[Histonet] methylene blue microinjection

[John Kiernan]

Dear Salim Yalcin Inan,
 
You need to explain your question! Electrodes are not ordinarily positioned to stimulate or record from the CSF in the ventricles of the brain. Methylene blue is soluble in water (see the Merck Index, Conn's Biological Stains or any other book about dyes or stains published since about 1885). How could injecting a soluble dye into one of the ventricles of the brain lead to "validation of drug/electrode injection/placement accuracy"? 
 
Methylene blue is listed in the catalogues of almost all vendors of chemicals because this dye has many uses. A Google Scholar search for "methylene blue" brings up "about 294,000 hits". You won't get the answers you need without doing a proper survey of earlier published literature (peer-reviewed papers, scholarly books etc) targeted at the questions you are trying to answer. 
 
Your email address shows that you are at a major Canadian university, but you don't declare yourself as a professor, postdoc, technician, graduate student, summer student, janitor's brush carrier or senior administrator. When asking for help you need to say who you are, as well as explain clearly what information you need. A well prepared question usually draws several answers that are helpful also to other people reading the listserver.
 
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada
= = =
----- Original Message -----
From: Salim Yalcin Inan <syinan <@t> ucalgary.ca>
Date: Tuesday, July 7, 2009 16:52
Subject: [Histonet] methylene blue microinjection
To: Histonet <histonet <@t> lists.utsouthwestern.edu>

> Dear All,
> 
>  
> 
> Does anyone use intracerebroventricular methylene blue injection 
> for the
> validation of drug/electrode injection/placement accuracy?
> 
> Could you please give me the catalogue number of methylene blue, 
> so I can
> order for myself? And any other advices would be greatly appreciated.
> 
> Thank you very much in advance.
> 
> Salim
> 
> syinan <@t> ucalgary.ca
> 
>  
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet