[Histonet] Autoflourescence

[Bob Nienhuis]

The Wright Cell Imaging Facility at University Health Network
in Toronto has compiled information on reducing autofluorescence
from the Histonet archives into a booklet.
Very useful.

http://www.uhnres.utoronto.ca/facilities/wcif/fdownload2.html

Bob


On Thu, Jan 15, 2009 at 1:36 PM, Patten, Nicole (NIH/NIAAA) [F] <
pattennj <@t> mail.nih.gov> wrote:

> Hi-
>
> I am having a horrible time with autofluorescence in my human brain FFPE
> tissue. I have been using Sudan Black which helps a little, but the
> background is still pretty bad. The tissue is usually cut at 10-15um.
>
> Does anyone have any other suggestions? Has anyone tried photobleaching
> the tissue prior to staining with any success? I attempted this once
> with no luck...
>
> Any help would be very much appreciated! Thanks in advance.
>
> Nicole
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>