AW: [Histonet] C4d Antibody

[Andi Kappeler]

Hi César

we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for
cryosections of kidney biopsies (indirect immunofluorescence), it won't work
on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d
(BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH buffer
(Tris-EDTA, pH 9.0; Tris-Urea, pH 9.5). Hope this helps.

Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Cesar
Francisco Romero
Gesendet: Dienstag, 24. März 2009 05:01
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] C4d Antibody


Dear People from Histonet

 

Could you share your experience with C4d Antibody ?

 

I couldn't get any results with the mouse monoclonal from Quidel.

 

I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe.

 

I work in a Public Hospital with many kidney transplantations per year.

 

Thank You in advance.

 

César Romero

Buenos Aires

Argentina

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