From tifei <@t> foxmail.com Wed Jul 1 00:32:32 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Jul 1 00:32:59 2009 Subject: [Histonet] RE: mouse brain fixation Attn: Annette Featherstone References: <000601c9f99a$9db64de0$d922e9a0$@callis@bresnan.net> Message-ID: <200907011332271506852@foxmail.com> SSB0cmllZCBaaW5jIGZpeGF0aXZlIEkgbWFkZSBteXNlbGYuDQpEb2VzIG5vdCB3b3JrIGF0IGFs bD8gQW5kLCB0aGUgdGlzc3VlIHF1YWxpdHkgaXMgdmVyeSBiYWQuDQpBbnkgY29tbWVudHM/DQoN Cg0KMjAwOS0wNy0wMSANCg0KDQoNClRGIA0KDQoNCg0Kt6K8/sjLo7ogZ2F5bGUgY2FsbGlzIA0K t6LLzcqxvOSjuiAyMDA5LTA2LTMwICAyMzo1NzozNSANCsrVvP7Iy6O6ICdIaXN0b25ldCcgDQqz rcvNo7ogDQrW98zio7ogW0hpc3RvbmV0XSBSRTogbW91c2UgYnJhaW4gZml4YXRpb24gQXR0bjog QW5uZXR0ZSBGZWF0aGVyc3RvbmUgDQogDQpBbm5ldHRlLCANCg0KWW91IGRpZCBub3Qgc2F5IHdo YXQgaW1tdW5vcyBlLmcuIGFudGlnZW5zIHlvdSBhcmUgdHJ5aW5nIHRvIHN0YWluPyAgIE5vcg0K aG93IHlvdSBhcmUgZG9pbmcgdGhlIGFjdHVhbCBzdGFpbmluZyBtZXRob2Q/ICBNb3JlIGluZm9y bWF0aW9uIHdvdWxkIGhlbHANCnBsZWFzZS4gDQoNCklmIHlvdSBhcmUgdHJ5aW5nIHRvIHN0YWlu 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Subject: [Histonet] signs of good perfusion References: <000001c9f937$9c779550$d566bff0$@raghul@rccltd.in> Message-ID: <200907011337412720376@foxmail.com> SSB0aGluayAiZ29vZCIgZm9yIHBlcmZ1c2lvbi9maXhhdGlvbiBpcyBkaWZmZXJlbnQgdXBvbiBy ZXF1ZXN0LCB0aGUgYW50aWdlbiBpbiBJSEMsIGUuZy4NCkZvciBnb29kIGhpc3RvbG9neSBhbmQg Zml4YXRpdmUtcmVzaXN0ZW50IGFudGlnZW5zLCBwZW9wbGUgdHJ5IHRvIGZpeCB0aGUgdGlzc3Vl IGV4dGVuc2l2ZWx5LCB0byBoYXZlIGdvb2QgdGlzc3VlIHF1YWxpdHkgYW5kIHRoZSBhbmltYWwg Z29lcyBhIHN0aWZmLg0KRm9yIG1pbGQtcmVzaXN0ZW50IGFudGlnZW5zLCB3ZSBuZXZlciBwb3N0 LWZpeCB0aGUgdGlzc3VlIG1vcmUgdGhhbiA2IGhvdXJzIGFmdGVyIGEgMzAgbWluIHBlcmZ1c2lv biwgZm9yIGV4YW1wbGUsIHdoZW4gZXhhbWluZyBIUlAgaW5zaWRlIHRoZSB0aXNzdWUuDQpGb3Ig bWFueSBDRCBtYXJrZXJzLCBhIGJyaWVmIDQlIFBGQSBwZXJmdXNpb24gd2FzIGZvbGxvd2VkIGJ5 IDMwJSBzdXJjb3NlIHBlcmZ1c2lvbi4gVGhlbiB0aGUgYnJhaW4gd2FzIHBvc3QtZGVoeWRyYXRl ZCBpbiAzMCUgc3Vjcm9zZSBhdCA0IEMuDQoNCg0KMjAwOS0wNy0wMSANCg0KDQoNClRGIA0KDQoN 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<200907011339451203913@foxmail.com> Pale liver means the blood has been removed from liver, not necessarily any other tissues of interest. For brain perfusion, many people will close descending atery. The lung filling up of liquid is because you did not insert needle into the main artery. It is small, but can be done with proper sized needle. 2009-07-01 TF ???? Joseph Saby ????? 2009-06-30 07:44:28 ???? Merced M Leiker; Thach, Dzung (NIH/NIAID) [E]; histonet ??? ??? Re: [Histonet] Signs of good perfusion I agree that the pale liver is a great sign.?However, if the lungs fill up fluid comes out the nostrels or mouth, then the needle is probably inserted too far and has gone into the pulmonary vein. I hope this helps! Joe ________________________________ From: Merced M Leiker To: "Thach, Dzung (NIH/NIAID) [E]" ; histonet@lists.utsouthwestern.edu Sent: Monday, June 29, 2009 12:07:04 PM Subject: Re: [Histonet] Signs of good perfusion Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculature....ideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" wrote: > Hi Everyone!! > >??I am perfusing CO2 euthanized 3 weeks old mice with PBS only.?I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump.?Sometimes I see the lungs swelling up and fluid comes > out of mouth.?Occasionally, I see the liver fade to light pink.?Most of > the time the paws become white.?But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused.?I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY?14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Jul 1 00:48:22 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Wed Jul 1 01:19:15 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIEdvb2QgUGVyZnVzaW9uLi4u?= References: , <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH>, <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com>, , <3f4b71f10906301825l26712686m90249c1e50a3ffd3@mail.gmail.com> Message-ID: <200907011348171284002@foxmail.com> let me say like this during the overdose injection, look into the animals' eyes. take their soul away. then the pre-mortem perfusion is humanized. 2009-07-01 TF ???? Chana de Wolf ????? 2009-07-01 09:30:01 ???? Ingles Claire ??? histonet ??? Re: [Histonet] Good Perfusion... Actually, as I explained, in the case of perfusion fixation, you can *and* you should begin perfusion pre-mortem if you wish to measure anything other than ischemic injury. That's pretty much the point. Your own personal feelings aside, it is extremely important that people on this list who are not familiar with standard perfusion protocol do not get the erroneous idea that such procedures are illegal or inhumane. The animal is deeply anesthetized prior to the procedure and death is painless. While not the most aesthetically attractive method of euthanasia, it is simply a thoracotomy followed by exsanguination, which is an AVMA approved method of euthanasia when used in combination with sedation or anesthesia. I am not interested in "flaming," simply in clarifying. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 2:29 PM, Ingles Claire wrote: > Sorry, but it is against my personal ethics (and/or morals?) to do such a > thing. Guess I'll never get to work in research... But then I can't even > pith a frog without squirming. After it's dead though, nothing bothers me. > (OK, maybe eyes) Perhaps I'm just wierd. (oh, wait, I work in a lab...) > Like I always try to explain to the pathology residents who are trying to > cram 3cmx3cmx5mm pieces of breast tissue into a cassette. "Just because you > can, doesn't mean you should." Don't get me wrong, I DON'T volunteer with > PETA, etc. and know animal models are instrumental to many research > programs. But I have a hard time figuring out where gray area turns to > black. > Let the flaming begin. And it's only Tuesday. Boy I am on a roll! > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chana de Wolf > Sent: Tue 6/30/2009 2:37 PM > To: jfish@gladstone.ucsf.edu > Cc: histonet@lists.utsouthwestern.edu; JR R > Subject: Re: [Histonet] Good Perfusion... > > > > Histonetters, > > Perfusion under deep anesthesia is most certainly not unethical NOR > illegal, > and, in fact (as mentioned by Jo Dee), it is necessary for optimal > perfusion > and fixation -- the intracellular ischemic cascade begins immediately upon > circulatory arrest, setting off a chain of events highly detrimental to > subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" > phenomenon, effectively guaranteeing that you are not perfusing all tissues > adequately at all! It is therefore *most* beneficial to begin perfusion > with > a beating heart, and every perfusion protocol I have ever worked under > requires it (especially if the tissues are to be used for EM). > > I perform around 5-10 perfusions per week *specifically* to study the > effects of ischemia on reperfusion and neural ultrastructure. > > Of course, you should check with your institution's particular rules and > regulations, but perfusion begun under anesthesia is scientifically > justified for the reasons mentioned above and should therefore be > relatively > easy to have approved by your IACUC. > > Sincerely, > > Chana de Wolf > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Jul 1 00:45:56 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Wed Jul 1 01:20:36 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIEdvb2QgUGVyZnVzaW9uLi4u?= References: , <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH>, <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> Message-ID: <200907011345508158713@foxmail.com> I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP". It is hard to judge whether the animal can recover. But we know not because it is an overdose injection. Then, when you write papers up, you never use "Deep anesthesia", you use "sacrifice". That is some back rules in writing papers, but it make some European reviewers better. To have good fixation on inschemic area, post-fixation at room temperature with changes of fixative every 24-48 hours is required. 2009-07-01 TF ???? Chana de Wolf ????? 2009-07-01 03:42:30 ???? jfish ??? histonet; JR R ??? Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish wrote: > Dear Jerry and histonetters, > > I don't believe this is unethical or illegal. It is written into our IACUC > approved protocols as follows: > > Chemical Method of Euthanasia: "Perfusion under general anesthesia, > Avertin > or Halothane induced. Bilateral thoracotomy." > > Could it only be forbidden by your facility? > > I wonder if other facilities have rules against such things. But we insist > that our investigators use perfusion under anesthsia because the deeper > tissues are much better fixed and blood removal is more thorough and > complete by this method. > > Jo Dee > > > ~~Jo Dee Fish~~ > Senior Research Technologist > The J. David Gladstone Institutes > Co-manager Histology and Microscopy Core > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R > Sent: Tuesday, June 30, 2009 10:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Good Perfusion... > > > No, I wouldn't nick the atrium. > > Instead, cut the femoral artery at the groin. > > And of course, you cannot perfuse a living, anesthetized animal. That > would > be unethical and also illegal. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Insert movie times and more without leaving HotmailR. > > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori > al_QuickAdd_062009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jul 1 08:14:09 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jul 1 08:14:07 2009 Subject: [Histonet] Resubmitting the question regarding 2011 CA Certification Message-ID: <5810C70B-1610-4340-83C3-49912B08B9F8@yahoo.com> Good Morning, I have not received any replies to my proposed question regarding the following question. I don't know if the histotech's that have the answers were on vacation, but I sure would like any information. Below is the original e-mail: I need your assistance on a on-going question which has been kicked around since 2005. I tried to look this up in the Histonet Archives, but was unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011. It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants. Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL From hstoqwn <@t> gmail.com Wed Jul 1 08:45:05 2009 From: hstoqwn <@t> gmail.com (Beth Couch) Date: Wed Jul 1 08:45:10 2009 Subject: [Histonet] eosin in processing alcohols Message-ID: <4f04a7f30907010645j50dd45aale256a1019de39fc0@mail.gmail.com> Hi Histonetters - do any of you use easin in the processing alcohols in order to better visualize small bipsies and if so how much eosin to alcohol do you use and in which alcohol? Thank you in advance for your help. From leiker <@t> buffalo.edu Wed Jul 1 10:25:41 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Jul 1 10:25:45 2009 Subject: [Histonet] Good Perfusion... In-Reply-To: <200907011345508158713@foxmail.com> References: , <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH> , <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> <200907011345508158713@foxmail.com> Message-ID: <84A483D04878D41C3D1CB694@CDYwxp1931.ad.med.buffalo.edu> Hey everyone...just thought I'd add an "official" word from the authorities in this debate...as I'd been taught or somehow led to believe otherwise: :-) ------------ Forwarded Message ------------ Date: Wednesday, July 01, 2009 11:19 AM -0400 From: IACUC To: Merced M Leiker Subject: RE: general question: pre-mortem perfusion Perfusion under deep anesthesia is an acceptable method of euthanasia. -----Original Message----- From: Merced M Leiker [mailto:leiker@buffalo.edu] Sent: Wednesday, July 01, 2009 9:50 AM To: IACUC Subject: general question: pre-mortem perfusion Hi there, I am in a debate about pre-mortem perfusion fixation of an animal (a small rodent) while it is under deep anesthesia. Would this be allowed by our IACUC guidelines or not? Or would the animal rather have to be perfused post-mortem? Thank you for your advice. Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From gayle.callis <@t> bresnan.net Wed Jul 1 11:01:50 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Jul 1 11:02:14 2009 Subject: [Histonet] Good Perfusion... In-Reply-To: <200907011345508158713@foxmail.com> References: , <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH>, <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> <200907011345508158713@foxmail.com> Message-ID: <002c01c9fa65$3faec8f0$bf0c5ad0$@callis@bresnan.net> It is also considered incorrect to use the word "sacrifice", implying some type of ceremony, religious or otherwise. Animal rights fanatics have a hey day with this word. Euthanasia is more correct. Gayle M. Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TF Sent: Tuesday, June 30, 2009 11:46 PM To: Chana de Wolf; jfish Cc: histonet; JR R Subject: Re: Re: [Histonet] Good Perfusion... I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP". It is hard to judge whether the animal can recover. But we know not because it is an overdose injection. Then, when you write papers up, you never use "Deep anesthesia", you use "sacrifice". That is some back rules in writing papers, but it make some European reviewers better. To have good fixation on inschemic area, post-fixation at room temperature with changes of fixative every 24-48 hours is required. 2009-07-01 TF ???? Chana de Wolf ????? 2009-07-01 03:42:30 ???? jfish ??? histonet; JR R ??? Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish wrote: > Dear Jerry and histonetters, > > I don't believe this is unethical or illegal. It is written into our > IACUC approved protocols as follows: > > Chemical Method of Euthanasia: "Perfusion under general anesthesia, > Avertin or Halothane induced. Bilateral thoracotomy." > > Could it only be forbidden by your facility? > > I wonder if other facilities have rules against such things. But we > insist that our investigators use perfusion under anesthsia because > the deeper tissues are much better fixed and blood removal is more > thorough and complete by this method. > > Jo Dee > > > ~~Jo Dee Fish~~ > Senior Research Technologist > The J. David Gladstone Institutes > Co-manager Histology and Microscopy Core > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R > Sent: Tuesday, June 30, 2009 10:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Good Perfusion... > > > No, I wouldn't nick the atrium. > > Instead, cut the femoral artery at the groin. > > And of course, you cannot perfuse a living, anesthetized animal. That > would be unethical and also illegal. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Insert movie times and more without leaving HotmailR. > > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_ > Tutori > al_QuickAdd_062009_______________________________________________ ://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tuto > ri%0Aal_QuickAdd_062009_______________________________________________ > > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Jul 1 11:08:45 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Jul 1 11:09:08 2009 Subject: [Histonet] RE: mouse brain fixation Attn: Annette Featherstone In-Reply-To: <200907011332271506852@foxmail.com> References: <000601c9f99a$9db64de0$d922e9a0$@callis@bresnan.net> <200907011332271506852@foxmail.com> Message-ID: <002d01c9fa66$3738fe60$a5aafb20$@callis@bresnan.net> When you say zinc fixative, which one are you talking about? Becksteads Zinc TRIS buffer made up with zinc salts and formalin free? Or zinc formalin. The Becksteads zinc salt fixative does work, as cited in = several papers in the literature, and we tried it but preferred to use frozen sections, just faster overall. And if you overexpose the tissues to dehydrant, clearing agents and heat of paraffin, the sectioning can be = worse with the Beckstead fixative. It makes the tissue dry and crunchy. IF = you do not fix the tissues properly and totally, then the alcohols used in processing will finish the fixation, and that isn't going to contribute = to good sectioning either. How you make it up is critical, and should be = done according to the original publication or Nitta et al paper. =20 =20 =20 Gayle M. Callis =20 =20 From: TF [mailto:tifei@foxmail.com]=20 Sent: Tuesday, June 30, 2009 11:33 PM To: gayle callis; 'Histonet' Subject: Re: [Histonet] RE: mouse brain fixation Attn: Annette = Featherstone =20 I tried Zinc fixative I made myself. Does not work at all? And, the tissue quality is very bad. Any comments? =20 =20 2009-07-01=20 _____ =20 TF=20 _____ =20 =B7=A2=BC=FE=C8=CB=A3=BA gayle callis=20 =B7=A2=CB=CD=CA=B1=BC=E4=A3=BA 2009-06-30 23:57:35=20 =CA=D5=BC=FE=C8=CB=A3=BA 'Histonet'=20 =B3=AD=CB=CD=A3=BA=20 =D6=F7=CC=E2=A3=BA [Histonet] RE: mouse brain fixation Attn: Annette = Featherstone=20 Annette,=20 =20 You did not say what immunos e.g. antigens you are trying to stain? = Nor how you are doing the actual staining method? More information would = help please.=20 =20 If you are trying to stain for murine CD markers or some other cellular antigens, there are not many that work after FFPE. If you need to know = this information, go to SEROTEC and look at murine CD or other cellular = markers, and see if the antibody will work with FFPE paraffin applications. BD Bioscience also has applications that work, tested, reported, IHC or = frozen sections and found in their Technical Data Sheets for any given = antibody. =20 There are some other fixatives to try for CD and other cellular markers = - SEROTEC often refers to PLP (paraformaldehyde-lysine-periodate with = recipe found on web through IHCWorld or Immunoportal, Google the keywords) = while BD Bioscience refers to Becksteads Formalin free Zn TRIS buffer fixative. = If you need the recipe for that, I will be happy to send. Otherwise BD Bioscience would be happy to sell that to you but it is cheaper and easy = to make up in the lab.=20 =20 Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT=20 =20 =20 =20 =20 =20 You wrote: =20 =20 We have been working with mouse brains that were fixed in 10% formalin = for one month. We are currently getting "no" staining with our immunos. Can = this really be the problem in light of antigen retrieval methods? =20 =20 =20 Annette Featherstone =20 =20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Wed Jul 1 11:34:27 2009 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Wed Jul 1 11:34:31 2009 Subject: [Histonet] CD 102 (ICAM2) Message-ID: <00E9EC516E0CEB4A9ED53E7FAD19DE0902354E38@nrccenexb1.nrc.ca> Hi, I would like to get some help on labelling CD102 by an immunohistochemical method on mouse heart after it has gone through Langendorff perfusion with ice-cold Krebs-Henseleit buffer during an experiment. We have purchased BD Pharmingen's 'Purified Rat-Anti-Mouse CD102' and 'Anti-Rat Ig HRP detection kit'. Will be using normal goat serum for blocking the non-specific binding.These reagents have been tested in frozen sections only by BD. Since I have not done that many immunohistochemical assays, I would like to get answers to the following from the experts: 1. Has anybody done this on paraffin embedded tissue sections using the same reagents? 2. If using frozen, how do you prepare the tissue? Do you fix the tissue before freezing or soon after it has been sectioned? Do you use 4% paraformaldehyde or of a lesser concentration? Do you cryo-protect using sucrose in PBS? If so, sucrose is used at what percentage? 3. What's the thickness range of the tissue section recommended for the frozen tissue? 4. What kind of dilutions I need to include for the primary antibody and for the secondary antibody for my initial trial run during optimization? 5. What are the incubation times and temperature for the primary and secondary antibodies? 6. Which tissue type would be the best positive control to use in the assay? Any kind of help with this protocol would be greatly appreciated! Thank you so much! Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca From tifei <@t> foxmail.com Wed Jul 1 11:49:07 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Jul 1 11:49:39 2009 Subject: [Histonet] RE: mouse brain fixation Attn: Annette Featherstone References: <000601c9f99a$9db64de0$d922e9a0$@callis@bresnan.net>, <200907011332271506852@foxmail.com>, <002d01c9fa66$3738fe60$a5aafb20$@callis@bresnan.net> Message-ID: <200907020049017454522@foxmail.com> aEksIGkgQU0gdXNpbmcgZm9ybWFsaW4tZnJlZSBaaW5jZSBmaXhhdGl2ZSBtYWRlIGZyb20gWmlu YyBzYWx0ICYgVHJpcyBidWZmZXIuDQpUaGVuIEkgbWFkZSBmcm96ZW4gc2VjdGlvbnMgYWZ0ZXIg ZGVoeWRyYXRpb24gaW4gMzAlIHN1Y3Jvc2UuLi4NCk5vIENEMzErIHN0YWluaW5nLi4uDQoNCkkg YWxzbyB0cmllZCBzaG9jayBmcm96ZW4sIGFjZXRvbmUgZml4ZWQgdGlzc3VlLCB0aGUgYW50aWJv ZHkgd29ya3MgaW4gdGhpcyBjb25kaXRpb24uDQoNCg0KMjAwOS0wNy0wMiANCg0KDQoNClRGIA0K DQoNCg0Kt6K8/sjLo7ogZ2F5bGUgY2FsbGlzIA0Kt6LLzcqxvOSjuiAyMDA5LTA3LTAyICAwMDow 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IGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0 c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo= From rosenfeldtek <@t> hotmail.com Wed Jul 1 11:54:30 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Wed Jul 1 11:54:36 2009 Subject: [Histonet] Good Perfusion..."Sacrifice" vs "anesthesia" In-Reply-To: <200907011345508158713@foxmail.com> References: , <47D48B82BA784DCDB18A4AA9AE0FD080@JFISH>, <3f4b71f10906301237s5cc3e898k1db8c10339b02f2b@mail.gmail.com> <200907011345508158713@foxmail.com> Message-ID: Maybe that's what was bugging me before. I overdose mice on ketamine-xylazine. They go into respiratory arrest, and have no toe-pinch reflex, but the heart is usually beating when I perfuse them. I suppose technically, they are "anesthetized" and could revover if I put them on a ventilator or something. But I prefer to think of it and report it as "sacrificed" rather than "under deep anesthesia." Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Wed, 1 Jul 2009 13:45:56 +0800 From: tifei@foxmail.com To: chana.de.wolf@gmail.com; jfish@gladstone.ucsf.edu CC: histonet@lists.utsouthwestern.edu; rosenfeldtek@hotmail.com Subject: Re: Re: [Histonet] Good Perfusion... I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP". It is hard to judge whether the animal can recover. But we know not because it is an overdose injection. Then, when you write papers up, you never use "Deep anesthesia", you use "sacrifice". That is some back rules in writing papers, but it make some European reviewers better. To have good fixation on inschemic area, post-fixation at room temperature with changes of fixative every 24-48 hours is required. 2009-07-01 TF 发件人： Chana de Wolf 发送时间： 2009-07-01 03:42:30 收件人： jfish 抄送： histonet; JR R 主题： Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highly detrimental to subsequent perfusion. Indeed, ischemia quickly leads to the "no-reflow" phenomenon, effectively guaranteeing that you are not perfusing all tissues adequately at all! It is therefore *most* beneficial to begin perfusion with a beating heart, and every perfusion protocol I have ever worked under requires it (especially if the tissues are to be used for EM). I perform around 5-10 perfusions per week *specifically* to study the effects of ischemia on reperfusion and neural ultrastructure. Of course, you should check with your institution's particular rules and regulations, but perfusion begun under anesthesia is scientifically justified for the reasons mentioned above and should therefore be relatively easy to have approved by your IACUC. Sincerely, Chana de Wolf On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish wrote: > Dear Jerry and histonetters, > > I don't believe this is unethical or illegal. It is written into our IACUC > approved protocols as follows: > > Chemical Method of Euthanasia: "Perfusion under general anesthesia, > Avertin > or Halothane induced. Bilateral thoracotomy." > > Could it only be forbidden by your facility? > > I wonder if other facilities have rules against such things. But we insist > that our investigators use perfusion under anesthsia because the deeper > tissues are much better fixed and blood removal is more thorough and > complete by this method. > > Jo Dee > > > ~~Jo Dee Fish~~ > Senior Research Technologist > The J. David Gladstone Institutes > Co-manager Histology and Microscopy Core > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R > Sent: Tuesday, June 30, 2009 10:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Good Perfusion... > > > No, I wouldn't nick the atrium. > > Instead, cut the femoral artery at the groin. > > And of course, you cannot perfuse a living, anesthetized animal. That > would > be unethical and also illegal. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Insert movie times and more without leaving HotmailR. > > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori > al_QuickAdd_062009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Lauren found her dream laptop. Find the PC that’s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290From jmjohnson34 <@t> hotmail.com Wed Jul 1 12:06:41 2009 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Wed Jul 1 12:06:45 2009 Subject: [Histonet] Re: Eosin in alcohol Message-ID: We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009 From DKBoyd <@t> chs.net Wed Jul 1 12:43:14 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jul 1 12:41:39 2009 Subject: [Histonet] Re: Eosin in alcohol In-Reply-To: Message-ID: We use eosin on the gross bench. We keep a bottle of concentrated Eosin and Hematoxylin on the bench with an eyedropper. The eosin is for endoscopic biopsies and the hematoxylin is for prostate biopsies. Neither has never interfered with IHC staining. Debbie M. Boyd, HT(ASCP),Chief Histologist, Southside Regional Medical Center, 200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F: 804-765-5582, dkboyd@chs.net Jennifer Johnson Sent by: histonet-bounces@lists.utsouthwestern.edu 07/01/2009 01:09 PM To cc Subject [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From pruegg <@t> ihctech.net Wed Jul 1 12:42:09 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jul 1 12:42:15 2009 Subject: [Histonet] slide cabinets Message-ID: <6D9E673E92594D92A8A4724CCD6D4F78@Patsyoffice> Folks, I inherited some nice new metal cabinets (Sakura Tissue Tek) brand, for holding glass microscope slides. The drawers are missing the inserts that make so you can line up two rows of slides side by side, I am wondering where I could buy those. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From lblazek <@t> digestivespecialists.com Wed Jul 1 12:47:29 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 1 12:45:44 2009 Subject: [Histonet] Re: Eosin in alcohol In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39088C7B3141@IBMB7Exchange.digestivespecialists.com> We use about the same amount or eosin in the first 100% alcohol and also do immunos on the biopsies and have no problem. We do all GI biopsies though not prostate. I'm not sure why the eosin would interfere with the immuno staining since it disappears during the hydration and retrieval process of staining immunos. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Wednesday, July 01, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Wed Jul 1 12:51:34 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Jul 1 12:51:47 2009 Subject: [Histonet] Re: Eosin in alcohol In-Reply-To: References: Message-ID: Jennifer, is it possible that the lab asking you to use cobalt blue is using a fluorescence technique on the prostates? We've used eosin for years and not seen any impact on IHC staining for light microscopy. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Wednesday, July 01, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jul 1 13:39:53 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 1 13:39:58 2009 Subject: [Histonet] Re: Eosin in alcohol In-Reply-To: References: Message-ID: <5b6eb13e0907011139l68d68ec7r70604273b81e3000@mail.gmail.com> I think they are probably getting weak unwanted staining with P504s/AMACR antibody on benign glands on their PIN4s. Since this antibody is usually pink/red, it sounds like someone might have decided that the unwanted color came from eosin in the processor, but this would be the wrong conclusion. Eosin washes out. Mark Tarango HT(ASCP)QIHC On Wed, Jul 1, 2009 at 10:06 AM, Jennifer Johnson wrote: > > We have used Eosin in the last 95% alcohol on the tissue processor for > several years. I usually add approximately 5 ml to the full jug. It is a > great tool to use for embedding. However, we received a letter from the lab > that we send our prostate biopsies to saying that it was undesirable because > it interfered with their immuno staining. They sent us some cobalt blue to > use in the place of eosin along with mixing instructions and the whole batch > of tissues came out such a dark blue. There is no delineations in the color > of the blue and I found it to be useless for helping to embed. I would > rather do without anything than use cobalt blue. I guess the point of my > rambling is, Eosin is a wonderful tool to use unless you are doing immunos > on prostate biopsies. > > Thanks, > > Jennifer Johnson, HTL (ASCP) > > _________________________________________________________________ > Hotmail? has ever-growing storage! Don?t worry about storage limits. > > http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CThornton <@t> dahlchase.com Wed Jul 1 13:44:25 2009 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Jul 1 13:44:31 2009 Subject: [Histonet] Ventana Symphony Message-ID: Is anyone out there using the Symphony stainer by Ventana? Pros, cons, issues, service? What have your experiences been? Thanks! Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From shshaw <@t> WPI.EDU Wed Jul 1 14:34:26 2009 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Wed Jul 1 14:34:34 2009 Subject: [Histonet] EDC Fixation Message-ID: Hi Everyone, I'm looking to see if anyone has used EDC fixation with formalin, if so do you EDC fix first followed by formalin or formalin fix followed by EDC. I would love to have some feedback on this. Regards, Sharon _______________________________________________________________________________ Sharon Shaw Worcester Polytechnic Institute Laboratory and Histology Manager Department of Biomedical Engineering 100 Institute Road Worcester,MA 01609 508-831-4125 shshaw@wpi.edu From rjbuesa <@t> yahoo.com Wed Jul 1 14:41:00 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 1 14:41:05 2009 Subject: [Histonet] Re: Eosin in alcohol Message-ID: <33309.70652.qm@web65702.mail.ac4.yahoo.com> I have never heard of eosin interfering with IHC, as a matter of fact, I did use it regularly and never had a problem with the IHC. Perhaps there was another cause for the problems with the IHC. ren? J. --- On Wed, 7/1/09, Jennifer Johnson wrote: From: Jennifer Johnson Subject: [Histonet] Re: Eosin in alcohol To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 1, 2009, 1:06 PM We have used Eosin in the last 95% alcohol on the tissue processor for several years.? I usually add approximately 5 ml to the full jug.? It is a great tool to use for embedding.? However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining.? They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue.? There is no delineations in the color of the blue and I found it to be useless for helping to embed.? I would rather do without anything than use cobalt blue.? I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies.? Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail? has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Jul 1 14:53:34 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Jul 1 14:53:43 2009 Subject: [Histonet] Re: Eosin in alcohol In-Reply-To: <33309.70652.qm@web65702.mail.ac4.yahoo.com> References: <33309.70652.qm@web65702.mail.ac4.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C21CAB0A0@EXMBMCB15.ucsfmedicalcenter.org> The eosin does not affect antibody binding but might interfere with interpretation if they are doing multi-immunostaining on prostates and use a red chromogen. Then the eosin may give an appearance of background. Or maybe they just don't like the color introduced. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 01, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu; Jennifer Johnson Subject: [Histonet] Re: Eosin in alcohol I have never heard of eosin interfering with IHC, as a matter of fact, I did use it regularly and never had a problem with the IHC. Perhaps there was another cause for the problems with the IHC. ren? J. --- On Wed, 7/1/09, Jennifer Johnson wrote: From: Jennifer Johnson Subject: [Histonet] Re: Eosin in alcohol To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 1, 2009, 1:06 PM We have used Eosin in the last 95% alcohol on the tissue processor for several years.? I usually add approximately 5 ml to the full jug.? It is a great tool to use for embedding.? However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining.? They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue.? There is no delineations in the color of the blue and I found it to be useless for helping to embed.? I would rather do without anything than use cobalt blue.? I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies.? Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail? has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Jul 1 16:32:49 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Jul 1 16:33:41 2009 Subject: [Histonet] Re: Eosin in alcohol In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39088C7B3141@IBMB7Exchange.digestivespecialists.com> References: , <5A2BD13465E061429D6455C8D6B40E39088C7B3141@IBMB7Exchange.digestivespecialists.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D362672CA@LRGHEXVS1.practice.lrgh.org> Are the prostate biopsies, fixed in formalin or Bouins? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda [lblazek@digestivespecialists.com] Sent: Wednesday, July 01, 2009 1:47 PM To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Eosin in alcohol We use about the same amount or eosin in the first 100% alcohol and also do immunos on the biopsies and have no problem. We do all GI biopsies though not prostate. I'm not sure why the eosin would interfere with the immuno staining since it disappears during the hydration and retrieval process of staining immunos. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Wednesday, July 01, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Charlene.Henry <@t> STJUDE.ORG Wed Jul 1 16:41:45 2009 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Jul 1 16:41:49 2009 Subject: [Histonet] Ventana Symphony. . In-Reply-To: References: Message-ID: <03E1F5968F60C5448635D49D38B283ED154C94BE3D@SJMEMXMBS11.stjude.sjcrh.local> We have a Symphony and my techs absolutely love this instrument. It is very easy to use and the stain/reagent maintenance is so simple. No more changing and rotating solutions. The staining quality is excellent and Ventana's customer service is great. I would definitely recommend the Symphony. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Manager Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 phone 901-595-3191 fax 901-595-3100 www.charlene.henry@stjude.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, July 01, 2009 1:44 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana Symphony. . Is anyone out there using the Symphony stainer by Ventana? Pros, cons, issues, service? What have your experiences been? Thanks! Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Email Disclaimer: www.stjude.org/emaildisclaimer From marktarango <@t> gmail.com Wed Jul 1 16:50:30 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 1 16:50:34 2009 Subject: [Histonet] Resubmitting the question regarding 2011 CA Certification In-Reply-To: <5810C70B-1610-4340-83C3-49912B08B9F8@yahoo.com> References: <5810C70B-1610-4340-83C3-49912B08B9F8@yahoo.com> Message-ID: <5b6eb13e0907011450l735b09a9j44369153eaa082a8@mail.gmail.com> Hi Akemi, The only time I remember anyone ever saying California histotechs had to be certified was when you posted something a while back. You didn't give any information about from where this information came. Where'd you hear this rumor? Mark Tarango On Wed, Jul 1, 2009 at 6:14 AM, Akemi Allison-Tacha wrote: > Good Morning, > > I have not received any replies to my proposed question regarding the > following question. I don't know if the histotech's that have the answers > were on vacation, but I sure would like any information. Below is the > original e-mail: > > I need your assistance on a on-going question which has been kicked around > since 2005. I tried to look this up in the Histonet Archives, but was > unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my > understanding that Governor Arnold Schwarzenegger put a mandate on all > laboratory staff, including histologists, that they were required to be > certified by 2011. It was further my understanding that un-certified > histology staff were only going to be able to work as histology assistants. > Could I get some clarification on this matter. > > Thanks, > Akemi Allison-Tacha BS, HT/HTL > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From thecitan <@t> yahoo.com Wed Jul 1 16:57:09 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Wed Jul 1 16:56:56 2009 Subject: [Histonet] Resubmitting the question regarding 2011 CA Certification Message-ID: <432741976-1246485411-cardhu_decombobulator_blackberry.rim.net-1854337317-@bxe1123.bisx.prod.on.blackberry> This rumor has gone as far as my classroom. Our teacher has mentioned it for many years as a "possibility". I have never seen or heard of any official source. Sent from my Verizon Wireless BlackBerry From marktarango <@t> gmail.com Wed Jul 1 16:58:56 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 1 16:59:00 2009 Subject: [Histonet] Resubmitting the question regarding 2011 CA Certification In-Reply-To: <432741976-1246485411-cardhu_decombobulator_blackberry.rim.net-1854337317-@bxe1123.bisx.prod.on.blackberry> References: <432741976-1246485411-cardhu_decombobulator_blackberry.rim.net-1854337317-@bxe1123.bisx.prod.on.blackberry> Message-ID: <5b6eb13e0907011458g4da046c2k7c69e130b3811ab9@mail.gmail.com> I think I remember someone saying once that this was part of a failed piece of legislation. I thought I'd read it on histonet, but I couldn't find an old post saying that. Mark Tarango On Wed, Jul 1, 2009 at 2:57 PM, wrote: > This rumor has gone as far as my classroom. Our teacher has mentioned it > for many years as a "possibility". I have never seen or heard of any > official source. > Sent from my Verizon Wireless BlackBerry > > From rsrichmond <@t> gmail.com Wed Jul 1 18:03:01 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jul 1 18:03:05 2009 Subject: [Histonet] Re: Good Perfusion..."Sacrifice" vs "anesthesia" Message-ID: That sound you hear is George Orwell turning over in his grave. Bob Richmond Samurai Pathologist Knoxville TN From wdesalvo.cac <@t> hotmail.com Wed Jul 1 18:09:03 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jul 1 18:09:07 2009 Subject: [Histonet] Ventana Symphony. . In-Reply-To: <03E1F5968F60C5448635D49D38B283ED154C94BE3D@SJMEMXMBS11.stjude.sjcrh.local> References: <03E1F5968F60C5448635D49D38B283ED154C94BE3D@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: I have heard similar responses to yours and I think there is agreement on the quality of the instrument, but what are you paying per slide and how did you justify the cost? In your cost justification, were you able to convert to dollars any of the quality improvements and can you share that calculation? William DeSalvo, B.S., HTL(ASCP) > From: Charlene.Henry@STJUDE.ORG > To: CThornton@dahlchase.com; histonet@lists.utsouthwestern.edu > Date: Wed, 1 Jul 2009 16:41:45 -0500 > Subject: RE: [Histonet] Ventana Symphony. . > CC: > > We have a Symphony and my techs absolutely love this instrument. It is very easy to use and the stain/reagent maintenance is so simple. No more changing and rotating solutions. The staining quality is excellent and Ventana's customer service is great. I would definitely recommend the Symphony. > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Manager > Department of Pathology > St. Jude Children's Research Hospital > 262 Danny Thomas Place > Memphis, TN 38105 > phone 901-595-3191 > fax 901-595-3100 > www.charlene.henry@stjude.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton > Sent: Wednesday, July 01, 2009 1:44 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Ventana Symphony. . > > Is anyone out there using the Symphony stainer by Ventana? Pros, cons, issues, service? What have your experiences been? > > Thanks! > > Clare J. Thornton, HTL(ASCP) > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Email Disclaimer: www.stjude.org/emaildisclaimer > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live? SkyDrive?: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_SD_25GB_062009 From annigyg <@t> gmail.com Thu Jul 2 04:13:50 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Jul 2 04:13:56 2009 Subject: [Histonet] eosin in processing alcohols In-Reply-To: <4f04a7f30907010645j50dd45aale256a1019de39fc0@mail.gmail.com> References: <4f04a7f30907010645j50dd45aale256a1019de39fc0@mail.gmail.com> Message-ID: we put phloxine in the formalin - we find it a better option than eosin 2009/7/1 Beth Couch > Hi Histonetters - do any of you use easin in the processing alcohols in > order to better visualize small bipsies and if so how much eosin to alcohol > do you use and in which alcohol? > Thank you in advance for your help. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From manav.mehta <@t> charite.de Thu Jul 2 07:59:08 2009 From: manav.mehta <@t> charite.de (Manav Mehta) Date: Thu Jul 2 07:59:29 2009 Subject: [Histonet] osteoids Message-ID: <000101c9fb14$e2eb5100$47ac2a8d@biomechanik.chi.charite.de> Dear Histonet'ers, I am trying to stain for Osteoids in a fracture callus of rat femurs. I have MMA and Paraffin sections (4-5 um) / blocks already made. However, I am not sure what stain I should use. Peers have suggested that a Movat pentachrome should work, while some have suggested Goldner, and silver staining. I am not sure how to proceed at the moment. I would appreciate any suggestions with possibly a protocol on the staining for osteoids. It would also help if you provided references on this stain or details on the stain. Life experiences while working with these stains are also welcome. The Goal is to characterize the osteoids in the callus (surface, thickness, osteoblast numbers). Possibly relate them to osteoblastic and -clastic activity. Thanks, Manav Mehta From ratliffjack <@t> hotmail.com Thu Jul 2 08:19:20 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jul 2 08:19:34 2009 Subject: [Histonet] osteoids In-Reply-To: <000101c9fb14$e2eb5100$47ac2a8d@biomechanik.chi.charite.de> References: <000101c9fb14$e2eb5100$47ac2a8d@biomechanik.chi.charite.de> Message-ID: My recommendation to you is to focus on your undemineralized bone sections embedded in MMA. These sections will provide you with the most information. If you do a Goldners stain you can very accurately measure BV/TV and clearly quantitate osteoid volume. Bone will stain green from the SF yellowish staining and osteoid red with the acid fuchsin/ponceau. I feel the only limitation with this stain is clearly assessing your bone cells. If you stain with a Von Kossa/MacNeal's tetrachrome you can achieve the above (bone = black, osteoid = greyish-green) and you can also clearly assess osteoblast (blue) activity. This stain also helps to identify osteoclast presence but hard to accurately determine activity without a TRAP stain follow up. Write me back with any additional questions you may have and when I get to the lab I will forward you a couple of protocols and additional information! Best, Jack Sent from my iPhone On Jul 2, 2009, at 7:59 AM, "Manav Mehta" wrote: > Dear Histonet'ers, > I am trying to stain for Osteoids in a fracture callus of rat > femurs. I > have MMA and Paraffin sections (4-5 um) / blocks already made. > However, > I am not sure what stain I should use. Peers have suggested that a > Movat > pentachrome should work, while some have suggested Goldner, and silver > staining. I am not sure how to proceed at the moment. I would > appreciate > any suggestions with possibly a protocol on the staining for osteoids. > It would also help if you provided references on this stain or details > on the stain. Life experiences while working with these stains are > also > welcome. > > The Goal is to characterize the osteoids in the callus (surface, > thickness, osteoblast numbers). Possibly relate them to osteoblastic > and > -clastic activity. > > Thanks, > Manav Mehta > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From alyssa <@t> alliedsearchpartners.com Thu Jul 2 09:07:02 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Jul 2 09:07:06 2009 Subject: [Histonet] Management Position Available In WA Message-ID: Allied Search Partners has an opening for a Histology Department Manager within a high volume veterinary lab. The position is located in the *Seattle **, **WA** area*. In order to be considered for this position please submit your resume to: Alyssa@alliedsearchpartners.com, and one of our recruiters will give you a call for an initial prescreen phone call, after reviewing your resume. Thank you! *Position:* Histology Department Manager *Reports to:* Medical Director *Qualifications:* - HT certification - 5 years experience in a histology lab - Experience needs to include managing routine histology procedures such as grossing, slide prep, special stains, IHC processing *Responsibilities:* - Oversee the general functions of the histology lab, including hiring, training, evaluating employees, equipment maintenance, assuring compliance with policies and procedures, and maintaining a safe working environment. - Working closely with the Anatomic Pathologists to manage the day to day operations, as well as make recommendations for improving the histology lab. -- Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From Timothy.Morken <@t> ucsfmedctr.org Thu Jul 2 11:53:05 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Jul 2 11:53:15 2009 Subject: [Histonet] Resubmitting the question regarding 2011 CA Certification In-Reply-To: <5810C70B-1610-4340-83C3-49912B08B9F8@yahoo.com> References: <5810C70B-1610-4340-83C3-49912B08B9F8@yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C21D6612B@EXMBMCB15.ucsfmedicalcenter.org> Akemi, here is an answer to your question about California histotech certification / licensure. PA's have to be certified, but not licensed. There are no requirements for histotechs. ________________________________________ From: Simoes, Jennifer (CDPH-LGA) [mailto:Jennifer.Simoes@cdph.ca.gov] Sent: Thursday, July 02, 2009 9:41 AM To: Morken, Tim Subject: RE: Licensing Tim, Per our Laboratory Field Services Program, AB 2156 (Niello, Chapter 319, Statutes of 2006) was signed into law in 2006. This bill specified that a Pathologist's assistant had to be certified by a specified board (Business and Professions Code Section 1269.3). There is no requirement for a state license. The bill also indicated the duties that could be performed by a histologic technician and a histotechnologist, but again, no license is required. Please let me know if you need anything further. Thanks, Jennifer Simoes, Assistant Deputy Director Legislative and Governmental Affairs Department of Public Health State of California ________________________________________ From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, July 01, 2009 3:20 PM To: Simoes, Jennifer (CDPH-LGA) Subject: Licensing Hi, Can you tell me if there has been any previous or recent proposed legislation to require a license for Histotechnician or Histotechnologist at clinical laboratories? Thanks! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, July 01, 2009 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Resubmitting the question regarding 2011 CA Certification Good Morning, I have not received any replies to my proposed question regarding the following question. I don't know if the histotech's that have the answers were on vacation, but I sure would like any information. Below is the original e-mail: I need your assistance on a on-going question which has been kicked around since 2005. I tried to look this up in the Histonet Archives, but was unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011. It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants. Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Thu Jul 2 11:55:47 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Thu Jul 2 11:57:10 2009 Subject: [Histonet] IHC on frozens Message-ID: <4A4CAE53.90CE.001A.3@umm.edu> Can you do heat retrieval on frozens? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From ktuttle <@t> umm.edu Thu Jul 2 12:21:39 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Thu Jul 2 12:23:01 2009 Subject: [Histonet] IHC on frozens In-Reply-To: <4A4CAE53.90CE.001A.3@umm.edu> References: <4A4CAE53.90CE.001A.3@umm.edu> Message-ID: <4A4CB463.90CE.001A.3@umm.edu> Disregard this question! Duh (I should have stayed in bed today) Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 7/2/2009 12:55:47 pm >>> Can you do heat retrieval on frozens? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From tifei <@t> foxmail.com Thu Jul 2 12:26:53 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Jul 2 12:27:17 2009 Subject: [Histonet] IHC on frozens References: <4A4CAE53.90CE.001A.3@umm.edu> Message-ID: <200907030126486492859@foxmail.com> WWVzLCBvbmUgcGFwZXIgcHVibGlzaGVkIG9uIEogSGlzdG9jaGVtIEN5dG9jaGVtLCAyMDA1IG9y IDIwMDYsIGJ5IGEgSmFwYW4gZ3JvdXAuDQoNCg0KMjAwOS0wNy0wMyANCg0KDQoNClRGIA0KDQoN Cg0Kt6K8/sjLo7ogS2ltYmVybHkgVHV0dGxlIA0Kt6LLzcqxvOSjuiAyMDA5LTA3LTAzICAwMTow MToxOCANCsrVvP7Iy6O6IGhpc3RvbmV0IA0Ks63LzaO6IA0K1vfM4qO6IFtIaXN0b25ldF0gSUhD IG9uIGZyb3plbnMgDQogDQpDYW4geW91IGRvIGhlYXQgcmV0cmlldmFsIG9uIGZyb3plbnM/DQpL aW1iZXJseSBDLiBUdXR0bGUgIEhUIChBU0NQKQ0KUGF0aG9sb2d5IEJpb3JlcG9zaXRvcnkgYW5k IFJlc2VhcmNoIENvcmUNClVuaXZlcnNpdHkgb2YgTWFyeWxhbmQgDQpSb29tIE5CVzU4LCBVTU1D DQoyMiBTLiBHcmVlbmUgU3QNCkJhbHRpbW9yZSwgTUQgMjEyMDENCig0MTApIDMyOC01NTI0DQoo NDEwKSAzMjgtNTUwOCBmYXggDQpQbGVhc2UgY29uc2lkZXIgdGhlIGVudmlyb25tZW50IGJlZm9y ZSBwcmludGluZyB0aGlzIGUtbWFpbC4NCg0KVGhpcyBlLW1haWwgYW5kIGFueSBhY2NvbXBhbnlp bmcgYXR0YWNobWVudHMgbWF5IGJlIHByaXZpbGVnZWQsIGNvbmZpZGVudGlhbCwgY29udGFpbiBw cm90ZWN0ZWQgaGVhbHRoIGluZm9ybWF0aW9uIGFib3V0IGFuIGlkZW50aWZpZWQgcGF0aWVudCBv ciBiZSBvdGhlcndpc2UgcHJvdGVjdGVkIGZyb20gZGlzY2xvc3VyZS4gU3RhdGUgYW5kIGZlZGVy YWwgbGF3IHByb3RlY3QgdGhlIGNvbmZpZGVudGlhbGl0eSBvZiB0aGlzIGluZm9ybWF0aW9uLiBJ ZiB0aGUgcmVhZGVyIG9mIHRoaXMgbWVzc2FnZSBpcyBub3QgdGhlIGludGVuZGVkIHJlY2lwaWVu dDsgeW91IGFyZSBwcm9oaWJpdGVkIGZyb20gdXNpbmcsIGRpc2Nsb3NpbmcsIHJlcHJvZHVjaW5n IG9yIGRpc3RyaWJ1dGluZyB0aGlzIGluZm9ybWF0aW9uOyB5b3Ugc2hvdWxkIGltbWVkaWF0ZWx5 IG5vdGlmeSB0aGUgc2VuZGVyIGJ5IHRlbGVwaG9uZSBvciBlLW1haWwgYW5kIGRlbGV0ZSB0aGlz IGUtbWFpbC4NCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f DQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVk dQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3Rv bmV0DQo= From mucram11 <@t> comcast.net Thu Jul 2 12:29:28 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Jul 2 12:29:31 2009 Subject: [Histonet] Need Help Message-ID: <426867284.37611246555768622.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I need to reach Charlie Dorner.? If anyone can get her this message I would appreciate it.? Thanks, Pam Marcum From rjbuesa <@t> yahoo.com Thu Jul 2 13:19:30 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 2 13:19:33 2009 Subject: [Histonet] IHC on frozens Message-ID: <728241.86517.qm@web65705.mail.ac4.yahoo.com> Kimberly: Your question has a two parts answer: 1- it cannot be done because the sections will peel off, but most importantly 2- it is not necessary since HIER was developed to "undo" the cross linkage produced by the NBF fixation, and the tissues used for?FS are not fixed. ren? J.? --- On Thu, 7/2/09, Kimberly Tuttle wrote: From: Kimberly Tuttle Subject: [Histonet] IHC on frozens To: "histonet" Date: Thursday, July 2, 2009, 12:55 PM Can you do heat retrieval on frozens? Kimberly C. Tuttle? HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srnty <@t> aol.com Thu Jul 2 13:42:35 2009 From: srnty <@t> aol.com (srnty@aol.com) Date: Thu Jul 2 13:43:33 2009 Subject: [Histonet] Help With Hemo Fading Message-ID: <8CBC95C000FAC8E-F10-409@WEBMAIL-DY11.sysops.aol.com> Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg From rjbuesa <@t> yahoo.com Thu Jul 2 14:27:55 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 2 14:27:59 2009 Subject: [Histonet] Help With Hemo Fading Message-ID: <484983.51369.qm@web65711.mail.ac4.yahoo.com> The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable "culprit" for the henatoxylin fading. Check the staining protocol. Ren? J. --- On Thu, 7/2/09, srnty@aol.com wrote: From: srnty@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Jul 2 14:32:50 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jul 2 14:32:54 2009 Subject: [Histonet] Reagent Labeling Message-ID: <57BE698966D5C54EAE8612E8941D76830608873C@EXCHANGE3.huntingtonhospital.com> Is it required by CAP to label all alcohol, xylene, etc bottles with received and opened dates? From rjbuesa <@t> yahoo.com Thu Jul 2 14:42:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 2 14:42:19 2009 Subject: [Histonet] Reagent Labeling Message-ID: <461906.29830.qm@web65704.mail.ac4.yahoo.com> As far as I know, yes! Ren? J. --- On Thu, 7/2/09, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Reagent Labeling To: "Histonet" Date: Thursday, July 2, 2009, 3:32 PM Is it required by CAP to label all alcohol, xylene, etc bottles with received and opened dates? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Thu Jul 2 16:01:29 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Jul 2 16:01:41 2009 Subject: [Histonet] Laura Miller is out of the office. Message-ID: I will be out of the office starting 07/02/2009 and will not return until 07/06/2009. I will respond to your email on Monday. Thanks! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From adamcpeer <@t> gmail.com Thu Jul 2 17:06:06 2009 From: adamcpeer <@t> gmail.com (Adam) Date: Thu Jul 2 17:06:29 2009 Subject: [Histonet] 4F:1G Message-ID: I'm new to the field of histology and want to use 4F:1G to fix fish ovaries for later histological examination under light microscopy. Following fixation, I plan to rinse the ovary samples in an ethanol series (50-95%). First, what is the minimum time needed to fix 10 g of tissue? Second, I would like to store the fixed samples for a few months prior to embedding. Is it best to maintain them in 4F:1G or to keep them in ethanol after the rinses? From stephen.asquith <@t> petermac.org Thu Jul 2 18:04:37 2009 From: stephen.asquith <@t> petermac.org (Asquith Stephen) Date: Thu Jul 2 18:05:24 2009 Subject: [Histonet] Re: Leica Autostainer Problems Message-ID: <6D3E01C097DB5C479B25F4063F6385CB0379A611@PMC-EMAIL.petermac.org.au> Hi All Many thanks for the posts that were sent regarding our problem. The local Leica agents in John and Marina came out and checked out the machine. The next day Marina spent the day with us trouble shooting with a perfect H&E as an end result. The problem was sloved by including a wash step prior to the dehydration steps after the Eosin. This ensured all the eosin was removed from the slide prior to the dehydration steps. Droplets of eosin were being left on the lable portion of the slides and these then dripped down the slide removing some of the eosin stain and hence our problem. Happy histologists all round. Many thanks to Marina Stephen Asquith Microscopy Imaging and Research Core Facility Peter MacCallum Cancer Centre Locked Bag #1 A'Beckett Street East Melbourne 8006 Email: stephen.asquith@petermac.org Phone +61-3-9656 1244/1243 Fax +61-3-9656 1411 This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. From FMonson <@t> wcupa.edu Thu Jul 2 22:04:51 2009 From: FMonson <@t> wcupa.edu (Monson, Frederick) Date: Thu Jul 2 22:08:26 2009 Subject: [Histonet] Help With Hemo Fading References: <484983.51369.qm@web65711.mail.ac4.yahoo.com> Message-ID: <641CEFFC7E5B6C42AB59539653FD082301570BD4@wcu-ex-emp2.PASSHE.LCL> Hi All, Mordanted hematoxylin is quite colorfast when carefully 'blued' after binding to acid moieties in the tissues. If one analyzes the entire process of H&E dyeing, one can 'see' several steps in which the 'bluing' process can be 'undone' or 'temporized'. Note that I have always used personally prepared Gum Damar in Xylene to mount my sections. It has always behaved better than expected. Please recall that all of the currently used histologic procedures originated using water glasses from the dining room or kitchen and chemicals that were generated by magic. When I was learning the procedure using the now-antiquated Coplin jar, I was overwhelmed by the inability of distilled water to 'blue' hematoxylin after acid differentiation. For we new histologists at the time, the only thing that really 'set' the blue color was running tap water for 5 minutes AFTER 5 minutes in 35% EtOH, 2% in NaHCO3. NB, the same magic rinse was required to stabilize the magenta of the PAS stain. In those days, there was the complaint that even some tap waters in other parts of the country left some lack of color fastness with mordanted hematoxylin (it must be remembered that the active component of the H&E stain is NOT hematoxylin (those who are surprised must go the library!!) In any case, I had a colleague who noticed that the slides in his collection were fading slowly in the closet in which he stored them (stored pretty much as we all do). It was suggested that he perform a test of the storage environment in the following way. Using an aquarium aerator placed in the closet to bubble air thru 1.5 liters of distilled water with an appropriate indicator in a two-liter Ehrlenmyer with a two-hole stopper, the outlet taking the bubbled air out of the closet via an appropriate length of tubing. Modify the above aerator and water volume to insure that the water will not be significantly evaporated during the overnight process (temperature, volume, and pressure are the appropriate variables). This test should be run for several to many nights and days (or weeks) to determine whether at any time the environment 'goes' acidic. [[The upside is that the experiment/test is neither labor intensive nor expensive (no grant proposal is required), though if you can wangle some stimulus money, you must let me know, because they will want to be able to count me as one of those who was put to work - albeit briefly. If you run the above test for several years, you may be able to count me as being employed every time you refer to this suggestion. [Perhaps not ethical, but we must do anything to keep unemployment under 10% lest there be declared another economic crisis whose fix we know by now will be the expected printing of more money to fund more studies of more fish in a pond or bugs on a wall.]] Back to point, if at any time one observed the water to go acid, then the location of the storage is a likely candidate. If you use a machine to stain your sections, as many to most do, then perhaps another environmental characteristic (such as humidity) is causing either continuous or seasonal ethanol dilution and thus leaving too much water in the sections, even though they do not appear milky when mounted. In such a case, one must expect faster diffusion of acidic materials into the preparation. NB, I only consider those airborne substances that are hydrogen rich - they can be inorganic or organic - simply anything that gets sprayed, mixed, dumped in the sink - anything that continuously gets aerosoled in the lab or is blown in with the 'conditioned' air. Another test you might perform involves sealing slide boxes in plastic bags - see your local molecular biologist or check the kitchen area at KMart, Penny's, or Walmart for systems that pull a vacuum around food and seal it by creating a heat seal. [If still unfamiliar, call Mom OR Mom-in-law!]. You must have a control box to compare over time. One assumes that NOTHING can get in or out of such a sealed container. The ultimate downside is that one does not or cannot control a process that one trusts to be automatic and credible. Hematologists may take note: At the age of 40 I was laid up with a pulmonary 'problem' that included a few days with 104-105 degree spikes, dry coughing, and visits from the Savior who I sent away screaming, "Hell No! I won't go!" - and in the process scarring the b'Jesus out of my kids. In any case after passing the hospitalization test and ahaving received, i.v., every known broad spectrum antibiotic in the pharmacy, I got my doctors to give me erythromysin i.v.("we don't give enythromysin orally or i.v. - it bites!), the temp went down, and I felt better. [If legionella is sensitive to erythromysin, why did so many die from that bug in Philadelphia? (This will not be on the test, but it should be.)] Thus, came the day when I was told that my blood differential was so messy that it had been recommended that I submit to a bone marrow exam to determine my proximity to death. I quietly demanded to know how the differential was done. The answer? The Coulter counter! I quietly agreed to a bone marrow exam AFTER a manual differential was performed. Later, the hematologist only wanted to know how I knew. I told her that while working in a path lab at night during college (I know I wasn't 'certified'!!!), I had compared myself to the 'Counter' and I was frankly more consistent. I admitted that my test was not statistical, but I had real confidence in the capacity of the eye and little in the photometer looking thru glass. Machines may be necessary to do the work, but they do not clean themselves and they do not cry out when the 95% ethanol has become 87%. With Coplin jars, variables were easy to manage. They were more difficult the more we tried to do multiples of specimens. This should be obvious in spite of the fact that automation is practical and manual is not - even though sometimes necessary. When I started in histology, I began at the beginning of the Paraplast revolution, yet there were still those who formulated their own paraffin for the part of the planet in which they worked. Sorry for the late-night babble, but I have vented stored up compulsion. Cheers, Fred Monson P.S. Those who are required to volunteer do NOT. Those who require others to volunteer fail. Every tax is a means by which a government forces a citizen to pay for the vacations of those who work for the government. When the USA has a public health plan, every union will be forced to accept it by the employer, and all those who want something better will be required to pay for the full load. Government plan - 'free', all others - cost extra. That's competition? Why won't the insurance companies want to give 'free' public health care to their employees? Their own actuaries will show them the way. Three of four wars prosecuted by the United States during the 20th century were begun under democratically elected Presidents. One was fought entirely by volunteers. During only two of the four have a majority of citizens, at one time or another, wished the U.S.A. to lose(???). Three cheers for the Draft!!!!! Beware the resolve of an elected President not to lose. Frederick C. Monson, PhD Technical Director, CMIRT West Chester University West Chester, PA, 19383 610-738-0437 http://cmirt.wcupa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 7/2/2009 3:27 PM To: histonet@lists.utsouthwestern.edu; srnty@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable "culprit" for the henatoxylin fading. Check the staining protocol. Ren? J. --- On Thu, 7/2/09, srnty@aol.com wrote: From: srnty@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jul 3 02:09:10 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 3 02:09:15 2009 Subject: [Histonet] Help With Hemo Fading Message-ID: <86ADE4EB583CE64799A9924684A0FBBF07012E0A@wahtntex2.waht.swest.nhs.uk> I agree...... Also you're not storing them in sunlight are you? Silly question I know. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 02 July 2009 20:28 To: histonet@lists.utsouthwestern.edu; srnty@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable "culprit" for the henatoxylin fading. Check the staining protocol. Ren? J. --- On Thu, 7/2/09, srnty@aol.com wrote: From: srnty@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Jul 3 04:59:22 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Jul 3 04:59:28 2009 Subject: [Histonet] Help With Hemo Fading In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF07012E0A@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF07012E0A@wahtntex2.waht.swest.nhs.uk> Message-ID: Even long term exposure to indoor lighting (fluorescent type) will fade sectiions. We work in a building where ther lights are permanently on. If we want to keep our sections fresh and helathy, they get covered up as soon as . best regards On Fri, Jul 3, 2009 at 9:09 AM, Kemlo Rogerson < Kemlo.Rogerson@waht.swest.nhs.uk> wrote: > I agree...... Also you're not storing them in sunlight are you? Silly > question I know. > > > > Kemlo Rogerson > e-mail kemlorogerson@nhs.net if not at work. > DD 01934 647057 or extension 3311 Mob 07749 754194; > Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah > Lehrer > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: 02 July 2009 20:28 > To: histonet@lists.utsouthwestern.edu; srnty@aol.com > Subject: Re: [Histonet] Help With Hemo Fading > > The fading most probably is caused by acid in the permanent slide, probably > because the sections were passed through the alcohols very quickly after the > acid differentiation, or they stayed little time in tap water after > differentiation or no bluing agent was used. > It is unlikely that the mounting medium is acidic, although that could also > be the cause also. An acid environment over the cover slipped section is the > most probable "culprit" for the henatoxylin fading. > Check the staining protocol. > Ren? J. > > --- On Thu, 7/2/09, srnty@aol.com wrote: > > > From: srnty@aol.com > Subject: [Histonet] Help With Hemo Fading > To: histonet@lists.utsouthwestern.edu > Date: Thursday, July 2, 2009, 2:42 PM > > > > Hello everyone, > > ? > > We are having problems with short-term hematoxylin fading and loss of > detail. The pathologist is freaking out! I've seen hemo fade over a long > period of time but not in a matter of a few months. Slides from one year ago > are really bad. > > ? > > I've been out of the business for a number of years and in the interim much > has changed including reagents. These are GI tract biopsies processed by > microwave. > > ? > > Any thoughts at all? > > ? > > Thanks! Marg > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From milton.gomez <@t> aruplab.com Fri Jul 3 05:28:21 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Fri Jul 3 05:28:41 2009 Subject: [Histonet] New Ventana IHC stainer Message-ID: Dear Histonetters: Do you folks recommend getting a maintenance contract on a new ventana instrument? May you share your protocol for setting up the instrument? Thanks in advance, MG - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From milton.gomez <@t> aruplab.com Fri Jul 3 11:09:56 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Fri Jul 3 11:10:16 2009 Subject: [Histonet] IHC stainer Message-ID: Dear Histonetters: What is the best, most reliable workhorse immunostainer out there nowadays? and why. Thanks in advance, MG - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From peddinti_2002us <@t> yahoo.co.in Fri Jul 3 22:01:14 2009 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Fri Jul 3 22:01:22 2009 Subject: [Histonet] Fading of H E sections Message-ID: <587127.92343.qm@web95206.mail.in2.yahoo.com> Mostly it could be the acidic mounting media. Please check the pH of mounting media. ? Kamal See the Web's breaking stories, chosen by people like you. Check out Yahoo! Buzz. http://in.buzz.yahoo.com/ From kmerriam2003 <@t> yahoo.com Mon Jul 6 06:31:57 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Jul 6 06:32:02 2009 Subject: [Histonet] Fw: [champ] website for introducing Molecular diagnostics, proteomics etc Message-ID: <911380.13749.qm@web50311.mail.re2.yahoo.com> Hi All, I subscribe to another listserve and I thought this link might be of interest to people on the histonet (see the link below): ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ----- Forwarded Message ---- From: Deborah Payne To: AMP Membership Listserv Cc: aacc-proteomics-div@aacclists.org Sent: Friday, June 26, 2009 3:59:58 PM Subject: [champ] website for introducing Molecular diagnostics, proteomics etc Dear Colleagues, ? The link below is from the NCI and it has several ppt on numerous topics of interest. ? http://www.cancer.gov/cancertopics/understandingcancer/moleculardiagnostics ? ? I thought I would share this. ?The ppt are a bit simple but still very good. ? debs * ------------------You are currently subscribed to the CHAMP Listserv. This listserv is exclusively for the membership of the Association for Molecular Pathology. While discussion of commercial technologies and products frequently appears on CHAMP, the listserv is not intended as a medium for distribution of commercial advertisements. We encourage our members to keep this in mind when making postings. AMP will not publish and/or post material that is primarily commercial in nature. Having trouble posting CHAMP messages? Maybe your e-mail address has changed and we need to change your CHAMP listing. Please send us e-mail at: AMP@asip.org To unsubscribe, please email Ann Marie Bocus, Membership Manager, at ambocus@amp.org American Society for Investigative Pathology 9650 Rockville Pike Bethesda, MD 20814 From litepath2000 <@t> yahoo.com Mon Jul 6 10:38:34 2009 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Mon Jul 6 10:38:38 2009 Subject: [Histonet] Fw: Histology supervisor Message-ID: <780724.69812.qm@web58806.mail.re1.yahoo.com> Job Description: *Title:* Histology Supervisor *Department:* Pathology *Location:* Park Slope *Shift:* Full Time, Days *Description:* In this role the Histology Supervisor is responsible for performing a variety of administrative and supervisory functions relating to Histology and other sections as directed by the Administrative Director. This candidate will be responsible for preparing for CAP and NYSDOH inspections and participates in proficiency testing. Responsible for adhering to the rules established by the regulatory agencies governing the practice of Histology. Has the authority to select, discipline and discharge personnel. Oversees Cytology in the supervisors absence and monitors morgue issues. Will perform all duties assigned consistent with New York Methodist Hospitals commitment to providing quality health care services in a compassionate and humane manner. *Requirements:* B.S. in Health Science is required. NYSSED license or elgible. A.S.C.P.(HT/HTL)certification preferred. Seeking candidate with five years experience in Histology laboratory. Supervisory experienced highly preferred. Strong communication and organization skills required. Siobhan O'Reilly Administrative Director, Pathology and Laboratory Medicine New York Methodist Hospital Brooklyn, NY p: 718-780-3653 f: 718-780-3673 www.nym.org -------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited.? If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message.? Thank you. From TJJ <@t> stowers.org Mon Jul 6 12:09:05 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Jul 6 12:10:09 2009 Subject: [Histonet] Re: IHC on frozens Message-ID: In response to this thread: >< Kimberly: Your question has a two parts answer: 1- it cannot be done because the sections will peel off, but most importantly 2- it is not necessary since HIER was developed to "undo" the cross linkage produced by the NBF fixation, and the tissues used for?FS are not fixed. ren? J.? --- On Thu, 7/2/09, Kimberly Tuttle wrote: From: Kimberly Tuttle Subject: [Histonet] IHC on frozens To: "histonet" Date: Thursday, July 2, 2009, 12:55 PM Can you do heat retrieval on frozens? Kimberly C. Tuttle? HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >< I disagree that you cannot do HIER on frozen sections. We do them all the time. All our samples are fixed in formalin, and then cryoprotected in sucrose prior to freezing them, so providing an antigen retrieval step usually produces good IHC results in frozen sections. We section and dry the slides, then use citrate buffer pH 6.0 in the microwave at 60 degrees C for 10 minutes, cool 10 minutes, then rinse and continue with IHC protocol. Use Plus slides and keep the solution under boiling temperature and you should be fine. For things like brain or bone, you might want to use lower temperatures for longer period of time instead of high temps for less time. It can even work in fresh-frozen samples which are sectioned and then fixed prior to immunostaining. Some great information on this is included in this article: Yamashita and Okada, Application of Heat-induced Antigen Retrieval to Aldehyde-fixed Fresh Frozen Sections, JHC Vol 53(11): 1421-1432, 2005. A big thanks to Gayle Callis for the heads up on this paper! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From jsjurczak <@t> att.net Mon Jul 6 12:31:16 2009 From: jsjurczak <@t> att.net (jeff jurczak) Date: Mon Jul 6 12:31:19 2009 Subject: [Histonet] Looking for Jim Robinson Message-ID: <554141.50021.qm@web82204.mail.mud.yahoo.com> Jim, Could you contact me? Jeff Jurczak From NSEARCY <@t> swmail.sw.org Mon Jul 6 12:37:39 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Mon Jul 6 12:37:47 2009 Subject: [Histonet] ER/PR HER2 Redux Message-ID: <4A51F013.5D38.00EF.0@swmail.sw.org> Funny, I thought I had this issue taken care of but we are once again discussing it! What are others doing to verify formalin fixation times? Is it "good enough" to have a blanket statement in the report that we are in compliance of the 6-48 hour required time ( we have fixation time on container dictated into report & we process on the weekend ) or is the actual hours of fixation ( time in formalin container+ time in processor = total fixation time) documented into the report? And do you document ALL? So that even those that are unsuspected (reductions) get a fixation ( just in case). Discussion on Wednesday. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From Robert.Cordero <@t> comphealth.com Mon Jul 6 12:57:56 2009 From: Robert.Cordero <@t> comphealth.com (Robert.Cordero@comphealth.com) Date: Mon Jul 6 12:58:10 2009 Subject: [Histonet] - Referral needed for day shift Histology opening in Southern New Mexico In-Reply-To: <4A51F013.5D38.00EF.0@swmail.sw.org> Message-ID: Hello! I was wondering if anyone knew of a Histo tech who would be interested in a day shift, staff position in southern New Mexico. The facility will look at a strong new graduate. They are offering relocation assistance and possibly temporary housing. They have a few available apartments but it is first come, first serve. The tentative start date will be early August, but the facility is wanting to interview and hire here in the next few weeks. If you know of someone who might be interested, please forward my information along or forward me their information. We are offering a referral bonus for this opportunity. Thank you in advance! Regards, Rob Cordero Laboratory Sciences Consultant Permanent Placement Division CompHealth Associates, Inc. Phone: 866-782-9029 x 5866 Direct: 954-343-5866 Fax: 800-420-2329 E-mail: robert.cordero@comphealth.com www.comphealth.com About us: http://www.comphealth.com/about_us/about Customer service is the key to success. Are you satisfied with your experience? Send your comments to my manager at valerie.patterson@comphealth.com. Please note: "This is a commercial email from CompHealth Associates, Inc. If you do not want to receive future emails from CompHealth Associates, Inc., please reply to the sender of this email and ask to be removed from our list." From cforster <@t> umn.edu Mon Jul 6 13:34:26 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jul 6 13:34:29 2009 Subject: [Histonet] Re: IHC on frozens In-Reply-To: References: Message-ID: <4A5243B2.6010807@umn.edu> I disagree. I do heat retrieval on frozen sections that have been fixed in a formalin fix. If you use the correct protocol you can get very nice IHC on these. Colleen Forster HT(ASCP)QIHC Anatomic Pathology Research Laboratory U of MN Johnson, Teri wrote: > In response to this thread: > > >> < >> > Kimberly: > Your question has a two parts answer: > 1- it cannot be done because the sections will peel off, but most importantly > 2- it is not necessary since HIER was developed to "undo" the cross linkage produced by the NBF fixation, and the tissues used for?FS are not fixed. ren? J.? > > --- On Thu, 7/2/09, Kimberly Tuttle wrote: > > > From: Kimberly Tuttle > Subject: [Histonet] IHC on frozens > To: "histonet" > Date: Thursday, July 2, 2009, 12:55 PM > > > Can you do heat retrieval on frozens? > > Kimberly C. Tuttle? HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > Please consider the environment before printing this e-mail. > >> < >> > > I disagree that you cannot do HIER on frozen sections. We do them all the time. All our samples are fixed in formalin, and then cryoprotected in sucrose prior to freezing them, so providing an antigen retrieval step usually produces good IHC results in frozen sections. We section and dry the slides, then use citrate buffer pH 6.0 in the microwave at 60 degrees C for 10 minutes, cool 10 minutes, then rinse and continue with IHC protocol. Use Plus slides and keep the solution under boiling temperature and you should be fine. For things like brain or bone, you might want to use lower temperatures for longer period of time instead of high temps for less time. > > It can even work in fresh-frozen samples which are sectioned and then fixed prior to immunostaining. Some great information on this is included in this article: Yamashita and Okada, Application of Heat-induced Antigen Retrieval to Aldehyde-fixed Fresh Frozen Sections, JHC Vol 53(11): 1421-1432, 2005. A big thanks to Gayle Callis for the heads up on this paper! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tfountain <@t> exchange.hsc.mb.ca Mon Jul 6 13:40:56 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Mon Jul 6 13:41:01 2009 Subject: [Histonet] Digital Slide Imaging Message-ID: Hi Everyone, What are the current thoughts on digital slide imaging systems like Aperio, etc. I am interested in other people's experience with slide imaging systems and their preferences. Any opinions/suggestions would be appreciated! Tiana Baskin -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From ploykasek <@t> phenopath.com Mon Jul 6 14:28:55 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Jul 6 14:29:10 2009 Subject: [Histonet] IHC frozens Message-ID: Hi Kim. You can do HIER on frozen sections, but first you must fix the sections. The purpose of HIER is to 'unmask' antigens that have been altered by the effects of fixation. We have had better results with many antibodies by fixing the frozen sections in 10% buffered formalin (30'-60'), then doing a gentle heat retrieval (~70C) or using a mild enzyme. Of course, there are times when it is preferable to use the frozen sections as is & determine if the antibody will recognize the epitope in this 'native' state. Just my experience. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From MDiCarlo <@t> KaleidaHealth.Org Mon Jul 6 14:33:34 2009 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Mon Jul 6 14:33:43 2009 Subject: [Histonet] Accu-edge sales rep Message-ID: <1B73766A27A1554CB2729B6432E81301015C7241@KALEXMB04.KaleidaHealth.org> Hello, I just opened a brand new box of Accu-Edge high profile blades and I cannot get the very first blade to slide out of the container. Even though I have had the box of unopened blades for some time, I was wondering if you can guide me in some way that I can retrieve these blades without cutting myself or be able to compensate me with a new box and I would gladly return the box of sticky blades. I would greatly appreciate your help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From mpence <@t> grhs.net Mon Jul 6 14:44:28 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jul 6 14:44:34 2009 Subject: [Histonet] ER/PR HER2 Redux In-Reply-To: <4A51F013.5D38.00EF.0@swmail.sw.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BA7@is-e2k3.grhs.net> If I were inspecting you, I would ask you to show me how you are in compliance. I would not stop until you showed me documented times in formalin. I would want to see something like " Formalin fixation time in 10% NBF is 18 hours" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Monday, July 06, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR HER2 Redux Funny, I thought I had this issue taken care of but we are once again discussing it! What are others doing to verify formalin fixation times? Is it "good enough" to have a blanket statement in the report that we are in compliance of the 6-48 hour required time ( we have fixation time on container dictated into report & we process on the weekend ) or is the actual hours of fixation ( time in formalin container+ time in processor = total fixation time) documented into the report? And do you document ALL? So that even those that are unsuspected (reductions) get a fixation ( just in case). Discussion on Wednesday. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 From tkngflght <@t> yahoo.com Mon Jul 6 15:03:21 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Jul 6 15:02:25 2009 Subject: [Histonet] Jobs - both temp and permanent Message-ID: <268B42DE62634C27AA34F540714F7A35@FULLSTAFF.ORG> Hi everyone- I haven't posted in a long time--though I've been reading every day! In light of the changes in the economy and our work situations, we're getting more and more permanent & temp-to-permanent openings than temporary travel opportunities. We still have travel jobs and are always looking for all shifts, Mohs, and other specialties, and we're getting more permanent conversations than ever before. Are you seeking a place to settle down? A good job where you look FORWARD to going to work? A community that fits and helps you grow instead of a job you merely tolerate because you need the paycheck... Rather than list a bunch of available jobs and shoe-horning you into one, let us help you find your 'lobster' job. The one that makes a difference for you and in turn--you make a difference for your Pathologists and the patients they serve. Our permanent openings are ALL over the place from routine bench through Manager/Coordinator. As a working histotech, I catch all the incoming Histology calls--I want to hear what moves you, makes you happy, gets you fired up as a histotech. We'll even help you write your resume in a way that shows off what sets you apart. We're not all round pegs, but we all deep down want to know we make a difference. Shoot us what you have for a resume (even that old messy one), email an informal paragraph on what you dream about, or just call. You'll talk with me--a working histotech. I take all first-round histology inquiries. The conversation is simple--I get it. You won't have to explain what you do--I've been a working tech since 1983. Please leave a message if it goes to voicemail. I will call you back. Cheryl Cheryl R. Kerry, Principal, HT(ASCP) Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time! 281.852.9457 800.756.3309 eFax From linhines <@t> yahoo.com Mon Jul 6 15:17:23 2009 From: linhines <@t> yahoo.com (Linda Hines) Date: Mon Jul 6 15:17:26 2009 Subject: [Histonet] Re:romove me from your lisr Message-ID: <479525.52952.qm@web55402.mail.re4.yahoo.com> Please remove me from the histonet. Thank you linda hines From carrolpb <@t> umdnj.edu Mon Jul 6 15:22:59 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Jul 6 15:23:09 2009 Subject: [Histonet] Re:romove me from your lisr In-Reply-To: <479525.52952.qm@web55402.mail.re4.yahoo.com> References: <479525.52952.qm@web55402.mail.re4.yahoo.com> Message-ID: <4A525D23.5070700@umdnj.edu> > Please remove me from the histonet. Thank you linda hines In case you missed it the first 500 times... :-0 ______________________________________________________ LEAVING Send an email to listserv@lists.umn.edu (and NOT confocalmicroscopy@lists.umn.edu) and put the following into the body of the message: *signoff confocalmicroscopy* (note that it *isn't* "signoff confocal") Alternatively, a subscriber can go to the website http://lists.umn.edu/ and follow the directions on the web page. From Sandy.Schmitz <@t> leica-microsystems.com Mon Jul 6 16:01:29 2009 From: Sandy.Schmitz <@t> leica-microsystems.com (Sandy.Schmitz@leica-microsystems.com) Date: Mon Jul 6 16:01:37 2009 Subject: [Histonet] Schmitz, Sandy is out of the office. Message-ID: I will be out of the office starting 07/02/2009 and will not return until 07/07/2009. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From laurie.colbert <@t> huntingtonhospital.com Mon Jul 6 16:24:44 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jul 6 16:24:47 2009 Subject: [Histonet] ER/PR HER2 Redux Message-ID: <57BE698966D5C54EAE8612E8941D768306088900@EXCHANGE3.huntingtonhospital.com> We document total fixation time, including the time in formalin on the processor, in the report. We do not document times on breast reductions. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Monday, July 06, 2009 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR HER2 Redux Funny, I thought I had this issue taken care of but we are once again discussing it! What are others doing to verify formalin fixation times? Is it "good enough" to have a blanket statement in the report that we are in compliance of the 6-48 hour required time ( we have fixation time on container dictated into report & we process on the weekend ) or is the actual hours of fixation ( time in formalin container+ time in processor = total fixation time) documented into the report? And do you document ALL? So that even those that are unsuspected (reductions) get a fixation ( just in case). Discussion on Wednesday. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 From mshudgins <@t> bellsouth.net Mon Jul 6 18:22:13 2009 From: mshudgins <@t> bellsouth.net (MATT & SIRENA HUDGINS) Date: Mon Jul 6 18:22:16 2009 Subject: [Histonet] (no subject) Message-ID: <233615.28614.qm@web180405.mail.gq1.yahoo.com> HI, ? Can anyone tell me if you've ever seen a jaw type chuck holder (or adapter) for a cm1850 leica cryostat. I am getting in some frozen tissue that has been embedded in square embedding rings. I have an adapter for for square blocks, but the clamp only consist of a screw holding the block in place. It simply is not?stable enough to hold my embedding ring. Any suggestions? ????????????????????????????????????????????? Thanks, ?????????????????????????????????????????????? Sirena HT (ascp) From tifei <@t> foxmail.com Tue Jul 7 06:17:36 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Tue Jul 7 06:17:57 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIFJlOiBJSEMgb24gZnJvemVucw==?= References: , <4A5243B2.6010807@umn.edu> Message-ID: <200907071917310753998@foxmail.com> it is because when mentioning "frozen section", we r talking about different procedures. some fix brain, sucrose sink, then perform OCT embedding and cryostat section;you can use enzyme digestion or HIER for antigen retrieval some other use fresh brain, snap freezing, and cryostat section; this approach does not require HIER. 2009-07-07 TF ???? Colleen Forster ????? 2009-07-07 02:41:59 ???? Johnson, Teri ??? histonet@lists.utsouthwestern.edu ??? Re: [Histonet] Re: IHC on frozens I disagree. I do heat retrieval on frozen sections that have been fixed in a formalin fix. If you use the correct protocol you can get very nice IHC on these. Colleen Forster HT(ASCP)QIHC Anatomic Pathology Research Laboratory U of MN Johnson, Teri wrote: > In response to this thread: > > >> < >> > Kimberly: > Your question has a two parts answer: > 1- it cannot be done because the sections will peel off, but most importantly > 2- it is not necessary since HIER was developed to "undo" the cross linkage produced by the NBF fixation, and the tissues used for?FS are not fixed. ren? J.? > > --- On Thu, 7/2/09, Kimberly Tuttle wrote: > > > From: Kimberly Tuttle > Subject: [Histonet] IHC on frozens > To: "histonet" > Date: Thursday, July 2, 2009, 12:55 PM > > > Can you do heat retrieval on frozens? > > Kimberly C. Tuttle? HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > Please consider the environment before printing this e-mail. > >> < >> > > I disagree that you cannot do HIER on frozen sections. We do them all the time. All our samples are fixed in formalin, and then cryoprotected in sucrose prior to freezing them, so providing an antigen retrieval step usually produces good IHC results in frozen sections. We section and dry the slides, then use citrate buffer pH 6.0 in the microwave at 60 degrees C for 10 minutes, cool 10 minutes, then rinse and continue with IHC protocol. Use Plus slides and keep the solution under boiling temperature and you should be fine. For things like brain or bone, you might want to use lower temperatures for longer period of time instead of high temps for less time. > > It can even work in fresh-frozen samples which are sectioned and then fixed prior to immunostaining. Some great information on this is included in this article: Yamashita and Okada, Application of Heat-induced Antigen Retrieval to Aldehyde-fixed Fresh Frozen Sections, JHC Vol 53(11): 1421-1432, 2005. A big thanks to Gayle Callis for the heads up on this paper! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Jul 7 09:24:54 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Jul 7 09:25:01 2009 Subject: [Histonet] Tween 20 Message-ID: <74C6DA725C716D8A22324961@CDYwxp1931.ad.med.buffalo.edu> Hi all, What is the difference between Enzyme Grade Tween 20 and Molecular Biology Grade Tween 20? Thanks! Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Janet.Bonner <@t> FLHOSP.ORG Tue Jul 7 09:28:27 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jul 7 09:31:53 2009 Subject: [Histonet] Accu-edge sales rep References: <1B73766A27A1554CB2729B6432E81301015C7241@KALEXMB04.KaleidaHealth.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2A96@fhosxchmb006.ADVENTISTCORP.NET> I've had the same problem. I started keeping them in a ziplock with desiccant packets and it seems to help. Even then I have to bang them top-down to advance the blades to the top so they catch on to the slider mechanism. I don't know why I put up with this - except that I'm awfully busy doing Histology!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Mon 7/6/2009 3:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Accu-edge sales rep Hello, I just opened a brand new box of Accu-Edge high profile blades and I cannot get the very first blade to slide out of the container. Even though I have had the box of unopened blades for some time, I was wondering if you can guide me in some way that I can retrieve these blades without cutting myself or be able to compensate me with a new box and I would gladly return the box of sticky blades. I would greatly appreciate your help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From mcauliff <@t> umdnj.edu Tue Jul 7 09:36:02 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jul 7 09:34:31 2009 Subject: [Histonet] Tween 20 In-Reply-To: <74C6DA725C716D8A22324961@CDYwxp1931.ad.med.buffalo.edu> References: <74C6DA725C716D8A22324961@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <4A535D52.7080301@umdnj.edu> Ask the manufacturer, they will know. Or look at the MSDS Geoff Merced M Leiker wrote: > Hi all, > > What is the difference between Enzyme Grade Tween 20 and Molecular > Biology Grade Tween 20? > > Thanks! > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Tue Jul 7 09:39:20 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jul 7 09:37:47 2009 Subject: [Histonet] Accu-edge sales rep In-Reply-To: <1B73766A27A1554CB2729B6432E81301015C7241@KALEXMB04.KaleidaHealth.org> References: <1B73766A27A1554CB2729B6432E81301015C7241@KALEXMB04.KaleidaHealth.org> Message-ID: <4A535E18.1020602@umdnj.edu> I had a box of low profile blades with the same problem. The vendor sent me a new box. Geoff DiCarlo, Margaret wrote: > Hello, > > > > I just opened a brand new box of Accu-Edge high profile blades and I > cannot get the very first blade to slide out of the container. Even > though I have had the box of unopened blades for some time, I was > wondering if you can guide me in some way that I can retrieve these > blades without cutting myself or be able to compensate me with a new box > and I would gladly return the box of sticky blades. > > > > I would greatly appreciate your help. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2009 Best Places to Work Winner > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Tue Jul 7 09:43:20 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jul 7 09:41:46 2009 Subject: [Histonet] Digital Slide Imaging In-Reply-To: References: Message-ID: <4A535F08.5090005@umdnj.edu> We had our set of instructional slides for Histology (medical student course) scanned at 20X and are very happy with the results. Students load the slides and viewing program on their laptops and study wherever. Higher mag scanning (40X) is necessary for finer cellular detail. I don't know if even 40X would be sufficient for hematology. Geoff Tiana Fountain wrote: > Hi Everyone, > > What are the current thoughts on digital slide imaging systems like > Aperio, etc. I am interested in other people's experience with slide > imaging systems and their preferences. > > Any opinions/suggestions would be appreciated! > > Tiana Baskin > > ------------------------------------------------------------------------ > > This email and/or any documents in this transmission is intended for the > addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. > > Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From relia1 <@t> earthlink.net Tue Jul 7 10:48:39 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jul 7 10:48:45 2009 Subject: [Histonet] RELIA Histology Management Job Alert 7/7/09 Histology Supervisor/Manager needed in San Antonio, TX Message-ID: Hi Histonetters!! I hope everyone had a safe and happy 4th of July weekend. I have a new position that I am very excited about and wanted to let you know about. This is a full time permanent position with one of my best clients. They offer excellent salary, benefits and relocation/sign on bonus. My client is a private pathology lab and they are in need of a shift production supervisor. This position offers an excellent opportunity for growth and expansion. My client is looking for someone with big lab production skills who is good with managing people and workflow. No administrative except keeping production and QS records. If you or anyone you know might be interested in this position please contact me I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Have a great day!! Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Dorothy.L.Webb <@t> HealthPartners.Com Tue Jul 7 12:45:47 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Jul 7 12:45:52 2009 Subject: [Histonet] Alk-1 controls Message-ID: <65365F35C0F2EF4D846EC3CA73E49C437AA21CB1AA@HPEMX3.HealthPartners.int> Does anyone have a good source to purchase ALK-1 IHC controls from? We were getting them from Cell Marque, but their source is no longer available!! Thanks for any help!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From arvidsonkristen <@t> yahoo.com Tue Jul 7 13:20:39 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Jul 7 13:20:42 2009 Subject: [Histonet] Error corrections Message-ID: <110074.52091.qm@web65706.mail.ac4.yahoo.com> How are people typically correcting errors that occur.? For example, mislabeled slides and path slips.? We do a lot of hand writing in our lab and when we make a mistake people are usually whiting out or scribbling.? I'm feeling like we shouldn't be doing this....is there a standard like a line through the error?with the person's initials or something? The other thing is that we do all the grossing (Derm) and we do not dictate, we draw a picture of the specimen and write down the measurements.? If the picture is wrong people white it out and re-draw it.? I hope I'm making sense.? Any suggestions? From thecitan <@t> yahoo.com Tue Jul 7 13:40:31 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Tue Jul 7 13:40:08 2009 Subject: [Histonet] Hemo-d xylene substitute in processor Message-ID: <978506345-1246992003-cardhu_decombobulator_blackberry.rim.net-12532334-@bxe1123.bisx.prod.on.blackberry> Hi everyone. I was recently contracted to run a small routine derm lab. This is the first lab i've seen a lab use hemo-d as a xylene substitute. Are there any properties of this I should keep in mind while using it in processing / staining. Sent from my Verizon Wireless BlackBerry From Rcartun <@t> harthosp.org Tue Jul 7 15:07:38 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jul 7 15:07:45 2009 Subject: [Histonet] CoPath Question Message-ID: <4A5372C9.7400.0077.1@harthosp.org> Periodically, we receive a call from an angry physician who wants to know why the specimen he or she sent to us has not been signed out. For those of you using CoPath as your Anatomic Pathology LIS, do you have a protocol (or system) for identifying cases that have not been signed out? We run reports for each pathologist to identify these cases, but, for some reason, we miss some cases. Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From syinan <@t> ucalgary.ca Tue Jul 7 15:50:31 2009 From: syinan <@t> ucalgary.ca (Salim Yalcin Inan) Date: Tue Jul 7 15:51:15 2009 Subject: [Histonet] methylene blue microinjection Message-ID: Dear All, Does anyone use intracerebroventricular methylene blue injection for the validation of drug/electrode injection/placement accuracy? Could you please give me the catalogue number of methylene blue, so I can order for myself? And any other advices would be greatly appreciated. Thank you very much in advance. Salim syinan@ucalgary.ca From pruegg <@t> ihctech.net Tue Jul 7 20:20:44 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jul 7 20:20:48 2009 Subject: [Histonet] ventana IHC and special stains users Message-ID: <0A6807B81C7F42BC8516ECBFC86A91D9@prueggihctechlt> Please for those of you using a Ventana IHC instrument and/or special stains instrument, I am interested in what you do to dispose of your toxic waste. I know each municipality is different so let me know what you do and where you live. Ventana does not separate toxic from non toxic waste so I imagine there is a lot of toxic waste to deal with and the liquid coverslip alone is something that I would think should not go down the drain?????? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From dchihc <@t> yahoo.com Wed Jul 8 08:56:15 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Jul 8 08:56:20 2009 Subject: [Histonet] VALIDATION FOR FIXATIVES Message-ID: <632025.68531.qm@web43507.mail.sp1.yahoo.com> Does anyone out there know any reference labs that do egfr, her2 fish, k ras gene mutation, etc. that have performed validation testing for tissues fixed in anything other than 10% NBF? ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From srishan <@t> mail.holyname.org Wed Jul 8 10:05:21 2009 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Wed Jul 8 10:06:44 2009 Subject: [Histonet] technical charge Message-ID: Hello, Does anyone know what is a reasonable charge (technical only ) for an unstained cut as well as per slide for cutting staining and mounting? Thanks Nirmala Srishan __________________ Can you do heat retrieval on frozens? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From ploykasek <@t> phenopath.com Wed Jul 8 10:11:02 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jul 8 10:11:12 2009 Subject: [Histonet] Job opening Message-ID: HI All. We currently have a job opening for a technologist in our molecular testing section. Here is the job posting (it is on our web site, too): Molecular Technologist (full time): PhenoPath Laboratories, PLLC, a national pathology reference laboratory, has an opportunity for a Medical Technologist or Histotechnologist in our Molecular section (full-time, M-F). Primary Responsibilities: Responsibilities include: Performing FISH and/or PCR on paraffin specimens, blood/bone marrow and other clinical specimens. Maintaining QA/QC data and writing/reviewing SOPs. Required Skills/Experience: Requirements include: ASCP certification in Medical Technology or Histotechnology required. Prefer two-plus years of molecular experience in a clinical setting; strong interpersonal skills. TO APPLY, PLEASE SUBMIT A COMPLETED APPLICATION FOR EMPLOYMENT AND RESUME TO: PhenoPath Laboratories, PLLC 551 N. 34th St., Suite 100 Seattle, WA 98103 Fax: 206 374-9009 E-mail: jobs@phenopath.com Application for employment may be downloaded from www.phenopath.com Please no phone calls regarding this position. This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From algranth <@t> email.arizona.edu Wed Jul 8 10:59:00 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Jul 8 10:59:07 2009 Subject: [Histonet] Silver Nitrate Message-ID: After a recent run on silver stains here I need to order Silver Nitrate. Can anyone tell me what the difference is (except the cost) between Silver Nitrate Certified ACS, USP, p.a., Extra Pure, and Ultra Pure? They all have the same chemical formula and FW of 169.87. My last batch of Silver Nitrate was probably ordered and received here just after the Mayflower landed and there is no information on the bottle any longer. Which one do I need for stains like GMS, Sevier Munger, etc.? Thanx, Andi 8-) Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. From jkiernan <@t> uwo.ca Wed Jul 8 11:03:43 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jul 8 11:03:49 2009 Subject: [Histonet] methylene blue microinjection Message-ID: Dear Salim Yalcin Inan, You need to explain your question! Electrodes are not ordinarily positioned to stimulate or record from the CSF in the ventricles of the brain. Methylene blue is soluble in water (see the Merck Index, Conn's Biological Stains or any other book about dyes or stains published since about 1885). How could injecting a soluble dye into one of the ventricles of the brain lead to "validation of drug/electrode injection/placement accuracy"? Methylene blue is listed in the catalogues of almost all vendors of chemicals because this dye has many uses. A Google Scholar search for "methylene blue" brings up "about 294,000 hits". You won't get the answers you need without doing a proper survey of earlier published literature (peer-reviewed papers, scholarly books etc) targeted at the questions you are trying to answer. Your email address shows that you are at a major Canadian university, but you don't declare yourself as a professor, postdoc, technician, graduate student, summer student, janitor's brush carrier or senior administrator. When asking for help you need to say who you are, as well as explain clearly what information you need. A well prepared question usually draws several answers that are helpful also to other people reading the listserver. John A. Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada = = = ----- Original Message ----- From: Salim Yalcin Inan Date: Tuesday, July 7, 2009 16:52 Subject: [Histonet] methylene blue microinjection To: Histonet > Dear All, > > > > Does anyone use intracerebroventricular methylene blue injection > for the > validation of drug/electrode injection/placement accuracy? > > Could you please give me the catalogue number of methylene blue, > so I can > order for myself? And any other advices would be greatly appreciated. > > Thank you very much in advance. > > Salim > > syinan@ucalgary.ca > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jhaviland <@t> mdanderson.org Wed Jul 8 11:20:00 2009 From: jhaviland <@t> mdanderson.org (Haviland,Joie) Date: Wed Jul 8 11:21:07 2009 Subject: [Histonet] CD168 (2D6) antibody Message-ID: Hello... I am attempting to use this antibody, CD168 (2D6), also known as Rhamm or Hmmr. I tried using 10mM citrate buffer but that did not work at all. I did not see any signal. I also have tried using the 1mMEDTA antigen retrieval solution but the control tissue, normal human testis, was no longer there after AR is done. I have tried other control tissues still no results. It was suggested to me to use Excel slides which I did and the tissue stayed but was very degraded after AR. I am using a 1:50 and 1:100 dilution of this antibody but I see a bit of cytoplasm lighting up at 1:50. The data sheet says that it should show on the cell membrane. But the signal only showed up after I left the antibody overnight at 4 degrees. I have also tried the antibody at room temp for 1 hour to no avail. I am using DAKO products, protien block, Mouse Polymer and DAB+ which have worked in other IHC's recently done. I have tried two different lots of this antibody with no success. Any suggestions? Thanks Joie -- Joie Haviland, HT (ASCP) Senior Research Assistant, Dr. McDonnell's Lab Y5.6057 Department of Hematopathology MD Anderson Cancer Research Center 713-792-0705 From jkiernan <@t> uwo.ca Wed Jul 8 11:25:10 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jul 8 11:25:14 2009 Subject: [Histonet] Silver Nitrate Message-ID: Dear Andi, For staining I'm sure it will make no difference which type of silver nitrate you use. This compound is available at 99.9999% purity for analytical purposes! My experience of silver staining has been mostly with nervous tissue, for which the ACS reagent grade (99%) is fine. If a silver stain goes wrong, it's very unlikely to be the fault of the AgNO3. Solutions of silver nitrate (in distilled or deionized water) can be kept for years in brown glass bottles, and can be re-used many times. filter out any bits of sections before returning the solution to its bottle. If a solution of silver nitrate has a greyish tinge it should be replaced; such a solution is likely to deposit and amplify "dirt". Be careful not to contaminate silver nitrate with anything containing chloride (that includes tap water), phosphate or carbonate ions. These all form insoluble light-sensitive silver salts and will spoil a solution of silver nitrate. Silver nitrate is ridiculously expensive and needs to be looked after. John A. Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada = = = ----- Original Message ----- From: Andrea Grantham Date: Wednesday, July 8, 2009 12:00 Subject: [Histonet] Silver Nitrate To: HISTONET > After a recent run on silver stains here I need to order > Silver > Nitrate. Can anyone tell me what the difference is (except the > cost) > between Silver Nitrate > Certified ACS, USP, p.a., Extra Pure, and Ultra Pure? They all > have > the same chemical formula and FW of 169.87. My last batch of > Silver > Nitrate was probably ordered and received here just after > the > Mayflower landed and there is no information on the bottle any > longer. > Which one do I need for stains like GMS, Sevier Munger, etc.? > > Thanx, > > Andi > 8-) > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cell Biology and Anatomy > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > "happy slicing and dicing and may all your stains work > perfectly" - > Paula Sicurello > P Please consider the environment before printing this email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Wed Jul 8 11:49:06 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Jul 8 11:49:10 2009 Subject: [Histonet] ventana IHC and special stains users In-Reply-To: <0A6807B81C7F42BC8516ECBFC86A91D9@prueggihctechlt> Message-ID: Our IHC waste is hauled away like our Alcohol/Xylene sub. The town we are in would not allow the IHC waste down the sink, but they do allow us to throw the NexES sp. Stainer waste down the sink. Maryland is the state. Joyce Cline Technical Specialist -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, July 07, 2009 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventana IHC and special stains users Please for those of you using a Ventana IHC instrument and/or special stains instrument, I am interested in what you do to dispose of your toxic waste. I know each municipality is different so let me know what you do and where you live. Ventana does not separate toxic from non toxic waste so I imagine there is a lot of toxic waste to deal with and the liquid coverslip alone is something that I would think should not go down the drain?????? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Maria.Katleba <@t> stjoe.org Wed Jul 8 12:23:40 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Jul 8 12:23:54 2009 Subject: [Histonet] ventana IHC and special stains users In-Reply-To: References: <0A6807B81C7F42BC8516ECBFC86A91D9@prueggihctechlt> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D056374E@SJSNT-SCMAIL03.stjoe.org> Be careful... Nexus waste DOES contain "silver" waste if you run a GMS, Steiner, etc.... and therefore, as a heavy metal, never go down any drain... no matter what state you live in. I am almost sure it's a federal thing... "waste and waterways" or something like that. Maria Katleba HT(ASCP), MS Pathology Dept. Mgr Queen of the Valley Medical Center Napa, Ca 94558 707-252-4411 x3689 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Wednesday, July 08, 2009 9:49 AM To: Histonet Subject: RE: [Histonet] ventana IHC and special stains users Our IHC waste is hauled away like our Alcohol/Xylene sub. The town we are in would not allow the IHC waste down the sink, but they do allow us to throw the NexES sp. Stainer waste down the sink. Maryland is the state. Joyce Cline Technical Specialist -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, July 07, 2009 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventana IHC and special stains users Please for those of you using a Ventana IHC instrument and/or special stains instrument, I am interested in what you do to dispose of your toxic waste. I know each municipality is different so let me know what you do and where you live. Ventana does not separate toxic from non toxic waste so I imagine there is a lot of toxic waste to deal with and the liquid coverslip alone is something that I would think should not go down the drain?????? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From beth.doran <@t> novartis.com Wed Jul 8 13:24:53 2009 From: beth.doran <@t> novartis.com (beth.doran@novartis.com) Date: Wed Jul 8 13:24:59 2009 Subject: [Histonet] IHC on glutaraldehyde fixed paraffin embedded tissue Message-ID: Hi, I have some tissue that was fixed in glutaraldehyde for 5 days then formalin for another 5 days before being processed into paraffin blocks. I've been asked to do IHC on these samples and I anticipate some difficulty due to the long and strong fixation. Does anyone have any experience with this? Thanks, Beth From lpaveli1 <@t> hurleymc.com Wed Jul 8 14:00:01 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jul 8 14:00:16 2009 Subject: [Histonet] technical charge Message-ID: <4A54B471020000EE0002A98B@smtp-gw.hurleymc.com> I would ask Rene Buesa. He wrote a good article concerning pricing for this very thing. rjbuesa@yahoo.com Lynette >>> 07/08/09 11:05 AM >>> Hello, Does anyone know what is a reasonable charge (technical only ) for an unstained cut as well as per slide for cutting staining and mounting? Thanks Nirmala Srishan __________________ Can you do heat retrieval on frozens? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 8 15:14:20 2009 From: rjbuesa <@t> yahoo.com (Rene J. Buesa) Date: Wed Jul 8 14:13:34 2009 Subject: [Histonet] technical charge Message-ID: <0fbb1dbfb9001f9eca36c67034161d48@fakesend.com> Or alternatively, you could check the archives. ref. EAP1305059 From MElliott <@t> mrl.ubc.ca Wed Jul 8 14:42:49 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Wed Jul 8 14:43:29 2009 Subject: [Histonet] M! and M2 macrophages Message-ID: <4A549449.11C6.00D6.0@mrl.ubc.ca> I have been asked if there are antibodies/markers available for M1 and M2 macrophages that would work on human tissue, either frozen or fixed. Anyone know of any. I checked Biocompare and came up blank. Thanks Mark M1 macrophage A macrophage subtype that produces pro-inflammatory cytokines and acts as an effector of cell killing. M2 macrophage A macrophage subtype that acts to dampen inflammatory responses and scavenge debris, as well as promote angiogenesis and tissue remodelling and repair. ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Margaret.Perry <@t> sdstate.edu Wed Jul 8 14:46:09 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Jul 8 14:46:16 2009 Subject: [Histonet] Mycobacterium bovis Message-ID: I have been looking for a replacement for my antibody from DAKO. It has been discontinued. I've tried the internet and several antibody search engines with no luck. Can anyone point me in the right direction. I am doing IHC paraffin FFPE Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From Heather.D.Renko <@t> osfhealthcare.org Wed Jul 8 14:56:34 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Jul 8 14:56:49 2009 Subject: [Histonet] re: MDR-Acute Leukemia Message-ID: I had an inquiry from an oncologist for a test known as MDR? It is a therapeutic drug marker genetic test I believe for treatment of acute Leukemia, and who performs this type of test would be very helpful. Thank you in advance! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Rcartun <@t> harthosp.org Wed Jul 8 15:35:52 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 8 15:36:00 2009 Subject: [Histonet] Mycobacterium bovis In-Reply-To: References: Message-ID: <4A54CAE8.7400.0077.1@harthosp.org> Check with Virostat in Portland, ME. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Perry, Margaret" 7/8/2009 3:46 PM >>> I have been looking for a replacement for my antibody from DAKO. It has been discontinued. I've tried the internet and several antibody search engines with no luck. Can anyone point me in the right direction. I am doing IHC paraffin FFPE Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Wed Jul 8 15:58:08 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Jul 8 15:58:16 2009 Subject: [Histonet] OSCAR Antibody In-Reply-To: References: <0A6807B81C7F42BC8516ECBFC86A91D9@prueggihctechlt> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D056397B@SJSNT-SCMAIL03.stjoe.org> Can anyone tell me where I can get a really good OSCAR antibody that works with Ventana Ultraview detection kits? I need a pre-dilute if possible. Thanks, Maria Katleba HT(ASCP) MS Pathology Dept. Mgr Queen of the Valley Medical Center Napa CA 94558 707-257-4076 Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From tjasper <@t> copc.net Wed Jul 8 16:35:18 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Jul 8 16:35:24 2009 Subject: [Histonet] OSCAR Antibody References: <0A6807B81C7F42BC8516ECBFC86A91D9@prueggihctechlt> <97C02552ECB11346877D3E83CF833ABD13D056397B@SJSNT-SCMAIL03.stjoe.org> Message-ID: <90354A475B420441B2A0396E5008D4965E305D@copc-sbs.COPC.local> Hi Maria, We run OSCAR on Ventana Ultraview - CC1 Mild, 37 degrees C, 16 minute incubation. We get the antibody from Covance - product #SIG 3465-16. This comes as a 6ml predilute. Our docs like it quite a bit, and order it on a regular basis. Hope this helps. Regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Wednesday, July 08, 2009 1:58 PM To: Histonet Subject: [Histonet] OSCAR Antibody Can anyone tell me where I can get a really good OSCAR antibody that works with Ventana Ultraview detection kits? I need a pre-dilute if possible. Thanks, Maria Katleba HT(ASCP) MS Pathology Dept. Mgr Queen of the Valley Medical Center Napa CA 94558 707-257-4076 Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chana.de.wolf <@t> gmail.com Wed Jul 8 17:29:24 2009 From: chana.de.wolf <@t> gmail.com (Chana de Wolf) Date: Wed Jul 8 17:29:30 2009 Subject: [Histonet] methylene blue microinjection In-Reply-To: References: Message-ID: <3f4b71f10907081529x798dbec0wb09252de5e0d95da@mail.gmail.com> John, It is unfortunate, but common, for researchers to refer to sharp micropipettes as "electrodes." This is probably because they are frequently placed over electrodes in for stimulation and recording. I think Salim probably means that he/she is simply using a micropipette for drug injection (though he/she didn't mention where, and it could very well be a nucleus rather than a ventricle). I have frequently used alcian blue for determining accurate placement of pipettes for microinjection, either in ventricles or nuclei. I can vouch from experience that this method works excellently, at least in lateral ventricle (LV) and paraventricular nucleus (PVN). To determine *electrode* placement, I run current through the electrode to create a visible lesion. Sincerely, Chana de Wolf On Wed, Jul 8, 2009 at 9:03 AM, John Kiernan wrote: > Dear Salim Yalcin Inan, > > You need to explain your question! Electrodes are not ordinarily positioned > to stimulate or record from the CSF in the ventricles of the brain. > Methylene blue is soluble in water (see the Merck Index, Conn's Biological > Stains or any other book about dyes or stains published since about 1885). > How could injecting a soluble dye into one of the ventricles of the brain > lead to "validation of drug/electrode injection/placement accuracy"? > > Methylene blue is listed in the catalogues of almost all vendors of > chemicals because this dye has many uses. A Google Scholar search for > "methylene blue" brings up "about 294,000 hits". You won't get the answers > you need without doing a proper survey of earlier published literature > (peer-reviewed papers, scholarly books etc) targeted at the questions you > are trying to answer. > > Your email address shows that you are at a major Canadian university, but > you don't declare yourself as a professor, postdoc, technician, graduate > student, summer student, janitor's brush carrier or senior administrator. > When asking for help you need to say who you are, as well as explain clearly > what information you need. A well prepared question usually draws several > answers that are helpful also to other people reading the listserver. > > John A. Kiernan > Department of Anatomy & Cell Biology > The University of Western Ontario > London, Canada > = = = > ----- Original Message ----- > From: Salim Yalcin Inan > Date: Tuesday, July 7, 2009 16:52 > Subject: [Histonet] methylene blue microinjection > To: Histonet > > > Dear All, > > > > > > > > Does anyone use intracerebroventricular methylene blue injection > > for the > > validation of drug/electrode injection/placement accuracy? > > > > Could you please give me the catalogue number of methylene blue, > > so I can > > order for myself? And any other advices would be greatly appreciated. > > > > Thank you very much in advance. > > > > Salim > > > > syinan@ucalgary.ca > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TMcNemar <@t> lmhealth.org Thu Jul 9 05:19:05 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jul 9 05:19:26 2009 Subject: [Histonet] slide labeling systems Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E104@lmhsmail.lmhealth.org> I am looking for a slide/block labeling system. I am particularly interested in those that will interface with Meditech and was wondering what people are using and what they like/don't like about them. Is anyone using the Thermo Slidemate? I was talking with the rep yesterday and it seems that it may just the ticket. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From laurie.colbert <@t> huntingtonhospital.com Thu Jul 9 08:58:23 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jul 9 08:58:30 2009 Subject: [Histonet] slide labeling systems Message-ID: <57BE698966D5C54EAE8612E8941D768306088C95@EXCHANGE3.huntingtonhospital.com> Tom, We use the ThermoFisher Printmate for our cassettes, and the PSLIM from Accuplace - now being marketed as the Slidemate by ThermoFisher - for our slides. We have had issues with both, and hopefully we have all of the bugs worked out. We print a 2D barcode on the cassette, which causes the printing to take longer. But the nice thing is, we then read that barcode on the cassette to print out our slide. We no longer have to put paper labels on the slides. We are not interfaced with any LIS at the moment. I'm pretty happy with both. Feel free to call me or email if you have any specific questions. Laurie Colbert Huntington Hospital (626) 397-8620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, July 09, 2009 3:19 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slide labeling systems I am looking for a slide/block labeling system. I am particularly interested in those that will interface with Meditech and was wondering what people are using and what they like/don't like about them. Is anyone using the Thermo Slidemate? I was talking with the rep yesterday and it seems that it may just the ticket. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Farnsworth <@t> cls.ab.ca Thu Jul 9 09:27:14 2009 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Thu Jul 9 09:27:20 2009 Subject: [Histonet] dictation systems Message-ID: Hi all! Anyone out there have any recommendations for dictation systems? I have the rare and exciting opportunity to help plan a new lab within a new hospital and we'd like to consider a new dictation system. We'd need something that would work for grossing, autopsy, pathologists and (possibly remote) access for transcriptionists. We'd prefer a hands free system for grossing and autopsy at least. We're leaning away from voice recognition for various reasons. We're open to telephone systems or computer systems. Any suggestions? VENDORS WELCOME! Thanks in advance, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From EShoreLonelyOne <@t> aol.com Thu Jul 9 09:29:48 2009 From: EShoreLonelyOne <@t> aol.com (EShoreLonelyOne@aol.com) Date: Thu Jul 9 09:30:07 2009 Subject: [Histonet] Tissue Tek Auto-Tec Embedding Station by Sakuara Message-ID: I'm wondering if anyone is currently using or had demonstration of the Tissue Tek Auto-Tec Embedding Station. Currently, I have a few questions: 1. Quality 2. Time saving 3. Cost effectiveness 4. Any problems with small specimens (derms, GI's, cervical biopsies, and non-GYN's) 5. Technicians opinions Thank you for your help. Jay **************Looking for love this summer? Find it now on AOL Personals. (http://personals.aol.com/?ncid=emlcntuslove00000003) From CareerStudio <@t> aol.com Thu Jul 9 10:58:45 2009 From: CareerStudio <@t> aol.com (CareerStudio@aol.com) Date: Thu Jul 9 10:58:53 2009 Subject: [Histonet] Manager, Anatomic Pathology / Histology for Connecticut Lab Message-ID: Our client, a premier, full-service clinical laboratory, is currently seeking a Manager, Anatomic Pathology/Histology to be accountable for overall operations of the Histology Lab and AP Support office. Reporting to the Director of Hospital Operations, this individual will use their ability to motivate a staff to insure that high quality, cost effective services are delivered and will be accountable for hiring, training, goal setting, prioritization, budgets, quality and process improvement, instrument evaluation and customer service. This position reqauires BS degree, HTL preferred, 5+ years experience in high volume Anatomic Pathology laboratory. Excellent computer skills along with leadership ability, problem solving and resolution, teamwork, good communication skills. Must be flexible and adaptable to change. Full time, 1st shift, Mon-Fri, may necessitate occasional off shift/weekend/holidays. Competitive salary, bonus eligibility, relocation asistance to fine city in Connecticut is offered. Please contact Barbara Siegel at careerstudio@aol.com for more information. Career Studio national search Palm Beach, FL careerstudio@aol.com 561-738-6363 http://www.linkedin.com/in/careerstudio ************** Looking for love this summer? Find it now on AOL Personals. (http://personals.aol.com/?ncid=emlcntuslove00000003) From TGoins <@t> mt.gov Thu Jul 9 11:32:38 2009 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jul 9 11:32:43 2009 Subject: [Histonet] Hematoxylin Quality Message-ID: <764D82B61A7B8141AC0659805358A21A133B4770BE@doaisd05220.state.mt.ads> Our lab is currently considering a swich from Richard-Allan Hematoxylin 1 (at $154 per liter) to PremiumLine Hematoxylin (at $30.00 per liter). The pathologists actually prefer the staining results from the cheaper source, but are concerned about longevity - we are required to store the slides for up to 10 years. Is anyone aware of any specific problems associated with the use of PremiumLine Hematoxylin? Thanks, Tresa From kmilne <@t> bccancer.bc.ca Thu Jul 9 12:16:02 2009 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Jul 9 12:16:31 2009 Subject: [Histonet] RE: M1 and M2 macrophages In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC0657807BBEEE3@srvex03.phsabc.ehcnet.ca> Hey Mark, It's hard to get markers that are 100% specific as a lot of them pop up on other cells so you'd probably need to use a combination or markers or serial sections. M1 could be s100a8/9 aka calgranulin A/B but this can also be expressed in tumor epithelium, particularly in breast and a few other types. Apparently neutrophils too. I brought one in but I didn't really see many M1 macs in our tumors (ovarian). M2 could use CD204 or CD206, again, not 100% specific but more likely on M2 than M1. CD163 may possibly work with the same caveats. These can all apparently show up on DCs too so you'd have to look at morphology. So, like most markers, nothing is completely specific but they could help. Katy Message: 5 Date: Wed, 08 Jul 2009 12:42:49 -0700 From: "Mark Elliott" Subject: [Histonet] M! and M2 macrophages To: Message-ID: <4A549449.11C6.00D6.0@mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII I have been asked if there are antibodies/markers available for M1 and M2 macrophages that would work on human tissue, either frozen or fixed. Anyone know of any. I checked Biocompare and came up blank. Thanks Mark M1 macrophage A macrophage subtype that produces pro-inflammatory cytokines and acts as an effector of cell killing. M2 macrophage A macrophage subtype that acts to dampen inflammatory responses and scavenge debris, as well as promote angiogenesis and tissue remodelling and repair. From Margaret.Perry <@t> sdstate.edu Thu Jul 9 14:22:39 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Jul 9 14:23:10 2009 Subject: [Histonet] time in formula 83 Message-ID: Our processor has gone down but we can process up to the paraffin step. My question is how long can we keep the tissues in the formula 83 without any visible problems? We would like to start processing tonight and possibly infiltrate by hand tomorrow. Not optimal but it does work. Your comments are gratefully appreciated. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From jclark <@t> pcnm.com Thu Jul 9 17:39:58 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Jul 9 17:40:05 2009 Subject: [Histonet] DAKO Liquid DAB Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C0105C3B2@mail.pcnm.com> DAKO is discontinuing its product 'Liquid DAB' and wants its clients to use the Liquid DAB+ in its place. I have tried this product before and feel it gives too strong a signal. To use it I will have to re-do all my protocols and hence revalidate all my IHC. Can anyone suggest another supplier that has a liquid DAB that is similar in strength to the DAKO product? Thanks for you input. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From Rcartun <@t> harthosp.org Thu Jul 9 19:13:26 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jul 9 19:13:33 2009 Subject: [Histonet] ER/PR HER2 Redux In-Reply-To: <4A51F013.5D38.00EF.0@swmail.sw.org> References: <4A51F013.5D38.00EF.0@swmail.sw.org> Message-ID: <4A564F65.7400.0077.0@harthosp.org> It is difficult for many Anatomic Pathology Laboratories to document the exact time in formalin for breast specimens. Here at Hartford Hospital, we have PAs, Residents, Surgical Pathology Fellows, and Pathologists doing the gross dissection on breast specimens throughout the day in different locations. In addition, our tissue processors start at different times. We demand that our interventional radiologists and our breast surgeons document on the pathology requisition the "formalin contact time" when they place the specimen into formalin. We take the responsibility of documenting the "formalin contact time" when the specimen is received fresh here in Pathology. If this is not done, I contact the responsible party and inform them that we have a "Biospecimen deficiency" and tell them that they must always document the "formalin contact time" for breast specimens. I think in the near future we will be doing this for most cancer specimens that we see in our laboratories. Our tissue processors have 4 hours of formalin on them for large specimens like breast excisions. The majority of our processors are started around 5:30 p.m. every evening. Therefore, we have a policy in place that states that breast specimens (not needle core biopsies) must be in formalin prior to 3:30 p.m.; if not, they are held until the next day. This allows at least 6 hours of formalin fixation (ASCO/CAP guideline) for all large breast specimens. For smaller breast specimens like needle cores and mammotome biopsies, I have validated that 4.5 hours is sufficient for reliable immunohistochemical and molecular testing. I don't believe that it is necessary to document the "total fixation time" on the pathology report. You need to develop a policy that ensures that the 2007 ASCO/CAP guidelines are followed or you can choose to do your own validation of fixation times on-site. Finally, please keep in mind that fixation time is only one piece to the puzzle. Minimizing ischemic time, making sure that the tissue does not dry-out, and taking "thin" (2 mm) tissue sections for processing are probably equally important, if not more. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Nita Searcy" 7/6/2009 1:37 PM >>> Funny, I thought I had this issue taken care of but we are once again discussing it! What are others doing to verify formalin fixation times? Is it "good enough" to have a blanket statement in the report that we are in compliance of the 6-48 hour required time ( we have fixation time on container dictated into report & we process on the weekend ) or is the actual hours of fixation ( time in formalin container+ time in processor = total fixation time) documented into the report? And do you document ALL? So that even those that are unsuspected (reductions) get a fixation ( just in case). Discussion on Wednesday. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 From histosearch <@t> gmail.com Fri Jul 10 08:43:32 2009 From: histosearch <@t> gmail.com (Matthew Semovoski) Date: Fri Jul 10 08:43:37 2009 Subject: [Histonet] Histology Supplies Message-ID: <521c6d260907100643v41ee312aude0c862935d81e1e@mail.gmail.com> Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com. I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com From LRaff <@t> uropartners.com Fri Jul 10 10:38:28 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Jul 10 10:38:33 2009 Subject: [Histonet] New CAP question on records and anatomic retention Message-ID: Hello All: Just got our self inspection material and came across this new question dealing with ending lab operations. Has anyone, especially independent labs or hospitals not part of a big system, developed such a policy? Gen.20425 Does the laboratory have a policy to ensure that all records, slides, blocks and tissues are retained and available for appropriate times should the laboratory cease operations. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 From Tracey.Lenek <@t> cls.ab.ca Fri Jul 10 11:51:36 2009 From: Tracey.Lenek <@t> cls.ab.ca (Tracey Lenek) Date: Fri Jul 10 11:51:41 2009 Subject: [Histonet] HSV Controls Message-ID: <2DFAEEFF192A9141ABACFF88BE613BF33A1DDA9F7F@EXMBXC1.crha.bewell.ca> Hi, Does anyone know of a source for HSV I/II positive paraffin control blocks? Thanks in advance. Tracey Lenek Temporary Supervisor - Anatomic Pathology Calgary Laboratory Services 403-770-3448 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From sbreeden <@t> nmda.nmsu.edu Fri Jul 10 14:08:26 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jul 10 14:08:31 2009 Subject: [Histonet] An SOP on Tissues, Slides and/or Wet Tissue? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46992@nmdamailsvr.nmda.ad.nmsu.edu> Re-inventing the wheel is so pass? - I need to write a pretty descriptive SOP on our policy of not releasing slides, blocks and (sometimes) wet tissue to other parties (keeping in mind we are a veterinary diagnostic and "reference" lab). The State policy for records (it does not specifically mention that these "records" must be paper) can be widely interpreted to include slides, blocks and (sometimes) wet tissue as being part of the records that must be kept, depending upon the phase of the moon and the interpreter. I convinced my pathologists some time back that these materials are the property of the State once they are accessioned but I need to morph that into a written policy so I have at least one leg on which to plant myself. To make a long story short (too late!), is there another vet lab out there that already HAS a written SOP/policy on the release of original material (wet tissue, blocks, slides) that I might be able to use as a format for writing one? I believe this might qualify as a Friday Moment of Fuming if I think about it long enough. Other than that, life is good and I have no specific annoyances or pet peeves - at this moment! May your weekend be four days long and your workload when you return be a light one! By the way, it's awfully QUIET out there in Histonetland - are we all that busy? Yours in paraffin... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From ploykasek <@t> phenopath.com Fri Jul 10 14:38:10 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Jul 10 14:38:23 2009 Subject: [Histonet] Bone marrows Message-ID: Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From gu.lang <@t> gmx.at Fri Jul 10 15:25:59 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jul 10 15:26:06 2009 Subject: AW: [Histonet] Bone marrows In-Reply-To: References: Message-ID: <1857C8B2846E4035AD245ED1734C8BAA@dielangs.at> BM biopsies of 3mm diameter, Overnight fixation in NBF, 6-8 hours decal in a commercial mixture of formic acid and formaldehyd (about 5-10%, Richard Allen Scientific). Routine paraffin embedding. Good IHC results, Kappa-Lambda ISH possible. Gudrun Lang Histolab Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patti Loykasek Gesendet: Freitag, 10. Juli 2009 21:38 An: histonet Betreff: [Histonet] Bone marrows Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jul 10 16:16:59 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 10 16:17:04 2009 Subject: [Histonet] Bone marrows In-Reply-To: References: Message-ID: Patti, I prefer formic acid decal for IHC work. You could make your own 5% but I always buy it from Decal Chemicals it is called Immunocal. I have found the rapid decal solutions are usually nitric acid and not good for IHC. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Friday, July 10, 2009 1:38 PM To: histonet Subject: [Histonet] Bone marrows Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jul 10 16:18:45 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 10 16:18:48 2009 Subject: [Histonet] Histology Supplies In-Reply-To: <521c6d260907100643v41ee312aude0c862935d81e1e@mail.gmail.com> References: <521c6d260907100643v41ee312aude0c862935d81e1e@mail.gmail.com> Message-ID: I would like to know about their charged slides as well, I told my techs to ask for samples to try before we buy but I do not think they have done that yet. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Semovoski Sent: Friday, July 10, 2009 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Supplies Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com. I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jul 10 16:23:31 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 10 16:23:39 2009 Subject: [Histonet] DAKO Liquid DAB In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C0105C3B2@mail.pcnm.com> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C0105C3B2@mail.pcnm.com> Message-ID: <4C4906DCDE5C44DDAFFB64454E0C64A4@prueggihctechlt> I use Stable Dab from Open Biosystems, no mixing, you keep it in a freezer -20 but it does not freeze, it has methanol or glycerine or something in it that keeps it from freezing, you should not freeze DAB solutions. I love it and it is a lot cheaper than using Dako or anyone else. I also buy antibody diluent, protein block and digestion reagents from Open Biosystems. Their website and catalogs do not list these reagents so you have to call and ask for them. They make a lot of products for molecular work, probes etc. I think this IHC stuff is sort of a side line for them. These reagents were first developed by David Bragatti who is now passed. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Thursday, July 09, 2009 4:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Liquid DAB DAKO is discontinuing its product 'Liquid DAB' and wants its clients to use the Liquid DAB+ in its place. I have tried this product before and feel it gives too strong a signal. To use it I will have to re-do all my protocols and hence revalidate all my IHC. Can anyone suggest another supplier that has a liquid DAB that is similar in strength to the DAKO product? Thanks for you input. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srnty <@t> aol.com Fri Jul 10 18:13:22 2009 From: srnty <@t> aol.com (srnty@aol.com) Date: Fri Jul 10 18:14:09 2009 Subject: [Histonet] Cassette Blues Message-ID: <8CBCFCB276D2B3E-1770-1AB2@webmail-db10.sysops.aol.com> This list has been invaluable to me since returning part-time to histology after an 8 year hiatus. Thank you. Here's my latest dilemma. When I left the last job, I swear the holes in the plastic tissue cassettes were larger. I got to the point where I could embed and barely have to scrape any paraffin off the block before cutting. I am making the biggest mess with these newer cassettes. They are slotted. First of all, I get air bubbles in the blocks and the tissue has to be re-embedded. I may have solved this problem by tapping on the top of the cassette. If anyone has another method, would you kindly share it with me? Secondly, there is so much paraffin on the outside of the block, I have to scrape and scrape and scrape. Any one out there able to embed neatly with these and if so what's your method? Many thanks! Marg From jkiernan <@t> uwo.ca Sat Jul 11 01:46:10 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jul 11 01:46:15 2009 Subject: [Histonet] Bone marrows Message-ID: Patsy Ruegg pruegg@ihctech.net wrote: > I prefer formic acid decal for IHC work. You could make > your own 5% but I > always buy it from Decal Chemicals it is called Immunocal. Why? Does it cost you less to buy pre-diluted formic acid? John Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada = = = From tgenade <@t> gmail.com Sat Jul 11 08:11:53 2009 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sat Jul 11 08:11:59 2009 Subject: [Histonet] anyone using Promega TUNEL assay? Message-ID: Hello, For my PhD project my Prof and I are entertaining doing some TUNEL work on our brain sections in search of apoptotic nuclei but the kit is very expensive and would like to get some more information from some one who is using one of the kits and doesn't have a financial interest in selling us one. Locally, the Promega Kit has the highest tests to cost ratio. Anyone out there using this kit and willing to share their experience? -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From carl.hobbs <@t> kcl.ac.uk Sat Jul 11 14:06:52 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Jul 11 14:08:29 2009 Subject: [Histonet] DAKO Liquid DAB Message-ID: <11D9615B89C10747B1C985966A63D7CA2977449102@KCL-MAIL04.kclad.ds.kcl.ac.uk> I use a homemade DAB solution: I aliquot and freeze. No problemo for 15 yrs, Patsy. If you do not agree , please check out this site http://www.immunoportal.com/index.php All of my DAB images are developed using -20C DAB aliquots. carlos From CarterK <@t> MedImmune.com Sat Jul 11 16:11:01 2009 From: CarterK <@t> MedImmune.com (Carter, Kendra) Date: Sat Jul 11 16:12:02 2009 Subject: [Histonet] Parathyroid Tissue References: Message-ID: <1D2ACDFBBD4EA043A10496D860743B92F3352B@MD1EV001.medimmune.com> Does anyone know where I can buy snap frozen normal parathyroid tissue? Kendra Leigh Carter GLP Documentation & Archive Specialist MedImmune One MedImmune Way Gaithersburg, MD 20878 Tel: 301-398-4956 Fax: 301-398-9956 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From amosbrooks <@t> gmail.com Sun Jul 12 19:04:18 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jul 12 19:04:23 2009 Subject: [Histonet] anyone using Promega TUNEL assay? Message-ID: <582736990907121704n350a4019nb57f4f67aec1dd5f@mail.gmail.com> Hi Tyrone, OK so this is probably no help regaurding the Promega kit, because I've never used it. I have used the Chemicon Apoptag kit and can't reccommend it enough. Not the easiest test I've ever done, but it is one of the few chromogenic kits out there. It is very clean & reliable. Amos Message: 9 Date: Sat, 11 Jul 2009 15:11:53 +0200 From: Tyrone Genade Subject: [Histonet] anyone using Promega TUNEL assay? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, For my PhD project my Prof and I are entertaining doing some TUNEL work on our brain sections in search of apoptotic nuclei but the kit is very expensive and would like to get some more information from some one who is using one of the kits and doesn't have a financial interest in selling us one. Locally, the Promega Kit has the highest tests to cost ratio. Anyone out there using this kit and willing to share their experience? -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) From Jason.PALMER <@t> svhm.org.au Sun Jul 12 20:05:00 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Sun Jul 12 20:05:16 2009 Subject: [Histonet] RE: promega tunel Message-ID: I have used a Promega Dead-End kit for a few years now and am happy enough with the results - get consistent TUNEL labelling with a biotin / DAB kit from run to run, and get away with using smaller volumes than they suggest, with coverslips for the reaction mix incubation. The only thing is (and I think this is true for TUNEL in general) that interpretation can be tricky as there seem to be a lot of false positive cells, especially the longer you do your proteinase K digestion for. (Negative controls are clean.) So you may need to confirm what you get morphologically (not always easy, depending on your experience and the cells you are looking at) or by some other method - I am trying to work up cleaved caspase 3 immunostaining at the moment for just this purpose. Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Message: 9 Date: Sat, 11 Jul 2009 15:11:53 +0200 From: Tyrone Genade Subject: [Histonet] anyone using Promega TUNEL assay? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, For my PhD project my Prof and I are entertaining doing some TUNEL work on our brain sections in search of apoptotic nuclei but the kit is very expensive and would like to get some more information from some one who is using one of the kits and doesn't have a financial interest in selling us one. Locally, the Promega Kit has the highest tests to cost ratio. Anyone out there using this kit and willing to share their experience? -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 11 **************************************** ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. 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For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From koellingr <@t> comcast.net Sun Jul 12 22:08:38 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Jul 12 22:08:41 2009 Subject: [Histonet] anyone using Promega TUNEL assay? In-Reply-To: Message-ID: <1841337155.476211247454518700.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Tyrone, I agree with Amos Brooks about the Chemicon kit and I agree with Jason Palmer about about Promega, especially?in regards to smaller, controlled?volumes to decease cost per slide.? In addition, I only used?both those kits directions as starting points.? Took care of?the proteinase digestion optimized to my standards for my controls in my lab and also emperically found a?lesser amount (much less than suggested) of Tdt was preferable.? In fact, I could attribute false-positive staining to use of kit suggested amounts of Tdt.? Backing off on that concentration eliminated false positives while retaining strong pos staining. And made kit last longer. As for working up caspace-3, I'm sure you aware of the caveat that (1) brain especially?can utilize?non-canonical apoptosis pathways (caspace-3 independent pathways that leads to false negatives in the assay) and (2) there is now clear and convincing evidence that caspace-3 activity in brain has been linked to other things aside from apoptosis such as synaptic plasticity modulation and other non-apoptotic but normal brain functions. Ray Koelling PhenoPath Labs Seattle, WA? ----- Original Message ----- From: "Tyrone Genade" To: "histonet" Sent: Saturday, July 11, 2009 6:11:53 AM GMT -08:00 US/Canada Pacific Subject: [Histonet] anyone using Promega TUNEL assay? Hello, For my PhD project my Prof and I are entertaining doing some TUNEL work on our brain sections in search of apoptotic nuclei but the kit is very expensive and would like to get some more information from some one who is using one of the kits and doesn't have a financial interest in selling us one. Locally, the Promega Kit has the highest tests to cost ratio. Anyone out there using this kit and willing to share their experience? -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yingling <@t> temple.edu Mon Jul 13 09:00:36 2009 From: yingling <@t> temple.edu (VANESSA YINGLING) Date: Mon Jul 13 09:00:40 2009 Subject: [Histonet] unsubscribe Message-ID: HI there, I am trying to unsubscribe from this list but have not been able to. Thanks for your assistance. Vanessa -- Vanessa R. Yingling, Ph.D. FACSM Assistant Professor Temple University Department of Kinesiology College of Health Professions Department of Anatomy and Cell Biology Temple Medical School 1800 North Broad Street Philadelphia, PA 19122 215-204-4471 fax: 215-204-4414 http://www.temple.edu/chp/departments/kinesiology/SkAD_Lab.htm "You must do the thing you think you cannot do" -Eleanor Roosevelt From mpence <@t> grhs.net Mon Jul 13 09:34:34 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jul 13 09:34:42 2009 Subject: [Histonet] Cassette Blues In-Reply-To: <8CBCFCB276D2B3E-1770-1AB2@webmail-db10.sysops.aol.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BAB@is-e2k3.grhs.net> Find a different vendor for your cassettes. There are cassettes out there with bigger holes. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srnty@aol.com Sent: Friday, July 10, 2009 6:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Blues This list has been invaluable to me since returning part-time to histology after an 8 year hiatus. Thank you. Here's my latest dilemma. When I left the last job, I swear the holes in the plastic tissue cassettes were larger. I got to the point where I could embed and barely have to scrape any paraffin off the block before cutting. I am making the biggest mess with these newer cassettes. They are slotted. First of all, I get air bubbles in the blocks and the tissue has to be re-embedded. I may have solved this problem by tapping on the top of the cassette. If anyone has another method, would you kindly share it with me? Secondly, there is so much paraffin on the outside of the block, I have to scrape and scrape and scrape. Any one out there able to embed neatly with these and if so what's your method? Many thanks! Marg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Mon Jul 13 10:16:31 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Jul 13 10:16:35 2009 Subject: [Histonet] (EDTA)Decal Bone marrows Message-ID: <851724.70171.qm@web31308.mail.mud.yahoo.com> Hi Patti, Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a Hematopathologist at NIH.? This formula was particularly great for BM Core BX's.? It is much gentler on the tissue, and is less likely to harm the cellular integrity.? Dr. Todd Barry also prefers the use of EDTA formula's for BM Bx's. Below is the formula. or you can purchase from Biocare Medical. You should decal the specimen for 30 minutes to 1 hour and check for decalcification.? DO NOT leave for any longer than 1 hour. 10% EDTA (Ethyl-media minetetra-acetic-acid Stock EDTA 10 ml DI water?????? 90 ml pH 4.5-5.0 with Sodium Hydroxide If you prefer to purchase the IED Solution, Biocare Medical has it available.? They have 2 sizes 140 ml or 500 ml.? cat # IED 1204. Ion-Exchange Decalcification Unit (IED Unit, Designed for Bone Marrow Biopsies) An advanced decalcification system that removes calcium from bone quickly while leaving superior cellular detail. The IED Unit incorporates a strong cation ion-exchange resin in a weak acid solution to remove calcium ions from bone, replacing them with hydrogen ions. Because the IED Unit does not require strong concentrated acid solutions, as in traditional decalcification methods, delicate cellular structures antigenicity remain intact. Regards, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 E-Mail: aallison-tacha@apmglab.com --- On Fri, 7/10/09, Patti Loykasek wrote: From: Patti Loykasek Subject: [Histonet] Bone marrows To: "histonet" Date: Friday, July 10, 2009, 12:38 PM Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bengoodwin30 <@t> gmail.com Mon Jul 13 10:33:36 2009 From: bengoodwin30 <@t> gmail.com (Benjamin Goodwin) Date: Mon Jul 13 10:33:42 2009 Subject: [Histonet] (no subject) Message-ID: <2243bd220907130833s744e6aa1y64d49ecbcd41babe@mail.gmail.com> Please take me off your list. Thank you. From bengoodwin30 <@t> gmail.com Mon Jul 13 11:47:19 2009 From: bengoodwin30 <@t> gmail.com (Benjamin Goodwin) Date: Mon Jul 13 10:45:25 2009 Subject: [Histonet] (no subject) Message-ID: <15e492bbd8b76c7b9511574f4d61909c@fakesend.com> Please take me off your list. Thank you. ref. EAP1328019 From bengoodwin30 <@t> gmail.com Mon Jul 13 11:47:45 2009 From: bengoodwin30 <@t> gmail.com (Benjamin Goodwin) Date: Mon Jul 13 10:45:50 2009 Subject: [Histonet] (no subject) Message-ID: Please take me off your list. Thank you. ref. EAP1328026 From dlschneider <@t> gmail.com Mon Jul 13 11:08:15 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Mon Jul 13 11:08:18 2009 Subject: [Histonet] Job: Histology Supervisor in Amarillo, Texas Message-ID: <1085e7000907130908k6318bbdk5001aff9f696f4b5@mail.gmail.com> I'm a pathologist in an established group practice in Amarillo, Texas. We're seeking a histology supervisor. Ideally, we're hiring a permanent employee; however, individuals preferring a locum tenens arrangement (for at least one month) should apply as well. The desired candidate should anticipate very competive compensation. We're seeking someone with excellent communication and people skills, as well as the requisite technical expertise. If you're interested, or know someone who might be, please drop me an email and we'll discuss the specifics. Daniel Schneider, MD From sfeher <@t> CMC-NH.ORG Mon Jul 13 11:12:02 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Jul 13 11:12:23 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From dlschneider <@t> gmail.com Mon Jul 13 11:14:23 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Mon Jul 13 11:14:31 2009 Subject: [Histonet] Job: Histotech in Amarillo, Texas Message-ID: <1085e7000907130914s384aa70fj2e75dffea4205a8f@mail.gmail.com> I'm a pathologist in an established group practice in Amarillo, Texas. We're seeking well-trained, experienced histotechs. Ideally, we're hiring a permanent employee; however, individuals preferring a locum tenens arrangement (for at least one month) should apply as well. We're seeking well-trained, experienced histotechs. The desired candidate should anticipate very competive compensation. Individuals interested in a supervisory or management position should refer to my earlier post seeking a Histology Supervisor. If you're interested, or know someone who might be, please drop me an email and we'll discuss the specifics. Daniel Schneider, MD On Mon, Jul 13, 2009 at 11:08 AM, Daniel Schneider wrote: > > I'm a pathologist in an established group practice in Amarillo, Texas. > We're seeking a histology supervisor. Ideally, we're hiring a permanent > employee; however, individuals preferring a locum tenens arrangement (for at > least one month) should apply as well. > > The desired candidate should anticipate very competive compensation. > > We're seeking someone with excellent communication and people skills, as > well as the requisite technical expertise. > > If you're interested, or know someone who might be, please drop me an email > and we'll discuss the specifics. > > Daniel Schneider, MD > > > From lblazek <@t> digestivespecialists.com Mon Jul 13 11:39:37 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jul 13 11:37:31 2009 Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088C7B31B8@IBMB7Exchange.digestivespecialists.com> Why do you have to hire an HTL? Why not an HT ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Mon Jul 13 11:40:31 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Jul 13 11:40:35 2009 Subject: [Histonet] Promega TUNEL assay...Or any TUNEL Assay In-Reply-To: <1841337155.476211247454518700.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <1841337155.476211247454518700.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: I don't like TUNEL on FFPE tissue. Way too many false positives. And yes, I've tried titrating down the concentration of TdT and substrate. Then I found out that formaldehyde causes DNA strand breaks. In other words for your positive control, you can used formalin fixed tissue, because formalin has the same effect as DNAase. The other thing problem with TUNEL on FFPE tissue is that the TUNEL assay detects DNA strand breaks. Any tissue, fixed of frozen, that is on a slide HAS been subjected to another procedure that causes double tranded DNA breaks--a procedure called microtomy. When a microtome bvlade passes through the nucleus of a cell it breaks a lot of DNA strands. And every one of them is detectable by TUNEL. I've heard of people getting rerasonable results with whole cells and frozen tissues, by for FFPE tissue, my current philosophy is: It is an assay that CANNOT work, even in principle. 'Course, I've been wrong about other things, I'm open to persuasion. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Lauren found her dream laptop. Find the PC that?s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290 From Margaret.Perry <@t> sdstate.edu Mon Jul 13 11:49:31 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Jul 13 11:49:37 2009 Subject: [Histonet] Thermo Fishe maintenance contracts Message-ID: Do any of you use Thermo-Fisher for your maintenance contract consolidation? What kind of service do you receive and are the people who do the service competent? Have you had any problems and were they resolved quickly and efficiently? Was it easy to drop and add items from the contract? Please contact me directly with your comments as I would not want any negative comments on this forum. Thank You Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From arvidsonkristen <@t> yahoo.com Mon Jul 13 12:34:45 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Jul 13 12:34:49 2009 Subject: [Histonet] Uranyl Nitrate Message-ID: <418183.74768.qm@web65716.mail.ac4.yahoo.com> Anyone still using Uranyl Nitrate.? We have some in our fridge from '97 and it's very expensive to get rid of.? Any suggestions? From POWELL_SA <@t> mercer.edu Mon Jul 13 12:36:24 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Jul 13 12:37:33 2009 Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE21AB10F16AD@MERCERMAIL.MercerU.local> Those of us who are HTLs without a BS degree were grandfathered in by ASCP when the certification was first offered. As I understand that the fact that we passed the HTL meant that we had the required education and skills (and at that time we had a practical to prove we could do the work) for that certification: the equivalent to a BS degree. To exclude those who do not have the paper BS is like saying we do not count. Thank goodness my employer thinks I do. I do not have any references for you to prove your position but I think you will hear from others who feel the same way. Shirley A. Powell, HT(ASCP)HTL, ASCP Histology professional for 47 years -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Jul 13 12:44:24 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Jul 13 12:44:31 2009 Subject: [Histonet] Uranyl Nitrate In-Reply-To: <418183.74768.qm@web65716.mail.ac4.yahoo.com> References: <418183.74768.qm@web65716.mail.ac4.yahoo.com> Message-ID: <4A5B7278.4030905@umdnj.edu> I'm assuming it's expensive to dispose of due to its low-level radioactivity. I'd recommend precipitating it into a solid uranium salt before disposal... at least you'd have much less of it to dispose of ;) kristen arvidson wrote: > Anyone still using Uranyl Nitrate. We have some in our fridge from '97 and it's very expensive to get rid of. Any suggestions? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mtighe <@t> trudeauinstitute.org Mon Jul 13 12:45:15 2009 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Jul 13 12:45:45 2009 Subject: [Histonet] Antigen retrieval Message-ID: <4A5B3A6B.26E4.00EE.0@trudeauinstitute.org> I am attempting to do IF on FFPE sections but the history/treatment of my tissues (mouse tissues) is unknown. I would like to recommend to our labs a strategy for tissue collection, length of time in NBF, type of antigen retrieval, and so on. Would anyone (or everyone) be willing to share their opinions on the best methods they have for maintaining the ability to do IF or IHC. Thanks for any help! Mike From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jul 13 13:12:43 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jul 13 13:12:48 2009 Subject: [Histonet] Frozen controls Message-ID: <65365F35C0F2EF4D846EC3CA73E49C437AA21CB1C5@HPEMX3.HealthPartners.int> Does anyone run a control for your frozen section staining set-up? If so, how often do you run a control and do you store them in the freezer? Thanks for your answers!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From akemiat3377 <@t> yahoo.com Mon Jul 13 13:29:11 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Jul 13 13:29:14 2009 Subject: [Histonet] Rotary Microtome's and the Meditome Message-ID: <482760.40688.qm@web31307.mail.mud.yahoo.com> Hi everyone in histoland, I am in the market for 3 microtome's.? I was originally thinking of purchasing demo or refurbished Microm 355S units or comparable.? I used this unit when I set-up a TMA lab and was very happy with it.? I am in the process of demo'ing a Leica RM2255 unit.? The Leica unit has all the bells and whistles the Microm microtomes has, but it is on a separate device vs Microm's features are created internally.? I would like your thoughts on this. Also, I was given some information on a NEW Microtome called the Meditome Rotary Microtome.? It is made in Germany by Medite and distributed by Micron, based in San Diego.? It is my understanding that the engineers who made the Reichert Jung and the Microm microtomes developed this new microtome.? It sells for under $10,000 and has a 2 year warranty vs the normal 1 year warranty.? Although it doesn't have all the fancy features, it looks like a solid unit.? Has anyone purchased one of these units, and if so, how do you like it? Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 E-Mail: aallison-tacha@apmglab.com From LINDA.MARGRAF <@t> childrens.com Mon Jul 13 13:40:33 2009 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Jul 13 13:41:01 2009 Subject: [Histonet] misleading message on Histonet last Friday Message-ID: <4A5B3951.F783.00DA.0@childrens.com> Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From Maria.Katleba <@t> stjoe.org Mon Jul 13 13:44:02 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Jul 13 13:44:14 2009 Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39088C7B31B8@IBMB7Exchange.digestivespecialists.com> References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> <5A2BD13465E061429D6455C8D6B40E39088C7B31B8@IBMB7Exchange.digestivespecialists.com> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F51CD9@SJSNT-SCMAIL03.stjoe.org> You would want to hire an HTL if you were going to groom them to be a manager. Wouldn't you want to have a person with a BS at minimum to run the department??? There is something to be said about an educated employee. Also, most hospitals 'require' a BS at minimum for any supervisor or management position. That's how you stay competitive. Experience is great, but getting a degree says a lot about a person, especially in today's economy! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, July 13, 2009 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? Why do you have to hire an HTL? Why not an HT ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Maria.Katleba <@t> stjoe.org Mon Jul 13 13:48:32 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Jul 13 13:48:43 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <4A5B3951.F783.00DA.0@childrens.com> References: <4A5B3951.F783.00DA.0@childrens.com> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F51CE6@SJSNT-SCMAIL03.stjoe.org> OMG... I do not like primates (especially gorillas)... So I probably won't be buying anything from them any time soon. Just kidding. Seriously, I probably would stay away from that kind of a marketing name unless you are appealing to primate research facilities. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Jessica.Snay <@t> mpiresearch.com Mon Jul 13 13:57:48 2009 From: Jessica.Snay <@t> mpiresearch.com (Jessica Snay) Date: Mon Jul 13 13:57:51 2009 Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D0F51CD9@SJSNT-SCMAIL03.stjoe.org> Message-ID: I agree! Jessica Snay, B.S., HTL (ASCP) MPI Research Group Leader, Histology 269-668-3336 x1759, LS-22 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Monday, July 13, 2009 2:44 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? You would want to hire an HTL if you were going to groom them to be a manager. Wouldn't you want to have a person with a BS at minimum to run the department??? There is something to be said about an educated employee. Also, most hospitals 'require' a BS at minimum for any supervisor or management position. That's how you stay competitive. Experience is great, but getting a degree says a lot about a person, especially in today's economy! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, July 13, 2009 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: What percent of HTL's do not have a BS degree? Why do you have to hire an HTL? Why not an HT ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication, including attachments, is for the exclusive use of addressee and may contain proprietary, confidential and/or privileged information. If you are not the intended recipient, any use, copying, disclosure, dissemination or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender immediately by return e-mail, delete this communication and destroy all copies. From mpence <@t> grhs.net Mon Jul 13 14:35:34 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jul 13 14:35:45 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <4A5B3951.F783.00DA.0@childrens.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BAC@is-e2k3.grhs.net> I did not receive this message and have not seen this message come up in the last several weeks. Are you sure this came on the listserv? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Jul 13 14:42:11 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Jul 13 14:42:15 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3BAC@is-e2k3.grhs.net> Message-ID: <1724324211.48461247514131696.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Yes, I got it Friday also.? Pam Marcum ----- Original Message ----- From: "Mike Pence" To: "LINDA MARGRAF" , histonet@lists.utsouthwestern.edu Sent: Monday, July 13, 2009 3:35:34 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] misleading message on Histonet last Friday I did not receive this message and have not seen this message come up in the last several weeks. Are you sure this came on the listserv? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, ?I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com ?I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? ?Thanks, ?Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Mon Jul 13 14:46:33 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jul 13 14:46:39 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3BAC@is-e2k3.grhs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BAD@is-e2k3.grhs.net> I stand corrected. Sorry! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, July 13, 2009 2:36 PM To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] misleading message on Histonet last Friday I did not receive this message and have not seen this message come up in the last several weeks. Are you sure this came on the listserv? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> umn.edu Mon Jul 13 14:47:14 2009 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Mon Jul 13 14:47:15 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3BAC@is-e2k3.grhs.net> References: <4A5B3951.F783.00DA.0@childrens.com> <661949901A768E4F9CC16D8AF8F2838C017A3BAC@is-e2k3.grhs.net> Message-ID: I received the message on Friday At 02:35 PM 7/13/2009, Mike Pence wrote: >I did not receive this message and have not seen this message come up in >the last several weeks. >Are you sure this came on the listserv? > >Mike > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA >MARGRAF >Sent: Monday, July 13, 2009 1:41 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] misleading message on Histonet last Friday > > >Dear Histonetters: >I got the message below privately from one of the list members and (with >their permission) wanted to pass it along to the membership..... > >Dear Linda: >Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this >AM: Hey everyone, I am looking at getting some supplies for a new >histology lab. Has anyone ever purchased from Gorilla Scientific? >www.GorillaScientific.com I just got a flyer in the mail from them and >it seems like they have some really good prices. I think I can save a >lot on my slides if I purchase from them. Has anyone else on here >purchased anything from them? Thanks, Alex histosearch@gmail.com >Well it was not Alex who posted it was Matthew Semovoski who posted this >& he is the man who answers the phone when you call up Gorilla >Scientific. I as a sales person play fair & do not try to do any slick >sales adds on histonet & wanted to make you aware of this. (I really am >such a geek & LOVE TO LEARN from Histonet too!) So I really do want to >thank you for all of the hard work that you do & just wanted to make you >aware of this instance. Thank you so much & have a great weekend! > > >I don't want to turn this into a major discussion but would like to >remind everyone that we run Histonet using University of Texas resources >and they would not appreciate (or support) its use for commercial >advertising even if it is disguised in a misleading message. Thank you, >Linda M Histonet administrator > > > >Please consider the environment before printing this e-mail. > >This e-mail, facsimile, or letter and any files or attachments >transmitted with it contains information that is confidential and >privileged. This information is intended only for the use of the >individual(s) and entity(ies) to whom it is addressed. If you are the >intended recipient, further disclosures are prohibited without proper >authorization. If you are not the intended recipient, any disclosure, >copying, printing, or use of this information is strictly prohibited and >possibly a violation of federal or state law and regulations. If you >have received this information in error, please notify Children's >Medical Center Dallas >immediately at 214-456-4444 or via e-mail at privacy@childrens.com. >Children's Medical Center Dallas and its affiliates hereby claim all >applicable privileges related to this information. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Jul 13 14:56:03 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Jul 13 14:56:14 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <1724324211.48461247514131696.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1724324211.48461247514131696.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <4A5B9153.3060303@umdnj.edu> Me too. Here it is in the archives: http://lists.utsouthwestern.edu/mailman/htdig/histonet/2009-July/045406.html Pamela Marcum wrote: > Yes, I got it Friday also. Pam Marcum > > > > ----- Original Message ----- > From: "Mike Pence" > To: "LINDA MARGRAF" , histonet@lists.utsouthwestern.edu > Sent: Monday, July 13, 2009 3:35:34 PM GMT -05:00 US/Canada Eastern > Subject: RE: [Histonet] misleading message on Histonet last Friday > > I did not receive this message and have not seen this message come up in > the last several weeks. > Are you sure this came on the listserv? > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA > MARGRAF > Sent: Monday, July 13, 2009 1:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] misleading message on Histonet last Friday > > > Dear Histonetters: > I got the message below privately from one of the list members and (with > their permission) wanted to pass it along to the membership..... > > Dear Linda: > Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this > AM: Hey everyone, I am looking at getting some supplies for a new > histology lab. Has anyone ever purchased from Gorilla Scientific? > www.GorillaScientific.com I just got a flyer in the mail from them and > it seems like they have some really good prices. I think I can save a > lot on my slides if I purchase from them. Has anyone else on here > purchased anything from them? Thanks, Alex histosearch@gmail.com > Well it was not Alex who posted it was Matthew Semovoski who posted this > & he is the man who answers the phone when you call up Gorilla > Scientific. I as a sales person play fair & do not try to do any slick > sales adds on histonet & wanted to make you aware of this. (I really am > such a geek & LOVE TO LEARN from Histonet too!) So I really do want to > thank you for all of the hard work that you do & just wanted to make you > aware of this instance. Thank you so much & have a great weekend! > > > I don't want to turn this into a major discussion but would like to > remind everyone that we run Histonet using University of Texas resources > and they would not appreciate (or support) its use for commercial > advertising even if it is disguised in a misleading message. Thank you, > Linda M Histonet administrator > > > > Please consider the environment before printing this e-mail. > > This e-mail, facsimile, or letter and any files or attachments > transmitted with it contains information that is confidential and > privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the > intended recipient, further disclosures are prohibited without proper > authorization. If you are not the intended recipient, any disclosure, > copying, printing, or use of this information is strictly prohibited and > possibly a violation of federal or state law and regulations. If you > have received this information in error, please notify Children's > Medical Center Dallas > immediately at 214-456-4444 or via e-mail at privacy@childrens.com. > Children's Medical Center Dallas and its affiliates hereby claim all > applicable privileges related to this information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From histomike <@t> charter.net Mon Jul 13 15:34:22 2009 From: histomike <@t> charter.net (histomike@charter.net) Date: Mon Jul 13 15:34:29 2009 Subject: [Histonet] Re: Histonet Digest, Vol 68, Issue 13 In-Reply-To: Message-ID: <20090713163422.ULJ5H.1740611.root@mp07> I was under the impression that a Bachelor's Degree was required in order to receive certification as a HTL. According to the ASCP: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Mike Schlicht, M.B.A., HLT (ASCP) Message: 11 Date: Mon, 13 Jul 2009 12:12:02 -0400 From: "Feher, Stephen" Subject: [Histonet] What percent of HTL's do not have a BS degree? To: Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> Content-Type: text/plain; charset="US-ASCII" I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From mucram11 <@t> comcast.net Mon Jul 13 15:45:28 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Jul 13 15:45:32 2009 Subject: [Histonet] Re: Histonet Digest, Vol 68, Issue 13 In-Reply-To: <20090713163422.ULJ5H.1740611.root@mp07> Message-ID: <1806645744.63171247517928041.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> No, some of the people who have HTL certs were grandfathered in as they worked in the field for years and many were and are superviors today.? It is easy to forget if you were not one of the early group who had the experience and not the degree that some exceptions were made with every advance.? Since I have known many of these people for years I would trust some them with my tissue and a diagnosis before some with degrees and less experience.? No offense just my opinion.? We all need to work together and respect each others experience and not just letters behing a name for all cases. Today you do need a BS to sit for the HTL and an associates for the HT. Pam Marcum HT, BS, MS, ----- Original Message ----- From: histomike@charter.net To: histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu Sent: Monday, July 13, 2009 4:34:22 PM GMT -05:00 US/Canada Eastern Subject: [Histonet] Re: Histonet Digest, Vol 68, Issue 13 I was under the impression that a Bachelor's Degree was required in order to receive certification as a HTL. According to the ASCP: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Mike Schlicht, M.B.A., HLT (ASCP) Message: 11 Date: Mon, 13 Jul 2009 12:12:02 -0400 From: "Feher, Stephen" Subject: [Histonet] What percent of HTL's do not have a BS degree? To: Message-ID: ????????<73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> Content-Type: text/plain;????????charset="US-ASCII" ? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. ?Does anyone have any solid references to support my position. ? Thanks, ? Steve ? ? Stephen A. Feher, MS, SCT (ASCP) ? Pathology Supervisor ? Catholic Medical Center ? 100 McGregor Street ? Manchester, NH 03102 ? 603-663-6707 ? sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From disbrc <@t> shands.ufl.edu Mon Jul 13 16:19:42 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Mon Jul 13 16:20:17 2009 Subject: [Histonet] What percent of HTL do not have a BS? Message-ID: <4A5B6CAE.72AC.0059.0@shands.ufl.edu> Hi! The bigger question is are there better jobs available for HTL's with or without a BS. Also, I thought ASCP required the BS for the HTL certification. I, for one, have been working very hard to get my BS so I can upgrade my certification to HTL. Many of the people in the lab I'm in have their BS and have not bothered to upgrade to an HTL. The other think I'm seeing is that quite a few of the newer students already have their BS and are completing the NACCLS approved programs. Thank you, Carrie From jnocito <@t> satx.rr.com Mon Jul 13 16:31:03 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jul 13 16:31:16 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D0F51CE6@SJSNT-SCMAIL03.stjoe.org> References: <4A5B3951.F783.00DA.0@childrens.com> <97C02552ECB11346877D3E83CF833ABD13D0F51CE6@SJSNT-SCMAIL03.stjoe.org> Message-ID: <85ABA87DBD134E2980EBBAACD33A057C@JoePC> let's stop monkeying around OK? (couldn't resist) JTT ----- Original Message ----- From: "Maria Katleba" To: "LINDA MARGRAF" ; Sent: Monday, July 13, 2009 1:48 PM Subject: RE: [Histonet] misleading message on Histonet last Friday OMG... I do not like primates (especially gorillas)... So I probably won't be buying anything from them any time soon. Just kidding. Seriously, I probably would stay away from that kind of a marketing name unless you are appealing to primate research facilities. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Mon Jul 13 16:32:28 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Jul 13 16:32:38 2009 Subject: [Histonet] misleading message on Histonet last Friday In-Reply-To: <85ABA87DBD134E2980EBBAACD33A057C@JoePC> References: <4A5B3951.F783.00DA.0@childrens.com> <97C02552ECB11346877D3E83CF833ABD13D0F51CE6@SJSNT-SCMAIL03.stjoe.org> <85ABA87DBD134E2980EBBAACD33A057C@JoePC> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F51E3F@SJSNT-SCMAIL03.stjoe.org> OMG! Now I am laughing too hard!!! Maria -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Monday, July 13, 2009 2:31 PM To: Maria Katleba; LINDA MARGRAF; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] misleading message on Histonet last Friday let's stop monkeying around OK? (couldn't resist) JTT ----- Original Message ----- From: "Maria Katleba" To: "LINDA MARGRAF" ; Sent: Monday, July 13, 2009 1:48 PM Subject: RE: [Histonet] misleading message on Histonet last Friday OMG... I do not like primates (especially gorillas)... So I probably won't be buying anything from them any time soon. Just kidding. Seriously, I probably would stay away from that kind of a marketing name unless you are appealing to primate research facilities. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, July 13, 2009 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] misleading message on Histonet last Friday Dear Histonetters: I got the message below privately from one of the list members and (with their permission) wanted to pass it along to the membership..... Dear Linda: Matthew Semovoski [histosearch@gmail.com] posted this on Histonet this AM: Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purchase from them. Has anyone else on here purchased anything from them? Thanks, Alex histosearch@gmail.com Well it was not Alex who posted it was Matthew Semovoski who posted this & he is the man who answers the phone when you call up Gorilla Scientific. I as a sales person play fair & do not try to do any slick sales adds on histonet & wanted to make you aware of this. (I really am such a geek & LOVE TO LEARN from Histonet too!) So I really do want to thank you for all of the hard work that you do & just wanted to make you aware of this instance. Thank you so much & have a great weekend! I don't want to turn this into a major discussion but would like to remind everyone that we run Histonet using University of Texas resources and they would not appreciate (or support) its use for commercial advertising even if it is disguised in a misleading message. Thank you, Linda M Histonet administrator Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Maria.Katleba <@t> stjoe.org Mon Jul 13 16:46:36 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Jul 13 16:46:42 2009 Subject: [Histonet] FT HISTOTECH NEEDED ASAP- NAPA CA In-Reply-To: <85ABA87DBD134E2980EBBAACD33A057C@JoePC> References: <4A5B3951.F783.00DA.0@childrens.com> <97C02552ECB11346877D3E83CF833ABD13D0F51CE6@SJSNT-SCMAIL03.stjoe.org> <85ABA87DBD134E2980EBBAACD33A057C@JoePC> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F51E62@SJSNT-SCMAIL03.stjoe.org> Hi All, We are in need of a licensed (or can sit for the exam) Histotech with experience of course. Full Time 2-3am start time... a path assistant comes in at 5am to help you coverslip and hand out the work. Hours somewhat flexible. Good Benefits, vacation, etc Available immediately! Call or fax me your resume. Also apply online www.TheQueen.org Maria Katleba HT(ASCP) BS, MS QVMC Pathology Dept. Mgr Napa CA 94558 707-294-9229 cell 707-257-4076 lab 707-257-4133 fax Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From ddubs0001 <@t> msn.com Mon Jul 13 16:33:18 2009 From: ddubs0001 <@t> msn.com (ddubs0001@msn.com) Date: Mon Jul 13 17:07:20 2009 Subject: [Histonet] Vacation reply In-Reply-To: Message-ID:
