[Histonet] Mouse Eyes

[Rene J Buesa]

I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration.
René J.

--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca> wrote:


From: Jo-Ann Bader, Ms. <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Mouse Eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 29, 2009, 11:38 AM


We have a new project pending with mouse eyes.  The lens must remain intact.  What is the  best method to process, embed and cut them?  Paraffin or glycol methacrylate?



Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University
1600 Pine Ave W. 
Room 312
Montreal, QC, Canada
H3G 1Y6
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