From azhisto <@t> hotmail.com Sun Mar 1 10:33:40 2009 From: azhisto <@t> hotmail.com (azhisto Read) Date: Sun Mar 1 10:33:46 2009 Subject: [Histonet] job Message-ID: New anatomic pathology lab opening in Phoenix, AZ., to submit a resume please sent to twincrest@buckeye-express.com, attention Bernie. _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail?. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_WL_HM_express_032009#colortheme From azhisto <@t> hotmail.com Sun Mar 1 11:02:52 2009 From: azhisto <@t> hotmail.com (azhisto Read) Date: Sun Mar 1 11:02:56 2009 Subject: [Histonet] New Anatomic Pathology Lab Message-ID: Anyone interested should submit a resume to Bernie at twincrest@be.org, or twincrest@buckeye-express.com Thanks, Bernie _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From azhisto <@t> hotmail.com Sun Mar 1 12:52:24 2009 From: azhisto <@t> hotmail.com (azhisto Read) Date: Sun Mar 1 12:52:29 2009 Subject: [Histonet] New Anatomic Pathology Lab Message-ID: This new lab will be opening soon, please DO NOT respond to this email, for inquires please send emails to ATTN: Bernie at twincrest@bex.net or twincrest@buckeye.com, thank you. _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From azhisto <@t> hotmail.com Sun Mar 1 13:17:03 2009 From: azhisto <@t> hotmail.com (azhisto Histology) Date: Sun Mar 1 13:17:08 2009 Subject: [Histonet] New Anatomic Pathology Lab Message-ID: Please DO NOT RESOND to this email, please send your inquires to ATTN:Bernie at twincrest@bex.net, thank you, I have nothing to do with the hiring process, this is a 40,000 gastric biopsy lab, he is looking for 4 histologists and a Histology supervisor,the lab will be located close to Banner Good Sam. Please contact Bernie at twincrest@bex.net. _________________________________________________________________ Windows Live? Contacts: Organize your contact list. http://windowslive.com/connect/post/marcusatmicrosoft.spaces.live.com-Blog-cns!503D1D86EBB2B53C!2285.entry?ocid=TXT_TAGLM_WL_UGC_Contacts_032009 From AnthonyH <@t> chw.edu.au Sun Mar 1 16:10:10 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Mar 1 16:10:31 2009 Subject: [Histonet] sodium 5.5-diehylbarbiturate In-Reply-To: <001a01c998e3$95b03100$b364a8c0@LazoAnatomija> Message-ID: Lazo, This is our technique: Adenosine Triphosphatase (ATPases) Use Demonstration of muscle fibre types. Underlying Principle The principle relies on the ability of the enzyme to remove the terminal phosphate from the ATP, which then combines with calcium in the incubation solution to form an insoluble calcium phosphate. Cobalt is then exchanged for the calcium, which, after reaction with ammonium sulphide, forms a black, insoluble cobalt sulphide at the site of enzyme activity. Fixation and Sectioning Air dried unfixed 8?m cryostat sections Reagents 1. Acid Pre-incubation medium 0.2M Sodium Acetate Sodium Acetate Anhydrous 0.82 g Distilled water 50 ml 0.2M Acetic Acid Glacial Acetic Acid 0.6 ml Distilled water 50 ml 0.2M Acetate Buffer: pH 4.3 pH 4.6 0.2M Sodium Acetate 11 ml 18 ml 0.2M Acetic Acid 12 ml 13 ml Adjust to pH 4.3 or 4.6 with 0.2M Sodium Acetate or 0.2M Acetic Acid 2. 7.5% Calcium Chloride Calcium Chloride 7.5g Distilled water 100ml 3. 7.05% Glycine Glycine 7.05g Distilled water 100ml Aliquot 2ml into tubes labeled "G" and store at -20oC 4. 5.625% Sodium Chloride Sodium Chloride 5.625g Distilled water 100ml 5. 3.5% Sodim Hydroxide Sodium Hydroxide 3.5g Distilled water 100ml 6. Alkaline Stock Warning: Irritant - see MSDS 7.05% Glycine 2ml 7.5% Calcium Chloride 2ml 5.625% Sodium Chloride 2ml 3.5% Sodium Hydroxide 2ml Distilled water 42ml 7. Incubating Medium 1. Prepare fresh Alkaline Stock for each run 2. pH close to 11 and place in 37oC Oven for 20minutes 3. pH to 11 using 0.1M NaOH (to activate the ATP) 4. Add 0.04g ATP (Sigma A-7699) 5. pH to 9.35 using 0.1M HCl 8. 1% calcium chloride Calcium chloride 5.0 g Distilled water 500ml 9. 2% Cobalt chloride Warning: Suspected Carcinogen - see MSDS Cobalt chloride 10.0 g Distilled water 500 ml 10. 1% Ammonium sulphide Warning: Flammable liquid, Irritant, Toxic stench - see MSDS 20% ammonium sulphide 0.5 ml Distilled water 9.5 ml Staining Method 1. Place one slide in 4.3 and one in 4.6 buffer at room temperature for 20 minutes 2. Wash each in distilled water 3 times 3. Place these slides (one from the 4.3 solution , one from 4.6 solution and the 9.4 slide) in the 9.4 substrate at 37?C for pH 9.4 10 mins pH 4.6 30 mins pH 4.3 45 mins 4. Place slides in 2 changes of 1% calcium chloride 3 min each 5. Place slides in 2% cobalt chloride 3 min 6. Wash well in distilled water 7. In fume cupboard drain slides well and place in 1% ammonium sulphide solution for 1 min 8. Wash well in tap water 9. Dehydrate clear and mount. Results pH 9.4 Type 1 fibres pale Type 2A fibres intermediate Type 2B fibres dark pH 4.3 and pH 4.6 pH 4.3 pH 4.6 Type 1 fibres dark dark Type 2A fibres pale pale Type 2B fibres pale intermediate Type 2C fibres intermediate dark Notes This is a complicated stain and there are several areas in which one needs to be careful in order to achieve a good fibre type differentiation. 1. The pH of all solutions is critical 2. Timing is crucial 1. The stock ammonium sulfide must still be yellow. As it ages or oxidizes, it becomes more red and cannot be used. 2. All solutions must be adjusted at the temperature they will be used. Difficulties with ATPase technique (1) Problem Possible Causes Remedies Poor fibre type differentiation 1. Inaccurate pH reading. 2. Severe pathological condition. 1. Carefully calibrate pH meter using standard solutions of known pH. 2. Assess fibre typing using pH 4.3/4.6 ATPase sections Very pale ATPase reaction 1. Incubation time too short 2. pH of incubating medium too low 3. Incubation temperature < 37oC 4. Ammonium sulfide solution has turned deep yellow or brown due to oxidation. 1. Incubate sections longer 2. Carefully calibrate pH meter 3. Check temperature of incubator 4. Ensure cap on ammonium sulfide solution is replaced tightly after use Very dark ATPase reaction 1. Over incubation 2. pH too high 1. Reduce incubation time 2. Carefully calibrate pH meter Artefact patches within muscle fibres 1. Dirt or grease on glass slides 2. Fingerprint on slide under sections 1. Ensure clean slides are used 2. Pick up glass slides at the opposite end to where the section is to be positioned References 1. Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lazo Pendovski Sent: Saturday, 28 February 2009 12:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sodium 5.5-diehylbarbiturate Hi, I need help with ATPase muscle fiber staining in lambs. I am investigating the skeletal muscle fibers in lambs breaded extensively in mount-hills regions in Macedonia. But, I have a problem with a one chemical - sodium 5.5-diehylbarbiturate. The problem is that I can't get this substance in my country. So please, does any one know substitute or a protocol without Sodium barbital? Any help you can give me would be greatly appreciated. Lazo ass. m-r Lazo Pendovski, M.Sc., DVM University of St. "Cyril and Methodist" Faculty of Veterinary medicine Department of Functional Morphology Lazar Pop- Trajkov 5-7 1000 Skopje, R. of Macedonia tel: +389 2 3240 710 fax: +389 2 3114 619 mob:+389 70 766 017 e-mail: lpendovski@fvm.ukim.edu.mk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Mar 1 16:18:14 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Mar 1 16:18:26 2009 Subject: [Histonet] Quality assurance program In-Reply-To: <867120.97110.qm@web65708.mail.ac4.yahoo.com> Message-ID: Look at the processes. Human error is unavoidable. Rarely will people deliberately make a mistake. Remember that it is probably the process that is at fault. Try to identify where the critical processes are, where mistakes are likely to happen and implement methods to minimise them. Also remember that it not the individual that fails but the team, there fore the team finds the error and corrects it before it leaves its influence. A great saying: "A Champion team will always defeat a team of champions" Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, 28 February 2009 5:01 AM To: histonet Subject: [Histonet] Quality assurance program I would like to do something different (more) than what we are doing right now with qa/qc.? We write everything down that we do so we can track it and then when there are mistakes I will talk to the person who makes them.? What are people doing in their labs to stop these mistakes in the first place? I know there are tools out there like L.E.A.N, but I was hoping to go another route for now.? I have added more check lists and made many process changes but sometimes I feel as though people need extra incentive to do well.? Sad but true.? Thanks for any help!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Mar 1 16:20:42 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Mar 1 16:20:59 2009 Subject: [Histonet] Sulfated Alcian Blue In-Reply-To: <116774.23931.qm@web83908.mail.sp1.yahoo.com> Message-ID: This is our technique: Sulphated Alcian Blue for Amyloid Amyloid has an increased avidity for Alcian blue in the presence of added salt. By fixing with an alkaline solution, SAB can be followed by various trichromic counterstains (Lendrum et al 1972 J Clin Pathol 25: 373-396). Fixation: 10% buffered formalin Microtomy: 7?m paraffin sections Solutions: 1. Acetic/Alcohol Rinse: 95% ethanol 45ml Distilled water 45ml Glacial Acetic Acid 10ml 2. Stock Solutions: a) 1% Alcian Blue (CI 74240) in 95% ethanol. Caution Flammable - see MSDS b) 1% Sodium Sulphate Hydrate in distilled water. 3. SAB Working Solution - prepare fresh: Stock solution (a) 18ml Stock solution (b) 18ml Glacial Acetic Acid 4ml Mix, stand 30 minutes before use. 4. 80% ethanol saturated with borax. Caution Flammable - see MSDS 5. Van Gieson counterstain. Staining Procedure: 1. Dewax, hydrate sections and wash for 10 minutes. 2. Rinse in acetic/alcohol, 2 minutes. 3. Place in SAB, 2 hours. 4. Rinse in acetic/alcohol, 2 minutes. 5. Wash in water. 6. Alkalinise in borax/alcohol, 30 minutes. 7. Wash in water. 8. Counterstain in Van Giesons. 9. Blot dry, dehydrate, clear and mount. Results: Amyloid Blue-green Collagen Red Muscle, cytoplasm Yellow Mast cell granules and some colloids Blue-green Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathanial nauss Sent: Saturday, 28 February 2009 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sulfated Alcian Blue I need some help, has any one used the sulfated alcian blue to stain for amyloid. We have a case that looks like it should be positive but it is not staining with the Congo Red. Any help would be great. Nathaniel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gorhamk <@t> verizon.net Sun Mar 1 19:15:47 2009 From: gorhamk <@t> verizon.net (Kathy Gorham) Date: Sun Mar 1 19:15:56 2009 Subject: [Histonet] log book Message-ID: <27E50DF4F8434674B769EE3C1A469907@kathy83b707eca> Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. From b-frederick <@t> northwestern.edu Mon Mar 2 07:30:42 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Mar 2 07:30:57 2009 Subject: [Histonet] Tube station In-Reply-To: Message-ID: <57B375F153B5417C981FFBF608D1C7AC@lurie.northwestern.edu> I stayed near Russell Square station but liked Victoria despite the hustle and bustle ....... Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, February 27, 2009 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station I always preferred King's Cross myself, but I was only visiting... Claire PS, Don't confuse us poor Americans. We're used to the 'L', etc. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 2/26/2009 3:32 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station There is only one tube station, Mornington Crescent!!. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Mar 2 08:10:08 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Mar 2 08:10:16 2009 Subject: [Histonet] log book In-Reply-To: <27E50DF4F8434674B769EE3C1A469907@kathy83b707eca> References: <27E50DF4F8434674B769EE3C1A469907@kathy83b707eca> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BCF3@LRGHEXVS1.practice.lrgh.org> The OR delivery all the specimens to us. We do not go to the OR at all for specimens. Whom ever brings the OR specimens must lay out all specimens with the slips. Any case that is missing a specimen, we reject the case and the OR runner takes it back to the people that work that case to figure out what they did wrong. If we accept a case, we time/date stamp as we sign the OR book. If it is after hours the OR delivers the specimens to the clinical specimen processing area and they sign for the specimens, then they take them to the Histology lab. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy Gorham [gorhamk@verizon.net] Sent: Sunday, March 01, 2009 8:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Cathy.Crumpton <@t> tuality.org Mon Mar 2 12:03:47 2009 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Mon Mar 2 12:03:53 2009 Subject: [Histonet] Cathy Crumpton is out of the office. Message-ID: I will be out of the office starting Mon 03/02/2009 and will not return until Thu 08/27/2009. If you have any histology concerns please route them to Connie Basinski during my absence. From tbraud <@t> holyredeemer.com Mon Mar 2 12:28:22 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Mar 2 12:28:29 2009 Subject: [Histonet] RE: specimen tracking from the OR In-Reply-To: <3a964b890003a38c@HolyRedeemer.com> Message-ID: We are a small hospital lab, too. There is a log book in the OR, where specimens are placed for Histo pick up. The circulating nurse puts a Patient sticker and handwrites all specimens removed from the patient, regardless of what they are, and whether or not they have been walked down, tubed, or waiting pickup. We make sure that all specimens we remove are listed in the book, then sign/date/time for them. Any specimen left for us and not in the book, or vice versa, we bring to the attention of the OR control desk. If we make a run and there are no specimens to pick up, then we still sign and date/time the log book as "no specimens". Any specimens brought to the department are checked before accepting. Crude and time consuming, yes....accurate, too. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 6. log book (Kathy Gorham) Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: "Kathy Gorham" Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Norm.Burnham <@t> propath.com Mon Mar 2 12:36:45 2009 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Mon Mar 2 12:37:59 2009 Subject: [Histonet] RE: specimen tracking from the OR In-Reply-To: References: <3a964b890003a38c@HolyRedeemer.com> Message-ID: <82C7248978CB50469FD6BA68EBBEFE67011DA6E5@exchange.propathlab.com> CAP provides good direction on this most critical laboratory issue: GEN.40530 Phase I N/A YES NO For specimens submitted to the laboratory from remote sites, is there a documented tracking system to ensure that all specimens are actually received? NOTE: Documentation should include time of dispatch and receipt, as well as condition of specimens upon receipt. An example of an acceptable tracking system is submission of a packing list (prepared by the client or courier) with each batch of client specimens, which may be checked against the specimens received by the laboratory. Some laboratory tests (e.g., coagulation assays) have limitations on time and temperature conditions between collection and analysis. This question applies to couriers/transportation systems that are part of the laboratory organization, not to outside courier systems. ____________________________________ Norm Burnham, MBA, MT(ASCP), CLS Director of Laboratory Operations ProPath - The Leader in Pathology Services 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell Email: norm.burnham@propath.com To learn more about ProPath, please visit http://www.ProPath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Monday, March 02, 2009 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specimen tracking from the OR We are a small hospital lab, too. There is a log book in the OR, where specimens are placed for Histo pick up. The circulating nurse puts a Patient sticker and handwrites all specimens removed from the patient, regardless of what they are, and whether or not they have been walked down, tubed, or waiting pickup. We make sure that all specimens we remove are listed in the book, then sign/date/time for them. Any specimen left for us and not in the book, or vice versa, we bring to the attention of the OR control desk. If we make a run and there are no specimens to pick up, then we still sign and date/time the log book as "no specimens". Any specimens brought to the department are checked before accepting. Crude and time consuming, yes....accurate, too. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 6. log book (Kathy Gorham) Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: "Kathy Gorham" Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. ----------------------------------------------------------------------------- ---- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Mon Mar 2 12:37:52 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Mar 2 12:37:59 2009 Subject: [Histonet] specimens Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635DFA@hpes1.HealthPartners.int> The first problem was with the tech who removed thespecimens from the OR without checking to make certain everything was included. She/he should never have left the OR without responding to the issue and that would have taken the blame off of the histotechs. We have OR bring specimens to us and sign in with a label as used on the container, initial and date/time everything. The techs accepting specimens have to sign off and accept responsibility at that point. When we did go to the OR, a few yaers ago, we had the same logbook in place there. The OR personnel had to sign everything in and when we picked the tissue up, we checked each specimen off in the logbook with our initials and time/date. Same process, only in reverse. Once anyone initialed the logbook, it was that person's responsibility as to the specimen integrity. Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From casperhempel <@t> gmail.com Mon Mar 2 12:52:30 2009 From: casperhempel <@t> gmail.com (Casper Hempel) Date: Mon Mar 2 12:52:35 2009 Subject: [Histonet] HIER Message-ID: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper From jclark <@t> pcnm.com Mon Mar 2 13:00:46 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Mar 2 13:00:50 2009 Subject: [Histonet] RE: log book Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39CF66E09@mail.pcnm.com> -----Original Message----- -----Original Message----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi Kathy, we are a small private lab who has the contract with the local hospital. What we have is a log book in the O.R. where the nurses have to enter the specimens for pick up; our courier than signs off on the log in the O.R. that he/she has picked up the specimen. Each specimen is checked against the log before being transported to the lab. That way, if a specimen is missing, it can be taken up with the O.R. staff immediately. Hope that is helpful to you. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: "Kathy Gorham" Subject: [Histonet] log book To: Message-ID: <27E50DF4F8434674B769EE3C1A469907@kathy83b707eca> Content-Type: text/plain; charset="iso-8859-1" Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. ------------------------------ From arvidsonkristen <@t> yahoo.com Mon Mar 2 13:10:40 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Mar 2 13:10:43 2009 Subject: [Histonet] Distilled water in the baths? Message-ID: <234672.67372.qm@web65711.mail.ac4.yahoo.com> Are people using tap water in their waterbaths? Has anyone noticed any problems with this? Thanks. From arvidsonkristen <@t> yahoo.com Mon Mar 2 13:18:38 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Mar 2 13:18:41 2009 Subject: [Histonet] distilled water Message-ID: <543714.42635.qm@web65714.mail.ac4.yahoo.com> Are most people using distilled water in the water baths? From leiker <@t> buffalo.edu Mon Mar 2 14:14:43 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Mar 2 14:14:50 2009 Subject: [Histonet] distilled water In-Reply-To: <543714.42635.qm@web65714.mail.ac4.yahoo.com> References: <543714.42635.qm@web65714.mail.ac4.yahoo.com> Message-ID: <07C7982C3F1B85C10E84BB98@bchwxp2702.ad.med.buffalo.edu> ummm...distilled water works best, unless you like cleaning out mineral deposit build-up and such... --On Monday, March 02, 2009 11:18 AM -0800 kristen arvidson wrote: > Are most people using distilled water in the water baths? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvienienced! From rjbuesa <@t> yahoo.com Mon Mar 2 15:08:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 2 15:08:05 2009 Subject: [Histonet] HIER In-Reply-To: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> Message-ID: <460718.52843.qm@web65708.mail.ac4.yahoo.com> Both temperature and pH go hand in hand and it is very difficult, if not impossible, to select one over the other without experimental data. The thing is to just boil the container with the pH buffer and once it has boiled (usually in 20 minutes) take it out and leave it on the counter for another 20 minutes. The pH (from 6 to 8) will depend on the epitope that is going to be retrieve because some need pH6 and other pH8 and even higher (or lower). Seldom pH7 (neutral) is used. Ren? J. --- On Mon, 3/2/09, Casper Hempel wrote: From: Casper Hempel Subject: [Histonet] HIER To: histonet@lists.utsouthwestern.edu Date: Monday, March 2, 2009, 1:52 PM Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 2 15:10:25 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 2 15:10:38 2009 Subject: [Histonet] Distilled water in the baths? In-Reply-To: <234672.67372.qm@web65711.mail.ac4.yahoo.com> Message-ID: <72234.69443.qm@web65711.mail.ac4.yahoo.com> We always used tap water with gelatin?in the water bath for regular sectioning, but for IHC procedures we used distilled water without any gelatin. Ren? J. --- On Mon, 3/2/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Distilled water in the baths? To: "histonet" Date: Monday, March 2, 2009, 2:10 PM Are people using tap water in their waterbaths? Has anyone noticed any problems with this? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pritchm <@t> ccf.org Mon Mar 2 15:11:35 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Mon Mar 2 15:11:50 2009 Subject: [Histonet] HIER In-Reply-To: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021513B3@CCHSCLEXMB56.cc.ad.cchs.net> Casper: I have a reproducibly good method for HEIR for two Abs I routinely use (alpha smooth muscle actin in mouse liver and proliferating cell nuclear antigen (PCNA) in mouse liver). I use a microwave pressure cooker (Nordic Ware, tender cooker). Into the pressure cooker, I place 300mL of distilled water to keep the humidity high during the in my pressure cooker. I use a citrate buffer solution which contains 0.01M sodium citrate dihydrate and 0.04M citric acid monohydrate, pH 6.0. I place 500mL of this solution into a 1.5pt (710mL) 'servin' saver' container and set the filled container into the pressure cooker (into the distilled water). I slip my slides into an autostainer rack and place the rack on its side in the citrate buffer soln. I microwave the entire setup at 50% power (1100 watt microwave) for 20 minutes and then allow the cooker to cool down for 20 additional minutes. When I remove the cooker from the microwave, I open it on my bench and take the temperature of the citrate buffer. It is usually between 95-98C. After the 20 minute cool-down, the temperature of the citrate buffer is around 56-57C. Once completely cool, I test the pH of the citrate buffer to ensure that it is still at pH 6.0. (I have taken these measurements a total of 11 times.) My single failure when using this method of HIER was when the temperature did not get hot enough. I was rather na?ve at the time and thought I would proceed with the staining and see what happened, assuming the pH was more important than the temperature. So much for that hypothesis. Mind you, I fully disclose that my experience is rather limited to the two staining protocols I have optimized, but I offer up that experience for your edification. Hope it helps! Kind regards: ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Casper Hempel Sent: Monday, March 02, 2009 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From janet.dertien <@t> ttuhsc.edu Mon Mar 2 15:45:04 2009 From: janet.dertien <@t> ttuhsc.edu (Dertien, Janet) Date: Mon Mar 2 15:45:08 2009 Subject: [Histonet] Manual for Nikon 300 diaphot Message-ID: Hello, Does anyone have a manual for a Nikon Diaphot 300? I found a previous similar request by Necat Yilmaz, and a positive response by Stacey Barick. Your help would be much appreciated! Thanks, Janet Janet Dertien Faculty Associate Texas Tech University Health Science Center Pharmacology & Neuroscience 3601 4th Street / STOP 6592 Lubbock, TX 79430 806-743-2425 x236 806-743-2744 From leiker <@t> buffalo.edu Mon Mar 2 16:06:37 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Mar 2 16:06:43 2009 Subject: [Histonet] HIER In-Reply-To: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> References: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> Message-ID: <77BDDB64167F2E874758C34C@bchwxp2702.ad.med.buffalo.edu> In our experience, the heat is definitely more important...we retrieve epitopes ranging from cytoskeletal markers (Troponins, Myosin Heavy Chain,...) to nucler (PCNA) and others (c-kit, beta-catenin, vonWillebrand Factor-vWF). We use a steamer set to 30', just place the slides in flat on the bottom of the basket: encircle the sections with Pap pen first, then pipette some buffer on. I always test pH 6 and pH 9 but haven't seen a difference yet between the two with the epitopes we're unmasking.) We have this wicked vWF antibody from Calbiochem (PC313) that unmasks in PBS (pH7) in the steamer and binds very well on both paraffins and frozens. (And it does need retrieval - without retrieval it is gives very little stain). Ok, I digress!! But you get the point...that's from our experience, anyway! ~Merced --On Monday, March 02, 2009 7:52 PM +0100 Casper Hempel wrote: > Hi histonetters > We have been talking a lot about how to retrieve your epitopes using a > microwave in the best possible way. > We haven't come to an agreement and people in my lab both argue that > temperature and pH are important issues. Without a doubt both factors are > important for a proper retrieval, but if you have to focus on one of the > factors, which would you consider the most important? Temperature or pH? > The issue is mainly longer incubations of the slides in boiling buffer. > The buffer is evaporating and the solution/buffer gets less and less pH > neutral and you need to top up with dH2O. However, if you boil with > reduced intensity, less evaporation will occur and the pH will remain > more stable. Do you have any suggestions or comments to this issue? > Looking forward to your replies. > Cheers > Casper > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvienienced! From louise.renton <@t> gmail.com Tue Mar 3 01:10:23 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Mar 3 01:10:31 2009 Subject: [Histonet] distilled water In-Reply-To: <543714.42635.qm@web65714.mail.ac4.yahoo.com> References: <543714.42635.qm@web65714.mail.ac4.yahoo.com> Message-ID: Dear Kirsten I've tried both, and find that tap water (presumably from all the aeration in the pipes) makes bubbles on the side of the bath that are ALWAYS released when you are cuting difficult section like lymph node or spleen.Of course you can tap the water bath to release the bubbles, but it looks a bit strange to be seen giving your unsuspecting bath a smart rap every so often. So, my 2c (recesion adjusted) worth - use dist H2O. On 3/2/09, kristen arvidson wrote: > > Are most people using distilled water in the water baths? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From SharonC <@t> celligent.net Tue Mar 3 07:04:52 2009 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Tue Mar 3 07:05:04 2009 Subject: [Histonet] Histotech position Message-ID: Good morning everyone! We have a position available in the Charlotte, NC for an ASCP certified Histotech (HT/or HTL). The hours are from 11AM-7PM. This person will need to have IHC/ISH experience as well as be able to gross (process) small specimens, mostly derms and GYN (ECC's, cervical Bx's). Please send resume's to sharonc@celligent.net or fax them to 704-549-0559 attn. Sharon Campbell. Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net From vjp2105 <@t> columbia.edu Tue Mar 3 08:14:06 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Mar 3 08:14:12 2009 Subject: [Histonet] Section thickness Message-ID: Hi Guys, Just wondering what thickness you cut sections at? I was always used to cutting at 2-3 microns in my last lab, however in my new place they are cutting at 6 microns (for both H & Es and IHC), which seems to me as really quite thick! What would be the average cutting thickness? Thanks a mill, Vanessa From barrickstacey <@t> yahoo.com Tue Mar 3 08:29:03 2009 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Tue Mar 3 08:29:08 2009 Subject: [Histonet] Manual for Nikon 300 diaphot In-Reply-To: Message-ID: <937535.54778.qm@web54305.mail.re2.yahoo.com> Hi Janet, ? I have sent you an email with the manual attached as a pdf. ? Stacey --- On Mon, 3/2/09, Dertien, Janet wrote: From: Dertien, Janet Subject: [Histonet] Manual for Nikon 300 diaphot To: histonet@lists.utsouthwestern.edu Date: Monday, March 2, 2009, 4:45 PM Hello, Does anyone have a manual for a Nikon Diaphot 300? I found a previous similar request by Necat Yilmaz, and a positive response by Stacey Barick. Your help would be much appreciated! Thanks, Janet Janet Dertien Faculty Associate Texas Tech University Health Science Center Pharmacology & Neuroscience 3601 4th Street / STOP 6592 Lubbock, TX 79430 806-743-2425 x236 806-743-2744 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 3 08:37:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 3 08:37:46 2009 Subject: [Histonet] Section thickness In-Reply-To: Message-ID: <646210.28566.qm@web65710.mail.ac4.yahoo.com> Vanessa: Lymph nodes for cellular details (special request)?= 3 ?m H&E and all other special procedures = 5 ?m Sections for bone marrow and liver reticulum stain = 7 ?m Brain and central nervous system = 10 ?m ? Now a question, why do you want to know this? To have "ammunition" to challenge what is done in your new lab? Not a wise move. I don't think that they would mind if you cut thinner, but they will mind if you start bringing this issue about. Let your?thinner sections "speak for themselves". It will get the moment that "by example" your way will prevail. Ren? J. --- On Tue, 3/3/09, Vanessa J. Phelan wrote: From: Vanessa J. Phelan Subject: [Histonet] Section thickness To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 3, 2009, 9:14 AM Hi Guys, Just wondering what thickness you cut sections at? I was always used to cutting at 2-3 microns in my last lab, however in my new place they are cutting at 6 microns (for both H & Es and IHC), which seems to me as really quite thick! What would be the average cutting thickness? Thanks a mill, Vanessa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Mar 3 08:49:42 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 3 08:49:51 2009 Subject: [Histonet] Section thickness In-Reply-To: References: Message-ID: I've read that 4-5um is "average", and that's what I typically go by for paraffins, but then it could depend on tissue type as Rene recommended. I end up having to cut frozens at 8-10um because for some reason it's very difficult to cut any thinner on the cyrostat I use (an old Vibratome model). But doesn't seem to matter for our applications - we do mostly immunofluorescence & some H&E. Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. --On Tuesday, March 03, 2009 9:14 AM -0500 "Vanessa J. Phelan" wrote: > Hi Guys, > > Just wondering what thickness you cut sections at? I was always used to > cutting at 2-3 microns in my last lab, however in my new place they are > cutting at 6 microns (for both H & Es and IHC), which seems to me as > really quite thick! What would be the average cutting thickness? > > Thanks a mill, > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvienienced! From leiker <@t> buffalo.edu Tue Mar 3 08:51:35 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 3 08:51:43 2009 Subject: [Histonet] Section thickness In-Reply-To: References: Message-ID: ...I just want to add, I could see it being a problem only in the sense that thicker sections tend to give more background in your immunostaining...however, you could conversely get greater signal strength as well... --On Tuesday, March 03, 2009 9:14 AM -0500 "Vanessa J. Phelan" wrote: > Hi Guys, > > Just wondering what thickness you cut sections at? I was always used to > cutting at 2-3 microns in my last lab, however in my new place they are > cutting at 6 microns (for both H & Es and IHC), which seems to me as > really quite thick! What would be the average cutting thickness? > > Thanks a mill, > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvienienced! From shive003 <@t> umn.edu Tue Mar 3 09:16:33 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Mar 3 09:16:37 2009 Subject: [Histonet] Section thickness References: Message-ID: <1B9381E63A004E5880A53BB7A09069FF@auxs.umn.edu> 4 um here. Jan Shivers UMN VDL ----- Original Message ----- From: "Vanessa J. Phelan" To: Sent: Tuesday, March 03, 2009 8:14 AM Subject: [Histonet] Section thickness > Hi Guys, > > Just wondering what thickness you cut sections at? I was always used to > cutting at 2-3 microns in my last lab, however in my new place they are > cutting at 6 microns (for both H & Es and IHC), which seems to me as > really > quite thick! What would be the average cutting thickness? > > Thanks a mill, > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ronald.Houston <@t> nationwidechildrens.org Tue Mar 3 09:22:24 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Mar 3 09:23:14 2009 Subject: [Histonet] Section thickness In-Reply-To: <1B9381E63A004E5880A53BB7A09069FF@auxs.umn.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645517@chi2k3ms01.columbuschildrens.net> 3 microns, but 2 micron for lymphoid tissues and renal biopsies Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, March 03, 2009 10:17 AM To: Vanessa J. Phelan; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Section thickness 4 um here. Jan Shivers UMN VDL ----- Original Message ----- From: "Vanessa J. Phelan" To: Sent: Tuesday, March 03, 2009 8:14 AM Subject: [Histonet] Section thickness > Hi Guys, > > Just wondering what thickness you cut sections at? I was always used to > cutting at 2-3 microns in my last lab, however in my new place they are > cutting at 6 microns (for both H & Es and IHC), which seems to me as > really > quite thick! What would be the average cutting thickness? > > Thanks a mill, > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From carrolpb <@t> umdnj.edu Tue Mar 3 09:28:28 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Mar 3 09:30:24 2009 Subject: [Histonet] Section thickness In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21B645517@chi2k3ms01.columbuschildrens.net> References: <979FF5962E234F45B06CF0DB7C1AABB21B645517@chi2k3ms01.columbuschildrens.net> Message-ID: <49AD4C9C.1000404@umdnj.edu> > Just wondering what thickness you cut sections at? 3 microns for all paraffin... From kimtournear <@t> yahoo.com Tue Mar 3 09:48:28 2009 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Mar 3 09:48:34 2009 Subject: [Histonet] Re: Mohs technique In-Reply-To: <23409049.1235589675823.JavaMail.root@elwamui-lapwing.atl.sa.earthlink.net> Message-ID: <10226.53099.qm@web54203.mail.re2.yahoo.com> The derm office I workd in found a neat little gadget called a "cryo-embedder". It is sold by Belaire Instruments and works great. Better than trying to use cryomolds, slides, etc... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks!!!! --- On Wed, 2/25/09, whitmorel wrote: From: whitmorel Subject: [Histonet] Re: Mohs technique To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 25, 2009, 12:21 PM First of all, there is no apostrophe in Mohs. Sorry, being picky is a necessity for a Mohs tech. How are you mounting your tissue? Are you using a glass slide to mount the tissue? There are several different method out there for mounting tissue so you can be sure the entire epidermis is completely down and air bubbles are out of the tissue. You might have your surgeon contact the Mohs College and see about getting a trainer to come and work with you. With your background, the 2 days the College suggests would work great. The other alternative is to go and visit a trainer. If you go onto the website www.mohscollege.org you can find a list of Mohs histotech trainers. Lynn Whitmore HT(ASCP) Mohs Histotechnology Trainer - >------------------------------ > >Message: 17 >Date: Wed, 25 Feb 2009 06:56:12 -0800 (PST) >From: Steven Coakley >Subject: [Histonet] Moh's techniques >To: Histonet@lists.utsouthwestern.edu >Message-ID: <164204.1533.qm@web38206.mail.mud.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Good morning all, >? >I'm learning a new way to do Moh's.? Much more relaxed compared to how I did them years ago with a Pathologist looking over my shoulder while I attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand and the Dermatologist wanting the results "yesterday".? I'd like??to get some ideas as to techniques Moh's Techs are using out there that work well in assuring that one gets the entire skin edge.? I'd also Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. area. >? >Thanks everyone, >? >Steve >? > > > > >------------------------------ > >M _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vjp2105 <@t> columbia.edu Tue Mar 3 09:53:51 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Mar 3 09:53:57 2009 Subject: [Histonet] Section thickness In-Reply-To: <646210.28566.qm@web65710.mail.ac4.yahoo.com> Message-ID: Ammunition...no, was more just inquiring what the what the average would be and if there was any specific reason for thinner sections other that just preference. Thanks Vanessa On 3/3/09 9:37 AM, "Rene J Buesa" wrote: > Vanessa: > Lymph nodes for cellular details (special request) = 3 ?m > H&E and all other special procedures = 5 ?m > Sections for bone marrow and liver reticulum stain = 7 ?m > Brain and central nervous system = 10 ?m > > Now a question, why do you want to know this? To have "ammunition" to > challenge what is done in your new lab? Not a wise move. I don't think that > they would mind if you cut thinner, but they will mind if you start bringing > this issue about. Let your thinner sections "speak for themselves". It will > get the moment that "by example" your way will prevail. > Ren? J. > > --- On Tue, 3/3/09, Vanessa J. Phelan wrote: >> From: Vanessa J. Phelan >> Subject: [Histonet] Section thickness >> To: histonet@lists.utsouthwestern.edu >> Date: Tuesday, March 3, 2009, 9:14 AM >> >> Hi Guys, >> >> Just wondering what thickness you cut sections at? I was always used to >> cutting at 2-3 microns in my last lab, however in my new place they are >> cutting at 6 microns (for both H & Es and IHC), which seems to me as really >> quite thick! What would be the average cutting thickness? >> >> Thanks a mill, >> >> Vanessa >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From Julie_Schoenborn <@t> bradycorp.com Tue Mar 3 09:57:15 2009 From: Julie_Schoenborn <@t> bradycorp.com (Julie_Schoenborn@bradycorp.com) Date: Tue Mar 3 09:57:20 2009 Subject: [Histonet] Hologic Thin Prep 2000 Imaging Sytem Message-ID: Does anyone have a copy of the ocr-a font so lab can print slide labels with appropriate readable text for this this system? From Jackie.O'Connor <@t> abbott.com Tue Mar 3 09:57:48 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Mar 3 09:58:13 2009 Subject: [Histonet] Biocare intelliPATH In-Reply-To: Message-ID: Soliciting opinions on this IHC stainer. Good or bad. From lblazek <@t> digestivespecialists.com Tue Mar 3 10:13:11 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Mar 3 10:09:29 2009 Subject: [Histonet] Biocare intelliPATH In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390870C5D696@IBMB7Exchange.digestivespecialists.com> Jackie, I have Biocare's intelliPath and like it very much. It is an open system so I have the option to use any reagents I choose. You can continuously add stains through out the day even if you have a protocol running. Instillation, support and service has been great. You can contact me any time if you would like any additional information. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, March 03, 2009 10:58 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Biocare intelliPATH Soliciting opinions on this IHC stainer. Good or bad. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Tue Mar 3 10:41:52 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Tue Mar 3 10:42:23 2009 Subject: [Histonet] Re: specimen tracking from the OR Message-ID: <49ACFB60020000A80002CAE8@mail.luhcares.org> We too are a small hospital. And like Terri there is a book in the OP area that the specimens are logged into when dropped off and picked up. It is a standard Chain-of-Custody (COC) protocol. We have recently added a new process as we too had a sample missplaced when the Histology Lab was closed. Now after hours tissue is brought to the General lab and a COC log is filled out with the RN dripping off the specimen and the Lab Assistant accepting the specimen. To copy Terri, Crude and time consuming, yes....accurate, too. Regards, Matt Lunetta HT, (ASCP) Longmont United Hospital Message: 2 Date: Mon, 2 Mar 2009 13:28:22 -0500 From: "Terri Braud" Subject: [Histonet] RE: specimen tracking from the OR To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are a small hospital lab, too. There is a log book in the OR, where specimens are placed for Histo pick up. The circulating nurse puts a Patient sticker and handwrites all specimens removed from the patient, regardless of what they are, and whether or not they have been walked down, tubed, or waiting pickup. We make sure that all specimens we remove are listed in the book, then sign/date/time for them. Any specimen left for us and not in the book, or vice versa, we bring to the attention of the OR control desk. If we make a run and there are no specimens to pick up, then we still sign and date/time the log book as "no specimens". Any specimens brought to the department are checked before accepting. Crude and time consuming, yes....accurate, too. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 6. log book (Kathy Gorham) Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: "Kathy Gorham" Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right n ow we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. From jwatson <@t> gnf.org Tue Mar 3 10:59:59 2009 From: jwatson <@t> gnf.org (James Watson) Date: Tue Mar 3 11:00:45 2009 Subject: [Histonet] Temp Position In San Diego Ca. Message-ID: Would you like to spend the summer in Sunny San Diego Ca.? Job Description GNF is currently seeking a temporary Scientific Associate to join the Histology group (End of June 2009 through beginning of October 2009). Job Summary Performs routine, special staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications Associate's degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. Having the American Society of Clinical Pathologist (ASCP) certification as a Histology Technician is required. Applicants must demonstrate the ability to perform the essential functions of the job as outlined in the position description. Experience Applicant should have at least 2 years of experience in animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry. Essential Functions 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs microtomy on rotary microtome, cryostat. 4. Prepares dyes and solutions in order to perform special or complex procedures. 5. Have the background to do basic histochemical stains and enzymatic histochemical stains. Have the background in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning instrumentation. The Genomics Institute of the Novartis Research Foundation (GNF), located in the Torrey Pines area of San Diego, CA, is funded by the Novartis Research Foundation and dedicated to the development and application of new methods and techniques for genome-wide biological discovery and biomedical research. GNF provides a unique and challenging opportunity to combine exploratory biomedical research with pharmaceutical drug development in a highly interactive, multidisciplinary environment and state-of-the-art facilities. GNF offers excellent compensation and a great benefits package. Visit our website at www.gnf.org EOE Please submit your CV and any supporting documents to: Genomics Institute of the Novartis Research Foundation Job Code: JW03-003 10675 John Jay Hopkins Drive San Diego, CA 92121 Fax: 858/812-1670, or submit online to jobs@gnf.org (subject line must include JW03-003) From tbraud <@t> holyredeemer.com Tue Mar 3 11:15:15 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Mar 3 11:15:26 2009 Subject: [Histonet] Manager's question Message-ID: I need some histonet help, please. If you are a manager and if you are being asked to manage to "units per productive manhour" where a unit is one billed test, would you mind sharing your budgeted target? Also, if you have this information, please let me know what services your lab offers...Histology, IHC, Cytology? I've been asked to manage to this figure before, but we always had benchmarked figures to go by. Thanks in advance, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From foreightl <@t> gmail.com Tue Mar 3 13:35:57 2009 From: foreightl <@t> gmail.com (Pat Laurie) Date: Tue Mar 3 13:36:01 2009 Subject: [Histonet] Biocare intelliPATH In-Reply-To: References: Message-ID: We just got an IntelliPath installed. We are currently evaluating it Vs. Ventana's Benchmark. Currently, I have nothing but good things to say about it. The good things are Cold spot for reagents unstable at room temperature ?On-board reagent mixing vials ?50 slides split into 5 simultaneous runs Can run on a first in, first out basis, multiple 3 hour runs mean 150 slides finished in 9 hours Multiplex staining, (using the Biocare products) mean that dual or even more stains take the same time as a single stain, One of the best things is OPEN abilities. Can use reagents from any source ? Dako, Leica Lab Vision and Ventana, etc Full ability to modify detection protocol. Retrieval, buffering, and chromagen ancillaries can be from any possible kit or individual reagent. And I must concur, the Installation and support is excellent. If you need any further info from me, I would be happy to help. -- Patrick Laurie HT(ASCP)QIHC Cellnetix Seattle, Wa On Tue, Mar 3, 2009 at 7:57 AM, Jackie M O'Connor < Jackie.O'Connor@abbott.com > wrote: > Soliciting opinions on this IHC stainer. Good or bad. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bob.nienhuis <@t> gmail.com Tue Mar 3 13:45:07 2009 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Tue Mar 3 13:45:12 2009 Subject: [Histonet] Mercuric chloride SOP for Golgi Message-ID: <45109da50903031145w1d3e0f31lba968b2e99909e3f@mail.gmail.com> Anyone have a handy SOP for safe handling and disposal of Mercuric chloride as used in Golgi-Cox staining? This is for a VA research facility. Our safety guys think ours in inadequate. Bob Nienhuis VA / UCLA Medical Center From katherine-walters <@t> uiowa.edu Tue Mar 3 14:05:56 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Tue Mar 3 14:06:10 2009 Subject: [Histonet] Cresyl violet problems Message-ID: To the "brainy" histo-people, I have a student doing a cresyl violet stain on some frozen (40um) brain sections. He has been doing this routinely for a couple of months, but his last run looks very odd. There appears to be an area on each section where there is no staining. At first glance I thought it was a fixation problem, but the unstained portion of the tissue is in a different area as you move from slice to slice (these are serial sections), He stains in a Copeland jar and assures me that his stain is well homogenized. Could this be some kind of moisture problem? Thanks for your thoughts. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. From Marilyn.A.Weiss <@t> kp.org Tue Mar 3 14:08:52 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Mar 3 14:08:42 2009 Subject: [Histonet] Re: Histonet Digest, Vol 64, Issue 4 In-Reply-To: <200903031801.n23I1sRv013488@mail.kp.org> Message-ID: In regard to tracking OR specimens, we use a "handling transmittal " form. On it is the patient information, PF number, doctor, courier, who it was rc'd by and the specimen . The OR fills it out and whoever receives the specimens will check the paperwork against the name on the container . NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. histonet-request@lists.utsouthwestern.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 03/03/2009 10:01 AM Please respond to histonet@lists.utsouthwestern.edu To histonet@lists.utsouthwestern.edu cc Subject Histonet Digest, Vol 64, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Re: Mohs technique (Kim Tournear) 2. Re: Section thickness (Vanessa J. Phelan) 3. Hologic Thin Prep 2000 Imaging Sytem (Julie_Schoenborn@bradycorp.com) 4. Biocare intelliPATH (Jackie M O'Connor) 5. RE: Biocare intelliPATH (Blazek, Linda) 6. Re: specimen tracking from the OR (Matthew Lunetta) 7. Temp Position In San Diego Ca. (James Watson) 8. Manager's question (Terri Braud) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Mar 2009 07:48:28 -0800 (PST) From: Kim Tournear Subject: Re: [Histonet] Re: Mohs technique To: Histonet Message-ID: <10226.53099.qm@web54203.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The derm office I workd in found a neat little gadget called a "cryo-embedder". It is sold by Belaire Instruments and works great. Better than trying to use cryomolds, slides, etc... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! --- On Wed, 2/25/09, whitmorel wrote: From: whitmorel Subject: [Histonet] Re: Mohs technique To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 25, 2009, 12:21 PM First of all, there is no apostrophe in Mohs. Sorry, being picky is a necessity for a Mohs tech. How are you mounting your tissue? Are you using a glass slide to mount the tissue? There are several different method out there for mounting tissue so you can be sure the entire epidermis is completely down and air bubbles are out of the tissue. You might have your surgeon contact the Mohs College and see about getting a trainer to come and work with you. With your background, the 2 days the College suggests would work great. The other alternative is to go and visit a trainer. If you go onto the website www.mohscollege.org you can find a list of Mohs histotech trainers. Lynn Whitmore HT(ASCP) Mohs Histotechnology Trainer - >------------------------------ > >Message: 17 >Date: Wed, 25 Feb 2009 06:56:12 -0800 (PST) >From: Steven Coakley >Subject: [Histonet] Moh's techniques >To: Histonet@lists.utsouthwestern.edu >Message-ID: <164204.1533.qm@web38206.mail.mud.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Good morning all, > >I'm learning a new way to do Moh's. Much more relaxed compared to how I did them years ago with a Pathologist looking over my shoulder while I attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand and the Dermatologist wanting the results "yesterday". I'd like to get some ideas as to techniques Moh's Techs are using out there that work well in assuring that one gets the entire skin edge. I'd also Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. area. > >Thanks everyone, > >Steve > > > > > >------------------------------ > >M _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 3 Mar 2009 10:53:51 -0500 From: "Vanessa J. Phelan" Subject: Re: [Histonet] Section thickness To: , Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Ammunition...no, was more just inquiring what the what the average would be and if there was any specific reason for thinner sections other that just preference. Thanks Vanessa On 3/3/09 9:37 AM, "Rene J Buesa" wrote: > Vanessa: > Lymph nodes for cellular details (special request) = 3 ?m > H&E and all other special procedures = 5 ?m > Sections for bone marrow and liver reticulum stain = 7 ?m > Brain and central nervous system = 10 ?m > > Now a question, why do you want to know this? To have "ammunition" to > challenge what is done in your new lab? Not a wise move. I don't think that > they would mind if you cut thinner, but they will mind if you start bringing > this issue about. Let your thinner sections "speak for themselves". It will > get the moment that "by example" your way will prevail. > Ren? J. > > --- On Tue, 3/3/09, Vanessa J. Phelan wrote: >> From: Vanessa J. Phelan >> Subject: [Histonet] Section thickness >> To: histonet@lists.utsouthwestern.edu >> Date: Tuesday, March 3, 2009, 9:14 AM >> >> Hi Guys, >> >> Just wondering what thickness you cut sections at? I was always used to >> cutting at 2-3 microns in my last lab, however in my new place they are >> cutting at 6 microns (for both H & Es and IHC), which seems to me as really >> quite thick! What would be the average cutting thickness? >> >> Thanks a mill, >> >> Vanessa >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > ------------------------------ Message: 3 Date: Tue, 3 Mar 2009 09:57:15 -0600 From: Julie_Schoenborn@bradycorp.com Subject: [Histonet] Hologic Thin Prep 2000 Imaging Sytem To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Does anyone have a copy of the ocr-a font so lab can print slide labels with appropriate readable text for this this system? ------------------------------ Message: 4 Date: Tue, 3 Mar 2009 09:57:48 -0600 From: Jackie M O'Connor Subject: [Histonet] Biocare intelliPATH To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Soliciting opinions on this IHC stainer. Good or bad. ------------------------------ Message: 5 Date: Tue, 3 Mar 2009 11:13:11 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Biocare intelliPATH To: 'Jackie M O'Connor' , "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390870C5D696@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Jackie, I have Biocare's intelliPath and like it very much. It is an open system so I have the option to use any reagents I choose. You can continuously add stains through out the day even if you have a protocol running. Instillation, support and service has been great. You can contact me any time if you would like any additional information. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, March 03, 2009 10:58 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Biocare intelliPATH Soliciting opinions on this IHC stainer. Good or bad. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 03 Mar 2009 09:41:52 -0700 From: "Matthew Lunetta" Subject: [Histonet] Re: specimen tracking from the OR To: Message-ID: <49ACFB60020000A80002CAE8@mail.luhcares.org> Content-Type: text/plain; charset=US-ASCII We too are a small hospital. And like Terri there is a book in the OP area that the specimens are logged into when dropped off and picked up. It is a standard Chain-of-Custody (COC) protocol. We have recently added a new process as we too had a sample missplaced when the Histology Lab was closed. Now after hours tissue is brought to the General lab and a COC log is filled out with the RN dripping off the specimen and the Lab Assistant accepting the specimen. To copy Terri, Crude and time consuming, yes....accurate, too. Regards, Matt Lunetta HT, (ASCP) Longmont United Hospital Message: 2 Date: Mon, 2 Mar 2009 13:28:22 -0500 From: "Terri Braud" Subject: [Histonet] RE: specimen tracking from the OR To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are a small hospital lab, too. There is a log book in the OR, where specimens are placed for Histo pick up. The circulating nurse puts a Patient sticker and handwrites all specimens removed from the patient, regardless of what they are, and whether or not they have been walked down, tubed, or waiting pickup. We make sure that all specimens we remove are listed in the book, then sign/date/time for them. Any specimen left for us and not in the book, or vice versa, we bring to the attention of the OR control desk. If we make a run and there are no specimens to pick up, then we still sign and date/time the log book as "no specimens". Any specimens brought to the department are checked before accepting. Crude and time consuming, yes....accurate, too. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 6. log book (Kathy Gorham) Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: "Kathy Gorham" Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right n ow we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. ------------------------------ Message: 7 Date: Tue, 3 Mar 2009 08:59:59 -0800 From: "James Watson" Subject: [Histonet] Temp Position In San Diego Ca. To: Message-ID: Content-Type: text/plain; charset="us-ascii" Would you like to spend the summer in Sunny San Diego Ca.? Job Description GNF is currently seeking a temporary Scientific Associate to join the Histology group (End of June 2009 through beginning of October 2009). Job Summary Performs routine, special staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications Associate's degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. Having the American Society of Clinical Pathologist (ASCP) certification as a Histology Technician is required. Applicants must demonstrate the ability to perform the essential functions of the job as outlined in the position description. Experience Applicant should have at least 2 years of experience in animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry. Essential Functions 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs microtomy on rotary microtome, cryostat. 4. Prepares dyes and solutions in order to perform special or complex procedures. 5. Have the background to do basic histochemical stains and enzymatic histochemical stains. Have the background in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning instrumentation. The Genomics Institute of the Novartis Research Foundation (GNF), located in the Torrey Pines area of San Diego, CA, is funded by the Novartis Research Foundation and dedicated to the development and application of new methods and techniques for genome-wide biological discovery and biomedical research. GNF provides a unique and challenging opportunity to combine exploratory biomedical research with pharmaceutical drug development in a highly interactive, multidisciplinary environment and state-of-the-art facilities. GNF offers excellent compensation and a great benefits package. Visit our website at www.gnf.org EOE Please submit your CV and any supporting documents to: Genomics Institute of the Novartis Research Foundation Job Code: JW03-003 10675 John Jay Hopkins Drive San Diego, CA 92121 Fax: 858/812-1670, or submit online to jobs@gnf.org (subject line must include JW03-003) ------------------------------ Message: 8 Date: Tue, 3 Mar 2009 12:15:15 -0500 From: "Terri Braud" Subject: [Histonet] Manager's question To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I need some histonet help, please. If you are a manager and if you are being asked to manage to "units per productive manhour" where a unit is one billed test, would you mind sharing your budgeted target? Also, if you have this information, please let me know what services your lab offers...Histology, IHC, Cytology? I've been asked to manage to this figure before, but we always had benchmarked figures to go by. Thanks in advance, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 64, Issue 4 *************************************** From godsgalnow <@t> aol.com Tue Mar 3 14:09:59 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Mar 3 14:12:46 2009 Subject: [Histonet] Biocare intelliPATH In-Reply-To: Message-ID: <8CB6A532A061043-14A0-24E@Webmail-mg14.sim.aol.com> Excellent Roxanne -----Original Message----- From: Jackie M O'Connor To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Tue, 3 Mar 2009 10:57 am Subject: [Histonet] Biocare intelliPATH Soliciting opinions on this IHC stainer. Good or bad. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victoria.spoon <@t> bassett.org Tue Mar 3 14:16:41 2009 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Tue Mar 3 14:16:47 2009 Subject: [Histonet] Cassette holders Message-ID: <415700FC732DE14491A3E39367834F77022277E0@ex3.bassett.org> Does anyone know where to purchase cassette holders? Plastic or metal holders for cassettes - 5 across and 4- 5 deep. Thanks, From awatanabe <@t> tgen.org Tue Mar 3 14:22:33 2009 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Tue Mar 3 14:22:41 2009 Subject: [Histonet] Mucin staining Message-ID: Someone please refresh my memory. I am looking for a mucin stain to stain cell lines embedded in agarose then formalin fixed and embedded in paraffin. I know mucicarmine might work, but I?ve also heard about using alcian blue. Can anyone point me in the right direction. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From rennie1108 <@t> yahoo.com Tue Mar 3 14:27:36 2009 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Tue Mar 3 14:27:41 2009 Subject: [Histonet] Storage of muscles Message-ID: <531150.56169.qm@web59607.mail.ac4.yahoo.com> Hello, We are having problems with artifact after our muscles have been stored for long periods of time. Can anyone tell me how they store their muscle specimens for long-term? ? Thank you, Adrienne Anderson Duke University Health System rennie1108@yahoo.com ? From Marilyn.A.Weiss <@t> kp.org Tue Mar 3 14:35:45 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Mar 3 14:35:14 2009 Subject: [Histonet] PA needed Message-ID: Kaiser Hospital in San Diego is in need of a PA to gross in large specimens, help with frozen sections and help with autopsies on a rotating system.Please contact Diane I Johnson at 619-528-5386 or by e-mail Diane. I .Johnson@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank y From lwenk <@t> sbcglobal.net Tue Mar 3 14:34:58 2009 From: lwenk <@t> sbcglobal.net (Lee Wenk) Date: Tue Mar 3 14:35:15 2009 Subject: [Histonet] Sulfated Alcian Blue In-Reply-To: <116774.23931.qm@web83908.mail.sp1.yahoo.com> References: <116774.23931.qm@web83908.mail.sp1.yahoo.com> Message-ID: <5A683B430E384A4288053DE590533B70@LeeWenkPC> Sulfonate Alcian Blue (SAB) is not always as specific as you would like. Also, if you are using the newer alcian blue dye, it seems to have a different formulation than the old stuff that I have. The new alcian blue will not dissolve in alcohol, so the newer dye doesn't demonstrate the amyloid, while my old dye does. Old deposits of amyloid do not have beta pleats at regular intervals. Therefore, the Congo red dye (CR) will still bind, but will not line up one right after the other | | | | |, but will be more random \ _ | /. When the CR dye are parallel to each other, they will show the apple green birefringence with the polarizing microscope. When the CR dye is randomly arranged, there will be no apple green birefringence. The other time this happens is with very overfixed amyloid, such as months in NBF. Too many cross-links with NBF, so the CR dye can't bind right, so they are not in parallel. We also had this happen once when the autopsy tissues were fixed in B5 (mercury fixative, long time ago). The resident knew amyloid was an immunological problem, so put through tissue fixed in B5. Congo red did not birefringe. So we went back to the NBF stock bucket, submitted new tissue, and they all were wonderfully green birefringent. The other problem with the SAB is that, if this is old amyloid, and the beta pleats are messed up, SAB depends somewhat on the beta pleats. Therefore, the green will be much paler in older amyloid than with newer amyoid. (The green is due to to the blue of alcian blue staining the amyloid, and the yellow of picric acid in the van Gieson also staining the amyloid, so blue and yellow make green.) We just had a case of non-birefringing green Congo red at our hospital a couple of months ago, where it definitely looked by amyloid on the H&E, but there was no birefringence on the patient's CR (control was great - all 3 times that we repeated the procedure). Ot wasn't a fixation problem, or a staining problem, but probably an "old amyloid" problem. Our resident Dr. Tom Fennel just gave a talk on it at our state histology's winter seminar Jan. 31, 2009. Here's what we did (all three): 1. View the Congo Red stained slides with a fluorescence microscope, such as in microbiology auramine-rhodamine stain for AFB. When hit with green light, the CR stained amyloid will fluoresce orange. 2. Use Crystal Violet or Methyl violet staining for amyloid. These depend upon the carboxyl ions of the amyloid for binding, not the beta pleats. The amyloid should be violet, with the background blue/purple. However, it doesn't always demonstrate AA amyloid (some are low in surface carboxyl ions). So not always as sensitive as CR. 3. Use Thioflavin T or Thioflavin S for amyloid. Staining is (maybe) with the P component of amyloid, not the beta pleats. Use a fluorescence microsope, using blue light (FITC filters), and the amyloid will fluoresce yellow. However, other things also fluoresce yellow, such as fibrinoid material, JG granules, sometimes elastin, etc. So not as specific as CR. By knowing your histology and knowing where in the tissue you are thinking that there are amyloid deposits, the above three alternatives are a nice addition, for when Congo red is not demonstrating the apple green birefringence. Since in our recent case, 3 out of 4 stains demonstrated amyloid (CR fluorescent, Crystal violet and TFT were all positive, while CR birefringence was negative), it was diagnosed as amyloid, with, I think, a note that older deposits of amyloid sometimes demonstrate no birefringence with CR. If this person needs help with find staining procedures for CV/MV or TFT/TFS, could someone email them? I'm on vacation, and don't have access to my home or work computer. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Nathanial nauss" To: Sent: Friday, February 27, 2009 10:42 AM Subject: [Histonet] Sulfated Alcian Blue I need some help, has any one used the sulfated alcian blue to stain for amyloid. We have a case that looks like it should be positive but it is not staining with the Congo Red. Any help would be great. Nathaniel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Mar 3 14:33:45 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Mar 3 14:36:30 2009 Subject: [Histonet] distilled water References: <543714.42635.qm@web65714.mail.ac4.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2930@fhosxchmb006.ADVENTISTCORP.NET> Try laying a glass slide down in the bottom of the waterbath after you swish the existing bubbles off the sides and bottom of the bath....watch the bubbles behave! No - I don't know why. Janet Janet L. Bonner, HTL (ASCP) Pathology Laboratory ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of louise renton Sent: Tue 3/3/2009 2:10 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] distilled water Dear Kirsten I've tried both, and find that tap water (presumably from all the aeration in the pipes) makes bubbles on the side of the bath that are ALWAYS released when you are cuting difficult section like lymph node or spleen.Of course you can tap the water bath to release the bubbles, but it looks a bit strange to be seen giving your unsuspecting bath a smart rap every so often. So, my 2c (recesion adjusted) worth - use dist H2O. On 3/2/09, kristen arvidson wrote: > > Are most people using distilled water in the water baths? > > > > _______________________________________________ ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From gmartin <@t> marshallmedical.org Tue Mar 3 14:36:07 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Mar 3 14:37:11 2009 Subject: [Histonet] Cassette holders In-Reply-To: <415700FC732DE14491A3E39367834F77022277E0@ex3.bassett.org> References: <415700FC732DE14491A3E39367834F77022277E0@ex3.bassett.org> Message-ID: <6ED9D4252F278841A0593D3D788AF24C04ADE25E@mailsvr.MARSHMED.local> Yes .. Hacker instruments www.hackerinstruments.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Spoon, Victoria Sent: Tuesday, March 03, 2009 12:17 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Cassette holders Does anyone know where to purchase cassette holders? Plastic or metal holders for cassettes - 5 across and 4- 5 deep. Thanks, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ebreisch <@t> rchsd.org Tue Mar 3 14:39:54 2009 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Tue Mar 3 14:40:01 2009 Subject: [Histonet] bone histology and storage Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B303A52F28@e2k3backend1.RCHSD.org> Does anyone have a methodology for long term storage of bone? Formalin vs alcohol? Orthopedic group here is wondering whether bone for research purposes should be preferentially stored in alcohol. They aren't interested in immunohistochemistry but rather tensile properties of bone. I haven't been able to find any information on the advantages or disadvantages of bone storage with formalin vs alcohol and hope that someone may be able to shed light on this issue or direct me to the appropriate information site. Thanks in advance for your help. Eric Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine From lwenk <@t> sbcglobal.net Tue Mar 3 14:44:35 2009 From: lwenk <@t> sbcglobal.net (Lee Wenk) Date: Tue Mar 3 14:44:48 2009 Subject: [Histonet] Mucin staining In-Reply-To: References: Message-ID: What type of mucin? What type of cell? What is it's origin? PAS will stain neutral mucin. Mucicarmine works better on epithelial acid mucins, rather than connective tissue acid mucins. But it will stain both sulfated and carboxylated acid mucins. Alcian blue (pH 2.5) and colloidal iron work well on both epithelial and connective tissue acid mucins, and again on both sulfated and carboxylated acid mucins. Alcian blue at pH 1.0 or lower) stains sulfated, but not carboxylated, acid mucins, both epithelial and connective tissue mucins. High Iron Diamine (HID) stains sulfated acid mucins, of both epithelial and connective tissue origin, but not carboxylated mucins. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Aprill Watanabe" To: Sent: Tuesday, March 03, 2009 12:22 PM Subject: [Histonet] Mucin staining Someone please refresh my memory. I am looking for a mucin stain to stain cell lines embedded in agarose then formalin fixed and embedded in paraffin. I know mucicarmine might work, but I?ve also heard about using alcian blue. Can anyone point me in the right direction. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Tue Mar 3 14:56:07 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Tue Mar 3 14:56:43 2009 Subject: [Histonet] RE: bone histology and storage In-Reply-To: <43B97B4C402C2C44AAA2A8D2C86A88B303A52F28@e2k3backend1.RCHSD.org> References: <43B97B4C402C2C44AAA2A8D2C86A88B303A52F28@e2k3backend1.RCHSD.org> Message-ID: <5B6165D78AC14544974A844787B47E3804BEF234D4@MAIL5.ad.uams.edu> We wrap the bone in saline soaked gauze and freeze at -20 degrees C for bone strength testing. If we receive bone for histomorphometric analysis we store our bones in 100% Alcohol. If the Bones are for paraffin sectioning we fix for at least 3 days wash in running tap water and store in 70% EtOh. We prefer to go ahead and process the specimens into paraffin blocks but if the specimens are going to be used for something else then we take them to 70% EtOh. For Bone Mechanical testing which I believe you are looking for. By removing the bone, wrapping in saline soaked gauze and freezing does not hurt the tensile strength. When we get ready to test for 3 point bending,etc we thaw the bone test and then destroy. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breisch, Eric Sent: Tuesday, March 03, 2009 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone histology and storage Does anyone have a methodology for long term storage of bone? Formalin vs alcohol? Orthopedic group here is wondering whether bone for research purposes should be preferentially stored in alcohol. They aren't interested in immunohistochemistry but rather tensile properties of bone. I haven't been able to find any information on the advantages or disadvantages of bone storage with formalin vs alcohol and hope that someone may be able to shed light on this issue or direct me to the appropriate information site. Thanks in advance for your help. Eric Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From casperhempel <@t> gmail.com Tue Mar 3 15:12:34 2009 From: casperhempel <@t> gmail.com (Casper Hempel) Date: Tue Mar 3 15:12:40 2009 Subject: [Histonet] HIER In-Reply-To: <460718.52843.qm@web65708.mail.ac4.yahoo.com> References: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> <460718.52843.qm@web65708.mail.ac4.yahoo.com> Message-ID: <7b7a6a600903031312h3d757a65r507b567e0c9f1f00@mail.gmail.com> Thanks for all your useful comments. It sounds like to choose temperature over pH depends on the epitope in question more than anything else. We'll probably have to optimize it for each antigen. Best regards Casper 2009/3/2 Rene J Buesa > Both temperature and pH go hand in hand and it is very difficult, if not > impossible, to select one over the other without experimental data. > The thing is to just boil the container with the pH buffer and once it has > boiled (usually in 20 minutes) take it out and leave it on the counter for > another 20 minutes. > The pH (from 6 to 8) will depend on the epitope that is going to be > retrieve because some need pH6 and other pH8 and even higher (or lower). > Seldom pH7 (neutral) is used. > Ren? J. > > --- On *Mon, 3/2/09, Casper Hempel * wrote: > > From: Casper Hempel > Subject: [Histonet] HIER > To: histonet@lists.utsouthwestern.edu > Date: Monday, March 2, 2009, 1:52 PM > > Hi histonetters > We have been talking a lot about how to retrieve your epitopes using a > microwave in the best possible way. > We haven't come to an agreement and people in my lab both argue that > temperature and pH are important issues. Without a doubt both factors are > important for a proper retrieval, but if you have to focus on one of the > factors, which would you consider the most important? Temperature or pH? > The issue is mainly longer incubations of the slides in boiling buffer. The > buffer is evaporating and the solution/buffer gets less and less pH neutral > and you need to top up with dH2O. However, if you boil with reduced > intensity, less evaporation will occur and the pH will remain more stable. > Do you have any suggestions or comments to this issue? > Looking forward to your replies. > Cheers > Casper > _______________________________________________ > Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From dellav <@t> musc.edu Tue Mar 3 15:58:42 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Mar 3 15:58:48 2009 Subject: [Histonet] HIER In-Reply-To: <7b7a6a600903031312h3d757a65r507b567e0c9f1f00@mail.gmail.com> References: <7b7a6a600903021052r464b73a7wde8670d65b740416@mail.gmail.com> <460718.52843.qm@web65708.mail.ac4.yahoo.com> <7b7a6a600903031312h3d757a65r507b567e0c9f1f00@mail.gmail.com> Message-ID: You will definitely have to optimize for each antigen. The time your slides are in your retrieval buffer is inversely related to the temperature. The higher the temperature of your buffer, the shorter the time needed for retrieval. There is nothing wrong with retrieving at a lower temperature (lower than boiling) however you will have to significantly increase the time in order to attain a satisfactory result. If time is not an issue for you, you may be able to retrieve at 90-95 degrees (below boiling to avoid boiling off your buffer) but you may have to double or even triple the time your slides are left in the retrieval buffer at that lower temperature. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Casper Hempel Sent: Tuesday, March 03, 2009 4:13 PM To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HIER Thanks for all your useful comments. It sounds like to choose temperature over pH depends on the epitope in question more than anything else. We'll probably have to optimize it for each antigen. Best regards Casper 2009/3/2 Rene J Buesa > Both temperature and pH go hand in hand and it is very difficult, if not > impossible, to select one over the other without experimental data. > The thing is to just boil the container with the pH buffer and once it has > boiled (usually in 20 minutes) take it out and leave it on the counter for > another 20 minutes. > The pH (from 6 to 8) will depend on the epitope that is going to be > retrieve because some need pH6 and other pH8 and even higher (or lower). > Seldom pH7 (neutral) is used. > Ren? J. > > --- On *Mon, 3/2/09, Casper Hempel * wrote: > > From: Casper Hempel > Subject: [Histonet] HIER > To: histonet@lists.utsouthwestern.edu > Date: Monday, March 2, 2009, 1:52 PM > > Hi histonetters > We have been talking a lot about how to retrieve your epitopes using a > microwave in the best possible way. > We haven't come to an agreement and people in my lab both argue that > temperature and pH are important issues. Without a doubt both factors are > important for a proper retrieval, but if you have to focus on one of the > factors, which would you consider the most important? Temperature or pH? > The issue is mainly longer incubations of the slides in boiling buffer. The > buffer is evaporating and the solution/buffer gets less and less pH neutral > and you need to top up with dH2O. However, if you boil with reduced > intensity, less evaporation will occur and the pH will remain more stable. > Do you have any suggestions or comments to this issue? > Looking forward to your replies. > Cheers > Casper > _______________________________________________ > Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Mar 3 15:58:50 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 3 15:59:03 2009 Subject: [Histonet] Storage of muscles In-Reply-To: <531150.56169.qm@web59607.mail.ac4.yahoo.com> Message-ID: Minus 70 degrees A normal minus 20 freezer tends to dessicate the tissue Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Wednesday, 4 March 2009 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of muscles Hello, We are having problems with artifact after our muscles have been stored for long periods of time. Can anyone tell me how they store their muscle specimens for long-term? ? Thank you, Adrienne Anderson Duke University Health System rennie1108@yahoo.com ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Mar 3 16:02:44 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 3 16:02:51 2009 Subject: [Histonet] Sulfated Alcian Blue In-Reply-To: <5A683B430E384A4288053DE590533B70@LeeWenkPC> Message-ID: Peggy, Have you tried the alcian blue tetrakis (methylpyridinium) chloride (Sigma, Cat. No.A4045, 90% dye content)? I would be interested to know whether this stains the amyloid better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Wenk Sent: Wednesday, 4 March 2009 7:35 AM To: Nathanial nauss; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sulfated Alcian Blue Sulfonate Alcian Blue (SAB) is not always as specific as you would like. Also, if you are using the newer alcian blue dye, it seems to have a different formulation than the old stuff that I have. The new alcian blue will not dissolve in alcohol, so the newer dye doesn't demonstrate the amyloid, while my old dye does. Old deposits of amyloid do not have beta pleats at regular intervals. Therefore, the Congo red dye (CR) will still bind, but will not line up one right after the other | | | | |, but will be more random \ _ | /. When the CR dye are parallel to each other, they will show the apple green birefringence with the polarizing microscope. When the CR dye is randomly arranged, there will be no apple green birefringence. The other time this happens is with very overfixed amyloid, such as months in NBF. Too many cross-links with NBF, so the CR dye can't bind right, so they are not in parallel. We also had this happen once when the autopsy tissues were fixed in B5 (mercury fixative, long time ago). The resident knew amyloid was an immunological problem, so put through tissue fixed in B5. Congo red did not birefringe. So we went back to the NBF stock bucket, submitted new tissue, and they all were wonderfully green birefringent. The other problem with the SAB is that, if this is old amyloid, and the beta pleats are messed up, SAB depends somewhat on the beta pleats. Therefore, the green will be much paler in older amyloid than with newer amyoid. (The green is due to to the blue of alcian blue staining the amyloid, and the yellow of picric acid in the van Gieson also staining the amyloid, so blue and yellow make green.) We just had a case of non-birefringing green Congo red at our hospital a couple of months ago, where it definitely looked by amyloid on the H&E, but there was no birefringence on the patient's CR (control was great - all 3 times that we repeated the procedure). Ot wasn't a fixation problem, or a staining problem, but probably an "old amyloid" problem. Our resident Dr. Tom Fennel just gave a talk on it at our state histology's winter seminar Jan. 31, 2009. Here's what we did (all three): 1. View the Congo Red stained slides with a fluorescence microscope, such as in microbiology auramine-rhodamine stain for AFB. When hit with green light, the CR stained amyloid will fluoresce orange. 2. Use Crystal Violet or Methyl violet staining for amyloid. These depend upon the carboxyl ions of the amyloid for binding, not the beta pleats. The amyloid should be violet, with the background blue/purple. However, it doesn't always demonstrate AA amyloid (some are low in surface carboxyl ions). So not always as sensitive as CR. 3. Use Thioflavin T or Thioflavin S for amyloid. Staining is (maybe) with the P component of amyloid, not the beta pleats. Use a fluorescence microsope, using blue light (FITC filters), and the amyloid will fluoresce yellow. However, other things also fluoresce yellow, such as fibrinoid material, JG granules, sometimes elastin, etc. So not as specific as CR. By knowing your histology and knowing where in the tissue you are thinking that there are amyloid deposits, the above three alternatives are a nice addition, for when Congo red is not demonstrating the apple green birefringence. Since in our recent case, 3 out of 4 stains demonstrated amyloid (CR fluorescent, Crystal violet and TFT were all positive, while CR birefringence was negative), it was diagnosed as amyloid, with, I think, a note that older deposits of amyloid sometimes demonstrate no birefringence with CR. If this person needs help with find staining procedures for CV/MV or TFT/TFS, could someone email them? I'm on vacation, and don't have access to my home or work computer. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Nathanial nauss" To: Sent: Friday, February 27, 2009 10:42 AM Subject: [Histonet] Sulfated Alcian Blue I need some help, has any one used the sulfated alcian blue to stain for amyloid. We have a case that looks like it should be positive but it is not staining with the Congo Red. Any help would be great. Nathaniel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From brett_connolly <@t> merck.com Tue Mar 3 16:09:45 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Mar 3 16:09:52 2009 Subject: [Histonet] looking for aVb3 antibody for IHC on FFPE tissue Message-ID: <63EA0607835FBA4689CEA9EA8B48269201BD6FB0@usctmx1141.merck.com> any favorites out there for integrin alphaV beta 3? Thanks Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sraibley <@t> yahoo.com Tue Mar 3 16:22:32 2009 From: sraibley <@t> yahoo.com (Susan Raibley) Date: Tue Mar 3 16:22:35 2009 Subject: [Histonet] Help with McDowell and Trump's Fixative 4F:1G for EM samples Message-ID: <866016.89272.qm@web56001.mail.re3.yahoo.com> Hello!? Can anyone give me some info on McDowell and Trump's fixative?? We have a protocol that requests the formulation of 4F:1G?of McDowell and Trump's fixative that we are going to be collecting?electron microscopy samples in.? Does anyone know if this is a specific formulation that must be made up,?or is it a common formulation that can be bought ready made?? Thanks!? ? Susan Bincsik BASi, Histology Technician From sccrshlly <@t> yahoo.com Tue Mar 3 18:22:26 2009 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Tue Mar 3 18:22:30 2009 Subject: [Histonet] Re: Log Book Message-ID: <998174.35043.qm@web90303.mail.mud.yahoo.com> Kathy, ? I worked in a hospital at one time where we were expected to pick up the specimens from the OR.? We actually created a log book that stayed in the OR.? The nurses simply placed a patient label in a spiral notebook and noted the specimens beside it.? When we went to pick them up, we checked each specimen off and put our initials, date and time in the book. We would not pick up any specimens with a discrepancy until the nursing staff corrected it.? In the instance you are describing, the specimens for said patient would not have been picked up until the nursing staff corrected the log book or found the specimen.? We did also date/time stamp the requisitions when we got back to the lab as well. ? Good luck and I hope you find your specimen :(?? ? Michelle Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T From sally.norton <@t> seattlechildrens.org Tue Mar 3 18:46:23 2009 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Tue Mar 3 18:47:35 2009 Subject: [Histonet] Re: Log Book In-Reply-To: <998174.35043.qm@web90303.mail.mud.yahoo.com> References: <998174.35043.qm@web90303.mail.mud.yahoo.com> Message-ID: <16E0693C7018C245959AC729FE66EDE52DBD3C@s107.childrens.sea.kids> Kathy We use the same method as Michelle described. We mark next to the patient name if there is more that one specimen. We do not take the specimen if there is any discrepancy. The OR has to fix it first and sign a form taking responsibility for the specimen. Sally Seattle Children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shelly Coker Sent: Tuesday, March 03, 2009 4:22 PM To: histonet@lists.utsouthwestern.edu; gorhamk@verizon.net Subject: [Histonet] Re: Log Book Kathy, ? I worked in a hospital at one time where we were expected to pick up the specimens from the OR.? We actually created a log book that stayed in the OR.? The nurses simply placed a patient label in a spiral notebook and noted the specimens beside it.? When we went to pick them up, we checked each specimen off and put our initials, date and time in the book. We would not pick up any specimens with a discrepancy until the nursing staff corrected it.? In the instance you are describing, the specimens for said patient would not have been picked up until the nursing staff corrected the log book or found the specimen.? We did also date/time stamp the requisitions when we got back to the lab as well. ? Good luck and I hope you find your specimen :(?? ? Michelle Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Children's Hospital and Regional Medical Center is now Seattle Children's. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mmarti <@t> cmrb.eu Wed Mar 4 02:51:39 2009 From: mmarti <@t> cmrb.eu (Marti Gaudes, Merce) Date: Wed Mar 4 02:50:01 2009 Subject: [Histonet] immunohistochemistry with CD markers Message-ID: Hello! Can anyone help us with CD markers? We want to use some CD markers on mouse testis, and the antibodies data sheets explain that only freeze samples, cryosectioned and post-fixed with acetone can be used. We tried to do this protocols, but without any result. We frozen the sample with liquid nitrogen, then we sectioned the sample at the cryostat, and we fixed the sections with acetone over the slides. The morphology was very damaged, and we couldn't obtain any staining. Does anyone explain us the details or tricks of the technique, to improve our results? Thanks a lot! Merc? Mart? Gaudes Cap de Servei Histology and Bioimaging Centre de Medicina Regenerativa de Barcelona (CMR[B]) Dr. Aiguader, 88 7ena planta 08003 Barcelona mmarti@cmrb.eu Tel: +34 93 316 03 51 Fax: +34 93 224 10 83 -------- Abans d'imprimir aquest missatge, si us plau, comprova que ??s realment necessari. El medi ambient ??s cosa de tots. Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. -------- La informaci?? continguda en aquest missatge i en qualsevol fitxer adjunt ??s confidencial, privada i d'??s exclusiu per al destinatari. Si no ??s la persona a la qual anava dirigida aquesta informaci??, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c??pia, divulgaci??, distribuci?? o utilitzaci?? no autoritzada d'aquest correu electr??nic i dels seus adjunts est?? prohibida en virtut de la legislaci?? vigent. La informaci??n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci??n, por favor, notifique inmediatamente el env??o err??neo al remitente y borre el mensaje. Cualquier copia, divulgaci??n, distribuci??n o utilizaci??n no autorizada de este correo electr??nico y de sus adjuntos est?? prohibida en virtud de la legislaci??n vigente. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. -------- From anh2006 <@t> med.cornell.edu Wed Mar 4 08:08:33 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Mar 4 08:11:10 2009 Subject: [Histonet] immunohistochemistry with CD markers In-Reply-To: References: Message-ID: <1335810618-1236175880-cardhu_decombobulator_blackberry.rim.net-1063902431-@bxe1028.bisx.prod.on.blackberry> What markers and specific clones do you need help with specifically? Also mouse testis really needs a PFA fix. Even if you do fresh frozen sections I suggest a short (10 min) fix in PFA rather than acetone. -----Original Message----- From: "Marti Gaudes, Merce" Date: Wed, 04 Mar 2009 09:51:39 To: Subject: [Histonet] immunohistochemistry with CD markers Hello! Can anyone help us with CD markers? We want to use some CD markers on mouse testis, and the antibodies data sheets explain that only freeze samples, cryosectioned and post-fixed with acetone can be used. We tried to do this protocols, but without any result. We frozen the sample with liquid nitrogen, then we sectioned the sample at the cryostat, and we fixed the sections with acetone over the slides. The morphology was very damaged, and we couldn't obtain any staining. Does anyone explain us the details or tricks of the technique, to improve our results? Thanks a lot! Merc? Mart? Gaudes Cap de Servei Histology and Bioimaging Centre de Medicina Regenerativa de Barcelona (CMR[B]) Dr. Aiguader, 88 7ena planta 08003 Barcelona mmarti@cmrb.eu Tel: +34 93 316 03 51 Fax: +34 93 224 10 83 -------- Abans d'imprimir aquest missatge, si us plau, comprova que ??s realment necessari. El medi ambient ??s cosa de tots. Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. -------- La informaci?? continguda en aquest missatge i en qualsevol fitxer adjunt ??s confidencial, privada i d'??s exclusiu per al destinatari. Si no ??s la persona a la qual anava dirigida aquesta informaci??, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c??pia, divulgaci??, distribuci?? o utilitzaci?? no autoritzada d'aquest correu electr??nic i dels seus adjunts est?? prohibida en virtut de la legislaci?? vigent. La informaci??n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci??n, por favor, notifique inmediatamente el env??o err??neo al remitente y borre el mensaje. Cualquier copia, divulgaci??n, distribuci??n o utilizaci??n no autorizada de este correo electr??nico y de sus adjuntos est?? prohibida en virtud de la legislaci??n vigente. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. -------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From farnhaml <@t> smha.org Wed Mar 4 08:11:22 2009 From: farnhaml <@t> smha.org (Farnham, Lori) Date: Wed Mar 4 08:11:29 2009 Subject: [Histonet] We are buying a new Tissue Processor and need imput Message-ID: <0D4634A987B07A4192E6F0F20D89B48301DBF341@ahcmascdc016.DS.SJHS.COM> Hi All, We are a small Pathology Lab which processes a little over 7000 surgical cases a year. We have a mixed variety of specimens (GI, GYN, skins, etc). We have been approved to buy a new tissue processor and are looking at the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these processors would be greatly appreciated. Thanks! Lori Ann Farnham, B.S.CT(ASCP) Cytotechnologist St. Mary's Hospital at Amsterdam Phone 518-841-7296 e-mail farnhaml@smha.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Mar 4 08:16:43 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Mar 4 08:16:47 2009 Subject: [Histonet] Storage of muscles In-Reply-To: References: <531150.56169.qm@web59607.mail.ac4.yahoo.com> Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A0B57FE@mailbe01.mc.vanderbilt.edu> I agree with Tony that a -70 freezer is ideal. We wrap our samples in aluminum foil and then place in a plastic container. If you only have access to a -20 freezer, it's helpful to place cold packs in the freezer, near where you will store the muscle samples. When a -20 freezer cycles, the temperature can fluctuate enough to create artifact. We've found that if we keep several disposable cold packs in a -20 that we can keep the temperature more consistent. Good Luck! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: Tuesday, March 03, 2009 3:59 PM To: rennie1108@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of muscles Minus 70 degrees A normal minus 20 freezer tends to dessicate the tissue Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Wednesday, 4 March 2009 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of muscles Hello, We are having problems with artifact after our muscles have been stored for long periods of time. Can anyone tell me how they store their muscle specimens for long-term? ? Thank you, Adrienne Anderson Duke University Health System rennie1108@yahoo.com ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From rjbuesa <@t> yahoo.com Wed Mar 4 08:26:55 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 4 08:26:59 2009 Subject: [Histonet] We are buying a new Tissue Processor and need imput In-Reply-To: <0D4634A987B07A4192E6F0F20D89B48301DBF341@ahcmascdc016.DS.SJHS.COM> Message-ID: <475563.58318.qm@web65704.mail.ac4.yahoo.com> I would by the VIP6 with total confidence. Ren? J. --- On Wed, 3/4/09, Farnham, Lori wrote: From: Farnham, Lori Subject: [Histonet] We are buying a new Tissue Processor and need imput To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 4, 2009, 9:11 AM Hi All, We are a small Pathology Lab which processes a little over 7000 surgical cases a year. We have a mixed variety of specimens (GI, GYN, skins, etc). We have been approved to buy a new tissue processor and are looking at the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these processors would be greatly appreciated. Thanks! Lori Ann Farnham, B.S.CT(ASCP) Cytotechnologist St. Mary's Hospital at Amsterdam Phone 518-841-7296 e-mail farnhaml@smha.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Wed Mar 4 08:37:08 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Mar 4 08:37:11 2009 Subject: [Histonet] distilled water...again Message-ID: <807905.47910.qm@web65716.mail.ac4.yahoo.com> How is everyone getting the water?? We have a distiller in the lab but it will need to be replaced soon.? The question is another distiller or buy bottled?? From leiker <@t> buffalo.edu Wed Mar 4 08:42:39 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 4 08:42:48 2009 Subject: [Histonet] distilled water...again In-Reply-To: <807905.47910.qm@web65716.mail.ac4.yahoo.com> References: <807905.47910.qm@web65716.mail.ac4.yahoo.com> Message-ID: it may be worth your while to buy another distiller...bottled water will cost more over time... --On Wednesday, March 04, 2009 6:37 AM -0800 kristen arvidson wrote: > How is everyone getting the water?? We have a distiller in the lab but it > will need to be replaced soon.? The question is another distiller or buy > bottled?? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From suhyoung.jeong <@t> gmail.com Wed Mar 4 09:55:47 2009 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Wed Mar 4 09:55:50 2009 Subject: [Histonet] Cholin acetyltransferase antibody Message-ID: <450012a20903040755w303a78dei8c270de672a3e44a@mail.gmail.com> Does anyone know a good anti-cholin acetyltransferase (ChAT) antibody for mouse spinal cord to do immunohistochemistry (frozen section)? I see a couple of vendors with more than a couple of clon names. Moreover, somebody recommended this neurotransmitter as a motor neuronal marker. If you can give me a better suggestion, it will be greatly appreciated. Thank you in advance!! Sincerely, Suh From tifei <@t> foxmail.com Wed Mar 4 09:58:41 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Mar 4 09:58:58 2009 Subject: [Histonet] Cresyl violet problems References: Message-ID: <200903042358363402037@foxmail.com> VHJ5IHNvbWUgZnJlc2hseSBwcmVwYXJlZCBjcmVzeWwgdmlvbGV0IHNvbHV0aW9ucz8NCg0KMjAw OS0wMy0wNCANCg0KDQoNClRGIA0KDQoNCg0Kt6K8/sjLo7ogV2FsdGVycywgS2F0aGVyaW5lIFMg DQq3osvNyrG85KO6IDIwMDktMDMtMDQgIDA0OjA5OjA3IA0KytW8/sjLo7ogaGlzdG9uZXRAcGF0 aG9sb2d5LnN3bWVkLmVkdSANCrOty82juiANCtb3zOKjuiBbSGlzdG9uZXRdIENyZXN5bCB2aW9s ZXQgcHJvYmxlbXMgDQogDQpUbyB0aGUgImJyYWlueSIgaGlzdG8tcGVvcGxlLA0KSSBoYXZlIGEg c3R1ZGVudCBkb2luZyBhIGNyZXN5bCB2aW9sZXQgc3RhaW4gb24gc29tZSBmcm96ZW4gKDQwdW0p IGJyYWluDQpzZWN0aW9ucy4gIEhlIGhhcyBiZWVuIGRvaW5nIHRoaXMgcm91dGluZWx5IGZvciBh IGNvdXBsZSBvZiBtb250aHMsIGJ1dA0KaGlzIGxhc3QgcnVuIGxvb2tzIHZlcnkgb2RkLiAgVGhl cmUgYXBwZWFycyB0byBiZSBhbiBhcmVhIG9uIGVhY2gNCnNlY3Rpb24gd2hlcmUgdGhlcmUgaXMg bm8gc3RhaW5pbmcuICBBdCBmaXJzdCBnbGFuY2UgSSB0aG91Z2h0IGl0IHdhcyBhDQpmaXhhdGlv biBwcm9ibGVtLCBidXQgdGhlIHVuc3RhaW5lZCBwb3J0aW9uIG9mIHRoZSB0aXNzdWUgaXMgaW4g YQ0KZGlmZmVyZW50IGFyZWEgYXMgeW91IG1vdmUgZnJvbSBzbGljZSB0byBzbGljZSAodGhlc2Ug YXJlIHNlcmlhbA0Kc2VjdGlvbnMpLCAgIEhlIHN0YWlucyBpbiBhIENvcGVsYW5kIGphciBhbmQg YXNzdXJlcyBtZSB0aGF0IGhpcyBzdGFpbg0KaXMgd2VsbCBob21vZ2VuaXplZC4gIENvdWxkIHRo aXMgYmUgc29tZSBraW5kIG9mIG1vaXN0dXJlIHByb2JsZW0/DQpUaGFua3MgZm9yIHlvdXIgdGhv dWdodHMuDQpLYXRoZXJpbmUgV2FsdGVycw0KSGlzdG9sb2d5IERpcmVjdG9yDQpDZW50cmFsIE1p Y3Jvc2NvcHkgUmVzZWFyY2ggRmFjaWxpdGllcw0KODUgRWNrc3RlaW4gTWVkaWNhbCBSZXNlYXJj aCBCdWlsZGluZw0KVW5pdmVyc2l0eSBvZiBJb3dhDQpJb3dhIENpdHksIElvd2EgNTIyNDItMTEw MQ0Ka2F0aGVyaW5lLXdhbHRlcnNAdWlvd2EuZWR1DQp3d3cudWlvd2EuZWR1L35jZW1yZg0KTm90 aWNlOiBUaGlzIFVJIEhlYWx0aCBDYXJlIGUtbWFpbCAoaW5jbHVkaW5nIGF0dGFjaG1lbnRzKSBp cyBjb3ZlcmVkIGJ5IHRoZSBFbGVjdHJvbmljIENvbW11bmljYXRpb25zIFByaXZhY3kgQWN0LCAx OCBVLlMuQy4gMjUxMC0yNTIxLCBpcyBjb25maWRlbnRpYWwgYW5kIG1heSBiZSBsZWdhbGx5IHBy aXZpbGVnZWQuICBJZiB5b3UgYXJlIG5vdCB0aGUgaW50ZW5kZWQgcmVjaXBpZW50LCB5b3UgYXJl IGhlcmVieSBub3RpZmllZCB0aGF0IGFueSByZXRlbnRpb24sIGRpc3NlbWluYXRpb24sIGRpc3Ry aWJ1dGlvbiwgb3IgY29weWluZyBvZiB0aGlzIGNvbW11bmljYXRpb24gaXMgc3RyaWN0bHkgcHJv aGliaXRlZC4gIFBsZWFzZSByZXBseSB0byB0aGUgc2VuZGVyIHRoYXQgeW91IGhhdmUgcmVjZWl2 ZWQgdGhlIG1lc3NhZ2UgaW4gZXJyb3IsIHRoZW4gZGVsZXRlIGl0LiAgVGhhbmsgeW91Lg0KX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkhpc3RvbmV0IG1h aWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlz dHMudXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQNCg== From nilfgaard <@t> comcast.net Wed Mar 4 10:08:04 2009 From: nilfgaard <@t> comcast.net (nilfgaard@comcast.net) Date: Wed Mar 4 10:08:07 2009 Subject: [Histonet] histo equipment for sale In-Reply-To: <1168878646.77251236182856052.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> Message-ID: <1191749273.77671236182884723.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> Hello histonetters, I have the following, in perfect working condition, items for sale: tissue processor VIP1000 benchtop, just refurbished Shandon 24-3 slide stainer Surgipath PC3001, PC3002 embedding center ducktless hood with new carbon filters hood the a super quite vent system (for outside venting) mini VWR hybridization oven TBS warter bath - like new ONLY, if you are seriously interested, email me at sightdog@comcast.net All pieces are located in Chicagoland. Pics available, can ship UPS ground. thanks Sightdog From BMolinari <@t> heart.thi.tmc.edu Wed Mar 4 10:18:22 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Mar 4 10:18:26 2009 Subject: [Histonet] contrast Message-ID: Hi, I have some mouse hindlimbs that have catheterized with Omnipaque. The Omnipaque contains 302 ml of iohexol which is equivalent to 140 mg of organic iodine per ml. The mice will be sacrificed immediately after being radiographed. They would like them to be processed in paraffin. I am wondering if this will have any effect on the muscle. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From KKay <@t> chr.ab.ca Wed Mar 4 10:22:57 2009 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Wed Mar 4 10:23:02 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 5 - CASSETTE HOLDERS In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494EE4@exbe.chr.ab.ca> Good Morning Victoria I believe Market Lab carries plastic cassette holders. Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory Chinook Regional Hospital 960 - 19 st South Lethbridge, Alberta, Canada T1J 1W5 (403) 388 - 6061 (Phone) (403) 388 - 6067 (Fax) Message: 6 Date: Tue, 3 Mar 2009 15:16:41 -0500 From: "Spoon, Victoria" Subject: [Histonet] Cassette holders To: Message-ID: <415700FC732DE14491A3E39367834F77022277E0@ex3.bassett.org> Content-Type: text/plain; charset="us-ascii" Does anyone know where to purchase cassette holders? Plastic or metal holders for cassettes - 5 across and 4- 5 deep. Thanks, - This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From Wanda.Smith <@t> HCAhealthcare.com Wed Mar 4 10:57:37 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Mar 4 11:29:04 2009 Subject: [Histonet] Lymph Nodes on Colon Cancers Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BAE6279C07@NADCWPMSGCMS03.hca.corpad.net> Good Morning, What is everyone doing to meet the recommendation from the American College of Surgeons and American Cancer Society regarding finding a minimum of 12 lymph nodes on colon cancer cases? Especially when there are not 12 lymph nodes to be found??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax From rjbuesa <@t> yahoo.com Wed Mar 4 11:40:04 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 4 11:40:09 2009 Subject: [Histonet] Lymph Nodes on Colon Cancers In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BAE6279C07@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <770850.75187.qm@web65712.mail.ac4.yahoo.com> If there are not 12 to be found, document what you did, how you did it, and how many you found. You cannot find what is not there, but make sure you tried your best. Ren? J. --- On Wed, 3/4/09, Smith Wanda wrote: From: Smith Wanda Subject: [Histonet] Lymph Nodes on Colon Cancers To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 4, 2009, 11:57 AM Good Morning, What is everyone doing to meet the recommendation from the American College of Surgeons and American Cancer Society regarding finding a minimum of 12 lymph nodes on colon cancer cases? Especially when there are not 12 lymph nodes to be found??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Wed Mar 4 12:21:43 2009 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Mar 4 12:21:47 2009 Subject: [Histonet] staining for elastic fiber of artery in rat lung Message-ID: <937896.88292.qm@web38006.mail.mud.yahoo.com> Hello histonetters, ? Would you teach me what is best stain for elastic fiber in rat lung except weigert's stain? ? I want to look at arteries. ? Thanks in advance, Weihua From drvet_anjan <@t> hotmail.com Wed Mar 4 13:04:32 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Wed Mar 4 13:04:37 2009 Subject: [Histonet] vector blue- xylene soluble- any alternatives Message-ID: hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag From binghammg <@t> yahoo.com Wed Mar 4 13:43:28 2009 From: binghammg <@t> yahoo.com (Matthew Bingham) Date: Wed Mar 4 13:43:31 2009 Subject: [Histonet] (no subject) Message-ID: <322914.55412.qm@web63507.mail.re1.yahoo.com> As many of you may or may not know, the Histobath countertop freezing bath has been discontinued by Thermo (formerly known as Thermo-Shandon, Shandon-Lipshaw, etc.). I was told that the freon that is used to allow the unit to cool to -60 is no longer allowed by the EPA to be used in ultra low refrigeration units. I know there is a floor model, the Clini-RF, at brightinstruments.com which uses a two types of freon in isolated side by side systems, but it is a rather large unit (20"D x 18"W x 40"H). Our frozen section room, as are most, is a small room with very limited space. Therefore, have any of you found a solution for a countertop model (i.e. 19"D x 9"W x 15"H)freezing bath to be used in frozen section rooms? I appreciate the help. Thanks, Matthew Bingham, PA(ASCP)CMAnatomic Pathology Supervisor Phoenix Children's Hospital Department of Pathology 1919 E. Thomas Road Phoenix, AZ 85016 phone: 602-546-1318 fax: 602-546-1284 email: mbingham@phoenixchildrens.com From Margaret.Perry <@t> sdstate.edu Wed Mar 4 15:04:23 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Mar 4 15:04:30 2009 Subject: [Histonet] problem eyes Message-ID: Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From rjbuesa <@t> yahoo.com Wed Mar 4 15:14:56 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 4 15:14:59 2009 Subject: [Histonet] problem eyes In-Reply-To: Message-ID: <240791.54814.qm@web65712.mail.ac4.yahoo.com> Add a few drops (4-5) of?liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). ren? J. --- On Wed, 3/4/09, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs <@t> yahoo.com Wed Mar 4 15:23:18 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Mar 4 15:23:23 2009 Subject: [Histonet] problem eyes Message-ID: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of?liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > ren? J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help!? We have been trying to cut whole eyes > from a pig.? They need to be > nice enough for a publication.? We are having the > devil of a time because no > matter what we do there are wrinkles.? If we turn the > waterbath up to stretch > things out the retina detaches.? Do any of you have > suggestions?? We uses > Surgipath? EM400 for embedding paraffin and and their > infiltration medium.? Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Mar 4 15:27:32 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 4 15:27:36 2009 Subject: [Histonet] problem eyes In-Reply-To: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Message-ID: <644825.64523.qm@web65712.mail.ac4.yahoo.com> Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Ren? J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of?liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > ren? J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help!? We have been trying to cut whole eyes > from a pig.? They need to be > nice enough for a publication.? We are having the > devil of a time because no > matter what we do there are wrinkles.? If we turn the > waterbath up to stretch > things out the retina detaches.? Do any of you have > suggestions?? We uses > Surgipath? EM400 for embedding paraffin and and their > infiltration medium.? Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Wed Mar 4 15:29:12 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 4 15:29:17 2009 Subject: [Histonet] problem eyes In-Reply-To: <787849.53788.qm@web46101.mail.sp1.yahoo.com> References: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Message-ID: <7FD71F250513F2B17D70DB05@bchwxp2702.ad.med.buffalo.edu> Good question! I'd like to know that, too! But with any size specimen, please! --On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello wrote: > > Really? Liquid laundry soap? Does this help with all wrinkles in large > specimens? > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Microscope Facility, room B141 > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Wed, 3/4/09, Rene J Buesa wrote: > >> From: Rene J Buesa >> Subject: Re: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> , "Perry, Margaret" >> Date: Wednesday, March 4, 2009, 9:14 PM >> Add a few drops (4-5) of?liquid >> detergent, but not dishwasher detergent (because it will >> dissolve the paraffin). >> ren? J. >> >> --- On Wed, 3/4/09, Perry, Margaret >> wrote: >> >> From: Perry, Margaret >> Subject: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> >> Date: Wednesday, March 4, 2009, 4:04 PM >> >> Please help!? We have been trying to cut whole eyes >> from a pig.? They need to be >> nice enough for a publication.? We are having the >> devil of a time because no >> matter what we do there are wrinkles.? If we turn the >> waterbath up to stretch >> things out the retina detaches.? Do any of you have >> suggestions?? We uses >> Surgipath? EM400 for embedding paraffin and and their >> infiltration medium.? Is >> this to soft? The lowest we can turn our waterbath is 38 >> degrees C. >> >> Margaret Perry HT (ASCP) >> IHC Lab Manager Veterinary Science >> Animal Disease Research and Diagnostic Lab >> South Dakota State University >> Box 2175 North Campus Drive >> Brookings SD 57007 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From vjp2105 <@t> columbia.edu Wed Mar 4 15:35:14 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Wed Mar 4 15:35:20 2009 Subject: [Histonet] Water bubble on slides Message-ID: Hi everyone, Quick question, I am finding after cutting a lot of slides for H&Es onto VWR Superfrost Plus slides (these are the only slides we have at the moment) that when I lift sections from the water bath ( of distilled water) a water bubble stays under the sections on the slide. When I lie them flat on the hot plate to dry the water bubble is tending to distort the tissue by escaping out through he tissue, eventually. I have not experienced this before, the water has ran off the slide no problem. Would it be the slides that are the problem or anything I might need to add to the water? I have just ordered a histology oven, this may be the solution...drying the slides standing up! Vanessa From brett_connolly <@t> merck.com Wed Mar 4 15:36:19 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Mar 4 15:36:26 2009 Subject: [Histonet] problem eyes In-Reply-To: References: Message-ID: <63EA0607835FBA4689CEA9EA8B48269201C38A0B@usctmx1141.merck.com> My suggestion would be to follow the procedure of the old ocular guru from the AFIP, Peter Emanuale. He taught me how the section human and rabbit eyes. It involves using 2 water baths, one of which is at room temp... and you should not use charged/coated slides. Check our his chapter on ocular histotechnology in the 1992 edition of the AFIP Lab Methods manual (it has step by step illustrations) Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, March 04, 2009 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem eyes Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pruegg <@t> ihctech.net Wed Mar 4 15:38:57 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Mar 4 15:39:05 2009 Subject: [Histonet] vector blue- xylene soluble- any alternatives In-Reply-To: References: Message-ID: <011DFB4829B140CE8DF5E5222EAE7497@prueggihctechlt> If I am not mistaken vectamount is an aqueous mountant, dpx is xylene/toluene based and requires going thru alcohols and xylene to mount. One thing I do and you can try it, is airdry the slides and the use permanent mounting media without going thru alcohols and xylene, keep in mind there is a little solvent in the media so it may have an effect. I do this with Fast Red substrate chromogen for AP have not tried it on vector blue. The other option is to use an aqueous mountant and seal the coverslip edges with clear nail polish. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, March 04, 2009 12:05 PM To: histonet-requests@lists.utsouthwestern.edu; triple immunohistochem Subject: [Histonet] vector blue- xylene soluble- any alternatives hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag______________________ _________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Mar 4 15:50:29 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 4 15:50:37 2009 Subject: [Histonet] Water bubble on slides In-Reply-To: References: Message-ID: Hi Vanessa, I have read in at least one online protocol and have also been advised by our excellent and experienced (former) histotech who used to be here at UB NOT to warm the slides without air-drying first. We typically air-dry the slides, yes, standing up, overnight, before warming them. Merced --On Wednesday, March 04, 2009 4:35 PM -0500 "Vanessa J. Phelan" wrote: > Hi everyone, > > Quick question, I am finding after cutting a lot of slides for H&Es onto > VWR Superfrost Plus slides (these are the only slides we have at the > moment) that when I lift sections from the water bath ( of distilled > water) a water bubble stays under the sections on the slide. When I lie > them flat on the hot plate to dry the water bubble is tending to distort > the tissue by escaping out through he tissue, eventually. I have not > experienced this before, the water has ran off the slide no problem. > Would it be the slides that are the problem or anything I might need to > add to the water? > > I have just ordered a histology oven, this may be the solution...drying > the slides standing up! > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From vapatpxs <@t> yahoo.com Wed Mar 4 16:39:33 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Mar 4 16:39:38 2009 Subject: [Histonet] Autotechnicon model 2A Message-ID: <969177.76764.qm@web46110.mail.sp1.yahoo.com> Hi All, Does anybody have an owner's manual to an Autotechnicon 2A? I've inherited this 42 year old beasty and can't remember how to work the delay timer. Yes, I am old enough to have used one of these R2D2 units in the past. If you can help me, I'd be much obliged. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From AnthonyH <@t> chw.edu.au Wed Mar 4 16:41:39 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Mar 4 16:41:50 2009 Subject: [Histonet] problem eyes In-Reply-To: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Message-ID: Doesn't help with the wrinkles in OLD large Histotechs. Sorry, couldn't resist Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Thursday, 5 March 2009 8:23 AM To: histonet@lists.utsouthwestern.edu; MargaretPerry; rjbuesa@yahoo.com Subject: Re: [Histonet] problem eyes Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of?liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > ren? J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help!? We have been trying to cut whole eyes > from a pig.? They need to be > nice enough for a publication.? We are having the > devil of a time because no > matter what we do there are wrinkles.? If we turn the waterbath up to > stretch things out the retina detaches.? Do any of you have > suggestions?? We uses > Surgipath? EM400 for embedding paraffin and and their > infiltration medium.? Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From stevegeagle <@t> yahoo.com Wed Mar 4 20:34:38 2009 From: stevegeagle <@t> yahoo.com (Steve Eagle) Date: Wed Mar 4 20:34:43 2009 Subject: [Histonet] colon cancer lymph nodes Message-ID: <820129.73519.qm@web36201.mail.mud.yahoo.com> An additional step that often is helpful is to submit pericolonic adipose tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid aggregates. From tifei <@t> foxmail.com Wed Mar 4 20:42:45 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Mar 4 20:42:57 2009 Subject: [Histonet] AXOn staining Message-ID: <200903051042396493204@foxmail.com> Re: Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think Tuj1 is restricted to immature neurons inside the brain ! though it stains well with retinal ganglion cell.... I am also seeking for a nice marker for axon ...both normal and regenerating...GAP-43 is one choice for regenerating axons...but a bit focused on the growth cone, and the staining is not strong on transporting proteins - due to the tiny amount? I am not sure how NF-200 or NF-96/160 work.... ANyone can suggest better markers for axon IHC staining? both normal and regenerating. _______________________________________________________________________________________________ Axons stain nicely with beta 3 tubuline (TUJ). We've used the following antibodies Monoclonal anti-beta-tubulin isotype III from Sigma 1/1000 DAKO envision (révélation PAL or HRP) (ref : T8660) or Polyclonal (rabbit) antibody against neuronal class III beta-tubulin from covance research commercialise par BABCO Berkeley antibody company au 1/1000 DAKO envision (révélation PAL or HRP) (ref : PRB-435P) We used paraffin slices of PFA fixed rat embryos and adult rat brain, microwaving in citrate buffer. These antibodies also work nicely with IF Good luck! Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Labége Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179 fax :(33)561004001 2009-03-05 TF From Kim.Osullivan <@t> med.monash.edu.au Wed Mar 4 23:17:47 2009 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Wed Mar 4 23:17:58 2009 Subject: [Histonet] Natural Killer Cell antibody Message-ID: <130.194.114.97.1236230110@my.monash.edu.au> Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim From annigyg <@t> gmail.com Thu Mar 5 00:15:35 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Mar 5 00:15:40 2009 Subject: [Histonet] problem eyes In-Reply-To: References: Message-ID: do a search on the histonet archive i posted a method quite a few years back processed and cut eyes (human and piggy) on a daily basis for almost 10 years go find it - it may help patience, determination, thicker sections, 2 x pre-float out first on dist water and then very dilute alcohol to manually tease out the crinkles, then a gentle float on low ttemp water bath if its too hot and the sections are too thin they will shatter on the warm water plenty of wax support in the block (use a bigger mould when you embed) i am on vacation and saw this post by chance unable to reply in too much detail good luck Annie (visiting in Africa) 2009/3/4 Perry, Margaret > Please help! We have been trying to cut whole eyes from a pig. They need > to be nice enough for a publication. We are having the devil of a time > because no matter what we do there are wrinkles. If we turn the waterbath > up to stretch things out the retina detaches. Do any of you have > suggestions? We uses Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is this to soft? The lowest we can turn our waterbath > is 38 degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From Susan.Walzer <@t> HCAHealthcare.com Thu Mar 5 02:19:19 2009 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Thu Mar 5 02:19:29 2009 Subject: [Histonet] RE: We are buying a new Tissue Processor and need imput In-Reply-To: <0D4634A987B07A4192E6F0F20D89B48301DBF341@ahcmascdc016.DS.SJHS.COM> References: <0D4634A987B07A4192E6F0F20D89B48301DBF341@ahcmascdc016.DS.SJHS.COM> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AABBFA8CF@FWDCWPMSGCMS09.hca.corpad.net> After 30 years in histology I say go with VIP for reliable processing. (never had one fail or damage tissue) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farnham, Lori Sent: Wednesday, March 04, 2009 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We are buying a new Tissue Processor and need imput Hi All, We are a small Pathology Lab which processes a little over 7000 surgical cases a year. We have a mixed variety of specimens (GI, GYN, skins, etc). We have been approved to buy a new tissue processor and are looking at the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these processors would be greatly appreciated. Thanks! Lori Ann Farnham, B.S.CT(ASCP) Cytotechnologist St. Mary's Hospital at Amsterdam Phone 518-841-7296 e-mail farnhaml@smha.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From iskaliora <@t> bioacademy.gr Thu Mar 5 04:17:32 2009 From: iskaliora <@t> bioacademy.gr (iskaliora) Date: Thu Mar 5 04:17:39 2009 Subject: [Histonet] staining brain vessels Message-ID: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr> I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini ----------------------------------------------------- Irini Skaliora, PhD Investigator C? (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr From pritchm <@t> ccf.org Thu Mar 5 07:00:49 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Thu Mar 5 07:01:06 2009 Subject: [Histonet] Natural Killer Cell antibody In-Reply-To: <130.194.114.97.1236230110@my.monash.edu.au> Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021513C3@CCHSCLEXMB56.cc.ad.cchs.net> CD56 (NCAM1) and CD16 (Fc gamma receptor III) are used to define human NK cell populations. Subsets of NK cells are distinguished from one another based on 'bright' or 'dim' expression of these two markers. Refer to Blood Rev. 20(3):123-37 (2006) for further information. ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim O'Sullivan Sent: Thursday, March 05, 2009 12:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Natural Killer Cell antibody Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From b-frederick <@t> northwestern.edu Thu Mar 5 07:13:15 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 5 07:13:29 2009 Subject: [Histonet] problem eyes In-Reply-To: <644825.64523.qm@web65712.mail.ac4.yahoo.com> Message-ID: We use it for fatty samples (we do lots of breast) and yes, it does work well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 04, 2009 3:28 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry; Va Paula Sicurello Subject: Re: [Histonet] problem eyes Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Ren? J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of?liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > ren? J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help!? We have been trying to cut whole eyes > from a pig.? They need to be > nice enough for a publication.? We are having the > devil of a time because no > matter what we do there are wrinkles.? If we turn the > waterbath up to stretch > things out the retina detaches.? Do any of you have > suggestions?? We uses > Surgipath? EM400 for embedding paraffin and and their > infiltration medium.? Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Thu Mar 5 07:31:28 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Mar 5 07:31:48 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gc3RhaW5pbmcgYnJhaW4gdmVzc2Vscw==?= References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr> Message-ID: <200903052131230910571@foxmail.com> hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF ???? iskaliora ????? 2009-03-05 18:49:11 ???? histonet ??? ????? ????????? ??? [Histonet] staining brain vessels I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini ----------------------------------------------------- Irini Skaliora, PhD Investigator C? (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Thu Mar 5 08:01:51 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Mar 5 08:02:12 2009 Subject: [Histonet] RE: vector blue- xylene soluble- any alternatives Message-ID: Hi Patsy and Anjan Kumar, VectaMount is not aqueous, but an organic mountant too. It does not contain alcohol or xylene, in fact it hardly smells. It certainly does not mix with water. We use this mountant by washing the slide with distilled water and then let it dry completely at a 50C hotplate. Coverslip and leave it at the hotplate for an hour or so to let it harden a bit. Vectamount is not getting hard like DPX. Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 4 Mar 2009 14:38:57 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] vector blue- xylene soluble- any alternatives To: "'anjan kumar'" , If I am not mistaken vectamount is an aqueous mountant, dpx is xylene/toluene based and requires going thru alcohols and xylene to mount. One thing I do and you can try it, is airdry the slides and the use permanent mounting media without going thru alcohols and xylene, keep in mind there is a little solvent in the media so it may have an effect. I do this with Fast Red substrate chromogen for AP have not tried it on vector blue. The other option is to use an aqueous mountant and seal the coverslip edges with clear nail polish. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, March 04, 2009 12:05 PM To: histonet-requests@lists.utsouthwestern.edu; triple immunohistochem Subject: [Histonet] vector blue- xylene soluble- any alternatives hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. From anh2006 <@t> med.cornell.edu Thu Mar 5 08:15:48 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Mar 5 08:18:28 2009 Subject: [Histonet] staining brain vessels Message-ID: <1744868416-1236262716-cardhu_decombobulator_blackberry.rim.net-330497295-@bxe1028.bisx.prod.on.blackberry> You could use CD31 but would need to digest first as it doesn't work well in fixed tissue without enzymatic retrieval. CD34 is a gem for brain vessels in paraffin so might work in fixed frozen as well. Andrea ------Original Message------ From: iskaliora Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Cc: ????? ????????? Sent: Mar 5, 2009 5:17 AM Subject: [Histonet] staining brain vessels I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini ----------------------------------------------------- Irini Skaliora, PhD Investigator C? (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vgrover <@t> polysciences.com Thu Mar 5 08:28:12 2009 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Thu Mar 5 08:31:46 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 7 Message-ID: <404F628A573A4ACB97718BC69B2F183A@USWARD13ZFB71> Hartmann's fixative: Hello All, I first became aware of this fixative in when still doing my internship from histology school at Compunet Clinical laboratory in Dayton, OH. I was asked by my technical director, Alice L. Trent to make it up from scratch, even through it is called Hartmann's Fixitive it is Davidson's modification. I also learned about it when studying intestinal biopsies and colon resections at another OSH in Toledo in 1997. Not only does tissue especially some of the fat surrounding the colon looked amazingly better than with 10% NBF, it lakes RBCs, penetrates pretty rapidly, and causes much less tissue shrinkage than 10% NBF or the mercuric fixatives (such as B-5). It is also better showing tumor patterns :nodularity in lymphoma, stromal patterns in soft-tissue and mucinous lesions. It was referred to as lymph node revealing solution because, it causes DNA-rich tissue to differentially turn white quickly. Colon malignancies fixed in Hartmann's fixative become visually as well as touch sensitive when you smash the fat you're your finger to discover smaller lymph nodes (grossly...this is a sub-grossing technique) when concerning the depth of penetration and a "deepest-penetrated" block selected. This is a valuable tool in node dissections, where 1-3 mm nodes can be found by smashing the fat with your fingers even while wearing gloves. The nodes become palpably distinct from fat and turn white (lymph node revealing solution) and as seen in a cross section on an H and E slide) We also used it a couple of times at PH for a colon CA case for one of the chief residents at the time asked me a question if I have ever heard about this fixative and he needed it to find the rest of the lymph nodes in one of his colon CA for the 1:00 pm daily conference. He has also tried it for multifocal cancer in a mastectomy case, because of some literature he followed up on from my article that was saved from my early histology education. Another formulation includes diethyl ether is used for axillary lymph node dissections for breast cancer. Small bronchi richly surrounded by chronic inflammatory cells are white on cross-section. Hartmann's is a gentler fixative, than the B-5 containing fixatives as well as 10% formalin. . Formula: 10% NBF - 24.3% 95% ETOH -32.4% acetic acid, glacial tech grade- 10.9% deionized water-32.4% of a solution totaling 100% you can adjust the volume of liquid to make 100%, so you can make 100 ml or 20 liters using these ratios. References: Loren R, et. al., "Lymph node revealing solution: A new method for detection of minute axillary lymph nodes in breast cancer specimens." Am J Surg Pathol. 1997; 21(11):1387-1390. ( this is the article given to me by my technical director Alice Louise Trent MLT(ASCP), HT(ASCP) at Compunet Clinical Labs in Dayton Ohio during my Histology School internship) Many thanks Alice!!!!!! Sincerely, Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-323-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. staining for elastic fiber of artery in rat lung (Amy Lee) 2. vector blue- xylene soluble- any alternatives (anjan kumar) 3. (no subject) (Matthew Bingham) 4. problem eyes (Perry, Margaret) 5. Re: problem eyes (Rene J Buesa) 6. Re: problem eyes (Va Paula Sicurello) 7. Re: problem eyes (Rene J Buesa) 8. Re: problem eyes (Merced Leiker) 9. Water bubble on slides (Vanessa J. Phelan) 10. RE: problem eyes (Connolly, Brett M) 11. RE: vector blue- xylene soluble- any alternatives (Patsy Ruegg) 12. Re: Water bubble on slides (Merced Leiker) 13. Autotechnicon model 2A (Va Paula Sicurello) 14. RE: problem eyes (Tony Henwood) 15. colon cancer lymph nodes (Steve Eagle) 16. AXOn staining (TF) 17. Natural Killer Cell antibody (Kim O'Sullivan) 18. Re: problem eyes (Anne van Binsbergen) 19. RE: We are buying a new Tissue Processor and need imput (Walzer Susan) 20. staining brain vessels (iskaliora) 21. RE: Natural Killer Cell antibody (Pritchard, Michele) 22. RE: problem eyes (Bernice Frederick) 23. Re: staining brain vessels (TF) ---------------------------------------------------------------------- Message: 1 Date: Wed, 4 Mar 2009 10:21:43 -0800 (PST) From: Amy Lee Subject: [Histonet] staining for elastic fiber of artery in rat lung To: histonet Message-ID: <937896.88292.qm@web38006.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello histonetters, Would you teach me what is best stain for elastic fiber in rat lung except weigert's stain? I want to look at arteries. Thanks in advance, Weihua ------------------------------ Message: 2 Date: Thu, 5 Mar 2009 00:34:32 +0530 From: anjan kumar Subject: [Histonet] vector blue- xylene soluble- any alternatives To: , triple immunohistochem Message-ID: Content-Type: text/plain; charset="iso-8859-1" hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag ------------------------------ Message: 3 Date: Wed, 4 Mar 2009 11:43:28 -0800 (PST) From: Matthew Bingham Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <322914.55412.qm@web63507.mail.re1.yahoo.com> Content-Type: text/plain; charset=us-ascii As many of you may or may not know, the Histobath countertop freezing bath has been discontinued by Thermo (formerly known as Thermo-Shandon, Shandon-Lipshaw, etc.). I was told that the freon that is used to allow the unit to cool to -60 is no longer allowed by the EPA to be used in ultra low refrigeration units. I know there is a floor model, the Clini-RF, at brightinstruments.com which uses a two types of freon in isolated side by side systems, but it is a rather large unit (20"D x 18"W x 40"H). Our frozen section room, as are most, is a small room with very limited space. Therefore, have any of you found a solution for a countertop model (i.e. 19"D x 9"W x 15"H)freezing bath to be used in frozen section rooms? I appreciate the help. Thanks, Matthew Bingham, PA(ASCP)CMAnatomic Pathology Supervisor Phoenix Children's Hospital Department of Pathology 1919 E. Thomas Road Phoenix, AZ 85016 phone: 602-546-1318 fax: 602-546-1284 email: mbingham@phoenixchildrens.com ------------------------------ Message: 4 Date: Wed, 4 Mar 2009 15:04:23 -0600 From: "Perry, Margaret" Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ------------------------------ Message: 5 Date: Wed, 4 Mar 2009 13:14:56 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" Message-ID: <240791.54814.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). reni J. --- On Wed, 3/4/09, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 4 Mar 2009 13:23:18 -0800 (PST) From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , MargaretPerry , rjbuesa@yahoo.com Message-ID: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 4 Mar 2009 13:27:32 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , MargaretPerry , Va Paula Sicurello Message-ID: <644825.64523.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Reni J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Wed, 04 Mar 2009 16:29:12 -0500 From: Merced Leiker Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu Message-ID: <7FD71F250513F2B17D70DB05@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed Good question! I'd like to know that, too! But with any size specimen, please! --On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello wrote: > > Really? Liquid laundry soap? Does this help with all wrinkles in large > specimens? > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Microscope Facility, room B141 > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Wed, 3/4/09, Rene J Buesa wrote: > >> From: Rene J Buesa >> Subject: Re: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> , "Perry, Margaret" >> Date: Wednesday, March 4, 2009, 9:14 PM >> Add a few drops (4-5) of liquid >> detergent, but not dishwasher detergent (because it will >> dissolve the paraffin). >> reni J. >> >> --- On Wed, 3/4/09, Perry, Margaret >> wrote: >> >> From: Perry, Margaret >> Subject: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> >> Date: Wednesday, March 4, 2009, 4:04 PM >> >> Please help! We have been trying to cut whole eyes >> from a pig. They need to be >> nice enough for a publication. We are having the >> devil of a time because no >> matter what we do there are wrinkles. If we turn the >> waterbath up to stretch >> things out the retina detaches. Do any of you have >> suggestions? We uses >> Surgipath EM400 for embedding paraffin and and their >> infiltration medium. Is >> this to soft? The lowest we can turn our waterbath is 38 >> degrees C. >> >> Margaret Perry HT (ASCP) >> IHC Lab Manager Veterinary Science >> Animal Disease Research and Diagnostic Lab >> South Dakota State University >> Box 2175 North Campus Drive >> Brookings SD 57007 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 9 Date: Wed, 4 Mar 2009 16:35:14 -0500 From: "Vanessa J. Phelan" Subject: [Histonet] Water bubble on slides To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi everyone, Quick question, I am finding after cutting a lot of slides for H&Es onto VWR Superfrost Plus slides (these are the only slides we have at the moment) that when I lift sections from the water bath ( of distilled water) a water bubble stays under the sections on the slide. When I lie them flat on the hot plate to dry the water bubble is tending to distort the tissue by escaping out through he tissue, eventually. I have not experienced this before, the water has ran off the slide no problem. Would it be the slides that are the problem or anything I might need to add to the water? I have just ordered a histology oven, this may be the solution...drying the slides standing up! Vanessa ------------------------------ Message: 10 Date: Wed, 4 Mar 2009 16:36:19 -0500 From: "Connolly, Brett M" Subject: RE: [Histonet] problem eyes To: "Perry, Margaret" , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201C38A0B@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" My suggestion would be to follow the procedure of the old ocular guru from the AFIP, Peter Emanuale. He taught me how the section human and rabbit eyes. It involves using 2 water baths, one of which is at room temp... and you should not use charged/coated slides. Check our his chapter on ocular histotechnology in the 1992 edition of the AFIP Lab Methods manual (it has step by step illustrations) Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, March 04, 2009 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem eyes Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 11 Date: Wed, 4 Mar 2009 14:38:57 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] vector blue- xylene soluble- any alternatives To: "'anjan kumar'" , , "'triple immunohistochem'" Message-ID: <011DFB4829B140CE8DF5E5222EAE7497@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" If I am not mistaken vectamount is an aqueous mountant, dpx is xylene/toluene based and requires going thru alcohols and xylene to mount. One thing I do and you can try it, is airdry the slides and the use permanent mounting media without going thru alcohols and xylene, keep in mind there is a little solvent in the media so it may have an effect. I do this with Fast Red substrate chromogen for AP have not tried it on vector blue. The other option is to use an aqueous mountant and seal the coverslip edges with clear nail polish. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, March 04, 2009 12:05 PM To: histonet-requests@lists.utsouthwestern.edu; triple immunohistochem Subject: [Histonet] vector blue- xylene soluble- any alternatives hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag______________________ _________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 04 Mar 2009 16:50:29 -0500 From: Merced Leiker Subject: Re: [Histonet] Water bubble on slides To: "Vanessa J. Phelan" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi Vanessa, I have read in at least one online protocol and have also been advised by our excellent and experienced (former) histotech who used to be here at UB NOT to warm the slides without air-drying first. We typically air-dry the slides, yes, standing up, overnight, before warming them. Merced --On Wednesday, March 04, 2009 4:35 PM -0500 "Vanessa J. Phelan" wrote: > Hi everyone, > > Quick question, I am finding after cutting a lot of slides for H&Es onto > VWR Superfrost Plus slides (these are the only slides we have at the > moment) that when I lift sections from the water bath ( of distilled > water) a water bubble stays under the sections on the slide. When I lie > them flat on the hot plate to dry the water bubble is tending to distort > the tissue by escaping out through he tissue, eventually. I have not > experienced this before, the water has ran off the slide no problem. > Would it be the slides that are the problem or anything I might need to > add to the water? > > I have just ordered a histology oven, this may be the solution...drying > the slides standing up! > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 13 Date: Wed, 4 Mar 2009 14:39:33 -0800 (PST) From: Va Paula Sicurello Subject: [Histonet] Autotechnicon model 2A To: HistoNet Message-ID: <969177.76764.qm@web46110.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi All, Does anybody have an owner's manual to an Autotechnicon 2A? I've inherited this 42 year old beasty and can't remember how to work the delay timer. Yes, I am old enough to have used one of these R2D2 units in the past. If you can help me, I'd be much obliged. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ------------------------------ Message: 14 Date: Thu, 5 Mar 2009 09:41:39 +1100 From: "Tony Henwood" Subject: RE: [Histonet] problem eyes To: "Va Paula Sicurello" , , "MargaretPerry" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Doesn't help with the wrinkles in OLD large Histotechs. Sorry, couldn't resist Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Thursday, 5 March 2009 8:23 AM To: histonet@lists.utsouthwestern.edu; MargaretPerry; rjbuesa@yahoo.com Subject: Re: [Histonet] problem eyes Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the waterbath up to > stretch things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 15 Date: Wed, 4 Mar 2009 18:34:38 -0800 (PST) From: Steve Eagle Subject: [Histonet] colon cancer lymph nodes To: histonet@lists.utsouthwestern.edu Message-ID: <820129.73519.qm@web36201.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii An additional step that often is helpful is to submit pericolonic adipose tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid aggregates. ------------------------------ Message: 16 Date: Thu, 5 Mar 2009 10:42:45 +0800 From: "TF" Subject: [Histonet] AXOn staining To: "nancy.walker" Cc: histonet Message-ID: <200903051042396493204@foxmail.com> Content-Type: text/plain; charset="us-ascii" Re: Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think Tuj1 is restricted to immature neurons inside the brain ! though it stains well with retinal ganglion cell.... I am also seeking for a nice marker for axon ...both normal and regenerating...GAP-43 is one choice for regenerating axons...but a bit focused on the growth cone, and the staining is not strong on transporting proteins - due to the tiny amount? I am not sure how NF-200 or NF-96/160 work.... ANyone can suggest better markers for axon IHC staining? both normal and regenerating. ____________________________________________________________________________ ___________________ Axons stain nicely with beta 3 tubuline (TUJ). We've used the following antibodies Monoclonal anti-beta-tubulin isotype III from Sigma 1/1000 DAKO envision (rivilation PAL or HRP) (ref : T8660) or Polyclonal (rabbit) antibody against neuronal class III beta-tubulin from covance research commercialise par BABCO Berkeley antibody company au 1/1000 DAKO envision (rivilation PAL or HRP) (ref : PRB-435P) We used paraffin slices of PFA fixed rat embryos and adult rat brain, microwaving in citrate buffer. These antibodies also work nicely with IF Good luck! Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Labige Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179 fax :(33)561004001 2009-03-05 TF ------------------------------ Message: 17 Date: Thu, 05 Mar 2009 16:17:47 +1100 From: Kim O'Sullivan Subject: [Histonet] Natural Killer Cell antibody To: histonet@lists.utsouthwestern.edu Message-ID: <130.194.114.97.1236230110@my.monash.edu.au> Content-Type: text/plain; charset=UTF-8 Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim ------------------------------ Message: 18 Date: Thu, 5 Mar 2009 08:15:35 +0200 From: Anne van Binsbergen Subject: Re: [Histonet] problem eyes To: "Perry, Margaret" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 do a search on the histonet archive i posted a method quite a few years back processed and cut eyes (human and piggy) on a daily basis for almost 10 years go find it - it may help patience, determination, thicker sections, 2 x pre-float out first on dist water and then very dilute alcohol to manually tease out the crinkles, then a gentle float on low ttemp water bath if its too hot and the sections are too thin they will shatter on the warm water plenty of wax support in the block (use a bigger mould when you embed) i am on vacation and saw this post by chance unable to reply in too much detail good luck Annie (visiting in Africa) 2009/3/4 Perry, Margaret > Please help! We have been trying to cut whole eyes from a pig. They need > to be nice enough for a publication. We are having the devil of a time > because no matter what we do there are wrinkles. If we turn the waterbath > up to stretch things out the retina detaches. Do any of you have > suggestions? We uses Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is this to soft? The lowest we can turn our waterbath > is 38 degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 19 Date: Thu, 5 Mar 2009 02:19:19 -0600 From: Walzer Susan Subject: [Histonet] RE: We are buying a new Tissue Processor and need imput To: "Farnham, Lori" , "histonet@lists.utsouthwestern.edu" Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AABBFA8CF@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" After 30 years in histology I say go with VIP for reliable processing. (never had one fail or damage tissue) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farnham, Lori Sent: Wednesday, March 04, 2009 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We are buying a new Tissue Processor and need imput Hi All, We are a small Pathology Lab which processes a little over 7000 surgical cases a year. We have a mixed variety of specimens (GI, GYN, skins, etc). We have been approved to buy a new tissue processor and are looking at the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these processors would be greatly appreciated. Thanks! Lori Ann Farnham, B.S.CT(ASCP) Cytotechnologist St. Mary's Hospital at Amsterdam Phone 518-841-7296 e-mail farnhaml@smha.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 5 Mar 2009 12:17:32 +0200 From: iskaliora Subject: [Histonet] staining brain vessels To: histonet@lists.utsouthwestern.edu Cc: ????? ????????? Message-ID: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr> Content-Type: text/plain; charset=UTF-8; format=flowed; delsp=yes I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini ----------------------------------------------------- Irini Skaliora, PhD Investigator Ca>= (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr ------------------------------ Message: 21 Date: Thu, 5 Mar 2009 08:00:49 -0500 From: "Pritchard, Michele" Subject: RE: [Histonet] Natural Killer Cell antibody To: "Kim O'Sullivan" , histonet@lists.utsouthwestern.edu Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021513C3@CCHSCLEXMB56.cc.ad.cchs.net> Content-Type: text/plain; charset=us-ascii CD56 (NCAM1) and CD16 (Fc gamma receptor III) are used to define human NK cell populations. Subsets of NK cells are distinguished from one another based on 'bright' or 'dim' expression of these two markers. Refer to Blood Rev. 20(3):123-37 (2006) for further information. ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim O'Sullivan Sent: Thursday, March 05, 2009 12:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Natural Killer Cell antibody Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ------------------------------ Message: 22 Date: Thu, 5 Mar 2009 07:13:15 -0600 From: "Bernice Frederick" Subject: RE: [Histonet] problem eyes To: , , "'MargaretPerry'" , "'Va Paula Sicurello'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use it for fatty samples (we do lots of breast) and yes, it does work well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 04, 2009 3:28 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry; Va Paula Sicurello Subject: Re: [Histonet] problem eyes Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Reni J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Thu, 5 Mar 2009 21:31:28 +0800 From: "TF" Subject: Re: [Histonet] staining brain vessels To: "iskaliora" , "histonet" Cc: ????? ????????? Message-ID: <200903052131230910571@foxmail.com> Content-Type: text/plain; charset="utf-8" hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF ed;6d::o< iskaliora ei From vgrover <@t> polysciences.com Thu Mar 5 08:40:24 2009 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Thu Mar 5 08:43:58 2009 Subject: [Histonet] "Re: Contents of Histonet digest..." Message-ID: <366246721F1C425AB5B1F5B811018557@USWARD13ZFB71> Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-323-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: Valantou Grover [mailto:vgrover@polysciences.com] Sent: Thursday, March 05, 2009 9:28 AM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: Histonet Digest, Vol 64, Issue 7 Importance: High Hartmann's fixative: Hello All, I first became aware of this fixative in when still doing my internship from histology school at Compunet Clinical laboratory in Dayton, OH. I was asked by my technical director, Alice L. Trent to make it up from scratch, even through it is called Hartmann's Fixitive it is Davidson's modification. I also learned about it when studying intestinal biopsies and colon resections at another OSH in Toledo in 1997. Not only does tissue especially some of the fat surrounding the colon looked amazingly better than with 10% NBF, it lakes RBCs, penetrates pretty rapidly, and causes much less tissue shrinkage than 10% NBF or the mercuric fixatives (such as B-5). It is also better showing tumor patterns :nodularity in lymphoma, stromal patterns in soft-tissue and mucinous lesions. It was referred to as lymph node revealing solution because, it causes DNA-rich tissue to differentially turn white quickly. Colon malignancies fixed in Hartmann's fixative become visually as well as touch sensitive when you smash the fat you're your finger to discover smaller lymph nodes (grossly...this is a sub-grossing technique) when concerning the depth of penetration and a "deepest-penetrated" block selected. This is a valuable tool in node dissections, where 1-3 mm nodes can be found by smashing the fat with your fingers even while wearing gloves. The nodes become palpably distinct from fat and turn white (lymph node revealing solution) and as seen in a cross section on an H and E slide) We also used it a couple of times at PH for a colon CA case for one of the chief residents at the time asked me a question if I have ever heard about this fixative and he needed it to find the rest of the lymph nodes in one of his colon CA for the 1:00 pm daily conference. He has also tried it for multifocal cancer in a mastectomy case, because of some literature he followed up on from my article that was saved from my early histology education. Another formulation includes diethyl ether is used for axillary lymph node dissections for breast cancer. Small bronchi richly surrounded by chronic inflammatory cells are white on cross-section. Hartmann's is a gentler fixative, than the B-5 containing fixatives as well as 10% formalin. . Formula: 10% NBF - 24.3% 95% ETOH -32.4% acetic acid, glacial tech grade- 10.9% deionized water-32.4% of a solution totaling 100% you can adjust the volume of liquid to make 100%, so you can make 100 ml or 20 liters using these ratios. References: Loren R, et. al., "Lymph node revealing solution: A new method for detection of minute axillary lymph nodes in breast cancer specimens." Am J Surg Pathol. 1997; 21(11):1387-1390. ( this is the article given to me by my technical director Alice Louise Trent MLT(ASCP), HT(ASCP) at Compunet Clinical Labs in Dayton Ohio during my Histology School internship) Many thanks Alice!!!!!! Sincerely, Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-323-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. staining for elastic fiber of artery in rat lung (Amy Lee) 2. vector blue- xylene soluble- any alternatives (anjan kumar) 3. (no subject) (Matthew Bingham) 4. problem eyes (Perry, Margaret) 5. Re: problem eyes (Rene J Buesa) 6. Re: problem eyes (Va Paula Sicurello) 7. Re: problem eyes (Rene J Buesa) 8. Re: problem eyes (Merced Leiker) 9. Water bubble on slides (Vanessa J. Phelan) 10. RE: problem eyes (Connolly, Brett M) 11. RE: vector blue- xylene soluble- any alternatives (Patsy Ruegg) 12. Re: Water bubble on slides (Merced Leiker) 13. Autotechnicon model 2A (Va Paula Sicurello) 14. RE: problem eyes (Tony Henwood) 15. colon cancer lymph nodes (Steve Eagle) 16. AXOn staining (TF) 17. Natural Killer Cell antibody (Kim O'Sullivan) 18. Re: problem eyes (Anne van Binsbergen) 19. RE: We are buying a new Tissue Processor and need imput (Walzer Susan) 20. staining brain vessels (iskaliora) 21. RE: Natural Killer Cell antibody (Pritchard, Michele) 22. RE: problem eyes (Bernice Frederick) 23. Re: staining brain vessels (TF) ---------------------------------------------------------------------- Message: 1 Date: Wed, 4 Mar 2009 10:21:43 -0800 (PST) From: Amy Lee Subject: [Histonet] staining for elastic fiber of artery in rat lung To: histonet Message-ID: <937896.88292.qm@web38006.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello histonetters, Would you teach me what is best stain for elastic fiber in rat lung except weigert's stain? I want to look at arteries. Thanks in advance, Weihua ------------------------------ Message: 2 Date: Thu, 5 Mar 2009 00:34:32 +0530 From: anjan kumar Subject: [Histonet] vector blue- xylene soluble- any alternatives To: , triple immunohistochem Message-ID: Content-Type: text/plain; charset="iso-8859-1" hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag ------------------------------ Message: 3 Date: Wed, 4 Mar 2009 11:43:28 -0800 (PST) From: Matthew Bingham Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <322914.55412.qm@web63507.mail.re1.yahoo.com> Content-Type: text/plain; charset=us-ascii As many of you may or may not know, the Histobath countertop freezing bath has been discontinued by Thermo (formerly known as Thermo-Shandon, Shandon-Lipshaw, etc.). I was told that the freon that is used to allow the unit to cool to -60 is no longer allowed by the EPA to be used in ultra low refrigeration units. I know there is a floor model, the Clini-RF, at brightinstruments.com which uses a two types of freon in isolated side by side systems, but it is a rather large unit (20"D x 18"W x 40"H). Our frozen section room, as are most, is a small room with very limited space. Therefore, have any of you found a solution for a countertop model (i.e. 19"D x 9"W x 15"H)freezing bath to be used in frozen section rooms? I appreciate the help. Thanks, Matthew Bingham, PA(ASCP)CMAnatomic Pathology Supervisor Phoenix Children's Hospital Department of Pathology 1919 E. Thomas Road Phoenix, AZ 85016 phone: 602-546-1318 fax: 602-546-1284 email: mbingham@phoenixchildrens.com ------------------------------ Message: 4 Date: Wed, 4 Mar 2009 15:04:23 -0600 From: "Perry, Margaret" Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ------------------------------ Message: 5 Date: Wed, 4 Mar 2009 13:14:56 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" Message-ID: <240791.54814.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). reni J. --- On Wed, 3/4/09, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 4 Mar 2009 13:23:18 -0800 (PST) From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , MargaretPerry , rjbuesa@yahoo.com Message-ID: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 4 Mar 2009 13:27:32 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , MargaretPerry , Va Paula Sicurello Message-ID: <644825.64523.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Reni J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Wed, 04 Mar 2009 16:29:12 -0500 From: Merced Leiker Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu Message-ID: <7FD71F250513F2B17D70DB05@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed Good question! I'd like to know that, too! But with any size specimen, please! --On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello wrote: > > Really? Liquid laundry soap? Does this help with all wrinkles in large > specimens? > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Microscope Facility, room B141 > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Wed, 3/4/09, Rene J Buesa wrote: > >> From: Rene J Buesa >> Subject: Re: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> , "Perry, Margaret" >> Date: Wednesday, March 4, 2009, 9:14 PM >> Add a few drops (4-5) of liquid >> detergent, but not dishwasher detergent (because it will >> dissolve the paraffin). >> reni J. >> >> --- On Wed, 3/4/09, Perry, Margaret >> wrote: >> >> From: Perry, Margaret >> Subject: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> >> Date: Wednesday, March 4, 2009, 4:04 PM >> >> Please help! We have been trying to cut whole eyes >> from a pig. They need to be >> nice enough for a publication. We are having the >> devil of a time because no >> matter what we do there are wrinkles. If we turn the >> waterbath up to stretch >> things out the retina detaches. Do any of you have >> suggestions? We uses >> Surgipath EM400 for embedding paraffin and and their >> infiltration medium. Is >> this to soft? The lowest we can turn our waterbath is 38 >> degrees C. >> >> Margaret Perry HT (ASCP) >> IHC Lab Manager Veterinary Science >> Animal Disease Research and Diagnostic Lab >> South Dakota State University >> Box 2175 North Campus Drive >> Brookings SD 57007 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 9 Date: Wed, 4 Mar 2009 16:35:14 -0500 From: "Vanessa J. Phelan" Subject: [Histonet] Water bubble on slides To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi everyone, Quick question, I am finding after cutting a lot of slides for H&Es onto VWR Superfrost Plus slides (these are the only slides we have at the moment) that when I lift sections from the water bath ( of distilled water) a water bubble stays under the sections on the slide. When I lie them flat on the hot plate to dry the water bubble is tending to distort the tissue by escaping out through he tissue, eventually. I have not experienced this before, the water has ran off the slide no problem. Would it be the slides that are the problem or anything I might need to add to the water? I have just ordered a histology oven, this may be the solution...drying the slides standing up! Vanessa ------------------------------ Message: 10 Date: Wed, 4 Mar 2009 16:36:19 -0500 From: "Connolly, Brett M" Subject: RE: [Histonet] problem eyes To: "Perry, Margaret" , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201C38A0B@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" My suggestion would be to follow the procedure of the old ocular guru from the AFIP, Peter Emanuale. He taught me how the section human and rabbit eyes. It involves using 2 water baths, one of which is at room temp... and you should not use charged/coated slides. Check our his chapter on ocular histotechnology in the 1992 edition of the AFIP Lab Methods manual (it has step by step illustrations) Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, March 04, 2009 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem eyes Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 11 Date: Wed, 4 Mar 2009 14:38:57 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] vector blue- xylene soluble- any alternatives To: "'anjan kumar'" , , "'triple immunohistochem'" Message-ID: <011DFB4829B140CE8DF5E5222EAE7497@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" If I am not mistaken vectamount is an aqueous mountant, dpx is xylene/toluene based and requires going thru alcohols and xylene to mount. One thing I do and you can try it, is airdry the slides and the use permanent mounting media without going thru alcohols and xylene, keep in mind there is a little solvent in the media so it may have an effect. I do this with Fast Red substrate chromogen for AP have not tried it on vector blue. The other option is to use an aqueous mountant and seal the coverslip edges with clear nail polish. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, March 04, 2009 12:05 PM To: histonet-requests@lists.utsouthwestern.edu; triple immunohistochem Subject: [Histonet] vector blue- xylene soluble- any alternatives hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag______________________ _________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 04 Mar 2009 16:50:29 -0500 From: Merced Leiker Subject: Re: [Histonet] Water bubble on slides To: "Vanessa J. Phelan" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi Vanessa, I have read in at least one online protocol and have also been advised by our excellent and experienced (former) histotech who used to be here at UB NOT to warm the slides without air-drying first. We typically air-dry the slides, yes, standing up, overnight, before warming them. Merced --On Wednesday, March 04, 2009 4:35 PM -0500 "Vanessa J. Phelan" wrote: > Hi everyone, > > Quick question, I am finding after cutting a lot of slides for H&Es onto > VWR Superfrost Plus slides (these are the only slides we have at the > moment) that when I lift sections from the water bath ( of distilled > water) a water bubble stays under the sections on the slide. When I lie > them flat on the hot plate to dry the water bubble is tending to distort > the tissue by escaping out through he tissue, eventually. I have not > experienced this before, the water has ran off the slide no problem. > Would it be the slides that are the problem or anything I might need to > add to the water? > > I have just ordered a histology oven, this may be the solution...drying > the slides standing up! > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 13 Date: Wed, 4 Mar 2009 14:39:33 -0800 (PST) From: Va Paula Sicurello Subject: [Histonet] Autotechnicon model 2A To: HistoNet Message-ID: <969177.76764.qm@web46110.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi All, Does anybody have an owner's manual to an Autotechnicon 2A? I've inherited this 42 year old beasty and can't remember how to work the delay timer. Yes, I am old enough to have used one of these R2D2 units in the past. If you can help me, I'd be much obliged. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ------------------------------ Message: 14 Date: Thu, 5 Mar 2009 09:41:39 +1100 From: "Tony Henwood" Subject: RE: [Histonet] problem eyes To: "Va Paula Sicurello" , , "MargaretPerry" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Doesn't help with the wrinkles in OLD large Histotechs. Sorry, couldn't resist Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Thursday, 5 March 2009 8:23 AM To: histonet@lists.utsouthwestern.edu; MargaretPerry; rjbuesa@yahoo.com Subject: Re: [Histonet] problem eyes Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the waterbath up to > stretch things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 15 Date: Wed, 4 Mar 2009 18:34:38 -0800 (PST) From: Steve Eagle Subject: [Histonet] colon cancer lymph nodes To: histonet@lists.utsouthwestern.edu Message-ID: <820129.73519.qm@web36201.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii An additional step that often is helpful is to submit pericolonic adipose tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid aggregates. ------------------------------ Message: 16 Date: Thu, 5 Mar 2009 10:42:45 +0800 From: "TF" Subject: [Histonet] AXOn staining To: "nancy.walker" Cc: histonet Message-ID: <200903051042396493204@foxmail.com> Content-Type: text/plain; charset="us-ascii" Re: Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think Tuj1 is restricted to immature neurons inside the brain ! though it stains well with retinal ganglion cell.... I am also seeking for a nice marker for axon ...both normal and regenerating...GAP-43 is one choice for regenerating axons...but a bit focused on the growth cone, and the staining is not strong on transporting proteins - due to the tiny amount? I am not sure how NF-200 or NF-96/160 work.... ANyone can suggest better markers for axon IHC staining? both normal and regenerating. ____________________________________________________________________________ ___________________ Axons stain nicely with beta 3 tubuline (TUJ). We've used the following antibodies Monoclonal anti-beta-tubulin isotype III from Sigma 1/1000 DAKO envision (rivilation PAL or HRP) (ref : T8660) or Polyclonal (rabbit) antibody against neuronal class III beta-tubulin from covance research commercialise par BABCO Berkeley antibody company au 1/1000 DAKO envision (rivilation PAL or HRP) (ref : PRB-435P) We used paraffin slices of PFA fixed rat embryos and adult rat brain, microwaving in citrate buffer. These antibodies also work nicely with IF Good luck! Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Labige Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179 fax :(33)561004001 2009-03-05 TF ------------------------------ Message: 17 Date: Thu, 05 Mar 2009 16:17:47 +1100 From: Kim O'Sullivan Subject: [Histonet] Natural Killer Cell antibody To: histonet@lists.utsouthwestern.edu Message-ID: <130.194.114.97.1236230110@my.monash.edu.au> Content-Type: text/plain; charset=UTF-8 Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim ------------------------------ Message: 18 Date: Thu, 5 Mar 2009 08:15:35 +0200 From: Anne van Binsbergen Subject: Re: [Histonet] problem eyes To: "Perry, Margaret" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 do a search on the histonet archive i posted a method quite a few years back processed and cut eyes (human and piggy) on a daily basis for almost 10 years go find it - it may help patience, determination, thicker sections, 2 x pre-float out first on dist water and then very dilute alcohol to manually tease out the crinkles, then a gentle float on low ttemp water bath if its too hot and the sections are too thin they will shatter on the warm water plenty of wax support in the block (use a bigger mould when you embed) i am on vacation and saw this post by chance unable to reply in too much detail good luck Annie (visiting in Africa) 2009/3/4 Perry, Margaret > Please help! We have been trying to cut whole eyes from a pig. They need > to be nice enough for a publication. We are having the devil of a time > because no matter what we do there are wrinkles. If we turn the waterbath > up to stretch things out the retina detaches. Do any of you have > suggestions? We uses Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is this to soft? The lowest we can turn our waterbath > is 38 degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 19 Date: Thu, 5 Mar 2009 02:19:19 -0600 From: Walzer Susan Subject: [Histonet] RE: We are buying a new Tissue Processor and need imput To: "Farnham, Lori" , "histonet@lists.utsouthwestern.edu" Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AABBFA8CF@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" After 30 years in histology I say go with VIP for reliable processing. (never had one fail or damage tissue) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farnham, Lori Sent: Wednesday, March 04, 2009 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We are buying a new Tissue Processor and need imput Hi All, We are a small Pathology Lab which processes a little over 7000 surgical cases a year. We have a mixed variety of specimens (GI, GYN, skins, etc). We have been approved to buy a new tissue processor and are looking at the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these processors would be greatly appreciated. Thanks! Lori Ann Farnham, B.S.CT(ASCP) Cytotechnologist St. Mary's Hospital at Amsterdam Phone 518-841-7296 e-mail farnhaml@smha.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 5 Mar 2009 12:17:32 +0200 From: iskaliora Subject: [Histonet] staining brain vessels To: histonet@lists.utsouthwestern.edu Cc: ????? ????????? Message-ID: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr> Content-Type: text/plain; charset=UTF-8; format=flowed; delsp=yes I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini ----------------------------------------------------- Irini Skaliora, PhD Investigator Ca>= (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr ------------------------------ Message: 21 Date: Thu, 5 Mar 2009 08:00:49 -0500 From: "Pritchard, Michele" Subject: RE: [Histonet] Natural Killer Cell antibody To: "Kim O'Sullivan" , histonet@lists.utsouthwestern.edu Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021513C3@CCHSCLEXMB56.cc.ad.cchs.net> Content-Type: text/plain; charset=us-ascii CD56 (NCAM1) and CD16 (Fc gamma receptor III) are used to define human NK cell populations. Subsets of NK cells are distinguished from one another based on 'bright' or 'dim' expression of these two markers. Refer to Blood Rev. 20(3):123-37 (2006) for further information. ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim O'Sullivan Sent: Thursday, March 05, 2009 12:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Natural Killer Cell antibody Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ------------------------------ Message: 22 Date: Thu, 5 Mar 2009 07:13:15 -0600 From: "Bernice Frederick" Subject: RE: [Histonet] problem eyes To: , , "'MargaretPerry'" , "'Va Paula Sicurello'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use it for fatty samples (we do lots of breast) and yes, it does work well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 04, 2009 3:28 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry; Va Paula Sicurello Subject: Re: [Histonet] problem eyes Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Reni J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Thu, 5 Mar 2009 21:31:28 +0800 From: "TF" Subject: Re: [Histonet] staining brain vessels To: "iskaliora" , "histonet" Cc: ????? ????????? Message-ID: <200903052131230910571@foxmail.com> Content-Type: text/plain; charset="utf-8" hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF ed;6d::o< iskaliora ei From lblazek <@t> digestivespecialists.com Thu Mar 5 08:48:16 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 5 08:44:36 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 7 In-Reply-To: <404F628A573A4ACB97718BC69B2F183A@USWARD13ZFB71> References: <404F628A573A4ACB97718BC69B2F183A@USWARD13ZFB71> Message-ID: <5A2BD13465E061429D6455C8D6B40E390870C5D6C1@IBMB7Exchange.digestivespecialists.com> Alice says you're welcome! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Valantou Grover Sent: Thursday, March 05, 2009 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 7 Importance: High Hartmann's fixative: Hello All, I first became aware of this fixative in when still doing my internship from histology school at Compunet Clinical laboratory in Dayton, OH. I was asked by my technical director, Alice L. Trent to make it up from scratch, even through it is called Hartmann's Fixitive it is Davidson's modification. I also learned about it when studying intestinal biopsies and colon resections at another OSH in Toledo in 1997. Not only does tissue especially some of the fat surrounding the colon looked amazingly better than with 10% NBF, it lakes RBCs, penetrates pretty rapidly, and causes much less tissue shrinkage than 10% NBF or the mercuric fixatives (such as B-5). It is also better showing tumor patterns :nodularity in lymphoma, stromal patterns in soft-tissue and mucinous lesions. It was referred to as lymph node revealing solution because, it causes DNA-rich tissue to differentially turn white quickly. Colon malignancies fixed in Hartmann's fixative become visually as well as touch sensitive when you smash the fat you're your finger to discover smaller lymph nodes (grossly...this is a sub-grossing technique) when concerning the depth of penetration and a "deepest-penetrated" block selected. This is a valuable tool in node dissections, where 1-3 mm nodes can be found by smashing the fat with your fingers even while wearing gloves. The nodes become palpably distinct from fat and turn white (lymph node revealing solution) and as seen in a cross section on an H and E slide) We also used it a couple of times at PH for a colon CA case for one of the chief residents at the time asked me a question if I have ever heard about this fixative and he needed it to find the rest of the lymph nodes in one of his colon CA for the 1:00 pm daily conference. He has also tried it for multifocal cancer in a mastectomy case, because of some literature he followed up on from my article that was saved from my early histology education. Another formulation includes diethyl ether is used for axillary lymph node dissections for breast cancer. Small bronchi richly surrounded by chronic inflammatory cells are white on cross-section. Hartmann's is a gentler fixative, than the B-5 containing fixatives as well as 10% formalin. . Formula: 10% NBF - 24.3% 95% ETOH -32.4% acetic acid, glacial tech grade- 10.9% deionized water-32.4% of a solution totaling 100% you can adjust the volume of liquid to make 100%, so you can make 100 ml or 20 liters using these ratios. References: Loren R, et. al., "Lymph node revealing solution: A new method for detection of minute axillary lymph nodes in breast cancer specimens." Am J Surg Pathol. 1997; 21(11):1387-1390. ( this is the article given to me by my technical director Alice Louise Trent MLT(ASCP), HT(ASCP) at Compunet Clinical Labs in Dayton Ohio during my Histology School internship) Many thanks Alice!!!!!! Sincerely, Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-323-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. staining for elastic fiber of artery in rat lung (Amy Lee) 2. vector blue- xylene soluble- any alternatives (anjan kumar) 3. (no subject) (Matthew Bingham) 4. problem eyes (Perry, Margaret) 5. Re: problem eyes (Rene J Buesa) 6. Re: problem eyes (Va Paula Sicurello) 7. Re: problem eyes (Rene J Buesa) 8. Re: problem eyes (Merced Leiker) 9. Water bubble on slides (Vanessa J. Phelan) 10. RE: problem eyes (Connolly, Brett M) 11. RE: vector blue- xylene soluble- any alternatives (Patsy Ruegg) 12. Re: Water bubble on slides (Merced Leiker) 13. Autotechnicon model 2A (Va Paula Sicurello) 14. RE: problem eyes (Tony Henwood) 15. colon cancer lymph nodes (Steve Eagle) 16. AXOn staining (TF) 17. Natural Killer Cell antibody (Kim O'Sullivan) 18. Re: problem eyes (Anne van Binsbergen) 19. RE: We are buying a new Tissue Processor and need imput (Walzer Susan) 20. staining brain vessels (iskaliora) 21. RE: Natural Killer Cell antibody (Pritchard, Michele) 22. RE: problem eyes (Bernice Frederick) 23. Re: staining brain vessels (TF) ---------------------------------------------------------------------- Message: 1 Date: Wed, 4 Mar 2009 10:21:43 -0800 (PST) From: Amy Lee Subject: [Histonet] staining for elastic fiber of artery in rat lung To: histonet Message-ID: <937896.88292.qm@web38006.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello histonetters, Would you teach me what is best stain for elastic fiber in rat lung except weigert's stain? I want to look at arteries. Thanks in advance, Weihua ------------------------------ Message: 2 Date: Thu, 5 Mar 2009 00:34:32 +0530 From: anjan kumar Subject: [Histonet] vector blue- xylene soluble- any alternatives To: , triple immunohistochem Message-ID: Content-Type: text/plain; charset="iso-8859-1" hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag ------------------------------ Message: 3 Date: Wed, 4 Mar 2009 11:43:28 -0800 (PST) From: Matthew Bingham Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <322914.55412.qm@web63507.mail.re1.yahoo.com> Content-Type: text/plain; charset=us-ascii As many of you may or may not know, the Histobath countertop freezing bath has been discontinued by Thermo (formerly known as Thermo-Shandon, Shandon-Lipshaw, etc.). I was told that the freon that is used to allow the unit to cool to -60 is no longer allowed by the EPA to be used in ultra low refrigeration units. I know there is a floor model, the Clini-RF, at brightinstruments.com which uses a two types of freon in isolated side by side systems, but it is a rather large unit (20"D x 18"W x 40"H). Our frozen section room, as are most, is a small room with very limited space. Therefore, have any of you found a solution for a countertop model (i.e. 19"D x 9"W x 15"H)freezing bath to be used in frozen section rooms? I appreciate the help. Thanks, Matthew Bingham, PA(ASCP)CMAnatomic Pathology Supervisor Phoenix Children's Hospital Department of Pathology 1919 E. Thomas Road Phoenix, AZ 85016 phone: 602-546-1318 fax: 602-546-1284 email: mbingham@phoenixchildrens.com ------------------------------ Message: 4 Date: Wed, 4 Mar 2009 15:04:23 -0600 From: "Perry, Margaret" Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ------------------------------ Message: 5 Date: Wed, 4 Mar 2009 13:14:56 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" Message-ID: <240791.54814.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). reni J. --- On Wed, 3/4/09, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 4 Mar 2009 13:23:18 -0800 (PST) From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , MargaretPerry , rjbuesa@yahoo.com Message-ID: <787849.53788.qm@web46101.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 4 Mar 2009 13:27:32 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , MargaretPerry , Va Paula Sicurello Message-ID: <644825.64523.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Reni J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Wed, 04 Mar 2009 16:29:12 -0500 From: Merced Leiker Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu Message-ID: <7FD71F250513F2B17D70DB05@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed Good question! I'd like to know that, too! But with any size specimen, please! --On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello wrote: > > Really? Liquid laundry soap? Does this help with all wrinkles in large > specimens? > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Microscope Facility, room B141 > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Wed, 3/4/09, Rene J Buesa wrote: > >> From: Rene J Buesa >> Subject: Re: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> , "Perry, Margaret" >> Date: Wednesday, March 4, 2009, 9:14 PM >> Add a few drops (4-5) of liquid >> detergent, but not dishwasher detergent (because it will >> dissolve the paraffin). >> reni J. >> >> --- On Wed, 3/4/09, Perry, Margaret >> wrote: >> >> From: Perry, Margaret >> Subject: [Histonet] problem eyes >> To: "histonet@lists.utsouthwestern.edu" >> >> Date: Wednesday, March 4, 2009, 4:04 PM >> >> Please help! We have been trying to cut whole eyes >> from a pig. They need to be >> nice enough for a publication. We are having the >> devil of a time because no >> matter what we do there are wrinkles. If we turn the >> waterbath up to stretch >> things out the retina detaches. Do any of you have >> suggestions? We uses >> Surgipath EM400 for embedding paraffin and and their >> infiltration medium. Is >> this to soft? The lowest we can turn our waterbath is 38 >> degrees C. >> >> Margaret Perry HT (ASCP) >> IHC Lab Manager Veterinary Science >> Animal Disease Research and Diagnostic Lab >> South Dakota State University >> Box 2175 North Campus Drive >> Brookings SD 57007 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 9 Date: Wed, 4 Mar 2009 16:35:14 -0500 From: "Vanessa J. Phelan" Subject: [Histonet] Water bubble on slides To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi everyone, Quick question, I am finding after cutting a lot of slides for H&Es onto VWR Superfrost Plus slides (these are the only slides we have at the moment) that when I lift sections from the water bath ( of distilled water) a water bubble stays under the sections on the slide. When I lie them flat on the hot plate to dry the water bubble is tending to distort the tissue by escaping out through he tissue, eventually. I have not experienced this before, the water has ran off the slide no problem. Would it be the slides that are the problem or anything I might need to add to the water? I have just ordered a histology oven, this may be the solution...drying the slides standing up! Vanessa ------------------------------ Message: 10 Date: Wed, 4 Mar 2009 16:36:19 -0500 From: "Connolly, Brett M" Subject: RE: [Histonet] problem eyes To: "Perry, Margaret" , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201C38A0B@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" My suggestion would be to follow the procedure of the old ocular guru from the AFIP, Peter Emanuale. He taught me how the section human and rabbit eyes. It involves using 2 water baths, one of which is at room temp... and you should not use charged/coated slides. Check our his chapter on ocular histotechnology in the 1992 edition of the AFIP Lab Methods manual (it has step by step illustrations) Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, March 04, 2009 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem eyes Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 11 Date: Wed, 4 Mar 2009 14:38:57 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] vector blue- xylene soluble- any alternatives To: "'anjan kumar'" , , "'triple immunohistochem'" Message-ID: <011DFB4829B140CE8DF5E5222EAE7497@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" If I am not mistaken vectamount is an aqueous mountant, dpx is xylene/toluene based and requires going thru alcohols and xylene to mount. One thing I do and you can try it, is airdry the slides and the use permanent mounting media without going thru alcohols and xylene, keep in mind there is a little solvent in the media so it may have an effect. I do this with Fast Red substrate chromogen for AP have not tried it on vector blue. The other option is to use an aqueous mountant and seal the coverslip edges with clear nail polish. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, March 04, 2009 12:05 PM To: histonet-requests@lists.utsouthwestern.edu; triple immunohistochem Subject: [Histonet] vector blue- xylene soluble- any alternatives hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag______________________ _________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 04 Mar 2009 16:50:29 -0500 From: Merced Leiker Subject: Re: [Histonet] Water bubble on slides To: "Vanessa J. Phelan" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi Vanessa, I have read in at least one online protocol and have also been advised by our excellent and experienced (former) histotech who used to be here at UB NOT to warm the slides without air-drying first. We typically air-dry the slides, yes, standing up, overnight, before warming them. Merced --On Wednesday, March 04, 2009 4:35 PM -0500 "Vanessa J. Phelan" wrote: > Hi everyone, > > Quick question, I am finding after cutting a lot of slides for H&Es onto > VWR Superfrost Plus slides (these are the only slides we have at the > moment) that when I lift sections from the water bath ( of distilled > water) a water bubble stays under the sections on the slide. When I lie > them flat on the hot plate to dry the water bubble is tending to distort > the tissue by escaping out through he tissue, eventually. I have not > experienced this before, the water has ran off the slide no problem. > Would it be the slides that are the problem or anything I might need to > add to the water? > > I have just ordered a histology oven, this may be the solution...drying > the slides standing up! > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 13 Date: Wed, 4 Mar 2009 14:39:33 -0800 (PST) From: Va Paula Sicurello Subject: [Histonet] Autotechnicon model 2A To: HistoNet Message-ID: <969177.76764.qm@web46110.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi All, Does anybody have an owner's manual to an Autotechnicon 2A? I've inherited this 42 year old beasty and can't remember how to work the delay timer. Yes, I am old enough to have used one of these R2D2 units in the past. If you can help me, I'd be much obliged. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ------------------------------ Message: 14 Date: Thu, 5 Mar 2009 09:41:39 +1100 From: "Tony Henwood" Subject: RE: [Histonet] problem eyes To: "Va Paula Sicurello" , , "MargaretPerry" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Doesn't help with the wrinkles in OLD large Histotechs. Sorry, couldn't resist Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Thursday, 5 March 2009 8:23 AM To: histonet@lists.utsouthwestern.edu; MargaretPerry; rjbuesa@yahoo.com Subject: Re: [Histonet] problem eyes Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the waterbath up to > stretch things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 15 Date: Wed, 4 Mar 2009 18:34:38 -0800 (PST) From: Steve Eagle Subject: [Histonet] colon cancer lymph nodes To: histonet@lists.utsouthwestern.edu Message-ID: <820129.73519.qm@web36201.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii An additional step that often is helpful is to submit pericolonic adipose tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid aggregates. ------------------------------ Message: 16 Date: Thu, 5 Mar 2009 10:42:45 +0800 From: "TF" Subject: [Histonet] AXOn staining To: "nancy.walker" Cc: histonet Message-ID: <200903051042396493204@foxmail.com> Content-Type: text/plain; charset="us-ascii" Re: Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think Tuj1 is restricted to immature neurons inside the brain ! though it stains well with retinal ganglion cell.... I am also seeking for a nice marker for axon ...both normal and regenerating...GAP-43 is one choice for regenerating axons...but a bit focused on the growth cone, and the staining is not strong on transporting proteins - due to the tiny amount? I am not sure how NF-200 or NF-96/160 work.... ANyone can suggest better markers for axon IHC staining? both normal and regenerating. ____________________________________________________________________________ ___________________ Axons stain nicely with beta 3 tubuline (TUJ). We've used the following antibodies Monoclonal anti-beta-tubulin isotype III from Sigma 1/1000 DAKO envision (rivilation PAL or HRP) (ref : T8660) or Polyclonal (rabbit) antibody against neuronal class III beta-tubulin from covance research commercialise par BABCO Berkeley antibody company au 1/1000 DAKO envision (rivilation PAL or HRP) (ref : PRB-435P) We used paraffin slices of PFA fixed rat embryos and adult rat brain, microwaving in citrate buffer. These antibodies also work nicely with IF Good luck! Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Labige Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179 fax :(33)561004001 2009-03-05 TF ------------------------------ Message: 17 Date: Thu, 05 Mar 2009 16:17:47 +1100 From: Kim O'Sullivan Subject: [Histonet] Natural Killer Cell antibody To: histonet@lists.utsouthwestern.edu Message-ID: <130.194.114.97.1236230110@my.monash.edu.au> Content-Type: text/plain; charset=UTF-8 Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim ------------------------------ Message: 18 Date: Thu, 5 Mar 2009 08:15:35 +0200 From: Anne van Binsbergen Subject: Re: [Histonet] problem eyes To: "Perry, Margaret" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 do a search on the histonet archive i posted a method quite a few years back processed and cut eyes (human and piggy) on a daily basis for almost 10 years go find it - it may help patience, determination, thicker sections, 2 x pre-float out first on dist water and then very dilute alcohol to manually tease out the crinkles, then a gentle float on low ttemp water bath if its too hot and the sections are too thin they will shatter on the warm water plenty of wax support in the block (use a bigger mould when you embed) i am on vacation and saw this post by chance unable to reply in too much detail good luck Annie (visiting in Africa) 2009/3/4 Perry, Margaret > Please help! We have been trying to cut whole eyes from a pig. They need > to be nice enough for a publication. We are having the devil of a time > because no matter what we do there are wrinkles. If we turn the waterbath > up to stretch things out the retina detaches. Do any of you have > suggestions? We uses Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is this to soft? The lowest we can turn our waterbath > is 38 degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 19 Date: Thu, 5 Mar 2009 02:19:19 -0600 From: Walzer Susan Subject: [Histonet] RE: We are buying a new Tissue Processor and need imput To: "Farnham, Lori" , "histonet@lists.utsouthwestern.edu" Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AABBFA8CF@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" After 30 years in histology I say go with VIP for reliable processing. (never had one fail or damage tissue) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farnham, Lori Sent: Wednesday, March 04, 2009 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We are buying a new Tissue Processor and need imput Hi All, We are a small Pathology Lab which processes a little over 7000 surgical cases a year. We have a mixed variety of specimens (GI, GYN, skins, etc). We have been approved to buy a new tissue processor and are looking at the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these processors would be greatly appreciated. Thanks! Lori Ann Farnham, B.S.CT(ASCP) Cytotechnologist St. Mary's Hospital at Amsterdam Phone 518-841-7296 e-mail farnhaml@smha.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 5 Mar 2009 12:17:32 +0200 From: iskaliora Subject: [Histonet] staining brain vessels To: histonet@lists.utsouthwestern.edu Cc: ????? ????????? Message-ID: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr> Content-Type: text/plain; charset=UTF-8; format=flowed; delsp=yes I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini ----------------------------------------------------- Irini Skaliora, PhD Investigator Ca>= (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskaliora@bioacademy.gr ------------------------------ Message: 21 Date: Thu, 5 Mar 2009 08:00:49 -0500 From: "Pritchard, Michele" Subject: RE: [Histonet] Natural Killer Cell antibody To: "Kim O'Sullivan" , histonet@lists.utsouthwestern.edu Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021513C3@CCHSCLEXMB56.cc.ad.cchs.net> Content-Type: text/plain; charset=us-ascii CD56 (NCAM1) and CD16 (Fc gamma receptor III) are used to define human NK cell populations. Subsets of NK cells are distinguished from one another based on 'bright' or 'dim' expression of these two markers. Refer to Blood Rev. 20(3):123-37 (2006) for further information. ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim O'Sullivan Sent: Thursday, March 05, 2009 12:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Natural Killer Cell antibody Hi, Does anyone out there know if there is a monoclonal anti human NK cell antibody that is exclusive, ie does not also mark CD8 T cells etc? Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ------------------------------ Message: 22 Date: Thu, 5 Mar 2009 07:13:15 -0600 From: "Bernice Frederick" Subject: RE: [Histonet] problem eyes To: , , "'MargaretPerry'" , "'Va Paula Sicurello'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use it for fatty samples (we do lots of breast) and yes, it does work well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 04, 2009 3:28 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry; Va Paula Sicurello Subject: Re: [Histonet] problem eyes Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. Reni J. --- On Wed, 3/4/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: Re: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" , "MargaretPerry" , rjbuesa@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa wrote: > From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. > > --- On Wed, 3/4/09, Perry, Margaret > wrote: > > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn the > waterbath up to stretch > things out the retina detaches. Do any of you have > suggestions? We uses > Surgipath EM400 for embedding paraffin and and their > infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 > degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Thu, 5 Mar 2009 21:31:28 +0800 From: "TF" Subject: Re: [Histonet] staining brain vessels To: "iskaliora" , "histonet" Cc: ????? ????????? Message-ID: <200903052131230910571@foxmail.com> Content-Type: text/plain; charset="utf-8" hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF ed;6d::o< iskaliora ei _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Thu Mar 5 09:07:20 2009 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Mar 5 09:07:44 2009 Subject: [Histonet] Full Mount Immunos Message-ID: Does anyone know of any companies producing charged full mount slides? I have a pathologist that wants immunos done on full mount prostates and the tissue keeps falling off. I have looked, but cannot find any charged full mount slides. Any help would be appreciated!! Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From maxim_71 <@t> mail.ru Thu Mar 5 09:12:08 2009 From: maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Mar 5 09:12:42 2009 Subject: [Histonet] problem eyes Message-ID: <997903449.20090305181208@mail.ru> We also addes 4-5 drops of Tween 20 in waterbath. Human eye's sections have not any wrinlkes. Maxim Peshkov, Russia, Taganrog. --- On Wed, 3/4/09, Perry, Margaret > From: Perry, Margaret > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn > the waterbath up to stretch > things out the retina detaches. Do any of you have suggestions? We uses > Surgipath EM400 for embedding paraffin and and > their infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007. From algranth <@t> u.arizona.edu Thu Mar 5 09:19:53 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Mar 5 09:19:05 2009 Subject: [Histonet] gelatin beads Message-ID: <6.2.3.4.1.20090305080304.02723330@algranth.inbox.email.arizona.edu> Good Morning, I have an investigator who is bringing me rat brains that have been injected with gelatin beads. I'm sectioning through the injection site(s) and she is hoping to see the beads. BUT even though the injection sites are easy to see there are no gelatin beads present. My question is, are they being washed out somehow by fixation/processing or dropping out in the waterbath as she is telling me? Or are they melting in the paraffin steps (60 degrees)? If either of these are the case I'm thinking that frozen sections might be the solution? Sorry I don't have more info on the beads. I'll try to get that today. Thanks, Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From rjbuesa <@t> yahoo.com Thu Mar 5 09:20:31 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 5 09:20:34 2009 Subject: [Histonet] Full Mount Immunos In-Reply-To: Message-ID: <301714.88872.qm@web65715.mail.ac4.yahoo.com> You will have to silanize your slides. Ren? J. --- On Thu, 3/5/09, Metzger, Kenneth wrote: From: Metzger, Kenneth Subject: [Histonet] Full Mount Immunos To: histonet@lists.utsouthwestern.edu Date: Thursday, March 5, 2009, 10:07 AM Does anyone know of any companies producing charged full mount slides? I have a pathologist that wants immunos done on full mount prostates and the tissue keeps falling off. I have looked, but cannot find any charged full mount slides. Any help would be appreciated!! Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Thu Mar 5 09:23:02 2009 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Mar 5 09:23:05 2009 Subject: [Histonet] RE: Full Mount Immunos In-Reply-To: References: Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D3168ED6F4FD@JHEMTEXVS3.win.ad.jhu.edu> I believe that Brain Research Labs has large "+" slides. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Thursday, March 05, 2009 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Full Mount Immunos Does anyone know of any companies producing charged full mount slides? I have a pathologist that wants immunos done on full mount prostates and the tissue keeps falling off. I have looked, but cannot find any charged full mount slides. Any help would be appreciated!! Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 5 09:27:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 5 09:27:19 2009 Subject: [Histonet] gelatin beads In-Reply-To: <6.2.3.4.1.20090305080304.02723330@algranth.inbox.email.arizona.edu> Message-ID: <609231.77574.qm@web65703.mail.ac4.yahoo.com> The gelatin beads are water soluble and will dissolve in any water containing step. Also, as you point out, they will melt at high temperature. Your solution of doing FS?is probably the best way of trying to look for these beads. Ren? J. --- On Thu, 3/5/09, Andrea Grantham wrote: From: Andrea Grantham Subject: [Histonet] gelatin beads To: histonet@lists.utsouthwestern.edu Date: Thursday, March 5, 2009, 10:19 AM Good Morning, I have an investigator who is bringing me rat brains that have been injected with gelatin beads. I'm sectioning through the injection site(s) and she is hoping to see the beads. BUT even though the injection sites are easy to see there are no gelatin beads present. My question is, are they being washed out somehow by fixation/processing or dropping out in the waterbath as she is telling me? Or are they melting in the paraffin steps (60 degrees)? If either of these are the case I'm thinking that frozen sections might be the solution? Sorry I don't have more info on the beads. I'll try to get that today. Thanks, Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Mar 5 10:04:42 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Mar 5 10:04:53 2009 Subject: [Histonet] aqueous mounting media Message-ID: <62A8156F8071C8439080D626DF8C33A65D8B7A@wave-mail.7thwave.local> Can anyone recommend a good aqueous mounting media for coverslipping Oil Red O stained slides? We are currently using Dako's Faramount media, but have been having problems with excessive air bubbles; especially, it seems, in the tissues containing a lot of fat. I recently heard that Dako's Glycergel is better for this type of application. Any suggestions are greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From jrobertson <@t> pathologysciences.com Thu Mar 5 10:15:12 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Thu Mar 5 10:15:16 2009 Subject: [Histonet] aqueous mounting media In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B7A@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A65D8B7A@wave-mail.7thwave.local> Message-ID: <518CD6920AA7154193CBE5977CD88073177EDE@psmgsrv2.PSMG.local> Faramount by Dako works well for us. Jodie Robertson, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, March 05, 2009 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting media Can anyone recommend a good aqueous mounting media for coverslipping Oil Red O stained slides? We are currently using Dako's Faramount media, but have been having problems with excessive air bubbles; especially, it seems, in the tissues containing a lot of fat. I recently heard that Dako's Glycergel is better for this type of application. Any suggestions are greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Mar 5 10:17:39 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Mar 5 10:17:42 2009 Subject: [Histonet] aqueous mounting media In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B7A@wave-mail.7thwave.local> Message-ID: I use Aqua-Mount. I have never had a problem. It is from Lerner Laboratories but I think you can get it from any major vendor. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, March 05, 2009 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting media Can anyone recommend a good aqueous mounting media for coverslipping Oil Red O stained slides? We are currently using Dako's Faramount media, but have been having problems with excessive air bubbles; especially, it seems, in the tissues containing a lot of fat. I recently heard that Dako's Glycergel is better for this type of application. Any suggestions are greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Mar 5 10:51:53 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 5 10:52:06 2009 Subject: AW: [Histonet] staining for elastic fiber of artery in rat lung In-Reply-To: <937896.88292.qm@web38006.mail.mud.yahoo.com> References: <937896.88292.qm@web38006.mail.mud.yahoo.com> Message-ID: <2AEF5AE1D8934AAA88A8FD9734C45346@dielangs.at> Do you know or mean the Verhoeff Elastin stain? Verhoeff staining solution: 25 ml 10% Haematoxylin in abs. ethanol 25 ml abs. ethanol 25 ml 10% FeCl3 (CAS 7705080) Mix well and ad 25 ml Verhoeff iodinsolution (=2 g Iodine + 4 g KI + 100 ml water) 1. deparaffinize and rehydrate slides 2. Verhoeff staining solution 20 min 3. lukewarme tapwater 5 min 4. differentiate in 2% FeCl3 1-2 min 5. rinse in Aqua dest. 6. rinse in 5% Sodiumthiosulfate 1 min 7. rinse in running tapwater 8. counterstain as you like 9. dehydrate, clear and coverslip Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amy Lee Gesendet: Mittwoch, 04. M?rz 2009 19:22 An: histonet Betreff: [Histonet] staining for elastic fiber of artery in rat lung Hello histonetters, ? Would you teach me what is best stain for elastic fiber in rat lung except weigert's stain? ? I want to look at arteries. ? Thanks in advance, Weihua _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Thu Mar 5 10:57:24 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Mar 5 10:57:29 2009 Subject: [Histonet] staining brain vessels In-Reply-To: <200903052131230910571@foxmail.com> References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr> <200903052131230910571@foxmail.com> Message-ID: <49B00474.2070909@umn.edu> You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > ???? iskaliora > ????? 2009-03-05 18:49:11 > ???? histonet > ??? ????? ????????? > ??? [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cforster <@t> umn.edu Thu Mar 5 10:58:16 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Mar 5 10:58:22 2009 Subject: [Histonet] staining brain vessels In-Reply-To: <1744868416-1236262716-cardhu_decombobulator_blackberry.rim.net-330497295-@bxe1028.bisx.prod.on.blackberry> References: <1744868416-1236262716-cardhu_decombobulator_blackberry.rim.net-330497295-@bxe1028.bisx.prod.on.blackberry> Message-ID: <49B004A8.1090508@umn.edu> Again, you need to be sure that it will work in mouse samples. CD34 is another marker that does not work well in mouse. Colleen Forster anh2006@med.cornell.edu wrote: > You could use CD31 but would need to digest first as it doesn't work well in fixed tissue without enzymatic retrieval. > > CD34 is a gem for brain vessels in paraffin so might work in fixed frozen as well. > > Andrea > > ------Original Message------ > From: iskaliora > Sender: histonet-bounces@lists.utsouthwestern.edu > To: histonet@lists.utsouthwestern.edu > Cc: ????? ????????? > Sent: Mar 5, 2009 5:17 AM > Subject: [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > > > > > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tifei <@t> foxmail.com Thu Mar 5 11:27:53 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Mar 5 11:28:12 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIHN0YWluaW5nIGJyYWluIHZlc3NlbHM=?= References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr>, <200903052131230910571@foxmail.com>, <49B00474.2070909@umn.edu> Message-ID: <200903060127478503624@foxmail.com> Anyone know the Abcam CD31 on rat brain? http://www.abcam.com/CD31-antibody-ab28364.html Or suggest another one? 2009-03-06 TF ???? Colleen Forster ????? 2009-03-06 00:57:29 ???? tifei ??? iskaliora; histonet; ????? ????????? ??? Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > ???? iskaliora > ????? 2009-03-05 18:49:11 > ???? histonet > ??? ????? ????????? > ??? [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anh2006 <@t> med.cornell.edu Thu Mar 5 11:41:54 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Mar 5 11:42:09 2009 Subject: [Histonet] staining brain vessels In-Reply-To: <49B004A8.1090508@umn.edu> References: <"1744868416-1236262716-cardhu_decombobulator_blackberry.rim.net-330497295 -"@bxe1028.bisx.prod.on.blackberry> <49B004A8.1090508@umn.edu> Message-ID: Hi Colleen, Although I agree that you have to make sure your markers are good for the particular species you work with, I respectfully disagree that CD34 does not "work" in mouse. CD34 is one of the best vascular markers for mouse paraffin sections, of course it is absolutely tissue specific. In mouse brain, specifically, it is the best pan-EC marker I have encountered. Thanks, Andrea At 10:58 AM -0600 3/5/09, Colleen Forster wrote: >Again, you need to be sure that it will work in >mouse samples. CD34 is another marker that does >not work well in mouse. > >Colleen Forster > > >anh2006@med.cornell.edu wrote: >>You could use CD31 but would need to digest >>first as it doesn't work well in fixed tissue >>without enzymatic retrieval. >> >>CD34 is a gem for brain vessels in paraffin so >>might work in fixed frozen as well. >>Andrea >> >>------Original Message------ >>From: iskaliora >>Sender: histonet-bounces@lists.utsouthwestern.edu >>To: histonet@lists.utsouthwestern.edu >>Cc: ?????????* ?????????????????* >>Sent: Mar 5, 2009 5:17 AM >>Subject: [Histonet] staining brain vessels >> >>I was wondering if anybody might have an idea >>with the following problem we are >>experiencing: we want to stain for blood >>vessels in sections of mouse brain. Our >>experimental tissues have been fixed overnight >>in 4% paraformaldehyde and have been sitting in >>PBS since. >>We have tried staining with antibodies against >>desmin, SMA, and collagen but we get NO >>specific signal. We recently tried a non-fixed >>mouse brain and got desmin to work immediately. >>The problem is that we need to use the fixed >>brains because they are our experimental model >>and it would take too long (2 years to be >>exact) to generate the same samples. If >>anybody has come across such a problem before, >>or has a specific protocol for vessels that >>works on PFA fixed brain, we would appreciate >>the suggestions! >>thanks in advance! >>Irini >> >> >> >> >>----------------------------------------------------- >>Irini Skaliora, PhD >>Investigator C? (Assistant Professor) >>Developmental Biology Division >>Biomedical Research Foundation of the Academy of Athens (BRFAA) >>Soranou Efessiou 4 >>Athens 11527 >>tel. +30-210-6597203 (office) >>tel. +30-210-6597482 (lab) >>fax. +30-210-6597545 >>email: iskaliora@bioacademy.gr >> >> >> >> -- From aevans <@t> wellspan.org Thu Mar 5 11:54:55 2009 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Thu Mar 5 11:55:47 2009 Subject: [Histonet] (no subject) Message-ID: Does anyone run a third shift in their lab to relieve some stress on the 1st shift crew?? I am looking in to running one person on third. I would like to know how it is working for you, has it reduced costs, has it increased turn around time...ect. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From dellav <@t> musc.edu Thu Mar 5 12:10:32 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Mar 5 12:10:37 2009 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: We are a 24 hour lab. Once you have a night shift I doubt you'll go back to a day only operation. Even the staff that may be resistant often don't want to go back to days. Think about it, the opportunity to do your work with no interruptions, no phone calls. You can crank up the tunes and no one will complain. It's amazing how much work can be done sans interruptions. Has it saved money? I'm confident it's prevented a boat load of overtime but we do pay a shift differential (still cheaper than OT). No more complaints from the docs that the slides are late. We also do most of our IHC staining at night so everyone's stains are ready first thing in the morning when the docs start arriving. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Thursday, March 05, 2009 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Does anyone run a third shift in their lab to relieve some stress on the 1st shift crew?? I am looking in to running one person on third. I would like to know how it is working for you, has it reduced costs, has it increased turn around time...ect. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Thu Mar 5 12:38:16 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Mar 5 12:38:21 2009 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: We run a third shift with 2 people and they do all the biopsies and stat specimens. This works great if you can keep the shift staffed. I have had to replace 5 people in 2 years. I am currently looking into getting a rapid processor so I do the biopsies during the day and totally eliminate my night shift. I would also like to add that the 3rd shift is not a very family friendly shift so it truly takes someone with special circumstances to stay with it for very long. Christie UAB Hospital Birmingham > Date: Thu, 5 Mar 2009 12:54:55 -0500 > From: aevans@wellspan.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Does anyone run a third shift in their lab to relieve some stress on the 1st shift crew?? I am looking in to running one person on third. I would like to know how it is working for you, has it reduced costs, has it increased turn around time...ect. Thanks!! > > > Andria B Evans, HTL(ASCP)CM > Anatomic Pathology > York Hospital > 1001 S. George Street > York, PA 17405 > 717-851-5006 > > "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe > > "Maturity is accepting imperfections." > > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. > > > ______________________________________________________________________ > This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsnauss <@t> bellsouth.net Thu Mar 5 12:40:20 2009 From: nsnauss <@t> bellsouth.net (Nathanial nauss) Date: Thu Mar 5 12:40:25 2009 Subject: [Histonet] VIP 6 Message-ID: <767077.59939.qm@web83905.mail.sp1.yahoo.com> We are looking at the VIP 6 and the Leica ASP300, I was wondering if any one has any experience with the VIP6. Nathaniel From mtc205 <@t> Lehigh.EDU Thu Mar 5 12:43:40 2009 From: mtc205 <@t> Lehigh.EDU (Matthew T Close) Date: Thu Mar 5 12:43:44 2009 Subject: [Histonet] re: staining of elastic fibers Message-ID: <28650700.1236278620714.JavaMail.mtc205@lehigh.edu> Weihua, I prefer iron gallein elastin stain because it is make and use and gives good contrast compared with some of t techniques. The recipes and protocol can be found in Humanson's An imal Tissue Techniques (in addition to many other histological techniques t (please email and I wil black with gallein, nucle dark blue-black. Differentiat the counterstain is your personal choi saturated picric acid is typically used with g gallein itself is hardest to find, but can currently be p from Fisher Scientific, Spectrum Chemical, or ArtChemicals.com.&nb sp; I believe that spectrum has it cheapest, and I would buy at least 25g i Sincerament Matt Close ------- Matthew T. Close Lehigh University Department of Biological Sciences From vapatpxs <@t> yahoo.com Thu Mar 5 13:09:10 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Mar 5 13:09:15 2009 Subject: [Histonet] (no subject) Message-ID: <806058.23419.qm@web46115.mail.sp1.yahoo.com> I worked for a hospital that ran 3 shifts. I worked 2:00pm to 10:30pm. Others came in at 4:00am and began the embedding process, once the people who started work at between 7:00 and 8:00am arrived the blocks were ready to go. We had one person who came in at 11:00pm and worked solely on the small samples, GI biopsies, etc. I batted clean-up and finished off anything left over from the day shift. Usually recuts, deepers and a variety of specials. I also did the histology for the research samples that caem in but they had lower priority. I also loaded all the processors and checked to make sure that the baskets were properly loaded and were in numeric order. The 11:00pm person burned out and they went back to the 3 shifts. then I left and they went back to 2 shifts, early morning and regular days. The Pathologists loved it when we had 3 shifts but when I left no one took over the clean-up shift. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Thu, 3/5/09, Evans, Andria B. wrote: > From: Evans, Andria B. > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Date: Thursday, March 5, 2009, 5:54 PM > Does anyone run a third shift in > their lab to relieve some stress on the 1st shift > crew??? I am looking in to running one person on > third.? I would like to know how it is working for you, > has it reduced costs, has it increased turn around > time...ect.? Thanks!! > > > Andria B Evans, HTL(ASCP)CM > Anatomic Pathology > York Hospital > 1001 S. George Street > York, PA? 17405 > 717-851-5006 > > "You can learn a lot more from listening than you can from > talking.? Find someone with whom you don't agree in the > slightest and ask them to explain themselves at > length.? Then take a seat, shut your mouth, and don't > argue back.? It's physically impossible to listen with > your mouth open." -John Moe > > "Maturity is accepting imperfections." > > CONFIDENTIALITY NOTICE:? > > This email may contain confidential health information that > is legally privileged.? This information is intended > for the use of the named recipient(s). The authorized > recipient of this information is prohibited from disclosing > this information to any party unless required to do so by > law or regulation and is required to destroy the information > after its stated need has been fulfilled.? If you are > not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or action taken in > reliance on the contents of this email is strictly > prohibited.? If you receive this e-mail message in > error, please notify the sender immediately to arrange > disposition of the information. > > > ______________________________________________________________________ > This e-mail has been scanned by MCI Managed Email Content > Service, using Skeptic(tm) technology powered by > MessageLabs. For more information on MCI's Managed > Email? Content Service, visit http://www.mci.com. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From micropathlabs <@t> yahoo.com Thu Mar 5 13:13:40 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Mar 5 13:13:43 2009 Subject: [Histonet] Lab Week Activities Message-ID: <207087.91225.qm@web57807.mail.re3.yahoo.com> Does anyone have any new ideas for lab week activities? We usually?do?bagels and luncheons but are looking for something different this year. This is the first year that our billing office and laboratorians are in the same building and we want to do something to bring together the masses, so to speak. Any ideas would be appreciated. Thanks in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From crochieresteve <@t> aol.com Thu Mar 5 13:41:47 2009 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Thu Mar 5 13:43:23 2009 Subject: [Histonet] Histo supervisor Job opening Message-ID: <8CB6BE18E5C7C04-EE0-1118@webmail-mf17.sysops.aol.com> We are looking for a supervisory level Histologist with experience in IHC to complement our laboratory staff. If you are interested please forward a rsume to Christine Sullivan Business manager LifePath Partners, LLC 299 Carew St. Springfield, MA 01104 413-748-9779 Christine.sullivan@sphs.com From vapatpxs <@t> yahoo.com Thu Mar 5 13:50:45 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Mar 5 13:50:55 2009 Subject: [Histonet] Thank you Message-ID: <394529.80714.qm@web46111.mail.sp1.yahoo.com> I'd like to thank everyone who tried to help me with the Autotechnicon owner's manual. A great big thanks to Augusto in Caracas, Venezuela who sent me a pdf of the owner's manual. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From jkeller <@t> seattlecca.org Thu Mar 5 15:38:43 2009 From: jkeller <@t> seattlecca.org (Keller, Jason E) Date: Thu Mar 5 15:38:48 2009 Subject: [Histonet] In need of AFB controls Message-ID: <29381_1236289124_n25Lch3x030223_4EA6CBCAB26218408EFCEC255A8862410796EA92@storm.seattlecca.org> I am in need of AFB control blocks. They would need to be from human tissue. Willing to consider a trade for other control blocks. Jason E. Keller HTL (ASCP) Seattle Cancer Care Alliance Pathology Department Lab: 206-288-1355 Desk: 206-288-1358 This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From Shakun.Aswani <@t> acologix.com Thu Mar 5 15:41:48 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Thu Mar 5 15:41:53 2009 Subject: [Histonet] In need of AFB controls In-Reply-To: <29381_1236289124_n25Lch3x030223_4EA6CBCAB26218408EFCEC255A8862410796EA92@storm.seattlecca.org> References: <29381_1236289124_n25Lch3x030223_4EA6CBCAB26218408EFCEC255A8862410796EA92@storm.seattlecca.org> Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301ADE030@EXCHANGE.acologix.com> Hi Jason, Contact CDC they might provide you Shakun -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keller, Jason E Sent: Thursday, March 05, 2009 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] In need of AFB controls I am in need of AFB control blocks. They would need to be from human tissue. Willing to consider a trade for other control blocks. Jason E. Keller HTL (ASCP) Seattle Cancer Care Alliance Pathology Department Lab: 206-288-1355 Desk: 206-288-1358 This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Mar 5 17:53:49 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Mar 5 17:53:53 2009 Subject: [Histonet] GFP antibody Message-ID: <62A8156F8071C8439080D626DF8C33A65D8B83@wave-mail.7thwave.local> Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From cforster <@t> umn.edu Thu Mar 5 17:56:41 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Mar 5 17:56:45 2009 Subject: [Histonet] GFP antibody In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B83@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A65D8B83@wave-mail.7thwave.local> Message-ID: <49B066B9.4070009@umn.edu> I am all ears to hear if anyone has gotten really good GFP signal after the FFPE process. I have not been able too. I have looked at a few articles but the images don't convince me. it seems to me that the processing squelches the GFP activity...... Michele Wich wrote: > Can anyone recommend a green fluorescent protein (GFP) antibody that > works well in FFPE murine tissue? I have never used this antibody before > and am starting from scratch, so any advice is greatly appreciated. > > > > > This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From anh2006 <@t> med.cornell.edu Thu Mar 5 18:08:18 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Mar 5 18:11:02 2009 Subject: [Histonet] GFP antibody Message-ID: <1413635187-1236298267-cardhu_decombobulator_blackberry.rim.net-361284447-@bxe1028.bisx.prod.on.blackberry> The rabbit pAb antibody from Molecular Probes invitrogen works great in frozen and fixed frozen so it might work in paraffin. Worth a shot.... Andrea ------Original Message------ From: Michele Wich Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mar 5, 2009 6:53 PM Subject: [Histonet] GFP antibody Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Thu Mar 5 19:59:54 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Mar 5 20:03:27 2009 Subject: [Histonet] Epithelial cells Message-ID: Hi all - We have a graduate student growing equine epithelial cells in culture. Money is really short. She is wondering if there is a regular special stain for these epithelial cells rather than the more expensive IHC. Any suggestions? Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From ccrowder <@t> vetmed.lsu.edu Thu Mar 5 19:59:54 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Mar 5 20:03:31 2009 Subject: [Histonet] Epithelial cells Message-ID: Hi all - We have a graduate student growing equine epithelial cells in culture. Money is really short. She is wondering if there is a regular special stain for these epithelial cells rather than the more expensive IHC. Any suggestions? Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From sshawdfy <@t> med.kobe-u.ac.jp Thu Mar 5 20:52:36 2009 From: sshawdfy <@t> med.kobe-u.ac.jp (shymaa shawadfy) Date: Thu Mar 5 20:52:39 2009 Subject: [Histonet] postnatal brain sections using vibratome Message-ID: Dear all I am trying to use vibratome 50 ?m thick sections for immunofluorescence using Postnatal day 0 brains. The problem is that brains are very soft and are usually destroyed upon handling and the agarose is separated form the brain. My used protocol was: perfusion with 4 % PFA for 3 min, followed by several hours to overnight post-fixation. Then embedding brains in 2 % low melting agarose and cutting the block on vibratome using low speed. I am thinking to add an overnight 20 % sucrose incubation step following the post-fixation step. Then embed in agarose and continue the normal protocol. May be sucrose will increase the elasticity of the tissue. So what do you think ? Thanks a lot shymaa From rjbuesa <@t> yahoo.com Fri Mar 6 07:55:00 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 6 07:55:05 2009 Subject: [Histonet] Epithelial cells In-Reply-To: Message-ID: <984047.89521.qm@web65705.mail.ac4.yahoo.com> Try a Papanicolau stain. Ren? J. --- On Thu, 3/5/09, Cheryl Crowder wrote: From: Cheryl Crowder Subject: [Histonet] Epithelial cells To: "Histonet" , "Histonet" , "histonet@lists.utsouthwestern.edu" Date: Thursday, March 5, 2009, 8:59 PM Hi all - We have a graduate student growing equine epithelial cells in culture. Money is really short. She is wondering if there is a regular special stain for these epithelial cells rather than the more expensive IHC. Any suggestions? Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 6 07:55:00 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 6 07:55:07 2009 Subject: [Histonet] Epithelial cells In-Reply-To: Message-ID: <984047.89521.qm@web65705.mail.ac4.yahoo.com> Try a Papanicolau stain. Ren? J. --- On Thu, 3/5/09, Cheryl Crowder wrote: From: Cheryl Crowder Subject: [Histonet] Epithelial cells To: "Histonet" , "Histonet" , "histonet@lists.utsouthwestern.edu" Date: Thursday, March 5, 2009, 8:59 PM Hi all - We have a graduate student growing equine epithelial cells in culture. Money is really short. She is wondering if there is a regular special stain for these epithelial cells rather than the more expensive IHC. Any suggestions? Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Fri Mar 6 07:56:45 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Fri Mar 6 07:56:56 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gcG9zdG5hdGFsIGJyYWluIHNlY3Rpb25zIHVzaW5nIHZpYnJhdG9tZQ==?= Message-ID: <200903062156407916913@foxmail.com> hi, i dont have experience on vibratome section at 50 um (for electrophysiology we cut 300). but I performed a lot on microtome or cryostat - frozen sections at 20-40 um. if you want the brain to be "harder", perfuse longer and have better post-fixation. unless your antigen is more than fragile and even retrieval does not work. embeding could also be achieved by gelatin or egg yolk. Gelatin is softer, while egg yolk is harder and not transparent (you have to mark the direction). The detach problem could be caused by the wet surface of the brain, and the agarose will fall off. 2009-03-06 TF ???? shymaa shawadfy ????? 2009-03-06 10:55:20 ???? histonet@lists.utsouthwestern.edu ??? ??? [Histonet] postnatal brain sections using vibratome Dear all I am trying to use vibratome 50 ? thick sections for immunofluorescence using Postnatal day 0 brains. The problem is that brains are very soft and are usually destroyed upon handling and the agarose is separated form the brain. My used protocol was: perfusion with 4 % PFA for 3 min, followed by several hours to overnight post-fixation. Then embedding brains in 2 % low melting agarose and cutting the block on vibratome using low speed. I am thinking to add an overnight 20 % sucrose incubation step following the post-fixation step. Then embed in agarose and continue the normal protocol. May be sucrose will increase the elasticity of the tissue. So what do you think ? Thanks a lot shymaa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Mar 6 08:31:56 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Mar 6 08:32:01 2009 Subject: [Histonet] GFP Antibody Message-ID: <925359.50865.qm@web1108.biz.mail.sk1.yahoo.com> Hi, I?use Invitrogen's rabbit (A11122) GFP at 1:1000. Paula Pierce, BS, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N Broadway Ave Moore, OK 73160 405-759-3953 www.excaliburpathology.com From Luis.Chiriboga <@t> nyumc.org Fri Mar 6 08:36:02 2009 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Fri Mar 6 08:36:07 2009 Subject: [Histonet] GFP antibody In-Reply-To: <1413635187-1236298267-cardhu_decombobulator_blackberry.rim.net-361284447-@bxe1028.bisx.prod.on.blackberry> References: <1413635187-1236298267-cardhu_decombobulator_blackberry.rim.net-361284447-@bxe1028.bisx.prod.on.blackberry> Message-ID: <87EF241A3ACD47489DC61AEE79BC7D9ECBA7D6@MSGWSDCPMB01.nyumc.org> another option is the antibody from NeoMarkers/Labvision MS-1288. I have used for several years mostly in mouse model but also works in other species and cell lines. have reference if you like + feel free if you have any questions -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anh2006@med.cornell.edu Sent: Thursday, March 05, 2009 7:08 PM To: Michele Wich; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GFP antibody The rabbit pAb antibody from Molecular Probes invitrogen works great in frozen and fixed frozen so it might work in paraffin. Worth a shot.... Andrea ------Original Message------ From: Michele Wich Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mar 5, 2009 6:53 PM Subject: [Histonet] GFP antibody Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From leiker <@t> buffalo.edu Fri Mar 6 08:47:37 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Mar 6 08:47:46 2009 Subject: [Histonet] postnatal brain sections using vibratome In-Reply-To: References: Message-ID: <78BBA76B14408424ABB877EE@bchwxp2702.ad.med.buffalo.edu> Yes, definitely make sure to do the 20% sucrose overnight, even try 30%, or grades going from 10%-20%-30%, switching to the next higher when the brain becomes saturated enough to fall to the bottom of the container. We did this in a previous lab with rat brains and it worked well. Merced --On Friday, March 06, 2009 11:52 AM +0900 shymaa shawadfy wrote: > Dear all > > I am trying to use vibratome 50 ?m thick sections for immunofluorescence > using Postnatal day 0 brains. The problem is that brains are very soft and > are usually destroyed upon handling and the agarose is separated form the > brain. > > My used protocol was: perfusion with 4 % PFA for 3 min, followed by > several hours to overnight post-fixation. Then embedding brains in 2 % > low melting agarose and cutting the block on vibratome using low speed. > > > > I am thinking to add an overnight 20 % sucrose incubation step following > the post-fixation step. Then embed in agarose and continue the normal > protocol. May be sucrose will increase the elasticity of the tissue. > > > > So what do you think ? > > > > > > Thanks a lot > > shymaa > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From froyer <@t> bitstream.net Fri Mar 6 08:51:11 2009 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Mar 6 08:51:21 2009 Subject: [Histonet] histo equipment for sale In-Reply-To: <1191749273.77671236182884723.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> References: <1168878646.77251236182856052.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> <1191749273.77671236182884723.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> Message-ID: <9194BF227215423A89AD86D809437103@Ford> Dear Sightdog(?), You did not give your real name ("Sightdog" doesn't count, unless that IS your real name). You also did not indicate what facility you are with. I'm sure you are on the up and up, but HistoNet Netiquette requires us to identify ourselves and readers would like to know who we are dealing with. What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the equipment located in the lab? Is it still in use? What are you asking, price-wise? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nilfgaard@comcast.net Sent: Wednesday, March 04, 2009 10:08 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] histo equipment for sale Hello histonetters, I have the following, in perfect working condition, items for sale: tissue processor VIP1000 benchtop, just refurbished Shandon 24-3 slide stainer Surgipath PC3001, PC3002 embedding center ducktless hood with new carbon filters hood the a super quite vent system (for outside venting) mini VWR hybridization oven TBS warter bath - like new ONLY, if you are seriously interested, email me at sightdog@comcast.net All pieces are located in Chicagoland. Pics available, can ship UPS ground. thanks Sightdog _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Mar 6 09:09:59 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 6 09:12:00 2009 Subject: [Histonet] histo equipment for sale In-Reply-To: <9194BF227215423A89AD86D809437103@Ford> Message-ID: I know this lab - they are very much legitimate. I would suggest anyone interested in the equipment contact them at the email provided, and they will be happy to talk terms and negotiate price one-on-one. Jackie (aka Cadaverdog) "Ford Royer" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/06/2009 08:51 AM To , cc histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] histo equipment for sale Dear Sightdog(?), You did not give your real name ("Sightdog" doesn't count, unless that IS your real name). You also did not indicate what facility you are with. I'm sure you are on the up and up, but HistoNet Netiquette requires us to identify ourselves and readers would like to know who we are dealing with. What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the equipment located in the lab? Is it still in use? What are you asking, price-wise? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nilfgaard@comcast.net Sent: Wednesday, March 04, 2009 10:08 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] histo equipment for sale Hello histonetters, I have the following, in perfect working condition, items for sale: tissue processor VIP1000 benchtop, just refurbished Shandon 24-3 slide stainer Surgipath PC3001, PC3002 embedding center ducktless hood with new carbon filters hood the a super quite vent system (for outside venting) mini VWR hybridization oven TBS warter bath - like new ONLY, if you are seriously interested, email me at sightdog@comcast.net All pieces are located in Chicagoland. Pics available, can ship UPS ground. thanks Sightdog _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Mar 6 09:17:07 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Mar 6 09:18:39 2009 Subject: [Histonet] histo equipment for sale In-Reply-To: References: Message-ID: <49B13E73.7050309@umdnj.edu> > I know this lab - they are very much legitimate. What lab? Jackie M O'Connor wrote: > I know this lab - they are very much legitimate. I would suggest anyone > interested in the equipment contact them at the email provided, and they > will be happy to talk terms and negotiate price one-on-one. > > Jackie (aka Cadaverdog) > > > > > "Ford Royer" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/06/2009 08:51 AM > > To > , > cc > histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Subject > RE: [Histonet] histo equipment for sale > > > > > > > Dear Sightdog(?), > > You did not give your real name ("Sightdog" doesn't count, unless that IS > your real name). You also did not indicate what facility you are with. > I'm > sure you are on the up and up, but HistoNet Netiquette requires us to > identify ourselves and readers would like to know who we are dealing with. > What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the > equipment located in the lab? Is it still in use? What are you asking, > price-wise? > > Ford M. Royer, MT(ASCP) > Minnesota Medical, Inc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > nilfgaard@comcast.net > Sent: Wednesday, March 04, 2009 10:08 AM > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] histo equipment for sale > > > > > > Hello histonetters, > > I have the following, in perfect working condition, items for sale: > > > > tissue processor VIP1000 benchtop, just refurbished > > Shandon 24-3 slide stainer > > Surgipath PC3001, PC3002 embedding center > > ducktless hood with new carbon filters > > hood the a super quite vent system (for outside venting) > > mini VWR hybridization oven > > TBS warter bath - like new > > > > ONLY, if you are seriously interested, email me at sightdog@comcast.net > > All pieces are located in Chicagoland. Pics available, can ship UPS > ground. > > > > thanks > > Sightdog > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From tifei <@t> foxmail.com Fri Mar 6 09:22:40 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Mar 6 09:22:57 2009 Subject: [Histonet] CD31 on rat brain References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr>, <200903052131230910571@foxmail.com>, <49B00474.2070909@umn.edu> Message-ID: <200903062322352578736@foxmail.com> Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF ???? Colleen Forster ????? 2009-03-06 00:57:29 ???? tifei ??? iskaliora; histonet; ????? ????????? ??? Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > ???? iskaliora > ????? 2009-03-05 18:49:11 > ???? histonet > ??? ????? ????????? > ??? [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Mar 6 09:32:25 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Mar 6 09:33:21 2009 Subject: [Histonet] postnatal brain sections using vibratome In-Reply-To: References: Message-ID: <49B14209.1090400@umdnj.edu> If you put a little glutaraldehyde (0.25%) in the fixaitve you perfuse with but not in the post fix solution the brain will be noticably firmer and your antigen may survive. Good luck! Geoff shymaa shawadfy wrote: > Dear all > > I am trying to use vibratome 50 ?m thick sections for immunofluorescence > using Postnatal day 0 brains. The problem is that brains are very soft and > are usually destroyed upon handling and the agarose is separated form the > brain. > > My used protocol was: perfusion with 4 % PFA for 3 min, followed by several > hours to overnight post-fixation. Then embedding brains in 2 % low melting > agarose and cutting the block on vibratome using low speed. > > > > I am thinking to add an overnight 20 % sucrose incubation step following > the post-fixation step. Then embed in agarose and continue the normal > protocol. May be sucrose will increase the elasticity of the tissue. > > > > So what do you think ? > > > > > > Thanks a lot > > shymaa > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Mar 6 09:35:06 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Mar 6 09:35:11 2009 Subject: [Histonet] In need of AFB controls In-Reply-To: <29381_1236289124_n25Lch3x030223_4EA6CBCAB26218408EFCEC255A8862410796EA92@storm.seattlecca.org> References: <29381_1236289124_n25Lch3x030223_4EA6CBCAB26218408EFCEC255A8862410796EA92@storm.seattlecca.org> Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A0B583E@mailbe01.mc.vanderbilt.edu> If you are an NSH member, you may be able to get your needed controls from the Control Tissue Bank. The "request" form is on the NSH website (www.nsh.org ). The bank is maintained by the Quality Control Committee. You can find contact info for the QC committee chair on the NSH website also. It is a free service for members and they would love to receive control tissue from you as an exchange. If you have any questions that you can't address with the website links, feel free to contact me. Have a great day. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Keller, Jason E [mailto:jkeller@seattlecca.org] Sent: Thursday, March 05, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] In need of AFB controls I am in need of AFB control blocks. They would need to be from human tissue. Willing to consider a trade for other control blocks. Jason E. Keller HTL (ASCP) Seattle Cancer Care Alliance Pathology Department Lab: 206-288-1355 Desk: 206-288-1358 This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From tifei <@t> foxmail.com Fri Mar 6 09:40:31 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Fri Mar 6 09:40:44 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIHBvc3RuYXRhbCBicmFpbiBzZWN0aW9ucyB1c2luZyB2aWJyYXRvbWU=?= References: , <49B14209.1090400@umdnj.edu> Message-ID: <200903062340263789654@foxmail.com> Hi, I dont think glutaraldehyde is good for many antigens...though the brain does become firmer... 2009-03-06 TF ???? Geoff McAuliffe ????? 2009-03-06 23:37:34 ???? shymaa shawadfy ??? histonet ??? Re: [Histonet] postnatal brain sections using vibratome If you put a little glutaraldehyde (0.25%) in the fixaitve you perfuse with but not in the post fix solution the brain will be noticably firmer and your antigen may survive. Good luck! Geoff shymaa shawadfy wrote: > Dear all > > I am trying to use vibratome 50 ? thick sections for immunofluorescence > using Postnatal day 0 brains. The problem is that brains are very soft and > are usually destroyed upon handling and the agarose is separated form the > brain. > > My used protocol was: perfusion with 4 % PFA for 3 min, followed by several > hours to overnight post-fixation. Then embedding brains in 2 % low melting > agarose and cutting the block on vibratome using low speed. > > > > I am thinking to add an overnight 20 % sucrose incubation step following > the post-fixation step. Then embed in agarose and continue the normal > protocol. May be sucrose will increase the elasticity of the tissue. > > > > So what do you think ? > > > > > > Thanks a lot > > shymaa > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Fri Mar 6 09:44:17 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Mar 6 09:44:55 2009 Subject: [Histonet] Re: GFP antibody Message-ID: We have had success staining murine paraffin sections using Rockland goat anti-GFP antibody. You'll need to optimize it to your laboratory conditions trying with either antigen retrieval (citrate buffer pH 6.0) or Proteinase K. Make sure you have tissue with known positive GFP expression and a wild type (negative) animal for controls. As for whether processing destroys the GFP in the sample, it will render the protein non-fluorescent. Fixation alone can do that. But the protein should still be there, even if it is not glowing. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From leiker <@t> buffalo.edu Fri Mar 6 09:50:27 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Mar 6 09:50:32 2009 Subject: [Histonet] postnatal brain sections using vibratome In-Reply-To: <200903062340263789654@foxmail.com> References: , <49B14209.1090400@umdnj.edu> <200903062340263789654@foxmail.com> Message-ID: I also recall having issues in getting antibodies to bind or epitopes to retrieve a year or so ago using 0.5% GA (with the PFA) in perfusing... --On Friday, March 06, 2009 11:40 PM +0800 TF wrote: > Hi, I dont think glutaraldehyde is good for many antigens...though the > brain does become firmer... > > > 2009-03-06 > > > > TF > > > > ???? Geoff McAuliffe > ????? 2009-03-06 23:37:34 > ???? shymaa shawadfy > ??? histonet > ??? Re: [Histonet] postnatal brain sections using vibratome > > If you put a little glutaraldehyde (0.25%) in the fixaitve you perfuse > with but not in the post fix solution the brain will be noticably firmer > and your antigen may survive. > Good luck! > Geoff > shymaa shawadfy wrote: >> Dear all >> >> I am trying to use vibratome 50 ? thick sections for immunofluorescence >> using Postnatal day 0 brains. The problem is that brains are very soft >> and are usually destroyed upon handling and the agarose is separated >> form the brain. >> >> My used protocol was: perfusion with 4 % PFA for 3 min, followed by >> several hours to overnight post-fixation. Then embedding brains in 2 % >> low melting agarose and cutting the block on vibratome using low speed. >> >> >> >> I am thinking to add an overnight 20 % sucrose incubation step following >> the post-fixation step. Then embed in agarose and continue the normal >> protocol. May be sucrose will increase the elasticity of the tissue. >> >> >> >> So what do you think ? >> >> >> >> >> >> Thanks a lot >> >> shymaa >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From ree3 <@t> leicester.ac.uk Fri Mar 6 10:10:16 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Mar 6 10:10:26 2009 Subject: [Histonet] histo equipment for sale In-Reply-To: <9194BF227215423A89AD86D809437103@Ford> References: <1168878646.77251236182856052.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> <1191749273.77671236182884723.JavaMail.root@sz0045a.emeryville.ca.mail.comcast.net> <9194BF227215423A89AD86D809437103@Ford> Message-ID: <7722595275A4DD4FA225B92CDBF174A17455BB4E50@EXC-MBX3.cfs.le.ac.uk> Absolutely Ford!. Best wishes Deputy Dawg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: 06 March 2009 14:51 To: nilfgaard@comcast.net; sightdog@comcast.net Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] histo equipment for sale Dear Sightdog(?), You did not give your real name ("Sightdog" doesn't count, unless that IS your real name). You also did not indicate what facility you are with. I'm sure you are on the up and up, but HistoNet Netiquette requires us to identify ourselves and readers would like to know who we are dealing with. What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the equipment located in the lab? Is it still in use? What are you asking, price-wise? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nilfgaard@comcast.net Sent: Wednesday, March 04, 2009 10:08 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] histo equipment for sale Hello histonetters, I have the following, in perfect working condition, items for sale: tissue processor VIP1000 benchtop, just refurbished Shandon 24-3 slide stainer Surgipath PC3001, PC3002 embedding center ducktless hood with new carbon filters hood the a super quite vent system (for outside venting) mini VWR hybridization oven TBS warter bath - like new ONLY, if you are seriously interested, email me at sightdog@comcast.net All pieces are located in Chicagoland. Pics available, can ship UPS ground. thanks Sightdog _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Fri Mar 6 10:16:29 2009 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Fri Mar 6 10:16:35 2009 Subject: [Histonet] CD31 on rat brain In-Reply-To: <200903062322352578736@foxmail.com> References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr>, <200903052131230910571@foxmail.com>, <49B00474.2070909@umn.edu> <200903062322352578736@foxmail.com> Message-ID: <87EF241A3ACD47489DC61AEE79BC7D9ECBA7DC@MSGWSDCPMB01.nyumc.org> For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). can supply more info if need. The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TF Sent: Friday, March 06, 2009 10:23 AM To: Colleen Forster Cc: histonet; ????? ????????? Subject: [Histonet] CD31 on rat brain Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF ???? Colleen Forster ????? 2009-03-06 00:57:29 ???? tifei ??? iskaliora; histonet; ????? ????????? ??? Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > ???? iskaliora > ????? 2009-03-05 18:49:11 > ???? histonet > ??? ????? ????????? > ??? [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From leiker <@t> buffalo.edu Fri Mar 6 10:20:21 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Mar 6 10:20:27 2009 Subject: [Histonet] histo equipment for sale In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17455BB4E50@EXC-MBX3.cfs.le.ac.uk> References: <1168878646.77251236182856052.JavaMail.root@sz0045a.emeryville.ca .mail.comcast.net> <1191749273.77671236182884723.JavaMail.root@sz0045a.emeryville.ca.mail.comca st.net> <9194BF227215423A89AD86D809437103@Ford> <7722595275A4DD4FA225B92CDBF174A17455BB4E50@EXC-MBX3.cfs.le.ac.uk> Message-ID: <87BB08F1174FD904917D5A06@bchwxp2702.ad.med.buffalo.edu> LOL! It's obviously Friday! - Corn Dog --On Friday, March 06, 2009 4:10 PM +0000 "Edwards, R.E." wrote: > Absolutely Ford!. > Best wishes > Deputy Dawg > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer > Sent: 06 March 2009 14:51 > To: nilfgaard@comcast.net; sightdog@comcast.net > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] histo > equipment for sale > > Dear Sightdog(?), > > You did not give your real name ("Sightdog" doesn't count, unless that IS > your real name). You also did not indicate what facility you are with. > I'm sure you are on the up and up, but HistoNet Netiquette requires us to > identify ourselves and readers would like to know who we are dealing with. > What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the > equipment located in the lab? Is it still in use? What are you asking, > price-wise? > > Ford M. Royer, MT(ASCP) > Minnesota Medical, Inc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > nilfgaard@comcast.net > Sent: Wednesday, March 04, 2009 10:08 AM > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] histo equipment for sale > > > > > > Hello histonetters, > > I have the following, in perfect working condition, items for sale: > > > > tissue processor VIP1000 benchtop, just refurbished > > Shandon 24-3 slide stainer > > Surgipath PC3001, PC3002 embedding center > > ducktless hood with new carbon filters > > hood the a super quite vent system (for outside venting) > > mini VWR hybridization oven > > TBS warter bath - like new > > > > ONLY, if you are seriously interested, email me at sightdog@comcast.net > > All pieces are located in Chicagoland. Pics available, can ship UPS > ground. > > > > thanks > > Sightdog > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From mtc205 <@t> Lehigh.EDU Fri Mar 6 10:23:54 2009 From: mtc205 <@t> Lehigh.EDU (Matthew T Close) Date: Fri Mar 6 10:23:58 2009 Subject: [Histonet] problem with volume 64 issue 11 post Message-ID: <8938460.1236356634227.JavaMail.mtc205@lehigh.edu> There was a major problem with a message I posted yesterday afternoon that appeared today with the subject "re:staining of elastin fibers". Large portions of sentences are completely missing. If need be I can resend. -Matt --------------------------------- Matthew T. Close Lehigh University Department of Biological Sciences From tifei <@t> foxmail.com Fri Mar 6 10:44:32 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Mar 6 10:44:54 2009 Subject: =?utf-8?B?UmU6IFJFOiBbSGlzdG9uZXRdIENEMzEgb24gcmF0IGJyYWlu?= References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr>, <200903052131230910571@foxmail.com>, <49B00474.2070909@umn.edu>, <200903062322352578736@foxmail.com>, <87EF241A3ACD47489DC61AEE79BC7D9ECBA7DC@MSGWSDCPMB01.nyumc.org> Message-ID: <200903070044272168351@foxmail.com> thx. I did notice another anti-rat CD31 from Abcam (they have 41 types of anti-CD31). only mentioned works on Frozen section. How's BD bioscience CD31 work on IHC-Frozen and paraffin sections (animals were fixed with PFA)? 2009-03-07 TF ???? Chiriboga, Luis ????? 2009-03-07 00:16:34 ???? tifei@foxmail.com; Colleen Forster ??? histonet; ????? ????????? ??? RE: [Histonet] CD31 on rat brain For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). can supply more info if need. The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TF Sent: Friday, March 06, 2009 10:23 AM To: Colleen Forster Cc: histonet; ????? ????????? Subject: [Histonet] CD31 on rat brain Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF ???? Colleen Forster ????? 2009-03-06 00:57:29 ???? tifei ??? iskaliora; histonet; ????? ????????? ??? Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > ???? iskaliora > ????? 2009-03-05 18:49:11 > ???? histonet > ??? ????? ????????? > ??? [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From mtc205 <@t> Lehigh.EDU Fri Mar 6 12:27:13 2009 From: mtc205 <@t> Lehigh.EDU (Matthew T Close) Date: Fri Mar 6 12:27:17 2009 Subject: [Histonet] re: staining of elastin fibers (resend) Message-ID: <31106456.1236364033149.JavaMail.mtc205@lehigh.edu> This is a resend of a previous message that seems to have been chopped to pieces in cyberspace. Hope this one comes through better: I prefer to use iron gallein elastin stain for demonstrating elasti fibers. It is simple to make up and gives good contrast compared to some of the other stains. Nuclei stain dark blue, elastin fibers black, everything else is pink or light purple. The protocol can be found in Humanson's Animal tissue techniques and the original article is by Churukian and Schenk (Stain Tech., 1976). I can provide a protocol in .doc format I can send, along with any notes, if you don't have access to either source. Current suppliers of gallein are Fisher Scientific, Spectrum Chemicals, and ArtChemicals.com. Spectrum has it cheapest. Good luck. -Matt --------------------------------- Matthew T. Close Lehigh University Department of Biological Sciences From jm.lapointe <@t> accellab.com Fri Mar 6 12:35:11 2009 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Fri Mar 6 12:35:37 2009 Subject: [Histonet] CD31 on rat brain In-Reply-To: <200903061801.n26I15Yj015386@gateway5.lastspam.com> References: <200903061801.n26I15Yj015386@gateway5.lastspam.com> Message-ID: I would guess that there are problems with the antibodies you are using. SMA is usually a very robust antigen for IHC, if you're not getting any staining you're probably using the wrong antibody. You need an antibody whose specs indicate that it works on mouse, and that it works on fixed tissue. You also don't give details on your staining protocol - it could be a technical issue with that as well. To find proper antibodies, I have found the Biocompare website to be an excellent place to look. Jean-Martin Lapointe AccelLAB Inc ? ? ------------------------------ Message: 11 Date: Sat, 7 Mar 2009 00:44:32 +0800 From: "TF" Subject: Re: RE: [Histonet] CD31 on rat brain [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C??? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr From shimjudy <@t> gmail.com Fri Mar 6 12:46:16 2009 From: shimjudy <@t> gmail.com (Yeonju Shim) Date: Fri Mar 6 12:46:26 2009 Subject: [Histonet] Histology for Zebrafish Message-ID: <7c363b8a0903061046y736a6c54kb35b33f97efb209b@mail.gmail.com> Hi, I need to work on zebrafish for the first time and would like to get some general protocols for fixation and IHC staining. My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and sections didn't come out right (falling off & not preserved well). I am going to increase the time and also try 10% formalin over night. Please let me know if formalin works ok for IHC later. Thank you. Judy From DixonM <@t> vetmed.ufl.edu Fri Mar 6 12:53:05 2009 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Fri Mar 6 12:53:09 2009 Subject: [Histonet] HT exam Message-ID: <530D827EC657DE418C3572ADD63FCDC324967C@EXGVMCNETWORK.vetmed.ufl.edu> Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 From aevans <@t> wellspan.org Fri Mar 6 12:46:27 2009 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Fri Mar 6 12:54:33 2009 Subject: [Histonet] Have a person on 3rd Shift ??? Message-ID: I have posted before, but would like some more feed back on this issue. Thanks to everyone who has already given me feedback on this! I would like to start working a person on third shift to try and relieve some stress for 1st shift. Is there any one who would like to give me their thoughts and opinions on this topic. Did it save money?, Did it relieve stress?? Was it easy to staff? And anything else that it may have helped or hindered. Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From anh2006 <@t> med.cornell.edu Fri Mar 6 12:56:29 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Mar 6 12:56:38 2009 Subject: [Histonet] Histology for Zebrafish In-Reply-To: <7c363b8a0903061046y736a6c54kb35b33f97efb209b@mail.gmail.com> References: <7c363b8a0903061046y736a6c54kb35b33f97efb209b@mail.gmail.com> Message-ID: Have you checked out the ton of protocols (including histo) available at ZFIN online: http://zfin.org/zf_info/zfbook/zfbk.html >Hi, >I need to work on zebrafish for the first time and would like to get some >general protocols for fixation and IHC staining. >My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and >sections didn't come out right (falling off & not preserved well). >I am going to increase the time and also try 10% formalin over night. Please >let me know if formalin works ok for IHC later. >Thank you. >Judy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From katelin <@t> cuttingedgehistology.com Fri Mar 6 13:22:02 2009 From: katelin <@t> cuttingedgehistology.com (Katelin Lester) Date: Fri Mar 6 13:22:10 2009 Subject: [Histonet] HT exam In-Reply-To: <530D827EC657DE418C3572ADD63FCDC324967C@EXGVMCNETWORK.vetmed.ufl.edu> References: <530D827EC657DE418C3572ADD63FCDC324967C@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: <17D87D0C8AF346138F6D18014F63B4BE@Front> Hi MaryAnn, I am also about to take my HTL exam. The website has all the information you are looking for. I've been through these testing facilities when I took my GRE. Make sure you have at least 2 forms of I.D. with you, do not bring a cell phone, etc (all this is on the ASCP website). You will not get any results right away. I don't remember if you can go back and answer missed questions, but I doubt it. The way it works is that there is a pool of about 500+ questions worth at most 900 points. Each question is worth a certain amount of points based on the difficulty of the question. If you get a question correct, the next one will be harder. If you get it wrong, the next will be easier. You will answer 100 questions. Surf through the website and you will find all the answers you seek. (Click on the HT link from this page and a pdf will come up with the testing breakdown) http://www.ascp.org/FunctionalNavigation/certification/GetCertified/Examinat ionContentGuidelines.aspx Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryAnn Dixon Sent: Friday, March 06, 2009 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Mar 6 13:50:30 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Mar 6 13:50:36 2009 Subject: [Histonet] Morgue responsibilities Message-ID: Hi everyone. Happy Friday! We are in the process of reviewing our morgue policies, and the question about ownership has come up. How many of you are responsible for maintaining the morgue? Some hospitals I know have Security in charge, while others say Nursing or the Lab. Who is responsible for repairs, ordering new stretchers, hoists and refrigeration units? If the refrigeration goes down, what is your back-up plan? Do you have an agreement with another hospital, or Medical Examiner? Do you use refrigerated trucks? What is in your Disaster Policy when extra body storage is needed? Thanks in advance for all of your responses. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From igor.deyneko <@t> gmail.com Fri Mar 6 14:04:03 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Fri Mar 6 14:04:08 2009 Subject: [Histonet] Murine Pathologist Needed ASAP in MA area Message-ID: <35e16a770903061204k6c506478s5bec630c9b46cb1@mail.gmail.com> Dear Histonetters! I was wondering if there are any or someone knows a veterinary pathologist who has experience working with mouse tissues and possibly Xenograft models? The pathologist we were outsourcing to has moved to a different state. We need one in Boston or nearby Massachusetts area. Any information would be greatly appreciated. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA 02139 From JMacDonald <@t> mtsac.edu Fri Mar 6 15:54:37 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 6 15:55:23 2009 Subject: [Histonet] HT exam In-Reply-To: <530D827EC657DE418C3572ADD63FCDC324967C@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: MaryAnn, The ASCP uses Computer Adaptive Testing (CAT) for the certification exams. As another person responded your questions will be based on the response to the previous questions. If you get the answer right subsequent questions asked will be of increasing difficulty and worth more points. The maximum score is 999. You need 400 to pass the exam. It is wise to answer each questions to the best of your ability so that you will receive questions with higher point values. You will have an opportunity to review all or some of your responses before you submit the exam. At the conclusion of the exam you will get a message on the screen to indicate that you have passed or failed. You will receive your final score in the mail in about 10 days. Applicants that are not successful will receive a breakdown of the scores by category (fixation, processing, microtomy, staining, and lab operations). Successful applicants just receive the total score. You will have 2.5 hours to answer the 100 questions. 40-50% of the exam is based on staining so that is an area that you will want to concentrate on, but not at the exclusion of the others. The test questions have been updated so don't rely on receiving "old school questions". Also remember that since 2001 cytology preparation is included. You are also responsible for the basic theory of IHC, including antigen retrieval, controls, and antibody preparation. The following link will direct you to an explanation of CAT. http://www.ascp.org/FunctionalNavigation/certification/GetCertified/ComputerAdaptiveTestingCAT.aspx This link will direct you to the examination guidelines for the HT/HTL exams. On the last page is a list of stains to concentrate on. http://www.ascp.org/pdf/ Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "MaryAnn Dixon" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/06/2009 10:53 AM To cc Subject [Histonet] HT exam Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Mar 6 16:03:25 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 6 16:04:00 2009 Subject: [Histonet] HT exam In-Reply-To: Message-ID: http://www.ascp.org/pdf/HTHTLSummaryofStainsforComputerExamination.aspx Sorry. The link was incomplete. Jennifer MacDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 03/06/2009 01:59 PM To "MaryAnn Dixon" cc histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Subject Re: [Histonet] HT exam MaryAnn, The ASCP uses Computer Adaptive Testing (CAT) for the certification exams. As another person responded your questions will be based on the response to the previous questions. If you get the answer right subsequent questions asked will be of increasing difficulty and worth more points. The maximum score is 999. You need 400 to pass the exam. It is wise to answer each questions to the best of your ability so that you will receive questions with higher point values. You will have an opportunity to review all or some of your responses before you submit the exam. At the conclusion of the exam you will get a message on the screen to indicate that you have passed or failed. You will receive your final score in the mail in about 10 days. Applicants that are not successful will receive a breakdown of the scores by category (fixation, processing, microtomy, staining, and lab operations). Successful applicants just receive the total score. You will have 2.5 hours to answer the 100 questions. 40-50% of the exam is based on staining so that is an area that you will want to concentrate on, but not at the exclusion of the others. The test questions have been updated so don't rely on receiving "old school questions". Also remember that since 2001 cytology preparation is included. You are also responsible for the basic theory of IHC, including antigen retrieval, controls, and antibody preparation. The following link will direct you to an explanation of CAT. http://www.ascp.org/FunctionalNavigation/certification/GetCertified/ComputerAdaptiveTestingCAT.aspx This link will direct you to the examination guidelines for the HT/HTL exams. On the last page is a list of stains to concentrate on. http://www.ascp.org/pdf/ Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "MaryAnn Dixon" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/06/2009 10:53 AM To cc Subject [Histonet] HT exam Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Fri Mar 6 16:26:44 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Mar 6 16:27:22 2009 Subject: [Histonet] Masson's trichrome stain Message-ID: I am having a problem with Biebrich Scarlet. I am attempting to do a Masson's stain on some mouse heart tissue. Instead of the strikingly bright red of the muscle tissue I am getting a purple color instead. Everything else looks fine. The hearts are fixed in Penfix and are embedded in paraffin. We make the solution up fresh for each run. I am using Sigma's Ponceau BS (CI 26905). Any ideas? Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. From HornHV <@t> archildrens.org Fri Mar 6 16:35:17 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Mar 6 16:35:23 2009 Subject: [Histonet] Morgue responsibilities In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830A0@EMAIL.archildrens.org> Pathology/Histology is responsible for the autopsy suite, providing the autopsy service, stocking the morgue. Nursing is responsible for the delivery of patients 24/7 to the morgue. Histology is responsible for releasing patients on day shift and arranging the autopsy if one is requested. We have an autopsy assistant who operates our morgue and works in histology when she is not busy providing service to the morgue. Evenings, nights and weekends the nursing service releases the patients. We are responsible for repairs to the autopsy suite. Nursing provides the transport carts. In case of a disaster, another room in the hospital is designated as the temporary morgue. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, March 06, 2009 1:51 PM To: Histonet_Listserv (E-mail) Subject: [Histonet] Morgue responsibilities Hi everyone. Happy Friday! We are in the process of reviewing our morgue policies, and the question about ownership has come up. How many of you are responsible for maintaining the morgue? Some hospitals I know have Security in charge, while others say Nursing or the Lab. Who is responsible for repairs, ordering new stretchers, hoists and refrigeration units? If the refrigeration goes down, what is your back-up plan? Do you have an agreement with another hospital, or Medical Examiner? Do you use refrigerated trucks? What is in your Disaster Policy when extra body storage is needed? Thanks in advance for all of your responses. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kdwyer3322 <@t> aol.com Sat Mar 7 09:23:24 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Mar 7 09:23:39 2009 Subject: [Histonet] 2009 Texas Society for Histotechnology Symposium/Convention Message-ID: <8CB6D4FCAA3FE68-D6C-2D2E@webmail-db17.sysops.aol.com> Hi All, Attached is a preview of the 2009 TSH convention in Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? For more information or a e-mail copy of the program contact: Veronida@baylorhealth.edu or kdywer3322@aol.com Friday, May 29, 2009 WS#1- Skip Brown-Basics Dynamics of Fixation and Processing WS#2 ? Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside Saturday, May 30, 2009 WS#3 - Jan Gardner - Competency Assessment Program WS#4 ? Jennifer Hofecker ? Understanding CJD WS#5 ? Nora Lacey ? 2009 Latest Antibody Information and IHC Applications WS#6 ? David Tate ? Serial Killers WS#7 ? Skip Brown ? The Wax Museum: Understanding Paraffins WS #8 ? Bill DeSalvo/Lamar Jones ? From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab WS#9 ? Donna Willis ? Rapid Tissue Processing, A New Era 2009 WS#10 ? Jim Chalmers ? Pretreatments ? An Important Toll in Immunohistochemistry WS #11 ? Terri Crook, MD ? Cytology for the Histotechnologist WS#12 ? Charlotte Sanders ? Safety Can Be Fun!! Sunday, May 31, 2009 WS #13 ? Jennifer Hofecker ? The Wonderful World of Neuropathology WS #14 ? Bonnie Whitaker/Pam Marcum ? Tw o Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management WS#15 ? Debbie Siena/Brent Hart ? Immunohistochemistry: Validation vs. Optimization WS#16 ? Debra Cohen ? Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) ? ? From kdwyer3322 <@t> aol.com Sat Mar 7 09:23:24 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Mar 7 09:23:43 2009 Subject: [Histonet] 2009 Texas Society for Histotechnology Symposium/Convention Message-ID: <8CB6D4FCAA3FE68-D6C-2D2E@webmail-db17.sysops.aol.com> Hi All, Attached is a preview of the 2009 TSH convention in Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? For more information or a e-mail copy of the program contact: Veronida@baylorhealth.edu or kdywer3322@aol.com Friday, May 29, 2009 WS#1- Skip Brown-Basics Dynamics of Fixation and Processing WS#2 ? Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside Saturday, May 30, 2009 WS#3 - Jan Gardner - Competency Assessment Program WS#4 ? Jennifer Hofecker ? Understanding CJD WS#5 ? Nora Lacey ? 2009 Latest Antibody Information and IHC Applications WS#6 ? David Tate ? Serial Killers WS#7 ? Skip Brown ? The Wax Museum: Understanding Paraffins WS #8 ? Bill DeSalvo/Lamar Jones ? From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab WS#9 ? Donna Willis ? Rapid Tissue Processing, A New Era 2009 WS#10 ? Jim Chalmers ? Pretreatments ? An Important Toll in Immunohistochemistry WS #11 ? Terri Crook, MD ? Cytology for the Histotechnologist WS#12 ? Charlotte Sanders ? Safety Can Be Fun!! Sunday, May 31, 2009 WS #13 ? Jennifer Hofecker ? The Wonderful World of Neuropathology WS #14 ? Bonnie Whitaker/Pam Marcum ? Tw o Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management WS#15 ? Debbie Siena/Brent Hart ? Immunohistochemistry: Validation vs. Optimization WS#16 ? Debra Cohen ? Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) ? ? From jan.innes <@t> runbox.com Sat Mar 7 21:56:40 2009 From: jan.innes <@t> runbox.com (jan.innes@runbox.com) Date: Sat Mar 7 21:56:51 2009 Subject: [Histonet] 2009 Texas Society for Histotechnology In-Reply-To: <8CB6D4FCAA3FE68-D6C-2D2E@webmail-db17.sysops.aol.com> References: <8CB6D4FCAA3FE68-D6C-2D2E@webmail-db17.sysops.aol.com> Message-ID: Hi Can you advise if there is an opportunity to exhibit and / or sponsor a session or both? I am looking at # 5 or 11 or 15. Thanks Jan ----- Start Original Message ----- Sent: Sat, 07 Mar 2009 10:23:24 -0500 From: kdwyer3322@aol.com To: histonet@lists.utsouthwestern.edu, HistoNet@pathology.swmed.edu Subject: [Histonet] 2009 Texas Society for Histotechnology Symposium/Convention > Hi All, > Attached is a preview of the 2009 TSH convention in Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? > For more information or a e-mail copy of the program contact: > Veronida@baylorhealth.edu or kdywer3322@aol.com > > > Friday, May 29, 2009 > > WS#1- Skip Brown-Basics Dynamics of Fixation and Processing > > WS#2 ? Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside > > Saturday, May 30, 2009 > > WS#3 - Jan Gardner - Competency Assessment Program > > WS#4 ? Jennifer Hofecker ? Understanding CJD > > WS#5 ? Nora Lacey ? 