[Histonet] Re: Histonet Digest, Vol 67, Issue 32

[Nick and Amanda]

Re Message 5

If you find pariffin catchers can I please have the details too? THANX

A. Bowden
amanda.bowden <@t> ccdhb.org.nz








----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, June 30, 2009 5:02 AM
Subject: Histonet Digest, Vol 67, Issue 32


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> Today's Topics:
>
>   1. Re: Beta-Amyloid (Richard Cartun)
>   2. Millipore MAB1281 anti human nuclei IHC on FFPE
>      (PALMER Jason (SVHM))
>   3. Re: Frozen Section TAT (Phyllis Thaxton)
>   4. Signs of good perfusion (Thach, Dzung (NIH/NIAID) [E])
>   5. Cardboard Paraffin Catchers (Phyllis Thaxton)
>   6. Re: Signs of good perfusion (Merced M Leiker)
>   7. WI/MI (Steven Coakley)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 28 Jun 2009 20:47:27 -0400
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: Re: [Histonet] Beta-Amyloid
> To: "Histonet" <histonet <@t> pathology.swmed.edu>, "Sebree Linda A"
> <LSebree <@t> uwhealth.org>
> Message-ID: <4A47D6DF.7400.0077.1 <@t> harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> We've been using a monoclonal antibody (clone 6E10) from Signet 
> Laboratories (now Invitrogen) for years with excellent results.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>>>> "Sebree Linda A" <LSebree <@t> uwhealth.org> 6/24/2009 2:49 PM >>>
> Hi,
> Wondering if anyone knows of a Beta-Amyloid antibody, for use on FFPE
> brain sections, that doesn't need formic acid pretreatment?
>
> Thanks,
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
>
>
> _______________________________________________
> Histonet mailing list
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>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 29 Jun 2009 16:36:12 +1000
> From: "PALMER Jason (SVHM)" <Jason.PALMER <@t> svhm.org.au>
> Subject: [Histonet] Millipore MAB1281 anti human nuclei IHC on FFPE
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EEE122D54674BF4CA53C0D05F9BB939E04D5674D <@t> SVHM-EXCHCCR0.svhm.schs.org.au>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all.
>
> Just wondering if anyone has managed to use Millipore mouse anti human 
> nuclei antibody (clone 235-1, MAB1281) successfully with immunostaining of 
> FFPE tissues??  The datasheet says it is possible, at 1:20 and with 
> citrate retrieval but I have had no luck with this on a variety of human 
> tissues, using a standard ABC with DAB protocol. Also no luck with 
> proteinase K.  (There is also an IHC world protocol which uses 1:20 with 
> citrate.)  I see some apparent labelling of epidermal nuclei, for example, 
> maybe half of all nuclei , but also see the same thing in diluent-only 
> negative controls, so clearly the staining seen is not specific.
>
> Thanks for any help,
>
> Jason
>
> Jason Palmer
> Histology Laboratory Coordinator
> Bernard O'Brien Institute
> 42 Fitzroy St, Fitzroy Victoria 3065
> Australia
> tel +61 3 9288 4018
> fax +61 3 9416 0926
> email: jason.palmer <@t> svhm.org.au
>
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>
> ------------------------------
>
> Message: 3
> Date: Mon, 29 Jun 2009 06:58:21 -0700 (PDT)
> From: Phyllis Thaxton <dchihc <@t> yahoo.com>
> Subject: Re: [Histonet] Frozen Section TAT
> To: "Lott, Robert" <Robert.Lott <@t> trinitymedicalonline.com>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <327546.90788.qm <@t> web43502.mail.sp1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi All,
> I haven't been on in a couple of weeks, but thank all of you for the 
> discussion on FS turnaround time reporting. I thought we had everything in 
> place, but one thing we didn't even consider is when breast biopsies are 
> send for radiology before arriving for FS.
> We are also reporting receiving time as order time because they come in a 
> pneumatic zip tube in -60 seconds. I guess if there is more than one room 
> trying to send and are in line waiting to be zipped, then the order time 
> would be inaccurate the way we are reporting it.
> Thanks all!!
> Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
>
> ________________________________
> From: "Lott, Robert" <Robert.Lott <@t> trinitymedicalonline.com>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Friday, June 26, 2009 11:00:27 AM
