From d.a.faichney <@t> stir.ac.uk Mon Jun 1 04:12:00 2009 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Mon Jun 1 04:12:14 2009 Subject: [Histonet] Microtome Problems In-Reply-To: References: Message-ID: <8ED3F2CA5B78E142B8193376C57330F8E198E18F5C@EXCH2007.ad.stir.ac.uk> Sharon, There are little springs located behind the knife holder plate . If one of these is lost then it can cause the twisting that you describe. This happened to me years ago when stripping and cleaning one of our Leica 2035 microtome's. Hope this helps. Debbie Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Stirling Scotland UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Veterinary Services Laboratory Sent: 29 May 2009 15:12 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Problems Hello All I'm new to the group and histo. (3 years) so far I'm getting a lot of useful tips from all the messages. Thank you. I have two microtomes that were working almost perfectly before I had a technician service them. Now, the Leica 2035 Jung biocuts' blade holder is twisting to the side (going up at one end). I have tried tightening, slacking, and cleaning the entire assembly to no avail. Does anyone know of where I might be able to source one or even how to fix the one I have. Secondly, the Leitz 1512 rotary microtome, is now jumping forward and giving a loud clanking noise. It takes huge chunks out of the tissue. Any and all help is appreciated, Thank you Sharon Ms. Sharon Drayton Veterinary Services Laboratory Ministry of Agriculture & Rural Development The Pine St. Michael BB11091 Barbados Tel: (246) 427-5492 or (246) 427-5073 Fax: (246)426-7517 Email: vetlab@caribsurf.com Website: www.agriculture.gov.bb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Academic Excellence at the Heart of Scotland. The University of Stirling is a charity registered in Scotland, number SC 011159. From anh2006 <@t> med.cornell.edu Mon Jun 1 08:50:45 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Mon Jun 1 08:52:08 2009 Subject: [Histonet] RE: CD11c for murine tissu In-Reply-To: <21D20198BF6341BA96FB25ABD32ECF65@prueggihctechlt> References: <000501c9e135$3c559dd0$b500d970$%callis@bresnan.net><21D20198BF6341BA96FB25ABD32ECF65@prueggihctechlt> Message-ID: <726660407-1243864322-cardhu_decombobulator_blackberry.rim.net-1774812037-@bxe1087.bisx.prod.on.blackberry> Correct me if I am wrong but CD11c is predominantly on dendritic cells and F4/80 is for predominantly macrophage/myeloid populations (therefore a good CD11b substitute). Are you sure you should use F4/80 as a CD11c substitute? -----Original Message----- From: Patsy Ruegg Date: Sat, 30 May 2009 10:44:14 To: 'gayle callis'; 'Histonet' Subject: RE: [Histonet] RE: CD11c for murine tissu An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use from Serotec, it works in frozen and ffpe tissue. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Saturday, May 30, 2009 8:45 AM To: 'Histonet' Subject: [Histonet] RE: CD11c for murine tissu You wrote: Hi all - I'm looking for a good antibody to use on mouse tissue (frozen) to look for CD11c - does anyone have a suggestion for a good antibody source? Thanks in advance - Melissa We us CD11c, HL3 clone, Armenian Hamster anti Mouse. Negative control is Armenian Hamster IgG1 from BD Pharmingen/Invitrogen. Tissues are fresh frozen sections fixed with our favorite acetone/absolute ethanol fixative, although you can use 4C acetone for 10 min on overnight air dried sections. I will be happy to send the other fixative recipe if you want it. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Simoskevitz <@t> Osteotech.com Mon Jun 1 09:34:23 2009 From: Simoskevitz <@t> Osteotech.com (Simoskevitz@Osteotech.com) Date: Mon Jun 1 09:33:41 2009 Subject: [Histonet] Re: Modified Oserhoff Massons Trichrome In-Reply-To: <20090530170222.ADB2D20AC586@l3inpf3.contentcatcher.com> Message-ID: Gayle, I would love if you could find this protocol. It is definitely worth a try! Thanks, Ricki ********************************************* Ricki Simoskevitz, Research Associate III Osteotech Inc. 51 James Way Eatontown, NJ 07724 Phone: (732) 542-2800 X6328 Fax: (732) 935-1298 ********************************************** From Simoskevitz <@t> Osteotech.com Mon Jun 1 09:45:42 2009 From: Simoskevitz <@t> Osteotech.com (Simoskevitz@Osteotech.com) Date: Mon Jun 1 09:44:58 2009 Subject: [Histonet] Processing times In-Reply-To: <20090530170222.ADB2D20AC586@l3inpf3.contentcatcher.com> Message-ID: I am looking for a good processing schedule for demineralized rat femurs and liver samples. We have a Sakura Tissue-Tek VIP processor. Our lab would like to start doing paraffin sections and I am not sure what processing schedule works best for these samples. Thanks for any and all help. Ricki ********************************************* Ricki Simoskevitz, Research Associate III Osteotech Inc. 51 James Way Eatontown, NJ 07724 Phone: (732) 542-2800 X6328 Fax: (732) 935-1298 ********************************************** From pruegg <@t> ihctech.net Mon Jun 1 10:09:30 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jun 1 10:09:37 2009 Subject: [Histonet] RE: CD11c for murine tissu In-Reply-To: <726660407-1243864322-cardhu_decombobulator_blackberry.rim.net-1774812037-@bxe1087.bisx.prod.on.blackberry> References: <000501c9e135$3c559dd0$b500d970$%callis@bresnan.net><21D20198BF6341BA96FB25ABD32ECF65@prueggihctechlt> <726660407-1243864322-cardhu_decombobulator_blackberry.rim.net-1774812037-@bxe1087.bisx.prod.on.blackberry> Message-ID: <9E06F6F6DD5E48DF8859F7C0C51E387E@Patsyoffice> I beg your pardon, you are correct, I was thinking of cd11b not cd11c, as F4/80 is for macs not dendritic cells. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Monday, June 01, 2009 7:51 AM To: Patsy Ruegg; histonet-bounces@lists.utsouthwestern.edu; 'gayle callis'; 'Histonet' Subject: Re: [Histonet] RE: CD11c for murine tissu Correct me if I am wrong but CD11c is predominantly on dendritic cells and F4/80 is for predominantly macrophage/myeloid populations (therefore a good CD11b substitute). Are you sure you should use F4/80 as a CD11c substitute? -----Original Message----- From: Patsy Ruegg Date: Sat, 30 May 2009 10:44:14 To: 'gayle callis'; 'Histonet' Subject: RE: [Histonet] RE: CD11c for murine tissu An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use from Serotec, it works in frozen and ffpe tissue. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Saturday, May 30, 2009 8:45 AM To: 'Histonet' Subject: [Histonet] RE: CD11c for murine tissu You wrote: Hi all - I'm looking for a good antibody to use on mouse tissue (frozen) to look for CD11c - does anyone have a suggestion for a good antibody source? Thanks in advance - Melissa We us CD11c, HL3 clone, Armenian Hamster anti Mouse. Negative control is Armenian Hamster IgG1 from BD Pharmingen/Invitrogen. Tissues are fresh frozen sections fixed with our favorite acetone/absolute ethanol fixative, although you can use 4C acetone for 10 min on overnight air dried sections. I will be happy to send the other fixative recipe if you want it. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cruffin <@t> valleycare.com Mon Jun 1 11:23:06 2009 From: cruffin <@t> valleycare.com (Carol Ruffin) Date: Mon Jun 1 11:23:14 2009 Subject: [Histonet] IHC Stainers..... Message-ID: <4A239DFA020000F200001CE7@mail.valleycare.com> I am working for a small volume lab and we are looking for an IHC stainer. I have not been anywhere to see what is out there and would really like some neutral opinions, regarding experience or knowledge of products on the market right to get an idea of what direction to go in. We are using a Dako Artisan for both special stains and IHC now but will not be able to get antibodies for this stainer in the future. Ideally, we would like to be able to do both, but would be happy with some solid info on just an IHC stainer. Nothing at all against Dako....great instrument...but my boss would like to have more choices. Thank you, Carole Ruffin HT (ASCP) This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From rjbuesa <@t> yahoo.com Mon Jun 1 11:27:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 1 11:27:08 2009 Subject: [Histonet] IHC Stainers..... Message-ID: <44696.1451.qm@web65709.mail.ac4.yahoo.com> Try to get in contact with a Leica Microsystems representative to find out about the BondMax. Great instrument. Ren? J. --- On Mon, 6/1/09, Carol Ruffin wrote: From: Carol Ruffin Subject: [Histonet] IHC Stainers..... To: histonet@lists.utsouthwestern.edu Date: Monday, June 1, 2009, 12:23 PM I am working for a small volume lab and we are looking for an IHC stainer. I have not been anywhere to see what is out there and would really like some neutral opinions, regarding experience or knowledge of products on the market right to get an idea of what direction to go in. We are using a Dako Artisan for both special stains and IHC now but will not be able to get antibodies for this stainer in the future. Ideally, we would like to be able to do both, but would be happy with some solid info on just an IHC stainer. Nothing at all against Dako....great instrument...but my boss would like to have more choices.? ? ? ? ? ???Thank you, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Carole Ruffin HT (ASCP) This message and any included attachments are from ValleyCare Health System? and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Jun 1 11:52:54 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jun 1 11:52:05 2009 Subject: [Histonet] IHC Stainers..... In-Reply-To: <4A239DFA020000F200001CE7@mail.valleycare.com> References: <4A239DFA020000F200001CE7@mail.valleycare.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088A7D3131@IBMB7Exchange.digestivespecialists.com> Carole, I am using BioCare's Intellipath and like it very much. It is an open stainer so you can use what ever reagents you want to use but BioCare's cost for reagents is excellent. Also, it has a continuous access so adding more slides while a run is in process is not a problem. That's great when you have a pathologist that as soon as you have a run on decides he wants another stain. It also has a stat run capability. The technical and sales support staff is excellent. If you would like any more information you can contact me off line. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Ruffin Sent: Monday, June 01, 2009 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Stainers..... I am working for a small volume lab and we are looking for an IHC stainer. I have not been anywhere to see what is out there and would really like some neutral opinions, regarding experience or knowledge of products on the market right to get an idea of what direction to go in. We are using a Dako Artisan for both special stains and IHC now but will not be able to get antibodies for this stainer in the future. Ideally, we would like to be able to do both, but would be happy with some solid info on just an IHC stainer. Nothing at all against Dako....great instrument...but my boss would like to have more choices. Thank you, Carole Ruffin HT (ASCP) This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 1 12:44:17 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 1 12:44:22 2009 Subject: [Histonet] RPMI Message-ID: <65365F35C0F2EF4D846EC3CA73E49C437A9BFA9804@HPEMX3.HealthPartners.int> What is the correct way to store RPMI after placing a tissue sample in it, refrigerated or ambient? Thanks for your educated assistance!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From joseph-galbraith <@t> uiowa.edu Mon Jun 1 13:07:25 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Jun 1 13:07:30 2009 Subject: [Histonet] RPMI In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C437A9BFA9804@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C437A9BFA9804@HPEMX3.HealthPartners.int> Message-ID: Dorothy: There is a split in opinion with the pathologists on our staff but the younger and more broadly based staff (meaning multiple institution background) are adamant that the appropriate way to temporarily store tissue (not blood) is at refrigerator temp (preferably wet ice initially). I would also be interested to hear what others are saying. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, June 01, 2009 12:44 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RPMI What is the correct way to store RPMI after placing a tissue sample in it, refrigerated or ambient? Thanks for your educated assistance!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From n.singh <@t> biogenex.com Mon Jun 1 14:59:53 2009 From: n.singh <@t> biogenex.com (Neeraj K. Singh) Date: Mon Jun 1 15:03:55 2009 Subject: [Histonet] IHC Stainers In-Reply-To: <20090601170209.72B0611E425@barracuda.biogenex.com> References: <20090601170209.72B0611E425@barracuda.biogenex.com> Message-ID: <37DC9F93CF7F864182D0463EF93D571B9D433B@ISLETON2.california.biogenex.com> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 01, 2009 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 67, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: muscle striations (Tony Henwood) 2. RE: Peroxidase block again................ (Tony Henwood) 3. problem with attaching MMA section to glass slide (ooi.ting.huay@nhc.com.sg) 4. RE: MAP-2 antibody (Jim Manavis) 5. RE: Microtome Problems (Deborah Faichney) 6. Re: RE: CD11c for murine tissu (anh2006@med.cornell.edu) 7. Re: Modified Oserhoff Massons Trichrome (Simoskevitz@Osteotech.com) 8. Processing times (Simoskevitz@Osteotech.com) 9. RE: RE: CD11c for murine tissu (Patsy Ruegg) 10. IHC Stainers..... (Carol Ruffin) 11. Re: IHC Stainers..... (Rene J Buesa) 12. RE: IHC Stainers..... (Blazek, Linda) ---------------------------------------------------------------------- Message: 1 Date: Mon, 1 Jun 2009 09:10:48 +1000 From: "Tony Henwood" Subject: RE: [Histonet] muscle striations To: "Edwards, R.E." , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-7" Richard, Cherukian's Phosphotungstic Acid Haematoxylin works well Principle: Cherukian's modification employs an eosin solution that stains the erythrocytes red and differentiates them from the blue fibrin. Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5?m. Controls: Use brain sections and section of muscle. A good stain will demonstrate the dendrites as blue where as in a bad stain they appear light grey to salmon in colour. Nuclei, fibrin, platelets and muscle will be blue, red cells and collagen appear red. Muscle striations should be well defined. Reagents: 1. Stock Eosin: - Warning: Flammable - see MSDS Eosin Y, water soluble (CI 45380) 0.5g Distilled Water 10ml 80% Ethanol 190ml 2. Working Eosin Solution: Stock Eosin 10ml Before use add 50ul glacial acetic acid. 3. 1% Periodic Acid 4. PTAH solution Haematoxylin (CI 75290) 0.5g Phosphotungstic Acid 10g Distilled water 500ml Dissolve solid ingredients in separate portions of the water. Use gentle heat for Haematoxylin. Combine solutions when cool. Add 0.088g potassium permanganate to ripen. The stain is ready to use. Procedure: 1. Dewax and hydrate sections to 80% alcohol. 2. Place slides in working eosin solution for 30 seconds. 3. Wash slides in distilled water for a few seconds. 4. Place slides in 1% periodic acid for 20 minutes. 5. Wash slides in water for 3 minutes. 6. Place slides in PTAH solution for 30-90 minutes in 60oC oven. Check from 30 minutes on. 7. Dehydrate, clear and mount. Results: Dendrites, nuclei, fibrin, platelets and muscle - blue Red blood cells and collagen - red. Notes: Reference: 1. , C.J., Histologic. 8(4); 105, (1977). 2. Luna, L., Histologic. 5(2); 66, (1975). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Thursday, 28 May 2009 6:29 PM To: Histonet Subject: [Histonet] muscle striations Any favourite methods for the above?? I'm aware of Heidenhain's and Mallory's PTAH. Many thanks Richard Edwards University of Leicester _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 2 Date: Mon, 1 Jun 2009 09:51:33 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Peroxidase block again................ To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Yep, You can use alkaline phosphatase labelling instead of peroxidase Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com Sent: Friday, 29 May 2009 9:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peroxidase block again................ good morning, ???? I would like to pose this questions again...Is there anyone out there who has eliminated using Peroxidase Block on either IHC or ICC? Your feedback is very helpful. Thanks, Gene Cleveland Clinic _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 3 Date: Mon, 1 Jun 2009 09:23:04 +0800 From: ooi.ting.huay@nhc.com.sg Subject: [Histonet] problem with attaching MMA section to glass slide To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" To all histonetters, I am currently working with the MMA embedded section. I need to attach about 100micro thick sample to glass slide prior to staining. The sample is direct from saw microtome. Secure attached is needed so that the section will not detach from glass slide while staining. I have tried the Haupts adhesive coated slides and bond-rite slides. It doesnt work very well... Please advice and share your expertists. Any suggestion and feedback are welcome. Thank you very much! Regards, Ooi _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer ------------------------------ Message: 4 Date: Mon, 1 Jun 2009 12:25:50 +0930 From: Jim Manavis Subject: RE: [Histonet] MAP-2 antibody To: "'Lott, Robert'" , Histonet Message-ID: <003e01c9e264$78bfea20$2c6c140a@41984n> Content-Type: text/plain; charset="us-ascii" Robert If you are using formalin fixed paraffin embedded human material, Sigma have a very nice mouse monoclonal that works beautifully. The cat no. is M-4403 (clone HM-2). Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Saturday, 30 May 2009 2:05 AM To: histonet@lists.utsouthwestern.edu Cc: Christa Hladik Subject: [Histonet] MAP-2 antibody Hi Everyone, One of our pathologists is interested in MAP-2 IHC for the following application: Microtubule Associated Protein-2 Is a Sensitive Marker of Primary and Metastatic Neuroblastoma. Krishnan C, Heerema-McKenney A, Arber DA. Department of Pathology, Stanford University, Stanford, CA. Comment: Microtubule associated protein-2 (MAP-2) is a protein expressed in high levels in cells derived from the neural crest. It is a very robust marker of neuronal differentiation and has been used most often in evaluating neuronal differentiation in tumors of the central nervous system. MAP-2 showed comparable sensitivity in staining primary neuroblastomas (pre- and post-treatment samples) as compared to synaptophysin, chromogranin, CD56, and beta-catenin. In contrast to other markers of neuroblastoma, MAP-2 did not show significant cross reactivity to native bone marrow precursors, thus eliminating a potential source of confusion. Does anyone use this antibody for this or like applications.... I have looked and am "fairly" sure that there is not an IVD available, but would still be interested in knowing which antibody you use. Thanks, Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213/ 205-592-5387 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 1 Jun 2009 10:12:00 +0100 From: Deborah Faichney Subject: RE: [Histonet] Microtome Problems To: 'Veterinary Services Laboratory' , "histonet@lists.utsouthwestern.edu" Message-ID: <8ED3F2CA5B78E142B8193376C57330F8E198E18F5C@EXCH2007.ad.stir.ac.uk> Content-Type: text/plain; charset="us-ascii" Sharon, There are little springs located behind the knife holder plate . If one of these is lost then it can cause the twisting that you describe. This happened to me years ago when stripping and cleaning one of our Leica 2035 microtome's. Hope this helps. Debbie Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Stirling Scotland UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Veterinary Services Laboratory Sent: 29 May 2009 15:12 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Problems Hello All I'm new to the group and histo. (3 years) so far I'm getting a lot of useful tips from all the messages. Thank you. I have two microtomes that were working almost perfectly before I had a technician service them. Now, the Leica 2035 Jung biocuts' blade holder is twisting to the side (going up at one end). I have tried tightening, slacking, and cleaning the entire assembly to no avail. Does anyone know of where I might be able to source one or even how to fix the one I have. Secondly, the Leitz 1512 rotary microtome, is now jumping forward and giving a loud clanking noise. It takes huge chunks out of the tissue. Any and all help is appreciated, Thank you Sharon Ms. Sharon Drayton Veterinary Services Laboratory Ministry of Agriculture & Rural Development The Pine St. Michael BB11091 Barbados Tel: (246) 427-5492 or (246) 427-5073 Fax: (246)426-7517 Email: vetlab@caribsurf.com Website: www.agriculture.gov.bb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Academic Excellence at the Heart of Scotland. The University of Stirling is a charity registered in Scotland, number SC 011159. ------------------------------ Message: 6 Date: Mon, 1 Jun 2009 13:50:45 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] RE: CD11c for murine tissu To: "Patsy Ruegg" , histonet-bounces@lists.utsouthwestern.edu, "'gayle callis'" , "'Histonet'" Message-ID: <726660407-1243864322-cardhu_decombobulator_blackberry.rim.net-1774812037-@bxe1087.bisx.prod.on.blackberry> Content-Type: text/plain Correct me if I am wrong but CD11c is predominantly on dendritic cells and F4/80 is for predominantly macrophage/myeloid populations (therefore a good CD11b substitute). Are you sure you should use F4/80 as a CD11c substitute? -----Original Message----- From: Patsy Ruegg Date: Sat, 30 May 2009 10:44:14 To: 'gayle callis'; 'Histonet' Subject: RE: [Histonet] RE: CD11c for murine tissu An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use from Serotec, it works in frozen and ffpe tissue. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Saturday, May 30, 2009 8:45 AM To: 'Histonet' Subject: [Histonet] RE: CD11c for murine tissu You wrote: Hi all - I'm looking for a good antibody to use on mouse tissue (frozen) to look for CD11c - does anyone have a suggestion for a good antibody source? Thanks in advance - Melissa We us CD11c, HL3 clone, Armenian Hamster anti Mouse. Negative control is Armenian Hamster IgG1 from BD Pharmingen/Invitrogen. Tissues are fresh frozen sections fixed with our favorite acetone/absolute ethanol fixative, although you can use 4C acetone for 10 min on overnight air dried sections. I will be happy to send the other fixative recipe if you want it. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 1 Jun 2009 10:34:23 -0400 From: Simoskevitz@Osteotech.com Subject: [Histonet] Re: Modified Oserhoff Massons Trichrome To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Gayle, I would love if you could find this protocol. It is definitely worth a try! Thanks, Ricki ********************************************* Ricki Simoskevitz, Research Associate III Osteotech Inc. 51 James Way Eatontown, NJ 07724 Phone: (732) 542-2800 X6328 Fax: (732) 935-1298 ********************************************** ------------------------------ Message: 8 Date: Mon, 1 Jun 2009 10:45:42 -0400 From: Simoskevitz@Osteotech.com Subject: [Histonet] Processing times To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I am looking for a good processing schedule for demineralized rat femurs and liver samples. We have a Sakura Tissue-Tek VIP processor. Our lab would like to start doing paraffin sections and I am not sure what processing schedule works best for these samples. Thanks for any and all help. Ricki ********************************************* Ricki Simoskevitz, Research Associate III Osteotech Inc. 51 James Way Eatontown, NJ 07724 Phone: (732) 542-2800 X6328 Fax: (732) 935-1298 ********************************************** ------------------------------ Message: 9 Date: Mon, 1 Jun 2009 09:09:30 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] RE: CD11c for murine tissu To: , , "'gayle callis'" , "'Histonet'" Message-ID: <9E06F6F6DD5E48DF8859F7C0C51E387E@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I beg your pardon, you are correct, I was thinking of cd11b not cd11c, as F4/80 is for macs not dendritic cells. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Monday, June 01, 2009 7:51 AM To: Patsy Ruegg; histonet-bounces@lists.utsouthwestern.edu; 'gayle callis'; 'Histonet' Subject: Re: [Histonet] RE: CD11c for murine tissu Correct me if I am wrong but CD11c is predominantly on dendritic cells and F4/80 is for predominantly macrophage/myeloid populations (therefore a good CD11b substitute). Are you sure you should use F4/80 as a CD11c substitute? -----Original Message----- From: Patsy Ruegg Date: Sat, 30 May 2009 10:44:14 To: 'gayle callis'; 'Histonet' Subject: RE: [Histonet] RE: CD11c for murine tissu An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use from Serotec, it works in frozen and ffpe tissue. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Saturday, May 30, 2009 8:45 AM To: 'Histonet' Subject: [Histonet] RE: CD11c for murine tissu You wrote: Hi all - I'm looking for a good antibody to use on mouse tissue (frozen) to look for CD11c - does anyone have a suggestion for a good antibody source? Thanks in advance - Melissa We us CD11c, HL3 clone, Armenian Hamster anti Mouse. Negative control is Armenian Hamster IgG1 from BD Pharmingen/Invitrogen. Tissues are fresh frozen sections fixed with our favorite acetone/absolute ethanol fixative, although you can use 4C acetone for 10 min on overnight air dried sections. I will be happy to send the other fixative recipe if you want it. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi Carol, BioGenex i6000 is an ideal autostainer for lab having both IHC and SS work load. It does both applications all in one all at once. Have continous and STAT features for loading fresh slides with those already onboard for staining. 60 slides and 60 reagents capacity. Software completely open with research key to create user own protocol like IHC, SS,IF etc. Open for primary, detection kit etc. Thanks Neeraj ------------------------------ Message: 10 Date: Mon, 01 Jun 2009 09:23:06 -0700 From: "Carol Ruffin" Subject: [Histonet] IHC Stainers..... To: Message-ID: <4A239DFA020000F200001CE7@mail.valleycare.com> Content-Type: text/plain; charset=US-ASCII I am working for a small volume lab and we are looking for an IHC stainer. I have not been anywhere to see what is out there and would really like some neutral opinions, regarding experience or knowledge of products on the market right to get an idea of what direction to go in. We are using a Dako Artisan for both special stains and IHC now but will not be able to get antibodies for this stainer in the future. Ideally, we would like to be able to do both, but would be happy with some solid info on just an IHC stainer. Nothing at all against Dako....great instrument...but my boss would like to have more choices. Thank you, Carole Ruffin HT (ASCP) This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. ------------------------------ Message: 11 Date: Mon, 1 Jun 2009 09:27:01 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Stainers..... To: histonet@lists.utsouthwestern.edu, Carol Ruffin Message-ID: <44696.1451.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try to get in contact with a Leica Microsystems representative to find out about the BondMax. Great instrument. Ren? J. --- On Mon, 6/1/09, Carol Ruffin wrote: From: Carol Ruffin Subject: [Histonet] IHC Stainers..... To: histonet@lists.utsouthwestern.edu Date: Monday, June 1, 2009, 12:23 PM I am working for a small volume lab and we are looking for an IHC stainer. I have not been anywhere to see what is out there and would really like some neutral opinions, regarding experience or knowledge of products on the market right to get an idea of what direction to go in. We are using a Dako Artisan for both special stains and IHC now but will not be able to get antibodies for this stainer in the future. Ideally, we would like to be able to do both, but would be happy with some solid info on just an IHC stainer. Nothing at all against Dako....great instrument...but my boss would like to have more choices.? ? ? ? ? ???Thank you, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Carole Ruffin HT (ASCP) This message and any included attachments are from ValleyCare Health System? and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 1 Jun 2009 12:52:54 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] IHC Stainers..... To: 'Carol Ruffin' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39088A7D3131@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Carole, I am using BioCare's Intellipath and like it very much. It is an open stainer so you can use what ever reagents you want to use but BioCare's cost for reagents is excellent. Also, it has a continuous access so adding more slides while a run is in process is not a problem. That's great when you have a pathologist that as soon as you have a run on decides he wants another stain. It also has a stat run capability. The technical and sales support staff is excellent. If you would like any more information you can contact me off line. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Ruffin Sent: Monday, June 01, 2009 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Stainers..... I am working for a small volume lab and we are looking for an IHC stainer. I have not been anywhere to see what is out there and would really like some neutral opinions, regarding experience or knowledge of products on the market right to get an idea of what direction to go in. We are using a Dako Artisan for both special stains and IHC now but will not be able to get antibodies for this stainer in the future. Ideally, we would like to be able to do both, but would be happy with some solid info on just an IHC stainer. Nothing at all against Dako....great instrument...but my boss would like to have more choices. Thank you, Carole Ruffin HT (ASCP) This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 1 *************************************** From edpace96 <@t> yahoo.com Mon Jun 1 16:31:38 2009 From: edpace96 <@t> yahoo.com (Eric Pace) Date: Mon Jun 1 16:31:41 2009 Subject: [Histonet] (no subject) Message-ID: <609443.18206.qm@web80006.mail.sp1.yahoo.com> Hello, ? I am a histotech in Houston and I am currently look for a opening in the Austin,Tx area.? Have been a supervisor since January of this year but I have about 4 yrs of histology experience.? I have my HT and I would like to get my QIHC sometime in the future.? I am not looking for a supervisor job I would much rather just be a regular histo tech.? If anyone knows or hears of an opening please let me know. ? Thanks, Eric Pace BS, HT (ASCP)cm From AnthonyH <@t> chw.edu.au Mon Jun 1 18:33:34 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jun 1 18:33:50 2009 Subject: [Histonet] Peroxidase block again................ In-Reply-To: <8CBB0AE4CA4CB0E-C04-57C6@webmail-mh36.sysops.aol.com> Message-ID: Gene, A few references that might be of use: Jylling et al "Immunohistochemistry on Frozen Section of Sentinel Lymph Nodes in Breast Cancer With Improved Morphology and Blocking of Endogenous Peroxidase" Appl Immunohistochem Mol Morphol 2008;16:482-484 - Simultaneous blocking of endogenous peroxidase and antigen retrieval was carried out by microwaving at 1000W in TRIS-EGTA buffer, pH 9,0 preheated to 601C until boiling point was reached (approximately 30 s). And: Gao et al "Microwave Antigen Retrieval Blocks Endogenous Peroxidase Activity in Immunohistochemistry" Appl Immunohistochem Mol Morphol 2008;16:393-399 - suggesting that microwave exposure (HIER) is able to block the endogenous peroxidase. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: njoydobro@aol.com [mailto:njoydobro@aol.com] Sent: Monday, 1 June 2009 6:59 PM To: Tony Henwood Subject: Re: [Histonet] Peroxidase block again................ Can't use it. -----Original Message----- From: Tony Henwood To: njoydobro@aol.com; Histonet@lists.utsouthwestern.edu Sent: Sun, 31 May 2009 7:51 pm Subject: RE: [Histonet] Peroxidase block again................ Yep, You can use alkaline phosphatase labelling instead of peroxidase Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com Sent: Friday, 29 May 2009 9:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peroxidase block again................ good morning, ???? I would like to pose this questions again...Is there anyone out there who has eliminated using Peroxidase Block on either IHC or ICC? Your feedback is very helpful. Thanks, Gene Cleveland Clinic _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** A Good Credit Score is 700 or Above. See yours in just 2 easy steps! ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From sbreeden <@t> nmda.nmsu.edu Tue Jun 2 10:45:15 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jun 2 10:45:20 2009 Subject: [Histonet] Rene Buesa - Please contact Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46887@nmdamailsvr.nmda.ad.nmsu.edu> Rene: I have a survey for you and need your email address, please. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Maria.Katleba <@t> stjoe.org Tue Jun 2 12:46:43 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Jun 2 12:46:52 2009 Subject: [Histonet] Histotech needed - Napa CA 94558 Message-ID: <97C02552ECB11346877D3E83CF833ABD13CC23A8D3@SJSNT-SCMAIL03.stjoe.org> Looking for Histotech FT Licensed 5+ years experience Routine Histology...IHC...Special Stains...etc 2am shift, 40 hours, benefits Fun atmosphere!!!!! Please send resume by mail or FAX 707-257-4076 www.thequeen.org to apply online Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 707-294-9229 cell 707-252-4411 x3689 Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From TJJ <@t> stowers.org Tue Jun 2 13:09:40 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Jun 2 13:10:30 2009 Subject: [Histonet] Re: Peroxidase block again.... Message-ID: Gene, Why would you want to eliminate peroxidase blocking as part of your immunostaining procedure? It's cheap, it's quick, it's easy, and if you think it interferes with the ability of the primary antibody to bind with the antigen, you add it as a step after application of the primary antibody. Simply use aqueous hydrogen peroxide instead of methanolic routinely, and you won't have to worry about the potential of methanol to interfere with the immunoreactivity of the tissues for CD markers. You absolutely can leave it out and learn to ignore the positive staining of the RBCs and other tissues, and control what's labeled endogenous staining by including a negative control. Frankly, unless there is a scientific reason to have it included (i.e. looking for antigen positivity (demonstrated by a different enzyme) in cells/tissues which contain endogenous peroxidase), it's best to get rid of it. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Nikki.Wahlberg <@t> bsci.com Tue Jun 2 13:19:49 2009 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Tue Jun 2 13:19:52 2009 Subject: [Histonet] MMA Dehydration Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E243E@MAPMAIL02.bsci.bossci.com> Hello Everyone, I am wondering if anyone would be willing to share their MMA dehydration schedules? We are currently trying to improve our EXAKT MMA specimens. We currently process with the following schedule: 10% Formalin 4 hours 70% Ethyl Alcohol 1 hour 95% Ethyl Alcohol 2 hours 95% Ethyl Alcohol 2 hours 100% Ethyl Alcohol 3 hours 100% Ethyl Alcohol 3 hours Thank you, Nikki Nikki M Wahlberg Histotechnologist II Histo-Pathology Services Boston Scientific 763-694-5739 wahlbern@bsci.com CONFIDENTIALITY NOTICE: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. Any unauthorized distribution or copying of this transmittal or its attachments, if any, is prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Boston Scientific Corporation can arrange for proper delivery, then please delete the message from your inbox. From Nikki.Wahlberg <@t> bsci.com Tue Jun 2 13:50:20 2009 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Tue Jun 2 13:50:24 2009 Subject: [Histonet] MMA Dehydration In-Reply-To: References: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E243E@MAPMAIL02.bsci.bossci.com> Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E243F@MAPMAIL02.bsci.bossci.com> We are mainly processing vessels that can range in size of 10mm-200mm in length. The different types of vessels are either peripheral, cardiac or pulmonary trunks. Nikki ________________________________ From: Jack Ratliff [mailto:ratliffjack@hotmail.com] Sent: Tuesday, June 02, 2009 1:27 PM To: Wahlberg, Nikki Subject: RE: [Histonet] MMA Dehydration What is the tissue you are wanting to process??? Jack Ratliff > Date: Tue, 2 Jun 2009 13:19:49 -0500 > From: Nikki.Wahlberg@bsci.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] MMA Dehydration > > Hello Everyone, > > I am wondering if anyone would be willing to share their MMA dehydration > schedules? We are currently trying to improve our EXAKT MMA specimens. > We currently process with the following schedule: > 10% Formalin 4 hours > 70% Ethyl Alcohol 1 hour > 95% Ethyl Alcohol 2 hours > 95% Ethyl Alcohol 2 hours > 100% Ethyl Alcohol 3 hours > 100% Ethyl Alcohol 3 hours > > Thank you, > Nikki > > Nikki M Wahlberg > Histotechnologist II > Histo-Pathology Services > Boston Scientific > 763-694-5739 > wahlbern@bsci.com > > CONFIDENTIALITY NOTICE: This e-mail transmission may contain > confidential or legally privileged information that is intended only for > the individual or entity named in the e-mail address. Any unauthorized > distribution or copying of this transmittal or its attachments, if any, > is prohibited. If you have received this e-mail transmission in error, > please reply to the sender, so that Boston Scientific Corporation can > arrange for proper delivery, then please delete the message from your > inbox. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbruce <@t> vetpathservicesinc.com Tue Jun 2 16:20:09 2009 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Tue Jun 2 16:20:17 2009 Subject: [Histonet] Autoradiography Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A285528B@vpss1.VetPathServicesInc.local> Does any one know of a company that does Autoradiography (Tritium) on rabbit eyes? Thanks so much in advance, Suzanne _______________________________ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator From dholmes <@t> anatomy.umsmed.edu Tue Jun 2 16:43:46 2009 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Tue Jun 2 16:44:13 2009 Subject: [Histonet] microtome calibration Message-ID: <4A2556C2020000820003AA85@GWIA1.umsmed.edu> Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From NSEARCY <@t> swmail.sw.org Tue Jun 2 17:07:21 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Jun 2 17:07:29 2009 Subject: [Histonet] Technical EM Message-ID: <4A255C49.5D38.00EF.0@swmail.sw.org> Am in desperate need of technical ONLY electron microscopy studies. Cut embedded block, take pics and return to me for pathologist interpretation. Anyone know of such a service? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From cfarish <@t> csu.edu.au Tue Jun 2 19:18:02 2009 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Tue Jun 2 19:18:24 2009 Subject: [Histonet] TSH receptor Message-ID: <79A000B60AFE8045BA2581119DFEC444058A3F66@ESWW01.CSUMain.csu.edu.au> Hi Folks, I'm looking for a TSH receptor antibody suitable for IHC in FFPE tissue from sheep. I'm struggling to find anything that may be applicable outside of the human field. Any suggestions would be most welcome. As always, thanks to all, Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW Australia "I don't want to achieve immortality through my work. I want to achieve immortality through not dying" Woody Allen From akemiat3377 <@t> yahoo.com Tue Jun 2 21:16:45 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Jun 2 21:16:50 2009 Subject: [Histonet] Fw: Director, Laboratory Operations Message-ID: <274521.78156.qm@web31308.mail.mud.yahoo.com> Hi All, I was sent this opportunity for a Lab Operations position, but I already have a new job. ?I thought I would pass it on to anyone interested. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com --- On Tue, 6/2/09, Steve Merwin wrote: From: Steve Merwin Subject: Director, Laboratory Operations To: akemiat3377@yahoo.com Date: Tuesday, June 2, 2009, 1:45 PM Dear Akemi, We have been retained by a dynamic and cutting edge molecular diagnostics based division of a Life Sciences company that provides competitive and personalized diagnosis toward cancer treatment. The company, which is well funded and financially stable, has retained us to help identify leaders for a Director of Laboratory Operations position that will be based in Southern California.? This important and visible position within the organization will have responsibility for overseeing multiple clinical lab departments including flow cytometry, molecular diagnostics and client services comprising a group of up to 50. We are looking for candidates that possess the following: ????????? BA/BS in Chemistry, Biology, Microbiology, Medical Technology or related field required. Advanced scientific degree desirable. ????????? 10+ years post grad/industry experience. ????????? 7+ years supervisory experience with a group of 30+, preferable in a commercial clinical laboratory. Experience overseeing molecular diagnostic testing would be viewed as a plus. ????????? Strong inter-departmental presence and strategic outlook. ????????? Experience ensuring regulatory compliance with federal and state agencies. The company offers an attractive compensation package as well as other benefits. Should you know of anyone who may be interested in hearing more about this opportunity, please contact me by telephone (925-472-6576) or reply by email. Thank you, in advance, for your time and help. Best regards, Steve Steve Merwin, C.P.C. Senior Executive Search Consultant Sanford Rose Associates - San Francisco 1255 Treat Blvd., Suite 300 Walnut Creek, CA 94597 Phone: 925-472-6576 Fax: 925-472-6579 email: swm@srasf.com linkedin: http://www.linkedin.com/in/stevemerwin website: www.srasf.com "Finding People Who Make a Difference?? ? To be removed from our mailing list you may send a letter to the address listed above or send an email to remove@srasf.com? with "REMOVE" and include your "Original Email Address/Addresses" in the subject heading. ? ? ? ? From j.aflleje <@t> integrated-pathology.com Tue Jun 2 21:54:00 2009 From: j.aflleje <@t> integrated-pathology.com (James Aflleje) Date: Tue Jun 2 21:54:06 2009 Subject: [Histonet] Arizona histologist jobs Message-ID: <1BEBBEAB73EB8F40B2CEF99ACD5B33A7AA6757B0ED@Exchange.IPath.local> Hello fellow netters, I am inquiring about any open histologist positions for a friend of mine. She is well groomed in all fundamentals of the histology laboratory. And I highly recommend her. Any inquiries please contact me via this email. Thanks. James Aflleje HTL ASCP, Director of Laboratory, INTEGRATED-PATHOLOGY From wdesalvo.cac <@t> hotmail.com Tue Jun 2 22:45:28 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Jun 2 22:45:33 2009 Subject: [Histonet] NSH QC Committee - Call for Information Message-ID: I am looking for individuals to share their experience with Process Improvement in the Histology Lab. This year at the NSH Symposium/Convention, I would like to post information/examples/posters at the Committee table to help attendees better understand the successes possible and the difficulties faced when LEAN/SIX SIGMA/Total Quality Management tools and principles are applied to a Histology Lab. Additionally, I would like to create a resource group centered on the subject of Process Improvement in Histology and hopefully better understand the level of involvement and how many labs are using the tools to develop their workload/workflow and/or reduce defects/errors. Whatever your level of involvement or experience, I would appreciate hearing from you. Individuals working for Vendor companies are welcome to respond and participate. If you are interested in sharing your experience, please contact me using the e-mail listed below or at the NSH.org site/office. Thanks for your consideration of this request and I hope to see many of you at the convention in October. William DeSalvo, BS, HTL(ASCP) Committee Chair, NSH QC Committee wdesalvo.cac@hotmail.com _________________________________________________________________ Windows Live?: Keep your life in sync. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_BR_life_in_synch_062009 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Jun 3 09:31:27 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jun 3 09:31:32 2009 Subject: [Histonet] microtome calibration Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06E133C1@wahtntex2.waht.swest.nhs.uk> Have you tried altering the angle of the knife? Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes Sent: 02 June 2009 22:44 To: Histonet Subject: [Histonet] microtome calibration Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Wed Jun 3 12:45:44 2009 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jun 3 12:46:13 2009 Subject: [Histonet] Intellipath LIS Message-ID: <379A927A452F3D43A3C8705F4E67905F0B44995478@EX05.net.ucsf.edu> Hi everyone, Are any of you using the Intellipath LIS? Not the Intellipath stainer from Biocare. If so, I have some questions on how you use the procedures report to get your orders. Thanks! Erin Martin UCSF Dermatopathology From ndevans <@t> stanford.edu Wed Jun 3 12:58:56 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Jun 3 12:59:01 2009 Subject: [Histonet] Storage of tissues in PFA before dehydration and embedding Message-ID: <14194B8417FC4AF79169F8D859D42826@stanford.edu> Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University From CareerStudio <@t> aol.com Wed Jun 3 13:25:47 2009 From: CareerStudio <@t> aol.com (CareerStudio@aol.com) Date: Wed Jun 3 13:29:07 2009 Subject: [Histonet] Histology Manager for Clinical Lab in Connecticut Message-ID: Our client is a Connecticut-based clinical laboratory currently seeking a Laboratory Manager to oversee the operations of several departments within the Histology Lab. This individual will manage 50+ staff in a Lean environment, and be responsible for equipment, quality assurance, expense control, and managing the operating budget. This professional must have a BS or BA degree with 4+ years related Histology experience including demonstrated success efficiencly running a high volume, Lean laboratory, strong management background and meet CLIA requirements. Competitive starting salary and relocation assistance is offered. Please contact careerstudio@aol.com for more information. Barbara Siegel Career Studio national search Palm Beach, FL careerstudio@aol.com 561-738-6363 http://www.linkedin.com/in/careerstudio ************** Shop Inspiron, Studio and XPS Laptops at Dell.com (http://pr.atwola.com/promoclk/100126575x1222616459x1201464730/aol?redir=http:%2F%2Fad.d oubleclick.net%2Fclk%3B215218145%3B37264238%3Bd) From talulahgosh <@t> gmail.com Wed Jun 3 13:41:49 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jun 3 13:41:54 2009 Subject: [Histonet] Storage of tissues in PFA before dehydration and embedding In-Reply-To: <14194B8417FC4AF79169F8D859D42826@stanford.edu> References: <14194B8417FC4AF79169F8D859D42826@stanford.edu> Message-ID: We fix our embryos overnight in 4% para for in situ's--the long crosslinking helps fix the RNA better. For some immuno's though, too much fixing is definitely bad. Why not store in PBS until you're ready to dehydrate? Oh, (a note of caution?), we don't use para fixation for paraffin embedding, though--we use a much harsher fix, like modified Carnoy's or Serra's fix. When we fix with those, we store the embryos in 70% over the weekend (which is the next step after fixing for two to four hours--washing in 70% EtOH). These embryos can only be used with certain antibodies and very strong RNA probes. Btw, our tissue is young chick embryos--E2 to E8. Emily "Canned food is dead food....here kids can't study. They're going blind. Mothers are out in pants showing themselves off when they ought to be at home cooking fresh vegetables." --Charles Atlas, Saga magazine interview, 1964 From CIngles <@t> uwhealth.org Wed Jun 3 13:38:44 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jun 3 13:42:05 2009 Subject: [Histonet] H&E References: Message-ID: We are a large Mohs clinic (at least 50 mohs flats today, not counting verticals) We use Carazzi's Hematoxylin and the Eosin kit from Newcomer Supply. We use a linear stainer and it takes about 15-20 minutes. We don'rt fix in NBF, just alcohol. If you're interested, let me know. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Fri 5/29/2009 8:18 AM To: Histonet Subject: [Histonet] H&E POLL: What is everyone's favorite hematoxylin for work-horse H&E stains? What about eosin? I am re-evaluating the quality of our current set-up and think we can improve - especially for the frozen sections. Any suggestions/protocols/brand suggestions? Thanks, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Paul <@t> Firnschild.com Wed Jun 3 13:50:48 2009 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Wed Jun 3 13:50:53 2009 Subject: [Histonet] microtome calibration In-Reply-To: <4A2556C2020000820003AA85@GWIA1.umsmed.edu> Message-ID: <001001c9e47c$36ce7ef0$6402a8c0@qa44c0f386def9> Diane: I provide microtome service calibration etc. Please email me if I can be of service. References upon request. Paul@Firnschild.com Paul Paul M. Firnschild QA Support Services, Inc. 404.291.3715 -----Original Message----- From: Dianne Holmes [mailto:dholmes@anatomy.umsmed.edu] Sent: Tuesday, June 02, 2009 5:44 PM To: Histonet Subject: [Histonet] microtome calibration Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From alyssa <@t> alliedsearchpartners.com Wed Jun 3 14:30:09 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Jun 3 14:30:14 2009 Subject: [Histonet] Histology Positions-GA & PA Message-ID: Hello, We are seeking a Histotechnologist candidates for our client in *Evansburg**, **PA** (30 minutes northwest of **Philadelphia**) and in Augusta, GA.* This is an opportunity to work for a leading/well known company who plans on a 250% growth rate in the coming year. Our client has a state of the art lab, highly competitive compensation, and an excellent benefit plan. You can email me at alyssa@alliedsearchpartners.com with your interest. *TITLE/POSITION* Status: Exempt. Regular, full-time. Histotechnologist Work Shift: Monday-Friday, Day Shift, about 7:30am-3:30pm * * *QUALIFICATIONS* ASCP certification required Prior experience working as a histotech a must -- Alyssa Peterson, Director of Recruitment Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From leiker <@t> buffalo.edu Wed Jun 3 15:48:40 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Jun 3 15:49:04 2009 Subject: [Histonet] recommendations for anti-rat c-kit? Message-ID: <2AEEC8A35CFEC5D4E9F4FCA3@CDYwxp1931.ad.med.buffalo.edu> Can anyone recommend a reliable anti-rat c-kit (CD117) antibody that they have had good experience with on frozen sections? Thank you! Merced M Leiker Research Technician II Cardiovascular Medicine 361 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From cfarish <@t> csu.edu.au Wed Jun 3 18:40:17 2009 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Wed Jun 3 18:41:13 2009 Subject: [Histonet] TSH receptor In-Reply-To: References: <79A000B60AFE8045BA2581119DFEC444058A3F66@ESWW01.CSUMain.csu.edu.au> Message-ID: <79A000B60AFE8045BA2581119DFEC444058A4229@ESWW01.CSUMain.csu.edu.au> Thanks for the suggestion Bernice. I've been in contact with abcam (I maybe should have mentioned this). They have several TSH Receptor antibodies but unfortunately none are validated for IHC in sheep. They suggested a couple of possibilities based on cross-reactivity in pigs but obviously can make no definite recommendations. I though I'd see if anyone on histonet had any alternatives before I made any commitment. Cheers, Craig Craig (Joe)?Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW 2678 ? -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Wednesday, 3 June 2009 10:59 PM To: Farish, Craig Subject: RE: [Histonet] TSH receptor Craig, Have you tried abcam? www.abcam.com. You can filter by reactivity in species. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farish, Craig Sent: Tuesday, June 02, 2009 7:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TSH receptor Hi Folks, I'm looking for a TSH receptor antibody suitable for IHC in FFPE tissue from sheep. I'm struggling to find anything that may be applicable outside of the human field. Any suggestions would be most welcome. As always, thanks to all, Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW Australia "I don't want to achieve immortality through my work. I want to achieve immortality through not dying" Woody Allen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Jun 3 18:53:34 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 3 18:53:45 2009 Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding In-Reply-To: <14194B8417FC4AF79169F8D859D42826@stanford.edu> Message-ID: Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From nfournier <@t> sasktel.net Thu Jun 4 04:43:14 2009 From: nfournier <@t> sasktel.net (Neil M. Fournier) Date: Thu Jun 4 04:43:24 2009 Subject: [Histonet] sodium acetate and nickel-enhanced DAB Message-ID: <3A7EB9EDB3D74DA9A89A349406403104@Neil45EAF11E9E> I read somewhere that you are not to pH 0.175 M sodium acetate solution when using it to prepare a nickel-DAB chromogen solution. The problem is that whenever I make up 0.175 M sodium acetate (14.35 g of sodium acetate in 1 L of dH2O), the pH of my solution is generally around 8.0 to 8.1. From what I have read, the solution's pH should be closer to 7.0 (presumably w/o adjusting the pH of this solution)? Does anyone know what the correct pH of 0.175 M sodium acetate should be when preparing a nickel DAB chromogen solution? Am I missing something here? I appreciate the help Neil E-mail message checked by Spyware Doctor (6.0.1.441) Database version: 6.12540 http://www.pctools.com/en/spyware-doctor-antivirus/ From pattennj <@t> mail.nih.gov Thu Jun 4 08:54:58 2009 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Thu Jun 4 08:55:03 2009 Subject: [Histonet] Cresyl Violet Counterstain of LFB In-Reply-To: References: Message-ID: Hi Histonet... Quick question!! I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this? Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process? Thanks in advance! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health From rjbuesa <@t> yahoo.com Thu Jun 4 09:08:29 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 4 09:08:34 2009 Subject: [Histonet] Cresyl Violet Counterstain of LFB Message-ID: <821018.7087.qm@web65710.mail.ac4.yahoo.com> There is no problem with that. The whole idea of washing with ethanol is to differentiate and the section will be differentiated when no more color washes out. I don't know what artifacts you have but after sectioning there is nothing you can do. You can prevent the artifacts while sectioning and usually they are due to the time it takes to freeze the tissue and the freezing temp. also. Ren? J. --- On Thu, 6/4/09, Patten, Nicole (NIH/NIAAA) [F] wrote: From: Patten, Nicole (NIH/NIAAA) [F] Subject: [Histonet] Cresyl Violet Counterstain of LFB To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, June 4, 2009, 9:54 AM Hi Histonet... Quick question!! I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this? Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process? Thanks in advance! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From f.finlay <@t> formed.gla.ac.uk Thu Jun 4 09:30:35 2009 From: f.finlay <@t> formed.gla.ac.uk (Finlay Finlay) Date: Thu Jun 4 09:30:42 2009 Subject: [Histonet] obesity/cardiac study Message-ID: Hello histonet this is a call for any advice. I am a purely lab-taught histotech based in the UK and have been studying part-time to enable suitable accreditation and for my final year project am intending to use the large back catalogue of blocks in the forensic lab I work in to study cardiac changes associated with obesity (this is of special interest in the West of Scotland where I work). My intention is to select cases where obesity is judged (by pathologist) to be a primary or secondary factor in cause of death and where other factors (i.e. hypertension) were not indicated in life and compare with an age-matched cohort to asses the correlation between obesity and cardiac changes such as degree of interstitial fibrosis. Initially I intend to take a single case with well developed obesity-linked cardiac changes and do a range of stains (H&E, MSB, EVG, PTAH, a range of trichromes, PSR) to determine which stains to use in the whole study. I would be very grateful to hear from anyone who has undertaken similar work with advice on range of stains/techniques or tips on papers I should read or with any other advice. Also any advice on immuno that I could undertake. Thank you Finlay From gp62 <@t> georgetown.edu Thu Jun 4 09:46:54 2009 From: gp62 <@t> georgetown.edu (Guillermo Palchik) Date: Thu Jun 4 09:46:57 2009 Subject: [Histonet] Issues regarding flash freezing and cutting of Rat pup brains Message-ID: <282C42FB-DC79-4266-9B93-F7FD75588DC1@georgetown.edu> Dear Histoneters, I am looking for help regarding flash freezing of rat pup brains (Postnatal day 8). We need the tissue to be fresh (unfixed) for cutting at the cryostat. So far the technique has been: 1- to scoop the brains straight into cold (-50 C) Isopentane for about 10 seconds, 2 - dry for 10 seconds in dry ice, 3- wrap in tinfoil and into the -80 Freezer it goes. When it's time to cut, we apply a dab of OCT, flatten it with the heat extractor, apply another dab of OCT and stand the brain on the cerebellum (olfactory bulbs facing the person when cutting) and do a "ring" of OCT around the cerebellum (so actually most of the brain hits the cryostat blade directly (temp of cryostat ~ -20C). Mount on slides and put in the slide warmer (~ 37 - 40 C for 15 min). The slides then are all placed in a box and stored into a -20C freezer until processed for TUNEL (usually 3-5 days). As I said, the tissue does not look good (it curls, it cracks, etc..). I wanted to try mounting the whole brain into OCT and flash freeze the OCT block, and also even cryoprotecting the brains beforehand. I wanted to ask around if it is a good idea to cryoprotect (30% sucrose, O/N) since the brains are not fixed.... My "assumption" is that since the brain has not been fixed/perfused, upon bringing it up to room temperature, (or even 4C), enzymatic activity would resume and degrade the tissue, but I could be wrong... Any / other advice will be GREATLY appreciated, as well as pointing out gross mistakes on our part... Protocols also accepted! Thanks a lot. Gil Palchik gp62@georgetown.edu From dholmes <@t> anatomy.umsmed.edu Thu Jun 4 10:38:32 2009 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Thu Jun 4 10:38:54 2009 Subject: [Histonet] Microtome adjustment? Message-ID: <4A27A428020000820003ACCA@GWIA1.umsmed.edu> Thank everyone for all the great info and assistance in solving my problem. Paul, I am keeping you on my LIST for future repair needs. The problem turned out to be a 'fixable' one. One of our grad students had used the tome last week and adjusted the 'spring screws' behind the blade holder? This allowed the blade to move ever so slightly during the cutting process. I am sooo glad that I wont be losing my favorite piece of equipment, albeit an antique, just yet. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From mcauliff <@t> umdnj.edu Thu Jun 4 10:49:44 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 4 10:48:14 2009 Subject: [Histonet] Issues regarding flash freezing and cutting of Rat pup brains In-Reply-To: <282C42FB-DC79-4266-9B93-F7FD75588DC1@georgetown.edu> References: <282C42FB-DC79-4266-9B93-F7FD75588DC1@georgetown.edu> Message-ID: <4A27ED18.8060809@umdnj.edu> Just a few ideas: Do not cryoprotect unfixed tissue, it will just degrade. Do not immerse the brain directly in isopentane. Remove brain from pup, mount with a dab of OCT on chuck, put stem of chuck in -150 isopentane cooled with liquid N2, brain will freeze in 30-60 seconds.If your chucks are "stemless" find a round metal (brass, aluminum) rod to put the chuck on while the other end in in the isopentane. The brain may be drying out in the -80 freezer.You did not say how long the brains were stored before cutting. The brain is not equilibrating in the -20 cryostat from the -80 freezer, ie. the brain is too cold. We always wait 30 minutes or longer. Store sections at -80, -20 is not sufficient. Sharpen the knife and check the angle. Geoff Guillermo Palchik wrote: > Dear Histoneters, > > I am looking for help regarding flash freezing of rat pup brains > (Postnatal day 8). We need the tissue to be fresh (unfixed) for > cutting at the cryostat. > So far the technique has been: > > 1- to scoop the brains straight into cold (-50 C) Isopentane for > about 10 seconds, > 2 - dry for 10 seconds in dry ice, > 3- wrap in tinfoil and into the -80 Freezer it goes. > > When it's time to cut, we apply a dab of OCT, flatten it with the heat > extractor, apply another dab of OCT and stand the brain on the > cerebellum (olfactory bulbs facing the person when cutting) and do a > "ring" of OCT around the cerebellum (so actually most of the brain > hits the cryostat blade directly (temp of cryostat ~ -20C). Mount on > slides and put in the slide warmer (~ 37 - 40 C for 15 min). The > slides then are all placed in a box and stored into a -20C freezer > until processed for TUNEL (usually 3-5 days). As I said, the tissue > does not look good (it curls, it cracks, etc..). > > I wanted to try mounting the whole brain into OCT and flash freeze the > OCT block, and also even cryoprotecting the brains beforehand. I > wanted to ask around if it is a good idea to cryoprotect (30% sucrose, > O/N) since the brains are not fixed.... > My "assumption" is that since the brain has not been fixed/perfused, > upon bringing it up to room temperature, (or even 4C), enzymatic > activity would resume and degrade the tissue, but I could be wrong... > Any / other advice will be GREATLY appreciated, as well as pointing > out gross mistakes on our part... > Protocols also accepted! > > Thanks a lot. > > Gil Palchik > gp62@georgetown.edu > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From GoodwinD <@t> pahosp.com Thu Jun 4 11:10:47 2009 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Thu Jun 4 11:11:37 2009 Subject: [Histonet] Leprosy controls Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C46A@uphsmbx2.UPHS.PENNHEALTH.PRV> Greetings! Does anyone know of a source for M. leprae control slides? Tried both HCS and AMT - both are out of stock with no re-stocking date. Thanks! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Philadelphia, PA e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From MLunetta <@t> luhcares.org Thu Jun 4 13:15:54 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Jun 4 13:16:35 2009 Subject: [Histonet] Dako anti-bodies Message-ID: <4A27BAFA020000A800031BBC@mail.luhcares.org> Hello Netters We have just changed over to the new DAKO stainer and we have several anti-bodies RTU for the autostainer that we will not be using. All of the have a 2010 experation date. If you are interested in them please contact me off line AE1-3 2010-07 11ml BCL2 2010-06 11ml CD15 2009-09 11ml CD3 2010-07 11ml CD30 2010-01 11ml CD31 2010-05 7ml CD5 2010-03 11ml CEA 2010-08 11ml CK20 2010-07 7ml CK7 2010-07 7ml CMV 2010-06 7ml EMA 2010-06 11ml ER 2010-07 11ml ER 2010-07 11ml GFAP 2010-02 11ml HMB45 2010-04 11ml HMW 2010-06 11ml Ki67 2010-08 11ml LCA 2010-06 11ml MELAN A 2009-12 11ml PR 2010-07 11ml PSA 2009-05 11ml S100 2010-07 11ml SMA 2010-02 7ml SYNAPTP 2010-02 11ml TTF1 2010-06 11ml VIMEN 2010-04 11ml thanks, Matt Lunetta HT (ASCP) From GoodwinD <@t> pahosp.com Thu Jun 4 13:58:17 2009 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Thu Jun 4 13:59:10 2009 Subject: [Histonet] Leprosy controls In-Reply-To: <9ADE2F92328949E79B64AA45EDFF1864@lurie.northwestern.edu> References: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C46A@uphsmbx2.UPHS.PENNHEALTH.PRV> <9ADE2F92328949E79B64AA45EDFF1864@lurie.northwestern.edu> Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C470@uphsmbx2.UPHS.PENNHEALTH.PRV> FYI: Leptospira is a spirochete that causes leptospirosis in animals and can be transmitted to humans. Leprosy is caused by the organism Mycobacterium leprae, an acid fast organism best displayed by the Fite stain. Thanks to all for your replies. Diana Goodwin -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, June 04, 2009 2:45 PM To: Goodwin, Diana Subject: RE: [Histonet] Leprosy controls Newcomer has leptospira controls. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, Diana Sent: Thursday, June 04, 2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leprosy controls Greetings! Does anyone know of a source for M. leprae control slides? Tried both HCS and AMT - both are out of stock with no re-stocking date. Thanks! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Philadelphia, PA e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From ktuttle <@t> umm.edu Thu Jun 4 14:29:02 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Thu Jun 4 14:29:31 2009 Subject: [Histonet] Stat-Q Staining Kit Message-ID: <4A27E83D.90CE.001A.3@umm.edu> Does anyone use the Stat-Q Staining Kit by Innovex for multispecies IHC? Does it work as advertised? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From lldewe <@t> gmail.com Thu Jun 4 16:08:44 2009 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Thu Jun 4 16:08:50 2009 Subject: [Histonet] Stat-Q Staining Kit In-Reply-To: <4A27E83D.90CE.001A.3@umm.edu> References: <4A27E83D.90CE.001A.3@umm.edu> Message-ID: <7173d3c00906041408w7e87a01fnc2f68a3140300c64@mail.gmail.com> Yes, it absolutely does and more!! I have used it myself for many years in my vet histology and am completely pleased with it's performance!! Best Regards, Loralei Dewe On Thu, Jun 4, 2009 at 12:29 PM, Kimberly Tuttle wrote: > Does anyone use the Stat-Q Staining Kit by Innovex for multispecies IHC? > Does it work as advertised? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mwich <@t> 7thwavelabs.com Thu Jun 4 17:14:43 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Jun 4 17:14:55 2009 Subject: [Histonet] Stat-Q Staining Kit References: <4A27E83D.90CE.001A.3@umm.edu> Message-ID: <62A8156F8071C8439080D626DF8C33A65D8CA3@wave-mail.7thwave.local> We have also had great results with it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Thursday, June 04, 2009 2:29 PM To: histonet Subject: [Histonet] Stat-Q Staining Kit Does anyone use the Stat-Q Staining Kit by Innovex for multispecies IHC? Does it work as advertised? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From AnthonyH <@t> chw.edu.au Thu Jun 4 18:04:04 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 4 18:04:11 2009 Subject: [Histonet] Leprosy controls In-Reply-To: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C46A@uphsmbx2.UPHS.PENNHEALTH.PRV> Message-ID: Diana, Robert S. Richmond (in an old Histonet post) remarks that "leprosy requires control tissue containing the etiologic agent of the disease, Mycobacterium leprae, which has never been cultured. It is an acid-fast organism, but it does not have the same staining characteristics as Mycobacterium tuberculosis, and there is no substitute for it as a control. Leprosy commonly affects armadillos, and if a suitable human specimen is not available then infected armadillo liver is recommended." We don't have any armadillos in Australia, but I believe you should be able obtain some in the USA. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, Diana Sent: Friday, 5 June 2009 2:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leprosy controls Greetings! Does anyone know of a source for M. leprae control slides? Tried both HCS and AMT - both are out of stock with no re-stocking date. Thanks! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Philadelphia, PA e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mucram11 <@t> comcast.net Thu Jun 4 18:13:37 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Jun 4 18:13:43 2009 Subject: [Histonet] Stat-Q Staining Kit In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8CA3@wave-mail.7thwave.local> References: <4A27E83D.90CE.001A.3@umm.edu> <62A8156F8071C8439080D626DF8C33A65D8CA3@wave-mail.7thwave.local> Message-ID: <000901c9e56a$18773750$4965a5f0$@net> I answered off line however, we use Innovex Kits for animal tissue and it is the best we have found. Using it with Background Buster for routine work and the Fc Blocker for CNS tissue have given us reliable results we can depend on. Pam Marcum UPENN Sch of Vet Med New Bolton Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, June 04, 2009 6:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stat-Q Staining Kit We have also had great results with it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Thursday, June 04, 2009 2:29 PM To: histonet Subject: [Histonet] Stat-Q Staining Kit Does anyone use the Stat-Q Staining Kit by Innovex for multispecies IHC? Does it work as advertised? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Thu Jun 4 18:19:02 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Jun 4 18:19:07 2009 Subject: [Histonet] Cresyl Violet Counterstain of LFB Message-ID: <582736990906041619oe7f9335m3b69a675b2dfe23d@mail.gmail.com> Hi Nicole, Why bother dehydrating in ETOH then? Just rinse then air dry the sections. I had the same problem recently and this is what I do now to avoid the whole destaining problem. Once the slides are air dried, you can put them directly in xylene. Message: 12 Date: Thu, 4 Jun 2009 09:54:58 -0400 From: "Patten, Nicole (NIH/NIAAA) [F]" Subject: [Histonet] Cresyl Violet Counterstain of LFB To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonet... Quick question!! I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this? Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process? Thanks in advance! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health From AnthonyH <@t> chw.edu.au Thu Jun 4 18:21:37 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 4 18:21:52 2009 Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding In-Reply-To: <9D6C46390CA04C5482F2112664B0898C@stanford.edu> Message-ID: Nick, Well after 30 years of doing ICCs, the worst results are nearly always with tissues that have not been adequately fixed in formalin. This is based on using over 300 different antibodies in both adult and pediatric settings. The following paper shows an example of what can go wrong with under-fixed tissue: Gomes L, Mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge" J Histotechnol 31(4):138-184 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nicholas David Evans [mailto:ndevans@stanford.edu] Sent: Friday, 5 June 2009 8:56 AM To: Tony Henwood Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Thanks for the helpful response. After a consensus of views I decided to store at 70% ethanol for several days following 3-4hrs in 4% PFA. I think many people use paraffin embedded tissues for ICC and over-fixation is deleterious for this. 4% Paraformaldehyde (PFA) = 4g PFA dissolved in 100 mL PBS. Indeed when in solution it is present as formaldehyde. I will consider your suggestion of frozen sections if I have poor results. Thanks again for the e mail and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 03 June 2009 16:54 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From ndevans <@t> stanford.edu Thu Jun 4 18:40:13 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Thu Jun 4 18:40:18 2009 Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding In-Reply-To: References: <9D6C46390CA04C5482F2112664B0898C@stanford.edu> Message-ID: <6E356756FA1D4CE1A8B7FCD14BA80ED3@stanford.edu> Dear Tony and others, Thanks you very much and to others for the very helpful suggestions. I suppose there are many different ways to skin a cat! I will take on board all the valuable suggestions and use them to optimise my protocol. With thanks and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 04 June 2009 16:15 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Well after 30 years of doing ICCs, the worst results are nearly always with tissues that have not been adequately fixed in formalin. This is based on using over 300 different antibodies in both adult and pediatric settings. The accompanying paper shows an example of what can go wrong with under-fixed tissue: Gomes L, mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge" J Histotechnol 31(4):138-184 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nicholas David Evans [mailto:ndevans@stanford.edu] Sent: Friday, 5 June 2009 8:56 AM To: Tony Henwood Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Thanks for the helpful response. After a consensus of views I decided to store at 70% ethanol for several days following 3-4hrs in 4% PFA. I think many people use paraffin embedded tissues for ICC and over-fixation is deleterious for this. 4% Paraformaldehyde (PFA) = 4g PFA dissolved in 100 mL PBS. Indeed when in solution it is present as formaldehyde. I will consider your suggestion of frozen sections if I have poor results. Thanks again for the e mail and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 03 June 2009 16:54 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From dr.hatemsaied <@t> yahoo.com Thu Jun 4 22:01:50 2009 From: dr.hatemsaied <@t> yahoo.com (Hatem Salim) Date: Thu Jun 4 22:01:53 2009 Subject: [Histonet] immunohistochemistry Message-ID: <782588.78944.qm@web46103.mail.sp1.yahoo.com> HI ?I am Research assistant at Physiology department of Michigan state university. I am using the (?-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on mice femur sections. I have used this antigen unmasking method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. The problem is that bone detachment always occurs so I wonder if there is a method of retrieval that prevents bone detachment. > Waiting to hear from you soon. >? Thank you for your consideration > Best wishes > Hatem Salim From lpwenk <@t> sbcglobal.net Fri Jun 5 05:04:47 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 5 05:04:58 2009 Subject: [Histonet] Cresyl Violet Counterstain of LFB In-Reply-To: Message-ID: <31A0BF6B24EA460A9C5DD82980BCD64D@HPPav2> Some thoughts: 1. Are you using cresyl violet, or is it cresyl violet acetate, formerly known as cresyl echt violet. It should be the cresyl violet acetate. 2. 5 minutes may not be long enough. We use 1 hour. 3. After differentiation, the only things that should be violet are the nuclei of glial cells and neurons, and the nissl substance in the body of some neurons. And if you are doing the LFB first, the myelin changes from baby blue to medium blue. So there really won't be a lot of violet seen in some parts of brain, where there aren't a lot of neuron bodies. Just check the glial cell nuclei for positive staining. The following is our CEV (CVA), which can be used alone, like what follows. Or, skip step 1, and after you finish the LFB, go directly to step 2, and complete as written. CRESYL ECHT VIOLET 0.5 g Cresyl echt violet (Cresyl Violet Acetate) (no CI number) 0.18 g Sodium acetate (CH3COONaC3H2O) 500.0 mL Distilled water 1.5 mL Acetic acid, concentrated (CH3COOH)(approximately) Dissolve cresyl echt violet and sodium acetate in distilled water. Slowly add acetic acid, drop by drop, to solution. Should have a pH of 3.5. If solution pH is below 3.5, add more sodium acetate. If solution pH is above 3.5, add more acetic acid. Filter. Let stand overnight before using. Store at room temperature. Stable for months. May be reused until weak. PROCEDURE - Cresyl Echt Violet: 1. Deparaffinize and hydrate slides through graded alcohol to distilled water. 2. Place sections in cresyl echt violet solution 1 hour (can overstain, since differentiating out) 3. Differentiate in two changes of 95% ethanol until nuclei and Nissl granules remain violet and the background is nearly colorless. Check differentiation with the microscope. 1 to 2 drops of acetic acid many be added to the first alcohol to speed up differentiation. 4. Dehydrate through absolute ethanol and clear in xylene. 5. Coverslip with a synthetic mounting media. Lee & Peggy Wenk -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patten, Nicole (NIH/NIAAA) [F] Sent: Thursday, June 04, 2009 9:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cresyl Violet Counterstain of LFB Hi Histonet... Quick question!! I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this? Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process? Thanks in advance! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathleen.Caleri <@t> RoswellPark.org Fri Jun 5 06:46:28 2009 From: Kathleen.Caleri <@t> RoswellPark.org (Caleri, Kathleen) Date: Fri Jun 5 06:46:35 2009 Subject: [Histonet] Leica Peloris and ASP300 Message-ID: Hello-I would appreciate input and comments from anyone using the Leica Peloris or the ASP 300, Thanks! Regards, Kate Kathleen Caleri, BS, MLT, HT (ASCP) Histology Lab Supervisor Pathology Roswell Park Cancer Institute (716) 845-1329 "Weeds are flowers too, once you get to know them." -Eeyore This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From anh2006 <@t> med.cornell.edu Fri Jun 5 08:34:14 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Fri Jun 5 08:35:24 2009 Subject: [Histonet] immunohistochemistry In-Reply-To: <782588.78944.qm@web46103.mail.sp1.yahoo.com> References: <782588.78944.qm@web46103.mail.sp1.yahoo.com> Message-ID: <401579728-1244208915-cardhu_decombobulator_blackberry.rim.net-1656517679-@bxe1213.bisx.prod.on.blackberry> Does the marrow stay on but the cortical bone comes off? Are they paraffin sections? What is your decal method? Do you use coated slides? Do you "melt" your sections onto the slides before deparaffinization? Even so, I suspect the reason is your antigen retrieval solution is too hot and too vigorous. Lower the temp slightly and reduce the time. There is no need to boil for most antibodies. I do 95 deg C for 10 min with a 10 min cool down. Sometimes in super fresh sections cortical bone partially comes off, but marrow nd trabecular bone stays so I can get some information. Good luck! -----Original Message----- From: Hatem Salim Date: Thu, 04 Jun 2009 20:01:50 To: Subject: [Histonet] immunohistochemistry HI ?I am Research assistant at Physiology department of Michigan state university. I am using the (?-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on mice femur sections. I have used this antigen unmasking method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. The problem is that bone detachment always occurs so I wonder if there is a method of retrieval that prevents bone detachment. > Waiting to hear from you soon. >? Thank you for your consideration > Best wishes > Hatem Salim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Fri Jun 5 08:40:31 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Jun 5 08:40:37 2009 Subject: [Histonet] brilliant blue & safranin O Message-ID: Has anyone done this stain for chromatin and spindles in cell culture? My results in the search for this technique are very sketchy. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf From anh2006 <@t> med.cornell.edu Fri Jun 5 08:45:34 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Fri Jun 5 08:46:45 2009 Subject: [Histonet] Storage of tissues in PFA before dehydrationandembedding Message-ID: <870669244-1244209595-cardhu_decombobulator_blackberry.rim.net-224488157-@bxe1213.bisx.prod.on.blackberry> I would store in 30% ethanol instead of 70%. Mouse tissues are so brittle after dehydration and infiltration so keep the dehydration step in 70% EtOH the length of time indicated by your processor (usually 30 minutes or so). Also mouse skin is normally very thin, so if you fix for 4h at RT it should probably be sufficiently fixed - assuming you fix in sufficient volume not in a tiny tube (10-20 volumes excess). I go with the 1mm per hour rule of thumb for formalin/PFA fixation. You could also do overnight at 4 deg C. Either way be consistent across your study. As others have said, fixing longer (say 24-48 hours) will probably only help the morphology of tissues but may interfere with the staining with some antibodies - but that is also true for shorter fixation times and even the processing protocol itself. This is why we have antigen retrival! Good luck! From vetlab <@t> caribsurf.com Fri Jun 5 11:19:23 2009 From: vetlab <@t> caribsurf.com (Veterinary Services Laboratory) Date: Fri Jun 5 11:19:47 2009 Subject: [Histonet] Lab Water Quality Message-ID: I have one more question, what is the best electrical conductivity for lab water. We use softened reverse osmosis deionized water and consistently get an EC value of ~ 2. is this okay, if not, are their any suggestions. Thanks Sharon Ms. Sharon Drayton Veterinary Services Laboratory Ministry of Agriculture & Rural Development The Pine St. Michael BB11091 Barbados Tel: (246) 427-5492 or (246) 427-5073 Fax: (246)426-7517 Email: vetlab@caribsurf.com Website: www.agriculture.gov.bb From leiker <@t> buffalo.edu Fri Jun 5 11:34:03 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Jun 5 11:34:09 2009 Subject: [Histonet] Lab Water Quality In-Reply-To: References: Message-ID: <29B1A826E1CC89410ED43EE1@CDYwxp1931.ad.med.buffalo.edu> 18.2 mega-ohms of electrical resistance. --On Friday, June 05, 2009 12:19 PM -0400 Veterinary Services Laboratory wrote: > I have one more question, what is the best electrical conductivity for lab > water. We use softened reverse osmosis deionized water and consistently > get an EC value of ~ 2. is this okay, if not, are their any suggestions. > > > > > > Thanks Sharon > > > > Ms. Sharon Drayton > > Veterinary Services Laboratory > > Ministry of Agriculture & Rural Development > > The Pine > > St. Michael BB11091 > > Barbados > > > > Tel: (246) 427-5492 or (246) 427-5073 > > Fax: (246)426-7517 > > Email: vetlab@caribsurf.com > > Website: www.agriculture.gov.bb > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 361 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Heather.D.Renko <@t> osfhealthcare.org Fri Jun 5 12:40:25 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Jun 5 12:41:07 2009 Subject: [Histonet] re: Cut off times for tissue to be processed Message-ID: I am trying to develop a policy for cut off times for putting tissue into the processor and wanted find out if anyone out there would like to share what they have. Please email me directly-thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Chris.Edwards <@t> nationwidechildrens.org Fri Jun 5 13:15:58 2009 From: Chris.Edwards <@t> nationwidechildrens.org (Edwards, Chris) Date: Fri Jun 5 13:16:23 2009 Subject: [Histonet] Toluidine blue staining Message-ID: Hello. Our lab has had highly variable results using toluidine blue to stain 1.5 micron thick cross sections of plastic embedded nerve tissue from both mouse and human nerve biopsy. It seems like the stain is darker in the biopsy tissue than the mouse but both sometimes stain equally poorly. We have tried changing the protocol recipe to make it darker by adding more toluidine blue powder but that has not worked either. We have used 2 grams toluidine blue, and 2 grams of sodium borax with 100 ml of distilled water. We have tried changing the sodium borax. Sodium tetraborate-decahydrate and a sodium tertaborate 99.9% metals basis from sigma Aldrich have both had the same results. Does anyone have any idea how to make it stain darker? I can't really emphasize enough how light the blue is. I did read one protocol that involved adjusting pH, is that a possible factor? Thanks Christopher J. Edwards Research Associate, Sahenk Lab Center for Gene Therapy Wexner Building 2, Room 3224 Lab Phone: 355-5224 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Rcartun <@t> harthosp.org Fri Jun 5 13:43:34 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 5 13:43:47 2009 Subject: [Histonet] re: Cut off times for tissue to be processed In-Reply-To: References: Message-ID: <4A292F16.7400.0077.1@harthosp.org> We try to start our tissue processors at 5:30 p.m. every night (our processors for large specimens have 4 hours of formalin on them). I say "try" because sometimes residents or fellows bring late specimens down to our grossing room from the OR where they have already been grossed which results in starting the machine(s) later. Therefore, we have a 3:30 p.m. cut-off time for large specimens (breast, colon, prostate, etc.) to ensure they get at least 6 hours of formalin fixation. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Renko, Heather D." 6/5/2009 1:40 PM >>> I am trying to develop a policy for cut off times for putting tissue into the processor and wanted find out if anyone out there would like to share what they have. Please email me directly-thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Jun 5 13:49:30 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 5 13:47:59 2009 Subject: [Histonet] Toluidine blue staining In-Reply-To: References: Message-ID: <4A2968BA.7010300@umdnj.edu> To get consistent staining you must standardize your procedure. I make 1% aqueous Tol. Blue and 2% aqueous borax (sodium tetraborate) in separate containers. Each day I mix equal parts of each solution so I always use fresh stain. My final conc. of stain is 0.5%, doubling to 1% makes no difference. Stain at consistent temperature in a water bath (60-65 degrees C) for a consistent time. Not dark enough? Stain longer and/or at a higher temp. When I stopped using a drop (how big?) of stain on a hotplate (how hot?) until I though it was stained (how long?) I got much better results. Also, are the biopsy and the mouse tissue fixed and processed identically? Geoff Edwards, Chris wrote: > Hello. Our lab has had highly variable results using toluidine blue to > stain 1.5 micron thick cross sections of plastic embedded nerve tissue > from both mouse and human nerve biopsy. It seems like the stain is > darker in the biopsy tissue than the mouse but both sometimes stain > equally poorly. We have tried changing the protocol recipe to make it > darker by adding more toluidine blue powder but that has not worked > either. We have used 2 grams toluidine blue, and 2 grams of sodium borax > with 100 ml of distilled water. We have tried changing the sodium borax. > Sodium tetraborate-decahydrate and a sodium tertaborate 99.9% metals > basis from sigma Aldrich have both had the same results. Does anyone > have any idea how to make it stain darker? I can't really emphasize > enough how light the blue is. I did read one protocol that involved > adjusting pH, is that a possible factor? Thanks > > > > > > > > > > > > > > > > > > > > > > > > Christopher J. Edwards > > Research Associate, Sahenk Lab > > Center for Gene Therapy > > Wexner Building 2, Room 3224 > > Lab Phone: 355-5224 > > > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From gayle.callis <@t> bresnan.net Fri Jun 5 14:41:01 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jun 5 14:41:17 2009 Subject: [Histonet] Re: Toludine blue stain Message-ID: <000001c9e615$8fc16e10$af444a30$@callis@bresnan.net> You mention plastic? Which one are you using? I have more than one toluidine blue staining method depending on the plastic I am using. The T blue/sodium borate was used mostly for EM resins, but not for methyl or glycol methacrylates. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT From pattennj <@t> mail.nih.gov Fri Jun 5 14:46:36 2009 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Fri Jun 5 14:46:43 2009 Subject: [Histonet] Frozen Artifacts Message-ID: Hello Histonet... I am using frozen brain sections and I have terrible frozen artifacts. I receive the tissue from a brain bank so I have no control over the method of freezing. Is there ANYTHING that I can do at this point to at least reduce these artifacts? I've tried formalin fixation post-cutting and reducing the temperature of the cryostat but nothing works.... the others in my lab also have no idea. Any help would be GREATLY appreciated. Thanks!! -Nicole From j.aflleje <@t> integrated-pathology.com Fri Jun 5 15:39:49 2009 From: j.aflleje <@t> integrated-pathology.com (James Aflleje) Date: Fri Jun 5 15:39:55 2009 Subject: [Histonet] off e-mail list new account Message-ID: <1BEBBEAB73EB8F40B2CEF99ACD5B33A7AA6757B0EF@Exchange.IPath.local> Webmaster please be kind to take me off your e-mail address. Thanks, James Aflleje HTL ASCP, Director of Laboratory, INTEGRATED-PATHOLOGY From carrolpb <@t> umdnj.edu Fri Jun 5 15:43:12 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jun 5 15:43:23 2009 Subject: [Histonet] off e-mail list new account In-Reply-To: <1BEBBEAB73EB8F40B2CEF99ACD5B33A7AA6757B0EF@Exchange.IPath.local> References: <1BEBBEAB73EB8F40B2CEF99ACD5B33A7AA6757B0EF@Exchange.IPath.local> Message-ID: <4A298360.6010206@umdnj.edu> Webmaster, please be kind enough to serve us all tea and snacks, too. It's Friday afternoon! ;) James Aflleje wrote: > Webmaster please be kind to take me off your e-mail address. > > > Thanks, > > > James Aflleje > HTL ASCP, > Director of Laboratory, > INTEGRATED-PATHOLOGY > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From vapatpxs <@t> yahoo.com Fri Jun 5 15:50:12 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Fri Jun 5 15:50:17 2009 Subject: [Histonet] Frozen Artifacts Message-ID: <925234.10727.qm@web46111.mail.sp1.yahoo.com> Hi Nicole, You must tell the list what the artifacts are before we can help you. You're note is a little too vague. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Fri, 6/5/09, Patten, Nicole (NIH/NIAAA) [F] wrote: From: Patten, Nicole (NIH/NIAAA) [F] Subject: [Histonet] Frozen Artifacts To: "histonet@lists.utsouthwestern.edu" Cc: "Rodriguez-canales, Jaime (NIH/NCI) [F]" Date: Friday, June 5, 2009, 7:46 PM Hello Histonet... I am using frozen brain sections and I have terrible frozen artifacts. I receive the tissue from a brain bank so I have no control over the method of freezing. Is there ANYTHING that I can do at this point to at least reduce these artifacts? I've tried formalin fixation post-cutting and reducing the temperature of the cryostat but nothing works.... the others in my lab also have no idea. Any help would be GREATLY appreciated. Thanks!! -Nicole _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Jun 5 16:03:07 2009 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Jun 5 16:07:40 2009 Subject: [Histonet] immunohistochemistry Message-ID: <37DEF9AF72994947AF693956A59B9B66019A68D5@mailbe03.mc.vanderbilt.edu> I would recommend the following: Citrate for 35 min at 95 deg. Cool down for 10 min. (This is our protocol.) Be sure you are using charged slides and I would let them air dry for at least 1 hour before heating and deparaffinizing. That might help, too. Unfortunately, I don't think there is an effective method of retrieival that will completely eliminate the possibilty of floating tissue. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT) From: Hatem Salim Subject: [Histonet] immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <782588.78944.qm@web46103.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 HI I am Research assistant at Physiology department of Michigan state university. I am using the (?-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on mice femur sections. I have used this antigen unmasking method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. The problem is that bone detachment always occurs so I wonder if there is a method of retrieval that prevents bone detachment. > Waiting to hear from you soon. > Thank you for your consideration > Best wishes > Hatem Salim From gayle.callis <@t> bresnan.net Fri Jun 5 16:27:30 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jun 5 16:27:46 2009 Subject: [Histonet] immunohistochemistry In-Reply-To: <37DEF9AF72994947AF693956A59B9B66019A68D5@mailbe03.mc.vanderbilt.edu> References: <37DEF9AF72994947AF693956A59B9B66019A68D5@mailbe03.mc.vanderbilt.edu> Message-ID: <001801c9e624$6fe4da50$4fae8ef0$@callis@bresnan.net> Good advice since actively boiling buffer puts mechanical stresses on section, with bone more easily loosened by this action. It may help to preheat the buffer to 95C before slide/section immersion into retrieval buffer. A friend who is an IHC expert always did this, plus she used plastic coplin jars (safer!). Also, we dry all our decalcified, paraffin embedded bone slides FLAT, at 37C to 40C for overnight, with longer better - be sure slides are well drained before laying them flat. You can go to a 56C paraffin oven for a short time (just enough to melt paraffin) after overnight if you desire, but you find that unnecessary. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Friday, June 05, 2009 3:03 PM To: Histonet Subject: Re: [Histonet] immunohistochemistry I would recommend the following: Citrate for 35 min at 95 deg. Cool down for 10 min. (This is our protocol.) Be sure you are using charged slides and I would let them air dry for at least 1 hour before heating and deparaffinizing. That might help, too. Unfortunately, I don't think there is an effective method of retrieival that will completely eliminate the possibilty of floating tissue. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT) From: Hatem Salim Subject: [Histonet] immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <782588.78944.qm@web46103.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 HI I am Research assistant at Physiology department of Michigan state university. I am using the (?-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on mice femur sections. I have used this antigen unmasking method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. The problem is that bone detachment always occurs so I wonder if there is a method of retrieval that prevents bone detachment. > Waiting to hear from you soon. > Thank you for your consideration > Best wishes > Hatem Salim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From burch007 <@t> mc.duke.edu Fri Jun 5 17:12:33 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Jun 5 17:12:42 2009 Subject: [Histonet] immunohistochemistry In-Reply-To: <37DEF9AF72994947AF693956A59B9B66019A68D5@mailbe03.mc.vanderbilt.edu> References: <37DEF9AF72994947AF693956A59B9B66019A68D5@mailbe03.mc.vanderbilt.edu> Message-ID: I second Mr. Troutman's recommendation using silane slides, dry overnight. Once the slides have been baked and paraffin re place the slides in citrate buffer and heat them in a closed plastic coplin jar for 12 hours at 75 degrees C. Some epitopes can be retrieved wi Jim Burchette, H "A simple histotech from a little country hospital in North From talulahgosh <@t> gmail.com Fri Jun 5 21:32:22 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 5 21:32:29 2009 Subject: [Histonet] off e-mail list new account In-Reply-To: <4A298360.6010206@umdnj.edu> References: <1BEBBEAB73EB8F40B2CEF99ACD5B33A7AA6757B0EF@Exchange.IPath.local> <4A298360.6010206@umdnj.edu> Message-ID: Webmaster! A beer! "Canned food is dead food....here kids can't study. They're going blind. Mothers are out in pants showing themselves off when they ought to be at home cooking fresh vegetables." --Charles Atlas, Saga magazine interview, 1964 On Fri, Jun 5, 2009 at 4:43 PM, Peter Carroll wrote: > Webmaster, please be kind enough to serve us all tea and snacks, too. It's > Friday afternoon! ;) > > > James Aflleje wrote: > >> Webmaster please be kind to take me off your e-mail address. >> >> >> Thanks, >> >> >> James Aflleje >> HTL ASCP, >> Director of Laboratory, >> INTEGRATED-PATHOLOGY >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jmeade0710 <@t> aol.com Fri Jun 5 23:44:32 2009 From: jmeade0710 <@t> aol.com (Jerry Meade) Date: Fri Jun 5 22:57:37 2009 Subject: [Histonet] Jerry sent you photos on Tagged :) Message-ID: <967859024-294126278@pathology.swmed.edu> [1]If you can't see this email please click here [imgsrv.php?uid=5423200617&ect=1hpm385b] Jerry Meade Jerry Meade sent you photos on Tagged Want to see the photos? Click Yes if you want to see the photos, otherwise click No. But you have to click! Please respond or Jerry may think you said no :( [2]Click here to block all emails from Tagged Inc., 110 Pacific Mall Box #117, San Francisco, CA. 94111 References Visible links 1. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm385b&tId=150337&fid=7f7a1b535d8d1dd1&emt=1000&ict=0&linkId=0 2. http://www.taggedmail.com/no_more.html?unsem=histonet%40pathology.swmed.edu&tId=150337&fid=7f7a1b535d8d1dd1&linkId=3 Hidden links: 3. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm385b&tId=150337&fid=7f7a1b535d8d1dd1&emt=1000&ict=0&bn=1&linkId=1 4. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm385b&tId=150337&fid=7f7a1b535d8d1dd1&emt=1000&ict=0&bn=2&linkId=2 From jmeade0710 <@t> aol.com Fri Jun 5 23:45:25 2009 From: jmeade0710 <@t> aol.com (Jerry Meade) Date: Fri Jun 5 22:58:30 2009 Subject: [Histonet] Jerry sent you a private message on Tagged :) Message-ID: <967858971-294129452@pathology.swmed.edu> [1]If you can't see this email please click here [imgsrv.php?uid=5423200617&ect=1hpm9sxh] Jerry Meade Jerry Meade has added you as a friend Do you know Jerry? Please click Yes or No :) Please respond in the next 3 minutes! [2]Click here to block all emails from Tagged Inc., 110 Pacific Mall Box #117, San Francisco, CA. 94111 References Visible links 1. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm9sxh&tId=150010&fid=7f7a1b535d8d1dd1&emt=1002&ict=0&linkId=0 2. http://www.taggedmail.com/no_more.html?unsem=histonet%40pathology.swmed.edu&tId=150010&fid=7f7a1b535d8d1dd1&linkId=3 Hidden links: 3. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm9sxh&tId=150010&fid=7f7a1b535d8d1dd1&emt=1002&ict=0&bn=1&linkId=1 4. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm9sxh&tId=150010&fid=7f7a1b535d8d1dd1&emt=1002&ict=0&bn=2&linkId=2 From pruegg <@t> ihctech.net Sun Jun 7 11:01:17 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jun 7 11:01:26 2009 Subject: [Histonet] immunohistochemistry In-Reply-To: <001801c9e624$6fe4da50$4fae8ef0$@callis@bresnan.net> References: <37DEF9AF72994947AF693956A59B9B66019A68D5@mailbe03.mc.vanderbilt.edu> <001801c9e624$6fe4da50$4fae8ef0$@callis@bresnan.net> Message-ID: I have done extensive IHC work on bone and cartilage samples and in my experience, controlled enzyme digestion such as pepsin at 37dc flat on a slide warmer, or trypsin, or proteinase K are my first choices for AR on bone samples. Complete draining and airdrying is a must before heating the slides, I, like Gayle try to airdry overnight after completely draining the water by standing the slides up for at least an hour. Drying them flat on a slide warmer at 37-40dc is suggested. I do heat them on the same flat slide warmer (mine goes up to only 55dc) to melt the paraffin for maybe 30 min or so. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Friday, June 05, 2009 3:28 PM To: 'Troutman, Kenneth A'; 'Histonet' Subject: RE: [Histonet] immunohistochemistry Good advice since actively boiling buffer puts mechanical stresses on section, with bone more easily loosened by this action. It may help to preheat the buffer to 95C before slide/section immersion into retrieval buffer. A friend who is an IHC expert always did this, plus she used plastic coplin jars (safer!). Also, we dry all our decalcified, paraffin embedded bone slides FLAT, at 37C to 40C for overnight, with longer better - be sure slides are well drained before laying them flat. You can go to a 56C paraffin oven for a short time (just enough to melt paraffin) after overnight if you desire, but you find that unnecessary. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Friday, June 05, 2009 3:03 PM To: Histonet Subject: Re: [Histonet] immunohistochemistry I would recommend the following: Citrate for 35 min at 95 deg. Cool down for 10 min. (This is our protocol.) Be sure you are using charged slides and I would let them air dry for at least 1 hour before heating and deparaffinizing. That might help, too. Unfortunately, I don't think there is an effective method of retrieival that will completely eliminate the possibilty of floating tissue. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT) From: Hatem Salim Subject: [Histonet] immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <782588.78944.qm@web46103.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 HI I am Research assistant at Physiology department of Michigan state university. I am using the (?-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on mice femur sections. I have used this antigen unmasking method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. The problem is that bone detachment always occurs so I wonder if there is a method of retrieval that prevents bone detachment. > Waiting to hear from you soon. > Thank you for your consideration > Best wishes > Hatem Salim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jun 7 11:10:27 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jun 7 11:10:33 2009 Subject: [Histonet] Storage of tissues in PFA before dehydrationandembedding In-Reply-To: References: <9D6C46390CA04C5482F2112664B0898C@stanford.edu> Message-ID: <0C27E335F7144959BF0BAE5F6F2B9C7B@prueggihctechlt> I agree with Tony, the problem is usually underfixation not over with aldehyde fixatives, if you do not stabilize the proteins by fixing (Bryan Hewlett says this takes 24 hrs. no matter the size of the tissue) paraffin processing will adversely affect your tissue and in some cases the proteins of interest will be washed away and lost with no change or no amount of AR to get them back. Researchers are stuck in the days before AR techniques when cross linking of proteins by aldehyde fixation was a problem because we did not know how to retrieve them, but now we do, and the bigger problem is losing antigens from paraffin processing because they have not been adequately fixed to protect them. I recommend 24-72 hour fixation in aldehyde fixative and then the tissues can be placed in 70% alcohol. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, June 04, 2009 5:22 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydrationandembedding Nick, Well after 30 years of doing ICCs, the worst results are nearly always with tissues that have not been adequately fixed in formalin. This is based on using over 300 different antibodies in both adult and pediatric settings. The following paper shows an example of what can go wrong with under-fixed tissue: Gomes L, Mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge" J Histotechnol 31(4):138-184 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nicholas David Evans [mailto:ndevans@stanford.edu] Sent: Friday, 5 June 2009 8:56 AM To: Tony Henwood Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Thanks for the helpful response. After a consensus of views I decided to store at 70% ethanol for several days following 3-4hrs in 4% PFA. I think many people use paraffin embedded tissues for ICC and over-fixation is deleterious for this. 4% Paraformaldehyde (PFA) = 4g PFA dissolved in 100 mL PBS. Indeed when in solution it is present as formaldehyde. I will consider your suggestion of frozen sections if I have poor results. Thanks again for the e mail and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 03 June 2009 16:54 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. 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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jun 7 11:12:17 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jun 7 11:12:21 2009 Subject: FW: [Histonet] coverslippers Message-ID: <9DFD9F08C7A74FF8B342ADE00ACBE785@prueggihctechlt> I didn't get very many response to this, so I will post again. Perhaps not many have used this coverslipper? I am not sure of how well it sold when SP distributed it? Patsy I am in the process of helping to set up a new histology lab and we want to get a stand alone glass coverslipper. I know that this is one piece of equipment that can be really finicky. SurgiPath used to market a small glass coverslipper which has now been bought by Dako. Are there any of you out there that use or used this coverslipper when SuriPath sold it? I am wondering how this coverslipper performed in the field? Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 <@t> gmail.com Sun Jun 7 12:18:29 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Sun Jun 7 12:18:35 2009 Subject: [Histonet] immunohistochemistry Message-ID: Hatem: Since this info hasn't already been offered, I'd suggest that you considering using a 'programmable' water bath or pressure cooker -- where the temperature is reduced to 65 to 75 degrees, but the exposure time extended to three to six hours. Sally ----------------------------------- Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT) From: Hatem Salim Subject: [Histonet] immunohistochemistry To: histonet@lists.utsouthwestern.edu HI I am Research assistant at Physiology department of Michigan state university. I am using the (?-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on mice femur sections. I have used this antigen unmasking method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. The problem is that bone detachment always occurs so I wonder if there is a method of retrieval that prevents bone detachment. Waiting to hear from you soon. Thank you for your consideration Best wishes Hatem Salim From sjromey <@t> bellsouth.net Sun Jun 7 14:40:04 2009 From: sjromey <@t> bellsouth.net (Shirley Romey) Date: Sun Jun 7 15:40:09 2009 Subject: Fw: [Histonet] Jerry sent you a private message on Tagged :) Message-ID: <684579.34285.qm@web180604.mail.sp1.yahoo.com> Not sure how this got through histonet!!!? This is not the first time something like this has happened.... ----- Forwarded Message ---- From: Jerry Meade To: histonet@pathology.swmed.edu Sent: Saturday, June 6, 2009 12:45:25 AM Subject: [Histonet] Jerry sent you a private message on Tagged :) ? [1]If you can't see this email please click here ? [imgsrv.php?uid=5423200617&ect=1hpm9sxh] ? Jerry Meade ? Jerry Meade has added you as a friend ? Do you know Jerry? ? Please click Yes or No :) ? Please respond in the next 3 minutes! ? [2]Click? here? to block all emails from Tagged Inc., 110 Pacific Mall ? Box #117, San Francisco, CA. 94111 References ? Visible links ? 1. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm9sxh&tId=150010&fid=7f7a1b535d8d1dd1&emt=1002&ict=0&linkId=0 ? 2. http://www.taggedmail.com/no_more.html?unsem=histonet%40pathology.swmed.edu&tId=150010&fid=7f7a1b535d8d1dd1&linkId=3 ? Hidden links: ? 3. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm9sxh&tId=150010&fid=7f7a1b535d8d1dd1&emt=1002&ict=0&bn=1&linkId=1 ? 4. http://www.taggedmail.com/welcome.html?conn=2japa8jk4&ect=1hpm9sxh&tId=150010&fid=7f7a1b535d8d1dd1&emt=1002&ict=0&bn=2&linkId=2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Sun Jun 7 21:49:57 2009 From: tifei <@t> foxmail.com (TF) Date: Sun Jun 7 21:50:43 2009 Subject: [Histonet] Storage of tissues in PFA beforedehydrationandembedding References: <9D6C46390CA04C5482F2112664B0898C@stanford.edu>, , <0C27E335F7144959BF0BAE5F6F2B9C7B@prueggihctechlt> Message-ID: <200906081049524527524@foxmail.com> SnVzdCB3YW50IHRvIG1lbnRpb24gYSBwb2ludCB0aGF0IHNvbWUgYW50aWdlbnMgYXJlIHRvbyBm cmFnaWxlIHRvIFBGQSBhbmQgaW4gbXkgaGFuZCBjYW4gbm90IGJlIHJldHJpZXZhbCJlZCIgYW55 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dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQpfX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGluZyBsaXN0DQpI aXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNvdXRod2Vz dGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K From anand <@t> ethoneuro.com Sun Jun 7 23:07:21 2009 From: anand <@t> ethoneuro.com (Anand Vasudevan) Date: Sun Jun 7 23:07:28 2009 Subject: [Histonet] MAP2 and GFAP double staining Message-ID: <189449020906072107m396be1e1s64b99df46e16167e@mail.gmail.com> Hi, I would like to know if anyone has carried out MAP2 and GFAP double staining? If so, I would appreciate if you could the antibodies used and the detection method? Thanks, Anand Vasudevan Nanyang Technological University (NTU) Singapore From anh2006 <@t> med.cornell.edu Mon Jun 8 07:37:57 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Mon Jun 8 07:39:06 2009 Subject: [Histonet] Storage of tissues in PFAbeforedehydrationandembedding In-Reply-To: <200906081049524527524@foxmail.com> References: <9D6C46390CA04C5482F2112664B0898C@stanford.edu><0C27E335F7144959BF0BAE5F6F2B9C7B@prueggihctechlt><200906081049524527524@foxmail.com> Message-ID: <265840770-1244464739-cardhu_decombobulator_blackberry.rim.net-1060202763-@bxe1213.bisx.prod.on.blackberry> It all depends on the antibody and clone and the processing (not only the fixation) - even for CD31. -----Original Message----- From: TF Date: Mon, 08 Jun 2009 10:49:57 To: Patsy Ruegg; 'Tony Henwood'; histonet@lists.utsouthwestern.edu Subject: Re: RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding Just want to mention a point that some antigens are too fragile to PFA and in my hand can not be retrieval"ed" anymore...such as rat CD31. 2009-06-08 TF ???? Patsy Ruegg ????? 2009-06-08 00:13:46 ???? 'Tony Henwood'; histonet@lists.utsouthwestern.edu ??? ??? RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding I agree with Tony, the problem is usually underfixation not over with aldehyde fixatives, if you do not stabilize the proteins by fixing (Bryan Hewlett says this takes 24 hrs. no matter the size of the tissue) paraffin processing will adversely affect your tissue and in some cases the proteins of interest will be washed away and lost with no change or no amount of AR to get them back. Researchers are stuck in the days before AR techniques when cross linking of proteins by aldehyde fixation was a problem because we did not know how to retrieve them, but now we do, and the bigger problem is losing antigens from paraffin processing because they have not been adequately fixed to protect them. I recommend 24-72 hour fixation in aldehyde fixative and then the tissues can be placed in 70% alcohol. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, June 04, 2009 5:22 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydrationandembedding Nick, Well after 30 years of doing ICCs, the worst results are nearly always with tissues that have not been adequately fixed in formalin. This is based on using over 300 different antibodies in both adult and pediatric settings. The following paper shows an example of what can go wrong with under-fixed tissue: Gomes L, Mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge" J Histotechnol 31(4):138-184 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nicholas David Evans [mailto:ndevans@stanford.edu] Sent: Friday, 5 June 2009 8:56 AM To: Tony Henwood Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Thanks for the helpful response. After a consensus of views I decided to store at 70% ethanol for several days following 3-4hrs in 4% PFA. I think many people use paraffin embedded tissues for ICC and over-fixation is deleterious for this. 4% Paraformaldehyde (PFA) = 4g PFA dissolved in 100 mL PBS. Indeed when in solution it is present as formaldehyde. I will consider your suggestion of frozen sections if I have poor results. Thanks again for the e mail and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 03 June 2009 16:54 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Mon Jun 8 08:19:39 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Jun 8 08:19:44 2009 Subject: [Histonet] Lymphocytes IHC Message-ID: <35e16a770906080619x502cd9b0pf13e352a4ab1f0fa@mail.gmail.com> Dear Histonetters! I am wondering whether there is a universal IHC marker for different types of lymphocytes, both B and T??? I am trying to start working on inflammation models and have no previous experience with inflammation markers. If anyone knows any, that would be great if they work on paraffin embedded tissues since most CD markers work on frozen only (it's for archival samples). Thank you very much in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 From joseph-galbraith <@t> uiowa.edu Mon Jun 8 08:34:05 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Jun 8 08:34:17 2009 Subject: [Histonet] Lymphocytes IHC In-Reply-To: <35e16a770906080619x502cd9b0pf13e352a4ab1f0fa@mail.gmail.com> References: <35e16a770906080619x502cd9b0pf13e352a4ab1f0fa@mail.gmail.com> Message-ID: Igor: CD45 is a general marker for cells of hematopoietic origin and will stain T, B and NK subsets of lymphocytes but not plasma cells. CD19 or CD20 will stain most B-cells and CD3 will stain most T-cells. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Monday, June 08, 2009 8:20 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lymphocytes IHC Dear Histonetters! I am wondering whether there is a universal IHC marker for different types of lymphocytes, both B and T??? I am trying to start working on inflammation models and have no previous experience with inflammation markers. If anyone knows any, that would be great if they work on paraffin embedded tissues since most CD markers work on frozen only (it's for archival samples). Thank you very much in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Mon Jun 8 09:23:18 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Mon Jun 8 09:23:26 2009 Subject: [Histonet] Chris - T.Blue/nerve tissue Message-ID: <00ad01c9e844$ab5822f0$c5d76880@vetmed.wisc.edu> Chris, If you do not do this, consider etching your tissue to remove the surface plastic. We use 0.2% formic acid - start with less than a minute as your tissues are thin. Some labs etch using ethyl alcohol or methanol. We have success and good contrast with a pH less than 5. For thinner sections, I have used a T.Blue 0.1 % Aq for Metachromasia from the PolyScientific Co. Rinse well with water and dip into 90% at the end ( give great contrast). Vicki From Pat.Patterson <@t> propath.com Mon Jun 8 09:46:24 2009 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Mon Jun 8 09:46:29 2009 Subject: [Histonet] Immunohistochemistry Tech - Dallas Message-ID: <82C7248978CB50469FD6BA68EBBEFE6701A3AD12@exchange.propathlab.com> We have an opening in our IHC Lab - contact HR or myself with questions. ProPath, a progressive, CAP accredited, high-volume pathology lab in Dallas has a full-time opportunity for an Immunohistochemistry Technician. Responsibilities include: slide preparation (paraffin and frozen sections), manual IHC staining, antibody titer preparation, equipment maintenance, supply and reagent inventory maintenance and QC/QA record maintenance. HT (ASCP) or QIHC registered or eligible. A minimum of 2 years of histology experience required. ProPath utilizes technology and is a quality oriented pathology laboratory. Competitive salary with benefits offered. Medical, dental, company paid Short Term and Long Term disability insurance, a matched 401K plan and more! For consideration, send resume to: Human Resources ProPath 8267 Elmbrook Drive Suite 100 Dallas, TX 75247. FAX: 214-237-1825 Job Line 214-237-1775 Email address: jobs@propath.com Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 8267 Elmbrook Drive; Suite 100 Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com From rjbuesa <@t> yahoo.com Mon Jun 8 09:51:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 8 09:51:05 2009 Subject: [Histonet] Lymphocytes IHC Message-ID: <272514.19064.qm@web65715.mail.ac4.yahoo.com> Igor: The list of CD markers that work on FFPE tissues is quite extensive. I think that you will better off if you contact a Leica Microsystems representative and ask for the Novocastra catalog (that they own now) so you can have an idea of the wide arsenal of CD markers available for paraffin embedded tissues. Ren? J. --- On Mon, 6/8/09, Igor Deyneko wrote: From: Igor Deyneko Subject: [Histonet] Lymphocytes IHC To: Histonet@lists.utsouthwestern.edu Date: Monday, June 8, 2009, 9:19 AM Dear Histonetters! I am wondering whether there is a universal IHC marker for different types of lymphocytes, both B and T??? I am trying to start working on inflammation models and have no previous experience with inflammation markers. If anyone knows any, that would be great if they work on paraffin embedded tissues since most CD markers work on frozen only (it's for archival samples). Thank you very much in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Mon Jun 8 10:56:49 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Jun 8 10:57:42 2009 Subject: [Histonet] Modified Davidson's fixative use Message-ID: Dear colleagues, For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol? Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Jackie.O'Connor <@t> abbott.com Mon Jun 8 12:06:13 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Jun 8 12:06:36 2009 Subject: [Histonet] Modified Davidson's fixative use In-Reply-To: Message-ID: To not answer your question - we fix eyes and testes in Modified Davidsons for 48 hours then transfer to formalin to await processing. Since mDF is basically formalin, alcohol, and gaa - a water rinse step is pretty moot. "Johnson, Teri" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2009 10:56 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Modified Davidson's fixative use Dear colleagues, For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol? Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon Jun 8 12:25:34 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Jun 8 12:25:39 2009 Subject: [Histonet] Re: Modified Davidson's fixative use Message-ID: Teri Johnson, HT(ASCP)QIHC, Managing Director Histology Facility, Stowers Institute for Medical Research in Kansas City, Missouri asks: >>For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol?<< When I use modified Davidson's fixative as a disclosing fixative for lymph nodes in colon resection specimens, I'll wash the tissue briefly to reduce the smell, since my gross desk is unventilated (long story). The cassettes containing the dissected lymph nodes will go into neutral buffered formalin at least briefly before being processed through alcohols. Bob Richmond Samurai Pathologist Knoxville TN From janderson <@t> halozyme.com Mon Jun 8 12:28:02 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Mon Jun 8 12:28:50 2009 Subject: [Histonet] quenching auto-fluorescence Message-ID: Good morning. I'm wondering how to quench auto-fluorescence in tissue from a mouse that was treated with a FITC-labeled protein. What do people usually use to quench the endogenous auto-fluorescence, and will this treatment quench the FITC signal from the labeled protein? Thanks a lot for your help! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From gayle.callis <@t> bresnan.net Mon Jun 8 13:19:43 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Jun 8 13:20:07 2009 Subject: [Histonet] Re: Etching plastic for T blue staining Message-ID: <000101c9e865$b39f1ce0$1add56a0$@callis@bresnan.net> You wrote: Chris, If you do not do this, consider etching your tissue to remove the surface plastic. We use 0.2% formic acid - start with less than a minute as your tissues are thin. Some labs etch using ethyl alcohol or methanol. We have success. Using the formic acid is commonly done on undecalcified bone embedded in methyl methacrylate to achieve a mild surface decalcification of the bone. This removes a few micrometers of calcium from bone in thicker slab section (ground and polished) and on thinner microtomed MMA sections. However, I don't think the acid actually dissolves/removes plastic itself. This is why acid etched plastic bone permit certain low molecular weight dyes (toludine blue, basic fuchsin, methylene blue and dye mixtures e.g. Sanderson's rapid bone stain and Shenks recipe MacNeals tertrachrome) to penetrate into the bone matrix of thicker slabs. However, etching the plastic with alcohols is a good way to soften and possibly remove some of the plastic to allow better penetration of dyes into the soft tissues. Glycol methacrylate is not removable, MMA is removable using xylene and some other solvents, and if you use an EM resin, you may have to do sodium ethoxide treatment to remove plastic. For EM resin embedded sections at 1 um or so, we uses Toluidine blue/sodium borate , pH 11, by flooding a section then heating on a hot plate, rinsing, drying and coverslippling. Most of the time, tissues embedded in GMA or MMA will stain with a toluidine blue method, particularly when the pH is 8 or higher. There is a very good discussion on the etching, dyes, pH and temperature effects on various plastics in this publication. Horobin RW. Staining plastic sections: a review of problems, explanations, and possible solutions. J Microscopy 131:173-186, 1982. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 From gayle.callis <@t> bresnan.net Mon Jun 8 13:46:51 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Jun 8 13:47:17 2009 Subject: [Histonet] Quenching autofluorescence Message-ID: <000501c9e869$7df15d70$79d41850$@callis@bresnan.net> You wrote: I'm wondering how to quench auto-fluorescence in tissue from a mouse that was treated with a FITC-labeled protein. What do people usually use to quench the endogenous auto-fluorescence, and will this treatment quench the FITC signal from the labeled protein? Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 First, you didn't say how the tissues were treated after removal from animal? Are you doing FFPE or frozen sections, etc???? Was this an injected protein-FITC? More details will help. A method to remove auto-fluorescence will depend on the type of auto-fluorescence you experience due to the fact there are more than one source of this problem. An excellent discussion with methods for removal are found on the Wright Cell Imaging Facility website. http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 From GoodwinD <@t> pahosp.com Mon Jun 8 13:51:21 2009 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Mon Jun 8 13:52:11 2009 Subject: [Histonet] Preservation/transport medium for tissue culture Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C4A1@uphsmbx2.UPHS.PENNHEALTH.PRV> Greetings. My knowledge of tissue culture is non-existent. What is the recommended preservative/transport medium for tissue (namely foreskin) to be used for tissue culture that has a viability factor of 1-2 weeks? Also need the storage requirements. Thanks in advance! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From emerald_lake77 <@t> yahoo.com Mon Jun 8 14:48:14 2009 From: emerald_lake77 <@t> yahoo.com (emerald_lake77@yahoo.com) Date: Mon Jun 8 14:48:17 2009 Subject: [Histonet] Low pH HIER -- Tris-HCl buffer Message-ID: <382303.66672.qm@web110603.mail.gq1.yahoo.com> Hello all, ? Has anyone ever tried using a Tris buffer solution at very low pH (pH 1 or 2) to retrieve epitopes whether through a microwave, steamer or pressure cooker?? I am trying to retrieve 10%NBF, 24-48 hr fixed?mouse and rat tissue for an antigen that papers and books suggest should be retrieved using low pH Tris buffer. ? I was just wondering if anyone has had any experience(s) to share ... buffer solution recipes?and subsequent positive or negative (e.g. poor) results. ? Thank you. ? Gustave ? Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive ? Keywords: low ph HIER, retrieval, retreival, epitope, antibody, tris, tris-hcl, buffer From lenaspencer <@t> insightbb.com Mon Jun 8 16:50:58 2009 From: lenaspencer <@t> insightbb.com (Lena Spencer) Date: Mon Jun 8 16:51:03 2009 Subject: [Histonet] FW: new reference books Message-ID: <001801c9e883$354a9590$9fdfc0b0$@com> From: Lena Spencer [mailto:lenaspencer@insightbb.com] Sent: Sunday, June 07, 2009 7:45 PM To: 'histonet-request@lists.utsouthwestern.edu' Subject: new reference books Hi All: I know that many of you have been looking for new reference books. There are now two new ones on the market. Freida Carson's, 3rd edition is hot off the ASCP press and available for purchase from the ASCP go to the webpage and an order, www.ascp.org . '0Histologic Preparations Common Problems and Their Solutions" is an Atlas that covers most common special stains, processing and microtomy problem. This book has pictures of good and bad examples of stains, there is a discussion about each stain, controls, problems encounter and possible solutions, references and so much more. There will be a sample chapter (or part of a chapter) in CAP Today. The Atlas is written by NSH Members who served on the HistoQIP Committee and Edited by Dr. Richard Brown, who served as Chairman of the committee. The Atlas can be purchased on the CAP webpage - www.cap.org Hopefully you will find these two books great references for your laboratory and your personal library. Lena Spencer From AnthonyH <@t> chw.edu.au Mon Jun 8 18:28:54 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jun 8 18:29:09 2009 Subject: [Histonet] Storage of tissues in PFA beforedehydrationandembedding In-Reply-To: <200906081049524527524@foxmail.com> Message-ID: Then you will have to use frozen sections not FFPE tissues. May be one of the formalin-free fixatives might work. You will need to experiment and see =20 =20 Regards=20 Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)=20 Laboratory Manager & Senior Scientist=20 Tel: 612 9845 3306=20 Fax: 612 9845 3318=20 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA=20 -----Original Message----- From: TF [mailto:tifei@foxmail.com]=20 Sent: Monday, 8 June 2009 12:50 PM To: Patsy Ruegg; Tony Henwood; histonet@lists.utsouthwestern.edu Subject: Re: RE: [Histonet] Storage of tissues in PFA beforedehydrationand= embedding =09 =09 Just want to mention a point that some antigens are too fragile to PFA and= in my hand can not be retrieval"ed" anymore...such as rat CD31. =20 =20 =20 2009-06-08=20 =09 ________________________________ TF=20 =09 ________________________________ =B7=A2=BC=FE=C8=CB=A3=BA Patsy Ruegg=20 =B7=A2=CB=CD=CA=B1=BC=E4=A3=BA 2009-06-08 00:13:46=20 =CA=D5=BC=FE=C8=CB=A3=BA 'Tony Henwood'; histonet@lists.utsouthwestern.edu= =20 =B3=AD=CB=CD=A3=BA=20 =D6=F7=CC=E2=A3=BA RE: [Histonet] Storage of tissues in PFA beforedehydrat= ionandembedding=20 =09 =09 I agree with Tony, the problem is usually underfixation not over with aldehyde fixatives, if you do not stabilize the proteins by fixing (Bryan Hewlett says this takes 24 hrs. no matter the size of the tissue) paraffin processing will adversely affect your tissue and in some cases the proteins of interest will be washed away and lost with no change or no amount of AR to get them back. Researchers are stuck in the days before AR techniques when cross linking of proteins by aldehyde fixation was a problem because = we did not know how to retrieve them, but now we do, and the bigger problem is losing antigens from paraffin processing because they have not been adequately fixed to protect them. I recommend 24-72 hour fixation in aldehyde fixative and then the tissues can be placed in 70% alcohol. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net=20 www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwo= od Sent: Thursday, June 04, 2009 5:22 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydrationandembedding Nick, Well after 30 years of doing ICCs, the worst results are nearly always with tissues that have not been adequately fixed in formalin. This is based on using over 300 different antibodies in both adult and pediatric settings. The following paper shows an example of what can go wrong with under-fixed tissue: Gomes L, Mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge" J Histotechnol 31(4):138-184 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead=20 Cnr Hawkesbury Road and Hainsworth Street, Westmead=20 Locked Bag 4001, Westmead NSW 2145, AUSTRALIA=20 -----Original Message----- From: Nicholas David Evans [mailto:ndevans@stanford.edu]=20 Sent: Friday, 5 June 2009 8:56 AM To: Tony Henwood Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Thanks for the helpful response.=20 After a consensus of views I decided to store at 70% ethanol for several days following 3-4hrs in 4% PFA. I think many people use paraffin embedded tissues for ICC and over-fixation is deleterious for this. 4% Paraformaldehyde (PFA) =3D 4g PFA dissolved in 100 mL PBS. Indeed when in solution it is present as formaldehyde. I will consider your suggestion of frozen sections if I have poor results. Thanks again for the e mail and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au]=20 Sent: 03 June 2009 16:54 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA=20 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, =20 I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol =3D 6 hours, before overnight in 100%) and embedding of all samples on the same day.=20 =20 I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way.=20 =20 With thanks and best wishes Nick Evans =20 Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended = solely for the use of the individual or entity to whom they are addressed. = If you are not the intended recipient, please delete it and notify the send= er. Views expressed in this message and any attachments are those of the indivi= dual sender, and are not necessarily the views of The Children's Hospital a= t Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens= Hospital at Westmead accepts no liability for any consequential damage res= ulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Mon Jun 8 18:34:23 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jun 8 18:34:29 2009 Subject: [Histonet] Preservation/transport medium for tissue culture In-Reply-To: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C4A1@uphsmbx2.UPHS.PENNHEALTH.PRV> Message-ID: Diana, I would use Hank's solution. Sigma Cat No.H9269-100ml Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, Diana Sent: Tuesday, 9 June 2009 4:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preservation/transport medium for tissue culture Greetings. My knowledge of tissue culture is non-existent. What is the recommended preservative/transport medium for tissue (namely foreskin) to be used for tissue culture that has a viability factor of 1-2 weeks? Also need the storage requirements. Thanks in advance! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From carl.hobbs <@t> kcl.ac.uk Tue Jun 9 05:36:16 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Tue Jun 9 05:37:54 2009 Subject: [Histonet] Re: MAP2 and GFAP double staining Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0EA3@KCL-MAIL04.kclad.ds.kcl.ac.uk> Antibodies MAP2 Sigma M4403 GFAP Dako Z0334 You don't tell what application nor species, nor any detection details. I have not done double-labelling with these two but they individually work very, very well in frozen/pwax sections of human, mouse and rat so they will be fine for double-labelling. carl From melissa.ribeiro <@t> brinegroup.com Tue Jun 9 09:13:45 2009 From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro) Date: Tue Jun 9 09:13:52 2009 Subject: [Histonet] Histology Manager opportunity in Connecticut Message-ID: <43904A2EECEAB54D8A023931049FEA4C12AA9B@brin-sbs01.brinegroup.local> Brine Group conducting a search for a HISTOLOGY MANAGER in a community hospital based clinical lab in Southeastern Connecticut. Looking for strong leadership skills, a vision of future technologies for Histology, and ability to interact with pathologists to keep the section on the "cutting edge". MAJOR ACCOUNTABILITIES/CRITICAL RESPONSIBILITIES Responsible for daily operations within the Histology department: * Manages and supervises Histology staff ensuring the department runs effectively. * Responsible for maintaining and revising Histology policies and procedures. * Responsible for developing and monitoring section budget. Reviews budget monthly for variances and eliminating negative variances. * Hires qualified staff, disciplines and terminate staff as appropriate in a consistent and timely fashion. * Conducts staff meetings, keeping staff informed about departmental and hospital wide information. * Ensure staff competency by assessing educational and developmental needs of the staff, developing and maintaining competency program and completes annual performance reviews in a timely fashion. * Effectively troubleshoots and resolve technical and clinical issues. * Develops and monitors Quality Indicators. Identifies trends and acts on opportunities for improvement. * Ensure adequate staffing for department and coverage for all shifts. * Ensures compliance with all Laboratory and Hospital safety policies as well as other Hospital policies. * Responsible for department QC procedures and troubleshoots QC failures with proper documentation. * Prepares and monitors daily/weekly/monthly quality control for Histology. * Participates in inter and intra-department QA initiatives. * Responds to technical issues from physicians and works to appropriately resolve them. * Maintains technical competency and keeps current on new testing available for Histology. * Acts as a resource person for new Histology testing/instrumentation. * Effectively utilizes all necessary LIS functions. * Maintains appropriate levels of inventory. * Responsible for Histology in all Laboratory and Hospital inspections; CAP, JCAHO and the State of Connecticut. QUALIFICATIONS/REQUIREMENTS Education: Minimum B.S. degree in Laboratory Science. Prefer graduate of formal Histology training program. Experience: Working knowledge of gross human anatomy required. Five years of Histology experience with a superior performance record required. Previous supervisory experience preferred. Knowledgeable of current and new technologies in Histology. Training: Histologic Technique required. Licensure: ASCP certification preferred. All interested/qualified candidates please contact Melissa Ribeiro at (781) 272-3400 ext. 228 or via e-mail at mribeiro@brinegroup.com Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 From laurie.colbert <@t> huntingtonhospital.com Tue Jun 9 10:38:24 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Jun 9 10:38:30 2009 Subject: [Histonet] Gram Stain Message-ID: <57BE698966D5C54EAE8612E8941D768305CE8209@EXCHANGE3.huntingtonhospital.com> Can anyone recommend a good gram stain kit? We currently do a modified Brown-Hopps stain and make up all of the reagents, but I am looking for a kit. Laurie Colbert From gu.lang <@t> gmx.at Tue Jun 9 11:49:20 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jun 9 11:49:30 2009 Subject: [Histonet] antibody recommendation Message-ID: <128F56C6DCDE4207937936DBE9A007FC@dielangs.at> Hi! Can anyone help me with a recommendation for CDK4 and MDM2 antibody for human FFPE tissue? We use the Benchmark XT as immunhisto-instrument. Thanks in advance Gudrun Lang From syinan <@t> ucalgary.ca Tue Jun 9 12:18:40 2009 From: syinan <@t> ucalgary.ca (Salim Yalcin Inan) Date: Tue Jun 9 12:19:54 2009 Subject: [Histonet] Rat Perfusion Message-ID: Hello, Is there anyone using peristaltic pump during rat intracardiac perfusion for immuno? I will order one and would like to ask for your suggestions and comments. Any brand and model name/number? Please e-mail me at: syinan@ucalgary.ca Thank you very much in advance. Salim From jo-ann.bader <@t> mcgill.ca Tue Jun 9 12:17:45 2009 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Tue Jun 9 12:21:17 2009 Subject: [Histonet] SOP"S Message-ID: <76D119EF12C904418800ED67CCB2062905DEF797EC@EXMBXVS1.campus.mcgill.ca> Good Day All, We have currently merged two histology groups to form a Histology Core. We are currently going over all our SOP's and we were wondering if some of you would share your SOP'S so we could have a better idea if we are in the same ball park as others. We are looking mainly at processin, embedding and staining SOP'S. Thanks in advance Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 From jennifer.harvey <@t> vanderbilt.edu Tue Jun 9 12:23:24 2009 From: jennifer.harvey <@t> vanderbilt.edu (Jennifer Harvey) Date: Tue Jun 9 12:23:28 2009 Subject: [Histonet] STUF Message-ID: Can anyone tell me what is in STUF antigen retrieval reagent? Thanks Jennifer Harvey, HT(ASCP) QIHC Core Histologist Vanderbilt Vision Research Center 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 Fax: 615-936-1378 email: jennifer.harvey@vanderbilt.edu From settembr <@t> umdnj.edu Tue Jun 9 12:31:51 2009 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jun 9 12:32:07 2009 Subject: [Histonet] antibody recommendation MDM2 Message-ID: Hello Gudrun, I use MDM2 by BD cat# 556353 I use Ant. Ret. and use it at a 1:400 dilution. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 06/09/09 12:49 PM >>> Hi! Can anyone help me with a recommendation for CDK4 and MDM2 antibody for human FFPE tissue? We use the Benchmark XT as immunhisto-instrument. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Tue Jun 9 14:31:03 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Jun 9 14:31:18 2009 Subject: [Histonet] temp histo position at Medimmune in Gaithersburg In-Reply-To: References: Message-ID: We have an immediate opening for a temporary histotech at Medimmune HQ in Gaithersburg, MD outside of DC. No relocation. Mainly routine paraffin histology on rodent tissues, lots of it! Processing, thru HE and Specials, IHC, frozen and necropsy experience a plus. Email me if you want further details. madaryj@medimmune.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, June 09, 2009 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 67, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Modified Davidson's fixative use (Jackie M O'Connor) 2. Re: Modified Davidson's fixative use (Robert Richmond) 3. quenching auto-fluorescence (Jennifer Anderson) 4. Re: Etching plastic for T blue staining (gayle callis) 5. Quenching autofluorescence (gayle callis) 6. Preservation/transport medium for tissue culture (Goodwin, Diana) 7. Low pH HIER -- Tris-HCl buffer (emerald_lake77@yahoo.com) 8. FW: new reference books (Lena Spencer) 9. RE: RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding (Tony Henwood) 10. RE: Preservation/transport medium for tissue culture (Tony Henwood) 11. Re: MAP2 and GFAP double staining (Hobbs, Carl) 12. Histology Manager opportunity in Connecticut (Melissa Ribeiro) 13. Gram Stain (Laurie Colbert) 14. antibody recommendation (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Mon, 8 Jun 2009 12:06:13 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] Modified Davidson's fixative use To: "Johnson, Teri" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" To not answer your question - we fix eyes and testes in Modified Davidsons for 48 hours then transfer to formalin to await processing. Since mDF is basically formalin, alcohol, and gaa - a water rinse step is pretty moot. "Johnson, Teri" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2009 10:56 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Modified Davidson's fixative use Dear colleagues, For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol? Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 8 Jun 2009 13:25:34 -0400 From: Robert Richmond Subject: [Histonet] Re: Modified Davidson's fixative use To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Teri Johnson, HT(ASCP)QIHC, Managing Director Histology Facility, Stowers Institute for Medical Research in Kansas City, Missouri asks: >>For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol?<< When I use modified Davidson's fixative as a disclosing fixative for lymph nodes in colon resection specimens, I'll wash the tissue briefly to reduce the smell, since my gross desk is unventilated (long story). The cassettes containing the dissected lymph nodes will go into neutral buffered formalin at least briefly before being processed through alcohols. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Mon, 8 Jun 2009 10:28:02 -0700 From: "Jennifer Anderson" Subject: [Histonet] quenching auto-fluorescence To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Good morning. I'm wondering how to quench auto-fluorescence in tissue from a mouse that was treated with a FITC-labeled protein. What do people usually use to quench the endogenous auto-fluorescence, and will this treatment quench the FITC signal from the labeled protein? Thanks a lot for your help! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 janderson@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ------------------------------ Message: 4 Date: Mon, 8 Jun 2009 12:19:43 -0600 From: "gayle callis" Subject: [Histonet] Re: Etching plastic for T blue staining To: "'Histonet'" Message-ID: <000101c9e865$b39f1ce0$1add56a0$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" You wrote: Chris, If you do not do this, consider etching your tissue to remove the surface plastic. We use 0.2% formic acid - start with less than a minute as your tissues are thin. Some labs etch using ethyl alcohol or methanol. We have success. Using the formic acid is commonly done on undecalcified bone embedded in methyl methacrylate to achieve a mild surface decalcification of the bone. This removes a few micrometers of calcium from bone in thicker slab section (ground and polished) and on thinner microtomed MMA sections. However, I don't think the acid actually dissolves/removes plastic itself. This is why acid etched plastic bone permit certain low molecular weight dyes (toludine blue, basic fuchsin, methylene blue and dye mixtures e.g. Sanderson's rapid bone stain and Shenks recipe MacNeals tertrachrome) to penetrate into the bone matrix of thicker slabs. However, etching the plastic with alcohols is a good way to soften and possibly remove some of the plastic to allow better penetration of dyes into the soft tissues. Glycol methacrylate is not removable, MMA is removable using xylene and some other solvents, and if you use an EM resin, you may have to do sodium ethoxide treatment to remove plastic. For EM resin embedded sections at 1 um or so, we uses Toluidine blue/sodium borate , pH 11, by flooding a section then heating on a hot plate, rinsing, drying and coverslippling. Most of the time, tissues embedded in GMA or MMA will stain with a toluidine blue method, particularly when the pH is 8 or higher. There is a very good discussion on the etching, dyes, pH and temperature effects on various plastics in this publication. Horobin RW. Staining plastic sections: a review of problems, explanations, and possible solutions. J Microscopy 131:173-186, 1982. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 ------------------------------ Message: 5 Date: Mon, 8 Jun 2009 12:46:51 -0600 From: "gayle callis" Subject: [Histonet] Quenching autofluorescence To: "'Histonet'" Message-ID: <000501c9e869$7df15d70$79d41850$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" You wrote: I'm wondering how to quench auto-fluorescence in tissue from a mouse that was treated with a FITC-labeled protein. What do people usually use to quench the endogenous auto-fluorescence, and will this treatment quench the FITC signal from the labeled protein? Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 First, you didn't say how the tissues were treated after removal from animal? Are you doing FFPE or frozen sections, etc???? Was this an injected protein-FITC? More details will help. A method to remove auto-fluorescence will depend on the type of auto-fluorescence you experience due to the fact there are more than one source of this problem. An excellent discussion with methods for removal are found on the Wright Cell Imaging Facility website. http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 ------------------------------ Message: 6 Date: Mon, 8 Jun 2009 14:51:21 -0400 From: "Goodwin, Diana" Subject: [Histonet] Preservation/transport medium for tissue culture To: Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C4A1@uphsmbx2.UPHS.PENNHEALTH.PRV> Content-Type: text/plain; charset="US-ASCII" Greetings. My knowledge of tissue culture is non-existent. What is the recommended preservative/transport medium for tissue (namely foreskin) to be used for tissue culture that has a viability factor of 1-2 weeks? Also need the storage requirements. Thanks in advance! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 7 Date: Mon, 8 Jun 2009 12:48:14 -0700 (PDT) From: emerald_lake77@yahoo.com Subject: [Histonet] Low pH HIER -- Tris-HCl buffer To: Histonet@lists.utsouthwestern.edu Message-ID: <382303.66672.qm@web110603.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello all, ? Has anyone ever tried using a Tris buffer solution at very low pH (pH 1 or 2) to retrieve epitopes whether through a microwave, steamer or pressure cooker?? I am trying to retrieve 10%NBF, 24-48 hr fixed?mouse and rat tissue for an antigen that papers and books suggest should be retrieved using low pH Tris buffer. ? I was just wondering if anyone has had any experience(s) to share ... buffer solution recipes?and subsequent positive or negative (e.g. poor) results. ? Thank you. ? Gustave ? Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive ? Keywords: low ph HIER, retrieval, retreival, epitope, antibody, tris, tris-hcl, buffer ------------------------------ Message: 8 Date: Mon, 8 Jun 2009 17:50:58 -0400 From: "Lena Spencer" Subject: [Histonet] FW: new reference books To: Message-ID: <001801c9e883$354a9590$9fdfc0b0$@com> Content-Type: text/plain; charset="us-ascii" From: Lena Spencer [mailto:lenaspencer@insightbb.com] Sent: Sunday, June 07, 2009 7:45 PM To: 'histonet-request@lists.utsouthwestern.edu' Subject: new reference books Hi All: I know that many of you have been looking for new reference books. There are now two new ones on the market. Freida Carson's, 3rd edition is hot off the ASCP press and available for purchase from the ASCP go to the webpage and an order, www.ascp.org . '0Histologic Preparations Common Problems and Their Solutions" is an Atlas that covers most common special stains, processing and microtomy problem. This book has pictures of good and bad examples of stains, there is a discussion about each stain, controls, problems encounter and possible solutions, references and so much more. There will be a sample chapter (or part of a chapter) in CAP Today. The Atlas is written by NSH Members who served on the HistoQIP Committee and Edited by Dr. Richard Brown, who served as Chairman of the committee. The Atlas can be purchased on the CAP webpage - www.cap.org Hopefully you will find these two books great references for your laboratory and your personal library. Lena Spencer ------------------------------ Message: 9 Date: Tue, 9 Jun 2009 09:28:54 +1000 From: "Tony Henwood" Subject: RE: RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding To: , "Patsy Ruegg" , Message-ID: Content-Type: text/plain; charset="gb2312" Then you will have to use frozen sections not FFPE tissues. May be one of the formalin-free fixatives might work. You will need to experiment and see Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: TF [mailto:tifei@foxmail.com] Sent: Monday, 8 June 2009 12:50 PM To: Patsy Ruegg; Tony Henwood; histonet@lists.utsouthwestern.edu Subject: Re: RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding Just want to mention a point that some antigens are too fragile to PFA and in my hand can not be retrieval"ed" anymore...such as rat CD31. 2009-06-08 ________________________________ TF ________________________________ ???????? Patsy Ruegg ?????????? 2009-06-08 00:13:46 ???????? 'Tony Henwood'; histonet@lists.utsouthwestern.edu ?????? ?????? RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding I agree with Tony, the problem is usually underfixation not over with aldehyde fixatives, if you do not stabilize the proteins by fixing (Bryan Hewlett says this takes 24 hrs. no matter the size of the tissue) paraffin processing will adversely affect your tissue and in some cases the proteins of interest will be washed away and lost with no change or no amount of AR to get them back. Researchers are stuck in the days before AR techniques when cross linking of proteins by aldehyde fixation was a problem because we did not know how to retrieve them, but now we do, and the bigger problem is losing antigens from paraffin processing because they have not been adequately fixed to protect them. I recommend 24-72 hour fixation in aldehyde fixative and then the tissues can be placed in 70% alcohol. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, June 04, 2009 5:22 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydrationandembedding Nick, Well after 30 years of doing ICCs, the worst results are nearly always with tissues that have not been adequately fixed in formalin. This is based on using over 300 different antibodies in both adult and pediatric settings. The following paper shows an example of what can go wrong with under-fixed tissue: Gomes L, Mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge" J Histotechnol 31(4):138-184 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nicholas David Evans [mailto:ndevans@stanford.edu] Sent: Friday, 5 June 2009 8:56 AM To: Tony Henwood Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Thanks for the helpful response. After a consensus of views I decided to store at 70% ethanol for several days following 3-4hrs in 4% PFA. I think many people use paraffin embedded tissues for ICC and over-fixation is deleterious for this. 4% Paraformaldehyde (PFA) = 4g PFA dissolved in 100 mL PBS. Indeed when in solution it is present as formaldehyde. I will consider your suggestion of frozen sections if I have poor results. Thanks again for the e mail and best wishes Nick -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 03 June 2009 16:54 To: Nicholas David Evans; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of tissues in PFA before dehydration andembedding Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is immunohistochemistry using formalin-labile antigens that you are worried about then use frozen sections not FFPE sections. The major cause of poor morphology in FFPE tissues is inadequate formalin fixation. If you want ethanol fixed tissues (and rotten morphology) then leave out the formalin fixation step altogether. AND how do you fix it in 4% polyformaldehyde? Surely when it is in solution it becomes formaldehyde? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Thursday, 4 June 2009 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of tissues in PFA before dehydration andembedding Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As I value my weekends, I would obviously prefer to do the dehydration (which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95, 100% ethanol = 6 hours, before overnight in 100%) and embedding of all samples on the same day. I understand that storage in PFA for longer than 24 hrs may adversely affect my tissues. Is it possible to store for longer in a lower concentration of PFA, or in another buffer until I am ready to dehydrate and embed? In short, I would appreciate if anyone could suggest a way to plan this experiment in the most efficient way. With thanks and best wishes Nick Evans Dept Surgery Stanford University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 10 Date: Tue, 9 Jun 2009 09:34:23 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Preservation/transport medium for tissue culture To: "Goodwin, Diana" , Message-ID: Content-Type: text/plain; charset="us-ascii" Diana, I would use Hank's solution. Sigma Cat No.H9269-100ml Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, Diana Sent: Tuesday, 9 June 2009 4:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preservation/transport medium for tissue culture Greetings. My knowledge of tissue culture is non-existent. What is the recommended preservative/transport medium for tissue (namely foreskin) to be used for tissue culture that has a viability factor of 1-2 weeks? Also need the storage requirements. Thanks in advance! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@ pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 11 Date: Tue, 9 Jun 2009 11:36:16 +0100 From: "Hobbs, Carl" Subject: [Histonet] Re: MAP2 and GFAP double staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0EA3@KCL-MAIL04.kclad.ds.kcl.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Antibodies MAP2 Sigma M4403 GFAP Dako Z0334 You don't tell what application nor species, nor any detection details. I have not done double-labelling with these two but they individually work very, very well in frozen/pwax sections of human, mouse and rat so they will be fine for double-labelling. carl ------------------------------ Message: 12 Date: Tue, 9 Jun 2009 10:13:45 -0400 From: "Melissa Ribeiro" Subject: [Histonet] Histology Manager opportunity in Connecticut To: , Message-ID: <43904A2EECEAB54D8A023931049FEA4C12AA9B@brin-sbs01.brinegroup.local> Content-Type: text/plain; charset="us-ascii" Brine Group conducting a search for a HISTOLOGY MANAGER in a community hospital based clinical lab in Southeastern Connecticut. Looking for strong leadership skills, a vision of future technologies for Histology, and ability to interact with pathologists to keep the section on the "cutting edge". MAJOR ACCOUNTABILITIES/CRITICAL RESPONSIBILITIES Responsible for daily operations within the Histology department: * Manages and supervises Histology staff ensuring the department runs effectively. * Responsible for maintaining and revising Histology policies and procedures. * Responsible for developing and monitoring section budget. Reviews budget monthly for variances and eliminating negative variances. * Hires qualified staff, disciplines and terminate staff as appropriate in a consistent and timely fashion. * Conducts staff meetings, keeping staff informed about departmental and hospital wide information. * Ensure staff competency by assessing educational and developmental needs of the staff, developing and maintaining competency program and completes annual performance reviews in a timely fashion. * Effectively troubleshoots and resolve technical and clinical issues. * Develops and monitors Quality Indicators. Identifies trends and acts on opportunities for improvement. * Ensure adequate staffing for department and coverage for all shifts. * Ensures compliance with all Laboratory and Hospital safety policies as well as other Hospital policies. * Responsible for department QC procedures and troubleshoots QC failures with proper documentation. * Prepares and monitors daily/weekly/monthly quality control for Histology. * Participates in inter and intra-department QA initiatives. * Responds to technical issues from physicians and works to appropriately resolve them. * Maintains technical competency and keeps current on new testing available for Histology. * Acts as a resource person for new Histology testing/instrumentation. * Effectively utilizes all necessary LIS functions. * Maintains appropriate levels of inventory. * Responsible for Histology in all Laboratory and Hospital inspections; CAP, JCAHO and the State of Connecticut. QUALIFICATIONS/REQUIREMENTS Education: Minimum B.S. degree in Laboratory Science. Prefer graduate of formal Histology training program. Experience: Working knowledge of gross human anatomy required. Five years of Histology experience with a superior performance record required. Previous supervisory experience preferred. Knowledgeable of current and new technologies in Histology. Training: Histologic Technique required. Licensure: ASCP certification preferred. All interested/qualified candidates please contact Melissa Ribeiro at (781) 272-3400 ext. 228 or via e-mail at mribeiro@brinegroup.com Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 ------------------------------ Message: 13 Date: Tue, 9 Jun 2009 08:38:24 -0700 From: "Laurie Colbert" Subject: [Histonet] Gram Stain To: "Histonet" Message-ID: <57BE698966D5C54EAE8612E8941D768305CE8209@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Can anyone recommend a good gram stain kit? We currently do a modified Brown-Hopps stain and make up all of the reagents, but I am looking for a kit. Laurie Colbert ------------------------------ Message: 14 Date: Tue, 9 Jun 2009 18:49:20 +0200 From: "Gudrun Lang" Subject: [Histonet] antibody recommendation To: Message-ID: <128F56C6DCDE4207937936DBE9A007FC@dielangs.at> Content-Type: text/plain; charset="us-ascii" Hi! Can anyone help me with a recommendation for CDK4 and MDM2 antibody for human FFPE tissue? We use the Benchmark XT as immunhisto-instrument. Thanks in advance Gudrun Lang ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 9 *************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Montina.VanMeter <@t> pbrc.edu Tue Jun 9 15:29:52 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Tue Jun 9 15:32:43 2009 Subject: [Histonet] 2009 Louisiana Society for Histotechnology Annual Symposium/Convention Message-ID: <4FE7FB862E90E448AE32388E759220E501577AA9@pbrcas31.pbrc.edu> Fellow Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 26th Annual Symposium/Convention: "Your Histeaux Surplus Package" June 12 & 13, 2009 at the Bourbon Orleans Hotel 717 Orleans St. New Orleans, LA 70117 www.bourbonorleans.com 1. Walk-ins are always welcome! If you are planning on registering the day of the meeting we would appreciate it if you would send us an email or call Dixie 337-233-1951, dixiehistochick@bellsouth.net or Tina 225-603-0953, vanmetmj@pbrc.edu prior to the meeting, which will allow the LSH to make arrangements for workshop handouts and food reservations. Please make additional copies of your registration form for co-workers in your lab or facility that might be interested in attending. The LSH would also like to extend the invitation to our fellow technologists and pathologists from surrounding states. We encourage everyone to attend in order to build our networking potential, earn those valuable CEU's and enjoy the beautiful city of New Orleans. 2. The LSH room rates will be honored as long as rooms are available. For those attendees who may want to visit the beautiful sights and sounds of New Orleans, the Bourbon Orleans Hotel will honor the room rates for three days prior and three days after the meeting. Special on-site parking rates for LSH attendees is available as well as public parking around Jackson Square. For reservations call 1-504-523-2222 and mention you are with the LSH group. 3. There are many local attractions going on during the week of our symposium such as: The Cajun - Zydeco Festival, Seafood Festival and the Creole Tomato Festival all in the French Quarter/Jackson Square area. Free admission to the festivals. Check out the links below: http://www.neworleansonline.com/news/2009/May/vieuxtodo.html http://ww.neworleansonline.com/neworleans/tours/ 4. Employment Postings: The LSH will have a "Job Board" for anyone who would like to advertise opportunities for employment at their facility. Please contact us via email, phone or at the meeting. 5. 2009 LSH State Meeting Workshops: WS#1 - Am I Really Ready for this CAP Inspection? WS#2 - Mouse to Horse: It's Not Human WS#3 - Breast Cancer and the Standardization of HER2/neu Testing WS#4 - Contemporary Trends in Immunohistochemistry WS#5 - Troubleshooting Routine Special Stains WS#6 - Which Code Do I Use? (CPT Coding) WS#7 - Are You REALLY Ready for the Next Catastrophic Event That Will Affect Your Hospital or City? WS#8 - 2 Crazy Cat Ladies Give Their Opinions of Life, Liberty, and Lab Management We have a variety of topics presented by experienced speakers that promises to benefit everyone. The attendees will have access to several scientific vendor exhibits during the entire symposium. The LSH would love to have y'all come on down to the Bourbon Orleans Hotel in the French Quarter! Hope to see you in June! The LSH Committee Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 From tjasper <@t> copc.net Tue Jun 9 20:04:06 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Jun 9 20:04:12 2009 Subject: [Histonet] Histologist Position in Bend, Oregon Message-ID: <90354A475B420441B2A0396E5008D4965E304C@copc-sbs.COPC.local> Fellow Histonetters, A position has opened for a Histologist here in beautiful Bend, Oregon... Central Oregon Regional Pathology Services - Bend, Oregon seeks a full-time Histologist, HT, HTL or registry eligible preferred. Responsibilities include - Embedding, Microtomy, Routine Staining, Automated Special Staining and Immunohistochemistry. Excellent benefits, including health insurance, retirement plan and competitive salary. Potential sign-on bonus as well. Bend is located in central Oregon at the base of the Cascade Mountains. World class outdoor activities abound, including biking, hiking, rock climbing, fishing and camping. Phenomenal alpine and nordic skiing available October through May. Bend is uniquely situated with the Cascades to the west and the high desert to the east. Although in the Pacific Northwest, Bend enjoys warm, clear days and cool evenings most of the year. Winters are mild with a clean environment and accessible wilderness. In about 30 minutes you can reach the scenic beauty of canyons and rock formations in the high desert. Crater Lake national park is approximately 100 miles south and the Pacific coast can be reached in just over 3 hours. The lush Willamette Valley lies between the Cascades and the coast and is stunningly beautiful as well. Many wonderful restaurants of varied cuisine are located all over town, outstanding wine is produced locally and 4 microbreweries produce top notch beer. Bend has a community symphony orchestra, a diverse musical scene and many art and cultural events. The Univ. of Oregon and Oregon State Univ. are in close proximity, and Portland is only a few hours away. We are a progressive, friendly lab and a great place to live. Contact us at - Central Oregon Regional Pathology Services 1348 NE Cushing Dr. Bend, OR 97701 Attn. Pam Sylvester or fax resume to (541)693-2648 or, you may contact me as well, Tom Jasper at (541)693-2677 Thank you, Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 From DDittus787 <@t> aol.com Tue Jun 9 20:47:52 2009 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Tue Jun 9 20:48:46 2009 Subject: [Histonet] Fwd: Who do you know for this great opportunity? - Long Island Histology Supe... Message-ID: I am forwarding this for anyone looking for an opportunity. Please respond directly to the recruiter at the bottom of the job description. dana **************A Good Credit Score is 700 or Above. See yours in just 2 easy steps! (http://pr.atwola.com/promoclk/100126575x1221322977x1201367197/aol?redir=http://www.freecreditreport.com/pm/default.aspx?sc=668072&hmpgID=62&bcd= JunestepsfooterNO62) From DDittus787 <@t> aol.com Tue Jun 9 20:52:34 2009 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Tue Jun 9 20:53:47 2009 Subject: [Histonet] job opportunity Message-ID: I am forwarding this , correctly this time for anyone looking for a position. Please respond directly to the recruiter at the bottom of the job description. Thanks Dana I wanted to ask you a quick favor. I have a unique search assignment and considering your connections I thought you would be the person to ask. I was hoping that you could pay it forward or in this case pass it forward, this email that is, to someone who will get this message closer to the person I am looking for. Please scan this and send it on to whomever you think can get this message closer to the one I?m looking for. I appreciate any assistance and hope to be able to return a favor to you someday. We are looking for an experienced Histology Lab manager for the Long Island area, relo package available for the right person. This Lab doesn't believe in hierarchies, it promotes personal development, offers flexible schedules, has state of the art equipment and is located in a beautiful setting. This person will be accountable for the direct supervision and operation of the lab and staff. We need someone who can support the company?s growth oriented business plan and has an entrepreneurial spirit. This person will be vital to the overall success of the lab so the following characteristics are necessary: Great leadership skills. Ability to train others and compose training manuals Able to smoothly manage the workflow of the lab. Great attitude, energetic and pleasant personality. Knowledge of CAP, State and federal guidelines and regulations pertinent to laboratory safety and security and HIPAA regulations. The ideal candidate has around 4 or more years of laboratory training with 2 years supervisory experience. H.T. or H.T.L. certification and must have qualification to obtain NYS Clinical Laboratory Technologist certificate. No travel required. Must have a great personality, be a team player and have excellent customer service skills. Bi-lingual a big plus! Salary range is $80k to $100k depending on experience. full benefits, healthcare, health savings accounts, 401k and accrued vacation. If you know someone qualified for this position, please forward this information. For anyone interested, please forward your resume to _jennifer@phcconsulting.com_ (mailto:jennifer@phcconsulting.com) for consideration. Thanks again for your time and assistance. If I can ever be of assistance to you just let me know. Best Wishes, Jennifer **************A Good Credit Score is 700 or Above. See yours in just 2 easy steps! (http://pr.atwola.com/promoclk/100126575x1221322977x1201367197/aol?redir=http://www.freecreditreport.com/pm/default.aspx?sc=668072&hmpgID=62&bcd= JunestepsfooterNO62) From Diane_Maia <@t> bshsi.org Tue Jun 2 11:30:07 2009 From: Diane_Maia <@t> bshsi.org (Maia, Diane) Date: Wed Jun 10 10:24:10 2009 Subject: [Histonet] Rapid tissue processors Message-ID: <286502DFCE063644B07E517E8224B15E0B5E75@EDC-MAIL-02.ads.bshsi.com> I saw your message on Histonet. We are also looking at rapid processors, and are wondering if you have chosen one yet, and if so, which one? Any useful comments you can provide would be appreciated. Thank You, Diane Maia, MD Medical Director of Hospital Laboratories Bon Secours DePaul Medical Center Norfolk, VA ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From heinrich.lob <@t> gmail.com Wed Jun 3 12:27:48 2009 From: heinrich.lob <@t> gmail.com (Heinrich Lob) Date: Wed Jun 10 10:24:12 2009 Subject: [Histonet] CD3 antibody Message-ID: Does anyone know a good CD3 antibody for paraffin AND for frozen sections? Thanks Heinrich E. Lob, PhD Department of Medicine Division of Cardiology 101 Woodruff Circle, Room 3335 Atlanta, GA, 30322 From charles.scouten <@t> leica-microsystems.com Thu Jun 4 10:07:18 2009 From: charles.scouten <@t> leica-microsystems.com (Charles Scouten) Date: Wed Jun 10 10:24:14 2009 Subject: [Histonet] microtome calibration References: <4A2556C2020000820003AA85@GWIA1.umsmed.edu> Message-ID: It is not a "calibration" issue. Most likely cause it too shallow a blade angle, another is something is loose and wobbly. Try grasping the blade holder and see if it can be moved in any direction. The tissue pedestal. If something is loose, that is the cause. Cordially, Charles W. Scouten, Ph.D Innovation Manager Biosystems Division Leica Biosystems St. Louis LLC 5918 Evergreen Blvd. St. Louis, MO 63134 United States of America telephone +1 314 522 0300?? Ext. 342 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes Sent: Tuesday, June 02, 2009 4:44 PM To: Histonet Subject: [Histonet] microtome calibration Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gyula.Szabo <@t> ucsfmedctr.org Thu Jun 4 10:55:44 2009 From: Gyula.Szabo <@t> ucsfmedctr.org (Szabo, Gyula) Date: Wed Jun 10 10:24:15 2009 Subject: [Histonet] Immunhistochemical practise Message-ID: <8CDAE933173B6944820221FE4E7E54A9EB18492D@EXMBMCB11.ucsfmedicalcenter.org> Hi, My name is Gyula Szabo from UCSF. I have a research position and I must use some immunhistochemical process. I do it manual and I would like to make by myself almost every chemical solution. I have some method but I need some correct exam because I could not find any information about it. I need the correct method of making antibody diulent and protein blocking solution. I know that the conponents are PBS buffer and 1% BSA but I do not how much is it need. If I make for 1000 mL antibody diulent, how much is BSA or good idea if for 1000 mL PBS add 1 g BSA? I have the fallowing method for protein blocking: 5 g non fat dry milk (like BSA) + 100 mL PBS buffer, then ulta centrifuga using at 1 hour than filtering. If this method is correct how long time the blocking process and must I use for this step the humidity chamber? I am looking forward to hearing from you. Thank you. Gyula From rjbuesa <@t> yahoo.com Wed Jun 10 11:41:09 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 10 11:41:13 2009 Subject: [Histonet] Antibody diluent Message-ID: <732988.2881.qm@web65716.mail.ac4.yahoo.com> Hi Gyula: To prepare the antibody diluent you will need also a preservative (use sodium azide). The formula is: Bovine serum albumen ---- 1 mL PBS with 0.05% Tween 20 ---99 mL sodium azide --- 100 mg Ren? J. --- On Thu, 6/4/09, Szabo, Gyula wrote: From: Szabo, Gyula Subject: [Histonet] Immunhistochemical practise To: "'histonet@pathology.swmed.edu'" Date: Thursday, June 4, 2009, 11:55 AM Hi, My name is Gyula Szabo from UCSF. I have a research position and I must use some immunhistochemical process. I do it manual and I would like to make by myself almost every chemical solution. I have some method but I need some correct exam because I could not find any information about it. I need the correct method of making antibody diulent and protein blocking solution. I know that the conponents are PBS buffer and 1% BSA but I do not how much is it need. If I make for 1000 mL antibody diulent, how much is BSA or good idea if for 1000 mL PBS add 1 g BSA? I have the fallowing method for protein blocking: 5 g non fat dry milk (like BSA) + 100 mL PBS buffer, then ulta centrifuga using at 1 hour than filtering. If this method is correct how long time the blocking process and must I use for this step the humidity chamber? I am looking forward to hearing from you. Thank you. Gyula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From af46 <@t> buffalo.edu Wed Jun 10 11:55:44 2009 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Wed Jun 10 11:55:49 2009 Subject: [Histonet] F4-80 anti mouse Message-ID: Is anyone out there using F4-80 anti mouse (Biolegend) for macrophage. What is your protocol for Paraffin embedded sections? Any help will be appreciated. Currently we have no staining at 1 to 100 for the primary and antigen citrate buffer. thanks Annette Featherstone From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 10 12:18:37 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 10 12:18:43 2009 Subject: [Histonet] Gram kit Message-ID: <65365F35C0F2EF4D846EC3CA73E49C437A9BFA9867@HPEMX3.HealthPartners.int> We switched to the "Huckert-Twort" Gram stain kit from Newcomer and our pathologists love it, especially our dermatopathologists! We were doing the Brown-Brenn. Their number is 1-800-383-7799 and the kit order number is 9125A (134.80) Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From d-heiferman <@t> northwestern.edu Wed Jun 10 13:37:23 2009 From: d-heiferman <@t> northwestern.edu (Daniel Heiferman) Date: Wed Jun 10 13:37:31 2009 Subject: [Histonet] Cresyl Violet Message-ID: We are trying to stain 35 and 5 micron paraffin embedded sections of mouse brain (fixed in 4% paraformaldehyde) using cresyl violet to visualize cell bodies and are having problems achieving a sufficient stain intensity (too light). We are using cresyl violet acetate from Sigma and we have tried using two different stain recipes: 2% cresyl violet acetate in a 0.4M acetate buffer solution at pH = 3.7-3.9. Heated to dissolve then cooled and filtered before use. 0.1% cresyl violet acetate in a 0.1 M acetate buffer solution at pH = 3.6. Stored in the fridge and filtered before use. Between these two recipes, the 0.1% solution had a lot more precipitate that was left behind during filtering as compared to the 2%, which had some but less. This may have been due to the lack of heating, but there was no heating in the 0.1% protocol. We used a standard deparaffination and hydration technique to stain. We tried multiple bath lengths in xylene from 5 minutes to 2 hours, then we put the samples down an ethanol gradient from 95% to 70% to 50% to water. Then we tried different lengths of time for staining, from 2 minutes to 45 minutes, but the stain almost completely washed out on the rehydration steps up the ethanol gradient. Also 70% ethanol on the up gradient has an acetic acid concentration of 1%. Does anyone have any suggestions for improving our protocol? We?re unsure if we need to change cresyl violet concentration, pH, dehydration procedure or something else. Any suggests are greatly appreciated. Thank you From j_mcanally <@t> hotmail.com Wed Jun 10 15:14:53 2009 From: j_mcanally <@t> hotmail.com (Jennifer McAnally) Date: Wed Jun 10 15:14:58 2009 Subject: [Histonet] Job Opening In Long Island, NY Message-ID: Hello Histonetters, I have an opening for a Histology Lab Manager in Long Island, NY. Please review the job description below. If you know someone qualified for this position, please forward this information. For anyone interested, please forward your resume to jennifer@phcconsulting.com for consideration. We are looking for an experienced Histology Lab manager for the Long Island area, relo package available for the right person. This Lab doesn't believe in hierarchies, it promotes personal development, offers flexible schedules, has state of the art equipment and is located in a beautiful setting. This person will be accountable for the direct supervision and operation of the lab and staff. We need someone who can support the company's growth oriented business plan and has an entrepreneurial spirit. This person will be vital to the overall success of the lab so the following characteristics are necessary: Great leadership skills. Ability to train others and compose training manuals Able to smoothly manage the workflow of the lab. Great attitude, energetic and pleasant personality. Knowledge of CAP, State and federal guidelines and regulations pertinent to laboratory safety and security and HIPAA regulations. The ideal candidate has around 4 or more years of laboratory training with 2 years supervisory experience. H.T. or H.T.L. certification and must have qualification to obtain NYS Clinical Laboratory Technologist certificate. No travel required. Must have a great personality, be a team player and have excellent customer service skills. Bi-lingual a big plus! Salary range is $80k to $100k depending on experience. full benefits, healthcare, health savings accounts, 401k and accrued vacation. Jennifer McAnally PHC Consulting 888-263-5688 ext 300 From macveigh <@t> usc.edu Wed Jun 10 17:46:25 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Jun 10 17:46:15 2009 Subject: [Histonet] Working with fat Message-ID: <004601c9ea1d$495024b0$5c237d80@DFS66DD1> Hi all, I used to postfix in PenFix the fat specimens and had good luck. This time we don't have any PenFix :-( The fat is floating in 10% NBF. What can I do to help the fixation, so I can infiltrate well enough, so I can cut the fat and get good morphology? Any suggestion is welcome Michelle USC Keck School of Medicine From esther.peters <@t> verizon.net Wed Jun 10 19:44:35 2009 From: esther.peters <@t> verizon.net (Esther Peters) Date: Wed Jun 10 19:44:45 2009 Subject: [Histonet] Knife strop photo? Message-ID: <4A305373.5080300@verizon.net> Does anyone have a photograph showing the use of an old steel knife leather sharpening sharp? Esther Peters George Mason University From nfournier <@t> sasktel.net Wed Jun 10 20:17:46 2009 From: nfournier <@t> sasktel.net (Neil M. Fournier) Date: Wed Jun 10 20:17:50 2009 Subject: [Histonet] sources for normal goat sera Message-ID: <17BB7DD115E94CD0B74A86B0FD5E3327@Neil45EAF11E9E> Does anyone know of an inexpensive source to purchase normal goat sera for blocking applications during IHC? In the past I purchased normal goat serum through vector. However, it is extremely expensive and they do not provide very much (We go through a lot). Also, I am not sure if there are specific requirements that the sera should have when making a decision on what type would be most optimal for IHC. Any help would be appreciated Thanks Neil E-mail message checked by Spyware Doctor (6.0.1.441) Database version: 6.12580 http://www.pctools.com/en/spyware-doctor-antivirus/ From anh2006 <@t> med.cornell.edu Wed Jun 10 20:21:37 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Jun 10 20:22:46 2009 Subject: [Histonet] sources for normal goat sera Message-ID: <292445207-1244683359-cardhu_decombobulator_blackberry.rim.net-597041197-@bxe1213.bisx.prod.on.blackberry> Gibco who is now with Invitrogen sells it by 500 ml volumes. Jackson Immunoresearch also sells very high quality stuff but more expensive. ------Original Message------ From: Neil M. Fournier Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Jun 10, 2009 9:17 PM Subject: [Histonet] sources for normal goat sera Does anyone know of an inexpensive source to purchase normal goat sera for blocking applications during IHC? In the past I purchased normal goat serum through vector. However, it is extremely expensive and they do not provide very much (We go through a lot). Also, I am not sure if there are specific requirements that the sera should have when making a decision on what type would be most optimal for IHC. Any help would be appreciated Thanks Neil E-mail message checked by Spyware Doctor (6.0.1.441) Database version: 6.12580 http://www.pctools.com/en/spyware-doctor-antivirus/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed Jun 10 20:37:06 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Jun 10 20:37:12 2009 Subject: [Histonet] Knife strop photo? In-Reply-To: <4A305373.5080300@verizon.net> References: <4A305373.5080300@verizon.net> Message-ID: no pics but have 2 strops hanging in my office - interesting conversation pieces maybe you can google it - should be a pic somewhere on the web Annie 2009/6/11 Esther Peters > Does anyone have a photograph showing the use of an old steel knife leather > sharpening sharp? > > Esther Peters > George Mason University > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From JWeems <@t> sjha.org Wed Jun 10 20:38:35 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jun 10 20:38:39 2009 Subject: [Histonet] GMS Control Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5590749@ITSSSXM01V6.one.ads.che.org> Anyone have any non-Aspergillus control block you would share with us? We surely would appreciate it! Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From b-frederick <@t> northwestern.edu Thu Jun 11 07:35:27 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 11 07:35:39 2009 Subject: [Histonet] Knife strop photo? In-Reply-To: Message-ID: <23E079663E8545289EB697EDF13FCB5A@lurie.northwestern.edu> There's a picture in Anne Preece if you have one around. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, June 10, 2009 8:37 PM To: Esther Peters Cc: Histonet Subject: Re: [Histonet] Knife strop photo? no pics but have 2 strops hanging in my office - interesting conversation pieces maybe you can google it - should be a pic somewhere on the web Annie 2009/6/11 Esther Peters > Does anyone have a photograph showing the use of an old steel knife leather > sharpening sharp? > > Esther Peters > George Mason University > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> burnham.org Thu Jun 11 09:00:38 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Thu Jun 11 09:00:55 2009 Subject: [Histonet] Hif-1 alpha Message-ID: I am in need of some help with Hif-1 alpha that was purchased through Novus. The tissue I am working with is mouse breast cancer and mouse lung cancer and the tissue is frozen and fixed in acetone for 10 mins. I am not getting any specific staining because my Negative Control looks just like my Positive Control tissue. I have tried no treatment, Protease 2, pH 8.4 with heat and no heat through the process on a Ventana Discovery XT. I need some one's protocol or recommendations as to how I should proceed with additional optimizing. Thanks!! John From sbreeden <@t> nmda.nmsu.edu Thu Jun 11 09:15:07 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jun 11 09:15:13 2009 Subject: [Histonet] Equipment Insurance? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E468C1@nmdamailsvr.nmda.ad.nmsu.edu> In preparation for our move to a new building (YAHOO!), the question of insurance has arisen its ugly head. I have to assume here that the movers have coverage but details are being confirmed. I have a mental picture of my ASP300 rolling off the moving truck and missing the ramp and ending up... well, you get the picture. Do your labs/facilities carry insurance on equipment? Not just for moving, but for fire, flood and the other usual disasters? I admit to being a Lab Equipment Insurance Virgin J Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From algranth <@t> email.arizona.edu Thu Jun 11 09:32:38 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Jun 11 09:32:46 2009 Subject: [Histonet] Knife strop photo? In-Reply-To: <4A305373.5080300@verizon.net> References: <4A305373.5080300@verizon.net> Message-ID: <877CB9A9-34A9-4451-B4F1-3D383C7E68F3@email.arizona.edu> There is a diagram in the 3rd edition of Preece and also in the green edition of the AFIP book. But if you need an actual photo I could re- enact the technique here and take a picture and email it to you. I still have the strop and some real knives (for show and tell only!). Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Jun 10, 2009, at 5:44 PM, Esther Peters wrote: > Does anyone have a photograph showing the use of an old steel knife > leather sharpening sharp? > > Esther Peters > George Mason University > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From froyer <@t> bitstream.net Thu Jun 11 09:40:13 2009 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Jun 11 09:40:21 2009 Subject: [Histonet] Equipment Insurance? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E468C1@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E468C1@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <2D5F029EAEB042418F566E4AD62718D8@Ford> Whenever you are having equipment moved, always insure it (with the mover) for its full replacement cost. Talk with the moving company that will be doing the work and secure insurance from them to cover the cost of the equipment. If they are unable or unwilling to provide coverage for the dollar amount that you are asking, you might want to look to a different moving company. The national companies... United Van, Mayflower, Allied, Bekins, etc... can provide this coverage. It will add to the cost of the move, but it is well worth it. It would also be helpful to speak with the insurance agent that carries the insurance on your building for their advice. Ford M. Royer Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, June 11, 2009 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Insurance? In preparation for our move to a new building (YAHOO!), the question of insurance has arisen its ugly head. I have to assume here that the movers have coverage but details are being confirmed. I have a mental picture of my ASP300 rolling off the moving truck and missing the ramp and ending up... well, you get the picture. Do your labs/facilities carry insurance on equipment? Not just for moving, but for fire, flood and the other usual disasters? I admit to being a Lab Equipment Insurance Virgin J Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Jun 11 09:42:24 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jun 11 09:42:29 2009 Subject: [Histonet] sources for normal goat sera In-Reply-To: <292445207-1244683359-cardhu_decombobulator_blackberry.rim.net-597041197-@bxe1213.bisx.prod.on.blackberry> References: <292445207-1244683359-cardhu_decombobulator_blackberry.rim.net-597041197-@bxe1213.bisx.prod.on.blackberry> Message-ID: Equitech Bio sells it very very cheap and it's always worked for me. I haven't used serum from Invitrogen or Jackson in years because they're just too expensive. It's about $50 for 500 ml! They have a minimum order of $50 though If you want it heat inactivated, you need to tell them, I think they charge less than five dollars to do it. www.equitechbio.com Emily "Canned food is dead food....here kids can't study. They're going blind. Mothers are out in pants showing themselves off when they ought to be at home cooking fresh vegetables." --Charles Atlas, Saga magazine interview, 1964 From talulahgosh <@t> gmail.com Thu Jun 11 09:43:27 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jun 11 09:43:34 2009 Subject: [Histonet] sources for normal goat sera In-Reply-To: References: <292445207-1244683359-cardhu_decombobulator_blackberry.rim.net-597041197-@bxe1213.bisx.prod.on.blackberry> Message-ID: Sorry, the site is http://www.equitech-bio.com/ not the first one I sent. Emily "Canned food is dead food....here kids can't study. They're going blind. Mothers are out in pants showing themselves off when they ought to be at home cooking fresh vegetables." --Charles Atlas, Saga magazine interview, 1964 From JWeems <@t> sjha.org Thu Jun 11 10:32:19 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jun 11 10:32:23 2009 Subject: [Histonet] Knife strop photo? In-Reply-To: <23E079663E8545289EB697EDF13FCB5A@lurie.northwestern.edu> References: <23E079663E8545289EB697EDF13FCB5A@lurie.northwestern.edu> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA55DF7B1@ITSSSXM01V6.one.ads.che.org> Isn't it so sad that the young ones don't have the opportunity to cut off digits and large slices of tissue and make gashes all the way to the bone?????? Ah the good old days... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, June 11, 2009 8:35 AM To: 'Anne van Binsbergen'; 'Esther Peters' Cc: 'Histonet' Subject: RE: [Histonet] Knife strop photo? There's a picture in Anne Preece if you have one around. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, June 10, 2009 8:37 PM To: Esther Peters Cc: Histonet Subject: Re: [Histonet] Knife strop photo? no pics but have 2 strops hanging in my office - interesting conversation pieces maybe you can google it - should be a pic somewhere on the web Annie 2009/6/11 Esther Peters > Does anyone have a photograph showing the use of an old steel knife leather > sharpening sharp? > > Esther Peters > George Mason University > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From gentras <@t> vetmed.auburn.edu Thu Jun 11 10:55:02 2009 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Jun 11 10:55:08 2009 Subject: [Histonet] Re: Cynthia Favara Message-ID: <4A3128D6.9010005@vetmed.auburn.edu> hello will someone please provide me with contact info for Cynthia Favara ASAP? I need to consult with her on a histonet archive post. Thanks! Atoska From rsrichmond <@t> gmail.com Thu Jun 11 12:39:32 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jun 11 12:39:37 2009 Subject: [Histonet] Re: Knife strop photo? Message-ID: Esther Peters requests a picture of the stropping and honing technique once used (long before this geezer's time) to sharpen microtome blades. Here's a scan, with captions, on my Flickr Web site at http://www.flickr.com/photos/bobrichmond/3616614043/ This is probably out of copyright. I can supply a higher definition scan if you need one for printing purposes. Bob Richmond Samurai Pathologist Knoxville TN From burch007 <@t> mc.duke.edu Thu Jun 11 12:47:47 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Jun 11 12:47:54 2009 Subject: [Histonet] Re: Knife strop photo? In-Reply-To: Message-ID: I sent her a digital image of one we have here at our little hospital. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 06/11/2009 01:40 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Knife strop photo? Esther Peters requests a picture of the stropping and honing technique once used (long before this geezer's time) to sharpen microtome blades. Here's a scan, with captions, on my Flickr Web site at http://www.flickr.com/photos/bobrichmond/3616614043/ This is probably out of copyright. I can supply a higher definition scan if you need one for printing purposes. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gliuygao <@t> hotmail.com Thu Jun 11 13:45:22 2009 From: gliuygao <@t> hotmail.com (yan gao) Date: Thu Jun 11 13:45:26 2009 Subject: [Histonet] Flt3 antibody Message-ID: Hi, Histonet. Anyone has experience in FLT3 antibody on IHC? Thanks Yan Gao HTL (ASCP) _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From macveigh <@t> usc.edu Thu Jun 11 14:26:38 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Jun 11 14:26:25 2009 Subject: [Histonet] Re: Working with fat Message-ID: <001901c9eaca$8a7ad950$5c237d80@DFS66DD1> Hi Cheri, Thank you very much for the formula. I will give it a try. Do you just put the fat into it or it is a post fix? After the hour into this solution, what do you do? Do you move it to NBF or 70% ETOH until you start the processor? How long can the fat stay in this fixative? This is a research project and it is just fat that this group is looking at. Thank you for your help Michelle From macveigh <@t> usc.edu Thu Jun 11 14:34:03 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Jun 11 14:33:51 2009 Subject: [Histonet] Re: Working with fat Message-ID: <004201c9eacb$93f8da30$5c237d80@DFS66DD1> Thank you very much! This sounds perfect! Michelle From CIngles <@t> uwhealth.org Thu Jun 11 14:43:36 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Jun 11 14:45:10 2009 Subject: [Histonet] Knife strop photo? References: <23E079663E8545289EB697EDF13FCB5A@lurie.northwestern.edu> <5D64396A0D4A5346BEBC759022AAEAA55DF7B1@ITSSSXM01V6.one.ads.che.org> Message-ID: I don't know about that... Have you tried one of the automated microtomes? Lets just say I have REALLY quick reflexes now. :) Don't have to siphon by mouth anymore though. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Thu 6/11/2009 10:32 AM To: Bernice Frederick; Anne van Binsbergen; Esther Peters Cc: Histonet Subject: RE: [Histonet] Knife strop photo? Isn't it so sad that the young ones don't have the opportunity to cut off digits and large slices of tissue and make gashes all the way to the bone?????? From amylee779 <@t> yahoo.com Thu Jun 11 17:16:20 2009 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Jun 11 17:16:23 2009 Subject: [Histonet] table for microscope Message-ID: <488954.19421.qm@web38006.mail.mud.yahoo.com> Hello, ? I am looking for a microscope table. It doesn't has to be designed specific for microscope. The microscope I am using is normal sized Nikon E800. I have a computer sit besides. Do you know where I can look? ? Thanks, Amy From woair <@t> aol.com Thu Jun 11 18:53:24 2009 From: woair <@t> aol.com (woair@aol.com) Date: Thu Jun 11 18:54:04 2009 Subject: [Histonet] position available at Bassett Hospital in Cooperstown New York Message-ID: <8CBB906F16BA31C-96C-92B@webmail-mh06.sysops.aol.com> Bassett Hospital in Cooperstown NY has a full time histotechnician position available. Hours are 7:30 am to 4 pm Monday through Friday. Responsibilities include routine histology duties of a full service hospital laboratory. A NYS license as a histotechnician or certified lab technician is required or must be eligible. For more information or if you are interested in the position: Go to http://www.bassett.org/ and click on career center ? Victoria Spoon Anatomic Pathology Manager Bassett Hospital Cooperstown NY 13326 victoria.spoon@bassett.org Tel(607)547-6357 Fax(607)547-3203 Bassett Healthcare, a magnet designated network of four hospitals and 20+ health centers, offers the finest in medical care to the residents of rural, Central New York.? We provide primary and specialty care services to rural communities spanning eight counties.? Bassett has evolved from a community hospital to a nationally renowned academic referral center, which employs almost 3,000 in its research and teaching system, and is affiliated with Columbia University. Bassett Healthcare is located in Cooperstown New York, a picturesque lakeside village that offers year round cultural and recreational opportunities and excellent schools. Cooperstown is a great place to raise your family, make friends, and enjoy a superb quality of life! From dreynold <@t> mdanderson.org Fri Jun 12 08:32:07 2009 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Fri Jun 12 08:32:10 2009 Subject: [Histonet] Re: F4/80 mouse paraffins Message-ID: <785BBF0C5F49CE41BA74460A43A08F0214492216DF@DCPWVMBXC0VS3.mdanderson.edu> We have used rat anti-mouse F4/80 from Cell Sciences, clone BM8 on mouse FFPE tissue successful. We used a 1:50 dilution and Proteniase K (Dako) for antigen retrieval. Our secondary was BioCare Medical's 4+ biotinylated detection system. It did take a longer than normal development time in the DAB but labeling was very nice. You could give this a try with your antibody. Good luck. Donna Reynolds, Chief Histology Laboratory Cancer Biology, SRB 1.660 713-792-8106 From rcharles <@t> state.pa.us Fri Jun 12 09:27:01 2009 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Jun 12 09:27:06 2009 Subject: [Histonet] Removing diff quick stain Message-ID: <3809C163DC1DA54AA534B3C7794D07B639F95AE79C@ENHBGMBX01.PA.LCL> Hello all, I've been asked if there is a way to remove a diff quick stain from a blood smear so as to scrap off the remaining cells for a PCR test. Any ideas in histoland? thanks so much, Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! From akemiat3377 <@t> yahoo.com Fri Jun 12 10:00:59 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jun 12 10:01:03 2009 Subject: [Histonet] Cell phone numbers go public next month Message-ID: <906888.41554.qm@web31304.mail.mud.yahoo.com> Hi All, Happy Friday!? This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. ? All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. From carrolpb <@t> umdnj.edu Fri Jun 12 10:06:59 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jun 12 10:07:08 2009 Subject: [Histonet] Cell phone numbers go public next month In-Reply-To: <906888.41554.qm@web31304.mail.mud.yahoo.com> References: <906888.41554.qm@web31304.mail.mud.yahoo.com> Message-ID: <4A326F13.8060309@umdnj.edu> This is false information. I really wish people would take half a second to verify the false rumors they're helping spread around the internet before sending this tripe to everyone else... especially as an off-topic post spammed to a public mailing-list. Read more about the truth behind this rumor: http://www.snopes.com/politics/business/cell411.asp Akemi Allison-Tacha wrote: > Hi All, > > Happy Friday! This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > Histology Manager > Associated Pathology Medical Group Laboratories > 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 > Cell: 408.335.9994 > W: E-Mail: aallison-tacha@apmglab.com > P: E-Mail: akemiat3377@yahoo.com > > FYI > Folks! > REMEMBER: > Cell Phone Numbers Go Public next month. > All > cell phone numbers are being released to telemarketing companies and > you will start to receive sales calls. > > YOU WILL BE CHARGED > FOR THESE CALLS > Even if > the message is saved on your phone, you will be charged for the > minutes to listen to it. > To > prevent this, call the following number from your cell phone: > 888-382-1222 > > It is > the National DO NOT CALL list. It will only take a minute of your > time. It blocks > your number for five (5) years. > You must call from the cell > phone number you want to > have blocked. You cannot call from a > different phone number. > > HELP OTHERS BY > PASSING THIS ON TO ALL YOUR FRIENDS. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From vazquezr <@t> ohsu.edu Fri Jun 12 10:07:16 2009 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jun 12 10:07:31 2009 Subject: [Histonet] Cell phone numbers go public next month In-Reply-To: <906888.41554.qm@web31304.mail.mud.yahoo.com> References: <906888.41554.qm@web31304.mail.mud.yahoo.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF0838AFBAC@EX-BE08.ohsu.edu> I don't think so, check Snopes.com, they say that is false. And we had something like this a few months ago at work, found out that it was not true. Robyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, June 12, 2009 8:01 AM To: histonet Subject: [Histonet] Cell phone numbers go public next month Hi All, Happy Friday!? This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. ? All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Jun 12 10:14:25 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jun 12 10:13:16 2009 Subject: [Histonet] Cell phone numbers go public next month In-Reply-To: <906888.41554.qm@web31304.mail.mud.yahoo.com> References: <906888.41554.qm@web31304.mail.mud.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088C7B3083@IBMB7Exchange.digestivespecialists.com> Check out this link about cell phone numbers. http://www.snopes.com/politics/business/cell411.asp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, June 12, 2009 11:01 AM To: histonet Subject: [Histonet] Cell phone numbers go public next month Hi All, Happy Friday!? This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. ? All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Jun 12 10:14:22 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jun 12 10:14:25 2009 Subject: [Histonet] Not False! Cell phone numbers go public next month Message-ID: <262792.35629.qm@web31303.mail.mud.yahoo.com> Hi All, This is not false information!!!!? I just called the "government number" from my cell and registered. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com --- On Fri, 6/12/09, Peter Carroll wrote: From: Peter Carroll Subject: Re: [Histonet] Cell phone numbers go public next month To: "Akemi Allison-Tacha" Cc: "histonet" Date: Friday, June 12, 2009, 8:06 AM This is false information. I really wish people would take half a second to verify the false rumors they're helping spread around the internet before sending this tripe to everyone else... especially as an off-topic post spammed to a public mailing-list. Read more about the truth behind this rumor: http://www.snopes.com/politics/business/cell411.asp Akemi Allison-Tacha wrote: > Hi All, > > Happy Friday!? This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > Histology Manager > Associated Pathology Medical Group Laboratories > 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 > Cell: 408.335.9994 W: E-Mail: aallison-tacha@apmglab.com > P: E-Mail: akemiat3377@yahoo.com > > FYI >? ? ? ? ? ???Folks! >? ? ? ? ? ???REMEMBER: >? ? ? ? ? ???Cell Phone Numbers Go Public next month.? ? ? ? ? ? ???All >? ? ? ? ? ???cell phone numbers are being released to telemarketing companies and >? ? ? ? ? ???you will start to receive sales calls. > > YOU WILL BE CHARGED >? ? ? ? ? ???FOR THESE CALLS? ? ? ? ? ???Even if >? ? ? ? ? ???the message is saved on your phone, you will be charged for the >? ? ? ? ? ???minutes to listen to it.? ? ? ? ? ???To >? ? ? ? ? ???prevent this, call the following number from your cell phone: >? ? ? ? ? ???888-382-1222 >? ? ? ? ? ? ? ? ? ? ? ???It is >? ? ? ? ? ???the National DO NOT CALL list. It will only take a minute of your >? ? ? ? ? ???time. It blocks your number for five (5) years. >? ? ? ? ? ???You must call from the cell >? ? ? ? ? ???phone number you want to have blocked. You cannot call from a >? ? ? ? ? ???different phone number. > > HELP OTHERS BY >? ? ? ? ? ???PASSING THIS ON TO ALL YOUR FRIENDS. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >??? From jmcgough <@t> clinlab.com Fri Jun 12 10:19:55 2009 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Fri Jun 12 10:20:04 2009 Subject: [Histonet] Not False! Cell phone numbers go public next month In-Reply-To: <262792.35629.qm@web31303.mail.mud.yahoo.com> Message-ID: You can register your cell phone but there is no risk of your number going to telemarketers if you don't. If you go to www.snopes.com you can read more about it. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi Allison-Tacha Sent: Friday, June 12, 2009 9:14 AM To: Peter Carroll Cc: histonet Subject: Re: [Histonet] Not False! Cell phone numbers go public next month Hi All, This is not false information!!!!? I just called the "government number" from my cell and registered. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com --- On Fri, 6/12/09, Peter Carroll wrote: From: Peter Carroll Subject: Re: [Histonet] Cell phone numbers go public next month To: "Akemi Allison-Tacha" Cc: "histonet" Date: Friday, June 12, 2009, 8:06 AM This is false information. I really wish people would take half a second to verify the false rumors they're helping spread around the internet before sending this tripe to everyone else... especially as an off-topic post spammed to a public mailing-list. Read more about the truth behind this rumor: http://www.snopes.com/politics/business/cell411.asp Akemi Allison-Tacha wrote: > Hi All, > > Happy Friday!? This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > Histology Manager > Associated Pathology Medical Group Laboratories > 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 > Cell: 408.335.9994 W: E-Mail: aallison-tacha@apmglab.com > P: E-Mail: akemiat3377@yahoo.com > > FYI >? ? ? ? ? ???Folks! >? ? ? ? ? ???REMEMBER: >? ? ? ? ? ???Cell Phone Numbers Go Public next month.? ? ? ? ? ? ???All >? ? ? ? ? ???cell phone numbers are being released to telemarketing companies and >? ? ? ? ? ???you will start to receive sales calls. > > YOU WILL BE CHARGED >? ? ? ? ? ???FOR THESE CALLS? ? ? ? ? ???Even if >? ? ? ? ? ???the message is saved on your phone, you will be charged for the >? ? ? ? ? ???minutes to listen to it.? ? ? ? ? ???To >? ? ? ? ? ???prevent this, call the following number from your cell phone: >? ? ? ? ? ???888-382-1222 >? ? ? ? ? ? ? ? ? ? ? ???It is >? ? ? ? ? ???the National DO NOT CALL list. It will only take a minute of your >? ? ? ? ? ???time. It blocks your number for five (5) years. >? ? ? ? ? ???You must call from the cell >? ? ? ? ? ???phone number you want to have blocked. You cannot call from a >? ? ? ? ? ???different phone number. > > HELP OTHERS BY >? ? ? ? ? ???PASSING THIS ON TO ALL YOUR FRIENDS. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Jun 12 10:22:08 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jun 12 10:22:15 2009 Subject: [Histonet] Not False! Cell phone numbers go public next month In-Reply-To: <262792.35629.qm@web31303.mail.mud.yahoo.com> References: <262792.35629.qm@web31303.mail.mud.yahoo.com> Message-ID: <4A3272A0.9060000@umdnj.edu> Yes, it is false. I'm afraid you've confused the toll-free number for the long-existent FCC's "Do Not Call Registry" with some other magical "government number". Here is another link indicating that your "warning" is a persistent email hoax: http://urbanlegends.about.com/od/business/a/cell_directory.htm Akemi Allison-Tacha wrote: > Hi All, > > This is not false information!!!! I just called the "government > number" from my cell and registered. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > Histology Manager > Associated Pathology Medical Group Laboratories > 105A Cooper Ct. Los Gatos, CA 95032 > Direct: 408.884.2718 > Cell: 408.335.9994 > W: E-Mail: aallison-tacha@apmglab.com > P: E-Mail: akemiat3377@yahoo.com > > > > --- On *Fri, 6/12/09, Peter Carroll //* wrote: > > > From: Peter Carroll > Subject: Re: [Histonet] Cell phone numbers go public next month > To: "Akemi Allison-Tacha" > Cc: "histonet" > Date: Friday, June 12, 2009, 8:06 AM > > This is false information. > > I really wish people would take half a second to verify the false > rumors they're helping spread around the internet before sending > this tripe to everyone else... especially as an off-topic post > spammed to a public mailing-list. > > Read more about the truth behind this rumor: > http://www.snopes.com/politics/business/cell411.asp > > > > Akemi Allison-Tacha wrote: > > Hi All, > > > > Happy Friday! This is a little off the histology subject, but > for those of you who have cell phones, might find this beneficial. > > > > Akemi Allison-Tacha BS, HT (ASCP) HTL > > Histology Manager > > Associated Pathology Medical Group Laboratories > > 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 > > Cell: 408.335.9994 W: E-Mail: aallison-tacha@apmglab.com > > > P: E-Mail: akemiat3377@yahoo.com > > > > > FYI > > Folks! > > REMEMBER: > > Cell Phone Numbers Go Public next month. > All > > cell phone numbers are being released to > telemarketing companies and > > you will start to receive sales calls. > > > > YOU WILL BE CHARGED > > FOR THESE CALLS Even if > > the message is saved on your phone, you will be > charged for the > > minutes to listen to it. To > > prevent this, call the following number from your > cell phone: > > 888-382-1222 > > It is > > the National DO NOT CALL list. It will only take a > minute of your > > time. It blocks your number for five (5) years. > > You must call from the cell > > phone number you want to have blocked. You cannot > call from a > > different phone number. > > > > HELP OTHERS BY > > PASSING THIS ON TO ALL YOUR FRIENDS. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > From akemiat3377 <@t> yahoo.com Fri Jun 12 10:22:33 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jun 12 10:22:41 2009 Subject: [Histonet] form for internal and external errors and corrective actions. Message-ID: <109350.41596.qm@web31303.mail.mud.yahoo.com> Hi All, I am updating several of our lab forms, and we do not have a form for internal and external errors and corrective actions.? As you well know, this is a CAP requirement.? Could any of you share your forms with me? Thanks a bunch, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com From stella_quan <@t> dnar.com Fri Jun 12 11:16:24 2009 From: stella_quan <@t> dnar.com (Stella Quan) Date: Fri Jun 12 11:20:48 2009 Subject: [Histonet] Histotechnologist Job Posting Message-ID: <49110CB9C6A2FD4B9E9AA5FBC6E9F0B90966D08A1A@MBX75.ad2.softcom.biz> Dear Histonet Coordinator, I really appreciate your efforts in maintaining such a great web site. Please kindly post my job posting on our web site. "Come and Join DNAR! DNAR is a fast-growing biotechnology company creating innovative cancer diagnostics to meet the need for better informing patients and their doctors about treatment choices in their cancer care. DNAR is located in Greater Boston area, Massachusetts. DNAR is currently hiring talented histotechnologists with extensive IHC experiences to work in a team for successful IHC assay development for cancer markers. We offer competitive salary, benefits and stock options. To learn more about our company, please visit our website: www.dnar.com. If you are interested in pursuing this position, please send your resume to: jobs@dnar.com with the Code: DN571. " Thanks very much for your help. Please do not hesitate to contact me. Have a nice day! Sincerely, Stella Quan, Ph.D. DNAR Senior Director of Product Development 1 Kendall Square, Building 300, 3rd floor Cambridge, MA 02139 617-517-3210 x115 From enrriq88 <@t> yahoo.com Fri Jun 12 13:08:39 2009 From: enrriq88 <@t> yahoo.com (Jaime Plata) Date: Fri Jun 12 13:08:42 2009 Subject: [Histonet] Grossing techniques Message-ID: <297547.10592.qm@web50407.mail.re2.yahoo.com> Hello Fellows, Anybody knows or recommend a book or manual for grossing tissue techniques ? please let me know. I am looking for a more practical one on? gross description. Thanks you all Jaime E Plata MT.HTL (ASCP) enrriq88@yahoo.com From leiker <@t> buffalo.edu Fri Jun 12 14:38:14 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Jun 12 14:38:23 2009 Subject: [Histonet] C-kit (cd117) antibody Message-ID: <3BD1FBA5828DAD3FC030EDB9@CDYwxp1931.ad.med.buffalo.edu> I'm looking for a good c-kit antibody that's either made against or cross-reacts with rat. Any ideas out there? Specifically I'd like to use it for immunofluorescent staining on frozen tissue. Thanks! Merced M Leiker Research Technician II Cardiovascular Medicine 361 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From KGroeger <@t> USLABS.net Fri Jun 12 15:10:35 2009 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Fri Jun 12 15:10:40 2009 Subject: [Histonet] Grossing techniques In-Reply-To: <297547.10592.qm@web50407.mail.re2.yahoo.com> References: <297547.10592.qm@web50407.mail.re2.yahoo.com> Message-ID: Surgical Pathology Dissection (Second Edition) Written by William Westra, Ralph Hruban, Timothy Phelps and Christian Isacson a good guide with a lot of illustrations. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaime Plata Sent: Friday, June 12, 2009 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing techniques Hello Fellows, Anybody knows or recommend a book or manual for grossing tissue techniques ? please let me know. I am looking for a more practical one on gross description. Thanks you all Jaime E Plata MT.HTL (ASCP) enrriq88@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From esther.peters <@t> verizon.net Fri Jun 12 15:46:27 2009 From: esther.peters <@t> verizon.net (Esther Peters) Date: Fri Jun 12 15:55:13 2009 Subject: [Histonet] Re: Knife strop photo? In-Reply-To: <4A305373.5080300@verizon.net> References: <4A305373.5080300@verizon.net> Message-ID: <4A32BEA3.6050500@verizon.net> Thank you to everyone who provided us with information, illustrations, and photography resources on this topic! Esther Esther Peters wrote: > Does anyone have a photograph showing the use of an old steel knife > leather sharpening sharp? > > Esther Peters > George Mason University > > From sbruce <@t> vetpathservicesinc.com Fri Jun 12 17:37:28 2009 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Fri Jun 12 17:38:05 2009 Subject: [Histonet] RE: Cell phones Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A28552A6@vpss1.VetPathServicesInc.local> The cell phone do not call list is real. In Ohio several people I know started getting lots of 'spam' calls on their cell phones. They called their carrier (verizon) and asked how to stop it. They recommended the government do not call list. Once they called that number the spamming stopped. If you read the snopes website the information stops in 2006. Call your cell phone carrier if you are concerned instead of checking the internet if you are receiving these annoying calls. Suzanne From jnocito <@t> satx.rr.com Fri Jun 12 17:55:21 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jun 12 17:55:32 2009 Subject: [Histonet] Grossing techniques In-Reply-To: References: <297547.10592.qm@web50407.mail.re2.yahoo.com> Message-ID: <84EE0930EC4E47AE82265F3534348627@JoePC> a.. Manual of Surgical Pathology by Susan Lester b.. c.. Publisher: Saunders W B Co d.. Pub. Date: October 2005 e.. ISBN-13: 9780443066450 f.. Sales Rank: 56,744 g.. 384pp h.. Edition Description: REV i.. Edition Number: 2 We use this one. It has good pictures with samples of gross descriptions. I wish I knew about this book before I took my PA exam. Joe Nocito, BS, PA, HT (ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Karin Groeger" To: "Jaime Plata" ; Sent: Friday, June 12, 2009 3:10 PM Subject: RE: [Histonet] Grossing techniques Surgical Pathology Dissection (Second Edition) Written by William Westra, Ralph Hruban, Timothy Phelps and Christian Isacson a good guide with a lot of illustrations. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaime Plata Sent: Friday, June 12, 2009 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing techniques Hello Fellows, Anybody knows or recommend a book or manual for grossing tissue techniques ? please let me know. I am looking for a more practical one on gross description. Thanks you all Jaime E Plata MT.HTL (ASCP) enrriq88@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Fri Jun 12 20:06:04 2009 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Fri Jun 12 20:06:08 2009 Subject: [Histonet] P.A PROGRAMME Message-ID: Hi, Please does any one know of any medical facility or any medical centers who is interested in training qualified techs to become a P.A. I will appreciate it, lf you guys could pass the infos of such facility to me. Talk to you soon. Ade _________________________________________________________________ Windows Live?: Keep your life in sync. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_BR_life_in_synch_062009 From jwarren23 <@t> cinci.rr.com Fri Jun 12 10:27:29 2009 From: jwarren23 <@t> cinci.rr.com (Jean Warren) Date: Sat Jun 13 06:41:23 2009 Subject: [Histonet] Cell phone numbers go public next month In-Reply-To: <906888.41554.qm@web31304.mail.mud.yahoo.com> References: <906888.41554.qm@web31304.mail.mud.yahoo.com> Message-ID: <85FF7C41561747DF989B6EB503C7D88B@ownerPC> I do not mean to contradict anyone, but there is a web-page called "Snopes", which is a myth debunking service. Read what they say about the telemarketers and cell phones. I have found "Snopes" to be accurate so far in anything I have checked. They say the following information is false. http://www.snopes.com/politics/business/cell411.asp ----- Original Message ----- From: "Akemi Allison-Tacha" To: "histonet" Sent: Friday, June 12, 2009 11:00 AM Subject: [Histonet] Cell phone numbers go public next month Hi All, Happy Friday! This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From littledip <@t> hotmail.com Sat Jun 13 06:45:16 2009 From: littledip <@t> hotmail.com (littledip@hotmail.com) Date: Sat Jun 13 06:45:36 2009 Subject: [Histonet] Cell phone numbers go public next month In-Reply-To: <85FF7C41561747DF989B6EB503C7D88B@ownerPC> References: <906888.41554.qm@web31304.mail.mud.yahoo.com><85FF7C41561747DF989B6EB503C7D88B@ownerPC> Message-ID: Sent via BlackBerry from T-Mobile -----Original Message----- From: "Jean Warren" Date: Fri, 12 Jun 2009 11:27:29 To: Akemi Allison-Tacha; histonet Subject: Re: [Histonet] Cell phone numbers go public next month I do not mean to contradict anyone, but there is a web-page called "Snopes", which is a myth debunking service. Read what they say about the telemarketers and cell phones. I have found "Snopes" to be accurate so far in anything I have checked. They say the following information is false. http://www.snopes.com/politics/business/cell411.asp ----- Original Message ----- From: "Akemi Allison-Tacha" To: "histonet" Sent: Friday, June 12, 2009 11:00 AM Subject: [Histonet] Cell phone numbers go public next month Hi All, Happy Friday! This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Jun 13 12:42:32 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Jun 13 12:43:15 2009 Subject: [Histonet] Re:C-kit Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0EC4@KCL-MAIL04.kclad.ds.kcl.ac.uk> http://www.immunoportal.com/index.php carl From milton.gomez <@t> aruplab.com Sat Jun 13 15:33:03 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Sat Jun 13 15:33:08 2009 Subject: [Histonet] Gram Control Message-ID: Good afternoon Histonetters: May someone share a gram control block for special stains? May you also share how can I prepare one in my lab? Thanks, Milton - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From rjbuesa <@t> yahoo.com Sat Jun 13 15:36:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jun 13 15:36:51 2009 Subject: [Histonet] Gram Control Message-ID: <602708.43605.qm@web65705.mail.ac4.yahoo.com> Get a human appendix block. Ren? J. --- On Sat, 6/13/09, Gomez, Milton wrote: From: Gomez, Milton Subject: [Histonet] Gram Control To: "Histonet" Date: Saturday, June 13, 2009, 4:33 PM Good afternoon Histonetters: May someone share a gram control block for special stains?? May you also share how can I prepare one in my lab? Thanks, Milton - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From milton.gomez <@t> aruplab.com Sat Jun 13 16:01:26 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Sat Jun 13 16:01:30 2009 Subject: [Histonet] Gram Control Message-ID: Will a normal human appendix demonstrate Gram + and negative bacteria, and also Filaments of Nocardia and Actinomyces? MG - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From enrriq88 <@t> yahoo.com Sun Jun 14 11:23:07 2009 From: enrriq88 <@t> yahoo.com (enrriq88@yahoo.com) Date: Sun Jun 14 11:23:12 2009 Subject: [Histonet] Gram Control Message-ID: <977222.9394.qm@web50405.mail.re2.yahoo.com> actinomycosis and nocardiosis, respectively. Actinomyces israelii are gram-positive bacillary and branching forms that are referred to as "higher" bacteria. These organisms occur as parasites in humans and other animals. In tissues, they form hard "sulfur" granules. They need blood for growth on media. Nocardia asteroides are also gram-positive, filamentous "higher" bacteria. Fragments of hyphae appear as bacilli (rod-shaped bacteria) or cocci (spherical bacteria). Many strains are not easily decolorized by acids (acid-fast). They are often found in soil, and grow well on ordinary media. In addition, the pathophysiology of this microorganism in other to be present in appendix tissue needs either a systemic infection in place or a post-surgical granulomatous reaction occurring at same time. All this blows out any possibility to find them in normal appendix. I hope this help you. ? Jaime E Plata MD.MT.HTL --- On Sat, 6/13/09, Gomez, Milton wrote: From: Gomez, Milton Subject: [Histonet] Gram Control To: "Histonet" Date: Saturday, June 13, 2009, 5:01 PM Will a normal human appendix demonstrate Gram + and negative bacteria, and also Filaments of Nocardia and Actinomyces? MG - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sun Jun 14 12:11:35 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun Jun 14 12:11:39 2009 Subject: [Histonet] Re: Gram control Message-ID: You'd want an appendix that showed acute gangrenous appendicitis, and I'd expect to look at more than one before finding a satisfactory control. Your pathologist needs to help with this. Tissue Gram stains are much less useful than they're supposed to be. They don't have the sensitivity or specificity of Gram stains done on flame-fixed smears. The stains are complex and time-consuming, and some of the older techniques require flammable solvents. If you just want to look for bacteria, use a Giemsa or Diff-Quik II stain. Much simpler, and more sensitive. This technique is supposed to be standard for quantifying bacteria in biopsy specimens of burn victim skin. Bob Richmond Samurai Pathologist Knoxville TN From AnthonyH <@t> chw.edu.au Sun Jun 14 18:03:04 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jun 14 18:03:15 2009 Subject: [Histonet] form for internal and external errors and correctiveactions. In-Reply-To: <109350.41596.qm@web31303.mail.mud.yahoo.com> Message-ID: Akemi, Here in Australia we have a similar requirement. Here is the gist of our form: CORRECTIVE ACTION REQUEST Date: Person making complaint: Time: Person taking complaint: Nature of complaint: Details of complaint: Initial response or corrective action taken: Review of corrective action: Issue raised at management meeting: Y/N Further action required?: Y/N CAR completed?: Y/N Signed: .................................................. Date: .................... Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Saturday, 13 June 2009 1:23 AM To: histonet Subject: [Histonet] form for internal and external errors and correctiveactions. Hi All, I am updating several of our lab forms, and we do not have a form for internal and external errors and corrective actions.? As you well know, this is a CAP requirement.? Could any of you share your forms with me? Thanks a bunch, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994? W: E-Mail: aallison-tacha@apmglab.com P: E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Susan.Walzer <@t> HCAHealthcare.com Mon Jun 15 02:14:11 2009 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Jun 15 02:14:34 2009 Subject: [Histonet] RE: Gram Control In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2ABB45E20B@FWDCWPMSGCMS09.hca.corpad.net> We take some fresh tissue (we use umbilical cord) and have micro incubate half with gram positive and the other with negative. After a few day we fix the tissues and put a piece of each in blocks. We then do a modified gram stain using acetone and the stain always looks great. See below: III. SOLUTIONS: A. 1% Crystal Violet Solution B. 1% Basic fuchsin Solution C. Gram's Iodine Solution ( 1 gm iodine, 2 gm. potassium iodide in 300 ml. distilled water.) D. Gallego's Differentiating Solution: 1. distilled water................100ml 2. Formalin, 37%....................2 ml 3. acetic acid......................1 ml E. Picric Acid-Acetone ( available from Polyscientific #S1867) Acetone- Xylene IV. PROCEDURE: A. Deparaffinize and dehydrate to water. B. Place in 1% crystal violet for 1 min. C. Rinse in tap water. D. Place in Gram's Iodine for 1 min. E. Rinse in tap water. SUBJECT: BROWN AND HOPPS GRAM STAIN PAGE 2 OF 2 F. Decolorize in acetone until background is clear. G. Wash immediately in tap water. H. Place in 1% basic fuchsin 1 min. I. Rinse in tap water. J. Place in Gallego's differentiating solution, 2 changes, 1 minute each. K. Rinse in tap water. L. Place in acetone for 30 seconds. M. Place in picric acid-acetone for 2 minutes. N. Place in acetone-xylene, 2 changes. O. Clear in xylene and coverslip. V. RESULTS: A. Gram-positive bacteria.....................blue B. Gram-negative bacteria......................red C. Background...............................yellow -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton Sent: Saturday, June 13, 2009 4:33 PM To: Histonet Subject: [Histonet] Gram Control Good afternoon Histonetters: May someone share a gram control block for special stains? May you also share how can I prepare one in my lab? Thanks, Milton - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfountain <@t> exchange.hsc.mb.ca Mon Jun 15 10:43:51 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Mon Jun 15 10:43:58 2009 Subject: [Histonet] Synaptophysin Message-ID: Hello, We are currently using a Synaptophysin (clone SY38) from Millipore (Chemicon) and our pathologists are not satisfied with it's performance. We are looking for an IVD Synapto and want to know which companies/clones and protocols people are using.... Also, does everyone use a normal appendix for the control as suggested by NordiQC?? THANKS FOR YOUR HELP!! Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From judith_pardue <@t> memorial.org Mon Jun 15 08:50:10 2009 From: judith_pardue <@t> memorial.org (Pardue, Judith) Date: Mon Jun 15 10:45:33 2009 Subject: [Histonet] Immuno Assessments Message-ID: Has anyone had any experience with an Immuno survey group out of Denmark named NordiQC. Judith Pardue Memorial Health Care System Chattanooga,Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From anh2006 <@t> med.cornell.edu Mon Jun 15 10:51:44 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Jun 15 10:51:56 2009 Subject: [Histonet] CD115/c-fms/CSF-1R Message-ID: Has anyone stained CD115 (aka c-fms/CSF-1R) in mouse tissue? Any suggestions of antibodies and protocols? Thanks, Andrea -- From LSebree <@t> uwhealth.org Mon Jun 15 11:34:02 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Jun 15 11:34:06 2009 Subject: [Histonet] Synaptophysin In-Reply-To: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CE07F@UWHC-MAIL01.uwhis.hosp.wisc.edu> Tiana, We use Ventana's Synaptophysin, clone 4C4.9, and pancreas or a multi-tumor neuro block as the control tissue. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiana Fountain Sent: Monday, June 15, 2009 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Synaptophysin Hello, We are currently using a Synaptophysin (clone SY38) from Millipore (Chemicon) and our pathologists are not satisfied with it's performance. We are looking for an IVD Synapto and want to know which companies/clones and protocols people are using.... Also, does everyone use a normal appendix for the control as suggested by NordiQC?? THANKS FOR YOUR HELP!! Tiana From LSebree <@t> uwhealth.org Mon Jun 15 11:38:19 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Jun 15 11:38:24 2009 Subject: [Histonet] Synaptophysin: OOPS! In-Reply-To: <5998F3BDFF7AAC4091C7AE93A7A1A5892CE07F@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CE080@UWHC-MAIL01.uwhis.hosp.wisc.edu> Sorry I pulled our S100 folder instead of our Synaptophsin folder. We use Cell Marque's polyclonal IVD Synaptophysin with the control tissues stated below. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: Sebree Linda A Sent: Monday, June 15, 2009 11:34 AM To: 'Tiana Fountain'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Synaptophysin Tiana, We use Ventana's Synaptophysin, clone 4C4.9, and pancreas or a multi-tumor neuro block as the control tissue. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiana Fountain Sent: Monday, June 15, 2009 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Synaptophysin Hello, We are currently using a Synaptophysin (clone SY38) from Millipore (Chemicon) and our pathologists are not satisfied with it's performance. We are looking for an IVD Synapto and want to know which companies/clones and protocols people are using.... Also, does everyone use a normal appendix for the control as suggested by NordiQC?? THANKS FOR YOUR HELP!! Tiana From jluis.palazon <@t> icman.csic.es Mon Jun 15 13:04:09 2009 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Mon Jun 15 13:04:15 2009 Subject: [Histonet] paper Message-ID: <643379609.118841245089049466.JavaMail.tomcat@saruman> Dear histo-fellows greetings I would like to know if any of you can please read a paper I wrote about immunohistochemistry of a fish islet. English is not my mother languaje so I would appreciate if any of you (whose mother languaje is english) can read it and make all comments you think necesary for improving both the content and the language. Many thanks in advance Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela email: jluis.palazon@icman.csic.es; jpalazon@ne.udo.edu.ve tlf/Fax: 0295-2913150; cell: 0416-5960174 From MAUGER <@t> email.chop.edu Mon Jun 15 13:52:39 2009 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Mon Jun 15 13:53:03 2009 Subject: [Histonet] Re: research genetics/phoenix biotech In-Reply-To: <643379609.118841245089049466.JavaMail.tomcat@saruman> References: <643379609.118841245089049466.JavaMail.tomcat@saruman> Message-ID: <4A3660370200003100007C32@email.chop.edu> Hi everyone, I am unable to find this company anymore. Does anyone know if old research genetics products are still being sold? I don't want Invitrogen- they did away with the product I want-MicroHyb. Thanks, Jo Mauger From JPeters <@t> bostwicklaboratories.com Mon Jun 15 14:34:33 2009 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Mon Jun 15 14:34:40 2009 Subject: [Histonet] BCL-6 Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F05DFAF54@mail1.BOSTWICK.COM> I am having trouble getting bcl-6 antibody to work (Biocare CM223). Does anyone have a good protocol for me to try? From sbecke2 <@t> emory.edu Mon Jun 15 16:27:02 2009 From: sbecke2 <@t> emory.edu (Samuel Beckerman) Date: Mon Jun 15 16:29:11 2009 Subject: [Histonet] Unable to detect protein tagged to GFP Message-ID: <20090615172702.cy3835vfswo880gk@webmail.service.emory.edu> Greetings- This is a follow up to posts made in February of this year under the heading "[Histonet] expression pattern of GFP fusion protein in cells and mouse" My name is Sam Beckerman. I am currently conducting research at Emory University. Recently, my group has experienced difficulties detecting a GFP-tagged fusion protein in neuronal cells. Specifically, we have made lentivirus that expresses GFP tagged to our protein of interest which we inject into rat CNS. After fixation (4% PFA) and cryosectioning we have preformed preliminary examination for GFP and are able to detect strong GFP signal in our tissue. However, we have been unsuccessful at using antibodies against our protein to co-localize with GFP. We have used this same virus to infect 293 cells and PC12 cells and have been able to colocalize our proteins in vitro. Any help would be much appreciated. From Marilyn.A.Weiss <@t> kp.org Mon Jun 15 18:02:35 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Jun 15 18:04:51 2009 Subject: [Histonet] marilyn weiss will be out of the office Message-ID: I will be out of the office starting 06/15/2009 and will not return until 06/18/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. From jluis.palazon <@t> icman.csic.es Tue Jun 16 01:16:50 2009 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Tue Jun 16 01:16:55 2009 Subject: [Histonet] paper Message-ID: <1166608649.221245133010322.JavaMail.tomcat@saruman> Dear List-fellows Many thanks to all of you that kindly accepted to read my paper. greetings Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela email: jluis.palazon@icman.csic.es; jpalazon@ne.udo.edu.ve tlf/Fax: 0295-2913150; cell: 0416-5960174 From alyssa <@t> alliedsearchpartners.com Tue Jun 16 09:27:23 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Jun 16 09:27:31 2009 Subject: [Histonet] 3 Openings For Histology Professionals Message-ID: 1. *Histotechnologist/Technician St.** Petersburg**, FL-* Shifts Available: Days (8:00-8:30 ? 4:30-5:00) Degrees/Certifications: Graduate of a 2 year program with internship, AS degree Florida licensure Preferred ? BA or BS degree, HT (ASCP) certification Preferred background and skills: Laboratory experience in histology or recent graduate of an approved program including immunohistochemistry Knowledge of histology instrumentation/technology Good communication and excellent customer service skills Proficient computer skills Top reasons a Histotechnologist/technician would want to work in this department: CAP accredited laboratory Highly motivated and experienced staff State of the art instrumentation/technology Great teamwork What are 3 behaviors the manager would most like to see exhibited in the department? Good positive attitude, high motivation, and dependable 2. *Lead **Histotechnologist-Augusta, GA* Status: Exempt. Regular, full-time. Work Shift: Monday-Friday, Day Shift, about 7:30am-3:30pm QUALIFICATIONS ASCP certification required Bachlor?s degree preferred HTL Eligible if not already certified with HTL Senior level histotechs, or histotech with at LEAST 5 years experience, ready for a supervisory role. Salary Range $44,384-$70,561 per year. This salary could go beyond the top of the range under certain circumstances. Relocation Yes, relocation is offered. The amount depends on how far away a qualified candidate will be moving from. 3. *Histotechnologist Lead-Long Island, NY (Stony Brook, NY)* Location: Long Island: Stony Brook, NY 3-5 years experience as a Histotechnologist, with at least 1-2 years in leadership role preferred and knowledge/ experience in immunohistochemistry is a plus. Benefit package includes moving expenses, healthcare coverage, 401K with profit sharing plan, CME expenses and life insurance. Come work with a growing private pathology laboratory in Suffolk County in Long Island, NY established in 1998. We work as a team in a friendly and professional environment to provide highest quality service to our client. We use state of art pathology equipments. We have excellent compensation package based on experience and productivity. Salary matching/very competitive compensation -- Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From k.fd <@t> live.com Tue Jun 16 15:10:06 2009 From: k.fd <@t> live.com (Karen Doty) Date: Tue Jun 16 15:10:11 2009 Subject: [Histonet] PR and ER staining in dog uterus Message-ID: Hi, Folks, We are starting to work on a project that requires PR and ER staining in dog uterus. I'm hoping someone will share some tips such as which antibodies work well in dog (clone, manufacturer), and any specific special procedures that might help, such as which antigen retrieval methods work best. I've tried a couple antibodies already without good results in dog although they worked very well in a mouse control. All suggestions and advice is appreciated. Thanks very much. Karen Doty Dept. of Veterinary Biosciences University of Illinois _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From MSHERWOOD <@t> PARTNERS.ORG Wed Jun 17 08:30:55 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jun 17 08:30:59 2009 Subject: [Histonet] Re: Inactivation of DAB Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From dreynold <@t> mdanderson.org Wed Jun 17 08:33:15 2009 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Wed Jun 17 08:33:43 2009 Subject: [Histonet] RE: Research Genetics In-Reply-To: <546b6807-74bb-4ac5-9853-678ca35f0e45@DCPWPRTR01.mdanderson.edu> References: <546b6807-74bb-4ac5-9853-678ca35f0e45@DCPWPRTR01.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F0214492216E7@DCPWVMBXC0VS3.mdanderson.edu> Research Genetics has merged a couple of time the last few years. They are now Open Biosystems in Huntsville, Al pho 888 412 2225. They do not list the Research Genetics on the Web but last I knew products were still available. You can call them to get current catalog number and prices. Donna Reynolds HT (ASCP) Chief Histology Laboratory U. T. M.D. Cancer Center Cancer Biology Core Immunohisto Lab 713-792-8106 Message: 2 Date: Mon, 15 Jun 2009 14:52:39 -0400 From: "Joanne Mauger" Subject: [Histonet] Re: research genetics/phoenix biotech To: , Message-ID: <4A3660370200003100007C32@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Hi everyone, I am unable to find this company anymore. Does anyone know if old research genetics products are still being sold? I don't want Invitrogen- they did away with the product I want-MicroHyb. Thanks, Jo Mauger ------------------------------ **** From akbitting <@t> geisinger.edu Wed Jun 17 08:50:09 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Jun 17 08:50:25 2009 Subject: [Histonet] Looking for NYU contact Message-ID: <4A38BC51.2B7F.00C9.0@geisinger.edu> Just wondering if anyone can give me contact information for someone in the Neuropath Lab at NYU? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From lpaveli1 <@t> hurleymc.com Wed Jun 17 09:05:13 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jun 17 09:05:32 2009 Subject: [Histonet] Re: Inactivation of DAB Message-ID: <4A38BFD9020000EE0002A178@smtp-gw.hurleymc.com> We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184. 1. Prepare the following aqeous stock solutions: a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter) b. 2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter) 2. Dilute DAB solution of necessary so that its concentration does not exceed 0.9 mg/ml. 3. For each 10ml of DAB solution, add: a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter) b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%) 4. Allow mixture to stand for at least 10 hours. It is now non-mutagenic. 5. Decolorize the mixture with ascorbic acid (add powder until color disappears). This too is an oxidation/reduction reaction. 6. Neutralize the decolorized mixture with sodium bicarbonate (test with pH meter, pH paper or dipsticks). Note: we recommend using magnesium hydroxide/oxide instead of sodium bicarbonate. 7. Discard the solution down the drain provided that local wastewater authorities have given their approval. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Sherwood, Margaret " 06/17/09 9:30 AM >>> We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Wed Jun 17 09:27:39 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Jun 17 09:27:43 2009 Subject: [Histonet] Re: Inactivation of DAB In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> Message-ID: Hi Peggy, You wrote: > We have started doing routine immunohistochemistry on our stainer and have a > large waste container that includes diaminobenzidine (DAB). > > We would like to inactivate it so we don't have to pay to have the waste > removed. Can anyone help me? What do people use to inactivate their DAB? May I suggest that you access the histonet archives where you will be able to see all the previous discussions on this topic. The responses were many and varied depending on different lab types and locations. Good luck with your search. Respectfully, Christie Gowan Surgical Pathology University of Alabama Hospital From Rhonda.Gregoire <@t> gov.mb.ca Wed Jun 17 12:07:55 2009 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRI)) Date: Wed Jun 17 12:09:32 2009 Subject: FW: [Histonet] coverslippers Message-ID: <9A52FA4A0436FC489251BAB5249DAADC03FA2892@OC1EX01.ME.MBGOV.CA> Hi Patsy, We have had the Surgipath coverslipper for 3 years and are really happy with it. We have a smaller Histology section and only do 20-100 slides per day. It works great for us, as long as you keep everything cleaned when there are any coverslips that break. Rhonda Gregoire, R.T. Charge Technologist Clinical Pathology/TSE Section Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Initiatives 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca From Pat.Bell <@t> ucdenver.edu Wed Jun 17 12:35:15 2009 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Jun 17 12:35:46 2009 Subject: [Histonet] RNA In Situ Hybridization Message-ID: <64DB27005E2FD3439E88502D7A5C9121877CB00397@CORTEZ.ucdenver.pvt> I want to learn to do In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have the Dako Autostainer, but I know that some of the procedure would be done manually. I would appreciate any protocols that would help me. Thank you for your help. Pat Pat Bell HT(ASCP) Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From MadaryJ <@t> MedImmune.com Wed Jun 17 13:26:09 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Jun 17 13:26:18 2009 Subject: [Histonet] source for rat anti mouse brdu? Message-ID: I have not done a brdu on mouse in five years. Used Harlan but they are not selling that anymore. I used a procedure using rat anti mouse brdu primary, rabbiot anti rat mouse adsorbed secondary, then ABC/DAB. Anyone have any idea on a good source? I used biocompare and found stuff but would like to hear comments. Also anyone use conjugated brdu? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From susanbachus <@t> verizon.net Wed Jun 17 13:59:09 2009 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Wed Jun 17 13:59:20 2009 Subject: [Histonet] RNA In Situ Hybridization In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121877CB00397@CORTEZ.ucdenver.pvt> References: <64DB27005E2FD3439E88502D7A5C9121877CB00397@CORTEZ.ucdenver.pvt> Message-ID: <424A2F7D71944044BAA16870D3BB1826@OwnerPC> Protocol #3 at this site is great for RNA probes: http://intramural.nimh.nih.gov/lcmr/snge/Protocols/ISHH/ISHH.html This protocol assumes fresh frozen tissue but can be used on fixed, paraffin embedded tissue--obviously you would have to include a step at the beginning to deparaffinize. You might also want to look at HIER methods that can improve results from fixed tissue. ----- Original Message ----- From: "Bell, Pat" To: Sent: Wednesday, June 17, 2009 12:35 PM Subject: [Histonet] RNA In Situ Hybridization I want to learn to do In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have the Dako Autostainer, but I know that some of the procedure would be done manually. I would appreciate any protocols that would help me. Thank you for your help. Pat Pat Bell HT(ASCP) Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.76/2183 - Release Date: 06/17/09 05:53:00 -------------- next part -------------- No virus found in this outgoing message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.76/2183 - Release Date: 06/17/09 05:53:00 From susie.gawley <@t> gmail.com Wed Jun 17 14:21:42 2009 From: susie.gawley <@t> gmail.com (Susie Gawley) Date: Wed Jun 17 14:21:46 2009 Subject: [Histonet] histolgy jobs Message-ID: <323cf0f50906171221o9fdbb5crbcf4603c0a57df28@mail.gmail.com> I am looking for histology job, preferably a day shift !! I am registered ASCP ! I live in Dallas & have a job at this time ! Do you know of any jobs at UTSW ?? I would surely appreciate it, if you would let me know. Thank you From d-emge <@t> northwestern.edu Wed Jun 17 16:07:14 2009 From: d-emge <@t> northwestern.edu (d-emge@northwestern.edu) Date: Wed Jun 17 16:07:18 2009 Subject: [Histonet] Endogenous GFP frozen sectioning Message-ID: <20090617210714.E0682CF@lulu.it.northwestern.edu> I have been asked to section 10 dpn mouse ovaries embedded in OCT for endogenous GFP. These are very, very tiny. The investigator would lik asked for at 5 microns an best for this? Thanks, Donna Donna J. Emge, Research Histology and Phen Northwestern University 710 N. Fairbanks 312-503-2679 [1]d-emge@northwestern.edu References 1. 3D"mailto:d-emge@north From a.babri <@t> uq.edu.au Wed Jun 17 18:14:25 2009 From: a.babri <@t> uq.edu.au (Awais Babri) Date: Wed Jun 17 18:08:01 2009 Subject: [Histonet] Re: Histonet Digest, Vol 67, Issue 17 In-Reply-To: <200906171712.n5HHC9Hf002975@mailhub4.uq.edu.au> Message-ID: From: Reply-To: Date: Thu, 18 Jun 2009 03:12:15 +1000 To: Conversation: Histonet Digest, Vol 67, Issue 17 Subject: Histonet Digest, Vol 67, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. PR and ER staining in dog uterus (Karen Doty) 2. Re: Inactivation of DAB (Sherwood, Margaret ) 3. RE: Research Genetics (Reynolds,Donna M) 4. Looking for NYU contact (Angela Bitting) 5. Re: Re: Inactivation of DAB (Lynette Pavelich) 6. RE: Re: Inactivation of DAB (CHRISTIE GOWAN) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Jun 2009 15:10:06 -0500 From: Karen Doty Subject: [Histonet] PR and ER staining in dog uterus To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, Folks, We are starting to work on a project that requires PR and ER staining in dog uterus. I'm hoping someone will share some tips such as which antibodies work well in dog (clone, manufacturer), and any specific special procedures that might help, such as which antigen retrieval methods work best. I've tried a couple antibodies already without good results in dog although they worked very well in a mouse control. All suggestions and advice is appreciated. Thanks very much. Karen Doty Dept. of Veterinary Biosciences University of Illinois _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009 ------------------------------ Message: 2 Date: Wed, 17 Jun 2009 09:30:55 -0400 From: "Sherwood, Margaret " Subject: [Histonet] Re: Inactivation of DAB To: "histonet" Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 3 Date: Wed, 17 Jun 2009 08:33:15 -0500 From: "Reynolds,Donna M" Subject: [Histonet] RE: Research Genetics To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <785BBF0C5F49CE41BA74460A43A08F0214492216E7@DCPWVMBXC0VS3.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Research Genetics has merged a couple of time the last few years. They are now Open Biosystems in Huntsville, Al pho 888 412 2225. They do not list the Research Genetics on the Web but last I knew products were still available. You can call them to get current catalog number and prices. Donna Reynolds HT (ASCP) Chief Histology Laboratory U. T. M.D. Cancer Center Cancer Biology Core Immunohisto Lab 713-792-8106 Message: 2 Date: Mon, 15 Jun 2009 14:52:39 -0400 From: "Joanne Mauger" Subject: [Histonet] Re: research genetics/phoenix biotech To: , Message-ID: <4A3660370200003100007C32@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Hi everyone, I am unable to find this company anymore. Does anyone know if old research genetics products are still being sold? I don't want Invitrogen- they did away with the product I want-MicroHyb. Thanks, Jo Mauger ------------------------------ **** ------------------------------ Message: 4 Date: Wed, 17 Jun 2009 09:50:09 -0400 From: "Angela Bitting" Subject: [Histonet] Looking for NYU contact To: Message-ID: <4A38BC51.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Just wondering if anyone can give me contact information for someone in the Neuropath Lab at NYU? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 5 Date: Wed, 17 Jun 2009 10:05:13 -0400 From: "Lynette Pavelich" Subject: Re: [Histonet] Re: Inactivation of DAB To: , Message-ID: <4A38BFD9020000EE0002A178@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184. 1. Prepare the following aqeous stock solutions: a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter) b. 2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter) 2. Dilute DAB solution of necessary so that its concentration does not exceed 0.9 mg/ml. 3. For each 10ml of DAB solution, add: a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter) b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%) 4. Allow mixture to stand for at least 10 hours. It is now non-mutagenic. 5. Decolorize the mixture with ascorbic acid (add powder until color disappears). This too is an oxidation/reduction reaction. 6. Neutralize the decolorized mixture with sodium bicarbonate (test with pH meter, pH paper or dipsticks). Note: we recommend using magnesium hydroxide/oxide instead of sodium bicarbonate. 7. Discard the solution down the drain provided that local wastewater authorities have given their approval. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Sherwood, Margaret " 06/17/09 9:30 AM >>> We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 17 Jun 2009 14:27:39 +0000 From: CHRISTIE GOWAN Subject: RE: [Histonet] Re: Inactivation of DAB To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Peggy, You wrote: > We have started doing routine immunohistochemistry on our stainer and have a > large waste container that includes diaminobenzidine (DAB). > > We would like to inactivate it so we don't have to pay to have the waste > removed. Can anyone help me? What do people use to inactivate their DAB? May I suggest that you access the histonet archives where you will be able to see all the previous discussions on this topic. The responses were many and varied depending on different lab types and locations. Good luck with your search. Respectfully, Christie Gowan Surgical Pathology University of Alabama Hospital ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 17 **************************************** No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.71/2178 - Release Date: 06/16/09 21:23:00 From sshawdfy <@t> med.kobe-u.ac.jp Wed Jun 17 18:41:16 2009 From: sshawdfy <@t> med.kobe-u.ac.jp (shymaa shawadfy) Date: Wed Jun 17 18:41:20 2009 Subject: [Histonet] Rat anti-L1 Chemicon Message-ID: <95680B8631414EF79AE75383241AA57B@8A287A4ADEF0487> Dear all I need some advice concerning immunostaining with Anti-Neural Cell Adhesion Molecule L1 from chemicon on P0 mice brains. I am trying to use this antibody for formalin fixed paraffin embedded sections but I can not get any signal until now, I tried antigen retrieval with 10 mM citrate buffer and I tried different antibody concentrations. I will appreciate any comment Thanks a lot Shymaa From eyee <@t> dpmginc.com Wed Jun 17 19:53:27 2009 From: eyee <@t> dpmginc.com (Ellen Yee) Date: Wed Jun 17 19:49:55 2009 Subject: [Histonet] TTF-1 Message-ID: <20090618005327.af5eea50@mail.dpmginc.com> Hi everyone, Has anyone had problems with TTF-1 not staining Bouin's fixed tissue. We stain ours on the DAKO Autostainer with Envision+ Dual Link HRP System. We use DAKO pH9 target retrieval in the pressure cooker, and use a 1:50 dilution of DAKO TTF-1 (M3575). Ellen Yee Diagnostic Pathology Medical Group, Inc. Sacramento, CA From kmerriam2003 <@t> yahoo.com Thu Jun 18 06:42:49 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jun 18 06:42:54 2009 Subject: [Histonet] aggrecan IHC on articular cartilage Message-ID: <961674.22336.qm@web50310.mail.re2.yahoo.com> Hi Everyone, I was wondering if anyone has done aggrecan IHC?on articular cartilage from FFPE/decaled rodent?knees.??The references and antibodies all call for a funky pretreatment step rather than a routine heat?or enzyme (granted all of?the references I have found are all for westerns?and not IHC).? Anyway, the pretreatment calls for incubation with chondroitinase or a combination of chondriotinase/keratinase 1 and 2 (it seems as though the aggrecan is bound up in these other proteins and they need to be digested away to access the aggrecan). Has anyone does this stain on tissues using a more routine retrieval?? Also, any antibodies to recommend?? ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From PVerden <@t> UROPARTNERS.COM Thu Jun 18 08:55:49 2009 From: PVerden <@t> UROPARTNERS.COM (Paul Verden) Date: Thu Jun 18 08:55:53 2009 Subject: [Histonet] used equipment Message-ID: Greetings. We are in the market for a used gravity oven, probably about 2-3 cu. ft. in capacity. We would like to know what equipment remanufacturers you have used and what ones you liked. Thanks. Paul UroPartners, LLC From BBabinsack <@t> genesishcs.org Mon Jun 15 10:54:04 2009 From: BBabinsack <@t> genesishcs.org (Benny Babinsack) Date: Thu Jun 18 12:20:37 2009 Subject: [Histonet] Synaptophysin Message-ID: We use a IVD from Cellmarque and use pa ncreas for the control Benny Ba Lab Services Senior Tech,&n (740)454-5587 ***Confidentiality Notice*** The information contained in this e-mail message ([Histonet] Synaptophysin), including any attachments, are intended only for use of the individual or entity named above (histonet@lists.utsouthwestern.edu). This e-mail may contain information that is privileged, confidential and/or otherwise exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, any disclosure, dissemination, distribution, copying or other use of the communication or its substance is prohibited. If you have received this e-mail in error, please reply to this e-mail indicating you are not the intended recipient and immediately destroy all copies of this e-mail. Receipt by anyone other than the intended recipient is not a waiver of any privileged information. From ROrr <@t> northshore.org Tue Jun 16 12:29:47 2009 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Thu Jun 18 12:20:39 2009 Subject: [Histonet] biocare bcl 6 In-Reply-To: Message-ID: Message: 3 Date: Mon, 15 Jun 2009 15:34:33 -0400 From: "Justin Peters" Subject: [Histonet] BCL-6 To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F05DFAF54@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" I am having trouble getting bcl-6 antibody to work (Biocare CM223). Does anyone have a good protocol for me to try? Hi Justin, I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent. Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED". I also use Mach4 detection... if problems persist, contact me offline, I may be able to help with some other suggestions. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********************* From Popp.Laurie <@t> mayo.edu Tue Jun 16 15:59:46 2009 From: Popp.Laurie <@t> mayo.edu (Popp, Laurie A.) Date: Thu Jun 18 12:20:40 2009 Subject: [Histonet] Cytokeratin 18 M30 apoptosis staining Message-ID: <1488342ADB74EC4E969C48BDCDE0BD98FB7735@msgebe23.mfad.mfroot.org> Has anyone worked with this particular antibody? Can you give me any advise as far as what worked? I have an investigator that wants to use this antibody in diseased liver tissue and nothing that I am trying is working. Thanks! Laurie Laurie Popp, BA HT ASCP cm TACMA Shared Services Mayo Clinic-Rochester, MN From Pat.Bell <@t> ucdenver.edu Tue Jun 16 16:05:41 2009 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Thu Jun 18 12:20:42 2009 Subject: [Histonet] RNA In Situ Hybridizaton Message-ID: <64DB27005E2FD3439E88502D7A5C9121877CB00392@CORTEZ.ucdenver.pvt> My supervisor wants me to learn In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have a Dako Autostainer, but I know that some of the procedure would be manual. I would appreciate any protocols that would help me. Thank you for your help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From Popp.Laurie <@t> mayo.edu Wed Jun 17 12:08:08 2009 From: Popp.Laurie <@t> mayo.edu (Popp, Laurie A.) Date: Thu Jun 18 12:20:43 2009 Subject: [Histonet] FW: Cytokeratin 18 M30 apoptosis staining Message-ID: <1488342ADB74EC4E969C48BDCDE0BD98FB7739@msgebe23.mfad.mfroot.org> > Has anyone worked with this particular antibody? Can you give me any > advise as far as what worked? I have an investigator that wants to > use this antibody in diseased liver tissue and nothing that I am > trying is working. > > Thanks! > Laurie > > Laurie Popp, BA HT ASCP cm > TACMA Shared Services > Mayo Clinic-Rochester, MN > > From srwilkes <@t> gmail.com Thu Jun 18 14:38:22 2009 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Thu Jun 18 14:38:28 2009 Subject: [Histonet] Glypican-3 on BondMax Message-ID: <8e5827cf0906181238x6ee4b038hc98ea547ee2939a@mail.gmail.com> Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwilkes@gmail.com From srwilkes <@t> gmail.com Thu Jun 18 14:42:12 2009 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Thu Jun 18 14:42:17 2009 Subject: [Histonet] Glypican-3 on BondMax Message-ID: <8e5827cf0906181242nf88a092o4a3868a7b4ba3dd7@mail.gmail.com> Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwilkes@gmail.com From srwilkes <@t> hotmail.com Thu Jun 18 14:39:27 2009 From: srwilkes <@t> hotmail.com (Steven Wilkes) Date: Thu Jun 18 16:05:10 2009 Subject: [Histonet] Glypican-3 on BondMax Message-ID: Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwilkes@gmail.com _________________________________________________________________ Bing? brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1 From lynnd01 <@t> hotmail.com Thu Jun 18 19:47:14 2009 From: lynnd01 <@t> hotmail.com (Lynn Dike) Date: Thu Jun 18 19:47:19 2009 Subject: [Histonet] xylene and frozen sections Message-ID: Lynn _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From lldewe <@t> gmail.com Thu Jun 18 20:30:09 2009 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Thu Jun 18 20:30:13 2009 Subject: [Histonet] Temp work Message-ID: <7173d3c00906181830o27dfc5d3sbd8cc8d5050066e5@mail.gmail.com> Hi everyone, I am looking for temp work. If you have availability on a travel basis let me know. I have 10+ years experience with histology and IHC in a research venue and am published! Cheers, Loralei From jessgrocki <@t> yahoo.com Fri Jun 19 07:51:58 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Fri Jun 19 07:52:01 2009 Subject: [Histonet] Diff Quik II Message-ID: <150374.59014.qm@web82008.mail.mud.yahoo.com> Good Morning! ? I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From derek.papalegis <@t> tufts.edu Fri Jun 19 08:11:05 2009 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Fri Jun 19 08:11:08 2009 Subject: [Histonet] reticulin stain Message-ID: <4A3B8E69.3090403@tufts.edu> I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From rjbuesa <@t> yahoo.com Fri Jun 19 08:26:34 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 19 08:26:37 2009 Subject: [Histonet] reticulin stain Message-ID: <431812.3131.qm@web65714.mail.ac4.yahoo.com> Derek: I am sending it to you under separate cover. Ren? J. --- On Fri, 6/19/09, Derek Papalegis wrote: From: Derek Papalegis Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 9:11 AM I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrobertson <@t> pathologysciences.com Fri Jun 19 08:49:52 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Fri Jun 19 08:49:55 2009 Subject: [Histonet] Diff Quik II In-Reply-To: <150374.59014.qm@web82008.mail.mud.yahoo.com> References: <150374.59014.qm@web82008.mail.mud.yahoo.com> Message-ID: <518CD6920AA7154193CBE5977CD880733A8A7C@psmgsrv2.PSMG.local> I purchase Diff Quik Solution II in a 2.5L bottle from Cardinal Health Catalog # B4132-12A. It's not something that they have in stock and it usually ships from Dade Behring Inc. which is who makes it. It usually takes me a couple of weeks to get it, but I have no problem receiving it. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, June 19, 2009 5:52 AM To: histonet Subject: [Histonet] Diff Quik II Good Morning! ? I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dreynold <@t> mdanderson.org Fri Jun 19 09:28:09 2009 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Fri Jun 19 09:28:28 2009 Subject: [Histonet] Re: anti Brdu In-Reply-To: References: Message-ID: <785BBF0C5F49CE41BA74460A43A08F0214492216F0@DCPWVMBXC0VS3.mdanderson.edu> We have used mouse anti-Brdu from Becton Dickinson for years. It is still available from BD BioSciences Cat. 347580. I know it is made in mouse but we used an IgG1 secondary and always had good results. There may be some cytoplasmic label, probably background, but you are interested in the nuclear staining and it is very distinct. We have used it on both frozen and paraffin processed tissues. Donna Reynolds, HT (ASCP) Dept. Cancer Biology U.T. M.D. Anderson Cancer Center, Houston Tx Message: 3 Date: Wed, 17 Jun 2009 14:26:09 -0400 From: "Madary, Joseph" Subject: [Histonet] source for rat anti mouse brdu? To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have not done a brdu on mouse in five years. Used Harlan but they are not selling that anymore. I used a procedure using rat anti mouse brdu primary, rabbiot anti rat mouse adsorbed secondary, then ABC/DAB. Anyone have any idea on a good source? I used biocompare and found stuff but would like to hear comments. Also anyone use conjugated brdu? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) ***************** From tfountain <@t> exchange.hsc.mb.ca Fri Jun 19 10:42:09 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Fri Jun 19 10:42:15 2009 Subject: [Histonet] Ventana Reagent Cost?? Message-ID: Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS: CC1: CC2: EZ Prep: Reaction Buffer: I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From dsv22 <@t> hotmail.com Fri Jun 19 10:52:15 2009 From: dsv22 <@t> hotmail.com (Denise Van Eaton) Date: Fri Jun 19 10:52:19 2009 Subject: [Histonet] Good Used Equipment for Sale Message-ID: Good Morning HistoNetters- Good Used equipment for sale! We have a Leica TP1050 bench model that is currently being used on a daily basis. The 300 cassette capacity retort, will process with or without heat, with or without pressure or vacuum. It has the reagent management system to assist with ?rotation? of reagents as well as the xylene extraction cycle for the paraffin. We purchased it ?used? about 2 years ago and it has worked very well for us. We just need the space more than the additional processor. We also have a 4+ year old Dako Autostainer. Each run will hold 44 slides. It is an open system, and so will allow you to use whatever reagents you choose. This unit has worked flawlessly for us, but we are discontinuing our on-site IHC program. Both of these units have been well maintained, are sold ?as is? and will need to be picked up by the purchaser. We have no shipping crates and/or the muscles to pack up either machine. We are located just east of the Kansas City, Missouri area in Blue Springs. Anyone interested, please contact Denise Van Eaton @ dvaneaton@littonlab.com _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009 From thachdc <@t> niaid.nih.gov Fri Jun 19 10:56:27 2009 From: thachdc <@t> niaid.nih.gov (Thach, Dzung (NIH/NIAID) [E]) Date: Fri Jun 19 10:56:32 2009 Subject: [Histonet] Mouse necropsy question In-Reply-To: Message-ID: Hi Everyone!! I am collecting spleen, brain, and spinal cord from virus infected mouse. I will perfuse mouse with PBS. Do you think I need to use a new pair of scissors and forceps for each tissue in each mouse and between each mouse? Thanks, Dz From rjbuesa <@t> yahoo.com Fri Jun 19 10:57:20 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 19 10:57:24 2009 Subject: [Histonet] Ventana Reagent Cost?? Message-ID: <737537.89352.qm@web65709.mail.ac4.yahoo.com> `If you don't use their auto stainer probably you will not be able to use their antibodies that, as far as I know, are "optimized" to be used in their instrument in what they call "liquid cover slips" for room and high temperature. Ren? J. --- On Fri, 6/19/09, Tiana Fountain wrote: From: Tiana Fountain Subject: [Histonet] Ventana Reagent Cost?? To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 11:42 AM Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS: CC1: CC2: EZ Prep: Reaction? Buffer: I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana -----Inline Attachment Follows----- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information.? Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.? If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Fri Jun 19 11:46:37 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Jun 19 11:47:10 2009 Subject: [Histonet] Ventana Reagent Cost?? References: Message-ID: The prices I have are on the list you sent. I couldn't find the CC2 price quickly. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tiana Fountain Sent: Fri 6/19/2009 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Reagent Cost?? Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS:$98.40 CC1:$565.80 CC2: EZ Prep:$37.80 Reaction Buffer:$43.80 I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana From dunatrsd <@t> sbcglobal.net Fri Jun 19 12:08:47 2009 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Fri Jun 19 12:08:51 2009 Subject: [Histonet] Ventana Reagent Cost?? In-Reply-To: References: Message-ID: <707612.54476.qm@web83908.mail.sp1.yahoo.com> Ventana prices will vary dependednt upon?your (not you per se, but rather your instrument)?consumption. Here is what we pay: LCS??? $74 CC1??? $832 CC2??? $895 EZprep (10X)?$452 Reaction Buffer$65 If you need more info, please contact me Thanks Dusko Trajkovic Pfizer Inc La Jolla ________________________________ From: "Burton, Lynn" To: Tiana Fountain ; histonet@lists.utsouthwestern.edu Sent: Friday, June 19, 2009 9:46:37 AM Subject: RE: [Histonet] Ventana Reagent Cost?? The prices I have are on the list you sent. I couldn't find the CC2 price quickly. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tiana Fountain Sent: Fri 6/19/2009 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Reagent Cost?? Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS:$98.40 CC1:$565.80 CC2: EZ Prep:$37.80 Reaction? Buffer:$43.80 I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbisher <@t> Princeton.EDU Fri Jun 19 12:14:14 2009 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Fri Jun 19 12:14:21 2009 Subject: [Histonet] Microtome - Free! Message-ID: I have a pretty old microtome to donate. It is a Leitz 1512. I also have some of those big blades that it uses. All you have to do is pay for the shipping. Cheers, Maggie Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu From MLunetta <@t> luhcares.org Fri Jun 19 12:20:55 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Fri Jun 19 12:21:07 2009 Subject: [Histonet] Re: retic stain Message-ID: <4A3B7497020000A8000326F2@mail.luhcares.org> I too will send you ours to your e-mail. Matt Lunetta HT (ASCP) Message: 13 Date: Fri, 19 Jun 2009 06:26:34 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu, Derek Papalegis Message-ID: <431812.3131.qm@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Derek: I am sending it to you under separate cover. Ren? J. --- On Fri, 6/19/09, Derek Papalegis wrote: From: Derek Papalegis Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 9:11 AM I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From vapatpxs <@t> yahoo.com Fri Jun 19 12:35:03 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Fri Jun 19 12:35:11 2009 Subject: [Histonet] LR White and immunofluorescence Message-ID: <778498.55603.qm@web46113.mail.sp1.yahoo.com> Happy Friday Everybody, I have a user who has a precious sample and wants to embed in LR White, maybe. I'm having him practice on something not precious first. My questions are: 1. He wants to do immunofluorescence and morphology on these nerve samples. He is planning on perfusing in 2% PFA, 0.1% glut in 0.1M sodium cacodylate. Then he will dissect out the nerve, cut it in half and place half in 2% PFA, 0.1% glut (embed this in LR White) and the other half in 4% PFA, 2.5% glut (embed this in Epon) to finish the fixation. Will that work? Or will the perfusion fixation with the weaker fix cause the stronger submersion fix to be moot? 2. He wants to do immunofluorescence on the LR white sample. I figure, primary antibody, then secondary with glow is no different that secondary with gold ball, am I correct in this? Thanks and have a great weekend. All aglow waiting for your answer, Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. From rsrichmond <@t> gmail.com Fri Jun 19 13:05:18 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jun 19 13:05:24 2009 Subject: [Histonet] Re: Diff-Quik II Message-ID: To stain Helicobacter, you need only the Diff-Quik II solution. This is a brand name. I've used several generic equivalents and they work also. Bob Richmond Samurai Pathologist Knoxville TN From diego.rodriguezgil <@t> yale.edu Fri Jun 19 13:23:36 2009 From: diego.rodriguezgil <@t> yale.edu (Diego) Date: Fri Jun 19 13:23:41 2009 Subject: [Histonet] Electron Microscopy In-Reply-To: <200903161643.n2GGhO5l008751@mr3.its.yale.edu> References: <200903161643.n2GGhO5l008751@mr3.its.yale.edu> Message-ID: <4A3BD7A8.30209@yale.edu> Hi, I am trying to get a a pre-embedding immuno to work. I have tried several different things (fixatives, incubation times, +/- detergent) and it seems that definitely I need to have a detergent present. I used 0.05% Tween20, but the quality of the EM is really bad (it is impossible to distinguish anything from them). Does anybody have experience doing this? Any protocol I could try? Suggestions? Thanks very much in advance for any help. Diego. -- Diego J. Rodriguez Gil Postdoctoral Associate Department of Neurosurgery Yale University School of Medicine PO Box 208082 333 Cedar St, FMB-430 New Haven CT, 06520-8082 U.S.A. Phone: 203-785-4230 From Herrick.James <@t> mayo.edu Fri Jun 19 14:16:11 2009 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Fri Jun 19 14:16:15 2009 Subject: [Histonet] Section Mounting Techniques Message-ID: <7267A64D75F58241B577876D8A885631191F59@msgebe41> Hi everyone, Does anyone have a good technique for mounting bone tissue (rabbit calvaria embedded in plastic) to coated slides? I am having problems with wrinkles following the rolling of the sections onto the slides. I would greatly appreciate any help you might be willing to offer. Thanks. Jim From thisisann <@t> aol.com Fri Jun 19 14:25:19 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Jun 19 14:25:44 2009 Subject: [Histonet] Peloris Tissue Processor Message-ID: <8CBBF2AD175ACC6-E78-534@WEBMAIL-DY33.sysops.aol.com> Does anyone use Formula 83 as their Clearant on a Peloris tissue processor?? If so, have you had any problems?? Please let me know as we are experiencing consistent problems with water in the Formula 83. Thank you, Ann From jkeller <@t> schospital.com Fri Jun 19 15:05:29 2009 From: jkeller <@t> schospital.com (Judy Keller) Date: Fri Jun 19 15:05:45 2009 Subject: [Histonet] RE: Diff Quik II References: <20090619170126.DC8EF20C9FD4@barracuda.schospital.com> Message-ID: <4EC9FD7ABF9B0C4B92D8CCA2E2EC932D074D6C@star.schhs.org> We buy Diff Quik II by the gallon directly from Dade. You don't need the kit. Judy Keller, HTL South County Hospital Wakefield, RI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Fri 6/19/2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 67, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Synaptophysin (Benny Babinsack) 2. biocare bcl 6 (Orr, Rebecca) 3. Cytokeratin 18 M30 apoptosis staining (Popp, Laurie A.) 4. RNA In Situ Hybridizaton (Bell, Pat) 5. FW: Cytokeratin 18 M30 apoptosis staining (Popp, Laurie A.) 6. Glypican-3 on BondMax (Steven Wilkes) 7. Glypican-3 on BondMax (Steven Wilkes) 8. Glypican-3 on BondMax (Steven Wilkes) 9. xylene and frozen sections (Lynn Dike) 10. Temp work (Loralei Dewe) 11. Diff Quik II (Jessica Piche) 12. reticulin stain (Derek Papalegis) 13. reticulin stain (Rene J Buesa) 14. RE: Diff Quik II (Jodie Robertson) 15. Re: anti Brdu (Reynolds,Donna M) 16. Ventana Reagent Cost?? (Tiana Fountain) 17. Good Used Equipment for Sale (Denise Van Eaton) 18. Mouse necropsy question (Thach, Dzung (NIH/NIAID) [E]) 19. Re: Ventana Reagent Cost?? (Rene J Buesa) 20. RE: Ventana Reagent Cost?? (Burton, Lynn) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Jun 2009 11:54:04 -0400 From: Benny Babinsack Subject: [Histonet] Synaptophysin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" We use a IVD from Cellmarque and use pa ncreas for the control Benny Ba Lab Services Senior Tech,&n (740)454-5587 ***Confidentiality Notice*** The information contained in this e-mail message ([Histonet] Synaptophysin), including any attachments, are intended only for use of the individual or entity named above (histonet@lists.utsouthwestern.edu). This e-mail may contain information that is privileged, confidential and/or otherwise exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, any disclosure, dissemination, distribution, copying or other use of the communication or its substance is prohibited. If you have received this e-mail in error, please reply to this e-mail indicating you are not the intended recipient and immediately destroy all copies of this e-mail. Receipt by anyone other than the intended recipient is not a waiver of any privileged information. ------------------------------ Message: 2 Date: Tue, 16 Jun 2009 12:29:47 -0500 From: "Orr, Rebecca" Subject: [Histonet] biocare bcl 6 To: "'histonet@lists.utsouthwestern.edu'" Cc: "'JPeters@bostwicklaboratories.com'" Message-ID: Content-Type: text/plain; charset="us-ascii" Message: 3 Date: Mon, 15 Jun 2009 15:34:33 -0400 From: "Justin Peters" Subject: [Histonet] BCL-6 To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F05DFAF54@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" I am having trouble getting bcl-6 antibody to work (Biocare CM223). Does anyone have a good protocol for me to try? Hi Justin, I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent. Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED". I also use Mach4 detection... if problems persist, contact me offline, I may be able to help with some other suggestions. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********************* ------------------------------ Message: 3 Date: Tue, 16 Jun 2009 15:59:46 -0500 From: "Popp, Laurie A." Subject: [Histonet] Cytokeratin 18 M30 apoptosis staining To: Message-ID: <1488342ADB74EC4E969C48BDCDE0BD98FB7735@msgebe23.mfad.mfroot.org> Content-Type: text/plain; charset="US-ASCII" Has anyone worked with this particular antibody? Can you give me any advise as far as what worked? I have an investigator that wants to use this antibody in diseased liver tissue and nothing that I am trying is working. Thanks! Laurie Laurie Popp, BA HT ASCP cm TACMA Shared Services Mayo Clinic-Rochester, MN ------------------------------ Message: 4 Date: Tue, 16 Jun 2009 15:05:41 -0600 From: "Bell, Pat" Subject: [Histonet] RNA In Situ Hybridizaton To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <64DB27005E2FD3439E88502D7A5C9121877CB00392@CORTEZ.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" My supervisor wants me to learn In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have a Dako Autostainer, but I know that some of the procedure would be manual. I would appreciate any protocols that would help me. Thank you for your help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu ------------------------------ Message: 5 Date: Wed, 17 Jun 2009 12:08:08 -0500 From: "Popp, Laurie A." Subject: [Histonet] FW: Cytokeratin 18 M30 apoptosis staining To: Message-ID: <1488342ADB74EC4E969C48BDCDE0BD98FB7739@msgebe23.mfad.mfroot.org> Content-Type: text/plain; charset="US-ASCII" > Has anyone worked with this particular antibody? Can you give me any > advise as far as what worked? I have an investigator that wants to > use this antibody in diseased liver tissue and nothing that I am > trying is working. > > Thanks! > Laurie > > Laurie Popp, BA HT ASCP cm > TACMA Shared Services > Mayo Clinic-Rochester, MN > > ------------------------------ Message: 6 Date: Thu, 18 Jun 2009 15:38:22 -0400 From: Steven Wilkes Subject: [Histonet] Glypican-3 on BondMax To: histonet@lists.utsouthwestern.edu Message-ID: <8e5827cf0906181238x6ee4b038hc98ea547ee2939a@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwilkes@gmail.com ------------------------------ Message: 7 Date: Thu, 18 Jun 2009 15:42:12 -0400 From: Steven Wilkes Subject: [Histonet] Glypican-3 on BondMax To: histonet@lists.utsouthwestern.edu Message-ID: <8e5827cf0906181242nf88a092o4a3868a7b4ba3dd7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwilkes@gmail.com ------------------------------ Message: 8 Date: Thu, 18 Jun 2009 15:39:27 -0400 From: Steven Wilkes Subject: [Histonet] Glypican-3 on BondMax To: histology jobs Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwilkes@gmail.com _________________________________________________________________ Bing(tm) brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1 ------------------------------ Message: 9 Date: Thu, 18 Jun 2009 19:47:14 -0500 From: Lynn Dike Subject: [Histonet] xylene and frozen sections To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Lynn _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 ------------------------------ Message: 10 Date: Thu, 18 Jun 2009 18:30:09 -0700 From: Loralei Dewe Subject: [Histonet] Temp work To: histonet@lists.utsouthwestern.edu Message-ID: <7173d3c00906181830o27dfc5d3sbd8cc8d5050066e5@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi everyone, I am looking for temp work. If you have availability on a travel basis let me know. I have 10+ years experience with histology and IHC in a research venue and am published! Cheers, Loralei ------------------------------ Message: 11 Date: Fri, 19 Jun 2009 05:51:58 -0700 (PDT) From: Jessica Piche Subject: [Histonet] Diff Quik II To: histonet Message-ID: <150374.59014.qm@web82008.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good Morning! I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ------------------------------ Message: 12 Date: Fri, 19 Jun 2009 09:11:05 -0400 From: Derek Papalegis Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Message-ID: <4A3B8E69.3090403@tufts.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 ------------------------------ Message: 13 Date: Fri, 19 Jun 2009 06:26:34 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu, Derek Papalegis Message-ID: <431812.3131.qm@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Derek: I am sending it to you under separate cover. Ren? J. --- On Fri, 6/19/09, Derek Papalegis wrote: From: Derek Papalegis Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 9:11 AM I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 19 Jun 2009 06:49:52 -0700 From: "Jodie Robertson" Subject: RE: [Histonet] Diff Quik II To: "Jessica Piche" , "histonet" Message-ID: <518CD6920AA7154193CBE5977CD880733A8A7C@psmgsrv2.PSMG.local> Content-Type: text/plain; charset="iso-8859-1" I purchase Diff Quik Solution II in a 2.5L bottle from Cardinal Health Catalog # B4132-12A. It's not something that they have in stock and it usually ships from Dade Behring Inc. which is who makes it. It usually takes me a couple of weeks to get it, but I have no problem receiving it. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, June 19, 2009 5:52 AM To: histonet Subject: [Histonet] Diff Quik II Good Morning! I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 19 Jun 2009 09:28:09 -0500 From: "Reynolds,Donna M" Subject: [Histonet] Re: anti Brdu To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <785BBF0C5F49CE41BA74460A43A08F0214492216F0@DCPWVMBXC0VS3.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" We have used mouse anti-Brdu from Becton Dickinson for years. It is still available from BD BioSciences Cat. 347580. I know it is made in mouse but we used an IgG1 secondary and always had good results. There may be some cytoplasmic label, probably background, but you are interested in the nuclear staining and it is very distinct. We have used it on both frozen and paraffin processed tissues. Donna Reynolds, HT (ASCP) Dept. Cancer Biology U.T. M.D. Anderson Cancer Center, Houston Tx Message: 3 Date: Wed, 17 Jun 2009 14:26:09 -0400 From: "Madary, Joseph" Subject: [Histonet] source for rat anti mouse brdu? To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have not done a brdu on mouse in five years. Used Harlan but they are not selling that anymore. I used a procedure using rat anti mouse brdu primary, rabbiot anti rat mouse adsorbed secondary, then ABC/DAB. Anyone have any idea on a good source? I used biocompare and found stuff but would like to hear comments. Also anyone use conjugated brdu? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) ***************** ------------------------------ Message: 16 Date: Fri, 19 Jun 2009 10:42:09 -0500 From: "Tiana Fountain" Subject: [Histonet] Ventana Reagent Cost?? To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS: CC1: CC2: EZ Prep: Reaction Buffer: I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 17 Date: Fri, 19 Jun 2009 10:52:15 -0500 From: Denise Van Eaton Subject: [Histonet] Good Used Equipment for Sale To: histonetmailinglist Message-ID: Content-Type: text/plain; charset="Windows-1252" Good Morning HistoNetters- Good Used equipment for sale! We have a Leica TP1050 bench model that is currently being used on a daily basis. The 300 cassette capacity retort, will process with or without heat, with or without pressure or vacuum. It has the reagent management system to assist with "rotation" of reagents as well as the xylene extraction cycle for the paraffin. We purchased it "used" about 2 years ago and it has worked very well for us. We just need the space more than the additional processor. We also have a 4+ year old Dako Autostainer. Each run will hold 44 slides. It is an open system, and so will allow you to use whatever reagents you choose. This unit has worked flawlessly for us, but we are discontinuing our on-site IHC program. Both of these units have been well maintained, are sold "as is" and will need to be picked up by the purchaser. We have no shipping crates and/or the muscles to pack up either machine. We are located just east of the Kansas City, Missouri area in Blue Springs. Anyone interested, please contact Denise Van Eaton @ dvaneaton@littonlab.com _________________________________________________________________ Hotmail? has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009 ------------------------------ Message: 18 Date: Fri, 19 Jun 2009 11:56:27 -0400 From: "Thach, Dzung (NIH/NIAID) [E]" Subject: [Histonet] Mouse necropsy question To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Everyone!! I am collecting spleen, brain, and spinal cord from virus infected mouse. I will perfuse mouse with PBS. Do you think I need to use a new pair of scissors and forceps for each tissue in each mouse and between each mouse? Thanks, Dz ------------------------------ Message: 19 Date: Fri, 19 Jun 2009 08:57:20 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Ventana Reagent Cost?? To: histonet@lists.utsouthwestern.edu, Tiana Fountain Message-ID: <737537.89352.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 `If you don't use their auto stainer probably you will not be able to use their antibodies that, as far as I know, are "optimized" to be used in their instrument in what they call "liquid cover slips" for room and high temperature. Ren? J. --- On Fri, 6/19/09, Tiana Fountain wrote: From: Tiana Fountain Subject: [Histonet] Ventana Reagent Cost?? To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 11:42 AM Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS: CC1: CC2: EZ Prep: Reaction Buffer: I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana -----Inline Attachment Follows----- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 19 Jun 2009 11:46:37 -0500 From: "Burton, Lynn" Subject: RE: [Histonet] Ventana Reagent Cost?? To: "Tiana Fountain" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" The prices I have are on the list you sent. I couldn't find the CC2 price quickly. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tiana Fountain Sent: Fri 6/19/2009 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Reagent Cost?? Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS:$98.40 CC1:$565.80 CC2: EZ Prep:$37.80 Reaction Buffer:$43.80 I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 19 **************************************** From kmilne <@t> bccancer.bc.ca Fri Jun 19 15:26:37 2009 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Fri Jun 19 15:26:57 2009 Subject: [Histonet] Superfrost plus gold slides vs superfrost plus In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC0657807BBEE2A@srvex03.phsabc.ehcnet.ca> Hi everyone, I'm curious if anyone has tried using Superfrost plus gold slides, are they any better than the regular superfrost plus? I'm dealing with frozen lymph node on a Ventana discovery, I've tried a few different fixing/drying combinations but I still have tissue lifting (not completely but enough to be bothersome!). Wondering if it's worth giving the gold slides a go. Thanks, Katy From mbisher <@t> Princeton.EDU Fri Jun 19 15:34:58 2009 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Fri Jun 19 15:35:08 2009 Subject: [Histonet] Superfrost plus gold slides vs superfrost plus In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC0657807BBEE2A@srvex03.phsabc.ehcnet.ca> Message-ID: I had a group here who were having trouble with their cryo-sections sticking to the slides. Their samples were rat brains. He found that the Gold Super Frost Plus worked well with in-situ but not for immuno. At least that is the comment he wrote down and put on our bulletin board in the lab. Most of the people in the lab tend to use the Super Frost Plus (not the Gold) and they seem pretty happy with them. Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 6/19/09 4:26 PM, "Milne, Katy" wrote: > Hi everyone, > > I'm curious if anyone has tried using Superfrost plus gold slides, are > they any better than the regular superfrost plus? I'm dealing with > frozen lymph node on a Ventana discovery, I've tried a few different > fixing/drying combinations but I still have tissue lifting (not > completely but enough to be bothersome!). Wondering if it's worth > giving the gold slides a go. > > Thanks, > Katy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanbachus <@t> verizon.net Fri Jun 19 15:42:19 2009 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Fri Jun 19 15:42:26 2009 Subject: [Histonet] Superfrost plus gold slides vs superfrost plus In-Reply-To: References: Message-ID: Good old fashioned gelatin subbing is very cheap & works great--I have never seen tissue separate from a subbed slide! Susan ----- Original Message ----- From: "Peggy Bisher" To: "Milne, Katy" ; Sent: Friday, June 19, 2009 3:34 PM Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus >I had a group here who were having trouble with their cryo-sections >sticking > to the slides. Their samples were rat brains. He found that the Gold Super > Frost Plus worked well with in-situ but not for immuno. At least that is > the > comment he wrote down and put on our bulletin board in the lab. Most of > the > people in the lab tend to use the Super Frost Plus (not the Gold) and they > seem pretty happy with them. > > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu > > > > > > On 6/19/09 4:26 PM, "Milne, Katy" wrote: > >> Hi everyone, >> >> I'm curious if anyone has tried using Superfrost plus gold slides, are >> they any better than the regular superfrost plus? I'm dealing with >> frozen lymph node on a Ventana discovery, I've tried a few different >> fixing/drying combinations but I still have tissue lifting (not >> completely but enough to be bothersome!). Wondering if it's worth >> giving the gold slides a go. >> >> Thanks, >> Katy >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.80/2187 - Release Date: 06/19/09 06:53:00 -------------- next part -------------- No virus found in this outgoing message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.80/2187 - Release Date: 06/19/09 06:53:00 From thisisann <@t> aol.com Fri Jun 19 16:01:48 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Jun 19 16:02:05 2009 Subject: [Histonet] Acid Alcohol Message-ID: <8CBBF384BF56786-104C-156C@webmail-mh22.sysops.aol.com> I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? Can someone send it to me? Thanks, Ann From Maria.Katleba <@t> stjoe.org Fri Jun 19 16:12:19 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Jun 19 16:12:31 2009 Subject: [Histonet] Superfrost plus gold slides vs superfrost plus In-Reply-To: References: Message-ID: <97C02552ECB11346877D3E83CF833ABD13CEDE9EE6@SJSNT-SCMAIL03.stjoe.org> But doesn't the gelatin's protein interfere with immuno results? That's why we do not use glue, gelatin, or any adhesive on immuno cases. Maria Katleba HT (ASCP), MS, MBA Pathology Dept. Mgr Queen of the Valley Medical Center Pathology Laboratory Napa Ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Bachus Sent: Friday, June 19, 2009 1:42 PM To: Peggy Bisher; Milne, Katy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus Good old fashioned gelatin subbing is very cheap & works great--I have never seen tissue separate from a subbed slide! Susan ----- Original Message ----- From: "Peggy Bisher" To: "Milne, Katy" ; Sent: Friday, June 19, 2009 3:34 PM Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus >I had a group here who were having trouble with their cryo-sections >sticking > to the slides. Their samples were rat brains. He found that the Gold Super > Frost Plus worked well with in-situ but not for immuno. At least that is > the > comment he wrote down and put on our bulletin board in the lab. Most of > the > people in the lab tend to use the Super Frost Plus (not the Gold) and they > seem pretty happy with them. > > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu > > > > > > On 6/19/09 4:26 PM, "Milne, Katy" wrote: > >> Hi everyone, >> >> I'm curious if anyone has tried using Superfrost plus gold slides, are >> they any better than the regular superfrost plus? I'm dealing with >> frozen lymph node on a Ventana discovery, I've tried a few different >> fixing/drying combinations but I still have tissue lifting (not >> completely but enough to be bothersome!). Wondering if it's worth >> giving the gold slides a go. >> >> Thanks, >> Katy >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.80/2187 - Release Date: 06/19/09 06:53:00 Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From gayle.callis <@t> bresnan.net Fri Jun 19 17:47:55 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jun 19 17:48:12 2009 Subject: [Histonet] Superfrost Gold versus Superfrost Plus Charge Message-ID: <000101c9f12f$fd3f49b0$f7bddd10$@callis@bresnan.net> You did not say whether the tissues were prefixed with formalin or fresh tissues, frozen then cryosectioned? If prefixed, cryoprotected, frozen and cryosectioned, the Plus Gold should work. They are certainly more expensive! A colleague uses Plus Gold for prefixed, cryoprotected frozen sections destined for immunostaining. She sections, then air dries for a time. You may need to determine the time of drying, even if the tissue is fresh. There are package inserts that tell you HOW to use these slides, both Gold or Plus Charge, at least the ones manufactured by Erie have an insert. The Gold slides should be dried flat, but that may be due to contact with a room temperature surface e.g. tray. To help you air dry, purchase a cheap, small fan and dry any FS on Gold or Plus Charge - make sure the air blows pretty much directly onto the sections whether you lay them flat or slanted to be in airflow. You will hasten the drying time and dry more efficiently even in a 30 minute time frame. Try and get a Gold Plus samples from Erie/Thermo Scientific rep in your area, hopefully they still do this. Superfrost Plus Charge (Erie trademarked) works very well with fresh tissue frozen sections, followed by fixation. If we section fresh tissue, air dry a minimum of 30 minutes, solvent fix and stain, we do not lose sections but we also do NOT use an automated staining system. However, if you fix with NBF and use a retrieval method (MW or enzyme digestion) the prefixed or NBF fixed fresh frozen sections may lift off. We never use any kind of gelatin or glue as an adhesive for immunostaining work, just the Plus Charge (trademarked, meaning Erie manufacturered). I have heard the Mercedes coated slides work very well with prefixed tissue frozen sections. You may want to try those too. Since frozen sections are notorious for being more delicate, they cannot withstand some mechanical manipulations such as the reagent dispensing on a stainer - maybe too forceful??? Maybe that can be adjusted so it still allows good flow but not quite so harsh?? If you cut fresh frozen sections, air dry, fix with 4C acetone for 10 min, dry to evaporate solvent and stain, you can stain. A common complaint is poor morphology, but you can post fix a totally IHC frozen section in NBF for 10 min, rinse, then counterstain with hematoxylin. Dr. Chris van der Loos does this with great success and improves the morphology on his section by doing so. Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 From jhaviland <@t> mdanderson.org Fri Jun 19 18:06:12 2009 From: jhaviland <@t> mdanderson.org (Haviland,Joie) Date: Fri Jun 19 18:06:18 2009 Subject: [Histonet] Superfrost plus slides Message-ID: Hello Histonetters: I am having problems with tissue just disappearing in my lastest batches of slides. I have been doing a lot of H&E's and did not have too many problems. But I am cutting testis to use with a CD168 ab. Everytime after the 1mM EDTA AR,the tissue disappears from the slide. I allow the sections to dry overnight in our hood before placing the slides at 60'C overnight. And sometimes I am in a hurry to get it done that I put them right away in the oven. I am also having problems with mouse skin tissue that has hair on it. Seems that the section is lifting on some parts of the slide but not others. It is very frustrating right now. Has anyone else edxperienced this? I have been using the same batch of slides for a couple of weeks.... Thanks Joie From saby_joseph_a <@t> yahoo.com Fri Jun 19 18:59:06 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Fri Jun 19 18:59:10 2009 Subject: [Histonet] Acid Alcohol In-Reply-To: <8CBBF384BF56786-104C-156C@webmail-mh22.sysops.aol.com> References: <8CBBF384BF56786-104C-156C@webmail-mh22.sysops.aol.com> Message-ID: <62566.54499.qm@web33808.mail.mud.yahoo.com> 1% Nitric acid in 70% ethanbol. Joe Saby ________________________________ From: "thisisann@aol.com" To: histonet@lists.utsouthwestern.edu Sent: Friday, June 19, 2009 5:01:48 PM Subject: [Histonet] Acid Alcohol I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? Can someone send it to me? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Jun 19 19:56:59 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jun 19 19:57:11 2009 Subject: [Histonet] Superfrost plus slides In-Reply-To: References: Message-ID: <000001c9f142$051b71b0$0f525510$@callis@bresnan.net> You did not say which slides you use, but you may want to try Thermo Scientific EXCELL slides. The special coating resists the effects of high pH EDTA antigen retrieval and heat. Hopefully, you are putting any adhesives in the water-bath when using Plus Charge or any other coated slides. The gelatin in these adhesives coats the plus charge and negates that charge. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Haviland,Joie Sent: Friday, June 19, 2009 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Superfrost plus slides Hello Histonetters: I am having problems with tissue just disappearing in my lastest batches of slides. I have been doing a lot of H&E's and did not have too many problems. But I am cutting testis to use with a CD168 ab. Everytime after the 1mM EDTA AR,the tissue disappears from the slide. I allow the sections to dry overnight in our hood before placing the slides at 60'C overnight. And sometimes I am in a hurry to get it done that I put them right away in the oven. I am also having problems with mouse skin tissue that has hair on it. Seems that the section is lifting on some parts of the slide but not others. It is very frustrating right now. Has anyone else edxperienced this? I have been using the same batch of slides for a couple of weeks.... Thanks Joie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Jun 19 19:58:50 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jun 19 19:59:03 2009 Subject: [Histonet] Acid Alcohol In-Reply-To: <62566.54499.qm@web33808.mail.mud.yahoo.com> References: <8CBBF384BF56786-104C-156C@webmail-mh22.sysops.aol.com> <62566.54499.qm@web33808.mail.mud.yahoo.com> Message-ID: <000101c9f142$47a2ffd0$d6e8ff70$@callis@bresnan.net> We have used 0.5% to 1% HCl in 70% alcohoL but only for Harris regressive hematoxylin staining. For progressive hematoxylins, and Gills, 1% acetic acid was preferred. Gayle M. Callis HTL(ASCP)HT,MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Friday, June 19, 2009 5:59 PM To: thisisann@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Acid Alcohol 1% Nitric acid in 70% ethanbol. Joe Saby ________________________________ From: "thisisann@aol.com" To: histonet@lists.utsouthwestern.edu Sent: Friday, June 19, 2009 5:01:48 PM Subject: [Histonet] Acid Alcohol I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? Can someone send it to me? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lynnd01 <@t> hotmail.com Fri Jun 19 20:52:49 2009 From: lynnd01 <@t> hotmail.com (Lynn Dike) Date: Fri Jun 19 20:52:54 2009 Subject: FW: [Histonet] xylene and frozen sections In-Reply-To: References: Message-ID: Lynn From: lynnd01@hotmail.com To: lynnd01@hotmail.com Subject: RE: [Histonet] xylene and frozen sections Date: Thu, 18 Jun 2009 19:53:52 -0500 Is there any literature that says xylene will fade progressive staining H&E (using Surgipath Harris Hematoxylin) over a period of years? Our frozen staining series after the eosin has two 95% ROH's and six 100% and then clear in Propar. I mistakenly replaced the last three 100% ROH with xylene when I rotated the series. I'm told that anyone who knows anything about chemistry knows this is a big deal and the sections will fade. Every lab I've worked in used xylene to clear frozen sections. I understand that I messed up because this isn't our proceedure, but I don't believe I sacrificed patient care. Am I wrong? Lynn > From: lynnd01@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 18 Jun 2009 19:47:14 -0500 > Subject: [Histonet] xylene and frozen sections > > > > > > Lynn > > > _________________________________________________________________ > Insert movie times and more without leaving Hotmail?. > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 Insert movie times and more without leaving Hotmail?. See how. _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009 From cmiller <@t> gladstone.ucsf.edu Sat Jun 20 00:01:25 2009 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Sat Jun 20 00:02:15 2009 Subject: [Histonet] Acid Alcohol In-Reply-To: <8CBBF384BF56786-104C-156C@webmail-mh22.sysops.aol.com> References: <8CBBF384BF56786-104C-156C@webmail-mh22.sysops.aol.com> Message-ID: <1DC64805-7810-4B8F-B370-1E2B5FCD50E3@gladstone.ucsf.edu> 1% cHCl in 70% ethanol (reagent alcohol), couple of seconds is plenty Caroline Caroline Miller M.Sc DIC AIBMS Co-manager Gladstone Microscopy and Histology Core J David Gladstone institute 1650 Owens Street San Francisco CA 94158 www.gladstone.ucsf.edu On Jun 19, 2009, at 17:01, thisisann@aol.com wrote: > I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? > Can someone send it to me? > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Jun 20 04:38:29 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jun 20 04:38:35 2009 Subject: AW: [Histonet] xylene and frozen sections In-Reply-To: References: Message-ID: <879D06EC5A1146949236434CF298A682@dielangs.at> I've never read about fading caused by xylene. What about all people, who uses the film-coverslipper? That instruments demands on xylene as clearant. So if it would be true, there would be many faded slides in the archives. Perhaps your colleagues thought, that dehydration wasn't sufficient and the remaining water could cause fading. But after three 100% ethanol, I think, this fear has no cause. We use only one 96 and two 100 for the frozens and have never had problems. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lynn Dike Gesendet: Samstag, 20. Juni 2009 03:53 An: histonet@lists.utsouthwestern.edu Betreff: FW: [Histonet] xylene and frozen sections Lynn From: lynnd01@hotmail.com To: lynnd01@hotmail.com Subject: RE: [Histonet] xylene and frozen sections Date: Thu, 18 Jun 2009 19:53:52 -0500 Is there any literature that says xylene will fade progressive staining H&E (using Surgipath Harris Hematoxylin) over a period of years? Our frozen staining series after the eosin has two 95% ROH's and six 100% and then clear in Propar. I mistakenly replaced the last three 100% ROH with xylene when I rotated the series. I'm told that anyone who knows anything about chemistry knows this is a big deal and the sections will fade. Every lab I've worked in used xylene to clear frozen sections. I understand that I messed up because this isn't our proceedure, but I don't believe I sacrificed patient care. Am I wrong? Lynn > From: lynnd01@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 18 Jun 2009 19:47:14 -0500 > Subject: [Histonet] xylene and frozen sections > > > > > > Lynn > > > _________________________________________________________________ > Insert movie times and more without leaving Hotmail?. > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009 Insert movie times and more without leaving Hotmail?. See how. _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutoria l_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sun Jun 21 10:59:38 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sun Jun 21 11:01:41 2009 Subject: [Histonet] re:xylene and frozen sections Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0EEB@KCL-MAIL04.kclad.ds.kcl.ac.uk> Imho, if one does not use Xylene that is low in sulphur, there is a good chance that your eosin staining will fade, whether on frozen or Pwax sections... carl From laurie.reilly <@t> jcu.edu.au Sun Jun 21 18:19:49 2009 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Sun Jun 21 18:22:43 2009 Subject: [Histonet] sections fading In-Reply-To: <11D9615B89C10747B1C985966A63D7CA2961FE0EEB@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA2961FE0EEB@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: Talking of sections fading -- I am having trouble with my Grams fading. We do Gram Twort on paraffin sections and they stain beautifully, but after 3 or 4 days the Gram positive bugs are no longer Gram positive. Can anyone help? Thanks and regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Monday, 22 June 2009 2:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re:xylene and frozen sections Imho, if one does not use Xylene that is low in sulphur, there is a good chance that your eosin staining will fade, whether on frozen or Pwax sections... carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rhbrown1 <@t> histocs.com Sun Jun 21 22:08:58 2009 From: rhbrown1 <@t> histocs.com (Leroy Brown) Date: Sun Jun 21 22:11:21 2009 Subject: [Histonet] help needed with Tissue-Tek DRS2000 Message-ID: Hi, I have recently acquired a Tissue-Tek DRS 2000 and I am wondering if there would be someone I could chat with about setup and operation of this stainer. If you would be willing to share your knowledge on this machine please let me know. My phone number is 360-966-7300. Thanks, LeRoy Brown HT(ASCP) HTL Everson, Wa. 98247 Histocs.com From cls71877 <@t> sbcglobal.net Sun Jun 21 22:51:34 2009 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Sun Jun 21 22:51:38 2009 Subject: [Histonet] Leica ASP 300 Message-ID: <207327.79612.qm@web81201.mail.mud.yahoo.com> Hello Histonetters, I was wondering if anyone has a processing schedule for strictly GI biopsies on the Leica?ASP 300.? Maybe even a rapid processing schedule? Thanks in advance for your time! Cristi From jessgrocki <@t> yahoo.com Mon Jun 22 10:16:21 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Mon Jun 22 10:16:24 2009 Subject: [Histonet] DFA- Pneumocystis Message-ID: <969772.25698.qm@web82008.mail.mud.yahoo.com> ? Good Morning All, ? Just wondering where people are getting there positive control slides for DFA/Pneumocystis stain? We need to have smeared positive controls. We are doing the stain on BAL slides (cytospins).?Does anyone know where we can get them? ? ?Thank you, ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ? ? From jessgrocki <@t> yahoo.com Mon Jun 22 10:18:37 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Mon Jun 22 10:18:43 2009 Subject: [Histonet] Diff Quik II Message-ID: <91603.74687.qm@web82005.mail.mud.yahoo.com> ? Just wanted to say thanks to everyone who shared their knowledge with me regarding the Diff Quik II. ? Have a great day! Jessica Piche-Grocki, HT(ASCP) From luke.perkocha <@t> ucsf.edu Mon Jun 22 11:41:19 2009 From: luke.perkocha <@t> ucsf.edu (Perkocha, Luke) Date: Mon Jun 22 11:41:25 2009 Subject: [Histonet] Pathology to OR Communication System Message-ID: <151F5A3C6F4AD44083C9DA960250CF200DD2A8C110@EX01.net.ucsf.edu> Hello All, We have a very old speakerphone system that we use to call from the pathologist's office in to our operating rooms to discuss frozen section diagnoses with the surgeons. Both sides are yelling and straining to hear and we're concerned about the risk for miscommunication. We can't go into the OR directly, since Pathology and the OR are too far apart physically. We're looking for some sort of telephone-based communications system, perhaps with a speaker and microphone that can be mounted near the surgeon, so that when we call into the OR with the frozen section diagnosis, it can be switched to speaker and the call can be continued with direct and audible communication between the pathologist on the phone in his/her office and the surgical team at the head of the operating table. Does anyone out there have a system like this? Do you know of a commercial vendor who makes something that would work? We tried an expensive "Polycom" system meant for conference calls, but its 360 degree microphones picked up too much background noise in the OR. It seems like it should be a simple Radio Shack project, but we're all techno-challenged. Any help would be appreciated. Thanks. Luke Perkocha Luke.perkocha@ucsf.edu From Maria.Katleba <@t> stjoe.org Mon Jun 22 13:51:45 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Jun 22 13:51:52 2009 Subject: [Histonet] RE: Pathology to OR Communication System In-Reply-To: <151F5A3C6F4AD44083C9DA960250CF200DD2A8C110@EX01.net.ucsf.edu> References: <151F5A3C6F4AD44083C9DA960250CF200DD2A8C110@EX01.net.ucsf.edu> Message-ID: <97C02552ECB11346877D3E83CF833ABD13CEDEA33A@SJSNT-SCMAIL03.stjoe.org> Hi Luke, We are currently working with architects on designing the new lab we are building. This particular item is one of the projects we are working on. I will let you know how we decide to have connectivity to OR. Remember, that is you spend just a little more, you can us a CSTV close circuit tv. This way OR can see how the pathologist ink & orient the specimen! I will keep you posted. Its not as expensive as you may think. Maria Katleba HT(ASCP) MS Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perkocha, Luke Sent: Monday, June 22, 2009 9:41 AM To: 'histonet@lists.utsouthwestern.edu'; 'patho-l@mailman.srv.ualberta.ca'; 'MEDLAB-L@listserv.buffalo.edu' Subject: [Histonet] Pathology to OR Communication System Hello All, We have a very old speakerphone system that we use to call from the pathologist's office in to our operating rooms to discuss frozen section diagnoses with the surgeons. Both sides are yelling and straining to hear and we're concerned about the risk for miscommunication. We can't go into the OR directly, since Pathology and the OR are too far apart physically. We're looking for some sort of telephone-based communications system, perhaps with a speaker and microphone that can be mounted near the surgeon, so that when we call into the OR with the frozen section diagnosis, it can be switched to speaker and the call can be continued with direct and audible communication between the pathologist on the phone in his/her office and the surgical team at the head of the operating table. Does anyone out there have a system like this? Do you know of a commercial vendor who makes something that would work? We tried an expensive "Polycom" system meant for conference calls, but its 360 degree microphones picked up too much background noise in the OR. It seems like it should be a simple Radio Shack project, but we're all techno-challenged. Any help would be appreciated. Thanks. Luke Perkocha Luke.perkocha@ucsf.