[Histonet] DAKO Liquid DAB

[Patsy Ruegg]

I use Stable Dab from Open Biosystems, no mixing, you keep it in a freezer
-20 but it does not freeze, it has methanol or glycerine or something in it
that keeps it from freezing, you should not freeze DAB solutions.  I love it
and it is a lot cheaper than using Dako or anyone else.  I also buy antibody
diluent, protein block and digestion reagents from Open Biosystems.  Their
website and catalogs do not list these reagents so you have to call and ask
for them.  They make a lot of products for molecular work, probes etc.  I
think this IHC stuff is sort of a side line for them.  These reagents were
first developed by David Bragatti who is now passed.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Thursday, July 09, 2009 4:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DAKO Liquid DAB

DAKO is discontinuing its product 'Liquid DAB' and wants its clients to
use the Liquid DAB+ in its place.  I have tried this product before and
feel it gives too strong a signal.  To use it I will have to re-do all
my protocols and hence revalidate all my IHC.  Can anyone suggest
another supplier that has a liquid DAB that is similar in strength to
the DAKO product?

 

Thanks for you input.

 

Joanne Clark, HT

Histology Supervisor

Pathology Consultants of New Mexico

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