Hi everyone, Has anyone had problems with TTF-1 not staining Bouin's fixed tissue. We stain ours on the DAKO Autostainer with Envision+ Dual Link HRP System. We use DAKO pH9 target retrieval in the pressure cooker, and use a 1:50 dilution of DAKO TTF-1 (M3575). Ellen Yee Diagnostic Pathology Medical Group, Inc. Sacramento, CA