[Histonet] immunofluorescence labeling on JB-4 embedded tissues

[Jurcisek, Joseph]

I need to run some immunofluorescent labeling on some tissue which was
embedded in JB-4. Does anyone have a protocol?  My boss thinks I need to
etch the plastic beforehand.  Thanks.

Joe

Joseph A. Jurcisek
Senior Research Associate
Center for Microbial Pathogenesis
The Research Institute at Nationwide Children's Hospital
700 Children's Dr.
Columbus, OH 43205-2696
(614) 355-3521
fax (614) 722-2818
Joseph.Jurcisek <@t> nationwidechildrens.org



----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.