[Histonet] Re: Eosin in alcohol

[Morken, Tim]

The eosin does not affect antibody binding but might interfere with interpretation if they are doing multi-immunostaining on prostates and use a red chromogen. Then the eosin may give an appearance of background. Or maybe they just don't like the color introduced.

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, July 01, 2009 12:41 PM
To: histonet <@t> lists.utsouthwestern.edu; Jennifer Johnson
Subject: [Histonet] Re: Eosin in alcohol

I have never heard of eosin interfering with IHC, as a matter of fact, I did use it regularly and never had a problem with the IHC.
Perhaps there was another cause for the problems with the IHC.
rené J.

--- On Wed, 7/1/09, Jennifer Johnson <jmjohnson34 <@t> hotmail.com> wrote:


From: Jennifer Johnson <jmjohnson34 <@t> hotmail.com>
Subject: [Histonet] Re: Eosin in alcohol
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, July 1, 2009, 1:06 PM



We have used Eosin in the last 95% alcohol on the tissue processor for several years.  I usually add approximately 5 ml to the full jug.  It is a great tool to use for embedding.  However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining.  They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue.  There is no delineations in the color of the blue and I found it to be useless for helping to embed.  I would rather do without anything than use cobalt blue.  I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies.  

Thanks,

Jennifer Johnson, HTL (ASCP) 

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