2009 Latest Antibody Information and IHC Applications > > WS#6 ? David Tate ? Serial Killers > > WS#7 ? Skip Brown ? The Wax Museum: Understanding Paraffins > > WS #8 ? Bill DeSalvo/Lamar Jones ? From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab > > WS#9 ? Donna Willis ? Rapid Tissue Processing, A New Era 2009 > > WS#10 ? Jim Chalmers ? Pretreatments ? An Important Toll in Immunohistochemistry > > WS #11 ? Terri Crook, MD ? Cytology for the Histotechnologist > > WS#12 ? Charlotte Sanders ? Safety Can Be Fun!! > > Sunday, May 31, 2009 > > WS #13 ? Jennifer Hofecker ? The Wonderful World of Neuropathology > > WS #14 ? Bonnie Whitaker/Pam Marcum ? Tw > o Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management > > WS#15 ? Debbie Siena/Brent Hart ? Immunohistochemistry: Validation vs. Optimization > > WS#16 ? Debra Cohen ? Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) > > ? > > ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----- End Original Message ----- From jan.innes <@t> runbox.com Sat Mar 7 21:56:40 2009 From: jan.innes <@t> runbox.com (jan.innes@runbox.com) Date: Sat Mar 7 21:56:52 2009 Subject: [Histonet] 2009 Texas Society for Histotechnology In-Reply-To: <8CB6D4FCAA3FE68-D6C-2D2E@webmail-db17.sysops.aol.com> References: <8CB6D4FCAA3FE68-D6C-2D2E@webmail-db17.sysops.aol.com> Message-ID: Hi Can you advise if there is an opportunity to exhibit and / or sponsor a session or both? I am looking at # 5 or 11 or 15. Thanks Jan ----- Start Original Message ----- Sent: Sat, 07 Mar 2009 10:23:24 -0500 From: kdwyer3322@aol.com To: histonet@lists.utsouthwestern.edu, HistoNet@pathology.swmed.edu Subject: [Histonet] 2009 Texas Society for Histotechnology Symposium/Convention > Hi All, > Attached is a preview of the 2009 TSH convention in Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? > For more information or a e-mail copy of the program contact: > Veronida@baylorhealth.edu or kdywer3322@aol.com > > > Friday, May 29, 2009 > > WS#1- Skip Brown-Basics Dynamics of Fixation and Processing > > WS#2 ? Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside > > Saturday, May 30, 2009 > > WS#3 - Jan Gardner - Competency Assessment Program > > WS#4 ? Jennifer Hofecker ? Understanding CJD > > WS#5 ? Nora Lacey ? 2009 Latest Antibody Information and IHC Applications > > WS#6 ? David Tate ? Serial Killers > > WS#7 ? Skip Brown ? The Wax Museum: Understanding Paraffins > > WS #8 ? Bill DeSalvo/Lamar Jones ? From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab > > WS#9 ? Donna Willis ? Rapid Tissue Processing, A New Era 2009 > > WS#10 ? Jim Chalmers ? Pretreatments ? An Important Toll in Immunohistochemistry > > WS #11 ? Terri Crook, MD ? Cytology for the Histotechnologist > > WS#12 ? Charlotte Sanders ? Safety Can Be Fun!! > > Sunday, May 31, 2009 > > WS #13 ? Jennifer Hofecker ? The Wonderful World of Neuropathology > > WS #14 ? Bonnie Whitaker/Pam Marcum ? Tw > o Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management > > WS#15 ? Debbie Siena/Brent Hart ? Immunohistochemistry: Validation vs. Optimization > > WS#16 ? Debra Cohen ? Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) > > ? > > ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----- End Original Message ----- From Kimberly.Marshall <@t> ahss.org Mon Mar 9 05:16:43 2009 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Mon Mar 9 05:17:01 2009 Subject: [Histonet] lab week acitivites Message-ID: Last year Histology put together lab week, some of the ideas we came up with Make up a song for the Dept, and had other areas judge (Silly but we all had fun make in up the riffs) sending a example Well you can tell by the way I cut my block I'm a Histotech, no cuttin' through the block Block's cold and slides are warm I've been a cuttin' storm, Since early morn Processed all night, cut today And you may stain another way We can try, to understand The NSH effect on man We also made t-shrits or decorated lab coats with tools in the different depts.. Just some ideas. Hope it helps ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From kmerriam2003 <@t> yahoo.com Mon Mar 9 08:03:49 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Mar 9 08:03:55 2009 Subject: [Histonet] IHC for adenoviral vectors Message-ID: <358143.26179.qm@web50301.mail.re2.yahoo.com> Hi All, I was wondering if anyone has ever done IHC to look for?the?presence of adenoviral vectors in tissue (ie - to track where the viral vector has spread in the body). Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From lpjones <@t> srhs-pa.org Mon Mar 9 09:43:52 2009 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Mar 9 09:43:58 2009 Subject: [Histonet] Cassettes and Processing and Fixation ~ Oh My! Message-ID: <4AE8039AEA096143B965CBC6D092166822D74923@EXCH2007.srhs-pa.org> Hi all. We are having a discussion here about everything in my subject line, but to be more specific: 1. Do you all use different types of cassettes for different sizes of tissues? We have screen cassettes for prostate biopsies, and biopsy cassettes for skins, and regular flow through type for larger-than-they-should-be pieces that have grid marks on them. 2. When you load your cassettes on the processor, do you use the "organized" basket that spaces them out or the "random" basket? If you use the "random" method, how tightly do you feel the cassettes can be packed? Isn't it true that if they are packed too tightly, the fixation of the tissue will be compromised? And, how does everyone use agitation/stirring on the processor? We have always used it, but are wondering how others are doing things. 3. Finally, for a run of combination tissues as described above, what would be your recommended time in formalins? We know all Pathologists are in a hurry for slides, but is a 5 minute station ever acceptable? This is all a result of weeks of discussion about possible changes we could make here, and varying things we have learned over the years... we'd just like to hear what everyone else is doing. For instance: would it be simpler to use biopsy cassettes for everything? Should we consider using the random basket instead of the organized one? How far back could we cut our processing times to expedite things? We'd really appreciate the input of all you experts. Thanks in advance! From Jessica.Vacca <@t> HCAhealthcare.com Mon Mar 9 09:53:16 2009 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Mar 9 09:53:33 2009 Subject: [Histonet] Competency checklist Message-ID: <938D716CD445614ABBB817517557B6F4BC898AFF@NADCWPMSGCMS09.hca.corpad.net> Does anyone have a competency checklist they would like to share, I know all labs are different but if you'd like to share yours, please email it to Jessica.vacca@hcahealthcare.com This would be for Technologist. Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? From rjbuesa <@t> yahoo.com Mon Mar 9 10:02:16 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 9 10:02:21 2009 Subject: [Histonet] Cassettes and Processing and Fixation ~ Oh My! In-Reply-To: <4AE8039AEA096143B965CBC6D092166822D74923@EXCH2007.srhs-pa.org> Message-ID: <435301.6973.qm@web65704.mail.ac4.yahoo.com> Laura: Trying to sum up your concerns, this is what I have always done: 1- just one type of cassette, with different colors?for? rushes, regular, autopsies, bone marrows. If there were small biopsies they were placed between sponges. Bone marrow aspirates wrapped. 2- I always used the organized basket that allowed knowing the number and assured equal spacing between the cassettes 3- the fixation essentially took place before going into the tissue processor but 5 minutes in any event is just a joke. 4- the processing time will depend on the type of tissue and there are limits if you want not to waste the whole run because of inadequate processing Ren? J.? --- On Mon, 3/9/09, Jones, Laura wrote: From: Jones, Laura Subject: [Histonet] Cassettes and Processing and Fixation ~ Oh My! To: "Histonet (E-mail)" Date: Monday, March 9, 2009, 10:43 AM Hi all. We are having a discussion here about everything in my subject line, but to be more specific: 1. Do you all use different types of cassettes for different sizes of tissues? We have screen cassettes for prostate biopsies, and biopsy cassettes for skins, and regular flow through type for larger-than-they-should-be pieces that have grid marks on them. 2. When you load your cassettes on the processor, do you use the "organized" basket that spaces them out or the "random" basket? If you use the "random" method, how tightly do you feel the cassettes can be packed? Isn't it true that if they are packed too tightly, the fixation of the tissue will be compromised? And, how does everyone use agitation/stirring on the processor? We have always used it, but are wondering how others are doing things. 3. Finally, for a run of combination tissues as described above, what would be your recommended time in formalins? We know all Pathologists are in a hurry for slides, but is a 5 minute station ever acceptable? This is all a result of weeks of discussion about possible changes we could make here, and varying things we have learned over the years... we'd just like to hear what everyone else is doing. For instance: would it be simpler to use biopsy cassettes for everything? Should we consider using the random basket instead of the organized one? How far back could we cut our processing times to expedite things? We'd really appreciate the input of all you experts. Thanks in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie <@t> marshfieldclinic.org Mon Mar 9 10:05:02 2009 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Mon Mar 9 10:05:11 2009 Subject: [Histonet] new FS stain problem Message-ID: <200903091505.n29F54sh020787@mailhost2.mfldclin.edu> Good day all, I am doing frozen sectioning at three different facilities in two towns, and this problem is identical at each. The first section I keep, I'm in the habit of placing at the frosted end of the slide. No matter if I take one, two, three or more, that first section is almost non existent hematoxylin, very blurry staining. Thinking I was having contamination, solution level problems, I checked all levels and made sure to have no dripping from the top of the slide, still the same problem. Then, on a whim, I put the first section at the bottom of the slide, and two more working my way toward the frosted end. The first section, placed at the bottom of the slide now, still had the poor staining. Any ideas? I use OCT fix in half and half formalin/95% ETOH DW Harris tap bluing tap eosin 95% X2 100% X2 xylene X2 Let's just say I am a "seasoned" tech, been doing this for almost 30 years, so I'm not a little embarrassed by my inability to figure this one out..... Laurie King Marshfield Clinic Eau Claire/Rice Lake Centers Eau Claire WI From smcbride <@t> cs.cmu.edu Mon Mar 9 10:05:36 2009 From: smcbride <@t> cs.cmu.edu (Sean McBride) Date: Mon Mar 9 10:05:45 2009 Subject: [Histonet] Cassettes and Processing and Fixation ~ Oh My! In-Reply-To: <4AE8039AEA096143B965CBC6D092166822D74923@EXCH2007.srhs-pa.org> Message-ID: Laura, 1. Specimen size dictates to cassette size for our lab. 2. Yes, we use sectioned racks & organize our specimens in sequential order in case the marker is accidentally solvated during the processing. 3. Again, specimen size and tissue type dictate to our fixation schedule, but we never formalin fixate a specimen for only 5 minutes. I prefer to error on the side of caution. ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641 (fax) smcbride@cs.cmu.edu On 3/9/09 10:43 AM, "Jones, Laura" wrote: > Hi all. We are having a discussion here about everything in my subject line, > but to be more specific: > > 1. Do you all use different types of cassettes for different sizes of > tissues? We have screen cassettes for prostate biopsies, and biopsy cassettes > for skins, and regular flow through type for larger-than-they-should-be pieces > that have grid marks on them. > > 2. When you load your cassettes on the processor, do you use the "organized" > basket that spaces them out or the "random" basket? If you use the "random" > method, how tightly do you feel the cassettes can be packed? Isn't it true > that if they are packed too tightly, the fixation of the tissue will be > compromised? And, how does everyone use agitation/stirring on the processor? > We have always used it, but are wondering how others are doing things. > > 3. Finally, for a run of combination tissues as described above, what would > be your recommended time in formalins? We know all Pathologists are in a > hurry for slides, but is a 5 minute station ever acceptable? > > This is all a result of weeks of discussion about possible changes we could > make here, and varying things we have learned over the years... we'd just like > to hear what everyone else is doing. For instance: would it be simpler to use > biopsy cassettes for everything? Should we consider using the random basket > instead of the organized one? How far back could we cut our processing times > to expedite things? We'd really appreciate the input of all you experts. > Thanks in advance! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Mon Mar 9 10:21:02 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Mar 9 10:21:07 2009 Subject: [Histonet] ot? rap for hox genes Message-ID: http://tierneylab.blogs.nytimes.com/2009/03/09/rappin-for-science/?hp i love this song--i work with hox genes in chick emily -- prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves From portera <@t> msu.edu Mon Mar 9 10:21:55 2009 From: portera <@t> msu.edu (Amy Porter) Date: Mon Mar 9 10:22:06 2009 Subject: [Histonet] Competency checklist References: <938D716CD445614ABBB817517557B6F4BC898AFF@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <70D3524D92034881B58D325794731CAB@histolab> The Michigan Society for Histotechnology has a handbook available for purchase through their website www.mihisto.org Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Vacca Jessica" To: Sent: Monday, March 09, 2009 10:53 AM Subject: [Histonet] Competency checklist Does anyone have a competency checklist they would like to share, I know all labs are different but if you'd like to share yours, please email it to Jessica.vacca@hcahealthcare.com This would be for Technologist. Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph.j.kang <@t> pfizer.com Mon Mar 9 10:26:47 2009 From: joseph.j.kang <@t> pfizer.com (Kang, Joseph J) Date: Mon Mar 9 10:26:52 2009 Subject: [Histonet] Zinc Formalin Message-ID: Hey Histonetters, Can anyone tell me about your experiences doing bone IHC using zinc formalin fixative? I also would like to know if anyone have processed rat paws and kee joints using this fixative. If you could provide the optimal fixation time for these tissues would be greatly appreciated. Thanks. Joe From vjp2105 <@t> columbia.edu Mon Mar 9 10:39:46 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Mon Mar 9 10:39:56 2009 Subject: [Histonet] Bone marrow aspirates from mouse Message-ID: Hi everyone, I was wondering if anyone had any experience with taking bone marrow aspirates from mice for analysis, staining with Giemsa and for IHC? My query is, once the aspirate has been taken and then cytospinned down and spread on the slide, what fixative would you use at this point before staining? Maybe formal alcohol? PFA? Or maybe even cytofix spray? also where to store the slides? -80 freezer? Any help on this would be much appreciated, as this procedure has not been carried out here before and I have no experience in this either. Thanks a mill, Vanessa From rjbuesa <@t> yahoo.com Mon Mar 9 10:41:26 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 9 10:41:30 2009 Subject: [Histonet] new FS stain problem In-Reply-To: <200903091505.n29F54sh020787@mailhost2.mfldclin.edu> Message-ID: <35419.98756.qm@web65708.mail.ac4.yahoo.com> I am not completely sure but perhaps that first section taken after cutting down into the block is affected by that triming. Why don't you just discard that first section and start using the following 3 to see if they all stain corretly? Ren? J. --- On Mon, 3/9/09, King, Laurie wrote: From: King, Laurie Subject: [Histonet] new FS stain problem To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 9, 2009, 11:05 AM Good day all, I am doing frozen sectioning at three different facilities in two towns, and this problem is identical at each. The first section I keep, I'm in the habit of placing at the frosted end of the slide. No matter if I take one, two, three or more, that first section is almost non existent hematoxylin, very blurry staining. Thinking I was having contamination, solution level problems, I checked all levels and made sure to have no dripping from the top of the slide, still the same problem. Then, on a whim, I put the first section at the bottom of the slide, and two more working my way toward the frosted end. The first section, placed at the bottom of the slide now, still had the poor staining. Any ideas? I use OCT fix in half and half formalin/95% ETOH DW Harris tap bluing tap eosin 95% X2 100% X2 xylene X2 Let's just say I am a "seasoned" tech, been doing this for almost 30 years, so I'm not a little embarrassed by my inability to figure this one out..... Laurie King Marshfield Clinic Eau Claire/Rice Lake Centers Eau Claire WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 9 10:44:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 9 10:44:06 2009 Subject: [Histonet] Bone marrow aspirates from mouse In-Reply-To: Message-ID: <843059.51343.qm@web65701.mail.ac4.yahoo.com> Use the same thing you would use for a blood smear: methanol 5 minutes and air dry it. Ren? J. --- On Mon, 3/9/09, Vanessa J. Phelan wrote: From: Vanessa J. Phelan Subject: [Histonet] Bone marrow aspirates from mouse To: histonet@lists.utsouthwestern.edu Date: Monday, March 9, 2009, 11:39 AM Hi everyone, I was wondering if anyone had any experience with taking bone marrow aspirates from mice for analysis, staining with Giemsa and for IHC? My query is, once the aspirate has been taken and then cytospinned down and spread on the slide, what fixative would you use at this point before staining? Maybe formal alcohol? PFA? Or maybe even cytofix spray? also where to store the slides? -80 freezer? Any help on this would be much appreciated, as this procedure has not been carried out here before and I have no experience in this either. Thanks a mill, Vanessa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Mon Mar 9 10:50:13 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Mar 9 10:49:19 2009 Subject: [Histonet] mollifax Message-ID: <6.2.3.4.1.20090309084808.0272de70@algranth.inbox.email.arizona.edu> Good Morning, Just wondering - Who sells Mollifax? I'm looking for a good way to soften the exoskeleton on insects of the larger variety. Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Janet.Bonner <@t> FLHOSP.ORG Mon Mar 9 10:47:07 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Mar 9 10:49:36 2009 Subject: [Histonet] new FS stain problem References: <200903091505.n29F54sh020787@mailhost2.mfldclin.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2946@fhosxchmb006.ADVENTISTCORP.NET> When you put the first frozen section on the slide near the label, dip it label side down in the FS fixative so that the fixative just covers the section. Put the consecutive section on the non-label end and then put the whole slide in fixative..Have to fix those sections ASAP!! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of King, Laurie Sent: Mon 3/9/2009 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new FS stain problem Good day all, I am doing frozen sectioning at three different facilities in two towns, and this problem is identical at each. The first section I keep, I'm in the habit of placing at the frosted end of the slide. No matter if I take one, two, three or more, that first section is almost non existent hematoxylin, very blurry staining. Thinking I was having contamination, solution level problems, I checked all levels and made sure to have no dripping from the top of the slide, still the same problem. Then, on a whim, I put the first section at the bottom of the slide, and two more working my way toward the frosted end. The first section, placed at the bottom of the slide now, still had the poor staining. Any ideas? I use OCT fix in half and half formalin/95% ETOH DW Harris tap bluing tap eosin 95% X2 100% X2 xylene X2 Let's just say I am a "seasoned" tech, been doing this for almost 30 years, so I'm not a little embarrassed by my inability to figure this one out..... Laurie King Marshfield Clinic Eau Claire/Rice Lake Centers Eau Claire WI ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From lpaveli1 <@t> hurleymc.com Mon Mar 9 10:57:01 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Mar 9 10:57:23 2009 Subject: [Histonet] new FS stain problem Message-ID: <49B5040D020000EE00027216@smtp-gw.hurleymc.com> I have found that the longer I delay getting the frozen section into fixative, the worse the staining/results. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> Rene J Buesa 03/09/09 11:41 AM >>> I am not completely sure but perhaps that first section taken after cutting down into the block is affected by that triming. Why don't you just discard that first section and start using the following 3 to see if they all stain corretly? Ren? J. --- On Mon, 3/9/09, King, Laurie wrote: From: King, Laurie Subject: [Histonet] new FS stain problem To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 9, 2009, 11:05 AM Good day all, I am doing frozen sectioning at three different facilities in two towns, and this problem is identical at each. The first section I keep, I'm in the habit of placing at the frosted end of the slide. No matter if I take one, two, three or more, that first section is almost non existent hematoxylin, very blurry staining. Thinking I was having contamination, solution level problems, I checked all levels and made sure to have no dripping from the top of the slide, still the same problem. Then, on a whim, I put the first section at the bottom of the slide, and two more working my way toward the frosted end. The first section, placed at the bottom of the slide now, still had the poor staining. Any ideas? I use OCT fix in half and half formalin/95% ETOH DW Harris tap bluing tap eosin 95% X2 100% X2 xylene X2 Let's just say I am a "seasoned" tech, been doing this for almost 30 years, so I'm not a little embarrassed by my inability to figure this one out..... Laurie King Marshfield Clinic Eau Claire/Rice Lake Centers Eau Claire WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Mon Mar 9 11:18:38 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Mar 9 11:17:43 2009 Subject: [Histonet] mollifax In-Reply-To: <6.2.3.4.1.20090309084808.0272de70@algranth.inbox.email.ari zona.edu> References: <6.2.3.4.1.20090309084808.0272de70@algranth.inbox.email.arizona.edu> Message-ID: <6.2.3.4.1.20090309091748.027399e0@algranth.inbox.email.arizona.edu> Excuse me - if I could spell then google might have worked better! Got it! Thanks! Andi At 08:50 AM 3/9/2009, Andrea Grantham wrote: >Good Morning, >Just wondering - Who sells Mollifax? I'm looking for a good way to >soften the exoskeleton on insects of the larger variety. > >Andi >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From KKay <@t> chr.ab.ca Mon Mar 9 12:22:18 2009 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Mon Mar 9 12:22:25 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 15 In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494EF4@exbe.chr.ab.ca> Hello Andrea, We purchase our Mollifex from VWR Scientific Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory,Chinook Regional Hospital 960 - 19 st South Lethbridge, Alberta,CANADA T1J 1W5 (403) 388 - 6061 (Phone) (403) 388 - 6067 (Fax) ------------------------------ Message: 14 Date: Mon, 09 Mar 2009 08:50:13 -0700 From: Andrea Grantham Subject: [Histonet] mollifax To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.1.20090309084808.0272de70@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Good Morning, Just wondering - Who sells Mollifax? I'm looking for a good way to soften the exoskeleton on insects of the larger variety. Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From eca9 <@t> georgetown.edu Mon Mar 9 14:25:32 2009 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Mon Mar 9 14:25:41 2009 Subject: [Histonet] avidin/biotin blocking??? Message-ID: <49B56D2C.9030103@georgetown.edu> Good afternoon out there in histoland, I am trying to find a cheaper alternative to the avidin/biotin blocking system we are using now so that we can use it for larger bulk staining cases. Right now we only use it for small hand staining cases. I read somewhere that there are those who make these reagents themselves. Would anyone be willing to share their recipes and how do they compare to company bought ones? Thanks for your help, Eva Permaul Georgetown University From jcrawfor <@t> aerotek.com Mon Mar 9 14:45:53 2009 From: jcrawfor <@t> aerotek.com (Crawford, Jennifer) Date: Mon Mar 9 14:46:31 2009 Subject: [Histonet] Job Opportunity: Histotechnologist Message-ID: <571A823E0300F549BE86279A872395770263ECAE@ag00-exmbx04.allegisgroup.com> Title: Histotechnologist Location: South suburbs of Chicago Pay: $25-27/hr based on experience Employment Type: 6 month contract to hire Shift: 1st shift (4:30 AM - 2:30 PM or 5:30 AM - 3:30 PM, Monday through Thursday with rotating Saturday schedule) Part time positions are available as well!!! Requirements: Under the leadership of the Director and section supervisor, the Histology Technologist receives and properly processes specimens for the Pathologists in a timely manner, reviews test results, and participates in Quality Assurance. The histology technician will also maintain slide files and records, assist the Pathologist and function in a limited capacity as a cytotechnologist as needed. Specific required skills: Immunostaining (Ventana Stainer), Cytology, ASCP certification is a must. ***Please contact Jen Crawford at jcrawfor@aerotek.com or by phone at (847) 221-1358. *** Jen Crawford Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! Aerotek has become the #1 staffing provider within the sciences! http://www.aerotek.com/About-Us/Press-Release-10147.news ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From LBlack <@t> carilion.com Mon Mar 9 14:51:15 2009 From: LBlack <@t> carilion.com (Lisa S. Black) Date: Mon Mar 9 14:47:45 2009 Subject: [Histonet] Tissue specimen disposal Message-ID: <49B53AF3020000E8000209AA@chs-gw-gwia2.carilion.com> 1. How do you dispose of formalin fixed tissue specimens? 2. If you use a vendor, do you pour off the formalin first and have two separate waste companies remove the fluid and tissue? 3. If you use one vendor for both, please provide vendor name. Thanks, Lisa From MSHERWOOD <@t> PARTNERS.ORG Mon Mar 9 14:58:32 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Mar 9 14:58:43 2009 Subject: [Histonet] Tissue specimen disposal In-Reply-To: <49B53AF3020000E8000209AA@chs-gw-gwia2.carilion.com> References: <49B53AF3020000E8000209AA@chs-gw-gwia2.carilion.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2388C@PHSXMB30.partners.org> Lisa, I just had a similar situation, and our waste management company just had me put the tightly sealed containers in plastic bags and label with hazardous waste label. I could put multiple samples in a plastic bag. I thought I would have to separate the tissue from the fixative, but that was not the case. I am in Boston, and we deal with Triumverate Environmental. Hope this helps! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa S. Black Sent: Monday, March 09, 2009 3:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue specimen disposal 1. How do you dispose of formalin fixed tissue specimens? 2. If you use a vendor, do you pour off the formalin first and have two separate waste companies remove the fluid and tissue? 3. If you use one vendor for both, please provide vendor name. Thanks, Lisa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From laurie.colbert <@t> huntingtonhospital.com Mon Mar 9 15:08:55 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Mar 9 15:09:04 2009 Subject: [Histonet] Tissue specimen disposal Message-ID: <57BE698966D5C54EAE8612E8941D768305294C3F@EXCHANGE3.huntingtonhospital.com> We have an outside vendor, Stericycle, separate the formalin and the tissue, and they take both for disposal. I am in the Los Angeles area. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa S. Black Sent: Monday, March 09, 2009 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue specimen disposal 1. How do you dispose of formalin fixed tissue specimens? 2. If you use a vendor, do you pour off the formalin first and have two separate waste companies remove the fluid and tissue? 3. If you use one vendor for both, please provide vendor name. Thanks, Lisa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mark <@t> vyleater.com Mon Mar 9 16:12:55 2009 From: mark <@t> vyleater.com (Mark J. Griffith) Date: Mon Mar 9 16:13:06 2009 Subject: Fwd: [Histonet] Tissue specimen disposal Message-ID: <20090309211259.DRJK20891.outaamta02.mail.tds.net@marklt.ramflat.com> Lisa, We have a few customers who use the Vyleater to shred the specimen vial and reclaim the formalin for bulk disposal. By gathering the formalin in bulk form - the waste disposal savings can be considerable. Most are disposing of the shredded plastic as chemical hazardous or bio hazardous waste depending upon how their safety folks evaluate the wastestream. But in either case, removing the liquid makes a big difference. Contact me directly if you would like to get more details. Mark Griffith S & G Enterprises, Inc. N115 W19000 Edison Drive Germantown, WI 53022 USA Tel: 262/251-8300 US/Canada toll-free: 800/233-3721 Fax: 262/251-1616 ====Original Message==== >Date: Mon, 09 Mar 2009 15:51:15 -0400 >From: "Lisa S. Black" >To: >Subject: [Histonet] Tissue specimen disposal >Sender: histonet-bounces@lists.utsouthwestern.edu > >1. How do you dispose of formalin fixed tissue specimens? >2. If you use a vendor, do you pour off the formalin first and have >two separate waste companies remove the fluid and tissue? >3. If you use one vendor for both, please provide vendor name. > >Thanks, >Lisa > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atellez <@t> crf.org Tue Mar 10 08:02:25 2009 From: atellez <@t> crf.org (Armando Tellez) Date: Tue Mar 10 08:03:02 2009 Subject: [Histonet] Tissue RE-processing In-Reply-To: References: Message-ID: Hi to everybody, I have a problem with a recent processing tissue. Normally I process tissue that is very small such as 3mm thick of vessel rings or small cubes of myocardium. Couple of days ago we process a batch of myocardial sections that are really thick. I thought might be thick enough my protocol to perfuse completely. I was wrong it was too thick after the overnight protocol the sections were moist and soft. Of course I didn't even try to embedded them. Question, what can I do now? Can I reprocess them? Once they certain amount of wax in them can I put them again through the alcohols and xylenes and again through paraffin? Or is better just to dry them immersing them in alcohol? I just change the solutions of the processor so before actually going ahead and process them again I preferred to ask since I wouldn't like to get all the solutions dirty with wax with the need to rechange them once again. I appreciate your answers and help. Armando Tellez atellez@crf.org Pathology Department Cardiovascular Research Foundation Skirball Center for Cardiovascular Research 8 Corporate Drive Orangeburg, New York,10962 From alyssa <@t> alliedsearchpartners.com Tue Mar 10 09:51:51 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Mar 10 09:51:57 2009 Subject: [Histonet] Managment Opportunity In Histology-in Mass Message-ID: Good Morning, My name is Alyssa Peterson, and I am the Director of Lab/Pathology recruitment, and I am contacting you about a permanent, full time, Monday-Friday Day Shift Histology Management position located in about 20 miles North West of Boston (You would not have to travel into Boston)! If you are interested please respond with a current copy of your resume attached as a Microsoft word document, and a contact number with the best time to reach you. You can call me anytime, at 770.621.2639 ext. 4 or send me an email at alyssa@alliedsearchpartners.com for further information. If you are not interested we offer a $1000 referral bonus so feel free to forward this email to whomever you feel fit. ***Please only serious inquiries only*** Please review the job description: Prior experience in histology laboratory with certification. BS Degree in Life science from accredited college classes with histology experience. Ability to run the histology operations, manage the staff and interact with upper management. Day Shift, Mon-Friday 8am-5pm. Benefits: They offer an attractive benefits package including medical/dental insurance, PTO, 401K, and much more!!!!!! Salary is highly competitive!!! *If you are in need of a laboratory professional, please remember to ask me about how our new clients are eligible for our 5K flat rate on any laboratory position at your facility!* -- -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From NLinke <@t> mednet.ucla.edu Tue Mar 10 10:05:41 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Tue Mar 10 10:05:45 2009 Subject: [Histonet] 2 histology positions at UCLA Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE442F36116@EMGMB1.ad.medctr.ucla.edu> Hi all, We still have 2 positions available here at UCLA. The first is a histotech I position, a 50/50 clinical/research position working with us in the clinical lab as well as in the lab of one of our urologic pathology faculty (a great guy I might add). While not a requirement, if you have experience in whole mount prostate and/or manual IHC, FISH etc that would be wonderful! The salary range for the histotech position is $31.53-$37.68 per hour DOE. The second position is for a laboratory assistant/tech. This position involves staining, coverslipping, sending out slides, maintaining equipment, running the Ventana special stainers, answering phones etc. The salary range for the laboratory assistant/tech position is $19.56-$25.80 per hour DOE. Please feel free to contact me or check out our website https://jobs2.mednet.ucla.edu/css_external/CSSPage_Welcome.ASP . Both positions have been listed, and FYI the hours listed on the site for the HTLI position are incorrect. Hours have not been determined yet, but will more than likely will be first or second (day) shift. The Job number for the HTLI position is H49313 and the laboratory assistant/tech is H49330. Thank you! Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA 10833 Le Conte Ave A3-172 Los Angeles, CA 90095 Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From Barbara.Stancel <@t> fsis.usda.gov Tue Mar 10 10:09:31 2009 From: Barbara.Stancel <@t> fsis.usda.gov (Stancel, Barbara) Date: Tue Mar 10 10:09:43 2009 Subject: [Histonet] RE: Cassettes and Processing and Fixation ~ Oh My! Message-ID: Laura, 1. In the past we used different colored cassettes for rush, non rush and special projects. Then we noticed that we still treated every case like it was retained/rush!! So we dropped back to one color for all our regular cases; microscreen cassettes for anything that might slip through and for our comminuted meat products. 2. Day (3 hours) run blocks are "dumped" in (i.e. not organized). We don't usually have any problems except with the tissues cut by one pathologist - who cuts too fast and sometimes too thick. But since I am here alone in the wee hours of the mornings to embed, I prefer my night (16 hrs run) blocks organized in neat 20 block rows and a lid placed on the basket. Our baskets do not have organizing spacers. We pack'em tight for long overnight runs! We have never had infiltration problems with them. We use pressure/vacuum (both VIP E300s) on all stations, all runs. 3. Our tissues are shipped overnight. So 99% of them are received nicely fixed. Exception: winter time-and tissues being shipped from the Northern states and Canada. If they are received in formalin and are under-fixed, after grossing, we transfer them to warm formalin until they go on the processor, then we give them 10 minutes on the processor with heat/vacuum/ pressure. All tissues arriving after 10 AM are held for overnight. Approximately 50% of cases are completed and diagnosis reported in 8-9 hours after arrival. The next 45% are completed in 24 hrs. The last 5% are completed within 72 hours from arrival. We started cay runs in 1978. We used an Ultra AutoTechnicon (with an agitating basket) followed several years later by the Fisher Histomatic (with an adjustable-speed stir bar in the bottom of the chamber):both processors had a direct method of stirring. We were able to process tissues in 2 hrs. When we began our search for a new processor in the 1990's, there were no processors with a stirring bar in the bottom of the processing chamber-our processing times increased to compensate for reduced agitation. Good luck in your quest to shorten processing and turn-around times. We have all struggled with the same questions. And through trial-and-error (or trial-by-fire!) ultimately found the times right for each lab's unique circumstances and problems. Histologically yours, Barbara Barbara Stancel, HTL(ASCP) Lead Technologist USDA, FSIS, EL, Pathology RRC, 950 College Station Road Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stancel@fsis.usda.gov No trees were hurt in the sending of this e-mail. However many electrons were severly inconvenienced! From Jackie.O'Connor <@t> abbott.com Tue Mar 10 10:32:14 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Mar 10 10:32:37 2009 Subject: [Histonet] Looking for Darcy Peat In-Reply-To: <49B56D2C.9030103@georgetown.edu> Message-ID: I worked with Darcy in Hawaii years ago, and I think someone said she may have moved to Washington State. Darcy, are you out there? Does anyone know where she is? Jackie O' From arvidsonkristen <@t> yahoo.com Tue Mar 10 10:53:54 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Mar 10 10:54:03 2009 Subject: [Histonet] (no subject) Message-ID: <186210.27094.qm@web65701.mail.ac4.yahoo.com> Does anyone have an opinion on Distilled vs DI water?? Is there a difference in quality? From SharonC <@t> celligent.net Tue Mar 10 10:55:40 2009 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Tue Mar 10 10:55:45 2009 Subject: [Histonet] metal molds vs. disposable molds Message-ID: Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net From gu.lang <@t> gmx.at Tue Mar 10 10:56:43 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 10 10:56:57 2009 Subject: AW: [Histonet] Tissue RE-processing In-Reply-To: References: Message-ID: <9365FC27DC2C4E16BC31293D3146A8E4@dielangs.at> You can go back into warm xylen for one hour, that removes the paraffin. Then let the tissue in 2 changes of ethanol abs. , one hour each. That should be long enough for dehydration and hardening the tissue. Then go into xylen again for one hour, and then let it sit in paraffin for 2-3 hours. The duration in paraffin is rather long, because there's no vacuum, that helps infiltration. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Armando Tellez Gesendet: Dienstag, 10. M?rz 2009 14:02 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Tissue RE-processing Hi to everybody, I have a problem with a recent processing tissue. Normally I process tissue that is very small such as 3mm thick of vessel rings or small cubes of myocardium. Couple of days ago we process a batch of myocardial sections that are really thick. I thought might be thick enough my protocol to perfuse completely. I was wrong it was too thick after the overnight protocol the sections were moist and soft. Of course I didn't even try to embedded them. Question, what can I do now? Can I reprocess them? Once they certain amount of wax in them can I put them again through the alcohols and xylenes and again through paraffin? Or is better just to dry them immersing them in alcohol? I just change the solutions of the processor so before actually going ahead and process them again I preferred to ask since I wouldn't like to get all the solutions dirty with wax with the need to rechange them once again. I appreciate your answers and help. Armando Tellez atellez@crf.org Pathology Department Cardiovascular Research Foundation Skirball Center for Cardiovascular Research 8 Corporate Drive Orangeburg, New York,10962 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 10 10:59:48 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 10 10:59:56 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: Message-ID: <174517.92419.qm@web65714.mail.ac4.yahoo.com> Metals molds are easier to work with and almost indestructible, but if you have money to throw to the air?and patient and time?in large supply, use plastic. Ren? J. --- On Tue, 3/10/09, Sharon Campbell wrote: From: Sharon Campbell Subject: [Histonet] metal molds vs. disposable molds To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 11:55 AM Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Mar 10 11:03:18 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Mar 10 11:03:36 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: <174517.92419.qm@web65714.mail.ac4.yahoo.com> References: <174517.92419.qm@web65714.mail.ac4.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF033E@LTA3VS011.ees.hhs.gov> I agree. I also find you get a more level plane which makes trimming easier. We use both in our group and the individuals that use the disposable tend to use them multiple times before tossing them. I have not had to clean the metal ones I use in years but have heard you can toss them in the clean cycle of your tissue processor. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 10, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu; Sharon Campbell Subject: Re: [Histonet] metal molds vs. disposable molds Metals molds are easier to work with and almost indestructible, but if you have money to throw to the air?and patient and time?in large supply, use plastic. Ren? J. --- On Tue, 3/10/09, Sharon Campbell wrote: From: Sharon Campbell Subject: [Histonet] metal molds vs. disposable molds To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 11:55 AM Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Mar 10 11:03:40 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Mar 10 11:03:53 2009 Subject: [Histonet] metal molds vs. disposable molds References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F294D@fhosxchmb006.ADVENTISTCORP.NET> Metal molds here....we tried the plastic molds but the base would rise up creating an indentation in the block, we needed a flat surface. We wash our molds in an old processor, using the old baskets and running a xylene to alcohol to 95% EtOH run. Then we spread them on a towel to dry and spray them with a mold release. We still use the small plastic molds for the biopsies, however. The metal molds are great for cooling quickly. In another lab I worked in, we used all plastic molds - and to not have to wash the molds, just replace as needed, was heaven! I would recommend trying each and see what works for your lab. Janet L. Bonner, HTL (ASCP) Pathology Laboratory ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon Campbell Sent: Tue 3/10/2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] metal molds vs. disposable molds Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From tkngflght <@t> yahoo.com Tue Mar 10 11:16:42 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Mar 10 11:16:46 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: Message-ID: <537309.27352.qm@web50909.mail.re2.yahoo.com> Hi Sharon- ? I've been on your side of this quandry before.? A couple of considerations: what kinds of tissue are you embedding? How many blocks/shift? What is the most important aspect(s) of the following for your situation? ? The pros and cons: Metal - pro cool fast easy to remove once cold the base doesn't concave on the bigger sizes quicker response to hot/cold (easier to warm/cool for tissue handling during embedding & reembedding) ? Metal - con have to be cleaned (can set on side and pass thru processor though this will spark a raging debate on using a processor for cleaning--REALLY hot soapy water works well, too) expensive up front cost ? Plastic - pro square bottoms--full face sections with less trimming (great for prost bx) no need to clean can use more than one time to reduce cost but will crack eventually CLEAR--can see how things are oriented on the fly ? Plastic - con harder to pop (have to be all the way hardened or the bottom can stick in the well) longer to cool ongoing expense larger sizes can 'convex' depending on embedding medium shrinkage ? I'm sure the Histonetters?can add many points to this list.? It comes down to what do you need most on a weighted scale of pro and con?? In the end, I usually choose to use both--keeps costs down and gives us options and once in a while when you get back-ordered on the plastic you're not forced to fold paper boats like we did 30 years ago!! ? Hope this helps! ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time 800.756.3309 --- On Tue, 3/10/09, Sharon Campbell wrote: From: Sharon Campbell Subject: [Histonet] metal molds vs. disposable molds To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 8:55 AM Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Mar 10 11:22:28 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 10 11:22:33 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: References: Message-ID: I like my metal molds. They are neat, you get even heat transfer, and are easy to clean when they need it. I clean them by tossing them in some boiling water with an excess of cleaning powder (Alconox or Sparkleen) or any detergent you have on hand (make sure it doesn't get too bubbly and froth over though). Then I rinse them under hot top water, spread on paper towels to dry, spray with mold release, and stack in the oven. Merced --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell wrote: > Hello everyone, > > We have a debate going on about purchasing metal molds vs. disposable > plastic molds. Which is better? Is the cost better in the long run to > buy metal? Also, how does everyone clean their metal molds? > > Thanks > > > > Sharon Campbell, HTL(ASCP)CM, BSBM > > Histology Supervisor > > Celligent Diagnostics, LLC > > Formerly Pathology Associates Services > > 101 East W.T. Harris Blvd, Suite 1212 > > Charlotte, NC 28262 > > 800-524-6779 ext. 104 > > 704-970-3304 Direct Line > > sharonc@celligent.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From cmiller <@t> physlab.com Tue Mar 10 11:45:02 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Mar 10 11:45:08 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: References: Message-ID: <000001c9a19f$8f3d21b0$4402a8c0@plab.local> My issue with the plastic disposable molds is that when you need to re-embed and melt the block down, the mold will curl and lose its shape just enough to impede cutting the second time around. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Tuesday, March 10, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] metal molds vs. disposable molds Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From SohrabB1 <@t> ah.org Tue Mar 10 11:49:32 2009 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue Mar 10 11:50:20 2009 Subject: [Histonet] CAP Message-ID: <49B637AC.4347.0054.0@ah.org> Does any one have a procedure regarding : " Handling of work load during instrument failure" .? This would include, VIP,embedding ,etc... Behnaz Sohrab From JWeems <@t> sjha.org Tue Mar 10 11:59:06 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 10 11:59:22 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53AC231@ITSSSXM01V6.one.ads.che.org> And if you let the water cool, the paraffin will harden and you discard it without pouring down the drain... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Tuesday, March 10, 2009 12:22 PM To: Sharon Campbell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] metal molds vs. disposable molds I like my metal molds. They are neat, you get even heat transfer, and are easy to clean when they need it. I clean them by tossing them in some boiling water with an excess of cleaning powder (Alconox or Sparkleen) or any detergent you have on hand (make sure it doesn't get too bubbly and froth over though). Then I rinse them under hot top water, spread on paper towels to dry, spray with mold release, and stack in the oven. Merced --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell wrote: > Hello everyone, > > We have a debate going on about purchasing metal molds vs. disposable > plastic molds. Which is better? Is the cost better in the long run to > buy metal? Also, how does everyone clean their metal molds? > > Thanks > > > > Sharon Campbell, HTL(ASCP)CM, BSBM > > Histology Supervisor > > Celligent Diagnostics, LLC > > Formerly Pathology Associates Services > > 101 East W.T. Harris Blvd, Suite 1212 > > Charlotte, NC 28262 > > 800-524-6779 ext. 104 > > 704-970-3304 Direct Line > > sharonc@celligent.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From gu.lang <@t> gmx.at Tue Mar 10 12:22:28 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 10 12:22:36 2009 Subject: AW: [Histonet] metal molds vs. disposable molds In-Reply-To: References: Message-ID: We clean our metal molds in an ultra-sound cleaner. Fill it with warm water and a bit of dishcleaner, let it for 10 min. The paraffin easy goes off and swims on the surface. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sharon Campbell Gesendet: Dienstag, 10. M?rz 2009 16:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] metal molds vs. disposable molds Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Mar 10 12:36:54 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 10 12:36:59 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA53AC231@ITSSSXM01V6.one.ads.che.org> References: <5D64396A0D4A5346BEBC759022AAEAA53AC231@ITSSSXM01V6.one.ads.che.org> Message-ID: <58764FED08812DF8952DFD90@bchwxp2702.ad.med.buffalo.edu> ...which might be the better thing to do then...since it'll all be floating on the surface anyway and we don't want to risk clogging drain pipes with the wax once it cools... --On Tuesday, March 10, 2009 12:59 PM -0400 "Weems, Joyce" wrote: > And if you let the water cool, the paraffin will harden and you discard > it without pouring down the drain... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced > Leiker > Sent: Tuesday, March 10, 2009 12:22 PM > To: Sharon Campbell; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] metal molds vs. disposable molds > > I like my metal molds. They are neat, you get even heat transfer, and > are easy to clean when they need it. I clean them by tossing them in > some boiling water with an excess of cleaning powder (Alconox or > Sparkleen) or any detergent you have on hand (make sure it doesn't get > too bubbly and froth over though). Then I rinse them under hot top > water, spread on paper towels to dry, spray with mold release, and stack > in the oven. > > Merced > > --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell > wrote: > >> Hello everyone, >> >> We have a debate going on about purchasing metal molds vs. disposable >> plastic molds. Which is better? Is the cost better in the long run to >> buy metal? Also, how does everyone clean their metal molds? >> >> Thanks >> >> >> >> Sharon Campbell, HTL(ASCP)CM, BSBM >> >> Histology Supervisor >> >> Celligent Diagnostics, LLC >> >> Formerly Pathology Associates Services >> >> 101 East W.T. Harris Blvd, Suite 1212 >> >> Charlotte, NC 28262 >> >> 800-524-6779 ext. 104 >> >> 704-970-3304 Direct Line >> >> sharonc@celligent.net >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences State University of New York > at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From mwich <@t> 7thwavelabs.com Tue Mar 10 13:34:04 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Mar 10 13:34:08 2009 Subject: [Histonet] histological stain for pancreatic beta cells Message-ID: <62A8156F8071C8439080D626DF8C33A65D8B94@wave-mail.7thwave.local> Can I please get some suggestions for histological stains that demonstrate pancreatic beta cells? I need something that would be highly specific without using an IHC method. Any advice is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From zodiac29 <@t> comcast.net Tue Mar 10 13:42:32 2009 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Tue Mar 10 13:42:35 2009 Subject: [Histonet] Ci-Trol Message-ID: <1531333586.5049751236710552549.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Hello Everyone, Is anyone familiar with Ci-trol (citrated plasma) for making cell blocks? Someone had recommended a procedure using this, and I wanted to get some feedback on it. Thanks in advance! Jenny From barrickstacey <@t> yahoo.com Tue Mar 10 13:51:02 2009 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Tue Mar 10 13:51:07 2009 Subject: [Histonet] histological stain for pancreatic beta cells In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B94@wave-mail.7thwave.local> Message-ID: <66066.42121.qm@web54302.mail.re2.yahoo.com> We have done an anti-insulin antibody (Abcam) with a hemotoxylin counterstain. Use diaminobenzidine-H2O2? (BioGenex) to?visualize the antibody.? --- On Tue, 3/10/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] histological stain for pancreatic beta cells To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 2:34 PM Can I please get some suggestions for histological stains that demonstrate pancreatic beta cells? I need something that would be highly specific without using an IHC method. Any advice is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Tue Mar 10 14:39:17 2009 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Tue Mar 10 14:39:35 2009 Subject: [Histonet] 2009 Tri-State Symposium Message-ID: <2108AECB05DBFF48A9C436A7921557408478B5@UWHC-MAIL03.uwhis.hosp.wisc.edu> You are invited to join the circus and clown around with the histology societies of Wisconsin, Minnesota and Iowa as they host "Big Top Histology" the 2009 Tri-State Symposium, April 29-May 1, at The Madison Concourse Hotel, Madison, Wisconsin. The inclusive registration fee of $100 (+ state membership) covers workshops, seminars, lunches and our infamous costume banquet. The 2009 banquet theme will be "Histology Under the Big Top; It's A 3 Ring Laboratory Circus Out There". CEU Approved Workshops & Seminars Offered: Wednesday April 29th: Improving Lab Processes With the Use of Automatic Identification Thursday April 30th: The Basic Chemistry of Histological Staining, To Err Is Human; Patient Safety is Divine, An Introduction to Molecular Pathology, The Wild World of Neuropathology, Pretreatments-An Important Tool In IHC. Friday May 1st: Current Orthopedic Treatments for Pets, Mohs Surgery for Ocular Sebaceous Carcinoma Using ORO, Digital Imaging In Contract Research, Interpretation of Needle Core Biopsies in Evaluation of Non-Neoplastic Liver Diseases, Processing Schedules Revisited, Understanding CJD, Stars of the Silver Screen: Troubleshooting Basics for Silver Stains, IHC Basics & Beyond. For further information contact any of the states representatives: Wisconsin: Maureen Decorah (decorah@wisc.edu) or Jean Mitchell (jmitchell@uwhealth.org) Minnesota: Ann Maruska (amaruska@mngastro.com) or Lois Rowe (rowe.lois@mayo.edu) Iowa: Judi Stasko (judith.stasko@ars.usda.gov) or Dave Cavanaugh (dcavan55@yahoo.com) From rjbuesa <@t> yahoo.com Tue Mar 10 15:04:33 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 10 15:04:36 2009 Subject: [Histonet] CAP In-Reply-To: <49B637AC.4347.0054.0@ah.org> Message-ID: <298940.32321.qm@web65714.mail.ac4.yahoo.com> This aspect has to be part of your contingency plan and each lab, with its own idiosyncrasies, has to prepare one, like "what to do during/after a hurricane". Ren? J. --- On Tue, 3/10/09, Behnaz Sohrab wrote: From: Behnaz Sohrab Subject: [Histonet] CAP To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 12:49 PM Does any one have a procedure regarding : " Handling of work load during instrument failure" .? This would include, VIP,embedding ,etc... Behnaz Sohrab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 10 15:06:52 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 10 15:06:55 2009 Subject: [Histonet] histological stain for pancreatic beta cells In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B94@wave-mail.7thwave.local> Message-ID: <233008.62095.qm@web65708.mail.ac4.yahoo.com> Mallory Azan. Ren? J. --- On Tue, 3/10/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] histological stain for pancreatic beta cells To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 2:34 PM Can I please get some suggestions for histological stains that demonstrate pancreatic beta cells? I need something that would be highly specific without using an IHC method. Any advice is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Mar 10 15:11:12 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 10 15:11:20 2009 Subject: [Histonet] histological stain for pancreatic beta cells In-Reply-To: <233008.62095.qm@web65708.mail.ac4.yahoo.com> References: <233008.62095.qm@web65708.mail.ac4.yahoo.com> Message-ID: <200B375EFF25BC3ED2E4E881@bchwxp2702.ad.med.buffalo.edu> I just searched for this and found in the Histonet archives - currently Google's #1 hit: http://www.histosearch.com/histonet/Sep07/RE.HistonetAzan-MalloryB.html --On Tuesday, March 10, 2009 1:06 PM -0700 Rene J Buesa wrote: > Mallory Azan. > Ren? J. > > --- On Tue, 3/10/09, Michele Wich wrote: > > From: Michele Wich > Subject: [Histonet] histological stain for pancreatic beta cells > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, March 10, 2009, 2:34 PM > > Can I please get some suggestions for histological stains that > demonstrate pancreatic beta cells? I need something that would be highly > specific without using an IHC method. > > Any advice is greatly appreciated! > > > This communication is intended solely for the use of the addressee and may > contain information that is legally privileged, confidential or exempt > from disclosure. If you are not the intended recipient, please note that > any dissemination, distribution, or copying of this communication is > strictly prohibited. Anyone who receives this message in error should > notify the sender immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From azhisto <@t> hotmail.com Tue Mar 10 15:22:30 2009 From: azhisto <@t> hotmail.com (azhisto Histology) Date: Tue Mar 10 15:22:35 2009 Subject: [Histonet] Need Histologists ASAP Message-ID: New private AP lab in Phoenix, AZ., Top pay, great benefits, great hours, it's a new GI lab with state of the art equipment, please do not reply to this email, To submit a resume please apply to twincrest@bex.net _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From pritchm <@t> ccf.org Tue Mar 10 15:27:00 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Tue Mar 10 15:27:20 2009 Subject: [Histonet] Sirius red - staining specificity Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021513D6@CCHSCLEXMB56.cc.ad.cchs.net> Hello: Recently, I have used Sirius red (using the Kiernan Method found in Histological and Histochemical Methods, 4th Ed. Scion Publishing Ltd. 2008) to localize collagen fibers in livers of mice exposed to carbon tetrachloride over 5 weeks. Unfortunately, I do not have the capability to examine my slides using circular polarizing light microscopy at present, so I cannot determine at the present time whether or not the staining I observe is that of fibrillar collagens or not. I have a question: Is Sirius red (using the above method) able to stain laminin fibers? These data have lead me to develop some exciting, new hypotheses, so I have not yet had the time to research laminin biochemistry to try to answer this question myself. In case you are interested in a bit more background info read the following: My slides show remarkable differences in Sirius red staining depending on from which of my experimental groups the sections came. Specifically, I have noted a peculiarly diffuse Sirius red staining in livers of my carbon tetrachloride-exposed knockout mice while my carbon tetrachloride-exposed wild type mice have 'normal' Sirius red staining. Because of a series of other assays I have performed on these livers (except laminin IHC... I was trying to avoid optimizing a new stain before I had sufficient reason to do so), I suspect that the at much of the diffuse Sirius red staining I have observed in my knockout mice may reflect the presence of more laminin than collagen in the fibrotic livers from knockout mice, and therefore, may explain the odd pattern of ECM deposition. Any thoughts? I thank you, in advance, for your expert advice. -->mtp Michele T. Pritchard, Ph.D. Research Associate-Staff Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From fearj <@t> bronsonhg.org Tue Mar 10 15:45:50 2009 From: fearj <@t> bronsonhg.org (Joseph Fear) Date: Tue Mar 10 15:46:05 2009 Subject: [Histonet] validation documentation for processors Message-ID: <49B6993E020000EE00048A60@GWISE3.bronsonhg.org> A question came up in our lab about validation documentation for our new processor. Does anyone have any creative feedback on how your lab documents validation of machines like processors and stainers? See below----> >>>Joseph Fear 03/01/09>>> I'll post the question at histonet and see what i can find out about how other histo labs document validation of thier machines. I think with the peloris we ran test cases and Dr.Pearson checked the H&E slides, but you're right that we don't have documentation of what cases were run and with JP's signiture for approval of validation. I can work on a general 'machine validation form' in excel and get back with you. -Joseph >>> Virginia Rupert 02/20/09 3:44 PM >>> In your searches, or past experience, do you know or can you find out how new instruments are validated? I don't think we have adequate documentation for the Peloris, with test cases, etc. But I also haven't found a document to use as a template for our purposes. Any ideas? Thanks From rjbuesa <@t> yahoo.com Tue Mar 10 16:23:02 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 10 16:23:06 2009 Subject: [Histonet] validation documentation for processors In-Reply-To: <49B6993E020000EE00048A60@GWISE3.bronsonhg.org> Message-ID: <153792.12080.qm@web65703.mail.ac4.yahoo.com> As all validations, you will have to process at least 25 pieces (CAP requires from 25 to 100)?of tissue using your old and your new processor simultaneously. Make slides and later prepare H&E and?some HC and ?IHC procedures?using both sets of slides and give them to as many pathologists as you can so they can select, without knowing which section comes from which processor, with only two options: either one section is better than the other, or both are equivalent for diagnostic purposes. Later your data should be analyzed statistically with the chi-square test or you could obviate the test if almost (more than 90%) of all the pairs are qualified as equivalent. Ren? J. --- On Tue, 3/10/09, Joseph Fear wrote: From: Joseph Fear Subject: [Histonet] validation documentation for processors To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 4:45 PM A question came up in our lab about validation documentation for our new processor. Does anyone have any creative feedback on how your lab documents validation of machines like processors and stainers? See below----> >>>Joseph Fear 03/01/09>>> I'll post the question at histonet and see what i can find out about how other histo labs document validation of thier machines. I think with the peloris we ran test cases and Dr.Pearson checked the H&E slides, but you're right that we don't have documentation of what cases were run and with JP's signiture for approval of validation. I can work on a general 'machine validation form' in excel and get back with you. -Joseph >>> Virginia Rupert 02/20/09 3:44 PM >>> In your searches, or past experience, do you know or can you find out how new instruments are validated? I don't think we have adequate documentation for the Peloris, with test cases, etc. But I also haven't found a document to use as a template for our purposes. Any ideas? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs <@t> yahoo.com Tue Mar 10 18:15:23 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Tue Mar 10 18:15:30 2009 Subject: [Histonet] Cutting angle? Message-ID: <610222.16229.qm@web46110.mail.sp1.yahoo.com> Hello Listers, I have inherited a Reichert-Jung 2030 microtome. The knife holder is a bit funky and hard to set the clearance angle. What angle do y'all use? I'm trying to get 4 or 5 degrees. Thanks, Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From jshea121 <@t> roadrunner.com Tue Mar 10 20:09:07 2009 From: jshea121 <@t> roadrunner.com (Shea's) Date: Tue Mar 10 20:09:11 2009 Subject: [Histonet] CPT Coding question 88342 PIN 4 cocktail Message-ID: <545F6B6F5E6B46309E6074BDE1955D69@DJWYVL31> We started staining prostate needle bxs w/ PIN 4 (triple stain). 1. My understanding is that we can bill for 88342 x 3 (per specimen) if a comment is made on the results of the nuclear staining and cytoplasmic staining of the DAB and the staining of the Vulcan red. The key is documentation in the report. This is simple to understand when you rec'd 1 specimen/container, but we rec'd: A. Right prostate Bx - Red stained bx: apex Green stained bx: base Blue stained bx: midgland Yellow stained bx: transition submitted in one Cassette A. B. Left prostate Bx - Red stained bx: apex Green stained bx: base Blue stained bx: midgland Yellow stained bx: transition submitted in one Cassette B. 2. If the pathologist needed this stain on every specimen, it would be 8 separate identifiable specimens x 3 (separate identifiable stains in the cocktail) , therefore 88342 x 24, even though it is only 2 blocks. 3. If the pathologist was only interested in this stain on on the Apex in A and B it would be 2 specimens x 3, therefore 88342 x 6 for the same 2 blocks (even though it stains all 8 specimens, only 2 of the 8 are in need of this stain). Another words, we can't charge the technical component until we find out how many specimens the pathologist is looking at in a slide (professional component), even though it is the same amount of work and reagents. Does this sound right? 4. Is the technical charge always the same as the professional charge? Another unrelated question - We can bill for decalcification, but is there a billing code for KOH in the same manner to soften and treat toenails before processing? It is documented in the Path report. I know this has been discussed in the archives, but there seems to be conflicting opinions. Do we know for sure? Thanks From yvan_lindekens <@t> yahoo.com Tue Mar 10 23:42:39 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Mar 10 23:42:47 2009 Subject: [Histonet] User manual for Shandon HistoCenter 2 Message-ID: <554284.50072.qm@web110203.mail.gq1.yahoo.com> If you have one (or a xerox copy) to spare I would be very, very, very obliged. Thanks in advance! Yvan. From SharonC <@t> celligent.net Wed Mar 11 03:56:46 2009 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Wed Mar 11 03:56:53 2009 Subject: [Histonet] slide disposal Message-ID: Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net From mpence <@t> grhs.net Wed Mar 11 08:21:55 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Mar 11 08:22:03 2009 Subject: [Histonet] slide disposal In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3AD5@IS-E2K3.grhs.net> All of our slides are put into buckets and placed in the regular dumpster as long as there is no patient names on the slides. We don't even treat them as "sharps". Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 3:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Mar 11 08:34:58 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 11 08:35:08 2009 Subject: [Histonet] Cutting angle? In-Reply-To: <610222.16229.qm@web46110.mail.sp1.yahoo.com> References: <610222.16229.qm@web46110.mail.sp1.yahoo.com> Message-ID: <454783E32EF08EE99EFF042B@bchwxp2702.ad.med.buffalo.edu> I have a refurbished Reichert-Jung 2030, but with a knife holder from a newer model (so I'm told by the refurbisher). I use 0-1 degree angle. Merced --On Tuesday, March 10, 2009 4:15 PM -0700 Va Paula Sicurello wrote: > > Hello Listers, > > I have inherited a Reichert-Jung 2030 microtome. The knife holder is a > bit funky and hard to set the clearance angle. > > What angle do y'all use? I'm trying to get 4 or 5 degrees. > > Thanks, > > Paula :-) > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Kristopher.Kalleberg <@t> unilever.com Wed Mar 11 08:40:18 2009 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Wed Mar 11 08:40:23 2009 Subject: [Histonet] incubator oven Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C05B5BAFF@NTRSEVS30002.s3.ms.unilever.com> Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted before the processing of the slides. I will be using this oven for strictly melting paraffin containers to have ready for embedding center and for baking of slides to melt paraffin prior to processing. Has anyone had any concerns or issues with these new convection ovens or can they recommend a decent oven to buy. it just needs to be a medium sized oven. Thanks in advance. Kris kalleberg kristopher.kalleberg@unilever.com From leiker <@t> buffalo.edu Wed Mar 11 08:41:54 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 11 08:42:05 2009 Subject: [Histonet] slide disposal In-Reply-To: References: Message-ID: <57994724AEB27F2E0A637905@bchwxp2702.ad.med.buffalo.edu> We have a "broken glass" waste container we put ours in for pick up and disposal (don't deal with non-animal patients, so not an issue). -M --On Wednesday, March 11, 2009 4:56 AM -0400 Sharon Campbell wrote: > Hi everyone! > > Thank you for all the great responses to my last question about metal > vs. plastic molds. I have another question being debated however, How to > dispose of slides once the required time is up (10 years for us). We > have put the slide in sharps containers and then into biohazard, but are > they really bioharzard, the slides only contain the patients accession > number - no personal ID on the slides? How do you guys dispose of your > slides when space and money are an issue? > > Thank you > > > > Sharon Campbell, HTL(ASCP)CM, BSBM > > Histology Supervisor > > Celligent Diagnostics, LLC > > Formerly Pathology Associates Services > > 101 East W.T. Harris Blvd, Suite 1212 > > Charlotte, NC 28262 > > 800-524-6779 ext. 104 > > 704-970-3304 Direct Line > > sharonc@celligent.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From mcauliff <@t> umdnj.edu Wed Mar 11 08:49:19 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Mar 11 08:48:04 2009 Subject: [Histonet] histological stain for pancreatic beta cells In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B94@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A65D8B94@wave-mail.7thwave.local> Message-ID: <49B7C15F.3070608@umdnj.edu> Aldehyde fuchsin! Much simpler than Mallory Azan and very specific. You will need paraldehyde, a controlled substance, to make this stain. Alternatively, you can use acetaldehyde but you will need to triple the amount to get the molar equivalents correct. See "Staining properties of Aldehyde fuchsin analogues" by Buehner, Nettleton and Longley, J. Histochem. Cytochem. 27(3):782-787, 1979. Geoff Michele Wich wrote: > Can I please get some suggestions for histological stains that > demonstrate pancreatic beta cells? I need something that would be highly > specific without using an IHC method. > > Any advice is greatly appreciated! > > > This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Wed Mar 11 09:19:38 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 09:19:53 2009 Subject: [Histonet] slide disposal In-Reply-To: Message-ID: <689175.22454.qm@web65702.mail.ac4.yahoo.com> Old slides, after all the processing they have "endured" are not hazardous (from the contagious/disease point of view) any more. They are hazardous from the mechanical (potential physical injury?point of view) and it is more than enough to dispose of them in sharps containers. Word of caution, just do not dump the slides on the sharp containers because they will occupy much more space (and will require more sharp containers) as if you try to place?them in a?good arrangement. Do not succumb to the temptation of making noise with the old slides as yiu dispose of them! Ren? J.? --- On Wed, 3/11/09, Sharon Campbell wrote: From: Sharon Campbell Subject: [Histonet] slide disposal To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 4:56 AM Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 11 09:22:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 09:22:54 2009 Subject: [Histonet] incubator oven In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C05B5BAFF@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <321533.23070.qm@web65708.mail.ac4.yahoo.com> Usually all ovens (new and old are of the convection type) and the air circulation will improve the effect of heat over the slides. Ren? J. --- On Wed, 3/11/09, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] incubator oven To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 9:40 AM Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted before the processing of the slides. I will be using this oven for strictly melting paraffin containers to have ready for embedding center and for baking of slides to melt paraffin prior to processing. Has anyone had any concerns or issues with these new convection ovens or can they recommend a decent oven to buy. it just needs to be a medium sized oven. Thanks in advance. Kris kalleberg kristopher.kalleberg@unilever.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonetalias <@t> gmail.com Wed Mar 11 09:24:55 2009 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Wed Mar 11 09:25:04 2009 Subject: [Histonet] metal molds vs. disposable molds In-Reply-To: <58764FED08812DF8952DFD90@bchwxp2702.ad.med.buffalo.edu> References: <5D64396A0D4A5346BEBC759022AAEAA53AC231@ITSSSXM01V6.one.ads.che.org> <58764FED08812DF8952DFD90@bchwxp2702.ad.med.buffalo.edu> Message-ID: <4b6c85510903110724n62ded0b9wf169ec63159c04fb@mail.gmail.com> I have had issues with the plastic molds leaving an indention or not leaving a flat cutting surface. This is not good when you are cutting prostate biopsies. The ends of the tissue get cut away before you get to the center of the block. After seeing a tech do this I immediately trashed the plastic. Even though I would prefer plastic because it is easier for my lab I can not risk losing patient tissue. On Tue, Mar 10, 2009 at 1:36 PM, Merced Leiker wrote: > ...which might be the better thing to do then...since it'll all be floating > on the surface anyway and we don't want to risk clogging drain pipes with > the wax once it cools... > > > --On Tuesday, March 10, 2009 12:59 PM -0400 "Weems, Joyce" < > JWeems@sjha.org> wrote: > > And if you let the water cool, the paraffin will harden and you discard >> it without pouring down the drain... >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced >> Leiker >> Sent: Tuesday, March 10, 2009 12:22 PM >> To: Sharon Campbell; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] metal molds vs. disposable molds >> >> I like my metal molds. They are neat, you get even heat transfer, and >> are easy to clean when they need it. I clean them by tossing them in >> some boiling water with an excess of cleaning powder (Alconox or >> Sparkleen) or any detergent you have on hand (make sure it doesn't get >> too bubbly and froth over though). Then I rinse them under hot top >> water, spread on paper towels to dry, spray with mold release, and stack >> in the oven. >> >> Merced >> >> --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell >> wrote: >> >> Hello everyone, >>> >>> We have a debate going on about purchasing metal molds vs. disposable >>> plastic molds. Which is better? Is the cost better in the long run to >>> buy metal? Also, how does everyone clean their metal molds? >>> >>> Thanks >>> >>> >>> >>> Sharon Campbell, HTL(ASCP)CM, BSBM >>> >>> Histology Supervisor >>> >>> Celligent Diagnostics, LLC >>> >>> Formerly Pathology Associates Services >>> >>> 101 East W.T. Harris Blvd, Suite 1212 >>> >>> Charlotte, NC 28262 >>> >>> 800-524-6779 ext. 104 >>> >>> 704-970-3304 Direct Line >>> >>> sharonc@celligent.net >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> >> Merced M Leiker >> Research Technician II >> 354 Biomedical Research Building >> School of Medicine and Biomedical Sciences State University of New York >> at Buffalo >> 3435 Main St, Buffalo, NY 14214 >> Ph: (716) 829-6033 >> Fx: (716) 829-2725 >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This email, including any attachments is the >> property of Catholic Health East and is intended >> for the sole use of the intended recipient(s). >> It may contain information that is privileged and >> confidential. Any unauthorized review, use, >> disclosure, or distribution is prohibited. If you are >> not the intended recipient, please reply to the >> sender that you have received the message in >> error, then delete this message. >> >> >> > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From vjp2105 <@t> columbia.edu Wed Mar 11 09:48:12 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Wed Mar 11 09:48:18 2009 Subject: [Histonet] IF on frozen sections Message-ID: Hi everyone, Does anyone have any experience in IF on mouse embryo frozen sections? Vanessa From tbraud <@t> holyredeemer.com Wed Mar 11 09:53:27 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Mar 11 09:53:38 2009 Subject: [Histonet] RE: CAP regs on Histonet In-Reply-To: <682a2d1300006038@HolyRedeemer.com> Message-ID: For ease of reference, if you are asking, replying, or quoting anything pertaining to CAP regs, PLEASE give the CAP checklist number that contains the information being discussed. For those of us trying to keep current, it makes it so much easier to check our own records. Just a suggestion - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From dellav <@t> musc.edu Wed Mar 11 10:01:00 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Mar 11 10:01:04 2009 Subject: [Histonet] CPT Coding question 88342 PIN 4 cocktail In-Reply-To: <545F6B6F5E6B46309E6074BDE1955D69@DJWYVL31> References: <545F6B6F5E6B46309E6074BDE1955D69@DJWYVL31> Message-ID: Disclaimer: I do not consider myself to be a coding expert. Take my opinions with a grain of salt. I've never seen coding for softening of keratin therefore I don't see how you could charge for it. This is not decalcification and should not be represented as such. Until such time as a code appears (don't hold your breath) the KOH or whatever technique you employ is for your convenience to make a block more cuttable (is that a word?). There is no rule that technical and professional charges must be the same. They are reimbursed differently, sometimes the technical having a higher reimbursement than the professional. You are free to set your charges for each where you see fit. I believe that your charges for prostate IHC have to be justified by the pathology. In your example, some of those cores may be clearly benign and there would be no basis for charging IHC for eight cores if only one or two contain the lesion. In your case, by having four cores in one block, you benefit by keeping your expenses down however I do believe that your IHC charges should (must?) be based on the location of the lesion under study. It would seem to me that charging for IHC on eight cores when the report describes a lesion in only one is asking for trouble You are correct that you can charge 88342 x 3 for the PIN 4 markers as each is separate and can be visualized distinctly from one another. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: Tuesday, March 10, 2009 9:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT Coding question 88342 PIN 4 cocktail We started staining prostate needle bxs w/ PIN 4 (triple stain). 1. My understanding is that we can bill for 88342 x 3 (per specimen) if a comment is made on the results of the nuclear staining and cytoplasmic staining of the DAB and the staining of the Vulcan red. The key is documentation in the report. This is simple to understand when you rec'd 1 specimen/container, but we rec'd: A. Right prostate Bx - Red stained bx: apex Green stained bx: base Blue stained bx: midgland Yellow stained bx: transition submitted in one Cassette A. B. Left prostate Bx - Red stained bx: apex Green stained bx: base Blue stained bx: midgland Yellow stained bx: transition submitted in one Cassette B. 2. If the pathologist needed this stain on every specimen, it would be 8 separate identifiable specimens x 3 (separate identifiable stains in the cocktail) , therefore 88342 x 24, even though it is only 2 blocks. 3. If the pathologist was only interested in this stain on on the Apex in A and B it would be 2 specimens x 3, therefore 88342 x 6 for the same 2 blocks (even though it stains all 8 specimens, only 2 of the 8 are in need of this stain). Another words, we can't charge the technical component until we find out how many specimens the pathologist is looking at in a slide (professional component), even though it is the same amount of work and reagents. Does this sound right? 4. Is the technical charge always the same as the professional charge? Another unrelated question - We can bill for decalcification, but is there a billing code for KOH in the same manner to soften and treat toenails before processing? It is documented in the Path report. I know this has been discussed in the archives, but there seems to be conflicting opinions. Do we know for sure? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KKay <@t> chr.ab.ca Wed Mar 11 10:35:20 2009 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Wed Mar 11 10:35:29 2009 Subject: [Histonet] MOLDS AND SLIDE DISPOSAL In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494F02@exbe.chr.ab.ca> Metal molds vs Disposable molds We too have chosen metal molds. We clean them by placing them in a random processing basket and running an extended purge cycle with xylene and alcohol. It has worked very well for us. Slide disposal: I believe the CAP guidelines from 2005 state that slides should be kept for a minimum of 20 years. I don't know if this has been recently revised. Here in Alberta, Canada, the retention guidelines for slides is 30 years.(College of Physicians and Surgeons of Alberta) We also package into plastic pails to be picked up by a disposal company. They are never put into a regular dumpster as there is definite danger of slide breakage and potential injury to anyone who comes in contact with them. I realize that this is an added expense however, with a longer retention time, the slides are not discarded as often. Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory - Chinook Regional Hospital 960 - 19 st South,Lethbridge, Alberta,CANADA (403) 388 - 6061 (Phone) Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 3:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net - This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From smcbride <@t> cs.cmu.edu Wed Mar 11 11:30:10 2009 From: smcbride <@t> cs.cmu.edu (Sean McBride) Date: Wed Mar 11 11:30:18 2009 Subject: [Histonet] Need Advice on Low Temp Tissue Processing Message-ID: Hi folks, I have a rabbit cranial defect study in which the implant material is sensitive to 100% ethanol at temperatures > 20*C. As I cannot use my my Sakura tissue processor for the specimens (min operating temp=35*C), I plan to process them by hand @ 4*C in a cold room. My intuition tells me that at lower temperatures, the processing times in each solution should be extended by a percentage, but by how much, I have not an idea. Does anyone have any experience or ideas with regards to low temperature tissue processing times? Best regards, ~Sean McBride Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641 (fax) smcbride@cs.cmu.edu From vapatpxs <@t> yahoo.com Wed Mar 11 11:37:21 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Mar 11 11:37:25 2009 Subject: [Histonet] incubator oven Message-ID: <688227.56779.qm@web46113.mail.sp1.yahoo.com> Hi Kris, I think that a smallish lab oven would suffice. If all you are doing is melting paraffins I think any of the ones from the major vendors (Fisher, VWR, etc.) would be good enough. I do believe that those are still just ovens and don't have the air currents. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/11/09, Kalleberg, Kristopher wrote: > From: Kalleberg, Kristopher > Subject: [Histonet] incubator oven > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, March 11, 2009, 1:40 PM > Hi All, > > I desperately need to get a new incubator oven for my > histology lab.? It > seems as if most ovens are now convection ovens.? > Since my old oven is > not convection I am just concerned that the constant air > movement will > some how affect the tissue slides in the way the paraffin > in melted > before the processing of the slides.? I will be using > this oven for > strictly melting paraffin containers to have ready for > embedding center > and for baking of slides to melt paraffin prior to > processing.? Has > anyone had any concerns or issues with these new convection > ovens or can > they recommend a decent oven to buy.? it just needs to > be a medium sized > oven.? > Thanks in? advance. > > Kris kalleberg > kristopher.kalleberg@unilever.com > ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcline <@t> wchsys.org Wed Mar 11 11:51:14 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Mar 11 11:51:19 2009 Subject: [Histonet] slide disposal In-Reply-To: Message-ID: <95F8D7F50A7D4B32A824EAD771BD14E8@wchsys.org> We use the same company that takes away our alcohol waste. They pulverize the slides for us. There is a company in WI that makes a Slydeater that you can rent for one month to pulverize your slides. I am looking into that aspect. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rjbuesa <@t> yahoo.com Wed Mar 11 12:08:13 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 12:08:23 2009 Subject: [Histonet] Need Advice on Low Temp Tissue Processing In-Reply-To: Message-ID: <13274.14142.qm@web65716.mail.ac4.yahoo.com> Sean: Considering that the van't Hoff coefficient determines a metabolic coefficient increase/reduction every 10?C (Q10) I would?increase the processing period?one?time every 10?c between the temperature your protocol was designed to work at and the new temperature you are going to use now. Lets say that you fine tuned your protocol for 35 ?C and now you will be working at 4?C which is a almost 30?C less = 10?C x 3 If a given step at 35?C was set at 1 hour, I would use now 3 hours for the same step. Ren? J. --- On Wed, 3/11/09, Sean McBride wrote: From: Sean McBride Subject: [Histonet] Need Advice on Low Temp Tissue Processing To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 12:30 PM Hi folks, I have a rabbit cranial defect study in which the implant material is sensitive to 100% ethanol at temperatures > 20*C. As I cannot use my my Sakura tissue processor for the specimens (min operating temp=35*C), I plan to process them by hand @ 4*C in a cold room. My intuition tells me that at lower temperatures, the processing times in each solution should be extended by a percentage, but by how much, I have not an idea. Does anyone have any experience or ideas with regards to low temperature tissue processing times? Best regards, ~Sean McBride Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641 (fax) smcbride@cs.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drvet_anjan <@t> hotmail.com Wed Mar 11 12:15:35 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Wed Mar 11 12:15:41 2009 Subject: [Histonet] paraffin oil-clearing agent Message-ID: hi everyone, last week i had aquestion regarding xylene substitutes and Vector Blue compatibility..... i came across an article saying use of paraffin oil as substitute for xylene....... can paraffin oil be used as clearing agent ? _________________________________________________________________ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx From Lynne.Bell <@t> cvmc.org Wed Mar 11 12:17:31 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Wed Mar 11 12:17:35 2009 Subject: [Histonet] Compromised specimens Message-ID: When our histology lab receives a "compromised specimen" (not labeled with patient's name, wrong DOB, no label whatsoever, etc) we have the offending party correct the error and fill out an accountability form accepting full responsibility for the identification of the specimen. We then scan the accountability form into the patient's medical record and include the information in the "specimen comments" section of the pathology record. This information does not print on the official pathology report. My question - should the pathologist include in his/her report the compromised specimen information? Presently, on our clinical lab reports and cytology reports it is included - simply saying "Compromised Specimen". Our pathologists historically have not wanted to include this in the official pathology report and I feel that it should be in there - sort of a "cover your butt" addendum. I'm curious what other hospitals do!! Thanks in advance, Lynne A. Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From tifei <@t> foxmail.com Wed Mar 11 12:40:13 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Mar 11 12:40:34 2009 Subject: [Histonet] slide disposal References: <661949901A768E4F9CC16D8AF8F2838C017A3AD5@IS-E2K3.grhs.net> Message-ID: <200903120140083369900@foxmail.com> d2UgdHJlYXQgd2l0aCBTSEFSUFMgKyBCaW9sb2dpY2FsIEhhemFyZG91cw0KDQoNCjIwMDktMDMt MTIgDQoNCg0KDQpURiANCg0KDQoNCreivP7Iy6O6IE1pa2UgUGVuY2UgDQq3osvNyrG85KO6IDIw MDktMDMtMTEgIDIxOjI5OjI5IA0KytW8/sjLo7ogU2hhcm9uIENhbXBiZWxsOyBoaXN0b25ldEBs aXN0cy51dHNvdXRod2VzdGVybi5lZHUgDQqzrcvNo7ogDQrW98zio7ogUkU6IFtIaXN0b25ldF0g c2xpZGUgZGlzcG9zYWwgDQogDQpBbGwgb2Ygb3VyIHNsaWRlcyBhcmUgcHV0IGludG8gYnVja2V0 cyBhbmQgcGxhY2VkIGluIHRoZSByZWd1bGFyDQpkdW1wc3RlciBhcyBsb25nIGFzIHRoZXJlIGlz IG5vIHBhdGllbnQgbmFtZXMgb24gdGhlIHNsaWRlcy4gV2UgZG9uJ3QNCmV2ZW4gdHJlYXQgdGhl bSBhcyAic2hhcnBzIi4gDQpNaWtlDQotLS0tLU9yaWdpbmFsIE1lc3NhZ2UtLS0tLQ0KRnJvbTog aGlzdG9uZXQtYm91bmNlc0BsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNClttYWlsdG86aGlzdG9u ZXQtYm91bmNlc0BsaXN0cy51dHNvdXRod2VzdGVybi5lZHVdIE9uIEJlaGFsZiBPZiBTaGFyb24N CkNhbXBiZWxsDQpTZW50OiBXZWRuZXNkYXksIE1hcmNoIDExLCAyMDA5IDM6NTcgQU0NClRvOiBo aXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNClN1YmplY3Q6IFtIaXN0b25ldF0gc2xp ZGUgZGlzcG9zYWwNCkhpIGV2ZXJ5b25lIQ0KVGhhbmsgeW91IGZvciBhbGwgdGhlIGdyZWF0IHJl c3BvbnNlcyB0byBteSBsYXN0IHF1ZXN0aW9uIGFib3V0IG1ldGFsDQp2cy4gcGxhc3RpYyBtb2xk cy4gSSBoYXZlIGFub3RoZXIgcXVlc3Rpb24gYmVpbmcgZGViYXRlZCBob3dldmVyLCBIb3cgdG8N CmRpc3Bvc2Ugb2Ygc2xpZGVzIG9uY2UgdGhlIHJlcXVpcmVkIHRpbWUgaXMgdXAgKDEwIHllYXJz IGZvciB1cykuIFdlDQpoYXZlIHB1dCB0aGUgc2xpZGUgaW4gc2hhcnBzIGNvbnRhaW5lcnMgYW5k IHRoZW4gaW50byBiaW9oYXphcmQsIGJ1dCBhcmUNCnRoZXkgcmVhbGx5IGJpb2hhcnphcmQsIHRo ZSBzbGlkZXMgb25seSBjb250YWluIHRoZSBwYXRpZW50cyBhY2Nlc3Npb24NCm51bWJlciAtIG5v IHBlcnNvbmFsIElEIG9uIHRoZSBzbGlkZXM/IEhvdyBkbyB5b3UgZ3V5cyBkaXNwb3NlIG9mIHlv dXINCnNsaWRlcyB3aGVuIHNwYWNlIGFuZCBtb25leSBhcmUgYW4gaXNzdWU/DQpUaGFuayB5b3UN Cg0KU2hhcm9uIENhbXBiZWxsLCBIVEwoQVNDUClDTSwgQlNCTQ0KSGlzdG9sb2d5IFN1cGVydmlz b3INCkNlbGxpZ2VudCBEaWFnbm9zdGljcywgTExDDQpGb3JtZXJseSBQYXRob2xvZ3kgQXNzb2Np YXRlcyBTZXJ2aWNlcw0KMTAxIEVhc3QgVy5ULiBIYXJyaXMgQmx2ZCwgU3VpdGUgMTIxMg0KQ2hh cmxvdHRlLCBOQyAgMjgyNjINCjgwMC01MjQtNjc3OSBleHQuIDEwNA0KNzA0LTk3MC0zMzA0IERp cmVjdCBMaW5lDQpzaGFyb25jQGNlbGxpZ2VudC5uZXQNCg0KX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX18NCkhpc3RvbmV0IG1haWxpbmcgbGlzdA0KSGlzdG9u ZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4u ZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQNCl9fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxp c3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9t YWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo= From tifei <@t> foxmail.com Wed Mar 11 12:41:56 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Mar 11 12:42:17 2009 Subject: =?utf-8?B?UmU6IFJFOiBbSGlzdG9uZXRdIENEMzEgb24gcmF0IGJyYWlu?= References: <69CF6991-6EB5-4645-8A95-442EC8E9BCCC@bioacademy.gr>, <200903052131230910571@foxmail.com>, <49B00474.2070909@umn.edu>, <200903062322352578736@foxmail.com>, <87EF241A3ACD47489DC61AEE79BC7D9ECBA7DC@MSGWSDCPMB01.nyumc.org> Message-ID: <200903120141515622641@foxmail.com> hi, i am trying to stain the rat CD31 the cat: 550274 is the anti-mouse CD31 have anyone tried this one: http://www.bdbiosciences.com/external_files/pm/doc/tds/rat/live/web_enabled/22711D_555025.pdf test it on parafin sections? 2009-03-12 TF ???? Chiriboga, Luis ????? 2009-03-07 00:16:34 ???? tifei@foxmail.com; Colleen Forster ??? histonet; ????? ????????? ??? RE: [Histonet] CD31 on rat brain For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). can supply more info if need. The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TF Sent: Friday, March 06, 2009 10:23 AM To: Colleen Forster Cc: histonet; ????? ????????? Subject: [Histonet] CD31 on rat brain Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF ???? Colleen Forster ????? 2009-03-06 00:57:29 ???? tifei ??? iskaliora; histonet; ????? ????????? ??? Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > ???? iskaliora > ????? 2009-03-05 18:49:11 > ???? histonet > ??? ????? ????????? > ??? [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C? (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskaliora@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From mark <@t> vyleater.com Wed Mar 11 13:29:22 2009 From: mark <@t> vyleater.com (Mark J. Griffith) Date: Wed Mar 11 13:29:36 2009 Subject: [Histonet] slide disposal Message-ID: <20090311182925.EEBT26415.outaamta01.mail.tds.net@marklt.ramflat.com> Thank you Joyce - The SlydEater pulverizes the glass slide and obliterates the label affixed to the slide. Units are available for both short term and long term rentals, as well as Least to Own. If interested, visit our website at: http://www.SlydEater.com or by calling me directly at 800-233-3721. Mark J. Griffith E-mail: mailto:mark@vyleater.com S & G Enterprises, Inc. N115 W19000 Edison Drive Germantown, WI 53022 USA Tel: 262/251-8300 US/Canada toll-free: 800/233-3721 Fax: 262/251-1616 >From: "Joyce Cline" >To: "Histonet" >Date: Wed, 11 Mar 2009 12:51:14 -0400 > >We use the same company that takes away our alcohol waste. They pulverize >the slides for us. There is a company in WI that makes a Slydeater that you >can rent for one month to pulverize your slides. I am looking into that >aspect. > >Joyce Cline, H.T. (ASCP) >Technical Specialist >Hagerstown Medical Lab. >301-665-4980 >fax 301-665-4941 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon >Campbell >Sent: Wednesday, March 11, 2009 4:57 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] slide disposal > >Hi everyone! > >Thank you for all the great responses to my last question about metal >vs. plastic molds. I have another question being debated however, How to >dispose of slides once the required time is up (10 years for us). We >have put the slide in sharps containers and then into biohazard, but are >they really bioharzard, the slides only contain the patients accession >number - no personal ID on the slides? How do you guys dispose of your >slides when space and money are an issue? > >Thank you > > > >Sharon Campbell, HTL(ASCP)CM, BSBM > >Histology Supervisor > >Celligent Diagnostics, LLC > >Formerly Pathology Associates Services > >101 East W.T. Harris Blvd, Suite 1212 > >Charlotte, NC 28262 > >800-524-6779 ext. 104 > >704-970-3304 Direct Line > >sharonc@celligent.net From vapatpxs <@t> yahoo.com Wed Mar 11 13:29:53 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Mar 11 13:29:56 2009 Subject: [Histonet] Bad sections Message-ID: <933464.47492.qm@web46113.mail.sp1.yahoo.com> Hi Histo netters, I am am my wit's end. I know how to section but my sections are compressing like crazy. I've altered my processing protocol thinking that I was overprocessing my samples. I've tried different knife angles, different brands of razor blade knives. It's either me or the microtome. Info- I process animal tissues with between 30-60 minute steps. Vacuum paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish degrees (a little below the middle line on the knife holder). Soak blocks on Downey ice blocks, water bath at 44 degrees. Suggestions? Waa, Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From lchen <@t> mednet.ucla.edu Wed Mar 11 13:53:07 2009 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Wed Mar 11 13:53:12 2009 Subject: [Histonet] detergent In-Reply-To: <361429220903111152q5b1ab3a9m20ee1d1369683738@mail.gmail.com> References: <361429220903111152q5b1ab3a9m20ee1d1369683738@mail.gmail.com> Message-ID: <361429220903111153n1d9e61b7v35148b14b27e1d59@mail.gmail.com> Hi Rene, To clarify your post: Do you mean liquid dish soap (like palmolive) is to be used? When you say not dishwasher detergent I think of Automatic Dishwasher detergent such as Cascade. Or no dish soap at all and I should use liquid laundry detergent? Thanks. Leslie From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. From leiker <@t> buffalo.edu Wed Mar 11 13:53:41 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 11 13:53:47 2009 Subject: [Histonet] Bad sections In-Reply-To: <933464.47492.qm@web46113.mail.sp1.yahoo.com> References: <933464.47492.qm@web46113.mail.sp1.yahoo.com> Message-ID: <2EDADD9D935B7C2045FD315D@bchwxp2702.ad.med.buffalo.edu> What animal tissues are you using? If rodent, may need less time on the steps - like 10-20 min. --On Wednesday, March 11, 2009 11:29 AM -0700 Va Paula Sicurello wrote: > > Hi Histo netters, > > I am am my wit's end. I know how to section but my sections are > compressing like crazy. I've altered my processing protocol thinking > that I was overprocessing my samples. > > I've tried different knife angles, different brands of razor blade knives. > > It's either me or the microtome. > > Info- I process animal tissues with between 30-60 minute steps. Vacuum > paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish > degrees (a little below the middle line on the knife holder). Soak blocks > on Downey ice blocks, water bath at 44 degrees. > > Suggestions? > > Waa, > > Paula > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From denise.woodward <@t> uconn.edu Wed Mar 11 14:04:07 2009 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Wed Mar 11 14:04:13 2009 Subject: [Histonet] IHC chromogen loss, HELP! Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E4FF@EXCHANGED.mgmt.ad.uconn.edu> Posting for a friend.......... "We have hit a bump in the road with our IHC test and thought maybe you might have some input as we seem to be stuck. We stain (typically for virus) frozen bovine tissues using Biocare's Mach-3 AP polymer kit and develop with Vector Red, counterstain, and dehydrate and mount with Histoclear/VectaMount by hand or xylene/Surgipath mountant on the autocoverslipper. We have been noticing a major loss of stain after development recently. The controls seem to remain stained, it's the more diffuse and minimally stained sections that we have seen stain disappear between development and coverslipping. I did a test with Impress-DAB and Impress-Bajoran Purple to compare and we thought we had solved the problem as being related to the surgipath mountant. However I just did a run with cell markers coverslipping with the Histoclear/Vectamount and there seems to be a loss of stain again. Vector suggested trying 200 mM TBS for wash/diluent/stop instead of 100 mM. This is a recent problem as we have been using this protocol successfully for a few years now. Any suggestions would be greatly appreciated!" Replies directly to Meghan.tucker@ARS.USDA.GOV would be appreciated From MLafrini <@t> csmlab.com Wed Mar 11 14:23:18 2009 From: MLafrini <@t> csmlab.com (Michael LaFriniere) Date: Wed Mar 11 14:23:36 2009 Subject: [Histonet] validation documentation for processors In-Reply-To: <153792.12080.qm@web65703.mail.ac4.yahoo.com> References: <49B6993E020000EE00048A60@GWISE3.bronsonhg.org> <153792.12080.qm@web65703.mail.ac4.yahoo.com> Message-ID: <49B7D755.588C.00AF.0@csmlab.com> Rene, I agree with your process as to validation, however, could you help me understand where does the CAP state (what check off list item) concerning validation requirements when changing out processors or processing technologies. My staff is currently performing the validation process with a large microwave processor. If CAP does have a recommendation I would appreciate seeing it. I have a (1) out of (12) pathologists that (I think is going overboard) who would like my Pathology Manager to perform over 100 tissue samples for validation process to include IHC/FISH/special staining as we make the switch from conventional to microwave processing..... Thanks! Michael Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 3/10/2009 at 5:23 PM, Rene J Buesa wrote: As all validations, you will have to process at least 25 pieces (CAP requires from 25 to 100) of tissue using your old and your new processor simultaneously. Make slides and later prepare H&E and some HC and IHC procedures using both sets of slides and give them to as many pathologists as you can so they can select, without knowing which section comes from which processor, with only two options: either one section is better than the other, or both are equivalent for diagnostic purposes. Later your data should be analyzed statistically with the chi-square test or you could obviate the test if almost (more than 90%) of all the pairs are qualified as equivalent. Ren? J. --- On Tue, 3/10/09, Joseph Fear wrote: From: Joseph Fear Subject: [Histonet] validation documentation for processors To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 4:45 PM A question came up in our lab about validation documentation for our new processor. Does anyone have any creative feedback on how your lab documents validation of machines like processors and stainers? See below----> >>>Joseph Fear 03/01/09>>> I'll post the question at histonet and see what i can find out about how other histo labs document validation of thier machines. I think with the peloris we ran test cases and Dr.Pearson checked the H&E slides, but you're right that we don't have documentation of what cases were run and with JP's signiture for approval of validation. I can work on a general 'machine validation form' in excel and get back with you. -Joseph >>> Virginia Rupert 02/20/09 3:44 PM >>> In your searches, or past experience, do you know or can you find out how new instruments are validated? I don't think we have adequate documentation for the Peloris, with test cases, etc. But I also haven't found a document to use as a template for our purposes. Any ideas? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From burch007 <@t> mc.duke.edu Wed Mar 11 14:40:54 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Wed Mar 11 14:41:13 2009 Subject: [Histonet] HTLV-1 IHC Message-ID: Is anyone performing IHC on FFPE human tissue for HTLV-1? Many Thanks, Jim From rjbuesa <@t> yahoo.com Wed Mar 11 14:42:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 14:43:02 2009 Subject: [Histonet] paraffin oil-clearing agent In-Reply-To: Message-ID: <28188.44208.qm@web65714.mail.ac4.yahoo.com> Yes!!!!!! Paraffin oil = mineral oil. Under separate cover I am sending you an article I wrote on the subject. Ren? J. --- On Wed, 3/11/09, anjan kumar wrote: From: anjan kumar Subject: [Histonet] paraffin oil-clearing agent To: "triple immunohistochem" Date: Wednesday, March 11, 2009, 1:15 PM hi everyone, last week i had aquestion regarding xylene substitutes and Vector Blue compatibility..... i came across an article saying use of paraffin oil as substitute for xylene....... can paraffin oil be used as clearing agent ? _________________________________________________________________ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 11 14:47:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 14:47:13 2009 Subject: [Histonet] Compromised specimens In-Reply-To: Message-ID: <560905.95109.qm@web65715.mail.ac4.yahoo.com> As long as everything is solved and there is no doubt about whose specimen it is, I don't see any benefit in letting people know that is was initially compromised. Ren? J. --- On Wed, 3/11/09, Bell, Lynne wrote: From: Bell, Lynne Subject: [Histonet] Compromised specimens To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Wednesday, March 11, 2009, 1:17 PM When our histology lab receives a "compromised specimen" (not labeled with patient's name, wrong DOB, no label whatsoever, etc) we have the offending party correct the error and fill out an accountability form accepting full responsibility for the identification of the specimen. We then scan the accountability form into the patient's medical record and include the information in the "specimen comments" section of the pathology record. This information does not print on the official pathology report. My question - should the pathologist include in his/her report the compromised specimen information? Presently, on our clinical lab reports and cytology reports it is included - simply saying "Compromised Specimen". Our pathologists historically have not wanted to include this in the official pathology report and I feel that it should be in there - sort of a "cover your butt" addendum. I'm curious what other hospitals do!! Thanks in advance, Lynne A. Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 11 14:50:12 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 14:50:17 2009 Subject: [Histonet] Fw: Re: detergent Message-ID: <495962.45540.qm@web65714.mail.ac4.yahoo.com> Leslie: If you use Cascade, Palmolive or any other dishwasher liquid soap the paraffin will be dissolved and you will ruin your sections. I am referring to laundry liquid soap or mild hand washing liquid soap. Ren? J. --- On Wed, 3/11/09, Leslie Chen wrote: From: Leslie Chen Subject: detergent To: rjbuesa@yahoo.com Date: Wednesday, March 11, 2009, 2:52 PM Hi Rene, To clarify your post: Do you mean liquid dish soap (like palmolive) is to be used?? When you say not dishwasher detergent I think of Automatic Dishwasher detergent such as Cascade.? Or no dish soap at all and I should use liquid laundry detergent?? Thanks. Leslie From: Rene J Buesa > Subject: Re: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" , "Perry, Margaret" > Date: Wednesday, March 4, 2009, 9:14 PM > Add a few drops (4-5) of liquid > detergent, but not dishwasher detergent (because it will > dissolve the paraffin). > reni J. From rjbuesa <@t> yahoo.com Wed Mar 11 14:57:31 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 11 14:57:39 2009 Subject: [Histonet] Bad sections In-Reply-To: <933464.47492.qm@web46113.mail.sp1.yahoo.com> Message-ID: <177366.52269.qm@web65705.mail.ac4.yahoo.com> Compressed sections usually are the result of: 1- blunt knife 2- too soft paraffin for the selected thickness 3- too warm paraffin (block not cold enough) 4- blade too vertical (not enough clearing angle) 5- cutting too fast, specially with thin sections 6- too warm blade 7- microtome set screws loose or defective. Ren? J. --- On Wed, 3/11/09, Va Paula Sicurello wrote: From: Va Paula Sicurello Subject: [Histonet] Bad sections To: "HistoNet" Date: Wednesday, March 11, 2009, 2:29 PM Hi Histo netters, I am am my wit's end. I know how to section but my sections are compressing like crazy. I've altered my processing protocol thinking that I was overprocessing my samples. I've tried different knife angles, different brands of razor blade knives. It's either me or the microtome. Info- I process animal tissues with between 30-60 minute steps. Vacuum paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish degrees (a little below the middle line on the knife holder). Soak blocks on Downey ice blocks, water bath at 44 degrees. Suggestions? Waa, Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lwenk <@t> sbcglobal.net Wed Mar 11 23:40:24 2009 From: lwenk <@t> sbcglobal.net (Lee Wenk) Date: Wed Mar 11 23:40:46 2009 Subject: [Histonet] Bad sections In-Reply-To: <933464.47492.qm@web46113.mail.sp1.yahoo.com> References: <933464.47492.qm@web46113.mail.sp1.yahoo.com> Message-ID: <50F3B088D8A24B4EBA3FA93715437AFB@LeeWenkPC> Did you change paraffins lately? Softer paraffins, ie., lower melting point, have more compressibility. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Va Paula Sicurello" To: "HistoNet" Sent: Wednesday, March 11, 2009 11:29 AM Subject: [Histonet] Bad sections > > Hi Histo netters, > > I am am my wit's end. I know how to section but my sections are > compressing like crazy. I've altered my processing protocol thinking that > I was overprocessing my samples. > > I've tried different knife angles, different brands of razor blade knives. > > It's either me or the microtome. > > Info- I process animal tissues with between 30-60 minute steps. Vacuum > paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish > degrees (a little below the middle line on the knife holder). > Soak blocks on Downey ice blocks, water bath at 44 degrees. > > Suggestions? > > Waa, > > Paula > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBraaten <@t> Cheshire-Med.COM Thu Mar 12 04:58:35 2009 From: CBraaten <@t> Cheshire-Med.COM (Christine I. Braaten) Date: Thu Mar 12 04:58:49 2009 Subject: [Histonet] manual tissue processing Message-ID: Good morning everyone! Our Tissue Tek VIP 5 stopped working last night and I am waiting for someone at Sakura (Pacific time) to call me(Eastern time). Does anyone have a program for processing small biopsies manually? I searched the archives and was unable to find anything. I would appreciate any help I can get. Thanks . Christine Braaten The Cheshire Medical Center Keene, NH 03467 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From sbreckenridge <@t> caperegional.com Thu Mar 12 06:33:52 2009 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Thu Mar 12 06:33:59 2009 Subject: [Histonet] chilling paraffin blocks question Message-ID: <4D95C24EC0E4C84787A6919F1165B25E015B255A@btmhems01.BTHOSP.INT> Wondering what are your thoughts on chilling paraffin blocks via a tissue freezing spray (e.g. ones used to further chill frozen sections)? Thanks, Sue Breckenridge, Histology Supervisor BS, HT/HTL(ASCP) Cape Regional Medical Center Cape May Court House, NJ Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED. The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From sbreckenridge <@t> caperegional.com Thu Mar 12 06:41:27 2009 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Thu Mar 12 06:41:33 2009 Subject: [Histonet] chilling paraffin blocks question Message-ID: <4D95C24EC0E4C84787A6919F1165B25E015B255B@btmhems01.BTHOSP.INT> Wondering what are your thoughts on chilling paraffin blocks via a tissue freezing spray (e.g. ones used to further chill frozen sections)? Thanks, Sue Breckenridge, Histology Supervisor BS, HT/HTL(ASCP) Cape Regional Medical Center Cape May Court House, NJ Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED. The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From sbreckenridge <@t> caperegional.com Thu Mar 12 06:41:46 2009 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Thu Mar 12 06:41:54 2009 Subject: [Histonet] Recall: chilling paraffin blocks question Message-ID: <4D95C24EC0E4C84787A6919F1165B25E015B255C@btmhems01.BTHOSP.INT> Breckenridge, Sue would like to recall the message, "chilling paraffin blocks question". Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED. The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From rjbuesa <@t> yahoo.com Thu Mar 12 07:30:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 12 07:31:01 2009 Subject: [Histonet] chilling paraffin blocks question In-Reply-To: <4D95C24EC0E4C84787A6919F1165B25E015B255A@btmhems01.BTHOSP.INT> Message-ID: <254669.6170.qm@web65715.mail.ac4.yahoo.com> Some of my histotechs used to do that but just the instant before taking the section, after all the rimming was done. They had good results using the same spray used for doing FS. Ren? J. --- On Thu, 3/12/09, Breckenridge, Sue wrote: From: Breckenridge, Sue Subject: [Histonet] chilling paraffin blocks question To: histonet@pathology.swmed.edu Date: Thursday, March 12, 2009, 7:33 AM Wondering what are your thoughts on chilling paraffin blocks via a tissue freezing spray (e.g. ones used to further chill frozen sections)? Thanks, Sue Breckenridge, Histology Supervisor BS, HT/HTL(ASCP) Cape Regional Medical Center Cape May Court House, NJ Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED. The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara.Stancel <@t> fsis.usda.gov Thu Mar 12 08:12:02 2009 From: Barbara.Stancel <@t> fsis.usda.gov (Stancel, Barbara) Date: Thu Mar 12 08:12:11 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 17 In-Reply-To: <65A9121E3GW14291-01@tweed3east> References: <65A9121E3GW14291-01@tweed3east> Message-ID: Kris, If you want to melt paraffin for changing the processor, etc. you might think about getting a paraffin dispenser and a separate slide dryer. We have lab heat issues in the summer. We got rid of the incubator that melted our paraffin and got a Paraffin dispenser (TBS cat # PD-120). It holds 6.25 gal of paraffin at whatever temp we want. It takes about 3-6 hrs to completely melt or we turn it on the night before to use the next morn. It has a coffee pot type spigot for dispensing. It took us a while to get used to not having liquid paraffin at any time we want, but the benefit was an extra 2-3 degrees of cool air during our hot summer months. We have dedicated slide dryers. One is mobile and gets put on our downdraft during the summer heat. We even have the embedding centers with timers so we can eliminate some of their heat. Now if we can just figure out how to keep those two embedding centers from rising the lab temp while we are embedding................ Barbara Barbara Stancel, HTL(ASCP)HT USDA, FSIS, EL, Pathology RRC, 950 College Station Road Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stancel@fsis.usda.gov Message: 20 Date: Wed, 11 Mar 2009 09:40:18 -0400 From: "Kalleberg, Kristopher" Subject: incubator oven Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted before the processing of the slides. I will be using this oven for strictly melting paraffin containers to have ready for embedding center and for baking of slides to melt paraffin prior to processing. Has anyone had any concerns or issues with these new convection ovens or can they recommend a decent oven to buy. it just needs to be a medium sized oven. Thanks in advance. Kris kalleberg kristopher.kalleberg@unilever.com From eca9 <@t> georgetown.edu Thu Mar 12 08:22:18 2009 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Thu Mar 12 08:22:31 2009 Subject: [Histonet] silly question about goat serum Message-ID: <49B90C8A.9070604@georgetown.edu> Good morning, I have a question that is probably very silly but I just don't want to make any mistakes with the experiment I am suppose to do. I am used to the protein blocking being called normal goat serum (10%). The kit I am about to use says their's is 10% goat non-immune serum. Is this the same thing? Thanks for your help of a confused tech, Eva Permaul Georgetown University From rjbuesa <@t> yahoo.com Thu Mar 12 08:47:08 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 12 08:47:13 2009 Subject: [Histonet] silly question about goat serum In-Reply-To: <49B90C8A.9070604@georgetown.edu> Message-ID: <431184.22913.qm@web65712.mail.ac4.yahoo.com> Yest, it is. Ren? J. --- On Thu, 3/12/09, Eva Permaul wrote: From: Eva Permaul Subject: [Histonet] silly question about goat serum To: histonet@lists.utsouthwestern.edu Date: Thursday, March 12, 2009, 9:22 AM Good morning, I have a question that is probably very silly but I just don't want to make any mistakes with the experiment I am suppose to do. I am used to the protein blocking being called normal goat serum (10%). The kit I am about to use says their's is 10% goat non-immune serum. Is this the same thing? Thanks for your help of a confused tech, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Mar 12 09:14:55 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Mar 12 09:15:43 2009 Subject: [Histonet] Re: Bad sections Message-ID: Paula, Try changing your microtome blade. Get as many samples as you can from the companies and try them all out. We see a difference in sectioning paraffin blocks just by changing blades. You'll find something that works well for your paraffin and room ambient conditions. If your room is warm, that may also contribute to compression. Ice down (or spray) the knife edge and see if the colder blade also helps. Also, get rid of the Downy, you don't need it unless you need to soften some hard samples. Ice water should chill and rehydrate the blocks just fine. If you need more than that routinely, you are overprocessing your tissues. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From jlhowery <@t> yrmc.org Thu Mar 12 09:41:23 2009 From: jlhowery <@t> yrmc.org (jeff) Date: Thu Mar 12 09:41:34 2009 Subject: [Histonet] Dako Message-ID: <000601c9a320$9e216740$3394640a@yrmc.org> I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From talulahgosh <@t> gmail.com Thu Mar 12 09:41:30 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Mar 12 09:41:38 2009 Subject: [Histonet] silly question about goat serum In-Reply-To: <431184.22913.qm@web65712.mail.ac4.yahoo.com> References: <49B90C8A.9070604@georgetown.edu> <431184.22913.qm@web65712.mail.ac4.yahoo.com> Message-ID: What exactly does non-immune goat serum mean? The goat hasn't been exposed to any specific antigen, therefore it's normal? Emily -- prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves From emerald_lake77 <@t> yahoo.com Thu Mar 12 09:47:07 2009 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Mar 12 09:47:16 2009 Subject: [Histonet] Biotinylate antibodies kit Message-ID: <438864.8247.qm@web110605.mail.gq1.yahoo.com> Hello all, ? What kit(s) are people using nowadays to biotinylate their own antibodies?? I looked online and?saw numerous kits available.? I figured I'd ask my peers to see if their was a quick and easy kit out there. ? Any info, would be helpful. ? Thank you. ? Gustave ? ? Gustave T. Hebert Research Scientist I Wyeth Research Cambridge MA 02140 ? (Keyowrds: biotin, biotinylation, kit, antibody, antibodies, conjugate) From gvdobbin <@t> ihis.org Thu Mar 12 09:54:56 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Mar 12 09:55:13 2009 Subject: [Histonet] silly question about goat serum Message-ID: Hi Emily, Normal as opposed to immune serum. If a goat outside of a "Specific Pathogen Free (SPF)" facility were to actually have no Ab's it would be far from normal! I'm picturing the Boy in a Bubble episode on Seinfeld- only substitute one kid for another!! LOL (or groan if you prefer). Enjoy the day folks! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Emily Sours 3/12/2009 11:41:30 AM >>> What exactly does non-immune goat serum mean? The goat hasn't been exposed to any specific antigen, therefore it's normal? Emily -- prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From billodonnell <@t> catholichealth.net Thu Mar 12 09:58:30 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 09:58:46 2009 Subject: [Histonet] Dako In-Reply-To: <000601c9a320$9e216740$3394640a@yrmc.org> References: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: Haven't had it happen yet, but I have been systematically firing them for a number of reasons, not the least of which is no contact with any sales reps by their initialization for close to 8 YEARS! (Except when I complained about this and they showed up unexpectedly on my day off, so it doesn't count) Another reason is the new pricing on items, I am saving tons of money by shopping around. I am finding that revalidation with similar products hasn't involved too much change. In fact, most changes have been to oour benefit. Elimination/repackaging of detection systems, movement toward automation specific packaging(now in a small lab that has a small panel and can easily handle the work load manually) Gripe, Gripe Moan, Moan William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff Sent: Thursday, March 12, 2009 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Mar 12 10:02:05 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Mar 12 10:02:16 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: <000601c9a320$9e216740$3394640a@yrmc.org> References: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: <49B923ED.7060104@pathology.washington.edu> Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Mar 12 10:06:56 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 12 10:07:08 2009 Subject: [Histonet] Dako In-Reply-To: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: <968221.61812.qm@web65705.mail.ac4.yahoo.com> Ah the wonders of capitalism and the offer-demand paradigm! For?blackmail to take place, as to tango, it takes two. If they behave that way, get another provider. Perhaps if many just to that, they will start to behave as they used to be, friendly and accommodating. It most be the result of a new corporate "sales pushing wizard". Ren? J. --- On Thu, 3/12/09, jeff wrote: From: jeff Subject: [Histonet] Dako To: histonet@lists.utsouthwestern.edu Date: Thursday, March 12, 2009, 10:41 AM I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 12 10:07:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 12 10:08:05 2009 Subject: [Histonet] silly question about goat serum In-Reply-To: Message-ID: <591749.86244.qm@web65712.mail.ac4.yahoo.com> Just exactly that! Ren? J. --- On Thu, 3/12/09, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] silly question about goat serum To: histonet@lists.utsouthwestern.edu Date: Thursday, March 12, 2009, 10:41 AM What exactly does non-immune goat serum mean? The goat hasn't been exposed to any specific antigen, therefore it's normal? Emily -- prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Thu Mar 12 10:08:00 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu Mar 12 10:08:20 2009 Subject: [Histonet] Dako Message-ID: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu> No, but the price of their DAB+ did jump drastically. I like the product, but I may have to find an alternative. Tom Pier >>> "jeff" 03/12/09 9:47 AM >>> I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anne.lewin <@t> bms.com Thu Mar 12 10:13:53 2009 From: anne.lewin <@t> bms.com (Lewin, Anne) Date: Thu Mar 12 10:14:13 2009 Subject: [Histonet] Dako In-Reply-To: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu> References: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu> Message-ID: <4895A1696F956D4CB56011A8C61312820100505DC8@ushpwbmsmmp008.one.ads.bms.com> We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >to them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain >confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of the >original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Mar 12 10:19:49 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Mar 12 10:20:13 2009 Subject: [Histonet] Von Kossa staining on PMMA sections Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light. There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV. Does anyone know why such a long staining time is recommended? Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed? Thanks. From godsgalnow <@t> aol.com Thu Mar 12 10:23:33 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Mar 12 10:23:57 2009 Subject: [Histonet] Derm Cases Message-ID: <8CB713DA3EBA272-1158-7EB@webmail-db05.sysops.aol.com> Hey Everyone.... I am looking for a microwave processing protocol for large derm cases, such as excisional melanoma cases..... Any thoughts? Looking for process to include fixation, and fixative used Subsequent processing steps, to include solutions used, time and temperature And grossing tips....how think are they been grossed in etc.... Thanks in advance, Roxanne From pmarcum <@t> vet.upenn.edu Thu Mar 12 10:24:01 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 12 10:24:12 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: <49B923ED.7060104@pathology.washington.edu> References: <000601c9a320$9e216740$3394640a@yrmc.org> <49B923ED.7060104@pathology.washington.edu> Message-ID: <000401c9a326$929828e0$095a5b82@vet.upenn.edu> AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suhyoung.jeong <@t> gmail.com Thu Mar 12 10:28:05 2009 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Thu Mar 12 10:28:09 2009 Subject: [Histonet] anti-NFS1 antibody for staining? Message-ID: <450012a20903120828j153d9490hbfcd3b0f2af2456a@mail.gmail.com> Does anyone know any good antibody against human NFS1 (nitrogen-fixation 1 for bacteria, cysteine desulfurase for human) for cell staining? Thank you in advance Suh From Lynn.Burton <@t> Illinois.gov Thu Mar 12 10:46:09 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Mar 12 10:47:31 2009 Subject: [Histonet] Dako, Thermo and others References: <000601c9a320$9e216740$3394640a@yrmc.org><49B923ED.7060104@pathology.washington.edu> <000401c9a326$929828e0$095a5b82@vet.upenn.edu> Message-ID: Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Thu Mar 12 10:48:46 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Thu Mar 12 10:49:20 2009 Subject: [Histonet] RE: Von Kossa staining on PMMA sections In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <5B6165D78AC14544974A844787B47E3804C0374EBA@MAIL5.ad.uams.edu> Our Von Kossa Procedure calls for placing the slides in Silver Nitrate Solution for 2 minutes in the dark, rinse in distilled water, develop in Hydroquinone Solution for 1 minute, fix in Sodium Thiosulfate, Rinse, counterstain with Acid Fuchsin rinse in 1% Glacial Acetic Acid Solution quickly dehydrate, clear and mount. I will be happy to send you the procedure if you would like it. These sections are undecalcified bone embedded in MMA. We do this procedure with GMA also. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, March 12, 2009 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining on PMMA sections The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light. There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV. Does anyone know why such a long staining time is recommended? Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JWeems <@t> sjha.org Thu Mar 12 10:53:20 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 12 10:53:26 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: References: <000601c9a320$9e216740$3394640a@yrmc.org><49B923ED.7060104@pathology.washington.edu><000401c9a326$929828e0$095a5b82@vet.upenn.edu> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53AC5B7@ITSSSXM01V6.one.ads.che.org> Leica acquired Surgipath, not DAKO... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, March 12, 2009 11:46 AM To: Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From dellav <@t> musc.edu Thu Mar 12 10:53:47 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Mar 12 10:54:00 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: <49B923ED.7060104@pathology.washington.edu> References: <000601c9a320$9e216740$3394640a@yrmc.org> <49B923ED.7060104@pathology.washington.edu> Message-ID: I wasn't aware that Thermo now has the rights for the PSLIM. Thanks for posting this Victor. I did have an interest in this printer at one time, but based on this information I won't be considering it. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Mar 12 10:55:34 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Mar 12 10:55:57 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: References: <000601c9a320$9e216740$3394640a@yrmc.org><49B923ED.7060104@pathology.washington.edu> <000401c9a326$929828e0$095a5b82@vet.upenn.edu> Message-ID: <49B8F835.2B7F.00C9.0@geisinger.edu> I'm pretty sure Danaher owns Surgipath now.....along with nearly everything else..... Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Burton, Lynn" 3/12/2009 11:46 AM >>> Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From gmartin <@t> marshallmedical.org Thu Mar 12 11:05:50 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Mar 12 11:05:55 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA53AC5B7@ITSSSXM01V6.one.ads.che.org> References: <000601c9a320$9e216740$3394640a@yrmc.org><49B923ED.7060104@pathology.washington.edu><000401c9a326$929828e0$095a5b82@vet.upenn.edu> <5D64396A0D4A5346BEBC759022AAEAA53AC5B7@ITSSSXM01V6.one.ads.che.org> Message-ID: <6ED9D4252F278841A0593D3D788AF24C04C2EB50@mailsvr.MARSHMED.local> That's correct ... Leica = Surgipath -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, March 12, 2009 8:53 AM To: Burton, Lynn; Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Leica acquired Surgipath, not DAKO... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, March 12, 2009 11:46 AM To: Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Mar 12 11:06:08 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Mar 12 11:06:14 2009 Subject: [Histonet] Surgipath Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635E31@hpes1.HealthPartners.int> Leica bought Surgipath, not Dako!!!!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From SwainFrancesL <@t> uams.edu Thu Mar 12 11:09:48 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Thu Mar 12 11:12:11 2009 Subject: [Histonet] RE: Von Kossa staining on PMMA sections In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <5B6165D78AC14544974A844787B47E3804C0374EC9@MAIL5.ad.uams.edu> I believe that that is because of the thickness of the sections and the fact that they are in plastic. Also if the plastic is GMA you cannot remove it and so therefore it takes longer for it to penetrate. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, March 12, 2009 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining on PMMA sections The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light. There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV. Does anyone know why such a long staining time is recommended? Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From contact <@t> excaliburpathology.com Thu Mar 12 11:14:18 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Mar 12 11:14:26 2009 Subject: [Histonet] Dako Message-ID: <927177.95432.qm@web1104.biz.mail.sk1.yahoo.com> I believe it was about a year ago that the family that started Dako finally sold it.?The "new powers that be"?have since raised prices 3 to 4 times. Luckily I do not need very many of their products and am now trying to avoid them, as well as other companies that have gobbled?up smaller companies.? I also don't buy from companies that try to make even more by charging outragious shipping and handling. I had one company try to charge $65 for shipping one tiny vial of antibody. I cancelled the order and found it from another company. I own my own lab and write out the checks and pay the bills, so I see what everything costs. I have found that the Vector ImmPress detection works just as well as Envision, if not better, with less background and?Vector charges actual shipping. I have no invested interest in Vector, just a happy customer. With the brunt of the bad economy yet to hit, these companies?need to?remember?business needs customers. PKP From HornHV <@t> archildrens.org Thu Mar 12 11:17:55 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Mar 12 11:18:19 2009 Subject: [Histonet] Dako In-Reply-To: <4895A1696F956D4CB56011A8C61312820100505DC8@ushpwbmsmmp008.one.ads.bms.com> References: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu> <4895A1696F956D4CB56011A8C61312820100505DC8@ushpwbmsmmp008.one.ads.bms.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830A9@EMAIL.archildrens.org> My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >to them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain >confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of the >original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From akbitting <@t> geisinger.edu Thu Mar 12 11:31:46 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Mar 12 11:32:05 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA53AC5B7@ITSSSXM01V6.one.ads.che.org> References: <000601c9a320$9e216740$3394640a@yrmc.org><49B923ED.7060104@pathology.washington.edu><000401c9a326$929828e0$095a5b82@vet.upenn.edu> <5D64396A0D4A5346BEBC759022AAEAA53AC5B7@ITSSSXM01V6.one.ads.che.org> Message-ID: <49B900B1.2B7F.00C9.0@geisinger.edu> Danaher owns them both. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Weems, Joyce" 3/12/2009 11:53 AM >>> Leica acquired Surgipath, not DAKO... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, March 12, 2009 11:46 AM To: Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From godsgalnow <@t> aol.com Thu Mar 12 11:34:10 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Mar 12 11:34:26 2009 Subject: [Histonet] Dako In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830A9@EMAIL.archildrens.org> References: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu><4895A1696F956D4CB56011A8C61312820100505DC8@ushpwbmsmmp008.one.ads.bms.com> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830A9@EMAIL.archildrens.org> Message-ID: <8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com> All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain >confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of the >original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Mar 12 11:46:36 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Mar 12 11:46:56 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: Message-ID: Not Dako - Leica purchased Surgipath. "Burton, Lynn" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/12/2009 10:46 AM To "Pamela Marcum" , "Victor Tobias" , "jeff" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Dako, Thermo and others Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu Mar 12 11:59:18 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Mar 12 11:59:35 2009 Subject: [Histonet] Dako, Thermo and others In-Reply-To: References: Message-ID: LOL! It's ALMOST Friday, people, ALMOST... :-) --On Thursday, March 12, 2009 11:46 AM -0500 Jackie M O'Connor wrote: > Not Dako - Leica purchased Surgipath. > > > > > > "Burton, Lynn" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/12/2009 10:46 AM > > To > "Pamela Marcum" , "Victor Tobias" > , "jeff" > cc > histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Dako, Thermo and others > > > > > > > Dako did acquire Surgipath recently. The Surgipath rep said it has been a > good "marriage" for them so far. > > Lynn Burton > Lab Assoc. I > Animal Disease Lab > Galesburg, Il 61401 > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum > Sent: Thu 3/12/2009 10:24 AM > To: 'Victor Tobias'; 'jeff' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Dako, Thermo and others > > > > AMEN!!!! > > Pamela A Marcum > University of Pennsylvania > School of Veterinary Medicine > Comparative Orthopedic Laboratory (CORL) > 382 W Street Rd > Kennett Square PA 19438 > 610-925-6278 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor > Tobias > Sent: Thursday, March 12, 2009 11:02 AM > To: jeff > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Dako, Thermo and others > > Jeff, > > It is not just Dako, but many vendors have jacked up the cost of goods. > Let's take the PSLIM from Accuplace. About a month ago you could > purchase this for $5K. Now that Thermo has acquired distribution rights > it costs over $10K and nothing has changed. To me, it is better to sell > 10 at $5K than 5 at $10K. We were ready to purchase several more of > these, but have to reconsider our options now. No wonder Healthcare > costs are out of control. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > jeff wrote: >> I was wondering if anyone else is being blackmailed by Dako to sign a > contract with them. We have enjoyed our relationship a number of years > buying there product as we need it. We also have changed detection kits as > we where advised. Now they are telling us that if we do not go with price > per slide they will raise our costs of purchasing our supplies. Example > EnVision + dual link system last year cost 1,580.70 this year 3,300 and > that > this will go in effect March 1st ( just talked to the rep about this Tues) > Just 1 example. SOOOO. Has anyone else had this happen to them and what > did > you do? Thanks Jeff >> >> Confidentiality Notice: This e-mail message, including any attachments, > is >> for the sole use of the intended recipient(s) and may contain > confidential >> and privileged information. Any unauthorized review, use, disclosure or >> distribution is prohibited. If you are not the intended recipient, > please >> contact the sender by reply e-mail and destroy all copies of the > original >> message. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From pkromund <@t> gundluth.org Thu Mar 12 12:35:24 2009 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Thu Mar 12 12:35:31 2009 Subject: [Histonet] Re: Histonet Digest, Vol 64, Issue 15 In-Reply-To: Message-ID: HI Laurie, Have you tried cutting only one section per slide & fixing it immediately, allowing it no time to air dry. This is our practice we place only one section per slide & place the slide immediately in fixative. The pathologist have become extremely particular about this step. You may be getting air-dried artifact. It's worth giving it a try. Pamela Romundstad Gundersen Lutheran 1910 South Ave No. LaCrosse, WI 54601 608-775-3139 From MLafrini <@t> csmlab.com Thu Mar 12 12:36:37 2009 From: MLafrini <@t> csmlab.com (Michael LaFriniere) Date: Thu Mar 12 12:37:15 2009 Subject: [Histonet] Dako In-Reply-To: <000601c9a320$9e216740$3394640a@yrmc.org> References: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: <49B90FD1.588C.00AF.0@csmlab.com> Someone has to pay for their yearly world wide sales meetings...Dako just had theirs in South Africa...imagine what that cost! >>> On 3/12/2009 at 10:41 AM, "jeff" wrote: I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Mar 12 12:42:43 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Mar 12 12:43:02 2009 Subject: [Histonet] Dako References: <8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com> Message-ID: <62A8156F8071C8439080D626DF8C33A65D8BA6@wave-mail.7thwave.local> Innovex is a wonderful company that offers good products at an extremely reasonable cost and provides exceptional tech support. No, I don't work for them...I've just had great experiences. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain >confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of the >original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From billodonnell <@t> catholichealth.net Thu Mar 12 12:52:54 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 12:53:15 2009 Subject: [Histonet] Dako In-Reply-To: <8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com> References: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu><4895A1696F956D4CB56011A8C61312820100505DC8@ushpwbmsmmp008.one.ads.bms.com><9AE8AA9E1F644B4AA6C155FB6FD51C6317D830A9@EMAIL.archildrens.org> <8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com> Message-ID: That's who I went with as well. At first I wrote them off because of all the star trek references, but products are not notch and customer service is great. Prices are still in the easonable" range, though, as a result of the recession I might expect a price jump. Even if their detection kits doouble, they will still be better priced that Dako. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Mar 12 12:53:42 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Mar 12 12:53:52 2009 Subject: [Histonet] Tissue processors Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238A7@PHSXMB30.partners.org> To all: Sorry to bother the list with a question that has no doubt been addressed in the past, but now that we are looking into possibly replacing our tissue processor, I would like some input. It no longer is covered by a service contract and things are starting to go wrong with it. We have a Hypercenter XL (ThermoShandon). We are a core pathology lab for a research group. Typically we run @ 1000-1500 paraffin blocks/year, so we are looking at a processor that would meet our needs. We have limited space, so something, size-wise, like the Hypercenter XL would be required. What do most people prefer? If you would like to contact me off-list, my email is below. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From billodonnell <@t> catholichealth.net Thu Mar 12 12:58:00 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 12:58:10 2009 Subject: [Histonet] Dako In-Reply-To: <49B90FD1.588C.00AF.0@csmlab.com> References: <000601c9a320$9e216740$3394640a@yrmc.org> <49B90FD1.588C.00AF.0@csmlab.com> Message-ID: Well, to be fair, worldwide sales conferences have to be somewhere. 'Tis true it 'tis almost Friday, and a wee bit longer til' St. Patty's William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Thursday, March 12, 2009 12:37 PM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] Dako Someone has to pay for their yearly world wide sales meetings...Dako just had theirs in South Africa...imagine what that cost! >>> On 3/12/2009 at 10:41 AM, "jeff" wrote: I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu Mar 12 13:04:35 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Mar 12 13:04:58 2009 Subject: [Histonet] Dako In-Reply-To: References: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu><4895A1696F956D4CB56011A8C61312820100505DC8@ushpwbmsmmp008.one.ads.bms.com><9AE8AA9E1F644B4AA6C155FB6FD51C6317D830A9@EMAIL.archildrens.org><8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com> Message-ID: <8CB715422B8EC33-64C-E8B@Webmail-mg09.sim.aol.com> Plus, they have Joe Meyer!? He can come and give workshops at your facility for which you can get CEUs for, as he has been approved by NSH as a provider. -----Original Message----- From: O'Donnell, Bill To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 1:52 pm Subject: RE: [Histonet] Dako That's who I went with as well. At first I wrote them off because of all the star trek references, but products are not notch and customer service is great. Prices are still in the easonable" range, though, as a result of the recession I might expect a price jump. Even if their detection kits doouble, they will still be better priced that Dako. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM T o: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Thu Mar 12 13:21:19 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu Mar 12 13:22:06 2009 Subject: [Histonet] Dako Message-ID: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> I've had great luck with Biocare as well. They've been my primary supplier for detection kits and ancillaries since 2003. I'm probably going to check them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL. Tom Pier >>> "O'Donnell, Bill" 03/12/09 12:56 PM >>> That's who I went with as well. At first I wrote them off because of all the star trek references, but products are not notch and customer service is great. Prices are still in the easonable" range, though, as a result of the recession I might expect a price jump. Even if their detection kits doouble, they will still be better priced that Dako. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu Mar 12 13:28:10 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Mar 12 13:28:40 2009 Subject: [Histonet] Dako In-Reply-To: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> References: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> Message-ID: <8CB71576E307995-64C-1002@Webmail-mg09.sim.aol.com> My favorite is Betazoid DAB -----Original Message----- From: Thomas Pier To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; billodonnell@catholichealth.net; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 2:21 pm Subject: RE: [Histonet] Dako I've had great luck with Biocare as well. They've been my primary supplier for detection kits and ancillaries since 2003. I'm probably going to check them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL. Tom Pier >>> "O'Donnell, Bill" 03/12/09 12:56 PM >>> That's who I went with as well. At first I wrote them off because of all the star trek references, but products are not notch and customer service is great. Prices are still in the easonable" range, though, as a result of the recession I might expect a price jump. Even if their detection kits doouble, they will still be better priced that Dako. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way S lot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwes tern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Mar 12 13:30:01 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Mar 12 13:30:14 2009 Subject: [Histonet] Bone specimens Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635E37@hpes1.HealthPartners.int> Does your facility require bone from a total hip or knee replacements from patients with osteoarthritis or Degenerative Joint Disease to be sent to pathology, or are these samples exempt? If they are sent to pathology, do they perform a gross only or gross+micro. Thank You, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From marktarango <@t> gmail.com Thu Mar 12 13:36:22 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Mar 12 13:36:25 2009 Subject: [Histonet] Dako In-Reply-To: <000601c9a320$9e216740$3394640a@yrmc.org> References: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: <5b6eb13e0903121136k4479b4b2s3699f2d19bdc1377@mail.gmail.com> We've been told that we can't get any pricing except for list price unless we sign a contract. Our purchasing department asked me to start shopping around for replacement antibodies. In fact, I'm looking for a replacement for their SMA antibody. Looks like I'm going with AbSerotec for this antibody. I've started to order from other vendors such as BioCare too. Dako just doesn't get it. Mark On Thu, Mar 12, 2009 at 7:41 AM, jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a > contract with them. We have enjoyed our relationship a number of years > buying there product as we need it. We also have changed detection kits as > we where advised. Now they are telling us that if we do not go with price > per slide they will raise our costs of purchasing our supplies. Example > EnVision + dual link system last year cost 1,580.70 this year 3,300 and > that this will go in effect March 1st ( just talked to the rep about this > Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and > what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dermpathsy <@t> gmail.com Thu Mar 12 13:38:13 2009 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Thu Mar 12 13:38:25 2009 Subject: [Histonet] Factor XIIIa in epidermal cell nuclei Message-ID: <8854ff80903121138l249be58bl8a1d26fefb49e9e@mail.gmail.com> Greetings everyone .. I think this may have been mentioned on this list once before in 2005 (judging from the Archives), but I would appreciate your input. Do other people use clone AC-1A1 of Factor XIIIa? .. That's the antibody we use here and I am not particularly comfortable with this antibody staining the nuclei of epidermal cells. Was anybody able to fix this with that clone, or do we just have to look for another source? .. We went down to a 1:800 dilution and the nuclear staining is still there. At 1:1000, the staining is very weak both in dermal dendritic cells as well as in the epidermis .. Thank you Sate -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From ratliffjack <@t> hotmail.com Thu Mar 12 13:39:59 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 12 13:40:13 2009 Subject: [Histonet] Von Kossa staining on PMMA sections In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CA5@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: Paul, For MMA embedded specimens (MMA +DBP), I first deplastify my sections, hydrate to water, stain in 5% silver nitrate solution for 5 minutes (in the dark), wash times three changes in DI H2O (in the dark), develop in sodium-carbonate formaldehyde solution for 2 minutes (in the dark), wash times two in DI H2O (back under normal lighting conditions), then stop the reaction in sodium thiosulfate + potassium ferricyanide solution for 30 seconds, and immediately rinse in running tap water for 15 minutes. The Von Kossa reaction results from process above then yields black mineralized bone. After the tap water rinse, I generally counterstain with 2% MacNeal's tetrachrome for 5 minutes, rinse in DI H2O, and dehydrate to xylenes to coverslip. This then reveals immature bone formation or osteoid = grayish or jaded green, growth plate cartilage = purple, osteoblasts = blue, osteoclasts = blue-green, bloods cells = greenish, etc. Feel free to contact me if you have any additional questions. Jack > Date: Thu, 12 Mar 2009 11:19:49 -0400 > From: PMonfils@Lifespan.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Von Kossa staining on PMMA sections > > The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light. There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV. Does anyone know why such a long staining time is recommended? Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed? Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Mar 12 13:53:06 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Mar 12 13:53:09 2009 Subject: [Histonet] Dako In-Reply-To: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: Dako has lost their way. Customer service and affordability has suffered badly since the buyout. Luckily, there are always other vendors...and this is coming from a Dako fanatic. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From anh2006 <@t> med.cornell.edu Thu Mar 12 13:51:52 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Mar 12 13:55:12 2009 Subject: [Histonet] Dako In-Reply-To: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu> References: <49B8DF00020000DF00016170@gwmail.medicine.wisc.edu> Message-ID: <1270209968-1236884126-cardhu_decombobulator_blackberry.rim.net-1209132254-@bxe1028.bisx.prod.on.blackberry> What does their DAB+ cost now?? I love it and find it to be most reliable but find Zymed's DAB+ to be just as good (I think Zymed may be a part of Invitrogen now?). -----Original Message----- From: Thomas Pier Date: Thu, 12 Mar 2009 10:08:00 To: ; Subject: Re: [Histonet] Dako No, but the price of their DAB+ did jump drastically. I like the product, but I may have to find an alternative. Tom Pier >>> "jeff" 03/12/09 9:47 AM >>> I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Mar 12 13:59:12 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 12 13:59:17 2009 Subject: [Histonet] Dako In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8BA6@wave-mail.7thwave.local> References: <8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com> <62A8156F8071C8439080D626DF8C33A65D8BA6@wave-mail.7thwave.local> Message-ID: <002801c9a344$a205ea10$095a5b82@vet.upenn.edu> DIDO They have been great with me and I do some really strange animal stuff. Innovex has always helped or if they did know said so, which I really appreciate. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, March 12, 2009 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Innovex is a wonderful company that offers good products at an extremely reasonable cost and provides exceptional tech support. No, I don't work for them...I've just had great experiences. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain >confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of the >original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stevenhacker <@t> verizon.net Thu Mar 12 13:59:56 2009 From: stevenhacker <@t> verizon.net (Steven Hacker) Date: Thu Mar 12 14:00:12 2009 Subject: [Histonet] Dako Message-ID: <11397402.417268.1236884396520.JavaMail.root@vms062.mailsrvcs.net> Hi, First, I DO work for BioCare Medical on the ea Godsgalnow, you are right. Joe Myers is a great guy, very hel and knowledgable. I'm not an HT, but I sure have learned a LOT from hi Bill, as far as the Star Trek references, our Director of Scie Affairs-David Tacha-is a Star Trek fanatic from WAY back. If you eve r get to go into his office, there's a replica scanner and phaser plus two bookcase. He's a multiplex staining system an BioCare is a good company to work for and with. I've worked fo several laboratory companies-in my 21 years of lab sales-and I've only go as BioCare Regards, Steven Hacker Mar 12, 2009 02:07:39 PM, [1]god Plus, they ha your facility for which yo approved by NSH as a provider. -----Original Message----- From: O'Donnell, Bill <[2]billodonnell@catholichealth.net> To: [3]godsg [5]anne.lewin@bms.com; [6]histonet@lists.utsout [7]jlhowery@yrmc.org Sent: Thu, 12 Mar 20 Subject: RE: [Histonet] Dako That's who I went of all the star trek re service is great. Pric though, as a result of the recessi their detection kits doouble, th Dako. William (Bill) O'Donnell, HT ( Lead Histologist Good Samaritan Hospital 10 East 31st Kearney, NE 68847 -----Original Message----- From: [mailto:[9]histonet-bounces@lis Of [10]godsgalnow@aol.com< Sent: Thursday, March 12, 2009 11:34 AM To: [11]Horn [13]histonet@lists.utsouthwestern.edu; [14]jlhower Subject: Re: [Histonet] Dako All of you fed up wit out along time ago and switch now I have been using them for touch.? They still do reagent ren discounts based on purchasing.? Hey... -----Original Message----- From: H To: Lew [17]histonet@lists.utsouthwestern.edu; [18]jlhowery@yrmc. Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako< My purchasing agent notified me of the huge markup of their< BR>reagents/antibodies. I called my rep told them I was unhappy and wouldhave to look elsewhere and they decided to give me the old pricing for a< Hazel Horn Hazel Horn, Supervisor of Histology Arkansas Children's Hospital1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4 fax 501.364.3155 visit us on the web at: [19]www.archildrens.org -----Original Message----- From: [20]histonet-bounces@lists.utsouthwestern.edu [mailto:[21]histonet-bounces@lists.utsouthwestern.edu] On Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM T o: [22]histonet@lists.utsouthwestern.edu; [23]jlhowery@yrmc.org Subject: RE: [Histonet] Dako We n is taking some sort of having to do more Anne >-----Original Message----- >From: [24]histonet-bounces@lists.utsouthwestern.edu [mailt o:histonet- >[25]bounces@lists.utsouthwestern >Sent: Thursday, March 12, 2009 11 >To: [26]histonet@lists.utsouthwestern >Subject: Re: [Histonet] > >No, but the price of their DAB+ did jump drastically. I >product, but I may have to find an alternative. >>Tom Pier > >>>> "jeff" <[28]jlhowery@ >I was wondering if an sign a >contract with them years >buying there p detection kits >as we go with > supplies. & this year >3,300 and that this will go in effect March 1st ( just talked to the< >about this Tues) Just 1 example. SOOOO. Has anyone else had t happen >t o them and what did you do? Thanks Jeff >< attachmen >is for the sole use of the intended recipient(s) and may con >confidential and privileged information. Any unauthorized revi ew, use, >disclosure or distribution is prohibited. If you are not t intended >recipient, please contact the sender by reply e-mail an all >copies of the original message. >______________ >Histonet mailing list >[29]Histonet@lists.utsouthwestern.edu >[30]http://lists.utsouthwestern.edu/mailman/listinfo/his > > >__________________________________________ >Histonet mailing list >[31]H >[32]h _______ Histonet mailing list [33]Histonet@lists.utsouthwestern.edu [34]http://lists.utsouthwestern.edu/mailman/listinfo/histonet *************************************************************** ********* ************************************************************** ********** ************************************************************* *********** ************************************************************ ************ *********************************************************** ************* ********************************************************** ************** ********************************************************* *************** ******************************************************** **************** ******************************************************< information contained in this message may be privileged and confi message is responsible for deli recipient, you are hereby notified t copying of this communication is this communication in error, p to the m essage and deleting it Thank you. __________________________________ Histonet mailing list [35]Histo [36]http://li _______________ Histonet mailing list [37]Histonet@lists.utsouthwestern.edu [38]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing lis [39]Histonet@lists.utsouthwestern.edu < href="http://lists.utsouthwestern.edu/mailman/listin fo/histonet" target=_blank>http://lists.utsouthwestern.edu/mailman/listin fo/histonet References 1. 3D"mailto:godsgalnow@aol.com" 2. 3D"mailto:billodonnell@catholichealth.net" 3. 3D"mailto:godsgalnow@aol.com" 4. 3D"mailto:Horn 5. 3D"mailto:anne.lewin@bms.com" 6. file://localhost/tmp/3D"mail 7. 3D"mailto:jlhow 8. 3D"mailto:histonet-bounces@lists 9. 3D"mailto:hist 10. 3D"mailto:godsgalnow@aol.com" 11. 3D"mailto:HornHV@archildrens.org" 12. file://localhost/tmp/3D"mailto 13. 3D"mailto:histonet@lists.utsouthwestern.edu" 14. 3D"mailto:jlhowery@yrmc.org" 15. 3D"mailto:HornHV 16. 3D"mailto:anne.lewin 17. 3D"mailto:histonet@lists.utsouthwestern.edu" 18. 3D"mailto:jlhowery@yrmc.org" 19. 3D"http://www.archildrens.org"/ 20. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 21. 3D"mailto:histonet-bounces@lists.utsouthwest 22. 3D"mailto:histonet@lists.utso 23. 3D"mailto:jlhowery@yrmc.org" 24. 3D"mailto:histonet-bounces@lists.utsouthwester 25. 3D"mailto:bou 26. 3D"mailto:histo 27. 3D"mailto:jlhowery@yrmc 28. 3D"mailto:jlhowery@yrmc.org" 29. 3D"mailto:Histonet@lists.utsouthwester 30. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his 31. 3D"mailto:Histonet@lists.utsouthwestern.edu" 32. file://localhost/tmp/3D"h 33. 3D"mailto:Histonet@lists.utsouthwestern 34. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 35. 3D"mailto:Histonet@lists.utsouthwestern.edu" 36. 3D"http://li=/ 37. 3D"mailto:Histonet@lists.utsouthwestern.edu" 38. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 39. 3D"mailto:Histonet@lists.uts From anh2006 <@t> med.cornell.edu Thu Mar 12 13:58:40 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Mar 12 14:01:21 2009 Subject: [Histonet] Dako In-Reply-To: References: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: <1088727408-1236884493-cardhu_decombobulator_blackberry.rim.net-133209903-@bxe1028.bisx.prod.on.blackberry> DAKO reps used to be great, it is a pity. We have not been contacted in mnay years either. I used to learn so much from them back when I was newer to the field. -----Original Message----- From: "O'Donnell, Bill" Date: Thu, 12 Mar 2009 08:58:30 To: jeff; Subject: RE: [Histonet] Dako Haven't had it happen yet, but I have been systematically firing them for a number of reasons, not the least of which is no contact with any sales reps by their initialization for close to 8 YEARS! (Except when I complained about this and they showed up unexpectedly on my day off, so it doesn't count) Another reason is the new pricing on items, I am saving tons of money by shopping around. I am finding that revalidation with similar products hasn't involved too much change. In fact, most changes have been to oour benefit. Elimination/repackaging of detection systems, movement toward automation specific packaging(now in a small lab that has a small panel and can easily handle the work load manually) Gripe, Gripe Moan, Moan William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff Sent: Thursday, March 12, 2009 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Mar 12 14:01:18 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Mar 12 14:02:36 2009 Subject: [Histonet] Dako, Thermo and others References: <000601c9a320$9e216740$3394640a@yrmc.org><49B923ED.7060104@pathology.washington.edu><000401c9a326$929828e0$095a5b82@vet.upenn.edu> <5D64396A0D4A5346BEBC759022AAEAA53AC5B7@ITSSSXM01V6.one.ads.che.org> Message-ID: Thanks for the heads up! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Thu 3/12/2009 10:53 AM To: Burton, Lynn; Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Leica acquired Surgipath, not DAKO... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, March 12, 2009 11:46 AM To: Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Dako did acquire Surgipath recently. The Surgipath rep said it has been a good "marriage" for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN!!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: > I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Mar 12 14:06:06 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Mar 12 14:06:13 2009 Subject: [Histonet] RE:DAKO In-Reply-To: <20090312155633.AE5146253D2@barracuda.sdstate.edu> References: <20090312155633.AE5146253D2@barracuda.sdstate.edu> Message-ID: I don't understand why your prices are so different from mine. I just got a new quote from my Rep and my price increases were very reasonable. There seems to be a large discrepancy in prices across the USA. I would call the office at DAKO and complain or like you said change vendors. I would also ask what percentage of the increase goes to the Rep. and what role the Rep has in the price increases. We have an excellent Rep. Margaret Perry From pmarcum <@t> vet.upenn.edu Thu Mar 12 14:12:11 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 12 14:12:16 2009 Subject: [Histonet] Dako In-Reply-To: <002801c9a344$a205ea10$095a5b82@vet.upenn.edu> References: <8CB714781919B72-64C-873@Webmail-mg09.sim.aol.com><62A8156F8071C8439080D626DF8C33A65D8BA6@wave-mail.7thwave.local> <002801c9a344$a205ea10$095a5b82@vet.upenn.edu> Message-ID: <003b01c9a346$725c9c80$095a5b82@vet.upenn.edu> Correction If they did not know the answer they told me and I really appreciate that. 'Cause some companies and people just keep talking when they don't know and it does not help. Just wastes time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 12, 2009 2:59 PM To: 'Michele Wich'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako DIDO They have been great with me and I do some really strange animal stuff. Innovex has always helped or if they did know said so, which I really appreciate. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, March 12, 2009 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Innovex is a wonderful company that offers good products at an extremely reasonable cost and provides exceptional tech support. No, I don't work for them...I've just had great experiences. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain >confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of the >original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Mar 12 14:22:55 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Mar 12 14:23:00 2009 Subject: [Histonet] Dako References: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> Message-ID: Why do I keep feeling more and more like these guys are the 'contractors', and we are the 'government'. i.e. $50 screwdrivers come to mind... I think we need to start looking into these guys like others are looking into the pharmaceutical market pricing. They think they have us over a barrel. I say we put them in the barrel and pitch them over the falls. (the higher the better) How about paying for their goods based on how much we get reimbursed by insurance/midicare/etc for the testing using their products. Although I have to say my little experience with immunos (on derm frozen sections, no less) I was really happy with Biocare. They sent tech specialists to help out with protocols and troubleshooting even though we didn't even know if we were even going to do the testing at all. Customer service was always great and very patient. They even transfered me to a specialist if they didn't know the answers! Even their kits were compatable with other antibodies, and I was using predilutes. Never failed me, and improved the staining of the other brand antibodies when their own kits didn't work. Sorry. Tons of stress and migranes the past few days. I might be on the verge of going Histo if I'm not careful. Is it Friday yet? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Pier Sent: Thu 3/12/2009 1:21 PM To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; billodonnell@catholichealth.net; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako I've had great luck with Biocare as well. They've been my primary supplier for detection kits and ancillaries since 2003. I'm probably going to check them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL. From Doug.Showers <@t> propath.com Thu Mar 12 14:35:03 2009 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Thu Mar 12 14:36:28 2009 Subject: [Histonet] Dako In-Reply-To: References: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> Message-ID: <82C7248978CB50469FD6BA68EBBEFE67EB4748@exchange.propathlab.com> Claire, One of the things that irritates me about some companies is their tendency to charge for their products based solely on what we get reimbursed rather than being happy with making a healthy profit based on their expenses. Doug Showers, MS, HT Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, March 12, 2009 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Why do I keep feeling more and more like these guys are the 'contractors', and we are the 'government'. i.e. $50 screwdrivers come to mind... I think we need to start looking into these guys like others are looking into the pharmaceutical market pricing. They think they have us over a barrel. I say we put them in the barrel and pitch them over the falls. (the higher the better) How about paying for their goods based on how much we get reimbursed by insurance/midicare/etc for the testing using their products. Although I have to say my little experience with immunos (on derm frozen sections, no less) I was really happy with Biocare. They sent tech specialists to help out with protocols and troubleshooting even though we didn't even know if we were even going to do the testing at all. Customer service was always great and very patient. They even transfered me to a specialist if they didn't know the answers! Even their kits were compatable with other antibodies, and I was using predilutes. Never failed me, and improved the staining of the other brand antibodies when their own kits didn't work. Sorry. Tons of stress and migranes the past few days. I might be on the verge of going Histo if I'm not careful. Is it Friday yet? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Pier Sent: Thu 3/12/2009 1:21 PM To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; billodonnell@catholichealth.net; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako I've had great luck with Biocare as well. They've been my primary supplier for detection kits and ancillaries since 2003. I'm probably going to check them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Thu Mar 12 14:37:55 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 14:38:07 2009 Subject: [Histonet] Dako In-Reply-To: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> References: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> Message-ID: I just wanted to thank everyone for not buzzing me about all my typos in the post! Man, that was bad! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: Thomas Pier [mailto:tp2@medicine.wisc.edu] Sent: Thursday, March 12, 2009 1:21 PM To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; O'Donnell, Bill; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako I've had great luck with Biocare as well. They've been my primary supplier for detection kits and ancillaries since 2003. I'm probably going to check them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL. Tom Pier >>> "O'Donnell, Bill" 03/12/09 12:56 >>> PM >>> That's who I went with as well. At first I wrote them off because of all the star trek references, but products are not notch and customer service is great. Prices are still in the easonable" range, though, as a result of the recession I might expect a price jump. Even if their detection kits doouble, they will still be better priced that Dako. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies. I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year. I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them. Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically. I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go with >price per slide they will raise our costs of purchasing our supplies. >Example EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do? Thanks Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Thu Mar 12 14:50:33 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 14:50:43 2009 Subject: [Histonet] Dako In-Reply-To: <1088727408-1236884493-cardhu_decombobulator_blackberry.rim.net-133209903-@bxe1028.bisx.prod.on.blackberry> References: <000601c9a320$9e216740$3394640a@yrmc.org> <1088727408-1236884493-cardhu_decombobulator_blackberry.rim.net-133209903-@bxe1028.bisx.prod.on.blackberry> Message-ID: Actually, one of their greatest assets in the early days was their customer service and the information they were able to offer on such a new technology. I had many students (OJT)practically that gray immunoperopxidase book (can't recall the name)they used to hand out. Five years ago, when we went to ao formalin-free microwave processor, I contacted them and asked if they would be willing to help me work out the validations for IHC and was told they weren't interested. Turns out, of course that I didn't need them anyway, but still, we were buying a whole lotta stuff from them. Started firing them after that response. So, do I have a bitter taste left behind - yes, but also some fond memories of the "good old days" when they were the best game in town. Doubt they coould win my business back. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Thursday, March 12, 2009 1:59 PM To: O'Donnell, Bill; histonet-bounces@lists.utsouthwestern.edu; jeff; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako DAKO reps used to be great, it is a pity. We have not been contacted in mnay years either. I used to learn so much from them back when I was newer to the field. -----Original Message----- From: "O'Donnell, Bill" Date: Thu, 12 Mar 2009 08:58:30 To: jeff; Subject: RE: [Histonet] Dako Haven't had it happen yet, but I have been systematically firing them for a number of reasons, not the least of which is no contact with any sales reps by their initialization for close to 8 YEARS! (Except when I complained about this and they showed up unexpectedly on my day off, so it doesn't count) Another reason is the new pricing on items, I am saving tons of money by shopping around. I am finding that revalidation with similar products hasn't involved too much change. In fact, most changes have been to oour benefit. Elimination/repackaging of detection systems, movement toward automation specific packaging(now in a small lab that has a small panel and can easily handle the work load manually) Gripe, Gripe Moan, Moan William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff Sent: Thursday, March 12, 2009 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Thu Mar 12 14:58:59 2009 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Mar 12 14:59:06 2009 Subject: [Histonet] Dako In-Reply-To: References: <000601c9a320$9e216740$3394640a@yrmc.org> Message-ID: I would like to ask how any of you feel about the Dako's Flex system. I'm having a hard time liking a system that is supposed to be a strong polymer, but needs a higher pH retrieval step to work. I also find myself tittering antibodies to a lesser degree. I should explain I have two jobs: one research (full time), and one hospital. But because they are such different settings, I see things that make me scratch my head about materials and pricing. In research I am also having problems with ordering at a reasonable price. I realize this is the same for only some of you. Envision+ was my detection kit of choice. However, my price limit is $2500 per order, and I find myself unable to order one detection kit for the autostainer I purchased. Even for ancillary items, I have to split the orders. Like Glen, I was a staunch Dako supporter. I was the first one to place their stainer in my state. I like and respect their people, and they have never failed me in the past. I just can't afford them. Hugh-Hawaii > Date: Thu, 12 Mar 2009 13:53:06 -0500 > From: GDawson@dynacaremilwaukee.com > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Dako > > Dako has lost their way. Customer service and affordability has suffered badly since the buyout. Luckily, there are always other vendors...and this is coming from a Dako fanatic. > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From billodonnell <@t> catholichealth.net Thu Mar 12 15:05:50 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 15:06:03 2009 Subject: [Histonet] Dako In-Reply-To: References: <000601c9a320$9e216740$3394640a@yrmc.org><1088727408-1236884493-cardhu_decombobulator_blackberry.rim.net-133209903-@bxe1028.bisx.prod.on.blackberry> Message-ID: OK that's IT! I'm going to start using my spellchecker! :) Friday seems early this week, at least on this forum! Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, March 12, 2009 2:51 PM To: anh2006@med.cornell.edu; histonet-bounces@lists.utsouthwestern.edu; jeff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Actually, one of their greatest assets in the early days was their customer service and the information they were able to offer on such a new technology. I had many students (OJT)practically that gray immunoperopxidase book (can't recall the name)they used to hand out. Five years ago, when we went to ao formalin-free microwave processor, I contacted them and asked if they would be willing to help me work out the validations for IHC and was told they weren't interested. Turns out, of course that I didn't need them anyway, but still, we were buying a whole lotta stuff from them. Started firing them after that response. So, do I have a bitter taste left behind - yes, but also some fond memories of the "good old days" when they were the best game in town. Doubt they coould win my business back. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Thursday, March 12, 2009 1:59 PM To: O'Donnell, Bill; histonet-bounces@lists.utsouthwestern.edu; jeff; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako DAKO reps used to be great, it is a pity. We have not been contacted in mnay years either. I used to learn so much from them back when I was newer to the field. -----Original Message----- From: "O'Donnell, Bill" Date: Thu, 12 Mar 2009 08:58:30 To: jeff; Subject: RE: [Histonet] Dako Haven't had it happen yet, but I have been systematically firing them for a number of reasons, not the least of which is no contact with any sales reps by their initialization for close to 8 YEARS! (Except when I complained about this and they showed up unexpectedly on my day off, so it doesn't count) Another reason is the new pricing on items, I am saving tons of money by shopping around. I am finding that revalidation with similar products hasn't involved too much change. In fact, most changes have been to oour benefit. Elimination/repackaging of detection systems, movement toward automation specific packaging(now in a small lab that has a small panel and can easily handle the work load manually) Gripe, Gripe Moan, Moan William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff Sent: Thursday, March 12, 2009 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. SOOOO. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Thu Mar 12 15:19:38 2009 From: Dolores_Fischer <@t> baxter.com (Dolores_Fischer@baxter.com) Date: Thu Mar 12 15:19:43 2009 Subject: [Histonet] Dako Message-ID: My colleague and I both love BioCare products (and personnel). She also has recently gotten the Intellipath immunotainer and loves it. I also like Vector products and their tech support. Just my two cents!! D The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From tjasper <@t> copc.net Thu Mar 12 16:42:00 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Mar 12 16:42:05 2009 Subject: [Histonet] Dako Message-ID: <90354A475B420441B2A0396E5008D4965E301B@copc-sbs.COPC.local> OK, There've been plenty of shots taken at Dako...and from what I can tell rightly so. I agree with the statements about the "good old days" and all, and I would still like to believe Dako is a decent antibody company. So here's my question - How 'bout it Dako? Are you going to take all of this lying down? Do you have any response(s) at all? You (collectively as a company) probably owe a lot of these folks some sort of explanation. I find it hard to believe that Dako has opted to adopt a business model, which (from all appearances) has taken a path of self-destruction. And I don't care who the parent company is now (Danaher?) these anecdotes exemplify irresponsible handling of business. It certainly isn't necessary to have attended Harvard (insert your favorite) business school to draw this conclusion. I also believe that competition is good for the marketplace. By having Dako, a one-time leader in the field, basically "tank" isn't good for anyone. I'd like to see something salvaged here, but it's not up to me. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 541/693-2677 tjasper@copc.net From CIngles <@t> uwhealth.org Thu Mar 12 16:58:29 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Mar 12 16:59:27 2009 Subject: [Histonet] Dako References: <90354A475B420441B2A0396E5008D4965E301B@copc-sbs.COPC.local> Message-ID: Can anyone say AIG/Freddie Mac/Fannie Mae? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Thu 3/12/2009 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako I also believe that competition is good for the marketplace. By having Dako, a one-time leader in the field, basically "tank" isn't good for anyone. I'd like to see something salvaged here, but it's not up to me. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 541/693-2677 tjasper@copc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sarah_tarran <@t> wmi.usyd.edu.au Thu Mar 12 15:53:42 2009 From: sarah_tarran <@t> wmi.usyd.edu.au (Sarah Tarran) Date: Thu Mar 12 17:04:57 2009 Subject: [Histonet] Re: IHC chromogen loss, HELP! In-Reply-To: <20090312132200.8E0751A6D7E@seive.med.usyd.edu.au> References: <20090312132200.8E0751A6D7E@seive.med.usyd.edu.au> Message-ID: <60099.192.195.170.5.1236891222.squirrel@www.wmi.usyd.edu.au> Hi Meghan, We had the same problem with Vector VIP - I stain both atherosclerotic specimens and burns - for some reason the athero samples were fine but the burns compeltely lost their colour. It turned out, for us, it was the alcohol in the dehydration step that was stripping the colour. I would check the slides after they had been stained and the staining was fine but once I looked at them after coverslipping there was no chromagen. I now airdry before taking the slides to histoclear. So my protocol is (from chromogen onwards): vector VIP - 15 mins H20 rinse for 5 mins Haematoxylin H20 rinse 5 minutes airdry (usually overnight but just until slides are completely dry) histoclear x 2, 5 mins each vectormount Hope this helps. I am not sure with the chromogens you are using but i know you cant use Xylene with vector blue so this might be the case with the other ones as well Good luck > Message: 10 > Date: Wed, 11 Mar 2009 15:04:07 -0400 > From: "Woodward, Denise" > Subject: [Histonet] IHC chromogen loss, HELP! > To: > Message-ID: > <40AC6D73C2B95C4CA21B26B7BF380C4002D0E4FF@EXCHANGED.mgmt.ad.uconn.edu> > Content-Type: text/plain; charset="us-ascii" > > Posting for a friend.......... > > > > "We have hit a bump in the road with our IHC test and thought maybe you > might have some input as we seem to be stuck. We stain (typically for > virus) frozen bovine tissues using Biocare's Mach-3 AP polymer kit and > develop with Vector Red, counterstain, and dehydrate and mount with > Histoclear/VectaMount by hand or xylene/Surgipath mountant on the > autocoverslipper. We have been noticing a major loss of stain after > development recently. The controls seem to remain stained, it's the more > diffuse and minimally stained sections that we have seen stain disappear > between development and coverslipping. I did a test with Impress-DAB and > Impress-Bajoran Purple to compare and we thought we had solved the > problem as being related to the surgipath mountant. However I just did a > run with cell markers coverslipping with the Histoclear/Vectamount and > there seems to be a loss of stain again. Vector suggested trying 200 mM > TBS for wash/diluent/stop instead of 100 mM. This is a recent problem as > we have been using this protocol successfully for a few years now. Any > suggestions would be greatly appreciated!" > > > > Replies directly to Meghan.tucker@ARS.USDA.GOV would be appreciated > > > > > Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital, Westmead, NSW, 2145 02 98455775 sarah_tarran@wmi.usyd.edu.au From akemiat3377 <@t> yahoo.com Thu Mar 12 17:48:42 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Mar 12 17:48:47 2009 Subject: [Histonet] Dako 2008 Beckman Coulter and Milestones Message-ID: <694254.64164.qm@web31304.mail.mud.yahoo.com> Hi All, Dako was not purchased by Danaher. FYI.. Read posted Milestones from Dako's website below: Regards, Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com Corporate Milestones 2008 Beckman Coulter buys Dako?s Flow Business Unit Dako?s Flow Business Unit in Colorado, USA has been sold to Beckman Coulter. Thereby Dako?s focus on anatomic pathology, tissue-based cancer diagnostics and reagents for flow cytometry is strengthened. 2007 Dako launches Dako Link - Integrated Workflow Solutions Never before has Dako launched so many important products at one time. The Dako Link solution offers software, instruments, reagents and connectivity options customized to meet the needs of any pathology laboratory. Dako Link substantially improves cost efficiency and turnaround time in the lab by linking together the entire workflow. Dako gets new owner Private equity fund EQT V (?EQT?) has signed a definitive agreement to acquire 100% of Dako. The total consideration for the transaction is DKK 7.25 billion. 2006 Dako launches ACIS? III Dako launches the newest addition to our growing portfolio of automated platforms, ACIS? III. With a new sophistication, ACIS? IIII is enhancing the lab?s workflow to include advanced image analysis benefits in a simple manner. Pathologists now have the capability to improve their workflow, turnaround time, and experience cost savings. Dako's Microbiology business sold The sale of the microbiology activities to Oxoid is part of Dako?s strengthened focus on anatomic pathology. Dako?s microbiology range will gradually be rebranded as Oxoid and products should be ordered via Oxoid. 2005 Change of name The company changes its name from DakoCytomation A/S to Dako A/S. Microbiology up for sale DakoCytomation sharpens its focus on cancer diagnostics by putting its Microbiology activities up for sale. Agreement on image analysis DakoCytomation enters an agreement with Clarient Inc. on the further development and sale of its ACIS instruments, which are used for image analysis. The agreement is part of Dako?s strategy to automate more areas of the workflow in the pathology laboratory and to offer more integrated systems to the market for cancer diagnostics. 2002 Acquires instrument business DakoCytomation acquires the Artisan? instrument business from CytoLogix Inc. Artisan? is a state-of-the-art tissue staining instrument. Merges with bioinstrumentation company Cytomation and becomes DakoCytomation DAKO, the world-leading producer of cancer diagnostic reagents, merges with the Fort Collins-based bioinstrumentation company Cytomation Inc. The purpose of the merger is for the two companies to utilize their common competencies to further strengthen their position in the fast-growing market segments of the in vitro diagnostics industry. Cytomation was originally founded in 1988 in Colorado by one American and three Australian scientists who were convinced that the industry-standard flow cytometer could do a better job. 2001 Sells Boston Probes DAKO sells Boston Probes Inc. to Applied Biosystems Inc. 1996 Expands into PNA technology DAKO co-founds Boston Probes Inc. with the purpose of expanding into PNA technology, a supplementary technology to the traditional DNA technology. 1992 Danish pharmaceutical company Novo Nordisk A/S invests in DAKO 1991 Acquires Novo Nordisk Diagnostics DAKO acquires Novo Nordisk Diagnostics (activities in microbiology) in Cambridge, United Kingdom. 1990 DAKOPATTS changes name to DAKO 1980 Establishes subsidiaries In the 1980s DAKOPATTS establishes more sales subsidiaries around the world. Today the company has a total of 22 subsidiaries. 1979 Enters the USA DAKOPATTS Corporation is founded in the USA. 1966 Founded in Denmark DAKOPATTS is founded in Denmark. DAKOPATTS? initial product series was purified polyclonal antibodies. Press Releases From tameyers <@t> roadrunner.com Thu Mar 12 18:23:25 2009 From: tameyers <@t> roadrunner.com (tameyers@roadrunner.com) Date: Thu Mar 12 18:23:29 2009 Subject: [Histonet] Please remove me from any further mailings Message-ID: <20090312232325.7L74X.282907.root@hrndva-web18-z01> No offense, I just would rather use the website to seek info, because I do not have the time to keep up with all of the emails. Thank you, Trish Meyers From akemiat3377 <@t> yahoo.com Thu Mar 12 18:35:06 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Mar 12 18:35:09 2009 Subject: [Histonet] Dako gets new owner Message-ID: <128202.3015.qm@web31307.mail.mud.yahoo.com> Hi All, Is it Friday yet? I have been ill and my synaptic responses are miss-firing. Beckman Coulter bought Dako's Flow business unit and in 2007 the Private equity fund EQT V (?EQT?) signed a definitive agreement to acquire 100% of Dako. The total consideration for the transaction is DKK 7.25 billion. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From Susan.Walzer <@t> HCAHealthcare.com Fri Mar 13 02:17:46 2009 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Mar 13 02:18:03 2009 Subject: [Histonet] Tissue processors In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238A7@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238A7@PHSXMB30.partners.org> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2AABE0691B@FWDCWPMSGCMS09.hca.corpad.net> I have not heard much good about the Shandon processor. For reliability go with a VIP every time. This comes from MANY years in the field. I have never had problems with one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, March 12, 2009 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processors To all: Sorry to bother the list with a question that has no doubt been addressed in the past, but now that we are looking into possibly replacing our tissue processor, I would like some input. It no longer is covered by a service contract and things are starting to go wrong with it. We have a Hypercenter XL (ThermoShandon). We are a core pathology lab for a research group. Typically we run @ 1000-1500 paraffin blocks/year, so we are looking at a processor that would meet our needs. We have limited space, so something, size-wise, like the Hypercenter XL would be required. What do most people prefer? If you would like to contact me off-list, my email is below. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Mar 13 02:53:14 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Mar 13 02:53:27 2009 Subject: [Histonet] 20 micron resin sections Message-ID: Hi all, I have a query from a colleague doing research on neuroanatomy as to whether it is possible ( with relative ease) to cut 20mu sections from JB4 resin embedded tissue? Apparently these sections ae to be stained and then used for stereomicroscopy. My experience is not that extensive to be able to answer her, so I would appreciate some advice here best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Laura.Miller <@t> leica-microsystems.com Fri Mar 13 05:02:05 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Fri Mar 13 05:02:34 2009 Subject: [Histonet] Laura Miller is out of the office. Message-ID: I will be out of the office starting 03/13/2009 and will not return until 03/23/2009. I am on vacation. Since I will be out of the country, I will not be checking emails. I will respond to your email when I return on March 23, 2009. Thanks! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From pmarcum <@t> vet.upenn.edu Fri Mar 13 05:54:36 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Mar 13 05:54:43 2009 Subject: [Histonet] Tissue processors In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2AABE0691B@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <2017871265.60386331236941676377.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> I would disagree as I am on my third Shandon in a long career and have never had a problem with any of them. Age and growth in nneds got them. Currently we use a Pathcentre and it has been excellent. If you haven't used one it is hard to comment on how they work and how the company works with you. I have used VIPs and they are good. It is preference and comfort on purchasing a unit. Pam Marcum ----- Original Message ----- From: "Walzer Susan" To: "Margaret Sherwood" , histonet@lists.utsouthwestern.edu Sent: Friday, March 13, 2009 3:17:46 AM GMT -05:00 US/Canada Eastern Subject: RE: Re: [Histonet] Tissue processors I have not heard much good about the Shandon processor. For reliability go with a VIP every time. This comes from MANY years in the field. I have never had problems with one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, March 12, 2009 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processors To all: Sorry to bother the list with a question that has no doubt been addressed in the past, but now that we are looking into possibly replacing our tissue processor, I would like some input. It no longer is covered by a service contract and things are starting to go wrong with it. We have a Hypercenter XL (ThermoShandon). We are a core pathology lab for a research group. Typically we run @ 1000-1500 paraffin blocks/year, so we are looking at a processor that would meet our needs. We have limited space, so something, size-wise, like the Hypercenter XL would be required. What do most people prefer? If you would like to contact me off-list, my email is below. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Fri Mar 13 09:11:29 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Fri Mar 13 09:11:38 2009 Subject: [Histonet] Disposal of Formaldehyde Message-ID: <926491.92795.qm@web82001.mail.mud.yahoo.com> ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) From Bryan.Watson <@t> parkview.com Fri Mar 13 09:25:39 2009 From: Bryan.Watson <@t> parkview.com (Bryan Watson) Date: Fri Mar 13 09:25:58 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <926491.92795.qm@web82001.mail.mud.yahoo.com> References: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: <49BA34A4.5674.0085.1@parkview.com> I'm in Indiana. Here, it is up to the city. We dump all of ours down the drain and supposedly the city has ok'd that. They say that our hospital is big enough that all of the water used here will dilute the formalin enough to make it ok. However I had heard, back when I was in school that formalin kills the beneficial bacteria they use at water treatment plants. So I'm opposed to putting it down the drain. . . yet, that's how we do it here. Bryan >>> Jessica Piche 3/13/2009 10:11 AM >>> Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Fri Mar 13 09:26:35 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Fri Mar 13 09:26:44 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <926491.92795.qm@web82001.mail.mud.yahoo.com> References: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C04C2F537@mailsvr.MARSHMED.local> First ... even if the local authorities allow this ... it doesn't make "Green" sense to do it, especially when there are other very workable alternatives. The path we chose was to purchase a very simple gravity feed recycler produced and sold by Creative Waste Solutions (888) 795-8300. Check them out. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 7:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Mar 13 10:00:33 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Mar 13 10:00:41 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <926491.92795.qm@web82001.mail.mud.yahoo.com> References: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238B1@PHSXMB30.partners.org> We are discouraged from putting most any chemical down the drain. The MWRA (Massachusetts Water Resources Authority) monitors mercury levels, etc. in Boston Harbor (as well as waterways throughout the state) and will issue steep fines if labs are found to be dumping such waste down the drain. Consequently, our in-house Safety Office and our outside waste management company (Triumverate Environmental) monitor our waste. Alcohols, stains, and fixatives are disposed of as hazardous waste. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 10:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Janet.Bonner <@t> FLHOSP.ORG Fri Mar 13 10:06:53 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Mar 13 10:09:31 2009 Subject: [Histonet] Disposal of Formaldehyde References: <926491.92795.qm@web82001.mail.mud.yahoo.com> <6ED9D4252F278841A0593D3D788AF24C04C2F537@mailsvr.MARSHMED.local> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F295C@fhosxchmb006.ADVENTISTCORP.NET> The same recycler is used here for us. we are absolutely not allowed to put formaldehyde/formalin or EtOH or Xylene down the sink. If you don't use a recycler, hire a waste company to handle it. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Martin, Gary Sent: Fri 3/13/2009 10:26 AM To: Jessica Piche; histonet Subject: RE: [Histonet] Disposal of Formaldehyde First ... even if the local authorities allow this ... it doesn't make "Green" sense to do it, especially when there are other very workable alternatives. The path we chose was to purchase a very simple gravity feed recycler produced and sold by Creative Waste Solutions (888) 795-8300. Check them out. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 7:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From tpodawiltz <@t> lrgh.org Fri Mar 13 10:20:33 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Mar 13 10:20:43 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238B1@PHSXMB30.partners.org> References: <926491.92795.qm@web82001.mail.mud.yahoo.com>, <073AE2BEA1C2BA4A8837AB6C4B943D9703E238B1@PHSXMB30.partners.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BD12@LRGHEXVS1.practice.lrgh.org> We dump very little down the drain. Formalin has not been drained dump since 1988. We used to have it hauled away, because the former administration never got into re-cycling the formalin. What we do today, is too de-formalize the formalin, test it and then drain dumped if it passes. We buy the de-formalizer from Surgipath. We had to get this approved the State and we have to keep our records for 5 years. I keep the original and send a copy to the Director of Hazard Waste. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret [MSHERWOOD@PARTNERS.ORG] Sent: Friday, March 13, 2009 11:00 AM To: Jessica Piche; histonet Subject: RE: [Histonet] Disposal of Formaldehyde We are discouraged from putting most any chemical down the drain. The MWRA (Massachusetts Water Resources Authority) monitors mercury levels, etc. in Boston Harbor (as well as waterways throughout the state) and will issue steep fines if labs are found to be dumping such waste down the drain. Consequently, our in-house Safety Office and our outside waste management company (Triumverate Environmental) monitor our waste. Alcohols, stains, and fixatives are disposed of as hazardous waste. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 10:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From BMolinari <@t> heart.thi.tmc.edu Fri Mar 13 10:27:54 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 13 10:28:03 2009 Subject: [Histonet] dumping formalin Message-ID: We collect ours and the Hazardous Waste Dept of the hospital collects it. Now what they do with it I have no idea. We collect all our waste, fixatives, stains etc. I know of at least 2 facilities in Houston that pour theirs down the drain. I am from Boston I was so pleased to read the steps they have taken. They city spent a lot of money cleaning up the harbor. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From LINDA.MARGRAF <@t> childrens.com Fri Mar 13 10:31:22 2009 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri Mar 13 10:31:42 2009 Subject: [Histonet] DI water Message-ID: <49BA35FB.F783.00DA.0@childrens.com> Here's a message from Jennifer....(please respond to the list or her directly.... thanks) Hi everyone, We are getting ready to start running immunos at our lab and we need to install a DI water system. After talking to a friend who recently put in a system at his lab, I realize that there is a lot more to it than just simply putting a hole in the wall and tapping in some DI water. Anways, we are going to start running them by hand but, eventually want to be able to use an automatic stainer. Since the buffers require the use of DI water and it is recommended to it in the pressure cooker or decloaker for heat retrieval, we definitely need to put in a system. We will most likely be starting out running just a handful of stains but, would like to be able to increase that number eventually. Can anyone tell me what type of water you use (ultrapure, Cap type 2 etc), how much water (gpm), how much space in your lab it requires, do you need a certain pressure, and any other helpful tips? I appreciate it. Thank you. Jen Campbell Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
disclosures are prohibited without proper authorization. If you are not the intended recipient, any
disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
applicable privileges related to this information.

From tifei <@t> foxmail.com Fri Mar 13 11:05:26 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Fri Mar 13 11:05:38 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gRGlzcG9zYWwgb2YgRm9ybWFsZGVoeWRl?= References: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: <200903140005206905437@foxmail.com> Hard to say... we perfuse sooooooooooo many animals everyday...several litres for one lab into the sea 2009-03-14 TF ???? Jessica Piche ????? 2009-03-13 23:25:02 ???? histonet ??? ??? [Histonet] Disposal of Formaldehyde ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Fri Mar 13 11:20:29 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 13 11:20:36 2009 Subject: [Histonet] dumping formalin In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238B4@PHSXMB30.partners.org> Message-ID: Hi Margaret, You are right you can take the girl from Boston but not Boston out of the girl. Not to be picky...Bostonian...or has it been changed :0 My entire family is there and I go visit every summer. -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Friday, March 13, 2009 9:37 AM To: Molinari, Betsy Subject: RE: [Histonet] dumping formalin Betsy, Nice to hear from a (former)Bostonite. Although, if you are from Boston, you are always from Boston! You are right: the city did spend a lot of money cleaning up the harbor and all that feeds into it. MGH is right on the Charles River and I'm sure I don't have to tell you how disgusting that was! When I first moved here in 1968 (oops, dating myself!), I went sailing with friends on the Charles (Community Boating). We capsized and it's a wonder we all did not come down with typhoid! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, March 13, 2009 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dumping formalin We collect ours and the Hazardous Waste Dept of the hospital collects it. Now what they do with it I have no idea. We collect all our waste, fixatives, stains etc. I know of at least 2 facilities in Houston that pour theirs down the drain. I am from Boston I was so pleased to read the steps they have taken. They city spent a lot of money cleaning up the harbor. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Fri Mar 13 11:27:10 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 13 11:27:13 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: <422898.8047.qm@web65703.mail.ac4.yahoo.com> Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Mar 13 11:59:29 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Mar 13 11:59:36 2009 Subject: [Histonet] blades Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635E48@hpes1.HealthPartners.int> Kind of a "Friday" type of question inasmuch as it could open some "cutting" remarks.......how do others handle microtome blades that are not totally used? Our situation is that we use the more expensive "teflon coated" blades for certain tissues that are more delicate or difficult to obtain the most optimal sections and use the ceramic coated blades for routine microtomy. Obviously, the techs are oftentimes going to have a blade with an area that is still very usable and the dilemma is a safety issue as to where to place/store blades that are not ready to discard! Any good ideas??? Appreciate any "kind" ideas!! Thanks..... ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From carrolpb <@t> umdnj.edu Fri Mar 13 12:04:00 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Mar 13 12:04:07 2009 Subject: [Histonet] blades In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635E48@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA2705635E48@hpes1.HealthPartners.int> Message-ID: <49BA9200.8050409@umdnj.edu> i "store" them in the sharps waste container ;) Webb, Dorothy L wrote: > Kind of a "Friday" type of question inasmuch as it could open some > "cutting" remarks.......how do others handle microtome blades that are > not totally used? Our situation is that we use the more expensive > "teflon coated" blades for certain tissues that are more delicate or > difficult to obtain the most optimal sections and use the ceramic coated > blades for routine microtomy. Obviously, the techs are oftentimes going > to have a blade with an area that is still very usable and the dilemma > is a safety issue as to where to place/store blades that are not ready > to discard! Any good ideas??? Appreciate any "kind" ideas!! > Thanks..... > ________________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From akemiat3377 <@t> yahoo.com Fri Mar 13 12:14:51 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Mar 13 12:14:55 2009 Subject: [Histonet] Buy-outs Who owns Who Message-ID: <919281.39120.qm@web31301.mail.mud.yahoo.com> Happy Friday the 13th Histonet! Forewarning, this is a little long. There seems to be a lot of discussion as of late, to all the buy outs, and who owns who. I have been following this trend for several years with great interest. It is amazing how pricing, customer support and service, varies from one company to another, even though they are owned by the same parent company. I can certainly understand and relate to the frustration and anger of the end-user. There is so much disconnection within the companies! We need a score card to keep up with all the changes! I maybe wrong, but I don't think the sales reps even know the ownership tree. I put a tree at the end of this correspondence that I think is correct. I recently had a conservation with a HR rep from Thermo/Fisher, and she didn't even know who Peter Scheu was. Pretty telling of the knowledge out there. HR should at least know who is at the top of the chain! Quote: Peter Scheu has been Group President, Clinical Diagnostics of Apogent Technologies Inc. since September 2000. Mr. Scheu served as the President of Richard-Allan Scientific Company (?Richard-Allan?) from 1997 to 2000 and as Executive Vice President of Richard-Allan from 1995 to 1997. Let's dispell the confusion and please correct me if I am wrong. The ownership trees: Parent Company: Apogent Technologies Inc Thermo/Fisher Richard Allan Microm Lab Vision Neomarkers Antibodies Parent Company: Danaher Leica Microsystems Vision Biosystems (Bond) Novacastra NEW-Suripath NEW-McCormick Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From leiker <@t> buffalo.edu Fri Mar 13 12:16:24 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Mar 13 12:16:31 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <200903140005206905437@foxmail.com> References: <926491.92795.qm@web82001.mail.mud.yahoo.com> <200903140005206905437@foxmail.com> Message-ID: <2D09DAB0861E5DB9362A6B23@bchwxp2702.ad.med.buffalo.edu> Ugh. We are not allowed to dispose any chemical, including formaldehyde, down our drains here at UB (Buffalo, NY). Everything goes into appropriately-labeled waste bottles for Hazardous Waste to dispose of, however they do it. --On Saturday, March 14, 2009 12:05 AM +0800 TF wrote: > Hard to say... > we perfuse sooooooooooo many animals everyday...several litres for one lab > into the sea > > > 2009-03-14 > > > > TF > > > > ???? Jessica Piche > ????? 2009-03-13 23:25:02 > ???? histonet > ??? > ??? [Histonet] Disposal of Formaldehyde > > ? > Hi All, > We have a question regarding the disposal of formaldehyde. We were told > at our hospital that a consultant said it was okay to dump formaldehyde > down the drain. I believe they said it was okay to dump 15 gallons or so > a day! We are not to fond of this idea and would like to know what > everyone else is doing. How is everyone disposing of their formaldehyde? > We would be especially interested in what other hospitals in CT are > doing. Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From dgaupp <@t> tulane.edu Fri Mar 13 12:22:06 2009 From: dgaupp <@t> tulane.edu (Gaupp, Dina D ) Date: Fri Mar 13 12:22:10 2009 Subject: [Histonet] frozen section - spleen problems Message-ID: <447056A67472B241A330A525B4AF7167018042F6@EX02.ad.tulane.edu> Histoland: I am having problems sectioning spleen without artifact(holes) as well as pencil shaves. A little bit of background.... mouse spleen fresh frozen embedded in OCT (plastic mold) at -80C freezer. The tissue was then given to me already frozen in OCT mold. Each specimen consisted of 2 spleens and 2 hearts in one mold. The hearts cut fine, but the spleens cut like pencil shaves. The border of the spleen stayed intact, but the entire center would either section with huge gaps in the tissue or it shredded into pieces. Now the PI(Principle Investigator) wants to know what did I do wrong. I've tried warming the tissue before sectioning it with my finger as well as increasing the cryostat temp to -18C. Any warmer & the heart starts to malfunction upon sectioning. I told the PI not to embed 2-different samples in one block. I don't know what else to tell him or what else I can do to give him a better sample. I've tried telling him - its a frozen section of tissue - morphology will not be well preserved, you have higher chances of artifact than paraffin processed tissue. Again, remind you this tissue was given to me already frozen in OCT. I wish there was a way we could soften the tissue other than rubbing my finger across it. Anyone have any tricks up their sleeves to cut spleens perfectly?????? Do you think initially when freezing the spleen down, freeze it at a warmer temp. (-40C or -50C) & the tissue will become less brittle upon sectioning? I've never frozen tissue warmer than -76C. I know that freezing tissue any warmer than -80C can cause artifact & obstruct cellular/cytoplasmic morphology..... I've been doing a little background reading on this matter & I've read that some freeze their tissue at -40C or warmer & are able to get a section without artifact......why this can't be meeee. Histology seems to be a field of trial & error... what works for you might not work for someone else. I am glad we have histonet, because we can gather everyones do's & don'ts & come to our on conclusions. Thanking you all in advance for your advice.... Dina Dina D. Gaupp, BS, MT Histology Core Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu From NMP <@t> stowers.org Fri Mar 13 12:23:57 2009 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Fri Mar 13 12:24:34 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <422898.8047.qm@web65703.mail.ac4.yahoo.com> Message-ID: I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Mar 13 12:27:51 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 13 12:28:01 2009 Subject: [Histonet] blades In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635E48@hpes1.HealthPartners.int> Message-ID: I will save a blade in a plastic 5-slide mailer. I usually use it for trimming so that I don't waste a new blade. I label the container with "sharps" info. Jennifer "Webb, Dorothy L" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/13/2009 10:03 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] blades Kind of a "Friday" type of question inasmuch as it could open some "cutting" remarks.......how do others handle microtome blades that are not totally used? Our situation is that we use the more expensive "teflon coated" blades for certain tissues that are more delicate or difficult to obtain the most optimal sections and use the ceramic coated blades for routine microtomy. Obviously, the techs are oftentimes going to have a blade with an area that is still very usable and the dilemma is a safety issue as to where to place/store blades that are not ready to discard! Any good ideas??? Appreciate any "kind" ideas!! Thanks..... ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Fri Mar 13 12:30:27 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Fri Mar 13 12:32:37 2009 Subject: [Histonet] Disposal of Formaldehyde Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F6F9561@GIEXCHANGE.gidocs.net> I disagree also, it is up to the local wastewater treatment plant to decide if you may dispose of formalin down the drain, they also dictate how much. It is not prohibited across the board.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Friday, March 13, 2009 12:24 PM To: 'rjbuesa@yahoo.com'; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Fri Mar 13 12:35:02 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Mar 13 12:35:07 2009 Subject: [Histonet] RE: Old formalin and disposable blades In-Reply-To: <7304671700005520@HolyRedeemer.com> Message-ID: I've worked where it was acceptable by the city sewage authority to dump formalin down the drain. I've also worked where it was collected by a company as hazardous waste. Here, we collect and neutralize before drain disposal, a much more sensible method. As for dispo blades that are not quite "used up", I once had a tech ask me what the cost would be to treat a tech with a cut, compared with the cost of a blade. One of those things that make you go hmmmmmmmmm.....Since then, we don't leave blades on an unattended microtome. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From tjasper <@t> copc.net Fri Mar 13 12:40:26 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Mar 13 12:40:32 2009 Subject: [Histonet] Disposal of Formaldehyde References: Message-ID: <90354A475B420441B2A0396E5008D4965E301C@copc-sbs.COPC.local> You are correct Nanette. Waste water treatment is under the jurisdiction of local regulations from place to place. What's allowed in one place may not be in another. Seems it depends on what various waste treatment facilities have the ability to handle. It also seems, historically what has happened to water in a certain regions and what folks will tolerate. I'm not trying to excuse pollution, I'm just stating what I understand exists. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Friday, March 13, 2009 10:24 AM To: 'rjbuesa@yahoo.com'; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Fri Mar 13 12:44:43 2009 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Fri Mar 13 12:44:59 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <07732CE52EC3174AB891DE1C62DB4D8F6F9561@GIEXCHANGE.gidocs.net> References: <07732CE52EC3174AB891DE1C62DB4D8F6F9561@GIEXCHANGE.gidocs.net> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60801F41@msxc06.OSUMC.EDU> I agree. It is up to each state/county/city/municipality or whatever to determine what their regulations are, and facilities must comply with the most stringent set of rules that they are governed by. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Friday, March 13, 2009 1:30 PM To: Marsh, Nannette; rjbuesa@yahoo.com; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I disagree also, it is up to the local wastewater treatment plant to decide if you may dispose of formalin down the drain, they also dictate how much. It is not prohibited across the board.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Friday, March 13, 2009 12:24 PM To: 'rjbuesa@yahoo.com'; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Fri Mar 13 12:46:22 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Fri Mar 13 12:46:26 2009 Subject: [Histonet] Dako Message-ID: <614833.52798.qm@web58601.mail.re3.yahoo.com> I?highly suggest Biocare. It is nice to use a company that still cares about their customers. I love their products/equipment. They have great customer service, sales reps. and their service/tech help?is top notch. I made the switch?years ago too. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 3/12/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: RE: [Histonet] Dako To: "Thomas Pier" , godsgalnow@aol.com, HornHV@archildrens.org, anne.lewin@bms.com, histonet@lists.utsouthwestern.edu, jlhowery@yrmc.org Date: Thursday, March 12, 2009, 3:37 PM I just wanted to thank everyone for not buzzing me about all my typos in the post! Man, that was bad! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: Thomas Pier [mailto:tp2@medicine.wisc.edu] Sent: Thursday, March 12, 2009 1:21 PM To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; O'Donnell, Bill; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako I've had great luck with Biocare as well.? They've been my primary supplier for detection kits and ancillaries since 2003.? I'm probably going to check them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL. Tom Pier >>> "O'Donnell, Bill" 03/12/09 12:56 >>> PM >>> That's who I went with as well. At first I wrote them off because of all the star trek references, but products are not notch and customer service is great. Prices are still in the easonable" range, though, as a result of the recession I might expect a price jump. Even if their detection kits doouble, they will still be better priced that Dako. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, March 12, 2009 11:34 AM To: HornHV@archildrens.org; anne.lewin@bms.com; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: Re: [Histonet] Dako All of you fed up with DAKO and thermo et al.....I figured that out along time ago and switched to a new company called Biocare.? And now I have been using them for years.? They still offer that personal touch.? They still do reagent rental agreements.? They still offer discounts based on purchasing.? Hey....I would give them a call. -----Original Message----- From: Horn, Hazel V To: Lewin, Anne ; histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Sent: Thu, 12 Mar 2009 12:17 pm Subject: RE: [Histonet] Dako My purchasing agent notified me of the huge markup of their reagents/antibodies.???I called my rep told them I was unhappy and would have to look elsewhere and they decided to give me the old pricing for a year.? I still don't have it in writing. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way? ? Slot 820 Little Rock, AR???72202 phone???501.364.4240 fax? ? ? ? 501.364.3155 visit us on the web at:? ? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewin, Anne Sent: Thursday, March 12, 2009 10:14 AM To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org Subject: RE: [Histonet] Dako We not only have noticed a huge mark-up on their products, but it is taking longer to receive shipments from them.? Did Dako go through some sort of re-org or had major lay-offs resulting in fewer people having to do more?? Anne >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier >Sent: Thursday, March 12, 2009 11:08 AM >To: histonet@lists.utsouthwestern.edu; jlhowery@yrmc.org >Subject: Re: [Histonet] Dako > >No, but the price of their DAB+ did jump drastically.? I like the >product, but I may have to find an alternative. > >Tom Pier > >>>> "jeff" 03/12/09 9:47 AM >>> >I was wondering if anyone else is being blackmailed by Dako to sign a >contract with them. We have enjoyed our relationship a number of years >buying there product as we need it. We also have changed detection kits >as we where advised. Now they are telling us that if we do not go? with >price per slide they will raise our costs of purchasing our supplies. >Example? EnVision + dual link system last year cost 1,580.70 this year >3,300 and that this will go in effect March 1st ( just talked to the rep >about this Tues) Just 1 example. SOOOO. Has anyone else had this happen >t o them and what did you do????Thanks? Jeff > >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the m essage and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thaas <@t> mhhcc.org Fri Mar 13 12:53:30 2009 From: thaas <@t> mhhcc.org (Tina Haas/mhhcc.org) Date: Fri Mar 13 12:51:33 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F60801F41@msxc06.OSUMC.EDU> Message-ID: Tina Haas, HT, ASCP Section Head, Pathology Department Memorial Hospital and Health Care Center Jasper, IN 47546 Ph#812-482-0291 Fax#812-482-0447 thaas@mhhcc.org please unsubscribe from histonet. thanks! "Whitaker, Bonnie" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/13/2009 01:44 PM To histonet cc Subject RE: [Histonet] Disposal of Formaldehyde I agree. It is up to each state/county/city/municipality or whatever to determine what their regulations are, and facilities must comply with the most stringent set of rules that they are governed by. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Friday, March 13, 2009 1:30 PM To: Marsh, Nannette; rjbuesa@yahoo.com; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I disagree also, it is up to the local wastewater treatment plant to decide if you may dispose of formalin down the drain, they also dictate how much. It is not prohibited across the board.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Friday, March 13, 2009 12:24 PM To: 'rjbuesa@yahoo.com'; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail is for the use of the intended recipient(s) only. The information contained in this communication may be confidential, privileged, or protected by copyright, and may be subject to confidentiality agreements. If you are the intended recipient and you do not wish to receive similar electronic messages from us in future then please respond to the sender to this effect. If you have received this email in error, please notify the sender immediately and then delete it. If you are not the intended recipient, you must not keep, use, disclose, copy or distribute this email without the author's prior permission. From carrolpb <@t> umdnj.edu Fri Mar 13 12:58:52 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Mar 13 12:59:01 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: References: Message-ID: <49BA9EDC.6060305@umdnj.edu> someday, people are gonna learn that theres a self-service unsubscribe link at the end of each and every mail sent from this list ;) Tina Haas/mhhcc.org wrote: > Tina Haas, HT, ASCP > Section Head, Pathology Department > Memorial Hospital and Health Care Center > Jasper, IN 47546 > Ph#812-482-0291 > Fax#812-482-0447 > thaas@mhhcc.org > > > > please unsubscribe from histonet. thanks! > > > > > "Whitaker, Bonnie" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/13/2009 01:44 PM > > To > histonet > cc > > Subject > RE: [Histonet] Disposal of Formaldehyde > > > > > > > I agree. It is up to each state/county/city/municipality or whatever to > determine what their regulations are, and facilities must comply with the > most stringent set of rules that they are governed by. > > Bonnie Whitaker > Clinical Histology Manager > Ohio State University Medical Center > 614.293.5048 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa > Fields > Sent: Friday, March 13, 2009 1:30 PM > To: Marsh, Nannette; rjbuesa@yahoo.com; histonet; Jessica Piche > Subject: RE: [Histonet] Disposal of Formaldehyde > > I disagree also, it is up to the local wastewater treatment plant to > decide > if you may dispose of formalin down the drain, they also dictate how much. > It is not prohibited across the board.. > > Rosa Fields, HT (ASCP) > Gastroenterology Specialties > Histology Supervisor > 4545 R Street > Lincoln, NE 68503 > 402-465-4545 > rfields@gidocs.net > > The information contained in the message and the documents accompanying > this > message contain information that is privileged and confidential and is > intended only for the use of the individual or entity named above. If the > reader of this message is not the intended recipient or the employee or > agent > responsible for delivering it to the intended recipient, you are hereby > notified that any dissemination, distribution or copying of this > communication, other than its return to the sender, is strictly > prohibited. > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, > Nannette > Sent: Friday, March 13, 2009 12:24 PM > To: 'rjbuesa@yahoo.com'; histonet; Jessica Piche > Subject: RE: [Histonet] Disposal of Formaldehyde > > I have to disagree. Although it certainly is not environmentally friendly > to > dispose of formalin down the drain, it is not prohibited. At the hospital > where I worked, a city inspector came to the lab and and designated in > writing how many gallons of formalin could be put in the sewer system per > day > and we had to keep a written log of how many gallons we could dispose of > and > could not go over our amount or we would be fined. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Friday, March 13, 2009 11:27 AM > To: histonet; Jessica Piche > Subject: Re: [Histonet] Disposal of Formaldehyde > > > Jessica: > It is absolutely prohibited, "verboten" to dump formalin into the sewer > system. Try to check out other recommendations by this "consultant" and > take > them with, at least, a grain of salt. That guy does not know what is > talking > about and could get your lab in serious trouble. Ren? J. > > --- On Fri, 3/13/09, Jessica Piche wrote: > > From: Jessica Piche > Subject: [Histonet] Disposal of Formaldehyde > To: "histonet" > Date: Friday, March 13, 2009, 10:11 AM > > > Hi All, > We have a question regarding the disposal of formaldehyde. We were told at > our hospital that a consultant said it was okay to dump formaldehyde down > the > drain. I believe they said it was okay to dump 15 gallons or so a day! We > are > not to fond of this idea and would like to know what everyone else is > doing. > How is everyone disposing of their formaldehyde? We would be especially > interested in what other hospitals in CT are doing. > Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail is for the use of the intended recipient(s) only. The information contained in this communication may be confidential, privileged, or protected by copyright, and may be subject to confidentiality agreements. If you are the intended recipient and you do not wish to receive similar electronic messages from us in future then please respond to the sender to this effect. If you have received this email in error, please notify the sender immediately and then delete it. If you are not the intended recipient, you must not keep, use, disclose, copy or distribute this email without the author's prior permission. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From barbaraaalbert <@t> yahoo.com Fri Mar 13 13:02:32 2009 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Fri Mar 13 13:02:35 2009 Subject: [Histonet] Cox2 antibody Message-ID: <131184.20501.qm@web63708.mail.re1.yahoo.com> Hi all, I'm sending this for the head of our immuno department.? She's asking if anyone has had success with the Cox2 antibody in FFPE human tissue.? I'll pass any replies on to her. Thanks, Barbara Albert UCSF Medical Center San Francisco From vapatpxs <@t> yahoo.com Fri Mar 13 13:02:35 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Fri Mar 13 13:02:41 2009 Subject: [Histonet] blades Message-ID: <550193.24787.qm@web46116.mail.sp1.yahoo.com> I thought the replies would be more "cutting edge". What I do is the most low tech of all, I put the still usable knife in a box (usually the empty box from the slides we use) and then use the blades when needed. Just be careful to keep the pointy part away from your fingers. All histotechs have probably grabbed the wrong end of a blade and shed blood for the lab. Happy Friday, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Fri, 3/13/09, Webb, Dorothy L wrote: > From: Webb, Dorothy L > Subject: [Histonet] blades > To: histonet@lists.utsouthwestern.edu > Date: Friday, March 13, 2009, 4:59 PM > Kind of a "Friday" type of question > inasmuch as it could open some > "cutting" remarks........how do others handle microtome > blades that are > not totally used?? Our situation is that we use the > more expensive > "teflon coated" blades for certain tissues that are more > delicate or > difficult to obtain the most optimal sections and use the > ceramic coated > blades for routine microtomy. Obviously, the techs are > oftentimes going > to have a blade with an area that is still very usable and > the dilemma > is a safety issue as to where to place/store blades that > are not ready > to discard!? Any good ideas???? Appreciate any > "kind" ideas!! > Thanks..... > ________________________________________ > This e-mail and any files transmitted with it are > confidential and are intended solely for the use of the > individual or entity to whom they are addressed. If you are > not the intended recipient or the individual responsible for > delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of > this e-mail is strictly prohibited. > > If you have received this e-mail in error, please > immediately notify the HealthPartners Support Center by > telephone at (952) 967-6600. You will be reimbursed for > reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists..utsouthwestern.edu/mailman/listinfo/histonet > From billodonnell <@t> catholichealth.net Fri Mar 13 13:12:19 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Mar 13 13:12:33 2009 Subject: [Histonet] blades In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA2705635E48@hpes1.HealthPartners.int> Message-ID: Jennifer, Ooooooh, I like that! I'll start doing the same first thing Monday. Funny how such a simple fix was literally sitting in the drawer next to the microtome all along! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 13, 2009 12:28 PM To: Webb, Dorothy L Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] blades I will save a blade in a plastic 5-slide mailer. I usually use it for trimming so that I don't waste a new blade. I label the container with "sharps" info. Jennifer "Webb, Dorothy L" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/13/2009 10:03 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] blades Kind of a "Friday" type of question inasmuch as it could open some "cutting" remarks.......how do others handle microtome blades that are not totally used? Our situation is that we use the more expensive "teflon coated" blades for certain tissues that are more delicate or difficult to obtain the most optimal sections and use the ceramic coated blades for routine microtomy. Obviously, the techs are oftentimes going to have a blade with an area that is still very usable and the dilemma is a safety issue as to where to place/store blades that are not ready to discard! Any good ideas??? Appreciate any "kind" ideas!! Thanks..... ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Mar 13 13:19:59 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Mar 13 13:20:02 2009 Subject: [Histonet] Roche/Genetech Today Buy-out and Lay-offs Message-ID: <608831.17910.qm@web31308.mail.mud.yahoo.com> FYI... This is from Todays Fierce Pharma Report: Today's Top Stories 1. Roche to cut jobs in "California cowboys" deal By Tracy Staton Comment | Forward Deal dissection of the day, No. 1: Now that Genentech has finally conceded to Roche's buyout plans, the postgame analysis begins. As we all know after months of following the possibility of a deal, preserving innovation at Genentech is job one. Next on the priority list comes cost-cutting. But neither of those goals will kick in if shareholders don't tender, so we're hearing plenty about their opinions. Here's a sampling of the predictions, prognositications, and pushy advice: Roche plans to shed more than 1,500 jobs in New Jersey and relocate major operations to California as part of the Genentech takeover. Some 1,600 researchers and scientists will stay behind in Nutley, NJ. But the company is redoing its campus master plan to figure out what to do with all the vacant buildings that are left. And who's first in line for the job cuts? None other than sales and marketing, the Star-Ledger says. After years of partnership, Roche and Genentech are already close. Merging their drug portolios should be a snap, New York Times DealBook says. But their cultures are almost diametrically opposed: Roche is Swiss; trains-running-on-time, precision-watchmaking Swiss. Genentech, as one Silicon Valley venture capitalist put it, "are a bunch of entrepreneurial California cowboys." Get the image? Then you see the problem. Roche execs may be guzzling celebratory champagne (somehow we can't imagine it, but it's possible). But along with its buyout of Genentech's promising pipeline come worries about payer reimbursements. President Obama's push for comparative-effectiveness research could leave some Genentech drugs struggling, BusinessWeek reports. Some previous head-to-head studies have shown the benefits of expensive cancer biologics are small; if that trend continues, the bottom line could suffer. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From jtrejo2 <@t> slu.edu Fri Mar 13 13:20:24 2009 From: jtrejo2 <@t> slu.edu (Julie Trejo) Date: Fri Mar 13 13:26:41 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <926491.92795.qm@web82001.mail.mud.yahoo.com> References: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: I've worked at several places and each one is different on treating formalin. The best one I like is to use the Formalin recycler from "Creative Wastes". It's not electric, just works off of gravity and layers of filters. Less formalin to buy, don't have to buy neutralizers and don't have to dump formalin down the drain. Pour it in and in an hour or so it is ready. Just check the pH and it's good to go. You can either decide on a benchtop or on wheels. I don't work for them, I just really like it. Think Green. Here's the website : http://www.cwsincorp.com/ Julie On Fri, Mar 13, 2009 at 9:11 AM, Jessica Piche wrote: > > Hi All, > We have a question regarding the disposal of formaldehyde. We were told at > our hospital that a consultant said it was okay to dump formaldehyde down > the drain. I believe they said it was okay to dump 15 gallons or so a day! > We are not to fond of this idea and would like to know what everyone else is > doing. How is everyone disposing of their formaldehyde? We would be > especially interested in what other hospitals in CT are doing. > Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 From BFicher <@t> chomp.org Fri Mar 13 13:49:55 2009 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Fri Mar 13 13:50:05 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F60801F41@msxc06.OSUMC.EDU> References: <07732CE52EC3174AB891DE1C62DB4D8F6F9561@GIEXCHANGE.gidocs.net> <3CE20ED86C4A114EBDF3BCE8DEFD8F60801F41@msxc06.OSUMC.EDU> Message-ID: So I guess another analogy would be that's OK to dump any chemical into the ocean even though we know that it kills life. Some countries do this and say it's OK while the more thoughtful ones have strict rules about it. Think about it...Formalin kills, can you assure that it will be dissipated enough in your water system to be safe? What's our part in all of this? As Creatures of our world, we have the insight to do what's correct to save our world, not contribute to it's demise. So it costs a few more dollars to keep it safe...your children and theirs will thank you and appreciate your concern...We have opposable thumbs that makes us different from the other creatures, we can make tools and rationalize danger... R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Friday, March 13, 2009 10:45 AM To: histonet Subject: RE: [Histonet] Disposal of Formaldehyde I agree. It is up to each state/county/city/municipality or whatever to determine what their regulations are, and facilities must comply with the most stringent set of rules that they are governed by. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Friday, March 13, 2009 1:30 PM To: Marsh, Nannette; rjbuesa@yahoo.com; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I disagree also, it is up to the local wastewater treatment plant to decide if you may dispose of formalin down the drain, they also dictate how much. It is not prohibited across the board.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Friday, March 13, 2009 12:24 PM To: 'rjbuesa@yahoo.com'; histonet; Jessica Piche Subject: RE: [Histonet] Disposal of Formaldehyde I have to disagree. Although it certainly is not environmentally friendly to dispose of formalin down the drain, it is not prohibited. At the hospital where I worked, a city inspector came to the lab and and designated in writing how many gallons of formalin could be put in the sewer system per day and we had to keep a written log of how many gallons we could dispose of and could not go over our amount or we would be fined. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 13, 2009 11:27 AM To: histonet; Jessica Piche Subject: Re: [Histonet] Disposal of Formaldehyde Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From sbreeden <@t> nmda.nmsu.edu Fri Mar 13 13:55:48 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Mar 13 13:55:53 2009 Subject: [Histonet] Blades Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6752@nmdamailsvr.nmda.ad.nmsu.edu> And here I've been wondering how to keep my used blade overnight so I can use it the next day for facing... I've been wrapping it in a 4x4 gauze and squirreling it behind my blade dispenser in a cubbyhole in my BalanceBank. I am the only one in my lab, so having someone come across it was not an issue, but I still figured I could come up with something better.Thank you, Jennifer, for the BRILLIANT idea of storing it in a 5-slide mailing container. Give that girl a cig...no, a cookie! Now I have nothing to fume about on this after-the-rain Friday Hour of Fuming! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From akemiat3377 <@t> yahoo.com Fri Mar 13 13:59:50 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Mar 13 13:59:54 2009 Subject: [Histonet] Roche/Genetech Buy-out Message-ID: <854848.85368.qm@web31302.mail.mud.yahoo.com> FYI... This is from Todays Fierce Pharma Report: Today's Top Stories FYI... This is from Todays Fierce Pharma Report: 1. Roche to cut 1,500 jobs in "California cowboys" deal By Tracy Staton Deal dissection of the day, No. 1: Now that Genentech has finally conceded to Roche's buyout plans, the postgame analysis begins. As we all know after months of following the possibility of a deal, preserving innovation at Genentech is job one. Next on the priority list comes cost-cutting. But neither of those goals will kick in if shareholders don't tender, so we're hearing plenty about their opinions. Here's a sampling of the predictions, prognositications, and pushy advice: Roche plans to shed more than 1,500 jobs in New Jersey and relocate major operations to California as part of the Genentech takeover. Some 1,600 researchers and scientists will stay behind in Nutley, NJ. But the company is redoing its campus master plan to figure out what to do with all the vacant buildings that are left. And who's first in line for the job cuts? None other than sales and marketing, the Star-Ledger says. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From godsgalnow <@t> aol.com Fri Mar 13 14:07:35 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Mar 13 14:07:51 2009 Subject: [Histonet] microwave fixation Message-ID: <8CB72261AB34BA5-7C4-747@webmail-mf04.sysops.aol.com> Me again! To those of you that do formalin fixation in the microwave, would you please share your protocol?? I am having issues Roxanne From tkngflght <@t> yahoo.com Fri Mar 13 14:14:15 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Mar 13 14:14:21 2009 Subject: Call your local water authority Re: [Histonet] Disposal of Formaldehyde In-Reply-To: Message-ID: <7740.95447.qm@web50904.mail.re2.yahoo.com> Hi All- ? There is no 'one' answer to this.??Small?reference labs may be under different regs than a 500 bed hospital even if they are in the same ZIP code. ? Talk to your management and gain permission to call the local water and waste authority.? Please go through proper chain of command?before doing?this. ? Then when you talk with the water folks, tell them of your waste type, volumes and request a WRITTEN regulation.? If a hospital is flushing 200,000 gallons of water into the sewers with 15 gallons of 10%NBF mixed in, they MAY be allowed to just wash it down the drain with copious rinsing.? A little lab only dumping 1000 gallons of water a day may not have the same permissions. ? I am going to look into the solution posed by Julie T....no more chemicals into the water supply regardless of what the regs say?helps me?feel better about our future!!? Remember how we used to not think twice about putting our hands into xylene all day??? ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Healthcare?Professionals ~ One GREAT?fit at a time. 281.852.9457? --- On Fri, 3/13/09, Julie Trejo wrote: From: Julie Trejo Subject: Re: [Histonet] Disposal of Formaldehyde To: "Jessica Piche" Cc: "histonet" Date: Friday, March 13, 2009, 11:20 AM I've worked at several places and each one is different on treating formalin. The best one I like is to use the Formalin recycler from "Creative Wastes". It's not electric, just works off of gravity and layers of filters. Less formalin to buy, don't have to buy neutralizers and don't have to dump formalin down the drain. Pour it in and in an hour or so it is ready. Just check the pH and it's good to go. You can either decide on a benchtop or on wheels. I don't work for them, I just really like it. Think Green. Here's the website : http://www.cwsincorp.com/ Julie On Fri, Mar 13, 2009 at 9:11 AM, Jessica Piche wrote: > > Hi All, > We have a question regarding the disposal of formaldehyde. We were told at > our hospital that a consultant said it was okay to dump formaldehyde down > the drain. I believe they said it was okay to dump 15 gallons or so a day! > We are not to fond of this idea and would like to know what everyone else is > doing. How is everyone disposing of their formaldehyde? We would be > especially interested in what other hospitals in CT are doing. > Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Mar 13 14:14:48 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Mar 13 14:14:53 2009 Subject: Call your local water authority Re: [Histonet] Disposal of Formaldehyde In-Reply-To: Message-ID: <305817.98772.qm@web50910.mail.re2.yahoo.com> Hi All- ? There is no 'one' answer to this.??Small?reference labs may be under different regs than a 500 bed hospital even if they are in the same ZIP code. ? Talk to your management and gain permission to call the local water and waste authority.? Please go through proper chain of command?before doing?this. ? Then when you talk with the water folks, tell them of your waste type, volumes and request a WRITTEN regulation.? If a hospital is flushing 200,000 gallons of water into the sewers with 15 gallons of 10%NBF mixed in, they MAY be allowed to just wash it down the drain with copious rinsing.? A little lab only dumping 1000 gallons of water a day may not have the same permissions. ? I am going to look into the solution posed by Julie T....no more chemicals into the water supply regardless of what the regs say?helps me?feel better about our future!!? Remember how we used to not think twice about putting our hands into xylene all day??? ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Healthcare?Professionals ~ One GREAT?fit at a time. 281.852.9457? --- On Fri, 3/13/09, Julie Trejo wrote: From: Julie Trejo Subject: Re: [Histonet] Disposal of Formaldehyde To: "Jessica Piche" Cc: "histonet" Date: Friday, March 13, 2009, 11:20 AM I've worked at several places and each one is different on treating formalin. The best one I like is to use the Formalin recycler from "Creative Wastes". It's not electric, just works off of gravity and layers of filters. Less formalin to buy, don't have to buy neutralizers and don't have to dump formalin down the drain. Pour it in and in an hour or so it is ready. Just check the pH and it's good to go. You can either decide on a benchtop or on wheels. I don't work for them, I just really like it. Think Green. Here's the website : http://www.cwsincorp.com/ Julie On Fri, Mar 13, 2009 at 9:11 AM, Jessica Piche wrote: > > Hi All, > We have a question regarding the disposal of formaldehyde. We were told at > our hospital that a consultant said it was okay to dump formaldehyde down > the drain. I believe they said it was okay to dump 15 gallons or so a day! > We are not to fond of this idea and would like to know what everyone else is > doing. How is everyone disposing of their formaldehyde? We would be > especially interested in what other hospitals in CT are doing. > Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Mar 13 14:15:09 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Mar 13 14:15:15 2009 Subject: Call your local water authority Re: [Histonet] Disposal of Formaldehyde Message-ID: <837865.9995.qm@web50906.mail.re2.yahoo.com> Hi All- ? There is no 'one' answer to this.??Small?reference labs may be under different regs than a 500 bed hospital even if they are in the same ZIP code. ? Talk to your management and gain permission to call the local water and waste authority.? Please go through proper chain of command?before doing?this. ? Then when you talk with the water folks, tell them of your waste type, volumes and request a WRITTEN regulation.? If a hospital is flushing 200,000 gallons of water into the sewers with 15 gallons of 10%NBF mixed in, they MAY be allowed to just wash it down the drain with copious rinsing.? A little lab only dumping 1000 gallons of water a day may not have the same permissions. ? I am going to look into the solution posed by Julie T....no more chemicals into the water supply regardless of what the regs say?helps me?feel better about our future!!? Remember how we used to not think twice about putting our hands into xylene all day??? ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Healthcare?Professionals ~ One GREAT?fit at a time. 281.852.9457? --- On Fri, 3/13/09, Julie Trejo wrote: From: Julie Trejo Subject: Re: [Histonet] Disposal of Formaldehyde To: "Jessica Piche" Cc: "histonet" Date: Friday, March 13, 2009, 11:20 AM I've worked at several places and each one is different on treating formalin. The best one I like is to use the Formalin recycler from "Creative Wastes". It's not electric, just works off of gravity and layers of filters. Less formalin to buy, don't have to buy neutralizers and don't have to dump formalin down the drain. Pour it in and in an hour or so it is ready. Just check the pH and it's good to go. You can either decide on a benchtop or on wheels. I don't work for them, I just really like it. Think Green. Here's the website : http://www.cwsincorp.com/ Julie On Fri, Mar 13, 2009 at 9:11 AM, Jessica Piche wrote: > > Hi All, > We have a question regarding the disposal of formaldehyde. We were told at > our hospital that a consultant said it was okay to dump formaldehyde down > the drain. I believe they said it was okay to dump 15 gallons or so a day! > We are not to fond of this idea and would like to know what everyone else is > doing. How is everyone disposing of their formaldehyde? We would be > especially interested in what other hospitals in CT are doing. > Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sweaver <@t> tvmdl.tamu.edu Fri Mar 13 14:16:50 2009 From: sweaver <@t> tvmdl.tamu.edu (Stephanie Weaver) Date: Fri Mar 13 14:17:09 2009 Subject: [Histonet] IHC stainer Message-ID: <49BA6AD5.A3DC.00A0.0@tvmdl.tamu.edu> I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience with IHC and we typically have a relatively high turnover rate. Therefore, I am hoping to be able to buy an automated stainer. In the past most people on the list seemed to be very happy with the Dako autostainer, but this past week has brought so many bad remarks about Dako's service that I am reconsidering. We probably will not need a high capacity autostainer, but I would like walk-away capability with an easy to use system. It will need to accept other companies reagents, since veterinary infectious disease antibodies aren't often sold by the major companies. Also, cost is an issue and I would like to be able to bargain shop for reagents through other companies. Does anyone have any recommendations, or warnings as to what to avoid? In a related issue, where do other animal tissue people get their antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or FIP? Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Lab From tkngflght <@t> yahoo.com Fri Mar 13 14:43:33 2009 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Mar 13 14:39:09 2009 Subject: Call your local water authority Re: [Histonet] Disposal ofFormaldehyde In-Reply-To: Message-ID: Happy to be corrected~~thanks Ruth! Does anyone have access to the written EPA regs on this--I need to send them to a few people who, like me, are a little behind the times! Cheryl -----Original Message----- From: Yaskovich, Ruth A (NIH/NIDCR) [E] [mailto:ryaskovich@dir.nidcr.nih.gov] Sent: Friday, March 13, 2009 2:28 PM To: 'Cheryl' Subject: RE: Call your local water authority Re: [Histonet] Disposal ofFormaldehyde Cheryl, There is one answer to this the E.P.A. guidelines say nothing down the drain only water. I have been inspected by them in the past. Believe they can enact heavy fines at any time. Ruth N.I.H. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Friday, March 13, 2009 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: Call your local water authority Re: [Histonet] Disposal of Formaldehyde Hi All- ? There is no 'one' answer to this.??Small?reference labs may be under different regs than a 500 bed hospital even if they are in the same ZIP code. ? Talk to your management and gain permission to call the local water and waste authority.? Please go through proper chain of command?before doing?this. ? Then when you talk with the water folks, tell them of your waste type, volumes and request a WRITTEN regulation.? If a hospital is flushing 200,000 gallons of water into the sewers with 15 gallons of 10%NBF mixed in, they MAY be allowed to just wash it down the drain with copious rinsing.? A little lab only dumping 1000 gallons of water a day may not have the same permissions. ? I am going to look into the solution posed by Julie T....no more chemicals into the water supply regardless of what the regs say?helps me?feel better about our future!!? Remember how we used to not think twice about putting our hands into xylene all day??? ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Healthcare?Professionals ~ One GREAT?fit at a time. 281.852.9457? --- On Fri, 3/13/09, Julie Trejo wrote: From: Julie Trejo Subject: Re: [Histonet] Disposal of Formaldehyde To: "Jessica Piche" Cc: "histonet" Date: Friday, March 13, 2009, 11:20 AM I've worked at several places and each one is different on treating formalin. The best one I like is to use the Formalin recycler from "Creative Wastes". It's not electric, just works off of gravity and layers of filters. Less formalin to buy, don't have to buy neutralizers and don't have to dump formalin down the drain. Pour it in and in an hour or so it is ready. Just check the pH and it's good to go. You can either decide on a benchtop or on wheels. I don't work for them, I just really like it. Think Green. Here's the website : http://www.cwsincorp.com/ Julie On Fri, Mar 13, 2009 at 9:11 AM, Jessica Piche wrote: > > Hi All, > We have a question regarding the disposal of formaldehyde. We were told at > our hospital that a consultant said it was okay to dump formaldehyde down > the drain. I believe they said it was okay to dump 15 gallons or so a day! > We are not to fond of this idea and would like to know what everyone else is > doing. How is everyone disposing of their formaldehyde? We would be > especially interested in what other hospitals in CT are doing. > Thanks, > Jessica Piche-Grocki, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtrejo2 <@t> slu.edu Fri Mar 13 14:41:06 2009 From: jtrejo2 <@t> slu.edu (Julie Trejo) Date: Fri Mar 13 14:41:10 2009 Subject: [Histonet] IHC stainer In-Reply-To: <49BA6AD5.A3DC.00A0.0@tvmdl.tamu.edu> References: <49BA6AD5.A3DC.00A0.0@tvmdl.tamu.edu> Message-ID: I suggest using the Leica Bond-Max. I, an HT(ASCP)cm, did not have any IHC experiance, but the Bond-Max makes it happen. Just putting dried slides on, patient info in the computer, slides labeled and then in 3-4 hours, it's all done. Just dehydrate and clear. Very simple and now giving me time to learn IHC and troubleshooting. Some of the antibodies come "Ready to use" from Leica, and we titer others that aren't "Ready to use" (Biocare, Dako etc). I've seen co-workers in the past struggling with immuno's using the Dako, and taking alot of time and still counter-staining by hand. The Bond-Max allows us (just three of us) to do all routine H&E's, special stains, recuts, deepers and Immuno's in one day. Not bad. Julie On Fri, Mar 13, 2009 at 2:16 PM, Stephanie Weaver wrote: > I am in a veterinary diagnostic lab. In the past we have had very few > requests for IHC and have always sent slides out to another lab to perform > IHC as needed. It is time for us to start doing our own and join the modern > age. We have several certified technicians, but none have experience with > IHC and we typically have a relatively high turnover rate. Therefore, I am > hoping to be able to buy an automated stainer. In the past most people on > the list seemed to be very happy with the Dako autostainer, but this past > week has brought so many bad remarks about Dako's service that I am > reconsidering. We probably will not need a high capacity autostainer, but I > would like walk-away capability with an easy to use system. It will need to > accept other companies reagents, since veterinary infectious disease > antibodies aren't often sold by the major companies. Also, cost is an issue > and I would like to be able to bargain shop for reagents through other > companies. Does anyone have any recommendations, or warnings as to what to > avoid? > > In a related issue, where do other animal tissue people get their > antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or > FIP? > > Thanks for the advice! > > > > > Stephanie Weaver > Texas Veterinary Medical Diagnostic Lab > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 From asmith <@t> mail.barry.edu Fri Mar 13 14:41:49 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Mar 13 14:43:07 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <926491.92795.qm@web82001.mail.mud.yahoo.com> References: <926491.92795.qm@web82001.mail.mud.yahoo.com> Message-ID: Formaldehyde can mess up the bacteria that treat the sewage. We used to neutralize our formaldehyde with Richard Allen's "Vytac" and send the results down the drain. Two years ago we were told that we would have to get a license and do quantitative analysis on each batch in order to continue using "Vytac". Now we pay a waste hauler to dispose of our annual gallon of formalin. If we had a lot of it, we would recycle it ourselves. Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 10:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Mar 13 14:45:14 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Mar 13 14:45:47 2009 Subject: [Histonet] IHC stainer In-Reply-To: <49BA6AD5.A3DC.00A0.0@tvmdl.tamu.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B64558C@chi2k3ms01.columbuschildrens.net> Can't speak highly enough of the BondMax form Leica Microsystems. If cost is an issue and you're looking at what to avoid, IMHO avoid anything Ventana Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Friday, March 13, 2009 3:17 PM To: histonet post Subject: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience with IHC and we typically have a relatively high turnover rate. Therefore, I am hoping to be able to buy an automated stainer. In the past most people on the list seemed to be very happy with the Dako autostainer, but this past week has brought so many bad remarks about Dako's service that I am reconsidering. We probably will not need a high capacity autostainer, but I would like walk-away capability with an easy to use system. It will need to accept other companies reagents, since veterinary infectious disease antibodies aren't often sold by the major companies. Also, cost is an issue and I would like to be able to bargain shop for reagents through other companies. Does anyone have any recommendations, or warnings as to what to avoid? In a related issue, where do other animal tissue people get their antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or FIP? Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From pkromund <@t> gundluth.org Fri Mar 13 14:51:14 2009 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Fri Mar 13 14:51:26 2009 Subject: [Histonet] Re: Histonet Digest, Vol 64, Issue 24 In-Reply-To: Message-ID: We store our partically used blades in a centrifuge tube. It works great. Pamela Romundstad Gundersen Lutheran 1910 South Ave No. LaCrosse, WI 54601 608-775-3139 histonet-request@ lists.utsouthwest ern.edu To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject Histonet Digest, Vol 64, Issue 24 03/13/2009 11:58 AM Please respond to histonet@lists.ut southwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. 20 micron resin sections (louise renton) 2. Laura Miller is out of the office. (Laura.Miller@leica-microsystems.com) 3. Re: Tissue processors (Pamela Marcum) 4. Disposal of Formaldehyde (Jessica Piche) 5. Re: Disposal of Formaldehyde (Bryan Watson) 6. RE: Disposal of Formaldehyde (Martin, Gary) 7. RE: Disposal of Formaldehyde (Sherwood, Margaret ) 8. RE: Disposal of Formaldehyde (Bonner, Janet) 9. RE: Disposal of Formaldehyde (Podawiltz, Thomas) 10. dumping formalin (Molinari, Betsy) 11. DI water (LINDA MARGRAF) 12. Re: Disposal of Formaldehyde ( TF ) 13. RE: dumping formalin (Molinari, Betsy) 14. Re: Disposal of Formaldehyde (Rene J Buesa) 15. blades (Webb, Dorothy L) ---------------------------------------------------------------------- Message: 1 Date: Fri, 13 Mar 2009 09:53:14 +0200 From: louise renton Subject: [Histonet] 20 micron resin sections To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, I have a query from a colleague doing research on neuroanatomy as to whether it is possible ( with relative ease) to cut 20mu sections from JB4 resin embedded tissue? Apparently these sections ae to be stained and then used for stereomicroscopy. My experience is not that extensive to be able to answer her, so I would appreciate some advice here best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 2 Date: Fri, 13 Mar 2009 04:02:05 -0600 From: Laura.Miller@leica-microsystems.com Subject: [Histonet] Laura Miller is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 03/13/2009 and will not return until 03/23/2009. I am on vacation. Since I will be out of the country, I will not be checking emails. I will respond to your email when I return on March 23, 2009. Thanks! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 3 Date: Fri, 13 Mar 2009 06:54:36 -0400 (EDT) From: Pamela Marcum Subject: Re: [Histonet] Tissue processors To: Walzer Susan Cc: histonet@lists.utsouthwestern.edu, Margaret Sherwood Message-ID: <2017871265.60386331236941676377.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Content-Type: text/plain; charset=utf-8 I would disagree as I am on my third Shandon in a long career and have never had a problem with any of them. Age and growth in nneds got them. Currently we use a Pathcentre and it has been excellent. If you haven't used one it is hard to comment on how they work and how the company works with you. I have used VIPs and they are good. It is preference and comfort on purchasing a unit. Pam Marcum ----- Original Message ----- From: "Walzer Susan" To: "Margaret Sherwood" , histonet@lists.utsouthwestern.edu Sent: Friday, March 13, 2009 3:17:46 AM GMT -05:00 US/Canada Eastern Subject: RE: Re: [Histonet] Tissue processors I have not heard much good about the Shandon processor. For reliability go with a VIP every time. This comes from MANY years in the field. I have never had problems with one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, March 12, 2009 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processors To all: Sorry to bother the list with a question that has no doubt been addressed in the past, but now that we are looking into possibly replacing our tissue processor, I would like some input. It no longer is covered by a service contract and things are starting to go wrong with it. We have a Hypercenter XL (ThermoShandon). We are a core pathology lab for a research group. Typically we run @ 1000-1500 paraffin blocks/year, so we are looking at a processor that would meet our needs. We have limited space, so something, size-wise, like the Hypercenter XL would be required. What do most people prefer? If you would like to contact me off-list, my email is below. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 13 Mar 2009 07:11:29 -0700 (PDT) From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: histonet Message-ID: <926491.92795.qm@web82001.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) ------------------------------ Message: 5 Date: Fri, 13 Mar 2009 10:25:39 -0400 From: "Bryan Watson" Subject: Re: [Histonet] Disposal of Formaldehyde To: "histonet" , "Jessica Piche" Message-ID: <49BA34A4.5674.0085.1@parkview.com> Content-Type: text/plain; charset=US-ASCII I'm in Indiana. Here, it is up to the city. We dump all of ours down the drain and supposedly the city has ok'd that. They say that our hospital is big enough that all of the water used here will dilute the formalin enough to make it ok. However I had heard, back when I was in school that formalin kills the beneficial bacteria they use at water treatment plants. So I'm opposed to putting it down the drain. . . yet, that's how we do it here. Bryan >>> Jessica Piche 3/13/2009 10:11 AM >>> Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 13 Mar 2009 07:26:35 -0700 From: "Martin, Gary" Subject: RE: [Histonet] Disposal of Formaldehyde To: "Jessica Piche" , "histonet" Message-ID: <6ED9D4252F278841A0593D3D788AF24C04C2F537@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="iso-8859-1" First ... even if the local authorities allow this ... it doesn't make "Green" sense to do it, especially when there are other very workable alternatives. The path we chose was to purchase a very simple gravity feed recycler produced and sold by Creative Waste Solutions (888) 795-8300. Check them out. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 7:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 13 Mar 2009 11:00:33 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] Disposal of Formaldehyde To: "Jessica Piche" , "histonet" Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E238B1@PHSXMB30.partners.org> Content-Type: text/plain; charset="iso-8859-1" We are discouraged from putting most any chemical down the drain. The MWRA (Massachusetts Water Resources Authority) monitors mercury levels, etc. in Boston Harbor (as well as waterways throughout the state) and will issue steep fines if labs are found to be dumping such waste down the drain. Consequently, our in-house Safety Office and our outside waste management company (Triumverate Environmental) monitor our waste. Alcohols, stains, and fixatives are disposed of as hazardous waste. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 10:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 8 Date: Fri, 13 Mar 2009 11:06:53 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] Disposal of Formaldehyde To: "Martin, Gary" , "Jessica Piche" , "histonet" Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F295C@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 The same recycler is used here for us. we are absolutely not allowed to put formaldehyde/formalin or EtOH or Xylene down the sink. If you don't use a recycler, hire a waste company to handle it. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Martin, Gary Sent: Fri 3/13/2009 10:26 AM To: Jessica Piche; histonet Subject: RE: [Histonet] Disposal of Formaldehyde First ... even if the local authorities allow this ... it doesn't make "Green" sense to do it, especially when there are other very workable alternatives. The path we chose was to purchase a very simple gravity feed recycler produced and sold by Creative Waste Solutions (888) 795-8300. Check them out. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 7:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 9 Date: Fri, 13 Mar 2009 11:20:33 -0400 From: "Podawiltz, Thomas" Subject: RE: [Histonet] Disposal of Formaldehyde To: "Sherwood, Margaret " , Jessica Piche , histonet Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BD12@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" We dump very little down the drain. Formalin has not been drained dump since 1988. We used to have it hauled away, because the former administration never got into re-cycling the formalin. What we do today, is too de-formalize the formalin, test it and then drain dumped if it passes. We buy the de-formalizer from Surgipath. We had to get this approved the State and we have to keep our records for 5 years. I keep the original and send a copy to the Director of Hazard Waste. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret [MSHERWOOD@PARTNERS.ORG] Sent: Friday, March 13, 2009 11:00 AM To: Jessica Piche; histonet Subject: RE: [Histonet] Disposal of Formaldehyde We are discouraged from putting most any chemical down the drain. The MWRA (Massachusetts Water Resources Authority) monitors mercury levels, etc. in Boston Harbor (as well as waterways throughout the state) and will issue steep fines if labs are found to be dumping such waste down the drain. Consequently, our in-house Safety Office and our outside waste management company (Triumverate Environmental) monitor our waste. Alcohols, stains, and fixatives are disposed of as hazardous waste. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 10:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 10 Date: Fri, 13 Mar 2009 10:27:54 -0500 From: "Molinari, Betsy" Subject: [Histonet] dumping formalin To: Message-ID: Content-Type: text/plain; charset="us-ascii" We collect ours and the Hazardous Waste Dept of the hospital collects it. Now what they do with it I have no idea. We collect all our waste, fixatives, stains etc. I know of at least 2 facilities in Houston that pour theirs down the drain. I am from Boston I was so pleased to read the steps they have taken. They city spent a lot of money cleaning up the harbor. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ------------------------------ Message: 11 Date: Fri, 13 Mar 2009 10:31:22 -0500 From: "LINDA MARGRAF" Subject: [Histonet] DI water To: Cc: jcampbell@vdxpathology.com Message-ID: <49BA35FB.F783.00DA.0@childrens.com> Content-Type: text/plain; charset="us-ascii" Here's a message from Jennifer....(please respond to the list or her directly.... thanks) Hi everyone, We are getting ready to start running immunos at our lab and we need to install a DI water system. After talking to a friend who recently put in a system at his lab, I realize that there is a lot more to it than just simply putting a hole in the wall and tapping in some DI water. Anways, we are going to start running them by hand but, eventually want to be able to use an automatic stainer. Since the buffers require the use of DI water and it is recommended to it in the pressure cooker or decloaker for heat retrieval, we definitely need to put in a system. We will most likely be starting out running just a handful of stains but, would like to be able to increase that number eventually. Can anyone tell me what type of water you use (ultrapure, Cap type 2 etc), how much water (gpm), how much space in your lab it requires, do you need a certain pressure, and any other helpful tips? I appreciate it. Thank you. Jen Campbell Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
disclosures are prohibited without proper authorization. If you are not the intended recipient, any
disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
applicable privileges related to this information.