> Subject: [Histonet] Frozen Section TAT
>
> Becky Garrison makes some very valid and interesting points below....
>
>
>
> First of all, the point about what a "critical test" is, as defined by
> JCHAO.
>
> She is correct, in that these are tests defined by the individual
> institution in policy, but not by JCAHO.
>
>
>
> Second, the item (ANP.11820) on the CAP checklist concerning turnaround
> time of intraoperative frozen sections reads as follows:
>
> The institution must "periodically" evaluate TAT and document reasons
> for delay BUT only if 90% of FS are not completed within 20 minutes.
>
>
>
> ANP.11820 Phase 1
>
>
>
> Does the laboratory periodically evaluate turnaround time for
> intraoperative frozen sections?
>
>
>
> NOTE: If 90% of frozen sections are not completed within 20 minutes,
> the laboratory must document evaluation
>
> of the reason(s) for the delay. This turnaround time is intended to
> apply to the typical single frozen section.
>
> In cases where there are multiple sequential frozen sections required on
> a single specimen (e.g., resection margins),
>
> or in cases where additional studies such as radiographic correlation
> are required, longer turnaround times may be expected.
>
>
>
> COMMENTARY:
>
>
>
> N/A
>
>
>
> REFERENCE:
>
> Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section
> turnaround time.
>
> A College of American Pathologists Q-Probes study of 32,868 frozen
> sections in 700 hospitals.
>
> Arch Pathol Lab Med. 1997;121:559-567.
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology
>
> Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL
> 35213/ 205-592-5387
>
>
>
>
>
> Message: 19
>
> Date: Thu, 25 Jun 2009 10:49:43 -0400
>
> From: "Garrison, Becky" <becky.garrison <@t> jax.ufl.edu>
>
> Subject: RE: [Histonet] Re: tracking turnaround time of
>
> intraoperativeconsultations
>
> To: "Della Speranza, Vinnie" <dellav <@t> musc.edu>, "Robert Richmond"
>
> <rsrichmond <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
>
> Message-ID:
>
> <B3CA1BAF6C5E4541867E4E79AD2412E003C2910F <@t> jaxmail.umc.ufl.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> Sorry I did not respond yesterday. The reason we just started measuring
>
> order to sign out for frozens was also prompted by preparation for our
>
> next JCAHO inspection. However, there is this distinction. The Joint
>
> Commission does not define a frozen as a critical test. The designation
>
> of critical test is left up to the individual institution. However,
>
> once your institution defines the frozen as a critical test, (indicated
>
> somewhere in a policy), you must conform to JCAHO guidelines for
>
> critical tests.
>
> And apparently this is the buzz with JCAHO watchers right now.
>
>
>
> Here we designate the IntraOp consultations for frozens (not gross only
>
> or Touch prep) and IntraOp PTH (Clinical test) as a critical tests
>
> and have started tracking order to sign out. Order time is the time the
>
> surgeon indicates 'send this to pathology' not when pathology receives
>
> the specimen.
>
>
>
> We are somewhat in uncharted waters as there is no national standard
>
> that defines target time from order to sign out. We set a 40 minute
>
> time (20-OR to Path and 20-path to completed frozen). I campaigned
>
> against a 30 minute total time (15 each) because we do have some frozens
>
> that do take over 15 minutes and this was an absolute value (unlike the
>
> CAP goal of 90% within 20 minutes). Our approach is to monitor,
>
> evaluate the data we retrieve. There will certainly be adjustments made
>
> to target time and how and what we monitor.
>
>
>
> The data collection raises more questions: how do you come up with
>
> meaningful data for multiple specimens on a single case; multiple
>
> frozens (different patients) received together or before we are finished
>
> with the first patient's frozen). This is one of those ideas that
>
> sounds good in theory but presents some challenges in execution. But it
>
> is a valid process to monitor as we periodically have surgeons complain
>
> of the time they are
>
> waiting for frozen results. This is really a joint quality management
>
> review which involves multiple departments (OR and Pathology) and how we
>
> make it better for the patient.
>
>
>
>
>
> Becky Garrison
>
> Pathology Supervisor
>
> Shands Jacksonville
>
> Jacksonville, FL 32209
>
> 904-244-6237
>
>
>
>
>
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>
> ------------------------------
>
> Message: 4
> Date: Mon, 29 Jun 2009 11:31:25 -0400
> From: "Thach, Dzung (NIH/NIAID) [E]" <thachdc <@t> niaid.nih.gov>
> Subject: [Histonet] Signs of good perfusion
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <C66E568D.D7D%thachdc <@t> niaid.nih.gov>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Everyone!!
>
>   I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
> nicking the upper right atrium of heart to collect the gushed out blood 
> and
> then perfusing through ventricle using a 21G butterfly needle and
> peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes 
> out
> of mouth.  Occasionally, I see the liver fade to light pink.  Most of the
> time the paws become white.  But the brain and spinal cord (my tissues of
> interest) are always white, and seem to have been perfused.  I was 
> wondering
> how to improve this to get more consistent good perfusions, and what signs
> should I look for to indicate good perfusion?
>
> Thanks much,
>
> Dzung
> NIAID
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT)
> From: Phyllis Thaxton <dchihc <@t> yahoo.com>
> Subject: [Histonet] Cardboard Paraffin Catchers
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <372922.65853.qm <@t> web43506.mail.sp1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Does anyone remember the little cardboard microtome trays for paraffin 
> waste? If so please email me the company that makes them.
> Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 29 Jun 2009 12:07:04 -0400
> From: Merced M Leiker <leiker <@t> buffalo.edu>
> Subject: Re: [Histonet] Signs of good perfusion
> To: "Thach, Dzung (NIH/NIAID) [E]" <thachdc <@t> niaid.nih.gov>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <1D8E958050ABAD54BD852005 <@t> CDYwxp1931.ad.med.buffalo.edu>
> Content-Type: text/plain; charset=us-ascii; format=flowed
>
> Live going pale is a good sign, your tissues of interest going pale is an
> even better sign, but fluid coming out of the mouth (or even the nose, or
> additionally, any kind of bloating or swelling in the animal) is a bad
> sign. You may not be able to get good perfusion (pale tissues) if this
> happens before your tissues turn pale, as the pressure is too high causing
> fluid to leak out of the vasculature....ideally you want to push the blood
> out through the the hole you made in the right atrium, not through the
> walls of the vessels. at what rate do you perfuse? if this happens a lot
> slow it down.
>
> Hope this helps.
>
> --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]"
> <thachdc <@t> niaid.nih.gov> wrote:
>
>> Hi Everyone!!
>>
>>    I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
>> nicking the upper right atrium of heart to collect the gushed out blood
>> and then perfusing through ventricle using a 21G butterfly needle and
>> peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes
>> out of mouth.  Occasionally, I see the liver fade to light pink.  Most of
>> the time the paws become white.  But the brain and spinal cord (my
>> tissues of interest) are always white, and seem to have been perfused.  I
>> was wondering how to improve this to get more consistent good perfusions,
>> and what signs should I look for to indicate good perfusion?
>>
>> Thanks much,
>>
>> Dzung
>> NIAID
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> Merced M Leiker
> Research Technician II
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY  14214
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT)
> From: Steven Coakley <sjchtascp <@t> yahoo.com>
> Subject: [Histonet] WI/MI
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <385293.93112.qm <@t> web38204.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I'm looking for a few techs from Northern WI or Northern MI to let me know 
> when HT positions become availiable.
>
> Thanks
>
>
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 67, Issue 32
> ****************************************
>