[Histonet] xylene

[Rene J Buesa]

I also used recycled xylene before I switched to mineral oil and I used a B/R Corp recycler and never had that problem.
The temperature sensor of your recycler probably failed and started to collect mixed xylene. Try to check the sensor.
René J.

--- On Tue, 4/28/09, Moffatt, Loretta <lmoffatt <@t> susquehannahealth.org> wrote:

From: Moffatt, Loretta <lmoffatt <@t> susquehannahealth.org>
Subject: [Histonet] xylene
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, April 28, 2009, 2:06 PM

We have recently had a problem with poor tissue processing (soft to cut/ poor
staining).  None of the tissue was processed the first time, and ultimately,
some fragile tissue did not survive.  The alcohols checked out and the
processor, itself, does not appear to be an issue.  We used recycled xylene in
the processor first, followed by fresh xylene.   The xylene, which came from a
CBG recycler, failed the test for contamination, after the fact.  Has anyone
seen this kind of problem?  We would appreciate any input.   Thank you.



Loretta Moffatt, MT(ASCP),MHA
Laboratory Manager
777 Rural Avenue
Williamsport, PA 17701
570-321-2326 (F)570-321-2489 lmoffatt <@t> susquehannahealth.org

Patience is never more important than when you are on the verge of losing it.

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