[Histonet] (EDTA)Decal Bone marrows

[Akemi Allison-Tacha]

Hi Patti,
Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a Hematopathologist at NIH.  This formula was particularly great for BM Core BX's.  It is much gentler on the tissue, and is less likely to harm the cellular integrity.  Dr. Todd Barry also prefers the use of EDTA formula's for BM Bx's.

Below is the formula. or you can purchase from Biocare Medical. You should decal the specimen for 30 minutes to 1 hour and check for decalcification.  DO NOT leave for any longer than 1 hour.

10% EDTA (Ethyl-media minetetra-acetic-acid
Stock EDTA 10 ml
DI water       90 ml
pH 4.5-5.0 with Sodium Hydroxide

If you prefer to purchase the IED Solution, Biocare Medical has it available.  They have 2 sizes 140 ml or 500 ml.  cat # IED 1204.
Ion-Exchange Decalcification Unit  (IED Unit, Designed for Bone Marrow  Biopsies) 

An advanced decalcification system that removes calcium from bone
quickly while leaving superior cellular detail. The IED Unit
incorporates a strong cation ion-exchange resin in a weak acid solution
to remove calcium ions from bone, replacing them with hydrogen ions.
Because the IED Unit does not require strong concentrated acid
solutions, as in traditional decalcification methods, delicate cellular
structures antigenicity remain intact.

Regards,
Akemi

Akemi Allison-Tacha BS, 
HT(ASCP)HTL
Histology 
Manager
APMG 
Laboratories
105A Cooper Court, Los Gatos, CA 
95032
Contact: 
800.848.2764
V/M: 
408.884.2718
Fax: 
408.884.2758
Cell: 
408.335.9994
E-Mail: aallison-tacha <@t> apmglab.com





--- On Fri, 7/10/09, Patti Loykasek <ploykasek <@t> phenopath.com> wrote:

From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Bone marrows
To: "histonet" <histonet <@t> pathology.swmed.edu>
Date: Friday, July 10, 2009, 12:38 PM

Hi All. I was wondering what everyone's current favorite bone marrow decal
solution/method is. Including commercially available decal solutions. We
will be doing IHC after fixation/decal. Thanks for the input (as always).


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA




This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet