[Histonet] Antigen retrieval

[Mike Tighe]

I am attempting to do IF on FFPE sections but the history/treatment of my tissues (mouse tissues) is unknown. I would like to recommend to our labs a strategy for tissue collection, length of time in NBF, type of antigen retrieval, and so on. Would anyone (or everyone) be willing to share their opinions on the best methods they have for maintaining the ability to do IF or IHC.

Thanks for any help!
Mike