[Histonet] IHC on frozens

[Rene J Buesa]

Kimberly:
Your question has a two parts answer:
1- it cannot be done because the sections will peel off, but most importantly
2- it is not necessary since HIER was developed to "undo" the cross linkage produced by the NBF fixation, and the tissues used for FS are not fixed.
rené J. 

--- On Thu, 7/2/09, Kimberly Tuttle <ktuttle <@t> umm.edu> wrote:


From: Kimberly Tuttle <ktuttle <@t> umm.edu>
Subject: [Histonet] IHC on frozens
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, July 2, 2009, 12:55 PM


Can you do heat retrieval on frozens?

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 
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