[Histonet] Re-processing cartilage

[V. Neubert]

Hello Histonetters,

I have processed some strips of cartilage (about 2cm long, 3mm of height, 2mm wide) with my standard protocol.

I cannot section them. They just start to break away as soon as they hit the blade. I tried to section them really cold and also at RT, but that did not have any effect.

I guess sth went wrong when processing (other kinds of tissue were fine).

My protocol:
Reagent		Time [h]	Spin (Microm ST120)

NBF		1		1
70% Isopropanol	1		1
80% Isopropanol	1		1
95% Isopropanol	1		1
abs Isopropanol	1		1
abs Isopropanol	1		1
abs Isopropanol	1		1
Xylene		1		1
Xylene		1		1
Xylene		1		1
Paraffin  I	1		1
Paraffin II	1		1

Maybe someone can help me out and give some hints.

Thanks a lot!

V. Neubert