[Histonet] Re: Tissue Washing

[Hobbs, Carl]

Again, apologies if I have messed up with message delivery : Firefox from IE is a learning curve after IE for many years.

Re your problem: only part that I reckon that is your problem is ONLY using ~20mins in an oven.
Leave for 2hrs at 60C, then stain.( overnight if you can is great for H&Es)

See if this makes any difference.
Then take them out of the oven and straight into Xylene....no need to cool: if they are going to stay on, cooling will play NO part in additional adhesion.
Occam.