From tifei <@t> foxmail.com Fri Jan 2 07:15:00 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Jan 2 07:16:07 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody References: , , <200812301644573521912@foxmail.com>, , <200812310325559009046@foxmail.com>, Message-ID: <200901022114549441081@foxmail.com> Hi Just wondering whether the antibody for WB (only WB, IP on datasheet) can be used for IHC use? If not, why? Can I just increase the concentration for IHC application? I want to learn more about the underlying production processes. Thanks! 2009-01-02 TF From drvet_anjan <@t> hotmail.com Fri Jan 2 10:29:38 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Fri Jan 2 10:29:43 2009 Subject: [Histonet] IgM monoclonal antibody compatibility Message-ID: hi, i wanted to know if there are any difference in selecting secondary antibody against IgM compared to secondary antibody for IgG. as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary antibody should i select, currently i m hoping that goat anti mouse will work. can any one plz suggest me on this. Regards, Dr. Anjan Kumar Junior Research Scientist _________________________________________________________________ Much more than email ? don't miss out on the rest of the Windows Live?. http://www.microsoft.com/windows/windowslive/ From NSEARCY <@t> swmail.sw.org Fri Jan 2 10:38:49 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Jan 2 10:38:54 2009 Subject: [Histonet] 2009 RVU Values Message-ID: <495DEEB9020000EF0001323E@MERCURY.SW.ORG> Anyone out there received the new values for 2009 that start today? Thanks Nita From jqb7 <@t> cdc.gov Fri Jan 2 12:17:38 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jan 2 12:17:56 2009 Subject: [Histonet] RE: [IHCRG] Ventana vs Dako Special stainers In-Reply-To: <806574CA2B466B4CB1DF2DFC5F864B725C3C421755@KHSMAIL.kennedyhealth.org> References: <806574CA2B466B4CB1DF2DFC5F864B725C3C421755@KHSMAIL.kennedyhealth.org> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402ACEE@LTA3VS011.ees.hhs.gov> Have not tried Ventana but are very happy with the Dako Artisan. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Wendt, Karl Sent: Friday, January 02, 2009 1:16 PM To: ihcrg@googlegroups.com Subject: [IHCRG] Ventana vs Dako Special stainers Happy New Year to All! I have a non-IHC question. We are looking at automating our special stains. So far we have looked at Ventana's Nexes and I'm not all that happy, especially with the barcode problems and fluid heating system. I would like people's input (good or bad) about Ventana's Nexes versus Dako's Artisan staining system. Thanks, Karl Wendt Pathology Coordinator Kennedy Health System ________________________________ -------------------------------------- This message contains information that may be confidential and privileged. Unless you are the addressee (or authorized to receive on behalf of the addressee), you may not use, copy, forward, or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and to internethelp@kennedyhealth.org, and delete the message. Thank you. --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From JWeems <@t> sjha.org Fri Jan 2 12:25:05 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jan 2 12:25:15 2009 Subject: [Histonet] RE: [IHCRG] Ventana vs Dako Special stainers In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70402ACEE@LTA3VS011.ees.hhs.gov> References: <806574CA2B466B4CB1DF2DFC5F864B725C3C421755@KHSMAIL.kennedyhealth.org> <1CE1847DFEA0A647B1CCDE4108EA60A70402ACEE@LTA3VS011.ees.hhs.gov> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA52090EB@ITSSSXM01V6.one.ads.che.org> We do as well. Happy 2009 Everybody!!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, January 02, 2009 1:18 PM To: k.wendt@kennedyhealth.org; histonet Subject: [Histonet] RE: [IHCRG] Ventana vs Dako Special stainers Have not tried Ventana but are very happy with the Dako Artisan. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Wendt, Karl Sent: Friday, January 02, 2009 1:16 PM To: ihcrg@googlegroups.com Subject: [IHCRG] Ventana vs Dako Special stainers Happy New Year to All! I have a non-IHC question. We are looking at automating our special stains. So far we have looked at Ventana's Nexes and I'm not all that happy, especially with the barcode problems and fluid heating system. I would like people's input (good or bad) about Ventana's Nexes versus Dako's Artisan staining system. Thanks, Karl Wendt Pathology Coordinator Kennedy Health System ________________________________ -------------------------------------- This message contains information that may be confidential and privileged. Unless you are the addressee (or authorized to receive on behalf of the addressee), you may not use, copy, forward, or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and to internethelp@kennedyhealth.org, and delete the message. Thank you. --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From talulahgosh <@t> gmail.com Fri Jan 2 13:25:43 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 2 13:26:41 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody In-Reply-To: <200901022114549441081@foxmail.com> References: <200812301644573521912@foxmail.com> <200812310325559009046@foxmail.com> <200901022114549441081@foxmail.com> Message-ID: If anyone replies with posting to the list, please forward it. I've always wondered the same thing. On Fri, Jan 2, 2009 at 8:15 AM, TF wrote: > Hi > Just wondering whether the antibody for WB (only WB, IP on datasheet) can > be used for IHC use? > If not, why? > > Can I just increase the concentration for IHC application? > > I want to learn more about the underlying production processes. > Thanks! > > > 2009-01-02 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- At the end of what is called the "sexual life" the only love which has lasted is the love which has everything, every disappointment, every failure and every betrayal, which has accepted even the sad fact that in the end there is no desire so deep as the simple desire for companionship. -Graham Greene From kgreicar <@t> thepathlab.com Fri Jan 2 13:27:02 2009 From: kgreicar <@t> thepathlab.com (Kipp Greicar) Date: Fri Jan 2 13:27:39 2009 Subject: [Histonet] IHC automated stainers Message-ID: Is anyone using either the biogenics xmatrix or the biocare intellipath? I am shopping and could sure use some feedback. Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 62, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Differences between WB antibody and IHC antibody (TF) 2. IgM monoclonal antibody compatibility (anjan kumar) 3. 2009 RVU Values (Nita Searcy) ---------------------------------------------------------------------- Message: 1 Date: Fri, 2 Jan 2009 21:15:00 +0800 From: "TF" Subject: [Histonet] Differences between WB antibody and IHC antibody To: "Anatoli Gleiberman" Cc: histonet Message-ID: <200901022114549441081@foxmail.com> Content-Type: text/plain; charset="gb2312" Hi Just wondering whether the antibody for WB (only WB, IP on datasheet) can be used for IHC use? If not, why? Can I just increase the concentration for IHC application? I want to learn more about the underlying production processes. Thanks! 2009-01-02 TF ------------------------------ Message: 2 Date: Fri, 2 Jan 2009 21:59:38 +0530 From: anjan kumar Subject: [Histonet] IgM monoclonal antibody compatibility To: triple immunohistochem Message-ID: Content-Type: text/plain; charset="Windows-1252" hi, i wanted to know if there are any difference in selecting secondary antibody against IgM compared to secondary antibody for IgG. as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary antibody should i select, currently i m hoping that goat anti mouse will work. can any one plz suggest me on this. Regards, Dr. Anjan Kumar Junior Research Scientist _________________________________________________________________ Much more than email  don't miss out on the rest of the Windows Live. http://www.microsoft.com/windows/windowslive/ ------------------------------ Message: 3 Date: Fri, 02 Jan 2009 10:38:49 -0600 From: "Nita Searcy" Subject: [Histonet] 2009 RVU Values To: Message-ID: <495DEEB9020000EF0001323E@MERCURY.SW.ORG> Content-Type: text/plain; charset=US-ASCII Anyone out there received the new values for 2009 that start today? Thanks Nita ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 2 *************************************** From koellingr <@t> comcast.net Fri Jan 2 13:56:30 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Jan 2 13:56:36 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody Message-ID: <010220091956.23646.495E716E0000A00E00005C5E22007637049D09020704040A0105@comcast.net> TF and Emily, You can use antibodies designed for Western blots. They can work. In a western blot, a lot of times these are run under denaturing conditions (a type of soap is used to break the secondary and tertiary structure) and so the epitope seen is linear. That antibody for Western's works at a linear epitope (which is only a few small amino acids out of the whole proten structure). Your tissue IHC is based on proteins that have not been linearized and are more native. So if it happens that the antibody recognizes an epitope that happens to be linear within the non-linear protein, it certainly will work. If it happens to be an epitope that is hidden away within the 3-D native structure and has not been linearized, it won't work. There are WB that use (native) non-denaturing protocols so if the antibody works in that situation it might very well work in IHC. There are protocols for placing tissue sections in SDS to try to emulate the conditions of a denaturing WB to be able to utiliz e those antibodies, but these are not very standardized or widely accepted procedures. Have used successfully WB antibodies on tissue IHC with almost no trouble. Other WB antibodies I couldn't get to work on IHC despite heroic efforts. Is all empirical; you just have to try. Raymond Koelling Research Scientist PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Emily Sours" > If anyone replies with posting to the list, please forward it. I've always > wondered the same thing. > > On Fri, Jan 2, 2009 at 8:15 AM, TF wrote: > > > Hi > > Just wondering whether the antibody for WB (only WB, IP on datasheet) can > > be used for IHC use? > > If not, why? > > > > Can I just increase the concentration for IHC application? > > > > I want to learn more about the underlying production processes. > > Thanks! > > > > > > 2009-01-02 > > > > > > > > TF > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > At the end of what is called the "sexual life" the only love which has > lasted is the love which has everything, every disappointment, every failure > and every betrayal, which has accepted even the sad fact that in the end > there is no desire so deep as the simple desire for companionship. > -Graham Greene > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Fri Jan 2 14:03:50 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Jan 2 14:05:27 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody In-Reply-To: References: <200812301644573521912@foxmail.com><200812310325559009046@foxmail.com><200901022114549441081@foxmail.com> Message-ID: You can possibly use it especially if it has been used for IF. What you need to do is to get a slide that has a known positive for the antibody, try the dilution for WB with your standard protocol and see if it works. If is too light or too dark you will have to titer it (which should be done in the first place). If there is no staining you may have to try different things like enzymatic antigen retrieval or heat etc. In most cases you can get it to work to some degree but it is better if you get one that has been used with tissue. The first thing I do is call the company and talk to the technical personnel to see if they have tested it on tissue samples. It takes some work. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, January 02, 2009 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Differences between WB antibody and IHC antibody If anyone replies with posting to the list, please forward it. I've always wondered the same thing. On Fri, Jan 2, 2009 at 8:15 AM, TF wrote: > Hi > Just wondering whether the antibody for WB (only WB, IP on datasheet) can > be used for IHC use? > If not, why? > > Can I just increase the concentration for IHC application? > > I want to learn more about the underlying production processes. > Thanks! > > > 2009-01-02 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- At the end of what is called the "sexual life" the only love which has lasted is the love which has everything, every disappointment, every failure and every betrayal, which has accepted even the sad fact that in the end there is no desire so deep as the simple desire for companionship. -Graham Greene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From anh2006 <@t> med.cornell.edu Fri Jan 2 16:14:12 2009 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Fri Jan 2 16:14:14 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody In-Reply-To: References: <200812301644573521912@foxmail.com> <200812310325559009046@foxmail.com> <200901022114549441081@foxmail.com> Message-ID: Whether or not an antibody can be used for western vs IHC depends on many factors. Some antibodies can be used for both, some can be only used for one vs another, and some cannot be used for either. There are quite a few factors to consider and here are a few from the top of my head (and I am hoping people can add to this list): (1) Epitope recognition Western blotting often times (but not always of course) involves the denaturation of proteins as well as breaking of disulfide bonds etc. Samples are often boiled in denaturing agents such as beta-mercaptoethanol or DTT and are run in SDS containing buffers and gels. In doing this certain epitopes may be revealed enabling a specific antibody to work on western vs IHC. Along those same lines, this denaturation of proteins can also destroy epitopes, because proteins are no longer in their native conformations, disabling the efficacy of certain antibodies. Similarly, IHC involves fixation, processing, sectioning, heat retrieval etc. Fixation alone can alter epitopes dramatically thereby precventing a certain antibody from binding. On the contrary, proteins are often well preserved in a native conformation in IHC (especially in fresh frozen sections). (2) Access Because western blotting involves protein lysates of cells which have been busted open by a variety of methods, all of the proteins in a cell should be accessible to the antibody. In IHC on sections or on coverslips etc, the cell structures are largely preserved intact. Therefore certain cell compartments may need further processing to grant access of the big bulky antibody to the inside of the compartments. Hence part of the reason why we do "antigen retrieval" with enzymes and detergents etc. Similarly because in western, one might spin out fractions before running on a gel if you are searching for a nuclear protein or other protein localized to a certain compartment you must be sure you actually have the compartment preserved in a western prep where you always would have it present in an intact IHC section. (3) Sensitivity Western blotting is superior to run-of-the-mill IHC in detection sensitivity. This is even more obvious when one considers how easy it is to interpret weak or sparse IHC staining as background. Background is much easier to interpret in a western blot. Therefore an antibody which works beautifully in WB may work in IHC but the proteins might be too sparse or too few in number to be visualized. (4) Polyclonal vs. monoclonal If an antibody is a polyclonal antibody (pAb) it will likely have a better chance to work across a broad spectrum of applications. This is because a polyclonal antibody preparation is actually a combination of different Ig's recognizing more than one epitope (although it is important to know that there may be a predominant Ig present or the pAb could have been purified in a way to enrich for a particular antigen which may alter it's ability to work in a broad range of apps). That's all I can think of for now, and I hope this makes sense ... but as someone else said, it's all empirical and has to be tested in each person's lab. Happy New Year! >If anyone replies with posting to the list, please forward it. I've always >wondered the same thing. > >On Fri, Jan 2, 2009 at 8:15 AM, TF wrote: > > > Hi > > Just wondering whether the antibody for WB (only WB, IP on datasheet) can >> be used for IHC use? >> If not, why? >> >> Can I just increase the concentration for IHC application? >> >> I want to learn more about the underlying production processes. >> Thanks! >> >> >> 2009-01-02 >> >> >> > > TF -- From tifei <@t> foxmail.com Fri Jan 2 21:16:30 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Fri Jan 2 21:16:41 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gSWdNIG1vbm9jbG9uYWwgYW50aWJvZHkgY29tcGF0aWJpbGl0eQ==?= References: Message-ID: <200901031116246115643@foxmail.com> I have the same post last week but did not get reply. Actually I have a IgM primary antibody and I can just visualize the staining with IgG 2nd antibody. SOme IgG 2nd antibody can just applied to do so. This has been proved. But you should test yours anyway. 2009-01-03 TF ???? anjan kumar ????? 2009-01-03 00:33:39 ???? triple immunohistochem ??? ??? [Histonet] IgM monoclonal antibody compatibility hi, i wanted to know if there are any difference in selecting secondary antibody against IgM compared to secondary antibody for IgG. as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary antibody should i select, currently i m hoping that goat anti mouse will work. can any one plz suggest me on this. Regards, Dr. Anjan Kumar Junior Research Scientist _________________________________________________________________ Much more than email ? don't miss out on the rest of the Windows Live?. http://www.microsoft.com/windows/windowslive/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu From tifei <@t> foxmail.com Fri Jan 2 21:27:59 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Jan 2 21:28:14 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody References: , , <200812301644573521912@foxmail.com>, , <200812310325559009046@foxmail.com>, , <200901022114549441081@foxmail.com>, Message-ID: <200901031127539085599@foxmail.com> VGhhbmtzIHZlcnkgbXVjaCENCg0KaSBhbSByZWZlcmluZyB0bzogDQoNCkRvdWJsZWNvcnRpbiAo SC0yODApIEFudGlib2R5IHNjLTI4OTM5DQpodHRwOi8vd3d3LnNjYnQuY29tL2RhdGFzaGVldC0y ODkzOS1kb3VibGVjb3J0aW4taC0yODAtYW50aWJvZHkuaHRtbCANCkRvdWJsZWNvcnRpbiAoSC0y 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Yi4NCg0KDQpIYXBweSBOZXcgWWVhciENCg0KDQoNCg0KSWYgYW55b25lIHJlcGxpZXMgd2l0aCBw b3N0aW5nIHRvIHRoZSBsaXN0LCBwbGVhc2UgZm9yd2FyZCBpdC4gIEkndmUgYWx3YXlzDQp3b25k ZXJlZCB0aGUgc2FtZSB0aGluZy4NCg0KDQpPbiBGcmksIEphbiAyLCAyMDA5IGF0IDg6MTUgQU0s IFRGIDx0aWZlaUBmb3htYWlsLmNvbT4gd3JvdGU6DQoNCg0KPiBIaQ0KPiBKdXN0IHdvbmRlcmlu ZyB3aGV0aGVyIHRoZSBhbnRpYm9keSBmb3IgV0IgKG9ubHkgV0IsIElQIG9uIGRhdGFzaGVldCkg Y2FuDQo+IGJlIHVzZWQgZm9yIElIQyB1c2U/DQo+IElmIG5vdCwgd2h5Pw0KPg0KPiBDYW4gSSBq dXN0IGluY3JlYXNlIHRoZSBjb25jZW50cmF0aW9uIGZvciBJSEMgYXBwbGljYXRpb24/DQo+DQo+ IEkgd2FudCB0byBsZWFybiBtb3JlIGFib3V0IHRoZSB1bmRlcmx5aW5nIHByb2R1Y3Rpb24gcHJv Y2Vzc2VzLg0KPiBUaGFua3MhDQo+DQo+DQo+IDIwMDktMDEtMDINCj4NCj4NCj4NCj4gVEYNCg0K DQotLSANCg== From arvidsonkristen <@t> yahoo.com Sat Jan 3 06:42:19 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sat Jan 3 06:42:22 2009 Subject: [Histonet] (no subject) Message-ID: <647119.46751.qm@web65706.mail.ac4.yahoo.com> Hello all, ? I am looking for info on IP stainers.? Which stainers are people using?? What does everyone like, dislike, etc.? Any info is helpful.? Thanks!! ? Kristen From rjbuesa <@t> yahoo.com Sat Jan 3 09:44:08 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 3 09:44:12 2009 Subject: [Histonet] (no subject) In-Reply-To: <647119.46751.qm@web65706.mail.ac4.yahoo.com> Message-ID: <201997.5271.qm@web65701.mail.ac4.yahoo.com> Almost every main laboratory instruments manufacturer has one. I have used the Ventana and the DAKO autostainers and for me the DAKO is better. Ren? J. --- On Sat, 1/3/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] (no subject) To: "histonet" Date: Saturday, January 3, 2009, 7:42 AM Hello all, ? I am looking for info on IP stainers.? Which stainers are people using?? What does everyone like, dislike, etc.? Any info is helpful.? Thanks!! ? Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpastor1 <@t> nycap.rr.com Sat Jan 3 18:07:00 2009 From: jpastor1 <@t> nycap.rr.com (Joseph N. Pastore) Date: Sat Jan 3 18:07:10 2009 Subject: [Histonet] colon resection fixation Message-ID: On our last HistoQuip it was mentioned that our section for a colon specimen was under fixed or poorly fixed. Now ...we routinely fix in formalin overnite and the overnite in O-Fix. But there are times when the specimen has to be stored in the OR refrig after COB. Can this be the problem and does anyone have ideas as to how this can be avoided? From rjbuesa <@t> yahoo.com Sun Jan 4 11:08:44 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 4 11:09:45 2009 Subject: [Histonet] colon resection fixation In-Reply-To: Message-ID: <453669.4993.qm@web65715.mail.ac4.yahoo.com> Most definitely that could be the problem. A colon in the refrigerator, although it will not be rotten, the cellular detail will be affected. You have to coordinate with the OR for the colon to be brought to histology and start fixing immediately, anything else will affect the quality of the microscopic image. Ren? J. --- On Sat, 1/3/09, Joseph N. Pastore wrote: From: Joseph N. Pastore Subject: [Histonet] colon resection fixation To: histonet@lists.utsouthwestern.edu Date: Saturday, January 3, 2009, 7:07 PM On our last HistoQuip it was mentioned that our section for a colon specimen was under fixed or poorly fixed. Now ...we routinely fix in formalin overnite and the overnite in O-Fix. But there are times when the specimen has to be stored in the OR refrig after COB. Can this be the problem and does anyone have ideas as to how this can be avoided? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonetalias <@t> gmail.com Sun Jan 4 11:17:08 2009 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Sun Jan 4 11:18:06 2009 Subject: [Histonet] (no subject) In-Reply-To: <647119.46751.qm@web65706.mail.ac4.yahoo.com> References: <647119.46751.qm@web65706.mail.ac4.yahoo.com> Message-ID: <4b6c85510901040917t44560adaw2aa661cf27f9c77e@mail.gmail.com> Demo them all then decide which one works best for you. On Sat, Jan 3, 2009 at 7:42 AM, kristen arvidson wrote: > Hello all, > > I am looking for info on IP stainers. Which stainers are people using? > What does everyone like, dislike, etc. Any info is helpful. Thanks!! > > Kristen > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From pruegg <@t> ihctech.net Sun Jan 4 11:49:53 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jan 4 11:50:59 2009 Subject: [Histonet] Differences between WB antibody and IHC antibody In-Reply-To: References: <200812301644573521912@foxmail.com><200812310325559009046@foxmail.com><200901022114549441081@foxmail.com> Message-ID: <15FBAC16AD1F48BFAB7EA2B0DE83E69C@ihctechq9h2qof> As Ray states it is all empirical, some will work from WB to IHC and some will not in my experience. In general if the spec sheet recommends a dilution for WB at say 1:2000, I will start using the ab for IHC on tissue at 1:200 (it will definitely need to be tittered and used at probably around 10 fold more concentrated for IHC on tissue). I like getting info on the ab for WB and sometimes that may be all you get. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, January 02, 2009 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Differences between WB antibody and IHC antibody If anyone replies with posting to the list, please forward it. I've always wondered the same thing. On Fri, Jan 2, 2009 at 8:15 AM, TF wrote: > Hi > Just wondering whether the antibody for WB (only WB, IP on datasheet) can > be used for IHC use? > If not, why? > > Can I just increase the concentration for IHC application? > > I want to learn more about the underlying production processes. > Thanks! > > > 2009-01-02 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- At the end of what is called the "sexual life" the only love which has lasted is the love which has everything, every disappointment, every failure and every betrayal, which has accepted even the sad fact that in the end there is no desire so deep as the simple desire for companionship. -Graham Greene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jan 4 11:58:09 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jan 4 11:58:15 2009 Subject: [Histonet] IgM monoclonal antibody compatibility In-Reply-To: References: Message-ID: <18643A031DFF44C99AE79B5FB444F624@ihctechq9h2qof> There is definitely a difference in selecting the matching isotype of secondary for IHC. Many people learned that lesion back in the day trying to stain for Hodgkins with CD15 which was IgM, we would get negative results because the secondary reagents were just recognizing IgG. It is my understanding that most of the detection systems commercially sold today will include more than just IgG to accommodate the use of abs that may be IgM? You can use the fact that different abs recognize different Ig subsets as a tool to do multi labeling in the same tissue, so it can be a good thing. If you have one ab that is IgG you could detect it with anti IgG Hrp for instances, and then if you have a second ab IgM you could detect that with anti IgM AP for instance and get double labeling even if both abs are from the same animal species. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Friday, January 02, 2009 9:30 AM To: triple immunohistochem Subject: [Histonet] IgM monoclonal antibody compatibility hi, i wanted to know if there are any difference in selecting secondary antibody against IgM compared to secondary antibody for IgG. as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary antibody should i select, currently i m hoping that goat anti mouse will work. can any one plz suggest me on this. Regards, Dr. Anjan Kumar Junior Research Scientist _________________________________________________________________ Much more than email - don't miss out on the rest of the Windows LiveT. http://www.microsoft.com/windows/windowslive/_______________________________ ________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pritchm <@t> ccf.org Sun Jan 4 12:37:16 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Sun Jan 4 12:37:24 2009 Subject: [Histonet] DNA streaming out of nuclei in frozen sections? Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021512E0@CCHSCLEXMB56.cc.ad.cchs.net> Hello: I have recently begun to use frozen sections to localize an antigen found in mouse liver tissue. My previous experience has been almost exclusively with staining of formalin-fixed, paraffin-embedded tissues. I have done two runs of this new staining protocol and noticed an odd artifact in both runs. It appears as though there are stringy stars throughout the sections! ?The 'strings' seem to originate from the hepatocyte nuclei leading me to believe that what I am seeing is DNA that has escaped the confines of the nucleus and is streaming out over the tissue. ?I did not squish or drag the coverslips onto/over the sections; this is the only thing that I could imagine would make such a mess. Does anyone have any information on the nature of this artifact and how to prevent it? I will be happy to provide my protocol or a picture if this is an odd artifact with which no one has experience. I suspect, however, that this is a 'newbie' mistake so I will refrain from posting any additional information at the present time. Thanks for your help. ---M.T. Pritchard Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 ? phone:? 216.444.8613 fax:? 216.636.1493 ? email:? pritchm@ccf.org ? Lab location: Lerner Research Institute NE4-214 =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From rjbuesa <@t> yahoo.com Sun Jan 4 14:18:35 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 4 14:19:33 2009 Subject: [Histonet] DNA streaming out of nuclei in frozen sections? In-Reply-To: <7CEB62F1535B9E44AD8A5FEFB2E10F6E021512E0@CCHSCLEXMB56.cc.ad.cchs.net> Message-ID: <897444.89700.qm@web65713.mail.ac4.yahoo.com> First I would determine if it is really DNA streaming out of the nuclei. Stain the sections with the Feulgen-Rossenbeck method, then I would also review the speed of freezing the tissue before FS, usually freezing time produce many artifacts in the final sections. Ren? J. --- On Sun, 1/4/09, Pritchard, Michele wrote: From: Pritchard, Michele Subject: [Histonet] DNA streaming out of nuclei in frozen sections? To: histonet@lists.utsouthwestern.edu Date: Sunday, January 4, 2009, 1:37 PM Hello: I have recently begun to use frozen sections to localize an antigen found in mouse liver tissue. My previous experience has been almost exclusively with staining of formalin-fixed, paraffin-embedded tissues. I have done two runs of this new staining protocol and noticed an odd artifact in both runs. It appears as though there are stringy stars throughout the sections! ?The 'strings' seem to originate from the hepatocyte nuclei leading me to believe that what I am seeing is DNA that has escaped the confines of the nucleus and is streaming out over the tissue. ?I did not squish or drag the coverslips onto/over the sections; this is the only thing that I could imagine would make such a mess. Does anyone have any information on the nature of this artifact and how to prevent it? I will be happy to provide my protocol or a picture if this is an odd artifact with which no one has experience. I suspect, however, that this is a 'newbie' mistake so I will refrain from posting any additional information at the present time. Thanks for your help. ---M.T. Pritchard Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 ? phone:? 216.444.8613 fax:? 216.636.1493 ? email:? pritchm@ccf.org ? Lab location: Lerner Research Institute NE4-214 =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Jan 4 14:31:59 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Jan 4 14:33:08 2009 Subject: [Histonet] colon resection fixation In-Reply-To: Message-ID: <82372DAACDBE410EB1E70C0EACB6F4CD@HPPav2> Another option: When you fix overnight, is that with the colon opened and in fixative, or with the tissue in cassettes in the fixative overnight? That should be fine. Or is that placing the unfixed colon intact into the fixative overnight? If it is this way, then the fixative has not penetrated to the center of the colon, so there is poorly fixation. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph N. Pastore Sent: Saturday, January 03, 2009 7:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] colon resection fixation On our last HistoQuip it was mentioned that our section for a colon specimen was under fixed or poorly fixed. Now ...we routinely fix in formalin overnite and the overnite in O-Fix. But there are times when the specimen has to be stored in the OR refrig after COB. Can this be the problem and does anyone have ideas as to how this can be avoided? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From randek <@t> histopathconcepts.com Sun Jan 4 19:50:15 2009 From: randek <@t> histopathconcepts.com (Rande Kline) Date: Sun Jan 4 19:51:14 2009 Subject: [Histonet] pH strips In-Reply-To: <898015.6031.qm@web65716.mail.ac4.yahoo.com> References: <03C921A1EAF7F541B16543F6EC6A4B37021832EF@giamail2.Gia.com> <898015.6031.qm@web65716.mail.ac4.yahoo.com> Message-ID: I used to be in technical support for a chemical company EMD Chemicals which manufactures pH test strips. If I remember correctly, they were not expiration dated. There are conditions that can cause errors. I attached a brochure that states the errors on page 6. You should keep it for you records. http://www.emdchemicals.com/analytics/literature/colorpHast_Products_for_pH_Testing.pdf Actually, they are pretty accurate. On Wed, Dec 31, 2008 at 3:11 PM, Rene J Buesa wrote: > pH strips are made of a "filter paper like" base paper with pH reacting > reagents for certain pH ranges and, yes, they react improperly after the > date set by the manufacturer. > Ren? J. > > --- On Wed, 12/31/08, Amber McKenzie > wrote: > > From: Amber McKenzie > Subject: [Histonet] pH strips > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, December 31, 2008, 1:43 PM > > Do pH strips go bad after a certain time period? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Rande Kline, HT (ASCP) Histopathology Lab Concepts P-609-744-0803 F-609-939-0270 randek@histopathconcepts.com From Courtney.Cain <@t> pbrc.edu Sun Jan 4 20:55:01 2009 From: Courtney.Cain <@t> pbrc.edu (Courtney Cain) Date: Sun Jan 4 20:55:08 2009 Subject: [Histonet] Using Isopropyl alcohol in tissue processor Message-ID: I was wondering if anyone has tried Isopropyl Alcohol (2-propanol) as an alternative to xylene substitutes in their tissue processors. We are attempting this method with the Thermo Excelsior to process mouse embryo, placenta, liver, and mouse and rat organs. Currently our animal tissue has been immersion fixed, but we have encountered extremely brittle and poor morphology of placenta and livers. A few livers and kidneys have pulled away from the wax as well. Here is our basic protocol that has been attempted with and without vacuum. Formaldehyde 2 hr Formaldehyde 2 hr Isopropyl Alcohol 70% 20min Isopropyl Alcohol 90% 20min Isopropyl Alcohol 95% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Wax 30min Wax 30min Wax 30min Thanks, Courtney Cain Research Associate Cell Biology & Bioimaging Core Pennington Biomedical Research Center 225-763-2653 caincm@pbrc.edu From slappycraw <@t> yahoo.com Mon Jan 5 09:03:49 2009 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Jan 5 09:04:47 2009 Subject: [Histonet] CD45R on Rat tissue Message-ID: <157589.44470.qm@web53607.mail.re2.yahoo.com> Any protocol info for this would be helpful. Thanks in advance. ? Larry A. Woody Seattle, Wa. From rjbuesa <@t> yahoo.com Mon Jan 5 09:13:41 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 5 09:13:45 2009 Subject: [Histonet] Using Isopropyl alcohol in tissue processor In-Reply-To: Message-ID: <970626.67449.qm@web65716.mail.ac4.yahoo.com> Iso-propanol (2-propanol) is the most secure, direct and cheapest alternative to xylene. The only thing I will recommend you is to have the first paraffin bath made of paraffin and 2-propanol at equal amounts in order to reduce the solubility gradient between the last 2-propanol and the first paraffin steps from 9 to 4.5 Mega Pascals. Other than that you will not only eliminate xylene but will reduce costs since 2-propanol is cheaper than ethanol and xylene. Most of the Peloris tissue processors users?rely on?2-propanol as both dehydrating and "ante medium" agents. To clean the tissue processor instead of xylene use a 2% aqueousus v/v solution of a laboratory strength dish washer soap, and you are set to have a tissue processing schedule free from xylene.Ren? J. --- On Sun, 1/4/09, Courtney Cain wrote: From: Courtney Cain Subject: [Histonet] Using Isopropyl alcohol in tissue processor To: histonet@lists.utsouthwestern.edu Date: Sunday, January 4, 2009, 9:55 PM I was wondering if anyone has tried Isopropyl Alcohol (2-propanol) as an alternative to xylene substitutes in their tissue processors. We are attempting this method with the Thermo Excelsior to process mouse embryo, placenta, liver, and mouse and rat organs. Currently our animal tissue has been immersion fixed, but we have encountered extremely brittle and poor morphology of placenta and livers. A few livers and kidneys have pulled away from the wax as well. Here is our basic protocol that has been attempted with and without vacuum. Formaldehyde 2 hr Formaldehyde 2 hr Isopropyl Alcohol 70% 20min Isopropyl Alcohol 90% 20min Isopropyl Alcohol 95% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Wax 30min Wax 30min Wax 30min Thanks, Courtney Cain Research Associate Cell Biology & Bioimaging Core Pennington Biomedical Research Center 225-763-2653 caincm@pbrc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Mon Jan 5 10:21:41 2009 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Jan 5 10:23:36 2009 Subject: [Histonet] FW: fetal demise under 20 wks Message-ID: <938D716CD445614ABBB817517557B6F43D1FE497@NADCWPMSGCMS09.hca.corpad.net> Are there any facilities that do not perform a gross and micro on a fetus less than 20 wks, if mom wishes to bury it? Do you have a policy that states this and are you willing to share? Does mom sign a form stating that she does not wish for pathology to be done? Thanks in advance. Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? From RSRICHMOND <@t> aol.com Mon Jan 5 12:41:00 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Jan 5 12:41:07 2009 Subject: [Histonet] Re: fetal demise under 20 wks Message-ID: Jessica Vacca in Brandon FL asks: >>Are there any facilities that do not perform a gross and micro on a fetus less than 20 wks, if mom wishes to bury it? Do you have a policy that states this and are you willing to share? Does mom sign a form stating that she does not wish for pathology to be done?<< In a situation like this, the placenta needs to be examined both grossly and microscopically, but the fetus generally does not. Most pathology labs are not equipped to do an autopsy on a fetus this size, and most pathologists (definitely including me) are not trained to do this kind of autopsy. Most of the services I've worked on do not routinely dissect the fetus. Certainly, before I'd undertake any such autopsy, I'd want to make sure that a funeral wasn't planned. Believe or not, open casket funerals for fetuses are not unusual. By the way, if you do dispose of a fetus, do so with great care, and make sure that all identification is removed from it before final disposal. Anti-abortion crazies have sometimes used this information to harass the bereaved mother, as if she'd had the pregnancy aborted. (This isn't an urban legend - I can supply a reference.) Bob Richmond Samurai Pathologist Knoxville TN From drvet_anjan <@t> hotmail.com Mon Jan 5 12:43:56 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Mon Jan 5 12:44:02 2009 Subject: [Histonet] mountants - organic/ aqueous Message-ID: hi, i wanted to know what is the difference between organic mountants / aqueous mountants / permanent mountants, which is used in immunohistochemistry. can anyone plz illustrate with examples. i also wanted to know what kind of mountants are used for diffeent kinds of chromogen like DAB, fast red, bajoran purple, fuchsin, fast blue.,....... regards, anjan _________________________________________________________________ Find a better job. We have plenty. Visit MSN Jobs http://www.in.msn.com/jobs From jshelley <@t> burnham.org Mon Jan 5 12:45:25 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Mon Jan 5 12:46:03 2009 Subject: [Histonet] RE: Using Isopropyl alcohol in tissue processor (Courtney Cain) In-Reply-To: <52dd880e-414f-415a-9848-a063a3462ba5@highlander.burnham.org> References: <52dd880e-414f-415a-9848-a063a3462ba5@highlander.burnham.org> Message-ID: Hi Courtney, I was wondering at what temperature you have your wax at because I think it could be that all the alcohol is not clearing out of the tissue and that is why you are getting some of that separation. Do you ever smell alcohol in the tissue that explode like you do when xylene has not completely cleared out. I am also working with the Excelsior and Isopropyl alcohol and found that my alcohol times needed to be a bit longer and the wax should probably be double to what you are doing now. You can email me and I can give you my protocols. John Shelley Histology Core Facility Burnham Institute for Medical Research 8669 Commodity Circle Orlando, FL 32819 email:jshelley@burnham.org Message: 5 Date: Sun, 4 Jan 2009 20:55:01 -0600 From: "Courtney Cain" Subject: [Histonet] Using Isopropyl alcohol in tissue processor To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I was wondering if anyone has tried Isopropyl Alcohol (2-propanol) as an alternative to xylene substitutes in their tissue processors. We are attempting this method with the Thermo Excelsior to process mouse embryo, placenta, liver, and mouse and rat organs. Currently our animal tissue has been immersion fixed, but we have encountered extremely brittle and poor morphology of placenta and livers. A few livers and kidneys have pulled away from the wax as well. Here is our basic protocol that has been attempted with and without vacuum. Formaldehyde 2 hr Formaldehyde 2 hr Isopropyl Alcohol 70% 20min Isopropyl Alcohol 90% 20min Isopropyl Alcohol 95% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Wax 30min Wax 30min Wax 30min Thanks, Courtney Cain Research Associate Cell Biology & Bioimaging Core Pennington Biomedical Research Center 225-763-2653 caincm@pbrc.edu Message: 7 Date: Mon, 5 Jan 2009 07:13:41 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Using Isopropyl alcohol in tissue processor To: histonet@lists.utsouthwestern.edu, Courtney Cain Message-ID: <970626.67449.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Iso-propanol (2-propanol) is the most secure, direct and cheapest alternative to xylene. The only thing I will recommend you is to have the first paraffin bath made of paraffin and 2-propanol at equal amounts in order to reduce the solubility gradient between the last 2-propanol and the first paraffin steps from 9 to 4.5 Mega Pascals. Other than that you will not only eliminate xylene but will reduce costs since 2-propanol is cheaper than ethanol and xylene. Most of the Peloris tissue processors users?rely on?2-propanol as both dehydrating and "ante medium" agents. To clean the tissue processor instead of xylene use a 2% aqueousus v/v solution of a laboratory strength dish washer soap, and you are set to have a tissue processing schedule free from xylene.Ren? J. --- On Sun, 1/4/09, Courtney Cain wrote: From: Courtney Cain Subject: [Histonet] Using Isopropyl alcohol in tissue processor To: histonet@lists.utsouthwestern.edu Date: Sunday, January 4, 2009, 9:55 PM I was wondering if anyone has tried Isopropyl Alcohol (2-propanol) as an alternative to xylene substitutes in their tissue processors. We are attempting this method with the Thermo Excelsior to process mouse embryo, placenta, liver, and mouse and rat organs. Currently our animal tissue has been immersion fixed, but we have encountered extremely brittle and poor morphology of placenta and livers. A few livers and kidneys have pulled away from the wax as well. Here is our basic protocol that has been attempted with and without vacuum. Formaldehyde 2 hr Formaldehyde 2 hr Isopropyl Alcohol 70% 20min Isopropyl Alcohol 90% 20min Isopropyl Alcohol 95% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Isopropyl Alcohol100% 20min Wax 30min Wax 30min Wax 30min Thanks, Courtney Cain Research Associate Cell Biology & Bioimaging Core Pennington Biomedical Research Center 225-763-2653 caincm@pbrc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 5 *************************************** From gamal.akabani <@t> gmail.com Mon Jan 5 12:54:59 2009 From: gamal.akabani <@t> gmail.com (Gamal Akabani) Date: Mon Jan 5 12:55:03 2009 Subject: [Histonet] Need help on understanding the pro and cons of a Leica CM3600 XP crymacrotome. Message-ID: Hello there: I am researching the Leica CM3600 XP cryomacrotome for a small-animal imaging research facility and I would like to know who has experience with this machine. I thank you in advanced for all the help I can get. Gamal Akabani From billodonnell <@t> catholichealth.net Mon Jan 5 12:58:07 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Mon Jan 5 12:59:21 2009 Subject: [Histonet] Re: fetal demise under 20 wks In-Reply-To: References: Message-ID: Concerning disposal. Many (most) funeral homes are willing to do a cremation of remains, remaining respectful of the remains and keeping the consciences of any that might be sensitive in these matters clear. They will likely do so in "groups". I'll be refraining from using inflamatory remarks concerning those who both oppose and support abortion rights by less-than-orthodox methods. It twould be good to see the same in future posts. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 05, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: fetal demise under 20 wks Jessica Vacca in Brandon FL asks: >>Are there any facilities that do not perform a gross and micro on a fetus less than 20 wks, if mom wishes to bury it? Do you have a policy that states this and are you willing to share? Does mom sign a form stating that she does not wish for pathology to be done?<< In a situation like this, the placenta needs to be examined both grossly and microscopically, but the fetus generally does not. Most pathology labs are not equipped to do an autopsy on a fetus this size, and most pathologists (definitely including me) are not trained to do this kind of autopsy. Most of the services I've worked on do not routinely dissect the fetus. Certainly, before I'd undertake any such autopsy, I'd want to make sure that a funeral wasn't planned. Believe or not, open casket funerals for fetuses are not unusual. By the way, if you do dispose of a fetus, do so with great care, and make sure that all identification is removed from it before final disposal. Anti-abortion crazies have sometimes used this information to harass the bereaved mother, as if she'd had the pregnancy aborted. (This isn't an urban legend - I can supply a reference.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Jan 5 13:14:01 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Jan 5 13:15:23 2009 Subject: [Histonet] Re: fetal demise under 20 wks In-Reply-To: Message-ID: You local chapter of Catholic Charities will collect the fetuses and provide burial - regardless of where they come from. "O'Donnell, Bill" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/05/2009 12:58 PM To "Robert Richmond" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Re: fetal demise under 20 wks Concerning disposal. Many (most) funeral homes are willing to do a cremation of remains, remaining respectful of the remains and keeping the consciences of any that might be sensitive in these matters clear. They will likely do so in "groups". I'll be refraining from using inflamatory remarks concerning those who both oppose and support abortion rights by less-than-orthodox methods. It twould be good to see the same in future posts. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 05, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: fetal demise under 20 wks Jessica Vacca in Brandon FL asks: >>Are there any facilities that do not perform a gross and micro on a fetus less than 20 wks, if mom wishes to bury it? Do you have a policy that states this and are you willing to share? Does mom sign a form stating that she does not wish for pathology to be done?<< In a situation like this, the placenta needs to be examined both grossly and microscopically, but the fetus generally does not. Most pathology labs are not equipped to do an autopsy on a fetus this size, and most pathologists (definitely including me) are not trained to do this kind of autopsy. Most of the services I've worked on do not routinely dissect the fetus. Certainly, before I'd undertake any such autopsy, I'd want to make sure that a funeral wasn't planned. Believe or not, open casket funerals for fetuses are not unusual. By the way, if you do dispose of a fetus, do so with great care, and make sure that all identification is removed from it before final disposal. Anti-abortion crazies have sometimes used this information to harass the bereaved mother, as if she'd had the pregnancy aborted. (This isn't an urban legend - I can supply a reference.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Mon Jan 5 13:32:31 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Jan 5 13:32:49 2009 Subject: [Histonet] Re: fetal demise under 20 wks In-Reply-To: References: Message-ID: <5b6eb13e0901051132p13b99b9ahdb90e02531ac17db@mail.gmail.com> Most places I've worked usually put a piece of placenta through processing and let the fetus go to the funeral home if no autopsy is performed and the family plans a funeral. There was one lab that would just measure the footlength and do a gross description, but that was for planned abortions. "Anti-abortion crazies" is an accurate and non-inflammatory description of someone who steals confidential information and then tries to harrass a patient. Protecting patient's personal information is the law. Stealing info and harassing people is against the law. Mark On 1/5/09, O'Donnell, Bill wrote: > > Concerning disposal. Many (most) funeral homes are willing to do a > cremation of remains, remaining respectful of the remains and keeping > the consciences of any that might be sensitive in these matters clear. > They will likely do so in "groups". > > I'll be refraining from using inflamatory remarks concerning those who > both oppose and support abortion rights by less-than-orthodox methods. > It twould be good to see the same in future posts. > > William (Bill) O'Donnell, HT (ASCP) QIHC > Lead Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > Sent: Monday, January 05, 2009 12:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: fetal demise under 20 wks > > Jessica Vacca in Brandon FL asks: >>Are there any facilities that do not > perform a gross and micro on a fetus less than 20 wks, if mom wishes to > bury it? Do you have a policy that states this and are you willing to > share? Does mom sign a form stating that she does not wish for pathology > to be done?<< > > In a situation like this, the placenta needs to be examined both grossly > and microscopically, but the fetus generally does not. Most pathology > labs are not equipped to do an autopsy on a fetus this size, and most > pathologists (definitely including me) are not trained to do this kind > of autopsy. Most of the services I've worked on do not routinely dissect > the fetus. > > Certainly, before I'd undertake any such autopsy, I'd want to make sure > that a funeral wasn't planned. Believe or not, open casket funerals for > fetuses are not unusual. > > By the way, if you do dispose of a fetus, do so with great care, and > make sure that all identification is removed from it before final > disposal. Anti-abortion crazies have sometimes used this information to > harass the bereaved mother, as if she'd had the pregnancy aborted. > (This isn't an urban legend - I can supply a reference.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Charles.Embrey <@t> carle.com Mon Jan 5 15:03:58 2009 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Jan 5 15:05:02 2009 Subject: [Histonet] Re: fetal demise under 20 wks In-Reply-To: References: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE625@EXCHANGEBE1.carle.com> The state of Illinois actually has a law concerning this situation. Any miscarriage less than 20 weeks requires a state form be given to the mother asking if she would like the remains handled as a surgical specimen and thus disposed of as a surgical specimen or handled as a fetal death requiring funeral home burial or cremation at the parent's expense. This law rose from a situation in which a nurse miscarried and told hospital staff she wanted her baby released to the funeral home after pathology examination. The next week when the funeral home inquired of picking up the baby it couldn't be found and was discovered to have been discarded as hospital waste. There was a lawsuit and legislative involvement that led to this law. If the mother signs requesting the funeral home I can only touch the baby with a full autopsy authorization permit. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, January 05, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: fetal demise under 20 wks Jessica Vacca in Brandon FL asks: >>Are there any facilities that do not perform a gross and micro on a fetus less than 20 wks, if mom wishes to bury it? Do you have a policy that states this and are you willing to share? Does mom sign a form stating that she does not wish for pathology to be done?<< In a situation like this, the placenta needs to be examined both grossly and microscopically, but the fetus generally does not. Most pathology labs are not equipped to do an autopsy on a fetus this size, and most pathologists (definitely including me) are not trained to do this kind of autopsy. Most of the services I've worked on do not routinely dissect the fetus. Certainly, before I'd undertake any such autopsy, I'd want to make sure that a funeral wasn't planned. Believe or not, open casket funerals for fetuses are not unusual. By the way, if you do dispose of a fetus, do so with great care, and make sure that all identification is removed from it before final disposal. Anti-abortion crazies have sometimes used this information to harass the bereaved mother, as if she'd had the pregnancy aborted. (This isn't an urban legend - I can supply a reference.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mweirauch <@t> crittenton.com Mon Jan 5 15:37:46 2009 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Mon Jan 5 15:40:21 2009 Subject: [Histonet] Recycling alcohol, xylene and formalin Message-ID: Hi histonetters, We are looking at the possibility of recycling alcohol, xylene and formalin at our hospital. We currently waste haul the used xylene, and are allowed (at our quantity) to drain dispose the alcohol and formalin. Would anyone who is recycling these products be willing to share their pros and cons? Thanks in advance! Maray From cforster <@t> umn.edu Mon Jan 5 15:45:37 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jan 5 15:46:24 2009 Subject: [Histonet] help with muscle histology Message-ID: <49627F81.1030201@umn.edu> Fellow Histonetters...any help would be appreciated. I have a couple questions about getting good muscle samples. I have a project in the lab right now that is working with laser. The student is taking rat legs for this. What he does is cut a laser line into the large muscle of the rat lag at different settings. Then he sacrifices the rat and harvests the entire leg. He fixes this (this time fro 3 weeks) so I know it is fixed and then cuts the sample just before and after the laser cut. Here are the tweo problems I need help with: 1. When he does this he leaves the rat femur in the sample. So, I have been decaling this with an EDTA solution. Should I use something else? 2. When I cut these after they have decaled and been processed etc., the muscle seems to separate instead of staying closely bundled. This is a problem as we want to measure the zone of tissues damage from the laser mark. 3. Any help would be appreciated......I thought maybe you could give me would be greatly appreciated!! Thanks in advance..... Colleen Forster U of MN From NLinke <@t> mednet.ucla.edu Mon Jan 5 16:01:44 2009 From: NLinke <@t> mednet.ucla.edu (Linke, Noelle) Date: Mon Jan 5 16:02:42 2009 Subject: [Histonet] Sakura XPress Message-ID: <0C96F0BFE078D74C91A1C541D24A6AE43A4A1F3E@EMGMB1.ad.medctr.ucla.edu> Hi everyone, I'm looking for folks who are using the Tissue Tek Xpress in their labs. To what extent are you using it? What are some of the technical problems you are running into? Thank you! Noelle No?lle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology & Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nlinke@mednet.ucla.edu ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From rjbuesa <@t> yahoo.com Mon Jan 5 16:14:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 5 16:14:11 2009 Subject: [Histonet] Recycling alcohol, xylene and formalin In-Reply-To: Message-ID: <576473.14007.qm@web65716.mail.ac4.yahoo.com> Maray: I am sure that you will get some advise from the colleagues, but I think that you will better off if you go to HistoNet archives where these recycling issues have been discussed previously in extenso. I am quite surprised that your county allows sink disposal of formalin, I don't think that they have looked carefully into this issue. Ren? J. --- On Mon, 1/5/09, Maray Weirauch wrote: From: Maray Weirauch Subject: [Histonet] Recycling alcohol, xylene and formalin To: Histonet@lists.utsouthwestern.edu Date: Monday, January 5, 2009, 4:37 PM Hi histonetters, We are looking at the possibility of recycling alcohol, xylene and formalin at our hospital. We currently waste haul the used xylene, and are allowed (at our quantity) to drain dispose the alcohol and formalin. Would anyone who is recycling these products be willing to share their pros and cons? Thanks in advance! Maray _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Mon Jan 5 16:43:30 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Jan 5 16:44:31 2009 Subject: [Histonet] RE: Differences between WB antibody and IHC antibody In-Reply-To: References: <200812301644573521912@foxmail.com> <200812310325559009046@foxmail.com> <200901022114549441081@foxmail.com> Message-ID: When I'm trying a new antibody for IHC, I will typically test several concentrations of the antibody and ALSO several different antigen retrieval methods side-by side. I typically do Heat Induced Epitope retrieval at pH 6.0 and 9.0, and also trypsin digestion for 30 minutes. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 2 Jan 2009 17:14:12 -0500 > From: anh2006@med.cornell.edu > To: talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu; tifei@foxmail.com > Subject: Re: [Histonet] Differences between WB antibody and IHC antibody > CC: > > Whether or not an antibody can be used for western vs IHC depends on > many factors. > > Some antibodies can be used for both, some can be only used for one > vs another, and some cannot be used for either. > > There are quite a few factors to consider and here are a few from the > top of my head (and I am hoping people can add to this list): > > (1) Epitope recognition > > Western blotting often times (but not always of course) involves the > denaturation of proteins as well as breaking of disulfide bonds etc. > Samples are often boiled in denaturing agents such as > beta-mercaptoethanol or DTT and are run in SDS containing buffers and > gels. In doing this certain epitopes may be revealed enabling a > specific antibody to work on western vs IHC. Along those same lines, > this denaturation of proteins can also destroy epitopes, because > proteins are no longer in their native conformations, disabling the > efficacy of certain antibodies. > > Similarly, IHC involves fixation, processing, sectioning, heat > retrieval etc. Fixation alone can alter epitopes dramatically thereby > precventing a certain antibody from binding. On the contrary, > proteins are often well preserved in a native conformation in IHC > (especially in fresh frozen sections). > > (2) Access > > Because western blotting involves protein lysates of cells which have > been busted open by a variety of methods, all of the proteins in a > cell should be accessible to the antibody. In IHC on sections or on > coverslips etc, the cell structures are largely preserved intact. > Therefore certain cell compartments may need further processing to > grant access of the big bulky antibody to the inside of the > compartments. Hence part of the reason why we do "antigen retrieval" > with enzymes and detergents etc. Similarly because in western, one > might spin out fractions before running on a gel if you are searching > for a nuclear protein or other protein localized to a certain > compartment you must be sure you actually have the compartment > preserved in a western prep where you always would have it present in > an intact IHC section. > > (3) Sensitivity > > Western blotting is superior to run-of-the-mill IHC in detection > sensitivity. This is even more obvious when one considers how easy it > is to interpret weak or sparse IHC staining as background. Background > is much easier to interpret in a western blot. Therefore an antibody > which works beautifully in WB may work in IHC but the proteins might > be too sparse or too few in number to be visualized. > > (4) Polyclonal vs. monoclonal > > If an antibody is a polyclonal antibody (pAb) it will likely have a > better chance to work across a broad spectrum of applications. This > is because a polyclonal antibody preparation is actually a > combination of different Ig's recognizing more than one epitope > (although it is important to know that there may be a predominant Ig > present or the pAb could have been purified in a way to enrich for a > particular antigen which may alter it's ability to work in a broad > range of apps). > > That's all I can think of for now, and I hope this makes sense ... > but as someone else said, it's all empirical and has to be tested in > each person's lab. > > Happy New Year! > > > >If anyone replies with posting to the list, please forward it. I've always > >wondered the same thing. > > > >On Fri, Jan 2, 2009 at 8:15 AM, TF wrote: > > > > > Hi > > > Just wondering whether the antibody for WB (only WB, IP on datasheet) can > >> be used for IHC use? > >> If not, why? > >> > >> Can I just increase the concentration for IHC application? > >> > >> I want to learn more about the underlying production processes. > >> Thanks! > >> > >> > >> 2009-01-02 > >> > >> > >> > > > TF > > -- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ It?s the same Hotmail?. If by ?same? you mean up to 70% faster. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_broad1_122008 From nefff <@t> staff.uni-marburg.de Tue Jan 6 03:36:56 2009 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Tue Jan 6 03:37:07 2009 Subject: [Histonet] EvG restain Message-ID: <1231234616.496326380a71d@webmail.med.uni-marburg.de> Dear netters, I've got few slides stained with EvG but the red "collagen"-stain (I think its the "s?urefuchsin" step) is very weak (But there are arteries and meningial tissue that should display collagen). Does anyone know, if it is possible to destain these EvG slides and restain them with EvG or just the "s?urefuchsin" step to intensify the collagen stain? Thanks in advance, Frauke ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From gu.lang <@t> gmx.at Tue Jan 6 06:27:05 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 6 06:28:08 2009 Subject: AW: [Histonet] EvG restain In-Reply-To: <1231234616.496326380a71d@webmail.med.uni-marburg.de> References: <1231234616.496326380a71d@webmail.med.uni-marburg.de> Message-ID: I think it's possible. But I wouldn't destain it, just go back through xylene and ethanols to water. Then check in the microscope, if the elastic fibers are still well stained. If they are put the slides again in the vanGieson solution (=pikrofuchsin, =s?urefuchsin in satured picric acid) for 3-5 min. then short differentiating in 96% ethanol, then dehydrating, clearing and coverslipping. VanGieson tends to fade out with time. Bye und tsch?s Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Dr. med. Frauke Neff Gesendet: Dienstag, 06. J?nner 2009 10:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] EvG restain Dear netters, I've got few slides stained with EvG but the red "collagen"-stain (I think its the "s?urefuchsin" step) is very weak (But there are arteries and meningial tissue that should display collagen). Does anyone know, if it is possible to destain these EvG slides and restain them with EvG or just the "s?urefuchsin" step to intensify the collagen stain? Thanks in advance, Frauke ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Tue Jan 6 09:00:02 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Jan 6 09:01:04 2009 Subject: [Histonet] decal solutions Message-ID: <62A8156F8071C8439080D626DF8C33A602E5D4@wave-mail.7thwave.local> Can anyone recommend a decalcifying solution that works well for large animal (dogs, pigs, etc.) bones like femurs and sternums? Thanks in advance for any input. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From tbraud <@t> holyredeemer.com Tue Jan 6 11:29:24 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 6 11:29:40 2009 Subject: [Histonet] H-QIP Message-ID: I just reviewed the 2008 H-QIP B Final Critique with my staff. For want of a more practical discussion, their one overwhelming response was, "GET REAL!!!" Case in point and I quote, "Fixation - the spleen should be weighed, measured, sliced into thin sections, laid out, and placed into a large among of fixative as soon as they are brought to the laboratory." In a busy working lab, few have the luxury of staff and time to perform the steps necessary for the perfection of fixation and processing that these surveys seem to desire. Also, frequently, a histotech has no input or control over the initial fixation step. Histotechs often process a wide variety of tissues together, and for the sake of turnaround time, not all tissues are processed optimally, but NEVER suboptimal to the point of compromising an accurate diagnosis. I see the benefit of the H-QIP discussion on how to achieve perfectly processed and sectioned tissue, but often, the survey results and resulting followup are forwarded to people outside of laboratory medicine. Those outside of Lab Medicine don't understand the subtle, qualitative difference between "readable" and "perfect", and I, for one, would appreciate that difference included in the Final Critique on H-QIP. There, I feel better now. Thank you for listening. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Tue Jan 6 11:12:52 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 6 11:40:30 2009 Subject: [Histonet] Question to all my colleagues! Message-ID: <83954.38049.qm@web65716.mail.ac4.yahoo.com> First, Happy New Year to all! ? Second: I am sure you are becoming aware of a growing tendency in tissue processing, namely, the increasing use of iso-propanol, also called isopropylic alcohol or 2-propanol for tissue processing in substitution of both ethanol and specially xylene. ? Most of those labs using the Peloris tissue processor use iso-propanol and paraffin wax only, but the use is beginning to spread amongst labs that do not work with this instrument. ? My question is: Would you mind letting me know which laboratories use iso-propanol for tissue processing instead of xylene? ? I will really appreciate your answer, either directly to me or shared with all in HistoNet. Regards Ren? J. From rjbuesa <@t> yahoo.com Tue Jan 6 11:46:44 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 6 11:46:47 2009 Subject: [Histonet] decal solutions In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E5D4@wave-mail.7thwave.local> Message-ID: <468215.76073.qm@web65703.mail.ac4.yahoo.com> The brand name "RDO" Ren? J. --- On Tue, 1/6/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] decal solutions To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 6, 2009, 10:00 AM Can anyone recommend a decalcifying solution that works well for large animal (dogs, pigs, etc.) bones like femurs and sternums? Thanks in advance for any input. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 6 12:03:27 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 6 12:04:24 2009 Subject: [Histonet] H-QIP In-Reply-To: Message-ID: <366389.53402.qm@web65707.mail.ac4.yahoo.com> Dear Terri: Reading your comment I became aware of your frustration, that I share. At the end your "There, I feel better now" sums up your state of mind.? Now let me try to be the Devil's advocate: ? 1- participating in the H-QIP is similar to sending a draft to a journal to be subjected to peer review. The reviewers will write their opinions and the author will address them. Some Journals wrongly think that it is the duty of the author to accept ANY and ALL?comments and?incorporate?them into the article and that is wrong. The author has an insight on the article privy to him or her, and the reviewer sometimes has a strong opinion about something that sometimes do not coincide with that of the author's, so a "compromise" is needed. ? 2- a critique from the H-QIP is similar; the reviewers, as you point out, point out to defects and give?optimal solutions. I personally agree with you that a busy laboratory does not have the "luxury" of treating all tissues "ideally" and the recommendations about the spleen are just that, optimal, but that does not mean tha you should not try improve your method. You could set provisions to, within the actual possibilities, try to improve the ways you fix your tissue. This is a "two ways avenue", the H-QIP reviewers also have to "get real" and you should improve, otherwise, in order not to upset you in the future, just stop sending your slides to H-QIP. I am sure that your slides are perfect?"for diagnostic purposes" even if they are not "histologically perfect". ? There, that is my comment and advise to you! Happy New Year! Ren? J.? --- On Tue, 1/6/09, Terri Braud wrote: From: Terri Braud Subject: [Histonet] H-QIP To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 6, 2009, 12:29 PM I just reviewed the 2008 H-QIP B Final Critique with my staff. For want of a more practical discussion, their one overwhelming response was, "GET REAL!!!" Case in point and I quote, "Fixation - the spleen should be weighed, measured, sliced into thin sections, laid out, and placed into a large among of fixative as soon as they are brought to the laboratory." In a busy working lab, few have the luxury of staff and time to perform the steps necessary for the perfection of fixation and processing that these surveys seem to desire. Also, frequently, a histotech has no input or control over the initial fixation step. Histotechs often process a wide variety of tissues together, and for the sake of turnaround time, not all tissues are processed optimally, but NEVER suboptimal to the point of compromising an accurate diagnosis. I see the benefit of the H-QIP discussion on how to achieve perfectly processed and sectioned tissue, but often, the survey results and resulting followup are forwarded to people outside of laboratory medicine. Those outside of Lab Medicine don't understand the subtle, qualitative difference between "readable" and "perfect", and I, for one, would appreciate that difference included in the Final Critique on H-QIP. There, I feel better now. Thank you for listening. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Jan 6 12:20:16 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jan 6 12:21:26 2009 Subject: [Histonet] H-QIP References: <366389.53402.qm@web65707.mail.ac4.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F287F@fhosxchmb006.ADVENTISTCORP.NET> Dear All, Do you think that eventually H-QIP will enact that we MUST spend the time to do procedures such as sectioning the spleen in preparation for fixation? -Which will in turn enable us to again have adequate staff due to required workload hours? The circle coming full around after 30 years? Janet Janet L. Bonner, HTL (ASCP) Pathology Laboratory ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Tue 1/6/2009 1:03 PM To: histonet@lists.utsouthwestern.edu; Terri Braud Subject: Re: [Histonet] H-QIP Dear Terri: Reading your comment I became aware of your frustration, that I share. At the end your "There, I feel better now" sums up your state of mind. Now let me try to be the Devil's advocate: 1- participating in the H-QIP is similar to sending a draft to a journal to be subjected to peer review. The reviewers will write their opinions and the author will address them. Some Journals wrongly think that it is the duty of the author to accept ANY and ALL comments and incorporate them into the article and that is wrong. The author has an insight on the article privy to him or her, and the reviewer sometimes has a strong opinion about something that sometimes do not coincide with that of the author's, so a "compromise" is needed. 2- a critique from the H-QIP is similar; the reviewers, as you point out, point out to defects and give optimal solutions. I personally agree with you that a busy laboratory does not have the "luxury" of treating all tissues "ideally" and the recommendations about the spleen are just that, optimal, but that does not mean tha you should not try improve your method. You could set provisions to, within the actual possibilities, try to improve the ways you fix your tissue. This is a "two ways avenue", the H-QIP reviewers also have to "get real" and you should improve, otherwise, in order not to upset you in the future, just stop sending your slides to H-QIP. I am sure that your slides are perfect "for diagnostic purposes" even if they are not "histologically perfect". There, that is my comment and advise to you! Happy New Year! Ren? J. --- On Tue, 1/6/09, Terri Braud wrote: From: Terri Braud Subject: [Histonet] H-QIP To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 6, 2009, 12:29 PM I just reviewed the 2008 H-QIP B Final Critique with my staff. For want of a more practical discussion, their one overwhelming response was, "GET REAL!!!" Case in point and I quote, "Fixation - the spleen should be weighed, measured, sliced into thin sections, laid out, and placed into a large among of fixative as soon as they are brought to the laboratory." In a busy working lab, few have the luxury of staff and time to perform the steps necessary for the perfection of fixation and processing that these surveys seem to desire. Also, frequently, a histotech has no input or control over the initial fixation step. Histotechs often process a wide variety of tissues together, and for the sake of turnaround time, not all tissues are processed optimally, but NEVER suboptimal to the point of compromising an accurate diagnosis. I see the benefit of the H-QIP discussion on how to achieve perfectly processed and sectioned tissue, but often, the survey results and resulting followup are forwarded to people outside of laboratory medicine. Those outside of Lab Medicine don't understand the subtle, qualitative difference between "readable" and "perfect", and I, for one, would appreciate that difference included in the Final Critique on H-QIP. There, I feel better now. Thank you for listening. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Jackie.O'Connor <@t> abbott.com Tue Jan 6 12:42:20 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jan 6 12:43:45 2009 Subject: [Histonet] H-QIP - spleens In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F287F@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: OK - my question is - - what in the heck do you need to examine the ENTIRE spleen for that would require breadloafing the whole thing for fixation? I was in hospital histology for a couple hundred years, and as I recall in my feeble, senility- prone years, most of the spleens that made it to pathology were from trauma. The only time I've ever seen pathology in a spleen was when my dog had a primary splenic tumor (he died). Is there some new anomaly that happens in spleens these days that require the entire spleen to be examined? Just curious. From tbritten <@t> aol.com Tue Jan 6 12:41:31 2009 From: tbritten <@t> aol.com (tbritten) Date: Tue Jan 6 12:46:06 2009 Subject: [Histonet] used hacker bright cryostat... Message-ID: <8c167f2d.e007.4fd4.ae00.86533cf05772@aol.com> hello all; we are looking to purchase the above. if you have one around the lab not being used maybe this is a way to make some $ for another piece of equipment? please contact my colleague (charlie snyder 973 625-8822 x43) or above email if you wish. thanks and best regards, tom britten From gu.lang <@t> gmx.at Tue Jan 6 13:05:58 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 6 13:06:11 2009 Subject: AW: [Histonet] H-QIP In-Reply-To: References: Message-ID: What I have to add is, that such quality-assessments bring also the MDs and managers in contact with technical questions. Sometimes these organisations are the helping hand for the histotechs arguments. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Terri Braud Gesendet: Dienstag, 06. J?nner 2009 18:29 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] H-QIP I just reviewed the 2008 H-QIP B Final Critique with my staff. For want of a more practical discussion, their one overwhelming response was, "GET REAL!!!" Case in point and I quote, "Fixation - the spleen should be weighed, measured, sliced into thin sections, laid out, and placed into a large among of fixative as soon as they are brought to the laboratory." In a busy working lab, few have the luxury of staff and time to perform the steps necessary for the perfection of fixation and processing that these surveys seem to desire. Also, frequently, a histotech has no input or control over the initial fixation step. Histotechs often process a wide variety of tissues together, and for the sake of turnaround time, not all tissues are processed optimally, but NEVER suboptimal to the point of compromising an accurate diagnosis. I see the benefit of the H-QIP discussion on how to achieve perfectly processed and sectioned tissue, but often, the survey results and resulting followup are forwarded to people outside of laboratory medicine. Those outside of Lab Medicine don't understand the subtle, qualitative difference between "readable" and "perfect", and I, for one, would appreciate that difference included in the Final Critique on H-QIP. There, I feel better now. Thank you for listening. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jan 6 13:15:48 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 6 13:16:46 2009 Subject: [Histonet] San Diego Jobs Message-ID: I have a colleague that is looking for histotech jobs in the San Diego area. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From sbreeden <@t> nmda.nmsu.edu Tue Jan 6 13:22:22 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jan 6 13:22:28 2009 Subject: [Histonet] Power Supply for Processor Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6606@nmdamailsvr.nmda.ad.nmsu.edu> As always, I turn to Those Who Know These Things. I am ashamed, but I do not have my processor attached to a surge protector or UPS (uninterruptable power supply with surge protector thingy) - I am suffering pangs of guilt and, at the same time, recovering from this morning's service call to repair a melted power supply. Not wanting to do this again, I'm asking for recommendations on a reasonably-priced (that's all relative, I'm sure) UPS or hefty surge suppressor for my ASP300. I have the info I got from Leica when I took the training, but I would rather not have to install a unit INTO the processor; I prefer an external. Should I pack myself off to Radio Shack or could you provide Serious Moral Guidance. And my middle name is not "Brevity". Adios. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From CHRISH <@t> HEALTHCARESCOUTS.COM Tue Jan 6 13:18:20 2009 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Tue Jan 6 13:28:32 2009 Subject: [Histonet] Immediate Need for Histotech in Fort Myers, FL Message-ID: The #1 Recruiter for Laboratory/Biotech Specialists Healthcare Scouts is a medical laboratory/biotech recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the leading nationally recognized permanent placement solution for medical laboratory/biotech professionals, Healthcare Scouts has the following histology opening in Fort Myers, FL Day shift - 10am to 6pm FL license required this lab offers a comprehensive menu of FISH, Flow, Cytogenetics and Molecular oncology services they pay 100% for health, dental, short term, long term, vision, and life insurance for the employee coverage Please contact me today if you are interested! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From contact <@t> excaliburpathology.com Tue Jan 6 13:43:41 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jan 6 13:43:45 2009 Subject: [Histonet] Power Supply for Processor Message-ID: <915685.7866.qm@web1108.biz.mail.sk1.yahoo.com> I use TigerDirect. Very glad I bought the backup power supplies with surge protection. ________________________________ From: "Breeden, Sara" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 6, 2009 1:22:22 PM Subject: [Histonet] Power Supply for Processor As always, I turn to Those Who Know These Things.? I am ashamed, but I do not have my processor attached to a surge protector or UPS (uninterruptable power supply with surge protector thingy) - I am suffering pangs of guilt and, at the same time, recovering from this morning's service call to repair a melted power supply.? Not wanting to do this again, I'm asking for recommendations on a reasonably-priced (that's all relative, I'm sure) UPS or hefty surge suppressor for my ASP300.? I have the info I got from Leica when I took the training, but I would rather not have to install a unit INTO the processor; I prefer an external.? Should I pack myself off to Radio Shack or could you provide Serious Moral Guidance.? And my middle name is not "Brevity". Adios. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Jan 6 14:50:26 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Jan 6 14:51:24 2009 Subject: [Histonet] IHC price per slide/test Message-ID: <4B78F74F73334426989CBEDFD5A3B6D9@auxs.umn.edu> Hello all, I'm trying to find out the range of IHC fees from 'human' hospitals, but am having difficulty accessing this information from hospital websites (I don't have the time to be put on hold while I call around). I know that veterinary hospitals cannot charge as much for their tests as human hospitals do (no insurance coverage by patients, for instance), but I'd like to find out how wide the disparity is between what you charge and what we charge. We use the same instrumentation and reagents as you (autostainers, same monoclonal Abs, polymer detection systems, etc.) So... what do you charge for tissue/tumor antigen IHC? Infectious disease antigen IHC? I'm not looking for a long list of prices.... just an average fee per slide. Thanks in advance for any help you're able to provide. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jan 6 14:07:47 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jan 6 14:52:56 2009 Subject: [Histonet] Power Supply for Processor In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6606@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sara The normal volt amps for the ASP300/ASP300S is 1000VA. It is recommended that you get a surge protector with a line conditioner that will handle 1200 to 1600VA. The 1000VA represents voltage at normal usage but when the heater turns on for the wax or retort than the voltage will go up. Hence we recommend the higher volt amps. It will cover all possible scenarios. If you have an questions just give me a call. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Breeden, Sara" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Power Supply for Processor 01/06/2009 01:22 PM As always, I turn to Those Who Know These Things. I am ashamed, but I do not have my processor attached to a surge protector or UPS (uninterruptable power supply with surge protector thingy) - I am suffering pangs of guilt and, at the same time, recovering from this morning's service call to repair a melted power supply. Not wanting to do this again, I'm asking for recommendations on a reasonably-priced (that's all relative, I'm sure) UPS or hefty surge suppressor for my ASP300. I have the info I got from Leica when I took the training, but I would rather not have to install a unit INTO the processor; I prefer an external. Should I pack myself off to Radio Shack or could you provide Serious Moral Guidance. And my middle name is not "Brevity". Adios. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Shakun.Aswani <@t> acologix.com Tue Jan 6 15:20:14 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Tue Jan 6 15:21:14 2009 Subject: [Histonet] (no subject) Message-ID: <777AB0DE519C8E46A6220E2287C5BAD3019BB863@EXCHANGE.acologix.com> Hi, Has anyone used Burkhardt's solution as fixative? If yes, can you please share the protocol or where to get it ready to use? Thank you Shakun P. Aswani Scientist I, Preclinical Development Acologix, Inc. 3960 Point Eden Way Hayward CA 94545 (510) 512-7231 phone (510) 786-1116 facsimile shakun.aswani@acologix.com From AnthonyH <@t> chw.edu.au Tue Jan 6 15:26:35 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jan 6 15:26:51 2009 Subject: [Histonet] Question to all my colleagues! In-Reply-To: <83954.38049.qm@web65716.mail.ac4.yahoo.com> Message-ID: Rene, We routinely use xylene for processing. We have a organic solvent recycler so the costs of xylene purchase and disposal are small (though one wonders at the cost of running the recycler!). Our urgent core biopsies are microwave processed and use iso-propanol (via the Mega TT). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, 7 January 2009 4:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question to all my colleagues! First, Happy New Year to all! ? Second: I am sure you are becoming aware of a growing tendency in tissue processing, namely, the increasing use of iso-propanol, also called isopropylic alcohol or 2-propanol for tissue processing in substitution of both ethanol and specially xylene. ? Most of those labs using the Peloris tissue processor use iso-propanol and paraffin wax only, but the use is beginning to spread amongst labs that do not work with this instrument. ? My question is: Would you mind letting me know which laboratories use iso-propanol for tissue processing instead of xylene? ? I will really appreciate your answer, either directly to me or shared with all in HistoNet. Regards Ren? J. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From bamoe <@t> gundluth.org Tue Jan 6 16:12:05 2009 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Tue Jan 6 16:12:12 2009 Subject: [Histonet] Histotech opening La Crosse WI Message-ID: Hi all Gundersen Lutheran Medical Center in La Crosse WI currently has an open histotech position. Looking for 1 full-time or 2 part-time to job share a position. Day-shift - Mon - Fri On-call every 6th or 7th week Fast-paced hospital setting --- routine histology, plus special stains, IHC, and frozen sections. Please contact Human Resources at 608-775-4743, or visit our website at www.gundluth.org For more specific questions/answers please contact Pam Richardson (histology manager) at 608-775-4133 or the histology laboratory at 608-775-3157 Hope to have lots of responses soon : - ) Barb Moe Gundersen Lutheran Medical Center La Crosse WI From saby_joseph_a <@t> yahoo.com Tue Jan 6 18:33:00 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Tue Jan 6 18:33:03 2009 Subject: [Histonet] decal solutions References: <62A8156F8071C8439080D626DF8C33A602E5D4@wave-mail.7thwave.local> Message-ID: <953233.37837.qm@web33808.mail.mud.yahoo.com> All- If you are planning on looking at the bone marrow or are going to be evaluating nuclei in the bone, then I would heartily recommend either using 5% formic acid, or, preferably, a buffered formic acid solution.? The buffer can either be sodium formate or sodium citrate (set to a pH of ~2.2-2.4), but in either case, you can be working with a concentration of 20-25% formice acid and still preserve nuclear detail.? This is not as fast as RDO, but depending on what you want to look at, it can definitely be worth the investment in time! Joe Saby, BA HT ________________________________ From: Michele Wich To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 6, 2009 10:00:02 AM Subject: [Histonet] decal solutions Can anyone recommend a decalcifying solution that works well for large animal (dogs, pigs, etc.) bones like femurs and sternums? Thanks in advance for any input. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure.? If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited.? Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Osullivan <@t> med.monash.edu.au Tue Jan 6 19:12:05 2009 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Tue Jan 6 19:12:11 2009 Subject: [Histonet] double immunoenzymatic methods Message-ID: <130.194.114.97.1231290429@my.monash.edu.au> Hi, Have been having trouble getting an antibody to work for double immunofluoresecence (with the confocal microscope), but am able to get it to work on FFPE tissue with DAB black. Does anyone out there have a method I can use that will show colocalisation using enzyme labelling methods, ie which colour choices do you make to show double labelling within the same structure I understand this is not the preferred method. Regards Kim O'Sullivan From Allison_Scott <@t> hchd.tmc.edu Tue Jan 6 20:03:25 2009 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Jan 6 20:03:32 2009 Subject: [Histonet] PI Reports Message-ID: <1872B4A455B7974391609AD8034C79FC082EDD@LBEXCH01.hchd.local> Happy New Year to all. I would like to know what type of PI reports that are done in your labs. I am looking to find more than the 3 I currently do. Frozen section tat, specimen discrepancy and I was monitoring our immuno tat since we send them out. Also how are you doing your frozen section tat. Any examples of reports would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From aevans <@t> wellspan.org Tue Jan 6 21:03:12 2009 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Tue Jan 6 21:07:37 2009 Subject: [Histonet] Histology Opening in York, Pennsylvania. Full time Days Message-ID: Hello Everyone! I just wanted to let everyone know that we are looking for a full-time Histology Technician/Technologist to work days(hours to be discussed at interview). We are located in York, Pennsylvania and we are a non-profit hospital. We are a very fast paced lab that does about 35,000 surgicals a year and that number grows every year. You would be responsible for Embedding, Microtomy, Special Stains, slide check out, equipment maintenance, trouble shooting, ability to multitask is very helpful and a variety of other tasks. We currently use such equipment as Tissue-Tek VIPS, Histos5 Microwave units, Ventana Nexes Special Stainers, Ventana XT Immunochemistry stainers and VIAS imaging system. If you are interested please email me or check out the posting on our website at www.wellspan.org Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From eridana <@t> cox.net Tue Jan 6 22:43:07 2009 From: eridana <@t> cox.net (Donna Harclerode) Date: Tue Jan 6 22:43:37 2009 Subject: [Histonet] Microtome Cutting Safety References: <11A41625-663C-4843-97D5-B7F480CF4A7D@cox.net> Message-ID: <8666573D-4D9E-41E7-9DF5-8A5ED88171BF@cox.net> Begin forwarded message: > From: Donna Harclerode > Date: January 6, 2009 8:27:14 PM PST > To: histonet-request@lists.utsouthwestern.edu > Subject: Microtome Cutting Safety > > Anyone know of a paraffin microtome that you can section WITH the > knife guard in position? I have always used forceps to keep my > fingers safe, but some techs are harder to convince to do this . I > figured if anyone knows they would be on this list. > I tried the Leica RM2255 (both in automated and manual mode) and I > could sort of section a couple small blocks with the guard up, but > it was not going to work with anything except perfect processed > small blocks and not well for those. > > Any opinions on the safety automated versus manual microtomes? I > adore automated cryostats (Leica 3050 is my favorite) , but I can > not figure why use an automated paraffin microtome. Logically I > always figured an automated can do more damage, but really have no > facts. > > Thanks in advance, > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC From abright <@t> brightinstruments.com Wed Jan 7 03:53:50 2009 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Jan 7 04:31:27 2009 Subject: [Histonet] used hacker bright cryostat... In-Reply-To: <8c167f2d.e007.4fd4.ae00.86533cf05772@aol.com> References: <8c167f2d.e007.4fd4.ae00.86533cf05772@aol.com> Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0ACD87@BRIGHT-SBS.Bright.local> Tom, We have a few used Bright OTF/AS models available but they are 230v 50 htz so will not suit your region in you are in the USA, if so hopefully one of our distributors will see your posting. Please take into account that it is very, very favorable to purchase from the UK at present. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tbritten Sent: 06 January 2009 18:42 To: histonet@lists.utsouthwestern.edu Cc: csnyder@scimedx.com Subject: [Histonet] used hacker bright cryostat... hello all; we are looking to purchase the above. if you have one around the lab not being used maybe this is a way to make some $ for another piece of equipment? please contact my colleague (charlie snyder 973 625-8822 x43) or above email if you wish. thanks and best regards, tom britten _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jan 7 06:25:32 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jan 7 06:26:01 2009 Subject: [Histonet] Microtome Cutting Safety In-Reply-To: <8666573D-4D9E-41E7-9DF5-8A5ED88171BF@cox.net> References: <11A41625-663C-4843-97D5-B7F480CF4A7D@cox.net> <8666573D-4D9E-41E7-9DF5-8A5ED88171BF@cox.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402AD20@LTA3VS011.ees.hhs.gov> I use my fingers too, so no help there. But an automated microtome is great because it frees both hands to handle a ribbon as it is coming off the blade. It is easy to get careless if you use a foot pedal but the one time I have been seriously cut it was with a manual microtome so I guess the moral is to simply be careful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Harclerode Sent: Tuesday, January 06, 2009 11:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Cutting Safety Begin forwarded message: > From: Donna Harclerode > Date: January 6, 2009 8:27:14 PM PST > To: histonet-request@lists.utsouthwestern.edu > Subject: Microtome Cutting Safety > > Anyone know of a paraffin microtome that you can section WITH the > knife guard in position? I have always used forceps to keep my fingers > safe, but some techs are harder to convince to do this . I figured if > anyone knows they would be on this list. > I tried the Leica RM2255 (both in automated and manual mode) and I > could sort of section a couple small blocks with the guard up, but it > was not going to work with anything except perfect processed small > blocks and not well for those. > > Any opinions on the safety automated versus manual microtomes? I > adore automated cryostats (Leica 3050 is my favorite) , but I can not > figure why use an automated paraffin microtome. Logically I always > figured an automated can do more damage, but really have no facts. > > Thanks in advance, > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Wed Jan 7 07:27:56 2009 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Wed Jan 7 07:28:55 2009 Subject: [Histonet] Outside Lab Message-ID: Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From jstaruk <@t> masshistology.com Wed Jan 7 09:50:44 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Jan 7 09:50:53 2009 Subject: [Histonet] Outside Lab In-Reply-To: Message-ID: <6B6AD456FE1A4C388C1E194FDFB0DFB8@JimPC> The first question on everybody's mind is "How much are you charging them"? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jan 7 10:02:49 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 7 10:03:02 2009 Subject: [Histonet] Outside Lab In-Reply-To: Message-ID: Go to http://www.cms.hhs.gov/center/asc.asp for more information about Ambulatory Surgical Centers (ASC's) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From b-frederick <@t> northwestern.edu Wed Jan 7 10:11:21 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Jan 7 10:12:39 2009 Subject: [Histonet] Outside Lab In-Reply-To: <6B6AD456FE1A4C388C1E194FDFB0DFB8@JimPC> Message-ID: <000701c970e2$97db9600$d00f7ca5@lurie.northwestern.edu> Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Wed Jan 7 10:21:13 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Jan 7 10:21:51 2009 Subject: [Histonet] IHC Doublestain Message-ID: <49649028.90CE.001A.3@umm.edu> I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From mari.ann.mailhiot <@t> leica-microsystems.com Wed Jan 7 09:32:42 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Wed Jan 7 10:48:15 2009 Subject: [Histonet] Microtome Cutting Safety In-Reply-To: <8666573D-4D9E-41E7-9DF5-8A5ED88171BF@cox.net> Message-ID: Donna Leica makes a microtome series RM2200 that allows you to cut sections with the knife guard up. You will be able to decide whether you would like a rotary microtome or a automated microtome. I see that you like the Leica CM3050. The comparable microtome for paraffin cutting is the RM2255. Your request can be forwarded to a rep in your area if you like. I will need your zip code to do that. If you need any other information you are welcome to contact me here at Leica. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Donna Harclerode Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Microtome Cutting Safety 01/06/2009 10:43 PM Begin forwarded message: > From: Donna Harclerode > Date: January 6, 2009 8:27:14 PM PST > To: histonet-request@lists.utsouthwestern.edu > Subject: Microtome Cutting Safety > > Anyone know of a paraffin microtome that you can section WITH the > knife guard in position? I have always used forceps to keep my > fingers safe, but some techs are harder to convince to do this . I > figured if anyone knows they would be on this list. > I tried the Leica RM2255 (both in automated and manual mode) and I > could sort of section a couple small blocks with the guard up, but > it was not going to work with anything except perfect processed > small blocks and not well for those. > > Any opinions on the safety automated versus manual microtomes? I > adore automated cryostats (Leica 3050 is my favorite) , but I can > not figure why use an automated paraffin microtome. Logically I > always figured an automated can do more damage, but really have no > facts. > > Thanks in advance, > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mpence <@t> grhs.net Wed Jan 7 10:50:49 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 7 10:50:59 2009 Subject: [Histonet] Outside Lab In-Reply-To: <000701c970e2$97db9600$d00f7ca5@lurie.northwestern.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3A24@IS-E2K3.grhs.net> Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will bill for their own tech part and make money from that part of the service which they are not currently making revenue from. I would assume that your pathologist are their own group and they don't care who makes the tech part? This is just business and you will have to make your services and prices competitive enough to make it not profitable for the urology group. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, January 07, 2009 10:11 AM To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Wed Jan 7 11:11:34 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Jan 7 11:11:39 2009 Subject: [Histonet] Outside Lab Message-ID: <915918.43282.qm@web1104.biz.mail.sk1.yahoo.com> The pathologists are probably going to the urology group to read because CLIA certification is LOCATION specific. The urology group may already have CLIA cert. and is splitting the interpretation charges with the pathologist.?By adding its own lab, they will be able to capture both tech and interp charges. ________________________________ From: Mike Pence To: Bernice Frederick ; jstaruk ; "Senn, Amy R" ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 7, 2009 10:50:49 AM Subject: RE: [Histonet] Outside Lab Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will bill for their own tech part and make money from that part of the service which they are not currently making revenue from. I would assume that your pathologist are their own group and they don't care who makes the tech part?? This is just business and you will have to make your services and prices competitive enough to make it not profitable for the urology group. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, January 07, 2009 10:11 AM To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com ? www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out.? If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money.? We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this?? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA? 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jan 7 11:34:40 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 7 11:35:41 2009 Subject: [Histonet] searching antibodies cdk4 and mdm2 Message-ID: Hi all! Can anybody recommand an anti-human-cdk4 and an anti-human-mdm2 antibody for FFPE tissue? We want to stain liposarcomas and use the Benchmark XT. Until now we have purchased cdk4 from NovusBiological (1:10, 60 min with amplifier) and mdm2 from proteintechgroup (1:50, 32 min). But the result are not very convincing. Any help is appreciated! Bye Gudrun From tifei <@t> foxmail.com Wed Jan 7 11:52:45 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Jan 7 11:53:13 2009 Subject: [Histonet] IHC Doublestain References: <49649028.90CE.001A.3@umm.edu> Message-ID: <200901080152402120720@foxmail.com> QW55IGRpZmZlcmVudCBvZiB0aGUgdHdvIGFwcHJvYWNoZXM/DQpJIHRoaW5rIGZvciBtb3N0IGFu dGlnZW4gdGhleSBib3RoIHdvcmsuDQoNCg0KMjAwOS0wMS0wOCANCg0KDQoNClRGIA0KDQoNCg0K t6K8/sjLo7ogS2ltYmVybHkgVHV0dGxlIA0Kt6LLzcqxvOSjuiAyMDA5LTAxLTA4ICAwMDoyNDo1 OSANCsrVvP7Iy6O6IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdSANCrOty82juiAN Ctb3zOKjuiBbSGlzdG9uZXRdIElIQyBEb3VibGVzdGFpbiANCiANCkkgYW0gc3VwcG9zZWQgdG8g dHJ5IGEgIGRvdWJsZXN0YWluIHVzaW5nIHRoZSBEQUtPIGF1dG9zdGFpbmVyIHdoZXJlIG9uZSBv ZiB0aGUgYW50aWJvZGllcyByZXF1aXJlcyBhIFByb3RlaW5hc2UgSyBwcmV0cmVhdG1lbnQgYW5k IHRoZSBvdGhlciBhbnRpYm9keSByZXF1aXJlcyBhbnRpZ2VuIHJldHJpZXZhbCB3aXRoIGhlYXQu ICBTaG91bGQgSSBkbyBib3RoLCBvciB3aWxsIHRoaXMgbm90IHdvcmsgYXQgYWxsPyBUaGFuayB5 b3UNCktpbWJlcmx5IEMuIFR1dHRsZSAgSFQgKEFTQ1ApDQpQYXRob2xvZ3kgQmlvcmVwb3NpdG9y eSBhbmQgUmVzZWFyY2ggQ29yZQ0KVW5pdmVyc2l0eSBvZiBNYXJ5bGFuZCANClJvb20gTkJXNTgs IFVNTUMNCjIyIFMuIEdyZWVuZSBTdA0KQmFsdGltb3JlLCBNRCAyMTIwMQ0KKDQxMCkgMzI4LTU1 MjQNCig0MTApIDMyOC01NTA4IGZheCANCg0KVGhpcyBlLW1haWwgYW5kIGFueSBhY2NvbXBhbnlp bmcgYXR0YWNobWVudHMgbWF5IGJlIHByaXZpbGVnZWQsIGNvbmZpZGVudGlhbCwgY29udGFpbiBw cm90ZWN0ZWQgaGVhbHRoIGluZm9ybWF0aW9uIGFib3V0IGFuIGlkZW50aWZpZWQgcGF0aWVudCBv ciBiZSBvdGhlcndpc2UgcHJvdGVjdGVkIGZyb20gZGlzY2xvc3VyZS4gU3RhdGUgYW5kIGZlZGVy YWwgbGF3IHByb3RlY3QgdGhlIGNvbmZpZGVudGlhbGl0eSBvZiB0aGlzIGluZm9ybWF0aW9uLiBJ ZiB0aGUgcmVhZGVyIG9mIHRoaXMgbWVzc2FnZSBpcyBub3QgdGhlIGludGVuZGVkIHJlY2lwaWVu dDsgeW91IGFyZSBwcm9oaWJpdGVkIGZyb20gdXNpbmcsIGRpc2Nsb3NpbmcsIHJlcHJvZHVjaW5n IG9yIGRpc3RyaWJ1dGluZyB0aGlzIGluZm9ybWF0aW9uOyB5b3Ugc2hvdWxkIGltbWVkaWF0ZWx5 IG5vdGlmeSB0aGUgc2VuZGVyIGJ5IHRlbGVwaG9uZSBvciBlLW1haWwgYW5kIGRlbGV0ZSB0aGlz IGUtbWFpbC4NCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f DQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVk dQ0K From histonetalias <@t> gmail.com Wed Jan 7 12:12:11 2009 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Wed Jan 7 12:12:17 2009 Subject: [Histonet] Outside Lab In-Reply-To: <915918.43282.qm@web1104.biz.mail.sk1.yahoo.com> References: <915918.43282.qm@web1104.biz.mail.sk1.yahoo.com> Message-ID: <4b6c85510901071012q26ebf8eavf52da8684e5f4a91@mail.gmail.com> Get used to this sort of thing. It is happening in urology and derm practices. You can not blame them for keeping it in-house. They are making a profit on it so why not do it yourself? On Wed, Jan 7, 2009 at 12:11 PM, Paula Pierce < contact@excaliburpathology.com> wrote: > The pathologists are probably going to the urology group to read because > CLIA certification is LOCATION specific. The urology group may already have > CLIA cert. and is splitting the interpretation charges with the > pathologist. By adding its own lab, they will be able to capture both tech > and interp charges. > > > > > ________________________________ > From: Mike Pence > To: Bernice Frederick ; jstaruk < > jstaruk@masshistology.com>; "Senn, Amy R" ; > Histonet@lists.utsouthwestern.edu > Sent: Wednesday, January 7, 2009 10:50:49 AM > Subject: RE: [Histonet] Outside Lab > > Why does the hospital pathologist go to the Urology to read and sign out > the cases? > So if I am understanding this correctly, your hospital is right now > getting the tech part of the specimen for processing. Is your > pathologist part of the hospital or are they their own group? The > urology group will bill for their own tech part and make money from that > part of the service which they are not currently making revenue from. I > would assume that your pathologist are their own group and they don't > care who makes the tech part? > > This is just business and you will have to make your services and prices > competitive enough to make it not profitable for the urology group. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice > Frederick > Sent: Wednesday, January 07, 2009 10:11 AM > To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Outside Lab > > > Is the histo lab in the urology office CAP certified? > Bernice > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk > Sent: Wednesday, January 07, 2009 9:51 AM > To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Outside Lab > > The first question on everybody's mind is "How much are you charging > them"? > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, > Amy R > Sent: Wednesday, January 07, 2009 8:28 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Outside Lab > > Hi histonetters, > > > > Hope everyone is having a good week so far... > > > > Our hospital receives specimens from a Urology group on a daily basis. > Our PA grosses the specimens here; us histotechs work our magic, and the > hospital pathologists take the slides directly to the Urology office to > read them and sign them out. If there are any special stains needed, we > do them here in the hospital. > > > > We are being told that the Urology group is now opening its own > histology lab because it's more cost effective. > > Unless it's too early in the morning for us all to function, us > histotechs can't figure out where they're saving money. We are being > told that the Urology office (or any outside dr's office that has its > own histo lab) can bill Medicare (or any insurance co.) differently, > thereby making more money for the practice itself. > > > > Does anyone have any input on this? One of our pathologists is telling > the Urology group NOT to do this because Medicare may change it's > regulations again & need an 'established' lab to complete the work, > instead of an 'up & coming' laboratory, so he's also against the Urology > group setting up it's own lab. > > > > Thanks for everyone's 0.02. > > > > Have a great day!!! > > > > > > > > > > Amy Senn > > Histotech, Histology Laboratory > > Camp Hill, PA 17011 > > > > > > Confidentiality Disclaimer: The information contained in this > communication may be confidential, is intended for the use of the > recipient named above, and may be legally privileged.If the reader of > this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication, or > any of its contents, is strictly prohibited. If you received this > communication in error, please resend this communication to the sender > and delete the original message and any copy of it from your computer > system. Thank You _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From micropathlabs <@t> yahoo.com Wed Jan 7 13:09:25 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Jan 7 13:10:23 2009 Subject: [Histonet] PTEN Protocol Message-ID: <60155.97612.qm@web57802.mail.re3.yahoo.com> Would anyone using?the Dako?concentrate PTEN (clone 6H2.1) on their?Benchmark XT be willing to share their protocol? We are having difficulty with staining. If we get any staining, it's very weak. We've contacted?Dako but they've had no advice. Our next step is contacting Ventana but we thought we'd check with you all first. Any assistance would be appreciated. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From mpence <@t> grhs.net Wed Jan 7 13:21:21 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 7 13:21:28 2009 Subject: [Histonet] Outside Lab In-Reply-To: <4b6c85510901071012q26ebf8eavf52da8684e5f4a91@mail.gmail.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3A26@IS-E2K3.grhs.net> As long as you have someone who is willing to read it! -----Original Message----- From: Histonet Alias [mailto:histonetalias@gmail.com] Sent: Wednesday, January 07, 2009 12:12 PM To: Paula Pierce Cc: Mike Pence; Histonet Subject: Re: [Histonet] Outside Lab Get used to this sort of thing. It is happening in urology and derm practices. You can not blame them for keeping it in-house. They are making a profit on it so why not do it yourself? On Wed, Jan 7, 2009 at 12:11 PM, Paula Pierce wrote: The pathologists are probably going to the urology group to read because CLIA certification is LOCATION specific. The urology group may already have CLIA cert. and is splitting the interpretation charges with the pathologist. By adding its own lab, they will be able to capture both tech and interp charges. ________________________________ From: Mike Pence To: Bernice Frederick ; jstaruk ; "Senn, Amy R" ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 7, 2009 10:50:49 AM Subject: RE: [Histonet] Outside Lab Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will bill for their own tech part and make money from that part of the service which they are not currently making revenue from. I would assume that your pathologist are their own group and they don't care who makes the tech part? This is just business and you will have to make your services and prices competitive enough to make it not profitable for the urology group. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, January 07, 2009 10:11 AM To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From rosenfeldtek <@t> hotmail.com Wed Jan 7 13:30:59 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Wed Jan 7 13:31:03 2009 Subject: [Histonet] Microtome Cutting Safety In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70402AD20@LTA3VS011.ees.hhs.gov> References: <11A41625-663C-4843-97D5-B7F480CF4A7D@cox.net> <8666573D-4D9E-41E7-9DF5-8A5ED88171BF@cox.net> <1CE1847DFEA0A647B1CCDE4108EA60A70402AD20@LTA3VS011.ees.hhs.gov> Message-ID: "I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this..." Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 7 Jan 2009 07:25:32 -0500 > From: jqb7@cdc.gov > To: eridana@cox.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Microtome Cutting Safety > CC: > > I use my fingers too, so no help there. > > But an automated microtome is great because it frees both hands to > handle a ribbon as it is coming off the blade. It is easy to get > careless if you use a foot pedal but the one time I have been seriously > cut it was with a manual microtome so I guess the moral is to simply be > careful. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna > Harclerode > Sent: Tuesday, January 06, 2009 11:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome Cutting Safety > > > > Begin forwarded message: > > > From: Donna Harclerode > > Date: January 6, 2009 8:27:14 PM PST > > To: histonet-request@lists.utsouthwestern.edu > > Subject: Microtome Cutting Safety > > > > Anyone know of a paraffin microtome that you can section WITH the > > knife guard in position? I have always used forceps to keep my fingers > > > safe, but some techs are harder to convince to do this . I figured if > > anyone knows they would be on this list. > > I tried the Leica RM2255 (both in automated and manual mode) and I > > could sort of section a couple small blocks with the guard up, but it > > was not going to work with anything except perfect processed small > > blocks and not well for those. > > > > Any opinions on the safety automated versus manual microtomes? I > > adore automated cryostats (Leica 3050 is my favorite) , but I can not > > > figure why use an automated paraffin microtome. Logically I always > > figured an automated can do more damage, but really have no facts. > > > > Thanks in advance, > > > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows LiveTM: Keep your life in sync. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t1_allup_howitworks_012009 From eca9 <@t> georgetown.edu Wed Jan 7 15:46:23 2009 From: eca9 <@t> georgetown.edu (eca9@georgetown.edu) Date: Wed Jan 7 15:47:20 2009 Subject: [Histonet] equation problem PLEASE help Message-ID: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva From beth.millerman <@t> stiefel.com Wed Jan 7 15:53:53 2009 From: beth.millerman <@t> stiefel.com (Beth Millerman) Date: Wed Jan 7 15:55:14 2009 Subject: [Histonet] Microtome Cutting Safety In-Reply-To: Message-ID: I use a fine tipped camel hair brush (sometimes wetted water) to ease the ribbon from the blade. It works great without any danger to the operator. The most that can happen is losing the end of the brush and ending up with a stick. It also prevents damaging the blade holder. On convincing your techs...it could help them develop fine motor movement/cordination if they truely want to develop their professional expertise. Beth Millerman, HT, SRA/SWC Stiefel Laboratories, Inc JR R Sent by: histonet-bounces@lists.utsouthwestern.edu Wed 07 Jan 2009 11:30 AM ------------------------------------------------------- To cc Subject [Histonet ] Microtome Cutting Safety ------------------------------------------------------- "I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this..." Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 7 Jan 2009 07:25:32 -0500 > From: jqb7@cdc.gov > To: eridana@cox.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Microtome Cutting Safety > CC: > > I use my fingers too, so no help there. > > But an automated microtome is great because it frees both hands to > handle a ribbon as it is coming off the blade. It is easy to get > careless if you use a foot pedal but the one time I have been seriously > cut it was with a manual microtome so I guess the moral is to simply be > careful. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna > Harclerode > Sent: Tuesday, January 06, 2009 11:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome Cutting Safety > > > > Begin forwarded message: > > > From: Donna Harclerode > > Date: January 6, 2009 8:27:14 PM PST > > To: histonet-request@lists.utsouthwestern.edu > > Subject: Microtome Cutting Safety > > > > Anyone know of a paraffin microtome that you can section WITH the > > knife guard in position? I have always used forceps to keep my fingers > > > safe, but some techs are harder to convince to do this . I figured if > > anyone knows they would be on this list. > > I tried the Leica RM2255 (both in automated and manual mode) and I > > could sort of section a couple small blocks with the guard up, but it > > was not going to work with anything except perfect processed small > > blocks and not well for those. > > > > Any opinions on the safety automated versus manual microtomes? I > > adore automated cryostats (Leica 3050 is my favorite) , but I can not > > > figure why use an automated paraffin microtome. Logically I always > > figured an automated can do more damage, but really have no facts. > > > > Thanks in advance, > > > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows LiveTM: Keep your life in sync. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t1_allup_howitworks_012009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Wed Jan 7 16:37:12 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Wed Jan 7 16:38:10 2009 Subject: [Histonet] RE: equation problem PLEASE help In-Reply-To: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> References: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> Message-ID: Hi Eva. Here?s how I would do it. (1 mole/1064 grams)*(.001 gram/ml)= 9.4 X 10^-7 mole/ml Concentration 1*Volume 1= Concentration 2 * Volume 2, or C1V1=C2V2 C1= 9.4 X 10^-7 mole/ml C2= 10X 10^-9 mole/ml V2= 2 ml Solving for V1... V1= (C2V2)/C1 V1= (10 X 10^-9 mole/ml*2.0 ml)/ 9.4 X 10^-7 mole/ml=.021 ml You need about 21 microliters of stock solution to make 2 mls of working solution. Use a calculator or Excel to get as many decimal places as you need. Jerry Ricks Research Scientist University of Washington Department of Pathology > From: eca9@georgetown.edu > To: histonet@lists.utsouthwestern.edu > Date: Wed, 7 Jan 2009 16:46:23 -0500 > Subject: [Histonet] equation problem PLEASE help > > Good afternoon, > I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. > The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... > The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. > Thank you, > Eva > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows LiveTM Hotmail?: Chat. Store. Share. Do more with mail. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_hm_justgotbetter_explore_012009 From anh2006 <@t> med.cornell.edu Wed Jan 7 17:40:24 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Jan 7 17:41:27 2009 Subject: [Histonet] Cryostat safety question In-Reply-To: References: Message-ID: The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- From Jan.Minshew <@t> leica-microsystems.com Wed Jan 7 18:17:38 2009 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Wed Jan 7 18:17:31 2009 Subject: [Histonet] Cryostat safety question In-Reply-To: Message-ID: Hi Andrea, It's great to hear that you are happy with your cryostat and, hopefully, I'll be able to help you with an answer to your question. Leica sells a small device called a Thermal Block that is designed to help you remove the specimen from the chuck without causing it to melt. The catalog number is 14039818542. It's a small, mobile device that can be used in any of our cryostats that use chucks with stems in the back (we have a different one for the new CM1950 flat backed chucks). If you have questions or would like additional information, please feel free to contact our Technical Applications Center (1-800-248-0123 option 1 then option 2). Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! "Andrea Hooper" To Sent by: Histonet histonet-bounces@ lists.utsouthwest cc ern.edu mari.ann.mailhiot@leica-microsystem s.com Subject 01/07/2009 05:40 [Histonet] Cryostat safety question PM The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From CIngles <@t> uwhealth.org Wed Jan 7 18:38:04 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jan 7 18:39:06 2009 Subject: [Histonet] Cryostat safety question References: Message-ID: Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop or so to the already frozen 'button' and immediately embed the tissue in it. We usually add another small drop on top after it has begun to freeze, to cover the specimen completely. Cut as normal when frozen. After done cutting all you have to do is use a forceps or other blunt object and pop the bit with the specimen in it away from the 'button' and return the chuck to the cryostat and it can be reused the rest of the day. The specimen is therefore still frozen for storage, and it has a quicker TAT. Plus you won't need nearly so many chucks, as they can be recycled almost as soon as you are done cutting. I usually keep 6-8 'buttons' in my cryostat, and our clinic can process up to 50 separate specimens a day. A word of caution. If your work area is humid sometimes a thin layer of frost can form on the surface of the 'button' and when you attempt to take sections the bit with the tissue will pop off the 'button'. All you need to do is add another drop of medium to the button and 'glue' the two back together. If you are going a while between cutting sessions, I usually store my 'buttons' upside(mountant side) down on one of the cryostat surfaces. It doesn't seem to develop the frost layer. Useful if you have tiny specimens. Hope my verbose explanation is helpful. Feel free to e-mail if you have any questions or are confused about my explanation. Claire Ingles Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Wed 1/7/2009 5:40 PM To: Histonet Cc: mari.ann.mailhiot@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beth.millerman <@t> stiefel.com Wed Jan 7 18:48:27 2009 From: beth.millerman <@t> stiefel.com (Beth Millerman) Date: Wed Jan 7 18:49:29 2009 Subject: [Histonet] Cryostat safety question In-Reply-To: Message-ID: I have the same cyostat, but it doesn't matter which one you use for removing tissue from the chuck is a separate issue. Others may beg to differ from my technic, but I remove the block from the cryostat and set it on a paper towel for a couple of mintes. I do not thaw tissue, just soften it. I take a scapel or small weigh spatula and gently separate it off the chuck. With a thin layer of OCT in the plastic mold, I return the block back to its original labeled mold, wrap with aluminum foil, place in a ziploc, and store it back to the -80C. This is quick and limits exposure to higher temps. I have used blocks multiple times, especially for cross reactivity studies and never had a problem. I am very careful and need reusing the block for IHC receptor studies. Good luck. This is pretty easy and you can move on to the next block at the same time. Beth Millerman/SWC Senior Research Associate Stiefel Laboratories, Inc "Andrea Hooper" Sent by: histonet-bounces@lists.utsouthwestern.edu Wed 07 Jan 2009 03:40 PM ------------------------------------------------------- To Histonet cc mari.ann. mailhiot@ leica-mic rosystems .com Subject [Histonet ] Cryostat safety question ------------------------------------------------------- The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Wed Jan 7 19:03:13 2009 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Jan 7 19:03:24 2009 Subject: R: [Histonet] equation problem PLEASE help In-Reply-To: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> Message-ID: <139631.17022.qm@web23305.mail.ird.yahoo.com> Your final solution: 1ml. will contain 10^-8 mole Its molarit? will be: M = (10^-8 * 10^3)/1 = 10^-5 (mole / litre) Starting solution: 1ml. contains 10^-3/1064 = 9.40*10^-7 mole So you have to take: 1 : (9.40*10^-7) = Xml : 10^-5 Xml = (10^-5) / (9.40*10^-7) = 10,64 ml. of starting solution and bring them to 1 litre by adding 989.36 ml. of distilled water. Best Regards, Massimo Tosi --- Mer 7/1/09, eca9@georgetown.edu ha scritto: Da: eca9@georgetown.edu Oggetto: [Histonet] equation problem PLEASE help A: histonet@lists.utsouthwestern.edu Data: Mercoled? 7 gennaio 2009, 22:46 Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jan 7 20:24:52 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jan 7 20:28:32 2009 Subject: [Histonet] Microtome Cutting Safety References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF766@LTA3VS011.ees.hhs.gov> I guess I wasn't totally clear in my earlier reply. I use my bare fingers but I also use a camel hair brush as Beth states. I direct the brush with my right hand and pick up the first part of the ribbon with my left. The brush is used to hold the last part of the ribbon to aid in maneuvering to the waterbath. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Beth Millerman Sent: Wed 1/7/2009 4:53 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Cutting Safety I use a fine tipped camel hair brush (sometimes wetted water) to ease the ribbon from the blade. It works great without any danger to the operator. The most that can happen is losing the end of the brush and ending up with a stick. It also prevents damaging the blade holder. On convincing your techs...it could help them develop fine motor movement/cordination if they truely want to develop their professional expertise. Beth Millerman, HT, SRA/SWC Stiefel Laboratories, Inc JR R Sent by: histonet-bounces@lists.utsouthwestern.edu Wed 07 Jan 2009 11:30 AM ------------------------------------------------------- To cc Subject [Histonet ] Microtome Cutting Safety ------------------------------------------------------- "I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this..." Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 7 Jan 2009 07:25:32 -0500 > From: jqb7@cdc.gov > To: eridana@cox.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Microtome Cutting Safety > CC: > > I use my fingers too, so no help there. > > But an automated microtome is great because it frees both hands to > handle a ribbon as it is coming off the blade. It is easy to get > careless if you use a foot pedal but the one time I have been seriously > cut it was with a manual microtome so I guess the moral is to simply be > careful. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna > Harclerode > Sent: Tuesday, January 06, 2009 11:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome Cutting Safety > > > > Begin forwarded message: > > > From: Donna Harclerode > > Date: January 6, 2009 8:27:14 PM PST > > To: histonet-request@lists.utsouthwestern.edu > > Subject: Microtome Cutting Safety > > > > Anyone know of a paraffin microtome that you can section WITH the > > knife guard in position? I have always used forceps to keep my fingers > > > safe, but some techs are harder to convince to do this . I figured if > > anyone knows they would be on this list. > > I tried the Leica RM2255 (both in automated and manual mode) and I > > could sort of section a couple small blocks with the guard up, but it > > was not going to work with anything except perfect processed small > > blocks and not well for those. > > > > Any opinions on the safety automated versus manual microtomes? I > > adore automated cryostats (Leica 3050 is my favorite) , but I can not > > > figure why use an automated paraffin microtome. Logically I always > > figured an automated can do more damage, but really have no facts. > > > > Thanks in advance, > > > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows LiveTM: Keep your life in sync. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t1_allup_howitworks_012009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Wed Jan 7 20:39:19 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed Jan 7 20:40:15 2009 Subject: [Histonet] equation problem PLEASE help In-Reply-To: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> References: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> Message-ID: <4965F3F6.471C.0039.0@health.qld.gov.au> Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.00001064g or 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0 = 94 (93.984962 exactly) 0.01064 Therefore you can add 1ml stock to 93ml of diluent to get your desired concentration.. I hope this is useful regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> 8/01/2009 7:46 am >>> Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Tony_Reilly <@t> health.qld.gov.au Wed Jan 7 21:13:57 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed Jan 7 21:15:52 2009 Subject: [Histonet] equation problem PLEASE help In-Reply-To: <4965F3F6.471C.0039.0@health.qld.gov.au> References: <20090107164623.ABS52453@mstore-prod-2.pdc.uis.georgetown.edu> <4965F3F6.471C.0039.0@health.qld.gov.au> Message-ID: <4965FC15.471C.0039.0@health.qld.gov.au> Hello Eva Unfortunately some of the script did not transfer in the original mail and 10-9 should read 10 to the minus 9 as the superscript was lost. The same for 10-8. Also there should have been a division line between 1.0 and 0.01064 in the calculation. I hope this helps. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Anthony Reilly" 8/01/2009 12:39 pm >>> Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.00001064g or 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0 = 94 (93.984962 exactly) 0.01064 Therefore you can add 1ml stock to 93ml of diluent to get your desired concentration.. I hope this is useful regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> 8/01/2009 7:46 am >>> Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Thu Jan 8 02:39:37 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Jan 8 02:39:55 2009 Subject: [Histonet] RE: double immunoenzymatic methods Message-ID: <8190197e1d540ebf.4965c9d9@amc.uva.nl> Hi Kim,The best shot I can offer you is trying the method we published in JOH last September. PDF is attached with mail to your work address. That method is based on two AP staining methods with an additional HIER step in between. The second HIER step destroys/elutes the antibodies used in the first staining sequence. That prevents unwanted cross-reactions with the second staining sequence. The red-blue color combination allows to observe co-localization in a purple-blue mixed-color. Main drawback is the blue reaction product: it's a bit fuzzy compared to DAB reaction product. Take care to adapt the dilutions of your primaries, because the staining efficiency of the red and blue reaction products might be different from DAB in black. Good luck!Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 07 Jan 2009 12:12:05 +1100 From: Kim O'Sullivan Subject: [Histonet] double immunoenzymatic methods To: histonet@lists.utsouthwestern.edu Hi, Have been having trouble getting an antibody to work for double immunofluoresecence (with the confocal microscope), but am able to get it to work on FFPE tissue with DAB black. Does anyone out there have a method I can use that will show colocalisation using enzyme labelling methods, ie which colour choices do you make to show double labelling within the same structure I understand this is not the preferred method. Regards Kim O'Sullivan From c.m.vanderloos <@t> amc.uva.nl Thu Jan 8 03:03:52 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Jan 8 03:04:08 2009 Subject: [Histonet] RE: IHC Doublestain Message-ID: Kim,This is a difficult one! First HIER and then prot K may destroy/remove antigens, not to speak about the integrity of the tissue morphology! The best you can do is first performing HIER and then prot K only half of the time. Again, it fully depends on the antigens of interest if both will be still there after this harsh treatment. If you are using a DAKO sequential double staining method you may start with HIER followed by the 1st primary antibody (the one that needs HIER), detection system, finish with DAB in brown, then prot K (half of the time) followed by the 2nd primary, detection sytem, AP in red. Alternatively, you can follow the same sequential procedure with two AP methods as we described in JOH of last September. PDF is send to your work address. The second best I can offer you is to find a HIER method that works for both antigens. HIER with Tris/EDTA pH9.0 is effective for many antigens that are usually enzymatically pretreated. Good luck!ChrisChris van der Lo! os, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 07 Jan 2009 11:21:13 -0500 From: "Kimberly Tuttle" Subject: [Histonet] IHC Doublestain To: histonet@lists.utsouthwestern.edu I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 From pieronelva01 <@t> bigpond.com Thu Jan 8 04:33:13 2009 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Thu Jan 8 04:33:26 2009 Subject: [Histonet] RE: IHC Doublestain References: Message-ID: <57B7A116A61F4965B1E7D6462D0EA69A@pentium4> Hi Kimberly I think that this is a classic "try it and see" problem :-), but HEIR before enzyme sounds best. I've combined dual staining, but only with the same pretreatment. It may be a case of compromising on the staining intensity for each antibody, rather thatn optimising either. THat said, I always like to see dual staining. Sometimes there is just too much DAB brown and a splash of any other colour brightens my IHC day. Regards Piero Nelva Anatomical PAthology Monash Medical Centre ----- Original Message ----- From: "C.M. van der Loos" To: Sent: Thursday, January 08, 2009 8:03 PM Subject: [Histonet] RE: IHC Doublestain > Kim,This is a difficult one! First HIER and then prot K may destroy/remove > antigens, not to speak about the integrity of the tissue morphology! The > best you can do is first performing HIER and then prot K only half of the > time. Again, it fully depends on the antigens of interest if both will be > still there after this harsh treatment. If you are using a DAKO sequential > double staining method you may start with HIER followed by the 1st primary > antibody (the one that needs HIER), detection system, finish with DAB in > brown, then prot K (half of the time) followed by the 2nd primary, > detection sytem, AP in red. Alternatively, you can follow the same > sequential procedure with two AP methods as we described in JOH of last > September. PDF is send to your work address. The second best I can offer > you is to find a HIER method that works for both antigens. HIER with > Tris/EDTA pH9.0 is effective for many antigens that are usually > enzymatically pretreated. Good luck!ChrisChris van der Lo! > os, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands Date: Wed, 07 Jan 2009 11:21:13 -0500 > From: "Kimberly Tuttle" > Subject: [Histonet] IHC Doublestain > To: histonet@lists.utsouthwestern.edu > > I am supposed to try a doublestain using the DAKO autostainer where one > of the antibodies requires a Proteinase K pretreatment and the other > antibody requires antigen retrieval with heat. Should I do both, or will > this not work at all? Thank you > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.176 / Virus Database: 270.10.5/1881 - Release Date: 1/7/2009 5:59 PM From drvet_anjan <@t> hotmail.com Thu Jan 8 05:35:52 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Thu Jan 8 05:36:52 2009 Subject: [Histonet] chromogens for triple immunohistochemistry Message-ID: hi, I am very thankful to everyone for their replies to my questions. i had another one which is quite confusing and it is like i wanted to know which three colour chromogen combinations is best for triple immunohistochemistry. As all antigen of my interest are all co localized. regards, anjan kumar, Junior research scientist, Madras veterinary college. _________________________________________________________________ Drag n? drop?Get easy photo sharing with Windows Live? Photos. http://www.microsoft.com/windows/windowslive/photos.aspx From abright <@t> brightinstruments.com Thu Jan 8 06:06:26 2009 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Jan 8 06:06:34 2009 Subject: [Histonet] Cryostat safety question In-Reply-To: References: Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0ACD8A@BRIGHT-SBS.Bright.local> Andrea, For users of our cryostats that need to use the specimen for future use we manufacture disposable object holders & quick release holders that fits specimen containers for LN2 or -80 deg. C storage. The tissue stays on the disposable object holders during storage too and has an index location so that it only goes back onto the cryostat microtome in the correct orientation. No sorry but it will not be suitable for your cryostat or other Chinese/ German manufactured types. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: 07 January 2009 23:40 To: Histonet Cc: mari.ann.mailhiot@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Jan 8 06:11:26 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Jan 8 06:11:35 2009 Subject: [Histonet] Dental Histology. Message-ID: <1D3332273DCF4C51BFC68B71E178A89B@IBLS.GLA.AC.UK> I now find myself, after many years away, coming back to dental histology so need a bit of advice on current techniques. Ground sections, I still have the technique. Fully mineralized, embed in resin and section using a tungsten carbide knife. Deminerailized, here I'm not sure. I'm going back 30-40 years when I used formic acid but is there anything better that is recommended? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From jshelley <@t> burnham.org Thu Jan 8 06:41:51 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Thu Jan 8 06:41:58 2009 Subject: [Histonet] Poly Scientific's Oil Red O with Propylene Glycol Message-ID: Hello Histonetters, For those of you who are using this kit(k043) from Poly Scientific for Oil Red O. I'm staining heart tissue that was fixed in 4%PFA but when I look at the slides they don't appear to have any fat and I know some of them should. Any special tips on using this kit that you have found to work. For all who want to tell me of other methods that may work better, that maybe a request for any other time. Thanks in advance for any pearls of wisdom. John J Shelley Histology Core Facility Burnham Institute for Medical Research From jshelley <@t> burnham.org Thu Jan 8 07:19:16 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Thu Jan 8 07:19:24 2009 Subject: [Histonet] Poly Scientific's Oil Red O with Propylene Glycol-Clarification Message-ID: This is using frozen sections that were previously cut a few days ago and placed in a -20 C freezer ________________________________ From: John Shelley Sent: Thursday, January 08, 2009 7:42 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Poly Scientific's Oil Red O with Propylene Glycol Hello Histonetters, For those of you who are using this kit(k043) from Poly Scientific for Oil Red O. I'm staining heart tissue that was fixed in 4%PFA but when I look at the slides they don't appear to have any fat and I know some of them should. Any special tips on using this kit that you have found to work. For all who want to tell me of other methods that may work better, that maybe a request for any other time. Thanks in advance for any pearls of wisdom. John J Shelley Histology Core Facility Burnham Institute for Medical Research From rjbuesa <@t> yahoo.com Thu Jan 8 08:23:49 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 8 08:24:48 2009 Subject: [Histonet] Dental Histology. In-Reply-To: <1D3332273DCF4C51BFC68B71E178A89B@IBLS.GLA.AC.UK> Message-ID: <268750.48012.qm@web65715.mail.ac4.yahoo.com> Hi Ian: The Boedecker's fluid (1937) is considered as very good for enamel. It consists of methanol (100 mL) + celloidin (6 mL) + nitric acid (4.5 mL) and it is reported to work well. Regards Ren? J. --- On Thu, 1/8/09, Ian Montgomery wrote: From: Ian Montgomery Subject: [Histonet] Dental Histology. To: histonet@lists.utsouthwestern.edu Date: Thursday, January 8, 2009, 7:11 AM I now find myself, after many years away, coming back to dental histology so need a bit of advice on current techniques. Ground sections, I still have the technique. Fully mineralized, embed in resin and section using a tungsten carbide knife. Deminerailized, here I'm not sure. I'm going back 30-40 years when I used formic acid but is there anything better that is recommended? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Jan 8 09:26:02 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jan 8 09:48:00 2009 Subject: [Histonet] RE: IHC Doublestain In-Reply-To: References: Message-ID: <1833208877-1231429671-cardhu_decombobulator_blackberry.rim.net-933665427-@bxe294.bisx.prod.on.blackberry> Great advice. Unless you developed the retrieval protocols yourself, I also suggest you try running your stains with the other method of antigen retrival. Just test it and see what happens. You don't know how many times I am told something only works with a certain method to find out it actually works with many retrieval methods. You might just be able to do one of the methods for both antigens, which would make your life a lot easier. -----Original Message----- From: "C.M. van der Loos" Date: Thu, 08 Jan 2009 10:03:52 To: Subject: [Histonet] RE: IHC Doublestain Kim,This is a difficult one! First HIER and then prot K may destroy/remove antigens, not to speak about the integrity of the tissue morphology! The best you can do is first performing HIER and then prot K only half of the time. Again, it fully depends on the antigens of interest if both will be still there after this harsh treatment. If you are using a DAKO sequential double staining method you may start with HIER followed by the 1st primary antibody (the one that needs HIER), detection system, finish with DAB in brown, then prot K (half of the time) followed by the 2nd primary, detection sytem, AP in red. Alternatively, you can follow the same sequential procedure with two AP methods as we described in JOH of last September. PDF is send to your work address. The second best I can offer you is to find a HIER method that works for both antigens. HIER with Tris/EDTA pH9.0 is effective for many antigens that are usually enzymatically pretreated. Good luck!ChrisChris van der Lo! os, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 07 Jan 2009 11:21:13 -0500 From: "Kimberly Tuttle" Subject: [Histonet] IHC Doublestain To: histonet@lists.utsouthwestern.edu I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Jan 8 09:43:57 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jan 8 09:49:15 2009 Subject: [Histonet] Cryostat safety question In-Reply-To: References: Message-ID: <1286432121-1231429745-cardhu_decombobulator_blackberry.rim.net-776419083-@bxe294.bisx.prod.on.blackberry> Very ingenious technique Claire! However, I would hesitate to use this with our particular cryostat as I find when doing this type of technique (keeping a button), or using any chucks which are not absolutely RT I get thick-thin sections as the tissue isn't super firmly adhered to the chucks. If I use super clean, room temp chucks my sections are 100% better quality. In fact when teaching users to use the cryostat I always make a big deal about this issue. The thick-thin issue seems to be improved somewhat since Leica revamped their chucks and now have much bigger grooves - so I am convinced firm adherance is key to quality sections. I would be curious if anyone had any opinions on this, or experienced the same thing (thick thin sections as a result of chuck/tissue adherance). If Leica peops want to chime in about it too, I would welcome that. Andrea -----Original Message----- From: Ingles Claire Date: Wed, 07 Jan 2009 18:38:04 To: Histonet Cc: Subject: RE: [Histonet] Cryostat safety question Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop or so to the already frozen 'button' and immediately embed the tissue in it. We usually add another small drop on top after it has begun to freeze, to cover the specimen completely. Cut as normal when frozen. After done cutting all you have to do is use a forceps or other blunt object and pop the bit with the specimen in it away from the 'button' and return the chuck to the cryostat and it can be reused the rest of the day. The specimen is therefore still frozen for storage, and it has a quicker TAT. Plus you won't need nearly so many chucks, as they can be recycled almost as soon as you are done cutting. I usually keep 6-8 'buttons' in my cryostat, and our clinic can process up to 50 separate specimens a day. A word of caution. If your work area is humid sometimes a thin layer of frost can form on the surface of the 'button' and when you attempt to take sections the bit with the tissue will pop off the 'button'. All you need to do is add another drop of medium to the button and 'glue' the two back together. If you are going a while between cutting sessions, I usually store my 'buttons' upside(mountant side) down on one of the cryostat surfaces. It doesn't seem to develop the frost layer. Useful if you have tiny specimens. Hope my verbose explanation is helpful. Feel free to e-mail if you have any questions or are confused about my explanation. Claire Ingles Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Wed 1/7/2009 5:40 PM To: Histonet Cc: mari.ann.mailhiot@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Jan 8 09:29:39 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jan 8 09:49:20 2009 Subject: [Histonet] Cryostat safety question Message-ID: <2003653539-1231429696-cardhu_decombobulator_blackberry.rim.net-2007681632-@bxe294.bisx.prod.on.blackberry> That sounds very cool for the lucky owners of one of your cryostats, esp the part about orientation ... but nearly every block of ours goes back in the freezer so I think it would take up too much space and end up being expensive, no (we are talking 100s and 100s of block)? ------Original Message------ From: Alan Bright To: Andrea Hooper To: Histonet Sent: Jan 8, 2009 7:06 AM Subject: RE: [Histonet] Cryostat safety question Andrea, For users of our cryostats that need to use the specimen for future use we manufacture disposable object holders & quick release holders that fits specimen containers for LN2 or -80 deg. C storage. The tissue stays on the disposable object holders during storage too and has an index location so that it only goes back onto the cryostat microtome in the correct orientation. No sorry but it will not be suitable for your cryostat or other Chinese/ German manufactured types. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: 07 January 2009 23:40 To: Histonet Cc: mari.ann.mailhiot@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfountain <@t> exchange.hsc.mb.ca Thu Jan 8 10:46:37 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Thu Jan 8 10:46:41 2009 Subject: [Histonet] Looking for CD41 for FFPE Message-ID: I am looking for a CD41/GpIIb antibody for formalin fixed paraffin embedded tissue (prefer clone 5B12) but I can't seem to find any manufacturers. Any ideas? Thanks, Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From arsenn <@t> hsh.org Thu Jan 8 11:02:30 2009 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Thu Jan 8 11:02:35 2009 Subject: [Histonet] Outside lab Paula Message-ID: YEP, Paula's right............... From: Paula Pierce Subject: Re: [Histonet] Outside Lab To: Mike Pence , Histonet Message-ID: <915918.43282.qm@web1104.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The pathologists are probably going to the urology group to read because CLIA certification is LOCATION specific. The urology group may already have CLIA cert. and is splitting the interpretation charges with the pathologist. By adding its own lab, they will be able to capture both tech and interp charges. Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From kdboydhisto <@t> yahoo.com Thu Jan 8 11:17:05 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Jan 8 11:17:09 2009 Subject: [Histonet] Looking for CD41 for FFPE Message-ID: <595033.49305.qm@web58606.mail.re3.yahoo.com> Try this web site.? Biocompare.com. You put in the antibody you are looking for and it will show all the manufactures that make it. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 1/8/09, Tiana Fountain wrote: From: Tiana Fountain Subject: [Histonet] Looking for CD41 for FFPE To: histonet@lists.utsouthwestern.edu Date: Thursday, January 8, 2009, 11:46 AM I am looking for a CD41/GpIIb antibody for formalin fixed paraffin embedded tissue (prefer clone 5B12) but I can't seem to find any manufacturers. Any ideas? Thanks, Tiana -----Inline Attachment Follows----- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information.? Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.? If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From S.J.Ainsworth <@t> bsms.ac.uk Thu Jan 8 11:17:41 2009 From: S.J.Ainsworth <@t> bsms.ac.uk (S.J.Ainsworth@bsms.ac.uk) Date: Thu Jan 8 11:19:24 2009 Subject: [Histonet] LacZ and cryosectioning Message-ID: <397EBB11B9C649428A1AB64BC16C66AA01911339@EXCHANGE2.university.brighton.ac.uk> Hi, I?ve been having some trouble x-gal staining some cryosections of chick hindlimb tissue. I?ve been cutting 15 ?m sections then x-gal staining (using a protocol from another lab that should work) and counter staining with nuclear fast red. The sections look good with the nuclear fast red, but the x-gal staining only shows up as very faint speckling at x40. I routinely x-gal stain whole chick embryos then wax section and the blue of the x-gal stain is always very bright (both on the embryo before any tissue is removed for sectioning and in the wax sections). To try and find out if the problem was occurring before or after sectioning I defrosted some limbs (that had not as yet been cryosectioned) and x-gal stained them using the method that I use routinely, they showed none of the blue staining I would expect. Has anyone heard of snap freezing knocking down the activity of the ?-galactosidase enzyme or is able to suggest anything I could try to solve this, please? Thanks Sophie Sophie Ainsworth Brighton and Sussex Medical school University of Sussex Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 From Mary.L.Ring <@t> HealthPartners.Com Thu Jan 8 09:50:00 2009 From: Mary.L.Ring <@t> HealthPartners.Com (Ring, Mary L) Date: Thu Jan 8 11:30:43 2009 Subject: [Histonet] Hematoxylin Counterstain for IHC slides Message-ID: <72A7842F855ACB4D8DB4BDD232E216E6043106F2@hpes3.HealthPartners.int> Hello! I am looking for a good hematoxylin counterstain for IHC slides. My pathologists are familiar with the bright blue color of Ventana's hematoxylin counterstain, so I am looking for something similar. Thanks for your help! Mary ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From anh2006 <@t> med.cornell.edu Thu Jan 8 12:05:48 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jan 8 12:08:23 2009 Subject: [Histonet] Hematoxylin Counterstain for IHC slides In-Reply-To: <72A7842F855ACB4D8DB4BDD232E216E6043106F2@hpes3.HealthPartners.int> References: <72A7842F855ACB4D8DB4BDD232E216E6043106F2@hpes3.HealthPartners.int> Message-ID: <706555941-1231438093-cardhu_decombobulator_blackberry.rim.net-96699285-@bxe294.bisx.prod.on.blackberry> I use Dako Mayer's for 5 seconds followed by roughly a minute in bueing solution. Gorgeous blue. -----Original Message----- From: "Ring, Mary L" Date: Thu, 08 Jan 2009 09:50:00 To: Subject: [Histonet] Hematoxylin Counterstain for IHC slides Hello! I am looking for a good hematoxylin counterstain for IHC slides. My pathologists are familiar with the bright blue color of Ventana's hematoxylin counterstain, so I am looking for something similar. Thanks for your help! Mary ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Jan 8 12:33:57 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jan 8 12:36:32 2009 Subject: [Histonet] LacZ and cryosectioning In-Reply-To: <397EBB11B9C649428A1AB64BC16C66AA01911339@EXCHANGE2.university.brighton.ac.uk> References: <397EBB11B9C649428A1AB64BC16C66AA01911339@EXCHANGE2.university.brighton.ac.uk> Message-ID: <1505728483-1231439782-cardhu_decombobulator_blackberry.rim.net-295456701-@bxe294.bisx.prod.on.blackberry> We do X-gal on snap frozen tissue all the time, so I can try to help. Can you give more specifics about your protocol? Your tissue is fresh frozen, correct? In OCT? Specifically what fixative are you using on your sections and for how long? Are you using Ca2+ and Mg2+ free PBS? Are you positive your tissue should be a strong stainer - if not do you have a sure-fire positive control? Andrea -----Original Message----- From: S.J.Ainsworth@bsms.ac.uk Date: Thu, 08 Jan 2009 17:17:41 To: Subject: [Histonet] LacZ and cryosectioning Hi, I?ve been having some trouble x-gal staining some cryosections of chick hindlimb tissue. I?ve been cutting 15 ?m sections then x-gal staining (using a protocol from another lab that should work) and counter staining with nuclear fast red. The sections look good with the nuclear fast red, but the x-gal staining only shows up as very faint speckling at x40. I routinely x-gal stain whole chick embryos then wax section and the blue of the x-gal stain is always very bright (both on the embryo before any tissue is removed for sectioning and in the wax sections). To try and find out if the problem was occurring before or after sectioning I defrosted some limbs (that had not as yet been cryosectioned) and x-gal stained them using the method that I use routinely, they showed none of the blue staining I would expect. Has anyone heard of snap freezing knocking down the activity of the ?