[Histonet] VonKossa's calcium stain

[Tony Henwood]

Karine,

Either Hx will do, though I would not have used a haematoxylin since it
will lake with the calcium forming a blue stained deposit. I would
expect it to mask the silver of the von-kossa stain.

I would recommend 1% neutral red, ethylene green or even a light eosin
counterstain.
The silver "stained" calcium deposits should then stand out quite well.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of karine
cadoret
Sent: Friday, 17 July 2009 12:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] VonKossa's calcium stain


Hi, 

When doing a VonKossa stain in order to demonstrate calcium in tissue,
does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
hematoxylin (which takes 6 months to ripen) ? 

Also, can I simply use homemade scott's tapwater for blueing instead of
using a lithium carbonate solution ? 

 

Thank you for your help, 

 

Karine Cadoret

Fish health laboratory manager

National Center for Marine Conservation and Resource Sustainability

Newnham, TAS

Australia 

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