[Histonet] Please Help!

[Stacey Barrick]

You might have some luck trying this - 
Wipe out the cryostat with a dryer sheet to remove the static. This trick works for me alot. Sometimes you need to repeat it a few times during cutting. Wiping the cold plate withthe dryer sheet works wonders for me! 
 
Good Luck!!
Stacey

--- On Fri, 7/31/09, DELIA GARCIA <deliadfam <@t> yahoo.com> wrote:


From: DELIA GARCIA <deliadfam <@t> yahoo.com>
Subject: [Histonet] Please Help!
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, July 31, 2009, 2:41 PM


Hello,

I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much.

Delia



      
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