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From lpwenk <@t> sbcglobal.net Mon Jul 13 18:55:25 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jul 13 18:55:39 2009 Subject: [Histonet] Re: Histonet Digest, Vol 68, Issue 13 In-Reply-To: <1806645744.63171247517928041.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <5DCD700FBD2F4A318BBD3E64F4D83E48@HPPav2> Just for a little clarification - for 3 years (6 exams) starting in August 1980, ASCP did allow people who were certified histologic technicians (HT) with a minimum of 8 years experience (4 year college degree = 8 years experience) to sit for the Histotechnologist (HTL) exam, regardless of the type of education (some college, degree, high school diploma, etc). If they passed the HTL exam, they were now HTL(ASCP). So there was no "grandfathering" in. They still had to pay for, take and pass the HTL exam, the same as everyone else with the BS degree and minimum of 1 year's experience. Peggy Wenk -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Monday, July 13, 2009 4:45 PM To: histomike@charter.net Cc: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 68, Issue 13 No, some of the people who have HTL certs were grandfathered in as they worked in the field for years and many were and are superviors today.? It is easy to forget if you were not one of the early group who had the experience and not the degree that some exceptions were made with every advance.? Since I have known many of these people for years I would trust some them with my tissue and a diagnosis before some with degrees and less experience.? No offense just my opinion.? We all need to work together and respect each others experience and not just letters behing a name for all cases. Today you do need a BS to sit for the HTL and an associates for the HT. Pam Marcum HT, BS, MS, ----- Original Message ----- From: histomike@charter.net To: histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu Sent: Monday, July 13, 2009 4:34:22 PM GMT -05:00 US/Canada Eastern Subject: [Histonet] Re: Histonet Digest, Vol 68, Issue 13 I was under the impression that a Bachelor's Degree was required in order to receive certification as a HTL. According to the ASCP: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Mike Schlicht, M.B.A., HLT (ASCP) Message: 11 Date: Mon, 13 Jul 2009 12:12:02 -0400 From: "Feher, Stephen" Subject: [Histonet] What percent of HTL's do not have a BS degree? To: Message-ID: ????????<73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> Content-Type: text/plain;????????charset="US-ASCII" ? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. ?Does anyone have any solid references to support my position. ? Thanks, ? Steve ? ? Stephen A. Feher, MS, SCT (ASCP) ? Pathology Supervisor ? Catholic Medical Center ? 100 McGregor Street ? Manchester, NH 03102 ? 603-663-6707 ? sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nefff <@t> staff.uni-marburg.de Tue Jul 14 07:17:40 2009 From: nefff <@t> staff.uni-marburg.de (Dr. Frauke Neff) Date: Tue Jul 14 07:17:53 2009 Subject: [Histonet] myelin basic protein Message-ID: <20090714141740.9k594htsg8cg0gcw@home.staff.uni-marburg.de> Hi everbody, I'm currently looking for an antibody that stains myelin in mouse brains (no animals with EAE). Does anyone have experences with antibodies against MBP, MAG or other myelin proteins in mouse brains and can give me a suggestion, which one is the best to order for paraffin embedded tissue? Thanks a lot!!!! Frauke From dunatrsd <@t> sbcglobal.net Tue Jul 14 08:53:23 2009 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Tue Jul 14 08:53:26 2009 Subject: [Histonet] Can I get some help with Survivin ab. In-Reply-To: References: Message-ID: <418598.10512.qm@web83905.mail.sp1.yahoo.com> Hallo Everyone, For those of you who have used this antibody on FFPE human breast tissue, can you provide me with a protocol and antibody information? I have tried antibodies from Cell Signaling, Spring and two from Biocare, however I am not getting the results that are the same as in the publications. Any help/response would be greatly appreciated. thanks you and have a very good day. ? Dusko Trajkovic DSRD Scientist Pfizer Inc La Jolla 858-638-6202 From arvidsonkristen <@t> yahoo.com Tue Jul 14 09:13:20 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Jul 14 09:13:24 2009 Subject: [Histonet] Special stainers Message-ID: <555330.79780.qm@web65716.mail.ac4.yahoo.com> What stainer is everyone liking the best? From deepali.dhar <@t> yale.edu Tue Jul 14 09:25:43 2009 From: deepali.dhar <@t> yale.edu (Deepali Dhar) Date: Tue Jul 14 09:25:47 2009 Subject: [Histonet] human dendritic cell staining Message-ID: <2066dfa20907140725h118e5d8rc1bfba5b62a36d5e@mail.gmail.com> Hello! I am trying to stain dendritic cells (DCs) - from the immune system (not the brain) - in formalin/paraffin sections. I've heard that fascin is a commonly used marker but I don't think it's very specific - I see some endothelial cell staining too. Looking online, I've also found other possible markers, namely CD11c, CD68, and 33D1. Having never done this staining before, 1. Which marker for DC is routinely use in Immunohistochem (IHC)? 2. Are there other possible markers that are specific to DCs for IHC? Do these markers stain only DCs or also monocytes, macrophages, etc? 3. Also, if you have done staining for any of these markers, could you suggest antibodies and/or protocols? Thank you! Deepali Dhar Yale School of Medicine 2012 From koellingr <@t> comcast.net Tue Jul 14 09:34:48 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Jul 14 09:34:55 2009 Subject: [Histonet] TUNEL_FFPE_very_long OT_Delete_if_uninterested In-Reply-To: Message-ID: <2030442846.825261247582088332.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Hi Jerry, I would never try to persuade anyone.? I'm no smarter than the next lab worker trying to make sense of this and science biology in general. But this is how I see TUNEL and FFPE and after all the years I'm happy with things. I have not noticed false positives at all when looking at TUNEL FFPE versus frozen or whole cells and even when adapting the animal model to other verifying procedures (annexinV in flow) or caspace-3 when applicable.??Formalin can cause strand breaks, that is true?but these are not amplifiable breaks.? For TdT to work, the break must be of particular kinds.? But that discussion is far beyond the realm of possibility in HistoNet.? Up at UW, if you haven't done so, there is a great Cell and Molecular Biology Grad level course that is required in grad school and I'd recommend it for anyone.? It is phenomenal to help in understanding molecular mechanisms. When I was down the?hallway in grad school in Biological Structure, we had a PhD/MD student in adjacent lab who was working on apoptosis and macrophage scavenging in the developing limb buds (the web between "fingers") for a thesis.? Did TUNEL on FFPE and it was exquisite, beautiful and accepted as a (small part) of thesis.? Thousands of peer-reviewed articles in prestigous journals use TUNEL on FFPE.? Indeed, a review of specific articles where they are are trying to measure precisely strand breaks of DNA in UVA skin or germ-cell or other models, every one I see, the specimen is then placed into formalin or such for processing.? Why would they try to get a precise measurement of DNA strand breaks in their experimental model only to throw specimens into formalin if that is going to cause ubiquitous strand breaks and flood the assay?? All DNA strand breaks are not the same and the kind caused by formalin are simply not amplifiable by TdT. If they were, you could simply place a piece of normal, healthy mouse liver or some other tissue with non-apoptosing cells in formalin, process and every nuclei in liver, with broken strands from formalin would turn positive.? I've, never, ever seen such a thing in a well set up TUNEL assay.? And then if you do something to cause apoptosis, those nuclei are positive while others?remain unaffected by formalin.? Since I worked in thymus a lot,I had a control block of 4 thymii, one no treatment and the other 3 with varying time treatments of hydrocortisone to induce steroidal T-cell apoptosis (widely accepted model).? The differences where?easily recognizable?in apoptosis and when run with other tissues, non-apoptosing cells were still clean as anything. (1) strand breaks by formalin not amplifiable in TUNEL, otherwise every nuclei formalin fixed would be pos.? Just not so. (2) microtomy could not possibly cause strandbreaks that are amplifiable. Cutting is working on a micron scale.? Pieces of DNA are at nanometer or Angstrom scale.? If it did, again, every nuclei would be possitive that the blade "sliced through",? And then why would frozen TUNEL be ok if a blade is slicing through DNA in those sections.? So I disagree that "every one of them (nuclei in section) is detectable by TUNEL".? Again as before a common piece of healthy mouse liver cut at 4 microns would show nuclei all over positive.? That just doesn't happen. What I've seen and done, with very specifically controlled experiments, the vast amount? of peer-reviewed literature and thinking through the processes at a molecular level, I just can't come to the conclusion that TUNEL on FFPE is a failed assay that cannot work.? No assay is perfect.? PCR has primer-dimer and other problems to deal with.? IHC has false pos and false neg to worry about.? Flow has Fc receptor problems to deal with.? ISH has stringency issues to create false pos and false neg.? I think there is beyond overwheming evidence that TUNEL on FFPE is an essential (if never perfect) tool in molecular science for apoptosis but as with?every assay?you have to be aware of limitations and problems.? I just don't believe at all that amplifiable formalin strand breaks and amplifiable microtome strand beaks are any problem at all and should not be a reason to turn from TUNEL on FFPE. But again, that is just my opinion that is no more valid than others who might differ. Ray Raymond Koelling PhenoPath Labs Seattle, WA? 98155 rocedure called microtomy. When a microtome bvlade passes through the nucleus of a cell it breaks a lot of DNA strands. And every one of them is detectable by TUNEL. I've heard of people getting rerasonable results with whole cells and frozen tissues, by for FFPE tissue, my current philosophy is: It is an assay that CANNOT work, even in principle. 'Course, I've been wrong about other things, I'm open to persuasion. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Lauren found her dream laptop. Find the PC that?s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Jul 14 09:42:12 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jul 14 09:42:16 2009 Subject: [Histonet] Special stainers In-Reply-To: <555330.79780.qm@web65716.mail.ac4.yahoo.com> Message-ID: The Artisan (DAKO) is the best. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 14, 2009 9:13 AM To: histonet Subject: [Histonet] Special stainers What stainer is everyone liking the best? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Tue Jul 14 09:57:14 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Tue Jul 14 09:57:37 2009 Subject: [Histonet] Sakura DRS Stainer Message-ID: <8CBD2AA820B20AD-12B8-9CC@webmail-mh10.sysops.aol.com> Is there anyone using the Sakura DRS Stainer for H&E Staining?? I am presently trying to validate an H&E stain using the following reagents?:? ?Hematoxylin? - Cardinal Health, 7211 Clarifier - Cardinal Health Bluing reagent - Cardinal Health Eosin Y - Cardinal Health I am decerating the slides for 5 minutes each in xylene (2 changes), 10 minutes in hematoxylin, 30 seconds in clarifier and bluing, and 1 minute in eosin....obviously?there are alochol and water exchanges in between.? (This protocol works perfect on a Leica XL stainer): The hematoxylin staining is very light.? I will continue to play with the hematoxylin and clarifier times, but if someone has something that works, I would appreciate it if you could share it with me! Thank you, Ann From annigyg <@t> gmail.com Tue Jul 14 09:59:26 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Jul 14 09:59:31 2009 Subject: [Histonet] Special stainers In-Reply-To: <555330.79780.qm@web65716.mail.ac4.yahoo.com> References: <555330.79780.qm@web65716.mail.ac4.yahoo.com> Message-ID: Artisan does it for me - good stains, good backup, few if any delivery delays, application specialists 'on tap' via email direct fromDako Denmark - AND the machine does a MEAN Warthin Starry!!! Annie in Arabia 2009/7/14 kristen arvidson > What stainer is everyone liking the best? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jmjohnson34 <@t> hotmail.com Tue Jul 14 10:00:25 2009 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Tue Jul 14 10:00:30 2009 Subject: [Histonet] Eosin in Alcohol Message-ID: A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: "The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue." _________________________________________________________________ Lauren found her dream laptop. Find the PC that?s right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290 From joseph-galbraith <@t> uiowa.edu Tue Jul 14 10:10:53 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Tue Jul 14 10:10:58 2009 Subject: [Histonet] Special stainers In-Reply-To: References: <555330.79780.qm@web65716.mail.ac4.yahoo.com> Message-ID: We also use and like the Artisan. Joe Galbraith University of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, July 14, 2009 9:42 AM To: kristen arvidson; histonet Subject: RE: [Histonet] Special stainers The Artisan (DAKO) is the best. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 14, 2009 9:13 AM To: histonet Subject: [Histonet] Special stainers What stainer is everyone liking the best? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Tue Jul 14 10:37:03 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jul 14 10:37:07 2009 Subject: [Histonet] Combining Cytology and Histology Message-ID: <53FC421CC200C5429929EDE6C3676F302E7DEC@msgebe34> Hello, Are there any sites that are using CoPath with one number wheel for accessioning Cytology and Histology cases together? Right now, we have two separate departments with Cytology ordering under "C09-" and Histology ordering under "S09-" and we have have totally separate specimen classes. We are wondering if it's possible to use a single number wheel, using a combined number such as "P09-" (for Pathology), but still have each department order their own tests. This way, for example, when Cytology enters a FNA specimen in the morning and we (Histology) get a biopsy on the same patient a few hours later, when we enter the patient we are notified by the CoPath system that specimens were already assigned to that patient and we can use the same accession number. When the case is complete, everything is in one tray and can be signed out all together. One of our docs wants to have everything from Histology and Cytology on one report for the patient. Is this possible? Can Cytology still result the case if we have one accession number for everything? Any help or ideas on this issue would be greatly appreciated. Thanks much! Karen Bauer Karen L. Bauer HT(ASCP), BS Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu From awatanabe <@t> tgen.org Tue Jul 14 12:16:32 2009 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Tue Jul 14 12:16:38 2009 Subject: [Histonet] Re: survivin antibody In-Reply-To: <20090714170314.9A815A7EC6@mr2.tgen.org> Message-ID: The survivin antibody that I have used and gotten good results with was Cell Signaling #2803 at concentrations between 1:30-1:50, using an autostainer with pretty standard protocols. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From akemiat3377 <@t> yahoo.com Tue Jul 14 12:50:54 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Jul 14 12:50:57 2009 Subject: [Histonet] Paraffin for Processing and embedding Message-ID: <358094.3055.qm@web31306.mail.mud.yahoo.com> Hi All, I am thinking of switching the paraffin we are using for our processor and for embedding.? We are using the Fisherbrand Paraplast Plus which contains DMSO for both the processor and for embedding. I used to use Suripath's paraplast and loved it.? I would like to know what everyone in histoland is using. Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 E-Mail: aallison-tacha@apmglab.com From David.Malott <@t> LHSC.ON.CA Tue Jul 14 13:37:34 2009 From: David.Malott <@t> LHSC.ON.CA (David Malott) Date: Tue Jul 14 13:37:46 2009 Subject: [Histonet] Film for electron microscopy Message-ID: <4A5C982E.4C28.0002.0@lhsc.on.ca> We have recently used MACO ORT25c film in unperforated 35mm format in transmission electron microscopy. It is touted as a replacement for Kodak Technical Pan Film. After dessicating the film, it demonstrates a separation of the emulsion from the film base primarily at the cut ends of film strips but also randomly along the length. This occurs before and during processing. We are interested to learn of any reports on this problem and any insight into causes and solutions. -------------------------------------------------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From igor.deyneko <@t> gmail.com Tue Jul 14 14:03:47 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Jul 14 14:03:51 2009 Subject: [Histonet] Human VIMENTIN and SMA IHC Message-ID: <35e16a770907141203h14ccc18bt2e3123d11c478182@mail.gmail.com> Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From tjasper <@t> copc.net Tue Jul 14 14:15:38 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Jul 14 14:15:44 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> Message-ID: <90354A475B420441B2A0396E5008D4965E3061@copc-sbs.COPC.local> Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBranton <@t> sarapath.com Tue Jul 14 14:22:18 2009 From: BBranton <@t> sarapath.com (Brian Branton) Date: Tue Jul 14 14:24:34 2009 Subject: [Histonet] Ventana Benchmark XT for sale Message-ID: Hello HistoNetters We are selling our Ventana Benchmark XT IHC stainer. If anyone is interested, please see the ad on our web site. http://www.sarapath.com/equipment4sale/ I would be happy to answer any questions, please feel free to email or contact me directly. Thank You Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology (941) 362-8963 (941) 362-8964 Fax From JWeems <@t> sjha.org Tue Jul 14 14:37:10 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jul 14 14:37:01 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <90354A475B420441B2A0396E5008D4965E3061@copc-sbs.COPC.local> References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> <90354A475B420441B2A0396E5008D4965E3061@copc-sbs.COPC.local> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA56679E4@ITSSSXM01V6.one.ads.che.org> Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From LSebree <@t> uwhealth.org Tue Jul 14 15:43:53 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 14 15:43:59 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <90354A475B420441B2A0396E5008D4965E3061@copc-sbs.COPC.local> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5890357060C@UWHC-MAIL01.uwhis.hosp.wisc.edu> Tom, I missed being grandfathered in by a matter of months and am about 10 years away from retirement. I personally know of several HTLs that don't have Bachelor degrees that are around my same age. So in my part of the Universe, non-degreed HTLs are slightly more prevalent than a needle in a haystack but not much. Been at it going on 35 years, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 2:16 PM To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaustin1967 <@t> gmail.com Tue Jul 14 15:50:27 2009 From: jaustin1967 <@t> gmail.com (Michael Bradley) Date: Tue Jul 14 15:50:32 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA56679E4@ITSSSXM01V6.one.ads.che.org> References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> <90354A475B420441B2A0396E5008D4965E3061@copc-sbs.COPC.local> <5D64396A0D4A5346BEBC759022AAEAA56679E4@ITSSSXM01V6.one.ads.che.org> Message-ID: HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo - > time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas > Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. I would say > that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or close > to it. First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. I've been doing histology for ~25 years. I've met > people from all over the country and various parts of the world. Truth > is there isn't an abundance of HTLs out there. Unlike the Medical Lab > world, with the basic differences between MTs and MLTs, anatomic path > does not exactly mirror that with the HTL and HT. It's true the MT and > HTL both require a Bachelor's, but responsibilities in most labs, etc., > generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with an > HTL without a Bachelor's, I wouldn't waste time trying to justify it. I > guess the bottom line is if you want an HTL, that person will almost > assuredly have a Bachelor's. If you want to hire someone without a > Bachelor's that is certified (HT) you'll have better luck. I think > having an HTL is a great thing. I honestly have never pursued it > (though eligible) as the circumstances of my career would not have > rewarded me for doing so. As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for better > pay, perks or responsibility. Again, no slam to HTLs just the way > things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. HTs have been doing a great deal of very good work for > years in this field. And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. I am > contending that requiring the candidate to have a Bachelor's degree will > eliminate a substantial number of very qualified people. Does anyone > have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Tue Jul 14 15:54:11 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 14 15:54:15 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5890357060E@UWHC-MAIL01.uwhis.hosp.wisc.edu> MB, I think your prediction, sadly, is true and that it is already happening. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Tuesday, July 14, 2009 3:50 PM To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo > - time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Thomas Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. I would > say that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or > close to it. First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. I've been doing histology for ~25 years. I've met > people from all over the country and various parts of the world. > Truth is there isn't an abundance of HTLs out there. Unlike the > Medical Lab world, with the basic differences between MTs and MLTs, > anatomic path does not exactly mirror that with the HTL and HT. It's > true the MT and HTL both require a Bachelor's, but responsibilities in > most labs, etc., generally do not hinge on someone being an HT vs. an > HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with > an HTL without a Bachelor's, I wouldn't waste time trying to justify > it. I guess the bottom line is if you want an HTL, that person will > almost assuredly have a Bachelor's. If you want to hire someone > without a Bachelor's that is certified (HT) you'll have better luck. > I think having an HTL is a great thing. I honestly have never pursued > it (though eligible) as the circumstances of my career would not have > rewarded me for doing so. As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for > better pay, perks or responsibility. Again, no slam to HTLs just the > way things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. HTs have been doing a great deal of very good work for > years in this field. And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. I am > contending that requiring the candidate to have a Bachelor's degree > will eliminate a substantial number of very qualified people. Does > anyone have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Jul 14 15:53:35 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Jul 14 15:55:04 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA56679E4@ITSSSXM01V6.one.ads.che.org> References: <73A7ED895EE0C24D9267ED814911DF190FDB1C38@exchange.cmc-nh.org> <90354A475B420441B2A0396E5008D4965E3061@copc-sbs.COPC.local> <5D64396A0D4A5346BEBC759022AAEAA56679E4@ITSSSXM01V6.one.ads.che.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE21AB10F1DF3@MERCERMAIL.MercerU.local> I am with you Joyce, still going strong for 47 years and with no plans to retire, actually working my day job and a part time. I may not make it to 100 like you, but they will roll me out feet first. There are more of us around than people know. Scary huh? Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, July 14, 2009 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As a matter of fact some employers may look at it as an over-qualification, or at least no justification for better pay, perks or responsibility. Again, no slam to HTLs just the way things are, at least in my experience. If you want to hire people without a Bachelor's I would definitely pursue HTs. HTs have been doing a great deal of very good work for years in this field. And it sounds like you're viewing the Bachelor's thing as limiting factor more than the HTL itself. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, July 13, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What percent of HTL's do not have a BS degree? I'm trying to find some solid statistics to justify being able to hire HTL (ASCP) candidates who do not have a Bachelor's degree. I am contending that requiring the candidate to have a Bachelor's degree will eliminate a substantial number of very qualified people. Does anyone have any solid references to support my position. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Tue Jul 14 16:14:54 2009 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue Jul 14 16:14:56 2009 Subject: [Histonet] Eosin in Alcohol In-Reply-To: References: Message-ID: "polycyclic aromatic flourescent compounds that in high concentrations" ???? I wouldn't think the 3 mls of eosin dropped in the last 95% alcohol could be considered "high concentration" but that's what keeps Histonet entertaining; I learn something new every day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Tuesday, July 14, 2009 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin in Alcohol A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: "The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue." _________________________________________________________________ Lauren found her dream laptop. Find the PC that's right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290__________ _____________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Tue Jul 14 16:18:00 2009 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Tue Jul 14 16:18:07 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: Message-ID: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic.?I have been in the histology field for 20 years and don't consider it a profession or a career, just?a job. Ron Martin, BS, HTL (ASCP) ----- Original Message ----- From: "Michael Bradley" To: "Joyce Weems" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. ?I am an HTL with a Bachelors Degree. ?I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. ?I was sadly mistaken. ?After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. ?I spent many nights and weekends studying and doing my stains for the test. ?I am proud of my accomplishments. ?It is a shame that our industry does not reconize the difference between HT and HTL. ?A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. ?The histology world doesn't look for well qualified workers they look for cheap labor. ?I have heard more than one pathologist state that "a monkey can do our job." ?I have also worked in a lab where they would hire someone with a GED to cut slides. ?A career in histology is for the most part a dead end and there is no future. ?As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo - > time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > ?[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas > Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. ?I would say > that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. ?Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or close > to it. ?First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. ?I've been doing histology for ~25 years. ?I've met > people from all over the country and various parts of the world. ?Truth > is there isn't an abundance of HTLs out there. ?Unlike the Medical Lab > world, with the basic differences between MTs and MLTs, anatomic path > does not exactly mirror that with the HTL and HT. ?It's true the MT and > HTL both require a Bachelor's, but responsibilities in most labs, etc., > generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with an > HTL without a Bachelor's, I wouldn't waste time trying to justify it. ?I > guess the bottom line is if you want an HTL, that person will almost > assuredly have a Bachelor's. ?If you want to hire someone without a > Bachelor's that is certified (HT) you'll have better luck. ?I think > having an HTL is a great thing. ?I honestly have never pursued it > (though eligible) as the circumstances of my career would not have > rewarded me for doing so. ?As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for better > pay, perks or responsibility. ?Again, no slam to HTLs just the way > things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. ?HTs have been doing a great deal of very good work for > years in this field. ?And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am > contending that requiring the candidate to have a Bachelor's degree will > eliminate a substantial number of very qualified people. ?Does anyone > have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. ?Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jul 14 16:37:13 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jul 14 16:37:27 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: I wouldn't say that histology is a dead end job, I mean, I jumped to a PA and I know that I couldn't have passed that exam without my histology experience. The idea of adding an HTL category was initially a good one. Trying to bring us up to the MT and CT levels, but honestly, I think it fell short. Even after I received my BS, people asked me if I was going for my HTL. I told them no because I saw no benefit from it. At that time, I felt that if I couldn't get hired as a supervisor with a BS and 20 years experience, with 12 as a supervisor, I wasn't going to get the job any way. I'm not degrading HTLs by a long shot. I'm just saying for me, it wasn't worth it. After I retired from the Air Force, I was directly hired as a supervisor without the HTL. Just my three cents (inflation you know) JTT ----- Original Message ----- From: To: "Michael Bradley" Cc: Sent: Tuesday, July 14, 2009 4:18 PM Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) ----- Original Message ----- From: "Michael Bradley" To: "Joyce Weems" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo - > time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas > Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. I would say > that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or close > to it. First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. I've been doing histology for ~25 years. I've met > people from all over the country and various parts of the world. Truth > is there isn't an abundance of HTLs out there. Unlike the Medical Lab > world, with the basic differences between MTs and MLTs, anatomic path > does not exactly mirror that with the HTL and HT. It's true the MT and > HTL both require a Bachelor's, but responsibilities in most labs, etc., > generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with an > HTL without a Bachelor's, I wouldn't waste time trying to justify it. I > guess the bottom line is if you want an HTL, that person will almost > assuredly have a Bachelor's. If you want to hire someone without a > Bachelor's that is certified (HT) you'll have better luck. I think > having an HTL is a great thing. I honestly have never pursued it > (though eligible) as the circumstances of my career would not have > rewarded me for doing so. As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for better > pay, perks or responsibility. Again, no slam to HTLs just the way > things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. HTs have been doing a great deal of very good work for > years in this field. And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. I am > contending that requiring the candidate to have a Bachelor's degree will > eliminate a substantial number of very qualified people. Does anyone > have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Tue Jul 14 16:45:45 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Jul 14 16:45:54 2009 Subject: [Histonet] RE: Combining Cytology and Histology In-Reply-To: <53FC421CC200C5429929EDE6C3676F302E7DEC@msgebe34> References: <53FC421CC200C5429929EDE6C3676F302E7DEC@msgebe34> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F52422@SJSNT-SCMAIL03.stjoe.org> We are NOT using Copath.... however... I think the only time you have to separate Cytology from Histology is when you are dealing with GYNs. Otherwise, you can and should use the S09 (or what ever you give Pathology Surgicals). Why would anyone want to have two separate reports on one specimen? Your pathologist is right on! MK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, July 14, 2009 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Combining Cytology and Histology Hello, Are there any sites that are using CoPath with one number wheel for accessioning Cytology and Histology cases together? Right now, we have two separate departments with Cytology ordering under "C09-" and Histology ordering under "S09-" and we have have totally separate specimen classes. We are wondering if it's possible to use a single number wheel, using a combined number such as "P09-" (for Pathology), but still have each department order their own tests. This way, for example, when Cytology enters a FNA specimen in the morning and we (Histology) get a biopsy on the same patient a few hours later, when we enter the patient we are notified by the CoPath system that specimens were already assigned to that patient and we can use the same accession number. When the case is complete, everything is in one tray and can be signed out all together. One of our docs wants to have everything from Histology and Cytology on one report for the patient. Is this possible? Can Cytology still result the case if we have one accession number for everything? Any help or ideas on this issue would be greatly appreciated. Thanks much! Karen Bauer Karen L. Bauer HT(ASCP), BS Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Maria.Katleba <@t> stjoe.org Tue Jul 14 16:47:40 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Jul 14 16:48:12 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: References: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F52424@SJSNT-SCMAIL03.stjoe.org> Too bad someone doesn't do a 'survey' on this site where the question can be answered with real data... MK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, July 14, 2009 2:37 PM To: Pathrm35@comcast.net; Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? I wouldn't say that histology is a dead end job, I mean, I jumped to a PA and I know that I couldn't have passed that exam without my histology experience. The idea of adding an HTL category was initially a good one. Trying to bring us up to the MT and CT levels, but honestly, I think it fell short. Even after I received my BS, people asked me if I was going for my HTL. I told them no because I saw no benefit from it. At that time, I felt that if I couldn't get hired as a supervisor with a BS and 20 years experience, with 12 as a supervisor, I wasn't going to get the job any way. I'm not degrading HTLs by a long shot. I'm just saying for me, it wasn't worth it. After I retired from the Air Force, I was directly hired as a supervisor without the HTL. Just my three cents (inflation you know) JTT ----- Original Message ----- From: To: "Michael Bradley" Cc: Sent: Tuesday, July 14, 2009 4:18 PM Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. Ron Martin, BS, HTL (ASCP) ----- Original Message ----- From: "Michael Bradley" To: "Joyce Weems" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo - > time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas > Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. I would say > that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or close > to it. First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. I've been doing histology for ~25 years. I've met > people from all over the country and various parts of the world. Truth > is there isn't an abundance of HTLs out there. Unlike the Medical Lab > world, with the basic differences between MTs and MLTs, anatomic path > does not exactly mirror that with the HTL and HT. It's true the MT and > HTL both require a Bachelor's, but responsibilities in most labs, etc., > generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with an > HTL without a Bachelor's, I wouldn't waste time trying to justify it. I > guess the bottom line is if you want an HTL, that person will almost > assuredly have a Bachelor's. If you want to hire someone without a > Bachelor's that is certified (HT) you'll have better luck. I think > having an HTL is a great thing. I honestly have never pursued it > (though eligible) as the circumstances of my career would not have > rewarded me for doing so. As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for better > pay, perks or responsibility. Again, no slam to HTLs just the way > things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. HTs have been doing a great deal of very good work for > years in this field. And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. I am > contending that requiring the candidate to have a Bachelor's degree will > eliminate a substantial number of very qualified people. Does anyone > have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From ploykasek <@t> phenopath.com Tue Jul 14 16:57:37 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jul 14 16:57:45 2009 Subject: [Histonet] HTL/BS Message-ID: Thought I would throw my 2 cents out there, too. I received my HTL back in the early 80's (hate to think how old that makes me!), I already had a BS when I went to histotech school. I have found that it has made a difference in my career to have the HTL. When I have worked in hospitals, I was on the same pay scale as the med techs, the HT's were not. When I received my QIHC, some kind med techs lobbied for me to get the same specialty certification bonus that the med techs received. I feel fortunate to have mostly been treated by physicians and co-workers with respect, and do feel that my work is a profession (& I enjoy it). I am thankful to have the wonderful experiences, and I go home & have a glass of wine on the bad days! Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Nikki.Wahlberg <@t> bsci.com Tue Jul 14 18:02:48 2009 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Tue Jul 14 18:02:52 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E2473@MAPMAIL02.bsci.bossci.com> I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. Nikki -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic.?I have been in the histology field for 20 years and don't consider it a profession or a career, just?a job. Ron Martin, BS, HTL (ASCP) ----- Original Message ----- From: "Michael Bradley" To: "Joyce Weems" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. ?I am an HTL with a Bachelors Degree. ?I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. ?I was sadly mistaken. ?After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. ?I spent many nights and weekends studying and doing my stains for the test. ?I am proud of my accomplishments. ?It is a shame that our industry does not reconize the difference between HT and HTL. ?A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. ?The histology world doesn't look for well qualified workers they look for cheap labor. ?I have heard more than one pathologist state that "a monkey can do our job." ?I have also worked in a lab where they would hire someone with a GED to cut slides. ?A career in histology is for the most part a dead end and there is no future. ?As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo > - time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > ?[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Thomas Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. ?I would > say that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. ?Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or > close to it. ?First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. ?I've been doing histology for ~25 years. ?I've met > people from all over the country and various parts of the world. ? > Truth is there isn't an abundance of HTLs out there. ?Unlike the > Medical Lab world, with the basic differences between MTs and MLTs, > anatomic path does not exactly mirror that with the HTL and HT. ?It's > true the MT and HTL both require a Bachelor's, but responsibilities in > most labs, etc., generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with > an HTL without a Bachelor's, I wouldn't waste time trying to justify > it. ?I guess the bottom line is if you want an HTL, that person will > almost assuredly have a Bachelor's. ?If you want to hire someone > without a Bachelor's that is certified (HT) you'll have better luck. ? > I think having an HTL is a great thing. ?I honestly have never pursued > it (though eligible) as the circumstances of my career would not have > rewarded me for doing so. ?As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for > better pay, perks or responsibility. ?Again, no slam to HTLs just the > way things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. ?HTs have been doing a great deal of very good work for > years in this field. ?And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am > contending that requiring the candidate to have a Bachelor's degree > will eliminate a substantial number of very qualified people. ?Does > anyone have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. ?Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please reply to the sender that > you have received the message in error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Tue Jul 14 18:28:43 2009 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Tue Jul 14 18:28:48 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E2473@MAPMAIL02.bsci.bossci.com> Message-ID: <567598616.1515341247614123673.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Just my opinion Nikki. Sorry you don't agree but at least I respect your opinion. ----- Original Message ----- From: "Nikki Wahlberg" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 7:02:48 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? ? I would just like to add that in my ?opinion it is people who make statements like the one below that are holding our field back from being seen as a career. ?The hospitals as well as the doctors are also to blame. ?I am very proud to have a B.S. and A.S.S. degree and an HTL certification. ?I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. ?It makes me sad to hear people say that this is just a job not a career. ?I do not believe that anyone should be allowed to just come off the street and do our job. ?It up to us as a community to demand that institutions require certification and recognize our educations. ?I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. ?I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. Nikki -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Tuesday, July 14, 2009 4:18 PM To: Michael Bradley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Mike, I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic.?I have been in the histology field for 20 years and don't consider it a profession or a career, just?a job. Ron Martin, BS, HTL (ASCP) ----- Original Message ----- From: "Michael Bradley" To: "Joyce Weems" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. ?I am an HTL with a Bachelors Degree. ?I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. ?I was sadly mistaken. ?After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. ?I spent many nights and weekends studying and doing my stains for the test. ?I am proud of my accomplishments. ?It is a shame that our industry does not reconize the difference between HT and HTL. ?A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. ?The histology world doesn't look for well qualified workers they look for cheap labor. ?I have heard more than one pathologist state that "a monkey can do our job." ?I have also worked in a lab where they would hire someone with a GED to cut slides. ?A career in histology is for the most part a dead end and there is no future. ?As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo > - time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > ?[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Thomas Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. ?I would > say that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. ?Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or > close to it. ?First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. ?I've been doing histology for ~25 years. ?I've met > people from all over the country and various parts of the world. ? > Truth is there isn't an abundance of HTLs out there. ?Unlike the > Medical Lab world, with the basic differences between MTs and MLTs, > anatomic path does not exactly mirror that with the HTL and HT. ?It's > true the MT and HTL both require a Bachelor's, but responsibilities in > most labs, etc., generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with > an HTL without a Bachelor's, I wouldn't waste time trying to justify > it. ?I guess the bottom line is if you want an HTL, that person will > almost assuredly have a Bachelor's. ?If you want to hire someone > without a Bachelor's that is certified (HT) you'll have better luck. ? > I think having an HTL is a great thing. ?I honestly have never pursued > it (though eligible) as the circumstances of my career would not have > rewarded me for doing so. ?As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for > better pay, perks or responsibility. ?Again, no slam to HTLs just the > way things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. ?HTs have been doing a great deal of very good work for > years in this field. ?And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am > contending that requiring the candidate to have a Bachelor's degree > will eliminate a substantial number of very qualified people. ?Does > anyone have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. ?Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please reply to the sender that > you have received the message in error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Tue Jul 14 18:32:06 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Jul 14 18:32:09 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E2473@MAPMAIL02.bsci.bossci.com> References: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E2473@MAPMAIL02.bsci.bossci.com> Message-ID: <4A5D1576.7090301@umn.edu> To Ron Martin................histology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: > > I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. > > Nikki > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net > Sent: Tuesday, July 14, 2009 4:18 PM > To: Michael Bradley > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > > > Mike, > > > > I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. > > > > Ron Martin, BS, HTL (ASCP) > > > ----- Original Message ----- > From: "Michael Bradley" > To: "Joyce Weems" > Cc: histonet@lists.utsouthwestern.edu > Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > HI all > > I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. > I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. > Just my 2 cents. > > MB proud HTL > On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > >> Honey... You are a mere child! There are some of us that have been in >> the business for 40+ years. I missed the grandfather approach by 7 mo >> - time that I didn't work moving from place to place with my military >> ex-husband. >> >> But I did finally get the degree and do the exam. But we're still >> around. And I'll probably be working till I'm 100!!! J:>) >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Thomas Jasper >> Sent: Tuesday, July 14, 2009 15:16 >> To: Feher, Stephen >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? >> >> Hi Steve, >> >> I've got no statistics to offer you...just an observation. I would >> say that finding an HTL, without a Bachelor's degree is akin to the >> proverbial needle in a haystack. Anyone that obtained their HTL, >> if/when they could be grandfathered in, is likely to be retired or >> close to it. First of all, most folks that went the OJT route for >> certification were eligible to sit for the HT only (to my knowledge). >> I've never met anyone with an HTL that did not have a Bachelor's as a >> pre-requisite. I've been doing histology for ~25 years. I've met >> people from all over the country and various parts of the world. >> Truth is there isn't an abundance of HTLs out there. Unlike the >> Medical Lab world, with the basic differences between MTs and MLTs, >> anatomic path does not exactly mirror that with the HTL and HT. It's >> true the MT and HTL both require a Bachelor's, but responsibilities in >> most labs, etc., generally do not hinge on someone being an HT vs. an HTL. >> >> A person like myself is probably more common (Bachelor's and an HT). >> Unless you know of someone in particular; that you want to hire, with >> an HTL without a Bachelor's, I wouldn't waste time trying to justify >> it. I guess the bottom line is if you want an HTL, that person will >> almost assuredly have a Bachelor's. If you want to hire someone >> without a Bachelor's that is certified (HT) you'll have better luck. >> I think having an HTL is a great thing. I honestly have never pursued >> it (though eligible) as the circumstances of my career would not have >> rewarded me for doing so. As a matter of fact some employers may look >> at it as an over-qualification, or at least no justification for >> better pay, perks or responsibility. Again, no slam to HTLs just the >> way things are, at least in my experience. >> >> If you want to hire people without a Bachelor's I would definitely >> pursue HTs. HTs have been doing a great deal of very good work for >> years in this field. And it sounds like you're viewing the Bachelor's >> thing as limiting factor more than the HTL itself. >> >> Good luck, >> Tom Jasper >> >> Thomas Jasper HT (ASCP) BAS >> Histology Supervisor >> Central Oregon Regional Pathology Services Bend, Oregon 97701 >> 541/693-2677 >> tjasper@copc.net >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, >> Stephen >> Sent: Monday, July 13, 2009 9:12 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] What percent of HTL's do not have a BS degree? >> >> I'm trying to find some solid statistics to justify being able to hire >> HTL (ASCP) candidates who do not have a Bachelor's degree. I am >> contending that requiring the candidate to have a Bachelor's degree >> will eliminate a substantial number of very qualified people. Does >> anyone have any solid references to support my position. >> >> Thanks, >> >> Steve >> >> >> Stephen A. Feher, MS, SCT (ASCP) >> >> Pathology Supervisor >> >> Catholic Medical Center >> >> 100 McGregor Street >> >> Manchester, NH 03102 >> >> 603-663-6707 >> >> sfeher@cmc-nh.org >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This email, including any attachments is the property of Catholic >> Health East and is intended for the sole use of the intended >> recipient(s). >> It may contain information that is privileged and confidential. Any >> unauthorized review, use, disclosure, or distribution is prohibited. >> If you are not the intended recipient, please reply to the sender that >> you have received the message in error, then delete this message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ynwang <@t> u.washington.edu Tue Jul 14 18:32:03 2009 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue Jul 14 18:32:28 2009 Subject: [Histonet] formalin substitutes - tissue structure Message-ID: <33b7cf2b0907141632x7a16321bhe6d8a67287fd3bfc@mail.gmail.com> Dear Histonetters, I have a question about alternatives to formalin fixation and fine changes in tissue structure. We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company. >From the Histonet archives I see that sometimes the use of formalin alternatives can affect immuno staining, but does anyone know how it would affect fine structure of tissue. My thoughts were that there may be a slight difference in 'shrinkage' that occurs given the main ingredient is ethanol on some of the alternatives, so fat fixed in one of these alternatives would give an erroneously smaller adipose cell size. Any insight would be greatly appreciated. Thank you Yak-Nam Wang University of Washington From Pathrm35 <@t> comcast.net Tue Jul 14 18:40:15 2009 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Tue Jul 14 18:40:20 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <4A5D1576.7090301@umn.edu> Message-ID: <1583115834.1518811247614815366.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Colleen, One of my points of frustration is that we have never been on the same level as MT's and MLT's. I feel that part of this is because of our level of education and requirements. After seeing little changes over the years it is frustrating. I'm sure I'm not the only one who feels this way. Yes, I would like to find another area to utilize my education and background. My apologies if I offended anyone as that was not my intention. All opinions should be respected. Ron ----- Original Message ----- From: "Colleen Forster" To: "Nikki Wahlberg" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 7:32:06 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? To Ron Martin................histology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: > ? > I would just like to add that in my ?opinion it is people who make statements like the one below that are holding our field back from being seen as a career. ?The hospitals as well as the doctors are also to blame. ?I am very proud to have a B.S. and A.S.S. degree and an HTL certification. ?I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. ?It makes me sad to hear people say that this is just a job not a career. ?I do not believe that anyone should be allowed to just come off the street and do our job. ?It up to us as a community to demand that institutions require certification and recognize our educations. ?I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. ?I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. > > Nikki > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net > Sent: Tuesday, July 14, 2009 4:18 PM > To: Michael Bradley > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > > > Mike, > > > > I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. > > > > Ron Martin, BS, HTL (ASCP) > > > ----- Original Message ----- > From: "Michael Bradley" > To: "Joyce Weems" > Cc: histonet@lists.utsouthwestern.edu > Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > HI all > > I am a rarity. ?I am an HTL with a Bachelors Degree. ?I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. ?I was sadly mistaken. ?After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. ?I spent many nights and weekends studying and doing my stains for the test. ?I am proud of my accomplishments. ?It is a shame that our industry does not reconize the difference between HT and HTL. ?A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. > I worked hard to no avail. ?The histology world doesn't look for well qualified workers they look for cheap labor. ?I have heard more than one pathologist state that "a monkey can do our job." ?I have also worked in a lab where they would hire someone with a GED to cut slides. ?A career in histology is for the most part a dead end and there is no future. ?As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. > Just my 2 cents. > > MB proud HTL > On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > ? >> Honey... You are a mere child! There are some of us that have been in >> the business for 40+ years. I missed the grandfather approach by 7 mo >> - time that I didn't work moving from place to place with my military >> ex-husband. >> >> But I did finally get the degree and do the exam. But we're still >> around. And I'll probably be working till I'm 100!!! J:>) >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> ?[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Thomas Jasper >> Sent: Tuesday, July 14, 2009 15:16 >> To: Feher, Stephen >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? >> >> Hi Steve, >> >> I've got no statistics to offer you...just an observation. ?I would >> say that finding an HTL, without a Bachelor's degree is akin to the >> proverbial needle in a haystack. ?Anyone that obtained their HTL, >> if/when they could be grandfathered in, is likely to be retired or >> close to it. ?First of all, most folks that went the OJT route for >> certification were eligible to sit for the HT only (to my knowledge). >> I've never met anyone with an HTL that did not have a Bachelor's as a >> pre-requisite. ?I've been doing histology for ~25 years. ?I've met >> people from all over the country and various parts of the world. ? >> Truth is there isn't an abundance of HTLs out there. ?Unlike the >> Medical Lab world, with the basic differences between MTs and MLTs, >> anatomic path does not exactly mirror that with the HTL and HT. ?It's >> true the MT and HTL both require a Bachelor's, but responsibilities in >> most labs, etc., generally do not hinge on someone being an HT vs. an HTL. >> >> A person like myself is probably more common (Bachelor's and an HT). >> Unless you know of someone in particular; that you want to hire, with >> an HTL without a Bachelor's, I wouldn't waste time trying to justify >> it. ?I guess the bottom line is if you want an HTL, that person will >> almost assuredly have a Bachelor's. ?If you want to hire someone >> without a Bachelor's that is certified (HT) you'll have better luck. ? >> I think having an HTL is a great thing. ?I honestly have never pursued >> it (though eligible) as the circumstances of my career would not have >> rewarded me for doing so. ?As a matter of fact some employers may look >> at it as an over-qualification, or at least no justification for >> better pay, perks or responsibility. ?Again, no slam to HTLs just the >> way things are, at least in my experience. >> >> If you want to hire people without a Bachelor's I would definitely >> pursue HTs. ?HTs have been doing a great deal of very good work for >> years in this field. ?And it sounds like you're viewing the Bachelor's >> thing as limiting factor more than the HTL itself. >> >> Good luck, >> Tom Jasper >> >> Thomas Jasper HT (ASCP) BAS >> Histology Supervisor >> Central Oregon Regional Pathology Services Bend, Oregon 97701 >> 541/693-2677 >> tjasper@copc.net >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, >> Stephen >> Sent: Monday, July 13, 2009 9:12 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] What percent of HTL's do not have a BS degree? >> >> I'm trying to find some solid statistics to justify being able to hire >> HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am >> contending that requiring the candidate to have a Bachelor's degree >> will eliminate a substantial number of very qualified people. ?Does >> anyone have any solid references to support my position. >> >> Thanks, >> >> Steve >> >> >> Stephen A. Feher, MS, SCT (ASCP) >> >> Pathology Supervisor >> >> Catholic Medical Center >> >> 100 McGregor Street >> >> Manchester, NH 03102 >> >> 603-663-6707 >> >> sfeher@cmc-nh.org >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This email, including any attachments is the property of Catholic >> Health East and is intended for the sole use of the intended >> recipient(s). >> It may contain information that is privileged and confidential. ?Any >> unauthorized review, use, disclosure, or distribution is prohibited. >> If you are not the intended recipient, please reply to the sender that >> you have received the message in error, then delete this message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ? ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thecitan <@t> yahoo.com Tue Jul 14 19:28:56 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Tue Jul 14 19:28:17 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <1583115834.1518811247614815366.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <4A5D1576.7090301@umn.edu><1583115834.1518811247614815366.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <358520986-1247617691-cardhu_decombobulator_blackberry.rim.net-137161816-@bxe1123.bisx.prod.on.blackberry> Hey everyone, I'm actually a early 20s histotech without a certification and run my own routine histo lab. *waits for "young whiper snapper" remark* But I am in the process of completing my education. In all seriousness though I have to agree many out there are looking for cheap labor - so I take it upon myself to get my cert through a local program and look for jobs that are more management based and less bench work (or high paying contract work) Honestly the only true advancement I see in my career is management or consulting, but take that with a grain of salt - and throw in some fiber while your at it :P. I've only been in the field for a year. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Pathrm35@comcast.net Date: Tue, 14 Jul 2009 23:40:15 To: Colleen Forster Cc: Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? Colleen, One of my points of frustration is that we have never been on the same level as MT's and MLT's. I feel that part of this is because of our level of education and requirements. After seeing little changes over the years it is frustrating. I'm sure I'm not the only one who feels this way. Yes, I would like to find another area to utilize my education and background. My apologies if I offended anyone as that was not my intention. All opinions should be respected. Ron ----- Original Message ----- From: "Colleen Forster" To: "Nikki Wahlberg" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 14, 2009 7:32:06 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? To Ron Martin................histology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: > ? > I would just like to add that in my ?opinion it is people who make statements like the one below that are holding our field back from being seen as a career. ?The hospitals as well as the doctors are also to blame. ?I am very proud to have a B.S. and A.S.S. degree and an HTL certification. ?I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. ?It makes me sad to hear people say that this is just a job not a career. ?I do not believe that anyone should be allowed to just come off the street and do our job. ?It up to us as a community to demand that institutions require certification and recognize our educations. ?I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. ?I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. > > Nikki > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net > Sent: Tuesday, July 14, 2009 4:18 PM > To: Michael Bradley > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > > > Mike, > > > > I couldn't agree with you more. I'm in the same position as you. In the Boston, Mass. area people are taken right off the street and work for a year as a lab assistant then promoted to a tech in training. Most have a hs diploma, no ambition and expect good pay for bad work and poor work ethic. I have been in the histology field for 20 years and don't consider it a profession or a career, just a job. > > > > Ron Martin, BS, HTL (ASCP) > > > ----- Original Message ----- > From: "Michael Bradley" > To: "Joyce Weems" > Cc: histonet@lists.utsouthwestern.edu > Sent: Tuesday, July 14, 2009 4:50:27 PM GMT -05:00 US/Canada Eastern > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > HI all > > I am a rarity. ?I am an HTL with a Bachelors Degree. ?I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. ?I was sadly mistaken. ?After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. ?I spent many nights and weekends studying and doing my stains for the test. ?I am proud of my accomplishments. ?It is a shame that our industry does not reconize the difference between HT and HTL. ?A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. > I worked hard to no avail. ?The histology world doesn't look for well qualified workers they look for cheap labor. ?I have heard more than one pathologist state that "a monkey can do our job." ?I have also worked in a lab where they would hire someone with a GED to cut slides. ?A career in histology is for the most part a dead end and there is no future. ?As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. > Just my 2 cents. > > MB proud HTL > On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > ? >> Honey... You are a mere child! There are some of us that have been in >> the business for 40+ years. I missed the grandfather approach by 7 mo >> - time that I didn't work moving from place to place with my military >> ex-husband. >> >> But I did finally get the degree and do the exam. But we're still >> around. And I'll probably be working till I'm 100!!! J:>) >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> ?[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Thomas Jasper >> Sent: Tuesday, July 14, 2009 15:16 >> To: Feher, Stephen >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? >> >> Hi Steve, >> >> I've got no statistics to offer you...just an observation. ?I would >> say that finding an HTL, without a Bachelor's degree is akin to the >> proverbial needle in a haystack. ?Anyone that obtained their HTL, >> if/when they could be grandfathered in, is likely to be retired or >> close to it. ?First of all, most folks that went the OJT route for >> certification were eligible to sit for the HT only (to my knowledge). >> I've never met anyone with an HTL that did not have a Bachelor's as a >> pre-requisite. ?I've been doing histology for ~25 years. ?I've met >> people from all over the country and various parts of the world. ? >> Truth is there isn't an abundance of HTLs out there. ?Unlike the >> Medical Lab world, with the basic differences between MTs and MLTs, >> anatomic path does not exactly mirror that with the HTL and HT. ?It's >> true the MT and HTL both require a Bachelor's, but responsibilities in >> most labs, etc., generally do not hinge on someone being an HT vs. an HTL. >> >> A person like myself is probably more common (Bachelor's and an HT). >> Unless you know of someone in particular; that you want to hire, with >> an HTL without a Bachelor's, I wouldn't waste time trying to justify >> it. ?I guess the bottom line is if you want an HTL, that person will >> almost assuredly have a Bachelor's. ?If you want to hire someone >> without a Bachelor's that is certified (HT) you'll have better luck. ? >> I think having an HTL is a great thing. ?I honestly have never pursued >> it (though eligible) as the circumstances of my career would not have >> rewarded me for doing so. ?As a matter of fact some employers may look >> at it as an over-qualification, or at least no justification for >> better pay, perks or responsibility. ?Again, no slam to HTLs just the >> way things are, at least in my experience. >> >> If you want to hire people without a Bachelor's I would definitely >> pursue HTs. ?HTs have been doing a great deal of very good work for >> years in this field. ?And it sounds like you're viewing the Bachelor's >> thing as limiting factor more than the HTL itself. >> >> Good luck, >> Tom Jasper >> >> Thomas Jasper HT (ASCP) BAS >> Histology Supervisor >> Central Oregon Regional Pathology Services Bend, Oregon 97701 >> 541/693-2677 >> tjasper@copc.net >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, >> Stephen >> Sent: Monday, July 13, 2009 9:12 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] What percent of HTL's do not have a BS degree? >> >> I'm trying to find some solid statistics to justify being able to hire >> HTL (ASCP) candidates who do not have a Bachelor's degree. ?I am >> contending that requiring the candidate to have a Bachelor's degree >> will eliminate a substantial number of very qualified people. ?Does >> anyone have any solid references to support my position. >> >> Thanks, >> >> Steve >> >> >> Stephen A. Feher, MS, SCT (ASCP) >> >> Pathology Supervisor >> >> Catholic Medical Center >> >> 100 McGregor Street >> >> Manchester, NH 03102 >> >> 603-663-6707 >> >> sfeher@cmc-nh.org >> >> >>_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >>_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This email, including any attachments is the property of Catholic >> Health East and is intended for the sole use of the intended >> recipient(s). >> It may contain information that is privileged and confidential. ?Any >> unauthorized review, use, disclosure, or distribution is prohibited. >> If you are not the intended recipient, please reply to the sender that >> you have received the message in error, then delete this message. >> >> >>_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ? ? >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.PALMER <@t> svhm.org.au Tue Jul 14 21:33:40 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Tue Jul 14 21:34:15 2009 Subject: [Histonet] RE: human vimentin IHC Message-ID: Igor. A few years back I used Dako V9 mouse anti human vimentin to label human grafts in a mouse background. Tested it first on several mouse tissues and got no reactivity, compared to very strong reactivity in a variety of cell types in human tissue, and so am sure that it is human specific cf mouse. I used the Dako ARK to get around the mouse-on-mouse background issues and was happy with the staining obtained (although not quite as sensitive perhaps as a standard, LSAB method). I am actually about to try this again myself very soon. I used citrate AR and primary at 1:800 for my staining. Cheers, Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ------------------------------ Message: 4 Date: Tue, 14 Jul 2009 15:03:47 -0400 From: Igor Deyneko Subject: [Histonet] Human VIMENTIN and SMA IHC To: Histonet@lists.utsouthwestern.edu Message-ID: <35e16a770907141203h14ccc18bt2e3123d11c478182@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jshea121 <@t> roadrunner.com Tue Jul 14 22:12:53 2009 From: jshea121 <@t> roadrunner.com (Shea's) Date: Tue Jul 14 22:12:57 2009 Subject: [Histonet] HTL Message-ID: Michael, Ditto, very well stated. I too believe that our industry is under appreciated. Many new grads of today find a two year degree demeaning and wouldn't consider HT because of it. I don't understand how some professions like pharmacy & physical therapy gain respect and grow to create 5 yr, 6yr & 7yr programs. They are very well respected by the MDs and Hospital administration and have nice salaries to show for it. Why hasn't our field flourished? Jan, BS, HTL From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Jul 15 02:16:59 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 15 02:19:03 2009 Subject: [Histonet] HTL Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0720AB42@wahtntex2.waht.swest.nhs.uk> Do you want the blunt truth? There's a perception, even within the other disciplines in Diagnostic Labs, that BMS's in Histology (HistoTechs) are second rate Scientists. I know that's an inflammatory remark but I've battled with it for years. Pharmacists, Physiotherapists, Ots, Audiologists and Speech Language Therapists run Clinics treat Patients and are 'clinical'. The perception is that 'scientists' are not clinical and before we get appreciated for that we probably need to run Clinics ourselves but how do Histotechs/ BMS's achieve that? In the UK scientific staff are slowly doing that with Anticoagulant Clinics, with advanced dissection and the reporting of cervical smears after achieving the appropriate level of qualification. I'm hoping one day that the 'glass ceiling' will be taken off the Path Labs and that a scientist will, after obtaining his/ her degree, Masters (or PhD), like the Clinical Scientists, obtain the MRCPath and then clinically lead a discipline. Only when we step from behind the skirts of the Medics will the sun shine on us. Does that help? Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: 15 July 2009 04:13 To: jaustin1967@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL Michael, Ditto, very well stated. I too believe that our industry is under appreciated. Many new grads of today find a two year degree demeaning and wouldn't consider HT because of it. I don't understand how some professions like pharmacy & physical therapy gain respect and grow to create 5 yr, 6yr & 7yr programs. They are very well respected by the MDs and Hospital administration and have nice salaries to show for it. Why hasn't our field flourished? Jan, BS, HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Jul 15 02:26:13 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 15 02:31:09 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0720AB46@wahtntex2.waht.swest.nhs.uk> "The histology world doesn't look for well qualified workers they look for cheap labor (SIC). I have heard more than one pathologist state that "a monkey can do our job." " See my other post. The retort ought to be that a Histology BMS/ Histotech can do yours!! A honest Pathologist once told me that a good Histotech could report 80% of what he did, you needed some medical knowledge to maybe report the next 15% or so, Pathologists with a speciality probably reported the next 2% or 3% and it took an expert to deal with the top few percent. He taught me Pathology of the skin and I was good at it; I naturally then became a Cytologist as there's no way, without a MRCPath, that I could ever report skin biopsies. A Gynaecologist friend of mine once told that the Pathologist/ Histotech (BMS) relationship was perceived by many of his colleagues to be the last bastion of prostitution. I never figured out who was the pimp!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: 14 July 2009 21:50 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that "a monkey can do our job." I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > Honey... You are a mere child! There are some of us that have been in > the business for 40+ years. I missed the grandfather approach by 7 mo > - time that I didn't work moving from place to place with my military > ex-husband. > > But I did finally get the degree and do the exam. But we're still > around. And I'll probably be working till I'm 100!!! J:>) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Thomas Jasper > Sent: Tuesday, July 14, 2009 15:16 > To: Feher, Stephen > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? > > Hi Steve, > > I've got no statistics to offer you...just an observation. I would > say that finding an HTL, without a Bachelor's degree is akin to the > proverbial needle in a haystack. Anyone that obtained their HTL, > if/when they could be grandfathered in, is likely to be retired or > close to it. First of all, most folks that went the OJT route for > certification were eligible to sit for the HT only (to my knowledge). > I've never met anyone with an HTL that did not have a Bachelor's as a > pre-requisite. I've been doing histology for ~25 years. I've met > people from all over the country and various parts of the world. > Truth is there isn't an abundance of HTLs out there. Unlike the > Medical Lab world, with the basic differences between MTs and MLTs, > anatomic path does not exactly mirror that with the HTL and HT. It's > true the MT and HTL both require a Bachelor's, but responsibilities in > most labs, etc., generally do not hinge on someone being an HT vs. an HTL. > > A person like myself is probably more common (Bachelor's and an HT). > Unless you know of someone in particular; that you want to hire, with > an HTL without a Bachelor's, I wouldn't waste time trying to justify > it. I guess the bottom line is if you want an HTL, that person will > almost assuredly have a Bachelor's. If you want to hire someone > without a Bachelor's that is certified (HT) you'll have better luck. > I think having an HTL is a great thing. I honestly have never pursued > it (though eligible) as the circumstances of my career would not have > rewarded me for doing so. As a matter of fact some employers may look > at it as an over-qualification, or at least no justification for > better pay, perks or responsibility. Again, no slam to HTLs just the > way things are, at least in my experience. > > If you want to hire people without a Bachelor's I would definitely > pursue HTs. HTs have been doing a great deal of very good work for > years in this field. And it sounds like you're viewing the Bachelor's > thing as limiting factor more than the HTL itself. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, > Stephen > Sent: Monday, July 13, 2009 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What percent of HTL's do not have a BS degree? > > I'm trying to find some solid statistics to justify being able to hire > HTL (ASCP) candidates who do not have a Bachelor's degree. I am > contending that requiring the candidate to have a Bachelor's degree > will eliminate a substantial number of very qualified people. Does > anyone have any solid references to support my position. > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please reply to the sender that > you have received the message in error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Jul 15 02:43:50 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Jul 15 02:44:28 2009 Subject: [Histonet] Re: RE: human vimentin IHC Message-ID: <11D9615B89C10747B1C985966A63D7CA297722AE4C@KCL-MAIL04.kclad.ds.kcl.ac.uk> I agree with Jason: have a look in image gallery here http://www.immunoportal.com/ for an image of HES cells in mouse tissue, using V9 clone. No personal experience with Human specific SMA but plenty of images ( "ASMA") in IP gallery.... Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases King's College London Tel.020 7848 6810 From annigyg <@t> gmail.com Wed Jul 15 03:01:24 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Jul 15 03:01:30 2009 Subject: [Histonet] HTL In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0720AB42@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0720AB42@wahtntex2.waht.swest.nhs.uk> Message-ID: well said!! your statement: 'Only when we step from behind the skirts of the Medics will the sun shine on us' deserves dissection (pardon the pun) are we volutarily 'behind the medics" or are we conveniently 'kept' there by those same medics medics=pathologists (some exceptions) where i come from most of these 'medics' are running the (very lucrative) private labs and the techs are kept 'lean and hungry' - they are 'worker bees'' grateful for work and paid a pittance. i once voiced my desire to take unpaid leave in order to study further and was refused time off for this, on the basis that i would then cost more to employ!!! i have a 4 year diploma (now called a BTech degree) - i am licensed as a Medical Technologist with Cell Path Speciality. i am neither an HT or an HTL. i have 30 years experience and have been supervising/managing AP labs for over 15 years but because i dont have a degree i would most likely have a hard time finding employment in the USA or Canada - your loss guys. its not what you call it its how you apply what you know - having a degree does not make you a good tech. flame away!! AnnieinArabia (out of Africa) 2009/7/15 Kemlo Rogerson > Do you want the blunt truth? > > There's a perception, even within the other disciplines in Diagnostic > Labs, that BMS's in Histology (HistoTechs) are second rate Scientists. I > know that's an inflammatory remark but I've battled with it for years. > Pharmacists, Physiotherapists, Ots, Audiologists and Speech Language > Therapists run Clinics treat Patients and are 'clinical'. The perception > is that 'scientists' are not clinical and before we get appreciated for > that we probably need to run Clinics ourselves but how do Histotechs/ > BMS's achieve that? In the UK scientific staff are slowly doing that > with Anticoagulant Clinics, with advanced dissection and the reporting > of cervical smears after achieving the appropriate level of > qualification. > > I'm hoping one day that the 'glass ceiling' will be taken off the Path > Labs and that a scientist will, after obtaining his/ her degree, Masters > (or PhD), like the Clinical Scientists, obtain the MRCPath and then > clinically lead a discipline. Only when we step from behind the skirts > of the Medics will the sun shine on us. > > Does that help? > > > > > > > > > Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's > Sent: 15 July 2009 04:13 > To: jaustin1967@gmail.com > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HTL > > Michael, > Ditto, very well stated. I too believe that our industry is under > appreciated. Many new grads of today find a two year degree demeaning > and wouldn't consider HT because of it. I don't understand how some > professions like pharmacy & physical therapy gain respect and grow to > create 5 yr, 6yr & 7yr programs. They are very well respected by the MDs > and Hospital administration and have nice salaries to show for it. > > Why hasn't our field flourished? > Jan, BS, HTL > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From thecitan <@t> yahoo.com Wed Jul 15 03:55:32 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Wed Jul 15 03:54:49 2009 Subject: [Histonet] HTL In-Reply-To: References: <86ADE4EB583CE64799A9924684A0FBBF0720AB42@wahtntex2.waht.swest.nhs.uk> Message-ID: <211981232-1247648083-cardhu_decombobulator_blackberry.rim.net-1084216680-@bxe1123.bisx.prod.on.blackberry> Anne I think most techs I know are in the voluntary category you speak of. Most are happy being microtome monkeys and never exploring the other possibilities in the field. Nothing wrong with that if that's what you want to do. Although there are many things you can do with - like anne said- applying what you know. As far as pathologists keeping you back -i think its just like any other business. The boss will always look to keep more money and will pay his workers the lowest he can. That's when you take your experience elsewhere, or simply stay somewhere for a while to learn and beef up that resume. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Anne van Binsbergen Date: Wed, 15 Jul 2009 12:01:24 To: Kemlo Rogerson Cc: ; Shea's Subject: Re: [Histonet] HTL well said!! your statement: 'Only when we step from behind the skirts of the Medics will the sun shine on us' deserves dissection (pardon the pun) are we volutarily 'behind the medics" or are we conveniently 'kept' there by those same medics medics=pathologists (some exceptions) where i come from most of these 'medics' are running the (very lucrative) private labs and the techs are kept 'lean and hungry' - they are 'worker bees'' grateful for work and paid a pittance. i once voiced my desire to take unpaid leave in order to study further and was refused time off for this, on the basis that i would then cost more to employ!!! i have a 4 year diploma (now called a BTech degree) - i am licensed as a Medical Technologist with Cell Path Speciality. i am neither an HT or an HTL. i have 30 years experience and have been supervising/managing AP labs for over 15 years but because i dont have a degree i would most likely have a hard time finding employment in the USA or Canada - your loss guys. its not what you call it its how you apply what you know - having a degree does not make you a good tech. flame away!! AnnieinArabia (out of Africa) 2009/7/15 Kemlo Rogerson > Do you want the blunt truth? > > There's a perception, even within the other disciplines in Diagnostic > Labs, that BMS's in Histology (HistoTechs) are second rate Scientists. I > know that's an inflammatory remark but I've battled with it for years. > Pharmacists, Physiotherapists, Ots, Audiologists and Speech Language > Therapists run Clinics treat Patients and are 'clinical'. The perception > is that 'scientists' are not clinical and before we get appreciated for > that we probably need to run Clinics ourselves but how do Histotechs/ > BMS's achieve that? In the UK scientific staff are slowly doing that > with Anticoagulant Clinics, with advanced dissection and the reporting > of cervical smears after achieving the appropriate level of > qualification. > > I'm hoping one day that the 'glass ceiling' will be taken off the Path > Labs and that a scientist will, after obtaining his/ her degree, Masters > (or PhD), like the Clinical Scientists, obtain the MRCPath and then > clinically lead a discipline. Only when we step from behind the skirts > of the Medics will the sun shine on us. > > Does that help? > > > > > > > > > Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's > Sent: 15 July 2009 04:13 > To: jaustin1967@gmail.com > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HTL > > Michael, > Ditto, very well stated. I too believe that our industry is under > appreciated. Many new grads of today find a two year degree demeaning > and wouldn't consider HT because of it. I don't understand how some > professions like pharmacy & physical therapy gain respect and grow to > create 5 yr, 6yr & 7yr programs. They are very well respected by the MDs > and Hospital administration and have nice salaries to show for it. > > Why hasn't our field flourished? > Jan, BS, HTL > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Jul 15 04:32:35 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jul 15 04:32:38 2009 Subject: [Histonet] Re: formalin substitutes - tissue structure Message-ID: Yak-Nam Wang at the University of Washington (in the state of Washington USA) asks: >>We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company.<< Depends on whether you're doing science or not. Prefer is Anatech's glyoxal-based fixative, and they can probably offer you some guidance. Glyoxal is an aldehyde fixative. It may well be interchangeable for this purpose. FineFix is a secret formula by a company I never heard of, with a dysfunctional Web site. The fixative appears to be ethanol, which however is added by the user and is not in the formula. I couldn't find an MSDS. The limited information available suggests that this process may depend on microwave fixation. John Kiernan on this list has expressed many times, much more eloquently than I can, his opinion of doing science with secret ingredients. Bob Richmond Samurai Pathologist Knoxville TN From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Jul 15 05:07:40 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 15 05:08:21 2009 Subject: [Histonet] HTL Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0720ABAB@wahtntex2.waht.swest.nhs.uk> Some volunteer to stay 'behind the medics' as it is safe; some are kept there kicking and screaming (I'm hoarse). Medics are medics; it is a 'gentlemen's club' but non-Path medics are finding their position eroded by the Consultant Nurse and Consultant Physiotherapist. Pathologists are strenously opposing the idea of a Consultant Biomedical Scientist but bizzarely the Consultant Clinical Scientist is seen as OK. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: 15 July 2009 09:01 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu; Shea's Subject: Re: [Histonet] HTL well said!! your statement: 'Only when we step from behind the skirts of the Medics will the sun shine on us' deserves dissection (pardon the pun) are we volutarily 'behind the medics" or are we conveniently 'kept' there by those same medics medics=pathologists (some exceptions) where i come from most of these 'medics' are running the (very lucrative) private labs and the techs are kept 'lean and hungry' - they are 'worker bees'' grateful for work and paid a pittance. i once voiced my desire to take unpaid leave in order to study further and was refused time off for this, on the basis that i would then cost more to employ!!! i have a 4 year diploma (now called a BTech degree) - i am licensed as a Medical Technologist with Cell Path Speciality. i am neither an HT or an HTL. i have 30 years experience and have been supervising/managing AP labs for over 15 years but because i dont have a degree i would most likely have a hard time finding employment in the USA or Canada - your loss guys. its not what you call it its how you apply what you know - having a degree does not make you a good tech. flame away!! AnnieinArabia (out of Africa) 2009/7/15 Kemlo Rogerson > Do you want the blunt truth? > > There's a perception, even within the other disciplines in Diagnostic > Labs, that BMS's in Histology (HistoTechs) are second rate Scientists. > I know that's an inflammatory remark but I've battled with it for years. > Pharmacists, Physiotherapists, Ots, Audiologists and Speech Language > Therapists run Clinics treat Patients and are 'clinical'. The > perception is that 'scientists' are not clinical and before we get > appreciated for that we probably need to run Clinics ourselves but how > do Histotechs/ BMS's achieve that? In the UK scientific staff are > slowly doing that with Anticoagulant Clinics, with advanced dissection > and the reporting of cervical smears after achieving the appropriate > level of qualification. > > I'm hoping one day that the 'glass ceiling' will be taken off the Path > Labs and that a scientist will, after obtaining his/ her degree, > Masters (or PhD), like the Clinical Scientists, obtain the MRCPath and > then clinically lead a discipline. Only when we step from behind the > skirts of the Medics will the sun shine on us. > > Does that help? > > > > > > > > > Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's > Sent: 15 July 2009 04:13 > To: jaustin1967@gmail.com > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HTL > > Michael, > Ditto, very well stated. I too believe that our industry is under > appreciated. Many new grads of today find a two year degree demeaning > and wouldn't consider HT because of it. I don't understand how some > professions like pharmacy & physical therapy gain respect and grow to > create 5 yr, 6yr & 7yr programs. They are very well respected by the > MDs and Hospital administration and have nice salaries to show for it. > > Why hasn't our field flourished? > Jan, BS, HTL > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jul 15 05:22:04 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 15 05:22:26 2009 Subject: [Histonet] Eosin in Alcohol In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E10E@lmhsmail.lmhealth.org> News to me.... We have used this for many years and have never had a problem with IHC ourselves or heard of anyone else having issues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Harrison, Sandra C. Sent: Tuesday, July 14, 2009 5:15 PM To: Jennifer Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosin in Alcohol "polycyclic aromatic flourescent compounds that in high concentrations" ???? I wouldn't think the 3 mls of eosin dropped in the last 95% alcohol could be considered "high concentration" but that's what keeps Histonet entertaining; I learn something new every day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Tuesday, July 14, 2009 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin in Alcohol A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: "The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue." _________________________________________________________________ Lauren found her dream laptop. Find the PC that's right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290__________ _____________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jul 15 09:13:04 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jul 15 09:13:08 2009 Subject: [Histonet] Frozen control Message-ID: <65365F35C0F2EF4D846EC3CA73E49C437AA21CB1D8@HPEMX3.HealthPartners.int> I am resubmitting my question concerning the use of a control for the frozen section staining. Does anyone run a control and, if so, how often and do you store them in the freezer? This was a suggestion we received, but, not certain if we need or want to go this route. Any input would be greatly appreciated and thanks so much! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From shive003 <@t> umn.edu Wed Jul 15 09:18:00 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jul 15 09:18:06 2009 Subject: [Histonet] RE: human vimentin IHC References: Message-ID: <727621A859A241B881C3F19955774717@auxs.umn.edu> Just a comment from the veterinary world... in my validation tests of Vimentin (clone V9 by Dako), I found that xVimentin DOES cross-react with dog, cat, pig, cow, horse, donkey, goat, sheep, deer, chicken, rabbit, frog (weakly), and primate. Dako's data from the past stated that V9 Vimentin did cross-react with hamster and rat, but NOT with mouse. I have not tried it myself on these last three animals. So, if Dako's data is accurate, and as long as you're only working with mouse tissue as your host stromal tissue, you should be OK in using Vimentin, at least Dako's V9 Vimentin. But be sure to do your own validation on the separate species first to prove that your antibody does not bind to mouse tissue, before proceeding with the whole project. I also use heat retrieval (pressure cooker) in Dako Target Retrieval Solution for Vimentin antigen unmasking. Citrate buffer for antigen retrieval also works fine. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN ----- Original Message ----- From: "PALMER Jason (SVHM)" To: Sent: Tuesday, July 14, 2009 9:33 PM Subject: [Histonet] RE: human vimentin IHC Igor. A few years back I used Dako V9 mouse anti human vimentin to label human grafts in a mouse background. Tested it first on several mouse tissues and got no reactivity, compared to very strong reactivity in a variety of cell types in human tissue, and so am sure that it is human specific cf mouse. I used the Dako ARK to get around the mouse-on-mouse background issues and was happy with the staining obtained (although not quite as sensitive perhaps as a standard, LSAB method). I am actually about to try this again myself very soon. I used citrate AR and primary at 1:800 for my staining. Cheers, Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ------------------------------ Message: 4 Date: Tue, 14 Jul 2009 15:03:47 -0400 From: Igor Deyneko Subject: [Histonet] Human VIMENTIN and SMA IHC To: Histonet@lists.utsouthwestern.edu Message-ID: <35e16a770907141203h14ccc18bt2e3123d11c478182@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Popp.Laurie <@t> mayo.edu Wed Jul 15 09:31:58 2009 From: Popp.Laurie <@t> mayo.edu (Popp, Laurie A.) Date: Wed Jul 15 09:32:04 2009 Subject: [Histonet] RE: BS with HTL In-Reply-To: References: Message-ID: <1488342ADB74EC4E969C48BDCDE0BD98FB77CF@msgebe23.mfad.mfroot.org> I have a BA in Biology with my HT. The program that I went through refused to allow me to take the HTL because they were new and did not want their perfect pass rate stat messed with. That's ok I plan to take the HTL at some point in time soon.. Happy Wednesday, Laurie Laurie Popp, BA HT(ASCP) TACMA Shared resources Mayo Clinic Rochester, MN From victor <@t> pathology.washington.edu Wed Jul 15 09:45:29 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Jul 15 09:45:35 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0720AB46@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0720AB46@wahtntex2.waht.swest.nhs.uk> Message-ID: <4A5DEB89.2060600@pathology.washington.edu> Kemlo, You may not know who the pimp is, but you know who got screwed. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Kemlo Rogerson wrote: > "The histology world doesn't look for well qualified workers they look > for cheap labor (SIC). I have heard more than one pathologist state > that "a monkey can do our job." " > > See my other post. The retort ought to be that a Histology BMS/ > Histotech can do yours!! A honest Pathologist once told me that a good > Histotech could report 80% of what he did, you needed some medical > knowledge to maybe report the next 15% or so, Pathologists with a > speciality probably reported the next 2% or 3% and it took an expert to > deal with the top few percent. He taught me Pathology of the skin and I > was good at it; I naturally then became a Cytologist as there's no way, > without a MRCPath, that I could ever report skin biopsies. > > A Gynaecologist friend of mine once told that the Pathologist/ Histotech > (BMS) relationship was perceived by many of his colleagues to be the > last bastion of prostitution. I never figured out who was the pimp!! > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Bradley > Sent: 14 July 2009 21:50 > To: Weems, Joyce > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? > > HI all > > I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in > the early 90s and I guess I was misguided because I thought it would > open more doors for me than just an HT. I was sadly mistaken. After I > passed my test I waited 9 months for a raise and promotion (which was > just a greater title) and when I got my raise so did 2 other employees > that didn't even have or try for their certification. I spent many > nights and weekends studying and doing my stains for the test. I am > proud of my accomplishments. It is a shame that our industry does not > reconize the difference between HT and HTL. A few years back I was > working as a traveling histotech and when I tried to get a permanent > position no one wanted to hire me because I was over qualified by having > over 15 years experience and a HTL certification. > I worked hard to no avail. The histology world doesn't look for well > qualified workers they look for cheap labor. I have heard more than one > pathologist state that "a monkey can do our job." I have also worked in > a lab where they would hire someone with a GED to cut slides. A career > in histology is for the most part a dead end and there is no future. As > long as our industry doesn't respect education and experience there will > be less and less histotechs and the quality of the slides will suffer > which in turn will bring down patient care. > Just my 2 cents. > > MB proud HTL > On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce wrote: > > >> Honey... You are a mere child! There are some of us that have been in >> the business for 40+ years. I missed the grandfather approach by 7 mo >> - time that I didn't work moving from place to place with my military >> ex-husband. >> >> But I did finally get the degree and do the exam. But we're still >> around. And I'll probably be working till I'm 100!!! J:>) >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Thomas Jasper >> Sent: Tuesday, July 14, 2009 15:16 >> To: Feher, Stephen >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? >> >> Hi Steve, >> >> I've got no statistics to offer you...just an observation. I would >> say that finding an HTL, without a Bachelor's degree is akin to the >> proverbial needle in a haystack. Anyone that obtained their HTL, >> if/when they could be grandfathered in, is likely to be retired or >> close to it. First of all, most folks that went the OJT route for >> certification were eligible to sit for the HT only (to my knowledge). >> I've never met anyone with an HTL that did not have a Bachelor's as a >> pre-requisite. I've been doing histology for ~25 years. I've met >> people from all over the country and various parts of the world. >> Truth is there isn't an abundance of HTLs out there. Unlike the >> Medical Lab world, with the basic differences between MTs and MLTs, >> anatomic path does not exactly mirror that with the HTL and HT. It's >> true the MT and HTL both require a Bachelor's, but responsibilities in >> > > >> most labs, etc., generally do not hinge on someone being an HT vs. an >> > HTL. > >> A person like myself is probably more common (Bachelor's and an HT). >> Unless you know of someone in particular; that you want to hire, with >> an HTL without a Bachelor's, I wouldn't waste time trying to justify >> it. I guess the bottom line is if you want an HTL, that person will >> almost assuredly have a Bachelor's. If you want to hire someone >> without a Bachelor's that is certified (HT) you'll have better luck. >> I think having an HTL is a great thing. I honestly have never pursued >> > > >> it (though eligible) as the circumstances of my career would not have >> rewarded me for doing so. As a matter of fact some employers may look >> > > >> at it as an over-qualification, or at least no justification for >> better pay, perks or responsibility. Again, no slam to HTLs just the >> way things are, at least in my experience. >> >> If you want to hire people without a Bachelor's I would definitely >> pursue HTs. HTs have been doing a great deal of very good work for >> years in this field. And it sounds like you're viewing the Bachelor's >> > > >> thing as limiting factor more than the HTL itself. >> >> Good luck, >> Tom Jasper >> >> Thomas Jasper HT (ASCP) BAS >> Histology Supervisor >> Central Oregon Regional Pathology Services Bend, Oregon 97701 >> 541/693-2677 >> tjasper@copc.net >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, >> > > >> Stephen >> Sent: Monday, July 13, 2009 9:12 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] What percent of HTL's do not have a BS degree? >> >> I'm trying to find some solid statistics to justify being able to hire >> > > >> HTL (ASCP) candidates who do not have a Bachelor's degree. I am >> contending that requiring the candidate to have a Bachelor's degree >> will eliminate a substantial number of very qualified people. Does >> anyone have any solid references to support my position. >> >> Thanks, >> >> Steve >> >> >> Stephen A. Feher, MS, SCT (ASCP) >> >> Pathology Supervisor >> >> Catholic Medical Center >> >> 100 McGregor Street >> >> Manchester, NH 03102 >> >> 603-663-6707 >> >> sfeher@cmc-nh.org >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This email, including any attachments is the property of Catholic >> Health East and is intended for the sole use of the intended >> recipient(s). >> It may contain information that is privileged and confidential. Any >> unauthorized review, use, disclosure, or distribution is prohibited. >> If you are not the intended recipient, please reply to the sender that >> > > >> you have received the message in error, then delete this message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Jul 15 09:47:31 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 15 09:47:35 2009 Subject: [Histonet] RE: human vimentin IHC Message-ID: <953295.17306.qm@web65709.mail.ac4.yahoo.com> Jan: I enjoyed your posting and I commend you for it. This is what good professionals do, to share experiences and knowledge and this is one of the objectives of HistoNet. Ren? J. --- On Wed, 7/15/09, Jan Shivers wrote: From: Jan Shivers Subject: Re: [Histonet] RE: human vimentin IHC To: "histonet" , "PALMER Jason (SVHM)" Date: Wednesday, July 15, 2009, 10:18 AM Just a comment from the veterinary world... in my validation tests of Vimentin (clone V9 by Dako), I found that xVimentin DOES cross-react with dog, cat, pig, cow, horse, donkey, goat, sheep, deer, chicken, rabbit, frog (weakly), and primate.? Dako's data from the past stated that V9 Vimentin did cross-react with hamster and rat, but NOT with mouse.? I have not tried it myself on these last three animals. So, if Dako's data is accurate, and as long as you're only working with mouse tissue as your host stromal tissue, you should be OK in using Vimentin, at least Dako's V9 Vimentin.? But be sure to do your own validation on the separate species first to prove that your antibody does not bind to mouse tissue, before proceeding with the whole project. I also use heat retrieval (pressure cooker) in Dako Target Retrieval Solution for Vimentin antigen unmasking.? Citrate buffer for antigen retrieval also works fine. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN ----- Original Message ----- From: "PALMER Jason (SVHM)" To: Sent: Tuesday, July 14, 2009 9:33 PM Subject: [Histonet] RE: human vimentin IHC Igor. A few years back I used Dako V9 mouse anti human vimentin to label human grafts in a mouse background.? Tested it first on several mouse tissues and got no reactivity, compared to very strong reactivity in a variety of cell types in human tissue, and so am sure that it is human specific cf mouse.? I used the Dako ARK to get around the mouse-on-mouse background issues and was happy with the staining obtained (although not quite as sensitive perhaps as a standard, LSAB method).? I am actually about to try this again myself very soon.? I used citrate AR and primary at 1:800 for my staining. Cheers, Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ------------------------------ Message: 4 Date: Tue, 14 Jul 2009 15:03:47 -0400 From: Igor Deyneko Subject: [Histonet] Human VIMENTIN and SMA IHC To: Histonet@lists.utsouthwestern.edu Message-ID: ? ? ???<35e16a770907141203h14ccc18bt2e3123d11c478182@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 15 09:51:22 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 15 09:51:26 2009 Subject: [Histonet] Frozen control Message-ID: <301439.44310.qm@web65715.mail.ac4.yahoo.com> If the frozen is the "classical transoperatory" test to stain with H&E, nobody I know uses a control and I just don't see the point. Are you going to use a FFPE section that will have to dewax and process as usual?delaying the whole process? It just makes no sense. Either the?FS will be stained or not. Just my opinion (as usual!). Ren? J. --- On Wed, 7/15/09, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Frozen control To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, July 15, 2009, 10:13 AM I am resubmitting my question concerning the use of a control for the frozen section staining.? Does anyone run a control and, if so, how often and do you store them in the freezer?? This was a suggestion we received, but, not certain if we need or want to go this route.? Any input would be greatly appreciated and thanks so much! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Jul 15 10:37:48 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jul 15 10:37:55 2009 Subject: [Histonet] Frozen control Message-ID: <57BE698966D5C54EAE8612E8941D76830608929B@EXCHANGE3.huntingtonhospital.com> We do not run a control, nor have I ever at any of my previous jobs. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, July 15, 2009 7:13 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Frozen control I am resubmitting my question concerning the use of a control for the frozen section staining. Does anyone run a control and, if so, how often and do you store them in the freezer? This was a suggestion we received, but, not certain if we need or want to go this route. Any input would be greatly appreciated and thanks so much! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Jul 15 11:00:50 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Jul 15 11:03:11 2009 Subject: [Histonet] RE: human vimentin IHC In-Reply-To: <727621A859A241B881C3F19955774717@auxs.umn.edu> References: <727621A859A241B881C3F19955774717@auxs.umn.edu> Message-ID: <180534269-1247673779-cardhu_decombobulator_blackberry.rim.net-201725907-@bxe1213.bisx.prod.on.blackberry> In my experience V9 definitely cross reacts with mouse. -----Original Message----- From: Jan Shivers Date: Wed, 15 Jul 2009 09:18:00 To: histonet; PALMER Jason (SVHM) Subject: Re: [Histonet] RE: human vimentin IHC Just a comment from the veterinary world... in my validation tests of Vimentin (clone V9 by Dako), I found that xVimentin DOES cross-react with dog, cat, pig, cow, horse, donkey, goat, sheep, deer, chicken, rabbit, frog (weakly), and primate. Dako's data from the past stated that V9 Vimentin did cross-react with hamster and rat, but NOT with mouse. I have not tried it myself on these last three animals. So, if Dako's data is accurate, and as long as you're only working with mouse tissue as your host stromal tissue, you should be OK in using Vimentin, at least Dako's V9 Vimentin. But be sure to do your own validation on the separate species first to prove that your antibody does not bind to mouse tissue, before proceeding with the whole project. I also use heat retrieval (pressure cooker) in Dako Target Retrieval Solution for Vimentin antigen unmasking. Citrate buffer for antigen retrieval also works fine. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN ----- Original Message ----- From: "PALMER Jason (SVHM)" To: Sent: Tuesday, July 14, 2009 9:33 PM Subject: [Histonet] RE: human vimentin IHC Igor. A few years back I used Dako V9 mouse anti human vimentin to label human grafts in a mouse background. Tested it first on several mouse tissues and got no reactivity, compared to very strong reactivity in a variety of cell types in human tissue, and so am sure that it is human specific cf mouse. I used the Dako ARK to get around the mouse-on-mouse background issues and was happy with the staining obtained (although not quite as sensitive perhaps as a standard, LSAB method). I am actually about to try this again myself very soon. I used citrate AR and primary at 1:800 for my staining. Cheers, Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ------------------------------ Message: 4 Date: Tue, 14 Jul 2009 15:03:47 -0400 From: Igor Deyneko Subject: [Histonet] Human VIMENTIN and SMA IHC To: Histonet@lists.utsouthwestern.edu Message-ID: <35e16a770907141203h14ccc18bt2e3123d11c478182@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters! I am wondering if anyone can possibly advise good antibodies for HUMAN anti alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse stroma and in the past had a lot of cross reactivity and background issues. Does anyone know good antibodies or a clone, or has a good protocol for either??? All would be greatly appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Popp.Laurie <@t> mayo.edu Wed Jul 15 12:11:56 2009 From: Popp.Laurie <@t> mayo.edu (Popp, Laurie A.) Date: Wed Jul 15 12:11:59 2009 Subject: [Histonet] Frozen Control In-Reply-To: References: Message-ID: <1488342ADB74EC4E969C48BDCDE0BD98FB77DC@msgebe23.mfad.mfroot.org> Dorothy, We routinely ran frozen controls with our Oil Red O etc. when I was over in the clinical area. We cut the controls ourselves and only kept a 25 ct slide box in the freezer so when they were gone we cut more. That way you don't end up with old controls. Laurie Laurie Popp, BA HT(ASCP) TACMA Shared Resources, Mayo Clinic-Rochester From MadaryJ <@t> MedImmune.com Wed Jul 15 12:15:41 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Jul 15 12:16:10 2009 Subject: [Histonet] Do not assume you would not get hired without an ASCP Message-ID: Depending on where you want to work, to say you would likely not get hired without an ASCP certification is just not true. If you look at most CRO's, reference labs, pharmaceutical, biotechs, start ups and even the occasional hospital you will find thousands of folks who are working as high level managers without an ASCP certification. Why are we back on this topic again? Lee Luna who I knew personally as many of you did most of his work well before he ever sat for the HT, and he was the top histologist at AFIP, not to mention a pioneer in the field. I just think we Americans should be given more credit that we would and do in fact hire experienced people and hold experience in higher regard than the certification. Deep down we all know that is true. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Beth.Fye <@t> HCAhealthcare.com Wed Jul 15 12:49:33 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Wed Jul 15 12:49:40 2009 Subject: [Histonet] Re: HTL Message-ID: <938F8EC5A524D34EB5796E23E52781D316B00C7940@NADCWPMSGCMS05.hca.corpad.net> I've been reading the e-mails for a couple of days, and decided to put in an unbiased observation. Unbiased, because I am not a Histotech. I have worked at the same hospital for 19 years and been Manager of the Pathology department for 7 years. We have HT's , HTL's, some certified, some not, some with degrees, and some trained on the job. I have that needle in the haystack of a HTL without a 4 year degree. She is one of my most skilled techs, I also have a tech that was trained on the job (started as a transcriptionist for Pathology) who took the HT registry before the college requirement, and she is also a great tech. Personally, I don't think it is the degree that makes a good tech, but having education that directly relates to Histology. Here in Virginia, we do not have programs that offer training for Histotechs any longer, and it is needed. We are a busy hospital lab, and unfortunately don't have the time to mentor our techs properly. Many have a degree, but not formal Histology training and therefore have greater challenges preparing for the exam. I have a degree in Biology, and I am certified in Cytology, but that does not make me any type of Histotech. It is a unique field and skill that you all posess and you all should be proud, and stop the discussion of which is better. Beth A. Fye, CT (ASCP) From Maria.Katleba <@t> stjoe.org Wed Jul 15 13:16:53 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Jul 15 13:17:40 2009 Subject: [Histonet] Eosin in Alcohol In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E10E@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E10E@lmhsmail.lmhealth.org> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D0F527CD@SJSNT-SCMAIL03.stjoe.org> I wonder (with the economy so bad) that there are CHEAP eosin products out there. Could that be it?.. using of cheap 'knock off' eosins.. MK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, July 15, 2009 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosin in Alcohol News to me.... We have used this for many years and have never had a problem with IHC ourselves or heard of anyone else having issues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Harrison, Sandra C. Sent: Tuesday, July 14, 2009 5:15 PM To: Jennifer Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosin in Alcohol "polycyclic aromatic flourescent compounds that in high concentrations" ???? I wouldn't think the 3 mls of eosin dropped in the last 95% alcohol could be considered "high concentration" but that's what keeps Histonet entertaining; I learn something new every day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Tuesday, July 14, 2009 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin in Alcohol A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: "The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue." _________________________________________________________________ Lauren found her dream laptop. Find the PC that's right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290__________ _____________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St.Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Heather.D.Renko <@t> osfhealthcare.org Wed Jul 15 14:15:39 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Jul 15 14:16:06 2009 Subject: [Histonet] re: ViAS Message-ID: Anyone out there using VIAS for Quantitative Analysis of ER/PR? Please email me direct as I have a few questions. Thanks you! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From rsrichmond <@t> gmail.com Wed Jul 15 14:34:09 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jul 15 14:34:14 2009 Subject: [Histonet] Re: Frozen control Message-ID: Well, I've done frozen sections in a lot of hospitals, and I don't think I've ever seen a control slide stained, nor do I see the slightest technical or medical reason to do it. This is a purely a regulatory issue, I think - something to hire another paper--pusher to keep up with. Bob Richmond Samurai Pathologist Knoxville TN From jcline <@t> wchsys.org Wed Jul 15 14:45:00 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Jul 15 14:45:05 2009 Subject: [Histonet] nuclear bubbling Message-ID: Has anyone experienced nuclear bubbling on prostate biopsies? Joyce ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rjbuesa <@t> yahoo.com Wed Jul 15 14:58:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 15 14:58:42 2009 Subject: [Histonet] nuclear bubbling Message-ID: <694310.52101.qm@web65713.mail.ac4.yahoo.com> Anybody can experience nuclear bubbling in any type?tissue as long as the sections as set to dry?at high temperature BEFORE they are completely drained off! Ren? J. --- On Wed, 7/15/09, Joyce Cline wrote: From: Joyce Cline Subject: [Histonet] nuclear bubbling To: "Histonet" Date: Wednesday, July 15, 2009, 3:45 PM Has anyone experienced nuclear bubbling on prostate biopsies? Joyce ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandoval.1965 <@t> hotmail.com Wed Jul 15 15:35:08 2009 From: sandoval.1965 <@t> hotmail.com (Anthony Sandoval) Date: Wed Jul 15 15:35:12 2009 Subject: [Histonet] aspiring histotech Message-ID: Hello histology world! I have been working in a histology lab doing mostly IHC for over a year now and I am interested in taking the HT or HTL exam. I have a B.S. in biology so I can sit for either exam. I was wondering about the pro's and con's of each and any study material that would best help me. Thank you all for the info and this site is a great source of info and inspiration for my chosen career! Anthony _________________________________________________________________ Insert movie times and more without leaving Hotmail?. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 From disbrc <@t> shands.ufl.edu Wed Jul 15 15:37:42 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Wed Jul 15 15:38:16 2009 Subject: [Histonet] Hi. Message-ID: <4A5E05D6.72AC.0059.0@shands.ufl.edu> Hi. Hearing all these comments about histology is really depressing. I was hoping by getting my BS and upgrading my ASCP certification I would at least get, what I consider, a more interesting job. My BS will be veterinary technology, similar to a registered nurse with a bachelors but for animals. I was hoping I could work in Research or immunos, in a vet school, or university setting. Does anyone have any advice? By the way please post them to the board and not to my email address. Thanks! Carrie From cbarone <@t> NEMOURS.ORG Wed Jul 15 15:57:14 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Jul 15 15:57:26 2009 Subject: [Histonet] 2 items.. to post Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A7448@wlmmsx01.nemours.org> Histonetter's: #1- What are your thoughts on the many new sites for Histology "assistants"....they come very close to sounding like Histotechnicians....which they are not. I am not 100% on allowing another category for "assistants"...but , would like a true differentiation between "assistant", technician and technologistif it must be....since there most decidedly are differences. technicians out there...do you want to go back to being your pathologist's " assistant?" Or, are you a professional, with a title of your own, you earned! In this time when we are just begining to come from the shadows and emerg as professionals...I believe " assistants" take us back not forward in being professionally recognized. I think the development of these programs are just a quickie way for pathologists to fill the vast lost of skilled and qualified histologists that are retiring and a way to keep wages repressed...That "assistant" subset, will wish they had not taken that route father down the road. We should encourage people to enter the excellent programs we have and continue to work for higher education goals with better programs.....What are your thoughts. Would you want to be a histology "assistant"? You know where that will take us. It is merely a short-term fix for a problem that needs more than a band-aid... #2 It recently came to my attention that AFIP will close on Oct 1. Did anyone ask you, who use AFIP if that was a good idea, or explain why they think it is? Who thinks what out there?....I think my letter to the President is not enough? But, yours and mine might have some impact on that???? What do you think? It is another way to be your own best advocate for your field. Have a voice...either way...at least it is your voice. From shive003 <@t> umn.edu Wed Jul 15 16:16:42 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jul 15 16:16:47 2009 Subject: Fw: [Histonet] Hi. Message-ID: <7F87C37798DC45F0ADED4CAFB42665E3@auxs.umn.edu> Carrie, I work at a University, doing both clinical and research veterinary work. Laboratory technical staff are not required to have BS degrees here (though it's desired), but our higher level scientist positions (which would be the lead techs, lab supervisors, and section heads) must have a bachelor's degree or higher. Because of the research that most labs get involved in at my university, it's important to have a strong educational background in the various scientific fields (biology, zoology, microbiology, virology, biochemistry, anatomy, pathology, etc.) in order to understand and do proper R&D of new techniques (that's the fun part of Histo and IHC). Plus, lab managers do a fair amount of technical writing and verbal correspondences with colleagues around the globe, so having good compositional and conversational skills (to convey that knowledge) is important. This probably holds true for many other institutions as well. So, yes, get your BS degree. With it, you'll most likely go where you want to go in your career, given time and perseverance. Feel free to contact me personally, if you have any other questions. Jan Shivers Section Head Histology/Immunohistochemistry/Electron Microscopy Labs Veterinary Diagnostic Laboratory College of Veterinary Medicine University of Minnesota St. Paul, MN shive003@umn.edu ----- Original Message ----- From: "Carrie Disbrow" To: Sent: Wednesday, July 15, 2009 3:37 PM Subject: [Histonet] Hi. Hi. Hearing all these comments about histology is really depressing. I was hoping by getting my BS and upgrading my ASCP certification I would at least get, what I consider, a more interesting job. My BS will be veterinary technology, similar to a registered nurse with a bachelors but for animals. I was hoping I could work in Research or immunos, in a vet school, or university setting. Does anyone have any advice? By the way please post them to the board and not to my email address. Thanks! Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Wed Jul 15 17:24:37 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Wed Jul 15 17:25:28 2009 Subject: [Histonet] Looking for block of ARVC tissue Message-ID: <4A5DF4B5.11C6.00D6.0@mrl.ubc.ca> We are working on a project and we need to find genetically proven material from a case of Arrhythmogenic Right Ventricular Cardiomyopathy, either a block or some slides in order to compare it to material we already have available here, but which is not genetically proven to have the condition. Any info would be greatly appreciated. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From tkngflght <@t> yahoo.com Wed Jul 15 17:43:56 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jul 15 17:43:00 2009 Subject: [Histonet] experienced traveler needed for ONE WEEK Message-ID: <892B2F98C5C74A5588CFC761C68C85CD@FULLSTAFF.ORG> Hi Guys- We have an opening for experienced traveler for one week assignment, next week, day shift and most of our regulars are on other assignments. Embed and cut to facility protocols. Resumes may be submitted privately to: admin@fullstaff.org Cheryl Cheryl R. Kerry, Principal, HT(ASCP) Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time! 281.852.9457 800.756.3309 eFax From lldewe <@t> gmail.com Wed Jul 15 17:46:51 2009 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Wed Jul 15 17:46:55 2009 Subject: [Histonet] aspiring histotech In-Reply-To: References: Message-ID: <7173d3c00907151546p63d835b3nedfbd9fbe6a212a1@mail.gmail.com> I am interested in knowing this also please!! Loralei On Wed, Jul 15, 2009 at 1:35 PM, Anthony Sandoval wrote: > > Hello histology world! > > > > I have been working in a histology lab doing mostly IHC for over a year > now and I am interested in taking the HT or HTL exam. I have a B.S. in > biology so I can sit for either exam. I was wondering about the pro's and > con's of each and any study material that would best help me. Thank you all > for the info and this site is a great source of info and inspiration for my > chosen career! > > > > Anthony > > _________________________________________________________________ > Insert movie times and more without leaving Hotmail?. > > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From halsteaj <@t> ohsu.edu Wed Jul 15 18:28:26 2009 From: halsteaj <@t> ohsu.edu (Jeff Halstead) Date: Wed Jul 15 18:28:36 2009 Subject: [Histonet] (no subject) Message-ID: <6CBE81CD55E1CF4096C7DBD60C979C330324551A@EX-MB02.ohsu.edu> Hi All, We are looking into purchasing an automated microtome and would like to find out what people use and recommend Thanks From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jul 16 01:59:34 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jul 16 02:00:39 2009 Subject: [Histonet] nuclear bubbling Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0720AC66@wahtntex2.waht.swest.nhs.uk> Sub optimal fixation and as Rene said; drying off at high temperatures. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: 15 July 2009 20:45 To: Histonet Subject: [Histonet] nuclear bubbling Has anyone experienced nuclear bubbling on prostate biopsies? Joyce ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jul 16 03:18:01 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 16 03:18:09 2009 Subject: AW: [Histonet] formalin substitutes - tissue structure In-Reply-To: <33b7cf2b0907141632x7a16321bhe6d8a67287fd3bfc@mail.gmail.com> References: <33b7cf2b0907141632x7a16321bhe6d8a67287fd3bfc@mail.gmail.com> Message-ID: I have no scientific experience with this, but in my opinion it has to make a difference, if the proteins are crosslinked with single methylen-bridges or with "longer" bridges, that result of "more-C-compounds". Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Yak-Nam Wang Gesendet: Mittwoch, 15. Juli 2009 01:32 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] formalin substitutes - tissue structure Dear Histonetters, I have a question about alternatives to formalin fixation and fine changes in tissue structure. We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company. >>From the Histonet archives I see that sometimes the use of formalin alternatives can affect immuno staining, but does anyone know how it would affect fine structure of tissue. My thoughts were that there may be a slight difference in 'shrinkage' that occurs given the main ingredient is ethanol on some of the alternatives, so fat fixed in one of these alternatives would give an erroneously smaller adipose cell size. Any insight would be greatly appreciated. Thank you Yak-Nam Wang University of Washington _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GAshton <@t> picr.man.ac.uk Thu Jul 16 06:01:32 2009 From: GAshton <@t> picr.man.ac.uk (Garry Ashton) Date: Thu Jul 16 05:58:32 2009 Subject: [Histonet] GFP IHC frozen samples Message-ID: <096D992D69D1CE4E863A3758C7BDB641025AD007@PMAIL01.picr.man.ac.uk> Hi all, I spent hours and looked at several antibodies on this subject. Does anybody have a protocol that actually works for GFP staining on fresh frozen samples or is the signal simply destroyed? Many thanks -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From GenieJacobs <@t> texashealth.org Thu Jul 16 07:17:55 2009 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Thu Jul 16 07:18:01 2009 Subject: [Histonet] immuno staining Message-ID: <7D894CD1537BE943BBF97DEDAFD8B4F414E900F3@PHDEXMB03.txhealth.org> We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our immuno stains. We recently purchased the Ventana Ultra and really like it. We dual mount the control and patient. We have recently had several cases where the control was positive but the patient was not. We repeated the stain and the patient was positive the second time. We have had issues with washing but have increased the time in the oven Has anyone else had this problem We don't have a lot of variables that are different Thanks for any input The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Michael.Kerschnitzki <@t> mpikg.mpg.de Thu Jul 16 07:18:20 2009 From: Michael.Kerschnitzki <@t> mpikg.mpg.de (Michael Kerschnitzki) Date: Thu Jul 16 07:18:27 2009 Subject: [Histonet] Osteoid fluorescence staining Message-ID: Hey Histonetters, Can anybody tell me a fluorescence stain for osteoid. I need to measure the osteoid volume around/at the surface of bone lacunae with confocal laser scanning microscopy. I heard about basic fuchsin but there the lacunae voids are also stained so i guess it's hard then to distinguish between the void and the osteoid. Thanks for your help. Michael =============================================== Michael Kerschnitzki Biomaterials Department Max Planck Institute of Colloids and Interfaces Postal address: MPI KGF Golm, D - 14424 Potsdam, Germany Physical address: Am M?hlenberg 1, D - 14476 Golm Germany Telephone: +49 (331) 567 - 9464 Fax: +49 (331) 567 - 9402 Email: kerschnitzki@mpikg.mpg.de =============================================== From Dawn.Gullifer <@t> osumc.edu Thu Jul 16 07:40:46 2009 From: Dawn.Gullifer <@t> osumc.edu (Gullifer, Dawn) Date: Thu Jul 16 07:40:53 2009 Subject: [Histonet] Job Opening Columbus-Ohio Message-ID: <015143EA05CF8A4ABE0321A2AD81CEBA018CBCFD@msxc02.OSUMC.EDU> We currently have 2 openings both for an Anatomic Pathology Technologist. 1 position is Full Time from 3:00pm to 11:30pm and the other is Part Time from 4:00pm-8:00pm. Position Description: provides accessioning, gross examination for surgical specimens, creates reports of tissue gross description, prepares tissues for processing, maintains lab supplies and procurement records, provides inventory summaries and other general lab responsibilities as required. Experience and Qualifications: minimum of 2 year associates degree in science related field or minimum of 24 semester hours (36 quarter hours) of biology, chemistry, physics, and math; training or experience as a tissue prosector desired, certification or experience as a histology technologist desired, certification, training or experience as an anatomic pathology technician desired. If interested please email or fax cover letter and resume to: Attn: Dawn Gullifer Fax 614-293-2102 dawn.gullifer@osumc.edu Dawn Gullifer BS, HT (ASCP) Laboratory Manager OSU Histology Lab, LLC 614-293-0358 office 614-293-0345 lab dawn.gullifer@osumc.edu This e-mail, including attachments, may contain information that is physician-patient privileged, proprietary or otherwise confidential. If you are not the intended recipient or his or her authorized agent, use and disclosure of this message are prohibited. Any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this e-mail and immediately delete the message and any attachments. From brian <@t> prometheushealthcare.com Thu Jul 16 09:02:43 2009 From: brian <@t> prometheushealthcare.com (Brian with Prometheus) Date: Thu Jul 16 08:01:28 2009 Subject: [Histonet] Histotech Opening in New Rochelle, NY. Day Shift Message-ID: My name is Brian Feldman and I am a Principal of Prometheus Healthcare.  We are a nationwide executive search firm that specializes in the healthcare industry. We are committed to connecting dedicated healthcare professionals with top medical organizations nationwide.   I wanted to reach out to you in regards to a new position that I am currently working on   1) Medical Center in New Rochelle- immediate opening for histotechnician or histotechnologist.  Schedule is a day shift.  Hours will rotate on a weekly or monthly basis from 7am to 3pm, 8am to 4pm, or 9am to 5pm. It is Monday thru Friday.  Pay is based upon experience     If you are not currently looking we do offer generous referral bonuses if you refer someone and we are able to place them in the position.    Thanks and I look forward to hearing from you!     ****Also be sure to join us on Twitter to stay up to date on our newest lab positions**** http://twitter.com/PrometheusBlog           Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com From alyssa <@t> alliedsearchpartners.com Thu Jul 16 08:12:39 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Jul 16 08:12:44 2009 Subject: [Histonet] Histology Position In NJ Message-ID: Good Morning Histonet, Allied Search Partners is now accepting resumes for client of NJ. We are accepting the following resumes for permanent/direct hire positions: *Histotechnologists/Histotechnicians * Shifts: *Day, Evening, Night* Location: *Somerville, NJ area* Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. *Other Positions In New Jersey: *Long Branch, NJ, Denville, NJ Please submit resume to alyssa@alliedsearchpartners.com and indicate that you are interested in this position -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From gsacrylicdesigns <@t> gmail.com Thu Jul 16 08:34:56 2009 From: gsacrylicdesigns <@t> gmail.com (Golden State Acrylic Designs) Date: Thu Jul 16 08:35:08 2009 Subject: [Histonet] Biological hood with grossing station Message-ID: Is the a source for a biological hood with grossing station othe than (Thermo-Fisher) Thanks From sfeher <@t> CMC-NH.ORG Thu Jul 16 09:15:54 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Jul 16 09:17:10 2009 Subject: [Histonet] HTL and HT Thank all of you Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1C5A@exchange.cmc-nh.org> Thank all of you who posted a response to my question about BS degree's and Histotechnologist. I especially appreciate the number of responses that came from "seasoned" HTL's. Because of your impute I was able to convince my hospital and craft a job description that leaves no one out from consideration for the position. Like may of you, I have been burned in the past by having the experience but not the "required credentials" to be paid properly for the work that I was doing. Unfortunately, this is not likely to change. The success that the USA has shown in matters of QA and QC accuracy have been attributed to our certification and accreditation processes. The increased sophistication of all aspects of Histotechnology and the advent of molecular testing provide an excellent forum for all of us to lobby our certification agencies to include or create additional certifications for Histotechnologist to take part in Cytogenetic, Molecular Pathology and the like. Sure it may require some extra training or college credit but that's exactly what every other field in clinical laboratory medicine has had to do. Every time I'm exposed to another aspect of special staining or IHC it becomes clearer and clearer that the scientific knowledge is there in our technologists. We just need to rally around that point and begin to have our State and National Histology Associations to present a unified front to ASCP, CAP and the like, for more recognition (and pay) based on the tasks that are already being done. We are getting ready to post a position for a Histotechnologist (HTL) position (Manchester, New Hampshire) that will be the point person in crafting the histology laboratory procedures and process for all aspects of the lab. The expected "hire" date will be in October. We will not be taking patient specimens until February of 2010 so this person will not be engaged in "wet work" for some time. As the year progresses we will add 2 HT or above positions and 2 Path Tech (Assistant) positions as well. Thanks again for all your help. Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From anh2006 <@t> med.cornell.edu Thu Jul 16 09:39:16 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jul 16 09:40:48 2009 Subject: [Histonet] GFP IHC frozen samples In-Reply-To: <096D992D69D1CE4E863A3758C7BDB641025AD007@PMAIL01.picr.man.ac.uk> References: <096D992D69D1CE4E863A3758C7BDB641025AD007@PMAIL01.picr.man.ac.uk> Message-ID: <1563817165-1247755227-cardhu_decombobulator_blackberry.rim.net-931832976-@bxe1213.bisx.prod.on.blackberry> GFP is not destroyed in frozen sections but the tissue should be PFA fixed prior to sectioning, ideally. If you have already prepped your tissues and have no choice but to proceed with fresh frozen be aware that GFP is soluble and can leach out of fresh frozens. In addition, in fresh frozen preps it is deleterious to let them dry too much as GFP will quench when dried. I suggest you fix your tissue immediately after sectioning and just put it on the scope (after coverslipping obviously) and see what you see, and go from there. For anti-GFP use the rabbit anti-GFP from Molecular Probes/Invitrogen. It works well in frozen and paraffin, immunofluorescence and chromogenic. -----Original Message----- From: Garry Ashton Date: Thu, 16 Jul 2009 12:01:32 To: Subject: [Histonet] GFP IHC frozen samples Hi all, I spent hours and looked at several antibodies on this subject. Does anybody have a protocol that actually works for GFP staining on fresh frozen samples or is the signal simply destroyed? Many thanks -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Jul 16 09:41:42 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Jul 16 09:43:43 2009 Subject: [Histonet] Vacuum embedding. Message-ID: We have recently bought a new vacuum embedding oven but there is some dispute in the lab as to the correct vacuum pressure we should be using. I won't prejudice the replies by giving you our values, but what are the common vacuum pressures that are being used? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From annigyg <@t> gmail.com Thu Jul 16 09:50:12 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Jul 16 09:50:16 2009 Subject: [Histonet] Biological hood with grossing station In-Reply-To: References: Message-ID: there are a few out there - off the top of my head id say try MOPEC Annieinarabia - yayyy its weekend 2009/7/16 Golden State Acrylic Designs > Is the a source for a biological hood with grossing station othe than > (Thermo-Fisher) > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PHORGE1 <@t> Fairview.org Thu Jul 16 10:05:37 2009 From: PHORGE1 <@t> Fairview.org (Horge, Pamela J) Date: Thu Jul 16 10:05:44 2009 Subject: [Histonet] Rectal cancer whole mount technique Message-ID: <2CFE87A91F5ACB4DB062CF69A861F8BD069E2C2E@digsmxmbx03.Fairview.org> I recently received a request from our Cancer Center to provide whole mount evaluations of rectal cancer specimens using the "Quirke technique" and a sledge microtome. The articles I located describe in depth the role of the surgeon, pathologist and radiologist. However there was almost no information related to the equipment needed, modification to processing programs, microtomy or unique skills needed by the histologist. Is there anyone out there who has or is currently performing this technique and is willing to give me an idea what it take to implement in our lab? I would also be interested in a reference lab that would be able to perform the technical component for our pathologist to interpret either on a temporary or potentially permanent basis. Pamela Horge phorge1@fairview.org 2450 Riverside Avenue 3rd Floor East Room M340-8 Minneapolis, MN 55454 Phone: 612-273-2884 Pager: 612-899-7036 Fax: 612-273-9124 From JMitchell <@t> uwhealth.org Thu Jul 16 10:39:15 2009 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Thu Jul 16 10:39:19 2009 Subject: [Histonet] 1st Time Attendees to NSH S/C Birmingham Message-ID: <2108AECB05DBFF48A9C436A792155740011B2D74@UWHC-MAIL03.uwhis.hosp.wisc.edu> Will you be a 1st time attendee to the NSH Symposium/Convention in Birmingham, Alabama in October? Or have not attended an NSH S/C in at least 5 years? If so - you may be eligible for $500 to attend the symposium and be the recipient of one of nine $500 Newcomer-Newcomer Awards. The 4 requirements are: You are a current member of NSH, you need funding, you will utilize the funds to attend the NSH S/C within the next 2 years, you are a 1st time attendee or have not attended a NSH S/C in at least 5 years. One recipient from each of the 9 NSH regions will be chosen for $500 Newcomer-Newcomer Scholarships. Application deadline is August 1st. Apply online through the NSH website. www.nsh.org Recipients will be notified prior to the 2009 NSH S/C. Feel free to contact me if you have any questions regarding this or any of NSH Scholarships/Awards. Jean Mitchell - NSH Awards Chair From bhewlett <@t> cogeco.ca Thu Jul 16 10:50:05 2009 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu Jul 16 10:49:59 2009 Subject: [Histonet] Vacuum embedding. References: Message-ID: <86C34C6F1C9146AC9C4637619117678B@mainbox> Hi Ian, For paraffin wax infiltration, the normally quoted range of vacuum pressures to use varies between 360 mmHg(48 kPa) and 260 mmHg(34.66 kPa). I always reduced the pressure slowly in the first wax to just 360 mmHg to prevent violent 'bumping' due to rapid outgassing of the clearant. Second wax can be taken to 300 mmHg and third & fourth waxes to 260 mmHg. Cheers, Bryan ----- Original Message ----- From: "Ian Montgomery" To: Sent: Thursday, July 16, 2009 10:41 AM Subject: [Histonet] Vacuum embedding. > We have recently bought a new vacuum embedding oven but there > is > some dispute in the lab as to the correct vacuum pressure we should be > using. I won't prejudice the replies by giving you our values, but what > are > the common vacuum pressures that are being used? > > Ian. > > > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lldewe <@t> gmail.com Thu Jul 16 11:53:16 2009 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Thu Jul 16 11:53:31 2009 Subject: [Histonet] aspiring histotech In-Reply-To: References: <7173d3c00907151546p63d835b3nedfbd9fbe6a212a1@mail.gmail.com> Message-ID: <7173d3c00907160953i259b2314j512fbc9aeb60496d@mail.gmail.com> Thanks to everyone who took the time to answer. I will explore this through NSH and ASCP. It sound like certification and continuing education are the keys!! Cheers, Loralei On Thu, Jul 16, 2009 at 5:33 AM, Haynes, MaryAnne wrote: > Loralei & Anthony, > It has been a long time since I took my HTL exam, but the latest > materials from NSH and the Bancroft-Gamble book are well worth it. > Pursuing a degree and certification is the best way to advance in your > career. > I have 2 bachelors, 2 masters degrees, and a doctorate along with 4 ASCP > certifications. I am currently an operations manager for a research and > a clinical AP laboratory, soon to be advanced to an Executive Director. > I am given a salary with perks comparable to faculty and staff > physicians. > I absolutely love my profession and have never left histology behind for > career advancement, but I have certainly expanded what I do. > Our clinical AP is combined Cytology, Histology, and IHC with ISH. > Our research lab is primarily molecular procedures (PCR etc..) along > with IHC, ISH, and Laser Capture Microscopy. > Education and certification can get you wherever you want to go. > Hope this helps > > Mary Anne D. Haynes, > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Loralei > Dewe > Sent: Wednesday, July 15, 2009 18:47 > To: Anthony Sandoval > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] aspiring histotech > > I am interested in knowing this also please!! > > Loralei > > On Wed, Jul 15, 2009 at 1:35 PM, Anthony Sandoval > > wrote: > > > > > Hello histology world! > > > > > > > > I have been working in a histology lab doing mostly IHC for over a > year > > now and I am interested in taking the HT or HTL exam. I have a B.S. in > > biology so I can sit for either exam. I was wondering about the pro's > and > > con's of each and any study material that would best help me. Thank > you all > > for the info and this site is a great source of info and inspiration > for my > > chosen career! > > > > > > > > Anthony > > > > _________________________________________________________________ > > Insert movie times and more without leaving Hotmail(r). > > > > > http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tu > torial_QuickAdd_062009_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended > recipient(s) and may contain confidential and privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > > From disbrc <@t> shands.ufl.edu Thu Jul 16 15:15:24 2009 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Thu Jul 16 15:15:42 2009 Subject: [Histonet] Hi Jan, Message-ID: <4A5F521B.72AC.0059.0@shands.ufl.edu> Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie From arvidsonkristen <@t> yahoo.com Thu Jul 16 16:14:36 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Thu Jul 16 16:14:41 2009 Subject: [Histonet] Quality Stuff Message-ID: <685857.31127.qm@web65709.mail.ac4.yahoo.com> Hello, I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting?standards and corrective actions for errors in the lab.? Thank you for any input. From mpence <@t> grhs.net Thu Jul 16 16:21:00 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 16 16:21:10 2009 Subject: [Histonet] Quality Stuff In-Reply-To: <685857.31127.qm@web65709.mail.ac4.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BBD@is-e2k3.grhs.net> There is NO margin of error acceptable in mislabeling blocks or slides. I expect 100% compliance with this in my department. When you have like specimens all day like derm, you cannot make labeling errors. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, July 16, 2009 4:15 PM To: histonet Subject: [Histonet] Quality Stuff Hello, I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting?standards and corrective actions for errors in the lab.? Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 16 16:47:23 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 16 16:48:21 2009 Subject: [Histonet] Quality Stuff Message-ID: <720913.73313.qm@web65707.mail.ac4.yahoo.com> There are no acceptable "standards" for mistakes. The present tendency of implementing the "6? method" in the lab?is to precisely eliminate mistakes, not to set an "acceptable" limit. Ren? J. --- On Thu, 7/16/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Quality Stuff To: "histonet" Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting?standards and corrective actions for errors in the lab.? Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Thu Jul 16 16:48:30 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Thu Jul 16 16:48:33 2009 Subject: [Histonet] Biological hood with grossing station In-Reply-To: References: Message-ID: <143120.31950.qm@web33807.mail.mud.yahoo.com> Although I really like MOPEK, another source for the East Coast would be TBJ. ________________________________ From: Golden State Acrylic Designs To: histonet@lists.utsouthwestern.edu Sent: Thursday, July 16, 2009 9:34:56 AM Subject: [Histonet] Biological hood with grossing station Is the a source for a biological hood with grossing station othe than (Thermo-Fisher) Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Jul 16 17:42:48 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jul 16 17:44:05 2009 Subject: [Histonet] Hi Jan, In-Reply-To: <4A5F521B.72AC.0059.0@shands.ufl.edu> Message-ID: Hi Carrie, The NSH convention in Alabama will also be my first. It has only taken me 30 years to finally get to one. Assuming I don't get lost I hope to see you all there (?turn right at Honolulu, left at Los Angeles, then second exit on the left?) I'll be the short tubby man with the funny accent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Friday, 17 July 2009 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hi Jan, Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From victor <@t> pathology.washington.edu Thu Jul 16 17:49:39 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Jul 16 17:50:38 2009 Subject: [Histonet] Hi Jan, In-Reply-To: References: Message-ID: <4A5FAE83.30703@pathology.washington.edu> Tony, LOL, someone with a funny accent in Alabama. Something only a local can appreciate. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Tony Henwood wrote: > Hi Carrie, > > The NSH convention in Alabama will also be my first. > It has only taken me 30 years to finally get to one. > > Assuming I don't get lost I hope to see you all there (?turn right at > Honolulu, left at Los Angeles, then second exit on the left?) > > I'll be the short tubby man with the funny accent. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie > Disbrow > Sent: Friday, 17 July 2009 6:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hi Jan, > > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary > nursing/technology and one in histology. And I have an AA where all my > electives were biology,chemistry and micro. Then I'll have a BS in > veterinary nursing/management. I'm starting a molecular program in > January. So, I have a strong skills in courses you mentioned. I'm sure I > want to do the R & D techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field > is. Did anyone ever have a counselor in their program that explained the > different types of positions? I'm looking forward to attending the NSH > convention in October. It will be my first one! Carrie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From thecitan <@t> yahoo.com Thu Jul 16 19:37:34 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Thu Jul 16 19:37:43 2009 Subject: [Histonet] Quality Stuff In-Reply-To: <720913.73313.qm@web65707.mail.ac4.yahoo.com> References: <720913.73313.qm@web65707.mail.ac4.yahoo.com> Message-ID: <1122935475-1247791003-cardhu_decombobulator_blackberry.rim.net-1531702201-@bxe1123.bisx.prod.on.blackberry> I also run a derm lab where we gross and write cassettes. The doctors medical assistants make mistakes every week so I set up a double checking system where one tech accessions and check numbers and writes slides. Then I gross and make one final qc check. this is only possible since I have low volume - not too sure about specific setups for larger lab qc Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Thu, 16 Jul 2009 14:47:23 To: histonet; kristen arvidson Subject: Re: [Histonet] Quality Stuff There are no acceptable "standards" for mistakes. The present tendency of implementing the "6? method" in the lab?is to precisely eliminate mistakes, not to set an "acceptable" limit. Ren? J. --- On Thu, 7/16/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Quality Stuff To: "histonet" Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting?standards and corrective actions for errors in the lab.? Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcadoret <@t> amc.edu.au Thu Jul 16 21:34:51 2009 From: kcadoret <@t> amc.edu.au (karine cadoret) Date: Thu Jul 16 21:34:56 2009 Subject: [Histonet] VonKossa's calcium stain Message-ID: <001001ca0687$296b2600$7c417200$@edu.au> Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia From AnthonyH <@t> chw.edu.au Thu Jul 16 22:43:37 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jul 16 22:44:38 2009 Subject: [Histonet] VonKossa's calcium stain In-Reply-To: <001001ca0687$296b2600$7c417200$@edu.au> Message-ID: Karine, Either Hx will do, though I would not have used a haematoxylin since it will lake with the calcium forming a blue stained deposit. I would expect it to mask the silver of the von-kossa stain. I would recommend 1% neutral red, ethylene green or even a light eosin counterstain. The silver "stained" calcium deposits should then stand out quite well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karine cadoret Sent: Friday, 17 July 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VonKossa's calcium stain Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From ratliffjack <@t> hotmail.com Thu Jul 16 23:15:37 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jul 16 23:15:31 2009 Subject: [Histonet] VonKossa's calcium stain In-Reply-To: <001001ca0687$296b2600$7c417200$@edu.au> References: <001001ca0687$296b2600$7c417200$@edu.au> Message-ID: What is your tissue of interest? Why not do the Von Kossa stain first and then counterstain with MacNeal's tetrachrome. This way you employ the use of a metachromatic stain for the rest of the tissue instead of just a nuclear staining hematoxylin. Jack On Jul 16, 2009, at 9:34 PM, "karine cadoret" wrote: > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead > of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From J.P.vandenWijngaard <@t> amc.uva.nl Fri Jul 17 09:38:38 2009 From: J.P.vandenWijngaard <@t> amc.uva.nl (J.P.H.M. van den Wijngaard) Date: Fri Jul 17 09:39:50 2009 Subject: [Histonet] Protocol for fluorescence of myofibers Message-ID: Dear experts of histonet, Given my formal background (chemistry synthesis) and current experience (cardiovascular research) my question may seem either too simple of not well directed. Please forgive my inexperience in these matters. In short, I am looking for a simple method that enhances the fluorescence of the cardio myofibers. In our institution, we have constructed a special setup allowing for investigation of vessel morphology. This is carried out by infusing a fluorescent plastic that polymerizes into an organ and then serially slicing the specimen while after each slice a high resolution image is taken of the remaining bulk material. As such, we can create high resolution 3D images of the vasculature, e.g. of a heart or kidney. Recently we have extended our setup (we are using a 16mpixel cooled camera which also allows very long exposure times) and are now trying to visualize the muscle fibers of the heart. For this we use a powerled (around 400nm) and image at around 600nm which seems to generates images that show autofluorescence of either collagen or muscle (I am unsure which this may be). Given these initial promising results, I would like to visualize the muscle fibers in more detail by using a staining protocol that would allow to stain post mortem hearts. I have gathered information so far that includes the use of ALA or Bouin's solution but there may be much better protocols suitable for this problem. I appreciate all feedback, thank you in advance, Jeroen Jeroen PHM van den Wijngaard, PhD Department of Biomedical Engineering and Physics Academic Medical Center Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands Tel: +31 (20) 5668796 From mtitford <@t> aol.com Fri Jul 17 10:33:34 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jul 17 10:54:12 2009 Subject: [Histonet] NSH Meeting in Alabama Message-ID: <8CBD50B14F6AE2E-EF8-3E18@webmail-md01.sysops.aol.com> With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! Michael Titford Pathology USA Mobile AL From MadaryJ <@t> MedImmune.com Fri Jul 17 12:15:41 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Jul 17 12:15:51 2009 Subject: [Histonet] von kossa In-Reply-To: References: Message-ID: I did a von kossa day the other since it was sunny out and never thought to use a hema counterstain. I will try that next time! I use ammonia water too as a bluing agent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, July 17, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Hi Jan, (Carrie Disbrow) 2. Quality Stuff (kristen arvidson) 3. RE: Quality Stuff (Mike Pence) 4. Re: Quality Stuff (Rene J Buesa) 5. Re: Biological hood with grossing station (Joseph Saby) 6. RE: Hi Jan, (Tony Henwood) 7. Re: Hi Jan, (Victor Tobias) 8. Re: Quality Stuff (thecitan@yahoo.com) 9. VonKossa's calcium stain (karine cadoret) 10. RE: VonKossa's calcium stain (Tony Henwood) 11. Re: VonKossa's calcium stain (Jack Ratliff) 12. Protocol for fluorescence of myofibers (J.P.H.M. van den Wijngaard) 13. NSH Meeting in Alabama (mtitford@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Thu, 16 Jul 2009 16:15:24 -0400 From: "Carrie Disbrow" Subject: [Histonet] Hi Jan, To: Message-ID: <4A5F521B.72AC.0059.0@shands.ufl.edu> Content-Type: text/plain; charset=US-ASCII Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie ------------------------------ Message: 2 Date: Thu, 16 Jul 2009 14:14:36 -0700 (PDT) From: kristen arvidson Subject: [Histonet] Quality Stuff To: histonet Message-ID: <685857.31127.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting?standards and corrective actions for errors in the lab.? Thank you for any input. ------------------------------ Message: 3 Date: Thu, 16 Jul 2009 16:21:00 -0500 From: "Mike Pence" Subject: RE: [Histonet] Quality Stuff To: "kristen arvidson" , "histonet" Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3BBD@is-e2k3.grhs.net> Content-Type: text/plain; charset="iso-8859-1" There is NO margin of error acceptable in mislabeling blocks or slides. I expect 100% compliance with this in my department. When you have like specimens all day like derm, you cannot make labeling errors. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, July 16, 2009 4:15 PM To: histonet Subject: [Histonet] Quality Stuff Hello, I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting?standards and corrective actions for errors in the lab.? Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 16 Jul 2009 14:47:23 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Quality Stuff To: histonet , kristen arvidson Message-ID: <720913.73313.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 There are no acceptable "standards" for mistakes. The present tendency of implementing the "6?? method" in the lab??is to precisely eliminate mistakes, not to set an "acceptable" limit. Ren?? J. --- On Thu, 7/16/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Quality Stuff To: "histonet" Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing.?? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.?? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting??standards and corrective actions for errors in the lab.?? Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 16 Jul 2009 14:48:30 -0700 (PDT) From: Joseph Saby Subject: Re: [Histonet] Biological hood with grossing station To: Golden State Acrylic Designs , histonet@lists.utsouthwestern.edu Message-ID: <143120.31950.qm@web33807.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Although I really like MOPEK, another source for the East Coast would be TBJ. ________________________________ From: Golden State Acrylic Designs To: histonet@lists.utsouthwestern.edu Sent: Thursday, July 16, 2009 9:34:56 AM Subject: [Histonet] Biological hood with grossing station Is the a source for a biological hood with grossing station othe than (Thermo-Fisher) Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 17 Jul 2009 08:42:48 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Hi Jan, To: "Carrie Disbrow" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Carrie, The NSH convention in Alabama will also be my first. It has only taken me 30 years to finally get to one. Assuming I don't get lost I hope to see you all there (?turn right at Honolulu, left at Los Angeles, then second exit on the left?) I'll be the short tubby man with the funny accent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Friday, 17 July 2009 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hi Jan, Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 7 Date: Thu, 16 Jul 2009 15:49:39 -0700 From: Victor Tobias Subject: Re: [Histonet] Hi Jan, To: Tony Henwood Cc: Carrie Disbrow , histonet@lists.utsouthwestern.edu Message-ID: <4A5FAE83.30703@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Tony, LOL, someone with a funny accent in Alabama. Something only a local can appreciate. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Tony Henwood wrote: > Hi Carrie, > > The NSH convention in Alabama will also be my first. > It has only taken me 30 years to finally get to one. > > Assuming I don't get lost I hope to see you all there (?turn right at > Honolulu, left at Los Angeles, then second exit on the left?) > > I'll be the short tubby man with the funny accent. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie > Disbrow > Sent: Friday, 17 July 2009 6:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hi Jan, > > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary > nursing/technology and one in histology. And I have an AA where all my > electives were biology,chemistry and micro. Then I'll have a BS in > veterinary nursing/management. I'm starting a molecular program in > January. So, I have a strong skills in courses you mentioned. I'm sure I > want to do the R & D techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field > is. Did anyone ever have a counselor in their program that explained the > different types of positions? I'm looking forward to attending the NSH > convention in October. It will be my first one! Carrie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 17 Jul 2009 00:37:34 +0000 From: thecitan@yahoo.com Subject: Re: [Histonet] Quality Stuff To: "Rene J Buesa" Cc: Histonet Message-ID: <1122935475-1247791003-cardhu_decombobulator_blackberry.rim.net-1531702201-@bxe1123.bisx.prod.on.blackberry> Content-Type: text/plain; charset="utf-8" I also run a derm lab where we gross and write cassettes. The doctors medical assistants make mistakes every week so I set up a double checking system where one tech accessions and check numbers and writes slides. Then I gross and make one final qc check. this is only possible since I have low volume - not too sure about specific setups for larger lab qc Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Thu, 16 Jul 2009 14:47:23 To: histonet; kristen arvidson Subject: Re: [Histonet] Quality Stuff There are no acceptable "standards" for mistakes. The present tendency of implementing the "6?? method" in the lab??is to precisely eliminate mistakes, not to set an "acceptable" limit. Ren?? J. --- On Thu, 7/16/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Quality Stuff To: "histonet" Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing.?? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.?? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting??standards and corrective actions for errors in the lab.?? Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 17 Jul 2009 12:34:51 +1000 (EST) From: "karine cadoret" Subject: [Histonet] VonKossa's calcium stain To: Message-ID: <001001ca0687$296b2600$7c417200$@edu.au> Content-Type: text/plain; charset="US-ASCII" Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia ------------------------------ Message: 10 Date: Fri, 17 Jul 2009 13:43:37 +1000 From: "Tony Henwood" Subject: RE: [Histonet] VonKossa's calcium stain To: "karine cadoret" , Message-ID: Content-Type: text/plain; charset="us-ascii" Karine, Either Hx will do, though I would not have used a haematoxylin since it will lake with the calcium forming a blue stained deposit. I would expect it to mask the silver of the von-kossa stain. I would recommend 1% neutral red, ethylene green or even a light eosin counterstain. The silver "stained" calcium deposits should then stand out quite well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karine cadoret Sent: Friday, 17 July 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VonKossa's calcium stain Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 11 Date: Thu, 16 Jul 2009 23:15:37 -0500 From: Jack Ratliff Subject: Re: [Histonet] VonKossa's calcium stain To: karine cadoret Cc: "" Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes What is your tissue of interest? Why not do the Von Kossa stain first and then counterstain with MacNeal's tetrachrome. This way you employ the use of a metachromatic stain for the rest of the tissue instead of just a nuclear staining hematoxylin. Jack On Jul 16, 2009, at 9:34 PM, "karine cadoret" wrote: > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead > of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Fri, 17 Jul 2009 15:38:38 +0100 From: "J.P.H.M. van den Wijngaard" Subject: [Histonet] Protocol for fluorescence of myofibers To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Dear experts of histonet, Given my formal background (chemistry synthesis) and current experience (cardiovascular research) my question may seem either too simple of not well directed. Please forgive my inexperience in these matters. In short, I am looking for a simple method that enhances the fluorescence of the cardio myofibers. In our institution, we have constructed a special setup allowing for investigation of vessel morphology. This is carried out by infusing a fluorescent plastic that polymerizes into an organ and then serially slicing the specimen while after each slice a high resolution image is taken of the remaining bulk material. As such, we can create high resolution 3D images of the vasculature, e.g. of a heart or kidney. Recently we have extended our setup (we are using a 16mpixel cooled camera which also allows very long exposure times) and are now trying to visualize the muscle fibers of the heart. For this we use a powerled (around 400nm) and image at around 600nm which seems to generates images that show autofluorescence of either collagen or muscle (I am unsure which this may be). Given these initial promising results, I would like to visualize the muscle fibers in more detail by using a staining protocol that would allow to stain post mortem hearts. I have gathered information so far that includes the use of ALA or Bouin's solution but there may be much better protocols suitable for this problem. I appreciate all feedback, thank you in advance, Jeroen Jeroen PHM van den Wijngaard, PhD Department of Biomedical Engineering and Physics Academic Medical Center Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands Tel: +31 (20) 5668796 ------------------------------ Message: 13 Date: Fri, 17 Jul 2009 11:33:34 -0400 From: mtitford@aol.com Subject: [Histonet] NSH Meeting in Alabama To: histonet@lists.utsouthwestern.edu Message-ID: <8CBD50B14F6AE2E-EF8-3E18@webmail-md01.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! Michael Titford Pathology USA Mobile AL ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 21 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From sareenprashant <@t> hotmail.com Fri Jul 17 12:55:16 2009 From: sareenprashant <@t> hotmail.com (prashant sareen) Date: Fri Jul 17 12:55:26 2009 Subject: [Histonet] RE: Histonet Digest, Vol 68, Issue 21 In-Reply-To: References: Message-ID: Why dont you use NFR as counterstain for Von kassa? > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 68, Issue 21 > To: histonet@lists.utsouthwestern.edu > Date: Fri, 17 Jul 2009 10:02:55 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Hi Jan, (Carrie Disbrow) > 2. Quality Stuff (kristen arvidson) > 3. RE: Quality Stuff (Mike Pence) > 4. Re: Quality Stuff (Rene J Buesa) > 5. Re: Biological hood with grossing station (Joseph Saby) > 6. RE: Hi Jan, (Tony Henwood) > 7. Re: Hi Jan, (Victor Tobias) > 8. Re: Quality Stuff (thecitan@yahoo.com) > 9. VonKossa's calcium stain (karine cadoret) > 10. RE: VonKossa's calcium stain (Tony Henwood) > 11. Re: VonKossa's calcium stain (Jack Ratliff) > 12. Protocol for fluorescence of myofibers > (J.P.H.M. van den Wijngaard) > 13. NSH Meeting in Alabama (mtitford@aol.com) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 16 Jul 2009 16:15:24 -0400 > From: "Carrie Disbrow" > Subject: [Histonet] Hi Jan, > To: > Message-ID: <4A5F521B.72AC.0059.0@shands.ufl.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! > Carrie > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 16 Jul 2009 14:14:36 -0700 (PDT) > From: kristen arvidson > Subject: [Histonet] Quality Stuff > To: histonet > Message-ID: <685857.31127.qm@web65709.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello, > I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. > > > > > ------------------------------ > > Message: 3 > Date: Thu, 16 Jul 2009 16:21:00 -0500 > From: "Mike Pence" > Subject: RE: [Histonet] Quality Stuff > To: "kristen arvidson" , "histonet" > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3BBD@is-e2k3.grhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > There is NO margin of error acceptable in mislabeling blocks or slides. I expect 100% compliance with this in my department. When you have like specimens all day like derm, you cannot make labeling errors. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson > Sent: Thursday, July 16, 2009 4:15 PM > To: histonet > Subject: [Histonet] Quality Stuff > > > Hello, > I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Thu, 16 Jul 2009 14:47:23 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Quality Stuff > To: histonet , kristen arvidson > > Message-ID: <720913.73313.qm@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > There are no acceptable "standards" for mistakes. The present tendency of implementing the "6?? method" in the lab? is to precisely eliminate mistakes, not to set an "acceptable" limit. > Ren?? J. > > --- On Thu, 7/16/09, kristen arvidson wrote: > > > From: kristen arvidson > Subject: [Histonet] Quality Stuff > To: "histonet" > Date: Thursday, July 16, 2009, 5:14 PM > > > Hello, > I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting? standards and corrective actions for errors in the lab.? Thank you for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Thu, 16 Jul 2009 14:48:30 -0700 (PDT) > From: Joseph Saby > Subject: Re: [Histonet] Biological hood with grossing station > To: Golden State Acrylic Designs , > histonet@lists.utsouthwestern.edu > Message-ID: <143120.31950.qm@web33807.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Although I really like MOPEK, another source for the East Coast would be TBJ. > > > > > ________________________________ > From: Golden State Acrylic Designs > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, July 16, 2009 9:34:56 AM > Subject: [Histonet] Biological hood with grossing station > > Is the a source for a biological hood with grossing station othe than > (Thermo-Fisher) > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Fri, 17 Jul 2009 08:42:48 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] Hi Jan, > To: "Carrie Disbrow" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi Carrie, > > The NSH convention in Alabama will also be my first. > It has only taken me 30 years to finally get to one. > > Assuming I don't get lost I hope to see you all there (?turn right at > Honolulu, left at Los Angeles, then second exit on the left?) > > I'll be the short tubby man with the funny accent. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie > Disbrow > Sent: Friday, 17 July 2009 6:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hi Jan, > > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary > nursing/technology and one in histology. And I have an AA where all my > electives were biology,chemistry and micro. Then I'll have a BS in > veterinary nursing/management. I'm starting a molecular program in > January. So, I have a strong skills in courses you mentioned. I'm sure I > want to do the R & D techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field > is. Did anyone ever have a counselor in their program that explained the > different types of positions? I'm looking forward to attending the NSH > convention in October. It will be my first one! Carrie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 7 > Date: Thu, 16 Jul 2009 15:49:39 -0700 > From: Victor Tobias > Subject: Re: [Histonet] Hi Jan, > To: Tony Henwood > Cc: Carrie Disbrow , > histonet@lists.utsouthwestern.edu > Message-ID: <4A5FAE83.30703@pathology.washington.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Tony, > > LOL, someone with a funny accent in Alabama. Something only a local can > appreciate. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Tony Henwood wrote: > > Hi Carrie, > > > > The NSH convention in Alabama will also be my first. > > It has only taken me 30 years to finally get to one. > > > > Assuming I don't get lost I hope to see you all there (?turn right at > > Honolulu, left at Los Angeles, then second exit on the left?) > > > > I'll be the short tubby man with the funny accent. > > > > Regards > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > > Laboratory Manager & Senior Scientist > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie > > Disbrow > > Sent: Friday, 17 July 2009 6:15 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Hi Jan, > > > > > > Hi Jan, > > Thanks for your input! I have two A. S. degrees. One in veterinary > > nursing/technology and one in histology. And I have an AA where all my > > electives were biology,chemistry and micro. Then I'll have a BS in > > veterinary nursing/management. I'm starting a molecular program in > > January. So, I have a strong skills in courses you mentioned. I'm sure I > > want to do the R & D techniques but not so sure about the management! > > The other thing about a histology career is learning how vast the field > > is. Did anyone ever have a counselor in their program that explained the > > different types of positions? I'm looking forward to attending the NSH > > convention in October. It will be my first one! Carrie > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************* > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > > > This note also confirms that this email message has been > > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > > ********************************************************************** > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 8 > Date: Fri, 17 Jul 2009 00:37:34 +0000 > From: thecitan@yahoo.com > Subject: Re: [Histonet] Quality Stuff > To: "Rene J Buesa" > Cc: Histonet > Message-ID: > <1122935475-1247791003-cardhu_decombobulator_blackberry.rim.net-1531702201-@bxe1123.bisx.prod.on.blackberry> > > Content-Type: text/plain; charset="utf-8" > > I also run a derm lab where we gross and write cassettes. The doctors medical assistants make mistakes every week so I set up a double checking system where one tech accessions and check numbers and writes slides. Then I gross and make one final qc check. this is only possible since I have low volume - not too sure about specific setups for larger lab qc > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Rene J Buesa > > Date: Thu, 16 Jul 2009 14:47:23 > To: histonet; kristen arvidson > Subject: Re: [Histonet] Quality Stuff > > > There are no acceptable "standards" for mistakes. The present tendency of implementing the "6?? method" in the lab? is to precisely eliminate mistakes, not to set an "acceptable" limit. > Ren?? J. > > --- On Thu, 7/16/09, kristen arvidson wrote: > > > From: kristen arvidson > Subject: [Histonet] Quality Stuff > To: "histonet" > Date: Thursday, July 16, 2009, 5:14 PM > > > Hello, > I work in a derm lab and we do all the grossing.? We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time.? I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting? standards and corrective actions for errors in the lab.? Thank you for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 9 > Date: Fri, 17 Jul 2009 12:34:51 +1000 (EST) > From: "karine cadoret" > Subject: [Histonet] VonKossa's calcium stain > To: > Message-ID: <001001ca0687$296b2600$7c417200$@edu.au> > Content-Type: text/plain; charset="US-ASCII" > > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > > > ------------------------------ > > Message: 10 > Date: Fri, 17 Jul 2009 13:43:37 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] VonKossa's calcium stain > To: "karine cadoret" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Karine, > > Either Hx will do, though I would not have used a haematoxylin since it > will lake with the calcium forming a blue stained deposit. I would > expect it to mask the silver of the von-kossa stain. > > I would recommend 1% neutral red, ethylene green or even a light eosin > counterstain. > The silver "stained" calcium deposits should then stand out quite well. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karine > cadoret > Sent: Friday, 17 July 2009 12:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] VonKossa's calcium stain > > > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 11 > Date: Thu, 16 Jul 2009 23:15:37 -0500 > From: Jack Ratliff > Subject: Re: [Histonet] VonKossa's calcium stain > To: karine cadoret > Cc: "" > > Message-ID: > Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes > > What is your tissue of interest? Why not do the Von Kossa stain first > and then counterstain with MacNeal's tetrachrome. This way you employ > the use of a metachromatic stain for the rest of the tissue instead of > just a nuclear staining hematoxylin. > > Jack > > > On Jul 16, 2009, at 9:34 PM, "karine cadoret" > wrote: > > > Hi, > > > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > > hematoxylin (which takes 6 months to ripen) ? > > > > Also, can I simply use homemade scott's tapwater for blueing instead > > of > > using a lithium carbonate solution ? > > > > > > > > Thank you for your help, > > > > > > > > Karine Cadoret > > > > Fish health laboratory manager > > > > National Center for Marine Conservation and Resource Sustainability > > > > Newnham, TAS > > > > Australia > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Fri, 17 Jul 2009 15:38:38 +0100 > From: "J.P.H.M. van den Wijngaard" > Subject: [Histonet] Protocol for fluorescence of myofibers > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > > > Dear experts of histonet, > > Given my formal background (chemistry synthesis) and current experience (cardiovascular research) my question may seem either too simple of not well directed. Please forgive my inexperience in these matters. > > In short, I am looking for a simple method that enhances the fluorescence of the cardio myofibers. > > In our institution, we have constructed a special setup allowing for investigation of vessel morphology. This is carried out by infusing a fluorescent plastic that polymerizes into an organ and then serially slicing the specimen while after each slice a high resolution image is taken of the remaining bulk material. As such, we can create high resolution 3D images of the vasculature, e.g. of a heart or kidney. > > Recently we have extended our setup (we are using a 16mpixel cooled camera which also allows very long exposure times) and are now trying to visualize the muscle fibers of the heart. For this we use a powerled (around 400nm) and image at around 600nm which seems to generates images that show autofluorescence of either collagen or muscle (I am unsure which this may be). Given these initial promising results, I would like to visualize the muscle fibers in more detail by using a staining protocol that would allow to stain post mortem hearts. I have gathered information so far that includes the use of ALA or Bouin's solution but there may be much better protocols suitable for this problem. > I appreciate all feedback, thank you in advance, > > Jeroen > > Jeroen PHM van den Wijngaard, PhD > > Department of Biomedical Engineering and Physics > Academic Medical Center > Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands > Tel: +31 (20) 5668796 > > > > > > > > > ------------------------------ > > Message: 13 > Date: Fri, 17 Jul 2009 11:33:34 -0400 > From: mtitford@aol.com > Subject: [Histonet] NSH Meeting in Alabama > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CBD50B14F6AE2E-EF8-3E18@webmail-md01.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! > > Michael Titford > Pathology USA > Mobile AL > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 68, Issue 21 > **************************************** From dchihc <@t> yahoo.com Fri Jul 17 14:07:39 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Fri Jul 17 14:07:43 2009 Subject: [Histonet] NSH Meeting in Alabama In-Reply-To: <8CBD50B14F6AE2E-EF8-3E18@webmail-md01.sysops.aol.com> References: <8CBD50B14F6AE2E-EF8-3E18@webmail-md01.sysops.aol.com> Message-ID: <475371.55563.qm@web43503.mail.sp1.yahoo.com> I agree Michael!!! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "mtitford@aol.com" To: histonet@lists.utsouthwestern.edu Sent: Friday, July 17, 2009 10:33:34 AM Subject: [Histonet] NSH Meeting in Alabama With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Jul 17 14:37:12 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Fri Jul 17 14:37:31 2009 Subject: [Histonet] What percent of HTL's do not have a BS degree? References: <1325738360.1472911247606280478.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E2473@MAPMAIL02.bsci.bossci.com> Message-ID: Here, Here! Try getting a job with a straight BS anyway... I had to go back to school to get a marketable job and was going to go for an MLT degree until I saw the Histology program in the tech school catalogue. This is what I have always wanted to do without knowing the name for it. Besides, the phlebotomy bit makes me a bit squeemish. I can deal with anything in the gross room or morgue with no problem though. :) I love the lifetime of learning bit, and so far I have been lucky to be able to indulge my curiosity and learn more. I essentially have 3 degrees (BS Biology w/ a major in English, and an AA in Histotechnology) for my HTL, which I took because who knows what the future may hold. Besides, why pay to take both exams? Not interested in managerial stuff though, just like to rat around in the lab. I have to order supplies and keep up the paperwork. That is plenty for me thanks. I like to get my hands dirty. (darn Schiffs) I have actually known a few people who have worked as histologists while waiting to get into med school. Doesn't sound dead-end to me. There are many ways histology can be used as a starting place for other jobs with more ceiling space. Creativity and ambition are the keys. (and a bit more hard work) Nothing worthwhile is ever easy. Claire P.S. Anyone about to nit-pic my grammar, I have a English Major with an emphasis in Literature. Never could diagram sentences. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Wahlberg, Nikki Sent: Tue 7/14/2009 6:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen as a career. The hospitals as well as the doctors are also to blame. I am very proud to have a B.S. and A.S.S. degree and an HTL certification. I would really like to see a monkey do my job and still achieve the high GLP standards and high quality of work that is required to get medical devices approved for human use. It makes me sad to hear people say that this is just a job not a career. I do not believe that anyone should be allowed to just come off the street and do our job. It up to us as a community to demand that institutions require certification and recognize our educations. I don't know about anyone else out there but my education cost me a lot of money and will keep me in debt for many years. I didn't waste all that money on "just a job" this is my career and I am very proud of the work I do. Nikki From Sandra.Harrison3 <@t> va.gov Fri Jul 17 16:27:01 2009 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Jul 17 16:27:35 2009 Subject: [Histonet] nuclear bubbling In-Reply-To: <694310.52101.qm@web65713.mail.ac4.yahoo.com> References: <694310.52101.qm@web65713.mail.ac4.yahoo.com> Message-ID: How about microwaving to dry slides? Can that cause nuclear bubbling? When you say completely drained off, does that mean your slides have to be completely dry prior to placing them in the oven? Thanks, Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 15, 2009 2:59 PM To: Histonet; Joyce Cline Subject: Re: [Histonet] nuclear bubbling Anybody can experience nuclear bubbling in any type?tissue as long as the sections as set to dry?at high temperature BEFORE they are completely drained off! Ren? J. --- On Wed, 7/15/09, Joyce Cline wrote: From: Joyce Cline Subject: [Histonet] nuclear bubbling To: "Histonet" Date: Wednesday, July 15, 2009, 3:45 PM Has anyone experienced nuclear bubbling on prostate biopsies? Joyce ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbowden <@t> ucsd.edu Fri Jul 17 17:02:12 2009 From: kbowden <@t> ucsd.edu (kbowden) Date: Fri Jul 17 17:03:09 2009 Subject: [Histonet] Fat histology Message-ID: <4A60F4E4.8040707@ucsd.edu> I work with bone and connective tissue. I have just been asked to process and section some fat. I would like to get some information on how to work with fat. Any information will be very helpful. -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER./* From Timothy.Morken <@t> ucsfmedctr.org Fri Jul 17 17:19:57 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Jul 17 17:20:18 2009 Subject: [Histonet] NSH Meeting in Alabama In-Reply-To: <475371.55563.qm@web43503.mail.sp1.yahoo.com> References: <8CBD50B14F6AE2E-EF8-3E18@webmail-md01.sysops.aol.com> <475371.55563.qm@web43503.mail.sp1.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C680CC288@EXMBMCB15.ucsfmedicalcenter.org> Bless your li'l ol' hearts y'all, we wouldn't dream of it! However, I will accustom myself to grits and okra before leaving for Birmingham! Tim Morken UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Friday, July 17, 2009 12:08 PM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH Meeting in Alabama I agree Michael!!! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "mtitford@aol.com" To: histonet@lists.utsouthwestern.edu Sent: Friday, July 17, 2009 10:33:34 AM Subject: [Histonet] NSH Meeting in Alabama With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From macveigh <@t> usc.edu Fri Jul 17 20:18:15 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Jul 17 20:18:04 2009 Subject: [Histonet] Fat histology Message-ID: <004c01ca0745$a0484870$5c237d80@DFS66DD1> Hi Karen, I had the same problem recently. I got very good advise trough the histonet for working with fat. You might want to check the arhives. In my case, I was in a big time crunch with this dog fat, so after all the fat was collected and stored for a while in 10% NBF I did'nt have any time to post fix it, I just had to put it in the processor. It came out great! I used 1 hour in all steps and the steps are: 1 Formalin 1 hour 2 80% 1 hour 3 95% 1 hour 4 95% 1 hour 5 100% 1 hour 6 100% 1 hour 7 100 % 1 hour 8 Clear Rite 1 hour 9 Clear Rite 1 hour 10 Clear Rite 1 hour 11 Paraffin 1 hour 12 Paraffin 1 hour 13 Paraffin 1 hour 14 Paraffin 1 hour I did it again few days ago (the same way) and again had great result. We also did IF on this fat and it worked great as well. Good luck with it Michelle Research Specialist USC Keck School of Medicine Los Angeles, CA From jkiernan <@t> uwo.ca Fri Jul 17 23:29:36 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jul 17 23:29:42 2009 Subject: [Histonet] VonKossa's calcium stain. Counterstain etc Message-ID: Any haemalum will do, but blue is not the most pleasing contrast to go with the black silver deposits. Pink or light red is better. Here are three pink to red counterstains, all traditional. Check in a textbook or manual for instructions. 1. Neutral red (CI 50040) is good: 0.5% in water; adjust to pH4 with acetic acid; stain for about 2 minutes; the solution keeps for at least 5 years and can be used repeatedly. 2. Safranine O (CI 50240) can be used similarly but needs a longer staining time. 3. Nuclear fast red (CI 60760) is also OK: NFR 0.2G, aluminium sulphate crystals 10G, water 200ml; heat until it boils, cool overnight, decant and filter; stain for 5-10min. The solution is good for about a year; always filter before using. The Biological Stain Commission has standards for certification of all three of these dyes. Certified neutral red and safranine O have been available for many (50+) years. Nuclear fast red was only recently added to the BSC's list. See Frank et al 2007. Certification procedures for nuclear fast red (Kernechtrot), C.I. 60760. Biotechnic & Histochemistry 82: 35-39. Certified NFR powder may not yet be available to labs or to vendors of stain solutions. If you buy a ready-made solution of any dye you should choose one that was made from a BSC-certified batch of the powder. To any vendors of dye powders who read this message: email me for more information about the certification criteria for nuclear fast red. Also, check out http://www.biologicalstaincommission.org and click on one of the "Vendors" tabs or links. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: karine cadoret Date: Thursday, July 16, 2009 22:36 Subject: [Histonet] VonKossa's calcium stain To: histonet@lists.utsouthwestern.edu > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing > instead of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jul 18 09:08:19 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 18 09:08:24 2009 Subject: [Histonet] nuclear bubbling Message-ID: <167542.91084.qm@web65715.mail.ac4.yahoo.com> Exactly! The sections cannot have any water remaining between them and the slide. That is the thing. Microwaving them?in that condition (with water underneath)?will also produce the same artifact. Ren? J. --- On Fri, 7/17/09, Harrison, Sandra C. wrote: From: Harrison, Sandra C. Subject: RE: [Histonet] nuclear bubbling To: "Histonet" Date: Friday, July 17, 2009, 5:27 PM How about microwaving to dry slides?? Can that cause nuclear bubbling?? When you say completely drained off, does that mean your slides have to be completely dry prior to placing them in the oven? Thanks, Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 15, 2009 2:59 PM To: Histonet; Joyce Cline Subject: Re: [Histonet] nuclear bubbling Anybody can experience nuclear bubbling in any type?tissue as long as the sections as set to dry?at high temperature BEFORE they are completely drained off! Ren? J. --- On Wed, 7/15/09, Joyce Cline wrote: From: Joyce Cline Subject: [Histonet] nuclear bubbling To: "Histonet" Date: Wednesday, July 15, 2009, 3:45 PM Has anyone experienced nuclear bubbling on prostate biopsies? Joyce ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sat Jul 18 14:30:09 2009 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Jul 18 14:30:25 2009 Subject: [Histonet] nuclear bubbling Message-ID: <928124353.20090718233009@mail.ru> Sandra: The volume of water under the section onto slide are very small for microwave heating and this water cannot be heated adequately. Paraffin is transparent for microwave and therefore instead drying of slides you will have a damaged magnetron. Never dry paraffin sections in MWO! Maxim Peshkov, Russia, Taganrog. --- On Fri, 7/17/09, Harrison, Sandra C. wrote: From: Harrison, Sandra C. Subject: RE: [Histonet] nuclear bubbling To: "Histonet" Date: Friday, July 17, 2009, 5:27 PM How about microwaving to dry slides? Can that cause nuclear bubbling?? When you say completely drained off, does that mean your slides have to be completely dry prior to placing them in the oven? Thanks, Sandy mailto:Maxim_71@mail.ru From o.isaac24 <@t> yahoo.com Sun Jul 19 09:30:14 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Sun Jul 19 09:30:20 2009 Subject: [Histonet] IHC/HISTOTECH POSITION WANTED Message-ID: <217311.14874.qm@web111614.mail.gq1.yahoo.com> Hi, ?? I am looking for a new Histotech/IHC position. I am HTL(ASCP) certified. I am open to relocation. ?Isaac. From o.isaac24 <@t> yahoo.com Sun Jul 19 09:30:00 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Sun Jul 19 09:31:07 2009 Subject: [Histonet] IHC/HISTOTECH POSITION WANTED Message-ID: <794707.86560.qm@web111615.mail.gq1.yahoo.com> Hi, ?? I am looking for a new Histotech/IHC position. I am HTL(ASCP) certified. I am open to relocation. ?Isaac. From cfitz <@t> telus.net Sun Jul 19 22:11:10 2009 From: cfitz <@t> telus.net (Cathy) Date: Sun Jul 19 22:12:12 2009 Subject: [Histonet] immuno staining In-Reply-To: <7D894CD1537BE943BBF97DEDAFD8B4F414E900F3@PHDEXMB03.txhealth.org> References: <7D894CD1537BE943BBF97DEDAFD8B4F414E900F3@PHDEXMB03.txhealth.org> Message-ID: We were having a similar issue with our IHC staining on the Ventana Benchmark Classic. We were able to track the problem down to the slides. We never found out what was wrong with the slides but as soon as we changed lot numbers the staining issue cleared up. We were able to track down the problem with the assistance of our Ventana technical rep. Through her we were able to find out that several institutions across Canada were experiencing similar staining problems. We were all using the same lot number of slides. She also informed me that there was one manufacturer of the plus slides and they were sold under multiple vendors. Good luck with tracking down your problem. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, British Columbia Canada -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacobs, Genie Sent: Thursday, July 16, 2009 5:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] immuno staining We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our immuno stains. We recently purchased the Ventana Ultra and really like it. We dual mount the control and patient. We have recently had several cases where the control was positive but the patient was not. We repeated the stain and the patient was positive the second time. We have had issues with washing but have increased the time in the oven Has anyone else had this problem We don't have a lot of variables that are different Thanks for any input The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abijag76 <@t> yahoo.co.in Mon Jul 20 06:28:14 2009 From: abijag76 <@t> yahoo.co.in (abi jag) Date: Mon Jul 20 06:29:17 2009 Subject: [Histonet] Any help for this oil red o paper Message-ID: <74424.78852.qm@web95103.mail.in2.yahoo.com> Dear histonetters, Can anybody will help me with the following oil red o paper from Charles Churukian. Journal of Histotechnology, 22(4):309-311 Charles Churukian, Lillie's Oil Red O method for neutral lipids. I am not able to access the NSH site,the following error message is coming. ? (ERROR: Specified ROOT dir [/var/www/html/live/tools/htdocs/newsite_templates/] is not a directory) Thanks abijag Looking for local information? Find it on Yahoo! Local http://in.local.yahoo.com/ From kmerriam2003 <@t> yahoo.com Mon Jul 20 07:46:15 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Jul 20 07:47:34 2009 Subject: [Histonet] anyone tried to get onto the NSH website today? Message-ID: <144860.37710.qm@web50307.mail.re2.yahoo.com> Good morning everyone, I have tried several times to get onto the NSH website today and I keep getting an error.? I am wondering if it is?down or if there is something wrong on my end. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From alyssa <@t> alliedsearchpartners.com Mon Jul 20 09:19:26 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Jul 20 09:20:25 2009 Subject: [Histonet] Part Time Position In Surprise, AZ Message-ID: Good Morning All, I have an immediate opening for a Histotechnician/Histotechnologist candidate in Surprise, AZ. The position would require the qualified candidate to work 2 days/week. 7:30am-5:00pm. HT ASCP is required, and 2+ years experience required as well. To apply please send an updated copy of your resume for prescreening purposes, *All inquires are kept confidential* following resume submitted you will be contacted to set up an initial phone screen. Thank you in advance for any inquiries. :-) -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From sbreeden <@t> nmda.nmsu.edu Mon Jul 20 09:21:31 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Jul 20 09:21:36 2009 Subject: [Histonet] NSH Website Down? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E469AF@nmdamailsvr.nmda.ad.nmsu.edu> I just tried the NSH website, and it seems to be down so it must not be us... J I'm sure someone has contacted them. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From jkiernan <@t> uwo.ca Mon Jul 20 11:25:05 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Jul 20 11:25:10 2009 Subject: [Histonet] Any help for this oil red o paper In-Reply-To: <74424.78852.qm@web95103.mail.in2.yahoo.com> References: <74424.78852.qm@web95103.mail.in2.yahoo.com> Message-ID: Try http://www.urmc.rochester.edu/path/zqu/StainsManual/index.html?OILREDOMETHODFORFATS This is from the latest version of Chuck Churukian's lab manual; I think the method is the same as in his 1999 paper in J. Histotechnol. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: abi jag Date: Monday, July 20, 2009 7:30 Subject: [Histonet] Any help for this oil red o paper To: histonet@lists.utsouthwestern.edu > Dear histonetters, > > Can anybody will help me with the following oil red o paper from > Charles Churukian. > > Journal of Histotechnology, 22(4):309-311 Charles Churukian, > Lillie's Oil Red O method for neutral lipids. > I am not able to access the NSH site,the following error message > is coming. > > (ERROR: Specified ROOT dir > [/var/www/html/live/tools/htdocs/newsite_templates/] is not a > directory) > Thanks > > abijag > > > > > > Looking for local information? > Find it on Yahoo! Local http://in.local.yahoo.com/ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From freidac <@t> sbcglobal.net Mon Jul 20 11:34:15 2009 From: freidac <@t> sbcglobal.net (Freida Carson) Date: Mon Jul 20 11:34:19 2009 Subject: [Histonet] anyone tried to get onto the NSH website today? Message-ID: <451702.30245.qm@web82502.mail.mud.yahoo.com> As of 11:30 CDT the NSH site is up and running or at least I did not have a problem getting it to come up. ? Freida Carson --- On Mon, 7/20/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] anyone tried to get onto the NSH website today? To: "Histonet" Date: Monday, July 20, 2009, 7:46 AM Good morning everyone, I have tried several times to get onto the NSH website today and I keep getting an error.? I am wondering if it is?down or if there is something wrong on my end. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Mon Jul 20 12:10:12 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Jul 20 12:10:30 2009 Subject: [Histonet] marilyn weiss will be out of the office Message-ID: I will be out of the office starting 07/20/2009 and will not return until 07/21/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. From bob.nienhuis <@t> gmail.com Mon Jul 20 12:37:07 2009 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Mon Jul 20 13:08:45 2009 Subject: [Histonet] Fluorescent dyes and xylene Message-ID: <45109da50907201037p57789551l44864cbe3f6d5edf@mail.gmail.com> Will the Alexa Fluor dyes withstand some dehydration and clearing in alcohol and xylene? If they won't are there any bright fluorescent dyes that can be obtained congugated to a secondary antibody that will? Looking to immunostain tyrosine hydroxylase in rodent brain with a fluorescent marker in tissue that needs to be dehydrated and cleared, perhaps with an abbreviated series. Bob Nienhuis VA / UCLA Neurobiology Research From leiker <@t> buffalo.edu Mon Jul 20 13:25:58 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Jul 20 13:26:07 2009 Subject: [Histonet] Fluorescent dyes and xylene In-Reply-To: <45109da50907201037p57789551l44864cbe3f6d5edf@mail.gmail.com> References: <45109da50907201037p57789551l44864cbe3f6d5edf@mail.gmail.com> Message-ID: <6D851C7E69797234A3420749@CDYwxp1931.ad.med.buffalo.edu> So you are saying that you cannot just mount the brain tissue in aqueous mounting medium at the end (after washing the excess secondary antibody)? You must dehydrate and clear before mounting (in a permament medium)? Just curious... Merced --On Monday, July 20, 2009 10:37 AM -0700 Bob Nienhuis wrote: > Will the Alexa Fluor dyes withstand some dehydration and clearing in > alcohol and xylene? > > If they won't are there any bright fluorescent dyes that can be obtained > congugated to a secondary antibody that will? > > Looking to immunostain tyrosine hydroxylase in rodent brain with > a fluorescent marker in tissue that needs to be dehydrated and > cleared, perhaps with an abbreviated series. > > Bob Nienhuis > VA / UCLA Neurobiology Research > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From ktuttle <@t> umm.edu Mon Jul 20 13:26:57 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Mon Jul 20 13:27:20 2009 Subject: [Histonet] HELP Starting a lab Message-ID: <4A647EB0.90CE.001A.3@umm.edu> I have a friend who is a Pathologist and starting a new lab. She sent me a list of start up supplies and equipment that she needs to purchase. Can I get some input on whats missing from her list, I've added a few things but Im going blank. Its a hisology lab for a urology practice if that helps. Thank you in advance! PLEASE VENDORS DONT CALL OR EMAL ME Equipment: Negative pressure hood for grossing of specimens (grossing station) Refrigerator w/freezer or separate freezer Cassette labeler Tissue processor Embedding Stations Microtomes Waterbaths, 1 per microtome Automatic H&E stainer A scale and a mixing/heating plate Manual staining dishes, and slide holders that fit in them. Slide and block storage system Supplies: Safety equipment like goggles and sleeve covers, aprons, a flame cabinet for flammable liquids acid cabinet? Blades Forceps Rulers Hemostats Scissors Glassware like flasks, beakers, graduated cylinders, and a funnel.Pipettes and tips. Reagents: Formalin 70% etoh 95% etoh 100% etoh xylene paraffin Hematoxylin stain Eosin stain Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From bob.nienhuis <@t> gmail.com Mon Jul 20 13:22:50 2009 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Mon Jul 20 13:32:56 2009 Subject: [Histonet] Re: Fluorescent dyes and xylene In-Reply-To: <45109da50907201037p57789551l44864cbe3f6d5edf@mail.gmail.com> References: <45109da50907201037p57789551l44864cbe3f6d5edf@mail.gmail.com> Message-ID: <45109da50907201122t30fd10b1jf5ab40066693f714@mail.gmail.com> Sorry, I should have mentioned that this will be done on thick frozen sections. Bob On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis wrote: > Will the Alexa Fluor dyes withstand some dehydration and clearing in > alcohol and xylene? > > If they won't are there any bright fluorescent dyes that can be obtained > conjugated to a secondary antibody that will? > > Looking to immunostain tyrosine hydroxylase in rodent brain with > a fluorescent marker in tissue that needs to be dehydrated and > cleared, perhaps with an abbreviated series. > > Bob Nienhuis > VA / UCLA Neurobiology Research > > From baderbo <@t> gmail.com Mon Jul 20 15:24:44 2009 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Mon Jul 20 15:24:49 2009 Subject: [Histonet] Re: Fluorescent dyes and xylene In-Reply-To: <45109da50907201122t30fd10b1jf5ab40066693f714@mail.gmail.com> References: <45109da50907201037p57789551l44864cbe3f6d5edf@mail.gmail.com> <45109da50907201122t30fd10b1jf5ab40066693f714@mail.gmail.com> Message-ID: <3c7e700907201324v1bd7b60cv2c4e5e073e6e1848@mail.gmail.com> Hello My experience is that most Immunofluorescence dyes do not withstand alcohol and organic solvents dehydration. You can use aqueous mounting medium with anti-fading agent. Frozen brain tissue is tough to work with; since it contains tons of lipids. Lots of mounting mediums will produce air bubbles because aqueous mounting medium being hydrophilic and brain tissue being hydrophobic. We are in the process of developing an aqueous mounting medium with anti-fading agent and should be OK to use with tissue like brain, if interested please let us know we can send you a sample for evaluation. Bader On Mon, Jul 20, 2009 at 11:22 AM, Bob Nienhuis wrote: > Sorry, I should have mentioned that this will be done on thick frozen > sections. > > Bob > > On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis >wrote: > > > Will the Alexa Fluor dyes withstand some dehydration and clearing in > > alcohol and xylene? > > > > If they won't are there any bright fluorescent dyes that can be obtained > > conjugated to a secondary antibody that will? > > > > Looking to immunostain tyrosine hydroxylase in rodent brain with > > a fluorescent marker in tissue that needs to be dehydrated and > > cleared, perhaps with an abbreviated series. > > > > Bob Nienhuis > > VA / UCLA Neurobiology Research > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Gr??en / With Kind Regards / avec l'aimable ce qui concerne / ?????? Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: baderbo@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitaIBSC@aol.com From rosenfeldtek <@t> hotmail.com Mon Jul 20 18:40:31 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Jul 20 18:40:36 2009 Subject: [Histonet] HELP Starting a lab In-Reply-To: <4A647EB0.90CE.001A.3@umm.edu> References: <4A647EB0.90CE.001A.3@umm.edu> Message-ID: Pretty good list. Don't forget first aid kits and spill kits. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Mon, 20 Jul 2009 14:26:57 -0400 > From: ktuttle@umm.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HELP Starting a lab > > I have a friend who is a Pathologist and starting a new lab. She sent me a list of start up supplies and equipment that she needs to purchase. Can I get some input on whats missing from her list, I've added a few things but Im going blank. Its a hisology lab for a urology practice if that helps. Thank you in advance! > > PLEASE VENDORS DONT CALL OR EMAL ME > > Equipment: > Negative pressure hood for grossing of specimens (grossing station) > Refrigerator w/freezer or separate freezer > Cassette labeler > Tissue processor > Embedding Stations > Microtomes > Waterbaths, 1 per microtome > Automatic H&E stainer > A scale and a mixing/heating plate > Manual staining dishes, and slide holders that fit in them. > Slide and block storage system > > Supplies: > Safety equipment like goggles and sleeve covers, aprons, a flame cabinet for flammable liquids > acid cabinet? > Blades > Forceps > Rulers > Hemostats > Scissors > Glassware like flasks, beakers, graduated cylinders, and a funnel.Pipettes and tips. > > Reagents: > Formalin > 70% etoh > 95% etoh > 100% etoh > xylene > paraffin > Hematoxylin stain > Eosin stain > > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > Please consider the environment before printing this e-mail. > > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live? Hotmail?: Search, add, and share the web?s latest sports videos. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGLM_WL_QA_HM_sports_videos_072009&cat=sports From rosenfeldtek <@t> hotmail.com Mon Jul 20 18:42:27 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Jul 20 18:42:31 2009 Subject: [Histonet] Celloidin Sections. In-Reply-To: <4A647EB0.90CE.001A.3@umm.edu> References: <4A647EB0.90CE.001A.3@umm.edu> Message-ID: I am curious--How thin can celloidin sections be cut? I've heard that 30 microns is a standard thickness. No way to make 5 micron sections? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Windows Live? SkyDrive?: Store, access, and share your photos. See how. http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009 From Barry.R.Rittman <@t> uth.tmc.edu Mon Jul 20 19:14:58 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Jul 20 19:19:24 2009 Subject: [Histonet] Celloidin Sections. In-Reply-To: References: <4A647EB0.90CE.001A.3@umm.edu>, Message-ID: <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA07@UTHCMS1.uthouston.edu> Jerry depends on whether you are talking about celloidin or Low Viscosity Nitrocelulose. There are techniques in the literature to cut celloidin sections as thin as 4 microns. For LVN you need much thicker sections as this plastic is much less sturdy and has a tendency to fragment if too thin. The questions I ask is why you need celloidin sections and what tissue are you thinking about for this technique? Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R [rosenfeldtek@hotmail.com] Sent: Monday, July 20, 2009 6:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Celloidin Sections. I am curious--How thin can celloidin sections be cut? I've heard that 30 microns is a standard thickness. No way to make 5 micron sections? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Windows Live? SkyDrive?: Store, access, and share your photos. See how. http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.malcontenti-wilson <@t> unimelb.edu.au Mon Jul 20 19:53:06 2009 From: c.malcontenti-wilson <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Mon Jul 20 19:53:38 2009 Subject: [Histonet] double immunostaining with antigen retrieval and no antigen retrieval Message-ID: <452655D3CC46464485FE5107C8723F2A05AFB973@IS-EX-BEV1.unimelb.edu.au> Dear All, Is it possible to do double immunostaining for one antibody which doesn't require antigen retrieval, and the other one which doesn't need it. Can you do the one without retrieval first, then retrieve and do the other one, or does the retrieval damage the first staining? We know that doing antigen retrieval for antibody that doesn't require it gives us non specific staining. I would appreciate some advice. Thank you very much. Cathy Malcontenti-Wilson From nyilmaz <@t> mersin.edu.tr Tue Jul 21 02:36:57 2009 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Tue Jul 21 02:37:27 2009 Subject: [Histonet] donation request Message-ID: <000601ca09d6$0b659b20$2101a8c0@nejat1> Dear Colleagues, We are expecting a quite busy period in terms of research studies. As some of you do also experiencing similiar issues, we are using fluorescent microscopes from other depts when available. But mostly it is not easy. Buying a new one does not seem possible soon due to financial issues. My question is, if any colleague has a redundant fluorescein microscope available to donate us. Your kindly helps would be greatly appreciated. Thanks in advance. Dr. Necat Yilmaz MD, PhD University of Mersin School of Medicine Histology and Embryology Dept. Mersin/TURKEY From abijag76 <@t> yahoo.co.in Tue Jul 21 07:10:23 2009 From: abijag76 <@t> yahoo.co.in (abi jag) Date: Tue Jul 21 07:10:32 2009 Subject: [Histonet] Snap freezing_method by John Tarpley_reg Message-ID: <762722.45283.qm@web95103.mail.in2.yahoo.com> Dear Histonetters, This query is with regard to snap freezing technique of rodent liver for cryotomy. Thanks a lot for the extensive details available with our forum especially from Gayle Callis. But I have seen numerous quotes regarding the use of the method from John Tarpley, who published his method in the magazine microscopy today, june 2001 issue. This issue is no longer available and I have already written to Cambridge publishers. In the mean time, if any of our histotechs having this article with them to share me a copy or any body able to give me the contact details of the author John Tarpley, so that I can contact him for a reprint Thanks for all your help Abijag Love Cricket? Check out live scores, photos, video highlights and more. Click here http://cricket.yahoo.com From MElliott <@t> mrl.ubc.ca Tue Jul 21 12:53:54 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Jul 21 12:54:35 2009 Subject: [Histonet] MMP-2 antibodies Message-ID: <4A659E42020000D60004158F@mail.mrl.ubc.ca> I am looking for some advice as to which MMP-2 antibody people recommend for staining human tissue-either FFPE or frozen sections. I have tried one from Novus as well as Serotec with no luck. I looked in the Archives but couldn't find any. Any suggestions greatly appreciated. Mark >>> abi jag 7/21/2009 5:10 AM >>> Dear Histonetters, This query is with regard to snap freezing technique of rodent liver for cryotomy. Thanks a lot for the extensive details available with our forum especially from Gayle Callis. But I have seen numerous quotes regarding the use of the method from John Tarpley, who published his method in the magazine microscopy today, june 2001 issue. This issue is no longer available and I have already written to Cambridge publishers. In the mean time, if any of our histotechs having this article with them to share me a copy or any body able to give me the contact details of the author John Tarpley, so that I can contact him for a reprint Thanks for all your help Abijag Love Cricket? Check out live scores, photos, video highlights and more. Click here http://cricket.yahoo.com ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Sean.Taheri <@t> regeneron.com Tue Jul 21 12:54:24 2009 From: Sean.Taheri <@t> regeneron.com (Sean Taheri) Date: Tue Jul 21 12:55:42 2009 Subject: [Histonet] Preventing livers from falling apart after dab stain Message-ID: HI: I have a currently removing o OCT. I cryostat these livers until used. We fix thes antibodies and DAB for histochemist that my tissue almost looks expanded. Sm magnified and it looks as if chunks of the tissue just leaving gaps in between what looks like cell clusters. Th not there when I cut or during -80 storage because I check the slides before and after freezin, and fixation. I would greatly appreciate Tha Sean Taheri for use by the addressee(s) named above and may contain legally privi leged and/or confidential information. If you are not the intended recipi of this emai If you receive this return electronic mail and p attachment hereto, any copy of this e- attachment, and any printout thereof. Finally, pleas only authorized representatives of Regeneron Pharmaceuticals, I have the power and authority to enter into business dealings with any third party. From rosenfeldtek <@t> hotmail.com Tue Jul 21 13:24:06 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Jul 21 13:24:11 2009 Subject: [Histonet] Celloidin Sections. In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA07@UTHCMS1.uthouston.edu> References: <4A647EB0.90CE.001A.3@umm.edu>, <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA07@UTHCMS1.uthouston.edu> Message-ID: Hi Barry, I am studying the development of atherosclerotic lesions in mouse brachiocephalic artery--I typically use 5 micron sections. Paraffin sections have given me mostly good, frequently excellent and occasionally spectacular results, morphology-wise. Oh, and occasionally poor results, too. I would prefer to increase the ration of spectacular/merely OK results. I read an article where the authors describe getting better fine morphology with celloidin than paraffin. Atheromas, like cochlea are delicate structures with fine detail, and they can easily become detached from the artery wall. After reading the article, I can't help but wonder if a different embedding media might give me even better results than I am already getting. Would I need a special microtome for celloidin? One reason I don't want to go with methacrylate is I don't have budget for new equipment right now. Jerry Ricks Research Scientist University of Washington Department of Pathology Effects of Fixative and Embedding Medium on Morphology and Immunostaining of the Cochlea Audiol Neurootol. 2009 ; 14(2): 78?87 > From: Barry.R.Rittman@uth.tmc.edu > To: histonet@lists.utsouthwestern.edu > Date: Mon, 20 Jul 2009 19:14:58 -0500 > Subject: RE: [Histonet] Celloidin Sections. > > Jerry > depends on whether you are talking about celloidin or Low Viscosity Nitrocelulose. > There are techniques in the literature to cut celloidin sections as thin as 4 microns. > For LVN you need much thicker sections as this plastic is much less sturdy and has a tendency to fragment if too thin. > The questions I ask is why you need celloidin sections and what tissue are you thinking about for this technique? > Barry > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R [rosenfeldtek@hotmail.com] > Sent: Monday, July 20, 2009 6:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Celloidin Sections. > > I am curious--How thin can celloidin sections be cut? I've heard that 30 microns is a standard thickness. No way to make 5 micron sections? > > Thanks, > > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Windows Live? SkyDrive?: Store, access, and share your photos. See how. > http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Bing? brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TXT_MLOGEN_Local_Local_Restaurants_1x1 From jkiernan <@t> uwo.ca Tue Jul 21 13:43:42 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jul 21 13:43:46 2009 Subject: [Histonet] A web site for tissue arrays and other sections on slides Message-ID: I recently came across the web site of a company that provides slides with sections of a wide variety of animal tissues - normal, pathological, transgenic etc: single and multiple tissues, as mounted paraffin sections. The associated photos indicate high quality fixation and sectioning. There are frequent Histonet enquiries about sources of negative and positive controls for immunohistochemistry, special stains etc. This might be such a source. The address is http://www.histobest.com John Kiernan Department of Anatomy & Cell Biology University of Western Ontario London, Canada. = = = From dunatrsd <@t> sbcglobal.net Tue Jul 21 14:00:12 2009 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Tue Jul 21 14:00:21 2009 Subject: [Histonet] MMP-2 antibodies In-Reply-To: <4A659E42020000D60004158F@mail.mrl.ubc.ca> References: <4A659E42020000D60004158F@mail.mrl.ubc.ca> Message-ID: <193591.40563.qm@web83902.mail.sp1.yahoo.com> I am also interested in MMP2 for staining human, as well as?mouse FFPE?tissue. Any information would be appreciated. Thanks Dusko ________________________________ From: Mark Elliott To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 21, 2009 10:53:54 AM Subject: [Histonet] MMP-2 antibodies I am looking for some advice as to which MMP-2 antibody people recommend for staining human tissue-either FFPE or frozen sections.? I have tried one from Novus as well as Serotec with no luck.? I looked in the Archives but couldn't find any.? Any suggestions greatly appreciated. Mark From jcampbell <@t> vdxpathology.com Tue Jul 21 14:30:24 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Tue Jul 21 14:30:30 2009 Subject: [Histonet] removing coverslip from immuno slides Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8261A56@VDXSERVER01.vdxpathology.local> I coverslipped some immuno slides using our Tissue-Tek coverslipper and I need to lift the coverslips from a few of the slides (used the wrong length). For histo slides, we typically soak the slides in acetone until the coverslipping film is easily removed. Is it ok to soak immuno slides in acetone to remove the film? Thank you, Jennifer From TJJ <@t> stowers.org Tue Jul 21 14:50:37 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Jul 21 14:51:30 2009 Subject: [Histonet] Re: double immunostaining with antigen retrieval and no antigen retrieval Message-ID: Cathy, You absolutely can do this, but you have to use a permanent, stable end product on the first antibody staining. This is easy to do if you use DAB as the first staining technique chromogen. The antigen retrieval will probably remove all antibody-antigen bonds (elution) from your first IHC reaction, so having a permanent label on the first one is critical. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From KGroeger <@t> USLABS.net Tue Jul 21 14:54:06 2009 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Tue Jul 21 14:54:12 2009 Subject: [Histonet] removing coverslip from immuno slides In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8261A56@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF8261A56@VDXSERVER01.vdxpathology.local> Message-ID: YES Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Tuesday, July 21, 2009 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] removing coverslip from immuno slides I coverslipped some immuno slides using our Tissue-Tek coverslipper and I need to lift the coverslips from a few of the slides (used the wrong length). For histo slides, we typically soak the slides in acetone until the coverslipping film is easily removed. Is it ok to soak immuno slides in acetone to remove the film? Thank you, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From rjbuesa <@t> yahoo.com Tue Jul 21 15:25:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 21 15:25:51 2009 Subject: [Histonet] removing coverslip from immuno slides Message-ID: <992638.51270.qm@web65712.mail.ac4.yahoo.com> Yes, you can use the same procedure. Ren? J. --- On Tue, 7/21/09, Jennifer Campbell wrote: From: Jennifer Campbell Subject: [Histonet] removing coverslip from immuno slides To: histonet@lists.utsouthwestern.edu Date: Tuesday, July 21, 2009, 3:30 PM ? I coverslipped some immuno slides using our Tissue-Tek coverslipper and I need to lift the coverslips from a few of the slides (used the wrong length).? For histo slides, we typically soak the slides in acetone until the coverslipping film is easily removed.? Is it ok to soak immuno slides in acetone to remove the film? Thank you, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Jul 21 15:33:36 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jul 21 15:33:41 2009 Subject: [Histonet] Fluorescent dyes and xylene Message-ID: Most of the Alexa Fluor dyes appear to be trade secrets: only a few are identified with chemical structures in the Molecular Probes/Invitrogen catalogue. Fluorescent labels of known identity do survive dehydration, clearing and mounting in a permanent medium and are still OK years later, stored in boxes at room temperature. It is, of course, necessary to use a non-fluorescent mountant such as DPX or one of the poly(methacrylate) ones (Entellan, Cytoseal etc). The notion that it's necessary to mount fluorescent preparations in slightly alkaline aqueous media may derive from an influential book by Nairn (1976), which stressed the effects of pH on fluorescence intensity and recommended mounting only in a somewhat alkaline glycerol-water mixture, and storage of slides in flat trays in a fridge. In fact, fluorescent labels bound to proteins survive dehydration, clearing and permanent mounting (see, eg, Stoddart 1973, Whyte 1978, Allen 1994, Espada 2005, Hertzler 2006). The paper by Espada et al (2005) illustrates this point very well and also discusses reasons why a polystyrene medium (ie DPX) may be best. You would not expect dehydration etc to remove or damage a fluorescently labelled antibody or other protein. Immersing the stained section in alcohol coagulates the protein instantly, along with the covalently attached fluorochrome molecules. References. Allen DT, Kiernan JA (1994) Permeation of proteins from the blood into peripheral nerves and ganglia. Neuroscience 99: 755-764. http://www.ncbi.nlm.nih.gov/pubmed/8008217?ordinalpos=33&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pacheco M, Stockert JC (2005) Non-aqueous permanent mounting for immunofluorescence microscopy. Histochem. Cell Biol. 123: 329-334. http://journals1.scholarsportal.info/details.xqy?uri=/09486143/v123i0003/329_npmfim.xml Hertzler PL (2006) Rhodamine fluorescence after 15-year storage in methyl salicylate. Microscopy Today 14-2: 48. http://www.microscopy-today.com/jsp/mto/print_archive/print_archive.faces Nairn RC (1976) Fluorescent Protein Tracing. 4th ed. Churchill-Livingstone, London. ISBN 0443012733. http://openlibrary.org/b/OL5063508M/Fluorescent-protein-tracing Stoddart RW, Kiernan JA (1973) Histochemical detection of the alpha-D-arabinopyranoside configuration using fluorescent-labelled concanavalin A. Histochemie 33: 87-94. http://www.springerlink.com/content/vk667214g6m80530/?p=fb8574a99977492fa339fb2300500e29&pi=2 Whyte A, Loke YW, Stoddart RW (1978) Saccharide distribution in human trophoblast demonstrated using fluorescein-labelled lectins. Histochem. J. 10: 417-423. http://www.springerlink.com/content/h710461877mk8010/ John Kiernan Department of Anatomy & Cell Biology University of Western Ontario London, Canada. = = = ----- Original Message ----- From: Bob Nienhuis Date: Monday, July 20, 2009 14:10 Subject: [Histonet] Fluorescent dyes and xylene To: Histonet > Will the Alexa Fluor dyes withstand some dehydration and > clearing in > alcohol and xylene? > > If they won't are there any bright fluorescent dyes that can be > obtainedcongugated to a secondary antibody that will? > > Looking to immunostain tyrosine hydroxylase in rodent brain with > a fluorescent marker in tissue that needs to be dehydrated and > cleared, perhaps with an abbreviated series. > > Bob Nienhuis > VA / UCLA Neurobiology Research > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thecitan <@t> yahoo.com Tue Jul 21 16:56:01 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Tue Jul 21 16:55:07 2009 Subject: [Histonet] Average pay rate southern cali Message-ID: <200844565-1248213303-cardhu_decombobulator_blackberry.rim.net-521854865-@bxe1123.bisx.prod.on.blackberry> I'm looking for an employee in southern california to be a lead tech for a derm lab. Just wondering what the histonet sees as far as pay rate for a tech with some experience. Please give me some hypothetical ballpark figures for a certified tech as well as uncertified, keeping in mind this person will do all routine work plus some administrative work. Thanks! Sent from my Verizon Wireless BlackBerry From estellamireles <@t> gmail.com Tue Jul 21 17:03:09 2009 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Jul 21 17:03:13 2009 Subject: [Histonet] Histology Job Opening - Houston Tx (suburb) Message-ID: We need a Histology Tech 3 - 5 yrs exp. to work days. M - F 6 - 2:30 pm General Duties - No Immuno's Heavy Bx's Small Lab. Looking for someone who takes pride in their work. Excellent cutting techniques and is dependable. Humble is located outside of Houston. Nice place to live. More Info call me: ( Please no agencies) Stella 281 540 6275 Apply at:. Memorial Hermann Hospital Northeast 18951 Memorial North Humble, Tx 77338 281 540 7700 From sheriblair1 <@t> netzero.net Tue Jul 21 20:47:51 2009 From: sheriblair1 <@t> netzero.net (sheriblair1@netzero.net) Date: Tue Jul 21 20:49:15 2009 Subject: [Histonet] Start up lab Message-ID: <20090721.204751.18254.0@webmail07.vgs.untd.com> A coverslipper would be nice. From andreas.kappeler <@t> pathology.unibe.ch Wed Jul 22 00:25:24 2009 From: andreas.kappeler <@t> pathology.unibe.ch (Kappeler Andi) Date: Wed Jul 22 00:25:36 2009 Subject: AW: [Histonet] MMP-2 antibodies In-Reply-To: <4A659E42020000D60004158F@mail.mrl.ubc.ca> References: <4A659E42020000D60004158F@mail.mrl.ubc.ca> Message-ID: <001b01ca0a8c$d0c5e5d0$17955c82@pi23> Hi Mark We were reasonably successful with mo-a-MMP-2, clone A-Gel VC2, from Thermo (NeoMarkers/Labvision). Working conc.: 2 ug/ml, pretreatment: HIER in TE, pH 9.0. Works on human and rat FFPE tissue, no experience with frozen sections. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Mark Elliott Gesendet: Dienstag, 21. Juli 2009 19:54 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] MMP-2 antibodies I am looking for some advice as to which MMP-2 antibody people recommend for staining human tissue-either FFPE or frozen sections. I have tried one from Novus as well as Serotec with no luck. I looked in the Archives but couldn't find any. Any suggestions greatly appreciated. Mark From Lea.Alminde <@t> tuhs.temple.edu Wed Jul 22 07:53:04 2009 From: Lea.Alminde <@t> tuhs.temple.edu (Alminde, Lea S) Date: Wed Jul 22 07:53:16 2009 Subject: [Histonet] Histology Job Opening Message-ID: To All We have a Histology Job Opening Day Shift 730a to 4 pm. Please call or send resume. Thanks Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email almindls@tuhs.temple.edu ________________________________ This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. Direct Questions to the Corporate Compliance and Privacy Officer at (215) 707-5605. From Lea.Alminde <@t> tuhs.temple.edu Wed Jul 22 07:53:04 2009 From: Lea.Alminde <@t> tuhs.temple.edu (Alminde, Lea S) Date: Wed Jul 22 07:55:12 2009 Subject: [Histonet] Histology Job Opening Message-ID: To All We have a Histology Job Opening Day Shift 730a to 4 pm. Please call or send resume. Thanks Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email almindls@tuhs.temple.edu ________________________________ This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. Direct Questions to the Corporate Compliance and Privacy Officer at (215) 707-5605. From kmerriam2003 <@t> yahoo.com Wed Jul 22 08:57:53 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jul 22 08:57:58 2009 Subject: [Histonet] histology for kids Message-ID: <930270.46819.qm@web50303.mail.re2.yahoo.com> Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From dchihc <@t> yahoo.com Wed Jul 22 09:04:23 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Jul 22 09:04:27 2009 Subject: [Histonet] Formula 83 Message-ID: <685228.33131.qm@web43507.mail.sp1.yahoo.com> Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The?label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From lblazek <@t> digestivespecialists.com Wed Jul 22 09:15:15 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 22 09:12:55 2009 Subject: [Histonet] Formula 83 In-Reply-To: <685228.33131.qm@web43507.mail.sp1.yahoo.com> References: <685228.33131.qm@web43507.mail.sp1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088E58A1F7@IBMB7Exchange.digestivespecialists.com> Phyllis, I have used Formula 83 for several years and have no problem with it. I do use it just like xylene. As for how frequently you need to change it on the processor really depends on your volume of processing. I didn't have to change the frequency when I switched from xylene to Formula 83. I also recycle my Formula 83. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Wednesday, July 22, 2009 10:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83 Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The?label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 22 09:15:30 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 22 09:15:34 2009 Subject: [Histonet] histology for kids Message-ID: <14687.78587.qm@web65713.mail.ac4.yahoo.com> Try saffron, in reality it is an acceptable stain for "regular grown-up histology" as well. Ren? J. --- On Wed, 7/22/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] histology for kids To: "Histonet" Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Breckenridge <@t> uth.tmc.edu Wed Jul 22 09:32:09 2009 From: Richard.Breckenridge <@t> uth.tmc.edu (Breckenridge, Richard A) Date: Wed Jul 22 09:32:16 2009 Subject: [Histonet] Histotech II position open. Message-ID: <3FE6D14CF4B4174AA4783A50F58CD30D0413686437@UTHCMS3.uthouston.edu> Hello all. I would like to inform the histotech community of an opening for a Histotech II position. We are looking for someone with experience who is willing to learn. Renal, Muscle, and IHC experience is a definite plus, but we are willing to teach if the candidate is motivated. Please apply at www.uthouston.edu and follow the careers link to the allied health category. Thanks! Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu From BoozerKA <@t> ah.org Wed Jul 22 09:46:59 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Wed Jul 22 09:47:21 2009 Subject: [Histonet] histology for kids In-Reply-To: <14687.78587.qm@web65713.mail.ac4.yahoo.com> References: <14687.78587.qm@web65713.mail.ac4.yahoo.com> Message-ID: <4A66C3F2.4AA8.00C0.0@ah.org> Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. >>> Rene J Buesa 07/22/2009 07:15 >>> Try saffron, in reality it is an acceptable stain for "regular grown-up histology" as well. Ren? J. --- On Wed, 7/22/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] histology for kids To: "Histonet" Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed Jul 22 09:55:00 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Jul 22 09:55:07 2009 Subject: [Histonet] histology for kids In-Reply-To: <4A66C3F2.4AA8.00C0.0@ah.org> References: <14687.78587.qm@web65713.mail.ac4.yahoo.com> <4A66C3F2.4AA8.00C0.0@ah.org> Message-ID: red food colouring, green and blue too as well as beetroot and grape juice and saffron could be tried in advance on the slides - just to see how colours can be combined you will only know by testing in advance - or you may end up with a brown sludge covering the whole section look at hairs and fleas and tiny bugs under the 'scope - they always intrigue kids skin scales on a slide stained with 3 diff kwik solutions or a PAP stain would work too just my 5cents worth Anne 2009/7/22 Kathleen Boozer > Maybe you could use a sponge (representing tissue) soaked in water and > demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled > (precise cutting) explaining the water is taken out of the cells and > replaced with wax. > > >>> Rene J Buesa 07/22/2009 07:15 >>> > Try saffron, in reality it is an acceptable stain for "regular grown-up > histology" as well. > Ren? J. > > --- On Wed, 7/22/09, Kim Merriam wrote: > > > From: Kim Merriam > Subject: [Histonet] histology for kids > To: "Histonet" > Date: Wednesday, July 22, 2009, 9:57 AM > > > Hello All, > > My company is hosting an in-house science awareness day for local > grade-school students. I would love to teach them about histology, but > all of the demonstrations need to be done in our conference room (thus, > nothing hazardous). Does anyone know of any house-hold dyes (grape > juice, food coloring, beet juice, etc) that would stain tissue elements > on slides? I would like to bring down some deparaffinized tissues and > stain them with something and throw a coverslip on (water-mounted) so > that they can look at the tissue with a microscope. I will also bring > some already prepared slides (wtih real stains) for them to look at. > > Any ideas? > > Thanks, > Kim > > > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From lamcna <@t> bpthosp.org Wed Jul 22 09:58:19 2009 From: lamcna <@t> bpthosp.org (McNabola, Angela) Date: Wed Jul 22 09:58:27 2009 Subject: [Histonet] histology for kids In-Reply-To: <4A66C3F2.4AA8.00C0.0@ah.org> Message-ID: <6FAFE015E5AC6B4ABAEF2883A6F8A6D902336437@EXCH1.bpthosp.org> I recently taught at my daughter's first grade. I focused less on the histology, and more on the mircroscope, cells etc. I did bring in some prepared slides and talked a little about how I got to that point on an elementary level. I then let them make their own wet mount slide of choice to look at under the scope. The teacher had them draw what they saw in a "journal" I had prepared for them.. Basically a few sheets of paper. Their choice of slide was : -a swab of their cheek that we stained with c violet -yougurt -small piece of celery -dust "bunnies" from under my daughter's bed -dog hair -lint from the dryer It was a great hit. If you google microscope and kids, the options are endless. Hope that this helps........ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Wednesday, July 22, 2009 10:47 AM To: Histonet; Kim Merriam; Rene J Buesa Subject: Re: [Histonet] histology for kids Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. >>> Rene J Buesa 07/22/2009 07:15 >>> Try saffron, in reality it is an acceptable stain for "regular grown-up histology" as well. Ren? J. --- On Wed, 7/22/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] histology for kids To: "Histonet" Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Wed Jul 22 10:09:27 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Jul 22 10:09:31 2009 Subject: [Histonet] Peloris tissue processor Message-ID: <37A1F9CAB9E21C41B39F3653B620D13E093BA543@exchange2003.lc.local> Anyone currently using the Peloris tissue processor please comment- love it, likes, dislikes? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com From ploykasek <@t> phenopath.com Wed Jul 22 10:12:25 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jul 22 10:12:34 2009 Subject: [Histonet] Slide dryers Message-ID: Hi All. We are re-working some of our processes (fun!). We are trying to get more LEAN and shave some time off of our processes here & there. One thing I am looking at is the small forced air slide dryers. I have seen some good comments on the mopec roto-dry. Has anyone tried the dryer from EMS? What are people using and liking? We will be doing IHC on these sections. What type of drying time are you using? I really appreciate the info. We are having a series of quick meetings, and I am having to gather information quickly. Thanks for the help. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From rsrichmond <@t> gmail.com Wed Jul 22 10:16:16 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jul 22 10:16:23 2009 Subject: [Histonet] Re: Formula 83 Message-ID: We discussed formula 83 earlier this year. http://lists.utsouthwestern.edu/pipermail/histonet/2009-January/041976.html Bob Richmond Samurai Pathologist Knoxville TN From relia1 <@t> earthlink.net Wed Jul 22 10:20:46 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jul 22 10:20:53 2009 Subject: [Histonet] Histology Supervisor needed in Washington State Message-ID: Hello Histonetters! I have a new position I want to tell you about. I am currently working with a hospital located in Washington State who is in need of a histology supervisor. ASCP HT, Hands -on histology and supervisory experience is required. My client offers a great salary and benefits and relocation assistance. If you or anyone you know might be interested please contact me at 866-607-3542 or relia1@earthlink.net. Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Timothy.Morken <@t> ucsfmedctr.org Wed Jul 22 10:21:37 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Jul 22 10:22:51 2009 Subject: [Histonet] histology for kids In-Reply-To: <930270.46819.qm@web50303.mail.re2.yahoo.com> References: <930270.46819.qm@web50303.mail.re2.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C680CCD60@EXMBMCB15.ucsfmedicalcenter.org> Try this website for ideas... http://www.mnmicroscopy.org/ProjectMicro/Welcome.html Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 6:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Wed Jul 22 10:29:17 2009 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Jul 22 10:29:21 2009 Subject: [Histonet] Start Up Lab Message-ID: <5d9104a30907220829v525cdc7ftcab910d061ee72d4@mail.gmail.com> And a coffee pot. From jmjohnson34 <@t> hotmail.com Wed Jul 22 10:33:19 2009 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Wed Jul 22 10:33:24 2009 Subject: [Histonet] Histology for kids Message-ID: I did tonsils one time and since most of them have had theirs removed, they were very interested. We also have autopsy tissue, brains, lung, liver, heart, in jars of formalin which are always a hit for them to see but not touch! Good Luck, it will be so much fun watching their faces. Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Windows Live? Hotmail?: Search, add, and share the web?s latest sports videos. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGLM_WL_QA_HM_sports_videos_072009&cat=sports From MSHERWOOD <@t> PARTNERS.ORG Wed Jul 22 11:01:08 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jul 22 11:01:15 2009 Subject: [Histonet] histology for kids In-Reply-To: <930270.46819.qm@web50303.mail.re2.yahoo.com> References: <930270.46819.qm@web50303.mail.re2.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23B6D@PHSXMB30.partners.org> Kim, Contact Mary McCann who is ProjectMICRO's coordinator for NESM (New England Society for Microscopy). NESM took on ProjectMICRO as a "pet project" a number of years back. We put together 3 kits of materials (microscopes and consumables). Two of the kits are in constant use in Vermont and Maine. Mary is in charge of the 3rd kit around this area. She also comes in and does "festivals" which consist of different exploratory stations. Go to NESM's website: http://nesm.cims.harvard.edu/ and click on ProjectMICRO. Mary's contact info is there. I have done several of these festivals and also worked at the Cambridge Science Day. The kids love these exercises! Good luck and have fun! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 9:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From PMonfils <@t> Lifespan.org Wed Jul 22 11:09:30 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 22 11:09:34 2009 Subject: [Histonet] histology for kids In-Reply-To: <930270.46819.qm@web50303.mail.re2.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CCF@LSRIEXCH1.lsmaster.lifespan.org> A number of dyes used in histology are also approved for use in foods. These include: Brilliant Blue (FD&C Blue #1) Fast Green FCF (FD&C Green #3) Erythrosin (FD&C Red #3) Tartrazine (FD&C Yellow #5) Carmine From gentras <@t> vetmed.auburn.edu Wed Jul 22 11:32:03 2009 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jul 22 11:32:09 2009 Subject: [Histonet] Re: Citadel 2000 Tissue Processor Message-ID: <4A673F03.9090108@vetmed.auburn.edu> Hello, will everyone who's used/using the Citadel 2000 Tissue Processor please share with me your opinions, pros/cons and etc ASAP? Thanks! Atoska From asmith <@t> mail.barry.edu Wed Jul 22 11:29:22 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jul 22 11:32:13 2009 Subject: [Histonet] histology for kids In-Reply-To: <930270.46819.qm@web50303.mail.re2.yahoo.com> References: <930270.46819.qm@web50303.mail.re2.yahoo.com> Message-ID: If you ripen it with air or sodium iodate, alum hematoxylin is quite safe. FD&C green #3 is food grade fast green FCF, an excellent stain for collagen. FD&C yellow #5 is tartrazine, a plasma stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 9:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BoozerKA <@t> ah.org Wed Jul 22 11:41:29 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Wed Jul 22 11:41:56 2009 Subject: [Histonet] histology for kids In-Reply-To: <4A66C3F2.4AA8.00C0.0@ah.org> References: <14687.78587.qm@web65713.mail.ac4.yahoo.com> <4A66C3F2.4AA8.00C0.0@ah.org> Message-ID: <4A66DEC8.4AA8.00C0.0@ah.org> One more thought, NSH has a small paper pamphlet out call Histology (hiss TOL-o-je) which has pictures, puzzles, anatomy charts (simple) to help teach kids. Written by Judy Stasko, CLT and Jan Gardiner, BAAS, HT(ASCP). >>> "Kathleen Boozer" 07/22/2009 07:46 >>> Maybe you could use a sponge (representing tissue) soaked in water and demonstrate cutting (ragged) vs. a sponge soaked in wax and cooled (precise cutting) explaining the water is taken out of the cells and replaced with wax. >>> Rene J Buesa 07/22/2009 07:15 >>> Try saffron, in reality it is an acceptable stain for "regular grown-up histology" as well. Ren? J. --- On Wed, 7/22/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] histology for kids To: "Histonet" Date: Wednesday, July 22, 2009, 9:57 AM Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Wed Jul 22 11:42:24 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Wed Jul 22 11:42:51 2009 Subject: [Histonet] Chromium Trioxide Message-ID: <4F0B7161A6CD524FAD8017D52E1553400A768A6C@exchangent> Fellow Histonetters, We use Chromium Trioxide in our GMS stain, and the cost has escalated tremendously. Does anyone use a substitute for this chemical? I am curious what others are using for their GMS stain. We still do our stains manually at the present time. Thank you for your advice. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From arvidsonkristen <@t> yahoo.com Wed Jul 22 11:58:05 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Jul 22 11:58:09 2009 Subject: [Histonet] More quality stuff Message-ID: <293239.69035.qm@web65707.mail.ac4.yahoo.com> Hi- As most of you have gathered I am working on a large quality project.? I have made a lot of progress. ?Currently, my focus is error prevention.? I have read up on tools such as six sigma and FMEA.? My question is how do you guys prevent errors?? I have learned that you cannot always count on the diligence of the employee because even the best will screw up sooner or later, so my focus is on process improvement.? How are you all approaching your process improvements?? ? Thank you all for your input.? You've all been a big help!! ? -Kristen From BoozerKA <@t> ah.org Wed Jul 22 12:26:14 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Wed Jul 22 12:27:09 2009 Subject: [Histonet] More quality stuff In-Reply-To: <293239.69035.qm@web65707.mail.ac4.yahoo.com> References: <293239.69035.qm@web65707.mail.ac4.yahoo.com> Message-ID: <4A66E945.4AA8.00C0.0@ah.org> We have had tremendous success with setting up "checkpoints" at each step in tissue processing (cradle to grave). Each checkpoint has certain responsibilities to identify, double-check, and assure accuracy. I keep track of discrepancy errors at each checkpoint and determine if personnel need to be re-trained, held accountable, or possibly add or revise checkpoints. It takes a lot of time to do this. What I have found though, is that we prevent most errors by the checkpoint after it initially happens and long before it goes out of the department. Kathy >>> kristen arvidson 07/22/2009 09:58 >>> Hi- As most of you have gathered I am working on a large quality project. I have made a lot of progress. Currently, my focus is error prevention. I have read up on tools such as six sigma and FMEA. My question is how do you guys prevent errors? I have learned that you cannot always count on the diligence of the employee because even the best will screw up sooner or later, so my focus is on process improvement. How are you all approaching your process improvements? Thank you all for your input. You've all been a big help!! -Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Wed Jul 22 12:29:38 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jul 22 12:29:51 2009 Subject: [Histonet] More quality stuff Message-ID: <4A671442020000EE0002AEF6@smtp-gw.hurleymc.com> Hi Kathy, Do you have a sample form you would be willing to share? thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Kathleen Boozer" 07/22/09 1:26 PM >>> We have had tremendous success with setting up "checkpoints" at each step in tissue processing (cradle to grave). Each checkpoint has certain responsibilities to identify, double-check, and assure accuracy. I keep track of discrepancy errors at each checkpoint and determine if personnel need to be re-trained, held accountable, or possibly add or revise checkpoints. It takes a lot of time to do this. What I have found though, is that we prevent most errors by the checkpoint after it initially happens and long before it goes out of the department. Kathy >>> kristen arvidson 07/22/2009 09:58 >>> Hi- As most of you have gathered I am working on a large quality project. I have made a lot of progress. Currently, my focus is error prevention. I have read up on tools such as six sigma and FMEA. My question is how do you guys prevent errors? I have learned that you cannot always count on the diligence of the employee because even the best will screw up sooner or later, so my focus is on process improvement. How are you all approaching your process improvements? Thank you all for your input. You've all been a big help!! -Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Jul 22 13:16:17 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jul 22 13:16:21 2009 Subject: [Histonet] histology for kids In-Reply-To: <930270.46819.qm@web50303.mail.re2.yahoo.com> References: <930270.46819.qm@web50303.mail.re2.yahoo.com> Message-ID: <367227.65989.qm@web50306.mail.re2.yahoo.com> Thanks?to everyone that emailed me, I received so many ideas!? I will let you all know?what I end up donig. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Kim Merriam To: Histonet Sent: Wednesday, July 22, 2009 9:57:53 AM Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed Jul 22 13:22:53 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Jul 22 13:22:59 2009 Subject: [Histonet] Start Up Lab Message-ID: I would add an eye wash station and a chemical spill kit to the list. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From rsrichmond <@t> gmail.com Wed Jul 22 14:02:01 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jul 22 14:02:05 2009 Subject: [Histonet] Re: Chromium Trioxide Message-ID: Deanne Knutson, Anatomic Pathology Supervisor, St. Alexius Medical Center, Bismarck, North Dakota asks: >>We use Chromium Trioxide in our GMS stain, and the cost has escalated tremendously. Does anyone use a substitute for this chemical? I am curious what others are using for their GMS stain. We still do our stains manually at the present time.<< There is no completely satisfactory substitute for chromium trioxide (chromic acid) for the oxidation step for the GMS stain for fungi, particularly if you're trying to stain Histoplasma. Many kits substitute periodic acid, usually in inadequate amounts. Freida Carson published a careful study of this problem several years ago and concluded that periodic acid could be substituted, with sufficient time and temperature. (I think I can find this reference, but it's probably already in our archives.) Last time I looked (2006) the Ventana method still used chromium trioxide. Bob Richmond Samurai Pathologist Knoxville TN From freidac <@t> sbcglobal.net Wed Jul 22 15:05:28 2009 From: freidac <@t> sbcglobal.net (Freida Carson) Date: Wed Jul 22 15:05:33 2009 Subject: [Histonet] Re: Chromium Trioxide Message-ID: <360344.7445.qm@web82505.mail.mud.yahoo.com> Bob is correct that there is no satisfactory substitute for chromic acid in the GMS procedure.? We found that it takes 1 hour at 56-60 degrees C in 1% periodic acid to equal the usual oxidation with chromic acid, and even then you will probable get more silver staining of the connective tissue than with chromic acid.? The paper was published in the J Histotechnol, 1999; Vol 22:119. ? If you rinse sections very well with water before the chromic acid, the chromic acid can be used for over and over.? It is alcohol remaining on the slides that causes the chromic acid to turn brown and become unusuable. ? Hope this helps. ? Freida Carson --- On Wed, 7/22/09, Robert Richmond wrote: From: Robert Richmond Subject: [Histonet] Re: Chromium Trioxide To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 22, 2009, 2:02 PM Deanne Knutson, Anatomic Pathology Supervisor, St. Alexius Medical Center, Bismarck, North Dakota asks: >>We use Chromium Trioxide in our GMS stain, and the cost has escalated tremendously. Does anyone use a substitute for this chemical?? I am curious what others are using for their GMS stain. We still do our stains manually at the present time.<< There is no completely satisfactory substitute for chromium trioxide (chromic acid) for the oxidation step for the GMS stain for fungi, particularly if you're trying to stain Histoplasma. Many kits substitute periodic acid, usually in inadequate amounts. Freida Carson published a careful study of this problem several years ago and concluded that periodic acid could be substituted, with sufficient time and temperature. (I think I can find this reference, but it's probably already in our archives.) Last time I looked (2006) the Ventana method still used chromium trioxide. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Jul 22 15:19:55 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jul 22 15:20:32 2009 Subject: [Histonet] Start Up Lab References: <5d9104a30907220829v525cdc7ftcab910d061ee72d4@mail.gmail.com> Message-ID: In the lab?!? For shame. :) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Jul 22 16:00:01 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Jul 22 16:00:07 2009 Subject: [Histonet] Start Up Lab In-Reply-To: References: <5d9104a30907220829v525cdc7ftcab910d061ee72d4@mail.gmail.com> Message-ID: <4C8551932D7D97C905FE204F@CDYwxp1931.ad.med.buffalo.edu> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From macveigh <@t> usc.edu Wed Jul 22 16:17:09 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Jul 22 16:17:04 2009 Subject: [Histonet] Re: Citadel Tissue Processor Message-ID: <004601ca0b11$c5dd88a0$5c237d80@DFS66DD1> Hi Atoska, We do only research and have low volume. We purchased a second hand one and used it for about a year. It works and does the job. It is much better than having to process manually but: The alcohols evaporate like crazy! You loose a lot. It must be in a hood. There is no heating nor vacuum. I was under the impression that there is vacuum while reading the brochure, but when the Citadel arrived, I found that it is a separate free standing unit that can be connected only with the paraffin tanks. We didn't have the room for it so we never used it and sent it back. There is no purge/clean cycle. You have to either purchase 2 extra buckets or find some other containers for the Xylene and the 100% which you will be using to manually wash the basket after the cassettes are out of it. They should also be in the hood. In a year, one of the paraffin buckets burned. The service guy told me that a repair was not worth it. A new paraffin bath is about $1200.00. Something that was good is that you only need about 1/2 gallon per station. When we had only few cassettes, we didn't even fill completely the buckets. Infiltration was fine. Hope this helps Michelle USC Keck School of Medicine From lpaveli1 <@t> hurleymc.com Wed Jul 22 17:07:07 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jul 22 17:07:28 2009 Subject: [Histonet] Start Up Lab Message-ID: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thecitan <@t> yahoo.com Wed Jul 22 17:59:24 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Wed Jul 22 17:58:29 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> Message-ID: <210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Jul 22 18:03:48 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 22 18:01:26 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088E58A1FF@IBMB7Exchange.digestivespecialists.com> Hay! Another one of us "oldies"! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, July 22, 2009 6:07 PM To: leiker@buffalo.edu; histonet@lists.utsouthwestern.edu; CIngles@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbowden <@t> ucsd.edu Wed Jul 22 18:46:31 2009 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed Jul 22 18:46:36 2009 Subject: [Histonet] 100 micron sections Message-ID: <4A67A4D7.1040107@ucsd.edu> Hi, I am being asked to section paraffin embedded fat at 100 microns. I haven't sectioned anything in paraffin thicker than 20 microns. What are the tricks to get it to unroll in the water bath? -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER./* From ccrowder <@t> vetmed.lsu.edu Wed Jul 22 23:37:22 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Jul 22 23:39:13 2009 Subject: [Histonet] Chromium trioxide Message-ID: You can use periodic acid to oxidize your tissues as you would for the PAMS stain. However, this is not the optimal solution when staining fungi. 5% chromium trioxide used for the GMS can be reused. Since you are doing the stains manually, as many of us do, this works particularly well. The fresh chromium trioxide is a clear orange color. It can be reused until the color turns more brown in color. This indicates that the oxidizing potential has been depleted. You can also use 3% chromium trioxide instead of the 5% and heat the solution to 55 degrees and oxidize for 30 minutes. Finally you can use 2% chromic acid in the microwave oven at 70% power for 45 seconds (solution should be about 55 degrees). By using milder solutions or reusing your solution you will save money and time. Cheryl From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jul 23 03:16:27 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jul 23 03:16:33 2009 Subject: [Histonet] histology for kids Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0720B160@wahtntex2.waht.swest.nhs.uk> Depends on the age of the kids as I don't understand the term 'grade-school'. What I did for kids around 10 yesrs old or so was to go to the Butchers and get some Ox kidney, heart and liver. I prepared slides from them, took a microscope to let them see the structure and also took scapels for them to cut up the animal tissue. Odd how many kids haven't handled animal organs or raw meat. Anyways be careful of the scapel maybe you risk adverse Americans ought just to use scissors or a pen knife. Ask the kids they might be carrying a blade!! (joke, joke, honest). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: 22 July 2009 14:58 To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students.? I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous).? Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides?? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope.? I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly.Marshall <@t> ahss.org Thu Jul 23 07:19:12 2009 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Thu Jul 23 07:20:07 2009 Subject: [Histonet] Frozen H&E Message-ID: Hello Histo folks I am having a hard time with consistency with my frozen H&E. It was always to the Pathologist liking till the last year. I have not changed stains or reagents so I am not sure what may be the problem. Would anyone care to share with me their frozen section H&E procedure? Maybe I just need to change the whole process. I do filter and change the stains weekly and reagents as needed, so I am not sure what else to try. Thanks in advance for the help Kimberly Marshall HT (ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From kmerriam2003 <@t> yahoo.com Thu Jul 23 07:51:59 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jul 23 07:52:03 2009 Subject: [Histonet] who owns microm these days? Message-ID: <165160.26831.qm@web50308.mail.re2.yahoo.com> is it ThermoFisher? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Carol.Wilson <@t> ricerca.com Thu Jul 23 08:15:31 2009 From: Carol.Wilson <@t> ricerca.com (Wilson, Carol) Date: Thu Jul 23 08:15:36 2009 Subject: [Histonet] Cyclin D3 Message-ID: <9D443EB9D0270143B5AAF190CB1A58A309746F29@dogwood.ricerca.com> Hello All, Is anyone out there currently doing Cyclin D3? We are having trouble getting any staining at all. We are doing manual heat retrieval and staining. Any suggestions or protocols would be appreciated. Thanks in advance, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology From leiker <@t> buffalo.edu Thu Jul 23 08:41:40 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Jul 23 08:41:43 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39088E58A1FF@IBMB7Exchange.digestivespecialists.com> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> <5A2BD13465E061429D6455C8D6B40E39088E58A1FF@IBMB7Exchange.digestivespecialists.com> Message-ID: <28C79AA8A62FE5F73DFE4706@CDYwxp1931.ad.med.buffalo.edu> well...maybe it's just research labs at UB then...i guess we're pretty "old" school! --On Wednesday, July 22, 2009 7:03 PM -0400 "Blazek, Linda" wrote: > Hay! Another one of us "oldies"! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette > Pavelich Sent: Wednesday, July 22, 2009 6:07 PM > To: leiker@buffalo.edu; histonet@lists.utsouthwestern.edu; > CIngles@uwhealth.org Subject: RE: [Histonet] Start Up Lab > > Gosh.........I remember the days sipping on my coffee and nibbling on a > fresh donut as I cut my morning slides! Sigh...... > >>>> Merced M Leiker 07/22/09 5:00 PM >>> > (lol some labs have a bench area as well as a desk area where food is > allowed.) > > --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire > wrote: > >> In the lab?!? For shame. :) >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy > DuBois >> Sent: Wed 7/22/2009 10:29 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Start Up Lab >> >> >> >> And a coffee pot. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From cbrya <@t> lexclin.com Thu Jul 23 09:03:57 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Jul 23 09:04:02 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com> <210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Thu Jul 23 10:32:34 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Thu Jul 23 10:39:36 2009 Subject: [Histonet] who owns microm these days? In-Reply-To: <165160.26831.qm@web50308.mail.re2.yahoo.com> References: <165160.26831.qm@web50308.mail.re2.yahoo.com> Message-ID: <4FE7FB862E90E448AE32388E759220E5016CD7FF@pbrcas31.pbrc.edu> Yes, ThermoFisher Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, July 23, 2009 7:52 AM To: Histonet Subject: [Histonet] who owns microm these days? is it ThermoFisher? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Thu Jul 23 10:31:29 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Thu Jul 23 10:39:40 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com><210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> Message-ID: <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Jul 23 10:44:28 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jul 23 10:45:45 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com><210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD33FE@LTA3VS011.ees.hhs.gov> And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Jul 23 10:51:05 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Jul 23 10:51:25 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE101AD33FE@LTA3VS011.ees.hhs.gov> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com><210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> <9A16CB5D55FC1648ADF11B63E72A1BE101AD33FE@LTA3VS011.ees.hhs.gov> Message-ID: <7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D@EXC-MBX3.cfs.le.ac.uk> You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Thu Jul 23 10:51:16 2009 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Thu Jul 23 10:51:28 2009 Subject: [Histonet] Rat's lung Message-ID: <63947.202.125.145.178.1248364276.squirrel@brain.net.pk> Dear All, How the volume of Rat's lung can be meausured? Thanks advance Muhammad Tahseen Histology Supervisor SKMCH&RC lAHORE,pAKISTAN From lpaveli1 <@t> hurleymc.com Thu Jul 23 10:53:27 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Jul 23 10:53:45 2009 Subject: [Histonet] Start Up Lab Message-ID: <4A684F37020000EE0002AFD3@smtp-gw.hurleymc.com> Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) >>> "Montina Van Meter" 07/23/09 11:31 AM >>> I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Jul 23 11:13:19 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jul 23 11:13:32 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D@EXC-MBX3.cfs.le.ac.uk> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com><210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> <9A16CB5D55FC1648ADF11B63E72A1BE101AD33FE@LTA3VS011.ees.hhs.gov> <7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D@EXC-MBX3.cfs.le.ac.uk> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3400@LTA3VS011.ees.hhs.gov> Hah! Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Thu Jul 23 11:26:26 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jul 23 11:29:13 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3400@LTA3VS011.ees.hhs.gov> References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com><210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> <9A16CB5D55FC1648ADF11B63E72A1BE101AD33FE@LTA3VS011.ees.hhs.gov> <7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D@EXC-MBX3.cfs.le.ac.uk> <9A16CB5D55FC1648ADF11B63E72A1BE101AD3400@LTA3VS011.ees.hhs.gov> Message-ID: I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Jul 23 11:32:07 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jul 23 11:32:49 2009 Subject: [Histonet] Start Up Lab In-Reply-To: References: <4A67554B020000EE0002AF33@smtp-gw.hurleymc.com><210121995-1248303504-cardhu_decombobulator_blackberry.rim.net-1447021100-@bxe1123.bisx.prod.on.blackberry> <50DA0C6B72976B4AB3A0FCA04CC73DBF172B3829@EXCHANGESB> <4FE7FB862E90E448AE32388E759220E5016CD7FE@pbrcas31.pbrc.edu> <9A16CB5D55FC1648ADF11B63E72A1BE101AD33FE@LTA3VS011.ees.hhs.gov> <7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D@EXC-MBX3.cfs.le.ac.uk> <9A16CB5D55FC1648ADF11B63E72A1BE101AD3400@LTA3VS011.ees.hhs.gov> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3402@LTA3VS011.ees.hhs.gov> Thanks to spell-check......it self-corrected me......incorrectly! Thanks for setting it right. -----Original Message----- From: Smith, Allen [mailto:asmith@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Jul 23 11:47:52 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Jul 23 11:48:00 2009 Subject: [Histonet] Peloris tissue processor In-Reply-To: <37A1F9CAB9E21C41B39F3653B620D13E093BA543@exchange2003.lc.local> Message-ID: <65281717443C4C1385063D050EB7A387@wchsys.org> We have had our Peloris since Feb. and we love it. We use the 6 hour program for our regular tissue and the 8 hour program for breast, thyroid, colon, melanoma, sentinel nodes, prostatectomy. The one hour program for prostate biopsies. We use Formula 83 instead of Xylene, or you can use Xylene free processing using Isopropyl alcohol. We like being able to run both retorts at the same time, of course they don't finish at the same time but closely. Your reagent usage drops also. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, July 22, 2009 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Anyone currently using the Peloris tissue processor please comment- love it, likes, dislikes? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From vapatpxs <@t> yahoo.com Thu Jul 23 12:30:33 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Jul 23 12:30:55 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <4A684F37020000EE0002AFD3@smtp-gw.hurleymc.com> Message-ID: <532021.17532.qm@web46102.mail.sp1.yahoo.com> In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl).? Several of the techs got nose bleeds every time they used the stuff.? I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window. We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause "transient euphoria".? No wonder we thought every thing was funny during those days. Safety people, they take all the fun out of research!? ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: RE: [Histonet] Start Up Lab To: cbrya@lexclin.com, Montina.VanMeter@pbrc.edu, thecitan@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 3:53 PM Ah, yes.? The '70's.? Our lab had 2 windows, both on the same side of the wall.? The pathologists cut under one of the windows and it had one of those box fans, pointing out.? In Michigan.? No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared.? Ah..those were the days??? Although I miss it, I'm glad I had to give up my coffee and donut when cutting.? Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!)? Progress is good.? But I still like my '70's music!!!? Bob Seger and old motown!!! ;) >>> "Montina Van Meter" 07/23/09 11:31 AM >>> I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on? cigarettes with an ashtray perched on top of her microtome.? When we had inspections everyone would put their contraband in their "personal drawer".? I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood.? That supervisor also smoked in the lab!? I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay.? Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA? 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it!? Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides!? Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs <@t> yahoo.com Thu Jul 23 12:31:36 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Jul 23 12:31:47 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3402@LTA3VS011.ees.hhs.gov> Message-ID: <335343.28523.qm@web46105.mail.sp1.yahoo.com> You might still have your fingers, but are your fingerprints still the same?? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: From: Bartlett, Jeanine (CDC/CCID/NCZVED) Subject: RE: [Histonet] Start Up Lab To: "Smith, Allen" Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 4:32 PM Thanks to spell-check......it self-corrected me......incorrectly! Thanks for setting it right. -----Original Message----- From: Smith, Allen [mailto:asmith@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent.? I think you mean dioxane. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah!? Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still? have? fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on? cigarettes with an ashtray perched on top of her microtome.? When we had inspections everyone would put their contraband in their "personal drawer".? I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood.? That supervisor also smoked in the lab!? I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay.? Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA? 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it!? Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides!? Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214? USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbowden <@t> ucsd.edu Thu Jul 23 12:40:57 2009 From: kbowden <@t> ucsd.edu (kbowden) Date: Thu Jul 23 12:41:14 2009 Subject: [Histonet] 100 micron sections In-Reply-To: <94194.10792.qm@web63705.mail.re1.yahoo.com> References: <4A67A4D7.1040107@ucsd.edu> <94194.10792.qm@web63705.mail.re1.yahoo.com> Message-ID: <4A68A0A9.6080302@ucsd.edu> Yes, with H&E staining. They think they need a thick section because they are looking for product that is 75mm round. I don't seem to be able to make them understand that it is not necessary to cut that thick to see the beads they are looking for. */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER./* Barbara Albert wrote: > Karen, > Are you certain that they want these sections on slides? The thicker > ones we usually put into a microcentrifuge tube. No water bath, and > the curled section fits into the tube. > > Barbara Albert > San Francisco > > ------------------------------------------------------------------------ > *From:* kbowden > *Cc:* histonet > *Sent:* Wednesday, July 22, 2009 4:46:31 PM > *Subject:* [Histonet] 100 micron sections > > Hi, > > I am being asked to section paraffin embedded fat at 100 microns. I > haven't sectioned anything in paraffin thicker than 20 microns. What > are the tricks to get it to unroll in the water bath? -- */-- > Karen Bowden > Staff Research Associate II > University of CA, San Diego > Department of Orthopedics > 9500 Gilman Dr. 0630 > La Jolla, CA 92093-0630 > 858-534-4655 voice > 858-534-5304 fax > > > CONFIDENTIALITY NOTICE: > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. > IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND > DELETE THE MATERIAL FROM ANY COMPUTER./* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tpodawiltz <@t> lrgh.org Thu Jul 23 12:40:43 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Jul 23 12:43:20 2009 Subject: [Histonet] Start Up Lab In-Reply-To: <532021.17532.qm@web46102.mail.sp1.yahoo.com> References: <4A684F37020000EE0002AFD3@smtp-gw.hurleymc.com>, <532021.17532.qm@web46102.mail.sp1.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D38890A1D@LRGHEXVS1.practice.lrgh.org> I went to the Navy's MLT program in 79-80 and we did drug screens manually and the chemicals that we used would give us a buzz. Thought it was ironic that I was getting high while looking for drugs in somebody else. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello [vapatpxs@yahoo.com] Sent: Thursday, July 23, 2009 1:30 PM To: Lynette Pavelich; HistoNet Subject: RE: [Histonet] Start Up Lab In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl). Several of the techs got nose bleeds every time they used the stuff. I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window. We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause "transient euphoria". No wonder we thought every thing was funny during those days. Safety people, they take all the fun out of research! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: RE: [Histonet] Start Up Lab To: cbrya@lexclin.com, Montina.VanMeter@pbrc.edu, thecitan@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 3:53 PM Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) >>> "Montina Van Meter" 07/23/09 11:31 AM >>> I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From khart <@t> gsopath.com Thu Jul 23 12:53:10 2009 From: khart <@t> gsopath.com (Kathy Hart) Date: Thu Jul 23 12:53:15 2009 Subject: [Histonet] Histotech needed Message-ID: <03B63DE44B5C014D84F9A9D8A968B51701C84211@lithium.corp.gsopath.com> Greensboro Pathology, a leading provider of pathology services for 50+ years, is currently seeking an experienced Histotechnician to work in a Chapel Hill satellite office. The Histotechnician will participate in the full cycle of dermatopathology specimen preparation from grossing through sectioning and staining. Some travel to Greensboro during initial training will be required. Requirements: * Current HT/ASCP certification preferred. * Previous sectioning & staining experience required. * Grossing experience preferred, but will train an otherwise qualified candidate. * Must have a minimum of 24 credit hours in biology, chemistry, laboratory science (or a combination thereof) to qualify for grossing tissue. Proof of credits will be required. * Effective communication skills are required. * Must be able to work independently. Please forward resume/CV to: Greensboro Pathology Attn: HR Director PO Box 13508 Greensboro, NC 27415 Fax: 336/510-0192 epancoast@gsopath.com www.greensboropathology.com Equal Opportunity Employer & Drug Free Workplace Kathy S. Hart Histology Manager Greensboro Pathology, LLC 706 Green Valley Rd. Suite 104 Greensboro, NC 27408 khart@gsopath.com phone: 336-387-2513 fax: 336-387-2563 From NSEARCY <@t> swmail.sw.org Thu Jul 23 13:01:52 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Jul 23 13:02:01 2009 Subject: [Histonet] "Like" specimens Message-ID: <4A685F40.5D38.00EF.0@swmail.sw.org> Any new and innovative ways to differentiate "like" specimens at grossing? I know that there are specialty labs (GI; prostate, etc) out thee. How do you not gross like specimens back-to-back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From CIngles <@t> uwhealth.org Thu Jul 23 13:17:07 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Jul 23 13:19:58 2009 Subject: [Histonet] "Like" specimens References: <4A685F40.5D38.00EF.0@swmail.sw.org> Message-ID: We never assession like tissues back-to-back. A different type of specimen always goes between like tissues. (except in the Dermatopathology area...) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Nita Searcy Sent: Thu 7/23/2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Like" specimens Any new and innovative ways to differentiate "like" specimens at grossing? I know that there are specialty labs (GI; prostate, etc) out thee. How do you not gross like specimens back-to-back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 From LRaff <@t> uropartners.com Thu Jul 23 13:37:02 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Jul 23 13:45:08 2009 Subject: FW: [Histonet] "Like" specimens Message-ID: We have no choice other than to put prostates back to back. To avoid mix up we use specimen inking. Our paper on the subject is in Archives of Pathology http://arpa.allenpress.com/arpaonline/?request=get-abstract&doi=10.1043% 2F1543-2165-133.2.295 Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, July 23, 2009 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Like" specimens Any new and innovative ways to differentiate "like" specimens at grossing? I know that there are specialty labs (GI; prostate, etc) out thee. How do you not gross like specimens back-to-back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 __________ Information from ESET NOD32 Antivirus, version of virus signature database 4270 (20090723) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4270 (20090723) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Thu Jul 23 14:47:09 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Jul 23 14:47:14 2009 Subject: [Histonet] Inking article Message-ID: I have received several requests for a better link to our inking article. The article title is: Lester J. Raff MD, George Engel MD, Kenneth R. Beck MD, Andrea S. O'Brien HT, ASCP and Meagan E. Bauer BA. 2009: The Effectiveness of Inking Needle Core Prostate Biopsies for Preventing Patient Specimen Identification Errors: A Technique to Address Joint Commission Patient Safety Goals in Specialty Laboratories. Archives of Pathology and Laboratory Medicine: Vol. 133, No. 2, pp. 295-297. A link that should work is: http://arpa.allenpress.com/pdfserv/10.1043%2F1543-2165-133.2.295 Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 From BBranton <@t> sarapath.com Thu Jul 23 14:50:24 2009 From: BBranton <@t> sarapath.com (Brian Branton) Date: Thu Jul 23 14:53:50 2009 Subject: [Histonet] Ventana Benchmark XT for sale Message-ID: Hello HistoNetters We are selling our Ventana Benchmark XT IHC stainer. If anyone is interested, please see the ad on our web site. http://www.sarapath.com/equipment4sale/ I would be happy to answer any questions, please feel free to email or contact me directly. Thank You Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology (941) 362-8963 (941) 362-8964 Fax From cbarone <@t> NEMOURS.ORG Thu Jul 23 16:00:44 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Jul 23 16:01:06 2009 Subject: [Histonet] RE: Histonet Digest, Vol 68, Issue 29 In-Reply-To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A748A@wlmmsx01.nemours.org> The discussion on the 70's has been very nostalgic. I also worked in a lab with an Auto-technicon, no hood, benezene and chloroform, mixed with the smell of xylene and formalin (it was the aroma therapy, of the decade for histology). We didn't use gloves to reach into the open beaker to fetch out the cassettes and plastics were done in the adjoining lab, no hood, no door. All reagents were just stored under the table...in pretty red containers and the dust from preparing fresh dyes, often dusted the open balance. My pathologist smoked in the morgue during the autopsies... and I wish I only knew then, what I know now. However, at age 61, people often compliment me on how good my skin looks. I usually reply...aroma therapy and good fixation! PS: My lab is now LEAN and Green and my tech's use all PPE. Safety in the Lab is priority one! ...and a final note, I work on an entirely smoke free campus! How the times have changed! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, July 23, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Start Up Lab (Edwards, R.E.) 2. Rat's lung (tahseen@brain.net.pk) 3. RE: Start Up Lab (Lynette Pavelich) 4. RE: Start Up Lab (Bartlett, Jeanine (CDC/CCID/NCZVED)) 5. RE: Start Up Lab (Smith, Allen) 6. RE: Start Up Lab (Bartlett, Jeanine (CDC/CCID/NCZVED)) 7. RE: Peloris tissue processor (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Jul 2009 16:51:05 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Start Up Lab To: "'Bartlett, Jeanine (CDC/CCID/NCZVED)'" , Montina Van Meter , Carol Bryant , "thecitan@yahoo.com" , Lynette Pavelich Cc: Histonet Message-ID: <7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 23 Jul 2009 21:51:16 +0600 (PKST) From: tahseen@brain.net.pk Subject: [Histonet] Rat's lung To: histonet@lists.utsouthwestern.edu Message-ID: <63947.202.125.145.178.1248364276.squirrel@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 Dear All, How the volume of Rat's lung can be meausured? Thanks advance Muhammad Tahseen Histology Supervisor SKMCH&RC lAHORE,pAKISTAN ------------------------------ Message: 3 Date: Thu, 23 Jul 2009 11:53:27 -0400 From: "Lynette Pavelich" Subject: RE: [Histonet] Start Up Lab To: ,, Cc: histonet@lists.utsouthwestern.edu Message-ID: <4A684F37020000EE0002AFD3@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) >>> "Montina Van Meter" 07/23/09 11:31 AM >>> I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 23 Jul 2009 12:13:19 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Start Up Lab To: "Edwards, R.E." , "Montina Van Meter" , "Carol Bryant" , thecitan@yahoo.com, "Lynette Pavelich" Cc: Histonet Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3400@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Hah! Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 23 Jul 2009 12:26:26 -0400 From: "Smith, Allen" Subject: RE: [Histonet] Start Up Lab To: "'Bartlett, Jeanine (CDC/CCID/NCZVED)'" Cc: "'Histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 23 Jul 2009 12:32:07 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Start Up Lab To: "Smith, Allen" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3402@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Thanks to spell-check......it self-corrected me......incorrectly! Thanks for setting it right. -----Original Message----- From: Smith, Allen [mailto:asmith@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do..... -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their "personal drawer". I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: thecitan@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thecitan@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Lynette Pavelich" Date: Wed, 22 Jul 2009 18:07:07 To: ; ; Subject: RE: [Histonet] Start Up Lab Gosh.........I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh...... >>> Merced M Leiker 07/22/09 5:00 PM >>> (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire wrote: > In the lab?!? For shame. :) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois > Sent: Wed 7/22/2009 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Start Up Lab > > > > And a coffee pot. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 23 Jul 2009 12:47:52 -0400 From: "Joyce Cline" Subject: RE: [Histonet] Peloris tissue processor To: "Histonet" Message-ID: <65281717443C4C1385063D050EB7A387@wchsys.org> Content-Type: text/plain; charset="us-ascii" We have had our Peloris since Feb. and we love it. We use the 6 hour program for our regular tissue and the 8 hour program for breast, thyroid, colon, melanoma, sentinel nodes, prostatectomy. The one hour program for prostate biopsies. We use Formula 83 instead of Xylene, or you can use Xylene free processing using Isopropyl alcohol. We like being able to run both retorts at the same time, of course they don't finish at the same time but closely. Your reagent usage drops also. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, July 22, 2009 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Anyone currently using the Peloris tissue processor please comment- love it, likes, dislikes? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 29 **************************************** From B427297 <@t> aol.com Thu Jul 23 19:02:38 2009 From: B427297 <@t> aol.com (B427297@aol.com) Date: Thu Jul 23 19:02:49 2009 Subject: [Histonet] Vet tissue processing Message-ID: I'm looking for a histology processing lab who will process NBF veterinary tissues to H+E slides. I need a short turn around time, like 7-10 days. Any suggestions will be considered. Thanks, **************Dell Deals: Treat yourself to a sweet deal on popular laptops! (http://pr.atwola.com/promoclk/100126575x1223100673x1201716527/aol?redir=http:%2F%2Faltfarm.mediaplex.com%2Fad%2Fck%2F12309%2D81939%2D1629%2D7) From conniegrubaugh <@t> hotmail.com Thu Jul 23 22:54:12 2009 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Thu Jul 23 22:54:16 2009 Subject: [Histonet] alarms on all the machines Message-ID: The other day just about every machine in the lab decided to beep and alarm all at the same time. They all sound the same!!! The stainers in histo and cyto. The processors that they were done cleaning. The alarms on the freezers ( been open too long) Both of the Venatana IHC machines and special stain timers. Plus there was a truck backing up to the door to unload something and it to was beeping. So we were wondering since cell phones have all types of different rings and tones to them couldn't someone change the beeping to something else or give us a choice. Everyone decided that I should post this on the histo net to see what everyone else thought or maybe you guys that design these machines (yes they are all wonderfull) but gives us some more sound options. Just a thought! Okay, so what does everyone think about this? Connie _________________________________________________________________ Windows Live? Hotmail?: Celebrate the moment with your favorite sports pics. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGLM_WL_QA_HM_sports_photos_072009&cat=sports From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jul 24 02:19:42 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 24 02:19:53 2009 Subject: [Histonet] Rat's lung Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0720B269@wahtntex2.waht.swest.nhs.uk> Immerse it in water fully inflated and see how much water is displaced; is that Avogadro hypothesis or some other Greek dude? Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk Sent: 23 July 2009 16:51 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat's lung Dear All, How the volume of Rat's lung can be meausured? Thanks advance Muhammad Tahseen Histology Supervisor SKMCH&RC lAHORE,pAKISTAN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abijag76 <@t> yahoo.co.in Fri Jul 24 06:43:30 2009 From: abijag76 <@t> yahoo.co.in (abi jag) Date: Fri Jul 24 06:43:38 2009 Subject: [Histonet] Oil red o_Dextrin_reg Message-ID: <413467.19317.qm@web95108.mail.in2.yahoo.com> Dear Members, I am trying to standardize the oil red o staining method in murine frozen tissues using Churukian method. I am having following queries. 1. The type and source of aqueous dextrin used in the staining procedure. Search in sigma catalogue provided different types of dextrin (dextrin from corn,maize and pottao starch etc). I want to know which should be used exactly in this procedure. Unfortunately, I am not having the article published in Journal of histotechnology to refer. Can any one please throw some light on the type of the dextrin to be used and the source to buy if any. 2. Again the procedure mentions a counter stain called acidified Lillie-Mayer hematoxylin. If there is any composition available to prepare the same or that could be purchased directly from the manufacturers (I have seen Dako is having the one). Please clarify these two things Thanks for all your help Abijag See the Web's breaking stories, chosen by people like you. Check out Yahoo! Buzz. http://in.buzz.yahoo.com/ From MAUGER <@t> email.chop.edu Fri Jul 24 07:43:52 2009 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Jul 24 07:44:18 2009 Subject: [Histonet] RE:FactorXIII In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0720B269@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0720B269@wahtntex2.waht.swest.nhs.uk> Message-ID: <4A6974480200003100009E00@email.chop.edu> Hi All, Do any of you Ventana IHC users know what pH the low pH solution actually is? Is it lower than pH6? We have a Rab Factor XIIIA from Genetex that was recommended to us. It suppossedly works on the Ventana with 'low pretreatment', but it won't work for us on the Bond with pH6 retrieval buffer. Thanks for any suggestions. Jo Mauger CHOP From leiker <@t> buffalo.edu Fri Jul 24 08:19:49 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Fri Jul 24 08:19:53 2009 Subject: [Histonet] alarms on all the machines In-Reply-To: References: Message-ID: <68DE0D495B8119A9F45CE062@CDYwxp1931.ad.med.buffalo.edu> I agree. We also have the problem with timers in our lab. Someone's goes off and we try to figure out whose it is. Couldn't they make ringtones for timers? :-) --On Thursday, July 23, 2009 8:54 PM -0700 connie grubaugh wrote: > > The other day just about every machine in the lab decided to beep and > alarm all at the same time. They all sound the same!!! The stainers in > histo and cyto. The processors that they were done cleaning. The alarms > on the freezers ( been open too long) > > Both of the Venatana IHC machines and special stain timers. Plus there > was a truck backing up to the door to unload something and it to was > beeping. So we were wondering since cell phones have all types of > different rings and tones to them couldn't someone change the beeping to > something else or give us a choice. > > Everyone decided that I should post this on the histo net to see what > everyone else thought or maybe you guys that design these machines (yes > they are all wonderfull) but gives us some more sound options. Just a > thought! Okay, so what does everyone think about this? > > > Connie > _________________________________________________________________ > Windows Live? Hotmail?: Celebrate the moment with your favorite sports > pics. Check it out. > http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGL > M_WL_QA_HM_sports_photos_072009&cat=sports_______________________________ > ________________ Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From MITCHELLJA <@t> email.chop.edu Fri Jul 24 08:28:09 2009 From: MITCHELLJA <@t> email.chop.edu (Janice Mitchell) Date: Fri Jul 24 08:28:40 2009 Subject: [Histonet] PLUS SLIDES Message-ID: <4A697EA9020000000052BF95@email.chop.edu> Good Morning Histoland, FYI. We use fisher ink-jet plus slides with clipped corners. Recently we received a case labeled correctly that were just plain slides. We did not notice it till we had used a few of the boxes of slides. It was not to big a disaster for our lab, but it might be for others. Janice From sbreeden <@t> nmda.nmsu.edu Fri Jul 24 08:35:17 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jul 24 08:35:21 2009 Subject: [Histonet] Bells & Whistles Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E469B6@nmdamailsvr.nmda.ad.nmsu.edu> I always thought it was odd that our $36000 processor (which costs more than our first HOUSE) made a grinding "blap" noise when it was done! You'd think for that amount of money you could get a pleasant (sexy) voice that says, "Good day! I have finished making your life easier. Please come and attend to me". Dontcha think?? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From jqb7 <@t> cdc.gov Fri Jul 24 08:40:30 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jul 24 08:41:15 2009 Subject: [Histonet] Bells & Whistles In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E469B6@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E469B6@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD340D@LTA3VS011.ees.hhs.gov> Absolutely! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, July 24, 2009 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bells & Whistles I always thought it was odd that our $36000 processor (which costs more than our first HOUSE) made a grinding "blap" noise when it was done! You'd think for that amount of money you could get a pleasant (sexy) voice that says, "Good day! I have finished making your life easier. Please come and attend to me". Dontcha think?? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Jul 24 08:52:07 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jul 24 08:49:51 2009 Subject: [Histonet] RE: Bells & Whistles In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E469B6@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E469B6@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088E58A216@IBMB7Exchange.digestivespecialists.com> My immuno stainer talks to me in a sexy voice! But now the issue is should you be able to request if you want a female or male voice? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, July 24, 2009 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bells & Whistles I always thought it was odd that our $36000 processor (which costs more than our first HOUSE) made a grinding "blap" noise when it was done! You'd think for that amount of money you could get a pleasant (sexy) voice that says, "Good day! I have finished making your life easier. Please come and attend to me". Dontcha think?? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Jul 24 09:11:57 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Jul 24 09:12:03 2009 Subject: [Histonet] Bells & Whistles In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E469B6@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <647198153.52461248444717070.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> For the prices we pay now they should offer to clean the lab!! Pam Marcum ----- Original Message ----- From: "Sara Breeden" To: histonet@lists.utsouthwestern.edu Sent: Friday, July 24, 2009 9:35:17 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Bells & Whistles I always thought it was odd that our $36000 processor (which costs more than our first HOUSE) made a grinding "blap" noise when it was done! You'd think for that amount of money you could get a pleasant (sexy) voice that says, "Good day! I have finished making your life easier. Please come and attend to me". ?Dontcha think?? ? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM ?87106 505-841-2576 ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Jul 24 09:23:24 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Jul 24 09:24:23 2009 Subject: [Histonet]Special Coverslips Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23B7A@PHSXMB30.partners.org> To all: I apologize for asking for this information--I deleted the email communication re: special coverslips to be used on automatic coverslippers! We have recently been approved to order a slide stainer and coverslipper and several people commented on specific types of coverglass to use. I would appreciate it if you would contact me directly (email below) re: this matter. Thank you! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From jwarren23 <@t> cinci.rr.com Fri Jul 24 09:41:26 2009 From: jwarren23 <@t> cinci.rr.com (Jean Warren) Date: Fri Jul 24 09:41:42 2009 Subject: [Histonet] Timers and bells and alarms-Oh My! Message-ID: <791D395C85C54CF9958C3C951D19C80D@ownerPC> Funny you should bring up this subject, Connie. Two of us work night shift in our Pathology department, so it should be nice and quiet! All we are supposed to do is embed and cut and stain the H and Es. Do you know how hard it is to ignore immuno and special stainers and PTS alarms and ThinPrep machines, while we are listening for our rapid tissue processor to signal that it is finished. God forbid there might be a fire alarm; we would think it was something going off in another room somewhere! Sometimes, we just have to go and silence them all. I call it putting the babies back to sleep! So yes, I wholeheartedly agree that some unique melodious sounds would be better. Jean From esther.peters <@t> verizon.net Fri Jul 24 09:42:15 2009 From: esther.peters <@t> verizon.net (Esther Peters) Date: Fri Jul 24 09:42:43 2009 Subject: [Histonet] PLUS SLIDES In-Reply-To: <4A697EA9020000000052BF95@email.chop.edu> References: <4A697EA9020000000052BF95@email.chop.edu> Message-ID: <4A69C847.9050607@verizon.net> We just received an order from Fisher in which the lab tape that was sent was 2-inches wide, but the catalog numbers on the tapes and description on the Web site was for 1-inch wide tape, so we did not get what we thought we were getting either. Esther Peters, Ph.D. George Mason University Janice Mitchell wrote: > Good Morning Histoland, FYI. We use fisher ink-jet plus slides with clipped corners. Recently we received a case labeled correctly that were just plain slides. We did not notice it till we had used a few of the boxes of slides. It was not to big a disaster for our lab, but it might be for others. Janice > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Lynn.Burton <@t> Illinois.gov Fri Jul 24 10:04:59 2009 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Jul 24 10:05:46 2009 Subject: [Histonet] Vet tissue processing References: Message-ID: We can do that but what kind of volume are you looking at? Where are you located? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 309-344-2451 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of B427297@aol.com Sent: Thu 7/23/2009 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vet tissue processing I'm looking for a histology processing lab who will process NBF veterinary tissues to H+E slides. I need a short turn around time, like 7-10 days. Any suggestions will be considered. Thanks, **************Dell Deals: Treat yourself to a sweet deal on popular laptops! (http://pr.atwola.com/promoclk/100126575x1223100673x1201716527/aol?redir=http:%2F%2Faltfarm.mediaplex.com%2Fad%2Fck%2F12309%2D81939%2D1629%2D7) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Fri Jul 24 10:30:41 2009 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Fri Jul 24 10:30:47 2009 Subject: [Histonet] GMS-Fungus on nails Message-ID: We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From jstaruk <@t> masshistology.com Fri Jul 24 10:41:05 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Fri Jul 24 10:41:03 2009 Subject: [Histonet] GMS-Fungus on nails In-Reply-To: Message-ID: Prepare a 10% solution of Titebond II premium wood glue (found in most hardware stores). Dip the slide in the solution just before mounting the section on the slide. Let the slide dry and stain away. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DStillings <@t> unipathllc.com Fri Jul 24 10:53:46 2009 From: DStillings <@t> unipathllc.com (Donella Stillings) Date: Fri Jul 24 10:53:53 2009 Subject: [Histonet] GMS-Fungus on nails In-Reply-To: References: Message-ID: <8785CEF5DCC41A4F8014179C435935D60C2EB8@exchange.unipathllc.corp> We use Albumin Solution from Sigma (A7034-10ml) on our slides. We stain on the Artisan and or the Ventana stainer as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 9:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Fri Jul 24 10:58:59 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Jul 24 10:59:04 2009 Subject: [Histonet] Peloris tissue processor In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D1557734@SJSNT-SCMAIL03.stjoe.org> Message-ID: <764853DE5F334AE9807BA4B211034778@wchsys.org> I still use my T/T mega microwave, with exchange of solutions the Peloris takes a little longer than our microwave. Yes, you can still use Xylene, Leica has protocols for Xylene usage. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Brenda <@t> nsh.org Fri Jul 24 11:11:37 2009 From: Brenda <@t> nsh.org (Brenda Royce) Date: Fri Jul 24 11:11:43 2009 Subject: [Histonet] NSH Annual Symposium Convention coming up soon! Message-ID: Happy Friday Histonet Community! The Annual NSH Symposium/Convention is fast approaching. If you haven't signed up or checked it out yet don't hesitate too long. Workshops are filling up & we don't want you to miss out on the classes you need. We know money is an issue for all labs & all families. NSH is proud of the fact that we continue to offer attendance options at all price points. You can leave the S/C earning 9 contact hours through seminars for the low price of $35 and attend the exhibit hall for free. The event only lasts 5 Days but your experiences - Networking, Learning, Discovering new products, will be Countless. This can't miss event will be held in Birmingham, AL on October 2-7, 2009. For more information visit our website www.nsh.org or call the NSH office at 443-535-4060. Spaces are limited, so register soon. We look forwarding to see you in Birmingham! Kind Regards, NSH Event Staff Looking for continuing education? Visit the NSH website . From pruegg <@t> ihctech.net Fri Jul 24 11:25:25 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 24 11:25:34 2009 Subject: [Histonet] GMS-Fungus on nails In-Reply-To: References: Message-ID: For really difficult tissues such as bone, cartilage and perhaps nails I make a 5% solution of elmers glue in dih20 and dip regular uncoated slides in the glue, let them air dry. Use the glue coated slides to pick up sections, just dipping in the water bath seems to activate the glue enough to help the sections stay on during staining. Dry the sections picked up on the glue coated slides in a 60dc oven overnight if possible or I use a slide warmer and lay them flat and dry at 55dc (that is as high as my slides warmer goes) for several hours. Good luck, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 9:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jul 24 11:27:10 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 24 11:27:21 2009 Subject: [Histonet] Vet tissue processing In-Reply-To: References: Message-ID: <71EABD1D533F45E799BC2223E367841F@ihctechq9h2qof> My company can provide that service for you. Check out my website at www.ihctech.net for a Request for Services form and list of our services and prices. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Friday, July 24, 2009 9:05 AM To: B427297@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vet tissue processing We can do that but what kind of volume are you looking at? Where are you located? Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 309-344-2451 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of B427297@aol.com Sent: Thu 7/23/2009 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vet tissue processing I'm looking for a histology processing lab who will process NBF veterinary tissues to H+E slides. I need a short turn around time, like 7-10 days. Any suggestions will be considered. Thanks, **************Dell Deals: Treat yourself to a sweet deal on popular laptops! (http://pr.atwola.com/promoclk/100126575x1223100673x1201716527/aol?redir=htt p:%2F%2Faltfarm.mediaplex.com%2Fad%2Fck%2F12309%2D81939%2D1629%2D7) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Jul 24 11:33:22 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Jul 24 11:33:29 2009 Subject: [Histonet] RELIA Histology Job Alert 2 positions open in Charlotte, NC Can you help? Message-ID: Hi Histonetters! I hope everybody is doing well and finding a way to stay cool on this hot summer day! RELIA Solutions, The nation?s only recruiting firm dedicated to the permanent placement of histology professionals is currently working with 2 premier clients in the Charlotte, NC area who are in need of ASCP certified histotechs. These are permanent full time day shift positions and my clients offer excellent salaries, benefits and relocation assistance. One of my clients is a private pathology lab that processes a variety of tissues. They are looking for a histotech with strong skills in routine histology and ASCP certification. My other client is a private derm path lab, they are looking for someone with a strong background in routine histology and derm specimens. Mohs experience is a plus and they are willing to train in Mohs technique. If you or someone you know might be interested in either of these positions please contact me-Pam Barker. I can be reached at relia1@earthlink.net or 866-607-3542. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From pruegg <@t> ihctech.net Fri Jul 24 11:33:41 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jul 24 11:33:51 2009 Subject: [Histonet] Schmorl's special stain Message-ID: <480DC73361814A6999F9195E218C38F4@ihctechq9h2qof> Does anyone have experience with this stain on ffpe rat tissue? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org From akbitting <@t> geisinger.edu Fri Jul 24 11:35:30 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Jul 24 11:35:39 2009 Subject: [Histonet] GMS-Fungus on nails In-Reply-To: References: Message-ID: <4A69AA92.2B7F.00C9.0@geisinger.edu> Just wondering why you use Titebond II? We use plain old Elmer's Glue. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "jstaruk" 7/24/2009 11:41 AM >>> Prepare a 10% solution of Titebond II premium wood glue (found in most hardware stores). Dip the slide in the solution just before mounting the section on the slide. Let the slide dry and stain away. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From rjbuesa <@t> yahoo.com Fri Jul 24 11:58:50 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 24 11:58:54 2009 Subject: [Histonet] GMS-Fungus on nails Message-ID: <651366.61406.qm@web65706.mail.ac4.yahoo.com> Under separate cover I am sinding you?my procedure. Ren? J. --- On Fri, 7/24/09, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] GMS-Fungus on nails To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, July 24, 2009, 11:30 AM We do a GMS for fungus on all nails we receive.? We often have a difficult time keeping the nail tissue on the slide.? We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck.? Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at.? We use the Artisan stainer for our specials.? We are really not interested in performing the GMS manually, due to volume and turn around time restrictions.? Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Jul 24 12:01:17 2009 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jul 24 12:01:37 2009 Subject: [Histonet] IMMUNO STAINING--DECOLORIZING Message-ID: We had a series of PIN 4 cocktail immuno stains and got a poor reaction. I am afraid to cut deeper in the block and miss the area of concern. I have determined the problem was due to the heat bar not being turned on. What can I do the slides to reverse the process and restain them. The DAB stained good, it is the vulcan fast red that was not reacting. Diana From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jul 24 12:07:26 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jul 24 12:10:59 2009 Subject: [Histonet] IMMUNO STAINING--DECOLORIZING References: Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C949D5@e2k3ms1.urmc-sh.rochester.edu> We have had similar problems with the Vulcan Fast Red not staining. It was suggested by the vendor to buy the fast red in smaller amounts (they actually repackaged the product) so that the chromogen was not being taken out of the fridge and put back in too many times. Depending on your workflow you may want to aliquot out the chromogen for each run or each couple of runs? When we see it starting to fade we use fresh fast red. That usually takes care of the problem. I have never had any luck restaining with the fast red. And I have tried many times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Diana McCaig Sent: Fri 7/24/2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMMUNO STAINING--DECOLORIZING We had a series of PIN 4 cocktail immuno stains and got a poor reaction. I am afraid to cut deeper in the block and miss the area of concern. I have determined the problem was due to the heat bar not being turned on. What can I do the slides to reverse the process and restain them. The DAB stained good, it is the vulcan fast red that was not reacting. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Jul 24 20:51:39 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jul 24 20:51:47 2009 Subject: [Histonet] GMS-Fungus on nails In-Reply-To: References: Message-ID: We soak our nails in 20% sodium hydroxide for at least an hour, rinse in running tap water for a few minutes before we place the cassettes on the tissue processor. We use positive charged slides, heat slides in 80 degree oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a lot more gentler on the tissue that the GMS. We are the toenail lab for the U.S. Air Force , Joe "The Toe" ----- Original Message ----- From: "jstaruk" To: "'Clare Thornton'" ; Sent: Friday, July 24, 2009 10:41 AM Subject: RE: [Histonet] GMS-Fungus on nails > Prepare a 10% solution of Titebond II premium wood glue (found in most > hardware stores). Dip the slide in the solution just before mounting the > section on the slide. Let the slide dry and stain away. > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare > Thornton > Sent: Friday, July 24, 2009 11:31 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] GMS-Fungus on nails > > We do a GMS for fungus on all nails we receive. We often have a difficult > time keeping the nail tissue on the slide. We've tried baking for long > periods, pre-treating in formalin, using silane slides, with no luck. > Even > when the nails cut relatively easily we still lose it, and end up running > several GMS stains before we might get a speck or two of tissue we can > look > at. We use the Artisan stainer for our specials. We are really not > interested in performing the GMS manually, due to volume and turn around > time restrictions. Any suggestions? > > Clare J. Thornton, HTL(ASCP) > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Jul 25 12:47:06 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jul 25 12:47:11 2009 Subject: [Histonet] Oil red o_Dextrin_reg In-Reply-To: <413467.19317.qm@web95108.mail.in2.yahoo.com> References: <413467.19317.qm@web95108.mail.in2.yahoo.com> Message-ID: The addition of dextrin dates from Catalano & Lillie 1975 Stain Technol. 50(5): 297-299. http://pdfserve.informaworld.com.proxy1.lib.uwo.ca:2048/882566_770885140_788730753.pdf They showed that adding dextrin more than trebled the dye content of a supersaturated solution of oil red O in 60% isopropanol, and also increased the shelf life of the solution. These authors did not say what kind of dextrin they used. Dextrin had earlier been added to Weigert-type elastin stains by French 1928 Stain Technol. 4(1): 11-12, who also did not specify a type of dextrin. Lamar Jones, in Chapter 11 of Theory and Practice of Histological Techniques (5th ed, ed. Bancroft & Gamble 2002), p.208 states: "Dextrin, bacteriological grade or Type III from Sigma, from corn starch." Does anyone know of a published comparison of different types of dextrin? John Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = ----- Original Message ----- From: abi jag Date: Friday, July 24, 2009 7:45 Subject: [Histonet] Oil red o_Dextrin_reg To: histonet@lists.utsouthwestern.edu > Dear Members, > I am trying to standardize the oil red o staining method in > murine frozen tissues using Churukian method. > > I am having following queries. > > 1. The type and source of aqueous dextrin used in the staining > procedure.Search in sigma catalogue provided different types of > dextrin (dextrin from corn,maize and pottao starch etc). I want > to know which should be used exactly in this procedure. > Unfortunately, I am not having the article published in Journal > of histotechnology to refer. Can any one please throw some light > on the type of the dextrin to be used and the source to buy if any. > > 2. Again the procedure mentions a counter stain called acidified > Lillie-Mayer hematoxylin. If there is any composition available > to prepare the same or that could be purchased directly from the > manufacturers (I have seen Dako is having the one). > > Please clarify these two things > > Thanks for all your help > > Abijag > > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jul 27 10:17:32 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jul 27 10:17:35 2009 Subject: [Histonet] RELIA Histology Careers Bulletin 7/27/09 What are you doing to stay cool during the Hot Hot Hot Dog Days of Summer? Message-ID: Hi Histonetters!! I hope you are having a great day! ! What are you doing to stay cool during these dog days of summer? I am sipping icy cold drinks and spending as much time as possible at the beach and water park!!!!!!! If you are contemplating making a job change let me help while you kick back relax and enjoy a glass of iced tea or lemonade in the shade. I can keep you posted on opportunities as they come open, assist you with your resume and coach you through the interview process. All of the positions I work with are fulltime 40 hour per week positions with top hospitals, labs and doctors offices. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance and in some cases sign on bonuses. My services are FREE of charge to you. All of my fees are paid by my clients, the facilities that I represent. I work with companies nationwide that are in need of histology supervisors, histotechnologists and histotechnicians. NEW GRADS are welcome to apply. Here is a list of my most exciting current openings: HISTOLOGY SUPERVISORS/MANAGERS Histology Supervisor ? Spokane, WA Histology Supervisor ? Los Angeles, CA Lead Histotechnologist/Shift Supervisor ? San Antonio, TX HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histotech ? Charlotte, NC Dermpath Histotech ? Charlotte, NC Histotech ? Los Angeles Histotech ? Austin, TX 2nd Shift Histotech ? North of Boston Histotech ? Upstate, NY 3rd Shift Histotech ? NYC Histotech ? Northern AL If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. Thank - Pam - 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia follow me at: www.twitter.com/pamatrelia From cbarone <@t> NEMOURS.ORG Mon Jul 27 10:45:05 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Jul 27 10:45:22 2009 Subject: [Histonet] RE: Histonet Digest, Vol 68, Issue 32 In-Reply-To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A749A@wlmmsx01.nemours.org> We have taken single IHC stains back as far as heat retrieval with excellent results. We have never done this with a cocktail...(but hey, maybe you have a technical paper in the making). The antigens most likely are still there....go back through the obvious steps, and restain using the same protocol. Don't try some other one....you will "muddle" the cells and lose crisp nuclei! Let me know how that works out with a cocktail, if you try it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, July 25, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IMMUNO STAINING--DECOLORIZING (Diana McCaig) 2. RE: IMMUNO STAINING--DECOLORIZING (McMahon, Loralee A) 3. Re: GMS-Fungus on nails (Joe Nocito) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Jul 2009 13:01:17 -0400 From: "Diana McCaig" Subject: [Histonet] IMMUNO STAINING--DECOLORIZING To: Message-ID: Content-Type: text/plain; charset="us-ascii" We had a series of PIN 4 cocktail immuno stains and got a poor reaction. I am afraid to cut deeper in the block and miss the area of concern. I have determined the problem was due to the heat bar not being turned on. What can I do the slides to reverse the process and restain them. The DAB stained good, it is the vulcan fast red that was not reacting. Diana ------------------------------ Message: 2 Date: Fri, 24 Jul 2009 13:07:26 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] IMMUNO STAINING--DECOLORIZING To: Diana McCaig , Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C949D5@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" We have had similar problems with the Vulcan Fast Red not staining. It was suggested by the vendor to buy the fast red in smaller amounts (they actually repackaged the product) so that the chromogen was not being taken out of the fridge and put back in too many times. Depending on your workflow you may want to aliquot out the chromogen for each run or each couple of runs? When we see it starting to fade we use fresh fast red. That usually takes care of the problem. I have never had any luck restaining with the fast red. And I have tried many times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Diana McCaig Sent: Fri 7/24/2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMMUNO STAINING--DECOLORIZING We had a series of PIN 4 cocktail immuno stains and got a poor reaction. I am afraid to cut deeper in the block and miss the area of concern. I have determined the problem was due to the heat bar not being turned on. What can I do the slides to reverse the process and restain them. The DAB stained good, it is the vulcan fast red that was not reacting. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 24 Jul 2009 20:51:39 -0500 From: "Joe Nocito" Subject: Re: [Histonet] GMS-Fungus on nails To: "jstaruk" , "'Clare Thornton'" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original We soak our nails in 20% sodium hydroxide for at least an hour, rinse in running tap water for a few minutes before we place the cassettes on the tissue processor. We use positive charged slides, heat slides in 80 degree oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a lot more gentler on the tissue that the GMS. We are the toenail lab for the U.S. Air Force , Joe "The Toe" ----- Original Message ----- From: "jstaruk" To: "'Clare Thornton'" ; Sent: Friday, July 24, 2009 10:41 AM Subject: RE: [Histonet] GMS-Fungus on nails > Prepare a 10% solution of Titebond II premium wood glue (found in most > hardware stores). Dip the slide in the solution just before mounting the > section on the slide. Let the slide dry and stain away. > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare > Thornton > Sent: Friday, July 24, 2009 11:31 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] GMS-Fungus on nails > > We do a GMS for fungus on all nails we receive. We often have a difficult > time keeping the nail tissue on the slide. We've tried baking for long > periods, pre-treating in formalin, using silane slides, with no luck. > Even > when the nails cut relatively easily we still lose it, and end up running > several GMS stains before we might get a speck or two of tissue we can > look > at. We use the Artisan stainer for our specials. We are really not > interested in performing the GMS manually, due to volume and turn around > time restrictions. Any suggestions? > > Clare J. Thornton, HTL(ASCP) > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 32 **************************************** From MVandeBerg <@t> chw.org Mon Jul 27 12:15:46 2009 From: MVandeBerg <@t> chw.org (Vande Berg, Mariah) Date: Mon Jul 27 12:15:50 2009 Subject: [Histonet] Razors for dissection Message-ID: Hi We are looking for razor blades to be used in specimen dissection. The tissue is frozen and will be cut by hand. So the blade is like a long straight razor with a ridge along the back that is rounded. The length I believe is 2". It will be used in a research lab. If anyone has any info or descriptions please let me know. Thanks so much! Mariah Vande Berg, HT ASCP Children's Hospital of WI From rjbuesa <@t> yahoo.com Mon Jul 27 12:22:34 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 27 12:22:38 2009 Subject: [Histonet] Razors for dissection Message-ID: <657044.39161.qm@web65713.mail.ac4.yahoo.com> Some types of very similar one-edge razor can be bought at any office supplies store, cheaper than at any medical supplier. Ren? J. --- On Mon, 7/27/09, Vande Berg, Mariah wrote: From: Vande Berg, Mariah Subject: [Histonet] Razors for dissection To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 27, 2009, 1:15 PM Hi We are looking for razor blades to be used in specimen dissection.? The tissue is frozen and will be cut by hand.? So the blade is like a long straight razor with a ridge along the back that is rounded.? The length I believe is 2".? It will be used in a research lab.? If anyone has any info or descriptions please let me know.? Thanks so much! Mariah Vande Berg, HT ASCP Children's Hospital of WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon Jul 27 12:33:56 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Jul 27 12:34:05 2009 Subject: [Histonet] Beecher tissue array maker Message-ID: Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From portera <@t> msu.edu Mon Jul 27 12:47:57 2009 From: portera <@t> msu.edu (Amy Porter) Date: Mon Jul 27 12:48:03 2009 Subject: [Histonet] Help with Connexin 43 Message-ID: Anyone out there doing FFPE IHC on rat for Connexin 43 - I am having trouble getting the titration right on this & just wondering what types of dilutions anyone is using??? I am using a standard avidin/biotin complex staining system with and endogenous AV/BI block and not getting anything of any consequence in my negative control. thanks - Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Jul 27 13:25:04 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Jul 27 13:28:14 2009 Subject: [Histonet] Beecher tissue array maker References: Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C949E2@e2k3ms1.urmc-sh.rochester.edu> I have had the incredible pleasure of trying to contact someone, anyone at beecher. It is impossible. I placed two separate orders more than 6 months ago and no one has responded to my voicemail, emails. Luckily one of the orders arrived and now we are waiting on the second order. It may be another 6 months. I have never worked with a more unresponsive company in my life. I will not purchase from them in the future. I don't recommend them to anyone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Mon 7/27/2009 1:33 PM To: histonet Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Mon Jul 27 13:35:55 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Mon Jul 27 13:36:11 2009 Subject: [Histonet] Beecher tissue array maker Message-ID: <4A6DAD3B020000DF0001A62E@gwmail.medicine.wisc.edu> I have had the same problem. It's almost as if nobody answers the phone there. They're less than 20 miles from my building, but they might as well be the Ukraine. Tom Pier >>> "McMahon, Loralee A" 07/27/09 1:31 PM >>> I have had the incredible pleasure of trying to contact someone, anyone at beecher. It is impossible. I placed two separate orders more than 6 months ago and no one has responded to my voicemail, emails. Luckily one of the orders arrived and now we are waiting on the second order. It may be another 6 months. I have never worked with a more unresponsive company in my life. I will not purchase from them in the future. I don't recommend them to anyone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Mon 7/27/2009 1:33 PM To: histonet Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Jul 27 13:43:48 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Jul 27 13:44:25 2009 Subject: [Histonet] Beecher tissue array maker References: <4A6DAD3B020000DF0001A62E@gwmail.medicine.wisc.edu> Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C949E5@e2k3ms1.urmc-sh.rochester.edu> Maybe you can drive by and knock on the door for us!!! Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: Thomas Pier [mailto:tp2@medicine.wisc.edu] Sent: Mon 7/27/2009 2:35 PM To: histonet@pathology.swmed.edu; ploykasek@phenopath.com; McMahon, Loralee A Subject: RE: [Histonet] Beecher tissue array maker I have had the same problem. It's almost as if nobody answers the phone there. They're less than 20 miles from my building, but they might as well be the Ukraine. Tom Pier >>> "McMahon, Loralee A" 07/27/09 1:31 PM >>> I have had the incredible pleasure of trying to contact someone, anyone at beecher. It is impossible. I placed two separate orders more than 6 months ago and no one has responded to my voicemail, emails. Luckily one of the orders arrived and now we are waiting on the second order. It may be another 6 months. I have never worked with a more unresponsive company in my life. I will not purchase from them in the future. I don't recommend them to anyone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Mon 7/27/2009 1:33 PM To: histonet Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Mon Jul 27 14:01:38 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Jul 27 14:01:32 2009 Subject: [Histonet] Razors for dissection In-Reply-To: Message-ID: Pathco offers a handle and 2' double-edged blade. The blades are the exact same as "carpet" blades found at Home Depot ($16/100). Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vande Berg, Mariah Sent: Monday, July 27, 2009 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Razors for dissection Hi We are looking for razor blades to be used in specimen dissection. The tissue is frozen and will be cut by hand. So the blade is like a long straight razor with a ridge along the back that is rounded. The length I believe is 2". It will be used in a research lab. If anyone has any info or descriptions please let me know. Thanks so much! Mariah Vande Berg, HT ASCP Children's Hospital of WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Mon Jul 27 14:00:09 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon Jul 27 14:02:18 2009 Subject: [Histonet] GMS-Fungus on nails References: Message-ID: We always run a PAS(manual) on our nails. Alot less harsh than GMS. We cut doubles and automatically stain them in case one falls off, and we always use plus slides. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Clare Thornton Sent: Fri 7/24/2009 10:30 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlschneider <@t> gmail.com Mon Jul 27 14:18:33 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Mon Jul 27 14:18:36 2009 Subject: [Histonet] Sakura Tissue Tek Xpress Message-ID: <1085e7000907271218j436b5db1y60a995fee852cbe2@mail.gmail.com> My group is considering the purchase of the Sakura Tissue Tek Xpress Continuous Rapid Tissue Processor. Can any Histonetters give me feedback on their experience with this instrument? I'd like to hear the good and the bad. Can you justify the cost? How did you modify your staffing schedule? Does it produce quality results on biopsies? and on surgicals? and fatty tissue? How does it effect IHC? Feel free to reply on the Histonet, or, if you'd prefer, you may email me privately. Thank you! Daniel Schneider, MD From lpwenk <@t> sbcglobal.net Mon Jul 27 20:35:10 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jul 27 20:35:23 2009 Subject: [Histonet] Workshop Message-ID: Would like some input from Histonetters, sent directly to my home email, not through Histonet. Lpwenk@sbcglobal.net I'm giving a workshop at NSH this year on cultural and religious considerations in the laboratory, along with a minister who is in charge of our hospital's pastoral care education. He gave a 30 minute talk at our state meeting a few years ago, and everyone thought he and the information was great. Once or twice, he's talked to our MT and HT/HTL students about this topic, and we're including him again this year in our student's management talks. NSH has accepted this as a 3 hour workshop. We've been putting together information about different religons and cultures, in relation to laboratories (blood transfusions, transplants, autopsies, genetics, fetuses, placentas, burial, etc.). We're going to concentrate on what we as laboratorians can do to respect others' beliefs and traditions. If you have an example of some religious or cultural consideration that you or your lab have done in the past, and how you handled it, would you mind passing it along to my home email? Michigan has a lot of people from all over the world, and our hospital and lab have tried to become aware of the needs of our patients and their families in terms of religious and cultural wishes. But Michigan may not have a high enough population for our hospital/lab to have experience with all cultures, nationalities, religions, etc. For example, I should imagine the US states on the west coast have more experience with people from the Polynesian Islands than we do. ANative American traditions differ by location. And I'd love histonetters from other countries to chime in also. We know we can't cover every single religion or nationality, and we can't cover the spectrum of differences within each religion and culture. But we're going to cover the main considerations, and how the laboratories can accommodate our patients. We're going to cover the typical Anatomic Pathology labs (histology, autopsy) but will also cover some Clinical Pathology labs (phlebotomy, blood transfusions, genetics, etc.), so that participants can take the document back to their place of employment, and start a dialog with all the labs. Thanks in advance for your help. Peggy Wenk Lpwenk@sbcglobal.net From p_bourne_14526 <@t> yahoo.com Mon Jul 27 23:06:34 2009 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Mon Jul 27 23:06:41 2009 Subject: [Histonet] Beecher tissue array maker In-Reply-To: References: Message-ID: <67731.79146.qm@web51703.mail.re2.yahoo.com> This company is a very small group who do not have the support staff to answer the phones, emails etc.? However, they are very much in demand.? I was always successful dealing with them by leaving a voice mail and letting them know that my order was urgent.? I would fax my orders directly and the product usually came once I started this way of ordering.? They are most likely over-whelmed with the success of their idea.? I also double ordered to avoid running out of TMA needles.? ________________________________ From: Patti Loykasek To: histonet Sent: Monday, July 27, 2009 10:33:56 AM Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.PALMER <@t> svhm.org.au Tue Jul 28 00:25:50 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Tue Jul 28 00:25:57 2009 Subject: [Histonet] resins for embedding of PEG scaffolds Message-ID: Hello all. I am looking for a non-paraffin embedding medium for some rat tissues which have grown into a polyethylene glycol-based porous tissue engineering scaffold (a second component of the scaffold is PCL, polycaprolactone). Being PEG-based, the scaffolds are essentially hydrophilic in nature. Standard paraffin embedding gives sections which are somewhat crumbly and which adhere very poorly to slides for both routine and immunostaining, whether they are coated with APES, poly-L-lysine or chrome gelatin. Hence I wish to try something else. Have tried Technovit 8100, ie a glycol methacrylate, but this led to unacceptable distortion / diffusion of the scaffold material, something which we do not see with paraffin. I don't have much experience with the different resins, but was wondering if one of the epoxy media - hydrophobic rather than the hydrophilic methacrylates - might be worth a go? We want to do light microscopy, not EM, on the samples - at least H&E's or toluidine blue, but immunostaining would be great too. Any thoughts appreciated. Thanks a bunch! Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jwarren23 <@t> cinci.rr.com Tue Jul 28 07:20:54 2009 From: jwarren23 <@t> cinci.rr.com (Jean Warren) Date: Tue Jul 28 07:21:22 2009 Subject: [Histonet] Sakura Rapid Tissue Processor Message-ID: We have the Sakura rapid tissue processor at my hospital lab, which is a large private hospital. We have had it three years and it has been somewhat of a disappointment:: We have been told that you should not process breasts in it, because you will not get reliable results for FISH. Its implementation has created schedule changes that have caused some good techs to leave. The scenario to get a case out the same day is rare. If a patient has surgery at 7 am and we receive it by 8 am, it is accessioned and grossed in. Except for biopsies, the specimen still will need 2 hours fixation in formalin. When we receive it in Histology at 1030 am, it must go in pre-processing solution for 30 minutes. At 1100, we process it for approx 1 hour. Then embed, cut and stain. Our docs would get it well after lunch and, if all is OK, they can get the report out. And, there are not many cases that meet that time criterion. One other drawback is that it is more labor intensive to handle 10 blocks ten times a day than to handle 100 at one time. Our lab processes from 400-750 blocks a day and less than 100 a day are processed on that processor. All we are handling on the instrument are bxs, bone marrows and cytology blocks. If too large a specimen is placed on it, we usually have a problem. Endometrial bxs, skins and cones have not had favorable results. On the other hand, biopsies, especially livers, look and cut better. Our hematology expert wants all bone marrows done that way. We also rapid process most cytology that way, but bloody cases still need formalin fixation. It is much simpler to change the processor ( and more expensive ). We tried to do most specimens on it with terrible failure. If anyone gets it, I would recommend starting slowly with a few specimen types and gradually adding. Our hope is that it will be more useful in the future. I know, rather wordy answer! From sbreeden <@t> nmda.nmsu.edu Tue Jul 28 07:36:03 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jul 28 07:36:09 2009 Subject: [Histonet] Minnows? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E469C2@nmdamailsvr.nmda.ad.nmsu.edu> I've got two silvery minnows to deal with. One is about ?" and one 1". I've not done fish and am not sure if I should decal them first and try to process them whole or what! A whole mount would be interesting to look at but maybe that's not the best way. I can't imagine dissecting out tiny little organs... Anyone have any experience with tiny fish? I'd appreciate any clues! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From gibiopsypath <@t> aol.com Tue Jul 28 08:09:35 2009 From: gibiopsypath <@t> aol.com (gibiopsypath@aol.com) Date: Tue Jul 28 08:09:54 2009 Subject: [Histonet] Pathologist needed Message-ID: <8CBDD9BCA3BBDF6-D1C-487@webmail-dh36.sysops.aol.com> A private, well established, Ohio private pathology lab is looking for a pathologist for a busy GI practice.? The salary is excellent and comes with a highly efficient lab team.? For more information send a CV to this email address.? All inquiries will be kept confidential.? From kgrobert <@t> rci.rutgers.edu Tue Jul 28 09:09:50 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Tue Jul 28 08:55:39 2009 Subject: [Histonet] Minnows? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E469C2@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E469C2@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4A6F06AE.60300@rci.rutgers.edu> I get everything from mice to birds to fish (zebrafish, but close enough for our purposes), with the occasional larger animal thrown in (deer, horse). Since I'm in academic research, things are different for me-I receive instructions on what to do with every tissue sample submitted, so if I were you, I would first check with the person/organization that sent them and ask them what they want. Likely you will have to decal them, process them whole, and cut them to the level that they want. Good luck! Kathleen Roberts Principal Lab Technician Neurotoxicology Laboratories Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 (732) 445-6914 FAX (732) 445-6905 Breeden, Sara wrote: >I've got two silvery minnows to deal with. One is about ?" and one 1". I've not done fish and am not sure if I should decal them first and try to process them whole or what! A whole mount would be interesting to look at but maybe that's not the best way. I can't imagine dissecting out tiny little organs... Anyone have any experience with tiny fish? I'd appreciate any clues! > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From dlschneider <@t> gmail.com Tue Jul 28 09:01:36 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Jul 28 09:01:42 2009 Subject: [Histonet] Sakura Rapid Tissue Processor In-Reply-To: References: Message-ID: <1085e7000907280701p5da3924bg7be9c39ded659bb@mail.gmail.com> Actually, that was perfect, wordy replies are what I had hoped for. How is the automated embedding working out for you? The way I see things, automated embedding is the true killer feature of the Xpress -- that is, if it works. Why don't skins process well on the Xpress? (When I first appreciated the automated embedding feature, my first thought was "Great, no more misembedded pigmented skin lesions!" But if we can't do skins, well...) Breasts cancers require at least 8 hours formalin fixation for reliable Her2Neu FISH; presumably that's why you've been told not to do breasts on the Xpress. That said, is there a reason why we couldn't or shouldn't process breast core Bx's on the Xpress provided they've been sitting overnight in formalin? Thanks!!! Daniel Schneider On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren wrote: > We have the Sakura rapid tissue processor at my hospital lab, which is a > large private hospital. We have had it three years and it has been somewhat > of a disappointment:: > > We have been told that you should not process breasts in it, because you > will not get reliable > results for FISH. > > Its implementation has created schedule changes that have caused some good > techs to leave. > > The scenario to get a case out the same day is rare. > If a patient has surgery at 7 am and we receive it by 8 am, it is > accessioned > and grossed in. > Except for biopsies, the specimen still will need 2 hours fixation in > formalin. When > we receive it in Histology at 1030 am, it must go in pre-processing > solution > for 30 minutes. At 1100, we process it for approx 1 hour. > Then embed, cut and stain. Our docs would get it well after lunch and, if > all is > OK, they can get the report out. > And, there are not many cases that meet that time criterion. > > One other drawback is that it is more labor intensive to handle 10 blocks > ten times a day than to handle 100 at one time. Our lab processes from > 400-750 blocks a day and less than 100 a day are processed on that > processor. All we are handling on the instrument are bxs, bone marrows and > cytology blocks. If too large a specimen is placed on it, we usually have a > problem. Endometrial bxs, skins and cones have not had favorable results. > > On the other hand, biopsies, especially livers, look and cut better. Our > hematology expert wants all bone marrows done that way. We also rapid > process most cytology that way, but bloody cases still need formalin > fixation. > > It is much simpler to change the processor ( and more expensive ). > > We tried to do most specimens on it with terrible failure. If anyone gets > it, I > would recommend starting slowly with a few specimen types and gradually > adding. > > Our hope is that it will be more useful in the future. > > I know, rather wordy answer! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbreeden <@t> nmda.nmsu.edu Tue Jul 28 09:17:33 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jul 28 09:17:37 2009 Subject: [Histonet] Fishy Notes Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E469CF@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to everyone that responded to my Fishy Predicament! I have lots of hints, clues, procedures and background on how to cut these little silvery minnows and I appreciate every single one of you for responding. In cases like this, I choose to go directly to the Histo Experts and I'm always steered in the right direction. So - thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From bhewlett <@t> cogeco.ca Tue Jul 28 09:36:19 2009 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Jul 28 09:36:22 2009 Subject: [Histonet] Sakura Rapid Tissue Processor References: <1085e7000907280701p5da3924bg7be9c39ded659bb@mail.gmail.com> Message-ID: Daniel, You wrote; >>That said, is there a reason why we couldn't or shouldn't > process breast core Bx's on the Xpress provided they've been sitting > overnight in formalin? See following article. Concensus Recomendations on ER testing in breast cancer by IHC(Appl Immunohistochem Mol Morphol 2008;16:513-520) Bryan ---- Original Message ----- From: "Daniel Schneider" To: "Jean Warren" Cc: "histonet" Sent: Tuesday, July 28, 2009 10:01 AM Subject: Re: [Histonet] Sakura Rapid Tissue Processor > Actually, that was perfect, wordy replies are what I had hoped for. > > How is the automated embedding working out for you? The way I see things, > automated embedding is the true killer feature of the Xpress -- that is, > if > it works. > > Why don't skins process well on the Xpress? (When I first appreciated the > automated embedding feature, my first thought was "Great, no more > misembedded pigmented skin lesions!" But if we can't do skins, well...) > > Breasts cancers require at least 8 hours formalin fixation for reliable > Her2Neu FISH; presumably that's why you've been told not to do breasts on > the Xpress. That said, is there a reason why we couldn't or shouldn't > process breast core Bx's on the Xpress provided they've been sitting > overnight in formalin? > Thanks!!! > Daniel Schneider > On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren > wrote: > >> We have the Sakura rapid tissue processor at my hospital lab, which is a >> large private hospital. We have had it three years and it has been >> somewhat >> of a disappointment:: >> >> We have been told that you should not process breasts in it, because you >> will not get reliable >> results for FISH. >> >> Its implementation has created schedule changes that have caused some >> good >> techs to leave. >> >> The scenario to get a case out the same day is rare. >> If a patient has surgery at 7 am and we receive it by 8 am, it is >> accessioned >> and grossed in. >> Except for biopsies, the specimen still will need 2 hours fixation in >> formalin. When >> we receive it in Histology at 1030 am, it must go in pre-processing >> solution >> for 30 minutes. At 1100, we process it for approx 1 hour. >> Then embed, cut and stain. Our docs would get it well after lunch and, if >> all is >> OK, they can get the report out. >> And, there are not many cases that meet that time criterion. >> >> One other drawback is that it is more labor intensive to handle 10 blocks >> ten times a day than to handle 100 at one time. Our lab processes from >> 400-750 blocks a day and less than 100 a day are processed on that >> processor. All we are handling on the instrument are bxs, bone marrows >> and >> cytology blocks. If too large a specimen is placed on it, we usually >> have a >> problem. Endometrial bxs, skins and cones have not had favorable results. >> >> On the other hand, biopsies, especially livers, look and cut better. Our >> hematology expert wants all bone marrows done that way. We also rapid >> process most cytology that way, but bloody cases still need formalin >> fixation. >> >> It is much simpler to change the processor ( and more expensive ). >> >> We tried to do most specimens on it with terrible failure. If anyone gets >> it, I >> would recommend starting slowly with a few specimen types and gradually >> adding. >> >> Our hope is that it will be more useful in the future. >> >> I know, rather wordy answer! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AHutton <@t> dh.org Tue Jul 28 09:41:04 2009 From: AHutton <@t> dh.org (Hutton, Allison) Date: Tue Jul 28 09:42:39 2009 Subject: [Histonet] computer order enrty Message-ID: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> We are currently looking into computer order entry for our pathology specimens (using meditech). However, our major concern is that the specimens will be assigned a surgical number when the specimen is ordered in the OR, GI, radiology, etc and like specimens may not be separated. Does anyone utilize the order entry module, and if so, how do you prevent like specimens for getting back to back numbers? Thanks in advance, Allison From tahseen <@t> brain.net.pk Tue Jul 28 09:57:49 2009 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Tue Jul 28 09:57:58 2009 Subject: [Histonet] Temperature of the dead body storage refrigerator Message-ID: <22066.202.125.145.178.1248793069.squirrel@brain.net.pk> Dear all, The temperature of the dead body storage refrigerator was fluctuating up to 6 and 7 centigrade. I am looking referances if any ? Thanks advance Muhammad Tahseen Histology Supervisor SKMCH&RC LAHORE,PAKISTAN From rjbuesa <@t> yahoo.com Tue Jul 28 10:03:20 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 28 10:03:26 2009 Subject: [Histonet] Sakura Rapid Tissue Processor Message-ID: <930240.62689.qm@web65707.mail.ac4.yahoo.com> Daniel: The other part of your question refers to costs. Under separate cover I am sending you an article I wrote on the cost effectiveness of the Xpress and other MW technology tissue processor (although the Xpress is an hybrid). Please also pay attention to the previous answer where it is stated that the schedules changes (which can be "drastic") caused the lost of some good technicians. Many things have to be considered when such a change is planned. Ren? J. --- On Tue, 7/28/09, Daniel Schneider wrote: From: Daniel Schneider Subject: Re: [Histonet] Sakura Rapid Tissue Processor To: "Jean Warren" Cc: "histonet" Date: Tuesday, July 28, 2009, 10:01 AM Actually, that was perfect, wordy replies are what I had hoped for. How is the automated embedding working out for you?? The way I see things, automated embedding is the true killer feature of the Xpress -- that is, if it works. Why don't skins process well on the Xpress?? (When I first appreciated the automated embedding feature, my first thought was "Great, no more misembedded pigmented skin lesions!"? But if we can't do skins, well...) Breasts cancers require at least 8 hours formalin fixation for reliable Her2Neu FISH; presumably that's why you've been told not to do breasts on the Xpress.? That said, is there a reason why we couldn't or shouldn't process breast core Bx's on the Xpress provided they've been sitting overnight in formalin? Thanks!!! Daniel Schneider On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren wrote: > We have the Sakura rapid tissue processor at my hospital lab, which is a > large private hospital. We have had it three years and it has been somewhat > of a disappointment:: > > We have been told that you should not process breasts in it, because you > will not get reliable > results for FISH. > > Its implementation has created schedule changes that have caused some good > techs to leave. > > The scenario to get a case out the same day is rare. > If a patient has surgery at 7 am and we receive it by 8 am, it is > accessioned > and grossed in. > Except for biopsies, the specimen still will need 2 hours fixation in > formalin. When > we receive it in Histology at 1030 am, it must go in pre-processing > solution > for 30 minutes. At 1100, we process it for approx 1 hour. > Then embed, cut and stain. Our docs would get it well after lunch and, if > all is > OK, they can get the report out. > And, there are not many cases that meet that time criterion. > > One other drawback is that it is more labor intensive to handle 10 blocks > ten times a day than to handle 100 at one time. Our lab processes from > 400-750 blocks a day and less than 100 a day are processed on that > processor. All we are handling on the instrument are bxs, bone marrows and > cytology blocks. If too large a specimen is placed on it,? we usually have a > problem. Endometrial bxs, skins and cones have not had favorable results. > > On the other hand, biopsies, especially livers, look and cut better. Our > hematology expert wants all bone marrows done that way. We also rapid > process most cytology that way, but bloody cases still need formalin > fixation. > > It is much simpler to change the processor ( and more expensive ). > > We tried to do most specimens on it with terrible failure. If anyone gets > it, I > would recommend starting slowly with a few specimen types and gradually > adding. > > Our hope is that it will be more useful in the future. > > I know, rather wordy answer! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beth.Fye <@t> HCAhealthcare.com Tue Jul 28 10:51:46 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Tue Jul 28 10:51:55 2009 Subject: [Histonet] FW: Order Entry - Meditech Message-ID: <938F8EC5A524D34EB5796E23E52781D32925DFA4B8@NADCWPMSGCMS05.hca.corpad.net> Allison, We have not implemented Order Entry for Surgical Pathology as of yet, but it is on the wish list for later this year. We do however, currently do our Cytology Specimens this way. The case is ordered under OE but it is still accessioned once received in the laboratory, so the accession number is assigned there. That would allow you to keep like cases separated. Please keep me informed of your progress, I would love to learn from your experience. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From mike <@t> pathview.com Tue Jul 28 08:10:34 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Jul 28 11:16:46 2009 Subject: [Histonet] FW: Order Entry - Meditech In-Reply-To: <938F8EC5A524D34EB5796E23E52781D32925DFA4B8@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D32925DFA4B8@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <003c01ca0f84$d1811d80$74835880$@com> I think I know the answer, but at the risk of sounding quite ignorant, can you guys tell me why you'd want to separate 'like' specimen? To me, at least, manually accessioning a case is an awful steep price to pay for this desire. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Tuesday, July 28, 2009 10:52 AM To: histonet@lists.utsouthwestern.edu Cc: AHutton@dh.org Subject: [Histonet] FW: Order Entry - Meditech Allison, We have not implemented Order Entry for Surgical Pathology as of yet, but it is on the wish list for later this year. We do however, currently do our Cytology Specimens this way. The case is ordered under OE but it is still accessioned once received in the laboratory, so the accession number is assigned there. That would allow you to keep like cases separated. Please keep me informed of your progress, I would love to learn from your experience. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Tue Jul 28 11:28:45 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Jul 28 11:28:50 2009 Subject: [Histonet] RE: computer order entry - Meditech In-Reply-To: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> Message-ID: We have Meditech here. Our lab secretaries accession all cases - not the individual departments. We do not use OE for this. After the secretaries have ordered the specimens, I do go back into each case to be sure that the correct tissue description and CPT code have been entered. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center P. O. Box 547 Barre, VT 05641 802-371-4923 From Joseph.Jurcisek <@t> nationwidechildrens.org Tue Jul 28 12:11:52 2009 From: Joseph.Jurcisek <@t> nationwidechildrens.org (Jurcisek, Joseph) Date: Tue Jul 28 12:12:08 2009 Subject: [Histonet] immunofluorescence labeling on JB-4 embedded tissues Message-ID: I need to run some immunofluorescent labeling on some tissue which was embedded in JB-4. Does anyone have a protocol? My boss thinks I need to etch the plastic beforehand. Thanks. Joe Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis The Research Institute at Nationwide Children's Hospital 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 Joseph.Jurcisek@nationwidechildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Popp.Laurie <@t> mayo.edu Tue Jul 28 12:48:19 2009 From: Popp.Laurie <@t> mayo.edu (Popp, Laurie A.) Date: Tue Jul 28 12:48:24 2009 Subject: [Histonet] RE: Beecher Instruments In-Reply-To: References: Message-ID: <1488342ADB74EC4E969C48BDCDE0BD9801DBF25A@msgebe23.mfad.mfroot.org> Good luck. Rep was supposed to show up here yesterday and has yet to arrive or call. We're looking for a different company to work with. Anyone with suggestions other than a manual arrayer... Right now we're looking at going semi-automatic to get away from working with them. Laurie Laurie Popp, BA HT (ASCP) TACMA Shared Resources Mayo Clinic, Rochester, MN From TMcNemar <@t> lmhealth.org Tue Jul 28 13:06:05 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Jul 28 13:06:15 2009 Subject: [Histonet] FW: Order Entry - Meditech In-Reply-To: <003c01ca0f84$d1811d80$74835880$@com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E114@lmhsmail.lmhealth.org> We do not use OE. Histo accessions all cytology and surgical specimens. We separate 'like' specimens in case there is a number mix up. We get a lot of GI's and skins and just consider it good practice to accession a different specimen type in between. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michael Mihalik Sent: Tuesday, July 28, 2009 9:11 AM To: 'Fye Beth'; histonet@lists.utsouthwestern.edu Cc: AHutton@dh.org Subject: RE: [Histonet] FW: Order Entry - Meditech I think I know the answer, but at the risk of sounding quite ignorant, can you guys tell me why you'd want to separate 'like' specimen? To me, at least, manually accessioning a case is an awful steep price to pay for this desire. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Tuesday, July 28, 2009 10:52 AM To: histonet@lists.utsouthwestern.edu Cc: AHutton@dh.org Subject: [Histonet] FW: Order Entry - Meditech Allison, We have not implemented Order Entry for Surgical Pathology as of yet, but it is on the wish list for later this year. We do however, currently do our Cytology Specimens this way. The case is ordered under OE but it is still accessioned once received in the laboratory, so the accession number is assigned there. That would allow you to keep like cases separated. Please keep me informed of your progress, I would love to learn from your experience. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Jul 28 13:16:23 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jul 28 13:16:30 2009 Subject: [Histonet] computer order enrty In-Reply-To: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83374@EMAIL.archildrens.org> We are hoping to do the same here. But OE does not assign a specimen number. The specimen number would be assigned when you receive the specimen. As for like specimens, we have to accession them together as we get so many GI bx's that's it's impossible to separate them. We do use a color code system to separate the biopsy cases. We use 6 different alternating colors and the color is dictated in the gross description. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, July 28, 2009 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] computer order enrty We are currently looking into computer order entry for our pathology specimens (using meditech). However, our major concern is that the specimens will be assigned a surgical number when the specimen is ordered in the OR, GI, radiology, etc and like specimens may not be separated. Does anyone utilize the order entry module, and if so, how do you prevent like specimens for getting back to back numbers? Thanks in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From alyssa <@t> alliedsearchpartners.com Tue Jul 28 13:29:09 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Jul 28 13:29:14 2009 Subject: [Histonet] Dermatopathologist Needed-Permanent Message-ID: Hello All, I have a position for a Dermatopathologist in Phoenix, AZ with a well established client of mine. This position is Permanent/Direct Hire, fully benefited, and full time. The details are below. *We do offer a cash bonus for referrals if we place one of your referrals in a position, so please forward this to anyone who may be fit for a position like this and have them send me their updated resume. * *Position:* Dermatopathologist Full Time/Permanent Direct Hire *Looking for Local Candidates or Candidate Who Does Not Need Relocation Assistance As Relocation Expenses Not Offered* *Benefits:* Highly competitive compensation package, compensation continuously reviewed and updated. Medical Benefits, and much more! *Facility:* Private clinical laboratory *How to apply:* Please respond with updated resume for prescreening purposes *All inquiries kept confidential* Have a great day everyone! Respectfully, -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From ASelf <@t> georgetownhospitalsystem.org Tue Jul 28 13:38:18 2009 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Jul 28 13:38:23 2009 Subject: [Histonet] General Orientation Checklist Message-ID: Good Day Histonetters, Does anyone have a general orientation checklist for histology for new hires that they would be willing to share with me? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From jessgrocki <@t> yahoo.com Tue Jul 28 13:44:43 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Jul 28 13:44:46 2009 Subject: [Histonet] Biocare IntelliPATH Flex Immunostainer Message-ID: <70079.51422.qm@web82008.mail.mud.yahoo.com> ? Hi All, We are just wondering if anyone out there is using the Biocare IntelliPATH Flex Immunostainer? If so how do you like it? Any problems? How long have you had it? And any other information you could provide about it would be great. Thanks, Jessica Piche-Grocki, HT(ASCP) From victor <@t> pathology.washington.edu Tue Jul 28 13:50:00 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jul 28 13:50:06 2009 Subject: [Histonet] computer order enrty In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83374@EMAIL.archildrens.org> References: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83374@EMAIL.archildrens.org> Message-ID: <4A6F4858.7070605@pathology.washington.edu> I thought I'd give you my observations on OE. I don't know anything about Meditech, but with PowerPath it can be very nice. We have established OE with EPIC. When we receive the specimen, we enter the order number and virtually every field you need is filled in, patient demographics, referring physician, specimen and clinical information. Our accessioner just verifies the information, sometimes the specimen needs to be changed to pull in the correct lab orders. It is a great time saver. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Horn, Hazel V wrote: > We are hoping to do the same here. But OE does not assign a specimen > number. The specimen number would be assigned when you receive the > specimen. > > As for like specimens, we have to accession them together as we get so > many GI bx's that's it's impossible to separate them. We do use a > color code system to separate the biopsy cases. We use 6 different > alternating colors and the color is dictated in the gross description. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, > Allison > Sent: Tuesday, July 28, 2009 9:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] computer order enrty > > We are currently looking into computer order entry for our pathology > specimens (using meditech). However, our major concern is that the > specimens will be assigned a surgical number when the specimen is > ordered in the OR, GI, radiology, etc and like specimens may not be > separated. Does anyone utilize the order entry module, and if so, how > do you prevent like specimens for getting back to back numbers? > Thanks in advance, > Allison > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Tue Jul 28 13:55:06 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jul 28 13:52:35 2009 Subject: [Histonet] Biocare IntelliPATH Flex Immunostainer In-Reply-To: <70079.51422.qm@web82008.mail.mud.yahoo.com> References: <70079.51422.qm@web82008.mail.mud.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39088E58A252@IBMB7Exchange.digestivespecialists.com> Jessica, I have had the Bio-Care Intellipath stainer since the middle of December and love it. If you would like to contact me off line I would be more than happy to answer any questions you have. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Tuesday, July 28, 2009 2:45 PM To: histonet Subject: [Histonet] Biocare IntelliPATH Flex Immunostainer ? Hi All, We are just wondering if anyone out there is using the Biocare IntelliPATH Flex Immunostainer? If so how do you like it? Any problems? How long have you had it? And any other information you could provide about it would be great. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MVandeBerg <@t> chw.org Tue Jul 28 14:14:30 2009 From: MVandeBerg <@t> chw.org (Vande Berg, Mariah) Date: Tue Jul 28 14:14:34 2009 Subject: [Histonet] (no subject) Message-ID: Hi; Is there any lab out there in need of a Path. Assistant? We have an interested P.A. Let me know. mariah Children's Hospital of WI From Popp.Laurie <@t> mayo.edu Tue Jul 28 14:39:22 2009 From: Popp.Laurie <@t> mayo.edu (Popp, Laurie A.) Date: Tue Jul 28 14:39:26 2009 Subject: [Histonet] Recall: Beecher Instruments Message-ID: <1488342ADB74EC4E969C48BDCDE0BD9801DBF25C@msgebe23.mfad.mfroot.org> Popp, Laurie A. would like to recall the message, " Beecher Instruments". From mike <@t> pathview.com Tue Jul 28 14:56:27 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Jul 28 14:54:40 2009 Subject: [Histonet] computer order enrty In-Reply-To: <4A6F4858.7070605@pathology.washington.edu> References: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83374@EMAIL.archildrens.org> <4A6F4858.7070605@pathology.washington.edu> Message-ID: <001e01ca0fbd$8bb6f7a0$a324e6e0$@com> Victor, just as an fyi, in our system, THAT's when we create an accession number. Prior to this point, it's only an ORDER #. This gives the lab the flexibility to separate 'like' accn #'s, plus some other things. I concur that the benefits of an order entry interface are HUGE. The only caveat is that you usually have to 'massage' the surgical case data. For pap smears, order entry data is often times 100% accurate. But I'd still like to hear the 'details' about why it's commonplace to separate like specimen. I have a suspicion that this would no longer be needed if all materials are bar coded, but I need to hear the details behind this policy in order to make up my mind. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, July 28, 2009 1:50 PM To: Horn, Hazel V Cc: histonet@lists.utsouthwestern.edu; Hutton, Allison Subject: Re: [Histonet] computer order enrty I thought I'd give you my observations on OE. I don't know anything about Meditech, but with PowerPath it can be very nice. We have established OE with EPIC. When we receive the specimen, we enter the order number and virtually every field you need is filled in, patient demographics, referring physician, specimen and clinical information. Our accessioner just verifies the information, sometimes the specimen needs to be changed to pull in the correct lab orders. It is a great time saver. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Horn, Hazel V wrote: > We are hoping to do the same here. But OE does not assign a specimen > number. The specimen number would be assigned when you receive the > specimen. > > As for like specimens, we have to accession them together as we get so > many GI bx's that's it's impossible to separate them. We do use a > color code system to separate the biopsy cases. We use 6 different > alternating colors and the color is dictated in the gross description. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, > Allison > Sent: Tuesday, July 28, 2009 9:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] computer order enrty > > We are currently looking into computer order entry for our pathology > specimens (using meditech). However, our major concern is that the > specimens will be assigned a surgical number when the specimen is > ordered in the OR, GI, radiology, etc and like specimens may not be > separated. Does anyone utilize the order entry module, and if so, how > do you prevent like specimens for getting back to back numbers? > Thanks in advance, > Allison > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Jul 28 14:59:40 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jul 28 14:59:30 2009 Subject: [Histonet] computer order enrty In-Reply-To: <001e01ca0fbd$8bb6f7a0$a324e6e0$@com> References: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83374@EMAIL.archildrens.org><4A6F4858.7070605@pathology.washington.edu> <001e01ca0fbd$8bb6f7a0$a324e6e0$@com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA56AB80C@ITSSSXM01V6.one.ads.che.org> Yes, Michael. Barcoding would eliminate the necessity of separating. That's an old rule/habit that many of us have because it is easier to sort out the errors if numbering gets messed up. When you are in a lab of all like specimens there is not that option, of course. So you stay up all night worrying about mixing up specimens. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Tuesday, July 28, 2009 15:56 To: 'Victor Tobias'; 'Horn, Hazel V' Cc: histonet@lists.utsouthwestern.edu; 'Hutton, Allison' Subject: RE: [Histonet] computer order enrty Victor, just as an fyi, in our system, THAT's when we create an accession number. Prior to this point, it's only an ORDER #. This gives the lab the flexibility to separate 'like' accn #'s, plus some other things. I concur that the benefits of an order entry interface are HUGE. The only caveat is that you usually have to 'massage' the surgical case data. For pap smears, order entry data is often times 100% accurate. But I'd still like to hear the 'details' about why it's commonplace to separate like specimen. I have a suspicion that this would no longer be needed if all materials are bar coded, but I need to hear the details behind this policy in order to make up my mind. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, July 28, 2009 1:50 PM To: Horn, Hazel V Cc: histonet@lists.utsouthwestern.edu; Hutton, Allison Subject: Re: [Histonet] computer order enrty I thought I'd give you my observations on OE. I don't know anything about Meditech, but with PowerPath it can be very nice. We have established OE with EPIC. When we receive the specimen, we enter the order number and virtually every field you need is filled in, patient demographics, referring physician, specimen and clinical information. Our accessioner just verifies the information, sometimes the specimen needs to be changed to pull in the correct lab orders. It is a great time saver. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Horn, Hazel V wrote: > We are hoping to do the same here. But OE does not assign a specimen > number. The specimen number would be assigned when you receive the > specimen. > > As for like specimens, we have to accession them together as we get so > many GI bx's that's it's impossible to separate them. We do use a > color code system to separate the biopsy cases. We use 6 different > alternating colors and the color is dictated in the gross description. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Hutton, Allison > Sent: Tuesday, July 28, 2009 9:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] computer order enrty > > We are currently looking into computer order entry for our pathology > specimens (using meditech). However, our major concern is that the > specimens will be assigned a surgical number when the specimen is > ordered in the OR, GI, radiology, etc and like specimens may not be > separated. Does anyone utilize the order entry module, and if so, how > do you prevent like specimens for getting back to back numbers? > Thanks in advance, > Allison > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ********************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not > the intended recipient, or an employee or agent responsible for > delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Jackie.O'Connor <@t> abbott.com Tue Jul 28 15:29:37 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jul 28 15:29:54 2009 Subject: [Histonet] Biocare IntelliPATH Flex Immunostainer In-Reply-To: <70079.51422.qm@web82008.mail.mud.yahoo.com> Message-ID: We have had it since February-ish. Love it. No problems. Great customer service. It talks to you, too - like my GPS. Jackie O' Jessica Piche Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2009 01:44 PM To histonet cc Subject [Histonet] Biocare IntelliPATH Flex Immunostainer Hi All, We are just wondering if anyone out there is using the Biocare IntelliPATH Flex Immunostainer? If so how do you like it? Any problems? How long have you had it? And any other information you could provide about it would be great. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandy.Schmitz <@t> leica-microsystems.com Tue Jul 28 16:00:32 2009 From: Sandy.Schmitz <@t> leica-microsystems.com (Sandy.Schmitz@leica-microsystems.com) Date: Tue Jul 28 16:00:43 2009 Subject: [Histonet] Schmitz, Sandy is out of the office. Message-ID: I will be out of the office starting 07/28/2009 and will not return until 08/05/2009. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cfitz <@t> telus.net Tue Jul 28 21:31:24 2009 From: cfitz <@t> telus.net (Cathy) Date: Tue Jul 28 21:31:28 2009 Subject: [Histonet] computer order enrty In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA56AB80C@ITSSSXM01V6.one.ads.che.org> References: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83374@EMAIL.archildrens.org><4A6F4858.7070605@pathology.washington.edu><001e01ca0fbd$8bb6f7a0$a324e6e0$@com> <5D64396A0D4A5346BEBC759022AAEAA56AB80C@ITSSSXM01V6.one.ads.che.org> Message-ID: <645560E763004C4CAF7D28D33CC59370@your8ba846406f> We are also looking at OE for Pathology and this would be for multiple sites. How does everyone find the specimen information provided by the ordering department and are there any fields such as clinical history that are mandatory? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, July 28, 2009 1:00 PM To: Histonet Subject: RE: [Histonet] computer order enrty Yes, Michael. Barcoding would eliminate the necessity of separating. That's an old rule/habit that many of us have because it is easier to sort out the errors if numbering gets messed up. When you are in a lab of all like specimens there is not that option, of course. So you stay up all night worrying about mixing up specimens. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Tuesday, July 28, 2009 15:56 To: 'Victor Tobias'; 'Horn, Hazel V' Cc: histonet@lists.utsouthwestern.edu; 'Hutton, Allison' Subject: RE: [Histonet] computer order enrty Victor, just as an fyi, in our system, THAT's when we create an accession number. Prior to this point, it's only an ORDER #. This gives the lab the flexibility to separate 'like' accn #'s, plus some other things. I concur that the benefits of an order entry interface are HUGE. The only caveat is that you usually have to 'massage' the surgical case data. For pap smears, order entry data is often times 100% accurate. But I'd still like to hear the 'details' about why it's commonplace to separate like specimen. I have a suspicion that this would no longer be needed if all materials are bar coded, but I need to hear the details behind this policy in order to make up my mind. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, July 28, 2009 1:50 PM To: Horn, Hazel V Cc: histonet@lists.utsouthwestern.edu; Hutton, Allison Subject: Re: [Histonet] computer order enrty I thought I'd give you my observations on OE. I don't know anything about Meditech, but with PowerPath it can be very nice. We have established OE with EPIC. When we receive the specimen, we enter the order number and virtually every field you need is filled in, patient demographics, referring physician, specimen and clinical information. Our accessioner just verifies the information, sometimes the specimen needs to be changed to pull in the correct lab orders. It is a great time saver. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Horn, Hazel V wrote: > We are hoping to do the same here. But OE does not assign a specimen > number. The specimen number would be assigned when you receive the > specimen. > > As for like specimens, we have to accession them together as we get so > many GI bx's that's it's impossible to separate them. We do use a > color code system to separate the biopsy cases. We use 6 different > alternating colors and the color is dictated in the gross description. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Hutton, Allison > Sent: Tuesday, July 28, 2009 9:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] computer order enrty > > We are currently looking into computer order entry for our pathology > specimens (using meditech). However, our major concern is that the > specimens will be assigned a surgical number when the specimen is > ordered in the OR, GI, radiology, etc and like specimens may not be > separated. Does anyone utilize the order entry module, and if so, how > do you prevent like specimens for getting back to back numbers? > Thanks in advance, > Allison > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ********************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not > the intended recipient, or an employee or agent responsible for > delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pauloafaria <@t> hotmail.com Tue Jul 28 22:34:32 2009 From: pauloafaria <@t> hotmail.com (Paulo Faria) Date: Tue Jul 28 22:34:36 2009 Subject: [Histonet] General Orientation Checklist In-Reply-To: References: Message-ID: I am also interested in this General Orientation Checklist. Thank you, Paulo Faria Rio de Janeiro - Brazil > Date: Tue, 28 Jul 2009 14:38:18 -0400 > From: ASelf@georgetownhospitalsystem.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] General Orientation Checklist > > Good Day Histonetters, > > > > Does anyone have a general orientation checklist for histology for new > hires that they would be willing to share with me? Thanks in advance for > your help, Amy > > > > > > Amy Self > > Georgetown Hospital System > > 843-527-7179 > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Show them the way! Add maps and directions to your party invites. http://www.microsoft.com/windows/windowslive/products/events.aspx From laurie.reilly <@t> jcu.edu.au Wed Jul 29 01:04:47 2009 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Wed Jul 29 01:07:48 2009 Subject: [Histonet] Minnows? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E469C2@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E469C2@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <69A9D3997841450E8FA500250C5ED247@health.ad.jcu.edu.au> Dear Sally and Histonet, Fish can be fun. If they are young the bones are soft enough to cut without decal. Or if you can fix them in an acidic fixative like Bouin's the bones will be soft enough to not give trouble. We do median sagittal sections and process and embed both sides. They look really pretty stained with your favourite trichrome. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, 28 July 2009 10:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Minnows? I've got two silvery minnows to deal with. One is about ?" and one 1". I've not done fish and am not sure if I should decal them first and try to process them whole or what! A whole mount would be interesting to look at but maybe that's not the best way. I can't imagine dissecting out tiny little organs... Anyone have any experience with tiny fish? I'd appreciate any clues! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Wed Jul 29 07:08:54 2009 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Wed Jul 29 07:09:01 2009 Subject: [Histonet] General Orientation Checklist In-Reply-To: References: Message-ID: <8910.202.125.145.178.1248869334.squirrel@brain.net.pk> The file is attached with. M. Tahseen, Supervisor Histopathology SKMCH&RC, Lahore, Pakistan > Good Day Histonetters, > > > > Does anyone have a general orientation checklist for histology for new > hires that they would be willing to share with me? Thanks in advance for > your help, Amy > > > > > > Amy Self > > Georgetown Hospital System > > 843-527-7179 > > NOTE: > The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify us immediately by replying to this message and deleting it from > your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jqb7 <@t> cdc.gov Wed Jul 29 07:12:47 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jul 29 07:14:23 2009 Subject: [Histonet] Sakura Tissue Tek Xpress In-Reply-To: <1085e7000907271218j436b5db1y60a995fee852cbe2@mail.gmail.com> References: <1085e7000907271218j436b5db1y60a995fee852cbe2@mail.gmail.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3425@LTA3VS011.ees.hhs.gov> I love our Xpress! Our lab is certainly not typical...we are very different from a routine hospital lab. We are an infectious diseases group so primarily receive liver tissue (both autopsy and biopsy), skin biopsies, kidney, lung, GI.......pretty much everything other than breast, uterus, prostate and the like. We do not work a night shift so our justification for the instrument was primarily for outbreaks, urgent cases, etc. It saves a huge amount of time when it is critical for us to get results ASAP. Our IHC are at least as good as with traditional processing and in some cases better. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Monday, July 27, 2009 3:19 PM To: histonet Subject: [Histonet] Sakura Tissue Tek Xpress My group is considering the purchase of the Sakura Tissue Tek Xpress Continuous Rapid Tissue Processor. Can any Histonetters give me feedback on their experience with this instrument? I'd like to hear the good and the bad. Can you justify the cost? How did you modify your staffing schedule? Does it produce quality results on biopsies? and on surgicals? and fatty tissue? How does it effect IHC? Feel free to reply on the Histonet, or, if you'd prefer, you may email me privately. Thank you! Daniel Schneider, MD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Wed Jul 29 08:34:21 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Jul 29 08:34:38 2009 Subject: [Histonet] RE: Beecher... In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74A7@wlmmsx01.nemours.org> I agree 100%. Beecher is the worst vendor I have ever dealt with...and unfortunately for Tissue array equipment....your choices are pretty much Beecher, Beeecher or Beecher..Chemicon had one, at a time...but, Beecher bought it from them. Isn't there some laws about monopolies? They seem to have one on tissue array. I have been wanting to purchase one, but their unresponsive nature has made warry to the point that I am considering the Sakura system (uses templates)....with some personal improvements of my own. Though it seems expensive to me (Sukura templates), at least I will have some control. Leica! Sakura! and you other guys!....Where is your competitive nature? Believe me, it wouldn't be much of a competition, because I think the working techs would like to buy a tissue arrayer from almost anyone else but Beecher....unless they change their ways. Oh, and Beecher ...please note: a good product is only as good as the service afterward. (...and it is a good product, isn't it?) Somewhere I do have a cell phone number for the head guy at Beecher. I am not on site today, so will check tomorrow. ......and all my fellow techs...I feel your "Beecher" pain! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, July 28, 2009 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Razors for dissection (Vande Berg, Mariah) 2. Re: Razors for dissection (Rene J Buesa) 3. Beecher tissue array maker (Patti Loykasek) 4. Help with Connexin 43 (Amy Porter) 5. RE: Beecher tissue array maker (McMahon, Loralee A) 6. RE: Beecher tissue array maker (Thomas Pier) 7. RE: Beecher tissue array maker (McMahon, Loralee A) 8. RE: Razors for dissection (jstaruk) 9. RE: GMS-Fungus on nails (Ingles Claire ) 10. Sakura Tissue Tek Xpress (Daniel Schneider) 11. Workshop (Lee & Peggy Wenk) 12. Re: Beecher tissue array maker (Patricia Bourne) 13. resins for embedding of PEG scaffolds (PALMER Jason (SVHM)) 14. Sakura Rapid Tissue Processor (Jean Warren) 15. Minnows? (Breeden, Sara) 16. Pathologist needed (gibiopsypath@aol.com) 17. Re: Minnows? (Kathleen Roberts) 18. Re: Sakura Rapid Tissue Processor (Daniel Schneider) 19. Fishy Notes (Breeden, Sara) 20. Re: Sakura Rapid Tissue Processor (Bryan Hewlett) 21. computer order enrty (Hutton, Allison) 22. Temperature of the dead body storage refrigerator (tahseen@brain.net.pk) 23. Re: Sakura Rapid Tissue Processor (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 27 Jul 2009 12:15:46 -0500 From: "Vande Berg, Mariah" Subject: [Histonet] Razors for dissection To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi We are looking for razor blades to be used in specimen dissection. The tissue is frozen and will be cut by hand. So the blade is like a long straight razor with a ridge along the back that is rounded. The length I believe is 2". It will be used in a research lab. If anyone has any info or descriptions please let me know. Thanks so much! Mariah Vande Berg, HT ASCP Children's Hospital of WI ------------------------------ Message: 2 Date: Mon, 27 Jul 2009 10:22:34 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Razors for dissection To: "histonet@lists.utsouthwestern.edu" , MariahVande Berg Message-ID: <657044.39161.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Some types of very similar one-edge razor can be bought at any office supplies store, cheaper than at any medical supplier. Ren? J. --- On Mon, 7/27/09, Vande Berg, Mariah wrote: From: Vande Berg, Mariah Subject: [Histonet] Razors for dissection To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 27, 2009, 1:15 PM Hi We are looking for razor blades to be used in specimen dissection.? The tissue is frozen and will be cut by hand.? So the blade is like a long straight razor with a ridge along the back that is rounded.? The length I believe is 2".? It will be used in a research lab.? If anyone has any info or descriptions please let me know.? Thanks so much! Mariah Vande Berg, HT ASCP Children's Hospital of WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 27 Jul 2009 10:33:56 -0700 From: Patti Loykasek Subject: [Histonet] Beecher tissue array maker To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 4 Date: Mon, 27 Jul 2009 13:47:57 -0400 From: "Amy Porter" Subject: [Histonet] Help with Connexin 43 To: "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Anyone out there doing FFPE IHC on rat for Connexin 43 - I am having trouble getting the titration right on this & just wondering what types of dilutions anyone is using??? I am using a standard avidin/biotin complex staining system with and endogenous AV/BI block and not getting anything of any consequence in my negative control. thanks - Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ------------------------------ Message: 5 Date: Mon, 27 Jul 2009 14:25:04 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] Beecher tissue array maker To: Patti Loykasek , histonet Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C949E2@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" I have had the incredible pleasure of trying to contact someone, anyone at beecher. It is impossible. I placed two separate orders more than 6 months ago and no one has responded to my voicemail, emails. Luckily one of the orders arrived and now we are waiting on the second order. It may be another 6 months. I have never worked with a more unresponsive company in my life. I will not purchase from them in the future. I don't recommend them to anyone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Mon 7/27/2009 1:33 PM To: histonet Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 27 Jul 2009 13:35:55 -0500 From: "Thomas Pier" Subject: RE: [Histonet] Beecher tissue array maker To: , , Message-ID: <4A6DAD3B020000DF0001A62E@gwmail.medicine.wisc.edu> Content-Type: text/plain; charset=US-ASCII I have had the same problem. It's almost as if nobody answers the phone there. They're less than 20 miles from my building, but they might as well be the Ukraine. Tom Pier >>> "McMahon, Loralee A" 07/27/09 >>> 1:31 PM >>> I have had the incredible pleasure of trying to contact someone, anyone at beecher. It is impossible. I placed two separate orders more than 6 months ago and no one has responded to my voicemail, emails. Luckily one of the orders arrived and now we are waiting on the second order. It may be another 6 months. I have never worked with a more unresponsive company in my life. I will not purchase from them in the future. I don't recommend them to anyone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Mon 7/27/2009 1:33 PM To: histonet Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 27 Jul 2009 14:43:48 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] Beecher tissue array maker To: Thomas Pier , , Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C949E5@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" Maybe you can drive by and knock on the door for us!!! Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: Thomas Pier [mailto:tp2@medicine.wisc.edu] Sent: Mon 7/27/2009 2:35 PM To: histonet@pathology.swmed.edu; ploykasek@phenopath.com; McMahon, Loralee A Subject: RE: [Histonet] Beecher tissue array maker I have had the same problem. It's almost as if nobody answers the phone there. They're less than 20 miles from my building, but they might as well be the Ukraine. Tom Pier >>> "McMahon, Loralee A" 07/27/09 >>> 1:31 PM >>> I have had the incredible pleasure of trying to contact someone, anyone at beecher. It is impossible. I placed two separate orders more than 6 months ago and no one has responded to my voicemail, emails. Luckily one of the orders arrived and now we are waiting on the second order. It may be another 6 months. I have never worked with a more unresponsive company in my life. I will not purchase from them in the future. I don't recommend them to anyone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Mon 7/27/2009 1:33 PM To: histonet Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 27 Jul 2009 15:01:38 -0400 From: "jstaruk" Subject: RE: [Histonet] Razors for dissection To: "'Vande Berg, Mariah'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Pathco offers a handle and 2' double-edged blade. The blades are the exact same as "carpet" blades found at Home Depot ($16/100). Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vande Berg, Mariah Sent: Monday, July 27, 2009 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Razors for dissection Hi We are looking for razor blades to be used in specimen dissection. The tissue is frozen and will be cut by hand. So the blade is like a long straight razor with a ridge along the back that is rounded. The length I believe is 2". It will be used in a research lab. If anyone has any info or descriptions please let me know. Thanks so much! Mariah Vande Berg, HT ASCP Children's Hospital of WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 27 Jul 2009 14:00:09 -0500 From: "Ingles Claire " Subject: RE: [Histonet] GMS-Fungus on nails To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We always run a PAS(manual) on our nails. Alot less harsh than GMS. We cut doubles and automatically stain them in case one falls off, and we always use plus slides. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Clare Thornton Sent: Fri 7/24/2009 10:30 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 27 Jul 2009 14:18:33 -0500 From: Daniel Schneider Subject: [Histonet] Sakura Tissue Tek Xpress To: histonet Message-ID: <1085e7000907271218j436b5db1y60a995fee852cbe2@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 My group is considering the purchase of the Sakura Tissue Tek Xpress Continuous Rapid Tissue Processor. Can any Histonetters give me feedback on their experience with this instrument? I'd like to hear the good and the bad. Can you justify the cost? How did you modify your staffing schedule? Does it produce quality results on biopsies? and on surgicals? and fatty tissue? How does it effect IHC? Feel free to reply on the Histonet, or, if you'd prefer, you may email me privately. Thank you! Daniel Schneider, MD ------------------------------ Message: 11 Date: Mon, 27 Jul 2009 21:35:10 -0400 From: "Lee & Peggy Wenk" Subject: [Histonet] Workshop To: Message-ID: Content-Type: text/plain; charset="us-ascii" Would like some input from Histonetters, sent directly to my home email, not through Histonet. Lpwenk@sbcglobal.net I'm giving a workshop at NSH this year on cultural and religious considerations in the laboratory, along with a minister who is in charge of our hospital's pastoral care education. He gave a 30 minute talk at our state meeting a few years ago, and everyone thought he and the information was great. Once or twice, he's talked to our MT and HT/HTL students about this topic, and we're including him again this year in our student's management talks. NSH has accepted this as a 3 hour workshop. We've been putting together information about different religons and cultures, in relation to laboratories (blood transfusions, transplants, autopsies, genetics, fetuses, placentas, burial, etc.). We're going to concentrate on what we as laboratorians can do to respect others' beliefs and traditions. If you have an example of some religious or cultural consideration that you or your lab have done in the past, and how you handled it, would you mind passing it along to my home email? Michigan has a lot of people from all over the world, and our hospital and lab have tried to become aware of the needs of our patients and their families in terms of religious and cultural wishes. But Michigan may not have a high enough population for our hospital/lab to have experience with all cultures, nationalities, religions, etc. For example, I should imagine the US states on the west coast have more experience with people from the Polynesian Islands than we do. ANative American traditions differ by location. And I'd love histonetters from other countries to chime in also. We know we can't cover every single religion or nationality, and we can't cover the spectrum of differences within each religion and culture. But we're going to cover the main considerations, and how the laboratories can accommodate our patients. We're going to cover the typical Anatomic Pathology labs (histology, autopsy) but will also cover some Clinical Pathology labs (phlebotomy, blood transfusions, genetics, etc.), so that participants can take the document back to their place of employment, and start a dialog with all the labs. Thanks in advance for your help. Peggy Wenk Lpwenk@sbcglobal.net ------------------------------ Message: 12 Date: Mon, 27 Jul 2009 21:06:34 -0700 (PDT) From: Patricia Bourne Subject: Re: [Histonet] Beecher tissue array maker To: Patti Loykasek Cc: HistoNet Server Message-ID: <67731.79146.qm@web51703.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This company is a very small group who do not have the support staff to answer the phones, emails etc.? However, they are very much in demand.? I was always successful dealing with them by leaving a voice mail and letting them know that my order was urgent.? I would fax my orders directly and the product usually came once I started this way of ordering.? They are most likely over-whelmed with the success of their idea.? I also double ordered to avoid running out of TMA needles.? ________________________________ From: Patti Loykasek To: histonet Sent: Monday, July 27, 2009 10:33:56 AM Subject: [Histonet] Beecher tissue array maker Hi All. I have been attempting to contact Beecher Instruments regarding an ETA on TMA needles. So far, no replies to voice mail or email. Does anyone have a personal contact at this company? Thanks. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 28 Jul 2009 15:25:50 +1000 From: "PALMER Jason (SVHM)" Subject: [Histonet] resins for embedding of PEG scaffolds To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all. I am looking for a non-paraffin embedding medium for some rat tissues which have grown into a polyethylene glycol-based porous tissue engineering scaffold (a second component of the scaffold is PCL, polycaprolactone). Being PEG-based, the scaffolds are essentially hydrophilic in nature. Standard paraffin embedding gives sections which are somewhat crumbly and which adhere very poorly to slides for both routine and immunostaining, whether they are coated with APES, poly-L-lysine or chrome gelatin. Hence I wish to try something else. Have tried Technovit 8100, ie a glycol methacrylate, but this led to unacceptable distortion / diffusion of the scaffold material, something which we do not see with paraffin. I don't have much experience with the different resins, but was wondering if one of the epoxy media - hydrophobic rather than the hydrophilic methacrylates - might be worth a go? We want to do light microscopy, not EM, on the samples - at least H&E's or toluidine blue, but immunostaining would be great too. Any thoughts appreciated. Thanks a bunch! Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 14 Date: Tue, 28 Jul 2009 08:20:54 -0400 From: "Jean Warren" Subject: [Histonet] Sakura Rapid Tissue Processor To: "histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have the Sakura rapid tissue processor at my hospital lab, which is a large private hospital. We have had it three years and it has been somewhat of a disappointment:: We have been told that you should not process breasts in it, because you will not get reliable results for FISH. Its implementation has created schedule changes that have caused some good techs to leave. The scenario to get a case out the same day is rare. If a patient has surgery at 7 am and we receive it by 8 am, it is accessioned and grossed in. Except for biopsies, the specimen still will need 2 hours fixation in formalin. When we receive it in Histology at 1030 am, it must go in pre-processing solution for 30 minutes. At 1100, we process it for approx 1 hour. Then embed, cut and stain. Our docs would get it well after lunch and, if all is OK, they can get the report out. And, there are not many cases that meet that time criterion. One other drawback is that it is more labor intensive to handle 10 blocks ten times a day than to handle 100 at one time. Our lab processes from 400-750 blocks a day and less than 100 a day are processed on that processor. All we are handling on the instrument are bxs, bone marrows and cytology blocks. If too large a specimen is placed on it, we usually have a problem. Endometrial bxs, skins and cones have not had favorable results. On the other hand, biopsies, especially livers, look and cut better. Our hematology expert wants all bone marrows done that way. We also rapid process most cytology that way, but bloody cases still need formalin fixation. It is much simpler to change the processor ( and more expensive ). We tried to do most specimens on it with terrible failure. If anyone gets it, I would recommend starting slowly with a few specimen types and gradually adding. Our hope is that it will be more useful in the future. I know, rather wordy answer! ------------------------------ Message: 15 Date: Tue, 28 Jul 2009 06:36:03 -0600 From: "Breeden, Sara" Subject: [Histonet] Minnows? To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E469C2@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="iso-8859-1" I've got two silvery minnows to deal with. One is about ?" and one 1". I've not done fish and am not sure if I should decal them first and try to process them whole or what! A whole mount would be interesting to look at but maybe that's not the best way. I can't imagine dissecting out tiny little organs... Anyone have any experience with tiny fish? I'd appreciate any clues! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 16 Date: Tue, 28 Jul 2009 09:09:35 -0400 From: gibiopsypath@aol.com Subject: [Histonet] Pathologist needed To: histonet@lists.utsouthwestern.edu Message-ID: <8CBDD9BCA3BBDF6-D1C-487@webmail-dh36.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" A private, well established, Ohio private pathology lab is looking for a pathologist for a busy GI practice.? The salary is excellent and comes with a highly efficient lab team.? For more information send a CV to this email address.? All inquiries will be kept confidential.? ------------------------------ Message: 17 Date: Tue, 28 Jul 2009 10:09:50 -0400 From: Kathleen Roberts Subject: Re: [Histonet] Minnows? To: "Breeden, Sara" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4A6F06AE.60300@rci.rutgers.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I get everything from mice to birds to fish (zebrafish, but close enough for our purposes), with the occasional larger animal thrown in (deer, horse). Since I'm in academic research, things are different for me-I receive instructions on what to do with every tissue sample submitted, so if I were you, I would first check with the person/organization that sent them and ask them what they want. Likely you will have to decal them, process them whole, and cut them to the level that they want. Good luck! Kathleen Roberts Principal Lab Technician Neurotoxicology Laboratories Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 (732) 445-6914 FAX (732) 445-6905 Breeden, Sara wrote: >I've got two silvery minnows to deal with. One is about ?" and one 1". I've not done fish and am not sure if I should decal them first and try to process them whole or what! A whole mount would be interesting to look at but maybe that's not the best way. I can't imagine dissecting out tiny little organs... Anyone have any experience with tiny fish? I'd appreciate any clues! > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 18 Date: Tue, 28 Jul 2009 09:01:36 -0500 From: Daniel Schneider Subject: Re: [Histonet] Sakura Rapid Tissue Processor To: Jean Warren Cc: histonet Message-ID: <1085e7000907280701p5da3924bg7be9c39ded659bb@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Actually, that was perfect, wordy replies are what I had hoped for. How is the automated embedding working out for you? The way I see things, automated embedding is the true killer feature of the Xpress -- that is, if it works. Why don't skins process well on the Xpress? (When I first appreciated the automated embedding feature, my first thought was "Great, no more misembedded pigmented skin lesions!" But if we can't do skins, well...) Breasts cancers require at least 8 hours formalin fixation for reliable Her2Neu FISH; presumably that's why you've been told not to do breasts on the Xpress. That said, is there a reason why we couldn't or shouldn't process breast core Bx's on the Xpress provided they've been sitting overnight in formalin? Thanks!!! Daniel Schneider On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren wrote: > We have the Sakura rapid tissue processor at my hospital lab, which is a > large private hospital. We have had it three years and it has been somewhat > of a disappointment:: > > We have been told that you should not process breasts in it, because you > will not get reliable > results for FISH. > > Its implementation has created schedule changes that have caused some good > techs to leave. > > The scenario to get a case out the same day is rare. > If a patient has surgery at 7 am and we receive it by 8 am, it is > accessioned > and grossed in. > Except for biopsies, the specimen still will need 2 hours fixation in > formalin. When > we receive it in Histology at 1030 am, it must go in pre-processing > solution > for 30 minutes. At 1100, we process it for approx 1 hour. > Then embed, cut and stain. Our docs would get it well after lunch and, if > all is > OK, they can get the report out. > And, there are not many cases that meet that time criterion. > > One other drawback is that it is more labor intensive to handle 10 blocks > ten times a day than to handle 100 at one time. Our lab processes from > 400-750 blocks a day and less than 100 a day are processed on that > processor. All we are handling on the instrument are bxs, bone marrows and > cytology blocks. If too large a specimen is placed on it, we usually have a > problem. Endometrial bxs, skins and cones have not had favorable results. > > On the other hand, biopsies, especially livers, look and cut better. Our > hematology expert wants all bone marrows done that way. We also rapid > process most cytology that way, but bloody cases still need formalin > fixation. > > It is much simpler to change the processor ( and more expensive ). > > We tried to do most specimens on it with terrible failure. If anyone gets > it, I > would recommend starting slowly with a few specimen types and gradually > adding. > > Our hope is that it will be more useful in the future. > > I know, rather wordy answer! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 19 Date: Tue, 28 Jul 2009 08:17:33 -0600 From: "Breeden, Sara" Subject: [Histonet] Fishy Notes To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E469CF@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" Thank you to everyone that responded to my Fishy Predicament! I have lots of hints, clues, procedures and background on how to cut these little silvery minnows and I appreciate every single one of you for responding. In cases like this, I choose to go directly to the Histo Experts and I'm always steered in the right direction. So - thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 20 Date: Tue, 28 Jul 2009 10:36:19 -0400 From: "Bryan Hewlett" Subject: Re: [Histonet] Sakura Rapid Tissue Processor To: "Daniel Schneider" , "Jean Warren" Cc: histonet Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Daniel, You wrote; >>That said, is there a reason why we couldn't or shouldn't > process breast core Bx's on the Xpress provided they've been sitting > overnight in formalin? See following article. Concensus Recomendations on ER testing in breast cancer by IHC(Appl Immunohistochem Mol Morphol 2008;16:513-520) Bryan ---- Original Message ----- From: "Daniel Schneider" To: "Jean Warren" Cc: "histonet" Sent: Tuesday, July 28, 2009 10:01 AM Subject: Re: [Histonet] Sakura Rapid Tissue Processor > Actually, that was perfect, wordy replies are what I had hoped for. > > How is the automated embedding working out for you? The way I see things, > automated embedding is the true killer feature of the Xpress -- that is, > if > it works. > > Why don't skins process well on the Xpress? (When I first appreciated the > automated embedding feature, my first thought was "Great, no more > misembedded pigmented skin lesions!" But if we can't do skins, well...) > > Breasts cancers require at least 8 hours formalin fixation for reliable > Her2Neu FISH; presumably that's why you've been told not to do breasts on > the Xpress. That said, is there a reason why we couldn't or shouldn't > process breast core Bx's on the Xpress provided they've been sitting > overnight in formalin? > Thanks!!! > Daniel Schneider > On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren > wrote: > >> We have the Sakura rapid tissue processor at my hospital lab, which is a >> large private hospital. We have had it three years and it has been >> somewhat >> of a disappointment:: >> >> We have been told that you should not process breasts in it, because you >> will not get reliable >> results for FISH. >> >> Its implementation has created schedule changes that have caused some >> good >> techs to leave. >> >> The scenario to get a case out the same day is rare. >> If a patient has surgery at 7 am and we receive it by 8 am, it is >> accessioned >> and grossed in. >> Except for biopsies, the specimen still will need 2 hours fixation in >> formalin. When >> we receive it in Histology at 1030 am, it must go in pre-processing >> solution >> for 30 minutes. At 1100, we process it for approx 1 hour. >> Then embed, cut and stain. Our docs would get it well after lunch and, if >> all is >> OK, they can get the report out. >> And, there are not many cases that meet that time criterion. >> >> One other drawback is that it is more labor intensive to handle 10 blocks >> ten times a day than to handle 100 at one time. Our lab processes from >> 400-750 blocks a day and less than 100 a day are processed on that >> processor. All we are handling on the instrument are bxs, bone marrows >> and >> cytology blocks. If too large a specimen is placed on it, we usually >> have a >> problem. Endometrial bxs, skins and cones have not had favorable results. >> >> On the other hand, biopsies, especially livers, look and cut better. Our >> hematology expert wants all bone marrows done that way. We also rapid >> process most cytology that way, but bloody cases still need formalin >> fixation. >> >> It is much simpler to change the processor ( and more expensive ). >> >> We tried to do most specimens on it with terrible failure. If anyone gets >> it, I >> would recommend starting slowly with a few specimen types and gradually >> adding. >> >> Our hope is that it will be more useful in the future. >> >> I know, rather wordy answer! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 21 Date: Tue, 28 Jul 2009 10:41:04 -0400 From: "Hutton, Allison" Subject: [Histonet] computer order enrty To: Message-ID: <38A56C4F4630D348A50B3720409270870744FE1C@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" We are currently looking into computer order entry for our pathology specimens (using meditech). However, our major concern is that the specimens will be assigned a surgical number when the specimen is ordered in the OR, GI, radiology, etc and like specimens may not be separated. Does anyone utilize the order entry module, and if so, how do you prevent like specimens for getting back to back numbers? Thanks in advance, Allison ------------------------------ Message: 22 Date: Tue, 28 Jul 2009 20:57:49 +0600 (PKST) From: tahseen@brain.net.pk Subject: [Histonet] Temperature of the dead body storage refrigerator To: histonet@lists.utsouthwestern.edu Message-ID: <22066.202.125.145.178.1248793069.squirrel@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 Dear all, The temperature of the dead body storage refrigerator was fluctuating up to 6 and 7 centigrade. I am looking referances if any ? Thanks advance Muhammad Tahseen Histology Supervisor SKMCH&RC LAHORE,PAKISTAN ------------------------------ Message: 23 Date: Tue, 28 Jul 2009 08:03:20 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Sakura Rapid Tissue Processor To: Jean Warren , Daniel Schneider Cc: histonet Message-ID: <930240.62689.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Daniel: The other part of your question refers to costs. Under separate cover I am sending you an article I wrote on the cost effectiveness of the Xpress and other MW technology tissue processor (although the Xpress is an hybrid). Please also pay attention to the previous answer where it is stated that the schedules changes (which can be "drastic") caused the lost of some good technicians. Many things have to be considered when such a change is planned. Ren? J. --- On Tue, 7/28/09, Daniel Schneider wrote: From: Daniel Schneider Subject: Re: [Histonet] Sakura Rapid Tissue Processor To: "Jean Warren" Cc: "histonet" Date: Tuesday, July 28, 2009, 10:01 AM Actually, that was perfect, wordy replies are what I had hoped for. How is the automated embedding working out for you?? The way I see things, automated embedding is the true killer feature of the Xpress -- that is, if it works. Why don't skins process well on the Xpress?? (When I first appreciated the automated embedding feature, my first thought was "Great, no more misembedded pigmented skin lesions!"? But if we can't do skins, well...) Breasts cancers require at least 8 hours formalin fixation for reliable Her2Neu FISH; presumably that's why you've been told not to do breasts on the Xpress.? That said, is there a reason why we couldn't or shouldn't process breast core Bx's on the Xpress provided they've been sitting overnight in formalin? Thanks!!! Daniel Schneider On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren wrote: > We have the Sakura rapid tissue processor at my hospital lab, which is a > large private hospital. We have had it three years and it has been somewhat > of a disappointment:: > > We have been told that you should not process breasts in it, because you > will not get reliable > results for FISH. > > Its implementation has created schedule changes that have caused some good > techs to leave. > > The scenario to get a case out the same day is rare. > If a patient has surgery at 7 am and we receive it by 8 am, it is > accessioned > and grossed in. > Except for biopsies, the specimen still will need 2 hours fixation in > formalin. When > we receive it in Histology at 1030 am, it must go in pre-processing > solution > for 30 minutes. At 1100, we process it for approx 1 hour. > Then embed, cut and stain. Our docs would get it well after lunch and, if > all is > OK, they can get the report out. > And, there are not many cases that meet that time criterion. > > One other drawback is that it is more labor intensive to handle 10 blocks > ten times a day than to handle 100 at one time. Our lab processes from > 400-750 blocks a day and less than 100 a day are processed on that > processor. All we are handling on the instrument are bxs, bone marrows and > cytology blocks. If too large a specimen is placed on it,? we usually have a > problem. Endometrial bxs, skins and cones have not had favorable results. > > On the other hand, biopsies, especially livers, look and cut better. Our > hematology expert wants all bone marrows done that way. We also rapid > process most cytology that way, but bloody cases still need formalin > fixation. > > It is much simpler to change the processor ( and more expensive ). > > We tried to do most specimens on it with terrible failure. If anyone gets > it, I > would recommend starting slowly with a few specimen types and gradually > adding. > > Our hope is that it will be more useful in the future. > > I know, rather wordy answer! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 35 **************************************** From SAllen <@t> exchange.hsc.mb.ca Wed Jul 29 09:27:24 2009 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Jul 29 09:27:33 2009 Subject: [Histonet] Artifact in Muscle bx's frozen for extended time Message-ID: Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From Kristopher.Kalleberg <@t> unilever.com Wed Jul 29 09:31:39 2009 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Wed Jul 29 09:31:44 2009 Subject: [Histonet] flash frozen tissue Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg From jo-ann.bader <@t> mcgill.ca Wed Jul 29 10:38:05 2009 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Wed Jul 29 10:41:20 2009 Subject: [Histonet] Mouse Eyes Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 From MITCHELLJA <@t> email.chop.edu Wed Jul 29 10:42:27 2009 From: MITCHELLJA <@t> email.chop.edu (Janice Mitchell) Date: Wed Jul 29 10:42:49 2009 Subject: [Histonet] SLIDE PRINTER Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice From rjbuesa <@t> yahoo.com Wed Jul 29 11:26:48 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 29 11:26:52 2009 Subject: [Histonet] Mouse Eyes In-Reply-To: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Jul 29 12:04:46 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jul 29 12:04:53 2009 Subject: [Histonet] SLIDE PRINTER Message-ID: <57BE698966D5C54EAE8612E8941D76830628C3C1@EXCHANGE3.huntingtonhospital.com> We have two units that we bought from Accuplace when it was called the PSLIM. We had a fair amount of problems with them and I was ready to return them, but Accuplace sent someone out to service them, and they are working really well. I've heard that when Fisher took over the slide printer, they "revamped" it, so that it would have less problems. We have the Thermo Fisher Printmate cassette labeler, and we have a 2D barcode print on the cassette. The PSLIM (Slide Mate) reads the barcode and prints out the number of slides that we tell it to. We have it programmed very simply right now, but it is flexible and you are able to have it print various ways. I love it! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mitchell Sent: Wednesday, July 29, 2009 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SLIDE PRINTER Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WBENTON <@t> umm.edu Wed Jul 29 12:27:12 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Wed Jul 29 12:27:40 2009 Subject: [Histonet] 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) In-Reply-To: References: Message-ID: <4A704E30.D886.00F4.3@umm.edu> Sharon, We process quite a few samples as well and I did them at Hopkins with a similar time frame for the old tissue. We did not experience the problems you are referencing when we pulled the cases out from storage. What is the exact type of artifact that you are seeing? Is it freeze/thawing artifact or is it the tiny holes that come from water crystals? I could send you our freezing technique if you like. I would also suggest using cryo vials that have rubber gaskets which are designed for use in those extreme temperatures. Walter Benton Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Tina.Hayes <@t> va.gov Wed Jul 29 12:54:46 2009 From: Tina.Hayes <@t> va.gov (Hayes, Tina J.) Date: Wed Jul 29 12:54:56 2009 Subject: [Histonet] urine cytology with red blood cells Message-ID: <01A34750423B874399B8AF6674D90A270420A87D@VHAV01MSGA1.v01.med.va.gov> Our pathologist would like us to set up a procedure for cytologies (urines, in particular) that will NOT lyse red blood cells. Does anybody know of a transport/preservation medium for urine cytologies that does not lyse RBC's? And is anybody using it with ThinPrep? We are currently using ThinPrep with Cytolyt, however the Cytolyt solution is designed to lyse the RBC's. From contact <@t> excaliburpathology.com Wed Jul 29 13:49:54 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Jul 29 13:49:59 2009 Subject: [Histonet] Mouse Eyes In-Reply-To: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> References: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Message-ID: <213548.97351.qm@web1113.biz.mail.sk1.yahoo.com> Do they also want undetached retina? If so, do not use NBF to fix. Paula Pierce, BA, AAS, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Ave. Moore, OK 73160 405-759-3953 Specializing in whole eye histology. All species. ? ________________________________ From: "Jo-Ann Bader, Ms." To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, July 29, 2009 10:38:05 AM Subject: [Histonet] Mouse Eyes We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Wed Jul 29 14:09:19 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Jul 29 14:09:34 2009 Subject: [Histonet] RE: Artifact in long stored muscle bx In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B2@wlmmsx01.nemours.org> Have also been doing them since 1982....know what you mean about the artifact. We store in Bell-Art containers (availavle thru Fisher)..We have found no others to have the ability to storewithout cracking for so long. We do place about 100 ul of water in the storage container...and place it in the LN for a quick freeze before we place any sample in the container, to hydrate over long period of time. Some other hint's...we use 7-8% trangacanth gum for mounting samples....and place it only at the base of the mounted tissue. Once tissue is down into the medium....you will always have artifact. Note also: if you are snap freezing in isopentane, be sure to allow the isopentane to completely evaporate from the same (in a cryo)until it appears dry, before placing in the container. Isopentane left on the samples causes the same sort of artifact. This are my best suggestions....I wold love to hear any others though....always nice to learn more. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** From histoman3000 <@t> live.com Wed Jul 29 14:13:03 2009 From: histoman3000 <@t> live.com (Jimmy Markum) Date: Wed Jul 29 14:13:07 2009 Subject: [Histonet] SLIDE PRINTER In-Reply-To: <4A7035A3020000000052CB35@email.chop.edu> References: <4A7035A3020000000052CB35@email.chop.edu> Message-ID: Janice, I know this well! It is a terrible item. This was originally sold under AccuPlace and it barely worked, but showed very nice at various meetings under a very controlled environment. In fact it worked so well, that the nice people at Thermo decided to purchase this from AccuPlace and more than double the price and hope to dupe us to purchase at almost 2700% increase for the same item. While I have returned my units plenty of time and Thermo has added some of their "crack" engineering to the item. Save your money and run from Thermo, buy a Leica or wait for TBS or someone else to come up with something better for a cost you can justify. I will never purchase anything from Thermo again, every time you do, it only justifies what they are doing to the market. Jimmy > Date: Wed, 29 Jul 2009 11:42:27 -0400 > From: MITCHELLJA@email.chop.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] SLIDE PRINTER > > Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live? SkyDrive?: Store, access, and share your photos. See how. http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009 From cbarone <@t> NEMOURS.ORG Wed Jul 29 14:17:28 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Jul 29 14:17:48 2009 Subject: [Histonet] RE:Muscle Artifact In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B4@wlmmsx01.nemours.org> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** From cbarone <@t> NEMOURS.ORG Wed Jul 29 14:19:11 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Jul 29 14:19:24 2009 Subject: [Histonet] RE: Muscle Artifact In-Reply-To: References: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B5@wlmmsx01.nemours.org> Containers: Fisher # NC9283918 $32.90/6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** From valerio2 <@t> buffalo.edu Wed Jul 29 14:22:01 2009 From: valerio2 <@t> buffalo.edu (Mike Valerio) Date: Wed Jul 29 14:22:05 2009 Subject: [Histonet] Problems with IHC Message-ID: <61151.1248895321@buffalo.edu> Hello, I am having problems staining my FFPE mouse brains for macrophages. I started out with F4/80 marker, but have now moved to CD68. My positive controls (mouse spleen and liver) work fine, but I cannot get anything to stain in my sample tissues. I have see through H and E staining that macrophages are present, could anyone help me with this problem? Mike Valerio University of Buffalo From CIngles <@t> uwhealth.org Wed Jul 29 14:17:39 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jul 29 14:22:41 2009 Subject: [Histonet] Artifact in Muscle bx's frozen for extended time References: Message-ID: We do everything you do, except we surround the tissue with oct in the tube before freezing. Otherwise, it's freezer burn city. (in TX I think) :) We also just write the info on the side of the bottle with a thin sharpie instead of the cork thing. We usually store several tubes from the same case as we don't know how much tissue they will want in the future. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon Allen Sent: Wed 7/29/2009 9:27 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Artifact in Muscle bx's frozen for extended time Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JPeters <@t> bostwicklaboratories.com Wed Jul 29 14:26:27 2009 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Wed Jul 29 14:27:35 2009 Subject: [Histonet] Glycophorin A antibody Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F064FB71C@mail1.BOSTWICK.COM> Has anyone had any luck with Glycophorin A antibody (Cell Marque) used on paraffin-embedded (Formical decalcified) bone marrow biopsies? Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com From CIngles <@t> uwhealth.org Wed Jul 29 14:30:00 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jul 29 14:30:04 2009 Subject: [Histonet] flash frozen tissue References: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Message-ID: We are a Mohs clinic and I had done a study on the feasability of IHC on frozen skin. I cut slides as normal then fixed in acetone before using Biocare's Mach 3 kit (Alk. phos. with Red chromogen) with predilute antibodies. They mostly turned out well, except for the S100. I don't believe that one turns out satisfactory in frozen tissue no matter what you do. I did a MART-1 stain, HMB-45 (predilute from Innovex), and Tyrosinase. There was very little background. Biocare will be more than happy to help with any questions you might have. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kalleberg, Kristopher Sent: Wed 7/29/2009 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] flash frozen tissue Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Wed Jul 29 14:31:20 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Wed Jul 29 14:32:27 2009 Subject: [Histonet] RE: Muscle Artifact In-Reply-To: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B5@wlmmsx01.nemours.org> References: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B5@wlmmsx01.nemours.org> Message-ID: This number did not work for me at the Fisher website. Is it current? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Wednesday, July 29, 2009 2:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Muscle Artifact Containers: Fisher # NC9283918 $32.90/6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Jul 29 14:56:14 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jul 29 14:56:08 2009 Subject: [Histonet] slide and cassette labelers Message-ID: <23735D5B905B49A69DA0245AA96B4026@Patsyoffice> Does anyone have any experience with the Leica IPC cassette labeler and IPS slide labeler or the Sukura Tissue Tek Auto-write systems. We are setting up a new lab and need labelers that will interface with windows operating systems and do not have these experiences. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From eric.halpern <@t> comphealth.com Wed Jul 29 15:03:08 2009 From: eric.halpern <@t> comphealth.com (eric.halpern@comphealth.com) Date: Wed Jul 29 15:03:16 2009 Subject: [Histonet] Eric M. Halpern/CompHealth is out of the office. Message-ID: I will be out of the office starting 07/29/2009 and will not return until 08/03/2009. I will out of the office on Vacation starting Monday 7/29 and will return back to work on Monday 8/3 in my absence if you need immediated attention please contact Kiersten Ferreira the Operations Manager at 866-782-9029 ext 5842 or at kiersten.ferreira@comphealth.com. Regards, Eric From algranth <@t> email.arizona.edu Wed Jul 29 15:36:47 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Jul 29 15:37:16 2009 Subject: [Histonet] SLIDE PRINTER In-Reply-To: References: <4A7035A3020000000052CB35@email.chop.edu> Message-ID: BUT...I have a small lab and can't justify a more expensive printer so we purchased this printer - in fact we probably purchased the last one that AccuPlace sold before Thermo got it. I like it a lot and even though we have returned a couple of them we have no complaints. It does a nice job. We were lucky to get it at the AccuPlace price. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Jul 29, 2009, at 12:13 PM, Jimmy Markum wrote: > > Janice, > I know this well! It is a terrible item. This was originally sold > under AccuPlace and it barely worked, but showed very nice at > various meetings under a very controlled environment. In fact it > worked so well, that the nice people at Thermo decided to purchase > this from AccuPlace and more than double the price and hope to dupe > us to purchase at almost 2700% increase for the same item. > While I have returned my units plenty of time and Thermo has added > some of their "crack" engineering to the item. > Save your money and run from Thermo, buy a Leica or wait for TBS or > someone else to come up with something better for a cost you can > justify. > I will never purchase anything from Thermo again, every time you do, > it only justifies what they are doing to the market. > Jimmy > > >> Date: Wed, 29 Jul 2009 11:42:27 -0400 >> From: MITCHELLJA@email.chop.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] SLIDE PRINTER >> >> Anyone out there in Histoland have any experience with Thermo slide >> mate? We are looking into purchasing a few. Thanks for any >> input, Janice >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Windows Live? SkyDrive?: Store, access, and share your photos. See > how. > http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ndevans <@t> stanford.edu Wed Jul 29 16:05:29 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Jul 29 16:05:33 2009 Subject: [HISTONET] Antigen retrieval paraffin skin samples Message-ID: <002c01ca1090$4d32f280$ce1d41ab@DellDesktop2> Dear all, Might anyone be able to offer their advice on the best method to retrieve antigens on paraffin embedded skin sections? I am immunostaining for epithelial cytokeratins, and I have block-to-block variation in the quality of the staining - some block give consistently good staining while others giving negligible staining. The tissues were prepared by fixation in 4% PFA for 24 - 48 hours followed by dehydration and embedding. I currently use 15 mins Ficin digestion. I suspect that the differences may be accounted for by variability in the length of time samples were fixed in PFA and then 70% ethanol. Best wishes Nick From njoydobro <@t> aol.com Wed Jul 29 19:15:00 2009 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Wed Jul 29 19:15:18 2009 Subject: [Histonet] urine cytology with red blood cells In-Reply-To: <01A34750423B874399B8AF6674D90A270420A87D@VHAV01MSGA1.v01.med.va.gov> References: <01A34750423B874399B8AF6674D90A270420A87D@VHAV01MSGA1.v01.med.va.gov> Message-ID: <8CBDEC1E9D0309D-12B8-BA1@webmail-mh11.sysops.aol.com> try this: BD CytoRich? Blue Preservative* General Purpose Cell Preservative. Excellent for Urine and non-hemolytic samples. Recommended for use as a general cytology preservative Gene -----Original Message----- From: Hayes, Tina J. To: histonet@lists.utsouthwestern.edu Sent: Wed, Jul 29, 2009 1:54 pm Subject: [Histonet] urine cytology with red blood cells Our pathologist would like us to set up a procedure for cytologies urines, in particular) that will NOT lyse red blood cells. Does nybody know of a transport/preservation medium for urine cytologies hat does not lyse RBC's? And is anybody using it with ThinPrep? We re currently using ThinPrep with Cytolyt, however the Cytolyt solution s designed to lyse the RBC's. _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Jul 29 20:27:33 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 29 20:27:46 2009 Subject: [Histonet] Artifact in Muscle bx's frozen for extended time In-Reply-To: Message-ID: Sharon, Your answer is probably in the following article in the Journal of Histotechnology (Volume 32, number 2, June 2009): "Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology" Authors: Iren Horkayne-Szakaly, Glenn D. Sandberg, Joren Keylock, Elisabeth J. Rushing Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 30 July 2009 12:27 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Artifact in Muscle bx's frozen for extended time Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mike <@t> pathview.com Wed Jul 29 08:18:49 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Jul 29 21:05:07 2009 Subject: [Histonet] SLIDE PRINTER In-Reply-To: References: <4A7035A3020000000052CB35@email.chop.edu> Message-ID: <005101ca104f$214d3810$63e7a830$@com> I guess I'm confused. If pricing is an issue, why not go with a Zebra printer that prints labels. I wholeheartedly agree that printing directly on slides is a preferred solution, but the up front cost is a killer. I wouldn't blame a lab for going with slide LABELS. Frankly, if it was me, I'd save the money on this printer and put it towards a cassette labeler. If money is not an issue, then I'd get both. P.S. ..and you guys are getting me nervous. This is the second person that I've heard that has 'returned a printer'. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, July 29, 2009 3:37 PM Cc: HISTONET Subject: Re: [Histonet] SLIDE PRINTER BUT...I have a small lab and can't justify a more expensive printer so we purchased this printer - in fact we probably purchased the last one that AccuPlace sold before Thermo got it. I like it a lot and even though we have returned a couple of them we have no complaints. It does a nice job. We were lucky to get it at the AccuPlace price. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Jul 29, 2009, at 12:13 PM, Jimmy Markum wrote: > > Janice, > I know this well! It is a terrible item. This was originally sold > under AccuPlace and it barely worked, but showed very nice at > various meetings under a very controlled environment. In fact it > worked so well, that the nice people at Thermo decided to purchase > this from AccuPlace and more than double the price and hope to dupe > us to purchase at almost 2700% increase for the same item. > While I have returned my units plenty of time and Thermo has added > some of their "crack" engineering to the item. > Save your money and run from Thermo, buy a Leica or wait for TBS or > someone else to come up with something better for a cost you can > justify. > I will never purchase anything from Thermo again, every time you do, > it only justifies what they are doing to the market. > Jimmy > > >> Date: Wed, 29 Jul 2009 11:42:27 -0400 >> From: MITCHELLJA@email.chop.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] SLIDE PRINTER >> >> Anyone out there in Histoland have any experience with Thermo slide >> mate? We are looking into purchasing a few. Thanks for any >> input, Janice >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Windows LiveT SkyDriveT: Store, access, and share your photos. See > how. > http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lgarratt <@t> ichr.uwa.edu.au Wed Jul 29 21:26:48 2009 From: lgarratt <@t> ichr.uwa.edu.au (Luke Garratt) Date: Wed Jul 29 21:27:02 2009 Subject: [Histonet] IHC on stored BALf cytospins Message-ID: <994C9D8C9339DD4A95F9466889E3243403E4EC80@TWEETY.ichr.uwa.edu.au> Hello all, We have a large resource of unfixed cytospun glass slides of human bronchoalveolar lavage fluid (BALf) that we would like to use for immunocytochemistry. For each BAL we have one pair of slides that are air-dried and then stored at RT, these date back up to 2001. We also have pairs of slides that were directly frozen at -20?C dating back to early 2006. Although we have generally used one of the air-dried slides for differential counts with Leishman's stain, the other 3 have remained in storage. Having searched the literature for advice on how long a slide can be stored before it is used for ICC, to no avail, can somebody please give us some guidance on a) which slide is best to use (dried or frozen), b) the upper limit of storage, c) any special treatment the older slides may need and d) any recommended literature. Many thanks, Luke Garratt Research Assistant Australian Respiratory Early Surveillance Team for Cystic Fibrosis Telethon Institute for Child Health Research P.O. Box 855, West Perth, Western Australia, 6872 T +61 8 9489 7819 F +61 8 9489 7700 W www.arestcf.org E lgarratt@ichr.uwa.edu.au ##################################################################################### This e-mail message has been scanned for Viruses and Content and cleared by MailMarshal ##################################################################################### From cbarone <@t> NEMOURS.ORG Thu Jul 30 08:45:45 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Jul 30 08:46:00 2009 Subject: [Histonet] RE: regarding muscle bx containers containers In-Reply-To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74C0@wlmmsx01.nemours.org> Received confirmation on correct number from Fisher on muscle storage containers: However.... here is reply from staff... "Carol I called Fisher this morning and had them check; it is the correct number and is on the website. They may however have to order directly from Fisher. Terry" (McLaughlin,Histo...Core Lab) Hope this helps...the containers make a big difference in dehydration/or not.....We use only this type of container for storing muscle bx's for histochem(along with the frozen water droplet inside). If it is surface freeze thaw artifact...try to cut a little past with a face cut, or two, in taking your first cut... and remember not to cut anything that is down in the freezing medium. It will always have artifact. If the sample is "cracking"...you may be keeping it too long in the isopenatane at the initial snap-freeze...Muscle bx's are a creature of their own. Let me know if this helps! Lots of luck!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 9:28 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: SLIDE PRINTER (Laurie Colbert) 2. 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) (Walter Benton) 3. urine cytology with red blood cells (Hayes, Tina J.) 4. Re: Mouse Eyes (Paula Pierce) 5. RE: Artifact in long stored muscle bx (Barone, Carol ) 6. RE: SLIDE PRINTER (Jimmy Markum) 7. RE:Muscle Artifact (Barone, Carol ) 8. RE: Muscle Artifact (Barone, Carol ) 9. Problems with IHC (Mike Valerio) 10. RE: Artifact in Muscle bx's frozen for extended time (Ingles Claire ) 11. Glycophorin A antibody (Justin Peters) 12. RE: flash frozen tissue (Ingles Claire ) 13. RE: RE: Muscle Artifact (Walters, Katherine S) 14. slide and cassette labelers (Patsy Ruegg) 15. Eric M. Halpern/CompHealth is out of the office. (eric.halpern@comphealth.com) 16. Re: SLIDE PRINTER (Andrea Grantham) 17. Antigen retrieval paraffin skin samples (Nicholas David Evans) 18. Re: urine cytology with red blood cells (njoydobro@aol.com) 19. RE: Artifact in Muscle bx's frozen for extended time (Tony Henwood) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 10:04:46 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] SLIDE PRINTER To: "Janice Mitchell" , Message-ID: <57BE698966D5C54EAE8612E8941D76830628C3C1@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" We have two units that we bought from Accuplace when it was called the PSLIM. We had a fair amount of problems with them and I was ready to return them, but Accuplace sent someone out to service them, and they are working really well. I've heard that when Fisher took over the slide printer, they "revamped" it, so that it would have less problems. We have the Thermo Fisher Printmate cassette labeler, and we have a 2D barcode print on the cassette. The PSLIM (Slide Mate) reads the barcode and prints out the number of slides that we tell it to. We have it programmed very simply right now, but it is flexible and you are able to have it print various ways. I love it! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mitchell Sent: Wednesday, July 29, 2009 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SLIDE PRINTER Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 13:27:12 -0400 From: "Walter Benton" Subject: [Histonet] 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) To: Message-ID: <4A704E30.D886.00F4.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Sharon, We process quite a few samples as well and I did them at Hopkins with a similar time frame for the old tissue. We did not experience the problems you are referencing when we pulled the cases out from storage. What is the exact type of artifact that you are seeing? Is it freeze/thawing artifact or is it the tiny holes that come from water crystals? I could send you our freezing technique if you like. I would also suggest using cryo vials that have rubber gaskets which are designed for use in those extreme temperatures. Walter Benton Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 13:54:46 -0400 From: "Hayes, Tina J." Subject: [Histonet] urine cytology with red blood cells To: Message-ID: <01A34750423B874399B8AF6674D90A270420A87D@VHAV01MSGA1.v01.med.va.gov> Content-Type: text/plain; charset="us-ascii" Our pathologist would like us to set up a procedure for cytologies (urines, in particular) that will NOT lyse red blood cells. Does anybody know of a transport/preservation medium for urine cytologies that does not lyse RBC's? And is anybody using it with ThinPrep? We are currently using ThinPrep with Cytolyt, however the Cytolyt solution is designed to lyse the RBC's. ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:49:54 -0700 (PDT) From: Paula Pierce Subject: Re: [Histonet] Mouse Eyes To: "Jo-Ann Bader, Ms." , Histonet Message-ID: <213548.97351.qm@web1113.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Do they also want undetached retina? If so, do not use NBF to fix. Paula Pierce, BA, AAS, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Ave. Moore, OK 73160 405-759-3953 Specializing in whole eye histology. All species. ? ________________________________ From: "Jo-Ann Bader, Ms." To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, July 29, 2009 10:38:05 AM Subject: [Histonet] Mouse Eyes We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 15:09:19 -0400 From: "Barone, Carol " Subject: [Histonet] RE: Artifact in long stored muscle bx To: histonet@lists.utsouthwestern.edu Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B2@wlmmsx01.nemours.org> Content-Type: text/plain; charset=iso-8859-1 Have also been doing them since 1982....know what you mean about the artifact. We store in Bell-Art containers (availavle thru Fisher)..We have found no others to have the ability to storewithout cracking for so long. We do place about 100 ul of water in the storage container...and place it in the LN for a quick freeze before we place any sample in the container, to hydrate over long period of time. Some other hint's...we use 7-8% trangacanth gum for mounting samples....and place it only at the base of the mounted tissue. Once tissue is down into the medium....you will always have artifact. Note also: if you are snap freezing in isopentane, be sure to allow the isopentane to completely evaporate from the same (in a cryo)until it appears dry, before placing in the container. Isopentane left on the samples causes the same sort of artifact. This are my best suggestions....I wold love to hear any others though....always nice to learn more. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** ------------------------------ Message: 6 Date: Wed, 29 Jul 2009 15:13:03 -0400 From: Jimmy Markum Subject: RE: [Histonet] SLIDE PRINTER To: , Message-ID: Content-Type: text/plain; charset="Windows-1252" Janice, I know this well! It is a terrible item. This was originally sold under AccuPlace and it barely worked, but showed very nice at various meetings under a very controlled environment. In fact it worked so well, that the nice people at Thermo decided to purchase this from AccuPlace and more than double the price and hope to dupe us to purchase at almost 2700% increase for the same item. While I have returned my units plenty of time and Thermo has added some of their "crack" engineering to the item. Save your money and run from Thermo, buy a Leica or wait for TBS or someone else to come up with something better for a cost you can justify. I will never purchase anything from Thermo again, every time you do, it only justifies what they are doing to the market. Jimmy > Date: Wed, 29 Jul 2009 11:42:27 -0400 > From: MITCHELLJA@email.chop.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] SLIDE PRINTER > > Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live(tm) SkyDrive(tm): Store, access, and share your photos. See how. http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009 ------------------------------ Message: 7 Date: Wed, 29 Jul 2009 15:17:28 -0400 From: "Barone, Carol " Subject: [Histonet] RE:Muscle Artifact To: histonet@lists.utsouthwestern.edu Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B4@wlmmsx01.nemours.org> Content-Type: text/plain; charset=iso-8859-1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** ------------------------------ Message: 8 Date: Wed, 29 Jul 2009 15:19:11 -0400 From: "Barone, Carol " Subject: [Histonet] RE: Muscle Artifact To: histonet@lists.utsouthwestern.edu Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A74B5@wlmmsx01.nemours.org> Content-Type: text/plain; charset=iso-8859-1 Containers: Fisher # NC9283918 $32.90/6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** ------------------------------ Message: 9 Date: Wed, 29 Jul 2009 15:22:01 -0400 From: Mike Valerio Subject: [Histonet] Problems with IHC To: Message-ID: <61151.1248895321@buffalo.edu> Content-Type: text/plain; charset="utf-8" Hello, I am having problems staining my FFPE mouse brains for macrophages. I started out with F4/80 marker, but have now moved to CD68. My positive controls (mouse spleen and liver) work fine, but I cannot get anything to stain in my sample tissues. I have see through H and E staining that macrophages are present, could anyone help me with this problem? Mike Valerio University of Buffalo ------------------------------ Message: 10 Date: Wed, 29 Jul 2009 14:17:39 -0500 From: "Ingles Claire " Subject: RE: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We do everything you do, except we surround the tissue with oct in the tube before freezing. Otherwise, it's freezer burn city. (in TX I think) :) We also just write the info on the side of the bottle with a thin sharpie instead of the cork thing. We usually store several tubes from the same case as we don't know how much tissue they will want in the future. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon Allen Sent: Wed 7/29/2009 9:27 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Artifact in Muscle bx's frozen for extended time Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 29 Jul 2009 15:26:27 -0400 From: "Justin Peters" Subject: [Histonet] Glycophorin A antibody To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F064FB71C@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Has anyone had any luck with Glycophorin A antibody (Cell Marque) used on paraffin-embedded (Formical decalcified) bone marrow biopsies? Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com ------------------------------ Message: 12 Date: Wed, 29 Jul 2009 14:30:00 -0500 From: "Ingles Claire " Subject: RE: [Histonet] flash frozen tissue To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are a Mohs clinic and I had done a study on the feasability of IHC on frozen skin. I cut slides as normal then fixed in acetone before using Biocare's Mach 3 kit (Alk. phos. with Red chromogen) with predilute antibodies. They mostly turned out well, except for the S100. I don't believe that one turns out satisfactory in frozen tissue no matter what you do. I did a MART-1 stain, HMB-45 (predilute from Innovex), and Tyrosinase. There was very little background. Biocare will be more than happy to help with any questions you might have. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kalleberg, Kristopher Sent: Wed 7/29/2009 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] flash frozen tissue Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 29 Jul 2009 14:31:20 -0500 From: "Walters, Katherine S" Subject: RE: [Histonet] RE: Muscle Artifact To: "Barone, Carol " , Message-ID: Content-Type: text/plain; charset="iso-8859-1" This number did not work for me at the Fisher website. Is it current? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Wednesday, July 29, 2009 2:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Muscle Artifact Containers: Fisher # NC9283918 $32.90/6. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: "Sharon Allen" Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: "Kalleberg, Kristopher" Subject: [Histonet] flash frozen tissue To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C067F7868@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg ------------------------------ Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Message-ID: <76D119EF12C904418800ED67CCB2062905E2AB9071@EXMBXVS1.campus.mcgill.ca> Content-Type: text/plain; charset="us-ascii" We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 ------------------------------ Message: 4 Date: Wed, 29 Jul 2009 11:42:27 -0400 From: "Janice Mitchell" Subject: [Histonet] SLIDE PRINTER To: Message-ID: <4A7035A3020000000052CB35@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Anyone out there in Histoland have any experience with Thermo slide mate? We are looking into purchasing a few. Thanks for any input, Janice ------------------------------ Message: 5 Date: Wed, 29 Jul 2009 09:26:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" , " Ms.Jo-Ann Bader" Message-ID: <950812.3007.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. Ren? J. --- On Wed, 7/29/09, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes.? The lens must remain intact.? What is the? best method to process, embed and cut them?? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 38 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 29 Jul 2009 13:56:14 -0600 From: "Patsy Ruegg" Subject: [Histonet] slide and cassette labelers To: "'Histonet'" Message-ID: <23735D5B905B49A69DA0245AA96B4026@Patsyoffice> Content-Type: text/plain; charset="us-ascii" Does anyone have any experience with the Leica IPC cassette labeler and IPS slide labeler or the Sukura Tissue Tek Auto-write systems. We are setting up a new lab and need labelers that will interface with windows operating systems and do not have these experiences. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 15 Date: Wed, 29 Jul 2009 16:03:08 -0400 From: eric.halpern@comphealth.com Subject: [Histonet] Eric M. Halpern/CompHealth is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/29/2009 and will not return until 08/03/2009. I will out of the office on Vacation starting Monday 7/29 and will return back to work on Monday 8/3 in my absence if you need immediated attention please contact Kiersten Ferreira the Operations Manager at 866-782-9029 ext 5842 or at kiersten.ferreira@comphealth.com. Regards, Eric ------------------------------ Message: 16 Date: Wed, 29 Jul 2009 13:36:47 -0700 From: Andrea Grantham Subject: Re: [Histonet] SLIDE PRINTER Cc: HISTONET Message-ID: Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes BUT...I have a small lab and can't justify a more expensive printer so we purchased this printer - in fact we probably purchased the last one that AccuPlace sold before Thermo got it. I like it a lot and even though we have returned a couple of them we have no complaints. It does a nice job. We were lucky to get it at the AccuPlace price. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Jul 29, 2009, at 12:13 PM, Jimmy Markum wrote: > > Janice, > I know this well! It is a terrible item. This was originally sold > under AccuPlace and it barely worked, but showed very nice at > various meetings under a very controlled environment. In fact it > worked so well, that the nice people at Thermo decided to purchase > this from AccuPlace and more than double the price and hope to dupe > us to purchase at almost 2700% increase for the same item. > While I have returned my units plenty of time and Thermo has added > some of their "crack" engineering to the item. > Save your money and run from Thermo, buy a Leica or wait for TBS or > someone else to come up with something better for a cost you can > justify. > I will never purchase anything from Thermo again, every time you do, > it only justifies what they are doing to the market. > Jimmy > > >> Date: Wed, 29 Jul 2009 11:42:27 -0400 >> From: MITCHELLJA@email.chop.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] SLIDE PRINTER >> >> Anyone out there in Histoland have any experience with Thermo slide >> mate? We are looking into purchasing a few. Thanks for any >> input, Janice >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Windows Live(tm) SkyDrive(tm): Store, access, and share your photos. See > how. > http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 17 Date: Wed, 29 Jul 2009 14:05:29 -0700 (PDT) From: "Nicholas David Evans" Subject: [HISTONET] Antigen retrieval paraffin skin samples To: Message-ID: <002c01ca1090$4d32f280$ce1d41ab@DellDesktop2> Content-Type: text/plain; charset="US-ASCII" Dear all, Might anyone be able to offer their advice on the best method to retrieve antigens on paraffin embedded skin sections? I am immunostaining for epithelial cytokeratins, and I have block-to-block variation in the quality of the staining - some block give consistently good staining while others giving negligible staining. The tissues were prepared by fixation in 4% PFA for 24 - 48 hours followed by dehydration and embedding. I currently use 15 mins Ficin digestion. I suspect that the differences may be accounted for by variability in the length of time samples were fixed in PFA and then 70% ethanol. Best wishes Nick ------------------------------ Message: 18 Date: Wed, 29 Jul 2009 20:15:00 -0400 From: njoydobro@aol.com Subject: Re: [Histonet] urine cytology with red blood cells To: Tina.Hayes@va.gov, Histonet@lists.utsouthwestern.edu Message-ID: <8CBDEC1E9D0309D-12B8-BA1@webmail-mh11.sysops.aol.com> Content-Type: text/plain; charset="utf-8" try this: BD CytoRich?"? Blue Preservative* General Purpose Cell Preservative. Excellent for Urine and non-hemolytic samples. Recommended for use as a general cytology preservative Gene -----Original Message----- From: Hayes, Tina J. To: histonet@lists.utsouthwestern.edu Sent: Wed, Jul 29, 2009 1:54 pm Subject: [Histonet] urine cytology with red blood cells Our pathologist would like us to set up a procedure for cytologies urines, in particular) that will NOT lyse red blood cells. Does nybody know of a transport/preservation medium for urine cytologies hat does not lyse RBC's? And is anybody using it with ThinPrep? We re currently using ThinPrep with Cytolyt, however the Cytolyt solution s designed to lyse the RBC's. _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 30 Jul 2009 11:27:33 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Artifact in Muscle bx's frozen for extended time To: "Sharon Allen" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sharon, Your answer is probably in the following article in the Journal of Histotechnology (Volume 32, number 2, June 2009): "Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology" Authors: Iren Horkayne-Szakaly, Glenn D. Sandberg, Joren Keylock, Elisabeth J. Rushing Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 30 July 2009 12:27 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Artifact in Muscle bx's frozen for extended time Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70?C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 39 **************************************** From decker <@t> HEALTHCARESCOUTS.COM Thu Jul 30 08:53:40 2009 From: decker <@t> HEALTHCARESCOUTS.COM (Michael Decker) Date: Thu Jul 30 09:08:58 2009 Subject: [Histonet] Histotechnician Opportunities Message-ID: Good morning, My name is Michael Decker, the National Director of Recruitment with Healthcare Scouts (www.healthcarescouts.com ) and I'm currently working on a number of positions for Histotechnologists across the United States. If you are interested in any of them, please email me at decker@healthcarescouts.com or call me any time at 1.800.708.0605 x134 or on my cell phone at 281.744.2672. Thank you for your time and I look forward to hearing from anyone looking for a great new opportunity. Here are the positions we currently have open: GROSSING TECHNICIAN - Westchester, NY (Day and Night Shifts available) POSITION PURPOSE Perform duties that relate to the histology specimen grossing of surgically removed tissue under the supervision of the histology and/or laboratory supervisor. ESSENTIAL FUNCTIONS AND BASIC DUTIES * Ensure specimen integrity upon arrival. * Accession and enter the case in TPS * Dictate a gross description for each biopsy specimen, dissect and prepare tissue for cassettes. * Gross small skin biopsies and un-oriented excisions with minimal supervision. Larger and oriented skin excisions (under the supervision of a supervisor or pathologist). * Prepare dissected tissue specimens and place into cassettes. * Load prepared cassettes in Tissue Processors. * Operation of tissue processors * Familiar with safety procedures and standard operating procedures. * Compliance with all Federal and State regulations pertaining to histology: * Adhere to workload requirements. * Maintenance of accurate work records (i.e., Grossing Station filter maintenance logs, QA Logs, etc.). * Able to evaluate new laboratory equipment and procedures. * Able to perform Quality Control and Quality Assurance Procedures. * Working knowledge and understanding of TPS System. * Assist in the professional development of laboratory aides. * Daily housekeeping. EDUCATION / CERTIFICATION: Associate Degree or BS in Science or related Field. NY State Licensed / Permit or eligible. REQUIRED KNOWLEDGE: Knowledgeable in the handling of specimens from accessioning to final specimen storage. Working knowledge of laboratory organization, operational procedures, Quality Control, Quality Assurance, and laboratory safety. Working knowledge and understanding of computers. EXPERIENCE REQUIRED: Hired with experience; must possess one - three year's experience HISTOTECHNICIAN - Philadelphia, PA (Day Shift) POSITION PURPOSE Histo-Technician with at least one-two years full-time experience in histology under the general supervision of the histology supervisor, render assistance in the day-to-day administration of the laboratory. ESSENTIAL FUNCTIONS AND BASIC DUTIES * Knowledgeable in the handling of specimens from grossing to final specimen storage. * Dictate a gross description for each biopsy specimen, dissect and prepare tissue for cassettes. * Gross small skin/tissue biopsies and un-oriented excisions with minimal supervision. Larger and oriented skin excisions (under the supervision of a supervisor or pathologist). * Load prepared cassettes in Tissue Processors. * Operation and maintenance of the embedding station * Assist supervisor with day to day administration of Histology Laboratory. Defined duties include: * Staff development and training. * Assisting in the administration of all Quality Control/Quality Assurance Programs. * Assisting in the scheduling of work flow. * Compliance with all Federal and State regulations pertaining to histology. Specifically: * Adhere to workload requirements. * Maintenance of accurate work records (i.e., Maintenance Logs, Temperature Logs, etc.) * State inspection. * Timely completion of instrument specific QC documentation when applicable * Evaluates new equipment and procedures. * Reviews slides for overall quality of preparation such as staining intensity, proper cover slipping and stain artifacts to help insure accurate diagnosis. * Timely distribution of completed patient materials to assigned pathologists. * Daily maintenance of cleanliness and neatness of Histology Lab and instrumentation in all aspects. * Perform other duties as directed by supervisor. EDUCATION / CERTIFICATION: * Associates Degree or BS Degree in science or related field from a regionally accredited college/university plus successful completion of a NAACLS accredited Histotechnology program. REQUIRED KNOWLEDGE: * Knowledge of all department policies and procedures, familiar with State and Federal regulations governing laboratories. EXPERIENCE REQUIRED: * Minimum 1-2 Histology Technician experience. SKILLS / ABILITIES: * Strong interpersonal skills, exceptional organizational skills, supervisory skills. Must be able to work with hazardous chemicals. Working knowledge of computers and programs. Ability to type 55 wpm. Good clerical skills. HISTOLOGY SUPERVISOR - Washington State (Day Shift) Full-Time, M-F Qualifications: HT or HTL certification and four years prior experience. Familiarity with CLIA and CAP guidelines and the ability to effectively manage people. This position plans, coordinates and directs all Histology support activities including scheduling, oversight of daily work, QA activities, staff training and development of new technologies. Preference will be given to those candidates with previous supervisory experience and Lean/Six Sigma knowledge. Our client offers competitive salaries and an outstanding benefits package including medical, dental, life and disability insurance, 401(k), and a liberal paid time off program. Relocation assistance is available. HISTOTECHNOLOGIST - Northern Los Angeles, CA 1-3 years experience as a Histotechnician AA or AS degree or equivalent training and experience HT (ASCP) or HTL (ASCP) HISTOLOGY SUPERVISOR - Northern Los Angeles, CA Five or more years of experience in the field of histotechnology. Up-to-date knowledge of newest histology equipment/techniques. Experience in setting up a new histology laboratory. Best Regards, Michael Decker National Director of Sales and Recruitment Healthcare Scouts 2269 Lee Road, Suite 200 Winter Park, Florida 32789 Office: 800.708.0605 x134 Cell: 281.744.2672 Fax: 877.478.6064 www.healthcarescouts.com From Ronald.Houston <@t> nationwidechildrens.org Thu Jul 30 09:22:29 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jul 30 09:23:23 2009 Subject: [Histonet] IgG subclass 4 Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645A05@chi2k3ms01.columbuschildrens.net> Any know where I can get an antibody to IgG4 that works on FFPE sections? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From judith_pardue <@t> memorial.org Thu Jul 30 10:03:58 2009 From: judith_pardue <@t> memorial.org (Pardue, Judith) Date: Thu Jul 30 10:04:30 2009 Subject: [Histonet] Negative Pressure Rooms Message-ID: Our histology lab is negative pressure and the hospital is remolding and wants the department to use one door to enter and exit the department.This will mean we will have to do that via and outside breezeway. Our concern is will this cause problems with our staining due to humidity or moisture getting in our solutions. Judith Pardue HT (ASCP) QIHC Memorial Healthcare System Chattanooga, Tn. 37343 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rjbuesa <@t> yahoo.com Thu Jul 30 10:26:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 30 10:26:50 2009 Subject: [Histonet] Negative Pressure Rooms In-Reply-To: Message-ID: <493308.15041.qm@web65701.mail.ac4.yahoo.com> Not very likely. Please remember that our?trade/art as a whole is conducted in Tropical countries as well, some of which do not have the "luxuries" of the A/C and the procedures are completed as expected. Ren? J. --- On Thu, 7/30/09, Pardue, Judith wrote: From: Pardue, Judith Subject: [Histonet] Negative Pressure Rooms To: histonet@lists.utsouthwestern.edu Date: Thursday, July 30, 2009, 11:03 AM Our histology lab is negative pressure and the hospital is remolding and wants the department to use one door to enter and exit the department.This will mean we will have to do that via and outside breezeway. Our concern is will this cause problems with our staining due to humidity or moisture getting in our solutions. Judith Pardue? HT (ASCP) QIHC Memorial Healthcare System Chattanooga, Tn. 37343 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential.? If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited.? If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JonSorenson <@t> chiwest.com Thu Jul 30 10:30:37 2009 From: JonSorenson <@t> chiwest.com (Sorenson, Jon (Nampa)) Date: Thu Jul 30 10:30:48 2009 Subject: [Histonet] RE:Antigen retrieval paraffin skin samples In-Reply-To: <20090730013158.13E1F57SS8@email4.catholichealth.net> References: <20090730013158.13E1F57SS8@email4.catholichealth.net> Message-ID: We use and like the PASCAL pressure cooker system from DAKO, it is uniform, convenient and fairly Easy to use. Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsorenso@chiwest.com 208-463-5267 Message: 17 Date: Wed, 29 Jul 2009 14:05:29 -0700 (PDT) From: "Nicholas David Evans" Subject: [HISTONET] Antigen retrieval paraffin skin samples To: Message-ID: <002c01ca1090$4d32f280$ce1d41ab@DellDesktop2> Content-Type: text/plain; charset="US-ASCII" Dear all, Might anyone be able to offer their advice on the best method to retrieve antigens on paraffin embedded skin sections? I am immunostaining for epithelial cytokeratins, and I have block-to-block variation in the quality of the staining - some block give consistently good staining while others giving negligible staining. The tissues were prepared by fixation in 4% PFA for 24 - 48 hours followed by dehydration and embedding. I currently use 15 mins Ficin digestion. I suspect that the differences may be accounted for by variability in the length of time samples were fixed in PFA and then 70% ethanol. Best wishes Nick From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jul 30 11:08:07 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jul 30 11:09:37 2009 Subject: [Histonet] Negative Pressure Rooms References: Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C94A06@e2k3ms1.urmc-sh.rochester.edu> Isn't there a rule about having two exits for safety depending on the square footage of the room. CAP rule maybe or it could be our fire safety people. I can't remember which. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pardue, Judith Sent: Thu 7/30/2009 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Pressure Rooms Our histology lab is negative pressure and the hospital is remolding and wants the department to use one door to enter and exit the department.This will mean we will have to do that via and outside breezeway. Our concern is will this cause problems with our staining due to humidity or moisture getting in our solutions. Judith Pardue HT (ASCP) QIHC Memorial Healthcare System Chattanooga, Tn. 37343 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From austi013 <@t> mc.duke.edu Wed Jul 29 19:05:22 2009 From: austi013 <@t> mc.duke.edu (Alan D Austin) Date: Thu Jul 30 11:57:05 2009 Subject: [Histonet] BAF 47 Message-ID: Hello Sally, I work at Duke Medical Center and we are staining our Brain tumors with BAF 47. The stain is being performed in our Immunopathology Lab by James (Jim) Burchette. His lab number is (919) 684-2058. He may be about to help you with this stain. The slides are reviewed by Doctor Roger McLendon our neuropathologist here at Duke. Good Luck..... From: "Drew Sally A." Good afternoon! We have a neuropathologist wanting to know where we can send some slides to get BAF 47 (INI-1 gene product) immunostaining done. I guess this is for an atypical teratoid/rhabdoid tumor? If someone out there does this immunostain, please contact me with the information on how to send the slides and how much the charge will be. Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sincerely, Alan Alan Austin Molecular Technologist II, Molecular Pathology Duke University Health System Room 4087 Duke South Yellow Zone Durham, NC 27710 South Office Phone:(919)684-0737 South Fax (919)684-6187 email:austi013@mc.duke.edu From jerry.santiago <@t> jax.ufl.edu Thu Jul 30 13:31:13 2009 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Thu Jul 30 13:31:19 2009 Subject: [Histonet] K-Ras by Immuno Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF30148F4BD@jaxmail.umc.ufl.edu> Anyone performing K-Ras by immunohistochemistry? If so, please contact me privately, I have some questions to ask. Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Jacksonville, FL Tel: 904-244-6149 E-mal: jerry.santiago@jax.ufl.edu From relia1 <@t> earthlink.net Thu Jul 30 14:32:33 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jul 30 14:32:37 2009 Subject: [Histonet] RELIA Histology Supervisor Job Alert - Histology Supervisor needed in Spokane, WA - Brand New Lab Message-ID: Hi Histonetters, I hope everyone is having a great day. I am currently assisting a client located in Spokane, WA with their search for a histology supervisor. My client is looking for someone with 3-5 years of experience supervising a histology lab. This is a hands on position and requires a combination of bench and administrative work. My client offers an excellent salary, great benefits and the oportunity to work in a brand new lab facility. If you or anyone you know migt be interested in more information please contact me-Pam Barker e-mail relia1@earthlink.net or toll free at 866-607-3542 I also have a supervisory position in Los Angeles and a lead tech position in San Antonio. Thanks-Pam . Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From ploykasek <@t> phenopath.com Thu Jul 30 14:56:30 2009 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Jul 30 14:56:39 2009 Subject: [Histonet] Position open in Seattle Message-ID: Due to recent growth and new projects, we have a position open in Seattle. Here is the announcement: HISTOTECHNOLOGIST PhenoPath Laboratories, PLLC, a pathology reference laboratory, has an opening for a Histotechnologist (full-time, M-F.) We are in a state-of-the-art facility located on the scenic Ship Canal in Seattle, Washington. Primary Responsibilities Responsibilities include performing routine manual and automated tests in a clinical and research histology laboratory. Under general supervision, perform routine laboratory tests such as processing, embedding, microtomy, frozen sections, H&E, special stains, routine IHC, etc. Required Skills/Experience Requires ASCP certification (or certification-eligible) Histotechnologists. PhenoPath Laboratories is committed to hiring the best person for the job. PhenoPath Laboratories is a national specialty pathology laboratory committed to providing outstanding clinical and research services. We offer an excellent compensation and benefits package, including 401k. Salary will be determined based on the overall skills and background of the chosen applicant. To Apply Please Contact: PhenoPath Laboratories, PLLC 551 N. 34th St., Suite 100 Seattle, WA 98103 Phone: 206 374-9000 Fax: 206 374-9009 E-mail: jobs@phenopath.com Please no phone calls about this position from candidates or recruiters. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From gaushil <@t> gmail.com Thu Jul 30 15:36:54 2009 From: gaushil <@t> gmail.com (shilpa shah) Date: Thu Jul 30 15:36:59 2009 Subject: [Histonet] (no subject) Message-ID: We use vegetable(food) steamer for skin. It gives consistent result. I know it is very old fashion, But its work fine. I heard dclocking chamber from Biocare gives good result too. Shilpa Shilpa Shah Muhlbauer Dermato pathology lab Pittsford NY From Rick.Garnhart <@t> memorialhealthsystem.com Thu Jul 30 15:55:25 2009 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Thu Jul 30 15:55:31 2009 Subject: [Histonet] re: Pslims In-Reply-To: Message-ID: We have 5 Pslims from Accuplace, one at each of our cutting stations. We are interface with PowerPath with AMP. We do struggle with 2 of them but it usually comes down to a network issue. We have worked extensively with Accuplace and have made great progress with resolving our issues. Let it be said that they are still issues.We are 2D bar-coded through out our system, specimen containers, cassettes and slides. We produce no cassettes or slides until they are ready to be used. We have eliminated placing tissue in the wrong numbered cassettes and picking up tissue on the wrong slide, 100% positive patient identification. We wand the cassette and as we are cutting our section the Pslim is printing our slide. Our Pslims work between 90-100% of the time, none of our cutters have to wait for the slide printer to finish to pick up the section. The slides are waiting for us to use them. We are producing between 450 and 600 slides every morning between 0600 and 0830. I would not go back to mass producing slides either hand written or printed by a large slide printer. Individual slide printing at each cutting station should be the wave of the future. If any one would like to speak directly with me please feel free to call after 1000 Mountain time M-F. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From sfeher <@t> CMC-NH.ORG Thu Jul 30 16:12:54 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Jul 30 16:20:55 2009 Subject: [Histonet] New Lab HTL Opening Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1CE2@exchange.cmc-nh.org> Hi Everyone, I have an opening for a Histotechnologist position in New Hampshire. This is a new pathology lab with all new equipment and facilities. The person filling this position will have the opportunity to be a major influence the procedures and processes that the lab will use. The start date is October 1. Between October 1 and Feb 2010, this person will be writing protocols and be my primary resource for setting up the Histology and Gross areas of the lab. Requirements are: Experience is preferred Be the primary technical resource for histology and participate in the performance of routine and specialized technical histology functions as needed to provide high quality microscopic slides of tissue for the detection of disease, to include immunohistochemistry procedures and procurement and processing of pathology specimen material. QUALIFICATIONS: Education: Bachelor's degree and HTL (ASCP) certification preferred; OR has met the minimal education and experience requirements as outlined by the American Society for Clinical Pathology (ASCP) and has HTL (ASCP) certification. Experience: Five (5) years full-time experience as a certified Histotechnologist (HTL) in a clinical laboratory setting. Apply online at: http://www.catholicmedicalcenter.org/ Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From cls71877 <@t> sbcglobal.net Thu Jul 30 22:46:16 2009 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Thu Jul 30 22:46:20 2009 Subject: [Histonet] General Orientation Checklist Message-ID: <904292.11323.qm@web81203.mail.mud.yahoo.com> Hello, I am sorry, I am also interested in this, but I don't see the attachment? Thanks, Cristi Stephenson MSA, HT(ASCP) --- On Wed, 7/29/09, tahseen@brain.net.pk wrote: From: tahseen@brain.net.pk Subject: Re: [Histonet] General Orientation Checklist To: "Amy Self" Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, July 29, 2009, 8:08 AM The file is attached with. M. Tahseen, Supervisor Histopathology SKMCH&RC, Lahore, Pakistan > Good Day Histonetters, > > > > Does anyone have a general orientation checklist for histology for new > hires that they would be willing to share with me? Thanks in advance for > your help, Amy > > > > > > Amy Self > > Georgetown Hospital System > > 843-527-7179 > > NOTE: >? The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify us immediately by replying to this message and deleting it from > your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreas.kappeler <@t> pathology.unibe.ch Fri Jul 31 00:11:22 2009 From: andreas.kappeler <@t> pathology.unibe.ch (Kappeler Andi) Date: Fri Jul 31 00:11:56 2009 Subject: AW: [Histonet] IgG subclass 4 In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21B645A05@chi2k3ms01.columbuschildrens.net> References: <979FF5962E234F45B06CF0DB7C1AABB21B645A05@chi2k3ms01.columbuschildrens.net> Message-ID: <001801ca119d$5854a380$17955c82@pi23> Hi Ronnie we use IgG4, clone HP6025, Calbiochem CatNo 411492; enzymatic pretreatment, working conc. 1 ug/ml . Works nicely. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Houston, Ronald Gesendet: Donnerstag, 30. Juli 2009 16:22 An: ihcrg@googlegroups.com; Histonet Betreff: [Histonet] IgG subclass 4 Wichtigkeit: Hoch Any know where I can get an antibody to IgG4 that works on FFPE sections? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tiina.Vesterinen <@t> hus.fi Fri Jul 31 00:56:02 2009 From: Tiina.Vesterinen <@t> hus.fi (Vesterinen Tiina) Date: Fri Jul 31 00:56:12 2009 Subject: [Histonet] Beecher MTA-1 connected to MTABooster from Alphelys Message-ID: <2C0982D85C07A34598393F0B2B6F96754138868215@vanhusmbx06.hus.fi> Hi all, I?m having some problems using MTA-1 connected to MTABooster. Especially zero point setting seems to be hard. Also the booster informs me sometimes that it?s x-axis has reached a limit point even if the there is a lot of space in the donor block and the array was made with TMADesigner. Does anyone else have same kind of problems? Tiina Vesterinen Project Researcher Institute for Molecular Medicine Finland FIMM Helsinki, Finland From mpatrick00 <@t> hotmail.com Fri Jul 31 02:14:22 2009 From: mpatrick00 <@t> hotmail.com (Michael Patrick) Date: Fri Jul 31 02:14:38 2009 Subject: [Histonet] IHC free floating sections Message-ID: I would like to stain free floating sections (fixed rat brain; 50um). Is it necessary to transfer sections to "clean" culture wells with a paint brush every time I wash or incubate with antibodies? I find this causes significant damage to my tissue (especially since I do a lot of washes etc.). Could I simply use a pipette to draw out the old solution and then replace with the appropriate solution? What do most people do in these situations? Much thanks Mike _________________________________________________________________ Stay in the loop and chat with friends, right from your inbox! http://go.microsoft.com/?linkid=9671354 From akemiat3377 <@t> yahoo.com Fri Jul 31 07:45:18 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jul 31 07:45:23 2009 Subject: [Histonet] General Orientation Checklist In-Reply-To: <904292.11323.qm@web81203.mail.mud.yahoo.com> Message-ID: <107542.58671.qm@web31304.mail.mud.yahoo.com> I too am interested in the information. ?Please send it to me. ?I tried to send information ?with an attachment in the past to histonet and it never went through. ?I guess the only way an attachment can be sent is to an individual. ?Links are acceptable. Regards,Akemi Akemi Allison-Tacha, BS, HT/HTLE-Mail: akemiat3377@yahoo.com --- On Thu, 7/30/09, Cristi stephenson wrote: From: Cristi stephenson Subject: Re: [Histonet] General Orientation Checklist To: "Amy Self" , tahseen@brain.net.pk Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 30, 2009, 8:46 PM Hello, I am sorry, I am also interested in this, but I don't see the attachment? Thanks, Cristi Stephenson MSA, HT(ASCP) --- On Wed, 7/29/09, tahseen@brain.net.pk wrote: From: tahseen@brain.net.pk Subject: Re: [Histonet] General Orientation Checklist To: "Amy Self" Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, July 29, 2009, 8:08 AM The file is attached with. M. Tahseen, Supervisor Histopathology SKMCH&RC, Lahore, Pakistan > Good Day Histonetters, > > > > Does anyone have a general orientation checklist for histology for new > hires that they would be willing to share with me? Thanks in advance for > your help, Amy > > > > > > Amy Self > > Georgetown Hospital System > > 843-527-7179 > > NOTE: >? The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify us immediately by replying to this message and deleting it from > your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Fri Jul 31 07:55:23 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Jul 31 07:55:54 2009 Subject: [Histonet] General Orientation Checklist In-Reply-To: <107542.58671.qm@web31304.mail.mud.yahoo.com> References: <904292.11323.qm@web81203.mail.mud.yahoo.com> <107542.58671.qm@web31304.mail.mud.yahoo.com> Message-ID: I am also interested. Could someone please send it to me? :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, July 31, 2009 7:45 AM To: Amy Self; tahseen@brain.net.pk; Cristi stephenson Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] General Orientation Checklist I too am interested in the information. ?Please send it to me. ?I tried to send information ?with an attachment in the past to histonet and it never went through. ?I guess the only way an attachment can be sent is to an individual. ?Links are acceptable. Regards,Akemi Akemi Allison-Tacha, BS, HT/HTLE-Mail: akemiat3377@yahoo.com --- On Thu, 7/30/09, Cristi stephenson wrote: From: Cristi stephenson Subject: Re: [Histonet] General Orientation Checklist To: "Amy Self" , tahseen@brain.net.pk Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 30, 2009, 8:46 PM Hello, I am sorry, I am also interested in this, but I don't see the attachment? Thanks, Cristi Stephenson MSA, HT(ASCP) --- On Wed, 7/29/09, tahseen@brain.net.pk wrote: From: tahseen@brain.net.pk Subject: Re: [Histonet] General Orientation Checklist To: "Amy Self" Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, July 29, 2009, 8:08 AM The file is attached with. M. Tahseen, Supervisor Histopathology SKMCH&RC, Lahore, Pakistan > Good Day Histonetters, > > > > Does anyone have a general orientation checklist for histology for new > hires that they would be willing to share with me? Thanks in advance for > your help, Amy > > > > > > Amy Self > > Georgetown Hospital System > > 843-527-7179 > > NOTE: >? The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify us immediately by replying to this message and deleting it from > your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 31 07:58:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 31 07:58:42 2009 Subject: [Histonet] IHC free floating sections In-Reply-To: Message-ID: <402925.69567.qm@web65716.mail.ac4.yahoo.com> You can do it, I have done it, BUT you will have to wash very?well the well (no pun intended) to make sure that there are no cross-reactions. Ren? J. --- On Fri, 7/31/09, Michael Patrick wrote: From: Michael Patrick Subject: [Histonet] IHC free floating sections To: histonet@lists.utsouthwestern.edu Date: Friday, July 31, 2009, 3:14 AM I would like to stain free floating sections (fixed rat brain; 50um). Is it necessary to transfer sections to "clean" culture wells with a paint brush every time I wash or incubate with antibodies? I find this causes significant damage to my tissue (especially since I do a lot of washes etc.). Could I simply use a pipette to draw out the old solution and then replace with the appropriate solution? What do most people do in these situations? Much thanks Mike _________________________________________________________________ Stay in the loop and chat with friends, right from your inbox! http://go.microsoft.com/?linkid=9671354_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Fri Jul 31 08:29:42 2009 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Fri Jul 31 08:29:47 2009 Subject: [Histonet] RE: IHC free floating sections In-Reply-To: Message-ID: <2108AECB05DBFF48A9C436A792155740011B2E19@UWHC-MAIL03.uwhis.hosp.wisc.edu> Michael: I use 1.5mm nichrome transfer loops for IHC staining of 50um free floating sections and I move them into clean culture wells for each solution. I purchase mine from Ted Pella. It does takes some practice but the loops work quite well with minimal damage to the tissue. Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Patrick Sent: Friday, July 31, 2009 2:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC free floating sections I would like to stain free floating sections (fixed rat brain; 50um). Is it necessary to transfer sections to "clean" culture wells with a paint brush every time I wash or incubate with antibodies? I find this causes significant damage to my tissue (especially since I do a lot of washes etc.). Could I simply use a pipette to draw out the old solution and then replace with the appropriate solution? What do most people do in these situations? Much thanks Mike _________________________________________________________________ Stay in the loop and chat with friends, right from your inbox! http://go.microsoft.com/?linkid=9671354_________________________________ ______________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emily <@t> pathlabsolutions.com Fri Jul 31 08:57:58 2009 From: emily <@t> pathlabsolutions.com (Emily Butte) Date: Fri Jul 31 08:58:02 2009 Subject: [Histonet] Tissue Washing Message-ID: Our lab has been struggling for a while now, with tissue washing off the slides. The problem was originally traced to our processor that was malfunctioning and having major carry-over issues, causing poor tissue processing. So after failed attempts to get the old machine fixed, we ended up getting a new microwave processor. The tissue (mainly prostate bx) comes out of the processor looking good. It cuts well, but after staining, some of the sections are no longer on the slides. The problem was quiet severe with the old processor and has decreased drastically, but has not entirely gone away with the microwave processor. Here's some info on our current procedure. This is a small physician office lab and we everything manual. We use plus slides and a water bath with no tissue adhesive added. The bath is between 42-45 degrees Celsius. After sectioning, the slide are put in an oven at 60 degrees for 20 minutes. After taking the slides out of the oven, we cool them to room temp then hand stain them in an H&E line. Xylene - 5 min Xylene - 2 min Xylene - 2 min 100% Alcohol - 30 sec 100% Alcohol - 30 sec 100% Alcohol - 30 sec 95% Alcohol - 30 sec Rinse in DiH2O Hematoxylin - 2 min Rinse in DiH2O 0.50% Acid Alcohol - 2 dips Rinse in DiH2O Scott's Tap Water - 10 sec Rinse in DiH2O 95% Alcohol - 30 sec Eosin - 30 sec 95% Alcohol - 30 sec 100% Alcohol - 30 secs 100% Alcohol - 30 secs 100% Alcohol - 30 secs Xylene - 30 sec Xylene 2 min Xylene - 2 min The slides are then cover slipped and of a 12 piece prostate case, at least a section or 2 will wash off of about 4 of the slides. Please pass along any ideas as to what the cause of this could be and how I can fix it. I'm fresh out of ideas and would really appreciate the advice of someone with more experience than myself. Thanks, -- Emily Path Lab Solutions Inc. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jul 31 09:10:20 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 31 09:10:25 2009 Subject: [Histonet] Tissue Washing Message-ID: <86ADE4EB583CE64799A9924684A0FBBF074672E0@wahtntex2.waht.swest.nhs.uk> Have you changed your Supplier for glass slides, has your Supplier changed theirs? Have you poorly quality slides, are they dirty, are they greasy. Have you tried cleaning them in alchohol by soaking and then rubbing with a cloth? If all else fails albuminise the slides but you might find that the albumin stains up too. >From my experience it usually the slides and as it has been happening for some time you may like to change to a better quality glass slide; make sure that they are not 'old' slides, you know old stock? Water OK in the water baths? Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: 31 July 2009 14:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Washing Our lab has been struggling for a while now, with tissue washing off the slides. The problem was originally traced to our processor that was malfunctioning and having major carry-over issues, causing poor tissue processing. So after failed attempts to get the old machine fixed, we ended up getting a new microwave processor. The tissue (mainly prostate bx) comes out of the processor looking good. It cuts well, but after staining, some of the sections are no longer on the slides. The problem was quiet severe with the old processor and has decreased drastically, but has not entirely gone away with the microwave processor. Here's some info on our current procedure. This is a small physician office lab and we everything manual. We use plus slides and a water bath with no tissue adhesive added. The bath is between 42-45 degrees Celsius. After sectioning, the slide are put in an oven at 60 degrees for 20 minutes. After taking the slides out of the oven, we cool them to room temp then hand stain them in an H&E line. Xylene - 5 min Xylene - 2 min Xylene - 2 min 100% Alcohol - 30 sec 100% Alcohol - 30 sec 100% Alcohol - 30 sec 95% Alcohol - 30 sec Rinse in DiH2O Hematoxylin - 2 min Rinse in DiH2O 0.50% Acid Alcohol - 2 dips Rinse in DiH2O Scott's Tap Water - 10 sec Rinse in DiH2O 95% Alcohol - 30 sec Eosin - 30 sec 95% Alcohol - 30 sec 100% Alcohol - 30 secs 100% Alcohol - 30 secs 100% Alcohol - 30 secs Xylene - 30 sec Xylene 2 min Xylene - 2 min The slides are then cover slipped and of a 12 piece prostate case, at least a section or 2 will wash off of about 4 of the slides. Please pass along any ideas as to what the cause of this could be and how I can fix it. I'm fresh out of ideas and would really appreciate the advice of someone with more experience than myself. Thanks, -- Emily Path Lab Solutions Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 31 09:22:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 31 09:22:51 2009 Subject: [Histonet] Tissue Washing In-Reply-To: Message-ID: <826998.99005.qm@web65706.mail.ac4.yahoo.com> Emily: I don't see any problems with your staining protocol. I think that the problem still resides in the processing and that you are probably having a too long dehydrating period. Also, depending on your clearing agent, that dehydrating problem can be exacerbated.Try to reduce your dehydrating steps (for small biopsies) and check the characteristics of your clearing agent. Ren? J. --- On Fri, 7/31/09, Emily Butte wrote: From: Emily Butte Subject: [Histonet] Tissue Washing To: histonet@lists.utsouthwestern.edu Date: Friday, July 31, 2009, 9:57 AM Our lab has been struggling for a while now, with tissue washing off the slides. The problem was originally traced to our processor that was malfunctioning and having major carry-over issues, causing poor tissue processing. So after failed attempts to get the old machine fixed, we ended up getting a new microwave processor. The tissue (mainly prostate bx) comes out of the processor looking good. It cuts well, but after staining, some of the sections are no longer on the slides. The problem was quiet severe with the old processor and has decreased drastically, but has not entirely gone away with the microwave processor. Here's some info on our current procedure. This is a small physician office lab and we everything manual. We use plus slides and a water bath with no tissue adhesive added. The bath is between 42-45 degrees Celsius. After sectioning, the slide are put in an oven at 60 degrees for 20 minutes. After taking the slides out of the oven, we cool them to room temp then hand stain them in an H&E line. Xylene - 5 min Xylene - 2 min Xylene - 2 min 100% Alcohol - 30 sec 100% Alcohol - 30 sec 100% Alcohol - 30 sec 95% Alcohol - 30 sec Rinse in DiH2O Hematoxylin - 2 min Rinse in DiH2O 0.50% Acid Alcohol - 2 dips Rinse in DiH2O Scott's Tap Water - 10 sec Rinse in DiH2O 95% Alcohol - 30 sec Eosin - 30 sec 95% Alcohol - 30 sec 100% Alcohol - 30 secs 100% Alcohol - 30 secs 100% Alcohol - 30 secs Xylene - 30 sec Xylene 2 min Xylene - 2 min The slides are then cover slipped and of a 12 piece prostate case, at least a section or 2 will wash off of about 4 of the slides. Please pass along any ideas as to what the cause of this could be and how I can fix it. I'm fresh out of ideas and would really appreciate the advice of someone with more experience than myself. Thanks, -- Emily Path Lab Solutions Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Fri Jul 31 09:38:15 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Jul 31 09:24:03 2009 Subject: [Histonet] Tissue Washing In-Reply-To: References: Message-ID: <4A7301D7.5070108@rci.rutgers.edu> Emily, The last time I had this problem, we switched to Ultrastick slides (ThermoFisher Scientific) and distilled water in the water bath. I haven't had any problems since with H&E staining. Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Emily Butte wrote: >Our lab has been struggling for a while now, with tissue washing off the >slides. The problem was originally traced to our processor that was >malfunctioning and having major carry-over issues, causing poor tissue >processing. So after failed attempts to get the old machine fixed, we ended >up getting a new microwave processor. The tissue (mainly prostate bx) comes >out of the processor looking good. It cuts well, but after staining, some of >the sections are no longer on the slides. The problem was quiet severe with >the old processor and has decreased drastically, but has not entirely gone >away with the microwave processor. > >Here's some info on our current procedure. This is a small physician office >lab and we everything manual. We use plus slides and a water bath with no >tissue adhesive added. The bath is between 42-45 degrees Celsius. After >sectioning, the slide are put in an oven at 60 degrees for 20 minutes. After >taking the slides out of the oven, we cool them to room temp then hand stain >them in an H&E line. >Xylene - 5 min >Xylene - 2 min >Xylene - 2 min >100% Alcohol - 30 sec >100% Alcohol - 30 sec >100% Alcohol - 30 sec >95% Alcohol - 30 sec >Rinse in DiH2O >Hematoxylin - 2 min >Rinse in DiH2O >0.50% Acid Alcohol - 2 dips >Rinse in DiH2O >Scott's Tap Water - 10 sec >Rinse in DiH2O >95% Alcohol - 30 sec >Eosin - 30 sec >95% Alcohol - 30 sec >100% Alcohol - 30 secs >100% Alcohol - 30 secs >100% Alcohol - 30 secs >Xylene - 30 sec >Xylene 2 min >Xylene - 2 min > >The slides are then cover slipped and of a 12 piece prostate case, at least >a section or 2 will wash off of about 4 of the slides. Please pass along any >ideas as to what the cause of this could be and how I can fix it. I'm fresh >out of ideas and would really appreciate the advice of someone with more >experience than myself. > >Thanks, > > From pattennj <@t> mail.nih.gov Fri Jul 31 09:03:15 2009 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Fri Jul 31 09:25:51 2009 Subject: [Histonet] Heterochromatin Ab Message-ID: Hello- I'm wondering if anyone can recommend a good antibody that specifically labels heterochromatin (IF on FFPE sections). I've looked into several histone markers and HP1 (although I'm unsure which subtype would be best), but I'd prefer to use an antibody that I know has worked for someone else! Any help would be appreciated. Thanks! -Nicole NIAAA/National Institutes of Health From sprice2003 <@t> gmail.com Fri Jul 31 10:30:47 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Fri Jul 31 10:30:52 2009 Subject: [Histonet] RE:Antigen retrieval paraffin skin samples Message-ID: Maybe I've misinterpreted Nick's posting, but I think what he's looking for is what kinds of reagents are used for retrieving Ck antigens. It may something to do with the PFA fixation, but I'm not too sure about using ficin for CKs, We usually use other enzymes or heat. Proteinase K, or trypsin, or citrate solutions work really well in most cases. Sally ------------------------------ Message: 8 Date: Thu, 30 Jul 2009 09:30:37 -0600 From: "Sorenson, Jon (Nampa)" Subject: [Histonet] RE:Antigen retrieval paraffin skin samples To: histonet@lists.utsouthwestern.edu We use and like the PASCAL pressure cooker system from DAKO, it is uniform, convenient and fairly Easy to use. Jon Sorenson Histology Coordinator Mercy Medical Center jonsorenso@chiwest.com 208-463-5267 ------------------------------ Message: 17 Date: Wed, 29 Jul 2009 14:05:29 -0700 (PDT) From: "Nicholas David Evans" Subject: [HISTONET] Antigen retrieval paraffin skin samples To: Dear all, Might anyone be able to offer their advice on the best method to retrieve antigens on paraffin embedded skin sections? I am immunostaining for epithelial cytokeratins, and I have block-to-block variation in the quality of the staining - some block give consistently good staining while others giving negligible staining. The tissues were prepared by fixation in 4% PFA for 24 - 48 hours followed by dehydration and embedding. I currently use 15 mins Ficin digestion. I suspect that the differences may be accounted for by variability in the length of time samples were fixed in PFA and then 70% ethanol. Best wishes Nick From Montina.VanMeter <@t> pbrc.edu Fri Jul 31 11:05:36 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Jul 31 11:05:43 2009 Subject: [Histonet] RE: IHC free floating sections In-Reply-To: <2108AECB05DBFF48A9C436A792155740011B2E19@UWHC-MAIL03.uwhis.hosp.wisc.edu> References: <2108AECB05DBFF48A9C436A792155740011B2E19@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: <4FE7FB862E90E448AE32388E759220E501822FDA@pbrcas31.pbrc.edu> Mike, I free float all of my rat brain sections (40-60um) and transfer them with a paintbrush. It takes a delicate touch to do this without causing damage to the tissue. I do know people who use pipettes to change the solutions. Either way works, it's a matter of personal preference. There are plastic vessels with mesh screens that can be purchased commercially, that allow you to transfer tissue without touching them. The only problem with that method is it requires additional amounts of antibody. For example: I dispense 500ul of antibody+diluent into the well when using the paintbrush transfer method (this is done under constant agitation on a shaker table). The plastic tissue vessel method requires 2 ml. of antibody+diluent (to cover tissue with enough fluid to account for the size of the vessel within the well itself). The link below offers commercially made tissue transfer vessels (look under circular staining nets and dishes). http://www.brainresearchlab.com/productinform.html Good luck, Tina Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Friday, July 31, 2009 8:30 AM To: Michael Patrick; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC free floating sections Michael: I use 1.5mm nichrome transfer loops for IHC staining of 50um free floating sections and I move them into clean culture wells for each solution. I purchase mine from Ted Pella. It does takes some practice but the loops work quite well with minimal damage to the tissue. Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Patrick Sent: Friday, July 31, 2009 2:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC free floating sections I would like to stain free floating sections (fixed rat brain; 50um). Is it necessary to transfer sections to "clean" culture wells with a paint brush every time I wash or incubate with antibodies? I find this causes significant damage to my tissue (especially since I do a lot of washes etc.). Could I simply use a pipette to draw out the old solution and then replace with the appropriate solution? What do most people do in these situations? Much thanks Mike _________________________________________________________________ Stay in the loop and chat with friends, right from your inbox! http://go.microsoft.com/?linkid=9671354_________________________________ ______________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Cordero <@t> comphealth.com Fri Jul 31 11:44:56 2009 From: Robert.Cordero <@t> comphealth.com (Robert.Cordero@comphealth.com) Date: Fri Jul 31 11:45:19 2009 Subject: [Histonet] - New Histo/IHC Opportunity in Southern NC! Message-ID: Hello! I wanted to put the word out of a new Histology opening in North Carolina for a strong and experienced HT/HTL with extensive IHC experience. The facility is offering great benefits, competitive salary and an ASAP start date. This is a Mon-Fri day shift for a private lab. They are also offering relocation assistance for any applicants who would be looking to move to the area. ASCP certification is a must. If you are interested or know of someone that is, please let me know immediately. Regards, Rob Cordero Laboratory Sciences Consultant Permanent Placement Division CompHealth Associates, Inc. Phone: 866.782.9029 x. 5866 Direct: 954.343.5866 Fax: 800.420.2329 E-mail: robert.cordero@comphealth.com www.comphealth.com About us: http://www.comphealth.com/about_us/about Please note: "This is a commercial email from CompHealth Associates, Inc. If you do not want to receive future emails from CompHealth Associates, Inc., please reply to the sender of this email and ask to be removed from our list." From carl.hobbs <@t> kcl.ac.uk Fri Jul 31 12:01:59 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Jul 31 12:05:59 2009 Subject: [Histonet] Re: IHC free floating sections Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742977@KCL-MAIL04.kclad.ds.kcl.ac.uk> Apologies if I have not adhered to Histonet protocol: I have changed my Browser from IE8 to latest Firefox and am not yet familiar! I agree with Rene: stay with the same wells and give additional, longer washes. Imho, even experienced users suffer section damage if they change wells. From carl.hobbs <@t> kcl.ac.uk Fri Jul 31 12:15:32 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Jul 31 12:15:51 2009 Subject: [Histonet] Re: Tissue Washing Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742978@KCL-MAIL04.kclad.ds.kcl.ac.uk> Again, apologies if I have messed up with message delivery : Firefox from IE is a learning curve after IE for many years. Re your problem: only part that I reckon that is your problem is ONLY using ~20mins in an oven. Leave for 2hrs at 60C, then stain.( overnight if you can is great for H&Es) See if this makes any difference. Then take them out of the oven and straight into Xylene....no need to cool: if they are going to stay on, cooling will play NO part in additional adhesion. Occam. From Todd.Krueger <@t> bsci.com Fri Jul 31 12:37:01 2009 From: Todd.Krueger <@t> bsci.com (Krueger, Todd) Date: Fri Jul 31 12:37:16 2009 Subject: [Histonet] zinc formalin Message-ID: <669973B45D49DA43B0FA9AC9609CBF540264F865@MAPMAIL01.bsci.bossci.com> Can anyone who uses zinc formalin as a fixative tell me if there are any special stains that seem to be adversely affected by it. Also do you use it for long term storage or do you exchange it with 10% NBF. Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krueger@bsci.com From deliadfam <@t> yahoo.com Fri Jul 31 13:41:30 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Fri Jul 31 13:41:33 2009 Subject: [Histonet] Please Help! Message-ID: <485652.58669.qm@web63106.mail.re1.yahoo.com> Hello, I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much. Delia From barrickstacey <@t> yahoo.com Fri Jul 31 14:28:11 2009 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Fri Jul 31 14:28:14 2009 Subject: [Histonet] Please Help! In-Reply-To: <485652.58669.qm@web63106.mail.re1.yahoo.com> Message-ID: <291747.5041.qm@web54304.mail.re2.yahoo.com> You might have some luck trying this - Wipe out the cryostat with a dryer sheet to remove the static. This trick works for me alot. Sometimes you need to repeat it a few times during cutting. Wiping the cold plate withthe dryer sheet works wonders for me! ? Good Luck!! Stacey --- On Fri, 7/31/09, DELIA GARCIA wrote: From: DELIA GARCIA Subject: [Histonet] Please Help! To: histonet@lists.utsouthwestern.edu Date: Friday, July 31, 2009, 2:41 PM Hello, I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much. Delia ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ndevans <@t> stanford.edu Fri Jul 31 14:36:43 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Fri Jul 31 14:36:47 2009 Subject: [Histonet] Please Help! In-Reply-To: <485652.58669.qm@web63106.mail.re1.yahoo.com> References: <485652.58669.qm@web63106.mail.re1.yahoo.com> Message-ID: <001d01ca1216$39d7b9a0$ce1d41ab@DellDesktop2> We fix skin by sandwiching it between two sponges soaked in either 4% PFA or 0.4%PFA (for paraffin or cryo respectively) and compressed in a cassette. This flattens the skin. Following several hours fixation the skin is usually flat and rigid enough to handle and embed. It can then either by dehydrated for paraffin or soaked in sucrose for cryo. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA Sent: Friday, July 31, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please Help! Hello, I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much. Delia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Jul 31 14:54:42 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jul 31 14:54:54 2009 Subject: [Histonet] Please Help! References: <485652.58669.qm@web63106.mail.re1.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2AD7@fhosxchmb006.ADVENTISTCORP.NET> I do several skin frozens every week and I know exactly how frustrating it can be when they roll! However, the technique I use is simple : As the edge of the skin section that is rolling is about to make contact with the knife/blade, put your soft brush bristles on top of the section and keep them on the section as you cut and the section rolls off the chuck. If you're lucky the section will stay down on the plate and you can gently use the brush to tease the edges flat. Then, you need to be fast and get it on the slide. Once in a while I'll get a case that needs a fresh area of the blade after trimming to give me a decent section with the technique. I hope this works for you. Have a great weekend! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of DELIA GARCIA Sent: Fri 7/31/2009 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please Help! Hello, I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much. Delia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From laurie <@t> conxis.com Fri Jul 31 15:57:52 2009 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Fri Jul 31 15:58:03 2009 Subject: [Histonet] tissue submission question Message-ID: <7DBD932BC2F34598871215B1FCC111D4.MAI@accuwebhosting.biz> Happy Friday everyone. I have an investigator that wants to submit leg muscle from a rat for IHC staining. The problem is they did not dissect the muscle out after euthanizing the animal, they just froze the whole animal. We do not have a sliding microtome so that we can just "slice" the animal up ( yes the investigator called it slicing.) Any thoughts out there? No this is not a Fun Friday joke :-( Have a great weekend! Laurie Laurie Popp, BA HT ( ASCP) **************************************** From deliadfam <@t> yahoo.com Fri Jul 31 23:45:41 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Fri Jul 31 23:45:45 2009 Subject: [Histonet] from PLEASE HELP Message-ID: <711193.76471.qm@web63107.mail.re1.yahoo.com> Thank you all for your imput. I tried thinning out the sections to 3 mircrons and for some reason cut a bit better. There's a few more things you all mentioned Im going to try. The thing with the punch biopsies is that when when i'm cutting the frozen the actual tissue is what curls leaving a hole in the OTC with a tightly curled piece of tissue in the middle. Very hard to try to brush it out. I have no problem cutting shaves or excisions but the punches are something else. A little more info.... They come in Michel's fixative, I embed in OCT,? freeze, cut and place in acetone for 10 min.. Skin punches is usually what I get on a FITC request.?Again thanks I'm glad I?shared this issue with you. Delia