Buesa 
发送时间： 2009-05-17  00:54:26 
收件人： Histonet <@t> lists.utsouthwestern.edu; tifei 
抄送： 
主题： Re: [Histonet] Chemicals that inactivate the primary antibody 
 
Try bleach and you will destroy everything!
René J.

--- On Sat, 5/16/09, TF <tifei <@t> foxmail.com> wrote:


From: TF <tifei <@t> foxmail.com>
Subject: [Histonet] Chemicals that inactivate the primary antibody
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, May 16, 2009, 12:48 PM


Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen?
I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally.

2009-05-17 



TF 
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