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From pruegg <@t> ihctech.net Mon Jun 22 14:53:14 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jun 22 14:53:15 2009 Subject: [Histonet] Leica CV5030 Message-ID: I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From joseph-galbraith <@t> uiowa.edu Mon Jun 22 15:16:52 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Jun 22 15:16:58 2009 Subject: [Histonet] RE: Pathology to OR Communication System In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13CEDEA33A@SJSNT-SCMAIL03.stjoe.org> References: <151F5A3C6F4AD44083C9DA960250CF200DD2A8C110@EX01.net.ucsf.edu> <97C02552ECB11346877D3E83CF833ABD13CEDEA33A@SJSNT-SCMAIL03.stjoe.org> Message-ID: Luke: I would concur with Maria. Years ago we installed a two-way CCTV system between the Surgical Path Gross Room and the OR's. Via video cameras above the grossing stations and on the microscope and above the OR table, the pathologist and the surgeon can have two-way video and audio communication. We did find that the audio portion was not as clear as we would have liked so we actually just call into the OR room and converse with the surgeon over the telephone. While the whole two-way video is a neat feature, most surgeons only really need to hear a verbal diagnosis. But it does come in handy for those surgeons who want to see the specimen and/or the frozen section stained slide and simultaneously discuss the diagnosis with the pathologist during times when the surgeon can not leave the table. (For some of the marathon surgeries that we have, the surgeon will occasionally come to the gross room and look directly at the specimen or microscope.) Good luck. Joe 380 MRC 4-4737 (voice) 5-8453 (fax) pager 131-1170 joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Monday, June 22, 2009 1:52 PM To: Perkocha, Luke; 'histonet@lists.utsouthwestern.edu'; 'patho-l@mailman.srv.ualberta.ca'; 'MEDLAB-L@listserv.buffalo.edu' Subject: [Histonet] RE: Pathology to OR Communication System Hi Luke, We are currently working with architects on designing the new lab we are building. This particular item is one of the projects we are working on. I will let you know how we decide to have connectivity to OR. Remember, that is you spend just a little more, you can us a CSTV close circuit tv. This way OR can see how the pathologist ink & orient the specimen! I will keep you posted. Its not as expensive as you may think. Maria Katleba HT(ASCP) MS Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perkocha, Luke Sent: Monday, June 22, 2009 9:41 AM To: 'histonet@lists.utsouthwestern.edu'; 'patho-l@mailman.srv.ualberta.ca'; 'MEDLAB-L@listserv.buffalo.edu' Subject: [Histonet] Pathology to OR Communication System Hello All, We have a very old speakerphone system that we use to call from the pathologist's office in to our operating rooms to discuss frozen section diagnoses with the surgeons. Both sides are yelling and straining to hear and we're concerned about the risk for miscommunication. We can't go into the OR directly, since Pathology and the OR are too far apart physically. We're looking for some sort of telephone-based communications system, perhaps with a speaker and microphone that can be mounted near the surgeon, so that when we call into the OR with the frozen section diagnosis, it can be switched to speaker and the call can be continued with direct and audible communication between the pathologist on the phone in his/her office and the surgical team at the head of the operating table. Does anyone out there have a system like this? Do you know of a commercial vendor who makes something that would work? We tried an expensive "Polycom" system meant for conference calls, but its 360 degree microphones picked up too much background noise in the OR. It seems like it should be a simple Radio Shack project, but we're all techno-challenged. Any help would be appreciated. Thanks. Luke Perkocha Luke.perkocha@ucsf.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Mon Jun 22 20:44:31 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Jun 22 20:45:37 2009 Subject: [Histonet] Pathology to OR Communication System In-Reply-To: <151F5A3C6F4AD44083C9DA960250CF200DD2A8C110@EX01.net.ucsf.edu> References: <151F5A3C6F4AD44083C9DA960250CF200DD2A8C110@EX01.net.ucsf.edu> Message-ID: <4A40C01E.471C.0039.0@health.qld.gov.au> Hi Luke A simple cheap solution would be to buy telephones for the OR and the lab that have speaker phone capabilities. No expensive purchase cost or installation required. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Perkocha, Luke" 23/06/2009 2:41 am >>> Hello All, We have a very old speakerphone system that we use to call from the pathologist's office in to our operating rooms to discuss frozen section diagnoses with the surgeons. Both sides are yelling and straining to hear and we're concerned about the risk for miscommunication. We can't go into the OR directly, since Pathology and the OR are too far apart physically. We're looking for some sort of telephone-based communications system, perhaps with a speaker and microphone that can be mounted near the surgeon, so that when we call into the OR with the frozen section diagnosis, it can be switched to speaker and the call can be continued with direct and audible communication between the pathologist on the phone in his/her office and the surgical team at the head of the operating table. Does anyone out there have a system like this? Do you know of a commercial vendor who makes something that would work? We tried an expensive "Polycom" system meant for conference calls, but its 360 degree microphones picked up too much background noise in the OR. It seems like it should be a simple Radio Shack project, but we're all techno-challenged. Any help would be appreciated. Thanks. Luke Perkocha Luke.perkocha@ucsf.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From mpence <@t> grhs.net Tue Jun 23 08:13:48 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jun 23 08:13:55 2009 Subject: [Histonet] Pathology to OR Communication System In-Reply-To: <4A40C01E.471C.0039.0@health.qld.gov.au> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3B88@IS-E2K3.grhs.net> We have this and hate it! The speaker phone does not allow for bi-directional conversations. The pathologist may report to the room that he/she sees no tumor in section examined and the OR may only hear tumor in sections examined. This is not a good thing! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Monday, June 22, 2009 8:45 PM To: 'histonet@lists.utsouthwestern.edu'; 'MEDLAB-L@listserv.buffalo.edu'; 'patho-l@mailman.srv.ualberta.ca'; Luke Perkocha Subject: Re: [Histonet] Pathology to OR Communication System Hi Luke A simple cheap solution would be to buy telephones for the OR and the lab that have speaker phone capabilities. No expensive purchase cost or installation required. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Perkocha, Luke" 23/06/2009 2:41 am >>> Hello All, We have a very old speakerphone system that we use to call from the pathologist's office in to our operating rooms to discuss frozen section diagnoses with the surgeons. Both sides are yelling and straining to hear and we're concerned about the risk for miscommunication. We can't go into the OR directly, since Pathology and the OR are too far apart physically. We're looking for some sort of telephone-based communications system, perhaps with a speaker and microphone that can be mounted near the surgeon, so that when we call into the OR with the frozen section diagnosis, it can be switched to speaker and the call can be continued with direct and audible communication between the pathologist on the phone in his/her office and the surgical team at the head of the operating table. Does anyone out there have a system like this? Do you know of a commercial vendor who makes something that would work? We tried an expensive "Polycom" system meant for conference calls, but its 360 degree microphones picked up too much background noise in the OR. It seems like it should be a simple Radio Shack project, but we're all techno-challenged. Any help would be appreciated. Thanks. Luke Perkocha Luke.perkocha@ucsf.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ******** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ************************************************************************ ********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Jun 23 08:51:43 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jun 23 08:51:47 2009 Subject: [Histonet] Leica CV5030 In-Reply-To: Message-ID: We are very pleased with this instrument. It was originally purchased as a stand-alone unit. A few years later we were able to purchase the ST5020 stainer to connect to it. The technical staff at Leica has been great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Monday, June 22, 2009 3:53 PM To: 'Histonet' Subject: [Histonet] Leica CV5030 I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. And, find us on Facebook and "Become a Fan" for up-to-the-minute medical center news. From HornHV <@t> archildrens.org Tue Jun 23 09:16:30 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jun 23 09:16:35 2009 Subject: [Histonet] Leica CV5030 In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8329B@EMAIL.archildrens.org> We absolutely love our CV5030. I really can't think of anything negative to say about it. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, June 22, 2009 2:53 PM To: 'Histonet' Subject: [Histonet] Leica CV5030 I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From esther.peters <@t> verizon.net Tue Jun 23 09:35:49 2009 From: esther.peters <@t> verizon.net (Esther Peters) Date: Tue Jun 23 09:36:04 2009 Subject: [Histonet] Clearing agent advice Message-ID: <4A40E845.9040201@verizon.net> Have any of you used Clearify, Naturalene, or Master Clear? I have been using SafeClear II, but need to change. Which of these might be most like SafeClear? Will they all work with Permount? Thank you very much for your insights! Esther Peters, Ph.D. Assistant Professor George Mason University From rhbrown1 <@t> histocs.com Tue Jun 23 09:38:49 2009 From: rhbrown1 <@t> histocs.com (Leroy Brown) Date: Tue Jun 23 09:41:25 2009 Subject: [Histonet] service manual Message-ID: Hi, I need to acquire a service manual for a stainer I just picked up to use in my lab. It is the Tissue-tek DRS 2000 and I wonder if anyone has one and would be willing to make a copy of for me. I know they do have new ones but because they are very expensive -250.00. I can not afford to buy it. This is not the operation manual, I have that already, it is the service manual. Also, I am just learning to use this stainer so if there is anyone out there in histo-land who has one of these DRS2000 stainers, who would be willing to contact me about how to set this machine up, I would be very grateful too be able to speak to you. I would be able to pay you for your time and effort for the service manual copy. Thanks in advance. LeRoy Brown HT(ASCP)HTL Everson, WA 98247 360-966-7300 From gvdobbin <@t> ihis.org Tue Jun 23 09:41:47 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Jun 23 09:42:18 2009 Subject: [Histonet] Leica CV5030 Message-ID: Hi Patsy, I will concur with Hazel but would add that the coverslipper has only been hassle free for us as long we are using the better quality (ie "Premium" grade) coverslips. Cheaper coverslips are not economical when one considers the time lost to fix and reset the instrument and in some cases re-coverslip slides. But since I switched to using the premium grade year round (as opposed to only in the more humid months, which is what I was doing to try andsave money!) I have had almost no glitches at all! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Horn, Hazel V" 6/23/2009 11:16 AM >>> We absolutely love our CV5030. I really can't think of anything negative to say about it. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, June 22, 2009 2:53 PM To: 'Histonet' Subject: [Histonet] Leica CV5030 I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From trathborne <@t> somerset-healthcare.com Tue Jun 23 09:47:10 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jun 23 09:47:17 2009 Subject: [Histonet] Leica CV5030 In-Reply-To: Message-ID: We actually had our Leica rep suggest a vendor for coverglass. They are not as expensive as many others, and they work great. Stat Lab, catalog 102450E, 2 oz or 102450 for the traditional 1 oz/per box. http://www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Greg Dobbin Sent: Tuesday, June 23, 2009 10:42 AM To: HornHV@archildrens.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica CV5030 Hi Patsy, I will concur with Hazel but would add that the coverslipper has only been hassle free for us as long we are using the better quality (ie "Premium" grade) coverslips. Cheaper coverslips are not economical when one considers the time lost to fix and reset the instrument and in some cases re-coverslip slides. But since I switched to using the premium grade year round (as opposed to only in the more humid months, which is what I was doing to try andsave money!) I have had almost no glitches at all! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Horn, Hazel V" 6/23/2009 11:16 AM >>> We absolutely love our CV5030. I really can't think of anything negative to say about it. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, June 22, 2009 2:53 PM To: 'Histonet' Subject: [Histonet] Leica CV5030 I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. And, find us on Facebook and "Become a Fan" for up-to-the-minute medical center news. From billodonnell <@t> catholichealth.net Tue Jun 23 09:55:06 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Jun 23 09:55:17 2009 Subject: [Histonet] Budget Microarray - Finally In-Reply-To: References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> Message-ID: Good day Histonetters, A few weeks back, I posted that I had a method for making a tissue microarray on the cheap. I have received a lot of requests for how I do this. I wanted to write it up because, even though it is pretty easy to do, it is difficult to describe. So...I made a PowerPoint on how to do it and posted it at this address: http://highperformancehistology.yolasite.com/ With your indulgence I will re-post a few times iin the next couple of weeks so that those who contacted me won't miss out. (I suppose I could have saved all those addresses...ah, the wonder of hindsight! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From djohnson <@t> mercedesmedical.com Tue Jun 23 09:54:55 2009 From: djohnson <@t> mercedesmedical.com (Dave Johnson) Date: Tue Jun 23 09:55:30 2009 Subject: [Histonet] Leica CV5030 In-Reply-To: References: Message-ID: Not sure if sending attachments is bad. Following is a letter from Leica recommending several coverslips for their automated machine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, June 23, 2009 10:47 AM To: Greg Dobbin; HornHV@archildrens.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica CV5030 We actually had our Leica rep suggest a vendor for coverglass. They are not as expensive as many others, and they work great. Stat Lab, catalog 102450E, 2 oz or 102450 for the traditional 1 oz/per box. http://www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Greg Dobbin Sent: Tuesday, June 23, 2009 10:42 AM To: HornHV@archildrens.org; pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica CV5030 Hi Patsy, I will concur with Hazel but would add that the coverslipper has only been hassle free for us as long we are using the better quality (ie "Premium" grade) coverslips. Cheaper coverslips are not economical when one considers the time lost to fix and reset the instrument and in some cases re-coverslip slides. But since I switched to using the premium grade year round (as opposed to only in the more humid months, which is what I was doing to try andsave money!) I have had almost no glitches at all! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Horn, Hazel V" 6/23/2009 11:16 AM >>> We absolutely love our CV5030. I really can't think of anything negative to say about it. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, June 22, 2009 2:53 PM To: 'Histonet' Subject: [Histonet] Leica CV5030 I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. 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If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. And, find us on Facebook and "Become a Fan" for up-to-the-minute medical center news. From victor <@t> pathology.washington.edu Tue Jun 23 10:05:08 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jun 23 10:05:13 2009 Subject: [Histonet] Budget Microarray - Finally In-Reply-To: References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> Message-ID: <4A40EF24.9050501@pathology.washington.edu> Bill, Very nice and thanks for sharing. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. O'Donnell, Bill wrote: > > Good day Histonetters, > > A few weeks back, I posted that I had a method for making a tissue > microarray on the cheap. I have received a lot of requests for how I do > this. I wanted to write it up because, even though it is pretty easy to > do, it is difficult to describe. > > So...I made a PowerPoint on how to do it and posted it at this address: > > http://highperformancehistology.yolasite.com/ > > With your indulgence I will re-post a few times iin the next couple of > weeks so that those who contacted me won't miss out. (I suppose I could > have saved all those addresses...ah, the wonder of hindsight! > > William (Bill) O'Donnell, HT (ASCP) QIHC > Lead Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cdemarinis <@t> SARATOGACARE.ORG Tue Jun 23 10:28:11 2009 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Tue Jun 23 10:28:18 2009 Subject: [Histonet] Leica CM1850uv Message-ID: We purchased a Leica CM1850uv cryostat in 2007 and we constantly have problems with buildup of ice on the specimen metal bar. This causes the tissue to stick to the stationary heat extractor which causes significant problems cutting frozen sections. The engineer has serviced the machine several times, but recognizes this as "normal ice buildup". We manually defrost the unit 2-3 times a day, but it does not seem to help. Is anyone else experiencing the same problem, and if so, what are they doing about it? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From king.laurie <@t> marshfieldclinic.org Tue Jun 23 10:37:29 2009 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Tue Jun 23 10:37:37 2009 Subject: [Histonet] Leica CM1850uv Message-ID: <200906231537.n5NFbVvh008525@mailhost2.mfldclin.edu> I've worked with 4 of these units, two had the problem, two didn't. Long story short, the problem won't go away until you have the Peltier unit replaced. That is not normal ice buildup. Laurie ------Original Message------ From: "Demarinis, Carolyn" Date: Tue Jun 23, 2009 -- 10:29:08 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Leica CM1850uv We purchased a Leica CM1850uv cryostat in 2007 and we constantly have problems with buildup of ice on the specimen metal bar. This causes the tissue to stick to the stationary heat extractor which causes significant problems cutting frozen sections. The engineer has serviced the machine several times, but recognizes this as "normal ice buildup". We manually defrost the unit 2-3 times a day, but it does not seem to help. Is anyone else experiencing the same problem, and if so, what are they doing about it? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly_colpitts <@t> hotmail.com Tue Jun 23 10:47:44 2009 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Tue Jun 23 10:47:48 2009 Subject: [Histonet] Positive Patient ID Message-ID: Hi Histoland, Recently we had a CAP inspection. One of the deficiencies that we were sited for was a lack of positive patient ID. Here's is our current process (the process that has been in place forever and a day). 1)Specimens are collected. 2)They are assigned the next specimen numbers (the next available number is recorded on a steno pad next to the cassette labeler as well as stored in the cassette labeler). 3)Cassettes are made and the specimen, cassettes and a copy of the paper requisition are given to the pathologist to be grossed in. 4)Once all specimens have assigned numbers and have been given to pathologist, we enter the information into our LIS (Cerner Classic). While we would love to be able to afford to purchase one of the many positive patient ID barcode systems, this isn't feasible at this time. So I am wondering what other labs are doing to comply with positive patient ID? Thanks for all your help! Kelly _________________________________________________________________ Bing? brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1 From Janet.Bonner <@t> FLHOSP.ORG Tue Jun 23 10:57:41 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jun 23 10:57:55 2009 Subject: [Histonet] Leica CM1850uv References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2A6C@fhosxchmb006.ADVENTISTCORP.NET> I also am using the CM1850, we have several of them in our hospital system. We do not have ice build-up at all in any of them, and we are in a VERY HUMID and VERY HOT Florida. I would suggest using another service rep. Where are you located? I could recommend a great service company in my area. As a desperate, short-term measure, you could put a cup of desiccant on the back of the work-area plate. Don't let the Turkeys get you down! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Demarinis, Carolyn Sent: Tue 6/23/2009 11:28 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Leica CM1850uv We purchased a Leica CM1850uv cryostat in 2007 and we constantly have problems with buildup of ice on the specimen metal bar. This causes the tissue to stick to the stationary heat extractor which causes significant problems cutting frozen sections. The engineer has serviced the machine several times, but recognizes this as "normal ice buildup". We manually defrost the unit 2-3 times a day, but it does not seem to help. Is anyone else experiencing the same problem, and if so, what are they doing about it? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From rsrichmond <@t> gmail.com Tue Jun 23 11:06:58 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jun 23 11:07:03 2009 Subject: [Histonet] Re: Pathology to OR Communication System Message-ID: In the many surgical pathology services I've worked in as a locum tenens pathologist, I have almost never seen anything but a squawk-box system for communicating with the surgeon in the OR. Communication fails if the OR is noisy or if the surgeon doesn't speak English very well. Often I wind up talking to an illiterate circulator. I suppose this arrangement is prescribed in the Hospital Administrator's Top-Secret Handy-Dandy Book on How to Make Life Hard for the Pathologist. (I am deeply convinced such a document exists, since I see exactly the same problems in multiple pathology services.) What I don't understand is - presumably surgeons in the OR rather often need telephone communication with the outside world - how do they do it for important communications, like about a golf game or a hot market tip? I'd want to find this out if I were setting up a system. I never thought of a video link. It would indeed be useful for orienting skin specimens, but adequate macro magnification would be essential, since these specimens are often quite small. Photomicrography would require still another set-up. The traditional frozen section set-up in the operative suite would require all duplicate equipment. We've had this problem for years with microscopes - the worst microscope in the lab is always relegated to the FS suite. I'd want to do the frozen sections in the pathology lab, if I were setting up a new system and could have my druthers. Bob Richmond Samurai Pathologist Knoxville TN From Janet.Bonner <@t> FLHOSP.ORG Tue Jun 23 11:06:46 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jun 23 11:07:06 2009 Subject: [Histonet] Leica CV5030 References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2A6D@fhosxchmb006.ADVENTISTCORP.NET> Leica recommends using the xylene-based coverslipping media, although it is more costly than the Toluene-based coverslipping media. So we used the toluene-based media and had some problems with the needle clogging up, but then we are a 24/7 lab with nearly 2000 slides per 24 hours! We absolutely love these coverslippers. We do use specialized coverglass. But it's like a truck: If you can't afford the gas, don't buy the truck! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patsy Ruegg Sent: Mon 6/22/2009 3:53 PM To: 'Histonet' Subject: [Histonet] Leica CV5030 I am looking for experiences with this coverslipper from Leica CV5030 model, good or bad. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From gayle.callis <@t> bresnan.net Tue Jun 23 11:19:43 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Jun 23 11:20:02 2009 Subject: [Histonet] RE: Ice build up on Message-ID: <000701c9f41e$6c313450$44939cf0$@callis@bresnan.net> You wrote: We purchased a Leica CM1850uv cryostat in 2007 and we constantly have problems with buildup of ice on the specimen metal bar. This causes the tissue to stick to the stationary heat extractor which causes significant problems cutting frozen sections. The engineer has serviced the machine several times, but recognizes this as "normal ice buildup". We manually defrost the unit 2-3 times a day, but it does not seem to help. Is anyone else experiencing the same problem, and if so, what are they doing about it? Thank you. Re: By manually defrosting the "unit", do you mean using the mini-defrost command for the cold bar area and not the whole cryostat? We have used that command to clear ice from holes but it tends to get really hot. After this is done, the melted ice water is wiped away with 100% alcohol damp gauze IF the water is apparent/obvious since it will simply refreeze and cause ice buildup again. Consequently, we have not used that mini defrost mode too often but rather do the following. To get rid of the ice out of little holes and off cold bar, use a Q tip dampened with 95% alcohol in holes and a 95% dampened gauze on flat surfaces. Since alcohol is an antifreeze we can eliminate the ice without heating up the bar area. Also wipe the underside of the heat extractor to get rid of any ice crystal build up located there. If 95% is not working well, use 70% followed by 95% or 100% to get rid of any residual water left from the 70%. The main thing is to clean the bottom of heat extractor more often and just before freezing since the ice crystals are messing up cryotomy. Just don't get alcohol on your tissue by wiping underside with dry, RT gauze after the alcohol wipe. One, be sure to minimize adding water to the cryostat - sometimes difficult. This can happen when cleaning with 70% alcohol that is dripping off gauze or normal usage. If one dampens the gauze for cleaning and wipes down interior - buildup is minimized. If you see too much 70% then wipe again with 100% to help dry the surfaces before you turn on the UV light. Also, major defrosting of whole cryostat may have to be done more frequently. We raise the removable metal plate and check for excessive ice buildup under the and the back of chamber and defrost the whole cryostat if the ice begins to look like our Montana winter. We live in such a dry climate that frost buildup is minimal, but high humidity days are factor too. Merely suggestions and good luck Gayle M. Callis HTL(ASCP) HT, MT Bozeman MT From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jun 23 11:23:19 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jun 23 11:23:29 2009 Subject: [Histonet] Leica CM1850uv In-Reply-To: Message-ID: Carolyn Give me a call I we can arrange to have a service call to repair your instrument. This can be repaired. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Demarinis, Carolyn" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Leica CM1850uv 06/23/2009 10:28 AM We purchased a Leica CM1850uv cryostat in 2007 and we constantly have problems with buildup of ice on the specimen metal bar. This causes the tissue to stick to the stationary heat extractor which causes significant problems cutting frozen sections. The engineer has serviced the machine several times, but recognizes this as "normal ice buildup". We manually defrost the unit 2-3 times a day, but it does not seem to help. Is anyone else experiencing the same problem, and if so, what are they doing about it? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cbrya <@t> lexclin.com Tue Jun 23 09:26:22 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Tue Jun 23 11:37:46 2009 Subject: [Histonet] tissue processor Message-ID: <37A1F9CAB9E21C41B39F3653B620D13E093BA3DE@exchange2003.lc.local> If you were going to purchase a new tissue processor, which would you recommend for a small lab that runs ? 200 blocks per day? ?The majority of our workload is biopsies (skins & GI) with a few breast masses. ?We also do a fair volume of IHC and are reluctant to go to microwave processing for this reason. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by the State of Kentucky and/or Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient and have received this communication in error, please contact the sender and destroy all copies of the original message. Thank you. From wdesalvo.cac <@t> hotmail.com Tue Jun 23 12:25:07 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Jun 23 12:25:12 2009 Subject: [Histonet] Positive Patient ID In-Reply-To: References: Message-ID: Did you specifically ask what the inspectors wanted you to do to correct the deficiency? I cannot speak for the inspectors, but I believe the issue associated w/ your deficiency is that the patient and accession number are not entered into the LIS at the beginning of your process. not that you need an expensive barcoding system. There are multiple opportunities for duplication of accession numbers and a lack of tracking the case through your process. Again, I suggest you have a detaialed conversation with the inspectors to understand their reasoning for issuing the deficiency. William DeSalvo, B.S., HTL(ASCP) > From: kelly_colpitts@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 23 Jun 2009 11:47:44 -0400 > Subject: [Histonet] Positive Patient ID > > > Hi Histoland, > Recently we had a CAP inspection. One of the deficiencies that we were sited for was a lack of positive patient ID. Here's is our current process (the process that has been in place forever and a day). > 1)Specimens are collected. > 2)They are assigned the next specimen numbers (the next available number is recorded on a steno pad next to the cassette labeler as well as stored in the cassette labeler). > 3)Cassettes are made and the specimen, cassettes and a copy of the paper requisition are given to the pathologist to be grossed in. > 4)Once all specimens have assigned numbers and have been given to pathologist, we enter the information into our LIS (Cerner Classic). > > While we would love to be able to afford to purchase one of the many positive patient ID barcode systems, this isn't feasible at this time. So I am wondering what other labs are doing to comply with positive patient ID? > > > > Thanks for all your help! > > Kelly > > > _________________________________________________________________ > Bing? brings you maps, menus, and reviews organized in one place. Try it now. > http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Lauren found her dream laptop. Find the PC that?s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290 From arvidsonkristen <@t> yahoo.com Tue Jun 23 12:41:37 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Jun 23 12:41:41 2009 Subject: [Histonet] CAP Checklists Message-ID: <617681.92513.qm@web65712.mail.ac4.yahoo.com> Would anyone be willing to share their histo CAP inspection checklist?? They want 300 to purchase one. From lblazek <@t> digestivespecialists.com Tue Jun 23 12:44:42 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jun 23 12:43:14 2009 Subject: [Histonet] Positive Patient ID In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39088C7B30EA@IBMB7Exchange.digestivespecialists.com> Kelly, Maybe the problem is in the procedure manual terminology. Do you have it spelled out that when you receive the specimen it is matched to the requisition to assure positive id? Do you have in the grossing process procedure that the specimen is matched to the cassette? And then the cassette to the slide in the sectioning step? Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Colpitts Sent: Tuesday, June 23, 2009 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Positive Patient ID Hi Histoland, Recently we had a CAP inspection. One of the deficiencies that we were sited for was a lack of positive patient ID. Here's is our current process (the process that has been in place forever and a day). 1)Specimens are collected. 2)They are assigned the next specimen numbers (the next available number is recorded on a steno pad next to the cassette labeler as well as stored in the cassette labeler). 3)Cassettes are made and the specimen, cassettes and a copy of the paper requisition are given to the pathologist to be grossed in. 4)Once all specimens have assigned numbers and have been given to pathologist, we enter the information into our LIS (Cerner Classic). While we would love to be able to afford to purchase one of the many positive patient ID barcode systems, this isn't feasible at this time. So I am wondering what other labs are doing to comply with positive patient ID? Thanks for all your help! Kelly _________________________________________________________________ Bing(tm) brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shakun.Aswani <@t> acologix.com Tue Jun 23 13:34:48 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Tue Jun 23 13:34:55 2009 Subject: [Histonet] (no subject) Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301B97BEA@EXCHANGE.acologix.com> Hi everyone, Has any one used Xylenol orange fluorochrome label for bone. If yes, please contact me directly. I need help. Thank you in advance Shakun Shakun P. Aswani Scientist I, Preclinical Development Acologix, Inc. 3960 Point Eden Way Hayward CA 94545 (510) 512-7231 phone (510) 786-1116 facsimile shakun.aswani@acologix.com From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jun 23 14:23:41 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jun 23 14:23:53 2009 Subject: [Histonet] tissue processor In-Reply-To: <37A1F9CAB9E21C41B39F3653B620D13E093BA3DE@exchange2003.lc.local> Message-ID: Hi Carol I have attached a protocol that I have used in the lab before. The protocol is for small pieces. You can add 10 minutes on to the protocol for bigger pieces and add pressure and vacuum. If you have any questions please feel free to call me. Best Regards (See attached file: GI protocol xyl ASP.doc) Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Carol Bryant" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] tissue processor 06/23/2009 09:26 AM If you were going to purchase a new tissue processor, which would you recommend for a small lab that runs ? 200 blocks per day? ?The majority of our workload is biopsies (skins & GI) with a few breast masses. ?We also do a fair volume of IHC and are reluctant to go to microwave processing for this reason. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by the State of Kentucky and/or Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient and have received this communication in error, please contact the sender and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From dellav <@t> musc.edu Tue Jun 23 15:01:48 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Jun 23 15:01:52 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: <617681.92513.qm@web65712.mail.ac4.yahoo.com> References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> Message-ID: CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 From JWeems <@t> sjha.org Tue Jun 23 15:11:32 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jun 23 15:11:36 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA56243A7@ITSSSXM01V6.one.ads.che.org> We track from time of receipt. One way you could track from time of order is if the specimen is ordered electronically. We have that set up for some of our units - a requisition is ordered with time of order, etc. But we couldn't get it working in the OR - not enough people and printers at ready access.. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, June 23, 2009 16:02 To: histonet Subject: [Histonet] tracking turnaround time of intraoperative consultations CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Maria.Katleba <@t> stjoe.org Tue Jun 23 16:16:10 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Jun 23 16:16:19 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA56243A7@ITSSSXM01V6.one.ads.che.org> References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> <5D64396A0D4A5346BEBC759022AAEAA56243A7@ITSSSXM01V6.one.ads.che.org> Message-ID: <97C02552ECB11346877D3E83CF833ABD13CEDEA9EC@SJSNT-SCMAIL03.stjoe.org> This is why we start the time when we actually receive the specimen in hand. Maria -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, June 23, 2009 1:12 PM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations We track from time of receipt. One way you could track from time of order is if the specimen is ordered electronically. We have that set up for some of our units - a requisition is ordered with time of order, etc. But we couldn't get it working in the OR - not enough people and printers at ready access.. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, June 23, 2009 16:02 To: histonet Subject: [Histonet] tracking turnaround time of intraoperative consultations CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From becky.garrison <@t> jax.ufl.edu Tue Jun 23 16:51:52 2009 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Tue Jun 23 16:51:56 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> Message-ID: We have just started tracking from order to sign out for frozen sections. (In addition, frozens are tracked from receipt in pathology to sign out using the CAP guidelines). The trouble with the electronic order (in our institution) is that the OR may place the pathology order in hospital computer system early in the surgery so that the order time that prints on the requisition is substantially different than the actual collect time. We have resolved this by having the OR staff write the actual collect time on the requisition and initial it. This collect time is also noted in the OR documentation notes for the surgery. When OR forgets to note collect time manually on the requisition (and they do), I call back and have them look up and verify the collect time. This was started with the cooperation and support of the OR administration. For the pathology accession staff, this means they can not use the order time that crosses the interface to the LIS (lab computer system) but must enter the handwritten time as noted on the requisition. We have set a goal of 40 minutes from frozen order to sign out. This may be lowered to 30 - 35 minutes depending on how our data looks over several months. Our pathology dept. is located on the first floor and the OR on second floor of same building. As for noting collect times for multiple specimens, same case: We have always required the OR to generate a requisition for each container. The collect time is written on each requisition. This is no different than writing the collect date/time and initials that nursing/phlebotomy does for each tube of blood drawn hospitalwide. Would be interested in hearing from others on how this is handled. Becky Garrison Pathology supervisor Shands Jacksonville Jacksonville, FL 32209 904-24-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, June 23, 2009 4:02 PM To: histonet Subject: [Histonet] tracking turnaround time of intraoperative consultations CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Jun 23 17:22:59 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Jun 23 17:24:31 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> , Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D362672BE@LRGHEXVS1.practice.lrgh.org> This is basically how we have always done. On scheduled frozens we have 15 minutes for turnaround, unscheduled 30 minutes. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky [becky.garrison@jax.ufl.edu] Sent: Tuesday, June 23, 2009 5:51 PM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations We have just started tracking from order to sign out for frozen sections. (In addition, frozens are tracked from receipt in pathology to sign out using the CAP guidelines). The trouble with the electronic order (in our institution) is that the OR may place the pathology order in hospital computer system early in the surgery so that the order time that prints on the requisition is substantially different than the actual collect time. We have resolved this by having the OR staff write the actual collect time on the requisition and initial it. This collect time is also noted in the OR documentation notes for the surgery. When OR forgets to note collect time manually on the requisition (and they do), I call back and have them look up and verify the collect time. This was started with the cooperation and support of the OR administration. For the pathology accession staff, this means they can not use the order time that crosses the interface to the LIS (lab computer system) but must enter the handwritten time as noted on the requisition. We have set a goal of 40 minutes from frozen order to sign out. This may be lowered to 30 - 35 minutes depending on how our data looks over several months. Our pathology dept. is located on the first floor and the OR on second floor of same building. As for noting collect times for multiple specimens, same case: We have always required the OR to generate a requisition for each container. The collect time is written on each requisition. This is no different than writing the collect date/time and initials that nursing/phlebotomy does for each tube of blood drawn hospitalwide. Would be interested in hearing from others on how this is handled. Becky Garrison Pathology supervisor Shands Jacksonville Jacksonville, FL 32209 904-24-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, June 23, 2009 4:02 PM To: histonet Subject: [Histonet] tracking turnaround time of intraoperative consultations CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Debra.Ortiz <@t> uchospitals.edu Tue Jun 23 18:04:07 2009 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Tue Jun 23 18:04:22 2009 Subject: [Histonet] GI biopsies fixed in Hollandes fixative Message-ID: <5392DB699B157E4C8E65B3779634BD7D011AF5DD@uchmbx04-hpk03s.UCHAD.uchospitals.edu> We have an on going discussion about fixation time, and rinsing time when it comes to GI bx's fixed in Hollandes. The biopsies are currently brought into the grossing room in Hollandes, and grossed in the afternoon. At that time, they are placed in 70% alcohol until the evening when they are then put on VIPS. Our questions are these: What is the general rule about how long small GI biopsies have to fix in Hollandes? How long must they rinse in 70% alcohol before going on the processors. If we are rinsing out the picric acid, can it follow the same rule as those fixed in Bouins and rinsed for approximately 30-45 minutes? Thank you very much for any information on this, Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From JMacDonald <@t> mtsac.edu Wed Jun 24 00:21:19 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jun 24 00:21:23 2009 Subject: [Histonet] 2010 CSH Symposium Message-ID: Jan, Would you be interested in giving a couple of workshops for our 2010 symposium? We will be at the Mission Inn in Riverside, CA. We have members that are interested in a frozen section workshop. Thank you, Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From JMacDonald <@t> mtsac.edu Wed Jun 24 00:23:18 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jun 24 00:23:22 2009 Subject: [Histonet] CSH 2010 Message-ID: Jan, I forgot to give you the dates. May 14, 15, 16, 2010. Jennifer From JMacDonald <@t> mtsac.edu Wed Jun 24 00:35:36 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jun 24 00:35:40 2009 Subject: [Histonet] Sorry!! Message-ID: My previous post was not meant to go to the Histonet. Jennifer From dellav <@t> musc.edu Wed Jun 24 07:43:53 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Jun 24 07:43:57 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D362672BE@LRGHEXVS1.practice.lrgh.org> References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> , <38667E7FB77ECD4E91BFAEB8D98638631D362672BE@LRGHEXVS1.practice.lrgh.org> Message-ID: Tom, are the times you've listed benchmarks your facility has established for it's operation? I'm not aware of any national benchmarks using those numbers but want to be sure I haven't missed something. Also, where is your frozen lab located in relation to the OR? Are you located within the OR area or elsewhere? And does your 15 minute benchmark include pre-analytical specimen transport time? Sorry for all of the questions, just want to be sure we are all comparing similar circumstances. thanks Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, June 23, 2009 6:23 PM To: Garrison, Becky; Della Speranza, Vinnie; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations This is basically how we have always done. On scheduled frozens we have 15 minutes for turnaround, unscheduled 30 minutes. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky [becky.garrison@jax.ufl.edu] Sent: Tuesday, June 23, 2009 5:51 PM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations We have just started tracking from order to sign out for frozen sections. (In addition, frozens are tracked from receipt in pathology to sign out using the CAP guidelines). The trouble with the electronic order (in our institution) is that the OR may place the pathology order in hospital computer system early in the surgery so that the order time that prints on the requisition is substantially different than the actual collect time. We have resolved this by having the OR staff write the actual collect time on the requisition and initial it. This collect time is also noted in the OR documentation notes for the surgery. When OR forgets to note collect time manually on the requisition (and they do), I call back and have them look up and verify the collect time. This was started with the cooperation and support of the OR administration. For the pathology accession staff, this means they can not use the order time that crosses the interface to the LIS (lab computer system) but must enter the handwritten time as noted on the requisition. We have set a goal of 40 minutes from frozen order to sign out. This may be lowered to 30 - 35 minutes depending on how our data looks over several months. Our pathology dept. is located on the first floor and the OR on second floor of same building. As for noting collect times for multiple specimens, same case: We have always required the OR to generate a requisition for each container. The collect time is written on each requisition. This is no different than writing the collect date/time and initials that nursing/phlebotomy does for each tube of blood drawn hospitalwide. Would be interested in hearing from others on how this is handled. Becky Garrison Pathology supervisor Shands Jacksonville Jacksonville, FL 32209 904-24-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, June 23, 2009 4:02 PM To: histonet Subject: [Histonet] tracking turnaround time of intraoperative consultations CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From af46 <@t> buffalo.edu Wed Jun 24 07:52:12 2009 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Wed Jun 24 07:52:17 2009 Subject: [Histonet] Sheep Lung Message-ID: We will be cutting frozen sections on sheep lung and I was wondering if anyone has a method to produce good quality sections. Are you injecting anything in the lung prior to sectioning? Annette Featherstone From alyssa <@t> alliedsearchpartners.com Wed Jun 24 08:40:07 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Jun 24 08:40:11 2009 Subject: [Histonet] Full Time Position In Florida-Near Tampa/Lakeland, Florida (Dover, FL) Message-ID: Top Recruiter For Laboratory/Pathology Professionals Allied Search Partners is a medical laboratory/pathology recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the premier permanent placement/direct hire solution for medical laboratory/pathology professionals, ASP has the following histology opening at a laboratory *in between Tampa and Lakeland, FL.* *The Dover, FL area* Day shift - 4am or 5am start time, full time ASCP required, FL license required Full time permanent position/Fully Benefited Private Laboratory setting Salary depends on experience *Please send resume for prescreening consideration to: Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From relia1 <@t> earthlink.net Wed Jun 24 09:21:29 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jun 24 09:21:34 2009 Subject: [Histonet] RELIA Histology Job Alert 6/24/2009 A few jobs to tell you about and a note about social networking Message-ID: Hi Histonetters! I hope everybody is having a great week. I have a few jobs I want to tell you about. All of these jobs are full time permanent positions with some of the finest facilities in the country. My clients offer excellent compensation, benefits and relocation assistance or sign on bonuses. Here are the jobs I am currently working on: Lead Histotechnologist - North Shore of Boston Histotechnologist - Upstate New York Night Shift Histotech - New York City Histotechnologist - Gulf Coast of Florida Histotechnician - Austin, TX Histotech and Supervisor positions - Los Angeles, CA If you or anyone you know is interested in the details on any of these positions please contact me. I can be reached by e-mail at relia1@earthlink.net or toll free at 866-607-3542. Are you into social networking? Are you on Facebook, Linkedin, Myspace or Twitter? If you are using any of these sites I would like to invite you to connect with me. On linkedin.com you can find me at: http://www.linkedin.com/in/reliasolutions On facebook.com you can find me at www.facebook.com and search Pam Barker RELIA On Myspace.com you can find me at www.myspace.com/pamatrelia On Twitter.com you can find me at www.twitter.com/pamatrelia If you are interested in joining one of these social networks and don't know how or need help please let me know. Or have questions about the who what why or where of any or all of these sites I would be happy to help you. Have a great day!! Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Aubrey <@t> nsh.org Wed Jun 24 09:46:01 2009 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Wed Jun 24 09:46:09 2009 Subject: [Histonet] NSH IHC Forum Message-ID: Registration for the First Annual One Day Immunohistochemistry Forum for Histotechs is now open. The event developed with Dr. Richard Cartun and other experts in the field is scheduled for July 18, 2009 in Indianapolis. The event will cover basic and advanced topics in the field of immunohistochemistry including sessions on ER/PR/HER2 detection, applications of IHC to cytology specimens, Kappa and Lambda detection, and emerging technologies. Thanks to the educational partnership with Ventana Medical Systems NSH is able to offer this one day forum worth 6 contact hours for the very low price of $99.00 to NSH members and $149 for non members. We hope you will be able to join us. You can find all relevant details & registration information online: https://www.nshonline.org/eweb/DynamicPage.aspx?webcode=EventInfo&Reg_ev t_key=ca4810b6-2e6a-4670-b720-d2c9d3976a47&RegPath=EventRegFees If you have any questions about the program or registering please feel free to contact the NSH Office, 443-535-4060. From ktuttle <@t> umm.edu Wed Jun 24 09:45:53 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Jun 24 09:46:40 2009 Subject: [Histonet] Budget Microarray - Finally In-Reply-To: <4A40EF24.9050501@pathology.washington.edu> References: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7@PHSXMB30.partners.org> <4A40EF24.9050501@pathology.washington.edu> Message-ID: <4A4203E1.90CE.001A.3@umm.edu> There is also a histonetter that makes silicone array molds, you can buy them online. http://www.arraymold.com/ Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> Victor Tobias 6/23/2009 11:05 am >>> Bill, Very nice and thanks for sharing. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. O'Donnell, Bill wrote: > > Good day Histonetters, > > A few weeks back, I posted that I had a method for making a tissue > microarray on the cheap. I have received a lot of requests for how I do > this. I wanted to write it up because, even though it is pretty easy to > do, it is difficult to describe. > > So...I made a PowerPoint on how to do it and posted it at this address: > > http://highperformancehistology.yolasite.com/ > > With your indulgence I will re-post a few times iin the next couple of > weeks so that those who contacted me won't miss out. (I suppose I could > have saved all those addresses...ah, the wonder of hindsight! > > William (Bill) O'Donnell, HT (ASCP) QIHC > Lead Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From jshelley <@t> burnham.org Wed Jun 24 11:34:30 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Wed Jun 24 11:34:38 2009 Subject: [Histonet] Glycine Message-ID: Hi Histonetters, I was given 3 antibodies that were worked up previously with 1% glycine for 30 mins. prior to antibody incubation. I have not come across this before and was wondering if anyone knows the purpose of its use. Is it a blocking reagent. The antibodies that I am using it on are GRP78,GRP94,PDI. Thanks for whatever insight one can share on this. John From algranth <@t> email.arizona.edu Wed Jun 24 11:36:36 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Jun 24 11:36:43 2009 Subject: [Histonet] Sevier-Munger question for special stain experts Message-ID: <8C24BEDE-3812-433B-9C81-051F56A979B2@email.arizona.edu> Good morning! If there is anyone out there who does silver stains in large batches I need your input. I haven't done a Sevier-Munger stain in probably 20 years and when I did this kind of stain I usually had 2-3 slides to stain. So now I have a request to do this stain and I have multiple racks of slides. What is the best way to handle the ammoniacal silver step - each slide individually or plunk a whole rack of slides into a staining dish? And if I do the latter, can I multiply the amounts by 5 to make 250 ml. of the ammoniacal silver solution which is the amount needed to fill the staining dish? Thanks. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From rjbuesa <@t> yahoo.com Wed Jun 24 11:43:17 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 24 11:43:21 2009 Subject: [Histonet] Sevier-Munger question for special stain experts Message-ID: <609160.88296.qm@web65712.mail.ac4.yahoo.com> I always tried to handle slides to be stained with silver individually, but if you decide to go for the whole batch, yes you can multiply the components, any way the "end point" of the solution will tell you when it is correct. Ren? J. --- On Wed, 6/24/09, Andrea Grantham wrote: From: Andrea Grantham Subject: [Histonet] Sevier-Munger question for special stain experts To: "HISTONET" Date: Wednesday, June 24, 2009, 12:36 PM Good morning! If there is anyone out there who does silver stains in large batches I need your input. I haven't done a Sevier-Munger stain in probably 20 years and when I did this kind of stain I usually had 2-3 slides to stain. So now I have a request to do this stain and I have multiple racks of slides. What is the best way to handle the ammoniacal silver step - each slide individually or plunk a whole rack of slides into a staining dish? And if I do the latter, can I multiply the amounts by 5 to make 250 ml. of the ammoniacal silver solution which is the amount needed to fill the staining dish? Thanks. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ???Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Jun 24 11:44:54 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Jun 24 11:49:37 2009 Subject: [Histonet] tracking turnaround time of intraoperative consultations In-Reply-To: References: <617681.92513.qm@web65712.mail.ac4.yahoo.com> , <38667E7FB77ECD4E91BFAEB8D98638631D362672BE@LRGHEXVS1.practice.lrgh.org>, Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D362672C0@LRGHEXVS1.practice.lrgh.org> The benchmark is for our facility, the OR is on the 3rd floor and we are on the lobby/basement. The 15 minutes are from time arrival in the lab to the Or room called. All surgical specimens are time stamped upon arrival in the lab, the only thing we do extra with frozens is to time stamp the finish time of the frozen (the time, the pathologist picks up the phone to call the OR. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: Della Speranza, Vinnie [dellav@musc.edu] Sent: Wednesday, June 24, 2009 8:43 AM To: Podawiltz, Thomas; Garrison, Becky; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations Tom, are the times you've listed benchmarks your facility has established for it's operation? I'm not aware of any national benchmarks using those numbers but want to be sure I haven't missed something. Also, where is your frozen lab located in relation to the OR? Are you located within the OR area or elsewhere? And does your 15 minute benchmark include pre-analytical specimen transport time? Sorry for all of the questions, just want to be sure we are all comparing similar circumstances. thanks Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, June 23, 2009 6:23 PM To: Garrison, Becky; Della Speranza, Vinnie; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations This is basically how we have always done. On scheduled frozens we have 15 minutes for turnaround, unscheduled 30 minutes. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky [becky.garrison@jax.ufl.edu] Sent: Tuesday, June 23, 2009 5:51 PM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations We have just started tracking from order to sign out for frozen sections. (In addition, frozens are tracked from receipt in pathology to sign out using the CAP guidelines). The trouble with the electronic order (in our institution) is that the OR may place the pathology order in hospital computer system early in the surgery so that the order time that prints on the requisition is substantially different than the actual collect time. We have resolved this by having the OR staff write the actual collect time on the requisition and initial it. This collect time is also noted in the OR documentation notes for the surgery. When OR forgets to note collect time manually on the requisition (and they do), I call back and have them look up and verify the collect time. This was started with the cooperation and support of the OR administration. For the pathology accession staff, this means they can not use the order time that crosses the interface to the LIS (lab computer system) but must enter the handwritten time as noted on the requisition. We have set a goal of 40 minutes from frozen order to sign out. This may be lowered to 30 - 35 minutes depending on how our data looks over several months. Our pathology dept. is located on the first floor and the OR on second floor of same building. As for noting collect times for multiple specimens, same case: We have always required the OR to generate a requisition for each container. The collect time is written on each requisition. This is no different than writing the collect date/time and initials that nursing/phlebotomy does for each tube of blood drawn hospitalwide. Would be interested in hearing from others on how this is handled. Becky Garrison Pathology supervisor Shands Jacksonville Jacksonville, FL 32209 904-24-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, June 23, 2009 4:02 PM To: histonet Subject: [Histonet] tracking turnaround time of intraoperative consultations CAP utilizes the term "intraoperative consultation" to describe the utilization of frozen (cryo) sections to provide a rapid diagnosis back to a surgeon in the operating room. The CAP checklist requires a turnaround time of 20 minutes for single specimen submitted for intraoperative consultation. My understanding is that the turnaround time is measured from the time the sample is received in the laboratory until the time the report is issued to the surgeon. Is anyone tracking or measuring turnaround time from the time the consult is "ordered" in/by the Operating Room until the time the result is issued? If so, would you share how you are able to determine the time the "test was ordered" and to what extent you have elicited the cooperation of Operating Room personnel. We receive many complex surgical cases and our intraoperative consults frequently consist of multiple surgical samples from the same patient arriving in the lab at the same time. Our head and neck cases, for example, consist of 6-8 biopsies that are sent to pathology at the same time. In this example, we have no knowledge of which biopsies was excised first or last and because the surgeon chooses to allow multiple samples to accumulate before sending them all off to the lab, it's clear that the true "pre-analytical" time will not be the same for each sample. If you are tracking turnaround from the time of order to the time of result reporting, how are you determining what is an acceptable turnaround time? CAP's standard is the only national standard I am aware of for frozen section turnaround times. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rsrichmond <@t> gmail.com Wed Jun 24 12:28:59 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jun 24 12:29:03 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations Message-ID: Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical University of South Carolina Charleston SC asks about tracking turnaround time of frozen sections (note that not every intraoperative consultation requires a frozen section). The few services I've worked on that attempted to track turnaround time timed them from time of receipt in the laboratory (using a time stamp for that) to telephoning the report (the pathologist had to write down the time on the hand-scribbled report). The prescribed maximum turnaround was 20 minutes, which is pretty easy to meet. Cases with multiple frozen sections were not timed. Has there been some change in the CAP requirements for recording turnaround time of frozen sections in the last three years? Bob Richmond Samurai Pathologist Knoxville TN From af46 <@t> buffalo.edu Wed Jun 24 12:53:33 2009 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Wed Jun 24 12:53:38 2009 Subject: [Histonet] MCP-1 Message-ID: Is anyone working with MCP-1 Armenian hamster, from Biolegend? This is for FFPE tissue. The secondary is anti-Syrian hamster. Any help would be appreciated. Annette Featherstone From gayle.callis <@t> bresnan.net Wed Jun 24 13:11:38 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Jun 24 13:12:09 2009 Subject: [Histonet] RE: Silver stains in large batches Message-ID: <000901c9f4f7$3c4e8c90$b4eba5b0$@callis@bresnan.net> You wrote: If there is anyone out there who does silver stains in large batches I need your input. I haven't done a Sevier-Munger stain in probably 20 years and when I did this kind of stain I usually had 2-3 slides to stain. So now I have a request to do this stain and I have multiple racks of slides. What is the best way to handle the ammoniacal silver step - each slide individually or plunk a whole rack of slides into a staining dish? And if I do the latter, can I multiply the amounts by 5 to make 250 ml. of the ammoniacal silver solution which is the amount needed to fill the staining dish? Thanks Andi ********************** Andi, I did Steiner and Steiner Helicobacter staining in huge batches, 20 slides per rack to control the timing better. I used glass staining dishes (metal is unacceptable for silver stains) and crisscrossed the slides so they were not back to back. Criss crossing slides avoids trapped reagents that cause nasty carryover. I had a positive control in EACH rack to monitor the silver deposition for that rack of slides. I monitored with a microscope and you may need to do this too. It took a bit of careful planning and with heated solutions for Steiner another dimension of difficulty. I locked doors and didn't answer telephones. The staining worked beautifully, and I could do 60 or more slides a day. Mass production is possible and if it was me doing this, there is no way I would do 2 to 3 slides at a time. It would be a nightmare workday, maybe an "all nighter" in the lab! Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 From koellingr <@t> comcast.net Wed Jun 24 13:14:01 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jun 24 13:14:03 2009 Subject: [Histonet] MCP-1 In-Reply-To: Message-ID: <1183845356.6118761245867241509.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> The hamster isotypes are tricky and ill-defined. Some people (and me formerly) use secondary cocktails. Sometimes Armenian and Syrian are interchangeable. Sometimes they are completely not. BD biosciences has a great Hamster Ig chart that you can use for flow (or IHC) reference. Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Annette Featherstone" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 24, 2009 10:53:33 AM GMT -08:00 US/Canada Pacific Subject: [Histonet] MCP-1 Is anyone working with MCP-1 Armenian hamster, from Biolegend? This is for FFPE tissue. The secondary is anti-Syrian hamster. Any help would be appreciated. Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drvet_anjan <@t> hotmail.com Wed Jun 24 13:25:33 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Wed Jun 24 13:25:36 2009 Subject: [Histonet] DAB substrate buffer Message-ID: hi every one, I am using Labvision HRP polymer secondary antibody with DAB chromogen, funny thing is that i have exhausted the DAB substrate buffer. so i tried with PBS 7.4 and h2o2 and chromogen and i found out that nothing worked. can anyone tell me a protocol or recipe for preparing DAB substrate buffer. Regards, Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ Live Search extreme As India feels the heat of poll season, get all the info you need on the MSN News Aggregator http://news.in.msn.com/National/indiaelections2009/aggregator/default.aspx From tifei <@t> foxmail.com Wed Jun 24 13:30:45 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Jun 24 13:31:02 2009 Subject: [Histonet] Silver staining /other methods to reveal axon sprouting/retraction following injury Message-ID: <200906250230405280327@foxmail.com> Hi all i read such figures in Cajal's books..I guess he is not using specific degenerative sliver staining, as Natua developed the method 50 years later. I wonder anyone can send me the protocol for such kind of silver staining - there are so many ways of Golgi staining - to reveal post-injury changes of axons after transection, for example? Also, any other comments on molecular approaches are welcome. 2009-06-25 TF From gayle.callis <@t> bresnan.net Wed Jun 24 13:42:56 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Jun 24 13:43:22 2009 Subject: [Histonet] MCP-1 In-Reply-To: <1183845356.6118761245867241509.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <1183845356.6118761245867241509.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <001301c9f4fb$9b57a1f0$d206e5d0$@callis@bresnan.net> We never mixed Armenian Hamster and Golden Syrian. Do not use the antiGolden Syrian secondary since the Armenian hamster is a different (and difficult!) little critter than Golden Syrian (I learned this from the head scientist at Jackson Immunoresearch). If you need an anti-Armenian hamster secondary antibody, buy them from Jackson ImmunoResearch. I ran into some major trouble trying to use an antiGolden Syrian secondary with an Armenian hamster monoclonal primary and also had the wrong host IgG (hamster was the word that led to confusion in my early days of IHC). When the anti-Armenian hamster secondary was used, the staining was excellent. You also need to use an Armenian hamster isotype control - these are available from BD Biosciences, do not use Golden Syrian IgG as the negative control. We frequently buy our Armenian hamster monoclonal primaries conjugated to biotin but you have to use biotinylated Armenian hamster isotype negative control. This eliminates a secondary altogether and we have excellent staining results, both IHC or IFA. Ray is correct there may be or not be cross reaction between Armenian and Golden Syrian but with antiArmenian Hamster secondaries available, you can eliminate Golden Syrian from the mix entirely. Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, June 24, 2009 12:14 PM To: Annette Featherstone Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MCP-1 The hamster isotypes are tricky and ill-defined. Some people (and me formerly) use secondary cocktails. Sometimes Armenian and Syrian are interchangeable. Sometimes they are completely not. BD biosciences has a great Hamster Ig chart that you can use for flow (or IHC) reference. Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Annette Featherstone" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 24, 2009 10:53:33 AM GMT -08:00 US/Canada Pacific Subject: [Histonet] MCP-1 Is anyone working with MCP-1 Armenian hamster, from Biolegend? This is for FFPE tissue. The secondary is anti-Syrian hamster. Any help would be appreciated. Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Jun 24 13:49:13 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Jun 24 13:49:59 2009 Subject: [Histonet] Beta-Amyloid Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A589035705B4@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi, Wondering if anyone knows of a Beta-Amyloid antibody, for use on FFPE brain sections, that doesn't need formic acid pretreatment? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From HornHV <@t> archildrens.org Wed Jun 24 14:34:14 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jun 24 14:34:22 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperativeconsultations In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D832B2@EMAIL.archildrens.org> No, it's still the same. 20 minutes is the CAP TAT. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, June 24, 2009 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: tracking turnaround time of intraoperativeconsultations Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical University of South Carolina Charleston SC asks about tracking turnaround time of frozen sections (note that not every intraoperative consultation requires a frozen section). The few services I've worked on that attempted to track turnaround time timed them from time of receipt in the laboratory (using a time stamp for that) to telephoning the report (the pathologist had to write down the time on the hand-scribbled report). The prescribed maximum turnaround was 20 minutes, which is pretty easy to meet. Cases with multiple frozen sections were not timed. Has there been some change in the CAP requirements for recording turnaround time of frozen sections in the last three years? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From dellav <@t> musc.edu Wed Jun 24 16:14:54 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Jun 24 16:14:59 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations In-Reply-To: References: Message-ID: Thanks Dr. Richmond. CAP's turnaround time requirement for frozen sections is unchanged. My question was prompted by the fact that we have an individual internal to our organization pushing for measuring turnaround from time of order to time result is issued, which muddies the water, at least for us as we do not have electronic ordering from the OR. This is prompted by JCAHO's requirement that turnaround time for critical tests be measured (Frozen section is considered a critical test by this organization) As far as I know, there is no national standard to be met if one measures turnaround from time of order, so the data then is up to the institution's interpretation for what is acceptable. One of the respondents indicated that they consider the time the sample gets to pathology as the time the test was ordered. Of those who responded to my query, one lab has electronic order entry and is just beginning to track both the in lab turnaround time and the time from order to result. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, June 24, 2009 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical University of South Carolina Charleston SC asks about tracking turnaround time of frozen sections (note that not every intraoperative consultation requires a frozen section). The few services I've worked on that attempted to track turnaround time timed them from time of receipt in the laboratory (using a time stamp for that) to telephoning the report (the pathologist had to write down the time on the hand-scribbled report). The prescribed maximum turnaround was 20 minutes, which is pretty easy to meet. Cases with multiple frozen sections were not timed. Has there been some change in the CAP requirements for recording turnaround time of frozen sections in the last three years? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Jun 24 16:19:54 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Jun 24 16:20:00 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations In-Reply-To: References: Message-ID: <4A42987A.8060407@pathology.washington.edu> Vinnie, We have always measured from the time the specimen is received in Pathology. Our frozen section room is adjacent to the OR. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Della Speranza, Vinnie wrote: > Thanks Dr. Richmond. CAP's turnaround time requirement for frozen sections is unchanged. > > My question was prompted by the fact that we have an individual internal to our organization pushing for measuring turnaround from time of order to time result is issued, which muddies the water, at least for us as we do not have electronic ordering from the OR. This is prompted by JCAHO's requirement that turnaround time for critical tests be measured (Frozen section is considered a critical test by this organization) > > As far as I know, there is no national standard to be met if one measures turnaround from time of order, so the data then is up to the institution's interpretation for what is acceptable. > > One of the respondents indicated that they consider the time the sample gets to pathology as the time the test was ordered. Of those who responded to my query, one lab has electronic order entry and is just beginning to track both the in lab turnaround time and the time from order to result. > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Tel: (843) 792-6353 > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Wednesday, June 24, 2009 1:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations > > Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical > University of South Carolina > Charleston SC asks about tracking turnaround time of frozen sections (note > that not every intraoperative consultation requires a frozen section). > > The few services I've worked on that attempted to track turnaround time > timed them from time of receipt in the laboratory (using a time stamp for > that) to telephoning the report (the pathologist had to write down the time > on the hand-scribbled report). The prescribed maximum turnaround was 20 > minutes, which is pretty easy to meet. Cases with multiple frozen sections > were not timed. > > Has there been some change in the CAP requirements for recording turnaround > time of frozen sections in the last three years? > > Bob Richmond > Samurai Pathologist > Knoxville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From alyssa <@t> alliedsearchpartners.com Wed Jun 24 16:54:48 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Jun 24 16:54:54 2009 Subject: [Histonet] Correction TO Histotech Position In FL Message-ID: Hello All, I did want to make a correction to the last email that I sent histoland. I stated in the email that the histotechnologist position in Dover, FL started at 4am, however the position actually starts earlier than that. The position starts at 2am. Please let me know if you are interested in the following: Allied Search Partners is a medical laboratory/pathology recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the premier permanent placement/direct hire solution for medical laboratory/pathology professionals, ASP has the following histology opening at a laboratory in between Tampa and Lakeland, FL. The Dover, FL area Position-Histotechnologist, experience needed Day shift - 2am start time, full time/permanent ASCP required, FL license required Full time permanent position/Fully Benefited Private Laboratory setting Salary depends on experience *Please send resume for prescreening consideration to: Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From marklove1 <@t> hotmail.com Wed Jun 24 17:33:45 2009 From: marklove1 <@t> hotmail.com (Mark Love) Date: Wed Jun 24 17:33:49 2009 Subject: [Histonet] hood for manual special stains Message-ID: The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009 From asmith <@t> mail.barry.edu Wed Jun 24 18:30:54 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jun 24 18:33:24 2009 Subject: [Histonet] hood for manual special stains In-Reply-To: References: Message-ID: I think a fume hood and a safety shower should be standard laboratory fixtures. I have a fume hood. I use it for manual coverslipping. I require my students to do the same. OSHA says that exposure to xylene fumes can cause liver damage. There is also a persistent rumor that repeated exposure to xylene fumes can cause Reynaud's disease. I handle formalin (37% formaldehyde) in the fume hood, and I would insist on having the hood if I handled full-strength formalin very often. I handle trifluoroacetic acid in the fume hood; I wouldn't use it at all without the hood. I wouldn't use ammonium sulfide without a fume hood. No one should ever handle osmium tetroxide outside of a fume hood. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Love Sent: Wednesday, June 24, 2009 6:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hood for manual special stains The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Jun 24 20:39:44 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jun 24 20:39:50 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations In-Reply-To: References: Message-ID: Vinnie, I am very interested in this discussion about tracking from time of order to completion. I am particularly interested how or why your institution decided to develop a new tracking parameter that neither CAP or JCAHO require. What drives this decision? What is the specific problem and what changes will you be able to affect once you identify the portion of the process with the most varibility? Most process improvement changes are driven by the voice of the customer. Who is the actual customer in this situation, other than the individual you mention? I would caution you to carefully consider a standard outside of the established regulatory requirements. It is very hard to "go back" to the old practice, once you have new or additional documentation and data. Without an electronic ordering system and then strict compliance to entering the actual time the specimen was removed, you are only tracking arbitrary information. Without control of the OR process, time entered into a computer, written on a requisition or verbally communicated is only as valid as the reliability of the information or the person entering the information. I do not know how we could arrive at a standard, outside of your institution or any individual institution, because there is to much variability in the pre-processing steps from excision to delivery to the lab. The parameters established in the standard set by CAP, reduces the variables and only tracks the lab processing and review time needed in the lab. Actually the standard is only a trending tool as there is only one type tracked and many exceptions. Again, I am very interested in the why's and what's associated with developing this process tracking tool. Is it quality improvement, competitive advantage or patient/surgeon request? William DeSalvo, B.S., HTL(ASCP) wdesalvo.cac@hotail.com > From: dellav@musc.edu > To: rsrichmond@gmail.com; histonet@lists.utsouthwestern.edu > Date: Wed, 24 Jun 2009 17:14:54 -0400 > Subject: RE: [Histonet] Re: tracking turnaround time of intraoperative consultations > CC: > > Thanks Dr. Richmond. CAP's turnaround time requirement for frozen sections is unchanged. > > My question was prompted by the fact that we have an individual internal to our organization pushing for measuring turnaround from time of order to time result is issued, which muddies the water, at least for us as we do not have electronic ordering from the OR. This is prompted by JCAHO's requirement that turnaround time for critical tests be measured (Frozen section is considered a critical test by this organization) > > As far as I know, there is no national standard to be met if one measures turnaround from time of order, so the data then is up to the institution's interpretation for what is acceptable. > > One of the respondents indicated that they consider the time the sample gets to pathology as the time the test was ordered. Of those who responded to my query, one lab has electronic order entry and is just beginning to track both the in lab turnaround time and the time from order to result. > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Tel: (843) 792-6353 > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Wednesday, June 24, 2009 1:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations > > Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical > University of South Carolina > Charleston SC asks about tracking turnaround time of frozen sections (note > that not every intraoperative consultation requires a frozen section). > > The few services I've worked on that attempted to track turnaround time > timed them from time of receipt in the laboratory (using a time stamp for > that) to telephoning the report (the pathologist had to write down the time > on the hand-scribbled report). The prescribed maximum turnaround was 20 > minutes, which is pretty easy to meet. Cases with multiple frozen sections > were not timed. > > Has there been some change in the CAP requirements for recording turnaround > time of frozen sections in the last three years? > > Bob Richmond > Samurai Pathologist > Knoxville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From skoost <@t> skoost.com Wed Jun 24 23:49:09 2009 From: skoost <@t> skoost.com (Mohana Gupta) Date: Wed Jun 24 23:55:46 2009 Subject: [Histonet] A little gift - Mohana Message-ID: <20090625044753.887184DC980@skoismta12.skoost.com> Mohana Gupta belongs to Skoost and sent you a little gift. Click below to collect your gift: http://www.skoost.com/fun?histonet%40lists%2Eutsouthwestern%2Eedu/20654168/5 P.S. This is a safe and innocent gift that Mohana Gupta sent from Skoost, the free goodies website. This e-mail was sent to histonet@lists.utsouthwestern.edu on 6/25/2009 5:46:29 AM on behalf of Mohana Gupta (mohana_g2002@yahoo.com) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jun 25 02:30:34 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 25 02:30:49 2009 Subject: [Histonet] hood for manual special stains Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07012733@wahtntex2.waht.swest.nhs.uk> " SKIN: Repeated contact can produce dermatitis (dryness and cracking) due to degreasing action. SKIN SENSITIZATION: Skin sensitization was not produced in any of 24 volunteers. There is one case report of a person developing an allergic skin reaction (contact urticaria) following exposure to xylene (unspecified composition) vapour. The person subsequently tested positive in a patch test. No information was provided regarding previous history of allergies. No conclusions can be drawn regarding the potential for xylene to produce allergic skin reactions, based on this single case report. NERVOUS SYSTEM EFFECTS: Long-term xylene exposure may cause harmful effects on the nervous system, but there is not enough information available to draw firm conclusions. Symptoms such as headaches, irritability, depression, insomnia, agitation, extreme tiredness, tremors, and impaired concentration and short-term memory have been reported following long-term occupational exposure to xylene and other solvents. This condition is sometimes generally referred to as "organic solvent syndrome". Unfortunately, there is very little information available which isolates xylene from other solvent exposures in the examination of these effects. Other study deficiencies include inadequate reporting on the duration of exposure and the exposure levels, and poor matching of controls. In a recent study, 175 employees were exposed to an average xylene concentration of 21 ppm for an average of 7 years. Subjective symptoms such as anxiety, forgetfulness, inability to concentrate and dizziness were reported. Xylenes accounted for greater than 70% of the total exposure.) This study is also limited by factors such those described above. BLOOD EFFECTS: Historical reports sometimes associate xylene exposure with certain blood effects, including leukemia, which are now known to be caused by benzene. Uncontaminated xylene is not known to cause these effects. Reduced blood platelet counts were observed in 12 of 27 men exposed to xylene (unspecified composition) at a level up to 200 ppm. When exposure stopped, platelet counts returned to normal. There is insufficient information to draw any conclusions from this study. LIVER AND KIDNEY EFFECTS: A number of case reports and occupational studies have suggested that liver and kidney damage may result from long-term occupational exposure to xylene. However, it is not possible to attribute these effects directly to xylene exposure because generally there was exposure to other chemicals at the same time, particularly other solvents, and there was no information provided on the exposure levels or duration of exposure. In a recent study, 175 employees were exposed to a mean xylene concentration of 21 ppm for an average of 7 years. Liver and kidney effects were not reported. Xylenes accounted for greater than 70% of the total exposure. " >From Web Site. In the UK we have to limit exposure to both formalin and xylene. Most modern Labs have downdraught dissection tables and benches have back ventilation. Neither of these chemicals ought to be tolerated if you can smell them and exposure ought to be controlled. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Love Sent: 24 June 2009 23:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hood for manual special stains The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tut orial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jun 25 02:33:14 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 25 02:33:21 2009 Subject: [Histonet] Sheep Lung Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07012734@wahtntex2.waht.swest.nhs.uk> Fixed or unfixed sheep lung? If fixed then you can perfuse with formalin from a header tank 1 metre above lung and then infiltrate with gelatine. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: 24 June 2009 13:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sheep Lung We will be cutting frozen sections on sheep lung and I was wondering if anyone has a method to produce good quality sections. Are you injecting anything in the lung prior to sectioning? Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Thu Jun 25 09:49:43 2009 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Thu Jun 25 09:49:47 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperativeconsultations In-Reply-To: References: Message-ID: Sorry I did not respond yesterday. The reason we just started measuring order to sign out for frozens was also prompted by preparation for our next JCAHO inspection. However, there is this distinction. The Joint Commission does not define a frozen as a critical test. The designation of critical test is left up to the individual institution. However, once your institution defines the frozen as a critical test, (indicated somewhere in a policy), you must conform to JCAHO guidelines for critical tests. And apparently this is the buzz with JCAHO watchers right now. Here we designate the IntraOp consultations for frozens (not gross only or Touch prep) and IntraOp PTH (Clinical test) as a critical tests and have started tracking order to sign out. Order time is the time the surgeon indicates 'send this to pathology' not when pathology receives the specimen. We are somewhat in uncharted waters as there is no national standard that defines target time from order to sign out. We set a 40 minute time (20-OR to Path and 20-path to completed frozen). I campaigned against a 30 minute total time (15 each) because we do have some frozens that do take over 15 minutes and this was an absolute value (unlike the CAP goal of 90% within 20 minutes). Our approach is to monitor, evaluate the data we retrieve. There will certainly be adjustments made to target time and how and what we monitor. The data collection raises more questions: how do you come up with meaningful data for multiple specimens on a single case; multiple frozens (different patients) received together or before we are finished with the first patient's frozen). This is one of those ideas that sounds good in theory but presents some challenges in execution. But it is a valid process to monitor as we periodically have surgeons complain of the time they are waiting for frozen results. This is really a joint quality management review which involves multiple departments (OR and Pathology) and how we make it better for the patient. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, June 24, 2009 5:15 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: tracking turnaround time of intraoperativeconsultations Thanks Dr. Richmond. CAP's turnaround time requirement for frozen sections is unchanged. My question was prompted by the fact that we have an individual internal to our organization pushing for measuring turnaround from time of order to time result is issued, which muddies the water, at least for us as we do not have electronic ordering from the OR. This is prompted by JCAHO's requirement that turnaround time for critical tests be measured (Frozen section is considered a critical test by this organization) As far as I know, there is no national standard to be met if one measures turnaround from time of order, so the data then is up to the institution's interpretation for what is acceptable. One of the respondents indicated that they consider the time the sample gets to pathology as the time the test was ordered. Of those who responded to my query, one lab has electronic order entry and is just beginning to track both the in lab turnaround time and the time from order to result. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, June 24, 2009 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical University of South Carolina Charleston SC asks about tracking turnaround time of frozen sections (note that not every intraoperative consultation requires a frozen section). The few services I've worked on that attempted to track turnaround time timed them from time of receipt in the laboratory (using a time stamp for that) to telephoning the report (the pathologist had to write down the time on the hand-scribbled report). The prescribed maximum turnaround was 20 minutes, which is pretty easy to meet. Cases with multiple frozen sections were not timed. Has there been some change in the CAP requirements for recording turnaround time of frozen sections in the last three years? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Jun 25 10:07:18 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Jun 25 10:07:27 2009 Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations In-Reply-To: References: Message-ID: <85AAE1EB-143D-49F1-B58E-21706555089C@email.arizona.edu> Some years ago (before electronic ordering systems) when I worked in the clinical lab the specimen for frozen was time stamped when it came into the lab and the pathologist stamped it - or more likely the pathologist gave it to a histotech for this - when the result was communicated to the surgeon. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Jun 24, 2009, at 2:14 PM, Della Speranza, Vinnie wrote: > Thanks Dr. Richmond. CAP's turnaround time requirement for frozen > sections is unchanged. > > My question was prompted by the fact that we have an individual > internal to our organization pushing for measuring turnaround from > time of order to time result is issued, which muddies the water, at > least for us as we do not have electronic ordering from the OR. This > is prompted by JCAHO's requirement that turnaround time for critical > tests be measured (Frozen section is considered a critical test by > this organization) > > As far as I know, there is no national standard to be met if one > measures turnaround from time of order, so the data then is up to > the institution's interpretation for what is acceptable. > > One of the respondents indicated that they consider the time the > sample gets to pathology as the time the test was ordered. Of those > who responded to my query, one lab has electronic order entry and is > just beginning to track both the in lab turnaround time and the time > from order to result. > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Tel: (843) 792-6353 > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu > ] On Behalf Of Robert Richmond > Sent: Wednesday, June 24, 2009 1:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: tracking turnaround time of intraoperative > consultations > > Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical > University of South Carolina > Charleston SC asks about tracking turnaround time of frozen sections > (note > that not every intraoperative consultation requires a frozen section). > > The few services I've worked on that attempted to track turnaround > time > timed them from time of receipt in the laboratory (using a time > stamp for > that) to telephoning the report (the pathologist had to write down > the time > on the hand-scribbled report). The prescribed maximum turnaround was > 20 > minutes, which is pretty easy to meet. Cases with multiple frozen > sections > were not timed. > > Has there been some change in the CAP requirements for recording > turnaround > time of frozen sections in the last three years? > > Bob Richmond > Samurai Pathologist > Knoxville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SDrew <@t> uwhealth.org Thu Jun 25 10:58:37 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Thu Jun 25 10:58:41 2009 Subject: [Histonet] Beta Amyloid Message-ID: <738A7878143FF74BB77436E255743C1A1F7C0F@UWHC-MAIL03.uwhis.hosp.wisc.edu> My co-worker previously asked about any versions of this antibody that didn't require formic acid pretreatment. I was wondering if there is experience out there using the antibody from DBS, and what the consensus is about the stability of the antibody (from any company) after being diluted. We currently make it up the day of use, but in anticipating an increased volume were wondering about making up larger volumes "in advance." Thank you for your thoughts and time. Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From jcox90 <@t> yahoo.com Thu Jun 25 12:52:25 2009 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu Jun 25 12:52:28 2009 Subject: [Histonet] STD testing in Lab Message-ID: <368054.11279.qm@web56805.mail.re3.yahoo.com> Hi Histonetters!!We are trying to bring in house STD testing in our lab and would like to know what type of equipment everyone uses and feedback. We would like to take on HPV, GC Chlamydia and gonorrhea. Any information would be greatly appreciated!! Thank you.. Jill Cox HT (ASCP) ? ? ? ? From nfournier <@t> sasktel.net Thu Jun 25 12:53:10 2009 From: nfournier <@t> sasktel.net (Neil M. Fournier) Date: Thu Jun 25 12:53:22 2009 Subject: [Histonet] 100 mM vs. 300 mM phosphate buffer Message-ID: <9551D614CD8848ACBBBCE882C9C436DE@Neil45EAF11E9E> Hello everyone, Is there a reason why people tend to use a phosphate buffer strength of 100 mM and not 300 mM when dissolving compounds for injections or preparing buffers for staining? Much appreciated Thanks Neil E-mail message checked by Spyware Doctor (6.0.1.441) Database version: 6.12680 http://www.pctools.com/en/spyware-doctor-antivirus/ From gvdobbin <@t> ihis.org Thu Jun 25 13:20:10 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Jun 25 13:20:31 2009 Subject: [Histonet] STD testing in Lab Message-ID: Well I would check the local human rights laws. I'm not so sure your staff are going to be too thrilled with being tested for all these diseases! .............I'M KIDDING of course. I'm sorry I can't contribute anything worthwhile (other than some comic relief) on what is otherwise a rather drab Wednesday afternoon. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Jill Cox 6/25/2009 2:52 PM >>> Hi Histonetters!!We are trying to bring in house STD testing in our lab and would like to know what type of equipment everyone uses and feedback. We would like to take on HPV, GC Chlamydia and gonorrhea. Any information would be greatly appreciated!! Thank you.. Jill Cox HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From lblazek <@t> digestivespecialists.com Thu Jun 25 13:48:12 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jun 25 13:46:41 2009 Subject: [Histonet] STD testing in Lab In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39088C7B310C@IBMB7Exchange.digestivespecialists.com> NO!!!!!! It's THURSDAY afternoon isn't it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Thursday, June 25, 2009 2:20 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STD testing in Lab Well I would check the local human rights laws. I'm not so sure your staff are going to be too thrilled with being tested for all these diseases! .............I'M KIDDING of course. I'm sorry I can't contribute anything worthwhile (other than some comic relief) on what is otherwise a rather drab Wednesday afternoon. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Jill Cox 6/25/2009 2:52 PM >>> Hi Histonetters!!We are trying to bring in house STD testing in our lab and would like to know what type of equipment everyone uses and feedback. We would like to take on HPV, GC Chlamydia and gonorrhea. Any information would be greatly appreciated!! Thank you.. Jill Cox HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Thu Jun 25 13:46:53 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Jun 25 13:47:10 2009 Subject: [Histonet] hood for manual special stains In-Reply-To: References: Message-ID: <97C02552ECB11346877D3E83CF833ABD13CF2832AB@SJSNT-SCMAIL03.stjoe.org> Why would anyone open a new lab, give cytology a hood yet have a complete disregard for histology area? I am so glad I work for Queen of the Valley Medical Center... they actually do care about ALL of there employees, no matter what department they work in. To answer your question about fumes hoods and special stains: I am sure there are many histotechs that are subjected to fumes from the special stains they do. This is because no one thinks about the consequences of us breathing heated GMS solution (heavy metal in vapor form) or phenol from the heated AFB solution you just used...or the formaldehyde (carcinogenic fumes) from the retic stain... Should I go on? This may be to norm out there, but its very very wrong and asking us histotechs to work under unsafe conditions! You must do you specials under a hood for your safety and/or be given proper PPE (personal protective equipment)! Print this e-mail and have your boss, medical director, (or whoever else thinks it's ok for an employee to be subjected to potentially harmful fumes) call me. I will be glad to talk to them about the danger of working in an unsafe condition. Have them look at the following OSHA Guidelines: Section III Chapter 3 Health Hazards/ Ventilation Section VI Chapter 1 Healthcare Facilities Section VIII Chapter 2 Personal Protective Equipment This is not much help, but maybe it will open someone's eyes to the legitimate concern you bring up. Good luck! Maria Katleba HT (ASCP), MS Pathology Dept. Manager Queen of the Valley Medical Center 707-252-4411 x3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Love Sent: Wednesday, June 24, 2009 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hood for manual special stains The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From gvdobbin <@t> ihis.org Thu Jun 25 14:04:00 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Jun 25 14:04:25 2009 Subject: [Histonet] STD testing in Lab Message-ID: Oh my gosh! You're right. My day is suddenly not so drab!! LOL Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Blazek, Linda" 6/25/2009 3:48 PM >>> NO!!!!!! It's THURSDAY afternoon isn't it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Thursday, June 25, 2009 2:20 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] STD testing in Lab Well I would check the local human rights laws. I'm not so sure your staff are going to be too thrilled with being tested for all these diseases! .............I'M KIDDING of course. I'm sorry I can't contribute anything worthwhile (other than some comic relief) on what is otherwise a rather drab Wednesday afternoon. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Jill Cox 6/25/2009 2:52 PM >>> Hi Histonetters!!We are trying to bring in house STD testing in our lab and would like to know what type of equipment everyone uses and feedback. We would like to take on HPV, GC Chlamydia and gonorrhea. Any information would be greatly appreciated!! Thank you.. Jill Cox HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From carl.hobbs <@t> kcl.ac.uk Thu Jun 25 14:25:50 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Jun 25 14:26:44 2009 Subject: [Histonet] re: Beta-amyloid Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0EFF@KCL-MAIL04.kclad.ds.kcl.ac.uk> Have a look in the image gallery here : http://www.immunoportal.com/index.php I am interested to know why you want to avoid Formic acid AR. In my XP, Formic acid exclusively exposes plaque-beta amyloid, using appropriate Abs. However, whether or not I am right, Citric acid ( or whatever your lab finds the best solution ;-) HIER also allows for non-plaque beta-Amyloid to be demonstrated. Well, it HAS to come from somewhere! lol. NB: one can do HIER followed with Formic acid, no problem. Carl From sjchtascp <@t> yahoo.com Thu Jun 25 14:44:11 2009 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Jun 25 14:44:14 2009 Subject: [Histonet] PRN work wanted in Madison, WI Message-ID: <922881.44645.qm@web38205.mail.mud.yahoo.com> I have a great LTE position as a Mohs tech at the UW .?? Need a few more hours.? Anyone know of any labs needing an as needed HT in the Madison, WI area? ? Thanks everyone, ? Steve From b-frederick <@t> northwestern.edu Thu Jun 25 15:17:23 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 25 15:17:39 2009 Subject: [Histonet] hood for manual special stains In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13CF2832AB@SJSNT-SCMAIL03.stjoe.org> Message-ID: <08EC168B5B7043B8A771960E76B88F53@lurie.northwestern.edu> All, A good source for safety issues in the histo lab is Maureen Doran. She is the safety person for the NSH. I'm sure her contact info is on the site. If not I know it is on the Illinois site (www.ilhisto.org) She is always at the NSH symposium and usually does a class, not only on safety but on zoonoses (the nasty ones) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Thursday, June 25, 2009 1:47 PM To: Mark Love; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] hood for manual special stains Why would anyone open a new lab, give cytology a hood yet have a complete disregard for histology area? I am so glad I work for Queen of the Valley Medical Center... they actually do care about ALL of there employees, no matter what department they work in. To answer your question about fumes hoods and special stains: I am sure there are many histotechs that are subjected to fumes from the special stains they do. This is because no one thinks about the consequences of us breathing heated GMS solution (heavy metal in vapor form) or phenol from the heated AFB solution you just used...or the formaldehyde (carcinogenic fumes) from the retic stain... Should I go on? This may be to norm out there, but its very very wrong and asking us histotechs to work under unsafe conditions! You must do you specials under a hood for your safety and/or be given proper PPE (personal protective equipment)! Print this e-mail and have your boss, medical director, (or whoever else thinks it's ok for an employee to be subjected to potentially harmful fumes) call me. I will be glad to talk to them about the danger of working in an unsafe condition. Have them look at the following OSHA Guidelines: Section III Chapter 3 Health Hazards/ Ventilation Section VI Chapter 1 Healthcare Facilities Section VIII Chapter 2 Personal Protective Equipment This is not much help, but maybe it will open someone's eyes to the legitimate concern you bring up. Good luck! Maria Katleba HT (ASCP), MS Pathology Dept. Manager Queen of the Valley Medical Center 707-252-4411 x3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Love Sent: Wednesday, June 24, 2009 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hood for manual special stains The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutoria l_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Jun 25 15:26:05 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jun 25 15:26:11 2009 Subject: [Histonet] Enzyme histochemistry for choline kinase Message-ID: <63EA0607835FBA4689CEA9EA8B482692020E3087@usctmx1141.merck.com> Hi all, Anyone know a way to stain tissues for choline kinase?? Thx, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Heather.D.Renko <@t> osfhealthcare.org Thu Jun 25 15:43:43 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Jun 25 15:45:44 2009 Subject: [Histonet] re: control slide storage Message-ID: Anyone have any published literature for reference on storage control; refrigerator vs. room temp vs. freezer. I have a friend asking for this and would also would love to get this information for my own reference I searched the archives but, came up with only one publication. Thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From billodonnell <@t> catholichealth.net Thu Jun 25 15:50:44 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Jun 25 15:51:03 2009 Subject: [Histonet] Budget Microarray Message-ID: Good day Histonetters, A few weeks back, I posted that I had a method for making a tissue microarray on the cheap. I have received a lot of requests for how I do this. I wanted to write it up because, even though it is pretty easy to do, it is difficult to describe. Also, keep in mind that I designed this with control blocks in mind, so, yes, they do not have 100 tissues, but I suppose it would still work with 100 if you had the patience for it. I've done as high as 30 now in a block that will fit in the red control square of the slide. So...I made a PowerPoint on how to do it (and I also posted a PDF of the PowerPoint in case of compatability issues) and posted them at this address: http://highperformancehistology.yolasite.com/ With your indulgence I will re-post a few times in the next couple of weeks so that those who contacted me won't miss out. (I suppose I could have saved all those addresses...ah, the wonder of hindsight!) William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From hfedor <@t> jhmi.edu Thu Jun 25 16:00:43 2009 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Jun 25 16:00:48 2009 Subject: [Histonet] RE: re: control slide storage In-Reply-To: References: Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D316B5FA9729@JHEMTEXVS3.win.ad.jhu.edu> Hello, We have a project that has worked on this topic using TMA's and have found that storing unstained, and unbaked slides at -20 enhances the preservation of antigenicity of the samples. It is not yet published but is getting written up. TMA slides that were stored for 5 years at -20 in very small Ziploc bags were compared to freshly cut TMA slides of the same block. Surprisingly some of the freezer slides tissue stained better then the freshly cut slides. So we need to start to store our tissue blocks in the cold as well. -- Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Thursday, June 25, 2009 4:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: control slide storage Anyone have any published literature for reference on storage control; refrigerator vs. room temp vs. freezer. I have a friend asking for this and would also would love to get this information for my own reference I searched the archives but, came up with only one publication. Thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Thu Jun 25 18:02:36 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Jun 25 18:05:34 2009 Subject: [Histonet] marilyn weiss will be out of the office Message-ID: I will be out of the office starting 06/25/2009 and will not return until 07/01/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. From tifei <@t> foxmail.com Thu Jun 25 22:07:34 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Jun 25 22:07:52 2009 Subject: [Histonet] Silver staining /other methods to reveal axonsprouting/retraction following injury Message-ID: <200906261107287802472@foxmail.com> Hi all i read such figures in Cajal's books..I guess he is not using specific degenerative sliver staining, as Natua developed the method 50 years later. I wonder anyone can send me the protocol for such kind of silver staining - there are so many ways of Golgi staining - to reveal post-injury changes of axons after transection, for example? Also, any other comments on molecular approaches are welcome. 2009-06-25 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Jun 26 06:25:49 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Jun 26 06:25:57 2009 Subject: [Histonet] Raymond Lamb BLOCKMASTER II Message-ID: <7722595275A4DD4FA225B92CDBF174A18C7DAA9AC9@EXC-MBX3.cfs.le.ac.uk> Been struggling to get the bulb out of the above inherited machine(no manual around), any ideas?, thanks. Cheers Richard Edwards LEICESTER UNIVERSITY...U.K. From tifei <@t> foxmail.com Fri Jun 26 07:43:49 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Jun 26 07:44:11 2009 Subject: [Histonet] Which kind of Golgi staining is good in visualizing AXONS Message-ID: <200906262043442148896@foxmail.com> Hi all let me sharpen my question a bit more if I want to use golgi staining to stain the axons which one should i use. i know golgi, fast golgi, golgi-cox,,,etc. 2009-06-26 TF From Maxim_71 <@t> mail.ru Fri Jun 26 08:21:33 2009 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Jun 26 08:29:46 2009 Subject: [Histonet] Please, help with VIP-processor! Message-ID: <727112987.20090626172133@mail.ru> Dear colleagues! I work at a laboratory in Taganrog, Russia with a very limited budget and a lot of work. We have an annual workload of 20,000 routine surgical cases and 850 autopsies all of which we process manually producing up to 450 blocks per day. We want to automate our processing, but we don't have money to buy a VIP-processor so I am turning to my colleagues from the net to ask: Can anyone donate a second hand VIP in working condition suitable for our daily workload? If somebody donates the VIP I will find a sponsor to pay for the shipping costs. Can anyboby help us? Please answer to me privately. Thank you and I only hope that somebody can help us! Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From akemiat3377 <@t> yahoo.com Fri Jun 26 09:28:55 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jun 26 09:28:58 2009 Subject: [Histonet] 2011 mandate for CA histology certification Message-ID: <455912.21112.qm@web31301.mail.mud.yahoo.com> Good Morning Histoland and Happy Friday! I need your assistance on a on-going question which has been kicked around since 2005.? I tried to look this up in the Histonet Archives, but was unsuccessful.? Perhaps, my key words were incorrect.? Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011.? It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants.? Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL From Robert.Lott <@t> trinitymedicalonline.com Fri Jun 26 11:00:27 2009 From: Robert.Lott <@t> trinitymedicalonline.com (Lott, Robert) Date: Fri Jun 26 11:04:29 2009 Subject: [Histonet] Frozen Section TAT Message-ID: <4A3619571D9F6C4CB79C980E91DBE4E6011560A8@TNTRIEXEVS03.triadhospitals.net> Becky Garrison makes some very valid and interesting points below.... First of all, the point about what a "critical test" is, as defined by JCHAO. She is correct, in that these are tests defined by the individual institution in policy, but not by JCAHO. Second, the item (ANP.11820) on the CAP checklist concerning turnaround time of intraoperative frozen sections reads as follows: The institution must "periodically" evaluate TAT and document reasons for delay BUT only if 90% of FS are not completed within 20 minutes. ANP.11820 Phase 1 Does the laboratory periodically evaluate turnaround time for intraoperative frozen sections? NOTE: If 90% of frozen sections are not completed within 20 minutes, the laboratory must document evaluation of the reason(s) for the delay. This turnaround time is intended to apply to the typical single frozen section. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer turnaround times may be expected. COMMENTARY: N/A REFERENCE: Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section turnaround time. A College of American Pathologists Q-Probes study of 32,868 frozen sections in 700 hospitals. Arch Pathol Lab Med. 1997;121:559-567. Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213/ 205-592-5387 Message: 19 Date: Thu, 25 Jun 2009 10:49:43 -0400 From: "Garrison, Becky" Subject: RE: [Histonet] Re: tracking turnaround time of intraoperativeconsultations To: "Della Speranza, Vinnie" , "Robert Richmond" , Message-ID: Content-Type: text/plain; charset="us-ascii" Sorry I did not respond yesterday. The reason we just started measuring order to sign out for frozens was also prompted by preparation for our next JCAHO inspection. However, there is this distinction. The Joint Commission does not define a frozen as a critical test. The designation of critical test is left up to the individual institution. However, once your institution defines the frozen as a critical test, (indicated somewhere in a policy), you must conform to JCAHO guidelines for critical tests. And apparently this is the buzz with JCAHO watchers right now. Here we designate the IntraOp consultations for frozens (not gross only or Touch prep) and IntraOp PTH (Clinical test) as a critical tests and have started tracking order to sign out. Order time is the time the surgeon indicates 'send this to pathology' not when pathology receives the specimen. We are somewhat in uncharted waters as there is no national standard that defines target time from order to sign out. We set a 40 minute time (20-OR to Path and 20-path to completed frozen). I campaigned against a 30 minute total time (15 each) because we do have some frozens that do take over 15 minutes and this was an absolute value (unlike the CAP goal of 90% within 20 minutes). Our approach is to monitor, evaluate the data we retrieve. There will certainly be adjustments made to target time and how and what we monitor. The data collection raises more questions: how do you come up with meaningful data for multiple specimens on a single case; multiple frozens (different patients) received together or before we are finished with the first patient's frozen). This is one of those ideas that sounds good in theory but presents some challenges in execution. But it is a valid process to monitor as we periodically have surgeons complain of the time they are waiting for frozen results. This is really a joint quality management review which involves multiple departments (OR and Pathology) and how we make it better for the patient. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From thisisann <@t> aol.com Fri Jun 26 11:04:01 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Jun 26 11:06:09 2009 Subject: [Histonet] Pro-Par Clearant Message-ID: <8CBC48EDB378C3D-155C-298A@mblk-d48.sysops.aol.com> Leica has informed me, yesterday,?that Pro-Par Clearant is the only Xylene Substitute that they have validated for use on the Peloris.? Is there anyone who is familiar with this clearing agent?? More importantly is anyone using it as a clearant on the Peloris tissue processor?? Leica is suggesting I use it on my small biopsy processing routine (2 hours and 9 minutes utilizing recycled alcohols as well as my larger biopsy processing routines 4 and 6 hours utilizing recycled alcohols). Thank you, Ann From lpaveli1 <@t> hurleymc.com Fri Jun 26 12:16:13 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Jun 26 12:16:35 2009 Subject: [Histonet] Pro-Par Clearant Message-ID: <4A44CA1D020000EE0002A618@smtp-gw.hurleymc.com> Hi Ann, We use ProPar on our Leica TP1050 and our Thermo Excelsior. It works very well. You must use it according to manufacturer's recommendations. We replace after 5 uses on our large specimens such as uterus, breast, etc. and after 8 uses for our small biopsies (a run of usually 20-40 specimens). All xylene substitutes are not as tolerant of water as xylene is, so for superb results, just as good as xylene, I recommend you monitor the usage/quantity of specimens. Hope this helped, Lynette >>> 06/26/09 12:04 PM >>> Leica has informed me, yesterday,?that Pro-Par Clearant is the only Xylene Substitute that they have validated for use on the Peloris.? Is there anyone who is familiar with this clearing agent?? More importantly is anyone using it as a clearant on the Peloris tissue processor?? Leica is suggesting I use it on my small biopsy processing routine (2 hours and 9 minutes utilizing recycled alcohols as well as my larger biopsy processing routines 4 and 6 hours utilizing recycled alcohols). Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From neil.macintyre <@t> btinternet.com Fri Jun 26 14:16:03 2009 From: neil.macintyre <@t> btinternet.com (neil.macintyre@btinternet.com) Date: Fri Jun 26 14:16:18 2009 Subject: [Histonet] CD34 and CD41 in paraffin sections Message-ID: <4A452C83.3560.20E3EB@neil.macintyre.btinternet.com> Hi everyone Has anyone any knowledge on the use of CD34 and CD41 in formalin fixed paraffin embedded canine tissue? It's for a suspected case of Acute Megakaryoblastic leukaemia and the identification of immature megakaryocytes. Many thanks Neil MacIntyre From ploykasek <@t> phenopath.com Fri Jun 26 14:18:30 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Jun 26 14:18:40 2009 Subject: [Histonet] DAB substrate buffer In-Reply-To: Message-ID: Hi Dr. Kumar. We use a 0.05M Tris buffer pH 7.7-7.9. We add 3% H2O2 right before use. We use our own stock DAB. Perhaps your DAB was too dilute? I hope this info helps. If you would like more specifics, you can contact me. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA > > hi every one, > I am using Labvision HRP polymer secondary antibody with DAB > chromogen, funny thing is that i have exhausted the DAB substrate buffer. so i > tried with PBS 7.4 and h2o2 and chromogen and i found out that nothing worked. > can anyone tell me a protocol or recipe for preparing DAB substrate buffer. > > Regards, > Dr. Anjan Kumar.K.R > > M.V.Sc Scholar > > Dept. of Veterinary Pathology > > Madras Veterinary College > > Chennai-7 > > India > > email: drvet_anjan@hotmail.com > > Phone: +91-9940475801 > > > > _________________________________________________________________ > Live Search extreme As India feels the heat of poll season, get all the info > you need on the MSN News Aggregator > http://news.in.msn.com/National/indiaelections2009/aggregator/default.aspx____ > ___________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From akemiat3377 <@t> yahoo.com Fri Jun 26 16:25:28 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jun 26 16:25:33 2009 Subject: Fw: RE: I'm confused [Histonet] 2011 mandate for CA histology certification Message-ID: <784766.97615.qm@web31304.mail.mud.yahoo.com> Hi Donna, I'm confused!? Are you addressing me because this was placed on the histonet? Regards, Akemi Akemi Allison-Tacha, BS, HT/HTL --- On Fri, 6/26/09, Akemi Allison-Tacha wrote: From: Akemi Allison-Tacha Subject: RE: [Histonet] 2011 mandate for CA histology certification To: "Donna Willis" Date: Friday, June 26, 2009, 2:23 PM Hi Donna, I'm confused!? Are you addressing me?? This was placed on the histonet. Regards, Akemi Allison-Tacha --- On Fri, 6/26/09, Donna Willis wrote: From: Donna Willis Subject: RE: [Histonet] 2011 mandate for CA histology certification To: "Akemi Allison-Tacha" Date: Friday, June 26, 2009, 12:27 PM Would you please post your replies to the Histonet. Thanks, Donna Willis, HT/HTL(ASCP) North American Application Manager Milestone Medical (866) 995-5300 toll free (269) 488-4040 fax www.milestonemed.com Helping Patients -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, June 26, 2009 9:29 AM To: histonet Subject: [Histonet] 2011 mandate for CA histology certification Good Morning Histoland and Happy Friday! I need your assistance on a on-going question which has been kicked around since 2005.? I tried to look this up in the Histonet Archives, but was unsuccessful.? Perhaps, my key words were incorrect.? Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011.? It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants.? Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Fri Jun 26 21:09:25 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Jun 26 21:09:28 2009 Subject: [Histonet] IHC stainer- Nemisis Message-ID: Hello Netters, Our lab is thinking about purchasing a Nemisis IHC stainer, any opinions about this stainer??? Thanks in advance. Sheila Adey HT MLT _________________________________________________________________ Attention all humans. We are your photos. Free us. http://go.microsoft.com/?linkid=9666046 From thomas.crowell <@t> novartis.com Sat Jun 27 19:26:57 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Sat Jun 27 19:27:09 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 06/27/2009 and will not return until 07/01/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. From histotechkb <@t> gmail.com Sun Jun 28 10:21:56 2009 From: histotechkb <@t> gmail.com (Kristen Yaros) Date: Sun Jun 28 10:22:02 2009 Subject: Fw: RE: I'm confused [Histonet] 2011 mandate for CA histology certification In-Reply-To: <784766.97615.qm@web31304.mail.mud.yahoo.com> References: <784766.97615.qm@web31304.mail.mud.yahoo.com> Message-ID: <667c97ab0906280821x753f5e95k4353542fc9f49515@mail.gmail.com> I believe she is asking you to share the information that you receive with everyone. Kristen On Fri, Jun 26, 2009 at 5:25 PM, Akemi Allison-Tacha wrote: > Hi Donna, > > > > I'm confused! Are you addressing me because this was placed on the > histonet? > > Regards, > Akemi > > Akemi Allison-Tacha, BS, HT/HTL > > > > > --- On Fri, 6/26/09, Akemi Allison-Tacha wrote: > > From: Akemi Allison-Tacha > Subject: RE: [Histonet] 2011 mandate for CA histology certification > To: "Donna Willis" > Date: Friday, June 26, 2009, 2:23 PM > > Hi Donna, > > > > I'm confused! Are you addressing me? This was placed on the histonet. > > Regards, > Akemi Allison-Tacha > > > > --- On Fri, 6/26/09, Donna Willis wrote: > > From: Donna Willis > Subject: RE: [Histonet] 2011 mandate for CA histology certification > To: "Akemi Allison-Tacha" > Date: Friday, June 26, 2009, 12:27 PM > > Would you please post your replies to the Histonet. > > Thanks, > > Donna Willis, HT/HTL(ASCP) > North American Application Manager > Milestone Medical > (866) 995-5300 toll free > (269) 488-4040 fax > www.milestonemed.com > > Helping Patients > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison-Tacha > Sent: Friday, June 26, 2009 9:29 AM > To: histonet > Subject: [Histonet] 2011 mandate for CA histology certification > > Good Morning Histoland and Happy Friday! > > I need your assistance on a on-going question which has been kicked around > since 2005. I tried to look this up in the Histonet Archives, but was > unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my > understanding that Governor Arnold Schwarzenegger put a mandate on all > laboratory staff, including histologists, that they were required to be > certified by 2011. It was further my understanding that un-certified > histology staff > were only going to be able to work as histology assistants. Could I get > some clarification on this matter. > > Thanks, > Akemi Allison-Tacha BS, HT/HTL > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com From Rcartun <@t> harthosp.org Sun Jun 28 19:47:27 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Jun 28 19:47:35 2009 Subject: [Histonet] Beta-Amyloid In-Reply-To: <5998F3BDFF7AAC4091C7AE93A7A1A589035705B4@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <5998F3BDFF7AAC4091C7AE93A7A1A589035705B4@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <4A47D6DF.7400.0077.1@harthosp.org> We've been using a monoclonal antibody (clone 6E10) from Signet Laboratories (now Invitrogen) for years with excellent results. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sebree Linda A" 6/24/2009 2:49 PM >>> Hi, Wondering if anyone knows of a Beta-Amyloid antibody, for use on FFPE brain sections, that doesn't need formic acid pretreatment? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.PALMER <@t> svhm.org.au Mon Jun 29 01:36:12 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Mon Jun 29 01:36:18 2009 Subject: [Histonet] Millipore MAB1281 anti human nuclei IHC on FFPE Message-ID: Hi all. Just wondering if anyone has managed to use Millipore mouse anti human nuclei antibody (clone 235-1, MAB1281) successfully with immunostaining of FFPE tissues?? The datasheet says it is possible, at 1:20 and with citrate retrieval but I have had no luck with this on a variety of human tissues, using a standard ABC with DAB protocol. Also no luck with proteinase K. (There is also an IHC world protocol which uses 1:20 with citrate.) I see some apparent labelling of epidermal nuclei, for example, maybe half of all nuclei , but also see the same thing in diluent-only negative controls, so clearly the staining seen is not specific. Thanks for any help, Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From dchihc <@t> yahoo.com Mon Jun 29 08:58:21 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Mon Jun 29 08:58:27 2009 Subject: [Histonet] Frozen Section TAT In-Reply-To: <4A3619571D9F6C4CB79C980E91DBE4E6011560A8@TNTRIEXEVS03.triadhospitals.net> References: <4A3619571D9F6C4CB79C980E91DBE4E6011560A8@TNTRIEXEVS03.triadhospitals.net> Message-ID: <327546.90788.qm@web43502.mail.sp1.yahoo.com> Hi All, ? I haven't been on in a couple of weeks, but thank all of you for the discussion on FS turnaround time reporting. I thought we had everything in place, but one thing we didn't even consider is when breast biopsies are send for radiology before?arriving for FS. ? We are also reporting receiving time as order time because they come in a pneumatic zip tube in??-60 seconds. I guess if there is more than one room trying to?send and are in line waiting to be zipped, then?the order time would be inaccurate?the way?we are reporting it. ? Thanks all!! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Lott, Robert" To: histonet@lists.utsouthwestern.edu Sent: Friday, June 26, 2009 11:00:27 AM Subject: [Histonet] Frozen Section TAT Becky Garrison makes some very valid and interesting points below.... First of all, the point about what a "critical test" is, as defined by JCHAO. She is correct, in that these are tests defined by the individual institution in policy, but not by JCAHO. Second, the item (ANP.11820) on the CAP checklist concerning turnaround time of intraoperative frozen sections reads as follows: The institution must "periodically" evaluate TAT and document reasons for delay BUT only if 90% of FS are not completed within 20 minutes. ANP.11820? ? ? ? ? ? ? ? ? ? Phase 1 Does the laboratory periodically evaluate turnaround time for intraoperative frozen sections? NOTE:? If 90% of frozen sections are not completed within 20 minutes, the laboratory must document evaluation of the reason(s) for the delay. This turnaround time is intended to apply to the typical single frozen section. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer turnaround times may be expected. COMMENTARY:? ? ? ? ? ? ? ? ? N/A REFERENCE:? Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section turnaround time. A College of American Pathologists Q-Probes study of 32,868 frozen sections in 700 hospitals. Arch Pathol Lab Med. 1997;121:559-567. Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213/ 205-592-5387 Message: 19 Date: Thu, 25 Jun 2009 10:49:43 -0400 From: "Garrison, Becky" Subject: RE: [Histonet] Re: tracking turnaround time of ? ? ? intraoperativeconsultations To: "Della Speranza, Vinnie" , "Robert Richmond" ? ? ? , Message-ID: ? ? ? Content-Type: text/plain;? ? charset="us-ascii" Sorry I did not respond yesterday.? The reason we just started measuring order to sign out for frozens was also prompted by preparation for our next JCAHO inspection.? However, there is this distinction.? The Joint Commission does not define a frozen as a critical test.? The designation of critical test is left up to the individual institution.? However, once your institution defines the frozen as a critical test, (indicated somewhere in a policy), you must conform to JCAHO guidelines for critical tests. And apparently this is the buzz with JCAHO watchers right now. Here we designate the IntraOp consultations for frozens (not gross only or Touch prep)? and IntraOp PTH (Clinical test) as a critical tests and have started tracking order to sign out.? Order time is the time the surgeon indicates 'send this to pathology' not when pathology receives the specimen. We are somewhat in uncharted waters as there is no national standard that defines target time from order to sign out.? We set a 40 minute time (20-OR to Path and 20-path to completed frozen). I campaigned against a 30 minute total time (15 each) because we do have some frozens that do take over 15 minutes and this was an absolute value (unlike the CAP goal of 90% within 20 minutes).? Our approach is to monitor, evaluate the data we retrieve.? There will certainly be adjustments made to target time and how and what we monitor.? The data collection raises more questions:? how do you come up with meaningful data for multiple specimens on a single case; multiple frozens (different patients) received together or before we are finished with the first patient's frozen).? This is one of those ideas that sounds good in theory but presents some challenges in execution. But it is a valid process to monitor as we periodically have surgeons complain of the time they are waiting for frozen results.? This is really a joint quality management review which involves multiple departments (OR and Pathology) and how we make it better for the patient. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL? 32209 904-244-6237 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thachdc <@t> niaid.nih.gov Mon Jun 29 10:31:25 2009 From: thachdc <@t> niaid.nih.gov (Thach, Dzung (NIH/NIAID) [E]) Date: Mon Jun 29 10:31:32 2009 Subject: [Histonet] Signs of good perfusion Message-ID: Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID From dchihc <@t> yahoo.com Mon Jun 29 10:47:07 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Mon Jun 29 10:47:13 2009 Subject: [Histonet] Cardboard Paraffin Catchers Message-ID: <372922.65853.qm@web43506.mail.sp1.yahoo.com> Does anyone remember the little cardboard?microtome trays for paraffin waste? If so please email me the company that makes them. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From leiker <@t> buffalo.edu Mon Jun 29 11:07:04 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Jun 29 11:07:11 2009 Subject: [Histonet] Signs of good perfusion In-Reply-To: References: Message-ID: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculature....ideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" wrote: > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out of mouth. Occasionally, I see the liver fade to light pink. Most of > the time the paws become white. But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused. I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From sjchtascp <@t> yahoo.com Mon Jun 29 11:39:02 2009 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon Jun 29 11:39:05 2009 Subject: [Histonet] WI/MI Message-ID: <385293.93112.qm@web38204.mail.mud.yahoo.com> I'm looking for a few techs from Northern WI or Northern MI to let me know when HT positions become availiable. ? Thanks From lloyd.3 <@t> osu.edu Mon Jun 29 13:11:18 2009 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Mon Jun 29 13:11:24 2009 Subject: [Histonet] Removing silver Message-ID: <04A71EFE05FE5F4DAA05D0500FD059701C8BE5@Distal.dentnet.dent.ohio-state.edu> I have a Bielchowsy stained slide and my pathologist wants to know if I can remove the silver to stain with H&E. I am not sure if that can be done. Does anyone have a suggestions? Thanks Mary L From carl.hobbs <@t> kcl.ac.uk Mon Jun 29 13:32:33 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon Jun 29 13:32:50 2009 Subject: [Histonet] Re:Beta-Amyloid Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0F13@KCL-MAIL04.kclad.ds.kcl.ac.uk> Richard.....I agree but I worry that " antibody 6E10 reacts to the abnormally processed isoforms, as well as precursor forms." ( my bold) Does this mean that this Ab also demonstrates APP? I must admit to confusion re this statement. I would appreciate your further opinion. respectfully, carl From rjbuesa <@t> yahoo.com Mon Jun 29 15:03:31 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 29 15:03:34 2009 Subject: [Histonet] Removing silver Message-ID: <589864.94479.qm@web65712.mail.ac4.yahoo.com> Apply Lugol solution and when it is totally dark brown, apply sodium tyhisulfate until it is destained. ren? J. --- On Mon, 6/29/09, Mary Lloyd wrote: From: Mary Lloyd Subject: [Histonet] Removing silver To: histonet@lists.utsouthwestern.edu Date: Monday, June 29, 2009, 2:11 PM I have a Bielchowsy stained slide and my pathologist wants to know if I can remove the silver to stain with H&E.? I am not sure if that can be done.? Does anyone have a suggestions?? Thanks Mary L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nickandmanda <@t> paradise.net.nz Mon Jun 29 15:57:45 2009 From: nickandmanda <@t> paradise.net.nz (Nick and Amanda) Date: Mon Jun 29 15:58:00 2009 Subject: [Histonet] Re: Histonet Digest, Vol 67, Issue 32 References: <7d8uc3$3vmjtj@mxin2-orange.clear.net.nz> Message-ID: <000301c9f8fc$40fc66a0$c8084979@bow1> Re Message 5 If you find pariffin catchers can I please have the details too? THANX A. Bowden amanda.bowden@ccdhb.org.nz ----- Original Message ----- From: To: Sent: Tuesday, June 30, 2009 5:02 AM Subject: Histonet Digest, Vol 67, Issue 32 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Beta-Amyloid (Richard Cartun) > 2. Millipore MAB1281 anti human nuclei IHC on FFPE > (PALMER Jason (SVHM)) > 3. Re: Frozen Section TAT (Phyllis Thaxton) > 4. Signs of good perfusion (Thach, Dzung (NIH/NIAID) [E]) > 5. Cardboard Paraffin Catchers (Phyllis Thaxton) > 6. Re: Signs of good perfusion (Merced M Leiker) > 7. WI/MI (Steven Coakley) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 28 Jun 2009 20:47:27 -0400 > From: "Richard Cartun" > Subject: Re: [Histonet] Beta-Amyloid > To: "Histonet" , "Sebree Linda A" > > Message-ID: <4A47D6DF.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > We've been using a monoclonal antibody (clone 6E10) from Signet > Laboratories (now Invitrogen) for years with excellent results. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > >>>> "Sebree Linda A" 6/24/2009 2:49 PM >>> > Hi, > Wondering if anyone knows of a Beta-Amyloid antibody, for use on FFPE > brain sections, that doesn't need formic acid pretreatment? > > Thanks, > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Mon, 29 Jun 2009 16:36:12 +1000 > From: "PALMER Jason (SVHM)" > Subject: [Histonet] Millipore MAB1281 anti human nuclei IHC on FFPE > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hi all. > > Just wondering if anyone has managed to use Millipore mouse anti human > nuclei antibody (clone 235-1, MAB1281) successfully with immunostaining of > FFPE tissues?? The datasheet says it is possible, at 1:20 and with > citrate retrieval but I have had no luck with this on a variety of human > tissues, using a standard ABC with DAB protocol. Also no luck with > proteinase K. (There is also an IHC world protocol which uses 1:20 with > citrate.) I see some apparent labelling of epidermal nuclei, for example, > maybe half of all nuclei , but also see the same thing in diluent-only > negative controls, so clearly the staining seen is not specific. > > Thanks for any help, > > Jason > > Jason Palmer > Histology Laboratory Coordinator > Bernard O'Brien Institute > 42 Fitzroy St, Fitzroy Victoria 3065 > Australia > tel +61 3 9288 4018 > fax +61 3 9416 0926 > email: jason.palmer@svhm.org.au > > Disclaimer : The contents of this e-mail including any attachments are > intended only for the person or entity to which this e-mail is addressed > and may contain confidential, privileged and/or commercially sensitive > material. If you are not, or believe you may not be, the intended > recipient, please advise the sender immediately by return e-mail, delete > this e-mail and destroy any copies. > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > > > ------------------------------ > > Message: 3 > Date: Mon, 29 Jun 2009 06:58:21 -0700 (PDT) > From: Phyllis Thaxton > Subject: Re: [Histonet] Frozen Section TAT > To: "Lott, Robert" , > histonet@lists.utsouthwestern.edu > Message-ID: <327546.90788.qm@web43502.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi All, > I haven't been on in a couple of weeks, but thank all of you for the > discussion on FS turnaround time reporting. I thought we had everything in > place, but one thing we didn't even consider is when breast biopsies are > send for radiology before arriving for FS. > We are also reporting receiving time as order time because they come in a > pneumatic zip tube in -60 seconds. I guess if there is more than one room > trying to send and are in line waiting to be zipped, then the order time > would be inaccurate the way we are reporting it. > Thanks all!! > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > ________________________________ > From: "Lott, Robert" > To: histonet@lists.utsouthwestern.edu > Sent: Friday, June 26, 2009 11:00:27 AM > Subject: [Histonet] Frozen Section TAT > > Becky Garrison makes some very valid and interesting points below.... > > > > First of all, the point about what a "critical test" is, as defined by > JCHAO. > > She is correct, in that these are tests defined by the individual > institution in policy, but not by JCAHO. > > > > Second, the item (ANP.11820) on the CAP checklist concerning turnaround > time of intraoperative frozen sections reads as follows: > > The institution must "periodically" evaluate TAT and document reasons > for delay BUT only if 90% of FS are not completed within 20 minutes. > > > > ANP.11820 Phase 1 > > > > Does the laboratory periodically evaluate turnaround time for > intraoperative frozen sections? > > > > NOTE: If 90% of frozen sections are not completed within 20 minutes, > the laboratory must document evaluation > > of the reason(s) for the delay. This turnaround time is intended to > apply to the typical single frozen section. > > In cases where there are multiple sequential frozen sections required on > a single specimen (e.g., resection margins), > > or in cases where additional studies such as radiographic correlation > are required, longer turnaround times may be expected. > > > > COMMENTARY: > > > > N/A > > > > REFERENCE: > > Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section > turnaround time. > > A College of American Pathologists Q-Probes study of 32,868 frozen > sections in 700 hospitals. > > Arch Pathol Lab Med. 1997;121:559-567. > > > > > > > > > > > > > > > > Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology > > Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL > 35213/ 205-592-5387 > > > > > > Message: 19 > > Date: Thu, 25 Jun 2009 10:49:43 -0400 > > From: "Garrison, Becky" > > Subject: RE: [Histonet] Re: tracking turnaround time of > > intraoperativeconsultations > > To: "Della Speranza, Vinnie" , "Robert Richmond" > > , > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > Sorry I did not respond yesterday. The reason we just started measuring > > order to sign out for frozens was also prompted by preparation for our > > next JCAHO inspection. However, there is this distinction. The Joint > > Commission does not define a frozen as a critical test. The designation > > of critical test is left up to the individual institution. However, > > once your institution defines the frozen as a critical test, (indicated > > somewhere in a policy), you must conform to JCAHO guidelines for > > critical tests. > > And apparently this is the buzz with JCAHO watchers right now. > > > > Here we designate the IntraOp consultations for frozens (not gross only > > or Touch prep) and IntraOp PTH (Clinical test) as a critical tests > > and have started tracking order to sign out. Order time is the time the > > surgeon indicates 'send this to pathology' not when pathology receives > > the specimen. > > > > We are somewhat in uncharted waters as there is no national standard > > that defines target time from order to sign out. We set a 40 minute > > time (20-OR to Path and 20-path to completed frozen). I campaigned > > against a 30 minute total time (15 each) because we do have some frozens > > that do take over 15 minutes and this was an absolute value (unlike the > > CAP goal of 90% within 20 minutes). Our approach is to monitor, > > evaluate the data we retrieve. There will certainly be adjustments made > > to target time and how and what we monitor. > > > > The data collection raises more questions: how do you come up with > > meaningful data for multiple specimens on a single case; multiple > > frozens (different patients) received together or before we are finished > > with the first patient's frozen). This is one of those ideas that > > sounds good in theory but presents some challenges in execution. But it > > is a valid process to monitor as we periodically have surgeons complain > > of the time they are > > waiting for frozen results. This is really a joint quality management > > review which involves multiple departments (OR and Pathology) and how we > > make it better for the patient. > > > > > > Becky Garrison > > Pathology Supervisor > > Shands Jacksonville > > Jacksonville, FL 32209 > > 904-244-6237 > > > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Mon, 29 Jun 2009 11:31:25 -0400 > From: "Thach, Dzung (NIH/NIAID) [E]" > Subject: [Histonet] Signs of good perfusion > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and > then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out > of mouth. Occasionally, I see the liver fade to light pink. Most of the > time the paws become white. But the brain and spinal cord (my tissues of > interest) are always white, and seem to have been perfused. I was > wondering > how to improve this to get more consistent good perfusions, and what signs > should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > > > ------------------------------ > > Message: 5 > Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT) > From: Phyllis Thaxton > Subject: [Histonet] Cardboard Paraffin Catchers > To: Histonet@lists.utsouthwestern.edu > Message-ID: <372922.65853.qm@web43506.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Does anyone remember the little cardboard microtome trays for paraffin > waste? If so please email me the company that makes them. > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > ------------------------------ > > Message: 6 > Date: Mon, 29 Jun 2009 12:07:04 -0400 > From: Merced M Leiker > Subject: Re: [Histonet] Signs of good perfusion > To: "Thach, Dzung (NIH/NIAID) [E]" , > histonet@lists.utsouthwestern.edu > Message-ID: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> > Content-Type: text/plain; charset=us-ascii; format=flowed > > Live going pale is a good sign, your tissues of interest going pale is an > even better sign, but fluid coming out of the mouth (or even the nose, or > additionally, any kind of bloating or swelling in the animal) is a bad > sign. You may not be able to get good perfusion (pale tissues) if this > happens before your tissues turn pale, as the pressure is too high causing > fluid to leak out of the vasculature....ideally you want to push the blood > out through the the hole you made in the right atrium, not through the > walls of the vessels. at what rate do you perfuse? if this happens a lot > slow it down. > > Hope this helps. > > --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" > wrote: > >> Hi Everyone!! >> >> I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am >> nicking the upper right atrium of heart to collect the gushed out blood >> and then perfusing through ventricle using a 21G butterfly needle and >> peristaltic pump. Sometimes I see the lungs swelling up and fluid comes >> out of mouth. Occasionally, I see the liver fade to light pink. Most of >> the time the paws become white. But the brain and spinal cord (my >> tissues of interest) are always white, and seem to have been perfused. I >> was wondering how to improve this to get more consistent good perfusions, >> and what signs should I look for to indicate good perfusion? >> >> Thanks much, >> >> Dzung >> NIAID >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > > ------------------------------ > > Message: 7 > Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT) > From: Steven Coakley > Subject: [Histonet] WI/MI > To: Histonet@lists.utsouthwestern.edu > Message-ID: <385293.93112.qm@web38204.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I'm looking for a few techs from Northern WI or Northern MI to let me know > when HT positions become availiable. > > Thanks > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 67, Issue 32 > **************************************** > From nicholasprosenbaum <@t> yahoo.com Mon Jun 29 16:02:12 2009 From: nicholasprosenbaum <@t> yahoo.com (Nicholas Rosenbaum) Date: Mon Jun 29 16:02:16 2009 Subject: [Histonet] Removing silver In-Reply-To: <589864.94479.qm@web65712.mail.ac4.yahoo.com> References: <589864.94479.qm@web65712.mail.ac4.yahoo.com> Message-ID: <787811.96420.qm@web35205.mail.mud.yahoo.com> Rene, Does this method work with all silver stains? Nick R. HT (ASCP) ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; Mary Lloyd Sent: Monday, June 29, 2009 4:03:31 PM Subject: Re: [Histonet] Removing silver Apply Lugol solution and when it is totally dark brown, apply sodium tyhisulfate until it is destained. ren? J. --- On Mon, 6/29/09, Mary Lloyd wrote: From: Mary Lloyd Subject: [Histonet] Removing silver To: histonet@lists.utsouthwestern.edu Date: Monday, June 29, 2009, 2:11 PM I have a Bielchowsy stained slide and my pathologist wants to know if I can remove the silver to stain with H&E. I am not sure if that can be done. Does anyone have a suggestions? Thanks Mary L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon Jun 29 17:43:55 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Jun 29 17:44:02 2009 Subject: [Histonet] Used equipment dealers Message-ID: Hi all. I would appreciate any info on used histology equipment dealers ( such as tissue processors) on the West Coast. Thanks for the help. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From kenp <@t> labx.com Mon Jun 29 18:07:05 2009 From: kenp <@t> labx.com (Ken Piech) Date: Mon Jun 29 18:07:48 2009 Subject: [Histonet] Used equipment dealers In-Reply-To: References: Message-ID: <0CD406B070D79E4491D59B40BE47FCA9037007F9@server1.labx.local> Hi Patti, LabX has a section specifically for used histology equipment including Tissue Processors so you could buy direct. However, if you want a company, within each category there is a directory of links to used equipment dealers for that category. http://www.labx.com/v2/category_main.cfm?MainCatID=28&CatID=354 Tissue Processor Company Links found here (and a Google map to show where companies are): http://www.labx.com/v2/lablinks/linkscategory.cfm?catID=356 Pacific Southwest Lab Equipment is one histology vendor in California: http://www.psl-equip.com Myco is in Washington: http://www.myco-instrumentation.com/ PS. You can always run a free equipment wanted ad on LabX too (no cost ever, totally free). Regards, Ken Piech General Manager LabX & Lab Manager Magazine www.labx.com www.labmanager.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Monday, June 29, 2009 6:44 PM To: histonet Subject: [Histonet] Used equipment dealers Hi all. I would appreciate any info on used histology equipment dealers ( such as tissue processors) on the West Coast. Thanks for the help. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Mon Jun 29 18:20:55 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Jun 29 18:21:04 2009 Subject: [Histonet] Used equipment dealers In-Reply-To: <0CD406B070D79E4491D59B40BE47FCA9037007F9@server1.labx.local> References: <0CD406B070D79E4491D59B40BE47FCA9037007F9@server1.labx.local> Message-ID: <97C02552ECB11346877D3E83CF833ABD13CFF84478@SJSNT-SCMAIL03.stjoe.org> Pacific Southwest Lab Equipment is one histology vendor in California: http://www.psl-equip.com Has a very nice rep, Cathy who will go out of her way for you..if she is still with them. Maria -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ken Piech Sent: Monday, June 29, 2009 4:07 PM To: histonet Subject: RE: [Histonet] Used equipment dealers Hi Patti, LabX has a section specifically for used histology equipment including Tissue Processors so you could buy direct. However, if you want a company, within each category there is a directory of links to used equipment dealers for that category. http://www.labx.com/v2/category_main.cfm?MainCatID=28&CatID=354 Tissue Processor Company Links found here (and a Google map to show where companies are): http://www.labx.com/v2/lablinks/linkscategory.cfm?catID=356 Pacific Southwest Lab Equipment is one histology vendor in California: http://www.psl-equip.com Myco is in Washington: http://www.myco-instrumentation.com/ PS. You can always run a free equipment wanted ad on LabX too (no cost ever, totally free). Regards, Ken Piech General Manager LabX & Lab Manager Magazine www.labx.com www.labmanager.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Monday, June 29, 2009 6:44 PM To: histonet Subject: [Histonet] Used equipment dealers Hi all. I would appreciate any info on used histology equipment dealers ( such as tissue processors) on the West Coast. Thanks for the help. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From saby_joseph_a <@t> yahoo.com Mon Jun 29 18:38:27 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Jun 29 18:38:30 2009 Subject: [Histonet] Signs of good perfusion In-Reply-To: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> References: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <249792.6122.qm@web33802.mail.mud.yahoo.com> I agree that the pale liver is a great sign.? However, if the lungs fill up fluid comes out the nostrels or mouth, then the needle is probably inserted too far and has gone into the pulmonary vein. I hope this helps! Joe ________________________________ From: Merced M Leiker To: "Thach, Dzung (NIH/NIAID) [E]" ; histonet@lists.utsouthwestern.edu Sent: Monday, June 29, 2009 12:07:04 PM Subject: Re: [Histonet] Signs of good perfusion Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculature....ideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" wrote: > Hi Everyone!! > >? ? I am perfusing CO2 euthanized 3 weeks old mice with PBS only.? I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump.? Sometimes I see the lungs swelling up and fluid comes > out of mouth.? Occasionally, I see the liver fade to light pink.? Most of > the time the paws become white.? But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused.? I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY? 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stephen.asquith <@t> petermac.org Mon Jun 29 20:24:03 2009 From: stephen.asquith <@t> petermac.org (Asquith Stephen) Date: Mon Jun 29 20:25:08 2009 Subject: [Histonet] Leica Autostainer XL Problems Message-ID: <6D3E01C097DB5C479B25F4063F6385CB03728678@PMC-EMAIL.petermac.org.au> Hi All, We have a Leica Autostainer XL that we do all our routine HE slides on. The section on the upper end of the slide is often unevenly stained. We have tried many things to improve the situation including; Ensuring wash tubs are flowing correctly and Increasing dips at each staining tub. If we "use" the same program but do it by hand the problem goes away. Any suggestion that may help would be most welcome. Steve Stephen Asquith Microscopy Imaging and Research Core Facility Peter MacCallum Cancer Centre Locked Bag #1 A'Beckett Street East Melbourne 8006 Email: stephen.asquith@petermac.org Phone +61-3-9656 1244/1243 Fax +61-3-9656 1411 This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. From jayaprakash.raghul <@t> rccltd.in Mon Jun 29 23:02:36 2009 From: jayaprakash.raghul <@t> rccltd.in (raghul) Date: Mon Jun 29 23:03:12 2009 Subject: [Histonet] signs of good perfusion Message-ID: <000001c9f937$9c779550$d566bff0$@raghul@rccltd.in> Dear Dzung, I wonder whether the perfusion would be good in an euthanized animal with the circulation arrested. It would be better to try it under deep anesthesia. Certainly perfusion would be better Regards Raghul RCC laboratories India Pvt. Ltd Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID ------------------------------ Message: 5 Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT) From: Phyllis Thaxton Subject: [Histonet] Cardboard Paraffin Catchers To: Histonet@lists.utsouthwestern.edu Message-ID: <372922.65853.qm@web43506.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone remember the little cardboard microtome trays for paraffin waste? If so please email me the company that makes them. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ------------------------------ Message: 6 Date: Mon, 29 Jun 2009 12:07:04 -0400 From: Merced M Leiker Subject: Re: [Histonet] Signs of good perfusion To: "Thach, Dzung (NIH/NIAID) [E]" , histonet@lists.utsouthwestern.edu Message-ID: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculature....ideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" wrote: > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out of mouth. Occasionally, I see the liver fade to light pink. Most of > the time the paws become white. But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused. I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 7 Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT) From: Steven Coakley Subject: [Histonet] WI/MI To: Histonet@lists.utsouthwestern.edu Message-ID: <385293.93112.qm@web38204.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I'm looking for a few techs from Northern WI or Northern MI to let me know when HT positions become availiable. Thanks ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 32 **************************************** From ree3 <@t> leicester.ac.uk Tue Jun 30 04:06:09 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Jun 30 04:06:15 2009 Subject: [Histonet] RE: Signs of good perfusion In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A18C7DAA9AD9@EXC-MBX3.cfs.le.ac.uk> A stiff tail!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thach, Dzung (NIH/NIAID) [E] Sent: 29 June 2009 16:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Signs of good perfusion Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Jun 30 07:15:41 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jun 30 07:15:50 2009 Subject: [Histonet] signs of good perfusion Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07012B36@wahtntex2.waht.swest.nhs.uk> That's cruel; how would you like it? Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: 30 June 2009 05:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] signs of good perfusion Dear Dzung, I wonder whether the perfusion would be good in an euthanized animal with the circulation arrested. It would be better to try it under deep anesthesia. Certainly perfusion would be better Regards Raghul RCC laboratories India Pvt. Ltd Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID ------------------------------ Message: 5 Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT) From: Phyllis Thaxton Subject: [Histonet] Cardboard Paraffin Catchers To: Histonet@lists.utsouthwestern.edu Message-ID: <372922.65853.qm@web43506.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone remember the little cardboard microtome trays for paraffin waste? If so please email me the company that makes them. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ------------------------------ Message: 6 Date: Mon, 29 Jun 2009 12:07:04 -0400 From: Merced M Leiker Subject: Re: [Histonet] Signs of good perfusion To: "Thach, Dzung (NIH/NIAID) [E]" , histonet@lists.utsouthwestern.edu Message-ID: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculature....ideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" wrote: > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out > blood and then perfusing through ventricle using a 21G butterfly > needle and peristaltic pump. Sometimes I see the lungs swelling up > and fluid comes out of mouth. Occasionally, I see the liver fade to > light pink. Most of the time the paws become white. But the brain > and spinal cord (my tissues of interest) are always white, and seem to > have been perfused. I was wondering how to improve this to get more > consistent good perfusions, and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 7 Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT) From: Steven Coakley Subject: [Histonet] WI/MI To: Histonet@lists.utsouthwestern.edu Message-ID: <385293.93112.qm@web38204.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I'm looking for a few techs from Northern WI or Northern MI to let me know when HT positions become availiable. Thanks ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 32 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Tue Jun 30 07:54:50 2009 From: arvidsonkristen <@t> yahoo.com (arvidsonkristen@yahoo.com) Date: Tue Jun 30 07:54:54 2009 Subject: [Histonet] Quality Manuals Message-ID: <244117.7043.qm@web65713.mail.ac4.yahoo.com> Greetings all- Thank you to everyone who sent me a copy of the CAP checklist.? I found a couple more standards I would love to get my hands on (you have to pay for them).? They are: CLSI HS 1-A2 ISO 9001 ISO 15189 AABB-Quality System Essentials ? I have learned a lot in my quest for all this info....what an undertaking!! ? Thanks again, Kristen ? From melissafphelan <@t> gmail.com Tue Jun 30 08:36:01 2009 From: melissafphelan <@t> gmail.com (Melissa Phelan) Date: Tue Jun 30 08:36:04 2009 Subject: [Histonet] Trying to Unsubscribe? Message-ID: <181bbd170906300636i16fd622bk8ad5b761a6c6a5c3@mail.gmail.com> Hello, I am trying to unsubscribe from here, but I have click on the unsubscribe button on the website and have yet to receive the email to unsubscribe. Please unsubscribe me from the list. Thank you! -- Melissa Phelan Laboratory/Pathology/Biotech Cell: 407-697-1175 UCF KNIGHTS!! From carrolpb <@t> umdnj.edu Tue Jun 30 08:48:30 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Jun 30 08:48:38 2009 Subject: [Histonet] Trying to Unsubscribe? In-Reply-To: <181bbd170906300636i16fd622bk8ad5b761a6c6a5c3@mail.gmail.com> References: <181bbd170906300636i16fd622bk8ad5b761a6c6a5c3@mail.gmail.com> Message-ID: <4A4A17AE.7070906@umdnj.edu> This list should really consider levying a fee of $1 on anyone who writes the entire list asking how to unsubscribe. The list moderator would be rich in no time :-0 Melissa Phelan wrote: > Hello, > > I am trying to unsubscribe from here, but I have click on the unsubscribe > button on the website and have yet to receive the email to unsubscribe. > Please unsubscribe me from the list. Thank you! > > From leiker <@t> buffalo.edu Tue Jun 30 09:38:53 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Jun 30 09:38:59 2009 Subject: [Histonet] RE: Signs of good perfusion In-Reply-To: <7722595275A4DD4FA225B92CDBF174A18C7DAA9AD9@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A18C7DAA9AD9@EXC-MBX3.cfs.le.ac.uk> Message-ID: <2A3E57E601F3F6B25A9746A3@CDYwxp1931.ad.med.buffalo.edu> but he's not using fixative. --On Tuesday, June 30, 2009 10:06 AM +0100 "Edwards, R.E." wrote: > A stiff tail!. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thach, > Dzung (NIH/NIAID) [E] Sent: 29 June 2009 16:31 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Signs of good perfusion > > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out of mouth. Occasionally, I see the liver fade to light pink. Most of > the time the paws become white. But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused. I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From leiker <@t> buffalo.edu Tue Jun 30 09:40:05 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Jun 30 09:40:10 2009 Subject: [Histonet] signs of good perfusion In-Reply-To: <000001c9f937$9c779550$d566bff0$@raghul@rccltd.in> References: <000001c9f937$9c779550$d566bff0$@raghul@rccltd.in> Message-ID: <3CDECA327A0B006A2A016BE0@CDYwxp1931.ad.med.buffalo.edu> i think it probably would be better; but good luck getting approval to do that. --On Tuesday, June 30, 2009 9:32 AM +0530 raghul wrote: > Dear Dzung, > > I wonder whether the perfusion would be good in an euthanized animal with > the circulation arrested. It would be better to try it under deep > anesthesia. Certainly perfusion would be better > > Regards > Raghul > RCC laboratories India Pvt. Ltd > > > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out of mouth. Occasionally, I see the liver fade to light pink. Most of > the time the paws become white. But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused. I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > > > ------------------------------ > > Message: 5 > Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT) > From: Phyllis Thaxton > Subject: [Histonet] Cardboard Paraffin Catchers > To: Histonet@lists.utsouthwestern.edu > Message-ID: <372922.65853.qm@web43506.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Does anyone remember the little cardboard microtome trays for paraffin > waste? If so please email me the company that makes them. > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > ------------------------------ > > Message: 6 > Date: Mon, 29 Jun 2009 12:07:04 -0400 > From: Merced M Leiker > Subject: Re: [Histonet] Signs of good perfusion > To: "Thach, Dzung (NIH/NIAID) [E]" , > histonet@lists.utsouthwestern.edu > Message-ID: <1D8E958050ABAD54BD852005@CDYwxp1931.ad.med.buffalo.edu> > Content-Type: text/plain; charset=us-ascii; format=flowed > > Live going pale is a good sign, your tissues of interest going pale is an > even better sign, but fluid coming out of the mouth (or even the nose, or > additionally, any kind of bloating or swelling in the animal) is a bad > sign. You may not be able to get good perfusion (pale tissues) if this > happens before your tissues turn pale, as the pressure is too high > causing fluid to leak out of the vasculature....ideally you want to push > the blood out through the the hole you made in the right atrium, not > through the walls of the vessels. at what rate do you perfuse? if this > happens a lot slow it down. > > Hope this helps. > > --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" > wrote: > >> Hi Everyone!! >> >> I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am >> nicking the upper right atrium of heart to collect the gushed out blood >> and then perfusing through ventricle using a 21G butterfly needle and >> peristaltic pump. Sometimes I see the lungs swelling up and fluid comes >> out of mouth. Occasionally, I see the liver fade to light pink. Most of >> the time the paws become white. But the brain and spinal cord (my >> tissues of interest) are always white, and seem to have been perfused. I >> was wondering how to improve this to get more consistent good perfusions, >> and what signs should I look for to indicate good perfusion? >> >> Thanks much, >> >> Dzung >> NIAID >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > > ------------------------------ > > Message: 7 > Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT) > From: Steven Coakley > Subject: [Histonet] WI/MI > To: Histonet@lists.utsouthwestern.edu > Message-ID: <385293.93112.qm@web38204.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I'm looking for a few techs from Northern WI or Northern MI to let me know > when HT positions become availiable. > > Thanks > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 67, Issue 32 > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From af46 <@t> buffalo.edu Tue Jun 30 09:40:07 2009 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Tue Jun 30 09:40:13 2009 Subject: [Histonet] long formalin fixation affecting immuno staining Message-ID: We have been working with mouse brains that were fixed in 10% formalin for one month. We are currently getting "no" staining with our immunos. Can this really be the problem in light of antigen retrieval methods? Annette Featherstone From DixonM <@t> vetmed.ufl.edu Tue Jun 30 10:36:21 2009 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Tue Jun 30 10:36:28 2009 Subject: [Histonet] Leica polycut SM2500S Message-ID: <530D827EC657DE418C3572ADD63FCDC30103279A@EXGVMCNETWORK.vetmed.ufl.edu> Hi histonetters, Well, I just acquire a Leica SM2500S polycut heavy duty microtome and could use your help. Everything seems to be working however I cannot get the knife holder to automatically descend to account for the desired micron setting in either the manual or automatic modes. The knife just moves back and forth over the block without cutting a actual section. However, in the setup mode, I am physically able to move the knife holder up and down with the arrow keys. I have the manual and have loosened and tightened the knife holder screws a million times. Unfortunately at this point I can't tell if it's something simple or that the particular mechanisms that performs this action is broken. Any help or suggestions would be greatly appreciated. I can also be contacted offline at the phone number below. Thank you all. MaryAnn MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Oncology Department UF Veterinary Medical Center Phone (352) 392-2226 Ext. 5739 From gayle.callis <@t> bresnan.net Tue Jun 30 10:38:21 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Jun 30 10:38:53 2009 Subject: [Histonet] RE: Perfusion technics for rodents Message-ID: <000101c9f998$d3795820$7a6c0860$@callis@bresnan.net> Several of our labs perfuse mice via heart (using a smaller gauge needle and syringe filled with fixative, or with larger rodents, via a peristolic(sp?) pump with PBS perfused first followed by a fixative. The animals are anesthetized via an injection first, then perfused. The perfusion itself is euthanasia and with more adult animals, it is apparent when they are euthanized/perfused when upper and lower legs extend and are stiff. With really young animals, perfusion may be more difficult. The perfusion starts through the heart since it pumps at allow the perfusion to take place. If it is difficult to see a tiny heart and exact location to insert a needle correctly, I suggest using safety glasses that are magnifiers. These are available from various vendors. With anesthetized hamsters or mouse, the abdominal area is opened to expose the ascending/descending aortas behind intestines. These blood vessels are severed to allow perfusion fluids to flow away from the animal. As far as approval to anesthetize via injection (using an approved anesthetic) and perfuse any animal, the university research laboratories have strict rules to follow. An Animal Care Committee with sets up and requires methods used that are guidelines from granting agencies (NIH, etc) These rules are adhered to faithfully or a researcher can be severely criticized, and probably with penalties in place. They have regular inspections to make sure they are doing these protocols correctly. I have watched both type of perfusions and the animal simply sleeps through the procedure. It is a carefully orchestrated, painless and humane protocol.There are some who may find this an unsavory topic, but I was impressed at how humane it was compared to the old cervical dislocation method to euthanize rodents. That is no longer allowed. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT You Wrote: it probably would be better; but good luck getting approval to do that. --On Tuesday, June 30, 2009 9:32 AM +0530 raghul <@t> rccltd.in> wrote: > Dear Dzung, > > I wonder whether the perfusion would be good in an euthanized animal with > the circulation arrested. It would be better to try it under deep > anesthesia. Certainly perfusion would be better > > Regards > Raghul > RCC laboratories India Pvt. Ltd > > > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out of mouth. Occasionally, I see the liver fade to light pink. Most of > the time the paws become white. But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused. I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > From gayle.callis <@t> bresnan.net Tue Jun 30 10:51:20 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Jun 30 10:51:47 2009 Subject: [Histonet] RE: mouse brain fixation Attn: Annette Featherstone Message-ID: <000601c9f99a$9db64de0$d922e9a0$@callis@bresnan.net> Annette, You did not say what immunos e.g. antigens you are trying to stain? Nor how you are doing the actual staining method? More information would help please. If you are trying to stain for murine CD markers or some other cellular antigens, there are not many that work after FFPE. If you need to know this information, go to SEROTEC and look at murine CD or other cellular markers, and see if the antibody will work with FFPE paraffin applications. BD Bioscience also has applications that work, tested, reported, IHC or frozen sections and found in their Technical Data Sheets for any given antibody. There are some other fixatives to try for CD and other cellular markers - SEROTEC often refers to PLP (paraformaldehyde-lysine-periodate with recipe found on web through IHCWorld or Immunoportal, Google the keywords) while BD Bioscience refers to Becksteads Formalin free Zn TRIS buffer fixative. If you need the recipe for that, I will be happy to send. Otherwise BD Bioscience would be happy to sell that to you but it is cheaper and easy to make up in the lab. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT You wrote: We have been working with mouse brains that were fixed in 10% formalin for one month. We are currently getting "no" staining with our immunos. Can this really be the problem in light of antigen retrieval methods? Annette Featherstone From ploykasek <@t> phenopath.com Tue Jun 30 12:23:36 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jun 30 12:23:45 2009 Subject: [Histonet] Used equip. Message-ID: Thanks to all who replied to my inquiry on used equipment dealers. I have received good information. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From rosenfeldtek <@t> hotmail.com Tue Jun 30 12:48:46 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Jun 30 12:48:59 2009 Subject: [Histonet] Good Perfusion... Message-ID: No, I wouldn't nick the atrium. Instead, cut the femoral artery at the groin. And of course, you cannot perfuse a living, anesthetized animal. That would be unethical and also illegal. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jun 30 12:52:32 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jun 30 12:52:40 2009 Subject: [Histonet] Leica Autostainer XL Problems In-Reply-To: <6D3E01C097DB5C479B25F4063F6385CB03728678@PMC-EMAIL.petermac.org.au> Message-ID: Steve You need to keep you reagent buckets filled to the middle line. The 100% alcohol should be to the maximum line especially after the eosin. You have some running of solution down the top of the slide causing uneven staining. When we hand stain we move the baskets vigorously up and down. That will take care of any run off of solution. Automation is a wonderful thing but it can only work as well as it is set up to operate. Hence the suggestion to fill some buckets with less solution and the 100% with maximum amount. 100% will clear the top of the slides so solution will not run down the slide or so you won't have uneven staining at the top of the slide. Hope this makes sense. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Asquith Stephen" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Leica Autostainer XL Problems 06/29/2009 08:24 PM Hi All, We have a Leica Autostainer XL that we do all our routine HE slides on. The section on the upper end of the slide is often unevenly stained. We have tried many things to improve the situation including; Ensuring wash tubs are flowing correctly and Increasing dips at each staining tub. If we "use" the same program but do it by hand the problem goes away. Any suggestion that may help would be most welcome. Steve Stephen Asquith Microscopy Imaging and Research Core Facility Peter MacCallum Cancer Centre Locked Bag #1 A'Beckett Street East Melbourne 8006 Email: stephen.asquith@petermac.org Phone +61-3-9656 1244/1243 Fax +61-3-9656 1411 This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jfish <@t> gladstone.ucsf.edu Tue Jun 30 13:37:07 2009 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Tue Jun 30 13:37:16 2009 Subject: [Histonet] Good Perfusion... In-Reply-To: References: Message-ID: <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> Dear Jerry and histonetters, I don't believe this is unethical or illegal. It is written into our IACUC approved protocols as follows: Chemical Method of Euthanasia: "Perfusion under general anesthesia, Avertin or Halothane induced. Bilateral thoracotomy." Could it only be forbidden by your facility? I wonder if other facilities have rules against such things. But we insist that our investigators use perfusion under anesthsia because the deeper tissues are much better fixed and blood removal is more thorough and complete by this method. Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Tuesday, June 30, 2009 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Perfusion... No, I wouldn't nick the atrium. Instead, cut the femoral artery at the groin. And of course, you cannot perfuse a living, anesthetized animal. That would be unethical and also illegal. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Insert movie times and more without leaving HotmailR. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Tue Jun 30 14:17:51 2009 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Tue Jun 30 14:17:57 2009 Subject: [Histonet] Iron for Blood Smears & Urine Message-ID: Hi histo world.. I hate to be stupid, but if you'd have dealt with the cable TV company today like I did....your mind would be blank too....But now I'm back to work, after wasting 5 hours and I STILL don't have internet, phone or TV. I don't think I can handle one more day of this...I'm going through withdrawal! So, here goes: I have 2 bone marrow smears. I have an iron control that I've run down to water. I have to put BOTH of them in Methanol (the dry smear AND the wet control) . . before I air dry and then put in heated Ferrocyanide? Right? I have the procedure here, but it doesn't state BOTH slides- the procedure makes me believe that I don't put both slides in methanol . . . and I haven't done this stain in about 3 years, so I'm a little rusty :-) Thank you for helping out a forgetful (and irritated!) tech! Amy Senn, HT Histology Laboratory 717-763-2124 Don't ever question the value of volunteers. Noah's Ark was built by volunteers; the Titanic was built by professionals Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From chana.de.wolf <@t> gmail.com Tue Jun 30 14:37:20 2009 From: chana.de.wolf <@t> gmail.com (Chana de Wolf) Date: Tue Jun 30 14:37:24 2009 Subject: [Histonet] Good Perfusion... In-Reply-To: <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> References: <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> Message-ID: <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish wrote: > Dear Jerry and histonetters, > > I don't believe this is unethical or illegal. It is written into our IACUC > approved protocols as follows: > > Chemical Method of Euthanasia: "Perfusion under general anesthesia, > Avertin > or Halothane induced. Bilateral thoracotomy." > > Could it only be forbidden by your facility? > > I wonder if other facilities have rules against such things. But we insist > that our investigators use perfusion under anesthsia because the deeper > tissues are much better fixed and blood removal is more thorough and > complete by this method. > > Jo Dee > > > ~~Jo Dee Fish~~ > Senior Research Technologist > The J. David Gladstone Institutes > Co-manager Histology and Microscopy Core > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R > Sent: Tuesday, June 30, 2009 10:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Good Perfusion... > > > No, I wouldn't nick the atrium. > > Instead, cut the femoral artery at the groin. > > And of course, you cannot perfuse a living, anesthetized animal. That > would > be unethical and also illegal. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Insert movie times and more without leaving HotmailR. > > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori > al_QuickAdd_062009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rosenfeldtek <@t> hotmail.com Tue Jun 30 15:38:48 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Jun 30 15:38:53 2009 Subject: [Histonet] Good Perfusion--I stand corrected. In-Reply-To: <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> References: <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> Message-ID: I stand correct on the perfusion under anesthesia issue. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Tue, 30 Jun 2009 12:37:20 -0700 Subject: Re: [Histonet] Good Perfusion... From: chana.de.wolf@gmail.com To: jfish@gladstone.ucsf.edu CC: rosenfeldtek@hotmail.com; histonet@lists.utsouthwestern.edu Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish wrote: Dear Jerry and histonetters, I don't believe this is unethical or illegal. It is written into our IACUC approved protocols as follows: Chemical Method of Euthanasia: "Perfusion under general anesthesia, Avertin or Halothane induced. Bilateral thoracotomy." Could it only be forbidden by your facility? I wonder if other facilities have rules against such things. But we insist that our investigators use perfusion under anesthsia because the deeper tissues are much better fixed and blood removal is more thorough and complete by this method. Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Tuesday, June 30, 2009 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Perfusion... No, I wouldn't nick the atrium. Instead, cut the femoral artery at the groin. And of course, you cannot perfuse a living, anesthetized animal. That would be unethical and also illegal. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Insert movie times and more without leaving HotmailR. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From CIngles <@t> uwhealth.org Tue Jun 30 16:29:29 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Jun 30 16:29:33 2009 Subject: [Histonet] Good Perfusion... References: <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> Message-ID: Sorry, but it is against my personal ethics (and/or morals?) to do such a thing. Guess I'll never get to work in research... But then I can't even pith a frog without squirming. After it's dead though, nothing bothers me. (OK, maybe eyes) Perhaps I'm just wierd. (oh, wait, I work in a lab...) Like I always try to explain to the pathology residents who are trying to cram 3cmx3cmx5mm pieces of breast tissue into a cassette. "Just because you can, doesn't mean you should." Don't get me wrong, I DON'T volunteer with PETA, etc. and know animal models are instrumental to many research programs. But I have a hard time figuring out where gray area turns to black. Let the flaming begin. And it's only Tuesday. Boy I am on a roll! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chana de Wolf Sent: Tue 6/30/2009 2:37 PM To: jfish@gladstone.ucsf.edu Cc: histonet@lists.utsouthwestern.edu; JR R Subject: Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf From chana.de.wolf <@t> gmail.com Tue Jun 30 20:25:06 2009 From: chana.de.wolf <@t> gmail.com (Chana de Wolf) Date: Tue Jun 30 20:25:11 2009 Subject: [Histonet] Good Perfusion... In-Reply-To: References: <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> Message-ID: <3f4b71f10906301825l26712686m90249c1e50a3ffd3@mail.gmail.com> Actually, as I explained, in the case of perfusion fixation, you can *and* you should begin perfusion pre-mortem if you wish to measure anything other than ischemic injury. That's pretty much the point. Your own personal feelings aside, it is extremely important that people on this list who are not familiar with standard perfusion protocol do not get the erroneous idea that such procedures are illegal or inhumane. The animal is deeply anesthetized prior to the procedure and death is painless. While not the most aesthetically attractive method of euthanasia, it is simply a thoracotomy followed by exsanguination, which is an AVMA approved method of euthanasia when used in combination with sedation or anesthesia. I am not interested in "flaming," simply in clarifying. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 2:29 PM, Ingles Claire wrote: > Sorry, but it is against my personal ethics (and/or morals?) to do such a > thing. Guess I'll never get to work in research... But then I can't even > pith a frog without squirming. After it's dead though, nothing bothers me. > (OK, maybe eyes) Perhaps I'm just wierd. (oh, wait, I work in a lab...) > Like I always try to explain to the pathology residents who are trying to > cram 3cmx3cmx5mm pieces of breast tissue into a cassette. "Just because you > can, doesn't mean you should." Don't get me wrong, I DON'T volunteer with > PETA, etc. and know animal models are instrumental to many research > programs. But I have a hard time figuring out where gray area turns to > black. > Let the flaming begin. And it's only Tuesday. Boy I am on a roll! > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chana de Wolf > Sent: Tue 6/30/2009 2:37 PM > To: jfish@gladstone.ucsf.edu > Cc: histonet@lists.utsouthwestern.edu; JR R > Subject: Re: [Histonet] Good Perfusion... > > > > Histonetters, > > Perfusion under deep anesthesia is most certainly not unethical NOR > illegal, > and, in fact (as mentioned by Jo Dee), it is necessary for optimal > perfusion > and fixation -- the intracellular ischemic cascade begins immediately upon > circulatory arrest, setting off a chain of events highly detrimental to > subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" > phenomenon, effectively guaranteeing that you are not perfusing all tissues > adequately at all! It is therefore *most* beneficial to begin perfusion > with > a beating heart, and every perfusion protocol I have ever worked under > requires it (especially if the tissues are to be used for EM). > > I perform around 5-10 perfusions per week *specifically* to study the > effects of ischemia on reperfusion and neural ultrastructure. > > Of course, you should check with your institution's particular rules and > regulations, but perfusion begun under anesthesia is scientifically > justified for the reasons mentioned above and should therefore be > relatively > easy to have approved by your IACUC. > > Sincerely, > > Chana de Wolf > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >