[Histonet] GMS-Fungus on nails

[Ingles Claire]

We always run a PAS(manual) on our nails. Alot less harsh than GMS. We cut doubles and automatically stain them in case one falls off, and we always use plus slides.
Claire

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From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Clare Thornton
Sent: Fri 7/24/2009 10:30 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] GMS-Fungus on nails



We do a GMS for fungus on all nails we receive.  We often have a difficult time keeping the nail tissue on the slide.  We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck.  Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at.  We use the Artisan stainer for our specials.  We are really not interested in performing the GMS manually, due to volume and turn around time restrictions.  Any suggestions?

Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com

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