------------------------------ Message: 12 Date: Sat, 14 Mar 2009 00:05:26 +0800 From: " TF " Subject: Re: [Histonet] Disposal of Formaldehyde To: " Jessica Piche " , " histonet " Message-ID: <200903140005206905437@foxmail.com> Content-Type: text/plain; charset="utf-8" Hard to say... we perfuse sooooooooooo many animals everyday...several litres for one lab into the sea 2009-03-14 TF ???????????? Jessica Piche ??????????????? 2009-03-13 23:25:02 ???????????? histonet ????????? ????????? [Histonet] Disposal of Formaldehyde ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 13 Mar 2009 11:20:29 -0500 From: "Molinari, Betsy" Subject: RE: [Histonet] dumping formalin To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Margaret, You are right you can take the girl from Boston but not Boston out of the girl. Not to be picky...Bostonian...or has it been changed :0 My entire family is there and I go visit every summer. -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Friday, March 13, 2009 9:37 AM To: Molinari, Betsy Subject: RE: [Histonet] dumping formalin Betsy, Nice to hear from a (former)Bostonite. Although, if you are from Boston, you are always from Boston! You are right: the city did spend a lot of money cleaning up the harbor and all that feeds into it. MGH is right on the Charles River and I'm sure I don't have to tell you how disgusting that was! When I first moved here in 1968 (oops, dating myself!), I went sailing with friends on the Charles (Community Boating). We capsized and it's a wonder we all did not come down with typhoid! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, March 13, 2009 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dumping formalin We collect ours and the Hazardous Waste Dept of the hospital collects it. Now what they do with it I have no idea. We collect all our waste, fixatives, stains etc. I know of at least 2 facilities in Houston that pour theirs down the drain. I am from Boston I was so pleased to read the steps they have taken. They city spent a lot of money cleaning up the harbor. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 14 Date: Fri, 13 Mar 2009 09:27:10 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Disposal of Formaldehyde To: histonet , Jessica Piche Message-ID: <422898.8047.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Jessica: It is absolutely prohibited, "verboten" to dump formalin into the sewer system. Try to check out other recommendations by this "consultant" and take them with, at least, a grain of salt. That guy does not know what is talking about and could get your lab in serious trouble. Ren? J. --- On Fri, 3/13/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Disposal of Formaldehyde To: "histonet" Date: Friday, March 13, 2009, 10:11 AM Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 13 Mar 2009 11:59:29 -0500 From: "Webb, Dorothy L" Subject: [Histonet] blades To: histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635E48@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Kind of a "Friday" type of question inasmuch as it could open some "cutting" remarks.......how do others handle microtome blades that are not totally used? Our situation is that we use the more expensive "teflon coated" blades for certain tissues that are more delicate or difficult to obtain the most optimal sections and use the ceramic coated blades for routine microtomy. Obviously, the techs are oftentimes going to have a blade with an area that is still very usable and the dilemma is a safety issue as to where to place/store blades that are not ready to discard! Any good ideas??? Appreciate any "kind" ideas!! Thanks..... ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the From Keri.Colwell <@t> inspection.gc.ca Fri Mar 13 15:17:47 2009 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Fri Mar 13 15:17:56 2009 Subject: [Histonet] Images of fast red Message-ID: Hi, I'm trying to locate some images of crystal fall out when staining with fast red. Does anyone know where I can look? Keri Colwell Laboratory Technologist CFIA - Lethbridge Laboratory P.O. Box 640 Lethbridge, AB T1J 3Z4 Phone/ T?l?phone (403) 382-5500 ext. 5613/ Facsimile/Telecopier: (403) 381-1202 Email - keri.colwell@inspection.gc.ca Government of Canada/ Gouvernment du Canada www.inspection.gc.ca From akemiat3377 <@t> yahoo.com Fri Mar 13 16:18:10 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Mar 13 16:18:13 2009 Subject: [Histonet] Re: Apogent, Fisher/Thermo In-Reply-To: Message-ID: <547320.45381.qm@web31305.mail.mud.yahoo.com> Hi, FYI Posting from a TBS recent article: Quote: Fisher Scientific Merges with Apogent and then is acquired by Thermo As you have probably heard, Fisher Scientific, one of TBS' premier dealers, first merged with Apogent - an international manufacturing company having several divisions that produce products that compete directly with some of TBS' industry leading reagents and consumables, and, has more recently been acquired by Thermo, the parent company of Shandon. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Fri, 3/13/09, Golden State Acrylic Designs wrote: > From: Golden State Acrylic Designs > Subject: Re: [Histonet] Buy-outs Who owns Who > To: akemiat3377@yahoo.com > Date: Friday, March 13, 2009, 2:03 PM > Apogent Tech was bought out by Thermo who bought Fisher. > > On Fri, Mar 13, 2009 at 1:14 PM, Akemi Allison-Tacha > wrote: > > > > > Happy Friday the 13th Histonet! > > > > Forewarning, this is a little long. There seems to be > a lot of discussion > > as of late, to all the buy outs, and who owns who. I > have been following > > this trend for several years with great interest. It > is amazing how > > pricing, customer support and service, varies from one > company to another, > > even though they are owned by the same parent company. > I can certainly > > understand and relate to the frustration and anger of > the end-user. There is > > so much disconnection within the companies! We need a > score card to keep up > > with all the changes! I maybe wrong, but I don't > think the sales reps even > > know the ownership tree. I put a tree at the end of > this correspondence > > that I think is correct. > > > > I recently had a conservation with a HR rep from > Thermo/Fisher, and she > > didn't even know who Peter Scheu was. Pretty > telling of the knowledge out > > there. HR should at least know who is at the top of > the chain! > > > > Quote: Peter Scheu has been Group President, Clinical > Diagnostics of > > Apogent Technologies Inc. since September 2000. Mr. > Scheu served as the > > President of Richard-Allan Scientific Company > (?Richard-Allan?) from 1997 to > > 2000 and as Executive Vice President of Richard-Allan > from 1995 to 1997. > > > > Let's dispell the confusion and please correct me > if I am wrong. > > > > The ownership trees: > > Parent Company: Apogent Technologies Inc > > Thermo/Fisher > > Richard Allan > > Microm > > Lab Vision > > Neomarkers Antibodies > > > > Parent Company: Danaher > > Leica Microsystems > > Vision Biosystems (Bond) > > Novacastra > > NEW-Suripath > > NEW-McCormick > > > > > > Regards, > > Akemi > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > President > > Phoenix Lab Consulting > > Specializing in Histology, SS, IHC, & TMA > > Tele: (425) 941-4287 > > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tkngflght <@t> yahoo.com Fri Mar 13 16:27:16 2009 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Mar 13 16:22:53 2009 Subject: [Histonet] EPA & your local water authority: a summary and links to the source docs In-Reply-To: Message-ID: <295FAEE5D8844A868B7E5CABB038BC7A@CHERYLSLAPTOP> The EPA governs the environment. There are two major Federal EPA Regs for water: Clean Water Act (CWA) of 1972 & amendments in 1972. Second is the Safe Drinking Water Act (SWDA) with Ammendments dated 1996. The first governs discharge into navigable waters and surface water contamination concerns for industry. They WILL fine the snot out of anyone who breeches these statutes. However--Each State manages the enforcement of their own water treatment programs as long as they aren't in direct conflict with the Fed EPA regs. The second act has more to do with potable water in bottles and when it comes out of your faucet at home. PLEASE NOTE these regs are concerned with direct runoff into larger bodies of water and consumable drinking water, respectively, and there are permits for exceptions. Local waste from healthcare facilities does NOT go into storm sewers with egress to large bodies of water. It goes through your local water processing plant and these are regulated on a local and state level. There are separate regs for healthcare facilities and permits available on a local level for exceptions to the written regs for most industries. If your facility has a permit, you MAY be allowed to pour certain chemicals down the drain--or not. Thus the need to check with your institution's policies and follow proper channels into the inquiry with your local water authority. http://www.epa.gov/regulations/bizsector/healthcare.html Regs by state: http://nlquery.epa.gov/epasearch/epasearch?typeofsearch=area&querytext=waste +water&fld=opeihome%2C+lawsregs%2C+epafiles&areaname=Search+EPA+laws%2C+regu lations%2C+guidance%2C+and+dockets&areacontacts=http%3A%2F%2Fwww.epa.gov%2Fe pahome%2Fcomments3.htm&areasearchurl=&result_template=epafiles_default.xsl&f ilter=samplefilt.hts Summary of the CWA: 33 U.S.C. ?1251 et seq. (1972) The Clean Water Act (CWA) establishes the basic structure for regulating discharges of pollutants into the waters of the United States and regulating quality standards for surface waters. The basis of the CWA was enacted in 1948 and was called the Federal Water Pollution Control Act, but the Act was significantly reorganized and expanded in 1972. "Clean Water Act" became the Act's common name with amendments in 1977. Under the CWA, EPA has implemented pollution control programs such as setting wastewater standards for industry. We have also set water quality standards for all contaminants in surface waters. The CWA made it unlawful to discharge any pollutant from a point source into navigable waters, unless a permit was obtained. EPA's National Pollutant Discharge Elimination System (NPDES) permit program controls discharges. Point sources are discrete conveyances such as pipes or man-made ditches. Individual homes that are connected to a municipal system, use a septic system, or do not have a surface discharge do not need an NPDES permit; however, industrial, municipal, and other facilities must obtain permits if their discharges go directly to surface waters. From akemiat3377 <@t> yahoo.com Fri Mar 13 16:25:37 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Mar 13 16:25:40 2009 Subject: [Histonet] Fw: RE: Back to the Lab in SF Message-ID: <432869.48214.qm@web31306.mail.mud.yahoo.com> Hi Doug and Histonet, Thanks for the clarification on the company tree. You are absolutely correct! Have a great weekend fellow histonet subscribers! As for me, I'm packing and heading back to the SF Bay Area. I have accepted a position as a Histology Manager for a private reference lab. My heart has always been in the lab and patient care. Wish me luck, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Fri, 3/13/09, Akemi Allison-Tacha wrote: > From: Akemi Allison-Tacha > Subject: RE: [Histonet] Buy-outs Who owns Who > To: "Doug Showers" > Date: Friday, March 13, 2009, 1:56 PM > Hi Doug, > > Thanks for the clarification. You are absolutely correct! > > Have a great weekend fellow histonet subscribers! As for > me, I'm packing and heading back to the SF Bay Area. I > have accepted a position as a Histology Manager for a > private reference lab. My heart has always been in the lab > and patient care. > > Wish me luck, > Akemi > > Akemi Allison-Tacha BS, HT (ASCP) HTL > President > Phoenix Lab Consulting > Specializing in Histology, SS, IHC, & TMA > Tele: (425) 941-4287 > E-Mail: akemiat3377@yahoo.com > > > --- On Fri, 3/13/09, Doug Showers > wrote: > > > From: Doug Showers > > Subject: RE: [Histonet] Buy-outs Who owns Who > > To: akemiat3377@yahoo.com > > Date: Friday, March 13, 2009, 12:33 PM > > You certainly do need a program to keep track of who > is who. > > Didn't Fisher > > Scientific purchase Apogent and the Thermo purchased > Fisher > > the next year to > > form Thermo Fisher Scientific? If so then Thermo > should be > > the parent > > company. > > > > Doug Showers, MS, HT > > Histology Manager > > ProPath > > 8267 Elmbrook Dr. Suite 100 > > Dallas, TX 75247 > > 214-237-1680 > > 214-422-3083 Mobile > > 214-237-1706 FAX > > > > > > To learn more about ProPath, please visit > > http://www.ProPathLab.com > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On > > Behalf Of Akemi > > Allison-Tacha > > Sent: Friday, March 13, 2009 12:15 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Buy-outs Who owns Who > > > > > > Happy Friday the 13th Histonet! > > > > Forewarning, this is a little long. There seems to be > a > > lot of discussion as > > of late, to all the buy outs, and who owns who. I > have > > been following this > > trend for several years with great interest. It is > amazing > > how pricing, > > customer support and service, varies from one company > to > > another, even though > > they are owned by the same parent company. I can > certainly > > understand and > > relate to the frustration and anger of the end-user. > There > > is so much > > disconnection within the companies! We need a score > card > > to keep up with all > > the changes! I maybe wrong, but I don't think the > > sales reps even know the > > ownership tree. I put a tree at the end of this > > correspondence that I think > > is correct. > > > > I recently had a conservation with a HR rep from > > Thermo/Fisher, and she > > didn't even know who Peter Scheu was. Pretty > telling > > of the knowledge out > > there. HR should at least know who is at the top of > the > > chain! > > > > Quote: Peter Scheu has been Group President, Clinical > > Diagnostics of Apogent > > Technologies Inc. since September 2000. Mr. Scheu > served as > > the President of > > Richard-Allan Scientific Company > > ("Richard-Allan") from 1997 to 2000 and as > > Executive Vice President of Richard-Allan from 1995 to > > 1997. > > > > Let's dispell the confusion and please correct me > if I > > am wrong. > > > > The ownership trees: > > Parent Company: Apogent Technologies Inc > > Thermo/Fisher > > Richard Allan > > Microm > > Lab Vision > > Neomarkers Antibodies > > > > Parent Company: Danaher > > Leica Microsystems > > Vision Biosystems (Bond) > > Novacastra > > NEW-Suripath > > NEW-McCormick > > > > > > Regards, > > Akemi > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > President > > Phoenix Lab Consulting > > Specializing in Histology, SS, IHC, & TMA > > Tele: (425) 941-4287 > > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Mar 13 17:41:47 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Mar 13 17:41:49 2009 Subject: [Histonet] back to the lab in SF Message-ID: <455915.64129.qm@web31301.mail.mud.yahoo.com> Hi All, I have been receiving numerous inquires as to why I have left PhenoPath. I appreciate your inquires and congratulations. I am going to give a blanket reply, since I have time constraints. PhenoPath is a great company and I have the highest regard for Dr's. Allen Gown, Steve Kussick, and the rest of the staff. Sometimes there is a bigger plan in the cards for us, and my talents are more suited for the laboratory and direct patient care. I was offered a wonderful job in Los Gatos, CA, which is 30 minutes from Santa Cruz, and an hour from Carmel and the wine country. I love the Bay Area. I lived there for 8 years while working for Biocare as the Director of Histology, and I have a lot of friends there, plus weather is wonderful, and it's like going home! Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From gvdobbin <@t> ihis.org Fri Mar 13 19:56:57 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Mar 13 19:57:28 2009 Subject: [Histonet] IHC stainer Message-ID: I echo everything Julie said! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Julie Trejo 3/13/2009 4:41:06 PM >>> I suggest using the Leica Bond-Max. I, an HT(ASCP)cm, did not have any IHC experiance, but the Bond-Max makes it happen. Just putting dried slides on, patient info in the computer, slides labeled and then in 3-4 hours, it's all done. Just dehydrate and clear. Very simple and now giving me time to learn IHC and troubleshooting. Some of the antibodies come "Ready to use" from Leica, and we titer others that aren't "Ready to use" (Biocare, Dako etc). I've seen co-workers in the past struggling with immuno's using the Dako, and taking alot of time and still counter-staining by hand. The Bond-Max allows us (just three of us) to do all routine H&E's, special stains, recuts, deepers and Immuno's in one day. Not bad. Julie On Fri, Mar 13, 2009 at 2:16 PM, Stephanie Weaver wrote: > I am in a veterinary diagnostic lab. In the past we have had very few > requests for IHC and have always sent slides out to another lab to perform > IHC as needed. It is time for us to start doing our own and join the modern > age. We have several certified technicians, but none have experience with > IHC and we typically have a relatively high turnover rate. Therefore, I am > hoping to be able to buy an automated stainer. In the past most people on > the list seemed to be very happy with the Dako autostainer, but this past > week has brought so many bad remarks about Dako's service that I am > reconsidering. We probably will not need a high capacity autostainer, but I > would like walk-away capability with an easy to use system. It will need to > accept other companies reagents, since veterinary infectious disease > antibodies aren't often sold by the major companies. Also, cost is an issue > and I would like to be able to bargain shop for reagents through other > companies. Does anyone have any recommendations, or warnings as to what to > avoid? > > In a related issue, where do other animal tissue people get their > antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or > FIP? > > Thanks for the advice! > > > > > Stephanie Weaver > Texas Veterinary Medical Diagnostic Lab > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Department of Dermatology 314-256-3413 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From gvdobbin <@t> ihis.org Fri Mar 13 19:57:48 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Mar 13 19:58:26 2009 Subject: [Histonet] IHC stainer Message-ID: I guess I would have to echo that as well! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Houston, Ronald" 3/13/2009 4:45:14 PM >>> Can't speak highly enough of the BondMax form Leica Microsystems. If cost is an issue and you're looking at what to avoid, IMHO avoid anything Ventana Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Friday, March 13, 2009 3:17 PM To: histonet post Subject: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience with IHC and we typically have a relatively high turnover rate. Therefore, I am hoping to be able to buy an automated stainer. In the past most people on the list seemed to be very happy with the Dako autostainer, but this past week has brought so many bad remarks about Dako's service that I am reconsidering. We probably will not need a high capacity autostainer, but I would like walk-away capability with an easy to use system. It will need to accept other companies reagents, since veterinary infectious disease antibodies aren't often sold by the major companies. Also, cost is an issue and I would like to be able to bargain shop for reagents through other companies. Does anyone have any recommendations, or warnings as to what to avoid? In a related issue, where do other animal tissue people get their antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or FIP? Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From pmarcum <@t> vet.upenn.edu Fri Mar 13 20:49:38 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Mar 13 20:49:43 2009 Subject: [Histonet] IHC stainer In-Reply-To: Message-ID: <1275753817.60818181236995378105.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Just be sure it is an open system that will allow you to use any primary antibody you need and secondary antibody not just a secondary kit the company provides. You will need to confirm that if you are using a chicken primary for instance, you can get and use a secondary link that will work with the chicken primary to get your staining reaction. Be very careful that it allows you flexibilty and is not designed for clinical standard usage with no ability to truly allow you the range required for animal work. I work with animal and it is hard to find everyhting you need and not all "standard" antibodies and systems will work across species. Good Luck, Pam marcum ----- Original Message ----- From: "Greg Dobbin" To: histonet@lists.utsouthwestern.edu, "Ronald Houston" , sweaver@tvmdl.tamu.edu Sent: Friday, March 13, 2009 8:57:48 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] IHC stainer I guess I would have to echo that as well! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Houston, Ronald" 3/13/2009 4:45:14 PM >>> Can't speak highly enough of the BondMax form Leica Microsystems. If cost is an issue and you're looking at what to avoid, IMHO avoid anything Ventana Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Friday, March 13, 2009 3:17 PM To: histonet post Subject: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience with IHC and we typically have a relatively high turnover rate. Therefore, I am hoping to be able to buy an automated stainer. In the past most people on the list seemed to be very happy with the Dako autostainer, but this past week has brought so many bad remarks about Dako's service that I am reconsidering. We probably will not need a high capacity autostainer, but I would like walk-away capability with an easy to use system. It will need to accept other companies reagents, since veterinary infectious disease antibodies aren't often sold by the major companies. Also, cost is an issue and I would like to be able to bargain shop for reagents through other companies. Does anyone have any recommendations, or warnings as to what to avoid? In a related issue, where do other animal tissue people get their antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or FIP? Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Sat Mar 14 10:31:20 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Sat Mar 14 10:31:39 2009 Subject: [Histonet] microwave fixation In-Reply-To: References: <8CB72261AB34BA5-7C4-747@webmail-mf04.sysops.aol.com> Message-ID: <8CB72D10F43ED32-DCC-24AD@WEBMAIL-DG09.sim.aol.com> I go to the histonet because other techs have experience with different types of tissue and have tried everything to finally get the result.? Most vendors cannot offer?the same kind of advice and suggestions.? No offense but I have tried your microwave with another rep and it wasn't what I was looking for.? I am quite happy with the microwaves that I have, but am now processing other tissues in them.? I have used the histonet for years for advice and suggestions.? Because if you are having a problem with something or can't get something to work right, chances are someone on the histonet has been there and figured it out. That is the purpose of the histonet...to get advice from others when having a problem and to give advice when you have the answers. Roxanne -----Original Message----- From: Jes Strong To: godsgalnow@aol.com Cc: Michelle Rederick Sent: Sat, 14 Mar 2009 11:17 am Subject: RE: [Histonet] microwave fixation Good Morning, I find it interesting that you continually go to the HistoNet for support on your microwave processor instead of the manufacturer of the unit. If you would be interested in upgrading to a Milestone microwave processor, we would be happy to demonstrate to you on site why we are the world leaders in that technology with 3 full time Histotechnicians, with many years of microwave processing experience, at your service to help you maximize your tissue processing. If you would be interested in trying out our microwave processors which were designed and built specifically for laboratory use, unlike the others which are converted kitchen microwave ovens, please let me know and we will be happy to put things in motion so that you can experience consistent high quality microwave processing. Please don't hesitate to contact me if you have any questions, Jes Strong Milestone Medical (847) 323-8373 jes@milestonemed.com www.milestonemed.com Helping Patients -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Friday, March 13, 2009 2:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave fixation Me again! To those of you that do formalin fixation in the microwave, would you please share your protocol?? I am having issues Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Sat Mar 14 13:48:24 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Sat Mar 14 13:48:30 2009 Subject: [Histonet] 20 micron resin sections In-Reply-To: References: Message-ID: Thanks all, This is more or less what i thought, that 20mu sections would prove to be difficult and not all that feasible. So, I wait with bated breath for the protocol from the researcher............ On 3/13/09, Peggy Bisher wrote: > > One of the labs here use JB4 to section Zebrafish. I sent your question to > them to see if they could help you out. Here is their response: > > The consensus in the lab (Kari and I) is that no, probably not. The size > would probably shred the section and chip it to where they would be uneven, > etc. Probably cryo or vibratome would be best. > > They routinely cut their sections between 2-5 microns. > > Good luck to you! > > Cheers, > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu > > > > > > On 3/13/09 3:53 AM, "louise renton" wrote: > > > Hi all, > > > > I have a query from a colleague doing research on neuroanatomy as to > whether > > it is possible ( with relative ease) to cut 20mu sections from JB4 resin > > embedded tissue? Apparently these sections ae to be stained and then used > > for stereomicroscopy. My experience is not that extensive to be able to > > answer her, so I would appreciate some advice here > > best regards-- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From mtc205 <@t> Lehigh.EDU Sat Mar 14 21:56:45 2009 From: mtc205 <@t> Lehigh.EDU (Matthew T Close) Date: Sat Mar 14 21:56:48 2009 Subject: [Histonet] re: 20 micron jb-4 resin sections Message-ID: <456930.1237085805428.JavaMail.mtc205@lehigh.edu> I've never cut 20 micron sections with JB-4, but my guess is that 20 micron sections are possible as they are with some of the other resins. What type of microtome/knife will be used? -Matt --------------------------------- Matthew T. Close Lehigh University Department of Biological Sciences From pruegg <@t> ihctech.net Sun Mar 15 12:05:21 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Mar 15 12:05:28 2009 Subject: [Histonet] IHC stainer In-Reply-To: <1275753817.60818181236995378105.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> References: <1275753817.60818181236995378105.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Message-ID: <854584B5780149D884938E2198F1622B@prueggihctechlt> I have for years worked with animal and human IHC and would have said in the past that the Dako open system autostainer was the way to go with the most flexibility, I still have the old one and use it all the time because you can absolutely use what ever reagent you want and there are way cheaper ways to go than buying these from Dako, Ventana, Leica, or BioCare, or the others. Now that Dako has gone with the more closed system like Ventana I cannot recommend them for open use anymore, a big mistake by Dako in my opinion. The Leica Bond instrument may be an option. They have a research software version that allows you to use open containers and fill them with whose ever reagents you want, BioCare also has an open system new on the market. Sometimes I wonder if in research IHC if it might not be a lot cheaper and we would have better control and understand more about what we are doing if we went back to manual IHC. For the Vet. Lab wanting to start their own IHC work with not a lot of volume and untrained techs, I would definitely want my techs to learn by doing manual staining before I put some instrument in there that will teach them more about how to use the computer software than anything about IHC, but that is just the opinion of an old school gal who sees that there are a lot of people doing critical IHC work who do not understand what they are doing. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Friday, March 13, 2009 7:50 PM To: Greg Dobbin Cc: histonet@lists.utsouthwestern.edu; sweaver@tvmdl.tamu.edu; Ronald Houston Subject: Re: [Histonet] IHC stainer Just be sure it is an open system that will allow you to use any primary antibody you need and secondary antibody not just a secondary kit the company provides. You will need to confirm that if you are using a chicken primary for instance, you can get and use a secondary link that will work with the chicken primary to get your staining reaction. Be very careful that it allows you flexibilty and is not designed for clinical standard usage with no ability to truly allow you the range required for animal work. I work with animal and it is hard to find everyhting you need and not all "standard" antibodies and systems will work across species. Good Luck, Pam marcum ----- Original Message ----- From: "Greg Dobbin" To: histonet@lists.utsouthwestern.edu, "Ronald Houston" , sweaver@tvmdl.tamu.edu Sent: Friday, March 13, 2009 8:57:48 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] IHC stainer I guess I would have to echo that as well! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Houston, Ronald" 3/13/2009 4:45:14 PM >>> Can't speak highly enough of the BondMax form Leica Microsystems. If cost is an issue and you're looking at what to avoid, IMHO avoid anything Ventana Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Friday, March 13, 2009 3:17 PM To: histonet post Subject: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience with IHC and we typically have a relatively high turnover rate. Therefore, I am hoping to be able to buy an automated stainer. In the past most people on the list seemed to be very happy with the Dako autostainer, but this past week has brought so many bad remarks about Dako's service that I am reconsidering. We probably will not need a high capacity autostainer, but I would like walk-away capability with an easy to use system. It will need to accept other companies reagents, since veterinary infectious disease antibodies aren't often sold by the major companies. Also, cost is an issue and I would like to be able to bargain shop for reagents through other companies. Does anyone have any recommendations, or warnings as to what to avoid? In a related issue, where do other animal tissue people get their antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or FIP? Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Mar 15 12:08:51 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Mar 15 12:08:57 2009 Subject: [Histonet] 20 micron resin sections In-Reply-To: References: Message-ID: <0DD4015214B84B7A8F8866E69D7C6FA4@prueggihctechlt> I have cut 20 micron sections of GMA resin using tungsten carbide knives, the cutting is not so difficult (depending on what you are cutting ie calcified bone) as adhering the thick sections to a slide. Thinner sections will stick to + slides a lot better than the thick sections will. So unless they are planning on float staining this will be an obstacle. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Saturday, March 14, 2009 12:48 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 20 micron resin sections Thanks all, This is more or less what i thought, that 20mu sections would prove to be difficult and not all that feasible. So, I wait with bated breath for the protocol from the researcher............ On 3/13/09, Peggy Bisher wrote: > > One of the labs here use JB4 to section Zebrafish. I sent your question to > them to see if they could help you out. Here is their response: > > The consensus in the lab (Kari and I) is that no, probably not. The size > would probably shred the section and chip it to where they would be uneven, > etc. Probably cryo or vibratome would be best. > > They routinely cut their sections between 2-5 microns. > > Good luck to you! > > Cheers, > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu > > > > > > On 3/13/09 3:53 AM, "louise renton" wrote: > > > Hi all, > > > > I have a query from a colleague doing research on neuroanatomy as to > whether > > it is possible ( with relative ease) to cut 20mu sections from JB4 resin > > embedded tissue? Apparently these sections ae to be stained and then used > > for stereomicroscopy. My experience is not that extensive to be able to > > answer her, so I would appreciate some advice here > > best regards-- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Mar 15 12:19:31 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Mar 15 12:19:36 2009 Subject: [Histonet] Images of fast red In-Reply-To: References: Message-ID: <4A681964BDA24E9496E617E957674339@prueggihctechlt> Do you mean precipitating of the fast red? I see that occasionally especially with certain Fast Red reagents, uneven and clear red precipitate on the slide as well as the tissue. I might have some pictures of that I could share with you, not sure. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Friday, March 13, 2009 2:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Images of fast red Hi, I'm trying to locate some images of crystal fall out when staining with fast red. Does anyone know where I can look? Keri Colwell Laboratory Technologist CFIA - Lethbridge Laboratory P.O. Box 640 Lethbridge, AB T1J 3Z4 Phone/ T?l?phone (403) 382-5500 ext. 5613/ Facsimile/Telecopier: (403) 381-1202 Email - keri.colwell@inspection.gc.ca Government of Canada/ Gouvernment du Canada www.inspection.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Sun Mar 15 22:20:56 2009 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Sun Mar 15 22:21:01 2009 Subject: [Histonet] Dako In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE67EB4748@exchange.propathlab.com> References: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu> <82C7248978CB50469FD6BA68EBBEFE67EB4748@exchange.propathlab.com> Message-ID: Those of us in veterinary medicine diagnostics are really hung out to dry with Dako's price increases and push to make us accept the new Flex system. In my institution, there is not a separate charge (nor reimbursement) for IHC slides done for an autopsy (necropsy) or in-house surgical biopsy; it's rolled into the overall pathology charge. (There is no insurance nor medicare to fund diagnostic testing for animals.) Yet, IHC is imperative for a correct and thorough diagnosis, whether it be for tumors or infectious diseases, just like in human medicine. My lab performs about 125 different IHC tests, all done on Dako autostainers, and most of the tumor antibodies are purchased from Dako. Do I want to replace Dako's products with other vendors' items and have to redevelop, optimize, and rewrite all those SOPs? No Way! But I can't afford Dako anymore and am starting to look into what the others have to sell me. Jan Shivers On Mar 12 2009, Doug Showers wrote: >Claire, > One of the things that irritates me about some companies is their > tendency to > charge for their products based solely on what we get reimbursed rather > than >being happy with making a healthy profit based on their expenses. > >Doug Showers, MS, HT >Histology Manager > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > >Sent: Thursday, March 12, 2009 2:23 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Dako > >Why do I keep feeling more and more like these guys are the 'contractors', > and we are the 'government'. i.e. $50 screwdrivers come to mind... I > think we >need to start looking into these guys like others are looking into the >pharmaceutical market pricing. They think they have us over a barrel. I say >we put them in the barrel and pitch them over the falls. (the higher the > better) How about paying for their goods based on how much we get > reimbursed >by insurance/midicare/etc for the testing using their products. Although I >have to say my little experience with immunos (on derm frozen sections, no > less) I was really happy with Biocare. They sent tech specialists to help > out > with protocols and troubleshooting even though we didn't even know if we > were >even going to do the testing at all. Customer service was always great and > very patient. They even transfered me to a specialist if they didn't know > the >answers! Even their kits were compatable with other antibodies, and I was >using predilutes. Never failed me, and improved the staining of the other >brand antibodies when their own kits didn't work. > Sorry. Tons of stress and migranes the past few days. I might be on the > verge >of going Histo if I'm not careful. Is it Friday yet? >Claire > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Pier >Sent: Thu 3/12/2009 1:21 PM >To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; >billodonnell@catholichealth.net; histonet@lists.utsouthwestern.edu; >jlhowery@yrmc.org >Subject: RE: [Histonet] Dako > > > >I've had great luck with Biocare as well. They've been my primary supplier >for detection kits and ancillaries since 2003. I'm probably going to check >them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 >mL. > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting <@t> geisinger.edu Sun Mar 15 23:09:35 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Sun Mar 15 23:09:47 2009 Subject: [Histonet] help with muscle stains Message-ID: <49BD98BF.2B7F.00C9.0@geisinger.edu> Would anyone like to share their procedures for Cytochrome oxidase(COX), Succinic dehydrogenase (SDH) and NADH-TR with me? I have old, old ones but they are sketchy. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From ian.montgomery <@t> bio.gla.ac.uk Mon Mar 16 04:20:49 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Mon Mar 16 04:21:45 2009 Subject: FW: [Histonet] help with muscle stains Message-ID: <96B18E0670004F249761B55D7180DDC2@IBLS.GLA.AC.UK> Angela, Martin, T.P. et al 1988, Am. J. Physiol. 255 C43-50 I use this published technique for SD and GPD, works a treat. If you cannot get the journal let me know and I'll forward the protocol. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: 16 March 2009 04:10 To: histonet Subject: [Histonet] help with muscle stains Would anyone like to share their procedures for Cytochrome oxidase(COX), Succinic dehydrogenase (SDH) and NADH-TR with me? I have old, old ones but they are sketchy. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dfrederi <@t> aerotek.com Mon Mar 16 07:07:17 2009 From: dfrederi <@t> aerotek.com (Frederick, David) Date: Mon Mar 16 07:08:22 2009 Subject: [Histonet] Permanent Job Openings with Medical Diagnostic Company in New Jersey Message-ID: Good morning, I am hiring for several permanent positions with a growing medical diagnostic company here in Central New Jersey. My client has several IMMEDIATE openings for Histology Technicians who have experience with Gram Staining, Section Cutting and Paraffin Embedding. Hands-on experience with Histology Techniques (H&E) is required. My client is rapidly expanding in 2009 and expects to grow their market share and revenues in several key diagnostic areas. These openings are for immediate direct hire and my client is looking to interview ASAP. They also have openings for FISH Technologists as well as Immunohistochemists. Unfortunately, relocation assistance is not available. Though these particular positions are permanent, we do also place individuals in contract and contract-to-hire positions. If you are interested, please respond with an updated Word version of your resume. If you know anyone who might be a fit for these positions, please feel free to forward this email along to them. If you would like, I will add you to a network of individuals here in New Jersey that I will email with new openings as they arise as well. If you are looking for other opportunities, please feel free to reach out to me as I work with many of the medical device / biotech / pharmaceutical companies in Central NJ. Best regards, David David Frederick Aerotek Scientific 371 Hoes Lane, Suite 203, Piscataway, NJ 08854 * Work: 732-447-1187 * Fax: 732-447-1660 / 1661 * E-mail: dfrederi@aerotek.com Who do we know in common? ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From pruegg <@t> ihctech.net Mon Mar 16 07:37:47 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Mar 16 07:37:52 2009 Subject: [Histonet] Dako In-Reply-To: References: <49B90C50020000DF000161A1@gwmail.medicine.wisc.edu><82C7248978CB50469FD6BA68EBBEFE67EB4748@exchange.propathlab.com> Message-ID: Jan, I understand but you might be surprised at how easy it is to make your own buffers, find cheaper diluents and protein blocks, plus cheaper detection reagents that perform as good or better than the ones you are paying so much for, without changing your protocols. We have been having this discussion on the Nsh IHC Resource Group, you can join online at www.ihcrg.org Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shive003@umn.edu Sent: Sunday, March 15, 2009 9:21 PM To: Doug Showers Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Those of us in veterinary medicine diagnostics are really hung out to dry with Dako's price increases and push to make us accept the new Flex system. In my institution, there is not a separate charge (nor reimbursement) for IHC slides done for an autopsy (necropsy) or in-house surgical biopsy; it's rolled into the overall pathology charge. (There is no insurance nor medicare to fund diagnostic testing for animals.) Yet, IHC is imperative for a correct and thorough diagnosis, whether it be for tumors or infectious diseases, just like in human medicine. My lab performs about 125 different IHC tests, all done on Dako autostainers, and most of the tumor antibodies are purchased from Dako. Do I want to replace Dako's products with other vendors' items and have to redevelop, optimize, and rewrite all those SOPs? No Way! But I can't afford Dako anymore and am starting to look into what the others have to sell me. Jan Shivers On Mar 12 2009, Doug Showers wrote: >Claire, > One of the things that irritates me about some companies is their > tendency to > charge for their products based solely on what we get reimbursed rather > than >being happy with making a healthy profit based on their expenses. > >Doug Showers, MS, HT >Histology Manager > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > >Sent: Thursday, March 12, 2009 2:23 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Dako > >Why do I keep feeling more and more like these guys are the 'contractors', > and we are the 'government'. i.e. $50 screwdrivers come to mind... I > think we >need to start looking into these guys like others are looking into the >pharmaceutical market pricing. They think they have us over a barrel. I say >we put them in the barrel and pitch them over the falls. (the higher the > better) How about paying for their goods based on how much we get > reimbursed >by insurance/midicare/etc for the testing using their products. Although I >have to say my little experience with immunos (on derm frozen sections, no > less) I was really happy with Biocare. They sent tech specialists to help > out > with protocols and troubleshooting even though we didn't even know if we > were >even going to do the testing at all. Customer service was always great and > very patient. They even transfered me to a specialist if they didn't know > the >answers! Even their kits were compatable with other antibodies, and I was >using predilutes. Never failed me, and improved the staining of the other >brand antibodies when their own kits didn't work. > Sorry. Tons of stress and migranes the past few days. I might be on the > verge >of going Histo if I'm not careful. Is it Friday yet? >Claire > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Pier >Sent: Thu 3/12/2009 1:21 PM >To: godsgalnow@aol.com; HornHV@archildrens.org; anne.lewin@bms.com; >billodonnell@catholichealth.net; histonet@lists.utsouthwestern.edu; >jlhowery@yrmc.org >Subject: RE: [Histonet] Dako > > > >I've had great luck with Biocare as well. They've been my primary supplier >for detection kits and ancillaries since 2003. I'm probably going to check >them out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 >mL. > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Mar 16 08:04:45 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Mar 16 08:04:56 2009 Subject: [Histonet] Disposal of Formaldehyde In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04C2F537@mailsvr.MARSHMED.local> Message-ID: <7666661AC38148338FE71DADCE3B2497@lurie.northwestern.edu> We place it in 5 gallon containers and the office of research safety comes and picks it up and it goes to a waste hauler form there. They pick up alcohol as well as xylene. If we had the space for a recycler...... Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Friday, March 13, 2009 9:27 AM To: Jessica Piche; histonet Subject: RE: [Histonet] Disposal of Formaldehyde First ... even if the local authorities allow this ... it doesn't make "Green" sense to do it, especially when there are other very workable alternatives. The path we chose was to purchase a very simple gravity feed recycler produced and sold by Creative Waste Solutions (888) 795-8300. Check them out. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 7:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde ? Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Mar 16 08:14:23 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Mar 16 08:14:26 2009 Subject: [Histonet] decolorizing MMA Message-ID: Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From SwainFrancesL <@t> uams.edu Mon Mar 16 08:20:34 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Mon Mar 16 08:21:07 2009 Subject: [Histonet] RE: decolorizing MMA In-Reply-To: References: Message-ID: What do you mean by decolorize? Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, March 16, 2009 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decolorizing MMA Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From pruegg <@t> ihctech.net Mon Mar 16 08:27:41 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Mar 16 08:27:46 2009 Subject: [Histonet] decolorizing MMA In-Reply-To: References: Message-ID: <286489D86B44436D847E9779AD42F503@prueggihctechlt> Do you mean deplasticize, as in remove the resin? If that is what you mean, yes, you can remove mma with xylene, you cannot remove GMA. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, March 16, 2009 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decolorizing MMA Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Mon Mar 16 08:34:24 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Mon Mar 16 08:35:41 2009 Subject: [Histonet] decolorizing MMA In-Reply-To: <286489D86B44436D847E9779AD42F503@prueggihctechlt> References: <286489D86B44436D847E9779AD42F503@prueggihctechlt> Message-ID: If you want to remove the MMA you can also use 2-Methoxyethyl Acetate or Acetone. If you want to decolorize like a hematoxylin, yes you can do that also. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, March 16, 2009 8:28 AM To: 'Molinari, Betsy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decolorizing MMA Do you mean deplasticize, as in remove the resin? If that is what you mean, yes, you can remove mma with xylene, you cannot remove GMA. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, March 16, 2009 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decolorizing MMA Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From godsgalnow <@t> aol.com Mon Mar 16 09:55:45 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 16 09:56:02 2009 Subject: [Histonet] grossing Message-ID: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> I hate to beat a dead horse.....but how do you handle non-certified grossing staff.? They all meet the CLIA guidelines, but are they allowed to gross in the evening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that gross....according to the CAP guidelines? Thanks, Roxanne From histonetalias <@t> gmail.com Mon Mar 16 10:13:16 2009 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Mon Mar 16 10:13:20 2009 Subject: [Histonet] grossing In-Reply-To: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> References: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> Message-ID: <4b6c85510903160813h63bff210xc8c4fd5c30f9328d@mail.gmail.com> Define presence? If they are able to have access a technical supervisor or a Pathologist if they have a question then this may just cover it. We have a Pathologist "on call" that can be in within minutes if there are any questions. On Mon, Mar 16, 2009 at 10:55 AM, wrote: > I hate to beat a dead horse.....but how do you handle non-certified > grossing staff.? They all meet the CLIA guidelines, but are they allowed to > gross in the evening without the presence of a technical supervisor? > > And how do you handle salaries for those that process and those that > gross....according to the CAP guidelines? > > > Thanks, > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From MElliott <@t> mrl.ubc.ca Mon Mar 16 10:14:11 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Mon Mar 16 10:14:57 2009 Subject: [Histonet] Antibody advice In-Reply-To: <5A1DCB94.428@mail.mrl.ubc.ca> References: <5A1DCB94.428@mail.mrl.ubc.ca> Message-ID: <49BE0A53.11C6.00D6.0@mrl.ubc.ca> Good morning everyone We are starting a rather large project and I would like to get some input as to the best version of antibodies to the following antigens-what are people using for the following and where do you get them from. This will be either frozen sections or formalin fixed paraffin embedded. CCR7 S100A8 CXCL13 SMAD6 Thy-1/CD90 HSP47 Prolyl-4-hydroxylase Thanks for your input. It is much appreciated Mark in cloudy damp Vancouver BC ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From rjbuesa <@t> yahoo.com Mon Mar 16 10:24:00 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 16 10:24:03 2009 Subject: [Histonet] grossing In-Reply-To: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> Message-ID: <782161.16926.qm@web65706.mail.ac4.yahoo.com> >From my point of view, shared by all AP and many managers, the answer is NO, they cannot, and should not. Histology samples are too precious to be handled by somebody without the proper training. Ren? J. --- On Mon, 3/16/09, godsgalnow@aol.com wrote: From: godsgalnow@aol.com Subject: [Histonet] grossing To: histonet@lists.utsouthwestern.edu Date: Monday, March 16, 2009, 10:55 AM I hate to beat a dead horse.....but how do you handle non-certified grossing staff.? They all meet the CLIA guidelines, but are they allowed to gross in the evening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that gross....according to the CAP guidelines? Thanks, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Mon Mar 16 10:35:11 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 16 10:36:00 2009 Subject: [Histonet] grossing In-Reply-To: <782161.16926.qm@web65706.mail.ac4.yahoo.com> Message-ID: <8CB7463EDE66CD0-84C-E12@webmail-dd09.sysops.aol.com> Ren?, my friend.... This is my opinion as well and I have stated so, but is there any documentation? Roxanne -----Original Message----- From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; godsgalnow@aol.com Sent: Mon, 16 Mar 2009 11:24 am Subject: Re: [Histonet] grossing >From my point of view, shared by all AP and many managers, the answer is NO, they cannot, and should not. Histology samples are too precious to be handled by somebody without the proper training. Ren? J. --- On Mon, 3/16/09, godsgalnow@aol.com wrote: From: godsgalnow@aol.com Subject: [Histonet] grossing To: histonet@lists.utsouthwestern.edu Date: Monday, March 16, 2009, 10:55 AM I hate to beat a dead horse.....but how do you handle non-certified grossing taff.? They all meet the CLIA guidelines, but are they allowed to gross in the vening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that ross....according to the CAP guidelines? hanks, oxanne ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Mon Mar 16 11:03:21 2009 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Mon Mar 16 11:03:25 2009 Subject: [Histonet] TweakR Message-ID: <49BE7849.9000400@tufts.edu> Hi all, I'm trying to use Tweak R (Fn14) on mouse lung. I've got a rabbit polyclonal from abcam that supposedly is validated for IHC-paraffin - have tried it with and without HIER (citrate) and with and without detergents - was worried that as its a transmembrane protein maybe I was washing it away. Has anybody done this on paraffins? If so, can you share a protocol? Many thanks - Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu From godsgalnow <@t> aol.com Mon Mar 16 11:11:17 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 16 11:11:44 2009 Subject: [Histonet] grossing In-Reply-To: <4b6c85510903160813h63bff210xc8c4fd5c30f9328d@mail.gmail.com> References: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> <4b6c85510903160813h63bff210xc8c4fd5c30f9328d@mail.gmail.com> Message-ID: <8CB7468F878512F-84C-107F@webmail-dd09.sysops.aol.com> Processing, according to CAP? : "tissue examination limited to description, inking and cutting of the specimen, and submission og the entire specimen to histology.? Tissue processing can be performed accodring to standardized protocols.? Processing is generally limited to small specimens and does not require knowledge of anatomy." This is right from the CAP checklist (ANP.11600) Roxanne -----Original Message----- From: Histonet Alias To: godsgalnow@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Mon, 16 Mar 2009 11:13 am Subject: Re: [Histonet] grossing Define presence? If they are able to have access a technical supervisor or a Pathologist if they have a question then this may just cover it. We have a Pathologist "on call" that can be in within minutes if there are any questions.? On Mon, Mar 16, 2009 at 10:55 AM, wrote: I hate to beat a dead horse.....but how do you handle non-certified grossing staff.? They all meet the CLIA guidelines, but are they allowed to gross in the evening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that gross.....according to the CAP guidelines? Thanks, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From HornHV <@t> archildrens.org Mon Mar 16 11:41:00 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Mar 16 11:41:05 2009 Subject: [Histonet] paul moylan from dako Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830BD@EMAIL.archildrens.org> Paul Moylan from Dako please contact me. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From JBower <@t> hei.org Mon Mar 16 11:42:00 2009 From: JBower <@t> hei.org (Bower, Jennifer) Date: Mon Mar 16 11:42:09 2009 Subject: [Histonet] IHC stainer In-Reply-To: <854584B5780149D884938E2198F1622B@prueggihctechlt> References: <1275753817.60818181236995378105.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> <854584B5780149D884938E2198F1622B@prueggihctechlt> Message-ID: <87449E4A2B01DA47B29424CE5D6E0F830BB2C6F1@hi0sml1.hei.org> I am in a research lab and we are currently testing automated IHC machines. A couple months ago we had the Bond Max from Leica. The machine gave me nothing but problems. They said it was because it was a "demo" unit and the real one would not do that (who knows). The interesting thing, though, was the quote I received from them. Yes, if you pay extra you can "open" the system so that you can use any type of reagent. What they don't tell you is you have to ALSO pay about $1000 for EACH detection system you want to use. For research, that is a little crazy, I could use a different detection system every day. Currently we have the Dako Autostainer. It is completely open and I have had no problems with it. I don't know what was meant by Dako going with a more "closed" system, the machine we are currently testing is very open. I like it. Jennifer Bower -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, March 15, 2009 10:05 AM To: 'Pamela Marcum'; 'Greg Dobbin' Cc: histonet@lists.utsouthwestern.edu; sweaver@tvmdl.tamu.edu; 'Ronald Houston' Subject: RE: [Histonet] IHC stainer I have for years worked with animal and human IHC and would have said in the past that the Dako open system autostainer was the way to go with the most flexibility, I still have the old one and use it all the time because you can absolutely use what ever reagent you want and there are way cheaper ways to go than buying these from Dako, Ventana, Leica, or BioCare, or the others. Now that Dako has gone with the more closed system like Ventana I cannot recommend them for open use anymore, a big mistake by Dako in my opinion. The Leica Bond instrument may be an option. They have a research software version that allows you to use open containers and fill them with whose ever reagents you want, BioCare also has an open system new on the market. Sometimes I wonder if in research IHC if it might not be a lot cheaper and we would have better control and understand more about what we are doing if we went back to manual IHC. For the Vet. Lab wanting to start their own IHC work with not a lot of volume and untrained techs, I would definitely want my techs to learn by doing manual staining before I put some instrument in there that will teach them more about how to use the computer software than anything about IHC, but that is just the opinion of an old school gal who sees that there are a lot of people doing critical IHC work who do not understand what they are doing. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Friday, March 13, 2009 7:50 PM To: Greg Dobbin Cc: histonet@lists.utsouthwestern.edu; sweaver@tvmdl.tamu.edu; Ronald Houston Subject: Re: [Histonet] IHC stainer Just be sure it is an open system that will allow you to use any primary antibody you need and secondary antibody not just a secondary kit the company provides. You will need to confirm that if you are using a chicken primary for instance, you can get and use a secondary link that will work with the chicken primary to get your staining reaction. Be very careful that it allows you flexibilty and is not designed for clinical standard usage with no ability to truly allow you the range required for animal work. I work with animal and it is hard to find everyhting you need and not all "standard" antibodies and systems will work across species. Good Luck, Pam marcum ----- Original Message ----- From: "Greg Dobbin" To: histonet@lists.utsouthwestern.edu, "Ronald Houston" , sweaver@tvmdl.tamu.edu Sent: Friday, March 13, 2009 8:57:48 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] IHC stainer I guess I would have to echo that as well! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Houston, Ronald" 3/13/2009 4:45:14 PM >>> Can't speak highly enough of the BondMax form Leica Microsystems. If cost is an issue and you're looking at what to avoid, IMHO avoid anything Ventana Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver Sent: Friday, March 13, 2009 3:17 PM To: histonet post Subject: [Histonet] IHC stainer I am in a veterinary diagnostic lab. In the past we have had very few requests for IHC and have always sent slides out to another lab to perform IHC as needed. It is time for us to start doing our own and join the modern age. We have several certified technicians, but none have experience with IHC and we typically have a relatively high turnover rate. Therefore, I am hoping to be able to buy an automated stainer. In the past most people on the list seemed to be very happy with the Dako autostainer, but this past week has brought so many bad remarks about Dako's service that I am reconsidering. We probably will not need a high capacity autostainer, but I would like walk-away capability with an easy to use system. It will need to accept other companies reagents, since veterinary infectious disease antibodies aren't often sold by the major companies. Also, cost is an issue and I would like to be able to bargain shop for reagents through other companies. Does anyone have any recommendations, or warnings as to what to avoid? In a related issue, where do other animal tissue people get their antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or FIP? Thanks for the advice! Stephanie Weaver Texas Veterinary Medical Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonetalias <@t> gmail.com Mon Mar 16 12:40:23 2009 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Mon Mar 16 12:40:27 2009 Subject: [Histonet] grossing In-Reply-To: <8CB7468F878512F-84C-107F@webmail-dd09.sysops.aol.com> References: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> <4b6c85510903160813h63bff210xc8c4fd5c30f9328d@mail.gmail.com> <8CB7468F878512F-84C-107F@webmail-dd09.sysops.aol.com> Message-ID: <4b6c85510903161040g1d2af0ddx65de860a6fb974b9@mail.gmail.com> This is another famous gray area that CAP has left us. Are your techs grossing or processing? We split up specimens between processing and grossing. A PA will to the grossing and a technician will do the processing. We have a PA or Path on call for any questions in the evening. If the tech is unsure then the PA or Path comes in or leaves it until the next day. ANP.11600 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. On Mon, Mar 16, 2009 at 12:11 PM, wrote: > Processing, according to CAP : > > "tissue examination limited to description, inking and cutting of the > specimen, and submission og the entire specimen to histology. Tissue > processing can be performed accodring to standardized protocols. Processing > is generally limited to small specimens and does not require knowledge of > anatomy." > > This is right from the CAP checklist (ANP.11600) > > Roxanne > > > > > > -----Original Message----- > From: Histonet Alias > To: godsgalnow@aol.com > Cc: histonet@lists.utsouthwestern.edu > Sent: Mon, 16 Mar 2009 11:13 am > Subject: Re: [Histonet] grossing > > Define presence? If they are able to have access a technical supervisor or > a Pathologist if they have a question then this may just cover it. We have a > Pathologist "on call" that can be in within minutes if there are any > questions. > > On Mon, Mar 16, 2009 at 10:55 AM, wrote: > >> I hate to beat a dead horse.....but how do you handle non-certified >> grossing staff.? They all meet the CLIA guidelines, but are they allowed to >> gross in the evening without the presence of a technical supervisor? >> >> And how do you handle salaries for those that process and those that >> gross.....according to the CAP guidelines? >> >> >> Thanks, >> Roxanne >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Al Ias HT(ASCP) > Histology Manager > Pathology Laboratory > United States > > ------------------------------ > *A Good Credit Score is 700 or Above. See yours in just 2 easy steps! > * > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From Margaret.Perry <@t> sdstate.edu Mon Mar 16 12:49:34 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Mar 16 12:49:41 2009 Subject: [Histonet] Looking for Patsy Ruegg's e-mail address In-Reply-To: <20090316164232.C4700920728@barracuda.sdstate.edu> References: <20090316164232.C4700920728@barracuda.sdstate.edu> Message-ID: Patsy I have a couple of questions for you but have the wrong address. Margaret Perry From alyssa <@t> alliedsearchpartners.com Mon Mar 16 13:35:25 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Mar 16 13:35:30 2009 Subject: [Histonet] Histotechnologist Position in Boston-Direct Hire Message-ID: Hello Histonetters! I hope everyone had a great weekend. I wanted to follow up in reference to a full time, direct hire position for a histotechnologist in Boston. My company offers a referral bonus up to $1000 paid to you if your referral is hired. Forward this to anyone you feel fit. If you are interested please respond with a current copy of your resume attached as a Microsoft word document, and a contact number with the best time to reach you. You can call me anytime, at 770.621.2639 ext. 4 or send me an email at alyssa@alliedsearchpartners.com for further information. ** *Please review the job description:* Must have ASCP certification, with at least 2 year experience. Full time regular employment, Day Shift, 6am-2:30pm. *Benefits: * My client offers an attractive benefits package for full time employment, including medical/dental insurance, PTO, 401K, and much more!!!!!! Salary is the highly competitive based on experience. -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From ratliffjack <@t> hotmail.com Mon Mar 16 13:47:03 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Mar 16 13:47:09 2009 Subject: [Histonet] decolorizing MMA In-Reply-To: References: Message-ID: Are you talking about thin microtomed sections or thicker "ground" sections???? Thin MMA embedded sections can be deplastified similar to the removal of paraffin embedded specimens, but thicker "ground" MMA sections cannot be deplastified. However, maybe you are making reference to re-staining a slide like in a paraffin embedded H&E where you can reverse the staining order and stain again???? I have not tried it on thin sections and suspect that it would depend upon the stain used. Also, you might have a problem with the section becoming loose or falling off the slide. As for the thicker "ground" sections, you should be able to re-polish the slides (depending upon true section thickness) and then stain again. Keep in mind that when staining these thicker MMA sections, most (if not all) typical stains only surface stain the section in the first place due to the size of the staining molecules and their ability to penetrate the resin w/ and/or w/o acid etching techniques. Jack Ratliff > Date: Mon, 16 Mar 2009 08:14:23 -0500 > From: BMolinari@heart.thi.tmc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] decolorizing MMA > > Good morning, > > Is it possible to decolorize MMA slides? > > Thanks, > > Betsy > > > > Betsy Molinari HT(ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave > > MC1-283 > > Houston,TX 77030-2607 > > 832-355-6524 > > 832-355-6812 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Mon Mar 16 14:33:01 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 16 14:36:02 2009 Subject: [Histonet] grossing In-Reply-To: <4b6c85510903161040g1d2af0ddx65de860a6fb974b9@mail.gmail.com> References: <8CB745E6B757B34-84C-B66@webmail-dd09.sysops.aol.com> <4b6c85510903160813h63bff210xc8c4fd5c30f9328d@mail.gmail.com> <8CB7468F878512F-84C-107F@webmail-dd09.sysops.aol.com> <4b6c85510903161040g1d2af0ddx65de860a6fb974b9@mail.gmail.com> Message-ID: <8CB74852798DF86-914-C7@WEBMAIL-MY07.sysops.aol.com> Actually, as long as they meet the standards for High Complexity testing they can gross.......according to CAP you have to have no knowledge of anatomy to process..... Roxanne -----Original Message----- From: Histonet Alias To: godsgalnow@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Mon, 16 Mar 2009 1:40 pm Subject: Re: [Histonet] grossing This is another famous gray area that CAP has left us. Are your techs grossing or processing? We split up specimens between processing and grossing. A PA will to the grossing and a technician will do the processing. We have a PA or Path on call for any questions in the evening. If the tech is unsure then the PA or Path comes in or leaves it until the next day.? ANP.11600 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. On Mon, Mar 16, 2009 at 12:11 PM, wrote: Processing, according to CAP? : "tissue examination limited to description, inking and cutting of the specimen, and submission og the entire specimen to histology.? Tissue processing can be performed accodring to standardized protocols.? Processing is generally limited to small specimens and does not require knowledge of anatomy." This is right from the CAP checklist (ANP.11600) Roxanne -----Original Message----- From: Histonet Alias To: godsgalnow@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Mon, 16 Mar 2009 11:13 am Subject: Re: [Histonet] grossing Define presence? If they are able to have access a technical supervisor or a Pathologist if they have a question then this may just cover it. We have a Pathologist "on call" that can be in within minutes if there are any questions.? On Mon, Mar 16, 2009 at 10:55 AM, wrote: I hate to beat a dead horse.....but how do you handle non-certified grossing staff.? They all meet the CLIA guidelines, but are they allowed to gross in the evening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that gross......according to the CAP guidelines? Thanks, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States A Good Credit Score is 700 or Above. See yours in just 2 easy steps! -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From dcojita <@t> tampabay.rr.com Mon Mar 16 15:35:23 2009 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Mon Mar 16 15:35:30 2009 Subject: [Histonet] grossing In-Reply-To: <8CB74852798DF86-914-C7@WEBMAIL-MY07.sysops.aol.com> Message-ID: According to CLIA, the employee who grosses must also possess a state license when working in a state that requires licensure, such as Florida. Keep in mind that CAP rules do not apply for those laboratories that are not CAP accredited. Exert from CLIA - "In order to qualify as high complexity testing personnel under Sec. 493.1489(b)(3), the individual must have met or could have met the following qualifications for technologist as they were in effect on or before February 28, 1992. Each technologist must-- (a) Possess a current license as a laboratory technologist issued by the State, if such licensing exists;" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, March 16, 2009 3:33 PM To: histonetalias@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing Actually, as long as they meet the standards for High Complexity testing they can gross.......according to CAP you have to have no knowledge of anatomy to process..... Roxanne -----Original Message----- From: Histonet Alias To: godsgalnow@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Mon, 16 Mar 2009 1:40 pm Subject: Re: [Histonet] grossing This is another famous gray area that CAP has left us. Are your techs grossing or processing? We split up specimens between processing and grossing. A PA will to the grossing and a technician will do the processing. We have a PA or Path on call for any questions in the evening. If the tech is unsure then the PA or Path comes in or leaves it until the next day.? ANP.11600 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. On Mon, Mar 16, 2009 at 12:11 PM, wrote: Processing, according to CAP? : "tissue examination limited to description, inking and cutting of the specimen, and submission og the entire specimen to histology.? Tissue processing can be performed accodring to standardized protocols.? Processing is generally limited to small specimens and does not require knowledge of anatomy." This is right from the CAP checklist (ANP.11600) Roxanne -----Original Message----- From: Histonet Alias To: godsgalnow@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Mon, 16 Mar 2009 11:13 am Subject: Re: [Histonet] grossing Define presence? If they are able to have access a technical supervisor or a Pathologist if they have a question then this may just cover it. We have a Pathologist "on call" that can be in within minutes if there are any questions.? On Mon, Mar 16, 2009 at 10:55 AM, wrote: I hate to beat a dead horse.....but how do you handle non-certified grossing staff.? They all meet the CLIA guidelines, but are they allowed to gross in the evening without the presence of a technical supervisor? And how do you handle salaries for those that process and those that gross......according to the CAP guidelines? Thanks, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States A Good Credit Score is 700 or Above. See yours in just 2 easy steps! -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Mon Mar 16 15:55:11 2009 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Mar 16 15:55:15 2009 Subject: [Histonet] BrdU immunostain on rat brain section Message-ID: <561028.63019.qm@web38003.mail.mud.yahoo.com> Hello, I was asked to do BrdU immunostain on rat brain tissue. Does anyone have experience of it? 1.Do you prefer frozen or paraffin? 2.Which antibody you recommend? 3.?If??35um frozen is requied, what thickness you will use if you want go for paraffin? ? ? Thanks, Amy From DKnutson <@t> primecare.org Mon Mar 16 16:26:09 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Mon Mar 16 16:25:58 2009 Subject: [Histonet] Fix Sediment Method Message-ID: <4F0B7161A6CD524FAD8017D52E1553400A7687F4@exchangent> Fellow Histonetters - I am looking for feedback on the alternative methods to prepare Non GYN cellblocks. We currently use the Fix Sediment Method where you spin the fluid to achieve a button, pour off the supernatant, and pour formalin onto the button to harden it. What do others do? Does anyone use the Bacterial Agar Method? Thank you in advance for any information and advice you can send me. Deanne Knutson From tifei <@t> foxmail.com Mon Mar 16 21:10:39 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Mon Mar 16 21:11:30 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gQnJkVSBpbW11bm9zdGFpbiBvbiByYXQgYnJhaW4gc2VjdGlvbg==?= References: <561028.63019.qm@web38003.mail.mud.yahoo.com> Message-ID: <200903171010338132746@foxmail.com> hi, we worked a lot on this. I prefer to use 40 um frozen sections. Abcam anti-BrdU (rat sourced) is the best ever I know. It does not have high background with rat IgG. Some other mouse-/sheep- sourced BrdU are available. 2009-03-17 TF ???? Amy Lee ????? 2009-03-17 05:02:28 ???? histonet ??? ??? [Histonet] BrdU immunostain on rat brain section Hello, I was asked to do BrdU immunostain on rat brain tissue. Does anyone have experience of it? 1.Do you prefer frozen or paraffin? 2.Which antibody you recommend? 3.?f?35um frozen is requied, what thickness you will use if you want go for paraffin? ? ? Thanks, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SARAH.REEVES <@t> ekht.nhs.uk Tue Mar 17 03:58:25 2009 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Tue Mar 17 03:59:02 2009 Subject: [Histonet] Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues. Message-ID: <49BF6631020000B5000042F0@ekhgwia.ekht.nhs.uk> Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues. ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From SARAH.REEVES <@t> ekht.nhs.uk Tue Mar 17 07:18:14 2009 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Tue Mar 17 07:18:40 2009 Subject: [Histonet] H+E Skin Staining Message-ID: <49BF9506020000B50000431C@ekhgwia.ekht.nhs.uk> I am interested in the reasons behind the lack of chromatin detail in skin sections. Although this is not always a problem it is a concern. Could the problem be the processing? or are there other factors related to skin? ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From joost.bruijntjes <@t> tno.nl Tue Mar 17 07:47:16 2009 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Tue Mar 17 07:47:22 2009 Subject: [Histonet] (no subject) Message-ID: <8865601DD17A554CB489C17FFD8A51B20230764B@MAIL04.tsn.tno.nl> Hi histonetters Is anyone of you working with a cd86 on mouse tissues, preferable on FFPE tissues? Thanks Joost Bruijntjes Tox & Applied Pharmacol. Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From anh2006 <@t> med.cornell.edu Tue Mar 17 08:57:07 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Mar 17 08:57:10 2009 Subject: [Histonet] DyLight fluorophores Message-ID: Any thoughts on the new DyLight series of fluorophores from Jackson ImmunoResearch? http://www.jacksonimmuno.com/technical/DyLight.asp -- From kmerriam2003 <@t> yahoo.com Tue Mar 17 09:20:23 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Mar 17 09:20:28 2009 Subject: [Histonet] IHC after Sudan Black Message-ID: <627990.73611.qm@web50311.mail.re2.yahoo.com> Hi, I am asking this for a colleague of?mine - can you do IHC after a Sudan Black stain has been performed on a slide?? Can Sudan Black be decolorized without interfering with antigenicity? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From histonet.nospam <@t> vneubert.com Tue Mar 17 09:59:00 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Mar 17 09:59:04 2009 Subject: [Histonet] Microm HM325 users Message-ID: <202650f2ff26da1d97e8b229b86ad6ea@vneubert.com> Hello Histonetters! Is anyone using a Microm HM325 microtome? Please contact me if you want to share some tricks and useful hints, concerning sectioning to me :) I don't have a question in particular, just want to see if anyone is doing sth like cooling the blade or so. Bye, V. Neubert From MLashus <@t> pathgroup.com Tue Mar 17 10:27:32 2009 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Tue Mar 17 10:27:38 2009 Subject: [Histonet] Histology Opportunity Message-ID: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> We have an opening at our Chattanooga lab for a third shift Histo tech. The hours are 11:30 pm to 8:00am. The candidate we are searching for will be eventually groomed for a supervisory position. We offer an excellent benefit package along with a very friendly working environment. If you are interested please contact me either by phone or you may fax your resume to the number listed below. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From billodonnell <@t> catholichealth.net Tue Mar 17 10:36:04 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Mar 17 10:36:15 2009 Subject: [Histonet] DAB In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> Message-ID: Greetings I recall that years ago Biomeda had a product called Stable DAB. It was a ready-to-use product that was stored in the freezer. Does anyone know if it is still being produced and whom I should contact? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From SwainFrancesL <@t> uams.edu Tue Mar 17 10:43:25 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Tue Mar 17 10:44:09 2009 Subject: [Histonet] RE: DAB In-Reply-To: References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> Message-ID: There was a discussion about the Stable DAB on the histonet a few weeks ago. I would suggest you search the archieves of the histonet. The company that has this product was listed at that time. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, March 17, 2009 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB Greetings I recall that years ago Biomeda had a product called Stable DAB. It was a ready-to-use product that was stored in the freezer. Does anyone know if it is still being produced and whom I should contact? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From anitaibsc <@t> aol.com Tue Mar 17 10:59:28 2009 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Tue Mar 17 11:00:17 2009 Subject: [Histonet] DAB In-Reply-To: References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> Message-ID: <8CB75307C298ED7-8A8-5EA@webmail-me11.sysops.aol.com> Greetings! The research scientist of Biomeda has founded ImmunoBioScience Corp., therefore offering a direct replacement of Biomeda products. Please visit www.ImmunoBioScience.com Thank you. Best regards, Anita -----Original Message----- From: O'Donnell, Bill To: histonet@lists.utsouthwestern.edu Sent: Tue, 17 Mar 2009 8:36 am Subject: [Histonet] DAB Greetings I recall that years ago Biomeda had a product called Stable DAB. It was a ready-to-use product that was stored in the freezer. Does anyone know if it is still being produced and whom I should contact? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gp62 <@t> georgetown.edu Tue Mar 17 11:18:10 2009 From: gp62 <@t> georgetown.edu (Guillermo Palchik) Date: Tue Mar 17 11:18:34 2009 Subject: [Histonet] curling on cryostat brain sections and other issues In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> Message-ID: <0C91369F-F2EB-4905-BD86-504569CECAA4@georgetown.edu> Hi there, I am having problems with my brain sections (15 um - flash-frozen) curling up once I remove the antiroll plate on my cryostat... does anyone know how i can prevent this? Also - does anybody have a "quick guide" on what to improve while cutting, based on the damage seen on the brain slice: for example I heard that if there are vertical lines along the slice, it might mean a nick in the blade, that cracking of the tissue might be cutting with the blade too perpendicular, and so on... i am looking for a general troubleshooting guide for cryostat cutting... thanks Gil -- There are people who fight one day and are good... There are those who fight one year and are better... There are people who fight many years and are very good... But there are those who fight their entire lives: they are indispensable... Bertolt Brecht (1898-1956) How can it be that mathematics, being after all a product of human thought which is independent of experience, is so admirably appropriate to the objects of reality? Is human reason, then, without experience, merely by taking thought, able to fathom the properties of real things? Albert Einstein (1879-1955) From carrolpb <@t> umdnj.edu Tue Mar 17 11:34:53 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Mar 17 11:35:17 2009 Subject: [Histonet] curling on cryostat brain sections and other issues In-Reply-To: <0C91369F-F2EB-4905-BD86-504569CECAA4@georgetown.edu> References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> <0C91369F-F2EB-4905-BD86-504569CECAA4@georgetown.edu> Message-ID: <49BFD12D.1080609@umdnj.edu> > I am having problems with my brain sections (15 um - flash-frozen) curling up once I remove the antiroll plate on my cryostat... I cut many of the same on a regular basis, and I find that letting them sit for a a few moments (~10 seconds) helps keep them from curling, at least long enough to pick them up with the slide! > From carrolpb <@t> umdnj.edu Tue Mar 17 11:41:27 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Mar 17 11:45:09 2009 Subject: [Histonet] curling on cryostat brain sections and other issues In-Reply-To: <49BFD12D.1080609@umdnj.edu> References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup.com> <0C91369F-F2EB-4905-BD86-504569CECAA4@georgetown.edu> <49BFD12D.1080609@umdnj.edu> Message-ID: <49BFD2B7.3050403@umdnj.edu> er, sit for a few moments under the anti-curling plate, that is... Peter Carroll wrote: > > I am having problems with my brain sections (15 um - flash-frozen) > curling up once I remove the antiroll plate on my cryostat... > > I cut many of the same on a regular basis, and I find that letting > them sit for a a few moments (~10 seconds) helps keep them from > curling, at least long enough to pick them up with the slide! > > >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From alyssa <@t> alliedsearchpartners.com Tue Mar 17 12:08:26 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Mar 17 12:08:31 2009 Subject: [Histonet] Full Time Direct Hire Positions In TX & Las Vegas Message-ID: Hello All, Just a quick Hello to let you all know that I am looking for talented histotech's for *Corpus Christi, TX and Las Vegas, NV*. These are full time, Permanent Positions. Contact me @ alyssa@alliedsearchpartners.com for the details if you are interested. The positions offer full benefits, relocation assistance, and top pay among just a few things! Also, do not forgot about our referral $1000 referral bonus, so please spread the word and have your referrals contact me today! Thank you!!!! -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From leiker <@t> buffalo.edu Tue Mar 17 12:04:58 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 17 12:13:20 2009 Subject: [Histonet] curling on cryostat brain sections and other issues In-Reply-To: <0C91369F-F2EB-4905-BD86-504569CECAA4@georgetown.edu> References: <197CD0B02A81F94994A285C59C8AE05C04748730FD@pgnexchange.pathgroup .com> <0C91369F-F2EB-4905-BD86-504569CECAA4@georgetown.edu> Message-ID: <0896714B4570E3968D6F8F60@bchwxp2702.ad.med.buffalo.edu> I make 8um-thick sections of various tissues and sometimes they do curl. I do not use an anti-roll plate. So what I do while making the section is to cut just enough of the section so that it is attached by a very small amount to the top of the block, (so that most of the block has gone down below the blade and won't interfere when you pick up the section with your slide). The free end of the section will still curl; however, you only have this end to deal with since the other is fixed! I use my brushes to flatten out this edge as much as possible, then quickly place the slide on it before it starts curling up again. It usually will curl up rather slowly after having its edges "brushed", giving me plenty of time. Sometimes it stays flat and doesn't curl at all. But remember this is 8-um sections, not 15um. Merced --On Tuesday, March 17, 2009 12:18 PM -0400 Guillermo Palchik wrote: > Hi there, > I am having problems with my brain sections (15 um - flash-frozen) > curling up once I remove the antiroll plate on my cryostat... does > anyone know how i can prevent this? > Also - does anybody have a "quick guide" on what to improve while > cutting, based on the damage seen on the brain slice: for example I > heard that if there are vertical lines along the slice, it might mean a > nick in the blade, that cracking of the tissue might be cutting with the > blade too perpendicular, and so on... i am looking for a general > troubleshooting guide for cryostat cutting... > thanks > Gil > > -- > There are people who fight one day and are good... > There are those who fight one year and are better... > There are people who fight many years and are very good... > But there are those who fight their entire lives: they are > indispensable... > > Bertolt Brecht (1898-1956) > > > How can it be that mathematics, being after all a product > of human thought which is independent of experience, is so > admirably appropriate to the objects of reality? > Is human reason, then, without experience, merely by taking thought, > able to fathom the properties of real things? > > Albert Einstein (1879-1955) > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Rick.Garnhart <@t> memorialhealthsystem.com Tue Mar 17 12:16:22 2009 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Tue Mar 17 12:18:33 2009 Subject: [Histonet] Grossing In-Reply-To: Message-ID: CAP is OK with their guidelines ( BUTTTTTTTTT is CLIA on line with the Processing (Low Complexity?) vs Grossing (High Complexity?). If CLIA is not on board with processing (dumb naming) being low complexity and grossing being higher complexity your lab may be in violation of CLIA Guidelines. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From petepath <@t> yahoo.com Tue Mar 17 12:57:20 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Mar 17 12:57:25 2009 Subject: [Histonet] re:curling on cryostat brain sections and other issues Message-ID: <345178.29880.qm@web45105.mail.sp1.yahoo.com> At the temperture just after what I call the "crumple stage", when the tissue and medium are stiff enough to form under the blade in a complete section, the section will lie flat with out shattering.?If your brush is clean you can cut multiple in a row like ribbons. This is in the - 16 to -18C range. As the temperature gets colder the sections will curl, slightly at first and then more and more and accompanied by increasing shattering. With brain tissue, which is quite watery shattering will occur at relativly higher temperatures than less watery tissues. If you are using very cold blocks you can warm it back to this crumple stage with the thumb and cut a few sections until it recools on its? own to that temperature where it cuts perfectly. If you would like to learn more about frozen section technique visit the free tutorial on my web site at this link: http://www.pathologyinnovations.com/frozen_section_technique.htm ? Stephen Peters M.D.? 201 847 0052 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From pbaldwin <@t> theadvisoryboardprogram.com Tue Mar 17 13:16:43 2009 From: pbaldwin <@t> theadvisoryboardprogram.com (Peter Baldwin) Date: Tue Mar 17 13:17:07 2009 Subject: [Histonet] Disposal of Formaldehyde Message-ID: Ruth is on target with her answer.? EPA regulates all "solid" (which includes liquids) hazardous waste produced by healthcare facilities and others, among which formaldehyde is classified as a "listed" chemical (code U122).? If a chemical is not "listed" (or specifically identified), it still is classified as hazardous if it is ignitable, corrosive, reactive or toxic. All hazardous wastes must be handled (including monitoring, storage, and disposal) in accordance with EPA regs, which specifically state that hazardous wastes generated in the (healthcare) laboratory "cannot be disposed into drains."? So even though there may be additional local governmental regulations with which each facility must comply, they cannot supersede the EPA regs. The EPA Website is full of dispersed hazardous waste information, but a good starting point is: http://www.hercenter.org Peter Peter G. Baldwin Director of Sales, Marketing & Business Development pbaldwin@theadvisoryboardprogram.com Micron Environmental Industries, Inc. 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax www.MicronEnvironmental.com From tammy <@t> surgicalpathlabs.com Tue Mar 17 13:25:03 2009 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Tue Mar 17 13:36:52 2009 Subject: [Histonet] Florida Histo Openings Message-ID: Good morning. We are a Pathology Lab in Pinellas Park, Florida. We're looking to add to our team! Candidates should be an HTL or HT (ASCP) or equivalent, have a valid driver's license and proof of insurability. Primary responsibilities include on site frozen sections including mobile laboratory units. We offer a full benefits package with a great working environment. View our Company Info and job details at www.surgicalpathlabs.com. Thanks. Tammy R. de Leon Surgical Pathology Laboratories Human Resources Officer 8455 66th Street N Pinellas Park, Fl 33781 Office Phone - 727-209-1215 Fax - 727-545-1644 From galinadeyneko <@t> yahoo.com Tue Mar 17 14:40:39 2009 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Mar 17 14:40:45 2009 Subject: [Histonet] succinate dehydrogenase protocol Message-ID: <166529.77764.qm@web33108.mail.mud.yahoo.com> Dear Colleagues I should detect /stain Succinate dehydrogenase in mouse muscle non-fixed?frozen sections. Could someone?please share detailed protocol and sources of the reagents. Does it exist any?? kit for detection? I performed some on-line search but did not find a sufficient information. Thank you in advance. Galina Deyneko Novartis, Cambridge, MA 617-871-7613. From gu.lang <@t> gmx.at Tue Mar 17 15:21:57 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 17 15:22:05 2009 Subject: [Histonet] euchromatin and heterochromatin Message-ID: Hi dear histonetters, I have a question about chromatin and its stainability. Heterochromatin is said to be the inactive part (perhaps hypermethylated) and the only part of the chromatin, that can be stained. Euchromatin on the other side is the active part and can't be stained. The question is: What stain, what dye? Does this refer to hemalaun? Or to the dyes, that are used for bandering the chromosomes (Giemsa, ..)? Perhaps a silly question, but I hope, someone can help. Gudrun Lang From mtc205 <@t> Lehigh.EDU Tue Mar 17 16:06:19 2009 From: mtc205 <@t> Lehigh.EDU (Matthew T Close) Date: Tue Mar 17 16:06:24 2009 Subject: [Histonet] re:Hematoxylin Staining of Skin Message-ID: <4070641.1237323979958.JavaMail.mtc205@lehigh.edu> My first question is in what region of skin is the staining poor? The fibroblasts of the dermis should stain really well with H&E should the cells of the stratum germinativum. From there out nucle ar staining gets worse and worse. I attribute it to two things, and i problem: dividing and are g their way out to the stratum be sparse as a result of the process keratinized epithelia tend to be problematic for staining techniques where tissue has to be dehydrated a rehydrated. I have always had trouble sectioning highly keratinize skin because of improper infiltration. Second is the organization o f chromatin in epidermal cells in general, which is seemingly very differen germinativum often does s With all that being said, I hav problems associated with H&E staining Sometimes it might be as simple as adding acetic acid to your hematoxylin to lower the pH.&nbs doesn't work, I typically will use iron alum or ferric chlorid believe 2-4% iron alum has worked relatively well for me when used) as hematoxylin. because anything holding t hematoxylin and will need to be differentia counterstaining. I have also had mild success with iron ga elastin stain, but this is really more of a stain for elastin that ju st happens to stain nuclei blue-black and gives good differentiation. As I said behind this or maybe ev epidermal cells, the histo community -Matt From AnthonyH <@t> chw.edu.au Tue Mar 17 17:31:20 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 17 17:31:26 2009 Subject: [Histonet] Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues. In-Reply-To: <49BF6631020000B5000042F0@ekhgwia.ekht.nhs.uk> Message-ID: Sarah, Skin surface tends to dry out as well as the epithelial cells lose their nuclei as they migrate to the surface. The surface can also dry out after the application of antiseptics etc prior to biopsy. AND there is often the issue of the biopsy being allowed to dry prior to fixation. This will affect the haematoxylin staining. To improve the staining, try pretreating the dewaxed sections with 1% periodic acid for 10 minutes prior to H&E staining. This might work. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SARAH REEVES Sent: Tuesday, 17 March 2009 7:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues. Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues. ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Mar 17 17:39:05 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 17 17:39:10 2009 Subject: [Histonet] RE: DAB In-Reply-To: Message-ID: I have found the following method easy to use. The DAB Concentrate is aliquoted in small volumes and frozen. It is quite stable for at least a year. The following method is also used by our Haematology department for their myeloperoxidase staining: Peroxidase Peroxidase catalyses the transfer of hydrogen atoms from donor (hydrogen peroxide) to acceptor substances. One of the most common acceptor substances is 3,3 diaminobenzidine (DAB) which produces an insoluble, alcohol fast, brown precipitate at the site of the enzyme (Perkins, Henwood & Hewson 1985 ?Ultrastructural Myeloperoxidase Localisation: Applied to Routine Characterisation of Undifferentiated Haemopoletic Malignancies?. Aust. J. Med. Lab. Sc., 6(3):73-76.). The reaction can be made specific for eosinophils with the addition of sodium azide (Ahlstrom-Emanuelsson et al 2004 Eur Respir J 24:750-757). Fixation and Sectioning: Air dried unfixed 8?m cryostat sections Reagents 1. Phosphate Buffer: Potassium Dihydrogen Phosphate 2.622 g Sodium Carbonate 0.778 g Make up to 100ml. Final pH: 7.2 ? 0.2 at 25?C 2. DAB Stock Solution Caution Irritant 1. Weigh out 1g 3,3, Diaminobenzidine Tetrahydrochloride Grade II (Sigma D5637) in a fume hood 2. Dissolve in 25ml Distilled water 3. Label fifty 1ml reagent vials 4. Aliquot 0.5ml DAB solution in each tube 5. Freeze and Store at ?20oC 3. DAB Working Solution: 1. Defrost one vial of DAB Stock. 2. Add to 50ml Phosphate buffer in a coplin jar 3. Add 50?l Hydrogen Peroxide 4. Mix 5. Solution remains active for 2 hours Procedure 1. Air dry smears (maximum 1hr or alternatively store at -80oC until ready to stain) 2. Incubate slides for 5-7min until sections appear brown 3. Wash in water 2-3 minutes. 4. Counterstain with Haematoxylin 5. Dehydrate, clear and mount Results Peroxidase positive cells yellow-brown Nuclei Blue Controls ? Incubate sections in the Incubation medium omitting the hydrogen peroxide ? To specifically demonstrate eosinophil peroxidase add sodium azide to the incubation medium to a concentration of 0.005% Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Swain, Frances L Sent: Wednesday, 18 March 2009 2:43 AM To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: DAB There was a discussion about the Stable DAB on the histonet a few weeks ago. I would suggest you search the archieves of the histonet. The company that has this product was listed at that time. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, March 17, 2009 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB Greetings I recall that years ago Biomeda had a product called Stable DAB. It was a ready-to-use product that was stored in the freezer. Does anyone know if it is still being produced and whom I should contact? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tifei <@t> foxmail.com Tue Mar 17 21:20:43 2009 From: tifei <@t> foxmail.com (TF) Date: Tue Mar 17 21:21:39 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIEJyZFUgaW1tdW5vc3RhaW4gb24gcmF0IGJyYWluIHNlY3Rpb24=?= References: <712531.41983.qm@web38002.mail.mud.yahoo.com> Message-ID: <200903181020378307260@foxmail.com> Abcam monoclonal rat-anti BrdU http://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html Just compare to other Abcam anti-BrdU, it has most citations at least. 2009-03-18 TF ???? Amy Lee ????? 2009-03-18 07:14:56 ???? tifei ??? ??? Re: [Histonet] BrdU immunostain on rat brain section Hi, Could you please tell me your antibody catalog #? Thanks again, Amy --- On Mon, 3/16/09, TF wrote: From: TF Subject: Re: [Histonet] BrdU immunostain on rat brain section To: "amylee779" , "histonet" Date: Monday, March 16, 2009, 7:10 PM ? hi, we worked a lot on this. I prefer to use 40 um frozen sections. Abcam anti-BrdU (rat sourced) is the best ever I know. It does not have high background with rat IgG. Some other mouse-/sheep- sourced BrdU are available. 2009-03-17 TF ???? Amy Lee ????? 2009-03-17 05:02:28 ???? histonet ??? ??? [Histonet] BrdU immunostain on rat brain section Hello, I was asked to do BrdU immunostain on rat brain tissue. Does anyone have experience of it? 1.Do you prefer frozen or paraffin? 2.Which antibody you recommend? 3.?f?35um frozen is requied, what thickness you will use if you want go for paraffin? ? ? Thanks, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Mar 17 21:24:40 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Mar 17 21:24:43 2009 Subject: [Histonet] No Reply from Dako In-Reply-To: <90354A475B420441B2A0396E5008D4965E301B@copc-sbs.COPC.local> Message-ID: <814844.24380.qm@web31305.mail.mud.yahoo.com> Well Tom, I'm not sure what's up with Dako, but if I were them, I sure would try to give some explanations, and mend some fences. I haven't seen any explanations from ANYONE who is associated with Dako. I wonder if anyone from the head office is monitoring the Histonet negativity about their company? Do they care? It is going to cost them a ton of money! Regards, Akemi Allison-Tacha BS, HT (ASCP) HTL --- On Thu, 3/12/09, Thomas Jasper wrote: > From: Thomas Jasper > Subject: [Histonet] Dako > To: histonet@lists.utsouthwestern.edu > Date: Thursday, March 12, 2009, 2:42 PM > OK, > > There've been plenty of shots taken at Dako...and from > what I can tell > rightly so. I agree with the statements about the > "good old days" and > all, and I would still like to believe Dako is a decent > antibody > company. > > So here's my question - How 'bout it Dako? Are you > going to take all of > this lying down? Do you have any response(s) at all? You > (collectively > as a company) probably owe a lot of these folks some sort > of > explanation. I find it hard to believe that Dako has opted > to adopt a > business model, which (from all appearances) has taken a > path of > self-destruction. And I don't care who the parent > company is now > (Danaher?) these anecdotes exemplify irresponsible handling > of business. > It certainly isn't necessary to have attended Harvard > (insert your > favorite) business school to draw this conclusion. > > I also believe that competition is good for the > marketplace. By having > Dako, a one-time leader in the field, basically > "tank" isn't good for > anyone. I'd like to see something salvaged here, but > it's not up to me. > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology > Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Mar 18 00:01:36 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 18 00:01:53 2009 Subject: [Histonet] succinate dehydrogenase protocol In-Reply-To: <166529.77764.qm@web33108.mail.mud.yahoo.com> Message-ID: We stain for SDH in fresh frozen mouse skeletal muscle using a kit from Sigma. Galina Deyneko Sent by: histonet-bounces@lists.utsouthwestern.edu 03/17/2009 12:45 PM Please respond to galinadeyneko@yahoo.com To histonet@lists.utsouthwestern.edu cc Subject [Histonet] succinate dehydrogenase protocol Dear Colleagues I should detect /stain Succinate dehydrogenase in mouse muscle non-fixed frozen sections. Could someone please share detailed protocol and sources of the reagents. Does it exist any kit for detection? I performed some on-line search but did not find a sufficient information. Thank you in advance. Galina Deyneko Novartis, Cambridge, MA 617-871-7613. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adam.galle <@t> student.unsw.edu.au Wed Mar 18 00:04:33 2009 From: adam.galle <@t> student.unsw.edu.au (Adam Galle) Date: Wed Mar 18 00:04:50 2009 Subject: [Histonet] Cutting fresh-frozen brains from 1 week old rat pups In-Reply-To: <200812131356.mBDDuUCt029507@smtp-dist.unsw.edu.au> References: <200812131356.mBDDuUCt029507@smtp-dist.unsw.edu.au> Message-ID: <6563BEB2CA46401592FC9AC2CA2E2FE0@AdamGallePC> Hi all, Currently I am working with brains from 7 day old rat pups, that undergo an hypoxic-ischemic injury (Levine/Vannucci technique). These brains are unfixed, frozen in isopentane and cut at 20um on a croystat. These brains are not cutting very well compared to an adult brain (potenially due to unfinished myelination?), they are very 'crumbly' for want of a better term and always have cracks or generally poor preservation of morphology. I have tried all the standard tricks of different temperatures, section thickness and knife angle to no avail. I am going to perfuse fix my next cohort of animals with PFA and then a 30% sucrose step to see if that helps, but I was hoping that someone out there would have some tips on cutting these immature brains. Thanks, Adam. Adam Galle, Neuropharmacology and Brain Injury Lab Department of Pharmacology School of Medical Sciences UNSW Sydney From nefff <@t> staff.uni-marburg.de Wed Mar 18 03:47:46 2009 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Wed Mar 18 03:47:53 2009 Subject: [Histonet] cryoprotection possible? Message-ID: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the "test"-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universit?t Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From ooitinghuay <@t> yahoo.com Wed Mar 18 07:33:01 2009 From: ooitinghuay <@t> yahoo.com (Th Ooi) Date: Wed Mar 18 07:33:06 2009 Subject: [Histonet] (no subject) Message-ID: <970802.83849.qm@web54108.mail.re2.yahoo.com> Hi, I just began to work?with the methy methacrylate embedding plastic section. I am facing the difficulities to attach the sample on the glass slide. ? I have tried using Haupts adhesive coated slides and also the bond-rite slide to attach my 5micron sample.?I am doing normal hematoxylin and eosin staining. The section always detach from the glass slide towards the end of the staining. ? I need to use the concentrated Haupts adhesive coated slide to reduce?the chance for the?section?from lift up from the glass slide. However, I observed the high and dirty background after the staining. ? I noticed that some users are using Bond-rite slides to attach their samples. I tried this. However, the sections always lifted up towards the end of the staining. I am not too sure whether is my technique that giving me this kind of problem. I prewarmed the Bond-rite slides and put my sections in the 55C waterbath. Then I fished the section using the Bond-rite slide and lay it down on 55C heater. And finally in the oven incubator for more than 18 hours at 55C before staining... ? I would appreciate if?there is?any suggestion that can help to attach the section on the glass slide. ? Thank you very much! ? Yours sincerely, Ooi Singapore From SwainFrancesL <@t> uams.edu Wed Mar 18 08:12:45 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Wed Mar 18 08:18:11 2009 Subject: [Histonet] cryoprotection possible? In-Reply-To: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> References: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> Message-ID: Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [nefff@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the "test"-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universit?t Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From anh2006 <@t> med.cornell.edu Wed Mar 18 08:41:28 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Mar 18 08:44:11 2009 Subject: [Histonet] cryoprotection possible? In-Reply-To: References: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> Message-ID: <1435534215-1237383864-cardhu_decombobulator_blackberry.rim.net-1332485825-@bxe1028.bisx.prod.on.blackberry> Good advice indeed. However, I don't recommend you rinse in water after sucrose. Sort of defeats the purpose. Instead if you need to remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. Also, glutaraldehyde fixation usually renders tissue difficult to immunostain. You have to consider whether your fixation is appropriate for your antigen/antibody. -----Original Message----- From: "Swain, Frances L" Date: Wed, 18 Mar 2009 08:12:45 To: Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [nefff@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the "test"-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universit?t Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Wed Mar 18 08:49:38 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Wed Mar 18 08:50:16 2009 Subject: [Histonet] cryoprotection possible? In-Reply-To: <1435534215-1237383864-cardhu_decombobulator_blackberry.rim.net-1332485825-@bxe1028.bisx.prod.on.blackberry> References: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> <1435534215-1237383864-cardhu_decombobulator_blackberry.rim.net-1332485825-@bxe1028.bisx.prod.on.blackberry> Message-ID: You are so correct, I forgot you do not rinse the sucrose out you blot it and then freeze. Sorry about that. I do not cut frozen sections very often and I forget. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Wednesday, March 18, 2009 8:41 AM To: Swain, Frances L; Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryoprotection possible? Good advice indeed. However, I don't recommend you rinse in water after sucrose. Sort of defeats the purpose. Instead if you need to remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. Also, glutaraldehyde fixation usually renders tissue difficult to immunostain. You have to consider whether your fixation is appropriate for your antigen/antibody. -----Original Message----- From: "Swain, Frances L" Date: Wed, 18 Mar 2009 08:12:45 To: Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [nefff@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the "test"-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universit?t Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From leiker <@t> buffalo.edu Wed Mar 18 08:57:16 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 18 08:57:21 2009 Subject: [Histonet] Cutting fresh-frozen brains from 1 week old rat pups In-Reply-To: <6563BEB2CA46401592FC9AC2CA2E2FE0@AdamGallePC> References: <200812131356.mBDDuUCt029507@smtp-dist.unsw.edu.au> <6563BEB2CA46401592FC9AC2CA2E2FE0@AdamGallePC> Message-ID: Definitely do at least that one sucrose cryopreservation step you mentioned. Even step up to it in 10% and 20% sucrose. --On Wednesday, March 18, 2009 4:04 PM +1100 Adam Galle wrote: > Hi all, > Currently I am working with brains from 7 day old rat pups, that undergo > an hypoxic-ischemic injury (Levine/Vannucci technique). These brains are > unfixed, frozen in isopentane and cut at 20um on a croystat. These brains > are not cutting very well compared to an adult brain (potenially due to > unfinished myelination?), they are very 'crumbly' for want of a better > term and always have cracks or generally poor preservation of morphology. > I have tried all the standard tricks of different temperatures, section > thickness and knife angle to no avail. I am going to perfuse fix my next > cohort of animals with PFA and then a 30% sucrose step to see if that > helps, but I was hoping that someone out there would have some tips on > cutting these immature brains. > > Thanks, > Adam. > > > Adam Galle, > > Neuropharmacology and Brain Injury Lab > Department of Pharmacology > School of Medical Sciences > UNSW Sydney > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From ratliffjack <@t> hotmail.com Wed Mar 18 08:57:31 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Mar 18 08:57:35 2009 Subject: [Histonet] (no subject) In-Reply-To: <970802.83849.qm@web54108.mail.re2.yahoo.com> References: <970802.83849.qm@web54108.mail.re2.yahoo.com> Message-ID: Ooi, As you know, working with MMA is tricky enough to have to worry about losing sections during staining. Unfortunately, I cannot speak with experience in using Bond-rite slides, but maybe there is someone monitoring this message thread that can provide helpful information on that topic. However, I can speak to the experience of using Haupts coated slides. First, it is not uncommon to observe hematoxylin background staining from Haupts coated slides. Second, you are correct in that it takes just the right concentration of Haupts solution in order to retain section adherence to the slide. Given these two observations, allow me to provide a few suggestions for working with Haupts coated slides. Before I prepare my slides, it is necessary to prepare a working dilution of Haupts solution. Whether or not you use in house prepared or commercially prepared concentrate, you should typically look to a ratio of 1:1 with liquified concentrate to 50% EtOH. This is a pretty standard dilution that works for me. You definitely do not want to go higher than a 50% concentration of Haupts, but you could try tweaking it a little within the 40-50% range. I would suggest that maybe you do a short experiment using different dilutions of the Haupts concentrate so that you may zero in on that one dilution that will minimize background staining and optimize section adherence. You may not have perfection with the hematoxylin background or aniline blue in a trichrome stain (I usually substitue aniline blue with SF light green yellowish), but you may find something that you can live with for the H&E. Next, you might then try to work with the hematoxylin staining a bit. Specifically, maybe you can tweak the hematoxylin staining time by keeping it standard (I use hematoxylin 2 from Richard Allen and typically stain for 3 minutes) and/or increasing it a bit so that you can extend the acid clarifier step (I clarify or decolorize for 20 seconds) a little longer to help wash out or decolorize the background staining. Obviously you do not want to compromise your nuclear detail, but this should help a bit. Please feel free to ask any additional questions as needed. Jack Ratliff > Date: Wed, 18 Mar 2009 05:33:01 -0700 > From: ooitinghuay@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi, I just began to work with the methy methacrylate embedding plastic section. I am facing the difficulities to attach the sample on the glass slide. > > I have tried using Haupts adhesive coated slides and also the bond-rite slide to attach my 5micron sample. I am doing normal hematoxylin and eosin staining. The section always detach from the glass slide towards the end of the staining. > > I need to use the concentrated Haupts adhesive coated slide to reduce the chance for the section from lift up from the glass slide. However, I observed the high and dirty background after the staining. > > I noticed that some users are using Bond-rite slides to attach their samples. I tried this. However, the sections always lifted up towards the end of the staining. I am not too sure whether is my technique that giving me this kind of problem. I prewarmed the Bond-rite slides and put my sections in the 55C waterbath. Then I fished the section using the Bond-rite slide and lay it down on 55C heater. And finally in the oven incubator for more than 18 hours at 55C before staining... > > I would appreciate if there is any suggestion that can help to attach the section on the glass slide. > > Thank you very much! > > Yours sincerely, > Ooi > Singapore > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SLB <@t> stowers.org Wed Mar 18 08:57:18 2009 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Wed Mar 18 08:58:00 2009 Subject: [Histonet] cryoprotection possible? In-Reply-To: Message-ID: What I do is take the specimen out of 30% sucrose, blot and place in OCT for maybe 30-60 minutes, then place in fresh OCT and freeze. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Swain, Frances L Sent: Wednesday, March 18, 2009 8:50 AM To: anh2006@med.cornell.edu; Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? You are so correct, I forgot you do not rinse the sucrose out you blot it and then freeze. Sorry about that. I do not cut frozen sections very often and I forget. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Wednesday, March 18, 2009 8:41 AM To: Swain, Frances L; Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryoprotection possible? Good advice indeed. However, I don't recommend you rinse in water after sucrose. Sort of defeats the purpose. Instead if you need to remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. Also, glutaraldehyde fixation usually renders tissue difficult to immunostain. You have to consider whether your fixation is appropriate for your antigen/antibody. -----Original Message----- From: "Swain, Frances L" Date: Wed, 18 Mar 2009 08:12:45 To: Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [nefff@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the "test"-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universit?t Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Mar 18 09:03:02 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Mar 18 09:03:09 2009 Subject: [Histonet] cryoprotection possible? In-Reply-To: References: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> <1435534215-1237383864-cardhu_decombobulator_blackberry.rim.net-1332485825-@ bxe1028.bisx.prod.on.blackberry> Message-ID: I agree with Frances and anh2006. Cryospreservation is important for brains especially and must be done after fixation, before freezing, and the glutaraldeyde can irreversibly mask antigens. Merced --On Wednesday, March 18, 2009 8:49 AM -0500 "Swain, Frances L" wrote: > You are so correct, I forgot you do not rinse the sucrose out you blot it > and then freeze. Sorry about that. I do not cut frozen sections very > often and I forget. > > Frances L. Swain HT(ASCP) A. A. S. > Special Procedures Technician > Department of Orthopaedic Surgery > Center for Orthopaedic Research > Barton Research Building 2R28 > 4301 West Markham Street > Little Rock AR 72205 > (501) 686-8739 PHONE > (501) 686-8987 FAX > swainfrancesl@uams.edu email > -----Original Message----- > From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] > Sent: Wednesday, March 18, 2009 8:41 AM > To: Swain, Frances L; Dr. med. Frauke Neff; > histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryoprotection > possible? > > Good advice indeed. However, I don't recommend you rinse in water after > sucrose. Sort of defeats the purpose. Instead if you need to remove > excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. > > Also, glutaraldehyde fixation usually renders tissue difficult to > immunostain. You have to consider whether your fixation is appropriate > for your antigen/antibody. > > > -----Original Message----- > From: "Swain, Frances L" > > Date: Wed, 18 Mar 2009 08:12:45 > To: Dr. med. Frauke Neff; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] cryoprotection possible? > > > Usually the cryoprotection is carried out after the specimens are fixed > and before they are frozen. If you have a sample you can spare you might > try making up some 20% Sucrose placing the frozen sample in it. putting > it in the refrigerator and letting it stay in the 20% sucrose until it > drops to the bottom of the container. You might have to change the 20% > Sucrose a couple of times as you know sucrose can grow bacteria easily. > When the specimen has dropped to the bottom of the container, remove it, > rinse in a couple of changes of distilled water and quick freeze the > sample. Try cutting and staining the sample and see if the morphology is > good if it is then you can do all of your samples to correct the problem. > There should be no thawing between removal of the sample from the storage > to the cryostat. Hope this helps > Frances Swain > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke > Neff [nefff@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryoprotection possible? > > Dear Histonetters, > I'm supposed to do a noradrenaline ihc on rat brains that have been snap > frozen and afterwards glutaraldehyd fixed. While the morphology is okay > in the "test"-tissue we used to establish the protocol, it was very poor > in the tissue of the trial animals. > It appeared mooth eaten and disrupted. > > I assume the brains were thawed and refrosted due to moving the samples > between several cities. Does anyone know a method /a tip how I can save > some proper morphology? > > I checked the archive and found sucrose as cryoprotectant, but this is > supposed to do it while the tissue is frozen or is it possible to use it > while the tissue thaws? > > Thank you all in advance for your patience and help, > > > Frauke > > > -- > Dr. med. Frauke Neff > AG Neurologische Therapieforschung > Neurologie > Philipps-Universit?t Marburg > Rudolph-Bultmann Str. 8 > 35039 Marburg > 06421/5866304 > > > > > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From nefff <@t> staff.uni-marburg.de Wed Mar 18 09:07:32 2009 From: nefff <@t> staff.uni-marburg.de (nefff@staff.uni-marburg.de) Date: Wed Mar 18 09:07:59 2009 Subject: [Histonet] cryoprotection possible? In-Reply-To: <1435534215-1237383864-cardhu_decombobulator_blackberry.rim.net-1332485825-@bxe1028.bisx.prod.on.blackberry> References: <1237366066.49c0b53228f92@webmail.med.uni-marburg.de> <1435534215-1237383864-cardhu_decombobulator_blackberry.rim.net-1332485825-@bxe1028.bisx.prod.on.blackberry> Message-ID: <20090318150732.vsxsh78w8404s8c8@home.staff.uni-marburg.de> We tried nearly everything for the noradreanline IHC and did't get any staining. After several calls with the techs of the company, they told me I've to do a glutaraldehyd fixation, every other fixation will wash the noradrenaline out of the tissue (even the cryosection did't give a stain). Using adrenal glands as positive control, I got a weak positive stain using the GA-Fixation. But I'm not happy with this, because the samples I got are FFPE or snap frozen. So what to do to proceed? Because I don't know any way back through the formalin fixation, I decided to go on with the snap frozen tissue. I would be very happy, if someone would tell me: "Hey, thats all bullshit, you just need the antibody XXX of the company YYY and verything will work fine with this noradrenaline IHC" Until this happens, I try with the snap-frozen material, keep my fingers cross, throw pennies in every fontaine, rub oil-lamps and lion noses.... You see, I'm desperately needing help, Frauke Zitat von anh2006@med.cornell.edu: > Good advice indeed. However, I don't recommend you rinse in water > after sucrose. Sort of defeats the purpose. Instead if you need to > remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. > > Also, glutaraldehyde fixation usually renders tissue difficult to > immunostain. You have to consider whether your fixation is > appropriate for your antigen/antibody. > > > -----Original Message----- > From: "Swain, Frances L" > > Date: Wed, 18 Mar 2009 08:12:45 > To: Dr. med. Frauke Neff; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] cryoprotection possible? > > > Usually the cryoprotection is carried out after the specimens are > fixed and before they are frozen. If you have a sample you can > spare you might try making up some 20% Sucrose placing the frozen > sample in it. putting it in the refrigerator and letting it stay in > the 20% sucrose until it drops to the bottom of the container. You > might have to change the 20% Sucrose a couple of times as you know > sucrose can grow bacteria easily. When the specimen has dropped to > the bottom of the container, remove it, rinse in a couple of changes > of distilled water and quick freeze the sample. Try cutting and > staining the sample and see if the morphology is good if it is then > you can do all of your samples to correct the problem. There should > be no thawing between removal of the sample from the storage to the > cryostat. > Hope this helps > Frances Swain > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. > Frauke Neff [nefff@staff.uni-marburg.de] > Sent: Wednesday, March 18, 2009 3:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryoprotection possible? > > Dear Histonetters, > I'm supposed to do a noradrenaline ihc on rat brains that have been > snap frozen > and afterwards glutaraldehyd fixed. While the morphology is okay in the > "test"-tissue we used to establish the protocol, it was very poor in > the tissue > of the trial animals. > It appeared mooth eaten and disrupted. > > I assume the brains were thawed and refrosted due to moving the > samples between > several cities. Does anyone know a method /a tip how I can save some proper > morphology? > > I checked the archive and found sucrose as cryoprotectant, but this > is supposed > to do it while the tissue is frozen or is it possible to use it while the > tissue thaws? > > Thank you all in advance for your patience and help, > > > Frauke > > > -- > Dr. med. Frauke Neff > AG Neurologische Therapieforschung > Neurologie > Philipps-Universit?t Marburg > Rudolph-Bultmann Str. 8 > 35039 Marburg > 06421/5866304 > > > > > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and > may contain confidential and privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From joost.bruijntjes <@t> tno.nl Wed Mar 18 09:48:00 2009 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Wed Mar 18 09:48:07 2009 Subject: [Histonet] FW: CD86 - mouse Message-ID: <8865601DD17A554CB489C17FFD8A51B20230792E@MAIL04.tsn.tno.nl> Hi histonetters Is really nobody working with cd86 on mouse tissues? Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From tbraud <@t> holyredeemer.com Wed Mar 18 09:54:31 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Mar 18 09:54:36 2009 Subject: [Histonet] RE: CLIA vs CAP grossing In-Reply-To: <8c1f15600001e1a9@HolyRedeemer.com> Message-ID: CAP has deemed status from CLIA, so the CAP definition should be accepted. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax Message: 3 Date: Tue, 17 Mar 2009 11:16:22 -0600 From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Grossing CAP is OK with their guidelines ( BUTTTTTTTTT is CLIA on line with the Processing (Low Complexity?) vs Grossing (High Complexity?). If CLIA is not on board with processing (dumb naming) being low complexity and grossing being higher complexity your lab may be in violation of CLIA Guidelines. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com **************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From cmiller <@t> physlab.com Wed Mar 18 11:50:16 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Mar 18 11:50:21 2009 Subject: [Histonet] Mesothelioma control tissue Message-ID: <001701c9a7e9$9d9d2570$4402a8c0@plab.local> Hi Histonetter's, I am in need of a mesothelioma control block for my IHC. Is there anyone that would be willing to share? I might have tissue that you may need as well. Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From AFoshey <@t> chw.org Wed Mar 18 11:51:01 2009 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Wed Mar 18 11:51:05 2009 Subject: [Histonet] Snap freezing muscle tissue for metabolic testing Message-ID: Fellow histonetters: The ordering physicians/neurologists are saying that the metabolic testing is not accurate because of the way the muscle tissue is being handled currently. Does anyone have specific references that would be helpful in the argument for implementing snap freezing muscle tissue in the OR verses the reference lab when the reference lab is less than 30 minutes from the OR? Thanks in advance, Annette Foshey, HT (ASCP) Charge person in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org From pdunlop720 <@t> gmail.com Wed Mar 18 12:09:40 2009 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Wed Mar 18 12:09:49 2009 Subject: [Histonet] equipment purchasing Message-ID: <80ab7bc60903181009o6e178432m42613f7e5cc0eb18@mail.gmail.com> Hello, Our histo lab is currently looking into buying one or all of the following: cassette labeler, automated stainer (that can also accommodate a few special stains), and automated coverslipper (that uses minimal xylene or no xylene). Can anyone recommend good brands and models that they like as well as what vendors might have good prices? We are also looking into getting the stainer and coverslipper either used or refurbished to cut down on costs. Our lab is small and we do only about 40-50 slides per day on average. We would prefer to have the equipment to be as small as possible. Any suggestions are welcome! Thanks, Patty From nfournier <@t> sasktel.net Wed Mar 18 14:18:23 2009 From: nfournier <@t> sasktel.net (Neil M. Fournier) Date: Wed Mar 18 14:18:29 2009 Subject: [Histonet] glycerol buffered antifade media Message-ID: Hi Everyone, I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol (pH to ~ 10.0 with NaOH) However, I notice that solution became quite a dark yellow when dissolving. Is this normal? It seems to occur every time I try to make it up. Thanks in advance Neil E-mail message checked by Spyware Doctor (6.0.0.386) Database version: 5.11990 http://www.pctools.com/en/spyware-doctor-antivirus/ From NMargaryan <@t> childrensmemorial.org Wed Mar 18 14:20:34 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Mar 18 14:20:50 2009 Subject: [Histonet] Double-stain for immune cell markers Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601E6CC03@CMHEXC01EVS.childrensmemorial.org> Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira From lscott <@t> sfcn.org Wed Mar 18 23:19:45 2009 From: lscott <@t> sfcn.org (Scott) Date: Wed Mar 18 23:20:09 2009 Subject: [Histonet] Paraffin Block triming Message-ID: <550661ED746D44B48C04DB86EB971C75@LesliePC> Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) From JMacDonald <@t> mtsac.edu Wed Mar 18 23:26:37 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 18 23:26:54 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <550661ED746D44B48C04DB86EB971C75@LesliePC> Message-ID: We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Mar 19 05:34:38 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 19 05:34:47 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <550661ED746D44B48C04DB86EB971C75@LesliePC> Message-ID: <1128894179.63154441237458878301.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum ----- Original Message ----- From: "Scott" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbpiontek <@t> hotmail.com Thu Mar 19 07:28:44 2009 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Thu Mar 19 07:28:48 2009 Subject: [Histonet] RE: Automating muscle and neuro stains In-Reply-To: References: Message-ID: I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail?. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_WL_HM_express_032009#colortheme From akbitting <@t> geisinger.edu Thu Mar 19 08:13:46 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Mar 19 08:14:02 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: References: <550661ED746D44B48C04DB86EB971C75@LesliePC> Message-ID: <49C20CCA.2B7F.00C9.0@geisinger.edu> I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From rjbuesa <@t> yahoo.com Thu Mar 19 08:25:19 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 19 08:25:24 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <49C20CCA.2B7F.00C9.0@geisinger.edu> Message-ID: <767749.47598.qm@web65708.mail.ac4.yahoo.com> You will have to time worself as I did to find out that waiting fo?the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs.?80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Mar 19 08:31:21 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 19 08:31:26 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <49C20CCA.2B7F.00C9.0@geisinger.edu> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C20CCA.2B7F.00C9.0@geisinger.edu> Message-ID: <000c01c9a896$fe832c60$095a5b82@vet.upenn.edu> Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From carrolpb <@t> umdnj.edu Thu Mar 19 08:30:34 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Mar 19 08:31:35 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <550661ED746D44B48C04DB86EB971C75@LesliePC> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> Message-ID: <49C248FA.5060906@umdnj.edu> > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From KGroeger <@t> USLABS.net Thu Mar 19 08:37:46 2009 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Thu Mar 19 08:37:53 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <000c01c9a896$fe832c60$095a5b82@vet.upenn.edu> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C20CCA.2B7F.00C9.0@geisinger.edu> <000c01c9a896$fe832c60$095a5b82@vet.upenn.edu> Message-ID: They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-bounces@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Mar 19 08:45:46 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Mar 19 08:46:35 2009 Subject: [Histonet] Paraffin Block trimming References: <767749.47598.qm@web65708.mail.ac4.yahoo.com> Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C946D9@e2k3ms1.urmc-sh.rochester.edu> In my experience the paraffin block trimmer is quicker and easier on your hands than the old manual scraping method. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 3/19/2009 9:25 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Thu Mar 19 08:56:46 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Mar 19 08:56:50 2009 Subject: [Histonet] Paraffin Block triming References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu> Message-ID: <769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Mar 19 08:58:25 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 19 08:58:58 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu> <769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Thu Mar 19 09:03:08 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Mar 19 09:03:15 2009 Subject: [Histonet] Paraffin Block trimming Message-ID: <32862.36509.qm@web58608.mail.re3.yahoo.com> My techs use our Para Trimmer from Thermo Scientific Shandon.?It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least?twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 3/19/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo?the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs.?80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab.? It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? Thanks, Scott Hendricksen? HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu Mar 19 09:08:40 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Mar 19 09:09:11 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C20CCA.2B7F.00C9.0@geisinger.edu><000c01c9a896$fe832c60$095a5b82@vet.upenn.edu> Message-ID: <8CB76B3572A802B-1288-30F1@webmail-md03.sysops.aol.com> I agree that this is quicker than manually---you can do 4?or 5 blocks at a time. -----Original Message----- From: Karin Groeger To: Pamela Marcum ; Angela Bitting ; histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald ; Scott Cc: Histonet@lists.utsouthwestern.edu Sent: Thu, 19 Mar 2009 9:37 am Subject: RE: [Histonet] Paraffin Block trimming They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-bounces@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anyb ody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this me ssage including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Mar 19 09:24:00 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Mar 19 09:26:24 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <32862.36509.qm@web58608.mail.re3.yahoo.com> References: <32862.36509.qm@web58608.mail.re3.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BD20@LRGHEXVS1.practice.lrgh.org> We have one timmer. I don't use it, I hand trim simply because I am quicker with it. The other techs use the timmer to save their hands. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhisto@yahoo.com] Sent: Thursday, March 19, 2009 10:03 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting; rjbuesa@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From gmartin <@t> marshallmedical.org Thu Mar 19 09:37:13 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Mar 19 09:38:18 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> Message-ID: <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Mar 19 09:38:58 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Mar 19 09:52:26 2009 Subject: FW: [Histonet] RE: Automating muscle and neuro stains Message-ID: <57D70B824966452FA0D788FD34277E38@IBLS.GLA.AC.UK> Have I died and gone to histologists heaven? Automate an ATPase, now there would be something special. No more crying and wailing over failed or partially successful acid reversal. I'll have to retreat to a darkened room, I'm traumatised. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. Subject: RE: [Histonet] RE: Automating muscle and neuro stains I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for HotmailR. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX _WL_HM_express_032009#colortheme____________________________________________ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbruce <@t> vetpathservicesinc.com Thu Mar 19 09:55:20 2009 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Thu Mar 19 09:55:31 2009 Subject: [Histonet] Sanderson's RBS Image Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2855191@vpss1.VetPathServicesInc.local> Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? Thanks in advance, Suzanne _______________________________ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator From leiker <@t> buffalo.edu Thu Mar 19 09:55:36 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Mar 19 09:55:44 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> Message-ID: <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" wrote: > No you're not the only one ... I was wondering the same thing ... why all > the scraping. It seems to me that clean embedding does the trick with a > few exceptions. G > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to scrape > a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape from > the blocks. ;) > > But, I?do scrape using the handle end of?the same forceps I use to pick > up the ribbon and tease the sections. No sharp edge. No electricity. > > PKP > > > ? > > > > ________________________________ > From: Peter Carroll > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I find > that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >> time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen? HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From jqb7 <@t> cdc.gov Thu Mar 19 09:57:12 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 19 09:58:21 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E3@LTA3VS011.ees.hhs.gov> Don't know what to say.........I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Merced Leiker [mailto:leiker@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" wrote: > No you're not the only one ... I was wondering the same thing ... why all > the scraping. It seems to me that clean embedding does the trick with a > few exceptions. G > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to scrape > a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape from > the blocks. ;) > > But, I?do scrape using the handle end of?the same forceps I use to pick > up the ribbon and tease the sections. No sharp edge. No electricity. > > PKP > > > ? > > > > ________________________________ > From: Peter Carroll > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I find > that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >> time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen? HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From carrolpb <@t> umdnj.edu Thu Mar 19 10:05:12 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Mar 19 10:07:02 2009 Subject: [Histonet] Sanderson's RBS Image In-Reply-To: <26DB1FDFBF9EE14AB3AACC873519A4A2855191@vpss1.VetPathServicesInc.local> References: <26DB1FDFBF9EE14AB3AACC873519A4A2855191@vpss1.VetPathServicesInc.local> Message-ID: <49C25F28.7020701@umdnj.edu> > Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? No, but Google does. About a half a second of searching found these... http://www.surgipath.com/products/itm_photos/p111d26-large.jpg http://archotol.ama-assn.org/cgi/reprint/129/10/1125.pdf Suzanne Bruce wrote: > Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? > > Thanks in advance, > Suzanne > > _______________________________ > Suzanne Bruce, R.V.T. > Histologist & Necropsy Coordinator > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Shakun.Aswani <@t> acologix.com Thu Mar 19 10:08:35 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Thu Mar 19 10:08:44 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com> I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khicks71 <@t> comcast.net Thu Mar 19 10:10:49 2009 From: khicks71 <@t> comcast.net (khicks71@comcast.net) Date: Thu Mar 19 10:10:52 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> Message-ID: <1201255339.6191081237475449211.JavaMail.root@sz0061a.emeryville.ca.mail.comcast.net> The lab I work at part time uses the new paraffin trimmer by Surgipath, it is alot bigger than the one I have seen before in other labs.?You can do approx. 8 blocks at a time.?But I also agree, if you "clean" embedd, you do not have all that extra paraffin to clean up. Kathy Hicks H.T. (ASCP) DPNS Surgical Center 400 SKokie Blvd. Suite 450 Northbrook, Illinois? 60062 From KKay <@t> chr.ab.ca Thu Mar 19 10:10:41 2009 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Thu Mar 19 10:10:57 2009 Subject: [Histonet] Paraffin block - cassette cleaner In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494F33@exbe.chr.ab.ca> Hello, We have the Thermo Fisher model as well and love it. It does an excellent job on the blocks.in much less time than traditional hand scraping. Karen J Kay, MLT Pathology Supervisor,Chinook Health Laboratory Chinook Regional Hospital,Lethbridge, Alberta, CANADA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: March 19, 2009 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. equipment purchasing (Patty Dunlop) 2. glycerol buffered antifade media (Neil M. Fournier) 3. Double-stain for immune cell markers (Margaryan, Naira) 4. Paraffin Block triming (Scott) 5. Re: Paraffin Block trimming (Jennifer MacDonald) 6. Re: Paraffin Block triming (Pamela Marcum) 7. RE: RE: Automating muscle and neuro stains (Denise Piontek) 8. Re: Paraffin Block trimming (Angela Bitting) 9. Re: Paraffin Block trimming (Rene J Buesa) 10. RE: Paraffin Block trimming (Pamela Marcum) 11. Re: Paraffin Block triming (Peter Carroll) 12. RE: Paraffin Block trimming (Karin Groeger) 13. RE: Paraffin Block trimming (McMahon, Loralee A) 14. Paraffin Block triming (Paula Pierce) 15. RE: Paraffin Block triming (Bartlett, Jeanine (CDC/CCID/NCZVED)) 16. Re: Paraffin Block trimming (Kelly Boyd) 17. Re: Paraffin Block trimming (godsgalnow@aol.com) 18. RE: Paraffin Block trimming (Podawiltz, Thomas) 19. RE: Paraffin Block trimming (Martin, Gary) 20. FW: [Histonet] RE: Automating muscle and neuro stains (Ian Montgomery) 21. Sanderson's RBS Image (Suzanne Bruce) 22. RE: Paraffin Block trimming (Merced Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Mar 2009 10:09:40 -0700 From: Patty Dunlop Subject: [Histonet] equipment purchasing To: histonet@lists.utsouthwestern.edu Message-ID: <80ab7bc60903181009o6e178432m42613f7e5cc0eb18@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello, Our histo lab is currently looking into buying one or all of the following: cassette labeler, automated stainer (that can also accommodate a few special stains), and automated coverslipper (that uses minimal xylene or no xylene). Can anyone recommend good brands and models that they like as well as what vendors might have good prices? We are also looking into getting the stainer and coverslipper either used or refurbished to cut down on costs. Our lab is small and we do only about 40-50 slides per day on average. We would prefer to have the equipment to be as small as possible. Any suggestions are welcome! Thanks, Patty ------------------------------ Message: 2 Date: Wed, 18 Mar 2009 13:18:23 -0600 From: "Neil M. Fournier" Subject: [Histonet] glycerol buffered antifade media To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Everyone, I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol (pH to ~ 10.0 with NaOH) However, I notice that solution became quite a dark yellow when dissolving. Is this normal? It seems to occur every time I try to make it up. Thanks in advance Neil E-mail message checked by Spyware Doctor (6.0.0.386) Database version: 5.11990 http://www.pctools.com/en/spyware-doctor-antivirus/ ------------------------------ Message: 3 Date: Wed, 18 Mar 2009 14:20:34 -0500 From: "Margaryan, Naira" Subject: [Histonet] Double-stain for immune cell markers To: Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601E6CC03@CMHEXC01EVS.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira ------------------------------ Message: 4 Date: Wed, 18 Mar 2009 22:19:45 -0600 From: "Scott" Subject: [Histonet] Paraffin Block triming To: Message-ID: <550661ED746D44B48C04DB86EB971C75@LesliePC> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ------------------------------ Message: 5 Date: Wed, 18 Mar 2009 21:26:37 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Paraffin Block trimming To: "Scott" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 19 Mar 2009 06:34:38 -0400 (EDT) From: Pamela Marcum Subject: Re: [Histonet] Paraffin Block triming To: Scott Cc: Histonet@lists.utsouthwestern.edu Message-ID: <1128894179.63154441237458878301.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Content-Type: text/plain; charset=utf-8 Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum ----- Original Message ----- From: "Scott" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 19 Mar 2009 08:28:44 -0400 From: Denise Piontek Subject: RE: [Histonet] RE: Automating muscle and neuro stains To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail?. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_WL_HM_express_032009#colortheme ------------------------------ Message: 8 Date: Thu, 19 Mar 2009 09:13:46 -0400 From: "Angela Bitting" Subject: Re: [Histonet] Paraffin Block trimming To: , "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <49C20CCA.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 9 Date: Thu, 19 Mar 2009 06:25:19 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, Jennifer MacDonald , Scott , Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Message-ID: <767749.47598.qm@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You will have to time worself as I did to find out that waiting fo?the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs.?80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 19 Mar 2009 09:31:21 -0400 From: "Pamela Marcum" Subject: RE: [Histonet] Paraffin Block trimming To: "'Angela Bitting'" , , "'Jennifer MacDonald'" , "'Scott'" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <000c01c9a896$fe832c60$095a5b82@vet.upenn.edu> Content-Type: text/plain; charset="US-ASCII" Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ------------------------------ Message: 11 Date: Thu, 19 Mar 2009 09:30:34 -0400 From: Peter Carroll Subject: Re: [Histonet] Paraffin Block triming To: histonet@lists.utsouthwestern.edu Message-ID: <49C248FA.5060906@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save > any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 12 Date: Thu, 19 Mar 2009 06:37:46 -0700 From: "Karin Groeger" Subject: RE: [Histonet] Paraffin Block trimming To: "Pamela Marcum" , "Angela Bitting" , , "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-bounces@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ------------------------------ Message: 13 Date: Thu, 19 Mar 2009 09:45:46 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] Paraffin Block trimming To: , , "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C946D9@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" In my experience the paraffin block trimmer is quicker and easier on your hands than the old manual scraping method. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 3/19/2009 9:25 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 19 Mar 2009 06:56:46 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Paraffin Block triming To: Histonet Message-ID: <769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 19 Mar 2009 09:58:25 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Paraffin Block triming To: "Paula Pierce" , Histonet Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=iso-8859-1 I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 19 Mar 2009 07:03:08 -0700 (PDT) From: Kelly Boyd Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, Jennifer MacDonald , Scott , Angela Bitting , rjbuesa@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Message-ID: <32862.36509.qm@web58608.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My techs use our Para Trimmer from Thermo Scientific Shandon.?It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least?twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 3/19/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo?the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs.?80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab.? It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? Thanks, Scott Hendricksen? HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 19 Mar 2009 10:08:40 -0400 From: godsgalnow@aol.com Subject: Re: [Histonet] Paraffin Block trimming To: KGroeger@USLABS.net, pmarcum@vet.upenn.edu, akbitting@geisinger.edu, histonet-bounces@lists.utsouthwestern.edu, JMacDonald@mtsac.edu, lscott@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Message-ID: <8CB76B3572A802B-1288-30F1@webmail-md03.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I agree that this is quicker than manually---you can do 4?or 5 blocks at a time. -----Original Message----- From: Karin Groeger To: Pamela Marcum ; Angela Bitting ; histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald ; Scott Cc: Histonet@lists.utsouthwestern.edu Sent: Thu, 19 Mar 2009 9:37 am Subject: RE: [Histonet] Paraffin Block trimming They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-bounces@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anyb ody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this me ssage including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 19 Mar 2009 10:24:00 -0400 From: "Podawiltz, Thomas" Subject: RE: [Histonet] Paraffin Block trimming To: Kelly Boyd , "histonet-bounces@lists.utsouthwestern.edu" , Jennifer MacDonald , Scott , Angela Bitting , "rjbuesa@yahoo.com" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BD20@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="iso-8859-1" We have one timmer. I don't use it, I hand trim simply because I am quicker with it. The other techs use the timmer to save their hands. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhisto@yahoo.com] Sent: Thursday, March 19, 2009 10:03 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting; rjbuesa@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 19 Date: Thu, 19 Mar 2009 07:37:13 -0700 From: "Martin, Gary" Subject: RE: [Histonet] Paraffin Block trimming To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , "Paula Pierce" , "Histonet" Message-ID: <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="iso-8859-1" No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 19 Mar 2009 14:38:58 -0000 From: "Ian Montgomery" Subject: FW: [Histonet] RE: Automating muscle and neuro stains To: Message-ID: <57D70B824966452FA0D788FD34277E38@IBLS.GLA.AC.UK> Content-Type: text/plain; charset="us-ascii" Have I died and gone to histologists heaven? Automate an ATPase, now there would be something special. No more crying and wailing over failed or partially successful acid reversal. I'll have to retreat to a darkened room, I'm traumatised. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. Subject: RE: [Histonet] RE: Automating muscle and neuro stains I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for HotmailR. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX _WL_HM_express_032009#colortheme____________________________________________ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 19 Mar 2009 10:55:20 -0400 From: "Suzanne Bruce" Subject: [Histonet] Sanderson's RBS Image To: "histonet" Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2855191@vpss1.VetPathServicesInc.local> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? Thanks in advance, Suzanne _______________________________ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator ------------------------------ Message: 22 Date: Thu, 19 Mar 2009 10:55:36 -0400 From: Merced Leiker Subject: RE: [Histonet] Paraffin Block trimming To: "Martin, Gary" , "Bartlett, Jeanine (CDC/CCID/NCZVED)" , Paula Pierce , Histonet Message-ID: <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" wrote: > No you're not the only one ... I was wondering the same thing ... why > all the scraping. It seems to me that clean embedding does the trick > with a few exceptions. G > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 > 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to > scrape a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape > from the blocks. ;) > > But, I?do scrape using the handle end of?the same forceps I use to > pick up the ribbon and tease the sections. No sharp edge. No > electricity. > > PKP > > > ? > > > > ________________________________ > From: Peter Carroll > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I > find that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save >> any time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen? HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 64, Issue 33 **************************************** This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From leiker <@t> buffalo.edu Thu Mar 19 10:15:46 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Mar 19 10:15:52 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E3@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E3@LTA3VS011.ees.hhs.gov> Message-ID: <96B125D72FDDF2F702DA3E43@bchwxp2702.ad.med.buffalo.edu> hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: > Don't know what to say.........I fill mine up and as long as I don't get > to rough with it (bump it or something where it sloshes) I don't have any > excess paraffin around the edges. I use metal base molds. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Merced Leiker [mailto:leiker@buffalo.edu] > Sent: Thursday, March 19, 2009 10:56 AM > To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; > Histonet Subject: RE: [Histonet] Paraffin Block trimming > > Now I'm interested in how you embed so as to not have to scrape...if i > don't add enough wax (enough to end up rising around the edges of the > cassette, hence the scraping later), i don't get a secure hold of the > block to the cassette... > > ML > > --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" > wrote: > >> No you're not the only one ... I was wondering the same thing ... why all >> the scraping. It seems to me that clean embedding does the trick with a >> few exceptions. G >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM >> To: Paula Pierce; Histonet >> Subject: RE: [Histonet] Paraffin Block triming >> >> I was wondering if I was the only one out there that rarely has to scrape >> a block. >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >> Pierce Sent: Thursday, March 19, 2009 9:57 AM >> To: Histonet >> Subject: [Histonet] Paraffin Block triming >> >> I try to embed so as to have a minimal amount of paraffin to scrape from >> the blocks. ;) >> >> But, I?do scrape using the handle end of?the same forceps I use to pick >> up the ribbon and tease the sections. No sharp edge. No electricity. >> >> PKP >> >> >> ? >> >> >> >> ________________________________ >> From: Peter Carroll >> To: histonet@lists.utsouthwestern.edu >> Sent: Thursday, March 19, 2009 8:30:34 AM >> Subject: Re: [Histonet] Paraffin Block triming >> >>> Does anybody use a paraffin block dewaxer ? >> >> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find >> that it's not only very quick, but quite accurate... >> >> >> >> Scott wrote: >>> Hi, >>> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >>> time, how well does it work? >>> >>> Thanks, >>> >>> Scott Hendricksen? HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> ? >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Doug.Showers <@t> propath.com Thu Mar 19 10:16:20 2009 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Thu Mar 19 10:17:54 2009 Subject: [Histonet] Paraffin block - cassette cleaner In-Reply-To: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494F33@exbe.chr.ab.ca> References: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494F33@exbe.chr.ab.ca> Message-ID: <82C7248978CB50469FD6BA68EBBEFE67EB477B@exchange.propathlab.com> We use the ThermoFisher model also. It keeps the floor much cleaner, too, since the paraffin is melted into a small cup rather than flying around the counter and floor. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kay, Karen Sent: Thursday, March 19, 2009 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin block - cassette cleaner Hello, We have the Thermo Fisher model as well and love it. It does an excellent job on the blocks.in much less time than traditional hand scraping. Karen J Kay, MLT Pathology Supervisor,Chinook Health Laboratory Chinook Regional Hospital,Lethbridge, Alberta, CANADA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: March 19, 2009 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. equipment purchasing (Patty Dunlop) 2. glycerol buffered antifade media (Neil M. Fournier) 3. Double-stain for immune cell markers (Margaryan, Naira) 4. Paraffin Block triming (Scott) 5. Re: Paraffin Block trimming (Jennifer MacDonald) 6. Re: Paraffin Block triming (Pamela Marcum) 7. RE: RE: Automating muscle and neuro stains (Denise Piontek) 8. Re: Paraffin Block trimming (Angela Bitting) 9. Re: Paraffin Block trimming (Rene J Buesa) 10. RE: Paraffin Block trimming (Pamela Marcum) 11. Re: Paraffin Block triming (Peter Carroll) 12. RE: Paraffin Block trimming (Karin Groeger) 13. RE: Paraffin Block trimming (McMahon, Loralee A) 14. Paraffin Block triming (Paula Pierce) 15. RE: Paraffin Block triming (Bartlett, Jeanine (CDC/CCID/NCZVED)) 16. Re: Paraffin Block trimming (Kelly Boyd) 17. Re: Paraffin Block trimming (godsgalnow@aol.com) 18. RE: Paraffin Block trimming (Podawiltz, Thomas) 19. RE: Paraffin Block trimming (Martin, Gary) 20. FW: [Histonet] RE: Automating muscle and neuro stains (Ian Montgomery) 21. Sanderson's RBS Image (Suzanne Bruce) 22. RE: Paraffin Block trimming (Merced Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Mar 2009 10:09:40 -0700 From: Patty Dunlop Subject: [Histonet] equipment purchasing To: histonet@lists.utsouthwestern.edu Message-ID: <80ab7bc60903181009o6e178432m42613f7e5cc0eb18@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello, Our histo lab is currently looking into buying one or all of the following: cassette labeler, automated stainer (that can also accommodate a few special stains), and automated coverslipper (that uses minimal xylene or no xylene). Can anyone recommend good brands and models that they like as well as what vendors might have good prices? We are also looking into getting the stainer and coverslipper either used or refurbished to cut down on costs. Our lab is small and we do only about 40-50 slides per day on average. We would prefer to have the equipment to be as small as possible. Any suggestions are welcome! Thanks, Patty ------------------------------ Message: 2 Date: Wed, 18 Mar 2009 13:18:23 -0600 From: "Neil M. Fournier" Subject: [Histonet] glycerol buffered antifade media To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Everyone, I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol (pH to ~ 10.0 with NaOH) However, I notice that solution became quite a dark yellow when dissolving. Is this normal? It seems to occur every time I try to make it up. Thanks in advance Neil E-mail message checked by Spyware Doctor (6.0.0.386) Database version: 5.11990 http://www.pctools.com/en/spyware-doctor-antivirus/ ------------------------------ Message: 3 Date: Wed, 18 Mar 2009 14:20:34 -0500 From: "Margaryan, Naira" Subject: [Histonet] Double-stain for immune cell markers To: Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601E6CC03@CMHEXC01EVS.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira ------------------------------ Message: 4 Date: Wed, 18 Mar 2009 22:19:45 -0600 From: "Scott" Subject: [Histonet] Paraffin Block triming To: Message-ID: <550661ED746D44B48C04DB86EB971C75@LesliePC> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ------------------------------ Message: 5 Date: Wed, 18 Mar 2009 21:26:37 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Paraffin Block trimming To: "Scott" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 19 Mar 2009 06:34:38 -0400 (EDT) From: Pamela Marcum Subject: Re: [Histonet] Paraffin Block triming To: Scott Cc: Histonet@lists.utsouthwestern.edu Message-ID: <1128894179.63154441237458878301.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Content-Type: text/plain; charset=utf-8 Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum ----- Original Message ----- From: "Scott" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 19 Mar 2009 08:28:44 -0400 From: Denise Piontek Subject: RE: [Histonet] RE: Automating muscle and neuro stains To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail?. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_ WL_HM_express_032009#colortheme ------------------------------ Message: 8 Date: Thu, 19 Mar 2009 09:13:46 -0400 From: "Angela Bitting" Subject: Re: [Histonet] Paraffin Block trimming To: , "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <49C20CCA.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 9 Date: Thu, 19 Mar 2009 06:25:19 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, Jennifer MacDonald , Scott , Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Message-ID: <767749.47598.qm@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You will have to time worself as I did to find out that waiting fo?the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs.?80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 19 Mar 2009 09:31:21 -0400 From: "Pamela Marcum" Subject: RE: [Histonet] Paraffin Block trimming To: "'Angela Bitting'" , , "'Jennifer MacDonald'" , "'Scott'" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <000c01c9a896$fe832c60$095a5b82@vet.upenn.edu> Content-Type: text/plain; charset="US-ASCII" Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ------------------------------ Message: 11 Date: Thu, 19 Mar 2009 09:30:34 -0400 From: Peter Carroll Subject: Re: [Histonet] Paraffin Block triming To: histonet@lists.utsouthwestern.edu Message-ID: <49C248FA.5060906@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save > any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 12 Date: Thu, 19 Mar 2009 06:37:46 -0700 From: "Karin Groeger" Subject: RE: [Histonet] Paraffin Block trimming To: "Pamela Marcum" , "Angela Bitting" , , "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-bounces@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ------------------------------ Message: 13 Date: Thu, 19 Mar 2009 09:45:46 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] Paraffin Block trimming To: , , "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C946D9@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" In my experience the paraffin block trimmer is quicker and easier on your hands than the old manual scraping method. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 3/19/2009 9:25 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 19 Mar 2009 06:56:46 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Paraffin Block triming To: Histonet Message-ID: <769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 19 Mar 2009 09:58:25 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Paraffin Block triming To: "Paula Pierce" , Histonet Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=iso-8859-1 I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 19 Mar 2009 07:03:08 -0700 (PDT) From: Kelly Boyd Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, Jennifer MacDonald , Scott , Angela Bitting , rjbuesa@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Message-ID: <32862.36509.qm@web58608.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My techs use our Para Trimmer from Thermo Scientific Shandon.?It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least?twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 3/19/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo?the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs.?80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab.? It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? Thanks, Scott Hendricksen? HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 19 Mar 2009 10:08:40 -0400 From: godsgalnow@aol.com Subject: Re: [Histonet] Paraffin Block trimming To: KGroeger@USLABS.net, pmarcum@vet.upenn.edu, akbitting@geisinger.edu, histonet-bounces@lists.utsouthwestern.edu, JMacDonald@mtsac.edu, lscott@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Message-ID: <8CB76B3572A802B-1288-30F1@webmail-md03.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I agree that this is quicker than manually---you can do 4?or 5 blocks at a time. -----Original Message----- From: Karin Groeger To: Pamela Marcum ; Angela Bitting ; histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald ; Scott Cc: Histonet@lists.utsouthwestern.edu Sent: Thu, 19 Mar 2009 9:37 am Subject: RE: [Histonet] Paraffin Block trimming They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-bounces@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anyb ody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this me ssage including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 19 Mar 2009 10:24:00 -0400 From: "Podawiltz, Thomas" Subject: RE: [Histonet] Paraffin Block trimming To: Kelly Boyd , "histonet-bounces@lists.utsouthwestern.edu" , Jennifer MacDonald , Scott , Angela Bitting , "rjbuesa@yahoo.com" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BD20@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="iso-8859-1" We have one timmer. I don't use it, I hand trim simply because I am quicker with it. The other techs use the timmer to save their hands. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhisto@yahoo.com] Sent: Thursday, March 19, 2009 10:03 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting; rjbuesa@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" , "Angela Bitting" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block Ren? J. --- On Thu, 3/19/09, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Paraffin Block trimming To: histonet-bounces@lists.utsouthwestern.edu, "Jennifer MacDonald" , "Scott" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 19 Date: Thu, 19 Mar 2009 07:37:13 -0700 From: "Martin, Gary" Subject: RE: [Histonet] Paraffin Block trimming To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , "Paula Pierce" , "Histonet" Message-ID: <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="iso-8859-1" No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 19 Mar 2009 14:38:58 -0000 From: "Ian Montgomery" Subject: FW: [Histonet] RE: Automating muscle and neuro stains To: Message-ID: <57D70B824966452FA0D788FD34277E38@IBLS.GLA.AC.UK> Content-Type: text/plain; charset="us-ascii" Have I died and gone to histologists heaven? Automate an ATPase, now there would be something special. No more crying and wailing over failed or partially successful acid reversal. I'll have to retreat to a darkened room, I'm traumatised. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. Subject: RE: [Histonet] RE: Automating muscle and neuro stains I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for HotmailR. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX _WL_HM_express_032009#colortheme____________________________________________ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 19 Mar 2009 10:55:20 -0400 From: "Suzanne Bruce" Subject: [Histonet] Sanderson's RBS Image To: "histonet" Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2855191@vpss1.VetPathServicesInc.local> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? Thanks in advance, Suzanne _______________________________ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator ------------------------------ Message: 22 Date: Thu, 19 Mar 2009 10:55:36 -0400 From: Merced Leiker Subject: RE: [Histonet] Paraffin Block trimming To: "Martin, Gary" , "Bartlett, Jeanine (CDC/CCID/NCZVED)" , Paula Pierce , Histonet Message-ID: <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" wrote: > No you're not the only one ... I was wondering the same thing ... why > all the scraping. It seems to me that clean embedding does the trick > with a few exceptions. G > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 > 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to > scrape a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape > from the blocks. ;) > > But, I?do scrape using the handle end of?the same forceps I use to > pick up the ribbon and tease the sections. No sharp edge. No > electricity. > > PKP > > > ? > > > > ________________________________ > From: Peter Carroll > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I > find that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save >> any time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen? HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 64, Issue 33 **************************************** This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Mar 19 10:16:22 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Mar 19 10:20:57 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu> <769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local><1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> Message-ID: <793471102-1237476050-cardhu_decombobulator_blackberry.rim.net-1735884627-@bxe1028.bisx.prod.on.blackberry> I don't normally embed or cut my own sections anymore, but when I was doing it a few years ago on a larger scale (but still in a research setting), clean embedding generally did the trick. But when I did need to remove the excess wax on the sides of the cassette, I would melt it using a hotplate. In fact I nearly always did this during the embedding process. Once the blocks had solidified I would go back and melt the wax on the sides using the hot plate of the embedding station. Only if needed, on an individual block basis, would I scrape with a straight edge blade at the time of sectioning. -----Original Message----- From: Merced Leiker Date: Thu, 19 Mar 2009 10:55:36 To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" wrote: > No you're not the only one ... I was wondering the same thing ... why all > the scraping. It seems to me that clean embedding does the trick with a > few exceptions. G > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to scrape > a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape from > the blocks. ;) > > But, I?do scrape using the handle end of?the same forceps I use to pick > up the ribbon and tease the sections. No sharp edge. No electricity. > > PKP > > > ? > > > >________________________________ > From: Peter Carroll > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I find > that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >> time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen? HT (ASCP) >>_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ? > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From serafin <@t> HHSC.CA Thu Mar 19 10:34:28 2009 From: serafin <@t> HHSC.CA (Serafin Cristina) Date: Thu Mar 19 10:34:32 2009 Subject: [Histonet] RE: Conversion of histo lab to Lean Sigma In-Reply-To: <19740416153608.CF75864C85C0AD6F@delta.hhsc.ca> Message-ID: <50B1373A6FC8774CBE5ECDD00D0D824001B215A9@ipemail04.hhsc.ca> Hi histo-netters, Our lab is trying to go Lean and I was wondering if anyone out there has done the conversion yet? Do you have any pointers or suggestions on how to make the transition easier. Our Department is in operation from 7am to 6:30pm. Thanks and have a great day This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From ree3 <@t> leicester.ac.uk Thu Mar 19 10:37:04 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Mar 19 10:37:10 2009 Subject: [Histonet] tissue processors/UK Message-ID: <7722595275A4DD4FA225B92CDBF174A17455BB4EAE@EXC-MBX3.cfs.le.ac.uk> Best low volume tissue processor, microwave or conventional, mainly lung biopsies in the short term, any ideas chaps??, thanks. Cheers Richard Edwards Leicester University U.K. P.S. happy to hear from vendors. From jqb7 <@t> cdc.gov Thu Mar 19 10:44:36 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 19 10:45:02 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov> Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcampbell <@t> vdxpathology.com Thu Mar 19 10:45:23 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Thu Mar 19 10:45:33 2009 Subject: [Histonet] dako autostainers Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF81DF4FA@VDXSERVER01.vdxpathology.local> We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. Jen C. From jqb7 <@t> cdc.gov Thu Mar 19 10:45:28 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 19 10:46:49 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <96B125D72FDDF2F702DA3E43@bchwxp2702.ad.med.buffalo.edu> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E3@LTA3VS011.ees.hhs.gov> <96B125D72FDDF2F702DA3E43@bchwxp2702.ad.med.buffalo.edu> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E7@LTA3VS011.ees.hhs.gov> We have used a variety of cassettes and paraffin over the years...same result. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Merced Leiker [mailto:leiker@buffalo.edu] Sent: Thursday, March 19, 2009 11:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: > Don't know what to say.........I fill mine up and as long as I don't get > to rough with it (bump it or something where it sloshes) I don't have any > excess paraffin around the edges. I use metal base molds. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Merced Leiker [mailto:leiker@buffalo.edu] > Sent: Thursday, March 19, 2009 10:56 AM > To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; > Histonet Subject: RE: [Histonet] Paraffin Block trimming > > Now I'm interested in how you embed so as to not have to scrape...if i > don't add enough wax (enough to end up rising around the edges of the > cassette, hence the scraping later), i don't get a secure hold of the > block to the cassette... > > ML > > --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" > wrote: > >> No you're not the only one ... I was wondering the same thing ... why all >> the scraping. It seems to me that clean embedding does the trick with a >> few exceptions. G >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM >> To: Paula Pierce; Histonet >> Subject: RE: [Histonet] Paraffin Block triming >> >> I was wondering if I was the only one out there that rarely has to scrape >> a block. >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >> Pierce Sent: Thursday, March 19, 2009 9:57 AM >> To: Histonet >> Subject: [Histonet] Paraffin Block triming >> >> I try to embed so as to have a minimal amount of paraffin to scrape from >> the blocks. ;) >> >> But, I?do scrape using the handle end of?the same forceps I use to pick >> up the ribbon and tease the sections. No sharp edge. No electricity. >> >> PKP >> >> >> ? >> >> >> >> ________________________________ >> From: Peter Carroll >> To: histonet@lists.utsouthwestern.edu >> Sent: Thursday, March 19, 2009 8:30:34 AM >> Subject: Re: [Histonet] Paraffin Block triming >> >>> Does anybody use a paraffin block dewaxer ? >> >> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find >> that it's not only very quick, but quite accurate... >> >> >> >> Scott wrote: >>> Hi, >>> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >>> time, how well does it work? >>> >>> Thanks, >>> >>> Scott Hendricksen? HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> ? >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From algranth <@t> u.arizona.edu Thu Mar 19 10:51:01 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Mar 19 10:50:01 2009 Subject: [Histonet] processing v-e-r-y tiny samples Message-ID: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From carrolpb <@t> umdnj.edu Thu Mar 19 10:56:41 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Mar 19 10:57:05 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Message-ID: <49C26B39.8020502@umdnj.edu> i use those tiny mesh inserts for small biopsies, or in a pinch, i just wrap the tissue in a square of lint-free lens-cleaning paper... Andrea Grantham wrote: > Good Morning! > In keeping with the weirdness of the projects I get in this lab today > my question is about processing mosquito GI tracts. > I have a processing schedule - that is not the problem. I'm wondering > if anybody out in histoland has a suggestion for what kind of cassette > to use. I was thinking of the histoscreen cassette because these GI > tracts are so thin (I think thinner than a hair)and I don't want to > wrap them or use sponges because I'm afraid that I'll loose them or > crush them. > Any ideas? > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Shakun.Aswani <@t> acologix.com Thu Mar 19 11:02:50 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Thu Mar 19 11:02:54 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov> Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu Mar 19 11:06:24 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Mar 19 11:06:31 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E7@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC> <49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov> <6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local> <1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E3@LTA3VS011.ees.hhs.gov> <96B125D72FDDF2F702DA3E43@bchwxp2702.ad.med.buffalo.edu> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E7@LTA3VS011.ees.hhs.gov> Message-ID: <4BD853A8A206B35B3566FC91@bchwxp2702.ad.med.buffalo.edu> I guess one has to go to histology school to learn how to do it, then (sent under separate cover from another histonetter...) I 'm too researchy. ;-) --On Thursday, March 19, 2009 11:45 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: > We have used a variety of cassettes and paraffin over the years...same > result. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Merced Leiker [mailto:leiker@buffalo.edu] > Sent: Thursday, March 19, 2009 11:16 AM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; > Histonet Subject: RE: [Histonet] Paraffin Block trimming > > hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base > Molds...maybe it's the cassette design or type of wax used? (VWR > 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics > Biopsy Cassettes) > > > --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine > (CDC/CCID/NCZVED)" wrote: > >> Don't know what to say.........I fill mine up and as long as I don't get >> to rough with it (bump it or something where it sloshes) I don't have any >> excess paraffin around the edges. I use metal base molds. >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: Merced Leiker [mailto:leiker@buffalo.edu] >> Sent: Thursday, March 19, 2009 10:56 AM >> To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; >> Histonet Subject: RE: [Histonet] Paraffin Block trimming >> >> Now I'm interested in how you embed so as to not have to scrape...if i >> don't add enough wax (enough to end up rising around the edges of the >> cassette, hence the scraping later), i don't get a secure hold of the >> block to the cassette... >> >> ML >> >> --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" >> wrote: >> >>> No you're not the only one ... I was wondering the same thing ... why >>> all the scraping. It seems to me that clean embedding does the trick >>> with a few exceptions. G >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>> Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 >>> AM To: Paula Pierce; Histonet >>> Subject: RE: [Histonet] Paraffin Block triming >>> >>> I was wondering if I was the only one out there that rarely has to >>> scrape a block. >>> >>> >>> Jeanine Bartlett >>> Infectious Diseases Pathology Branch >>> (404) 639-3590 >>> jeanine.bartlett@cdc.hhs.gov >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >>> Pierce Sent: Thursday, March 19, 2009 9:57 AM >>> To: Histonet >>> Subject: [Histonet] Paraffin Block triming >>> >>> I try to embed so as to have a minimal amount of paraffin to scrape from >>> the blocks. ;) >>> >>> But, I?do scrape using the handle end of?the same forceps I use to pick >>> up the ribbon and tease the sections. No sharp edge. No electricity. >>> >>> PKP >>> >>> >>> ? >>> >>> >>> >>> ________________________________ >>> From: Peter Carroll >>> To: histonet@lists.utsouthwestern.edu >>> Sent: Thursday, March 19, 2009 8:30:34 AM >>> Subject: Re: [Histonet] Paraffin Block triming >>> >>>> Does anybody use a paraffin block dewaxer ? >>> >>> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find >>> that it's not only very quick, but quite accurate... >>> >>> >>> >>> Scott wrote: >>>> Hi, >>>> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >>>> time, how well does it work? >>>> >>>> Thanks, >>>> >>>> Scott Hendricksen? HT (ASCP) >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> >>>> ? >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician II >> 354 Biomedical Research Building >> School of Medicine and Biomedical Sciences >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 >> Ph: (716) 829-6033 >> Fx: (716) 829-2725 >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From algranth <@t> u.arizona.edu Thu Mar 19 11:15:57 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Mar 19 11:14:54 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.ari zona.edu> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Message-ID: <6.2.3.4.1.20090319091350.02723530@algranth.inbox.email.arizona.edu> Jeff - This is not an early April Fools joke. I don't want to wrap them so that they don't get really flattened out. I'm thinking to put them in histogel or agar now to make the orientation easier when embedding. Good idea? Andi At 08:51 AM 3/19/2009, Andrea Grantham wrote: >Good Morning! >In keeping with the weirdness of the projects I get in this lab >today my question is about processing mosquito GI tracts. >I have a processing schedule - that is not the problem. I'm >wondering if anybody out in histoland has a suggestion for what kind >of cassette to use. I was thinking of the histoscreen cassette >because these GI tracts are so thin (I think thinner than a hair)and >I don't want to wrap them or use sponges because I'm afraid that >I'll loose them or crush them. >Any ideas? > >Andi >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From foreightl <@t> gmail.com Thu Mar 19 11:14:56 2009 From: foreightl <@t> gmail.com (Pat Laurie) Date: Thu Mar 19 11:15:00 2009 Subject: [Histonet] Xmatrx (Rx) Message-ID: Histonet, Has anyone heard of this machine? We recently had a BioGenex sales person come and introduce it to us. I never knew that it existed, I?ve primarily used biogenex for antibodies. The literature looks interesting, it potentially can do IHC, CISH, SS, FISH, TUNEL on FFPE, Cellular prep, cDNA microarrays, PCR etc. It almost looks like an open version of the Ventana, with a couple of differences. Are there any labs using it? Any that would be willing to share their impressions? Thanks for your time -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From algranth <@t> u.arizona.edu Thu Mar 19 11:25:12 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Mar 19 11:24:11 2009 Subject: [Histonet] PSLIM slide printer Message-ID: <6.2.3.4.1.20090319091718.02751970@algranth.inbox.email.arizona.edu> I think mine was probably the last lab to purchase a PSLIM from AccuPlace before they sold off the printer to Fisher. Just in time! For what it is worth, I do like the printer. It is great for a small lab and is saving what is left of my wrist. However, we are on the second machine. The first one wasted about as many slides as I accepted. We called tech support and tried their suggestions but nothing worked. After we sent them a picture of what the slides looked like they called with an RMA number and sent out a replacement that is working perfectly. I'm happy! We had an option of purchasing a service/replacement agreement with the printer and I'm glad we did. I don't know if Fisher is offering this option but if you are considering the printer it might be a good idea. Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From LSebree <@t> uwhealth.org Thu Mar 19 11:33:14 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Mar 19 11:33:28 2009 Subject: [Histonet] Seeking reference lab for Langerin glycoprotein IHC staining Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF1C@UWHC-MAIL01.uwhis.hosp.wisc.edu> One of our pathologists is looking for a reference lab to perform Langerin Glycoprotein IHC staining on FFPE human tissue. Any and all responses are welcome. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From Doug.Showers <@t> propath.com Thu Mar 19 11:37:04 2009 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Thu Mar 19 11:38:35 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov> <777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> Message-ID: <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Mar 19 11:38:51 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Mar 19 11:39:06 2009 Subject: [Histonet] RE: Sandersons rapid bone stain photo Message-ID: <000401c9a8b1$30e43630$92aca290$@callis@bresnan.net> On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaining with fuchsin counterstain. The other is light or fast green and since we never used that, I can't comment on the color contrast with bone and soft tissue components. This may be the stain used in photograph??? It always is a good idea to look at the stain while performing the actual staining method. After prescribed or desired time in RBS, simply rinse quickly with running hot tap water, blot and examine under the microscope to see depth of stain for soft tissue/bone components. To view a dry section, simply place a coverglass on top of section for higher power examination. If components stained by RBS not dark enough, you can return to the hot RBS. Rinse then blot, and counterstain after that. It does not hurt the bone section to dry. In fact we preferred a dry section when going back into either stain just to prevent dilution of the stain solution, particularly when doing hand ground thick section surface staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 From b-frederick <@t> northwestern.edu Thu Mar 19 11:52:16 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 19 11:52:27 2009 Subject: [Histonet] RE: Sandersons rapid bone stain photo In-Reply-To: <000401c9a8b1$30e43630$92aca290$@callis@bresnan.net> Message-ID: Maybe the NSH hard tissue committee has a picture. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, March 19, 2009 11:39 AM To: 'Histonet' Subject: [Histonet] RE: Sandersons rapid bone stain photo On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaining with fuchsin counterstain. The other is light or fast green and since we never used that, I can't comment on the color contrast with bone and soft tissue components. This may be the stain used in photograph??? It always is a good idea to look at the stain while performing the actual staining method. After prescribed or desired time in RBS, simply rinse quickly with running hot tap water, blot and examine under the microscope to see depth of stain for soft tissue/bone components. To view a dry section, simply place a coverglass on top of section for higher power examination. If components stained by RBS not dark enough, you can return to the hot RBS. Rinse then blot, and counterstain after that. It does not hurt the bone section to dry. In fact we preferred a dry section when going back into either stain just to prevent dilution of the stain solution, particularly when doing hand ground thick section surface staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Mar 19 11:53:15 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Mar 19 11:53:29 2009 Subject: [Histonet] RE: Paraffin block trimming device - the cheap method Message-ID: <000901c9a8b3$33cbb3d0$9b631b70$@callis@bresnan.net> We coveted the ParaTrimmer for years, but could not afford to buy one. We simply use a smaller size laboratory metal hot plate, propped it into a metal pan so the hot plate is at an angle suitable to allow the melted paraffin to run onto paper towels in the pan. This is one hot plate we donate to trashing by melted paraffin, and the cost is minimal compared to the Paratrimmer. Just make sure the hot plate does not tip over and set the temperature to not burn fingers but still melt paraffin. We now "melt trim" blocks (front, sides and top) if excess paraffin exists prior to facing blocks on microtome. This has saved fingers from severe cuts particularly after one tech cut his finger with a razor blade. After many years of microtomy, trimming paraffin from blocks using dull solid scalpel blades became a difficult and painful task for old, stiff, sore finger joints. If one can trim blocks with a blade of some type, that works but eventually one ends up with osteoarthritic finger joints like me and the need for something efficient and fast. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 From JWeems <@t> sjha.org Thu Mar 19 12:01:10 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 19 12:03:24 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53EA668@ITSSSXM01V6.one.ads.che.org> Exactly right... And you can melt your blocks on the embedding center. The Thermo Paratrimmer is great tho. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 12:37 PM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From hymclab.hymclab <@t> ministryhealth.org Thu Mar 19 12:02:43 2009 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Mar 19 12:04:53 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov> <777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> Message-ID: I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From jqb7 <@t> cdc.gov Thu Mar 19 12:06:37 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 19 12:06:49 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov> <777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03EE@LTA3VS011.ees.hhs.gov> I also use mine warm........ Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, March 19, 2009 1:03 PM To: 'Doug Showers'; Histonet Subject: RE: [Histonet] Paraffin Block triming I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Mar 19 12:09:24 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Mar 19 12:09:29 2009 Subject: [Histonet] PSLIM slide printer In-Reply-To: <6.2.3.4.1.20090319091718.02751970@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20090319091718.02751970@algranth.inbox.email.arizona.edu> Message-ID: <49C27C44.4000006@pathology.washington.edu> Andi, How are you utilizing the printer? We are trying to incorporate the printer with real time printing as the tech scans the bar coded cassette. We have had one tech working with the PSLIM and they have printed about 200 slides over a couple of days, but it has been getting progressively worse in performance.If there is more than one slide needed for the block then the PSLIM can hang. It's as if it doesn't have enough memory. We are on our second or third unit and are about to give up and go to labels. The PSLIM was questionable at $5K, but out of the question at Fisher's $10+K pricing. The print quality is so much better than the Leica. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Andrea Grantham wrote: > I think mine was probably the last lab to purchase a PSLIM from > AccuPlace before they sold off the printer to Fisher. Just in time! > For what it is worth, I do like the printer. It is great for a small > lab and is saving what is left of my wrist. However, we are on the > second machine. The first one wasted about as many slides as I > accepted. We called tech support and tried their suggestions but > nothing worked. After we sent them a picture of what the slides looked > like they called with an RMA number and sent out a replacement that is > working perfectly. > I'm happy! > We had an option of purchasing a service/replacement agreement with > the printer and I'm glad we did. I don't know if Fisher is offering > this option but if you are considering the printer it might be a good > idea. > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Mar 19 12:24:09 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 19 12:24:14 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03EE@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com><82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03EE@LTA3VS011.ees.hhs.gov> Message-ID: <002901c9a8b7$83ce59b0$095a5b82@vet.upenn.edu> I think we all develop our own methods based on what we were taught and what becomes easiest or best for us. I do agree with Gayle as the fingers get older the use of these new devices is better and easier on us. We often build blocks here as a research lab with large pieces of tissue or decal bone and very large molds or sometimes embedding "L"s. Trimming can be a big job and get dangerous. I have come to love my Para Trimmer and the ease of use for trimming and sometimes forming these blocks prior to sectioning. I do love cleaning multiple blocks at a time to save time when I have average cassettes. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 1:07 PM To: hymclab; Doug Showers; Histonet Subject: RE: [Histonet] Paraffin Block triming I also use mine warm........ Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, March 19, 2009 1:03 PM To: 'Doug Showers'; Histonet Subject: RE: [Histonet] Paraffin Block triming I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Thu Mar 19 12:42:50 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Mar 19 12:42:11 2009 Subject: [Histonet] Marilyn A Weiss is out of the office as of 10 a.m. 3/19/2008 Message-ID: I will be out of the office starting 03/19/2009 and will not return until 03/24/2009. I will respond to your message when I return. From janderson <@t> halozyme.com Thu Mar 19 13:01:05 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Thu Mar 19 13:01:15 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03EE@LTA3VS011.ees.hhs.gov> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com><82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> <9A16CB5D55FC1648ADF11B63E72A1BE1BF03EE@LTA3VS011.ees.hhs.gov> Message-ID: I think it depends on whether you place your cassette bottom side down (slotted side close to the tissue) or bottom side up (open side close to the tissue) when embedding. The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 10:07 AM To: hymclab; Doug Showers; Histonet Subject: RE: [Histonet] Paraffin Block triming I also use mine warm........ Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, March 19, 2009 1:03 PM To: 'Doug Showers'; Histonet Subject: RE: [Histonet] Paraffin Block triming I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From serafin <@t> HHSC.CA Thu Mar 19 13:02:51 2009 From: serafin <@t> HHSC.CA (Serafin Cristina) Date: Thu Mar 19 13:03:00 2009 Subject: [Histonet] RE: Paraffin Block Trimming In-Reply-To: <19740413054056.31CF874DC5BEC0A9@gamma.hhsc.ca> Message-ID: <50B1373A6FC8774CBE5ECDD00D0D824001B215AA@ipemail04.hhsc.ca> We use a paraffin block dewaxer in our lab. It works great. I find it saves time and doesn't create as much of a mess (with wax shavings). Some people in our lab still prefer to trim the excess wax with a blade or forceps. I guess it's all personal preference. ---------------------------------------------------------------------- Message: 1 Date: Thu, 19 Mar 2009 15:16:22 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] Paraffin Block trimming To: "Merced Leiker" , "Martin, Gary" , "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Paula Pierce" , "Histonet" Message-ID: <793471102-1237476050-cardhu_decombobulator_blackberry.rim.net-1735884627-@bxe1028.bisx.prod.on.blackberry> Content-Type: text/plain; charset="Windows-1252" I don't normally embed or cut my own sections anymore, but when I was doing it a few years ago on a larger scale (but still in a research setting), clean embedding generally did the trick. But when I did need to remove the excess wax on the sides of the cassette, I would melt it using a hotplate. In fact I nearly always did this during the embedding process. Once the blocks had solidified I would go back and melt the wax on the sides using the hot plate of the embedding station. Only if needed, on an individual block basis, would I scrape with a straight edge blade at the time of sectioning. -----Original Message----- From: Merced Leiker Date: Thu, 19 Mar 2009 10:55:36 To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" wrote: > No you're not the only one ... I was wondering the same thing ... why all > the scraping. It seems to me that clean embedding does the trick with a > few exceptions. G > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to scrape > a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape from > the blocks. ;) > > But, I?do scrape using the handle end of?the same forceps I use to pick > up the ribbon and tease the sections. No sharp edge. No electricity. > > PKP > > > ? > > > >________________________________ > From: Peter Carroll > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I find > that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >> time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen? HT (ASCP) >>_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ? > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 19 Mar 2009 08:45:23 -0700 From: "Jennifer Campbell" Subject: [Histonet] dako autostainers To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF81DF4FA@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. Jen C. ------------------------------ Message: 6 Date: Thu, 19 Mar 2009 11:45:28 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Paraffin Block trimming To: "Merced Leiker" , "Martin, Gary" , "Paula Pierce" , Histonet Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03E7@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=iso-8859-1 We have used a variety of cassettes and paraffin over the years...same result. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Merced Leiker [mailto:leiker@buffalo.edu] Sent: Thursday, March 19, 2009 11:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: > Don't know what to say.........I fill mine up and as long as I don't get > to rough with it (bump it or something where it sloshes) I don't have any > excess paraffin around the edges. I use metal base molds. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Merced Leiker [mailto:leiker@buffalo.edu] > Sent: Thursday, March 19, 2009 10:56 AM > To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; > Histonet Subject: RE: [Histonet] Paraffin Block trimming > > Now I'm interested in how you embed so as to not have to scrape...if i > don't add enough wax (enough to end up rising around the edges of the > cassette, hence the scraping later), i don't get a secure hold of the > block to the cassette... > > ML > > --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" > wrote: > >> No you're not the only one ... I was wondering the same thing ... why all >> the scraping. It seems to me that clean embedding does the trick with a >> few exceptions. G >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM >> To: Paula Pierce; Histonet >> Subject: RE: [Histonet] Paraffin Block triming >> >> I was wondering if I was the only one out there that rarely has to scrape >> a block. >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >> Pierce Sent: Thursday, March 19, 2009 9:57 AM >> To: Histonet >> Subject: [Histonet] Paraffin Block triming >> >> I try to embed so as to have a minimal amount of paraffin to scrape from >> the blocks. ;) >> >> But, I?do scrape using the handle end of?the same forceps I use to pick >> up the ribbon and tease the sections. No sharp edge. No electricity. >> >> PKP >> >> >> ? >> >> >> >> ________________________________ >> From: Peter Carroll >> To: histonet@lists.utsouthwestern.edu >> Sent: Thursday, March 19, 2009 8:30:34 AM >> Subject: Re: [Histonet] Paraffin Block triming >> >>> Does anybody use a paraffin block dewaxer ? >> >> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find >> that it's not only very quick, but quite accurate... >> >> >> >> Scott wrote: >>> Hi, >>> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >>> time, how well does it work? >>> >>> Thanks, >>> >>> Scott Hendricksen? HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> ? >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 7 Date: Thu, 19 Mar 2009 08:51:01 -0700 From: Andrea Grantham Subject: [Histonet] processing v-e-r-y tiny samples To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 8 Date: Thu, 19 Mar 2009 11:56:41 -0400 From: Peter Carroll Subject: Re: [Histonet] processing v-e-r-y tiny samples To: Andrea Grantham Cc: histonet@lists.utsouthwestern.edu Message-ID: <49C26B39.8020502@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed i use those tiny mesh inserts for small biopsies, or in a pinch, i just wrap the tissue in a square of lint-free lens-cleaning paper... Andrea Grantham wrote: > Good Morning! > In keeping with the weirdness of the projects I get in this lab today > my question is about processing mosquito GI tracts. > I have a processing schedule - that is not the problem. I'm wondering > if anybody out in histoland has a suggestion for what kind of cassette > to use. I was thinking of the histoscreen cassette because these GI > tracts are so thin (I think thinner than a hair)and I don't want to > wrap them or use sponges because I'm afraid that I'll loose them or > crush them. > Any ideas? > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 9 Date: Thu, 19 Mar 2009 09:02:50 -0700 From: "Shakun Aswani" Subject: RE: [Histonet] Paraffin Block triming To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Paula Pierce" , "Histonet" Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com> Content-Type: text/plain; charset="iso-8859-1" Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 19 Mar 2009 12:06:24 -0400 From: Merced Leiker Subject: RE: [Histonet] Paraffin Block trimming To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Martin, Gary" , Paula Pierce , Histonet Message-ID: <4BD853A8A206B35B3566FC91@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed I guess one has to go to histology school to learn how to do it, then (sent under separate cover from another histonetter...) I 'm too researchy. ;-) --On Thursday, March 19, 2009 11:45 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: > We have used a variety of cassettes and paraffin over the years...same > result. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: Merced Leiker [mailto:leiker@buffalo.edu] > Sent: Thursday, March 19, 2009 11:16 AM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; > Histonet Subject: RE: [Histonet] Paraffin Block trimming > > hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base > Molds...maybe it's the cassette design or type of wax used? (VWR > 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics > Biopsy Cassettes) > > > --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine > (CDC/CCID/NCZVED)" wrote: > >> Don't know what to say.........I fill mine up and as long as I don't get >> to rough with it (bump it or something where it sloshes) I don't have any >> excess paraffin around the edges. I use metal base molds. >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: Merced Leiker [mailto:leiker@buffalo.edu] >> Sent: Thursday, March 19, 2009 10:56 AM >> To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; >> Histonet Subject: RE: [Histonet] Paraffin Block trimming >> >> Now I'm interested in how you embed so as to not have to scrape...if i >> don't add enough wax (enough to end up rising around the edges of the >> cassette, hence the scraping later), i don't get a secure hold of the >> block to the cassette... >> >> ML >> >> --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" >> wrote: >> >>> No you're not the only one ... I was wondering the same thing ... why >>> all the scraping. It seems to me that clean embedding does the trick >>> with a few exceptions. G >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>> Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 >>> AM To: Paula Pierce; Histonet >>> Subject: RE: [Histonet] Paraffin Block triming >>> >>> I was wondering if I was the only one out there that rarely has to >>> scrape a block. >>> >>> >>> Jeanine Bartlett >>> Infectious Diseases Pathology Branch >>> (404) 639-3590 >>> jeanine.bartlett@cdc.hhs.gov >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >>> Pierce Sent: Thursday, March 19, 2009 9:57 AM >>> To: Histonet >>> Subject: [Histonet] Paraffin Block triming >>> >>> I try to embed so as to have a minimal amount of paraffin to scrape from >>> the blocks. ;) >>> >>> But, I?do scrape using the handle end of?the same forceps I use to pick >>> up the ribbon and tease the sections. No sharp edge. No electricity. >>> >>> PKP >>> >>> >>> ? >>> >>> >>> >>> ________________________________ >>> From: Peter Carroll >>> To: histonet@lists.utsouthwestern.edu >>> Sent: Thursday, March 19, 2009 8:30:34 AM >>> Subject: Re: [Histonet] Paraffin Block triming >>> >>>> Does anybody use a paraffin block dewaxer ? >>> >>> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find >>> that it's not only very quick, but quite accurate... >>> >>> >>> >>> Scott wrote: >>>> Hi, >>>> ? ? Does anybody use a paraffin block dewaxer ?? If so does it save any >>>> time, how well does it work? >>>> >>>> Thanks, >>>> >>>> Scott Hendricksen? HT (ASCP) >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> >>>> ? >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> Merced M Leiker >> Research Technician II >> 354 Biomedical Research Building >> School of Medicine and Biomedical Sciences >> State University of New York at Buffalo >> 3435 Main St, Buffalo, NY 14214 >> Ph: (716) 829-6033 >> Fx: (716) 829-2725 >> >> No trees were harmed in the sending of this email. >> However, many electrons were severely inconvenienced. >> >> >> >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 11 Date: Thu, 19 Mar 2009 09:15:57 -0700 From: Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.1.20090319091350.02723530@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Jeff - This is not an early April Fools joke. I don't want to wrap them so that they don't get really flattened out. I'm thinking to put them in histogel or agar now to make the orientation easier when embedding. Good idea? Andi At 08:51 AM 3/19/2009, Andrea Grantham wrote: >Good Morning! >In keeping with the weirdness of the projects I get in this lab >today my question is about processing mosquito GI tracts. >I have a processing schedule - that is not the problem. I'm >wondering if anybody out in histoland has a suggestion for what kind >of cassette to use. I was thinking of the histoscreen cassette >because these GI tracts are so thin (I think thinner than a hair)and >I don't want to wrap them or use sponges because I'm afraid that >I'll loose them or crush them. >Any ideas? > >Andi >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 12 Date: Thu, 19 Mar 2009 09:14:56 -0700 From: Pat Laurie Subject: [Histonet] Xmatrx (Rx) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Histonet, Has anyone heard of this machine? We recently had a BioGenex sales person come and introduce it to us. I never knew that it existed, I've primarily used biogenex for antibodies. The literature looks interesting, it potentially can do IHC, CISH, SS, FISH, TUNEL on FFPE, Cellular prep, cDNA microarrays, PCR etc. It almost looks like an open version of the Ventana, with a couple of differences. Are there any labs using it? Any that would be willing to share their impressions? Thanks for your time -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com ------------------------------ Message: 13 Date: Thu, 19 Mar 2009 09:25:12 -0700 From: Andrea Grantham Subject: [Histonet] PSLIM slide printer To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.1.20090319091718.02751970@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I think mine was probably the last lab to purchase a PSLIM from AccuPlace before they sold off the printer to Fisher. Just in time! For what it is worth, I do like the printer. It is great for a small lab and is saving what is left of my wrist. However, we are on the second machine. The first one wasted about as many slides as I accepted. We called tech support and tried their suggestions but nothing worked. After we sent them a picture of what the slides looked like they called with an RMA number and sent out a replacement that is working perfectly. I'm happy! We had an option of purchasing a service/replacement agreement with the printer and I'm glad we did. I don't know if Fisher is offering this option but if you are considering the printer it might be a good idea. Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 14 Date: Thu, 19 Mar 2009 11:33:14 -0500 From: "Sebree Linda A" Subject: [Histonet] Seeking reference lab for Langerin glycoprotein IHC staining To: "Histonet" Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF1C@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" One of our pathologists is looking for a reference lab to perform Langerin Glycoprotein IHC staining on FFPE human tissue. Any and all responses are welcome. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 15 Date: Thu, 19 Mar 2009 11:37:04 -0500 From: "Doug Showers" Subject: RE: [Histonet] Paraffin Block triming To: "Histonet" Message-ID: <82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> Content-Type: text/plain; charset="iso-8859-1" I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:Shakun.Aswani@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I?do scrape using the handle end of?the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ? ________________________________ From: Peter Carroll To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, >? ? Does anybody use a paraffin block dewaxer ?? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen? HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >? This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From sbreeden <@t> nmda.nmsu.edu Thu Mar 19 13:09:21 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Mar 19 13:09:28 2009 Subject: [Histonet] Mosquito GI Tract Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6765@nmdamailsvr.nmda.ad.nmsu.edu> And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How would you even KNOW you had the darned GI tract to begin with? Are these Texas Mosquitos? I'm just sayin'... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From gu.lang <@t> gmx.at Thu Mar 19 13:39:45 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 19 13:39:52 2009 Subject: AW: [Histonet] Paraffin Block triming In-Reply-To: References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com><82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> Message-ID: <2B8369595E9548649478C8FFE145670B@dielangs.at> I think, it depends on the amount of paraffin you fill in the mold, before you put the cassette on it. If it is a little too much, you press the paraffin out. Gudrun Lang From CIngles <@t> uwhealth.org Thu Mar 19 13:38:13 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Mar 19 13:43:11 2009 Subject: [Histonet] Paraffin Block trimming References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><6ED9D4252F278841A0593D3D788AF24C04D283C8@mailsvr.MARSHMED.local><1E3C94C039A525739E0774E6@bchwxp2702.ad.med.buffalo.edu><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E3@LTA3VS011.ees.hhs.gov> <96B125D72FDDF2F702DA3E43@bchwxp2702.ad.med.buffalo.edu> Message-ID: I've noticed that if I put a little extra pressure on the cassette on top of the mold when putting in the liquid paraffin it doesn't seem to seep out the bottom sides and therefore cleaner blocks. The fill level in the cassette matters too. I usually fill mine about 3/4 to the top of the cassette to compensate for shrinkage during chilling and still have the block and cassette firmly mounted together. We use regular fisherbrand cassettes and metal molds. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Merced Leiker Sent: Thu 3/19/2009 10:15 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) From tbraud <@t> holyredeemer.com Thu Mar 19 13:44:45 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Mar 19 13:44:50 2009 Subject: [Histonet] RE:cassette labeler In-Reply-To: <01f2653b00002c1d@HolyRedeemer.com> Message-ID: Our techs and pathologists love our Leica cassette and slide printers. We've had them for over 2 years now, with few problems. The cassette labeler holds up to 6 different colors, and has an additional manual feed for anything special. The slide holds up to 3 different slide types, and also has a manual feed. Its my understanding that Sakura uses the exact same hardware. They are great time savers and very easy to use. Be sure to always order the optional "unload station". It helps keep order. We are looking forward to interfacing the slide printer with CoPath next year. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3874 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From jqb7 <@t> cdc.gov Thu Mar 19 13:47:43 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 19 13:48:02 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <2B8369595E9548649478C8FFE145670B@dielangs.at> References: <550661ED746D44B48C04DB86EB971C75@LesliePC><49C248FA.5060906@umdnj.edu><769731.2958.qm@web1107.biz.mail.sk1.yahoo.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE57E@EXCHANGE.acologix.com><9A16CB5D55FC1648ADF11B63E72A1BE1BF03E6@LTA3VS011.ees.hhs.gov><777AB0DE519C8E46A6220E2287C5BAD301ADE586@EXCHANGE.acologix.com><82C7248978CB50469FD6BA68EBBEFE67EB477E@exchange.propathlab.com> <2B8369595E9548649478C8FFE145670B@dielangs.at> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF03F6@LTA3VS011.ees.hhs.gov> I use the minimum amount in the base mold then top it off after I add the cassette top. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, March 19, 2009 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Paraffin Block triming I think, it depends on the amount of paraffin you fill in the mold, before you put the cassette on it. If it is a little too much, you press the paraffin out. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Paul <@t> Firnschild.com Thu Mar 19 13:49:40 2009 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Thu Mar 19 13:49:45 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 33 In-Reply-To: <200903191456.n2JEu6hK007004@ns-mr18.netsolmail.com> Message-ID: <005701c9a8c3$76a87390$6502a8c0@qa44c0f386def9> Hi Patty and all Histoneters, I am an independent histology equipment repair technician, based in the Atlanta, GA. Sometimes I acquire used equipment for resale and right now I have: 1) Leica Autostainer XL 1) Leica CV 5000 cover slipper Others can give testament to the quality and reliability of Leica instruments. (with the possible exception of their tissue processors). I invite you to respond either on or off of Histonet. References available upon request. Best regards, Paul Firnschild QA Support Services Inc. 404.291.3715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From dholmes <@t> anatomy.umsmed.edu Thu Mar 19 13:54:55 2009 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Thu Mar 19 13:55:16 2009 Subject: [Histonet] dehydration Message-ID: <49C24EAF02000082000367A4@GWIA1.umsmed.edu> In an emergency situation (like not enough hrs in a day) - how long can tissue remain in the final ETOH before going into the paraffin embedding stage? I do all my work manually - no automation here!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From leiker <@t> buffalo.edu Thu Mar 19 14:11:08 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Mar 19 14:11:14 2009 Subject: [Histonet] Mosquito GI Tract In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6765@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6765@nmdamailsvr.nmda.ad.nm su.edu> Message-ID: lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:09 PM -0600 "Breeden, Sara" wrote: > And here I thought I had an unusual project today, finding out as I did > that my boss is thinking of examining up to 1000 cattle for TB (at 10 > lymph nodes/beast) using AFB and asking me to cost it for him. I think > the Mosquito GI Tract Processing and Embedding question has my project > beat! How would you even KNOW you had the darned GI tract to begin > with? Are these Texas Mosquitos? I'm just sayin'... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Jackie.O'Connor <@t> abbott.com Thu Mar 19 14:27:45 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Mar 19 14:28:09 2009 Subject: [Histonet] Mosquito GI Tract In-Reply-To: Message-ID: More interesting - why is someone even looking at the mosquito GI tract? What happens in there that we need to know about? I guess for all the diseases those filthy beasts carry - they must harbor viruses and bacteria somewhere. Did you know that mosquitos did not exist in Hawaii until a little more than 100 years ago? Durn Westerners brought them in the barrels of drinking water they carried on their ships. Welcome to paradise. Oh - they also brought measles. Thanks a lot. Merced Leiker Sent by: histonet-bounces@lists.utsouthwestern.edu 03/19/2009 02:11 PM To "Breeden, Sara" , histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Mosquito GI Tract lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:09 PM -0600 "Breeden, Sara" wrote: > And here I thought I had an unusual project today, finding out as I did > that my boss is thinking of examining up to 1000 cattle for TB (at 10 > lymph nodes/beast) using AFB and asking me to cost it for him. I think > the Mosquito GI Tract Processing and Embedding question has my project > beat! How would you even KNOW you had the darned GI tract to begin > with? Are these Texas Mosquitos? I'm just sayin'... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Mar 19 14:29:52 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Mar 19 14:30:12 2009 Subject: [Histonet] Fluoro Jade Query In-Reply-To: Message-ID: What's the latest and greatest on Fluoro Jade for degenerating neurons? I've found a few references, but they are old - - so am I - just celebrated my birthday - - - - anyway - -who's got the best answer? Winner gets a Hershey Bar! Jackie From petepath <@t> yahoo.com Thu Mar 19 14:32:00 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Mar 19 14:32:05 2009 Subject: [Histonet] Re:Mosquito GI Tract Message-ID: <161183.9576.qm@web45103.mail.sp1.yahoo.com> Hi Sara, ? I?would try to wrap the tissue?placing it in the center of a piece of smooth lens paper carefully folding it so the tissue stays in place and can be easily and carfully unwrapped. Try to make a small packet not much too much larger than the tissue. If you can use a drop of eosin on the tissue?it will help see it. I would then wrap this small envelope of lens paper in a second larger piece that will fit snugly into the casette so that it will not move. Be very careful to unwrap the tissue the same way you wrapped it. Stephen Peters M.D.? 201 847 0052 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From rjbuesa <@t> yahoo.com Thu Mar 19 14:33:50 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 19 14:33:54 2009 Subject: [Histonet] dehydration In-Reply-To: <49C24EAF02000082000367A4@GWIA1.umsmed.edu> Message-ID: <681090.79424.qm@web65705.mail.ac4.yahoo.com> It seems that you are missing the "clearing" step or antemedium in the sequence you describe but, the tissue should not stay too long in neither alcohol (unless it is 2-propanol) or in the antemedium (unless it is mineral oil) in which cases the time in them is not an issue. Ren? J. --- On Thu, 3/19/09, Dianne Holmes wrote: From: Dianne Holmes Subject: [Histonet] dehydration To: "Histonet" Date: Thursday, March 19, 2009, 2:54 PM In an emergency situation (like not enough hrs in a day) - how long can tissue remain in the final ETOH before going into the paraffin embedding stage? I do all my work manually - no automation here!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbisher <@t> Princeton.EDU Thu Mar 19 14:52:16 2009 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Thu Mar 19 14:53:58 2009 Subject: [Histonet] Mosquito GI Tract In-Reply-To: Message-ID: Actually I had a project to find bacteria in the ovaries of drosophila. The bacteria looked a lot like mitochondria and about the same size. That was a lot of fun, but at least I got to use a TEM for the imaging. You could barely see the sample in the dissecting microscope and it was only after I had post fixed it with osmium tetroxide and they turned black. And they wanted longitudinal sections too! That was fun ;-| On 3/19/09 3:27 PM, "Jackie M O'Connor" wrote: > More interesting - why is someone even looking at the mosquito GI tract? > What happens in there that we need to know about? I guess for all the > diseases those filthy beasts carry - they must harbor viruses and bacteria > somewhere. Did you know that mosquitos did not exist in Hawaii until a > little more than 100 years ago? Durn Westerners brought them in the > barrels of drinking water they carried on their ships. Welcome to > paradise. Oh - they also brought measles. Thanks a lot. > > > > > > Merced Leiker > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/19/2009 02:11 PM > > To > "Breeden, Sara" , > histonet@lists.utsouthwestern.edu > cc > > Subject > Re: [Histonet] Mosquito GI Tract > > > > > > > lol...I wanted to say the same thing...and how in the world can you even > see it when you section it? How do you stain it without losing it from the > > slide? Is there really enough surface area for it to adhere and withstand > washing? ...and it's ALMOST Friday...! > > --On Thursday, March 19, 2009 12:09 PM -0600 "Breeden, Sara" > wrote: > >> And here I thought I had an unusual project today, finding out as I did >> that my boss is thinking of examining up to 1000 cattle for TB (at 10 >> lymph nodes/beast) using AFB and asking me to cost it for him. I think >> the Mosquito GI Tract Processing and Embedding question has my project >> beat! How would you even KNOW you had the darned GI tract to begin >> with? Are these Texas Mosquitos? I'm just sayin'... >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 4700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Thu Mar 19 15:32:32 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Thu Mar 19 15:33:03 2009 Subject: [Histonet] Sanderson's Rapid Bone Stain Message-ID: <3848F82A12CD4FD7BDDAD4EF243B37FE@shop1e2e996aa5> Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. From kenneth.a.troutman <@t> Vanderbilt.Edu Thu Mar 19 15:36:17 2009 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Mar 19 15:36:21 2009 Subject: [Histonet] Double-stain for immune cell markers Message-ID: <37DEF9AF72994947AF693956A59B9B66019A682B@mailbe03.mc.vanderbilt.edu> Hi Naira, Try Biocare. They make fantastic reagents for research as well as some great double-stain kits. As for the antibodies, it looks like you might have to create these either as cocktails (a lot of front-end work) or just make your stain longer (a lot of overall work) by staining seprately. Abcam has some esoteric reagents that you might be able to use for some "non-mainstream species" like horse or rat or guinea pig. Also I did not see which markers you are planning to stain for (there are quite a few "immune cell markers"). I might be able to help you find good reagents if I had that information. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Message: 3 Date: Wed, 18 Mar 2009 14:20:34 -0500 From: "Margaryan, Naira" Subject: [Histonet] Double-stain for immune cell markers To: Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601E6CC03@CMHEXC01EVS.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira From sbruce <@t> vetpathservicesinc.com Thu Mar 19 15:39:16 2009 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Thu Mar 19 15:39:39 2009 Subject: [Histonet] RE:Sanderson's RBS image Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A285519B@vpss1.VetPathServicesInc.local> Thanks Gayle, I also looked at the Surgipath image, my slide was much more brilliant then that one. I did use the acid fuchsin counter stain. I just wasn't sure if mine would be considered 'over stained.' I'll send you an image that Jack Ratliff sent me that compares to what I was seeing today. I'm not using GMA, but Technovit 7200 ~100 micron thick ground sections. Suzanne Date: Thu, 19 Mar 2009 10:38:51 -0600 From: "gayle callis" Subject: [Histonet] RE: Sandersons rapid bone stain photo To: "'Histonet'" Message-ID: <000401c9a8b1$30e43630$92aca290$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaining with fuchsin counterstain. The other is light or fast green and since we never used that, I can't comment on the color contrast with bone and soft tissue components. This may be the stain used in photograph??? It always is a good idea to look at the stain while performing the actual staining method. After prescribed or desired time in RBS, simply rinse quickly with running hot tap water, blot and examine under the microscope to see depth of stain for soft tissue/bone components. To view a dry section, simply place a coverglass on top of section for higher power examination. If components stained by RBS not dark enough, you can return to the hot RBS. Rinse then blot, and counterstain after that. It does not hurt the bone section to dry. In fact we preferred a dry section when going back into either stain just to prevent dilution of the stain solution, particularly when doing hand ground thick section surface staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 From Bryan.Watson <@t> parkview.com Thu Mar 19 15:39:14 2009 From: Bryan.Watson <@t> parkview.com (Bryan Watson) Date: Thu Mar 19 15:39:44 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Message-ID: <49C27532.5674.0085.1@parkview.com> I may never complain about tiny GI or bronch biopsies ever again! >>> Andrea Grantham 3/19/2009 11:51 AM >>> Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Mar 19 15:45:34 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Mar 19 15:45:39 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <49C27532.5674.0085.1@parkview.com> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> <49C27532.5674.0085.1@parkview.com> Message-ID: <004101c9a8d3$a6dc48b0$095a5b82@vet.upenn.edu> Andi only gets the fun stuff!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Thursday, March 19, 2009 4:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! >>> Andrea Grantham 3/19/2009 11:51 AM >>> Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Mar 19 15:51:30 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Mar 19 15:52:15 2009 Subject: [Histonet] Re: processing v-e-r-y tiny samples Message-ID: Andi, We process E7.0 mouse embryos and have problems sometimes because they are so very tiny and fragile. We've wrapped them and sometimes (but not all the time) had them break and flatten. Usually we use the histoscreen cassettes. They still will sometimes break apart using these but we have our best luck using them. You might find, though, that even with the mesh, the diameter of the holes may be big enough to let the sample pass through if the gut samples are as small as you say. We tried the cell saver mesh inserts and they were a disaster. Lost the sample in them. I won't use them for our embryo work. As for the histogel or agar, we've had difficulty using either in our paraffin processing. Recent histonet emails reveal other folks having a problem with the stuff randomly getting hard and brittle, like plastic and ruining the sample for sectioning. We may have 4 or 5 blocks, all go through the processor together, and one might have the problem of turning brittle. According to the other emails, Richard Allan folks hadn't any clue as to what was happening or how to fix it. And I'm clueless as well. Wish I knew how to consistently process things without this happening. Pre-embedding in a matrix like this would be so very helpful to allowing us to manipulate and properly orient tiny, fragile samples. Good luck, and let us know what you come up with! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From kaushik.k.shah <@t> gmail.com Thu Mar 19 15:59:30 2009 From: kaushik.k.shah <@t> gmail.com (Kaushik Shah) Date: Thu Mar 19 15:59:35 2009 Subject: [Histonet] Free- Floating Frozen Brain Sections Message-ID: <9b16f2680903191359i3cabb1e3pf6779fbb4d80e741@mail.gmail.com> Hi: I am new to this Forum and also to Immunohistochemistry of frozen sections. I want to use Floating Frozen brain sections to do some of immunohisto and immunofluorescence study. I will be grateful to you if you could provide me detail protocol for free floating brain sections. I meant fixation, cryopreservation, if want to store the tissue which cryoprotectant to be used and how to proceed further for staining and treating with antibodies. At least for now I want to use Hypoxyprobe, a marker for Hypoxia. and Vascular marker CD31 along with Neuronal and astrocyte marker. I Guess its going to be Multilabeling. Also i would like to have information on multilabeling. your suggestion on doing 4 markers at a time in one tissue. thanks -- Shah. Kaushik K From gu.lang <@t> gmx.at Thu Mar 19 16:54:31 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 19 16:54:37 2009 Subject: AW: [Histonet] Re: processing v-e-r-y tiny samples In-Reply-To: References: Message-ID: <1C33F1E20B6946B49178E78FB513823C@dielangs.at> Our cytolab produces cytoblocks with plasma, that they bring to coagulation. The coagel is a smooth droplet in the size of 5-10 mm diameter. I could imagine, that the GI can be caught in such a coagel, and then be placed in a mesh-cassette. Gudrun Lang From cathy <@t> wasatchhisto.com Thu Mar 19 17:02:46 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Thu Mar 19 17:03:10 2009 Subject: [Histonet] free Tissue Tek II vacuum infiltrator Message-ID: <2E6629EF9E3B46BB91A4CC0FF242437D@shop1e2e996aa5> I have an old Tissue Tek II vacuum infiltrator free to a good home. Only cost will be shipping via ground..........either FedEx or UPS. Cathy Mayton Wasatch Histo Consultants, Inc. From zerfasp <@t> ors.od.nih.gov Thu Mar 19 17:17:55 2009 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Thu Mar 19 17:17:59 2009 Subject: [Histonet] list of special stains Message-ID: <424D930E0319E0469DD9C61502A50E300210217A@NIHCESMLBX4.nih.gov> Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From gayle.callis <@t> bresnan.net Thu Mar 19 17:21:35 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Mar 19 17:21:48 2009 Subject: [Histonet] Sanderson's Rapid Bone Stain In-Reply-To: <3848F82A12CD4FD7BDDAD4EF243B37FE@shop1e2e996aa5> References: <3848F82A12CD4FD7BDDAD4EF243B37FE@shop1e2e996aa5> Message-ID: <000001c9a8e1$11f0c6f0$35d254d0$@callis@bresnan.net> One other counterstain can be used also. Alizarin Red S for the calcium as long as acid etching is not performed on the surface of thick sections. I would presume von Kossa could be used also if Alizarin Red S has been done in past. Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton Sent: Thursday, March 19, 2009 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Rapid Bone Stain Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Mar 19 17:28:50 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Mar 19 17:29:01 2009 Subject: [Histonet] Paraffin Block triming In-Reply-To: <1128894179.63154441237458878301.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Message-ID: We also have a paratrimmer and agree with Pam Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, 19 March 2009 9:35 PM To: Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block triming Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum ----- Original Message ----- From: "Scott" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Thu Mar 19 17:30:04 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Mar 19 17:30:10 2009 Subject: [Histonet] Paraffin Block trimming In-Reply-To: <49C20CCA.2B7F.00C9.0@geisinger.edu> Message-ID: No, We find them faster and we believe safer Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, 20 March 2009 12:14 AM To: histonet-bounces@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2009 09:24 PM To cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gayle.callis <@t> bresnan.net Thu Mar 19 17:48:18 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Mar 19 17:48:30 2009 Subject: [Histonet] van Giesons for Sanderson's Rapid Bone Stain can be Unna's modified VG In-Reply-To: <3848F82A12CD4FD7BDDAD4EF243B37FE@shop1e2e996aa5> References: <3848F82A12CD4FD7BDDAD4EF243B37FE@shop1e2e996aa5> Message-ID: <000301c9a8e4$cd786880$68693980$@callis@bresnan.net> Unna's modified van Gieson's stain for the Rapid Bone Stain is reported to give deeper colors than the VG recipe/solution commonly found in histotechnology textbooks. It contains a higher concentration of dyes but also contains nitric acid which is going to acid etch the surface of the bone a bit. The resulting counterstain will have deeper colors. Be aware that commercial solutions of van Giesons might give weaker staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton Sent: Thursday, March 19, 2009 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Rapid Bone Stain Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lscott <@t> sfcn.org Thu Mar 19 23:54:32 2009 From: lscott <@t> sfcn.org (Scott) Date: Thu Mar 19 23:54:54 2009 Subject: [Histonet] Paraffin Block triming Thanks for the info Message-ID: <91995BE6D11F4E1886FE76C48753DB73@LesliePC> Well thanks for all of the input. We have always scraped the blocks with an empty cold basemold, and it works fine. I had recieved an e-mail advertisment from one of our suppliers, it had a picture of the TBS brand Shur Trim block trimmer. I was just thinking it might save a little time, and mess. We use the Simport cassettes with a metal lid. I have found that if I dont have enough paraffin to scrape a little off the sides, it usually doesn't have enough to securly hold the specimen. Sometimes that causes sectioning issues. So I over do it a little to save myself the trouble of re-embedding. Just one of my quirks I guess. Thanks again, Scott Hendricksen HT (ASCP) From louise.renton <@t> gmail.com Fri Mar 20 02:31:22 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Mar 20 02:31:32 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Message-ID: Shandon - or whatever they're called this week- sell nylon mesh biopsy bags. These are flat bags, sealed on 3 sides. I have found that if you cut one corner off (ie, a oblong piece with 2 sides still sealed) just a tiny bit bigger than the cassette, you can open the bag, like a book, insert the sample and flatten the bag over the specimen and place it snug and flat in the bottom of the casette. It sems to stay like that through processing (esp if kept the cassette is kept flat in the basket), and is easy to locate against the mesh for embedding. On 3/19/09, Andrea Grantham wrote: > > Good Morning! > In keeping with the weirdness of the projects I get in this lab today my > question is about processing mosquito GI tracts. > I have a processing schedule - that is not the problem. I'm wondering if > anybody out in histoland has a suggestion for what kind of cassette to use. > I was thinking of the histoscreen cassette because these GI tracts are so > thin (I think thinner than a hair)and I don't want to wrap them or use > sponges because I'm afraid that I'll loose them or crush them. > Any ideas? > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From histonet.nospam <@t> vneubert.com Fri Mar 20 05:19:36 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Mar 20 05:19:39 2009 Subject: [Histonet] Rehydration // urgent Message-ID: Hi there, please help immediatly :) I want to rehydrate two specimen which have been processed as follows: 1h NBF 1h 70% 2-propanol (isopropanol) 1h 80% 2-propanol I need to go back to NBF as I want to add some more specimen but can process only one basket at one time (I'm using Microm STP120). Thanks for your replies! V. Neubert From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Mar 20 08:23:02 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Mar 20 08:23:06 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <49C27532.5674.0085.1@parkview.com> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> <49C27532.5674.0085.1@parkview.com> Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A0B58EB@mailbe01.mc.vanderbilt.edu> Hi Andi, Happy Friday! I know there's been a "Histogel backlash" of late, but I would still recommend trying it. I haven't had any problems. We use it for scant neuro specimens every day. We also have used H.G. for mouse sural nerve biopsies that needed to retain orientation (very tiny). If you do use Histogel, do not put it in a histoscreen or biopsy cassette and be sure to put it on a process that's long enough. People often use the cassette/process based on the size of the tissue, not of the Histogel block. I'd ask thermo for a sample of H.G. and try it. If you need any Histogel help, let me know. I'm a huge fan! Have a great day, Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Bryan Watson [mailto:Bryan.Watson@parkview.com] Sent: Thursday, March 19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! >>> Andrea Grantham 3/19/2009 11:51 AM >>> Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Fri Mar 20 08:59:53 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Fri Mar 20 09:02:32 2009 Subject: [Histonet] Histogel In-Reply-To: <5F3F860CFE0F4741B1D87A88A58FAE9A0B58EB@mailbe01.mc.vanderbilt.edu> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu><49C27532.5674.0085.1@parkview.com><5F3F860CFE0F4741B1D87A88A58FAE9A0B58EB@mailbe01.mc.vanderbilt.edu> Message-ID: <1696110849-1237557746-cardhu_decombobulator_blackberry.rim.net-2075945164-@bxe1028.bisx.prod.on.blackberry> Wow, what is the histogrl backlash? Will someone please summarize the issues people are having? Thanks! Andrea -----Original Message----- From: "Hofecker, Jennifer L" Date: Fri, 20 Mar 2009 08:23:02 To: ; Andrea Grantham Subject: RE: [Histonet] processing v-e-r-y tiny samples Hi Andi, Happy Friday! I know there's been a "Histogel backlash" of late, but I would still recommend trying it. I haven't had any problems. We use it for scant neuro specimens every day. We also have used H.G. for mouse sural nerve biopsies that needed to retain orientation (very tiny). If you do use Histogel, do not put it in a histoscreen or biopsy cassette and be sure to put it on a process that's long enough. People often use the cassette/process based on the size of the tissue, not of the Histogel block. I'd ask thermo for a sample of H.G. and try it. If you need any Histogel help, let me know. I'm a huge fan! Have a great day, Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Bryan Watson [mailto:Bryan.Watson@parkview.com] Sent: Thursday, March 19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! >>> Andrea Grantham 3/19/2009 11:51 AM >>> Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Mar 20 09:07:34 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Mar 20 09:08:07 2009 Subject: [Histonet] Histogel In-Reply-To: <1696110849-1237557746-cardhu_decombobulator_blackberry.rim.net-2075945164-@bxe1028.bisx.prod.on.blackberry> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> <49C27532.5674.0085.1@parkview.com> <5F3F860CFE0F4741B1D87A88A58FAE9A0B58EB@mailbe01.mc.vanderbilt.edu> <1696110849-1237557746-cardhu_decombobulator_blackberry.rim.net-2075945164-@bxe1028.bisx.prod.on.blackberry> Message-ID: <49C3A326.8090404@umdnj.edu> > histogrl backlash thats a whole 'nother set of issues altogether ;) happy friday! anh2006@med.cornell.edu wrote: > Wow, what is the histogrl backlash? Will someone please summarize the issues people are having? > > Thanks! > Andrea > > -----Original Message----- > From: "Hofecker, Jennifer L" > > Date: Fri, 20 Mar 2009 08:23:02 > To: ; Andrea Grantham > Subject: RE: [Histonet] processing v-e-r-y tiny samples > > > Hi Andi, Happy Friday! > I know there's been a "Histogel backlash" of late, but I would still > recommend trying it. I haven't had any problems. We use it for scant > neuro specimens every day. We also have used H.G. for mouse sural nerve > biopsies that needed to retain orientation (very tiny). If you do use > Histogel, do not put it in a histoscreen or biopsy cassette and be sure > to put it on a process that's long enough. People often use the > cassette/process based on the size of the tissue, not of the Histogel > block. I'd ask thermo for a sample of H.G. and try it. If you need any > Histogel help, let me know. I'm a huge fan! > Have a great day, > Jennifer > > Jennifer L. Hofecker HT(ASCP) > Vanderbilt University Medical Center > Division of Neuropathology > Nashville, TN > ph 615.343.0083 > fax 615.343.7089 > -----Original Message----- > From: Bryan Watson [mailto:Bryan.Watson@parkview.com] > Sent: Thursday, March 19, 2009 3:39 PM > To: histonet@lists.utsouthwestern.edu; Andrea Grantham > Subject: Re: [Histonet] processing v-e-r-y tiny samples > > I may never complain about tiny GI or bronch biopsies ever again! > > >>>> Andrea Grantham 3/19/2009 11:51 AM >>> >>>> > Good Morning! > In keeping with the weirdness of the projects I get in this lab today > my question is about processing mosquito GI tracts. > I have a processing schedule - that is not the problem. I'm wondering > if anybody out in histoland has a suggestion for what kind of > cassette to use. I was thinking of the histoscreen cassette because > these GI tracts are so thin (I think thinner than a hair)and I don't > want to wrap them or use sponges because I'm afraid that I'll loose > them or crush them. > Any ideas? > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kgreen <@t> hsh.org Fri Mar 20 09:17:55 2009 From: kgreen <@t> hsh.org (Green, Kathy) Date: Fri Mar 20 09:24:06 2009 Subject: [Histonet] "Employment Opportunity in Camp Hill, Pennsylvania" Message-ID: HISTOLOGY TECHNICIAN NEEDED! Holy Spirit Health System, a leading healthcare employer in South Central Pennsylvania, is currently seeking a full time Histology Technician. The ideal candidate will possess: * HT certification by Board of Registry of the American Society of Clinical Pathologists * Knowledge and understanding of histologic theory normally acquired through completion of one year at a certified school of histotechnology or through one year of on-the-job training * Strong interpersonal and communication skills * Full time, Day/Evening shift (12:00PM to 8:30PM) with some weekend rotation To apply, please visit our website at www.hsh.org or email aschied@hsh.org for additional information. Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From micropathlabs <@t> yahoo.com Fri Mar 20 09:28:19 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Mar 20 09:28:25 2009 Subject: [Histonet] Intellipath Instrument Message-ID: <10774.14470.qm@web57802.mail.re3.yahoo.com> A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the?Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know?nothing about?their instrumentation. Does anyone have any first hand?experience with?the Intellipath instrument, service from?Biocare, etc...? Any information would be helpful. Thank you in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From pkrichar <@t> gundluth.org Fri Mar 20 09:41:31 2009 From: pkrichar <@t> gundluth.org (pkrichar@gundluth.org) Date: Fri Mar 20 09:41:40 2009 Subject: [Histonet] PowerPath and Epic In-Reply-To: Message-ID: Hi, we are in the process of moving to PowerPath replacing our current pathology computer system. Our hospital is using Epic and I am wondering if anyone has PowerPath that receives orders from Epic and sends results back. I am really interested in how orders are placed in Epic. Thanks Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipients(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by return e-mail and destroy all copies of the original message. Thank you. From Stephen.Clark1 <@t> hcahealthcare.com Fri Mar 20 09:51:05 2009 From: Stephen.Clark1 <@t> hcahealthcare.com (Clark Stephen - Myrtle Beach) Date: Fri Mar 20 09:51:14 2009 Subject: [Histonet] Sure Path Message-ID: <213A638666ECDB4A8A81B5CF8646CC0B04408CBFC5@NADCWPMSGCMS05.hca.corpad.net> Hey everyone, We are most probably going to be using Sure path in the future, in conjunction with Meditech. Can anyone give me any insight as to the pro's and con's of Sure Path and is it "linkable" to Meditech? Steve Clark Grand Strand Regional Medical Center Myrtle Beach, SC From lblazek <@t> digestivespecialists.com Fri Mar 20 10:09:40 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Mar 20 10:05:26 2009 Subject: [Histonet] Intellipath Instrument In-Reply-To: <10774.14470.qm@web57802.mail.re3.yahoo.com> References: <10774.14470.qm@web57802.mail.re3.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390872FAF953@IBMB7Exchange.digestivespecialists.com> Sheila, I have had the Intellipath since December and I love it. Any service issues have been immediately answered. Support has been above and beyond expectation. If you have any specific questions I will be happy to answer them. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Friday, March 20, 2009 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the?Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know?nothing about?their instrumentation. Does anyone have any first hand?experience with?the Intellipath instrument, service from?Biocare, etc...? Any information would be helpful. Thank you in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Fri Mar 20 11:44:08 2009 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Mar 20 10:26:59 2009 Subject: [Histonet] Plastic storage containers for brain tissue? Message-ID: <49C3C7D8.9010304@mclean.harvard.edu> Hi Everyone: We are looking for rectangular plastic containers to store our brain tissue in. We use two sizes, a 60 oz container (for fixed half brains or their slices) and a 128 oz container (for whole fixed brains or their slices). Recently we have been unable to locate a vendor that makes these sizes anymore. Does anyone know of a vendor that still makes these containers? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center 617-855-3592 From margaret <@t> imebinc.com Fri Mar 20 10:37:09 2009 From: margaret <@t> imebinc.com (Margaret O'Donnell) Date: Fri Mar 20 10:37:23 2009 Subject: [Histonet] dako autostainers In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF81DF4FA@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF81DF4FA@VDXSERVER01.vdxpathology.local> Message-ID: <002f01c9a971$bb925da0$32b718e0$@com> Hi Jen, Hope it's OK for a vendor to jump in here. I'm with IMEB, INC. We offer the Dako stainer refurbished with updated software. Units are completely refurbished and include warranty, installation and training. Please contact me if we can be of service! Margaret O'Donnell Sales Rep IMEB, INC Tel: 800-543-8496 Fax: 760-761-0859 margaret@imebinc.com "The Pathology Specialists" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Thursday, March 19, 2009 8:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dako autostainers We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. Jen C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Mar 20 10:47:38 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 20 10:47:58 2009 Subject: [Histonet] Intellipath Instrument In-Reply-To: <10774.14470.qm@web57802.mail.re3.yahoo.com> Message-ID: We have also recently purchased this instrument. I highly recommend it. Sheila Haas Sent by: histonet-bounces@lists.utsouthwestern.edu 03/20/2009 09:28 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know nothing about their instrumentation. Does anyone have any first hand experience with the Intellipath instrument, service from Biocare, etc...? Any information would be helpful. Thank you in advance. Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Mar 20 12:30:33 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Mar 20 12:30:59 2009 Subject: [Histonet] Brain containers Message-ID: <8CB7798B52D1200-15F8-443@WEBMAIL-MY27.sysops.aol.com> im Wheelock asks about brain containers: We use the 5.7 liter polyethylene containers from Richard Allen (Cat # 5357). When fixing whole brains we suspend the brain in the formalin in a hair net. We keep the brain slices in these containers too. Michael Titford USA Pathology Mobile AL USA From cathy <@t> wasatchhisto.com Fri Mar 20 12:31:14 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Mar 20 12:31:36 2009 Subject: [Histonet] For Sale small lab equipment Message-ID: Dear Fellow Histonetters, I have 2 balances for sale 600 gm capacity, top load, each pass and are recertified every year balance weights for standarizing 3 Tissue Tek II staining trays and extra staining dishes and lots of Tissue Tek II staining racks 1 large glass dessicator 1 medium glass dessicator lots of consumables Cathy Mayton Wasatch Histo Consultants, Inc. From HornHV <@t> archildrens.org Fri Mar 20 12:35:51 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Mar 20 12:36:01 2009 Subject: [Histonet] list of special stains In-Reply-To: <424D930E0319E0469DD9C61502A50E300210217A@NIHCESMLBX4.nih.gov> References: <424D930E0319E0469DD9C61502A50E300210217A@NIHCESMLBX4.nih.gov> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830CD@EMAIL.archildrens.org> One of the histology text books??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, March 19, 2009 5:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] list of special stains Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From anh2006 <@t> med.cornell.edu Fri Mar 20 13:13:09 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Mar 20 13:13:20 2009 Subject: [Histonet] list of special stains In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830CD@EMAIL.archildrens.org> References: <424D930E0319E0469DD9C61502A50E300210217A@NIHCESMLBX4.nih.gov> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830CD@EMAIL.archildrens.org> Message-ID: DAKO has (or had as it's been some years since I got mine) a FREE Guide to Special Stains introductory manual, which was great for such info. >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, >Patricia (NIH/OD/ORS) [E] >Sent: Thursday, March 19, 2009 5:18 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] list of special stains > >Does anyone have or know where to obtain a list of special stains that >briefly describes which each one is for? > > > >Thanks, > > > >Patricia Zerfas > >National Institutes of Health > >Building 28A, Room 112 > >28 Library Drive > >Bethesda, MD 20892 > >ph: (301) 496-4464 > >fax: (301) 402-1068 -- From zodiac29 <@t> comcast.net Fri Mar 20 13:14:27 2009 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Fri Mar 20 13:14:30 2009 Subject: [Histonet] automatic coverslipper In-Reply-To: <1583912495.3414541237572794360.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <1119938813.3415091237572867249.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny From CIngles <@t> uwhealth.org Fri Mar 20 13:39:31 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Fri Mar 20 13:41:31 2009 Subject: [Histonet] processing v-e-r-y tiny samples References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> Message-ID: Careful - you may be close to the mark. Thermo laid off about 75 people here in Madison last week. Claire ________________________________ Shandon - or whatever they're called this week- From marktarango <@t> gmail.com Fri Mar 20 14:28:32 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 20 14:28:36 2009 Subject: [Histonet] list of special stains In-Reply-To: References: <424D930E0319E0469DD9C61502A50E300210217A@NIHCESMLBX4.nih.gov> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830CD@EMAIL.archildrens.org> Message-ID: <5b6eb13e0903201228k38565099lcf3b8571a1a18de9@mail.gmail.com> I got one from our Dako rep a few weeks ago. They do still have this and it's free. Mark On Fri, Mar 20, 2009 at 11:13 AM, Andrea Hooper wrote: > DAKO has (or had as it's been some years since I got mine) a FREE Guide to > Special Stains introductory manual, which was great for such info. > > > > -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, >> Patricia (NIH/OD/ORS) [E] >> Sent: Thursday, March 19, 2009 5:18 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] list of special stains >> >> Does anyone have or know where to obtain a list of special stains that >> briefly describes which each one is for? >> >> >> >> Thanks, >> >> >> >> Patricia Zerfas >> >> National Institutes of Health >> >> Building 28A, Room 112 >> >> 28 Library Drive >> >> Bethesda, MD 20892 >> >> ph: (301) 496-4464 >> >> fax: (301) 402-1068 >> > > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Mar 20 14:45:14 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 20 14:45:19 2009 Subject: [Histonet] automatic coverslipper In-Reply-To: <1119938813.3415091237572867249.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> Message-ID: <444031.43846.qm@web65709.mail.ac4.yahoo.com> For your work volumen as well as?for any other as well, get a Sakura film coverslipper. Ren? J. --- On Fri, 3/20/09, zodiac29@comcast.net wrote: From: zodiac29@comcast.net Subject: [Histonet] automatic coverslipper To: histonet@lists.utsouthwestern.edu Date: Friday, March 20, 2009, 2:14 PM Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Doug.Showers <@t> propath.com Fri Mar 20 15:00:59 2009 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Fri Mar 20 15:02:30 2009 Subject: [Histonet] automatic coverslipper In-Reply-To: <444031.43846.qm@web65709.mail.ac4.yahoo.com> References: <1119938813.3415091237572867249.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net> <444031.43846.qm@web65709.mail.ac4.yahoo.com> Message-ID: <82C7248978CB50469FD6BA68EBBEFE67EB478E@exchange.propathlab.com> The tape coverslipper from Sakura does work very well, but I would advise you to get buy in from your pathologists and anyone else that is going to be reading slides with the tape coverslips. Some people insist on glass coverslips. Tape coverslip advantages: 1) Speed (I have used one machine to coverslip 3-4,000 slides per day) 2) Quick drying (Important if you are a core lab and courier slides out somewhere) 3) Fast removal of coverslips (Several techniques are used, check the Histonet archives.) Glass Coverslip advantages 1) Saves tech time and reduces exposure to xylene. 2) Stays with traditional glass coverslip. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 214-237-1680 214-422-3083 Mobile 214-237-1706 FAX To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 20, 2009 2:45 PM To: histonet@lists.utsouthwestern.edu; zodiac29@comcast.net Subject: Re: [Histonet] automatic coverslipper For your work volumen as well as?for any other as well, get a Sakura film coverslipper. Ren? J. --- On Fri, 3/20/09, zodiac29@comcast.net wrote: From: zodiac29@comcast.net Subject: [Histonet] automatic coverslipper To: histonet@lists.utsouthwestern.edu Date: Friday, March 20, 2009, 2:14 PM Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jenee.S.Odani <@t> hawaii.gov Fri Mar 20 15:20:44 2009 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Fri Mar 20 15:20:12 2009 Subject: [Histonet] question regarding rules for disposal of slides & cassettes In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE67EB478E@exchange.propathlab.com> Message-ID: Not sure why we don't already have a policy for this, but my histotechnologist wanted me to find out how to properly dispose of stained/coverslipped slides and cassetes with FFPE tissue. Our lab only processes animal tissue (no TSE material). I tried searching the internet, but didn't find anything. Thanks in advance for any advice! -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 From godsgalnow <@t> aol.com Fri Mar 20 15:45:18 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Mar 20 15:45:42 2009 Subject: Fwd: [Histonet] Intellipath Instrument In-Reply-To: <8CB77B35EAA5F02-8A8-1514@WEBMAIL-MA07.sysops.aol.com> References: <10774.14470.qm@web57802.mail.re3.yahoo.com> <8CB77B35EAA5F02-8A8-1514@WEBMAIL-MA07.sysops.aol.com> Message-ID: <8CB77B3EA392AA3-8A8-154F@WEBMAIL-MA07.sysops.aol.com> Sorry.....forgot to reply to the list...see my response below -----Original Message----- From: godsgalnow@aol.com To: micropathlabs@yahoo.com Sent: Fri, 20 Mar 2009 4:41 pm Subject: Re: [Histonet] Intellipath Instrument This company has always provided the best service and we are getting 10 instruments, if that tells you anything Roxanne -----Original Message----- From: Sheila Haas To: histonet@lists.utsouthwestern.edu Sent: Fri, 20 Mar 2009 10:28 am Subject: [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the?Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know?nothing about?their instrumentation. Does anyone have any first hand?experience with?the Intellipath instrument, service from?Biocare, etc...? Any information would be helpful. Thank you in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet See Your Credit Report in Seconds! Easy to Read and Viewable Online. From JMacDonald <@t> mtsac.edu Fri Mar 20 15:45:14 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 20 15:47:38 2009 Subject: [Histonet] Current books for Histotechnology Message-ID: Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From rgrow <@t> bmnet.com Fri Mar 20 16:03:10 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Mar 20 16:03:14 2009 Subject: [Histonet] Re: dako autostainers In-Reply-To: Message-ID: Jennifer, I have an older model Dako Autostainer that still works well. Dako does still service it, too. It had a printer available, but we chose not to use it. Our volume is small, ~2400 slides yr. The size/type stainer you choose should be based on the number of immuno's that you do a year. NOT on what Dako wants to push on you, equipment or antibody systems. And I do mean PUSH. They tried for a year to Push their new FLEX system on us. It becomes a closed system and much more expensive. Do not allow them to do it to you. I was on the verge of going to a competitor. If you push back, they will back off. I still use Dako for all my IHC, but, again, I may start looking closer at the competition because the corporation is not giving the small lab the breaks that we need to keep up with the big hospitals. It's sad that the Dako leadership is not paying attention to their customers. Dako, Are you listening? I'm not sure how much this helps you, but I feel better by letting someone know that they are/were a good company, but is under bad leadership at the moment. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ------------------------------ Message: 5 Date: Thu, 19 Mar 2009 08:45:23 -0700 From: "Jennifer Campbell" Subject: [Histonet] dako autostainers To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF81DF4FA@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. From rosenfeldtek <@t> hotmail.com Fri Mar 20 16:45:56 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Mar 20 16:46:01 2009 Subject: [Histonet] processing v-e-r-y tiny samples In-Reply-To: <49C27532.5674.0085.1@parkview.com> References: <6.2.3.4.1.20090319084302.027167d0@algranth.inbox.email.arizona.edu> <49C27532.5674.0085.1@parkview.com> Message-ID: Well, you could always hand process them in test tubes. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 19 Mar 2009 16:39:14 -0400 > From: Bryan.Watson@parkview.com > To: histonet@lists.utsouthwestern.edu; algranth@u.arizona.edu > Subject: Re: [Histonet] processing v-e-r-y tiny samples > CC: > > I may never complain about tiny GI or bronch biopsies ever again! > > >>> Andrea Grantham 3/19/2009 11:51 AM >>> > Good Morning! > In keeping with the weirdness of the projects I get in this lab today > my question is about processing mosquito GI tracts. > I have a processing schedule - that is not the problem. I'm wondering > if anybody out in histoland has a suggestion for what kind of > cassette to use. I was thinking of the histoscreen cassette because > these GI tracts are so thin (I think thinner than a hair)and I don't > want to wrap them or use sponges because I'm afraid that I'll loose > them or crush them. > Any ideas? > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Internet Explorer 8 ? Now Available. Faster, safer, easier. http://clk.atdmt.com/MRT/go/141323790/direct/01/ From jshea121 <@t> roadrunner.com Sat Mar 21 06:13:09 2009 From: jshea121 <@t> roadrunner.com (Shea's) Date: Sat Mar 21 06:13:19 2009 Subject: [Histonet] Bridge for Meditech Client Server/Magic Pathology Modules Message-ID: Tiffany, I was searching Histonet to find the answer to a similar question, but you look like the only one out there with this interest. I know it was back in 07, but may I ask how did your interface finally work out? We also have Meditech Magic, and I am looking for a way to have our current reference labs PAP smear reports flow into our Pathology Module. It may not be as difficult since most of the data sections are standardized Bethesda format, but in the Cytology Diagnosis data section, we have set up questionnaires: SPECIMEN ADEQUACY: CATEGORIZATION: DESCRIPTIVE DIAGNOSIS: RECOMMENDATIONS: COMMENT: Any thoughts? Jan Shea From koellingr <@t> comcast.net Sat Mar 21 22:34:56 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Mar 21 22:35:00 2009 Subject: [Histonet] FW: CD86 - mouse In-Reply-To: <1051404821.3690471237691719429.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <1892152898.3692691237692896631.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Joost, I was curious myself what was out there and maybe someone has answered you but I haven't seen anything on the HistoNet.? Haven't done anything with murine CD86 (B7.2) for?3-4 years but this is what I can recall.? Of course you know the whole CD80/86/CD28/CTLA-4 costimulatory story is tough because of transient nature and upregulations during stimulation or disease. Using the GL-1 clone from BD (don't work for them but like their anti-mouse reagents), we got pretty good results on frozen mouse tissues with standard frozen fixation?and IHC. At least reasonable in that?staining corresponded to flow data from similar mouse tissues.? As far as FFPE mouse tissues, the best I could do was to get pretty good but not overly convincing data with retieval but with a tyramide amplification necessary.? In flow if you look at a one color diagram of staining (isotype and then GL-1) the peaks are not well separated even with something like LPS stimmed cells. In fact, sometimes the pos peak might move a log but the base of the peaks (isotype and GL-1) can overlap substantially.? On a flow diagram, shoulders and mean peak heights are easy to differentiate over neg or background but I could never really seperate out truely pos from truely negative from low positive in FFPE mouse like you can with flow.? So frozen was the way we went. And I know there is, at least in humans, soluble CD86 floating around due to shedding/alternative splicing. If that happens in mice (I'm clueless) that could contribute to background.? GL-1 clone in frozen mouse tissue was how I ended up going but haven't touched this for many years. Ray Raymond Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "J.P. Bruijntjes (Joost)" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 18, 2009 7:48:00 AM GMT -08:00 US/Canada Pacific Subject: [Histonet] FW: CD86 - mouse Hi histonetters ? Is really nobody working with cd86 on mouse tissues? ? Joost Bruijntjes TNO Quality of Life Zeist Holland ? ? TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer ? This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Sun Mar 22 07:16:38 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sun Mar 22 07:16:41 2009 Subject: [Histonet] Water...I need more input Message-ID: <38720.22150.qm@web65710.mail.ac4.yahoo.com> I still haven't gotten enough input on Distilled vs. DI water.? One person said there is a big difference and a few said there is not much difference at all.? We will primarily be using it for our water baths and making up reagents.? However, we may be getting IHC in the future.? It seems that for cost effectiveness distilled is the way to go, so currently I'm heading down that road.? Help!? Need to make a decision soon. ? On another note, ?what brands of ETOH is everyone using?? We use Surgipath.? Trying to find the cheapest.? Thanks!! From jfray80 <@t> hotmail.com Sun Mar 22 08:30:37 2009 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Sun Mar 22 08:30:41 2009 Subject: [Histonet] Sakura Microwave Processors Message-ID: Has anyone purchased and are using Sakura's Microwave processors. I would like some input please. I would prefer areas in Texas, Oklahoma, Arkansas. I am looking into the X50 small model. Please contact me at jfray80@hotmail.com. Thank You Histojoe _________________________________________________________________ Get quick access to your favorite MSN content with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A From rjbuesa <@t> yahoo.com Sun Mar 22 10:24:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 22 10:24:47 2009 Subject: [Histonet] Water...I need more input In-Reply-To: <38720.22150.qm@web65710.mail.ac4.yahoo.com> Message-ID: <473074.4785.qm@web65709.mail.ac4.yahoo.com> Let me try to help you Kristen: 1-Distilled water is obtained by condensing water vapors when any type of water is boiled and?by doing so the salts and solids components of the original water remain in the boiling pot, and the vapors are condensed as pure water that has been "distilled". 2- Deionized water eliminates all the ions from any type of water by filtering it through active membranes that catch the ions leaving the exiting water as free from ions or "deionized". With BOTH methods you will end with the same product, i.e. "pure" water = water without ions, salts or solids. Now IF you are goint to prepare your water yourself, deionizing is cheaper at the long run because it is less expensive and complicated to maintain, but if you are going to buy, buy the one that is cheapest for you, and either one will be good for any procedure in histology, including IHC, or your water bath. ? 3- As for which pure ethanol to buy, IF it is pure (or "200 proof" ethanol) you are talking about a pure chemical organic substance that it does not matter who manufactures or sells it has to have the same properties so, again, buy the cheapest one. For an article I just finished I needed prices and the cheapest "denatured ethanol", perfectly suitable for histology work (including reagents for HC), was sold by International Medical Equipments, Inc. or IMEB at a cost of $26.00 per gallon (check the web). I hope I have been able to have put to rest your queries. Ren? J. --- On Sun, 3/22/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Water...I need more input To: "histonet" Date: Sunday, March 22, 2009, 8:16 AM I still haven't gotten enough input on Distilled vs. DI water.? One person said there is a big difference and a few said there is not much difference at all.? We will primarily be using it for our water baths and making up reagents.? However, we may be getting IHC in the future.? It seems that for cost effectiveness distilled is the way to go, so currently I'm heading down that road.? Help!? Need to make a decision soon. ? On another note, ?what brands of ETOH is everyone using?? We use Surgipath.? Trying to find the cheapest.? Thanks!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jodiputnam <@t> aol.com Sun Mar 22 13:03:40 2009 From: jodiputnam <@t> aol.com (jodiputnam@aol.com) Date: Sun Mar 22 13:03:55 2009 Subject: [Histonet] Immunoflourescence controls for derm specimens Message-ID: <8CB792FAA760E04-A1C-47F7@webmail-da09.sysops.aol.com> I need some info on obtaining controls for my immunoflourescence stains. I work in a derm lab, just opened. I just did?IF on a skin punch IgA, IgG, IgM and C3. In the past I used positive patient skins for controls for everything except when I needed to run fibrinogen, I used kidney tissue for control.? I was instructed to use tonsil for the IgA, IgM and IgG this time. I have never used tonsil before. Has anyone else? The doctor didn't think the tonsil reacted as positively as it should. What are you all using for controls for the panel I just listed? Obviously obtaining control tissue is difficult, especially in a start up lab. Any feedback would be appreciated. Thanks, Jodi From histopathy <@t> hotmail.com Sun Mar 22 14:51:08 2009 From: histopathy <@t> hotmail.com (Pamela Gholston) Date: Sun Mar 22 14:51:10 2009 Subject: [Histonet] Please remove me!!! Message-ID: Please remove me from the mailing list!! Pam Gholston From AnthonyH <@t> chw.edu.au Sun Mar 22 16:56:55 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Mar 22 16:57:17 2009 Subject: [Histonet] Water...I need more input In-Reply-To: <38720.22150.qm@web65710.mail.ac4.yahoo.com> Message-ID: This might help: Water Types Tap water will vary depending on location and season of the year. It is usually a very dilute solution of the carbonates and chlorides of calcium, sodium, and copper. Distilled water is produced by heating water to evaporation and then condensing it on a cold surface. During this process most impurities are either evaporated off ahead of the water (in the case of most organics), or left behind (in the case of minerals). The water is also effectively deionized because the salts are left behind. It is fairly pure water. To get very pure water it needs to be re-distilled several times. Deionized water is produced by passing water through a salt bed or ionized resin bed that captures the mineral ions (ie, a "water softener"). The water is not necessarily pure, however, especially in regards to organic chemicals. Reverse osmosis is also used now days to deionize water. To produce pure water, boil 600 ml of deionized water and add 6 mg of potassium permanganate to it and distil the water. Discard the first 100 ml of distillate. The next 300 ml will be very pure water. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Sunday, 22 March 2009 11:17 PM To: histonet Subject: [Histonet] Water...I need more input I still haven't gotten enough input on Distilled vs. DI water.? One person said there is a big difference and a few said there is not much difference at all.? We will primarily be using it for our water baths and making up reagents.? However, we may be getting IHC in the future.? It seems that for cost effectiveness distilled is the way to go, so currently I'm heading down that road.? Help!? Need to make a decision soon. ? On another note, ?what brands of ETOH is everyone using?? We use Surgipath.? Trying to find the cheapest.? Thanks!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JMacDonald <@t> mtsac.edu Sun Mar 22 19:51:10 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Mar 22 19:51:41 2009 Subject: [Histonet] Please remove me!!! In-Reply-To: <000501c9ab27$8b2bd670$a1838350$@com> Message-ID: Follow the link at the bottom for the histonet. It will give you the instructions for taking yourself off of the list!! "Pamela Gholston" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/22/2009 12:54 PM To cc Subject [Histonet] Please remove me!!! Please remove me from the mailing list!! Pam Gholston _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Sun Mar 22 22:00:37 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Sun Mar 22 22:05:07 2009 Subject: [Histonet] Ephrins/neuropilins Message-ID: <605260030-1237777502-cardhu_decombobulator_blackberry.rim.net-602002968-@bxe1029.bisx.prod.on.blackberry> Does anyone know good antibodies for staing murine EphrinB2 and Eph4? How about neuropilins 1 and 2? Thanks. From ooi.ting.huay <@t> nhc.com.sg Mon Mar 23 00:44:16 2009 From: ooi.ting.huay <@t> nhc.com.sg (ooi.ting.huay@nhc.com.sg) Date: Mon Mar 23 00:44:35 2009 Subject: [Histonet] (no subject) Message-ID: Hi, Is anyone know what is the different between modified movat pentachrome staining and the elastica van gieson staining? I am looking at this two staining. But could not figure out what is the different between it. Also, do anyone know who is selling the movat pentachrome staining? Please advice, thank you very much! Regards, Singapore Ooi _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer From SwainFrancesL <@t> uams.edu Mon Mar 23 07:17:30 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Mon Mar 23 07:18:15 2009 Subject: [Histonet] question regarding rules for disposal of slides & cassettes In-Reply-To: References: <82C7248978CB50469FD6BA68EBBEFE67EB478E@exchange.propathlab.com> Message-ID: Hi Jenee: You need to check the histonet archieves. For the last two weeks there has been an on going conversation in regard to disposal of microscopic slides. Since I am in a COR lab most of my slides are sent to the PI's that order the slides therefore I do not have to deal with this as of yet. But some folks put there in sharps containers or boxes which are labeled sharps some have other methods. Check it out Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenee.S.Odani@hawaii.gov Sent: Friday, March 20, 2009 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question regarding rules for disposal of slides & cassettes Not sure why we don't already have a policy for this, but my histotechnologist wanted me to find out how to properly dispose of stained/coverslipped slides and cassetes with FFPE tissue. Our lab only processes animal tissue (no TSE material). I tried searching the internet, but didn't find anything. Thanks in advance for any advice! -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sbreeden <@t> nmda.nmsu.edu Mon Mar 23 08:34:20 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Mar 23 08:34:25 2009 Subject: [Histonet] OT: Books Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6777@nmdamailsvr.nmda.ad.nmsu.edu> Gosh - my retirement fund just appeared... a Sheehan for $2500? Wow! I have one here (2nd edition) but not mine to sell - besides, I use it. I do have a 3rd edition Lillie "Histopathologic Technic and Practical Histochemistry" (1965), pre-autographed by me when I bought it that I'd sell for the right bid... think I could get enough for that condo in Costa Rica? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lblazek <@t> digestivespecialists.com Mon Mar 23 09:33:26 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Mar 23 09:29:05 2009 Subject: [Histonet] RE: OT: Books In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6777@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6777@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390872FAF961@IBMB7Exchange.digestivespecialists.com> Make sure you get enough to have visitors! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, March 23, 2009 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Books Gosh - my retirement fund just appeared... a Sheehan for $2500? Wow! I have one here (2nd edition) but not mine to sell - besides, I use it. I do have a 3rd edition Lillie "Histopathologic Technic and Practical Histochemistry" (1965), pre-autographed by me when I bought it that I'd sell for the right bid... think I could get enough for that condo in Costa Rica? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Mon Mar 23 10:13:11 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Mar 23 10:13:17 2009 Subject: [Histonet] Quality Improvement Message-ID: <57BE698966D5C54EAE8612E8941D7683053EBBC6@EXCHANGE3.huntingtonhospital.com> I'm curious as to what others do, if anything, to document corrective actions or improvements for H&E staining and/or tissue processing. For example, I made changes to my biopsy processing program and to my breast processing program which helped improve the cutting quality of both types of tissue. I also had to make various changes to my H&E staining program when we received hematoxylin and eosin from a new vendor. Would you document these changes somewhere? Would you have one general binder, for example, that would just be an ongoing log of changes/improvements, or would you document the changes and keep with the equipment? I'm just thinking this might be something that a CAP inspector would ask for, although I don't see any specific CAP question on the checklist. Laurie Colbert From b-frederick <@t> northwestern.edu Mon Mar 23 10:16:00 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Mar 23 10:16:16 2009 Subject: [Histonet] RE: OT: Books In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390872FAF961@IBMB7Exchange.digestivespecialists.com> Message-ID: So what does an Anne Preece go for? Can I retire too? I've got one of those Sheehan's too. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, March 23, 2009 9:33 AM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: OT: Books Make sure you get enough to have visitors! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, March 23, 2009 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Books Gosh - my retirement fund just appeared... a Sheehan for $2500? Wow! I have one here (2nd edition) but not mine to sell - besides, I use it. I do have a 3rd edition Lillie "Histopathologic Technic and Practical Histochemistry" (1965), pre-autographed by me when I bought it that I'd sell for the right bid... think I could get enough for that condo in Costa Rica? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Mon Mar 23 10:55:27 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon Mar 23 10:58:08 2009 Subject: [Histonet] Current books for Histotechnology References: Message-ID: I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Mon Mar 23 11:08:46 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon Mar 23 11:09:19 2009 Subject: [Histonet] More about distilled water Message-ID: <8CB79E8C7DBB797-B88-332@WEBMAIL-DY11.sysops.aol.com> I would like to add to the cooments of others concerning distilled water and deionised water: 1) I think most histology labs use deionised water as it is cheaper and easier? to obtain. 2) However, even deionised water will turn red with Schiff reagent. 3) For silver stain solutions, our lab uses "triple deionised" where the water passes through three tanks of resin, giving greater purity. 4) If your lab is required to be CAP complient, you will need monthly to check the resistivity and get a sample cultured.(It makes good sense even if you do not have to be CAP complient). 5) Deionised water systems can start growing Gram negative organisms in the tubing so you may want to put a filter over the actual spigot, and change that periodically. Mike Titford USA Pathology Mobile AL USA From igor.deyneko <@t> gmail.com Mon Mar 23 11:39:37 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Mar 23 11:39:41 2009 Subject: [Histonet] Murine Desmoplasia Markres Message-ID: <35e16a770903230939h42874755oc8a30edf3c59dbb6@mail.gmail.com> Dear Histonetters! I was wondering if anyone is working with Desmoplasia markers? I am working with xenograft models (human tumors implanted in mice). So the result is human tumor cells and Murine stroma. I am trying to test various desmoplasia markers found in literature, but almost all of them are for human stroma. So far I got Collagen and Fibronectin working beautifully, but I have problems with the following proteins: alpha Smooth Muscle Actin *Desmin* *GFAP (glial fibrillary actin protein)* *CD 31 (so I'm switching to CD34)* *SPARC* I have a lot of cross-reactivity issues. If anyone knows of antibodies , which are capable of identifying the Murine structures, while keeping the human tissues clean, please let me know. Any information would be highly appreciated! Thank you. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 From matthewtclose <@t> gmail.com Mon Mar 23 11:54:11 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Mon Mar 23 11:54:16 2009 Subject: [Histonet] re: water Message-ID: <6abc767b0903230954l670a4684s8daa84e62ceb7234@mail.gmail.com> I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the tap source, I would say you are probably okay going with the distilled water. My word of caution is to check the level of air in your housing tank and never let it drop below half. Typically, the more air in the tank, the more CO2 going into the water which will gradually lower the pH down to 5.1-5.5 territory. Also, if you have the option to chose between using a glass carboy and a plastic one to store water, always go with the glass. While all of this has never been a real problem for me in terms of analyzable results, I often wonder if it has anything to do with why, sometimes, identical staining procedures on identially fixed tissues will stain notably different. Matt Close Department of Biological Sciences Lehigh University From matthewtclose <@t> gmail.com Mon Mar 23 11:55:03 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Mon Mar 23 11:55:06 2009 Subject: [Histonet] re:water question Message-ID: <6abc767b0903230955v4ea04756mf60cd5c13ca91020@mail.gmail.com> I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the tap source, I would say you are probably okay going with the distilled water. My word of caution is to check the level of air in your housing tank and never let it drop below half. Typically, the more air in the tank, the more CO2 going into the water which will gradually lower the pH down to 5.1-5.5 territory. Also, if you have the option to chose between using a glass carboy and a plastic one to store water, always go with the glass. While all of this has never been a real problem for me in terms of analyzable results, I often wonder if it has anything to do with why, sometimes, identical staining procedures on identially fixed tissues will stain notably different. Matt Close Department of Biological Sciences Lehigh University From matthewtclose <@t> gmail.com Mon Mar 23 11:55:48 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Mon Mar 23 11:55:56 2009 Subject: [Histonet] re:water question Message-ID: <6abc767b0903230955g275ef2c7r78ba28f844e041cc@mail.gmail.com> I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the tap source, I would say you are probably okay going with the distilled water. My word of caution is to check the level of air in your housing tank and never let it drop below half. Typically, the more air in the tank, the more CO2 going into the water which will gradually lower the pH down to 5.1-5.5 territory. Also, if you have the option to chose between using a glass carboy and a plastic one to store water, always go with the glass. While all of this has never been a real problem for me in terms of analyzable results, I often wonder if it has anything to do with why, sometimes, identical staining procedures on identially fixed tissues will stain notably different. Matt Close Department of Biological Sciences Lehigh University From azdudley <@t> hotmail.com Mon Mar 23 11:56:00 2009 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Mar 23 11:56:04 2009 Subject: [Histonet] wondering if anyone else has had this problem? wh... Message-ID: wondering if anyone else has had this problem? when embedding small core bxs they are like they are charged and go off of the forceps to the edge of the embedding mold. its like they are running away from the forceps. what would be causing this? thanks so much. anita dudley providence hosp mobile, alabama _________________________________________________________________ Get quick access to your favorite MSN content with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A From elockman <@t> apsemail.com Mon Mar 23 12:37:35 2009 From: elockman <@t> apsemail.com (Emily Lockman) Date: Mon Mar 23 12:37:41 2009 Subject: [Histonet] deplastisizing Spurr Message-ID: <037BDA8D37760D49A2A23D1C877EA8C9AA727A@apsdc01.aps.dom> I'm wondering if anyone has any helpful tips for deplastisizing Spurr. We currently use saturated Sodium Hydroxide in 100% alcohol. We tried a 5% and a 1% solution as well, but still not perfect. The main issue we are having is the tissue is lifting off of the slide during staining. For microtomy, the charged slides are laid out on a warming plate, section is placed on the slide and adjusted with a drop of 80% alcohol, then pressed with filter paper to remove excess liquid. The slides are dried in a press for 48 hours after microtomy. Any information would be greatly appreciated. Thanks, Emily From TJJ <@t> stowers.org Mon Mar 23 12:54:37 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Mar 23 12:55:20 2009 Subject: [Histonet] Re: wondering if anyone else has had this problem? Message-ID: Anita, I have seen this when using plastic embedding molds. The molds themselves hold an electric charge. It's annoying when your samples all migrate to the edges. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From ASelf <@t> georgetownhospitalsystem.org Mon Mar 23 13:10:35 2009 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Mon Mar 23 13:10:43 2009 Subject: [Histonet] JCAHO - Universal Protocol Message-ID: Dear Histonetters, Is there anyone out there that follows the JCAHO - Universal Protocol/Time Out procedure when the pathologist perform his/her own FNA's? We have a pathologist that will perform FNA's when asked and I was told that when he performs these procedures that we need to be following this universal protocol/time out procedure that is followed in the OR for surgeries. I have all the info. from the OR but to me the procedure that they follow is just too in-depth for the palpable masses that are aspirated for cytology. So I am turning to you all for help. I was just wondering what other facilities were doing about this. Any help would be appreciated. Thanks in advance. Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From trathborne <@t> somerset-healthcare.com Mon Mar 23 13:29:19 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Mar 23 13:29:25 2009 Subject: [Histonet] Morgue lift Message-ID: I'm hoping someone can help me. We are looking for a copy of the Operations Manual for a lift made by Lipshaw, Model 15BS. We use this for moving bodies in our morgue, and can not find information about servicing it. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From anieto <@t> anapath.es Wed Mar 18 12:14:37 2009 From: anieto <@t> anapath.es (anieto) Date: Mon Mar 23 13:29:44 2009 Subject: [Histonet] Calcium scaffold and decalcification Message-ID: <1F62C7826C05443B853254339995D84D@ANAISABELNIETO1> I have running a experiment in rabbit that included the bone reconstruction using mesenchymal stem cell seeded previously in calcium phosphate and calcium silicate scaffold. I need to know if, after some time, the scaffold remains in the tissue or they are reabsorbed and the bone is reconstruted. The technique is parafin embeded tissues. My question is: what kind of decalcification have I to used to decalcificate the bone but that permit me to see the remained calcium phosphate and calcium silicate scaffold?. Could the acid or EDTA eliminate completly the remained scaffold? Any opinion on your experience is appreciated. Sincerely Ana I. Nieto Anapath Pathology Diagnosis anieto@anapath.es www.anapath.es From lblazek <@t> digestivespecialists.com Mon Mar 23 13:51:16 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Mar 23 13:46:52 2009 Subject: [Histonet] RE: Quality Improvement In-Reply-To: <57BE698966D5C54EAE8612E8941D7683053EBBC6@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683053EBBC6@EXCHANGE3.huntingtonhospital.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390872FAF968@IBMB7Exchange.digestivespecialists.com> Laurie, I have a binder that is for Continuous Quality Improvement. If something comes up that is not part of my regular schedule of Quality Control or Quality Improvement it goes in that binder. I have a form that I use to state the reason for the Quality Improvement (what initiated the change), what the standard is and what the change is. It is then signed off by the pathologist and all staff initials the change. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, March 23, 2009 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quality Improvement I'm curious as to what others do, if anything, to document corrective actions or improvements for H&E staining and/or tissue processing. For example, I made changes to my biopsy processing program and to my breast processing program which helped improve the cutting quality of both types of tissue. I also had to make various changes to my H&E staining program when we received hematoxylin and eosin from a new vendor. Would you document these changes somewhere? Would you have one general binder, for example, that would just be an ongoing log of changes/improvements, or would you document the changes and keep with the equipment? I'm just thinking this might be something that a CAP inspector would ask for, although I don't see any specific CAP question on the checklist. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Mon Mar 23 14:15:57 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Mar 23 14:16:02 2009 Subject: [Histonet] microtome Message-ID: What is the best microtome on the market these days? We are a research lab sectioning mainly mouse tissue, and mainly decalcified bone. We have beginners to experts in the lab all who will need to use it. It's been a while since I have been in the market for a microtome, so although I have my own opinion already I wanted to know what the current thoughts are - in case I am missing something. Thanks, ANDREA -- From mucram11 <@t> comcast.net Mon Mar 23 14:38:33 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Mar 23 14:38:36 2009 Subject: [Histonet] microtome In-Reply-To: <905103027.121021237836964219.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <704141617.121601237837113892.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We are also a research lab and do both decal and non-decal bone from mouse to horse.? We have three Leicas, 2 RM 2255 and one older RM2155.? They are all motorized and we love them with tungsten carbide or disposable knives.? We have them for 3 and 4 years with never a problem. Pam Marcum UPENN Vet School New Bolton Center ----- Original Message ----- From: "Andrea Hooper" To: "Histonet" Sent: Monday, March 23, 2009 3:15:57 PM GMT -05:00 US/Canada Eastern Subject: [Histonet] microtome What is the best microtome on the market these days? We are a research lab sectioning mainly mouse tissue, and mainly decalcified bone. We have beginners to experts in the lab all who will need to use it. It's been a while since I have been in the market for a microtome, so although I have my own opinion already I wanted to know what the current thoughts are - in case I am missing something. Thanks, ANDREA -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jenee.S.Odani <@t> hawaii.gov Mon Mar 23 15:50:05 2009 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Mon Mar 23 15:49:32 2009 Subject: [Histonet] question regarding rules for disposal of slides & cassettes Message-ID: Thanks for the tip! I was sure thi actually didn't know about the archives un did a search for it just now. Thanks so much! Jenee S. Odani, D.V.M., Dip Veterinary Medical Officer Hawaii State Veterinary 99-941 Halawa Valley Street, Aiea, HI, 96701 Phon < inFrancesL@uams.edu> To "Jenee.S.Odani "histonet@lists.ut bcc Subject RE: [Histonet] question regarding rules for disposal of slides & Hi Jenee: You need to check the histo two weeks there has been an on going conv disposal of microscopic slides. Since I am in a slides are sent to the PI's that order the slides there have to deal with this as of yet. But some folks put th sharps containers or boxes which are labeled sharps some have other methods. Check it out Frances L. Swain HT(ASCP) A. A. S. Speci Department of Orthopaedic Surgery Barton Research Building 2R28< Little Rock AR 72205 (501) (501) 686-8987 FAX swainfrancesl@uams.edu e -----Original Message----- From: histonet-bounces@list [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Je nee.S.Odani@hawaii.gov Sent: Friday, March 20, 2009 3:21 PM To:& Subject: [Histonet] question cassettes Not sure why we histotechnologist wan stained/coverslipped only processes animal internet, but didn Thanks in advance for any advice! -J Jenee S. Veterinary Medical Officer Hawaii&nbs 99-941 Halawa Valley Street, Aiea Phone: (808) 483-7131/Fax: (808) 483-7110 ___ _______________________ 5F_ Histonet Histonet@lists.utsouthwestern.edu [2]http://lists.utsouthwestern.e Confidentiality Notice: This e-mail me attachments, is for the sole use of the intended recip may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. I reply e- References 1. 3Dmailto:histonet-bounces@lists.utsouthwester 2. 3Dhttp://lists=/ From AnthonyH <@t> chw.edu.au Mon Mar 23 16:29:25 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Mar 23 16:29:32 2009 Subject: [Histonet] Quality Improvement In-Reply-To: <57BE698966D5C54EAE8612E8941D7683053EBBC6@EXCHANGE3.huntingtonhospital.com> Message-ID: I am a firm believer in lab notebooks. In it we record all routine maintenance, quality control, solution preparation (especially dye lot numbers, source etc) as well as variations to technique caused by vagaries of dye reagents. Any developmental work is also recorded, trial of new dyes, techniques. Source of good control material. I find them of great use especially when I have to repeat a technique that was ex-manual, ie the odd stain that is requested on a particular case, done so rarely that it would not appear in a routine stain manual. It saves me having to re-invent the wheel. The best method of documenting quality control. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, 24 March 2009 2:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quality Improvement I'm curious as to what others do, if anything, to document corrective actions or improvements for H&E staining and/or tissue processing. For example, I made changes to my biopsy processing program and to my breast processing program which helped improve the cutting quality of both types of tissue. I also had to make various changes to my H&E staining program when we received hematoxylin and eosin from a new vendor. Would you document these changes somewhere? Would you have one general binder, for example, that would just be an ongoing log of changes/improvements, or would you document the changes and keep with the equipment? I'm just thinking this might be something that a CAP inspector would ask for, although I don't see any specific CAP question on the checklist. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From shive003 <@t> umn.edu Mon Mar 23 17:00:00 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Mar 23 17:01:46 2009 Subject: [Histonet] Quality Improvement References: <57BE698966D5C54EAE8612E8941D7683053EBBC6@EXCHANGE3.huntingtonhospital.com> Message-ID: <7A4E549977014A7492E028366463DA51@auxs.umn.edu> Laurie, et al. - This is what we do in my lab... We use a computer quality control program called QPulse. Everytime an SOP is changed (even slightly, such as vendor or incubation time change), the new revision is made through the QPulse Document Draft program, and in the process of making this revision permanent, one must state what changes have been made in a Comments box. The SOP creator or another authorized person within your lab must OK the new revision (online through the QP program), and then a notification is sent out via email to all pertinent lab staff alerting them to the new revision. They must then Acknowledge receipt of this email (online again), so that you are aware of who has actually read the new revision. All of these actions are recorded in QPulse forever. The old revision becomes Obsolete and is permanently moved within the QPulse computer program to an Obsolete folder (so if you needed to look it up in years to come, it's still there). Paper copies (if you use them) are collected, stamped Obsolete, and put into an Obsolete binder stored in another room. The QPulse program also has a feature that will alert the lab supervisor via email when an SOP is up for reviewal, if you have a system of annual reviewals in your institution. This is a very handy reminder tool. Jan Shivers Section Head IHC/Histo/EM UMN Vet Diag Lab St. Paul, MN ----- Original Message ----- From: "Laurie Colbert" To: Sent: Monday, March 23, 2009 10:13 AM Subject: [Histonet] Quality Improvement I'm curious as to what others do, if anything, to document corrective actions or improvements for H&E staining and/or tissue processing. For example, I made changes to my biopsy processing program and to my breast processing program which helped improve the cutting quality of both types of tissue. I also had to make various changes to my H&E staining program when we received hematoxylin and eosin from a new vendor. Would you document these changes somewhere? Would you have one general binder, for example, that would just be an ongoing log of changes/improvements, or would you document the changes and keep with the equipment? I'm just thinking this might be something that a CAP inspector would ask for, although I don't see any specific CAP question on the checklist. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Mon Mar 23 17:40:11 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Mar 23 17:40:42 2009 Subject: [Histonet] cassette storage/cardboard slide holder for sale Message-ID: <072D1BA72D18405A94BE524CAD919E53@shop1e2e996aa5> For Sale 2 set of paraffin block drawers $25 20 or better cardboard slide holders (holds 20 slides) $1 each Cathy Mayton Wasatch Histo Consultants, Inc. From chesarato <@t> hotmail.com Mon Mar 23 23:00:39 2009 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Mon Mar 23 23:00:43 2009 Subject: [Histonet] C4d Antibody Message-ID: Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. C?sar Romero Buenos Aires Argentina _________________________________________________________________ ?Quer?s saber c?mo va a estar el clima ma?ana? Ingres? ahora a MSN http://tiempo.ar.msn.com/ From kappeler <@t> patho.unibe.ch Tue Mar 24 01:15:04 2009 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Mar 24 01:15:17 2009 Subject: AW: [Histonet] C4d Antibody In-Reply-To: References: Message-ID: <000901c9ac47$df0bfc90$17955c82@pi23> Hi C?sar we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for cryosections of kidney biopsies (indirect immunofluorescence), it won't work on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d (BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH buffer (Tris-EDTA, pH 9.0; Tris-Urea, pH 9.5). Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. M?rz 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. C?sar Romero Buenos Aires Argentina _________________________________________________________________ ?Quer?s saber c?mo va a estar el clima ma?ana? Ingres? ahora a MSN http://tiempo.ar.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jorge.tornero <@t> gmail.com Tue Mar 24 02:20:57 2009 From: jorge.tornero <@t> gmail.com (Jorge Tornero) Date: Tue Mar 24 02:21:08 2009 Subject: [Histonet] Substitutes for Technovit 7100 Message-ID: <8c964a790903240020m1176b686h5f32bcf92d0fa812@mail.gmail.com> Hi all, I am lookin for a substitute for Technovit 7100 in our research (currently on fish female gonad). Any help or suggestions about this point would be very very appreciated. Best regards Jorge Tornero IEO - C?diz- Spain From akbitting <@t> geisinger.edu Tue Mar 24 08:24:08 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Mar 24 08:24:47 2009 Subject: [Histonet] C4d Antibody In-Reply-To: References: Message-ID: <49C8A6B7.2B7F.00C9.0@geisinger.edu> We get our from Alpco. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Cesar Francisco Romero 3/24/2009 12:00 AM >>> Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. C?sar Romero Buenos Aires Argentina _________________________________________________________________ ?Quer?s saber c?mo va a estar el clima ma?ana? Ingres? ahora a MSN http://tiempo.ar.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From relia1 <@t> earthlink.net Tue Mar 24 08:40:23 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Mar 24 08:40:26 2009 Subject: [Histonet] RELIA JOB ALERT Brand New Start-up Specialty Lab in Poughkeepsie NY!! Message-ID: Hi Histonetters! I hope everyone is having a great day. I have a new opportunity I want to tell you about! I am working with a brand new specialty lab opening in the Poughkeepsie area. They are in need of 2 histotechs 1-junior and 1 senior tech. for full time permanent day shift positions. The shift is 8-5 M-F and T-S you would be alternating the Saturdays. They are looking for people who are experienced in routine histology including cutting, embedding special stains -immunos are a plus. A New York State license and ASCP certification are not required for these jobs. My client offers a competitive salary, benefits and the opportunity to participate in the start up of a brand new histology lab. If you or someone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From arvidsonkristen <@t> yahoo.com Tue Mar 24 09:44:24 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Mar 24 09:44:31 2009 Subject: [Histonet] hematoxylin Question Message-ID: <939251.95015.qm@web65713.mail.ac4.yahoo.com> Hello All, How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is poured into a staining container?? The stain gets used so infrequently I would like to keep it as long as I can.? We currently dump it weekly regardless of use.? Thank you. ? -Kristen From gu.lang <@t> gmx.at Tue Mar 24 10:25:34 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 24 10:25:43 2009 Subject: AW: [Histonet] C4d Antibody In-Reply-To: References: Message-ID: Our's is from Zytomed, a polyclonal rabbit (a12-5000). We work with the benchmark XT, 1:25 with amplifier. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. M?rz 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. C?sar Romero Buenos Aires Argentina _________________________________________________________________ ?Quer?s saber c?mo va a estar el clima ma?ana? Ingres? ahora a MSN http://tiempo.ar.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Tue Mar 24 10:35:39 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Mar 24 10:35:42 2009 Subject: [Histonet] Thanks for all the formalin waste responses Message-ID: <952893.36651.qm@web82005.mail.mud.yahoo.com> ? ? Hi All I just wanted to say thanks to everyone who has responded to my question regarding formalin waste going down the drain. I appreciate it! Have a great day! Jessica Piche-Grocki, HT(ASCP) From SwainFrancesL <@t> uams.edu Tue Mar 24 10:54:36 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Tue Mar 24 10:55:17 2009 Subject: [Histonet] hematoxylin Question In-Reply-To: <939251.95015.qm@web65713.mail.ac4.yahoo.com> References: <939251.95015.qm@web65713.mail.ac4.yahoo.com> Message-ID: What I do Kristen if I use it infrequently is I get a large brown bottle, label it with working hematoxylin. After I use the hematoxylin for whatever I am using it for I pour it in the brown bottle, cap it tightly and store it on the shelf or in a cabinet. Whenever I need it again I get it out, set up a filtering stand with a funnel, filtering paper etc and filter it back into the dish to be used again. You can test your hematoxylin to see if it is still good by dropping a drop in either distilled water or 37% formaldehyde. It is turns blue purple it is still good if it turns a mauve color toss it. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, March 24, 2009 9:44 AM To: histonet Subject: [Histonet] hematoxylin Question Hello All, How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is poured into a staining container?? The stain gets used so infrequently I would like to keep it as long as I can.? We currently dump it weekly regardless of use.? Thank you. ? -Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From vjp2105 <@t> columbia.edu Tue Mar 24 11:17:06 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Mar 24 11:17:12 2009 Subject: [Histonet] IF Message-ID: Hi everyone, When carrying out IF on frozen sections how long after the cutting the sections should you leave the slides to dry and where before starting the staining procedure? Thanks a mill, V From leiker <@t> buffalo.edu Tue Mar 24 12:00:06 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 24 12:00:13 2009 Subject: [Histonet] IF In-Reply-To: References: Message-ID: We don't let our unfixed sections air-dry (room temp) long before fixing them - a few minutes? For some people here I let them sit a few minutes then just store them in the -80 for up to a weeks weeks before they fix them and commence with their staining procedure. If the animals were perfusion-fixed maybe they can air-dry longer. I've heard everything from 5 min to overnight. At room temperature. Just my input. --On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan" wrote: > Hi everyone, > > When carrying out IF on frozen sections how long after the cutting the > sections should you leave the slides to dry and where before starting the > staining procedure? > > Thanks a mill, > > V > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From leiker <@t> buffalo.edu Tue Mar 24 12:08:23 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 24 12:08:28 2009 Subject: [Histonet] IF In-Reply-To: References: Message-ID: <84540F0A62C927C6975420C7@bchwxp2702.ad.med.buffalo.edu> sorry, that should read "for up to a few weeks." --On Tuesday, March 24, 2009 1:00 PM -0400 Merced Leiker wrote: > We don't let our unfixed sections air-dry (room temp) long before fixing > them - a few minutes? For some people here I let them sit a few minutes > then just store them in the -80 for up to a weeks weeks before they fix > them and commence with their staining procedure. If the animals were > perfusion-fixed maybe they can air-dry longer. I've heard everything from > 5 min to overnight. At room temperature. > > Just my input. > > --On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan" > wrote: > >> Hi everyone, >> >> When carrying out IF on frozen sections how long after the cutting the >> sections should you leave the slides to dry and where before starting the >> staining procedure? >> >> Thanks a mill, >> >> V >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From TJJ <@t> stowers.org Tue Mar 24 12:25:57 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Mar 24 12:26:33 2009 Subject: [Histonet] Re: IF Message-ID: Vanessa, The answer of how long is suitable, the answer is "it depends". Routinely, many will air dry over night, even over a fan, in order to achieve the best adherence to the glass slide. We have found that we lose immunoreactivity with some antigens if we let it go that long. Our routine has now changed to 2 hour drying time. We tried 1 hour but the researchers had trouble with the tissues (especially neural tissue) trying to lift off during the staining procedure. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From tim.morken <@t> thermofisher.com Tue Mar 24 12:38:37 2009 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue Mar 24 12:39:05 2009 Subject: [Histonet] Reference or service lab that does PDGFR-a staining? Message-ID: Hi, does anyone know of a reference or service lab that does immuno staining for PDGFR-a,b (platelet derived growth factor receptor alpha and/or beta. Please note my new street address and phone number Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo Scientific 46360 Fremont Blvd Fremont, CA 94538, USA Ph: (510) 979-5000, ext 31819 or (800) 232-3342 ext 31819 Fax: (510) 979-5328 (800) 828-1628 (customer service) email: tim.morken@thermofisher.com The World Leader in Serving Science From jmcmillan <@t> ccpathology.com Tue Mar 24 12:43:16 2009 From: jmcmillan <@t> ccpathology.com (Jaime McMillan) Date: Tue Mar 24 12:43:22 2009 Subject: [Histonet] Reference or service lab that does PDGFR-a staining? In-Reply-To: References: Message-ID: USLabs performs this test. Jaime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, March 24, 2009 10:39 AM To: histonet Subject: [Histonet] Reference or service lab that does PDGFR-a staining? Hi, does anyone know of a reference or service lab that does immuno staining for PDGFR-a,b (platelet derived growth factor receptor alpha and/or beta. Please note my new street address and phone number Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo Scientific 46360 Fremont Blvd Fremont, CA 94538, USA Ph: (510) 979-5000, ext 31819 or (800) 232-3342 ext 31819 Fax: (510) 979-5328 (800) 828-1628 (customer service) email: tim.morken@thermofisher.com The World Leader in Serving Science _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Mar 24 13:40:49 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 24 13:40:55 2009 Subject: [Histonet] re: water In-Reply-To: <6abc767b0903230954l670a4684s8daa84e62ceb7234@mail.gmail.com> Message-ID: Does anyone know of a method to change the pH of tap water without going through a lot of expense? Possibly a filter to put directly on the water line. Thanks, Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Matthew Close Sent: Monday, March 23, 2009 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: water I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the tap source, I would say you are probably okay going with the distilled water. My word of caution is to check the level of air in your housing tank and never let it drop below half. Typically, the more air in the tank, the more CO2 going into the water which will gradually lower the pH down to 5.1-5.5 territory. Also, if you have the option to chose between using a glass carboy and a plastic one to store water, always go with the glass. While all of this has never been a real problem for me in terms of analyzable results, I often wonder if it has anything to do with why, sometimes, identical staining procedures on identially fixed tissues will stain notably different. Matt Close Department of Biological Sciences Lehigh University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From wlecorch <@t> rwjuhh.edu Tue Mar 24 14:13:49 2009 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Tue Mar 24 14:13:56 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 42 JCAHO - Universal Protocol (Amy Self) In-Reply-To: <1237913085.350983@messagescreen.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB7888C@HAMEXMBA.rwjham.local> We do 3 to 4 FNA's on a daily basis and no matter the site thyroid, liver ..Etc we go through the time out procedure every time, yes it may a little bit overkill for a palpable mass or using ultrasound guidance but none the less you must do it just like labeling the lido cane when it is the only syringe on the tray. -----Original Message----- ------------------------------ Message: 3 Date: Mon, 23 Mar 2009 14:10:35 -0400 From: "Amy Self" Subject: [Histonet] JCAHO - Universal Protocol To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Is there anyone out there that follows the JCAHO - Universal Protocol/Time Out procedure when the pathologist perform his/her own FNA's? We have a pathologist that will perform FNA's when asked and I was told that when he performs these procedures that we need to be following this universal protocol/time out procedure that is followed in the OR for surgeries. I have all the info. from the OR but to me the procedure that they follow is just too in-depth for the palpable masses that are aspirated for cytology. So I am turning to you all for help. I was just wondering what other facilities were doing about this. Any help would be appreciated. Thanks in advance. Amy Self Georgetown Hospital System From rgrow <@t> bmnet.com Tue Mar 24 14:14:40 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Mar 24 14:14:47 2009 Subject: [Histonet] LabVision line of instrumentation, antibodies and reagents Message-ID: I was wondering if there are any clinical labs that use the LabVision line of instrumentation, antibodies and reagents. We are a small hospital that does about 30 predilute antibodies and are considering changing from our current vendor/instrument. I would appreciate good, & bad experiences. I don't want to go into any change without knowing. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From matthewtclose <@t> gmail.com Tue Mar 24 14:32:02 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Tue Mar 24 14:32:10 2009 Subject: [Histonet] re:microtome questions Message-ID: <6abc767b0903241232l74bbd3bfuf0150eedf1a1f298@mail.gmail.com> I'd go for the old AO 820. They can be used by any and everyone and its nearly impossible to break them. From Lynn.Burton <@t> Illinois.gov Tue Mar 24 14:37:57 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Mar 24 14:38:56 2009 Subject: [Histonet] FW: formalin neutralizers References: Message-ID: Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: Burton, Lynn Sent: Tue 3/24/2009 10:31 AM To: histonet-bouces@lists.utsouthwestern.edu Subject: formalin neutralizers What is everyone using to neutral their formalin? Who has the best product for the price? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From NLinke <@t> mednet.ucla.edu Tue Mar 24 14:52:50 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Tue Mar 24 14:52:56 2009 Subject: [Histonet] LabVision line of instrumentation, antibodies and reagents In-Reply-To: Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE4451EEF52@EMGMB1.ad.medctr.ucla.edu> Hi Rene, I love LabVision products! Hopefully they won't be ruined by Fisher....(I would put in names of all acquisitions but it would take too long to type)... Noelle (LabVision cheerleader) No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rgrow@bmnet.com Sent: Tuesday, March 24, 2009 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LabVision line of instrumentation, antibodies and reagents I was wondering if there are any clinical labs that use the LabVision line of instrumentation, antibodies and reagents. We are a small hospital that does about 30 predilute antibodies and are considering changing from our current vendor/instrument. I would appreciate good, & bad experiences. I don't want to go into any change without knowing. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From Lizbeth_Kelly <@t> hgsi.com Tue Mar 24 15:05:57 2009 From: Lizbeth_Kelly <@t> hgsi.com (Lizbeth_Kelly@hgsi.com) Date: Tue Mar 24 15:06:12 2009 Subject: [Histonet] Re: CD31 anti-mouse on paraffin sections Message-ID: Hi, Has someone tried an antibody that would detect CD31 on mouse tissues using 10% NBF and not Zinc formalin? Thanks, Lizbeth Kelly, HT(ASCP), Q-IHC Histology Department Human Genome Sciences, Inc. 14200 Shady Grove Road Rockville, MD 20850 240-314-4400 X2325 Fax: 301-354-4176 From rjbuesa <@t> yahoo.com Tue Mar 24 15:17:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 24 15:17:57 2009 Subject: [Histonet] IF In-Reply-To: Message-ID: <146626.79881.qm@web65703.mail.ac4.yahoo.com> 30 min at room temperature Ren? J. --- On Tue, 3/24/09, Vanessa J. Phelan wrote: From: Vanessa J. Phelan Subject: [Histonet] IF To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 24, 2009, 12:17 PM Hi everyone, When carrying out IF on frozen sections how long after the cutting the sections should you leave the slides to dry and where before starting the staining procedure? Thanks a mill, V _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Mar 24 10:30:43 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 24 15:18:17 2009 Subject: [Histonet] hematoxylin Question In-Reply-To: <939251.95015.qm@web65713.mail.ac4.yahoo.com> Message-ID: We use ours for a few weeks, but we do filter it before use. kristen arvidson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/24/2009 07:48 AM Please respond to arvidsonkristen@yahoo.com To histonet cc Subject [Histonet] hematoxylin Question Hello All, How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is poured into a staining container? The stain gets used so infrequently I would like to keep it as long as I can. We currently dump it weekly regardless of use. Thank you. -Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Mar 23 23:27:11 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 24 15:19:15 2009 Subject: [Histonet] California Symposium Message-ID: The final program is available for the California Society for Histotechnology annual symposium. The symposium is scheduled for May 14-17 in Millbrae. If you are interested I can send you a word document or a pdf file. It will be posted on the CSH website in the next day or so. http://www.californiahistology.org/ Jennifer From fudo <@t> ufl.edu Tue Mar 24 15:39:15 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Mar 24 15:39:20 2009 Subject: [Histonet] QIHC certificate Message-ID: <102891417.107681237927155864.JavaMail.osg@osgjas03.cns.ufl.edu> Hi, all I have a question about how to QIHC certificate. We have several bilogical scientists work as full-time employee doing IHC staining. But none of them has HT certificate. In this case, can they get QIHC certificate? What kind of courses need they to take? Thank you, Ann Fu Lab Manager Dept. of Pathology University of Flodrida Gainesville, FL 32610 From detmar <@t> lunenfeld.ca Tue Mar 24 15:54:26 2009 From: detmar <@t> lunenfeld.ca (Jacqui Detmar) Date: Tue Mar 24 15:54:44 2009 Subject: [Histonet] questions re: fixing in general and Histochoice in particular Message-ID: Hi all. Having a bit of an internal debate here, so I would like to get the opinions of some of you in Histoland, please. Here are the questions: 1. When fixing with 10% NBF, for how long should you fix and what volume ratio of fixative:tissue should you use? 2. At what temperature should one be fixing tissues? Regarding Histochoice: 1. For how long should you fix the tissue? 2. What volume ratio of fixative:tissue should you use? 3. How long can you store Histochoice-fixed tissues in 70% ethanol? I think that's about it. Thanks in advance, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 25 Orde Street, room 6-1001AJ Toronto, ON M5T 3H7 Email: detmar@lunenfeld.ca Phone: 416-586-4800 x5607 Fax: 416-586-5993 From leiker <@t> buffalo.edu Tue Mar 24 16:15:19 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Mar 24 16:15:25 2009 Subject: [Histonet] questions re: fixing in general and Histochoice in particular In-Reply-To: References: Message-ID: <4DC242FF6EE1F71B7CA38E31@bchwxp2702.ad.med.buffalo.edu> 1. Generally 24 hrs for 2-3 mm thick piece. At least a 10:1 formalin:tissue volume. 2. Room temp or 4oC - I've heard debates, and I've heard it doesn't matter, but we do it at 4oC. --On Tuesday, March 24, 2009 4:54 PM -0400 Jacqui Detmar wrote: > Hi all. Having a bit of an internal debate here, so I would like to get > the opinions of some of you in Histoland, please. Here are the > questions: > > > > 1. When fixing with 10% NBF, for how long should you fix and what > volume ratio of fixative:tissue should you use? > > 2. At what temperature should one be fixing tissues? > > > > Regarding Histochoice: > > > > 1. For how long should you fix the tissue? > > 2. What volume ratio of fixative:tissue should you use? > > 3. How long can you store Histochoice-fixed tissues in 70% > ethanol? > > > > I think that's about it. Thanks in advance, > > > > Jacqui > > > > Jacqui Detmar, Post-doctoral Fellow > > Samuel Lunenfeld Research Institute > > Mount Sinai Hospital > > 25 Orde Street, room 6-1001AJ > > Toronto, ON M5T 3H7 > > > > Email: detmar@lunenfeld.ca > > Phone: 416-586-4800 x5607 > > Fax: 416-586-5993 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From cforster <@t> umn.edu Tue Mar 24 16:25:14 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Mar 24 16:25:01 2009 Subject: [Histonet] Looking for a CD20 that works on mouse tissue Message-ID: <49C94FBA.1070406@umn.edu> Hello Histonetters, Have a new project that wants to stain CD20 in mouse samples. One is brain and the other heart. Has anyone done this CD marker in mouse? if so, would you be willing to share with us! Thanks in advance, Colleen Forster U of MN From sally.norton <@t> seattlechildrens.org Tue Mar 24 16:52:07 2009 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Tue Mar 24 16:52:15 2009 Subject: [Histonet] LabVision line of instrumentation, antibodies and reagents In-Reply-To: References: Message-ID: <16E0693C7018C245959AC729FE66EDE52DBD4F@s107.childrens.sea.kids> Hello, We do IF's on renal biopsies and skin. We were getting our antibodies and Protein Block from ThermoShandon which is now Lab vision. We are now not getting our supplies when needed. We have Protein Block on order and were told we would not get any until end of April, which was then amended to the end of March. In the meantime we've run out and can't do a renal biopsy until we get some. Does anyone out there do IF on Renal's and who is your supplier. We are looking to find someone new. Thank you, Sally Norton, HT Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rgrow@bmnet.com Sent: Tuesday, March 24, 2009 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LabVision line of instrumentation,antibodies and reagents I was wondering if there are any clinical labs that use the LabVision line of instrumentation, antibodies and reagents. We are a small hospital that does about 30 predilute antibodies and are considering changing from our current vendor/instrument. I would appreciate good, & bad experiences. I don't want to go into any change without knowing. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Children's Hospital and Regional Medical Center is now Seattle Children's. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From katelin <@t> cuttingedgehistology.com Tue Mar 24 17:37:06 2009 From: katelin <@t> cuttingedgehistology.com (Katelin Lester) Date: Tue Mar 24 17:37:15 2009 Subject: [Histonet] IHC Stainer: Microm HMS 710i Message-ID: <1EA88E8222994CAB824E270C571DB640@Front> I am looking for help/advice for using our automated immunostainer the Microm HMS 710i .(Our user manual says Richard Allan Sci and the labels on the side say LabVision, we have calls into Thermo's LabVision department but they are unsure if they can assist us) Specifically, one of our problems is that some of our stains are producing dark coloring around the edges of the tissue. I am having issues troubleshooting and absolutely can not change any of the programming. I have been using this stainer independently for over a year, taught to use it by someone else who was taught by someone else. I am looking to change some programming as well as troubleshoot. Anyone who has experience with this stainer is welcome to contact me. Thank you, Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 From AnthonyH <@t> chw.edu.au Tue Mar 24 17:45:43 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 24 17:45:51 2009 Subject: [Histonet] FW: formalin neutralizers In-Reply-To: Message-ID: This is what we use: Neutralization and Disposal of Formalin Fixative 10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. The reaction with ammonia results in the formation of hexamethylenetetramine (commonly known as hexamine or methenamine). This can then be safely disposed of either as a liquid fertiliser or via the sewage system (check with your local authority). The reaction proceeds as follows: 6 CH2O + 4 NH3 ? C6H12N4 (Hexamethylenetetramine) + 6 H2O Procedure: 1. Before beginning, personnel must have the following safety equipment readily available in the event of an accidental spill: sorbent material (spill pillows, bulk sorbent) formaldehyde rated respirator. 2. Personnel must wear a lab coat or apron, safety goggles and neoprene gloves. 3. A pH meter or pH paper 4. To 1000 ml of 10% formalin (= 4% formaldehyde) add 56 ml of strong ammonia solution (27%). This will generate 31 g of hexamine (approximately a 3% solution). 5. Stir well. Reaction may produce heat. 6. Initially, the pH of the formaldehyde solution will be about 6. As ammonia is added and stirred, a fluffy white precipitate will result. Addition of sufficient ammonia will raise the pH to about 8. Because the neutralization of the formaldehyde requires less molecules of ammonia than the apparent acid-base reaction supplies hydronium ions, the pH change from acid to base is used as an indicator that an excess of ammonia has been added. 7. Let set overnight (12 hours). 8. The smell of formalin is greatly reduced or replaced by a faint whiff of ammonia. 9. Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If the "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized and you will need to add more ammonia. 10. Dispose of appropriately Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Wednesday, 25 March 2009 6:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: formalin neutralizers Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: Burton, Lynn Sent: Tue 3/24/2009 10:31 AM To: histonet-bouces@lists.utsouthwestern.edu Subject: formalin neutralizers What is everyone using to neutral their formalin? Who has the best product for the price? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lpwenk <@t> sbcglobal.net Tue Mar 24 19:47:43 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Mar 24 19:47:52 2009 Subject: [Histonet] QIHC certificate In-Reply-To: <102891417.107681237927155864.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: >From the ASCP Board of Registry webpage: www.ascp.org/bor Click on Qualifications Click on IHC There are 3 routes. To summarize, all experience must be within the last 5 years, in a US lab, Canadian lab, or CAP/JC accredited lab: 1. ASCP certified technologist + 6 months full time IHC experience 2. ASCP certified technian + 12 months full time IHC experience 3. BA/BS degree or higher from a regionally accredited college/university + 18 months full time IHC experience Full time acceptable experience must include: - Immunohistochemical and/or Immunofluorescence Preparations All of the following should have been performed by the applicant: - Staining technique - Selection of proper control material - Titration of immunologic reagents - Quality Assurance The applicant should have participated in Quality Assurance related to all of the following: - Specimen fixation, processing, microtomy - Reagent selection, preparation, storage, disposal - Method selection, validation, documentation - Quality control - Safety Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Tuesday, March 24, 2009 4:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC certificate Hi, all I have a question about how to QIHC certificate. We have several bilogical scientists work as full-time employee doing IHC staining. But none of them has HT certificate. In this case, can they get QIHC certificate? What kind of courses need they to take? Thank you, Ann Fu Lab Manager Dept. of Pathology University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Mar 25 04:48:16 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Wed Mar 25 04:48:33 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTG9va2luZyBmb3IgYSBDRDIwIHRoYXQgd29ya3Mgb24gbW91c2UgdGlzc3Vl?= References: <49C94FBA.1070406@umn.edu> Message-ID: <200903251748112574096@foxmail.com> ABCAM we r using ab9475 2009-03-25 TF ???? Colleen Forster ????? 2009-03-25 05:27:58 ???? Histonet ??? ??? [Histonet] Looking for a CD20 that works on mouse tissue Hello Histonetters, Have a new project that wants to stain CD20 in mouse samples. One is brain and the other heart. Has anyone done this CD marker in mouse? if so, would you be willing to share with us! Thanks in advance, Colleen Forster U of MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fudo <@t> ufl.edu Wed Mar 25 07:34:20 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Wed Mar 25 07:34:25 2009 Subject: [Histonet] QIHC certificate Message-ID: <1017168817.79271237984460588.JavaMail.osg@osgjas02.cns.ufl.edu> Thank you all for answering this question. I really appreciate. Have a nice day, Ann Fu On Tue Mar 24 20:47:43 EDT 2009, Lee & Peggy Wenk wrote: > From the ASCP Board of Registry webpage: > www.ascp.org/bor > Click on Qualifications > Click on IHC > > There are 3 routes. To summarize, all experience must be within > the last 5 > years, in a US lab, Canadian lab, or CAP/JC accredited lab: > 1. ASCP certified technologist + 6 months full time IHC > experience > 2. ASCP certified technian + 12 months full time IHC experience > 3. BA/BS degree or higher from a regionally accredited > college/university + > 18 months full time IHC experience > > Full time acceptable experience must include: > - Immunohistochemical and/or Immunofluorescence Preparations > All of the following should have been performed by the > applicant: - Staining technique - Selection of proper > control material - Titration of immunologic reagents - Quality > Assurance > The applicant should have participated in Quality Assurance > related to > all of the following: - Specimen fixation, processing, > microtomy - Reagent selection, preparation, storage, disposal > - Method selection, validation, documentation - Quality > control - Safety Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > FU,DONGTAO > Sent: Tuesday, March 24, 2009 4:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] QIHC certificate > > Hi, all > > I have a question about how to QIHC certificate. We have > several bilogical > scientists work as full-time employee doing IHC staining. But > none of them > has HT certificate. In this case, can they get QIHC certificate? > What kind > of courses need they to take? > > Thank you, > > Ann Fu Lab Manager > Dept. of Pathology > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From akbitting <@t> geisinger.edu Wed Mar 25 08:10:04 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Mar 25 08:10:25 2009 Subject: [Histonet] Histos-5 Message-ID: <49C9F4EC.2B7F.00C9.0@geisinger.edu> Anyone out there using the Histos-5 from Milestone? How much reagent does a Histomodule hold? I'm trying to figure out my return on investment using reagent savings. Do you really change your paraffin less often or is that just a sales pitch? How often do you change your paraffin and what is the volume of the paraffin tank? Thanks for your help once again, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From mcauliff <@t> umdnj.edu Wed Mar 25 08:35:32 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Mar 25 08:35:43 2009 Subject: [Histonet] questions re: fixing in general and Histochoice in particular In-Reply-To: References: Message-ID: <49CA3324.1070308@umdnj.edu> Jacqui Detmar wrote: > Hi all. Having a bit of an internal debate here, so I would like to get > the opinions of some of you in Histoland, please. Here are the > questions: > > > > 1. When fixing with 10% NBF, for how long should you fix and what > volume ratio of fixative:tissue should you use? > 48 hours is a minimum according to R.D. Lillie, one week is better. Really! Thickness of the tissue does not matter, formalin fixes slowly. Of course, if you are doing immuno you may need to sacrifice some fixation to retain antigenicity. Depends on the antigen. 10X the volume of the tissue. > 2. At what temperature should one be fixing tissues? > Room temperature. There is more than ample scientific evidence that fixing cold just slows down the already slow fixation process. > > > Regarding Histochoice: > I never use proprietary fixes, you don't know what is in them and the manufacturer can change the formulation any time he/she wants. > > > 1. For how long should you fix the tissue? > > 2. What volume ratio of fixative:tissue should you use? > > 3. How long can you store Histochoice-fixed tissues in 70% > ethanol? > > > > I think that's about it. Thanks in advance, > > > > Jacqui > > > > Jacqui Detmar, Post-doctoral Fellow > > Samuel Lunenfeld Research Institute > > Mount Sinai Hospital > > 25 Orde Street, room 6-1001AJ > > Toronto, ON M5T 3H7 > > > > Email: detmar@lunenfeld.ca > > Phone: 416-586-4800 x5607 > > Fax: 416-586-5993 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mike <@t> pathview.com Wed Mar 25 08:46:11 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Mar 25 08:47:09 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: References: Message-ID: <00c001c9ad50$13204280$3960c780$@com> Good morning, I was just at the Lab Infotech Summit in Las Vegas last week where the subject of the conference was informatics in Anatomic and Clinical Pathology. Along with the usual seminars were the usual vendors in the exhibitor's hall demonstrating and talking about their products and services. As one of those vendors, I had the opportunity to talk to a few people and a general trend appeared to merge -- one which I would like to dispel, if possible. I'd like to make sure that everyone is aware that you do NOT have to have middleware in order to have bar coded cassettes, slides, etc., and you do NOT have to have middleware in order to have specimen/material tracking. Let me explain. If, on the one hand, you are quite content with your current information system and you simply wish to add barcodes and specimen tracking and you do not want to work with your information system vendor because either they don't have this capability or for some other reason, then YES, middleware is a viable alternative. On the other hand, if you are planning to purchase a new Information System for your laboratory, then by all means, DEMAND of your new vendor, the ability to have barcoded everything and to have specimen tracking built into your new information system. There are lots of good reasons to have all this capability in your information system and not in some middleware product. I'd be happy to discuss the reasons for my statements, but I've taken up enough of everyone's time. If you'd like to hear more, then please, just ask. I just thought everyone should know... Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From asmith <@t> mail.barry.edu Wed Mar 25 08:46:22 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Mar 25 08:47:53 2009 Subject: [Histonet] Current books for Histotechnology In-Reply-To: References: Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcrawfor <@t> aerotek.com Wed Mar 25 08:53:21 2009 From: jcrawfor <@t> aerotek.com (Crawford, Jennifer) Date: Wed Mar 25 08:53:25 2009 Subject: [Histonet] Part Time Histotechnologist (Chicago, IL) Message-ID: <571A823E0300F549BE86279A872395770283FA5B@ag00-exmbx04.allegisgroup.com> Good morning! I currently have a part time Histotechnologist position available in the south suburbs of Chicago. The shifts are 10 hour days but only 1-3 days per week are required with occasional Saturdays. ASCP certification is required. Please contact me directly at jcrawfor@aerotek.com if you or someone you know is interested! Best regards, Jen Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From asmith <@t> mail.barry.edu Wed Mar 25 08:55:51 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Mar 25 08:57:20 2009 Subject: [Histonet] Current books for Histotechnology In-Reply-To: References: Message-ID: Definitely get the 4th edition of Kiernan. Also get the latest edition of Polak and van Noorden's INTRODUCTION TO IMMUNOCYTOCHEMISTRY. If you don't already have them, used second or third editions of Lillie's HISTOPATHOLOGIC TECHNIC AND PRACTICAL HISTOCHEMISTRY and Pierce's HISTOCHEMISTRY, THEORETICAL AND APPLIED are surprisingly useful. -Allen A. Smith,Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Mar 25 09:01:53 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Mar 25 09:03:26 2009 Subject: [Histonet] Current books for Histotechnology In-Reply-To: References: Message-ID: For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. -Allen a. Smith,Ph.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, March 23, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Current books for Histotechnology I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Mar 25 09:06:03 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Mar 25 09:06:07 2009 Subject: [Histonet] formaldehyde neutralizers Message-ID: Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee From rjbuesa <@t> yahoo.com Wed Mar 25 09:38:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 25 09:38:49 2009 Subject: [Histonet] formaldehyde neutralizers In-Reply-To: Message-ID: <925805.52259.qm@web65705.mail.ac4.yahoo.com> Me too! Ren? J. --- On Wed, 3/25/09, Robert Richmond wrote: From: Robert Richmond Subject: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 25, 2009, 10:06 AM Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Wed Mar 25 09:39:29 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Mar 25 09:39:40 2009 Subject: [Histonet] Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS! Message-ID: *Position of the Histology Supervisor* Do you want the benefits you deserve with the rewards you can see? Do you want the advancement, flexibility and resources to advance your career and provide the care that your patients need? **FREE medical and dental benefits, domestic partner benefits, excellent tuition reimbursement, continuing education and more!** *Description:* Supervise, coordinate and participate in providing laboratory services to meet the needs of patients as ordered by the medical staff and performed in accordance with defined standards and practices in general and unique to assigned section(s). *Day shift with variable start times - Monday - Friday* Bachelor of Science degree in Medical Technology or related biological science Six years of medical lab experience of which two years must be in particular section supervised. NYS licensure required HT, (ASCP) preferred *Montgomery**, **NY** area:* *60 miles from Danbury, CT* *60 miles from Newark, NJ* *60 miles from Scranton, PA* What a great opportunity! REMEMBER, THIS WON?T LAST. Interested? Please send resume in Microsoft Word format to: Alyssa@alliedsearchpartners.com **Please forward this email to anyone who you seems fit for this position, as the referral bonus for this position is $1000 if we place a person that you send to us in a position!** -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From gmartin <@t> marshallmedical.org Wed Mar 25 09:42:44 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Mar 25 09:42:50 2009 Subject: [Histonet] Frozen section Message-ID: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary From lamcna <@t> bpthosp.org Wed Mar 25 09:50:08 2009 From: lamcna <@t> bpthosp.org (McNabola, Angela) Date: Wed Mar 25 09:50:36 2009 Subject: [Histonet] Frozen section In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> Message-ID: <6FAFE015E5AC6B4ABAEF2883A6F8A6D9016C6393@EXCH1.bpthosp.org> We require a form for each specimen. Yes, the subsequent forms may have less information (i.e. procedure being performed), but all have the patient id information, sample type and description, etc. They are treated as separate samples. -Angela Angela McNabola, MS,HT(ASCP)SLS, QIHC Manager Histology/Cytology Department of Pathology Bridgeport Hospital 267 Grant Street Bridgeport, CT 06610 phone: 203-384-4434 lamcna@bpthosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Mar 25 09:55:13 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Mar 25 09:56:01 2009 Subject: [Histonet] Current books for Histotechnology In-Reply-To: References: Message-ID: <49CA45D1.4060903@umdnj.edu> A for atlases, I also like Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY. I don't like Ross and Pawlina's HISTOLOGY, the third edition by Ross, Romrell and Kaye is much better and might be cheaper. Geoff Smith, Allen wrote: > For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. > -Allen a. Smith,Ph.D. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire > Sent: Monday, March 23, 2009 11:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Current books for Histotechnology > > I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald > Sent: Fri 3/20/2009 3:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Current books for Histotechnology > > > > Our library has funds available to purchase books for the Histotechnology > program. The problem is that we need current books. We have the latest > Bancroft and Gamble. Any other suggestions for books that are newer than > 2000? I have suggested John Kiernan's latest. > By the way I did find a copy of Sheehan for $2,400!! > > Jennifer MacDonald > Education Coordinator, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From JWeems <@t> sjha.org Wed Mar 25 10:40:34 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 25 10:40:50 2009 Subject: [Histonet] Frozen section In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53EADC3@ITSSSXM01V6.one.ads.che.org> We require a separate req for each. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cbarone <@t> NEMOURS.ORG Wed Mar 25 10:46:03 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Mar 25 10:46:17 2009 Subject: [Histonet] RNA and DNA yields from Laser Microdissection Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE801471BC3@wlmmsx01.nemours.org> Histonet: I have a couple questions I would like to throw out to the experts: 1. Do you think higher yields of DNA /RNA in LMD are related to protocol, method differences from one instrument to another ( i.e. catapult verses,, gravity drop, verses, arcturus melt system...or more related to histologist v. research tech handling of the sample? 2. How do you feel about frozen verses paraffin as related to yield and quality in LMD/LCM? From nefff <@t> staff.uni-marburg.de Wed Mar 25 11:58:20 2009 From: nefff <@t> staff.uni-marburg.de (nefff@staff.uni-marburg.de) Date: Wed Mar 25 11:58:30 2009 Subject: [Histonet] RNA and DNA yields from Laser Microdissection In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE801471BC3@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE801471BC3@wlmmsx01.nemours.org> Message-ID: <20090325175820.h7xmiauajo48kgw0@home.staff.uni-marburg.de> Hi Carol! My experience is, the yield depends on the protocol you use. I've done the LCM system from arcturus and the PALM-System from Zeiss. The highest yields had been for RNA and DNA by using the Pico Pure Kit (MSD) while doing LCM. This had been frozen sections. We isolated DNA from FFPE-Tissue from PALM and LCM by a conventional Proteinase K digest and phenol extraction. There is a paper of Fend and Specht at the Am. J. of Pathology, 2002 or 2003 describing these protocols. As long as the histopathologist or the tech are able to identify the "cell/tissue" of interest, it doesn't influence the yield/quality of the RNA/DNA. Frauke Zitat von "Barone, Carol " : > Histonet: I have a couple questions I would like to throw out to the experts: > > 1. Do you think higher yields of DNA /RNA in LMD are related to > protocol, method differences from one instrument to another ( i.e. > catapult verses,, gravity drop, verses, > arcturus melt system...or more related to histologist v. research > tech handling of the sample? > > 2. How do you feel about frozen verses paraffin as related to yield > and quality in LMD/LCM? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From NMargaryan <@t> childrensmemorial.org Wed Mar 25 12:03:22 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Mar 25 12:03:46 2009 Subject: [Histonet] LabVision line of instrumentation, antibodies In-Reply-To: References: Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601E6D2DF@CMHEXC01EVS.childrensmemorial.org> I love l kind of LabVision products! They are clear and the best! Good luck, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL? 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rgrow@bmnet.com Sent: Tuesday, March 24, 2009 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LabVision line of instrumentation, antibodies and reagents I was wondering if there are any clinical labs that use the LabVision line of instrumentation, antibodies and reagents. We are a small hospital that does about 30 predilute antibodies and are considering changing from our current vendor/instrument. I would appreciate good, & bad experiences. I don't want to go into any change without knowing. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax From RSRICHMOND <@t> aol.com Wed Mar 25 12:50:57 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Mar 25 12:51:00 2009 Subject: [Histonet] Re: Frozen section Message-ID: Somewhere in the USA Gary Martin asks: >>We need to make a change in the way we presently account for our frozen section [specimens] while doing [the frozen sections]. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections.<< Well, somewhere in the frozen section process the pathologist, who presumably knows what the specimen is, has to record a diagnosis on paper. That diagnosis should certainly include the anatomic site (which I suppose is your problem). Thus I might write: 3. skin of nose (re-excision of 3 to 6 o'clock margin): no basal cell carcinoma seen. I think you need to ask your pathologists to help you with this problem. Bob Richmond Samurai Pathologist Knoxville TN From Rcartun <@t> harthosp.org Wed Mar 25 14:07:38 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Mar 25 14:07:50 2009 Subject: [Histonet] INI-1 IHC Message-ID: <49CA48BA020000770000A631@gwmail4.harthosp.org> For those of you who do (or want to do) IHC staining for INI-1 (used to diagnose malignant rhabdoid tumor), Cell Marque (Rocklin, CA) now has a mouse mAb that is labeled "IVD" that works beautifully on formalin-fixed, paraffin-embedded tissue. We are still conducting our validation, but preliminary results look very promising. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From matthewtclose <@t> gmail.com Wed Mar 25 14:22:00 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Wed Mar 25 14:22:03 2009 Subject: [Histonet] re: water pH Message-ID: <6abc767b0903251222g3587a613i983bf2ee5dea0591@mail.gmail.com> I don't know that there's a way to change the pH of water through filtration. The pH is affected mostly by dissolved gasses and ions which aren't easily removed through filtration alone. You can adjust the pH by using buffered solutions, but then its really not water anymore. If you just have bad tap water, I am not sure the cost of RO but I am fairly sure its drastically cheaper than a large-scale distilltion setup and might give you water of sufficient quality. I would say the absolute best solution for good H20 is distillation. It doesn't have to be an incredibly fancy setup, and would depend largely on your demand. You may even have luck locating some used equipment for the job in the case that you need to generate lots of it. And if your lab is broke most of the time like mine, for small quantities, you could just use a simple DIY setup. The still has been around for centuries and really hasn't changed drastically in terms of design and basic function. Either way I think it would be worth the investment. -Matt From jcampbell <@t> vdxpathology.com Wed Mar 25 14:52:04 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Wed Mar 25 14:52:09 2009 Subject: [Histonet] Opinions on the Labvision 360 autostainer? Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF81DF65B@VDXSERVER01.vdxpathology.local> Hi everyone, Has anyone used the Labvision 360 autostainer? Any good or bad things about it? Is it suitable for IHC on animal tissues? Trying to sort out which stainer will work best for us and which is the most economical of course. I appreciate any feedback. Please no inquiries from sales reps. Thanks Jen C. From Harris <@t> medinst.com Wed Mar 25 14:58:05 2009 From: Harris <@t> medinst.com (Corliss Harris) Date: Wed Mar 25 14:58:17 2009 Subject: [Histonet] Plastic Thin Sections Won't Stay Message-ID: <49CA548E.9DCF.0062.0@medinst.com> We are re-visiting thin sectioning of MMA embedded items. The sections are great, but none of them stay on the slides for the staining. We're trying Sta-On, and have tried chrome alum slides. We use a butoxy mixture to stretch them and immuno quality slides. Any ideas? Thanks for your help! -- Corliss Harris Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-463-7537 ext. 1150 765-497-0641-fax From Wanda.Smith <@t> HCAhealthcare.com Wed Mar 25 15:53:08 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Mar 25 15:53:26 2009 Subject: [Histonet] RE: Frozen section In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BAE82B10BE@NADCWPMSGCMS03.hca.corpad.net> Gary, We require an additional requisition with additional specimens labeled and numbered correctly in sequential order. In other words, if a second and third specimen is sent, the 2nd requisition will mark the #1 specimen as "Previously sent for FS" and the additional specimens will be listed in the #2, #3, spot on the requisition. Does that make sense? Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbruce <@t> vetpathservicesinc.com Wed Mar 25 15:53:25 2009 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Wed Mar 25 15:53:35 2009 Subject: [Histonet] RE:guide to special stains Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A28551B9@vpss1.VetPathServicesInc.local> http://www.dako.com/prod_productrelatedinformation?url=literature_guides.htm You can also email them and request the guide. Or you can download the PDF, but it is VERY long, too much to print out. Does anyone have or know where to obtain a list of special stains that >briefly describes which each one is for? > > > >Thanks, > > > >Patricia Zerfas > >National Institutes of Health > >Building 28A, Room 112 > >28 Library Drive > >Bethesda, MD 20892 > >ph: (301) 496-4464 > >fax: (301) 402-1068 -- Does anyone have or know where to obtain a list of special stains that >briefly describes which each one is for? > > > >Thanks, > > > >Patricia Zerfas > >National Institutes of Health > >Building 28A, Room 112 > >28 Library Drive > >Bethesda, MD 20892 > >ph: (301) 496-4464 > >fax: (301) 402-1068 -- Does anyone have or know where to obtain a list of special stains that >briefly describes which each one is for? > > > >Thanks, > > > >Patricia Zerfas > >National Institutes of Health > >Building 28A, Room 112 > >28 Library Drive > >Bethesda, MD 20892 > >ph: (301) 496-4464 > >fax: (301) 402-1068 -- _______________________________ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbruce@vetpathservicesinc.com www.vetpathservicesinc.com From claycal44 <@t> yahoo.com Wed Mar 25 16:15:57 2009 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Wed Mar 25 16:16:01 2009 Subject: [Histonet] Collagen 111 Message-ID: <104043.71552.qm@web65615.mail.ac4.yahoo.com> I was wondering if anyone has done Collagen 111 antibody IHC on FFPE mouse tissue?(Bone)? If so, and you got good results, could you please let me know where the Antibody is from, and also the protocol you used.? High heat for antigen retreival is not working well for the bone tissue , and I have got weak results trying Hyaluronidase, Prot. K, and Trypsin. Any help would be appreciated. Thanks, Nancy Lowen claycal44@yahoo.net Research From cbarone <@t> NEMOURS.ORG Wed Mar 25 16:37:45 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Mar 25 16:37:54 2009 Subject: [Histonet] RE: Histonet Digest, Vol 64, Issue 44 In-Reply-To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE801471BCD@wlmmsx01.nemours.org> 4-12 hours dependent on sample size (a nickel if a good reference)...volume 1:20 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 25, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: questions re: fixing in general and Histochoice in particular (Geoff McAuliffe) 2. Information Systems: Specimen Tracking & MiddleWare (Michael Mihalik) 3. RE: Current books for Histotechnology (Smith, Allen) 4. Part Time Histotechnologist (Chicago, IL) (Crawford, Jennifer) 5. RE: Current books for Histotechnology (Smith, Allen) 6. RE: Current books for Histotechnology (Smith, Allen) 7. formaldehyde neutralizers (Robert Richmond) 8. Re: formaldehyde neutralizers (Rene J Buesa) 9. Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS! (Alyssa Peterson) 10. Frozen section (Martin, Gary) 11. RE: Frozen section (McNabola, Angela) 12. Re: Current books for Histotechnology (Geoff McAuliffe) 13. RE: Frozen section (Weems, Joyce) 14. RNA and DNA yields from Laser Microdissection (Barone, Carol ) 15. Re: RNA and DNA yields from Laser Microdissection (nefff@staff.uni-marburg.de) ---------------------------------------------------------------------- Message: 1 Date: Wed, 25 Mar 2009 09:35:32 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] questions re: fixing in general and Histochoice in particular To: Jacqui Detmar Cc: histonet@lists.utsouthwestern.edu Message-ID: <49CA3324.1070308@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Jacqui Detmar wrote: > Hi all. Having a bit of an internal debate here, so I would like to get > the opinions of some of you in Histoland, please. Here are the > questions: > > > > 1. When fixing with 10% NBF, for how long should you fix and what > volume ratio of fixative:tissue should you use? > 48 hours is a minimum according to R.D. Lillie, one week is better. Really! Thickness of the tissue does not matter, formalin fixes slowly. Of course, if you are doing immuno you may need to sacrifice some fixation to retain antigenicity. Depends on the antigen. 10X the volume of the tissue. > 2. At what temperature should one be fixing tissues? > Room temperature. There is more than ample scientific evidence that fixing cold just slows down the already slow fixation process. > > > Regarding Histochoice: > I never use proprietary fixes, you don't know what is in them and the manufacturer can change the formulation any time he/she wants. > > > 1. For how long should you fix the tissue? > > 2. What volume ratio of fixative:tissue should you use? > > 3. How long can you store Histochoice-fixed tissues in 70% > ethanol? > > > > I think that's about it. Thanks in advance, > > > > Jacqui > > > > Jacqui Detmar, Post-doctoral Fellow > > Samuel Lunenfeld Research Institute > > Mount Sinai Hospital > > 25 Orde Street, room 6-1001AJ > > Toronto, ON M5T 3H7 > > > > Email: detmar@lunenfeld.ca > > Phone: 416-586-4800 x5607 > > Fax: 416-586-5993 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 2 Date: Wed, 25 Mar 2009 08:46:11 -0500 From: "Michael Mihalik" Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare To: Message-ID: <00c001c9ad50$13204280$3960c780$@com> Content-Type: text/plain; charset="US-ASCII" Good morning, I was just at the Lab Infotech Summit in Las Vegas last week where the subject of the conference was informatics in Anatomic and Clinical Pathology. Along with the usual seminars were the usual vendors in the exhibitor's hall demonstrating and talking about their products and services. As one of those vendors, I had the opportunity to talk to a few people and a general trend appeared to merge -- one which I would like to dispel, if possible. I'd like to make sure that everyone is aware that you do NOT have to have middleware in order to have bar coded cassettes, slides, etc., and you do NOT have to have middleware in order to have specimen/material tracking. Let me explain. If, on the one hand, you are quite content with your current information system and you simply wish to add barcodes and specimen tracking and you do not want to work with your information system vendor because either they don't have this capability or for some other reason, then YES, middleware is a viable alternative. On the other hand, if you are planning to purchase a new Information System for your laboratory, then by all means, DEMAND of your new vendor, the ability to have barcoded everything and to have specimen tracking built into your new information system. There are lots of good reasons to have all this capability in your information system and not in some middleware product. I'd be happy to discuss the reasons for my statements, but I've taken up enough of everyone's time. If you'd like to hear more, then please, just ask. I just thought everyone should know... Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 ------------------------------ Message: 3 Date: Wed, 25 Mar 2009 09:46:22 -0400 From: "Smith, Allen" Subject: RE: [Histonet] Current books for Histotechnology To: 'Jennifer MacDonald' Cc: "'Histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 25 Mar 2009 09:53:21 -0400 From: "Crawford, Jennifer" Subject: [Histonet] Part Time Histotechnologist (Chicago, IL) To: Message-ID: <571A823E0300F549BE86279A872395770283FA5B@ag00-exmbx04.allegisgroup.com> Content-Type: text/plain; charset="us-ascii" Good morning! I currently have a part time Histotechnologist position available in the south suburbs of Chicago. The shifts are 10 hour days but only 1-3 days per week are required with occasional Saturdays. ASCP certification is required. Please contact me directly at jcrawfor@aerotek.com if you or someone you know is interested! Best regards, Jen Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. ------------------------------ Message: 5 Date: Wed, 25 Mar 2009 09:55:51 -0400 From: "Smith, Allen" Subject: RE: [Histonet] Current books for Histotechnology To: 'Jennifer MacDonald' Cc: "'Histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Definitely get the 4th edition of Kiernan. Also get the latest edition of Polak and van Noorden's INTRODUCTION TO IMMUNOCYTOCHEMISTRY. If you don't already have them, used second or third editions of Lillie's HISTOPATHOLOGIC TECHNIC AND PRACTICAL HISTOCHEMISTRY and Pierce's HISTOCHEMISTRY, THEORETICAL AND APPLIED are surprisingly useful. -Allen A. Smith,Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 25 Mar 2009 10:01:53 -0400 From: "Smith, Allen" Subject: RE: [Histonet] Current books for Histotechnology To: 'Ingles Claire ' Cc: "'Histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. -Allen a. Smith,Ph.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, March 23, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Current books for Histotechnology I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 25 Mar 2009 10:06:03 -0400 From: Robert Richmond Subject: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee ------------------------------ Message: 8 Date: Wed, 25 Mar 2009 07:38:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu, Robert Richmond Message-ID: <925805.52259.qm@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Me too! Ren? J. --- On Wed, 3/25/09, Robert Richmond wrote: From: Robert Richmond Subject: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 25, 2009, 10:06 AM Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 25 Mar 2009 10:39:29 -0400 From: Alyssa Peterson Subject: [Histonet] Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS! To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 *Position of the Histology Supervisor* Do you want the benefits you deserve with the rewards you can see? Do you want the advancement, flexibility and resources to advance your career and provide the care that your patients need? **FREE medical and dental benefits, domestic partner benefits, excellent tuition reimbursement, continuing education and more!** *Description:* Supervise, coordinate and participate in providing laboratory services to meet the needs of patients as ordered by the medical staff and performed in accordance with defined standards and practices in general and unique to assigned section(s). *Day shift with variable start times - Monday - Friday* Bachelor of Science degree in Medical Technology or related biological science Six years of medical lab experience of which two years must be in particular section supervised. NYS licensure required HT, (ASCP) preferred *Montgomery**, **NY** area:* *60 miles from Danbury, CT* *60 miles from Newark, NJ* *60 miles from Scranton, PA* What a great opportunity! REMEMBER, THIS WON'T LAST. Interested? Please send resume in Microsoft Word format to: Alyssa@alliedsearchpartners.com **Please forward this email to anyone who you seems fit for this position, as the referral bonus for this position is $1000 if we place a person that you send to us in a position!** -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ------------------------------ Message: 10 Date: Wed, 25 Mar 2009 07:42:44 -0700 From: "Martin, Gary" Subject: [Histonet] Frozen section To: Message-ID: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary ------------------------------ Message: 11 Date: Wed, 25 Mar 2009 10:50:08 -0400 From: "McNabola, Angela" Subject: RE: [Histonet] Frozen section To: "Martin, Gary" , Message-ID: <6FAFE015E5AC6B4ABAEF2883A6F8A6D9016C6393@EXCH1.bpthosp.org> Content-Type: text/plain; charset="us-ascii" We require a form for each specimen. Yes, the subsequent forms may have less information (i.e. procedure being performed), but all have the patient id information, sample type and description, etc. They are treated as separate samples. -Angela Angela McNabola, MS,HT(ASCP)SLS, QIHC Manager Histology/Cytology Department of Pathology Bridgeport Hospital 267 Grant Street Bridgeport, CT 06610 phone: 203-384-4434 lamcna@bpthosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 25 Mar 2009 10:55:13 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] Current books for Histotechnology Cc: "'Histonet@lists.utsouthwestern.edu'" , 'Ingles Claire ' Message-ID: <49CA45D1.4060903@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed A for atlases, I also like Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY. I don't like Ross and Pawlina's HISTOLOGY, the third edition by Ross, Romrell and Kaye is much better and might be cheaper. Geoff Smith, Allen wrote: > For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. > -Allen a. Smith,Ph.D. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire > Sent: Monday, March 23, 2009 11:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Current books for Histotechnology > > I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald > Sent: Fri 3/20/2009 3:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Current books for Histotechnology > > > > Our library has funds available to purchase books for the Histotechnology > program. The problem is that we need current books. We have the latest > Bancroft and Gamble. Any other suggestions for books that are newer than > 2000? I have suggested John Kiernan's latest. > By the way I did find a copy of Sheehan for $2,400!! > > Jennifer MacDonald > Education Coordinator, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 13 Date: Wed, 25 Mar 2009 11:40:34 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Frozen section To: "Martin, Gary" , Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53EADC3@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We require a separate req for each. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 14 Date: Wed, 25 Mar 2009 11:46:03 -0400 From: "Barone, Carol " Subject: [Histonet] RNA and DNA yields from Laser Microdissection To: histonet@lists.utsouthwestern.edu Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE801471BC3@wlmmsx01.nemours.org> Content-Type: text/plain; charset=iso-8859-1 Histonet: I have a couple questions I would like to throw out to the experts: 1. Do you think higher yields of DNA /RNA in LMD are related to protocol, method differences from one instrument to another ( i.e. catapult verses,, gravity drop, verses, arcturus melt system...or more related to histologist v. research tech handling of the sample? 2. How do you feel about frozen verses paraffin as related to yield and quality in LMD/LCM? ------------------------------ Message: 15 Date: Wed, 25 Mar 2009 17:58:20 +0100 From: nefff@staff.uni-marburg.de Subject: Re: [Histonet] RNA and DNA yields from Laser Microdissection To: "Barone, Carol " Cc: histonet@lists.utsouthwestern.edu Message-ID: <20090325175820.h7xmiauajo48kgw0@home.staff.uni-marburg.de> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; format="flowed" Hi Carol! My experience is, the yield depends on the protocol you use. I've done the LCM system from arcturus and the PALM-System from Zeiss. The highest yields had been for RNA and DNA by using the Pico Pure Kit (MSD) while doing LCM. This had been frozen sections. We isolated DNA from FFPE-Tissue from PALM and LCM by a conventional Proteinase K digest and phenol extraction. There is a paper of Fend and Specht at the Am. J. of Pathology, 2002 or 2003 describing these protocols. As long as the histopathologist or the tech are able to identify the "cell/tissue" of interest, it doesn't influence the yield/quality of the RNA/DNA. Frauke Zitat von "Barone, Carol " : > Histonet: I have a couple questions I would like to throw out to the experts: > > 1. Do you think higher yields of DNA /RNA in LMD are related to > protocol, method differences from one instrument to another ( i.e. > catapult verses,, gravity drop, verses, > arcturus melt system...or more related to histologist v. research > tech handling of the sample? > > 2. How do you feel about frozen verses paraffin as related to yield > and quality in LMD/LCM? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 64, Issue 44 **************************************** From ratliffjack <@t> hotmail.com Wed Mar 25 16:54:34 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Mar 25 16:54:39 2009 Subject: [Histonet] Plastic Thin Sections Won't Stay In-Reply-To: <49CA548E.9DCF.0062.0@medinst.com> References: <49CA548E.9DCF.0062.0@medinst.com> Message-ID: Try coating your slides with Haupt's adhesive. It can be purchased commercially from Fisher (or you can make it yourself using glycerin, gelatin, and phenol) and then cut it 1:1 with 50% EtOH to prepare a working dilution. I have used Haupt's for many years on both small (1x3 slides) and large (2x3 slides) and have NEVER lost a section (knocking on wood now...LOL) to staining. Make sure to look over your slides before use to insure adequate coating of the slide. Please keep in mind that with every new thing you bring into the lab, you should do some testing of your own. Specifically, you will want to research the concentrate that works best for your staining as it does yield to some degree of background when staining with hematoxylin, fast green, and aniline blue if the concentration is too high. Jack Ratliff > Date: Wed, 25 Mar 2009 15:58:05 -0400 > From: Harris@medinst.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Plastic Thin Sections Won't Stay > > We are re-visiting thin sectioning of MMA embedded items. The sections are great, but none of them stay on the slides for the staining. We're trying Sta-On, and have tried chrome alum slides. We use a butoxy mixture to stretch them and immuno quality slides. Any ideas? > Thanks for your help! > > -- > > Corliss Harris > Histology Technician > MED Institute > 1 Geddes Way > West Lafayette, IN 47906 > 765-463-7537 ext. 1150 > 765-497-0641-fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Mar 25 17:06:35 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Mar 25 17:08:58 2009 Subject: [Histonet] formaldehyde neutralizers In-Reply-To: Message-ID: Bob, Thanks for that. I am not sure how the bisulphite method works. I picked it up from a reference on formalin neutralisation but have never tried it. And would you believe that after some searching I can't even find that reference (I probably have it on my home computer). I have a suspician that the details may even have come from John Kiernan. The notes come from my "Infamous" text book I have been writing for the last 20 years. As my staff call it, the book that will probably never be published. But then the chapters are quite usefull for teaching so they are of some use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, 26 March 2009 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formaldehyde neutralizers Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From becky.garrison <@t> jax.ufl.edu Wed Mar 25 17:31:31 2009 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Wed Mar 25 17:31:36 2009 Subject: [Histonet] Frozen section In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C04DD56D4@mailsvr.MARSHMED.local> Message-ID: We require our OR to submit a separate requisition with each container. The specimen site is listed on each requisition. This applies to all specimens (not just frozens). In addition, frozens (and other intra operative consultations such as gross only or touch prep) must also have a "frozen section form" for each container. The frozen section form has patient ID, clinical history and specimen site fields completed by the OR and a diagnosis field which is completed and signed by the pathologist. Original goes to patient chart; copy stays in Pathology. When a specimen is submitted for frozen, all 3 must have matching information: container, requisition and frozen section form. If discrepant, OR must fix immediately before the frozen is signed out. Any later specimen, even on same patient, must have a separate requisition and (if applicable) a frozen form. It is the responsibility of the OR to accurately identify the specimen site. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Wed Mar 25 17:29:34 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Mar 25 18:03:31 2009 Subject: [Histonet] Formalin and Solvent Recyclers Message-ID: <73A7ED895EE0C24D9267ED814911DF190E13B44E@exchange.cmc-nh.org> I'm looking for solvent and formalin recyclers that have a fairly small footprint and fairly straight forward ventilation requirements. Any suggestions or critiques of recyclers. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From joelleweaver <@t> hotmail.com Thu Mar 26 05:09:13 2009 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 26 05:09:24 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: <00c001c9ad50$13204280$3960c780$@com> References: <00c001c9ad50$13204280$3960c780$@com> Message-ID: Thanks for posting this.I just couldn't help commenting on this post because as a working histologist, I have tried to convince managers in the past who have tried to recituify the need for specimen tracking in histology, and the general situation with very time consuming, tedious and inaccurate manual transcription steps in the effort to create tracking and not have to buy anything. I have printed off information from vendor websites, showing that middleware was not always needed, and presented this information to them, but they just don't believe it. Lack of understanding I think, caused them to instead go with manual the manual processes to create a "paper trail". Even in this day and age, people are surprisingly afraid of, and unaware of technology. In my experience, these manual processes are marginally effective at best, and of course moved the process away from efficiency and reliability, not to mention frustrated employees who are already struggling to get their work done with time pressures and staffing constraints. My position has always been that computer systems are very good at some things, such as repetitive information processing, and they do not get tired, transpose numbers etc. Please use them for this!You cannot check a process step which introduces humam errors of oversight and transcription with another process that introduces the same type of error potential. To do so, merely expands the opportunity for this kind of error to pass farther into the process. As a working histologist, I do wish that people would not be so afraid of technology in our field, and use it to improve and update histology processes.More education is needed! So keep posting this type of information. Only by incorporating this aspect will the field be able to move forward and keep pace with the other areas of the laboratory and medical practice in general. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 25 Mar 2009 08:46:11 -0500 > Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > Good morning, > > I was just at the Lab Infotech Summit in Las Vegas last week where the > subject of the conference was informatics in Anatomic and Clinical > Pathology. Along with the usual seminars were the usual vendors in the > exhibitor's hall demonstrating and talking about their products and > services. > > As one of those vendors, I had the opportunity to talk to a few people and a > general trend appeared to merge -- one which I would like to dispel, if > possible. > > I'd like to make sure that everyone is aware that you do NOT have to have > middleware in order to have bar coded cassettes, slides, etc., and you do > NOT have to have middleware in order to have specimen/material tracking. > Let me explain. > > If, on the one hand, you are quite content with your current information > system and you simply wish to add barcodes and specimen tracking and you do > not want to work with your information system vendor because either they > don't have this capability or for some other reason, then YES, middleware is > a viable alternative. > > On the other hand, if you are planning to purchase a new Information System > for your laboratory, then by all means, DEMAND of your new vendor, the > ability to have barcoded everything and to have specimen tracking built into > your new information system. There are lots of good reasons to have all > this capability in your information system and not in some middleware > product. I'd be happy to discuss the reasons for my statements, but I've > taken up enough of everyone's time. If you'd like to hear more, then > please, just ask. > > I just thought everyone should know... > > > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A From rjbuesa <@t> yahoo.com Thu Mar 26 08:02:12 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 26 08:02:19 2009 Subject: [Histonet] Formalin and Solvent Recyclers In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190E13B44E@exchange.cmc-nh.org> Message-ID: <705075.85665.qm@web65708.mail.ac4.yahoo.com> Stephen: I used during many years a BR recycler for xylene. ? As for formalin I really encourage you to think it twice before trying to recycle formalin because:? 1- if you unfortunately decide to go for a formalin condensation recycler the exposure to formalin will happen in?5 opportunities: emptying the containers in the recycler, recycling, eliminating the?waste, adding the salts to neutralize it, including pH checking,?and while?bottling it again.? 2- if you use a filtration "purifier" you will be exposed in 3?opportunities: emptying the containers in the recycler, testing the pH after the filtration, and bottling it again. Formalin is such a dangerous substance that no matter how much you "save" is not worth it because you cannot put a price to the health of the operator. As to the "green" sales pitch of the recyclers salespersons, the correct?action will be to make sure that those who dispose of it under contract neutralize it adequately. Formalin is something that unfortunately is "here to stay" (as has been for the last 100 years) and the correct action with it is to avoid its exposure as much as possible. Think it twice before embarking on a formalin recycling program. Ren? J. --- On Wed, 3/25/09, Feher, Stephen wrote: From: Feher, Stephen Subject: [Histonet] Formalin and Solvent Recyclers To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 25, 2009, 6:29 PM I'm looking for solvent and formalin recyclers that have a fairly small footprint and fairly straight forward ventilation requirements. Any suggestions or critiques of recyclers. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Mar 26 08:08:32 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Mar 26 08:09:04 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: References: <00c001c9ad50$13204280$3960c780$@com> Message-ID: <015d01c9ae13$fc97fac0$f5c7f040$@com> Quote: You cannot check a process step which introduces human errors of oversight and transcription with another process that introduces the same type of error potential. I love that line. May I use it? The only thing I would add is a subtlety. The easiest way to use barcoding in any information system is to just 'add it on'. A truly efficient system INCORPORATES the technology. What do I mean? Here's an example: In Scenario 1: bar coded cassettes are printing at accessioning. They are then moved to the gross area with the requisitions and specimen. However, we know that cassettes can be separated from the requisitions and specimen, so some systems have you scan the specimen and each block to confirm that they match. This is an example of an 'add on' functionality. The additional step to scan the specimen and blocks has been added. This increases quality at the cost of more work. In Scenario 2: bar coded cassettes are printed at the grossing station by scanning the bar code on the specimen label. Only the blocks for that specimen are printed. This provides the same increase in quality WITHOUT any extra work. This is an example of an 'incorporated' technology. The difference between the two philosophies is huge and it's a hard one to ferret out by simply reading product brochures because in both scenarios 'barcodes are used'. .but you have to ask yourself, which system would you use? And finally, I apologize if this is coming across as a sales pitch, but I'm very, very passionate about work flow analysis. The best systems out there don't just collect information, they help you get your work done faster, better, etc. and you can't do that without analyzing how work flows to the department, within the department, and out of the department. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, March 26, 2009 5:09 AM To: mike@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Thanks for posting this.I just couldn't help commenting on this post because as a working histologist, I have tried to convince managers in the past who have tried to recituify the need for specimen tracking in histology, and the general situation with very time consuming, tedious and inaccurate manual transcription steps in the effort to create tracking and not have to buy anything. I have printed off information from vendor websites, showing that middleware was not always needed, and presented this information to them, but they just don't believe it. Lack of understanding I think, caused them to instead go with manual the manual processes to create a "paper trail". Even in this day and age, people are surprisingly afraid of, and unaware of technology. In my experience, these manual processes are marginally effective at best, and of course moved the process away from efficiency and reliability, not to mention frustrated employees who are already struggling to get their work done with time pressures and staffing constraints. My position has always been that computer systems are very good at some things, such as repetitive information processing, and they do not get tired, transpose numbers etc. Please use them for this!You cannot check a process step which introduces humam errors of oversight and transcription with another process that introduces the same type of error potential. To do so, merely expands the opportunity for this kind of error to pass farther into the process. As a working histologist, I do wish that people would not be so afraid of technology in our field, and use it to improve and update histology processes.More education is needed! So keep posting this type of information. Only by incorporating this aspect will the field be able to move forward and keep pace with the other areas of the laboratory and medical practice in general. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 25 Mar 2009 08:46:11 -0500 > Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > Good morning, > > I was just at the Lab Infotech Summit in Las Vegas last week where the > subject of the conference was informatics in Anatomic and Clinical > Pathology. Along with the usual seminars were the usual vendors in the > exhibitor's hall demonstrating and talking about their products and > services. > > As one of those vendors, I had the opportunity to talk to a few people and a > general trend appeared to merge -- one which I would like to dispel, if > possible. > > I'd like to make sure that everyone is aware that you do NOT have to have > middleware in order to have bar coded cassettes, slides, etc., and you do > NOT have to have middleware in order to have specimen/material tracking. > Let me explain. > > If, on the one hand, you are quite content with your current information > system and you simply wish to add barcodes and specimen tracking and you do > not want to work with your information system vendor because either they > don't have this capability or for some other reason, then YES, middleware is > a viable alternative. > > On the other hand, if you are planning to purchase a new Information System > for your laboratory, then by all means, DEMAND of your new vendor, the > ability to have barcoded everything and to have specimen tracking built into > your new information system. There are lots of good reasons to have all > this capability in your information system and not in some middleware > product. I'd be happy to discuss the reasons for my statements, but I've > taken up enough of everyone's time. If you'd like to hear more, then > please, just ask. > > I just thought everyone should know... > > > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Mar 26 08:28:16 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Mar 26 08:28:21 2009 Subject: [Histonet] INI-1 IHC In-Reply-To: <49CA48BA020000770000A631@gwmail4.harthosp.org> References: <49CA48BA020000770000A631@gwmail4.harthosp.org> Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A0B596F@mailbe01.mc.vanderbilt.edu> I agree with Richard, I absolutely LOVE this antibody! If you're using one of the RUO antibodies that require special retrieval, etc... Try this one! It's consistent and should easily fit into your current system. We've used it to make the diagnosis on a few (very difficult) suspected AT/RT pedi neuro tumors! No, I do not in any way receive compensation from Cell Marque :) Have a great day, everyone! Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Wednesday, March 25, 2009 2:08 PM To: Histonet Subject: [Histonet] INI-1 IHC For those of you who do (or want to do) IHC staining for INI-1 (used to diagnose malignant rhabdoid tumor), Cell Marque (Rocklin, CA) now has a mouse mAb that is labeled "IVD" that works beautifully on formalin-fixed, paraffin-embedded tissue. We are still conducting our validation, but preliminary results look very promising. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From litepath2000 <@t> yahoo.com Thu Mar 26 08:33:49 2009 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Thu Mar 26 08:33:55 2009 Subject: [Histonet] Reminder: New York State Histotechnological Society Annual Meeting April 24 & 25 2009 Fishkill, NY Message-ID: <991965.63652.qm@web58803.mail.re1.yahoo.com> This is a reminder that the New York State Histotechnological Society Annual Meeting is coming?up on 24th?and 25th of April. The symposium will be held at the Holiday Inn in Fishkill, New York (845-896-6281 or www.hifishkill.com )? Our academic program offers over 25 contact hours of CEU's on a wide range of topics. For a copy of the program, registration forms or additional information,?please visit the New York State Histotechnological Society website?at: www.nyhisto.org?.? Please feel free to distribute. ?------- New York State Histotechnological Society NYSHS Website www.nyhisto.org NYSHS Message Board http://tech.groups.yahoo.com/group/NYSHS1972/? From tp2 <@t> medicine.wisc.edu Thu Mar 26 08:40:53 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu Mar 26 08:41:13 2009 Subject: [Histonet] Opinions on the Labvision 360 autostainer? Message-ID: <49CB3F95020000DF000167F0@gwmail.medicine.wisc.edu> I have been very happy with the Autostainer 360. It's great in a research lab, because it's a totally open system. The programming is fairly intuitive. Tom Pier >>> "Jennifer Campbell" 03/25/09 2:56 PM >>> Hi everyone, Has anyone used the Labvision 360 autostainer? Any good or bad things about it? Is it suitable for IHC on animal tissues? Trying to sort out which stainer will work best for us and which is the most economical of course. I appreciate any feedback. Please no inquiries from sales reps. Thanks Jen C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Thu Mar 26 09:12:44 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Mar 26 09:27:13 2009 Subject: [Histonet] PCR question Message-ID: <61135F0455D33347B5AAE209B903A30429D34D3E@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this hoping that you can pass this along to colleagues that work in molecular labs doing PCR testing: Does anyone who performs either T-cell or B-cell rearrangements use the kits/reagents from InVivoScribe Technologies? Have you been contacted by InVivoScribe Technologies to pay a royalty, licensing fee and bi-annual assay fees? Martha Ward, Assistant Manager Molecular Diagnostics Lab Wake Forest University Baptist Medical Center From victor <@t> pathology.washington.edu Thu Mar 26 09:38:43 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Mar 26 09:38:49 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: <015d01c9ae13$fc97fac0$f5c7f040$@com> References: <00c001c9ad50$13204280$3960c780$@com> <015d01c9ae13$fc97fac0$f5c7f040$@com> Message-ID: <49CB9373.8090209@pathology.washington.edu> If anyone is seriously looking at a new Lab Information System LIS, please give Michael a call. This is not a paid endorsement. We do not use his product, but being only a few miles away from where they went live with their first client, has given us the opportunity to see the program. We use PowerPath currently, but if we were in a shopping mood, Michael's product Pathview would be on the short list. Michael and our team have met several times and his philosophies and ours' are so in sync it is scary. We have developed our own software to incorporate with PowerPath. Cases that need only one cassette are printed at accessioning. For complex cases the PA or Resident orders the cassettes in real time. They scan the specimen label and select the quantity of cassettes. This was a major change in workflow for us, which had it's challenges. Now that the bugs are out of the system, the end users really like it. No more wasting cassettes because too many were printed. The cassettes are scanned at embedding to provide information or special instructions to the embedder. Scanning the cassette at the microtome generates the slide labels. Scanning the slide after staining marks the order as completed and starts the tracking of the slide to the pathologist and back to the slide room. Enough rambling, the technology is available and getting better all the time. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Michael Mihalik wrote: > Quote: You cannot check a process step which introduces human errors of > oversight and transcription with another process that introduces the same > type of error potential. > > > > I love that line. May I use it? > > > > > > > > The only thing I would add is a subtlety. The easiest way to use barcoding > in any information system is to just 'add it on'. A truly efficient system > INCORPORATES the technology. What do I mean? Here's an example: > > > > > > In Scenario 1: bar coded cassettes are printing at accessioning. They are > then moved to the gross area with the requisitions and specimen. However, > we know that cassettes can be separated from the requisitions and specimen, > so some systems have you scan the specimen and each block to confirm that > they match. This is an example of an 'add on' functionality. The > additional step to scan the specimen and blocks has been added. This > increases quality at the cost of more work. > > > > In Scenario 2: bar coded cassettes are printed at the grossing station by > scanning the bar code on the specimen label. Only the blocks for that > specimen are printed. This provides the same increase in quality WITHOUT > any extra work. This is an example of an 'incorporated' technology. > > > > The difference between the two philosophies is huge and it's a hard one to > ferret out by simply reading product brochures because in both scenarios > 'barcodes are used'. > > > > .but you have to ask yourself, which system would you use? > > > > And finally, I apologize if this is coming across as a sales pitch, but I'm > very, very passionate about work flow analysis. The best systems out there > don't just collect information, they help you get your work done faster, > better, etc. and you can't do that without analyzing how work flows to the > department, within the department, and out of the department. > > > > > > Michael Mihalik > > PathView Systems | cell: 214.733.7688 | > 800.798.3540 | fax: 270.423.0968 > > > > > > > > From: joelle weaver [mailto:joelleweaver@hotmail.com] > Sent: Thursday, March 26, 2009 5:09 AM > To: mike@pathview.com; Histonet > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > > > Thanks for posting this.I just couldn't help commenting on this post because > as a working histologist, I have tried to convince managers in the past who > have tried to recituify the need for specimen tracking in histology, and the > general situation with very time consuming, tedious and inaccurate manual > transcription steps in the effort to create tracking and not have to buy > anything. I have printed off information from vendor websites, showing that > middleware was not always needed, and presented this information to them, > but they just don't believe it. Lack of understanding I think, caused them > to instead go with manual the manual processes to create a "paper trail". > Even in this day and age, people are surprisingly afraid of, and unaware of > technology. In my experience, these manual processes are marginally > effective at best, and of course moved the process away from efficiency and > reliability, not to mention frustrated employees who are already struggling > to get their work done with time pressures and staffing constraints. My > position has always been that computer systems are very good at some things, > such as repetitive information processing, and they do not get tired, > transpose numbers etc. Please use them for this!You cannot check a process > step which introduces humam errors of oversight and transcription with > another process that introduces the same type of error potential. To do so, > merely expands the opportunity for this kind of error to pass farther into > the process. As a working histologist, I do wish that people would not be so > afraid of technology in our field, and use it to improve and update > histology processes.More education is needed! So keep posting this type of > information. Only by incorporating this aspect will the field be able to > move forward and keep pace with the other areas of the laboratory and > medical practice in general. > > Joelle Weaver HTL (ASCP) > > >> From: mike@pathview.com >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 25 Mar 2009 08:46:11 -0500 >> Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare >> >> Good morning, >> >> I was just at the Lab Infotech Summit in Las Vegas last week where the >> subject of the conference was informatics in Anatomic and Clinical >> Pathology. Along with the usual seminars were the usual vendors in the >> exhibitor's hall demonstrating and talking about their products and >> services. >> >> As one of those vendors, I had the opportunity to talk to a few people and >> > a > >> general trend appeared to merge -- one which I would like to dispel, if >> possible. >> >> I'd like to make sure that everyone is aware that you do NOT have to have >> middleware in order to have bar coded cassettes, slides, etc., and you do >> NOT have to have middleware in order to have specimen/material tracking. >> Let me explain. >> >> If, on the one hand, you are quite content with your current information >> system and you simply wish to add barcodes and specimen tracking and you >> > do > >> not want to work with your information system vendor because either they >> don't have this capability or for some other reason, then YES, middleware >> > is > >> a viable alternative. >> >> On the other hand, if you are planning to purchase a new Information >> > System > >> for your laboratory, then by all means, DEMAND of your new vendor, the >> ability to have barcoded everything and to have specimen tracking built >> > into > >> your new information system. There are lots of good reasons to have all >> this capability in your information system and not in some middleware >> product. I'd be happy to discuss the reasons for my statements, but I've >> taken up enough of everyone's time. If you'd like to hear more, then >> please, just ask. >> >> I just thought everyone should know... >> >> >> >> >> Michael Mihalik >> PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _____ > > Quick access to Windows Live and your favorite MSN content with Internet > Explorer 8. > N55C0701A> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbraud <@t> holyredeemer.com Thu Mar 26 09:54:13 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Mar 26 09:54:21 2009 Subject: [Histonet] RE: Frozen Sections In-Reply-To: <25336ef6000045e2@HolyRedeemer.com> Message-ID: Gary - Our institution follows the exact procedure listed by Wanda, copied below. - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax "We require an additional requisition with additional specimens labeled and numbered correctly in sequential order. In other words, if a second and third specimen is sent, the 2nd requisition will mark the #1 specimen as "Previously sent for FS" and the additional specimens will be listed in the #2, #3, spot on the requisition. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From sheila_adey <@t> hotmail.com Thu Mar 26 10:21:47 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Mar 26 10:21:51 2009 Subject: [Histonet] Dako Rep, please call Message-ID: Could the Dako Rep for Port Huron Michigan give me a call? 810-987-5000 ext. 2128 Thanks Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Chat with the whole group, and bring everyone together. http://go.microsoft.com/?linkid=9650735 From litepath2000 <@t> yahoo.com Thu Mar 26 10:29:12 2009 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Thu Mar 26 10:29:16 2009 Subject: [Histonet] Update: Reminder: New York State Histotechnological Society Annual Meeting April 24 & 25 2009 Fishkill, NY Message-ID: <460889.21481.qm@web58805.mail.re1.yahoo.com> The deadline for room reservations with the reduced rate is tommorow, 3/27/09 Be sure and use the room block code: ?HIS? or mention the New York Histotechnological Society to receive the special meeting room rate of: Single/Double: $99.00/night. ----- Forwarded Message ---- From: NYSHisto To: Histonet List Sent: Thursday, March 26, 2009 9:33:49 AM Subject: Reminder: New York State Histotechnological Society Annual Meeting April 24 & 25 2009 Fishkill, NY This is a reminder that the New York State Histotechnological Society Annual Meeting is coming?up on 24th?and 25th of April. The symposium will be held at the Holiday Inn in Fishkill, New York (845-896-6281 or www.hifishkill.com )? Our academic program offers over 25 contact hours of CEU's on a wide range of topics. For a copy of the program, registration forms or additional information,?please visit the New York State Histotechnological Society website?at: www.nyhisto.org?.? Please feel free to distribute. ?------- New York State Histotechnological Society NYSHS Website www.nyhisto.org NYSHS Message Board http://tech.groups.yahoo.com/group/NYSHS1972/? From kenneth.metzger <@t> aruplab.com Thu Mar 26 11:18:01 2009 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Mar 26 11:18:08 2009 Subject: [Histonet] Whole Mount Immunos Message-ID: One of my pathologist would like to do PSA, P504S (alpha-methylacyl-CoA racemase), and CD31 on whole mount prostates. Is there anyone out there doing this and if so could I ask some questions of you? We have not done immunos on whole mounts before and since no automation supports whole mount slides (we have Ventana and Dako) I would have to go back to manual...which I haven't done in a LONG time. Recommend any kits, protocols, ect...Any help would be appreciated. Thanks, Ken PS Thanks for the help getting the charged whole mount slides from my earlier email. Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From janderson <@t> halozyme.com Thu Mar 26 11:18:53 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Thu Mar 26 11:19:02 2009 Subject: [Histonet] Please help! In dire need of user manuals Message-ID: Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From jdhisto <@t> yahoo.com Thu Mar 26 11:39:03 2009 From: jdhisto <@t> yahoo.com (Jonathan De La Rosa) Date: Thu Mar 26 11:39:08 2009 Subject: [Histonet] Tissue Tek VIP 2000 Message-ID: <40048.22230.qm@web110713.mail.gq1.yahoo.com> Im looking for a "Tissue Tek VIP 2000" manual. Could anyone help me out? I went to the?archives and could not find in particular a "manual" hiding in there anywhere. Any and all help is appreciated. ? JD?? ? From dlschneider <@t> gmail.com Thu Mar 26 11:41:31 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Thu Mar 26 11:41:36 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: References: Message-ID: <1085e7000903260941w4db45e6w8f50b06d64ec223b@mail.gmail.com> Have you asked Sakura, Leica, and Microm? It would be interesting if they wouldn't provide manuals for their equipment, used or not. On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson wrote: > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of this > message to any person other than the intended recipient(s) is not intended > in any way to waive confidentiality or any applicable privilege. Any > review, retransmission, dissemination or other use of, or taking of any > action in reliance upon, this information by individuals or entities other > than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please contact > the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mpence <@t> grhs.net Thu Mar 26 11:58:50 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Mar 26 11:58:56 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <1085e7000903260941w4db45e6w8f50b06d64ec223b@mail.gmail.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3AF2@IS-E2K3.grhs.net> I'm sure each of them will sell you a manual. So much for business relations! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Thursday, March 26, 2009 11:42 AM To: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals Have you asked Sakura, Leica, and Microm? It would be interesting if they wouldn't provide manuals for their equipment, used or not. On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson wrote: > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these > instruments came with a user's manual. I am in need of a manual > (hard-copy or PDF) for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of > someone. It takes a lot of time to copy a 50-page manual. I'll repay > the favor, if at all possible. I'm hoping that a vendor may raise > their hand and offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is > intended only for the person(s) or entity to which it is addressed. > Delivery of this message to any person other than the intended > recipient(s) is not intended in any way to waive confidentiality or > any applicable privilege. Any review, retransmission, dissemination > or other use of, or taking of any action in reliance upon, this > information by individuals or entities other than the intended > recipient is prohibited by Halozyme and may be in violation of > applicable laws. If you received this in error, please contact the > sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Thu Mar 26 12:02:08 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Mar 26 12:02:21 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <1085e7000903260941w4db45e6w8f50b06d64ec223b@mail.gmail.com> References: <1085e7000903260941w4db45e6w8f50b06d64ec223b@mail.gmail.com> Message-ID: <49CBB510.2040904@umdnj.edu> since the vip 3000 was dinscontinued sometime late last century, id say finding a manual from its vendor is gonna be hard... http://www.sakura-americas.com/techsupport/VIP1000.html Daniel Schneider wrote: > Have you asked Sakura, Leica, and Microm? > > It would be interesting if they wouldn't provide manuals for their > equipment, used or not. > > > On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson > wrote: > > >> Hello Everyone. >> >> >> >> I our lab we've purchased all used equipment. None of these instruments >> came with a user's manual. I am in need of a manual (hard-copy or PDF) >> for the following: >> >> >> >> Sakura Tissue-Tek VIP 3000 >> >> Leica Jung Histo Embedder >> >> Microm HM335E Microtome >> >> >> >> I do realize that requesting a copy of these is a lot to ask of someone. >> It takes a lot of time to copy a 50-page manual. I'll repay the favor, >> if at all possible. I'm hoping that a vendor may raise their hand and >> offer a copy? I won't hold my breath for that... >> >> >> >> Thanks everyone. >> >> >> >> Jennifer M. Anderson, Scientist >> >> Halozyme Therapeutics, Inc. >> >> 11404 Sorrento Valley Road >> >> San Diego, CA 92121 >> >> 858-704-8333 >> >> janderson@halozyme.com >> >> >> >> >> >> >> The information transmitted in this email is confidential and is intended >> only for the person(s) or entity to which it is addressed. Delivery of this >> message to any person other than the intended recipient(s) is not intended >> in any way to waive confidentiality or any applicable privilege. Any >> review, retransmission, dissemination or other use of, or taking of any >> action in reliance upon, this information by individuals or entities other >> than the intended recipient is prohibited by Halozyme and may be in >> violation of applicable laws. If you received this in error, please contact >> the sender and delete/destroy this email. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From cathy <@t> wasatchhisto.com Thu Mar 26 12:10:43 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Thu Mar 26 12:11:10 2009 Subject: [Histonet] Isocut cutting fluid Message-ID: <444F2F3748DC4B27A12357847763C2CB@shop1e2e996aa5> Free to good home 6 bottles of 950 ml Buehler Isocut cutting fluid This was sent to us accidently by Buehler and we never used Just pay shipping and it is all yours Cathy Mayton Wasatch Histo Consultants, Inc. From trathborne <@t> somerset-healthcare.com Thu Mar 26 12:19:55 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 26 12:20:01 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <49CBB510.2040904@umdnj.edu> Message-ID: I have a service manual for the VIP 3000. Contact me off line. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Peter Carroll Sent: Thursday, March 26, 2009 1:02 PM To: Daniel Schneider Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals since the vip 3000 was dinscontinued sometime late last century, id say finding a manual from its vendor is gonna be hard... http://www.sakura-americas.com/techsupport/VIP1000.html Daniel Schneider wrote: > Have you asked Sakura, Leica, and Microm? > > It would be interesting if they wouldn't provide manuals for their > equipment, used or not. > > > On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson > wrote: > > >> Hello Everyone. >> >> >> >> I our lab we've purchased all used equipment. None of these instruments >> came with a user's manual. I am in need of a manual (hard-copy or PDF) >> for the following: >> >> >> >> Sakura Tissue-Tek VIP 3000 >> >> Leica Jung Histo Embedder >> >> Microm HM335E Microtome >> >> >> >> I do realize that requesting a copy of these is a lot to ask of someone. >> It takes a lot of time to copy a 50-page manual. I'll repay the favor, >> if at all possible. I'm hoping that a vendor may raise their hand and >> offer a copy? I won't hold my breath for that... >> >> >> >> Thanks everyone. >> >> >> >> Jennifer M. Anderson, Scientist >> >> Halozyme Therapeutics, Inc. >> >> 11404 Sorrento Valley Road >> >> San Diego, CA 92121 >> >> 858-704-8333 >> >> janderson@halozyme.com >> >> >> >> >> >> >> The information transmitted in this email is confidential and is intended >> only for the person(s) or entity to which it is addressed. Delivery of this >> message to any person other than the intended recipient(s) is not intended >> in any way to waive confidentiality or any applicable privilege. Any >> review, retransmission, dissemination or other use of, or taking of any >> action in reliance upon, this information by individuals or entities other >> than the intended recipient is prohibited by Halozyme and may be in >> violation of applicable laws. If you received this in error, please contact >> the sender and delete/destroy this email. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From alyssa <@t> alliedsearchpartners.com Thu Mar 26 13:04:12 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Mar 26 13:04:17 2009 Subject: [Histonet] Lead Histotech Position In NY Message-ID: *Position of the Lead Histotech* ** *Description:* Monday-Friday Day Shift Position Full Time Permanent, with full medical benefits Needs to be a seasoned veteran in histology capible of working on your own. Location: Montgomery, NY area Private Anatomical Pathology Laboratory Interested? Please send resume in Microsoft Word format to: Alyssa@alliedsearchpartners.com **Please forward this email to anyone who you seems fit for this position, as the referral bonus for this position is $1000 if we place a person that you send to us in a position!** -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From vjp2105 <@t> columbia.edu Thu Mar 26 13:23:49 2009 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Thu Mar 26 13:23:55 2009 Subject: [Histonet] Commercial Eosin Message-ID: Hi everyone, Has anyone had the experience of massive overstaining with commercial Surgipath Alcohol-based Eosin? I manually stained with Surgipath Hx for 2mins, then bluer the eosin for 30secs and the whole tissue was completely pink! Thanks V From PMEarle <@t> CapeCodHealth.org Thu Mar 26 13:58:01 2009 From: PMEarle <@t> CapeCodHealth.org (Earle, Paul M.) Date: Thu Mar 26 14:13:12 2009 Subject: [Histonet] Please help! In dire need of user manuals (Jennifer Anderson) In-Reply-To: <200903261700.n2QH0QEt010867@mail> References: <200903261700.n2QH0QEt010867@mail> Message-ID: <29688FCA0750AD4FB55B18D961EB91CC04AABEA9@cchex2.cchdomain1.capecodhealth.org> Jennifer, I just mailed a copy to Kevin Maxson yesterday (Wednesday) at his request Paul M. Earle M.S., P.A. (ASCP) Anatomic Pathology Supervisor Pathology Department Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 508-457-5699 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 26, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 46 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From janderson <@t> halozyme.com Thu Mar 26 15:23:32 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Thu Mar 26 15:23:38 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3AF2@IS-E2K3.grhs.net> References: <1085e7000903260941w4db45e6w8f50b06d64ec223b@mail.gmail.com> <661949901A768E4F9CC16D8AF8F2838C017A3AF2@IS-E2K3.grhs.net> Message-ID: WOW WOW WOW WOW WOW! I need to thank you all whole-heartedly for the outpouring of help! There are so many of you who have come to my rescue! So THANK YOU THANK YOU THANK YOU SO VERY MUCH! Jen Anderson Halozyme Therapeutics, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, March 26, 2009 9:59 AM To: Daniel Schneider; Histonet Subject: RE: [Histonet] Please help! In dire need of user manuals I'm sure each of them will sell you a manual. So much for business relations! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Thursday, March 26, 2009 11:42 AM To: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals Have you asked Sakura, Leica, and Microm? It would be interesting if they wouldn't provide manuals for their equipment, used or not. On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson wrote: > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these > instruments came with a user's manual. I am in need of a manual > (hard-copy or PDF) for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of > someone. It takes a lot of time to copy a 50-page manual. I'll repay > the favor, if at all possible. I'm hoping that a vendor may raise > their hand and offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is > intended only for the person(s) or entity to which it is addressed. > Delivery of this message to any person other than the intended > recipient(s) is not intended in any way to waive confidentiality or > any applicable privilege. Any review, retransmission, dissemination > or other use of, or taking of any action in reliance upon, this > information by individuals or entities other than the intended > recipient is prohibited by Halozyme and may be in violation of > applicable laws. If you received this in error, please contact the > sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Mar 26 15:52:27 2009 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 26 15:52:33 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: <015d01c9ae13$fc97fac0$f5c7f040$@com> References: <00c001c9ad50$13204280$3960c780$@com> <015d01c9ae13$fc97fac0$f5c7f040$@com> Message-ID: I am very passionate about this as well! Yes, you may "use" my line- but if you could, and it fits into the conversation, indicate that it was histotech that said it! I completely agree that the best use of technology, such as bar coding is to incorporate it.- and especially true in light of the histology process which is "peppered" if you will, with intensly manual steps. Our field will never catch up in advancement until more people accept this notion. So what you are saying really does not come across as a "pitch" to me, because I think it just makes sense. Regards- Joelle Weaver HTL (ASCP) From: mike@pathview.com To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Date: Thu, 26 Mar 2009 08:08:32 -0500 Quote: You cannot check a process step which introduces human errors of oversight and transcription with another process that introduces the same type of error potential. I love that line. May I use it? The only thing I would add is a subtlety. The easiest way to use barcoding in any information system is to just ?add it on?. A truly efficient system INCORPORATES the technology. What do I mean? Here?s an example: In Scenario 1: bar coded cassettes are printing at accessioning. They are then moved to the gross area with the requisitions and specimen. However, we know that cassettes can be separated from the requisitions and specimen, so some systems have you scan the specimen and each block to confirm that they match. This is an example of an ?add on? functionality. The additional step to scan the specimen and blocks has been added. This increases quality at the cost of more work. In Scenario 2: bar coded cassettes are printed at the grossing station by scanning the bar code on the specimen label. Only the blocks for that specimen are printed. This provides the same increase in quality WITHOUT any extra work. This is an example of an ?incorporated? technology. The difference between the two philosophies is huge and it?s a hard one to ferret out by simply reading product brochures because in both scenarios ?barcodes are used?. ?but you have to ask yourself, which system would you use? And finally, I apologize if this is coming across as a sales pitch, but I?m very, very passionate about work flow analysis. The best systems out there don?t just collect information, they help you get your work done faster, better, etc. and you can?t do that without analyzing how work flows to the department, within the department, and out of the department. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, March 26, 2009 5:09 AM To: mike@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Thanks for posting this.I just couldn't help commenting on this post because as a working histologist, I have tried to convince managers in the past who have tried to recituify the need for specimen tracking in histology, and the general situation with very time consuming, tedious and inaccurate manual transcription steps in the effort to create tracking and not have to buy anything. I have printed off information from vendor websites, showing that middleware was not always needed, and presented this information to them, but they just don't believe it. Lack of understanding I think, caused them to instead go with manual the manual processes to create a "paper trail". Even in this day and age, people are surprisingly afraid of, and unaware of technology. In my experience, these manual processes are marginally effective at best, and of course moved the process away from efficiency and reliability, not to mention frustrated employees who are already struggling to get their work done with time pressures and staffing constraints. My position has always been that computer systems are very good at some things, such as repetitive information processing, and they do not get tired, transpose numbers etc. Please use them for this!You cannot check a process step which introduces humam errors of oversight and transcription with another process that introduces the same type of error potential. To do so, merely expands the opportunity for this kind of error to pass farther into the process. As a working histologist, I do wish that people would not be so afraid of technology in our field, and use it to improve and update histology processes.More education is needed! So keep posting this type of information. Only by incorporating this aspect will the field be able to move forward and keep pace with the other areas of the laboratory and medical practice in general. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 25 Mar 2009 08:46:11 -0500 > Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > Good morning, > > I was just at the Lab Infotech Summit in Las Vegas last week where the > subject of the conference was informatics in Anatomic and Clinical > Pathology. Along with the usual seminars were the usual vendors in the > exhibitor's hall demonstrating and talking about their products and > services. > > As one of those vendors, I had the opportunity to talk to a few people and a > general trend appeared to merge -- one which I would like to dispel, if > possible. > > I'd like to make sure that everyone is aware that you do NOT have to have > middleware in order to have bar coded cassettes, slides, etc., and you do > NOT have to have middleware in order to have specimen/material tracking. > Let me explain. > > If, on the one hand, you are quite content with your current information > system and you simply wish to add barcodes and specimen tracking and you do > not want to work with your information system vendor because either they > don't have this capability or for some other reason, then YES, middleware is > a viable alternative. > > On the other hand, if you are planning to purchase a new Information System > for your laboratory, then by all means, DEMAND of your new vendor, the > ability to have barcoded everything and to have specimen tracking built into > your new information system. There are lots of good reasons to have all > this capability in your information system and not in some middleware > product. I'd be happy to discuss the reasons for my statements, but I've > taken up enough of everyone's time. If you'd like to hear more, then > please, just ask. > > I just thought everyone should know... > > > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From pumla.pamla <@t> gmail.com Thu Mar 26 16:25:07 2009 From: pumla.pamla <@t> gmail.com (Pumla Pamla-Gutter) Date: Thu Mar 26 16:25:10 2009 Subject: [Histonet] Glycerol jelly with an anti-fading agent? Message-ID: Does anyone know if it is OK to add an anti-fading agent such n-propyl gallate to glycerol jelly? I know it is ok to add it to buffered glycerol, but the problem with just using the buffered glycerol, is that it does not solidy and so my tissue sections(250umthick sections) are at risk of drying out. The way we make our glycerol jelly is with gelatin powder, water, and glycerol. Are there any adverse reactions with the gelatin and the anti-fading agent? Thanks, Pumla Pamla-Gutter Ohio State University 305W 12th Ave. Columbus, OH, 43232 (614) 292-4046 From mike <@t> pathview.com Thu Mar 26 16:44:16 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Mar 26 16:46:06 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: References: <00c001c9ad50$13204280$3960c780$@com> <015d01c9ae13$fc97fac0$f5c7f040$@com> Message-ID: <00f501c9ae5c$0ef94050$2cebc0f0$@com> Thanks and no problem with your request. I'm a big one for 2 things: 1. Admit when you make a mistake, and 2. Always give credit where credit is due. .and along those lines, the best part of our system, comes from the people who work in AP, day in and day out. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, March 26, 2009 3:52 PM To: mike@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare I am very passionate about this as well! Yes, you may "use" my line- but if you could, and it fits into the conversation, indicate that it was histotech that said it! I completely agree that the best use of technology, such as bar coding is to incorporate it.- and especially true in light of the histology process which is "peppered" if you will, with intensly manual steps. Our field will never catch up in advancement until more people accept this notion. So what you are saying really does not come across as a "pitch" to me, because I think it just makes sense. Regards- Joelle Weaver HTL (ASCP) _____ From: mike@pathview.com To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Date: Thu, 26 Mar 2009 08:08:32 -0500 Quote: You cannot check a process step which introduces human errors of oversight and transcription with another process that introduces the same type of error potential. I love that line. May I use it? The only thing I would add is a subtlety. The easiest way to use barcoding in any information system is to just 'add it on'. A truly efficient system INCORPORATES the technology. What do I mean? Here's an example: In Scenario 1: bar coded cassettes are printing at accessioning. They are then moved to the gross area with the requisitions and specimen. However, we know that cassettes can be separated from the requisitions and specimen, so some systems have you scan the specimen and each block to confirm that they match. This is an example of an 'add on' functionality. The additional step to scan the specimen and blocks has been added. This increases quality at the cost of more work. In Scenario 2: bar coded cassettes are printed at the grossing station by scanning the bar code on the specimen label. Only the blocks for that specimen are printed. This provides the same increase in quality WITHOUT any extra work. This is an example of an 'incorporated' technology. The difference between the two philosophies is huge and it's a hard one to ferret out by simply reading product brochures because in both scenarios 'barcodes are used'. .but you have to ask yourself, which system would you use? And finally, I apologize if this is coming across as a sales pitch, but I'm very, very passionate about work flow analysis. The best systems out there don't just collect information, they help you get your work done faster, better, etc. and you can't do that without analyzing how work flows to the department, within the department, and out of the department. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, March 26, 2009 5:09 AM To: mike@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Thanks for posting this.I just couldn't help commenting on this post because as a working histologist, I have tried to convince managers in the past who have tried to recituify the need for specimen tracking in histology, and the general situation with very time consuming, tedious and inaccurate manual transcription steps in the effort to create tracking and not have to buy anything. I have printed off information from vendor websites, showing that middleware was not always needed, and presented this information to them, but they just don't believe it. Lack of understanding I think, caused them to instead go with manual the manual processes to create a "paper trail". Even in this day and age, people are surprisingly afraid of, and unaware of technology. In my experience, these manual processes are marginally effective at best, and of course moved the process away from efficiency and reliability, not to mention frustrated employees who are already struggling to get their work done with time pressures and staffing constraints. My position has always been that computer systems are very good at some things, such as repetitive information processing, and they do not get tired, transpose numbers etc. Please use them for this!You cannot check a process step which introduces humam errors of oversight and transcription with another process that introduces the same type of error potential. To do so, merely expands the opportunity for this kind of error to pass farther into the process. As a working histologist, I do wish that people would not be so afraid of technology in our field, and use it to improve and update histology processes.More education is needed! So keep posting this type of information. Only by incorporating this aspect will the field be able to move forward and keep pace with the other areas of the laboratory and medical practice in general. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 25 Mar 2009 08:46:11 -0500 > Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > Good morning, > > I was just at the Lab Infotech Summit in Las Vegas last week where the > subject of the conference was informatics in Anatomic and Clinical > Pathology. Along with the usual seminars were the usual vendors in the > exhibitor's hall demonstrating and talking about their products and > services. > > As one of those vendors, I had the opportunity to talk to a few people and a > general trend appeared to merge -- one which I would like to dispel, if > possible. > > I'd like to make sure that everyone is aware that you do NOT have to have > middleware in order to have bar coded cassettes, slides, etc., and you do > NOT have to have middleware in order to have specimen/material tracking. > Let me explain. > > If, on the one hand, you are quite content with your current information > system and you simply wish to add barcodes and specimen tracking and you do > not want to work with your information system vendor because either they > don't have this capability or for some other reason, then YES, middleware is > a viable alternative. > > On the other hand, if you are planning to purchase a new Information System > for your laboratory, then by all means, DEMAND of your new vendor, the > ability to have barcoded everything and to have specimen tracking built into > your new information system. There are lots of good reasons to have all > this capability in your information system and not in some middleware > product. I'd be happy to discuss the reasons for my statements, but I've > taken up enough of everyone's time. If you'd like to hear more, then > please, just ask. > > I just thought everyone should know... > > > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. _____ HotmailR is up to 70% faster. Now good news travels really fast. Find out more. From macveigh <@t> usc.edu Thu Mar 26 16:52:04 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Mar 26 16:51:46 2009 Subject: [Histonet] Glicerol jelly with anti-fading agent Message-ID: <002201c9ae5d$19b08480$5c237d80@DFS66DD1> We use the ProLong Antifade Kit (P7481) from Molecular probes. It comes as: 1. Powdered ProLong antifade reagent (Component A) 20 vials of powder. 2. ProLong mounting medium (Component B) in 2 bottles - 15 mL each. 1mL of component B should be mixed well in a vial of powder. This should be used in 24 hours. It works great. Michelle From joelleweaver <@t> hotmail.com Thu Mar 26 16:52:47 2009 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 26 16:52:53 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E801A8305F@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E801A8305F@EXCHANGECLUSTER.yumaregional.local> Message-ID: Well, I can't speak for everyone of course, but I know in the program that I am affiliate with that we stress, if not require, thinking beyond the manual methods. In fact, I really see an in depth understanding of basic manual histology methods as only a beginning point to how I want the future histologists to think and apply their technical knowledge.I encourage this at every opportunity myself, in every course. Crtical thinking skills, process thinking and the ability to see how our function fits into total laboratory and diagnostic patient services is stressed. I see it as imperative that this is incorporated into training in formal programs and within the lab. We cannot afford to not further this trend. I really don't see any other alternative really. If you look at newer instrumentation, it really is little more than a computer with software and application interfaces connected to the mechanics that perform the tasks of histology. Technology is really just another tool at our disposal to perform our jobs better. We in histology, are due to begin to merge and become cohesive with the totality of healthcare delivery and to begin to operate in such a parallel manner. I believe that the emerging histologist will be better armed with a broader educational background that provides this insight,due to more structured training program requirments. I for one, certainly hope that this is the "next wave" of evolution in our practice! Joelle Weaver HTL(ASCP) > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > Date: Thu, 26 Mar 2009 14:10:14 -0700 > From: JEllin@yumaregional.org > To: joelleweaver@hotmail.com > > I want to throw this notion out there as well,, how are we training our > techs to think? I would say that the majority of the histology programs > still teach to manual methodology rather than to think out of the box > and provide a total solution to the problem (with work flow and > technology). In my experience I see that people tend to take technology > and imitate their current manual process's rather than looking at > improving the current one. I like to hear what other people think on > this matter? It seems to me that there is a hunger for this technology > within the histology community but a lack of knowledge on how to > implement a viable solution to our current manual problems.. > > We are starting to see the coming of age again, an evolution within our > field were a technology similar to IHC, FISH, etc, will change the > course on how we tend to do things in the future. But we are not > educating our selves or our replacements, on how to handle these issues. > We cannot continue to solve a problem at the same level of thinking as > the previous solution. We need to begin to have a culture and > environmental shift in order for this technology to be adapted by the > industry. But people we are not looking at the downstream affect of > what our actions are, for example transcription, pathologist, send out > etc.. I am also very passionate about this.. > > The University of Washington has done some excellent work on this > solution from a histology level and as for Path view, I have heard good > things, but you all mention barcode, incorporation, and technology. But > what I have not heard is that it is the people that drive this to the > fore front. This would create a "Anatomic Histo/Pathology Improvement > System" were technology, methodology improvement, and people come > together to create an efficient way to handle our issues. > > Sorry for the long winded remark but I am also passionate about this > > > > > Jesus A Ellin HT/PA ASCP > > Department of Pathology/Histology > > Yuma Regional Medical Center > > 2400 South Ave A > > Yuma, AZ 85364 - 7170 > > ( Office: (928) 336-1743 > > ( Fax: (928) 336-7319 > > * Email: jellin@yumaregional.org > > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. _________________________________________________________________ Windows Live? SkyDrive: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009 From mike <@t> pathview.com Thu Mar 26 18:14:21 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Mar 26 18:16:03 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: References: <29BE166A2CF48D459853F8EC57CD37E801A8305F@EXCHANGECLUSTER.yumaregional.local> Message-ID: <012a01c9ae68$9fd862c0$df892840$@com> People are always at the forefront. Someone has to build that new tool, or come up with some new process or whatever. That's why before we do any installation of our software, we spend what probably amounts to 100 to 200 hours interviewing and watching each clerk, PA, histotech, secretary, cytotech, and pathologist and THEN we propose how we would install and tailor our software. By the way, at the end of that analysis, people are usually pretty tired of hearing me ask 'why do you do that', but guess what -- you are way, way more likely to get 'buy in' from the staff. That tech you spoke to at 3 a.m. remembers that some computer geek took the time and effort and asked them how they would do things better. ...but let me address a real world issue. I am not versed in the technologies of many aspects of the AP/Cytology department (you'll never hear me speak on subjects of which stainer is better for instance), but I do know a few things about work flow and computerization. I like to illustrate via example, so let's try this one: In the real world, a histotechnologist may have only worked in let's say 3 or 4different labs in their life, and perhaps only 1 or 2 different computer systems. With that background, how are they supposed to know what's possible or not possible to do with computer technology. Personally, I think it's the job of the LIS vendor to work TOGETHER with the histotechnologist and other department personnel to come up with better solutions. In this example, each side has knowledge and experience that needs to be conveyed to the other. When that communication occurs, magic happens. Barcodes are not the magic. It's how you use those barcodes in your work flow. It's always about the people. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, March 26, 2009 4:53 PM To: jellin@yumaregional.org; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Well, I can't speak for everyone of course, but I know in the program that I am affiliate with that we stress, if not require, thinking beyond the manual methods. In fact, I really see an in depth understanding of basic manual histology methods as only a beginning point to how I want the future histologists to think and apply their technical knowledge.I encourage this at every opportunity myself, in every course. Crtical thinking skills, process thinking and the ability to see how our function fits into total laboratory and diagnostic patient services is stressed. I see it as imperative that this is incorporated into training in formal programs and within the lab. We cannot afford to not further this trend. I really don't see any other alternative really. If you look at newer instrumentation, it really is little more than a computer with software and application interfaces connected to the mechanics that perform the tasks of histology. Technology is really just another tool at our disposal to perform our jobs better. We in histology, are due to begin to merge and become cohesive with the totality of healthcare delivery and to begin to operate in such a parallel manner. I believe that the emerging histologist will be better armed with a broader educational background that provides this insight,due to more structured training program requirments. I for one, certainly hope that this is the "next wave" of evolution in our practice! Joelle Weaver HTL(ASCP) > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > Date: Thu, 26 Mar 2009 14:10:14 -0700 > From: JEllin@yumaregional.org > To: joelleweaver@hotmail.com > > I want to throw this notion out there as well,, how are we training our > techs to think? I would say that the majority of the histology programs > still teach to manual methodology rather than to think out of the box > and provide a total solution to the problem (with work flow and > technology). In my experience I see that people tend to take technology > and imitate their current manual process's rather than looking at > improving the current one. I like to hear what other people think on > this matter? It seems to me that there is a hunger for this technology > within the histology community but a lack of knowledge on how to > implement a viable solution to our current manual problems.. > > We are starting to see the coming of age again, an evolution within our > field were a technology similar to IHC, FISH, etc, will change the > course on how we tend to do things in the future. But we are not > educating our selves or our replacements, on how to handle these issues. > We cannot continue to solve a problem at the same level of thinking as > the previous solution. We need to begin to have a culture and > environmental shift in order for this technology to be adapted by the > industry. But people we are not looking at the downstream affect of > what our actions are, for example transcription, pathologist, send out > etc.. I am also very passionate about this.. > > The University of Washington has done some excellent work on this > solution from a histology level and as for Path view, I have heard good > things, but you all mention barcode, incorporation, and technology. But > what I have not heard is that it is the people that drive this to the > fore front. This would create a "Anatomic Histo/Pathology Improvement > System" were technology, methodology improvement, and people come > together to create an efficient way to handle our issues. > > Sorry for the long winded remark but I am also passionate about this > > > > > Jesus A Ellin HT/PA ASCP > > Department of Pathology/Histology > > Yuma Regional Medical Center > > 2400 South Ave A > > Yuma, AZ 85364 - 7170 > > ( Office: (928) 336-1743 > > ( Fax: (928) 336-7319 > > * Email: jellin@yumaregional.org > > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. _________________________________________________________________ Windows LiveT SkyDrive: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009____ ___________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Thu Mar 26 20:37:47 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Mar 26 20:38:13 2009 Subject: [Histonet] Commercial Eosin Message-ID: Hi Vanessa, Yes! I have our eosin staining down to just 10 secs! I am considering just letting the slides hover over top of the eosin for a minute instead of actually dipping! LOL Very strong indeed. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Vanessa J. Phelan" 3/26/2009 3:23:49 PM >>> Hi everyone, Has anyone had the experience of massive overstaining with commercial Surgipath Alcohol-based Eosin? I manually stained with Surgipath Hx for 2mins, then bluer the eosin for 30secs and the whole tissue was completely pink! Thanks V _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From ooi.ting.huay <@t> nhc.com.sg Thu Mar 26 20:50:42 2009 From: ooi.ting.huay <@t> nhc.com.sg (ooi.ting.huay@nhc.com.sg) Date: Thu Mar 26 20:50:53 2009 Subject: [Histonet] Different between Richard Allan and Harris Hematoxylin Message-ID: Hi, I am interested to know what is the different between different brands of hematoxylin especially for Richard Allan and Harris. I am glad if there is any advice or suggestion on the choosing of hematoxylin. Welcome any suggestion of webpage that may tell the differences too. I am dealing with plastic resin section and doing a normal hematoxylin and eosin staining. Your advice are appreciated. Thank you very much! Regards, Ooi _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer From froyer <@t> bitstream.net Thu Mar 26 21:50:45 2009 From: froyer <@t> bitstream.net (froyer@bitstream.net) Date: Thu Mar 26 21:50:52 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: References: Message-ID: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> Dear Jennifer and any other Histonetters that are of like mind, It's not quite "Flaming Friday" yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has ?purchased all used equipment?. What vendor did you purchase from that did not provide operator?s manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let?s make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great R&D expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor?s expense for the shipping & handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you ?won?t hold your breath for that?. My Second Question: Are you crazy? >From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you ?will repay the favor, if at all possible?. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year?s supply of PH20 when it is released to the public... all at no charge, including shipping & handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford ?the demon vendor? Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of > this message to any person other than the intended recipient(s) is not > intended in any way to waive confidentiality or any applicable privilege. > Any review, retransmission, dissemination or other use of, or taking of > any action in reliance upon, this information by individuals or entities > other than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please > contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Fri Mar 27 04:47:48 2009 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Mar 27 04:47:57 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: <012a01c9ae68$9fd862c0$df892840$@com> References: <29BE166A2CF48D459853F8EC57CD37E801A8305F@EXCHANGECLUSTER.yumaregional.local> <012a01c9ae68$9fd862c0$df892840$@com> Message-ID: Yes, I do agree, that is why I call it a "tool" for people to use. I think that it is a stereotype to think that histologists are not experienced or knowledgable about computers. There are some histologists who have had a fairly good introduction to computer systems, how computers work, what they can and cannot do, software, applications, interfaces, databases, and have worked with 5 or more LIS systems, barcodes etc. Though admittedly, in my experience this is a rarity. Most of what I have learned about computers, I have gotten from formal classes, but I also have used this knowledge in other arenas, and wish I could use it more in my job. I am just not fortunate enough to have been given the opportunity to have much influence on the processes, or the computer systems. I think that many who have been promoted into management simply also accept this stereotype that histologists know only technical information, and so we are not consulted, though we do the work everyday.I wish that you could come to our lab and educate those who have been given this authority! I would love to have a "computer geek" come to our lab and inform us of what is available to help us to our jobs better. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: joelleweaver@hotmail.com; jellin@yumaregional.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > Date: Thu, 26 Mar 2009 18:14:21 -0500 > > People are always at the forefront. Someone has to build that new tool, or > come up with some new process or whatever. That's why before we do any > installation of our software, we spend what probably amounts to 100 to 200 > hours interviewing and watching each clerk, PA, histotech, secretary, > cytotech, and pathologist and THEN we propose how we would install and > tailor our software. By the way, at the end of that analysis, people are > usually pretty tired of hearing me ask 'why do you do that', but guess what > -- you are way, way more likely to get 'buy in' from the staff. That tech > you spoke to at 3 a.m. remembers that some computer geek took the time and > effort and asked them how they would do things better. > > > ...but let me address a real world issue. I am not versed in the > technologies of many aspects of the AP/Cytology department (you'll never > hear me speak on subjects of which stainer is better for instance), but I do > know a few things about work flow and computerization. I like to illustrate > via example, so let's try this one: > > In the real world, a histotechnologist may have only worked in let's say 3 > or 4different labs in their life, and perhaps only 1 or 2 different computer > systems. With that background, how are they supposed to know what's > possible or not possible to do with computer technology. Personally, I > think it's the job of the LIS vendor to work TOGETHER with the > histotechnologist and other department personnel to come up with better > solutions. In this example, each side has knowledge and experience that > needs to be conveyed to the other. When that communication occurs, magic > happens. Barcodes are not the magic. It's how you use those barcodes in > your work flow. > > > It's always about the people. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Thursday, March 26, 2009 4:53 PM > To: jellin@yumaregional.org; Histonet > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > > Well, I can't speak for everyone of course, but I know in the program that I > am affiliate with that we stress, if not require, thinking beyond the manual > methods. In fact, I really see an in depth understanding of basic manual > histology methods as only a beginning point to how I want the future > histologists to think and apply their technical knowledge.I encourage this > at every opportunity myself, in every course. Crtical thinking skills, > process thinking and the ability to see how our function fits into total > laboratory and diagnostic patient services is stressed. I see it as > imperative that this is incorporated into training in formal programs and > within the lab. We cannot afford to not further this trend. I really don't > see any other alternative really. > > If you look at newer instrumentation, it really is little more than a > computer with software and application interfaces connected to the mechanics > that perform the tasks of histology. Technology is really just another tool > at our disposal to perform our jobs better. We in histology, are due to > begin to merge and become cohesive with the totality of healthcare delivery > and to begin to operate in such a parallel manner. I believe that the > emerging histologist will be better armed with a broader educational > background that provides this insight,due to more structured training > program requirments. I for one, certainly hope that this is the "next wave" > of evolution in our practice! > > Joelle Weaver HTL(ASCP) > > > Subject: RE: [Histonet] Information Systems: Specimen Tracking & > MiddleWare > > Date: Thu, 26 Mar 2009 14:10:14 -0700 > > From: JEllin@yumaregional.org > > To: joelleweaver@hotmail.com > > > > I want to throw this notion out there as well,, how are we training our > > techs to think? I would say that the majority of the histology programs > > still teach to manual methodology rather than to think out of the box > > and provide a total solution to the problem (with work flow and > > technology). In my experience I see that people tend to take technology > > and imitate their current manual process's rather than looking at > > improving the current one. I like to hear what other people think on > > this matter? It seems to me that there is a hunger for this technology > > within the histology community but a lack of knowledge on how to > > implement a viable solution to our current manual problems.. > > > > We are starting to see the coming of age again, an evolution within our > > field were a technology similar to IHC, FISH, etc, will change the > > course on how we tend to do things in the future. But we are not > > educating our selves or our replacements, on how to handle these issues. > > We cannot continue to solve a problem at the same level of thinking as > > the previous solution. We need to begin to have a culture and > > environmental shift in order for this technology to be adapted by the > > industry. But people we are not looking at the downstream affect of > > what our actions are, for example transcription, pathologist, send out > > etc.. I am also very passionate about this.. > > > > The University of Washington has done some excellent work on this > > solution from a histology level and as for Path view, I have heard good > > things, but you all mention barcode, incorporation, and technology. But > > what I have not heard is that it is the people that drive this to the > > fore front. This would create a "Anatomic Histo/Pathology Improvement > > System" were technology, methodology improvement, and people come > > together to create an efficient way to handle our issues. > > > > Sorry for the long winded remark but I am also passionate about this > > > > > > > > > > Jesus A Ellin HT/PA ASCP > > > > Department of Pathology/Histology > > > > Yuma Regional Medical Center > > > > 2400 South Ave A > > > > Yuma, AZ 85364 - 7170 > > > > ( Office: (928) 336-1743 > > > > ( Fax: (928) 336-7319 > > > > * Email: jellin@yumaregional.org > > > > > > > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > _________________________________________________________________ > Windows LiveT SkyDrive: Get 25 GB of free online storage. > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009____ > ___________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ Windows Live? SkyDrive: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009 From histonet.nospam <@t> vneubert.com Fri Mar 27 04:56:59 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Mar 27 04:57:03 2009 Subject: [Histonet] Re-processing cartilage Message-ID: <7f1c5cb47bf57274ad1ce5d6bc12f1f8@vneubert.com> Hello Histonetters, I have processed some strips of cartilage (about 2cm long, 3mm of height, 2mm wide) with my standard protocol. I cannot section them. They just start to break away as soon as they hit the blade. I tried to section them really cold and also at RT, but that did not have any effect. I guess sth went wrong when processing (other kinds of tissue were fine). My protocol: Reagent Time [h] Spin (Microm ST120) NBF 1 1 70% Isopropanol 1 1 80% Isopropanol 1 1 95% Isopropanol 1 1 abs Isopropanol 1 1 abs Isopropanol 1 1 abs Isopropanol 1 1 Xylene 1 1 Xylene 1 1 Xylene 1 1 Paraffin I 1 1 Paraffin II 1 1 Maybe someone can help me out and give some hints. Thanks a lot! V. Neubert From ian.montgomery <@t> bio.gla.ac.uk Fri Mar 27 05:38:26 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Mar 27 05:40:46 2009 Subject: [Histonet] UK only. Message-ID: <13BF4CEEC41747268D17C008AC303BD0@IBLS.GLA.AC.UK> One of the departments in the University runs a class each year where they have the student's process, section and stain a batch of tissues. The microtome's they use have come from Noah's histology lab on the Ark. The mechanical workshop tries to keep them functional but they are really beyond economic repair. Obviously, re-equipping with new for a once in the year class is not an option and carrying microtome's around the campus is definitely not the favoured option. Does anyone know of a supplier of refurbished microtomes in the UK? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From mike <@t> pathview.com Fri Mar 27 07:08:05 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Mar 27 07:09:09 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: References: <29BE166A2CF48D459853F8EC57CD37E801A8305F@EXCHANGECLUSTER.yumaregional.local> <012a01c9ae68$9fd862c0$df892840$@com> Message-ID: <01b701c9aed4$b5bde2b0$2139a810$@com> Yea, I probably didn't communicate quite clearly enough, but I didn't want to elaborate too much as I suspect some people may be getting tired of hearing me talk. .but yes, invariably in every lab I run into, I find at least one person in each area who is more knowledgeable about computers than others and the good news, is that I suspect that trend will increase. Remember a lot of people in this area didn't grow up with computers. The newer generations are quite different. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Friday, March 27, 2009 4:48 AM To: mike@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Yes, I do agree, that is why I call it a "tool" for people to use. I think that it is a stereotype to think that histologists are not experienced or knowledgable about computers. There are some histologists who have had a fairly good introduction to computer systems, how computers work, what they can and cannot do, software, applications, interfaces, databases, and have worked with 5 or more LIS systems, barcodes etc. Though admittedly, in my experience this is a rarity. Most of what I have learned about computers, I have gotten from formal classes, but I also have used this knowledge in other arenas, and wish I could use it more in my job. I am just not fortunate enough to have been given the opportunity to have much influence on the processes, or the computer systems. I think that many who have been promoted into management simply also accept this stereotype that histologists know only technical information, and so we are not consulted, though we do the work everyday.I wish that you could come to our lab and educate those who have been given this authority! I would love to have a "computer geek" come to our lab and inform us of what is available to help us to our jobs better. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: joelleweaver@hotmail.com; jellin@yumaregional.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > Date: Thu, 26 Mar 2009 18:14:21 -0500 > > People are always at the forefront. Someone has to build that new tool, or > come up with some new process or whatever. That's why before we do any > installation of our software, we spend what probably amounts to 100 to 200 > hours interviewing and watching each clerk, PA, histotech, secretary, > cytotech, and pathologist and THEN we propose how we would install and > tailor our software. By the way, at the end of that analysis, people are > usually pretty tired of hearing me ask 'why do you do that', but guess what > -- you are way, way more likely to get 'buy in' from the staff. That tech > you spoke to at 3 a.m. remembers that some computer geek took the time and > effort and asked them how they would do things better. > > > ...but let me address a real world issue. I am not versed in the > technologies of many aspects of the AP/Cytology department (you'll never > hear me speak on subjects of which stainer is better for instance), but I do > know a few things about work flow and computerization. I like to illustrate > via example, so let's try this one: > > In the real world, a histotechnologist may have only worked in let's say 3 > or 4different labs in their life, and perhaps only 1 or 2 different computer > systems. With that background, how are they supposed to know what's > possible or not possible to do with computer technology. Personally, I > think it's the job of the LIS vendor to work TOGETHER with the > histotechnologist and other department personnel to come up with better > solutions. In this example, each side has knowledge and experience that > needs to be conveyed to the other. When that communication occurs, magic > happens. Barcodes are not the magic. It's how you use those barcodes in > your work flow. > > > It's always about the people. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Thursday, March 26, 2009 4:53 PM > To: jellin@yumaregional.org; Histonet > Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare > > > Well, I can't speak for everyone of course, but I know in the program that I > am affiliate with that we stress, if not require, thinking beyond the manual > methods. In fact, I really see an in depth understanding of basic manual > histology methods as only a beginning point to how I want the future > histologists to think and apply their technical knowledge.I encourage this > at every opportunity myself, in every course. Crtical thinking skills, > process thinking and the ability to see how our function fits into total > laboratory and diagnostic patient services is stressed. I see it as > imperative that this is incorporated into training in formal programs and > within the lab. We cannot afford to not further this trend. I really don't > see any other alternative really. > > If you look at newer instrumentation, it really is little more than a > computer with software and application interfaces connected to the mechanics > that perform the tasks of histology. Technology is really just another tool > at our disposal to perform our jobs better. We in histology, are due to > begin to merge and become cohesive with the totality of healthcare delivery > and to begin to operate in such a parallel manner. I believe that the > emerging histologist will be better armed with a broader educational > background that provides this insight,due to more structured training > program requirments. I for one, certainly hope that this is the "next wave" > of evolution in our practice! > > Joelle Weaver HTL(ASCP) > > > Subject: RE: [Histonet] Information Systems: Specimen Tracking & > MiddleWare > > Date: Thu, 26 Mar 2009 14:10:14 -0700 > > From: JEllin@yumaregional.org > > To: joelleweaver@hotmail.com > > > > I want to throw this notion out there as well,, how are we training our > > techs to think? I would say that the majority of the histology programs > > still teach to manual methodology rather than to think out of the box > > and provide a total solution to the problem (with work flow and > > technology). In my experience I see that people tend to take technology > > and imitate their current manual process's rather than looking at > > improving the current one. I like to hear what other people think on > > this matter? It seems to me that there is a hunger for this technology > > within the histology community but a lack of knowledge on how to > > implement a viable solution to our current manual problems.. > > > > We are starting to see the coming of age again, an evolution within our > > field were a technology similar to IHC, FISH, etc, will change the > > course on how we tend to do things in the future. But we are not > > educating our selves or our replacements, on how to handle these issues. > > We cannot continue to solve a problem at the same level of thinking as > > the previous solution. We need to begin to have a culture and > > environmental shift in order for this technology to be adapted by the > > industry. But people we are not looking at the downstream affect of > > what our actions are, for example transcription, pathologist, send out > > etc.. I am also very passionate about this.. > > > > The University of Washington has done some excellent work on this > > solution from a histology level and as for Path view, I have heard good > > things, but you all mention barcode, incorporation, and technology. But > > what I have not heard is that it is the people that drive this to the > > fore front. This would create a "Anatomic Histo/Pathology Improvement > > System" were technology, methodology improvement, and people come > > together to create an efficient way to handle our issues. > > > > Sorry for the long winded remark but I am also passionate about this > > > > > > > > > > Jesus A Ellin HT/PA ASCP > > > > Department of Pathology/Histology > > > > Yuma Regional Medical Center > > > > 2400 South Ave A > > > > Yuma, AZ 85364 - 7170 > > > > ( Office: (928) 336-1743 > > > > ( Fax: (928) 336-7319 > > > > * Email: jellin@yumaregional.org > > > > > > > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > _________________________________________________________________ > Windows LiveT SkyDrive: Get 25 GB of free online storage. > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009____ > ___________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _____ Windows LiveT SkyDrive: Get 25 GB of free online storage. Check it out. From JEllin <@t> yumaregional.org Fri Mar 27 07:37:25 2009 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Mar 27 07:37:29 2009 Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare In-Reply-To: Message-ID: <29BE166A2CF48D459853F8EC57CD37E801A8306A@EXCHANGECLUSTER.yumaregional.local> I do agree that computers are tools that are indeed an asset to anatomic path laboratories. Michael I applaud you for your efforts in getting the staff and engaging them. This is the basis of my entire theory, in order to create efficiencies within histology that there are 3 distinct area of the process Histology, Transcription, and Pathologist. Unlike the clinical laboratory we are not a straight test result type of methodology, rather a fair straight forward process that has inter-connected components. Those components have for so long relied o the fact of internal checks and balances, but with the explosion that has happened within AP in the last 10-15 years we are seeing those checks and balances begin to have cracks and stress points. I would applaud anyone that takes advance courses in anything, but I would caution an IT person looking at Anatomic Pathology that does not have the clinical background that is necessary to see the cracks and stress points. I use PowerPath as my LIS and as the University of Washington our facility has made strides in stream lining and innovation with our LIS,, but I am open to help anyone that is looking to get information on this subject. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, March 27, 2009 2:48 AM To: mike@pathview.com; Histonet Subject: RE: [Histonet] Information Systems: Specimen Tracking & MiddleWare Yes, I do agree, that is why I call it a "tool" for people to use. I think that it is a stereotype to think that histologists are not experienced or knowledgable about computers. There are some histologists who have had a fairly good introduction to computer systems, how computers work, what they can and cannot do, software, applications, interfaces, databases, and have worked with 5 or more LIS systems, barcodes etc. Though admittedly, in my experience this is a rarity. Most of what I have learned about computers, I have gotten from formal classes, but I also have used this knowledge in other arenas, and wish I could use it more in my job. I am just not fortunate enough to have been given the opportunity to have much influence on the processes, or the computer systems. I think that many who have been promoted into management simply also accept this stereotype that histologists know only technical information, and so we are not consulted, though we do the work everyday.I wish that you could come to our lab and educate those who have been given this authority! I would love to have a "computer geek" come to our lab and inform us of what is available to help us to our jobs better. Joelle Weaver HTL (ASCP) > From: mike@pathview.com > To: joelleweaver@hotmail.com; jellin@yumaregional.org; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Information Systems: Specimen Tracking & > MiddleWare > Date: Thu, 26 Mar 2009 18:14:21 -0500 > > People are always at the forefront. Someone has to build that new > tool, or come up with some new process or whatever. That's why before > we do any installation of our software, we spend what probably amounts > to 100 to 200 hours interviewing and watching each clerk, PA, > histotech, secretary, cytotech, and pathologist and THEN we propose > how we would install and tailor our software. By the way, at the end > of that analysis, people are usually pretty tired of hearing me ask > 'why do you do that', but guess what > -- you are way, way more likely to get 'buy in' from the staff. That > tech you spoke to at 3 a.m. remembers that some computer geek took the > time and effort and asked them how they would do things better. > > > ...but let me address a real world issue. I am not versed in the > technologies of many aspects of the AP/Cytology department (you'll > never hear me speak on subjects of which stainer is better for > instance), but I do know a few things about work flow and > computerization. I like to illustrate via example, so let's try this one: > > In the real world, a histotechnologist may have only worked in let's > say 3 or 4different labs in their life, and perhaps only 1 or 2 > different computer systems. With that background, how are they > supposed to know what's possible or not possible to do with computer > technology. Personally, I think it's the job of the LIS vendor to work > TOGETHER with the histotechnologist and other department personnel to > come up with better solutions. In this example, each side has > knowledge and experience that needs to be conveyed to the other. When > that communication occurs, magic happens. Barcodes are not the magic. > It's how you use those barcodes in your work flow. > > > It's always about the people. > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: > 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Thursday, March 26, 2009 4:53 PM > To: jellin@yumaregional.org; Histonet > Subject: RE: [Histonet] Information Systems: Specimen Tracking & > MiddleWare > > > Well, I can't speak for everyone of course, but I know in the program > that I am affiliate with that we stress, if not require, thinking > beyond the manual methods. In fact, I really see an in depth > understanding of basic manual histology methods as only a beginning > point to how I want the future histologists to think and apply their > technical knowledge.I encourage this at every opportunity myself, in > every course. Crtical thinking skills, process thinking and the > ability to see how our function fits into total laboratory and > diagnostic patient services is stressed. I see it as imperative that > this is incorporated into training in formal programs and within the > lab. We cannot afford to not further this trend. I really don't see any other alternative really. > > If you look at newer instrumentation, it really is little more than a > computer with software and application interfaces connected to the > mechanics that perform the tasks of histology. Technology is really > just another tool at our disposal to perform our jobs better. We in > histology, are due to begin to merge and become cohesive with the > totality of healthcare delivery and to begin to operate in such a > parallel manner. I believe that the emerging histologist will be > better armed with a broader educational background that provides this > insight,due to more structured training program requirments. I for one, certainly hope that this is the "next wave" > of evolution in our practice! > > Joelle Weaver HTL(ASCP) > > > Subject: RE: [Histonet] Information Systems: Specimen Tracking & > MiddleWare > > Date: Thu, 26 Mar 2009 14:10:14 -0700 > > From: JEllin@yumaregional.org > > To: joelleweaver@hotmail.com > > > > I want to throw this notion out there as well,, how are we training > > our techs to think? I would say that the majority of the histology > > programs still teach to manual methodology rather than to think out > > of the box and provide a total solution to the problem (with work > > flow and technology). In my experience I see that people tend to > > take technology and imitate their current manual process's rather > > than looking at improving the current one. I like to hear what other > > people think on this matter? It seems to me that there is a hunger > > for this technology within the histology community but a lack of > > knowledge on how to implement a viable solution to our current manual problems.. > > > > We are starting to see the coming of age again, an evolution within > > our field were a technology similar to IHC, FISH, etc, will change > > the course on how we tend to do things in the future. But we are not > > educating our selves or our replacements, on how to handle these issues. > > We cannot continue to solve a problem at the same level of thinking > > as the previous solution. We need to begin to have a culture and > > environmental shift in order for this technology to be adapted by > > the industry. But people we are not looking at the downstream affect > > of what our actions are, for example transcription, pathologist, > > send out etc.. I am also very passionate about this.. > > > > The University of Washington has done some excellent work on this > > solution from a histology level and as for Path view, I have heard > > good things, but you all mention barcode, incorporation, and > > technology. But what I have not heard is that it is the people that > > drive this to the fore front. This would create a "Anatomic > > Histo/Pathology Improvement System" were technology, methodology > > improvement, and people come together to create an efficient way to handle our issues. > > > > Sorry for the long winded remark but I am also passionate about this > > > > > > > > > > Jesus A Ellin HT/PA ASCP > > > > Department of Pathology/Histology > > > > Yuma Regional Medical Center > > > > 2400 South Ave A > > > > Yuma, AZ 85364 - 7170 > > > > ( Office: (928) 336-1743 > > > > ( Fax: (928) 336-7319 > > > > * Email: jellin@yumaregional.org > > > > > > > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged or > > exempt from disclosure under applicable law. If you are not the > > intended recipient(s), you are notified that the dissemination, > > distribution, or copying of this message is strictly prohibited. If > > you receive this message in error, or are not the named > > recipient(s), please notify the sender at either the e-mail, fax, > > address, or telephone number listed above and delete this e-mail > > from your computer. > > Thank You. > > _________________________________________________________________ > Windows LiveT SkyDrive: Get 25 GB of free online storage. > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_0320 > 09____ ___________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ Windows Live(tm) SkyDrive: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From froyer <@t> bitstream.net Fri Mar 27 08:04:13 2009 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Mar 27 08:04:20 2009 Subject: [Histonet] UK only. In-Reply-To: <13BF4CEEC41747268D17C008AC303BD0@IBLS.GLA.AC.UK> References: <13BF4CEEC41747268D17C008AC303BD0@IBLS.GLA.AC.UK> Message-ID: <2BC11F0C95F1495AB4EC88A904237C92@Ford> Ian, Contact Colin Shandley of Colco Scientific Enterprises Ltd., 6 Peatmore Close, Woking, Surrey. Phone: 1 932 349 141. He brokers used lab equipment and while his main area is in hematology and chemistry lab equipment, he occasionally comes across histology equipment. He may be able to locate microtomes for you. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Friday, March 27, 2009 5:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] UK only. One of the departments in the University runs a class each year where they have the student's process, section and stain a batch of tissues. The microtome's they use have come from Noah's histology lab on the Ark. The mechanical workshop tries to keep them functional but they are really beyond economic repair. Obviously, re-equipping with new for a once in the year class is not an option and carrying microtome's around the campus is definitely not the favoured option. Does anyone know of a supplier of refurbished microtomes in the UK? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbott.com Fri Mar 27 08:43:48 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Fri Mar 27 08:43:56 2009 Subject: [Histonet] Cortical bone Image analysis fluorescent calcine measurement Message-ID: Hi All, Question for anyone working with image analysis on Bone.. My 1st goal: Evaluate and purchase image analysis software to measure bone formation rate (BFR) and mineral apposition rate (MAR). I am trying to measure the area between two fluorescent (calcine) labels of cortical bone by image analysis. This is standard for bone histomorphometrics in all the literature I see but as always there is a few details that are missing. Papers don't say what object they use, but for my mouse and rat tibia samples, 10x won't get the whole sample in one frame? I have notes from someone who has done this and they trace the perimeters to get the data (with osteometric software). If I do tracing at 4x I wonder about the accuracy. Osteometrics is the most common bone morphometric system out there but for my cortical bone it seems a little overkill. I would like to use Zeiss axiovision software anyone using this? I think people are using 10-20x and trace the perimeter of the bone but I am wondering if with certain software you can move along the perimeter of the bone without stopping the data collection or do you have to collect multiple data sets and sum the total to get the perimeter. My 2nd goal: Evaluate confocal microscopy imaging of bone for reducing in time spent grind samples. This method was based on a short communication in Journal of Histochemical "rapid method for the assessment of bone architecture by confocal microscopy." Right now I am trying to determine what imaging modality I am going to use for these measurements. My pathologists wants me to do large rough cuts (625-1000um) from my isomet saw and see if I can measure these labels under confocal (ie eliminating grinding) , has anyone tried this? I can tell you that I can image these samples without a coverslip but the samples is uneven due no grinding or placement of sample on the slide. The theory is that I can image these samples at 10 or 20x and get an image some microns into the sample thus eliminating the uneven (rough) upper surface. In practice I can get some areas to look great but I can't focus all the way around the bone I lose the image focus possible due to a thicker side of bone,, so I think this idea is not practical. Has anyone tried this? I think going forward I will grinding samples to about 20um and mount the samples in water based media and coverslip. If you do this type of work please contact me I'd love to talk about this... As always the powers to be are demanding validation studies of this method be done yesterday and I haven't even decided on software. Not to mention that I am new to bone work and I haven't even ground my first bone sample. Anyway I'll get there, thanks for any help "Happy Friday". Jamie _______________________________ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From ratliffjack <@t> hotmail.com Fri Mar 27 09:04:55 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Mar 27 09:05:10 2009 Subject: [Histonet] Cortical bone Image analysis fluorescent calcine measurement In-Reply-To: References: Message-ID: Try contacting Nathanael Reveal @ BIOQUANT Image Analysis Corp. They are also having an image analysis workshop in Nashville, TN on April 21-23. It will cover all aspects of typical bone image analysis and even feature a one day session on specimen preparation using MMA techniques. Jack Ratliff On Mar 27, 2009, at 8:43 AM, Jamie E Erickson wrote: > Hi All, > Question for anyone working with image analysis on Bone.. > > My 1st goal: Evaluate and purchase image analysis software to > measure bone > formation rate (BFR) and mineral apposition rate (MAR). > > I am trying to measure the area between two fluorescent > (calcine) > labels of cortical bone by image analysis. > This is standard for bone histomorphometrics in all the literature I > see > but as always there is a few details that are missing. > Papers don't say what object they use, but for my mouse and rat > tibia samples, 10x won't get the whole sample in one frame? I have > notes > from someone who has done this and they trace the perimeters to get > the > data (with osteometric software). If I do tracing at 4x I wonder > about > the accuracy. Osteometrics is the most common bone morphometric > system > out there but for my cortical bone it seems a little overkill. I would > like to use Zeiss axiovision software anyone using this? I think > people > are using 10-20x and trace the perimeter of the bone but I am > wondering if > with certain software you can move along the perimeter of the bone > without > stopping the data collection or do you have to collect multiple data > sets > and sum the total to get the perimeter. > > My 2nd goal: Evaluate confocal microscopy imaging of bone for > reducing in > time spent grind samples. > > This method was based on a short communication in Journal of > Histochemical "rapid method for the assessment of bone architecture by > confocal microscopy." > Right now I am trying to determine what imaging modality I am going > to use > for these measurements. My pathologists wants me to do large rough > cuts > (625-1000um) from my isomet saw and see if I can measure these labels > under confocal (ie eliminating grinding) , has anyone tried this? > I can tell you that I can image these samples without a > coverslip > but the samples is uneven due no grinding or placement of sample on > the > slide. The theory is that I can image these samples at 10 or 20x and > get > an image some microns into the sample thus eliminating the uneven > (rough) > upper surface. > In practice I can get some areas to look great but I can't focus > all the > way around the bone I lose the image focus possible due to a thicker > side > of bone,, so I think this idea is not practical. Has anyone tried > this? > > I think going forward I will grinding samples to about 20um and > mount the > samples in water based media and coverslip. > > If you do this type of work please contact me I'd love to talk about > this... As always the powers to be are demanding validation studies > of > this method be done yesterday and I haven't even decided on > software. Not > to mention that I am new to bone work and I haven't even ground my > first > bone sample. > > Anyway I'll get there, thanks for any help "Happy Friday". > > Jamie > > _______________________________ > Jamie Erickson > Sr. Research Associate II M.S. HTL (ASCP) > Department: DSMP > Abbott Bioresearch Center > 100 Research Drive > Worcester, MA 01605-4341 > 508-688-3134 > FAX: 508-793-4895 > e-mail: jamie.erickson@abbott.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From NMargaryan <@t> childrensmemorial.org Fri Mar 27 09:23:12 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Mar 27 09:23:38 2009 Subject: [Histonet] Re: Different between Richard Allan and Harris & which one is better for IHC Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601E6D5A3@CMHEXC01EVS.childrensmemorial.org> Hi all, I am adding my question to the below one. Which is better to use for IHC: Mayer's or just hematoxylin? Thanks in advance, Naira Message: 15 Date: Fri, 27 Mar 2009 09:50:42 +0800 From: ooi.ting.huay@nhc.com.sg Subject: [Histonet] Different between Richard Allan and Harris Hematoxylin Hi, I am interested to know what is the different between different brands of hematoxylin especially for Richard Allan and Harris. I am glad if there is any advice or suggestion on the choosing of hematoxylin. Welcome any suggestion of webpage that may tell the differences too. I am dealing with plastic resin section and doing a normal hematoxylin and eosin staining. Your advice is appreciated. Thank you very much! Regards, Ooi From SwainFrancesL <@t> uams.edu Fri Mar 27 09:44:54 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Mar 27 09:45:45 2009 Subject: [Histonet] Re: Different between Richard Allan and Harris & which one is better for IHC In-Reply-To: <6A2230BAC92E3B4084DAE06869B89FB601E6D5A3@CMHEXC01EVS.childrensmemorial.org> References: <6A2230BAC92E3B4084DAE06869B89FB601E6D5A3@CMHEXC01EVS.childrensmemorial.org> Message-ID: I use Mayer's Hematoxlyin for 30-45 secs. Wash for 2 minutes, blue in Ammonia Water, wash for 5 minutes, dehydrate clear and mount if it is DAB. If it is AEC I do the same except I rinse in distilled water before mounting with aqueous mount. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 27, 2009 9:23 AM To: ooi.ting.huay@nhc.com.sg; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Different between Richard Allan and Harris & which one is better for IHC Hi all, I am adding my question to the below one. Which is better to use for IHC: Mayer's or just hematoxylin? Thanks in advance, Naira Message: 15 Date: Fri, 27 Mar 2009 09:50:42 +0800 From: ooi.ting.huay@nhc.com.sg Subject: [Histonet] Different between Richard Allan and Harris Hematoxylin Hi, I am interested to know what is the different between different brands of hematoxylin especially for Richard Allan and Harris. I am glad if there is any advice or suggestion on the choosing of hematoxylin. Welcome any suggestion of webpage that may tell the differences too. I am dealing with plastic resin section and doing a normal hematoxylin and eosin staining. Your advice is appreciated. Thank you very much! Regards, Ooi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From barbara.verstraeten <@t> ugent.be Fri Mar 27 09:49:16 2009 From: barbara.verstraeten <@t> ugent.be (barbara.verstraeten@ugent.be) Date: Fri Mar 27 09:49:23 2009 Subject: [Histonet] Agar removing for fluorescent stainings Message-ID: <20090327154916.ihhluwyiowocwo08@webmail.ugent.be> Hey everybody! Today I tried to do a fluorescent staining on cryosectionned tissue embedded in agar. I didn't see a problem in the presence of the agar untill I analyzed the slides under the microscope... The agar had absorbed my fluorescent dy!! Does anyone know who to remove the agar where the tissue is embedded in to make it possible to do cryosections? I tried with 37?C PBS to 'melt' it and rinse it away but kept sticking on the slide.. Any advice? Thanks alot for your help! Greetings, Barbara Verstraeten, Drs. Vertebrate Morpholgy and Developmental Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 B-9000 Ghent Belgium tel: ++32/(0)9 264 52 31 fax: ++32/(0)9 264 53 44 From jnocito <@t> satx.rr.com Fri Mar 27 10:52:53 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Mar 27 10:53:00 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> Message-ID: <63A3C28579D0430993F87A8072ACEC53@JoePC> OUCH JTT ----- Original Message ----- From: To: "Jennifer Anderson" Cc: "Histonet" Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite "Flaming Friday" yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has "purchased all used equipment". What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great R&D expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping & handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you "won't hold your breath for that". My Second Question: Are you crazy? >From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you "will repay the favor, if at all possible". I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping & handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford "the demon vendor" Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of > this message to any person other than the intended recipient(s) is not > intended in any way to waive confidentiality or any applicable privilege. > Any review, retransmission, dissemination or other use of, or taking of > any action in reliance upon, this information by individuals or entities > other than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please > contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 <@t> msn.com Fri Mar 27 10:58:17 2009 From: steve8438 <@t> msn.com (Steven MELLO) Date: Fri Mar 27 10:58:22 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <63A3C28579D0430993F87A8072ACEC53@JoePC> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> <63A3C28579D0430993F87A8072ACEC53@JoePC> Message-ID: The response below is completely and utterly disrespectful and downright rude Steven Mello,HT(ASCP) Anatomical Pathology Manager > From: jnocito@satx.rr.com > To: froyer@bitstream.net; janderson@halozyme.com > Date: Fri, 27 Mar 2009 10:52:53 -0500 > Subject: Re: [Histonet] Please help! In dire need of user manuals > CC: histonet@lists.utsouthwestern.edu > > OUCH > > JTT > ----- Original Message ----- > From: > To: "Jennifer Anderson" > Cc: "Histonet" > Sent: Thursday, March 26, 2009 9:50 PM > Subject: Re: [Histonet] Please help! In dire need of user manuals > > > Dear Jennifer and any other Histonetters that are of like mind, > > It's not quite "Flaming Friday" yet, but it will be in just a few hours, > so I'll go ahead a little early with what I hope is a reply that may > enlighten, be consultative, and hopefully not too offensive to those that > may be sensitive to such replies. Here it goes... > > My First Question: You state that your lab has "purchased all used > equipment". What vendor did you purchase from that did not provide > operator's manuals with the equipment? This is unheard of amongst us > reputable used/refurbished equipment dealers so if I were you, I would not > recommend this vendor to others. > > Now let's make sure that I have the facts straight... You have posted a > request that is seeking charity from members of this list that also > includes vendors. In addition, not only are you asking for free copies of > publications that have been copyrighted and are the proprietary > information of the manufacturers that, at great R&D expenditure developed > them, but you are asking that the donor(s) mail hard copies (if available) > to you ...at the donor's expense for the shipping & handling. Should > hard-copies not be available, you request the donor(s) to provide these > lengthy documents in digital PDF format... again with a substantial > expense to the donor(s) of converting these publications into digital > form. Surprisingly, you have made this burdensome and expensive request > without any offer to reimburse the charitable donor(s) for their time and > expense to do all of this work for you. In your final statement you then > wistfully hope that some benevolent vendor should raise their hand and > offer you a (free) copy and then insult that same vendor buy stating that > you "won't hold your breath for that". > > My Second Question: Are you crazy? > > >From your signature address; it appears that you are employed by a company > called Halozyme Therapeutics. A quick web search informs us that Halozyme > is a for-profit California company that had revenues in the tens of > millions in 2008. It was also exciting to learn that Halozyme is in Phase > 2 clinical trials of their innovative insulin-PH20 with individuals that > suffer from Type 1 diabetes. Very impressive. > > Here is some good news for you. I happen to have ALL the operator manuals > for the equipment that you are requesting. I will copy them for you and > send them to you at my expense under one condition. You state that if > someone can do this for you that you "will repay the favor, if at all > possible". I have a family member that is a Type 1 insulin-dependent > diabetic. All I ask in return is that you arrange with your marketing > department that they send me a one-year's supply of PH20 when it is > released to the public... all at no charge, including shipping & handling > of course. > > My Third Question: Do we have a deal? > > I look forward to your reply, > > ~ Ford > > Ford "the demon vendor" Royer, MT(ASCP) > Histology Product Manager > Minnesota Medical, Inc. (a for-profit corporation just like yours) > Golden Valley, MN > > > > Hello Everyone. > > > > > > > > I our lab we've purchased all used equipment. None of these instruments > > came with a user's manual. I am in need of a manual (hard-copy or PDF) > > for the following: > > > > > > > > Sakura Tissue-Tek VIP 3000 > > > > Leica Jung Histo Embedder > > > > Microm HM335E Microtome > > > > > > > > I do realize that requesting a copy of these is a lot to ask of someone. > > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > > if at all possible. I'm hoping that a vendor may raise their hand and > > offer a copy? I won't hold my breath for that... > > > > > > > > Thanks everyone. > > > > > > > > Jennifer M. Anderson, Scientist > > > > Halozyme Therapeutics, Inc. > > > > 11404 Sorrento Valley Road > > > > San Diego, CA 92121 > > > > 858-704-8333 > > > > janderson@halozyme.com > > > > > > > > > > > > > > The information transmitted in this email is confidential and is intended > > only for the person(s) or entity to which it is addressed. Delivery of > > this message to any person other than the intended recipient(s) is not > > intended in any way to waive confidentiality or any applicable privilege. > > Any review, retransmission, dissemination or other use of, or taking of > > any action in reliance upon, this information by individuals or entities > > other than the intended recipient is prohibited by Halozyme and may be in > > violation of applicable laws. If you received this in error, please > > contact the sender and delete/destroy this email. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Mar 27 11:01:24 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Mar 27 11:01:39 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <63A3C28579D0430993F87A8072ACEC53@JoePC> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> <63A3C28579D0430993F87A8072ACEC53@JoePC> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0477@LTA3VS011.ees.hhs.gov> Double ouch! Somebody has their panties in a bunch today! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, March 27, 2009 11:53 AM To: froyer@bitstream.net; Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals OUCH JTT ----- Original Message ----- From: To: "Jennifer Anderson" Cc: "Histonet" Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite "Flaming Friday" yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has "purchased all used equipment". What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great R&D expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping & handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you "won't hold your breath for that". My Second Question: Are you crazy? >From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you "will repay the favor, if at all possible". I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping & handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford "the demon vendor" Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of > this message to any person other than the intended recipient(s) is not > intended in any way to waive confidentiality or any applicable privilege. > Any review, retransmission, dissemination or other use of, or taking of > any action in reliance upon, this information by individuals or entities > other than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please > contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Fri Mar 27 11:17:33 2009 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Mar 27 11:17:38 2009 Subject: [Histonet] tissues and static Message-ID: has anyone had any trouble when embedding little specimens they float to the sides of the molds like they are charged. it happens sometimes with our little core bxs. thanks, everyone have a good weekend! anita dudley providence hosp mobile alabama _________________________________________________________________ Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A From jmcgough <@t> clinlab.com Fri Mar 27 11:27:22 2009 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Fri Mar 27 11:27:29 2009 Subject: [Histonet] Staining vs. paraffin wax problems In-Reply-To: Message-ID: We are experiencing difficulties with our H&E staining and other Special Stains. After the slides are stained the edges of the tissue look fragmented/chattered. Our pathologists also thought the sections looked like they might be too thick but we checked all of our microtomes for thickness settings and all are set at 4 micrometers. There seems to be several blotches of light staining throughout the tissue. This seems to be only on our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene. Is this a deparaffinization, processing, or a staining issue? Any help is appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From billodonnell <@t> catholichealth.net Fri Mar 27 12:04:39 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Mar 27 12:04:54 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0477@LTA3VS011.ees.hhs.gov> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com><63A3C28579D0430993F87A8072ACEC53@JoePC> <9A16CB5D55FC1648ADF11B63E72A1BE1BF0477@LTA3VS011.ees.hhs.gov> Message-ID: "The secret of life is honesty and fair dealing. If you can fake that, you've got it made." - Groucho Marx, Double-dog ouch. Nuff said, I'm backing out now, William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, March 27, 2009 11:01 AM To: Joe Nocito; froyer@bitstream.net; Jennifer Anderson Cc: Histonet Subject: RE: [Histonet] Please help! In dire need of user manuals Double ouch! Somebody has their panties in a bunch today! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, March 27, 2009 11:53 AM To: froyer@bitstream.net; Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals OUCH JTT ----- Original Message ----- From: To: "Jennifer Anderson" Cc: "Histonet" Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite "Flaming Friday" yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has "purchased all used equipment". What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great R&D expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping & handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you "won't hold your breath for that". My Second Question: Are you crazy? >From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you "will repay the favor, if at all possible". I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping & handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford "the demon vendor" Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of > this message to any person other than the intended recipient(s) is not > intended in any way to waive confidentiality or any applicable privilege. > Any review, retransmission, dissemination or other use of, or taking of > any action in reliance upon, this information by individuals or entities > other than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please > contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Fri Mar 27 12:16:59 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Mar 27 12:24:17 2009 Subject: [Histonet] plastic slides for sale Message-ID: <7367C990B7D24A338652C861A705CC38@shop1e2e996aa5> For those who prepare specimens for ground histology we have plastic slides available. Since I am closing my lab we will no longer be selling custom plastic slides. We are closing our stock at half price. 2" x 3" x .030" clear 800 slides 2" x 3" x .060" clear 64 slides 2" x 3" x .060" white 769 slides 2" x 2" x .060" clear 88 slides 2" x 2" x .060" white 112 slides higher quality plastic slides 2" x 3" x .030" clear 346 slides Cathy Mayton Wasatch Histo Consultants, Inc. From Doug.Showers <@t> propath.com Fri Mar 27 12:25:57 2009 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Fri Mar 27 12:27:36 2009 Subject: [Histonet] Staining vs. paraffin wax problems In-Reply-To: References: Message-ID: <82C7248978CB50469FD6BA68EBBEFE67EB47C4@exchange.propathlab.com> I would suggest you send your recycled xylene for testing; the manufacturer of your recycler should be able to recommend several. I suspect water in the xylene (I have seen it cause this issue on the processor) or inadequate drying of the slides before deparaffinization. Any water droplets left on the slide will not be removed by the xylene on the stainer leaving tiny round areas in the tissue that still have paraffin in them. The hematoxylin and eosin will not fully stain these areas, but the xylene at the end of the H&E stain will remove this residual wax. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 214-237-1680 214-422-3083 Mobile 214-237-1706 FAX To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough Sent: Friday, March 27, 2009 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining vs. paraffin wax problems We are experiencing difficulties with our H&E staining and other Special Stains. After the slides are stained the edges of the tissue look fragmented/chattered. Our pathologists also thought the sections looked like they might be too thick but we checked all of our microtomes for thickness settings and all are set at 4 micrometers. There seems to be several blotches of light staining throughout the tissue. This seems to be only on our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene. Is this a deparaffinization, processing, or a staining issue? Any help is appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Paul <@t> Firnschild.com Fri Mar 27 12:32:12 2009 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Fri Mar 27 12:32:18 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <63A3C28579D0430993F87A8072ACEC53@JoePC> Message-ID: <000001c9af01$f8581500$6402a8c0@qa44c0f386def9> Jennifer: I will send you the VIP manual. I'm in service and sell some used equipment - I need all the customers I can get so I'm ready to help all I can (even though you are in CA and I'm in GA.) Paul Firnschild QA Support Services Inc. 404.291.3715 Paul@Firnschild.com -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, March 27, 2009 11:53 AM To: froyer@bitstream.net; Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals OUCH JTT ----- Original Message ----- From: To: "Jennifer Anderson" Cc: "Histonet" Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite "Flaming Friday" yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has "purchased all used equipment". What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great R&D expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping & handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you "won't hold your breath for that". My Second Question: Are you crazy? >From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you "will repay the favor, if at all possible". I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping & handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford "the demon vendor" Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of > this message to any person other than the intended recipient(s) is not > intended in any way to waive confidentiality or any applicable privilege. > Any review, retransmission, dissemination or other use of, or taking of > any action in reliance upon, this information by individuals or entities > other than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please > contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrobertson <@t> pathologysciences.com Fri Mar 27 12:48:14 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Fri Mar 27 12:48:19 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> Message-ID: <518CD6920AA7154193CBE5977CD88073177F23@psmgsrv2.PSMG.local> Flaming? This is total burn out! Very inappropriate. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of froyer@bitstream.net Sent: Thursday, March 26, 2009 7:51 PM To: Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite "Flaming Friday" yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has "purchased all used equipment". What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great R&D expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping & handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you "won't hold your breath for that". My Second Question: Are you crazy? >From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you "will repay the favor, if at all possible". I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping & handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford "the demon vendor" Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN > Hello Everyone. > > > > I our lab we've purchased all used equipment. None of these instruments > came with a user's manual. I am in need of a manual (hard-copy or PDF) > for the following: > > > > Sakura Tissue-Tek VIP 3000 > > Leica Jung Histo Embedder > > Microm HM335E Microtome > > > > I do realize that requesting a copy of these is a lot to ask of someone. > It takes a lot of time to copy a 50-page manual. I'll repay the favor, > if at all possible. I'm hoping that a vendor may raise their hand and > offer a copy? I won't hold my breath for that... > > > > Thanks everyone. > > > > Jennifer M. Anderson, Scientist > > Halozyme Therapeutics, Inc. > > 11404 Sorrento Valley Road > > San Diego, CA 92121 > > 858-704-8333 > > janderson@halozyme.com > > > > > > > The information transmitted in this email is confidential and is intended > only for the person(s) or entity to which it is addressed. Delivery of > this message to any person other than the intended recipient(s) is not > intended in any way to waive confidentiality or any applicable privilege. > Any review, retransmission, dissemination or other use of, or taking of > any action in reliance upon, this information by individuals or entities > other than the intended recipient is prohibited by Halozyme and may be in > violation of applicable laws. If you received this in error, please > contact the sender and delete/destroy this email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Fri Mar 27 12:58:16 2009 From: portera <@t> msu.edu (Amy Porter) Date: Fri Mar 27 12:58:19 2009 Subject: [Histonet] ? About fixation of excessively bloody tissues Message-ID: <13AD0157B5D84B448EF432B6101F5B20@histolab> Does anyone out there know of a reference that states or discusses tissues that a covered with a large amount of excess blood have problems with fixation. I have looked in the reference materials I have here in the lab, however I cannot find anything to support my complaints to a client that state entire mouse organs (whole liver, spleen, lung, heart...) covered in blood thrown into onecassette with to little formalin will not be well preserved. I know garbage in garbage out - I am trying to get rid of the garbage. Thanks in advance for the help. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu From jdhisto <@t> yahoo.com Fri Mar 27 13:04:33 2009 From: jdhisto <@t> yahoo.com (Jonathan De La Rosa) Date: Fri Mar 27 13:04:36 2009 Subject: [Histonet] Um...Ok! Message-ID: <381058.75911.qm@web110713.mail.gq1.yahoo.com> Wow. Sorry to say it but this whole manual comeback thing is pretty crazy. Talk about panties in a bunch. How about switching to decaf! Histonet is a great resorce for individuals to come for help and/or discussions for ceratin things including answers, comments and well... I would think sometimes manuals and things of that sort. We really should not have to be intimidated or TOTALLY insulted by others.? ? How about next time...try not?opening the email if your going to respond in such a manner. Hopefully from now on you wont check your e.mail?after getting upset at something else, then directing it towards your keyboard in RUDE words?on histonet. JD ?HT (ASCP) From alyssa <@t> alliedsearchpartners.com Fri Mar 27 13:11:29 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Mar 27 13:11:33 2009 Subject: [Histonet] TGIF-Permanent Histotech Job In Central Wisconsin Message-ID: TGIF! I have an opportunity for a full time/permanent histotechnologist/histotechnician in *Central Wisconsin.* Please forward this to anyone you know who may seem fit for this position, and if we place on of your referrals in a position you will earn a $1000 referral bonus. If you are interested the first step would be to please forward your resume to: Alyssa@alliedsearchpartners.com Exprience and Education: High school graduate with a background in biology and chemistry and successful completion of an accredited program in histologic technique required. College coursework desirable. HT ASCP registry or eligible. CPR certified. Minimum one year of experience in a Histology Lab preferred. Job Description: The Histology Technician will assist the physician during the grossing of specimens specifically for the MOHS procedures in the Dermatology Department. The Tech will process, embed, cut and stain tissue specimens so a quality microscopic slide can be produced for diagnosis by a physician/pathologist. Benefits: Generous compensation and benefits package that includes a 401(k) with immediate vesting. Shift: Monday-Friday, 8am-4:30pm. -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From cathy <@t> wasatchhisto.com Fri Mar 27 13:31:26 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Mar 27 13:31:53 2009 Subject: [Histonet] more plastic slides Message-ID: <41B024DF510E4E9CAED8BAECBDB3FD33@shop1e2e996aa5> found some more plastic slides 2" x 4" x 060" clear 164 slides These we used to true the head on the Exakt grinder. Cathy Mayton Wasatch Histo Consultants, Inc. From cathy <@t> wasatchhisto.com Fri Mar 27 13:34:32 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Mar 27 13:34:52 2009 Subject: [Histonet] Free MMA embedding molds Message-ID: Free to good home MMA embedding molds with prepolymerized layers. Different sizes. Just pay for ground shipping. Cathy A. Mayton Wasatch Histo Consultants, Inc. From b-frederick <@t> northwestern.edu Fri Mar 27 13:39:06 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Mar 27 13:39:25 2009 Subject: [Histonet] TMA tape sectioning system Message-ID: <1DE8A2CD888240C9A23B7466C9CFBF90@lurie.northwestern.edu> Does anyone out there remember who had the nifty sectioning tape for TMA's? Or does anyone use it? The vendor was at the NSH meeting last fall. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From NMargaryan <@t> childrensmemorial.org Fri Mar 27 14:38:16 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Mar 27 14:38:43 2009 Subject: [Histonet] Trypsin versus other AR-s Message-ID: <6A2230BAC92E3B4084DAE06869B89FB601EEFA14@CMHEXC01EVS.childrensmemorial.org> Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira From SwainFrancesL <@t> uams.edu Fri Mar 27 14:53:03 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Mar 27 14:53:45 2009 Subject: [Histonet] RE: Trypsin versus other AR-s In-Reply-To: <6A2230BAC92E3B4084DAE06869B89FB601EEFA14@CMHEXC01EVS.childrensmemorial.org> References: <6A2230BAC92E3B4084DAE06869B89FB601EEFA14@CMHEXC01EVS.childrensmemorial.org> Message-ID: I use Trypsin only as a last resort. I use Pepsin because it is more gentle (does not chew up the sections). I do not use Proteinase K unless I absolutely have to and for just a few minutes as it will eat the sections off of the slides. If I use Citrate Buffer I heat it up in the microwave first then quickly put the slides in it cover it and let it sit on the bench top until cool about 20 minutes. As to why we use Citrate buffer there has been several discussions about that on the histonet. You might want to search the archieves and see what others have had to say. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 27, 2009 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trypsin versus other AR-s Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From christiegowan <@t> msn.com Fri Mar 27 15:21:31 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Mar 27 15:21:34 2009 Subject: [Histonet] TMA tape sectioning system In-Reply-To: <1DE8A2CD888240C9A23B7466C9CFBF90@lurie.northwestern.edu> References: <1DE8A2CD888240C9A23B7466C9CFBF90@lurie.northwestern.edu> Message-ID: The tape is made by instrumedics and the system is the cryojane tape transfer system. Christie > From: b-frederick@northwestern.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 27 Mar 2009 13:39:06 -0500 > Subject: [Histonet] TMA tape sectioning system > > > > Does anyone out there remember who had the nifty sectioning tape for TMA's? > Or does anyone use it? The vendor was at the NSH meeting last fall. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Northwestern University > > Pathology Core Facility > > ECOGPCO-RL > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Mar 27 15:22:16 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Mar 27 15:23:54 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> Message-ID: <49CD3578.4040504@umdnj.edu> I, for one, love how he begins all politely with "hopefully not too offensive to those that may be sensitive to such replies" before getting straight to "Are you crazy?" not even three paragraphs later. This is classic trolling at its finest... congratulations Ford! I'll be sure to keep your rant in my inbox so that the next time a pet-peeve of mine comes across the list (i,e: "please unsubscribe me!") I can refer to yours as a good example of "how not to reply to such a huge, helpful list". Lol... froyer@bitstream.net wrote: > Dear Jennifer and any other Histonetters that are of like mind, > > It's not quite "Flaming Friday" yet, but it will be in just a few hours, > so I'll go ahead a little early with what I hope is a reply that may > enlighten, be consultative, and hopefully not too offensive to those that > may be sensitive to such replies. Here it goes... > > My First Question: You state that your lab has ?purchased all used > equipment?. What vendor did you purchase from that did not provide > operator?s manuals with the equipment? This is unheard of amongst us > reputable used/refurbished equipment dealers so if I were you, I would not > recommend this vendor to others. > > Now let?s make sure that I have the facts straight... You have posted a > request that is seeking charity from members of this list that also > includes vendors. In addition, not only are you asking for free copies of > publications that have been copyrighted and are the proprietary > information of the manufacturers that, at great R&D expenditure developed > them, but you are asking that the donor(s) mail hard copies (if available) > to you ...at the donor?s expense for the shipping & handling. Should > hard-copies not be available, you request the donor(s) to provide these > lengthy documents in digital PDF format... again with a substantial > expense to the donor(s) of converting these publications into digital > form. Surprisingly, you have made this burdensome and expensive request > without any offer to reimburse the charitable donor(s) for their time and > expense to do all of this work for you. In your final statement you then > wistfully hope that some benevolent vendor should raise their hand and > offer you a (free) copy and then insult that same vendor buy stating that > you ?won?t hold your breath for that?. > > My Second Question: Are you crazy? > > >From your signature address; it appears that you are employed by a company > called Halozyme Therapeutics. A quick web search informs us that Halozyme > is a for-profit California company that had revenues in the tens of > millions in 2008. It was also exciting to learn that Halozyme is in Phase > 2 clinical trials of their innovative insulin-PH20 with individuals that > suffer from Type 1 diabetes. Very impressive. > > Here is some good news for you. I happen to have ALL the operator manuals > for the equipment that you are requesting. I will copy them for you and > send them to you at my expense under one condition. You state that if > someone can do this for you that you ?will repay the favor, if at all > possible?. I have a family member that is a Type 1 insulin-dependent > diabetic. All I ask in return is that you arrange with your marketing > department that they send me a one-year?s supply of PH20 when it is > released to the public... all at no charge, including shipping & handling > of course. > > My Third Question: Do we have a deal? > > I look forward to your reply, > > ~ Ford > > Ford ?the demon vendor? Royer, MT(ASCP) > Histology Product Manager > Minnesota Medical, Inc. (a for-profit corporation just like yours) > Golden Valley, MN > > > >> Hello Everyone. >> >> >> >> I our lab we've purchased all used equipment. None of these instruments >> came with a user's manual. I am in need of a manual (hard-copy or PDF) >> for the following: >> >> >> >> Sakura Tissue-Tek VIP 3000 >> >> Leica Jung Histo Embedder >> >> Microm HM335E Microtome >> >> >> >> I do realize that requesting a copy of these is a lot to ask of someone. >> It takes a lot of time to copy a 50-page manual. I'll repay the favor, >> if at all possible. I'm hoping that a vendor may raise their hand and >> offer a copy? I won't hold my breath for that... >> >> >> >> Thanks everyone. >> >> >> >> Jennifer M. Anderson, Scientist >> >> Halozyme Therapeutics, Inc. >> >> 11404 Sorrento Valley Road >> >> San Diego, CA 92121 >> >> 858-704-8333 >> >> janderson@halozyme.com >> >> >> >> >> >> >> The information transmitted in this email is confidential and is intended >> only for the person(s) or entity to which it is addressed. Delivery of >> this message to any person other than the intended recipient(s) is not >> intended in any way to waive confidentiality or any applicable privilege. >> Any review, retransmission, dissemination or other use of, or taking of >> any action in reliance upon, this information by individuals or entities >> other than the intended recipient is prohibited by Halozyme and may be in >> violation of applicable laws. If you received this in error, please >> contact the sender and delete/destroy this email. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mucram11 <@t> comcast.net Fri Mar 27 15:36:33 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Mar 27 15:36:36 2009 Subject: [Histonet] TMA tape sectioning system In-Reply-To: Message-ID: <412817585.325881238186193449.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> There is also a paraffin transfer system now it is all from Leica.? You can use the paraffin system with the slides for frozen sections and it works well if you don't have the money for the whole Cryo Jane system.? They purchased the company that bought Instrumedics last year.? It?was under McCormick Scientific prior to the Leica buy out. Pam Marcum ----- Original Message ----- From: "CHRISTIE GOWAN" To: b-frederick@northwestern.edu, histonet@lists.utsouthwestern.edu Sent: Friday, March 27, 2009 4:21:31 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] TMA tape sectioning system The tape is made by instrumedics and the system is the cryojane tape transfer system. Christie ? > From: b-frederick@northwestern.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 27 Mar 2009 13:39:06 -0500 > Subject: [Histonet] TMA tape sectioning system > > > > Does anyone out there remember who had the nifty sectioning tape for TMA's? > Or does anyone use it? The vendor was at the NSH meeting last fall. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Northwestern University > > Pathology Core Facility > > ECOGPCO-RL > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From matthewtclose <@t> gmail.com Fri Mar 27 16:01:06 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Fri Mar 27 16:01:10 2009 Subject: [Histonet] re: difference in hematoxylins Message-ID: <6abc767b0903271401w1c067057l1edbd87eb57eb667@mail.gmail.com> So, there are tons of hematoxylin recipes out there, and many, I find, are very similar in their staining properties. Yes, Richard Allen manufactures a modification of Harris' Hematoxylin, which is essentially Harris' recipe without the mercuric oxide. I don't know how long Richard Allen's variety of this stain keeps, but the traditional Harris' Hematoxylin was only good for a few months. I typically go with either Delafield's, Mayer's (Lillie Mod.) or Ehrlich's Hematoxylin for general H&E staining. These solutions can be used for progressive or regressive techniques and keep for several years. My advice would be to sample a few bottles and see which one works best for you. Or, you could give a hand at making your own. Hematoxylin is very easy to make from essentially hematoxylin, alum (potassium or ammonium), glycerin, sodium iodate, distilled water, alcohol and acetic acid. From shive003 <@t> umn.edu Fri Mar 27 17:47:53 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Mar 27 17:47:58 2009 Subject: [Histonet] Trypsin versus other AR-s References: <6A2230BAC92E3B4084DAE06869B89FB601EEFA14@CMHEXC01EVS.childrensmemorial.org> Message-ID: <2FBB5460073D4D789AF7F7E45033F25E@auxs.umn.edu> Hi Naira, What one uses to unmask an epitope is no one answer. When optimizing a new antibody, you should be trying all the various kinds of ARs available and comparing the results. It's all dependent upon the antigen, where it's located, how difficult it is to unmask, etc. Some methods of antigen retrieval actually can damage your epitope and make it unavailable for detection. When I get a new Ab in, I try multiple dilutions with: -No pretreatment -Enzyme digestion (I use Proteinase K, but have used the other enzymes in the past) -HIER (autoclave or microwave) with citrate buffer pH 6.0 -HIER (autoclave or microwave) with Target Retrieval Solution containing EDTA -HIER (autoclave or microwave) with Target Retrieval Solution at pH 9.9 There are other methods which can be employed as well. (One DOES have to draw the line somewhere!) There's no set consistency between similar types of antigens either, as to what AR they would need. For instance, I stain for maybe 20 different viruses. Some require enzyme digestion, some require HIER (with different kinds of retrieval solutions). But I only figure this out by trying all of the possibilities and comparing. I would not solely depend upon the AR recommendations stated in Ab data sheets. Try them all; you may have better results with some other type of AR than they suggest (I have). So, don't limit your lab's AR to one type. You could be missing much detection of antigen by using the wrong AR method. However, it does require you to multitask, if your lab has a heavy workload and you have to perform AR outside of the autostainer. Most days I'm running HIER with 3 different types of retrieval solutions, as well as doing enzyme digestion at the same time on other slides. Jan Shivers U of MN Vet Diag Lab ----- Original Message ----- From: "Margaryan, Naira" To: Sent: Friday, March 27, 2009 2:38 PM Subject: [Histonet] Trypsin versus other AR-s Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Paul.Panigiris <@t> imvs.sa.gov.au Fri Mar 27 18:11:19 2009 From: Paul.Panigiris <@t> imvs.sa.gov.au (Paul Panigiris) Date: Fri Mar 27 18:11:24 2009 Subject: [Histonet] Hours of operation Message-ID: <000a01c9af31$566de230$641e0a0a@had.sa.gov.au> I was wondering how many Histo labs in Australia are working on a 24 hour system. What have they seen as the advantages but more importantly the disadvantages with such a system. P From talulahgosh <@t> gmail.com Fri Mar 27 21:13:27 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Mar 27 21:13:30 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <49CD3578.4040504@umdnj.edu> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> <49CD3578.4040504@umdnj.edu> Message-ID: I thought the rant was pretty funny. Just repeat to yourself "it's an email list, I should really just relax." Emily -- prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves From gu.lang <@t> gmx.at Sat Mar 28 03:56:04 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 28 03:56:16 2009 Subject: AW: [Histonet] Staining vs. paraffin wax problems In-Reply-To: References: Message-ID: <8382C513E76C4276B3B833DD73E48C84@dielangs.at> Does this look like desert-earth cracks? If yes, this could be a matter of water under the edges after sectioning and half-drying. Do you find the artefacts in the lower part of your sections related to the drying-direction (upright)? The section dries and sticks from the outer paraffin-limits to the center. When the rest of the section sits down and shrinks, it could happen that it tears. (Seen as a water-filled blister.) Be sure, that the water is thrown out before drying, and dry vertically. The other possibility is a too hot waterbath. The sections expand too much, but I think this would be seen all over the section. This can also be seen, if the paraffin infiltration wasn't sufficient. I hope my English is good enough to explain my thoughts. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jason McGough Gesendet: Freitag, 27. M?rz 2009 17:27 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Staining vs. paraffin wax problems We are experiencing difficulties with our H&E staining and other Special Stains. After the slides are stained the edges of the tissue look fragmented/chattered. Our pathologists also thought the sections looked like they might be too thick but we checked all of our microtomes for thickness settings and all are set at 4 micrometers. There seems to be several blotches of light staining throughout the tissue. This seems to be only on our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene. Is this a deparaffinization, processing, or a staining issue? Any help is appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Mar 28 04:06:04 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 28 04:06:12 2009 Subject: AW: [Histonet] Trypsin versus other AR-s In-Reply-To: <6A2230BAC92E3B4084DAE06869B89FB601EEFA14@CMHEXC01EVS.childrensmemorial.org> References: <6A2230BAC92E3B4084DAE06869B89FB601EEFA14@CMHEXC01EVS.childrensmemorial.org> Message-ID: <7EC926516C8F4C75957844A074AFD630@dielangs.at> If PIER is needed, we use Proteinase K, but we avoid it, if possible. Citrate Buffer is historically the next buffer, that got generally used. Few years later with investigations about Ca-Ions involved in fixation, the EDTA-buffers with high pH appeared. It was shown, that most of the diagnostic antibodies work well with high pH retrieval. So my first choice is the EDTA-buffer when introducing a new antibody. Although the most datasheets require Citrate buffer. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Margaryan, Naira Gesendet: Freitag, 27. M?rz 2009 20:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Trypsin versus other AR-s Hi Dears, It is Friday, but not a weekend yet:-):-( I just got a request from my PI to figure out: 1. How often now day's people use Trypsin (EDTA, Proteinase K or E) as an Antigen Retrieval for FFPE. 2. Why or is the Citrate Buffer pH6 more usable??? 3. Is Trypsin very old technique? Any educational feedback is appreciated, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sat Mar 28 08:05:15 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sat Mar 28 08:05:19 2009 Subject: [Histonet] Re: difference in hematoxylins Message-ID: I think we've gone over the terminology of alum hematoxylins more than once on Histonet. When you make up a working solution of what we call hematoxylin, you dissolve the colorless dyestuff (dye precursor) hematoxylin in water. With oxidation the colorless dyestuff becomes the dye, called hematein (though this word is rarely used). The hematein deteriorates, forming the iridescent scum on the surface and the precipitate that adheres to the glass. The aluminum (alum) serves as a mordant, attaching the dye to the material to be dyed. Glycerol, ethanol, chloral hydrate, ethylene glycol, and similar additives serve primarily as preservatives. The acid (traditionally acetic) determines a lot of the staining qualities of the mix, and determines whether a regressive step (partial decolorization in acid after hematoxylin staining. Do NOT put this in my frozen section sequence!) is needed. How the oxidation is done determines the usual terminology of hematoxylins. Delafield's hematoxylin: loosely stopper the newly prepared hematoxylin and leave it under the sink for a few months. Atmospheric oxygen slowly accomplishes the oxidation. Harris's hematoxylin: heat the solution almost to boiling and add mercuric oxide. Mayer's hematoxylin: oxidize at room temperature with sodium iodate. (Mayer rimes with liar, not with layer.) Because of present day restrictions on mercury in the laboratory, Harris's hematoxylin is probably out of manufacture, and making it yourself is not a good idea. The new Good Management definition of Harris's hematoxylin is "any alum hematoxylin our marketing department wants to call Harris hematoxylin." It's sometimes labeled as not containing mercury. The Gill hematoxylins are (or once were) Mayer-type hematoxylins, with a rationalized recalculation of the formula that goes back to J.T. Baker (Cytologic Technique, 1960, if I remember correctly). If Gary Gill is still on Histonet, he can elaborate. Proprietary hematoxylins today are probably oxidized either with sodium iodate or with oxygen. This doesn't matter to the user. With any brand of hematoxylin that's new to you, you have to work out a staining sequence that works for you. This should be done with the input of your pathologist or researcher. Unfortunately, most pathologists and researchers know very little about these matters, and information on the subject isn't easy for them to get. Bob Richmond Samurai Pathologist Knoxville TN From enrriq88 <@t> yahoo.com Sat Mar 28 09:41:46 2009 From: enrriq88 <@t> yahoo.com (Jaime Plata) Date: Sat Mar 28 09:41:51 2009 Subject: AW: [Histonet] Staining vs. paraffin wax problems In-Reply-To: <8382C513E76C4276B3B833DD73E48C84@dielangs.at> Message-ID: <628842.29643.qm@web50408.mail.re2.yahoo.com> Very good, Gudrun I agree with you. I have a seen this crack also on the blocks after embedding them on the cooling stage, and you separate the cassette from the mold.? On the tissue and the paraffin, you will see this type of cracking description that you mentioned.? In this case I will describe it as Ice (paraffin)-warming crack. Please correct me if I am wrong. This term that I used is some type of global warming reaction phenomenon in paraffin. --- On Sat, 3/28/09, Gudrun Lang wrote: From: Gudrun Lang Subject: AW: [Histonet] Staining vs. paraffin wax problems To: "'Jason Mcgough'" Cc: histonet@lists.utsouthwestern.edu Date: Saturday, March 28, 2009, 4:56 AM Does this look like desert-earth cracks? If yes, this could be a matter of. Water under the edges after sectioning and half-drying. Do you find the? Artefacts in the lower part of your sections related to the drying-direction. (upright)? The section dries and sticks from the outer paraffin-limits to the center. When the rest of the section sits down and shrinks, it could happen that it. tears. (Seen as a water-filled blister.) Be sure, that the water is thrown out before drying, and dry vertically. The other possibility is a too hot waterbath. The sections expand too much, But I think this would be seen all over the section. This can also be seen, if the paraffin infiltration wasn't sufficient. I hope my English is good enough to explain my thoughts. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsbounces@lists.utsbounces@lists.utsouthwestern.eduI'mAuftrag von Jason. Mcgough Gesendet: Freitag, 27. M?rz 2009 17:27 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Staining vs. paraffin wax problems We are experiencing difficulties with our H&E staining and other Special Stains. After the slides are stained the edges of the tissue look. fragmented/chattered. Our pathologists also thought the sections looked like. They might be too thick, but we checked all of our microtomes for thickness. Settings and all are set at 4 micrometers. There seems to be several. Blotches of light staining throughout the tissue. This seems to be only on. Our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene. Is this a deparaffinization, processing, or a staining issue? Any help is. appreciated. Jason Mcgough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Mar 28 10:08:41 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 28 10:08:45 2009 Subject: [Histonet] ? About fixation of excessively bloody tissues In-Reply-To: <13AD0157B5D84B448EF432B6101F5B20@histolab> Message-ID: <539987.30938.qm@web65708.mail.ac4.yahoo.com> The problem has nothing to do with the organs being blood covered but with the fact that they are entire and with little formalin. If they?were sliced to expose the interior and placed in enough formalin it is of no importance how covered with blood externally they are. Ren? J. --- On Fri, 3/27/09, Amy Porter wrote: From: Amy Porter Subject: [Histonet] ? About fixation of excessively bloody tissues To: "Histonet" Date: Friday, March 27, 2009, 1:58 PM Does anyone out there know of a reference that states or discusses tissues that a covered with a large amount of excess blood have problems with fixation. I have looked in the reference materials I have here in the lab, however I cannot find anything to support my complaints to a client that state entire mouse organs (whole liver, spleen, lung, heart...) covered in blood thrown into onecassette with to little formalin will not be well preserved. I know garbage in garbage out - I am trying to get rid of the garbage. Thanks in advance for the help. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Sat Mar 28 10:16:12 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sat Mar 28 10:16:17 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> <49CD3578.4040504@umdnj.edu> Message-ID: <5b6eb13e0903280816x338f25b6iee66734ecb9b89@mail.gmail.com> It was pretty funny alright. One for the Histonet Posts of Shame. There are a few of us that could be runners-up with Ford. haha Mark On Fri, Mar 27, 2009 at 7:13 PM, Emily Sours wrote: > I thought the rant was pretty funny. > > Just repeat to yourself "it's an email list, I should really just relax." > > > Emily > -- > prometheus, thief of light, giver of light, bound by the gods, must have > been a book. > -mark danielewski, house of leaves > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From drvet_anjan <@t> hotmail.com Sat Mar 28 15:47:28 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Sat Mar 28 15:47:32 2009 Subject: [Histonet] Vector VIP- Vector Blue- HRP &AP polymer secondary antibody Message-ID: hi , I wanted to know what would happen if i use Vector Blue and Vector VIP chromogen substrate with Labvision's HRP and Alkaline phosphatase polymer secondary antibody. Since these chromogens come as avidin biotin cojugated enzymes which may cause reaction with endogenous Biotin. Plz Advice. Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx From dmlaud <@t> gmail.com Sat Mar 28 21:23:00 2009 From: dmlaud <@t> gmail.com (Damien) Date: Sat Mar 28 21:23:05 2009 Subject: [Histonet] Re: Cortical bone image analysis fluorescent calcein measurement (Damien Laudier) Message-ID: <6f5a847e0903281923j6de1c381rc8c8d3312b3f7602@mail.gmail.com> Hi Jamie, Goal 1: For fluorescent label measurements, you?re definitely better off measuring multiple fields using a higher power objective (at least10X). Accuracy is paramount when measuring inter-label width and working at a higher magnification will help to ensure this. Your other option is to create a photo-stitched composite image of each sample (you would take multiple images until you get the entire circumference of the sample) and make measurements from a static image. Osteometrics *Osteomeasure* software is not ?overkill? and is considered the simpler of the two popular bone histomorphometry packages on the market. The beauty of using such programs specifically designed for bone histomorphometry is that the calibration standards/code are already established/programmed, as well as the back-end calculations required to derive MAR. You can certainly make these measurements using Zeiss axiovision software or any imaging software (NIH Image J, Matlab IPT). However, you?ll need to establish your own parameters and this will require more derivative calculations on your part after principle data collection; a great option if you?re comfortable doing this. Goal 2: If you?re using your (expensive) precision saw correctly, you should not be getting uneven sections. Try reducing your cutting speed and/or adjusting the angle of your sample. Also, make sure you?re using a blade of the proper size- relative to the size of the sample. Use a micrometer to gauge your cutting adjustments. Once you fix this problem, yes it is possible on a 1mm section, but since all confocal microscopes are not created equally, success will ultimately depend on the resolving power of your system. Good luck! -Damien Laudier From ratliffjack <@t> hotmail.com Sat Mar 28 22:33:21 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Sat Mar 28 22:33:51 2009 Subject: [Histonet] Re: Cortical bone image analysis fluorescent calcein measurement (Damien Laudier) In-Reply-To: <6f5a847e0903281923j6de1c381rc8c8d3312b3f7602@mail.gmail.com> References: <6f5a847e0903281923j6de1c381rc8c8d3312b3f7602@mail.gmail.com> Message-ID: I will add to Damien's comments and say that another thing to consider is the labeling schedule. Typically the smaller the animal the greater interlabel time period. For example, a smaller window in a mouse tibia/ femur might make it difficult to determine the difference between single and double labels. On the other hand, the remodeling rate can also affect this where in say a larger animal like a dog or sheep with a greater interlabel time period could make you miss your double labels because your first label could be resorbed. Lastly, you are better served with your mouse and rat sections if you can grind your sections thinner at say 20-30 microns. This essentially will sharpen the labels so that a single label can not be mistaken for a double label due to a halo effect from label over-expression. You can accomplish this goal either automatically with say an Exakt grinder or manually with a suction cup and a little patience. Regardless, they are all very teachable methods. I will again encourage you attend the BIOQUANT Image Analysis Meeting April 21-23 in Nashville as all of these topics will be addressed in detail from a histology, histopatholgy, and image analysis perspective. If you can attend, I also encourage you to bring a representative polymerized specimen and/or prepared slides. Jack On Mar 28, 2009, at 10:23 PM, Damien wrote: > Hi Jamie, > > Goal 1: > > For fluorescent label measurements, you?re definitely better off mea > suring > multiple fields using a higher power objective (at least10X). > Accuracy is > paramount when measuring inter-label width and working at a higher > magnification will help to ensure this. Your other option is to > create a > photo-stitched composite image of each sample (you would take multiple > images until you get the entire circumference of the sample) and make > measurements from a static image. Osteometrics *Osteomeasure* > software is > not ?overkill? and is considered the simpler of the two popular bo > ne > histomorphometry packages on the market. The beauty of using such > programs > specifically designed for bone histomorphometry is that the > calibration > standards/code are already established/programmed, as well as the > back-end > calculations required to derive MAR. You can certainly make these > measurements using Zeiss axiovision software or any imaging software > (NIH > Image J, Matlab IPT). However, you?ll need to establish your own par > ameters > and this will require more derivative calculations on your part after > principle data collection; a great option if you?re comfortable doin > g this. > > Goal 2: > > If you?re using your (expensive) precision saw correctly, you should > not be > getting uneven sections. Try reducing your cutting speed and/or > adjusting > the angle of your sample. Also, make sure you?re using a blade of th > e proper > size- relative to the size of the sample. Use a micrometer to gauge > your > cutting adjustments. Once you fix this problem, yes it is possible > on a 1mm > section, but since all confocal microscopes are not created equally, > success > will ultimately depend on the resolving power of your system. > > Good luck! > > -Damien Laudier > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From olvluis <@t> hotmail.com Sun Mar 29 10:15:17 2009 From: olvluis <@t> hotmail.com (jose olvera) Date: Sun Mar 29 10:15:26 2009 Subject: [Histonet] swicth my email address Message-ID: Dear Sir/Madam: Please change my old e-mail address(olvluis@hotmail.com) to my new e-mail: JoDr306@gmail.com as soon as possible I thank you in advance. Jose olvera HT. _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From matthewtclose <@t> gmail.com Sun Mar 29 14:41:03 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Sun Mar 29 14:41:08 2009 Subject: [Histonet] re:paraffin and staining problems Message-ID: <6abc767b0903291241k5735569dkd57a043e25ae5dad@mail.gmail.com> I think that your problem has mostly to do with inadequate dehydration/infiltration. I've had this problem mainly with sections of vertebrate skin or sections that possess lots of little blood vessels. Water is either unable to move through the keratin sufficiently to dehydrate and I often acquire embedding/sectioning problems as a result. With the blood vessels, I think the problem is more of tissue thickness and heterogeneity. The thicker and more heterogeneous tissues require longer dehydration and infiltration times, and since I prefer xylene over cedarwood oil, I have to play the game of hard tissue vs. inadequate infiltration. I would perhaps try an extra fresh absolute ethanol step and add a fresh xylene every few days/rounds or so. At some point I figured out the right combo. But I still come across similar problems to what you are having, at least with the sections cracking. If all of the water isn't removed, you'll get expansion/contraction at different rates than the paraffin this resulting in cracks in the block (usually upon cooling). I will usually go back and reinfiltrate. With the poor staining, it sounds like a post infiltration problem, maybe even a post sectioning problem. How are you mounting sections? -Matt From roqroq2000 <@t> yahoo.com Sun Mar 29 15:16:43 2009 From: roqroq2000 <@t> yahoo.com (Sahar Darwish) Date: Sun Mar 29 15:16:47 2009 Subject: [Histonet] (no subject) Message-ID: <580883.23189.qm@web56706.mail.re3.yahoo.com> dear. respon for histonet: ? ???? i send many message?to other participent in this site ,i have no response, sory iam depressed, i have i question? about how to differentiate between islets ccells of pancreas with special stain ,may have an answer please. ? ? ?Dr .sahar kamal ? ??? researcher in histology From rjbuesa <@t> yahoo.com Sun Mar 29 15:50:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 29 15:51:01 2009 Subject: [Histonet] (no subject) In-Reply-To: <580883.23189.qm@web56706.mail.re3.yahoo.com> Message-ID: <114107.1539.qm@web65711.mail.ac4.yahoo.com> Sahar Darwish: A similar?question was posted several days ago and I recommended then, as I do now, to use the Mallory-Azan technique. I hope that you get an answer now. Ren? J. --- On Sun, 3/29/09, Sahar Darwish wrote: From: Sahar Darwish Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Sunday, March 29, 2009, 4:16 PM dear. respon for histonet: ? ???? i send many message?to other participent in this site ,i have no response, sory iam depressed, i have i question? about how to differentiate between islets ccells of pancreas with special stain ,may have an answer please. ? ? ?Dr .sahar kamal ? ??? researcher in histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Sun Mar 29 17:23:56 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Sun Mar 29 17:24:09 2009 Subject: [Histonet] (no subject) In-Reply-To: <580883.23189.qm@web56706.mail.re3.yahoo.com> References: <580883.23189.qm@web56706.mail.re3.yahoo.com> Message-ID: <49CFF4FC.5020000@vneubert.com> Dear Sahar Darwish, I just had a look into the last 3330 mails I received from this list so far but could not find any mail sent by you. Please make sure you send your requests to histonet@lists.utsouthwestern.edu for making sure it is bounced to every participant. Greetings, V. Neubert Sahar Darwish wrote: > dear. respon for histonet: > > i send many message to other participent in this site ,i have no response, sory iam depressed, i have i question about how to differentiate between islets ccells of pancreas with special stain ,may have an answer please. > > > Dr .sahar kamal > > researcher in histology > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From yvan_lindekens <@t> yahoo.com Sun Mar 29 23:46:46 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Sun Mar 29 23:46:50 2009 Subject: [Histonet] searching instruction manuals for Shandon Histocentre 2 and Medite COT20 Message-ID: <863665.68598.qm@web110204.mail.gq1.yahoo.com> Hi all, If anyone here would happen to have an instruction manual for a Shandon Histocentre 2 and/or a Medite COT20 linear slide stainer, I would be very gratefull for a xerox/PDF copy. Of course I'm willing to pay the costs, but I cannot deliver free medication in return, sorry. Thanks in advance, Yvan. From ree3 <@t> leicester.ac.uk Mon Mar 30 06:22:00 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Mar 30 06:22:07 2009 Subject: [Histonet] B cell markers Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783C5A@EXC-MBX3.cfs.le.ac.uk> Wanted a B cell marker that works on paraffin or GMA processed human tissues, any ideas please?. Cheers Richard Edwards University of Leicester U.K. From PVerden <@t> UROPARTNERS.COM Mon Mar 30 08:49:30 2009 From: PVerden <@t> UROPARTNERS.COM (Paul Verden) Date: Mon Mar 30 08:49:41 2009 Subject: [Histonet] owners manuals Message-ID: Personally, I would like to thank Ford "the demon vendor" Royer at Minnesota Medical, Inc.for his extensive dissertation on the request for assistance on finding manuals. Our lab will be expanding in the near future and will be in need of some used equipment. It's good to know the equipment vendors you would like to avoid. R. Paul Verden From c.m.vanderloos <@t> amc.uva.nl Mon Mar 30 09:17:10 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Mar 30 09:17:25 2009 Subject: [Histonet] Re: Vector VIP- Vector Blue- HRP &AP polymer secondary antibody In-Reply-To: References: Message-ID: Hello Anjan Kumar, Vector VIP, Vector Blue, yields purple and clear blue reaction products, respectively. That it self is certainly not an ideal combination for double staining, simply because these chromogens does not give much contrast. In case of co-localization there will be a problem recognizing the mixed-color from the basic colors. In this respect, Vector NovaRed and Vector Blue is a much better chromogen combination. What exactly do you mean with '...these chromogens come as avidin biotin conjugated enzymes...'? When working with the Labvision HRP and AP polymer secondary reagents, there is no avidin nor biotin involved. To my terminology the chromogen is the colored reaction endproduct that is visible under the microscope. Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands ----- Original Message ----- From: anjan kumar Date: Saturday, March 28, 2009 9:47 pm Subject: Vector VIP- Vector Blue- HRP &AP polymer secondary antibody To: triple immunohistochem , c.m.vanderloos@amc.uva.nl > hi , > I wanted to know what would happen if i use Vector Blue and > Vector VIP chromogen substrate with Labvision's HRP and Alkaline > phosphatase polymer secondary antibody. Since these chromogens come as > avidin biotin cojugated enzymes which may cause reaction with > endogenous Biotin. > Plz Advice. > > Dr. Anjan Kumar.K.R > > M.V.Sc Scholar > > Dept. of Veterinary Pathology > > Madras Veterinary College > > Chennai-7 > > India > > email: drvet_anjan@hotmail.com > > Phone: +91-9940475801 > > > > _________________________________________________________________ > How fun is this? IMing with Windows Live Messenger just got better. > http://www.microsoft.com/india/windows/windowslive/messenger.aspx From mpence <@t> grhs.net Mon Mar 30 09:27:18 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Mar 30 09:27:26 2009 Subject: [Histonet] Cyto prep question Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3AF3@IS-E2K3.grhs.net> I know this many not be the forum to ask this question, but I know many of us have cyto depts. within our labs. We have been seeing an increase in the number of unsatisfactory urine specimens from our Urology group lately and it is due to lubricant on the ThinPrep filter. We have worked with the Urology group and they are using the recommended lubricant from the manufactory. We see the problem even when the urine is diluted in CytoLyt for several hours. Has anyone else experienced this problem? Thanks in advance, Mike From jcampbell <@t> vdxpathology.com Mon Mar 30 09:44:10 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Mon Mar 30 09:44:13 2009 Subject: [Histonet] pressure cookers for IHC Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF81DF732@VDXSERVER01.vdxpathology.local> Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type of pressure cooker to use? Protocol used as far as the temp and for how much time? Any info would be appreciated. Thanks, Jen C. From shive003 <@t> umn.edu Mon Mar 30 09:47:10 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Mar 30 09:47:15 2009 Subject: [Histonet] B cell markers References: <7722595275A4DD4FA225B92CDBF174A17457783C5A@EXC-MBX3.cfs.le.ac.uk> Message-ID: <966FBF38AAE449C99652C4F4F335457D@auxs.umn.edu> On FFPE tissue - CD20 LabVision/NeoMarkers cat. # RB-9013 Rabbit polyclonal Does not need any tissue pretreatment Works GREAT. Jan Shivers Univ. of Minnesota Vet Diag Lab ----- Original Message ----- From: "Edwards, R.E." To: Sent: Monday, March 30, 2009 6:22 AM Subject: [Histonet] B cell markers Wanted a B cell marker that works on paraffin or GMA processed human tissues, any ideas please?. Cheers Richard Edwards University of Leicester U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Mar 30 09:50:13 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Mar 30 09:50:28 2009 Subject: [Histonet] Please help! In dire need of user manuals In-Reply-To: <5b6eb13e0903280816x338f25b6iee66734ecb9b89@mail.gmail.com> References: <2114.216.243.132.54.1238122245.squirrel@webmail.iphouse.com> <49CD3578.4040504@umdnj.edu> <5b6eb13e0903280816x338f25b6iee66734ecb9b89@mail.gmail.com> Message-ID: <6C63AC644438D41AF45A7D8B@bchwxp2702.ad.med.buffalo.edu> I always find it interesting to discover the vast sea of temperaments out there that is elucidated so clearly in the light of email responses. The same email can provoke anger, inflict hurt, or elicit laughter from different people. :-) With that in mind studies have shown (so I'm told by a friend who works in IT here at UB - I don't have the source) that 90% of communication is lost in email. When you don't see facial expression, gesturing, and, most importantly, tone of voice, you may never appreciate the sender's true intent. Especially if the person is someone you've never met, and, most importantly for this listserv, may be coming from another culture or even subculture. I have been caught up in several arguments over personal email (not on this listserv) with people I KNEW that were never meant to escalate as they did, simply because of miscommunication on one or both ends. :-) I am not saying that Mr. Royer was not in an argumentative (or satirical, or sarcastic) state, as key words and phrases from his email that others pointed out indicate he may not have been in the best of moods at that time. And there are people who, if in an especially sensitive mood and are in "dire need of help", can certainly take very personally what has been said by someone they've never met before (both my hands are raised). And there are people who become highly offended for the sake of the offended person. And there are those who see it all quite comically (as I actually did at first until I started re-reading the emails from different perspectives). :-) Just my thoughts! Merced --On Saturday, March 28, 2009 8:16 AM -0700 Mark Tarango wrote: > It was pretty funny alright. One for the Histonet Posts of Shame. There > are a few of us that could be runners-up with Ford. haha > > Mark > > On Fri, Mar 27, 2009 at 7:13 PM, Emily Sours > wrote: > >> I thought the rant was pretty funny. >> >> Just repeat to yourself "it's an email list, I should really just relax." >> >> >> Emily >> -- >> prometheus, thief of light, giver of light, bound by the gods, must have >> been a book. >> -mark danielewski, house of leaves >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From PVerden <@t> UROPARTNERS.COM Mon Mar 30 09:51:10 2009 From: PVerden <@t> UROPARTNERS.COM (Paul Verden) Date: Mon Mar 30 09:51:14 2009 Subject: [Histonet] Cyto prep question In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3AF3@IS-E2K3.grhs.net> Message-ID: Mike - Lubricant is the bane of our existence when it comes to ThinPrep. We process 30 - 70 urine specimens per day, and we have that problem whenever excess lubricant is used. We set up a protocol to use a 1:9 glacial acetic acid:Cytolyt solution as a wash when we encounter the problem. Sometimes it works; most times it doesn't. It will be interesting to find out what other labs have done. Good luck. Paul Verden UroPartners, LLC Westchester, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, March 30, 2009 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyto prep question I know this many not be the forum to ask this question, but I know many of us have cyto depts. within our labs. We have been seeing an increase in the number of unsatisfactory urine specimens from our Urology group lately and it is due to lubricant on the ThinPrep filter. We have worked with the Urology group and they are using the recommended lubricant from the manufactory. We see the problem even when the urine is diluted in CytoLyt for several hours. Has anyone else experienced this problem? Thanks in advance, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Mon Mar 30 09:53:32 2009 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Mon Mar 30 09:53:37 2009 Subject: [Histonet] IHC automated buffers Message-ID: <3809C163DC1DA54AA534B3C7794D07B631ADB55905@ENHBGMBX01.PA.LCL> Hello, Is anyone presently making their own TBS with tween 20 buffer to be used in automated IHC? I would like to know if there are any problems related to these buffers and if any deviations must be made to accommodate the automation. Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! From rfields <@t> gidocs.net Mon Mar 30 09:52:41 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Mon Mar 30 09:55:09 2009 Subject: [Histonet] pressure cookers for IHC References: <5658CBDB9EAE6545ABE50D2563D81BF81DF732@VDXSERVER01.vdxpathology.local> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F6F95A8@GIEXCHANGE.gidocs.net> I am just starting to bring IHC in house to our new lab, I have been working with the tech staff at Cell Marque. They sent me a "cuisinart" pressure cooker to work with, it is pretty nice.. has a digital timer.. gets the job done. I have only been working up H.pylori at this time, with a pre-dilute antibody.. but I have used the pressure cooker at high temp, 15 mins with success. It allows you to pick low/high also.. Much cheaper than other models I looked at. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Monday, March 30, 2009 9:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pressure cookers for IHC Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type of pressure cooker to use? Protocol used as far as the temp and for how much time? Any info would be appreciated. Thanks, Jen C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Mon Mar 30 10:03:23 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Mar 30 10:03:27 2009 Subject: [Histonet] pressure cookers for IHC References: <5658CBDB9EAE6545ABE50D2563D81BF81DF732@VDXSERVER01.vdxpathology.local> Message-ID: <523F834323F54305A93C6B080AB82B4C@auxs.umn.edu> Hi Jen, I think you should first check your quality control governing group rules regarding equipment data verification to see if a store-bought pressure cooker meets the required quality control standards. I'm using Biocare's Decloaking Chamber for my HIER now. Yes, the machine is an expensive piece of equipment, but when we were using a microwave to do the HIER, I could not verify the temperature (I was using a kitchen microwave oven). With the Decloaker, I now have a dial and digital readout which show the pressure/temps, and this gets recorded in a log book every time we use the machine. I must say, using this device has increased my positive staining perhaps double of what I was getting with the microwave... and I thought I was getting strong staining with the microwave! I've had to dilute out my antibodies, which is a good thing. Jan Shivers Univ. of Minnesota Vet Diag Lab ----- Original Message ----- From: "Jennifer Campbell" To: Sent: Monday, March 30, 2009 9:44 AM Subject: [Histonet] pressure cookers for IHC Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type of pressure cooker to use? Protocol used as far as the temp and for how much time? Any info would be appreciated. Thanks, Jen C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Mon Mar 30 10:24:00 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Mar 30 10:24:09 2009 Subject: [Histonet] bovine vs donkey anti-goat IgG Message-ID: Has anyone compared the bovine anti-goat IgG to the donkey anti-goat IgG secondaries from Jackson IR and seen whether there was a considerable benefit to using the bovine series? My BSA is gamma globulin free so it shouldn't make a huge difference for me, but I am just wondering ......... ANDREA -- From pritchm <@t> ccf.org Mon Mar 30 10:27:29 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Mon Mar 30 10:27:41 2009 Subject: [Histonet] pressure cookers for IHC In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF81DF732@VDXSERVER01.vdxpathology.local> Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E0215142C@CCHSCLEXMB56.cc.ad.cchs.net> Hi Jennifer: I wrote this response to Casper Hempel 03 March 2009 when he posted a question regarding the relative importance of temperature and pH in HIER techniques to the Histonet community. I copied and pasted it below (and edited it for grammar, organization and spelling this time!) for your information. I have a reproducibly good method for HIER for two Abs I routinely use (alpha smooth muscle actin in mouse liver and proliferating cell nuclear antigen (PCNA) in mouse liver). I use a microwave pressure cooker (Nordic Ware, tender cooker). Into the pressure cooker, I place 300mL of distilled water to keep the humidity high during the heating process. I use a citrate buffer solution which contains 0.01M sodium citrate dihydrate and 0.04M citric acid monohydrate, pH 6.0. I place 500mL of this solution into a 1.5pt (710mL) 'servin' saver' container and set the filled container into the pressure cooker (into the distilled water). I slip my slides into an autostainer rack and place the rack on its side in the citrate buffer soln. I microwave the entire setup at 50% power (1100 watt microwave) for 20 minutes. When I remove the cooker from the microwave, I open it on my bench and take the temperature of the citrate buffer; it is usually between 95-98C. After the 20 minute cool-down, the temperature of the citrate buffer is around 56-57C. Once completely cool, I test the pH of the citrate buffer to ensure that it is still at pH 6.0. (I have taken these measurements a total of 11 times.) My single failure when using this method of HIER occurred when the temperature did not get hot enough. I was rather na?ve at the time and thought I would proceed with the staining and see what happened, assuming the pH was more important than the temperature. So much for that hypothesis. Mind you, I fully disclose that my experience is rather limited to the two staining protocols I have optimized, but I offer up that experience for your edification. Hope it helps! Kind regards: ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Monday, March 30, 2009 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pressure cookers for IHC Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type of pressure cooker to use? Protocol used as far as the temp and for how much time? Any info would be appreciated. Thanks, Jen C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From micropathlabs <@t> yahoo.com Mon Mar 30 10:30:07 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Mar 30 10:30:11 2009 Subject: [Histonet] Cyto prep question In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3AF3@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3AF3@IS-E2K3.grhs.net> Message-ID: <328124.94685.qm@web57803.mail.re3.yahoo.com> We had the same problem here?and after much?QC to follow trends, found that it was the same two clinicians. They had?2 of 8 exam rooms with the wrong lubricant and also needed?more information on collection. Since then, no further problems. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: Mike Pence To: histonet@lists.utsouthwestern.edu Sent: Monday, March 30, 2009 10:27:18 AM Subject: [Histonet] Cyto prep question I know this many not be the forum to ask this question, but I know many of us have cyto depts. within our labs. We have been seeing an increase in the number of unsatisfactory urine specimens from our Urology group lately and it is due to lubricant on the ThinPrep filter.? We have worked with the Urology group and they are using the recommended lubricant from the manufactory.? We see the problem even when the urine is diluted in CytoLyt for several hours. Has anyone else experienced this problem? Thanks in advance, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Mon Mar 30 10:41:07 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Mar 30 10:41:13 2009 Subject: [Histonet] Etchers? Any that do barcodes on slides and cassettes? Also looking for Dave Low Message-ID: Hey netters, I have a lamb microwriter than in 2005 came with windows 98! So the little disc drive that runs constantly and is noisy and came with one disc is aging and no amount of Botox or collagen will keep it from frowning. So thinking about purchasing another one, and or connecting the existing writers to a regular computer. Anyone out there had any success with this? Also are there any decent writers that will do barcodes on slides and cassettes and make it thru everything? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. > P Please consider the environment before printing this e-mail > To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Shirley.Chu <@t> moldev.com Mon Mar 30 10:55:03 2009 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Mon Mar 30 10:55:14 2009 Subject: [Histonet] California Society for Histotechnology Annual Symposium/Convention Message-ID: The California Society for Histotechnology will be holding its Annual Symposium/Convention on May 14-17, 2009 at the Westin San Francisco Airport (hotel rates $129 for single or double). Our program contains 19 workshops, all which will be CEU/Contact Hour accredited by NSH. The hotel rate will be $129 for a single or double. For hotel reservations, please contact the hotel at: 650-692-3500 or for online registration: http://www.starwoodmeeting.com/StarGroupsWeb/res?id=0806209635&key=F392. Deadline for hotel reservations are April 22, 2009. For a copy of the program, please contact me directly or visit our website at: http://www.californiahistology.org. Information concerning exhibiting at our meeting is also available on our website. Thanks. Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com This e-mail and any files transmitted with it may contain privileged and/or confidential information and may be read or used only by the intended recipient. If you are not the intended recipient of the e-mail or any of its attachments, please be advised that you have received this e-mail in error and any use, dissemination, distribution, forwarding, printing or copying of this e-mail or any attached files is strictly prohibited. If you have received this e-mail in error, please immediately purge it and all attachments and notify the sender by reply e-mail or contact the sender at the number listed. From shive003 <@t> umn.edu Mon Mar 30 12:48:38 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Mar 30 12:48:43 2009 Subject: [Histonet] IHC automated buffers References: <3809C163DC1DA54AA534B3C7794D07B631ADB55905@ENHBGMBX01.PA.LCL> Message-ID: <13EC8A4018C84A82A43104D961F1C2B1@auxs.umn.edu> We mix up our own, using Tween 20 at 0.05% per volume. No problems here, when using a Dako autostainer. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Charles, Roger" To: Sent: Monday, March 30, 2009 9:53 AM Subject: [Histonet] IHC automated buffers Hello, Is anyone presently making their own TBS with tween 20 buffer to be used in automated IHC? I would like to know if there are any problems related to these buffers and if any deviations must be made to accommodate the automation. Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victoria.spoon <@t> bassett.org Mon Mar 30 13:16:01 2009 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Mon Mar 30 13:16:07 2009 Subject: [Histonet] Gross room assistant position available in Cooperstown, NY Message-ID: <415700FC732DE14491A3E39367834F77022278FF@ex3.bassett.org> Bassett Hospital in Cooperstown NY has a gross room assistant position available. Monday through Friday 8:30 am to 5 pm. Responsibilities include receiving/accessioning specimens, gross examination of biopsies working with a full time PA, assisting with frozen sections and general equipment maintenance. A NYS license as a histotechnician or certified lab technician is required or must be eligible. For more information or if you are interested in the position: Go to http://www.bassett.org/ and click on career center Or contact: Victoria Spoon Anatomic Pathology Manager Bassett Hospital Cooperstown NY 13326 victoria.spoon@bassett.org Tel(607)547-6357 Fax(607)547-3203 From MElliott <@t> mrl.ubc.ca Mon Mar 30 15:21:16 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Mon Mar 30 15:21:56 2009 Subject: [Histonet] Region IX Education Day Message-ID: <49D0C74C.11C6.00D6.0@mrl.ubc.ca> Region IX is proud to announce that their next Education day will be held in Toronto, from June 5 & 6 at the Ramada Plaza on Jarvis Street. We will be having 2 full days of lectures this year. For further details check out our website at http://www.nshregionix.org/education.html Hope to see you there! Mark Elliott Region IX Education Chair ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From saby_joseph_a <@t> yahoo.com Mon Mar 30 17:22:56 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Mar 30 17:23:00 2009 Subject: [Histonet] owners manuals In-Reply-To: References: Message-ID: <758279.77976.qm@web33801.mail.mud.yahoo.com> I can personally attest that Ford must have been having a VERY bad day indeed.? He has supplied excellent information to this list on many occasions to many people over many years, and until that listing has always been very courteous. We've all had bad days.? I would suggest cutting him some slack. You can never tell who you will need, or who will come to your rescue, when the chips are down. Joe Saby ________________________________ From: Paul Verden To: histonet@lists.utsouthwestern.edu Sent: Monday, March 30, 2009 9:49:30 AM Subject: [Histonet] owners manuals Personally, I would like to thank Ford "the demon vendor" Royer at Minnesota Medical, Inc.for his extensive dissertation on the request for assistance on finding manuals.? Our lab will be expanding in the near future and will be in need of some used equipment.? It's good to know the equipment vendors you would like to avoid.? R. Paul Verden? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Mon Mar 30 20:19:22 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Mar 30 20:19:29 2009 Subject: [Histonet] owners manuals In-Reply-To: <758279.77976.qm@web33801.mail.mud.yahoo.com> References: <758279.77976.qm@web33801.mail.mud.yahoo.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C04E4FF72@mailsvr.MARSHMED.local> I couldn't agree more Joe. I have dealt with Mr. Royer on several occasions for information only and he has been very helpful. We all entitled to blow a gasket every once in a while. Be kind times are tuff. Gary (California for those that need to know) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Monday, March 30, 2009 3:23 PM To: Paul Verden; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] owners manuals I can personally attest that Ford must have been having a VERY bad day indeed.? He has supplied excellent information to this list on many occasions to many people over many years, and until that listing has always been very courteous. We've all had bad days.? I would suggest cutting him some slack. You can never tell who you will need, or who will come to your rescue, when the chips are down. Joe Saby ________________________________ From: Paul Verden To: histonet@lists.utsouthwestern.edu Sent: Monday, March 30, 2009 9:49:30 AM Subject: [Histonet] owners manuals Personally, I would like to thank Ford "the demon vendor" Royer at Minnesota Medical, Inc.for his extensive dissertation on the request for assistance on finding manuals.? Our lab will be expanding in the near future and will be in need of some used equipment.? It's good to know the equipment vendors you would like to avoid.? R. Paul Verden? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cytch7 <@t> cox.net Mon Mar 30 22:04:39 2009 From: cytch7 <@t> cox.net (Valerie Biendara) Date: Mon Mar 30 22:04:44 2009 Subject: [Histonet] Cyto prep question In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3AF3@IS-E2K3.grhs.net> Message-ID: <20090331030441.DQDI17104.eastrmmtao105.cox.net@eastrmimpo02.cox.net> This is a fairly common problem with ThinPrep slides even after educating our clinicians. We have found that vortexing the vial a minute or two just before processing helps with the cellularity. If you visually inspect the vial there is quite a bit of clumping. I think the vortexing helps break apart the clumps. The recovery rate seems to depend on how much lubricant is present in the vial. Vortexing is not 100% effective but it has cut down our unsatisfactory rate. Valerie N Biendara SCT(ASCP)IAC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, March 30, 2009 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyto prep question I know this many not be the forum to ask this question, but I know many of us have cyto depts. within our labs. We have been seeing an increase in the number of unsatisfactory urine specimens from our Urology group lately and it is due to lubricant on the ThinPrep filter. We have worked with the Urology group and they are using the recommended lubricant from the manufactory. We see the problem even when the urine is diluted in CytoLyt for several hours. Has anyone else experienced this problem? Thanks in advance, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Tue Mar 31 00:33:21 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Mar 31 00:34:14 2009 Subject: [Histonet] Calcium salts Message-ID: <49D237C0.471C.0039.0@health.qld.gov.au> Hello I am trying to differentiate Calcium Phosphate from Calcium Carbonate which both stain with the von Kossa method. Is there a pretreatment for which only one is soluble that could be used to differentiate the 2 components. thanks Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Michelle.Perrins <@t> uct.ac.za Tue Mar 31 07:06:16 2009 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Tue Mar 31 07:06:35 2009 Subject: [Histonet] opinion please Message-ID: <49D22357.A704.0070.0@uct.ac.za> Hi I would like your opinion of the manual vs semi/fully automated rotary microtome, especially with regard to stress on the hands. Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za From rjbuesa <@t> yahoo.com Tue Mar 31 08:09:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 31 08:09:18 2009 Subject: [Histonet] opinion please In-Reply-To: <49D22357.A704.0070.0@uct.ac.za> Message-ID: <89714.11192.qm@web65715.mail.ac4.yahoo.com> Michelle: The motorized microtome was introduced specifically to alleviate the carpal-tunnel syndrome quite frequent in histotechs that have been sectioning for years. It is very advantageous and can take care of the trimming necessary to reach the area that is going to be sectioned and even then it can take care of sectioning for which it has different speed modes. Another advantage is that the rhythm is aways constant and can be regulated. They are a good investment. Ren? J. --- On Tue, 3/31/09, Michelle Perrins wrote: From: Michelle Perrins Subject: [Histonet] opinion please To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 8:06 AM Hi I would like your opinion of the manual vs semi/fully automated rotary microtome, especially with regard to stress on the hands. Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerry.santiago <@t> jax.ufl.edu Tue Mar 31 08:11:24 2009 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Tue Mar 31 08:11:29 2009 Subject: [Histonet] PDGFR Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF30148F3FD@jaxmail.umc.ufl.edu> Any lab performing PDGFR (Platelet Derived Growth Factor Receptors)? Please contact me directly. Jerry.santiago@jax.ufl.edu Jerry Santiago Shands Jacksonville Jacksonville, Florida 904-244-6149 From cathy <@t> wasatchhisto.com Tue Mar 31 08:29:52 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Tue Mar 31 08:30:12 2009 Subject: [Histonet] free to good home AO 820 microtome Message-ID: I have an AO 820 microtome with gray cover free to a good. It still works great and was replaced with a new microtome we used in the lab. Has been sitting idle with a dust cover for many years. Just pay the shipping to its new home. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com From bernietaupin <@t> ymail.com Tue Mar 31 08:50:26 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Tue Mar 31 08:52:34 2009 Subject: [Histonet] Ford Royer Message-ID: <930298.3241.qm@web43515.mail.sp1.yahoo.com> > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. From vazquezr <@t> ohsu.edu Tue Mar 31 09:08:16 2009 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Mar 31 09:08:27 2009 Subject: [Histonet] Ford Royer In-Reply-To: <930298.3241.qm@web43515.mail.sp1.yahoo.com> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Mar 31 09:15:51 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Mar 31 09:16:33 2009 Subject: [Histonet] onalighternote In-Reply-To: <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Mar 31 09:18:36 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Mar 31 09:19:59 2009 Subject: [Histonet] onalighternote In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04A9@LTA3VS011.ees.hhs.gov> I wanted to ask how Elton was but thought that was a bit too easy....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Tue Mar 31 09:23:00 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Mar 31 09:23:15 2009 Subject: [Histonet] onalighternote In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com><2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> Message-ID: I think you meant to say CD. :) Have a grand day! Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debra.Ortiz <@t> uchospitals.edu Tue Mar 31 09:30:18 2009 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Tue Mar 31 09:30:28 2009 Subject: [Histonet] (no subject) Message-ID: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu> We are currently handling all prostate, lung, heart, and breast biopsies as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for H&E's and the rest as unstained plus slides. What has happened is that we throw thousands of unused expensive slides a year because they are not used. Is there a general standard in practice out there for FNBs? Unfortunately, with the economy the way it is; and trying always to find ways to bring down expenses this seems counteractive. Thank you Debi Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From Debra.Ortiz <@t> uchospitals.edu Tue Mar 31 09:43:23 2009 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Tue Mar 31 09:43:36 2009 Subject: [Histonet] (no subject) In-Reply-To: References: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu> Message-ID: <5392DB699B157E4C8E65B3779634BD7D011AF54D@uchmbx04-hpk03s.UCHAD.uchospitals.edu> Thank you for your very prompt response. I do agree that we are here for the patient. Unfotunately I am under the gun to bring down costs and I thought that might be one way. Thanks again, and look forward to other responses. Debi ________________________________ From: Lorraine Cornett [mailto:cornettl@hotmail.com] Sent: Tuesday, March 31, 2009 9:36 AM To: Ortiz, Debra [UCH] Subject: RE: [Histonet] (no subject) Yes, Unfortunately(financially) it is current practice at our facility as well. As a preemptive measure, I keep a record of the number of all unstained slides(total number only) thrown out on a monthly basis. The only alternative is to not cut the extra's and take a chance on not getting the proper area on the slide for diagnostic stains. I think that patient care is of the utmost importance, and this probably is a small price to pay in the grand scheme of things. Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Tue, 31 Mar 2009 09:30:18 -0500 > From: Debra.Ortiz@uchospitals.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > We are currently handling all prostate, lung, heart, and breast biopsies > as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for > H&E's and the rest as unstained plus slides. What has happened is that > we throw thousands of unused expensive slides a year because they are > not used. Is there a general standard in practice out there for FNBs? > Unfortunately, with the economy the way it is; and trying always to find > ways to bring down expenses this seems counteractive. > > > > Thank you > > Debi > > > > Debra Ann Ortiz > > Chief Medical Technologist > > The University of Chicago Medical Center > > Room E-602-A > > 5841 S. Maryland Avenue > > Chicago, Il 60637 > > phone: 773.702.5237 > > > > ************************************************************************ ******** > This e-mail is intended only for the use of the individual or entity to which > it is addressed and may contain information that is privileged and confidential. > If the reader of this e-mail message is not the intended recipient, you are > hereby notified that any dissemination, distribution or copying of this > communication is prohibited. If you have received this e-mail in error, please > notify the sender and destroy all copies of the transmittal. > > Thank you > University of Chicago Medical Center > ************************************************************************ ******** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Windows Live(tm) SkyDrive: Get 25 GB of free online storage. Check it out. ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From ooi.ting.huay <@t> nhc.com.sg Tue Mar 31 09:49:45 2009 From: ooi.ting.huay <@t> nhc.com.sg (ooi.ting.huay@nhc.com.sg) Date: Tue Mar 31 09:50:05 2009 Subject: [Histonet] staining of fibrin, muscle, collagen and elastic fiber Message-ID: Hi, I am interested to look at the fibrin, muscle, collagen and e lastic fiber distribution in the artery in the plastic resin section. Any s that Movat pen fiber. Do anyone know the that is available for all the stai regarding the staining for the desired stain? Regards, Ooi From HornHV <@t> archildrens.org Tue Mar 31 09:50:18 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Mar 31 09:50:22 2009 Subject: [Histonet] (no subject) In-Reply-To: <5392DB699B157E4C8E65B3779634BD7D011AF54D@uchmbx04-hpk03s.UCHAD.uchospitals.edu> References: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu> <5392DB699B157E4C8E65B3779634BD7D011AF54D@uchmbx04-hpk03s.UCHAD.uchospitals.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830ED@EMAIL.archildrens.org> I agree with Lorraine on this one. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra.Ortiz@uchospitals.edu Sent: Tuesday, March 31, 2009 9:43 AM To: cornettl@hotmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Thank you for your very prompt response. I do agree that we are here for the patient. Unfotunately I am under the gun to bring down costs and I thought that might be one way. Thanks again, and look forward to other responses. Debi ________________________________ From: Lorraine Cornett [mailto:cornettl@hotmail.com] Sent: Tuesday, March 31, 2009 9:36 AM To: Ortiz, Debra [UCH] Subject: RE: [Histonet] (no subject) Yes, Unfortunately(financially) it is current practice at our facility as well. As a preemptive measure, I keep a record of the number of all unstained slides(total number only) thrown out on a monthly basis. The only alternative is to not cut the extra's and take a chance on not getting the proper area on the slide for diagnostic stains. I think that patient care is of the utmost importance, and this probably is a small price to pay in the grand scheme of things. Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Tue, 31 Mar 2009 09:30:18 -0500 > From: Debra.Ortiz@uchospitals.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > We are currently handling all prostate, lung, heart, and breast biopsies > as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for > H&E's and the rest as unstained plus slides. What has happened is that > we throw thousands of unused expensive slides a year because they are > not used. Is there a general standard in practice out there for FNBs? > Unfortunately, with the economy the way it is; and trying always to find > ways to bring down expenses this seems counteractive. > > > > Thank you > > Debi > > > > Debra Ann Ortiz > > Chief Medical Technologist > > The University of Chicago Medical Center > > Room E-602-A > > 5841 S. Maryland Avenue > > Chicago, Il 60637 > > phone: 773.702.5237 > > > > ************************************************************************ ******** > This e-mail is intended only for the use of the individual or entity to which > it is addressed and may contain information that is privileged and confidential. > If the reader of this e-mail message is not the intended recipient, you are > hereby notified that any dissemination, distribution or copying of this > communication is prohibited. If you have received this e-mail in error, please > notify the sender and destroy all copies of the transmittal. > > Thank you > University of Chicago Medical Center > ************************************************************************ ******** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Windows Live(tm) SkyDrive: Get 25 GB of free online storage. Check it out. ************************************************************************ ******** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ************************************************************************ ******** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tpodawiltz <@t> lrgh.org Tue Mar 31 10:12:24 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Mar 31 10:13:58 2009 Subject: [Histonet] (no subject) In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830ED@EMAIL.archildrens.org> References: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu> <5392DB699B157E4C8E65B3779634BD7D011AF54D@uchmbx04-hpk03s.UCHAD.uchospitals.edu>, <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830ED@EMAIL.archildrens.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D33B5BD35@LRGHEXVS1.practice.lrgh.org> We do not throw them out, we file them with the stained slides for future reference. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV@archildrens.org] Sent: Tuesday, March 31, 2009 10:50 AM To: Debra.Ortiz@uchospitals.edu; cornettl@hotmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) I agree with Lorraine on this one. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra.Ortiz@uchospitals.edu Sent: Tuesday, March 31, 2009 9:43 AM To: cornettl@hotmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Thank you for your very prompt response. I do agree that we are here for the patient. Unfotunately I am under the gun to bring down costs and I thought that might be one way. Thanks again, and look forward to other responses. Debi ________________________________ From: Lorraine Cornett [mailto:cornettl@hotmail.com] Sent: Tuesday, March 31, 2009 9:36 AM To: Ortiz, Debra [UCH] Subject: RE: [Histonet] (no subject) Yes, Unfortunately(financially) it is current practice at our facility as well. As a preemptive measure, I keep a record of the number of all unstained slides(total number only) thrown out on a monthly basis. The only alternative is to not cut the extra's and take a chance on not getting the proper area on the slide for diagnostic stains. I think that patient care is of the utmost importance, and this probably is a small price to pay in the grand scheme of things. Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Tue, 31 Mar 2009 09:30:18 -0500 > From: Debra.Ortiz@uchospitals.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > We are currently handling all prostate, lung, heart, and breast biopsies > as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for > H&E's and the rest as unstained plus slides. What has happened is that > we throw thousands of unused expensive slides a year because they are > not used. Is there a general standard in practice out there for FNBs? > Unfortunately, with the economy the way it is; and trying always to find > ways to bring down expenses this seems counteractive. > > > > Thank you > > Debi > > > > Debra Ann Ortiz > > Chief Medical Technologist > > The University of Chicago Medical Center > > Room E-602-A > > 5841 S. Maryland Avenue > > Chicago, Il 60637 > > phone: 773.702.5237 > > > > ************************************************************************ ******** > This e-mail is intended only for the use of the individual or entity to which > it is addressed and may contain information that is privileged and confidential. > If the reader of this e-mail message is not the intended recipient, you are > hereby notified that any dissemination, distribution or copying of this > communication is prohibited. If you have received this e-mail in error, please > notify the sender and destroy all copies of the transmittal. > > Thank you > University of Chicago Medical Center > ************************************************************************ ******** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Windows Live(tm) SkyDrive: Get 25 GB of free online storage. Check it out. ************************************************************************ ******** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ************************************************************************ ******** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Vickroy.Jim <@t> mhsil.com Tue Mar 31 10:45:20 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 31 10:46:35 2009 Subject: [Histonet] microwave tissue processor Message-ID: <24A4826E8EF0964D86BC5317306F58A52BB25933FC@mmc-mail.ad.mhsil.com> We are thinking about Microwave Tissue processors. Unfortunately I have been given very little time to present a budget request. Can anyone comment on a recent purchase of a microwave tissue processor and their experiences? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From cmiller <@t> gladstone.ucsf.edu Tue Mar 31 10:46:21 2009 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Tue Mar 31 10:47:14 2009 Subject: [Histonet] free to good home AO 820 microtome In-Reply-To: References: Message-ID: Yes please!!! I am desperate for a new microtome!!! Caroline Caroline Miller M.Sc DIC AIBMS Co-manager Gladstone Microscopy and Histology Core J David Gladstone institute 1650 Owens Street San Francisco CA 94158 www.gladstone.ucsf.edu On Mar 31, 2009, at 6:29, "Cathy Mayton" wrote: > I have an AO 820 microtome with gray cover free to a good. It still > works great and was replaced with a new microtome we used in the > lab. Has been sitting idle with a dust cover for many years. Just > pay the shipping to its new home. > > > Cathy A. Mayton > Wasatch Histo Consultants, Inc. > 80 Youngberg Road > Winnemucca, NV 89445 > 775-625-4425 > www.wasatchhisto.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Mar 31 11:43:41 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Mar 31 11:43:46 2009 Subject: [Histonet] microwave tissue processor In-Reply-To: <24A4826E8EF0964D86BC5317306F58A52BB25933FC@mmc-mail.ad.mhsil.com> Message-ID: <5C2DCD9775C64799BE4D631A9EA8A775@wchsys.org> Milestone makes excellent microwave processors for small GI biopsies and for large tissues. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, March 31, 2009 11:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] microwave tissue processor We are thinking about Microwave Tissue processors. Unfortunately I have been given very little time to present a budget request. Can anyone comment on a recent purchase of a microwave tissue processor and their experiences? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From bernietaupin <@t> ymail.com Tue Mar 31 11:52:51 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Tue Mar 31 11:52:55 2009 Subject: [Histonet] onalighternote In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04A9@LTA3VS011.ees.hhs.gov> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> <9A16CB5D55FC1648ADF11B63E72A1BE1BF04A9@LTA3VS011.ees.hhs.gov> Message-ID: <36622.52996.qm@web43504.mail.sp1.yahoo.com> Yeah, hilarious. I swear, I haven't heart THAT one before ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Edwards, R.E." ; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:18:36 AM Subject: RE: [Histonet] onalighternote I wanted to ask how Elton was but thought that was a bit too easy....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Mar 31 11:48:23 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Mar 31 11:53:45 2009 Subject: [Histonet] microwave tissue processor In-Reply-To: <24A4826E8EF0964D86BC5317306F58A52BB25933FC@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A52BB25933FC@mmc-mail.ad.mhsil.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04B2@LTA3VS011.ees.hhs.gov> We have had great success with our Sakura Xpress. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, March 31, 2009 11:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] microwave tissue processor We are thinking about Microwave Tissue processors. Unfortunately I have been given very little time to present a budget request. Can anyone comment on a recent purchase of a microwave tissue processor and their experiences? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dwaugh <@t> kent.edu Tue Mar 31 12:33:41 2009 From: dwaugh <@t> kent.edu (David Waugh) Date: Tue Mar 31 12:33:51 2009 Subject: [Histonet] AO disposable blade holder Message-ID: <25107FC7-3279-4C4E-840B-7B2470DDBBD5@kent.edu> I have a question regarding a AO disposable blade holder (Cat# 824). This link should show a picture of a similar one (http:// www.usedmicroscopes.com/images/AO820Microtome.jpg) The blade is tightened with a lever on the right that rotates a d- shaped cam. Two screws are used the set the tension that the cam places on the blade, once set, the cam will tighten and loosen the blade. The back of the holder is concave, and the front part convex, so that when tightened, the blade will bow. I have not been able to find any documentation on how tight the blade should be. It sounds like most holders have flat surfaces that clamp the blade, which I would think would have very different tightness requirements. Any ideas from someone who uses this type of holder on how the two screws should be set? Thanks, David Kent State University Department of Geology Kent, Ohio 44242 dwaugh@kent.edu www.personal.kent.edu/~dwaugh From tbraud <@t> holyredeemer.com Tue Mar 31 12:34:35 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Mar 31 12:34:41 2009 Subject: [Histonet] RE: cutting protocols with extra slides In-Reply-To: <3f793e550001b425@HolyRedeemer.com> Message-ID: Hi Debi - I've seen protocols similar to what you describe, and in my experience, they are almost always a result of someone, at some point in time, cutting through (wasting) a critical small specimen after the initial H&Es were cut or during multiple levels. Perhaps the case can be made that you can insure that it will not happen? Maybe offer to separate the protocols by tissue type...for example, try to reduce or eliminate extra slides for less critical or larger needle biopsies such as prostate, breast, etc., and keep it for tiny or difficult specimens such as cardiac biopsies, In some labs, even 3 H&E's would be considered excessive. Best of luck to you in your quest. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax Message: 17 Date: Tue, 31 Mar 2009 09:30:18 -0500 From: We are currently handling all prostate, lung, heart, and breast biopsies as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for H&E's and the rest as unstained plus slides. What has happened is that we throw thousands of unused expensive slides a year because they are not used. Is there a general standard in practice out there for FNBs? Unfortunately, with the economy the way it is; and trying always to find ways to bring down expenses this seems counteractive. Thank you Debi Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From dwaugh <@t> kent.edu Tue Mar 31 12:43:23 2009 From: dwaugh <@t> kent.edu (David Waugh) Date: Tue Mar 31 12:43:37 2009 Subject: [Histonet] AO disposable blade holder Message-ID: I have a question regarding a AO disposable blade holder (Cat# 824). This link should show a picture of a similar one (http:// www.usedmicroscopes.com/images/AO820Microtome.jpg) The blade is tightened with a lever on the right that rotates a d- shaped cam. Two screws are used the set the tension that the cam places on the blade, once set, the cam will tighten and loosen the blade. The back of the holder is concave, and the front part convex, so that when tightened, the blade will bow. I have not been able to find any documentation on how tight the blade should be. It sounds like most holders have flat surfaces that clamp the blade, which I would think would have very different tightness requirements. Any ideas from someone who uses this type of holder on how the two screws should be set? Thanks, David Kent State University Department of Geology Kent, Ohio 44242 dwaugh@kent.edu www.personal.kent.edu/~dwaugh From dwaugh <@t> kent.edu Tue Mar 31 13:16:44 2009 From: dwaugh <@t> kent.edu (David Waugh) Date: Tue Mar 31 13:16:55 2009 Subject: [Histonet] AO Blade holder Message-ID: I have a question regarding a AO disposable blade holder (Cat# 824). This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg The blade is tightened with a lever on the right that rotates a d- shaped cam. Two screws are used the set the tension that the cam places on the blade, once set, the cam will tighten and loosen the blade. The back of the holder is concave, and the front part convex, so that when tightened, the blade will bow. I have not been able to find any documentation on how tight the blade should be. It sounds like most holders have flat surfaces that clamp the blade, which I would think would have very different tightness requirements. Any ideas from someone who uses this type of holder on how the two screws should be set? Thanks, David Kent State University Department of Geology Kent, Ohio 44242 dwaugh@kent.edu From Bauer.Karen <@t> mayo.edu Tue Mar 31 13:29:19 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Mar 31 13:29:47 2009 Subject: [Histonet] Online SOP's Message-ID: <3EEADAB0392281448505732B7323D9A4047676@MSGEBE37.mfad.mfroot.org> Hello, Does anyone have only procedures online? All of ours are online, but we still keep a paper copy of each in a manual in the lab. I would like to get rid of the manual, but then how do you record pathologist sign-off on all new or revised procedures? I'm curious to hear what other labs are doing. Thanks much, Karen Karen L. Bauer HT(ASCP), BS Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu From dwaugh <@t> kent.edu Tue Mar 31 14:08:08 2009 From: dwaugh <@t> kent.edu (David Waugh) Date: Tue Mar 31 14:08:15 2009 Subject: [Histonet] AO Blade holder In-Reply-To: References: Message-ID: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> > I have a question regarding a AO disposable blade holder (Cat# 824). > This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg > > The blade is tightened with a lever on the right that rotates a d- > shaped cam. Two screws are used the set the tension that the cam > places on the blade, once set, the cam will tighten and loosen the > blade. The back of the holder is concave, and the front part convex, > so that when tightened, the blade will bow. I have not been able to > find any documentation on how tight the blade should be. It sounds > like most holders have flat surfaces that clamp the blade, which I > would think would have very different tightness requirements. Any > ideas from someone who uses this type of holder on how the two > screws should be set? Thanks, David > > Kent State University > Department of Geology > Kent, Ohio 44242 > dwaugh@kent.edu > From aeshnidae <@t> hotmail.com Tue Mar 31 14:25:50 2009 From: aeshnidae <@t> hotmail.com (Leslie McCormack) Date: Tue Mar 31 14:25:55 2009 Subject: [Histonet] Effects of Cytocool on IHC In-Reply-To: References: Message-ID: In our lab many of the histotechs use Cytocool when cutting paraffin blocks. Has anyone heard of this having a negative effect on IHC? Leslie McCormack Baptist Medical Center Jacksonville, FL 32207 _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail?. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_WL_HM_express_032009#colortheme From SwainFrancesL <@t> uams.edu Tue Mar 31 14:25:45 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Tue Mar 31 14:26:26 2009 Subject: [Histonet] AO Blade holder In-Reply-To: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> References: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> Message-ID: Hi David: When I used an AO with a disposable blade holder. I had to test a blank paraffin block to get the blade the right tightness. There was no set tension that I can remember. What I would do it tighten the blade holder until the disposable blade was held securely. Test the knife with the blank block. If the blade was too tight I would loosen the lever and back the screws off 1/4 turn. Tighten it back up and test it again. I would continue doing this until I got the right tension. It really sounds complicated but it really isn't just make sure that the springs are good and the lever is clean. Once you get it set you are good to go until you clean your holder and then you have to go through the procedure again. This is the best that I can remember. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Waugh Sent: Tuesday, March 31, 2009 2:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AO Blade holder > I have a question regarding a AO disposable blade holder (Cat# 824). > This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg > > The blade is tightened with a lever on the right that rotates a d- > shaped cam. Two screws are used the set the tension that the cam > places on the blade, once set, the cam will tighten and loosen the > blade. The back of the holder is concave, and the front part convex, > so that when tightened, the blade will bow. I have not been able to > find any documentation on how tight the blade should be. It sounds > like most holders have flat surfaces that clamp the blade, which I > would think would have very different tightness requirements. Any > ideas from someone who uses this type of holder on how the two > screws should be set? Thanks, David > > Kent State University > Department of Geology > Kent, Ohio 44242 > dwaugh@kent.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Tue Mar 31 14:32:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 31 14:32:50 2009 Subject: [Histonet] AO Blade holder In-Reply-To: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> Message-ID: <407558.12673.qm@web65703.mail.ac4.yahoo.com> With the lever loose, tight the two screws in a way that the blade is free and can be moved in any direction. The lever will lock the blade and it should be tighten "all the way". Ren? J. --- On Tue, 3/31/09, David Waugh wrote: From: David Waugh Subject: [Histonet] AO Blade holder To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 3:08 PM > I have a question regarding a AO disposable blade holder (Cat# 824). This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg > > The blade is tightened with a lever on the right that rotates a d-shaped cam. Two screws are used the set the tension that the cam places on the blade, once set, the cam will tighten and loosen the blade. The back of the holder is concave, and the front part convex, so that when tightened, the blade will bow. I have not been able to find any documentation on how tight the blade should be. It sounds like most holders have flat surfaces that clamp the blade, which I would think would have very different tightness requirements. Any ideas from someone who uses this type of holder on how the two screws should be set? Thanks, David > > Kent State University > Department of Geology > Kent, Ohio 44242 > dwaugh@kent.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly.Poole <@t> drdc-rddc.gc.ca Tue Mar 31 14:38:34 2009 From: Kimberly.Poole <@t> drdc-rddc.gc.ca (Poole, Kimberly) Date: Tue Mar 31 14:38:44 2009 Subject: [Histonet] Histology Training Message-ID: <42DFE1A029181B4B8CCBA7261B52D765EA51DC@suffieldex01.suffield.drdc-rddc.gc.ca> Hi, Does anyone know of a place that I can get histology training? I am specifically looking for a place in the US or Canada to go to for a couple of weeks. I am not able to take courses that are 1 to 2 years long. Thanks in advance!! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From Rcartun <@t> harthosp.org Tue Mar 31 14:45:20 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 31 14:45:32 2009 Subject: [Histonet] (no subject) In-Reply-To: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu> References: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu> Message-ID: <49D23A90020000770000A8E8@gwmail4.harthosp.org> Here at Hartford Hospital we do the following for biopsies: Prostate: 5 slides (1, 3, and 5 - H&E/2 and 4 - unstain) Lung: 5 slides (1, 3, and 5 - H&E/2 and 4 - unstain) Breast: 7 slides (1, 4, and 7 - H&E/ 2 ,3, 5, and 6 - unstain) Transplant heart: 8 slides (1, 3, 5, and 7 - H&E/2, 4, 6, and 8 - unstain) Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 3/31/2009 10:30 AM >>> We are currently handling all prostate, lung, heart, and breast biopsies as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for H&E's and the rest as unstained plus slides. What has happened is that we throw thousands of unused expensive slides a year because they are not used. Is there a general standard in practice out there for FNBs? Unfortunately, with the economy the way it is; and trying always to find ways to bring down expenses this seems counteractive. Thank you Debi Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Mar 31 14:55:17 2009 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Tue Mar 31 14:57:18 2009 Subject: [Histonet] (no subject) In-Reply-To: <49D23A90020000770000A8E8@gwmail4.harthosp.org> References: <5392DB699B157E4C8E65B3779634BD7D011AF54C@uchmbx04-hpk03s.UCHAD.uchospitals.edu>, <49D23A90020000770000A8E8@gwmail4.harthosp.org> Message-ID: We file all our blank slides with the stained slides. That way we can always go back to them rather than cutting any more on an already minute piece of tissue remaining. Also, for legal reasons, what if the blanks that are discarded contained tumor? (or NO tumor) IE: Showing something other than what the original stained slides showed. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: March 31, 2009 1:45 PM To: histonet@lists.utsouthwestern.edu; Debra.Ortiz@uchospitals.edu Subject: Re: [Histonet] (no subject) Here at Hartford Hospital we do the following for biopsies: Prostate: 5 slides (1, 3, and 5 - H&E/2 and 4 - unstain) Lung: 5 slides (1, 3, and 5 - H&E/2 and 4 - unstain) Breast: 7 slides (1, 4, and 7 - H&E/ 2 ,3, 5, and 6 - unstain) Transplant heart: 8 slides (1, 3, 5, and 7 - H&E/2, 4, 6, and 8 - unstain) Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 3/31/2009 10:30 AM >>> We are currently handling all prostate, lung, heart, and breast biopsies as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for H&E's and the rest as unstained plus slides. What has happened is that we throw thousands of unused expensive slides a year because they are not used. Is there a general standard in practice out there for FNBs? Unfortunately, with the economy the way it is; and trying always to find ways to bring down expenses this seems counteractive. Thank you Debi Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From mbisher <@t> Princeton.EDU Tue Mar 31 15:05:47 2009 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Tue Mar 31 15:05:52 2009 Subject: [Histonet] Problems with Cutting In-Reply-To: <49D23A90020000770000A8E8@gwmail4.harthosp.org> Message-ID: Hi Histonetters, I have someone here who is having problems with their samples (mouse lung and mammary). It appears that the sample is very brittle when she cuts and in addition, some of the paraffin around the sample in the cassette is very white in color instead of the normal translucent color. I think it is poor infiltration of some kind. I told her about this great resource, this website, and I told her I would get lots of helpful responses. If anyone would like a picture, I could send you one. Thank you, Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu From wdesalvo.cac <@t> hotmail.com Tue Mar 31 15:10:07 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Mar 31 15:10:13 2009 Subject: [Histonet] Looking for Lab Message-ID: I have heard that there is a research lab in Baton Rouge, LA that specialises in Leprosy cases. I have a request from one of my Derm Paths to locate so that we can send a difficult case. I would apprciate any information. Thanks William DeSalvo, B.S. HTL(ASCP) Production Manager Sonora Quest Laboratory _________________________________________________________________ Windows Live? SkyDrive: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009 From rjbuesa <@t> yahoo.com Tue Mar 31 15:41:36 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 31 15:41:43 2009 Subject: [Histonet] Looking for Lab In-Reply-To: Message-ID: <220167.72042.qm@web65709.mail.ac4.yahoo.com> Bill: I think you refer to the National Hansen's Disease Center at the Louisiana State?University in Baton Rouge. If you contact the LSU for sure they can tell you about it. Ren? J. --- On Tue, 3/31/09, WILLIAM DESALVO wrote: From: WILLIAM DESALVO Subject: [Histonet] Looking for Lab To: "histonet" Date: Tuesday, March 31, 2009, 4:10 PM I have heard that there is a research lab in Baton Rouge, LA that specialises in Leprosy cases. I have a request from one of my Derm Paths to locate so that we can send a difficult case. I would apprciate any information. Thanks William DeSalvo, B.S. HTL(ASCP) Production Manager Sonora Quest Laboratory _________________________________________________________________ Windows Live? SkyDrive: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juditw <@t> u.washington.edu Tue Mar 31 15:41:48 2009 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Tue Mar 31 15:41:52 2009 Subject: [Histonet] Looking for Lab In-Reply-To: Message-ID: Hi - I think it is the Pennington Labs in BR. If you can contact Cheryl Crowder in BR at ccrowder@vetmed.lsu.edu she will probably know for sure. Judy in Washington (A tiger grad- geaux tigers!) On Tue, 31 Mar 2009, WILLIAM DESALVO wrote: > > I have heard that there is a research lab in Baton Rouge, LA that specialises in Leprosy cases. I have a request from one of my Derm Paths to locate so that we can send a difficult case. I would apprciate any information. Thanks > > > > William DeSalvo, B.S. HTL(ASCP) > > Production Manager > > Sonora Quest Laboratory > > _________________________________________________________________ > Windows Live? SkyDrive: Get 25 GB of free online storage. > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_032009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From LSebree <@t> uwhealth.org Tue Mar 31 16:04:10 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 31 16:04:15 2009 Subject: [Histonet] Effects of Cytocool on IHC In-Reply-To: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF3F@UWHC-MAIL01.uwhis.hosp.wisc.edu> I have heard not to use freezing spray on blocks/sections that are destined for IHC but I never heard the reasoning behind this warning. I've always steered clear of it when cutting for IHC and instead will bury my block in the frost of our freezer until its good and cold. Works fine. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leslie McCormack Sent: Tuesday, March 31, 2009 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Effects of Cytocool on IHC In our lab many of the histotechs use Cytocool when cutting paraffin blocks. Has anyone heard of this having a negative effect on IHC? Leslie McCormack Baptist Medical Center Jacksonville, FL 32207 _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail(r). http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_M SGTX_WL_HM_express_032009#colortheme____________________________________ ___________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.PALMER <@t> svhm.org.au Tue Mar 31 17:26:59 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Tue Mar 31 17:27:06 2009 Subject: [Histonet] Re: IHC automated buffers References: Message-ID: We also make our own TBS for our Dako autostainer, same as Jan. I have used PBS instead of TBS at times, when transfering a bench run where I used PBS / tween (my standard buffer) to the machine. I wonder if TBS really does give better results than TBS, when averaged out over all Abs? I do know that TBS gets fungal contamination more easily than PBS (due to the organic / oxidisable nature of tris) and therefore has to be replaced more frequently, which is a drag! Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Date: Mon, 30 Mar 2009 12:48:38 -0500 From: "Jan Shivers" Subject: Re: [Histonet] IHC automated buffers To: "Charles, Roger" , Message-ID: <13EC8A4018C84A82A43104D961F1C2B1@auxs.umn.edu> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original We mix up our own, using Tween 20 at 0.05% per volume. No problems here, when using a Dako autostainer. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Charles, Roger" To: Sent: Monday, March 30, 2009 9:53 AM Subject: [Histonet] IHC automated buffers Hello, Is anyone presently making their own TBS with tween 20 buffer to be used in automated IHC? I would like to know if there are any problems related to these buffers and if any deviations must be made to accommodate the automation. Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From AnthonyH <@t> chw.edu.au Tue Mar 31 17:27:29 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 31 17:27:44 2009 Subject: [Histonet] Calcium salts In-Reply-To: <49D237C0.471C.0039.0@health.qld.gov.au> Message-ID: Tony, I have been lead to believe that after von Kossa staining, Silver carbonate dissolves in sodium thiosulphate and cannot be demonstrated with von Kossa's technique. Though I have not confirmed this Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Tuesday, 31 March 2009 4:33 PM To: histonet Subject: [Histonet] Calcium salts Hello I am trying to differentiate Calcium Phosphate from Calcium Carbonate which both stain with the von Kossa method. Is there a pretreatment for which only one is soluble that could be used to differentiate the 2 components. thanks Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au ************************************************************************ ******** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ************************************************************************ ********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From vapatpxs <@t> yahoo.com Tue Mar 31 18:01:14 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Tue Mar 31 18:01:17 2009 Subject: [Histonet] onalighternote Message-ID: <408230.81711.qm@web46106.mail.sp1.yahoo.com> Hey, we can't help it if your parents were big fans of his. He is a great song writer. ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 3/31/09, Bernie Taupin wrote: > From: Bernie Taupin > Subject: Re: [Histonet] onalighternote > To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." , histonet@lists.utsouthwestern.edu > Date: Tuesday, March 31, 2009, 4:52 PM > Yeah, hilarious.. I swear, I haven't > heart THAT one before > > > > > ________________________________ > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Edwards, R.E." ; > histonet@lists.utsouthwestern.edu > Sent: Tuesday, March 31, 2009 10:18:36 AM > Subject: RE: [Histonet] onalighternote > > I wanted to ask how Elton was but thought that was a bit > too easy....... > > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Edwards, > R.E. > Sent: Tuesday, March 31, 2009 10:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] onalighternote > > > I have? just? had? eeees from? Bernie > Taupin and Paul Schofield, is > this? a? record??. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Robyn > Vazquez > Sent: 31 March 2009 15:08 > To: Bernie Taupin; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ford Royer > > I think Ford gets the message...GET ON WITH IT!? > Antibody talk anyone? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Bernie > Taupin > Sent: Tuesday, March 31, 2009 6:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ford Royer > > > I can personally attest that Ford must have been > having a VERY bad day > indeed. > > Although I can empathize that this might be the case, I do > find it > curious that we've heard nothing from Mr. Royer since his > little > outburst. > > I, for one, wouldn't mind an apology to the list for your > antisocial > behaviour on it, Mr. Royer. > > > > ? ? ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ? ? ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From macveigh <@t> usc.edu Tue Mar 31 19:39:01 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Mar 31 19:38:35 2009 Subject: [Histonet] Leica Bond Max - any opinions? Message-ID: <001101c9b262$40984570$5c237d80@DFS66DD1> Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA From winston <@t> saclink.csus.edu Tue Mar 31 13:41:06 2009 From: winston <@t> saclink.csus.edu (Lancaster, Winston C) Date: Thu Apr 2 09:58:53 2009 Subject: [Histonet] skin sectioning Message-ID: <813DAC550C738E4BBFF73EBFD204DC2C11D8A111B9@VSL4.saclink.csus.edu> Greetings, I have been cutting sections of whole bat genitalia embedded in JB-4. My intention is to study and photograph these sections in light microscopy. The quality of the sections is excellent except for one detail. The medium separates from the epidermis (but not any other tissue interface). At first I thought it was the hair, but the separation occurred even if I trimmed the hairs. I also tried to section skin from the bat's torso and had similar results. I have left the specimens to impregnate literally for days. The bat's penis is about 4mm long and 1mm in diameter at the base; female parts are slightly wider and shorter. My lab is set up for sectioning with glass knives and I wanted to avoid the shrinkage of paraffin. Can anyone suggest a method to avoid this problem with the JB-4, or another medium? Winston C. Lancaster Dept. of Biological Sciences California State University, Sacramento Sacramento, California 95819 6077 wlancaster@csus.edu