[Histonet] von kossa
Madary, Joseph
MadaryJ <@t> MedImmune.com
Fri Jul 17 12:15:41 CDT 2009
I did a von kossa day the other since it was sunny out and never thought to use a hema counterstain. I will try that next time! I use ammonia water too as a bluing agent.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, July 17, 2009 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 21
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Today's Topics:
1. Hi Jan, (Carrie Disbrow)
2. Quality Stuff (kristen arvidson)
3. RE: Quality Stuff (Mike Pence)
4. Re: Quality Stuff (Rene J Buesa)
5. Re: Biological hood with grossing station (Joseph Saby)
6. RE: Hi Jan, (Tony Henwood)
7. Re: Hi Jan, (Victor Tobias)
8. Re: Quality Stuff (thecitan <@t> yahoo.com)
9. VonKossa's calcium stain (karine cadoret)
10. RE: VonKossa's calcium stain (Tony Henwood)
11. Re: VonKossa's calcium stain (Jack Ratliff)
12. Protocol for fluorescence of myofibers
(J.P.H.M. van den Wijngaard)
13. NSH Meeting in Alabama (mtitford <@t> aol.com)
----------------------------------------------------------------------
Message: 1
Date: Thu, 16 Jul 2009 16:15:24 -0400
From: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
Subject: [Histonet] Hi Jan,
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A5F521B.72AC.0059.0 <@t> shands.ufl.edu>
Content-Type: text/plain; charset=US-ASCII
Hi Jan,
Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management!
The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one!
Carrie
------------------------------
Message: 2
Date: Thu, 16 Jul 2009 14:14:36 -0700 (PDT)
From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
Subject: [Histonet] Quality Stuff
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <685857.31127.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input.
------------------------------
Message: 3
Date: Thu, 16 Jul 2009 16:21:00 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Quality Stuff
To: "kristen arvidson" <arvidsonkristen <@t> yahoo.com>, "histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A3BBD <@t> is-e2k3.grhs.net>
Content-Type: text/plain; charset="iso-8859-1"
There is NO margin of error acceptable in mislabeling blocks or slides. I expect 100% compliance with this in my department. When you have like specimens all day like derm, you cannot make labeling errors.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Thursday, July 16, 2009 4:15 PM
To: histonet
Subject: [Histonet] Quality Stuff
Hello,
I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 16 Jul 2009 14:47:23 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Quality Stuff
To: histonet <histonet <@t> lists.utsouthwestern.edu>, kristen arvidson
<arvidsonkristen <@t> yahoo.com>
Message-ID: <720913.73313.qm <@t> web65707.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=utf-8
There are no acceptable "standards" for mistakes. The present tendency of implementing the "6σ method" in the lab is to precisely eliminate mistakes, not to set an "acceptable" limit.
René J.
--- On Thu, 7/16/09, kristen arvidson <arvidsonkristen <@t> yahoo.com> wrote:
From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
Subject: [Histonet] Quality Stuff
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, July 16, 2009, 5:14 PM
Hello,
I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 16 Jul 2009 14:48:30 -0700 (PDT)
From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
Subject: Re: [Histonet] Biological hood with grossing station
To: Golden State Acrylic Designs <gsacrylicdesigns <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <143120.31950.qm <@t> web33807.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Although I really like MOPEK, another source for the East Coast would be TBJ.
________________________________
From: Golden State Acrylic Designs <gsacrylicdesigns <@t> gmail.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, July 16, 2009 9:34:56 AM
Subject: [Histonet] Biological hood with grossing station
Is the a source for a biological hood with grossing station othe than
(Thermo-Fisher)
Thanks
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Fri, 17 Jul 2009 08:42:48 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Hi Jan,
To: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB03F7 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Hi Carrie,
The NSH convention in Alabama will also be my first.
It has only taken me 30 years to finally get to one.
Assuming I don't get lost I hope to see you all there (?turn right at
Honolulu, left at Los Angeles, then second exit on the left?)
I'll be the short tubby man with the funny accent.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carrie
Disbrow
Sent: Friday, 17 July 2009 6:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Hi Jan,
Hi Jan,
Thanks for your input! I have two A. S. degrees. One in veterinary
nursing/technology and one in histology. And I have an AA where all my
electives were biology,chemistry and micro. Then I'll have a BS in
veterinary nursing/management. I'm starting a molecular program in
January. So, I have a strong skills in courses you mentioned. I'm sure I
want to do the R & D techniques but not so sure about the management!
