[Histonet] Help With Hemo Fading

[louise renton]

Even long term exposure to indoor lighting (fluorescent type) will fade
sectiions. We work in a building where ther lights are permanently on. If we
want to keep our sections fresh and helathy, they get covered up as soon as
.

best regards

On Fri, Jul 3, 2009 at 9:09 AM, Kemlo Rogerson <
Kemlo.Rogerson <@t> waht.swest.nhs.uk> wrote:

> I agree...... Also you're not storing them in sunlight are you? Silly
> question I know.
>
>
>
> Kemlo Rogerson
> e-mail kemlorogerson <@t> nhs.net if not at work.
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: 02 July 2009 20:28
> To: histonet <@t> lists.utsouthwestern.edu; srnty <@t> aol.com
> Subject: Re: [Histonet] Help With Hemo Fading
>
> The fading most probably is caused by acid in the permanent slide, probably
> because the sections were passed through the alcohols very quickly after the
> acid differentiation, or they stayed little time in tap water after
> differentiation or no bluing agent was used.
> It is unlikely that the mounting medium is acidic, although that could also
> be the cause also. An acid environment over the cover slipped section is the
> most probable "culprit" for the henatoxylin fading.
> Check the staining protocol.
> René J.
>
> --- On Thu, 7/2/09, srnty <@t> aol.com <srnty <@t> aol.com> wrote:
>
>
> From: srnty <@t> aol.com <srnty <@t> aol.com>
> Subject: [Histonet] Help With Hemo Fading
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, July 2, 2009, 2:42 PM
>
>
>
> Hello everyone,
>
> ?
>
> We are having problems with short-term hematoxylin fading and loss of
> detail. The pathologist is freaking out! I've seen hemo fade over a long
> period of time but not in a matter of a few months. Slides from one year ago
> are really bad.
>
> ?
>
> I've been out of the business for a number of years and in the interim much
> has changed including reagents. These are GI tract biopsies processed by
> microwave.
>
> ?
>
> Any thoughts at all?
>
> ?
>
> Thanks! Marg
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
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