[Histonet] 4% PFA/10% formalin in PB or in PBS?

[TF]

Hi, I want to ask this questions because I read different recipes in preparaing fixative solution...

Here we perfuse animals with 0.9% saline, then 4% PFA in 0.1M PB...that is not a physiological solution...
I see other people using 4% PFA in PBS.... will this lead to different brain shrinkage?

Thanks.

2009-05-05 



TF