The other thing about a histology career is learning how vast the field
is. Did anyone ever have a counselor in their program that explained the
different types of positions? I'm looking forward to attending the NSH
convention in October. It will be my first one! Carrie
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
This note also confirms that this email message has been
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------------------------------
Message: 7
Date: Thu, 16 Jul 2009 15:49:39 -0700
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] Hi Jan,
To: Tony Henwood <AnthonyH <@t> chw.edu.au>
Cc: Carrie Disbrow <disbrc <@t> shands.ufl.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <4A5FAE83.30703 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Tony,
LOL, someone with a funny accent in Alabama. Something only a local can
appreciate.
Victor
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Tony Henwood wrote:
> Hi Carrie,
>
> The NSH convention in Alabama will also be my first.
> It has only taken me 30 years to finally get to one.
>
> Assuming I don't get lost I hope to see you all there (?turn right at
> Honolulu, left at Los Angeles, then second exit on the left?)
>
> I'll be the short tubby man with the funny accent.
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carrie
> Disbrow
> Sent: Friday, 17 July 2009 6:15 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hi Jan,
>
>
> Hi Jan,
> Thanks for your input! I have two A. S. degrees. One in veterinary
> nursing/technology and one in histology. And I have an AA where all my
> electives were biology,chemistry and micro. Then I'll have a BS in
> veterinary nursing/management. I'm starting a molecular program in
> January. So, I have a strong skills in courses you mentioned. I'm sure I
> want to do the R & D techniques but not so sure about the management!
> The other thing about a histology career is learning how vast the field
> is. Did anyone ever have a counselor in their program that explained the
> different types of positions? I'm looking forward to attending the NSH
> convention in October. It will be my first one! Carrie
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************
> This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been
> virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
> **********************************************************************
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 8
Date: Fri, 17 Jul 2009 00:37:34 +0000
From: thecitan <@t> yahoo.com
Subject: Re: [Histonet] Quality Stuff
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1122935475-1247791003-cardhu_decombobulator_blackberry.rim.net-1531702201- <@t> bxe1123.bisx.prod.on.blackberry>
Content-Type: text/plain; charset="utf-8"
I also run a derm lab where we gross and write cassettes. The doctors medical assistants make mistakes every week so I set up a double checking system where one tech accessions and check numbers and writes slides. Then I gross and make one final qc check. this is only possible since I have low volume - not too sure about specific setups for larger lab qc
Sent from my Verizon Wireless BlackBerry
-----Original Message-----
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Date: Thu, 16 Jul 2009 14:47:23
To: histonet<histonet <@t> lists.utsouthwestern.edu>; kristen arvidson<arvidsonkristen <@t> yahoo.com>
Subject: Re: [Histonet] Quality Stuff
There are no acceptable "standards" for mistakes. The present tendency of implementing the "6σ method" in the lab is to precisely eliminate mistakes, not to set an "acceptable" limit.
René J.
--- On Thu, 7/16/09, kristen arvidson <arvidsonkristen <@t> yahoo.com> wrote:
From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
Subject: [Histonet] Quality Stuff
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, July 16, 2009, 5:14 PM
Hello,
I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 9
Date: Fri, 17 Jul 2009 12:34:51 +1000 (EST)
From: "karine cadoret" <kcadoret <@t> amc.edu.au>
Subject: [Histonet] VonKossa's calcium stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001001ca0687$296b2600$7c417200$@edu.au>
Content-Type: text/plain; charset="US-ASCII"
Hi,
When doing a VonKossa stain in order to demonstrate calcium in tissue,
does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
hematoxylin (which takes 6 months to ripen) ?
Also, can I simply use homemade scott's tapwater for blueing instead of
using a lithium carbonate solution ?
Thank you for your help,
Karine Cadoret
Fish health laboratory manager
National Center for Marine Conservation and Resource Sustainability
Newnham, TAS
Australia
------------------------------
Message: 10
Date: Fri, 17 Jul 2009 13:43:37 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] VonKossa's calcium stain
To: "karine cadoret" <kcadoret <@t> amc.edu.au>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB03FF <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Karine,
Either Hx will do, though I would not have used a haematoxylin since it
will lake with the calcium forming a blue stained deposit. I would
expect it to mask the silver of the von-kossa stain.
I would recommend 1% neutral red, ethylene green or even a light eosin
counterstain.
The silver "stained" calcium deposits should then stand out quite well.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of karine
cadoret
Sent: Friday, 17 July 2009 12:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] VonKossa's calcium stain
Hi,
When doing a VonKossa stain in order to demonstrate calcium in tissue,
does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
hematoxylin (which takes 6 months to ripen) ?
Also, can I simply use homemade scott's tapwater for blueing instead of
using a lithium carbonate solution ?
Thank you for your help,
Karine Cadoret
Fish health laboratory manager
National Center for Marine Conservation and Resource Sustainability
Newnham, TAS
Australia
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************
------------------------------
Message: 11
Date: Thu, 16 Jul 2009 23:15:37 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: Re: [Histonet] VonKossa's calcium stain
To: karine cadoret <kcadoret <@t> amc.edu.au>
Cc: "<histonet <@t> lists.utsouthwestern.edu>"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU0-SMTP1843C194DF1F376FD822D5AE1E0 <@t> phx.gbl>
Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
What is your tissue of interest? Why not do the Von Kossa stain first
and then counterstain with MacNeal's tetrachrome. This way you employ
the use of a metachromatic stain for the rest of the tissue instead of
just a nuclear staining hematoxylin.
Jack
On Jul 16, 2009, at 9:34 PM, "karine cadoret" <kcadoret <@t> amc.edu.au>
wrote:
> Hi,
>
> When doing a VonKossa stain in order to demonstrate calcium in tissue,
> does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
> hematoxylin (which takes 6 months to ripen) ?
>
> Also, can I simply use homemade scott's tapwater for blueing instead
> of
> using a lithium carbonate solution ?
>
>
>
> Thank you for your help,
>
>
>
> Karine Cadoret
>
> Fish health laboratory manager
>
> National Center for Marine Conservation and Resource Sustainability
>
> Newnham, TAS
>
> Australia
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 12
Date: Fri, 17 Jul 2009 15:38:38 +0100
From: "J.P.H.M. van den Wijngaard" <J.P.vandenWijngaard <@t> amc.uva.nl>
Subject: [Histonet] Protocol for fluorescence of myofibers
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <ba9cb7db4f0c4577.4a609afe <@t> amc.uva.nl>
Content-Type: text/plain; charset=us-ascii
Dear experts of histonet,
Given my formal background (chemistry synthesis) and current experience (cardiovascular research) my question may seem either too simple of not well directed. Please forgive my inexperience in these matters.
In short, I am looking for a simple method that enhances the fluorescence of the cardio myofibers.
In our institution, we have constructed a special setup allowing for investigation of vessel morphology. This is carried out by infusing a fluorescent plastic that polymerizes into an organ and then serially slicing the specimen while after each slice a high resolution image is taken of the remaining bulk material. As such, we can create high resolution 3D images of the vasculature, e.g. of a heart or kidney.
Recently we have extended our setup (we are using a 16mpixel cooled camera which also allows very long exposure times) and are now trying to visualize the muscle fibers of the heart. For this we use a powerled (around 400nm) and image at around 600nm which seems to generates images that show autofluorescence of either collagen or muscle (I am unsure which this may be). Given these initial promising results, I would like to visualize the muscle fibers in more detail by using a staining protocol that would allow to stain post mortem hearts. I have gathered information so far that includes the use of ALA or Bouin's solution but there may be much better protocols suitable for this problem.
I appreciate all feedback, thank you in advance,
Jeroen
Jeroen PHM van den Wijngaard, PhD
Department of Biomedical Engineering and Physics
Academic Medical Center
Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
Tel: +31 (20) 5668796
------------------------------
Message: 13
Date: Fri, 17 Jul 2009 11:33:34 -0400
From: mtitford <@t> aol.com
Subject: [Histonet] NSH Meeting in Alabama
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CBD50B14F6AE2E-EF8-3E18 <@t> webmail-md01.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings!
Michael Titford
Pathology USA
Mobile AL
------------------------------
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End of Histonet Digest, Vol 68, Issue 21
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