[Histonet] Sakura Rapid Tissue Processor

[Bryan Hewlett]

Daniel,

You wrote;
>>That said, is there a reason why we couldn't or shouldn't
> process breast core Bx's on the Xpress provided they've been sitting
> overnight in formalin?

See following article.
Concensus Recomendations on ER testing in breast cancer by IHC(Appl 
Immunohistochem Mol Morphol 2008;16:513-520)


Bryan

---- Original Message ----- 
From: "Daniel Schneider" <dlschneider <@t> gmail.com>
To: "Jean Warren" <jwarren23 <@t> cinci.rr.com>
Cc: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 28, 2009 10:01 AM
Subject: Re: [Histonet] Sakura Rapid Tissue Processor


> Actually, that was perfect, wordy replies are what I had hoped for.
>
> How is the automated embedding working out for you?  The way I see things,
> automated embedding is the true killer feature of the Xpress -- that is, 
> if
> it works.
>
> Why don't skins process well on the Xpress?  (When I first appreciated the
> automated embedding feature, my first thought was "Great, no more
> misembedded pigmented skin lesions!"  But if we can't do skins, well...)
>
> Breasts cancers require at least 8 hours formalin fixation for reliable
> Her2Neu FISH; presumably that's why you've been told not to do breasts on
> the Xpress.  That said, is there a reason why we couldn't or shouldn't
> process breast core Bx's on the Xpress provided they've been sitting
> overnight in formalin?
> Thanks!!!
> Daniel Schneider
> On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren <jwarren23 <@t> cinci.rr.com> 
> wrote:
>
>> We have the Sakura rapid tissue processor at my hospital lab, which is a
>> large private hospital. We have had it three years and it has been 
>> somewhat
>> of a disappointment::
>>
>> We have been told that you should not process breasts in it, because you
>> will not get reliable
>> results for FISH.
>>
>> Its implementation has created schedule changes that have caused some 
>> good
>> techs to leave.
>>
>> The scenario to get a case out the same day is rare.
>> If a patient has surgery at 7 am and we receive it by 8 am, it is
>> accessioned
>> and grossed in.
>> Except for biopsies, the specimen still will need 2 hours fixation in
>> formalin. When
>> we receive it in Histology at 1030 am, it must go in pre-processing
>> solution
>> for 30 minutes. At 1100, we process it for approx 1 hour.
>> Then embed, cut and stain. Our docs would get it well after lunch and, if
>> all is
>> OK, they can get the report out.
>> And, there are not many cases that meet that time criterion.
>>
>> One other drawback is that it is more labor intensive to handle 10 blocks
>> ten times a day than to handle 100 at one time. Our lab processes from
>> 400-750 blocks a day and less than 100 a day are processed on that
>> processor. All we are handling on the instrument are bxs, bone marrows 
>> and
>> cytology blocks. If too large a specimen is placed on it,  we usually 
>> have a
>> problem. Endometrial bxs, skins and cones have not had favorable results.
>>
>> On the other hand, biopsies, especially livers, look and cut better. Our
>> hematology expert wants all bone marrows done that way. We also rapid
>> process most cytology that way, but bloody cases still need formalin
>> fixation.
>>
>> It is much simpler to change the processor ( and more expensive ).
>>
>> We tried to do most specimens on it with terrible failure. If anyone gets
>> it, I
>> would recommend starting slowly with a few specimen types and gradually
>> adding.
>>
>> Our hope is that it will be more useful in the future.
>>
>> I know, rather wordy answer!
>>
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