-galactosidase enzyme or is able to suggest anything I could try to solve this, please? Thanks Sophie Sophie Ainsworth Brighton and Sussex Medical school University of Sussex Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 From kdboydhisto <@t> yahoo.com Thu Jan 8 13:46:51 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Jan 8 13:46:55 2009 Subject: [Histonet] Hematoxylin Counterstain for IHC slides Message-ID: <835298.41532.qm@web58604.mail.re3.yahoo.com> Biocare's Cat Hematoxylin is really good. One real nice feature is that you blue your slides in TBS. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 1/8/09, anh2006@med.cornell.edu wrote: From: anh2006@med.cornell.edu Subject: Re: [Histonet] Hematoxylin Counterstain for IHC slides To: "Ring, Mary L" , histonet@lists.utsouthwestern.edu Date: Thursday, January 8, 2009, 1:05 PM I use Dako Mayer's for 5 seconds followed by roughly a minute in bueing solution. Gorgeous blue. -----Original Message----- From: "Ring, Mary L" Date: Thu, 08 Jan 2009 09:50:00 To: Subject: [Histonet] Hematoxylin Counterstain for IHC slides Hello! I am looking for a good hematoxylin counterstain for IHC slides. My pathologists are familiar with the bright blue color of Ventana's hematoxylin counterstain, so I am looking for something similar. Thanks for your help! Mary ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jan 8 15:02:15 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Jan 8 15:02:29 2009 Subject: [Histonet] Re: Removing frozen tissue blocks from metal disks after cryotomy Message-ID: <000c01c971d4$62a00730$27e01590$@callis@bresnan.net> Andrea, We seal a cryosectioned block with a very thin layer of OCT first. How to's follow: Use a blade guard, lock flywheel and put a drop of OCT on your thumb, rub quickly across block face and make sure tissue is "smeared over" with a thin layer of OCT. We want this layer to freeze as quickly as possible. We do this while the disk is still in the microtome holder, and can then mark (with a sharpie marker) the block if you need to identify. Don't put a huge blob of OCT on the face as this warms the tissue too much until it freezes again. Take the disk out of holder, and warm the back of disk with hand. Our disks have stems so placing the stems between the fingers warms the metal allows warming the back efficiently, then the OCT warms but does not melt the block. The block should pop off the disk by using a thin, metal spatula, dull but thin blade paring knife, or a mini-cheese spreader recycled from a kitchen - the small ones used at parties for spreading cheeses, etc or a single edge razor blade inserted NEXT to the disk, between disk and OCT, then twisting. We use single edge razor blades(teflon coated) at RT, so this works even better. It is better to use a thin, dull metal edge for this instead of razor blades to prevent bad cuts to your fingers. We have even lined up sealed blocks in a plastic micro-centrifuge tube block holder - at room temperature, and let the OCT start to warm up for block removal. This requires careful attention so the blocks do not start to melt, so work quickly. Some people are sensitive to cold, and this is another way to do this or keep changing the block around to be between different fingers. Whatever you do, DO NOT hold the disk up and out of chamber and then try to cut the block OFF the disk. Do NOT try to cut through the OCT if the back of block has not been warmed. I have had people cut themselves with razor blades trying to do this. If you brace the disk in the little brush holding tray in front of knife holder , you can steady the disk/block, and pop the block off without cutting yourself. Sort of like cutting a slab of cheese or salami. After the block is removed, we trim excess OCT from block where the media has spread out during freezing onto the disk, in other words, reshape the block back to a square or rectangle. This is so the block fits nicely into storage tube. Wrap the whole block with aluminum foil, and slip the blocks into 50 ml centrifuge tubes. The foil can be marked with sharpie marker to identify the tissue. We keep aluminum foil squares on hand for this purpose. We keep cryosectioned tissue blocks for years, and have had total success doing murine CD markers even after 7 years of storage. The key is to reseal the block, and store at -80C. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT From hfedor <@t> jhmi.edu Thu Jan 8 15:14:18 2009 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Jan 8 15:15:16 2009 Subject: [Histonet] Cryostat safety question In-Reply-To: References: Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D3168D02CA15@JHEMTEXVS3.win.ad.jhu.edu> Hello, I had previously sent this but only to the person who asked the question, I think it this works beautifully so decided to resend to the list. we have a great way to remove the specimens for the chucks. We have a 500cc plastic container with a lid that we have cut an "X" into the center. We put warm water in the container and then just put the chuck stem into the "X". Within 10 seconds the block can be removed. Still frozen. We just keep the plastic container at the bench and keep reusing the same one. The water level does need to come all the way up to the lid, But the chuck never gets wet. We do the sealing of the tissue with a tiny amount of OCT to the surface of the still frozen block, while it is still in the cryostat as well. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, January 07, 2009 7:38 PM To: Histonet Cc: mari.ann.mailhiot@leica-microsystems.com Subject: RE: [Histonet] Cryostat safety question Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop or so to the already frozen 'button' and immediately embed the tissue in it. We usually add another small drop on top after it has begun to freeze, to cover the specimen completely. Cut as normal when frozen. After done cutting all you have to do is use a forceps or other blunt object and pop the bit with the specimen in it away from the 'button' and return the chuck to the cryostat and it can be reused the rest of the day. The specimen is therefore still frozen for storage, and it has a quicker TAT. Plus you won't need nearly so many chucks, as they can be recycled almost as soon as you are done cutting. I usually keep 6-8 'buttons' in my cryostat, and our clinic can process up to 50 separate specimens a day. A word of caution. If your work area is humid sometimes a thin layer of frost can form on the surface of the 'button' and when you attempt to take sections the bit with the tissue will pop off the 'button'. All you need to do is add another drop of medium to the button and 'glue' the two back together. If you are going a while between cutting sessions, I usually store my 'buttons' upside(mountant side) down on one of the cryostat surfaces. It doesn't seem to develop the frost layer. Useful if you have tiny specimens. Hope my verbose explanation is helpful. Feel free to e-mail if you have any questions or are confused about my explanation. Claire Ingles Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Wed 1/7/2009 5:40 PM To: Histonet Cc: mari.ann.mailhiot@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question .... We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From olvluis <@t> hotmail.com Thu Jan 8 15:35:48 2009 From: olvluis <@t> hotmail.com (jose olvera) Date: Thu Jan 8 15:35:52 2009 Subject: [Histonet] List of Main Hospitals in san francisco. Message-ID: Dear Histonets: I would appreciate greatly any information regarding to listing the Main hospitals for cancer in San Francisco, Ca. Thanks in advance. Luis. _________________________________________________________________ Windows Live? Hotmail?: Chat. Store. Share. Do more with mail. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_hm_justgotbetter_explore_012009 From gayle.callis <@t> bresnan.net Thu Jan 8 15:46:35 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Jan 8 15:47:44 2009 Subject: [Histonet] metal chucks or disks that hold blocks firmly for cryotomy In-Reply-To: References: Message-ID: <002001c971da$93d6d8f0$bb848ad0$@callis@bresnan.net> For cryostats that have disks with stems, we learned disks with a waffle weave, grooved mesh top holds blocks far better than disks with the circles of raised metal i.e. these look like a "target." We prefer waffle weave disks for large blocks, vey dense tissue, decalcified and calcified bone cryotomy. Chatter is not a factor when using these blocks, but we also use only high profile disposable blades. We get far more chatter with the disks that look like targets (circles of raised metal) Our Leica Cryocut 1850's came with three target style disks with stems. We had a whole array of sizes (waffle weave) left from years past when waffle weave metal chucks were used for paraffin blocks. For more, we purchased the waffle weave disks from EMS. It was worth the investment plus they come in various sizes. You have to cut the stems shorter so they match the disk stems and fit into the microtome block holder. We often line up 20 or more blocks for a cryotomy session, and not having enough chucks is a pain. Time is money and buying more resuable disks was the best option for efficiency and speed. Some cryostats now come with the no-stem waffle weave disks, and these are excellent. For the boneheads cutting undecalcified bone frozen sections, the waffle weave chucks are superior since they are heavier with the extra deep groove for better holding capability. We use a different media to mount (not for embedding) undecalcified bone on a chuck, as OCT is a bit softer than what we use. 2% methyl cellulose (Aldrich for chemical) sets up like concrete when frozen but is water soluble when melted and you need to remove the goo from the disk. You can also use wall paper paste, a methyl celluose product! Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 From ACortez <@t> hei.org Thu Jan 8 16:38:56 2009 From: ACortez <@t> hei.org (Cortez, Ana) Date: Thu Jan 8 16:39:03 2009 Subject: [Histonet] Laser capture microdissection Message-ID: <87449E4A2B01DA47B29424CE5D6E0F8306F40080@hi0sml1.hei.org> Hi everyone, I would like to know if anyone is in possetion of a Laser Pro 300 and Laser tweezers Instruction Manual. For those who don't know what this is-- it is a Laser capture microdissection machine (an old version), manufactured by Cell Robotics (which does'nt exist anymore). We own the machine, but the instruction manual has disapeared. If someone has the same machine and still has the manual, would you please let me know. I would be most appreciative. Thank you. Ana Cordero, HT(ASCP)CM Research Associate House Ear Institute 2100 W. 3rd Street Los Angeles, CA 90057 213-353-7088 acortez@hei.org From tatiana.gonzalez <@t> leo-pharma.com Fri Jan 9 04:31:49 2009 From: tatiana.gonzalez <@t> leo-pharma.com (tatiana.gonzalez@leo-pharma.com) Date: Fri Jan 9 04:32:00 2009 Subject: [Histonet] Calcified aorta for reference purposes Message-ID: Does anyone know of a supplier where I can purchase slides with calcified tissue, preferably aortic tissue? I want to evaluate the degree of vascular calcification in an in house animal model and identify the most suitable staining for this model (alizarin, vonKossa or MacNeal). In order to implement existing protocols I need reference material. Can any of you out there help? Thanks, Tatiana Gonzalez This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. From njoydobro <@t> aol.com Fri Jan 9 04:41:57 2009 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Fri Jan 9 04:43:07 2009 Subject: [Histonet] Hematoxylin Counterstain for IHC slides In-Reply-To: <835298.41532.qm@web58604.mail.re3.yahoo.com> References: <835298.41532.qm@web58604.mail.re3.yahoo.com> Message-ID: <8CB405E07719937-BC0-E84@WEBMAIL-MZ03.sysops.aol.com> you know that TBS works for bluing any hematoxylin. Gene -----Original Message----- From: Kelly Boyd To: histonet Sent: Thu, 8 Jan 2009 2:46 pm Subject: Re: [Histonet] Hematoxylin Counterstain for IHC slides Biocare's Cat Hematoxylin is really good. One real nice feature is that you blue your slides in TBS. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 1/8/09, anh2006@med.cornell.edu wrote: From: anh2006@med.cornell.edu Subject: Re: [Histonet] Hematoxylin Counterstain for IHC slides To: "Ring, Mary L" , histonet@lists.utsouthwestern.edu Date: Thursday, January 8, 2009, 1:05 PM I use Dako Mayer's for 5 seconds followed by roughly a minute in bueing solution. Gorgeous blue. -----Original Message----- From: "Ring, Mary L" Date: Thu, 08 Jan 2009 09:50:00 To: Subject: [Histonet] Hematoxylin Counterstain for IHC slides Hello! I am looking for a good hematoxylin counterstain for IHC slides. My pathologists are familiar with the bright blue color of Ventana's hematoxylin counterstain, so I am looking for something similar. Thanks for your help! Mary ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histon et -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Fri Jan 9 07:12:10 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Jan 9 07:13:17 2009 Subject: [Histonet] RE: chromogens for triple immunohistochemistry Message-ID: Dear Anjan Kumar,IHC triple staining based on three different chromogens is possible with using various combinations of DAB (HRP), Vector VIP (HRP), Liquid Permanent Red (AP), Vector Blue, X-gal (beta-GAL). As long as the primary antibodies of interest do no show co-localization, you may end up with a reasonable staining result of three chromogens that (depending on the choice of chromogens) could be rather well discriminated from each other. However, as you said that all 'antigens of interest show co-localization', you do have a probem. Most chromogens that mix, do not have a distinct color that could be easy discriminated from both basic colors. The only way to solve this problem is the performance of spectral imaging analysis. Go to the Cambridge Research Instrumentation website for more info (www.cri-inc.com/products/nuance ). Under support/FAQs you will find a PDF (van der Loos pamphlet on multiple staining) that deals with the different chromogens for spectral imaging. Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 8 Jan 2009 17:05:52 +0530 From: anjan kumar Subject: [Histonet] chromogens for triple immunohistochemistry To: triple immunohistochem hi, I am very thankful to everyone for their replies to my questions. i had another one which is quite confusing and it is like i wanted to know which three colour chromogen combinations is best for triple immunohistochemistry. As all antigen of my interest are all co localized. regards, anjan kumar, Junior research scientist, Madras veterinary college. From kgreen <@t> hsh.org Fri Jan 9 07:27:43 2009 From: kgreen <@t> hsh.org (Green, Kathy) Date: Fri Jan 9 07:27:49 2009 Subject: [Histonet] uric acid Message-ID: We used to do a stain for uric acid, but we've discontinued that and now the pathologists only scrape it & put it on a slide with a coverslip on top to determine if there are urate crystals. My question is, is there a point to doing a special processing of the tissue from 100% to paraffin, if when we cut the H&E & put them on the stainer they go thru lesser alcohols/water anyway? Since they are doing their checking from a scarping now instead of the stain would it be appropriate to just process as normal or is the tissue somehow "preserved" better when we do the special processing on the tissue processors? Thanks for any help. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From S.J.Ainsworth <@t> bsms.ac.uk Fri Jan 9 07:55:42 2009 From: S.J.Ainsworth <@t> bsms.ac.uk (S.J.Ainsworth@bsms.ac.uk) Date: Fri Jan 9 07:56:52 2009 Subject: [Histonet] LacZ and cryosectioning In-Reply-To: <1505728483-1231439782-cardhu_decombobulator_blackberry.rim.net-295456701-@bxe294.bisx.prod.on.blackberry> References: <397EBB11B9C649428A1AB64BC16C66AA01911339@EXCHANGE2.university.brighton.ac.uk> <1505728483-1231439782-cardhu_decombobulator_blackberry.rim.net-295456701-@bxe294.bisx.prod.on.blackberry> Message-ID: <397EBB11B9C649428A1AB64BC16C66AA01945048@EXCHANGE2.university.brighton.ac.uk> The chick hindlimbs are removed and washed in chick ringer's solution (a salt solution to wash off and debris) then fresh frozen with cryo-m-bed. On the sections we fix with 2% PFA for 10mins, but the problem is arising before the sectioning. I am sure that the stain should be stronger as the staining on my positive controls that are frozen is massively reduced. Does that make any more sense? Thanks for your help Sophie Sophie Ainsworth Brighton and Sussex Medical school University of Sussex Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: 08 January 2009 18:34 To: Ainsworth Sophie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] LacZ and cryosectioning We do X-gal on snap frozen tissue all the time, so I can try to help. Can you give more specifics about your protocol? Your tissue is fresh frozen, correct? In OCT? Specifically what fixative are you using on your sections and for how long? Are you using Ca2+ and Mg2+ free PBS? Are you positive your tissue should be a strong stainer - if not do you have a sure-fire positive control? Andrea -----Original Message----- From: S.J.Ainsworth@bsms.ac.uk Date: Thu, 08 Jan 2009 17:17:41 To: Subject: [Histonet] LacZ and cryosectioning Hi, I?ve been having some trouble x-gal staining some cryosections of chick hindlimb tissue. I?ve been cutting 15 ?m sections then x-gal staining (using a protocol from another lab that should work) and counter staining with nuclear fast red. The sections look good with the nuclear fast red, but the x-gal staining only shows up as very faint speckling at x40. I routinely x-gal stain whole chick embryos then wax section and the blue of the x-gal stain is always very bright (both on the embryo before any tissue is removed for sectioning and in the wax sections). To try and find out if the problem was occurring before or after sectioning I defrosted some limbs (that had not as yet been cryosectioned) and x-gal stained them using the method that I use routinely, they showed none of the blue staining I would expect. Has anyone heard of snap freezing knocking down the activity of the ?-galactosidase enzyme or is able to suggest anything I could try to solve this, please? Thanks Sophie Sophie Ainsworth Brighton and Sussex Medical school University of Sussex Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 From tifei <@t> foxmail.com Fri Jan 9 08:06:26 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Jan 9 08:06:44 2009 Subject: [Histonet] Feulgen Nucleal Reaction Message-ID: <200901092206207721122@foxmail.com> Hi all, just wonder any one has the protocol for Feulgen Nucleal Reaction on thick brain sections, such as 20-40 um> I have one protocol refering to: http://stainsfile.info/StainsFile/stain/schiff/feulgen.htm 2009-01-09 TF From rcharles <@t> state.pa.us Fri Jan 9 08:13:41 2009 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Jan 9 08:14:40 2009 Subject: [Histonet] H&E help Message-ID: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From asmith <@t> mail.barry.edu Fri Jan 9 08:24:29 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Jan 9 08:26:01 2009 Subject: [Histonet] RE: H&E help In-Reply-To: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> References: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> Message-ID: Some tap waters blue hematoxylin very well, some not so well. I avoid the issue of differences in tap waters by blueing in 1.2% aqueous lithium carbonate (almost a saturated solution). - Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Fri Jan 9 09:22:30 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Jan 9 09:22:36 2009 Subject: [Histonet] Seeking Flow Cytometry Tech Message-ID: <450460.41146.qm@web57802.mail.re3.yahoo.com> We are currently seeking a flow cytometry technologist for our pathology laboratory in?Lakeland, Florida. Experience?and appropriate?state licensure is required. If interested,?send resume via e-mail or web site, www.micropathlabs.com. No recruiters please. Thank you, Sheila Haas Laboratory Supervisor Micro Path Laboratories From llewllew <@t> shaw.ca Fri Jan 9 10:01:52 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Jan 9 10:02:50 2009 Subject: [Histonet] H&E help References: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> Message-ID: Mayer's hemalum is a nuclear selective progressive formula so the staining is largely confined to nuclei. If the pathologists want more blue tinge in other parts of the tissue, then you should change the hemalum, or simply increase the hematoxylin content in the solution. Cole's does stain darker than Mayer's, but has 1.5 grams hematoxylin per litre, fully oxidised. Try that. You might want to decrease the time in eosin as well. Try one minute. ----- Original Message ----- From: "Charles, Roger" To: Sent: Friday, January 09, 2009 6:13 AM Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jan 9 10:07:29 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 9 10:14:14 2009 Subject: [Histonet] H&E help In-Reply-To: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> Message-ID: <975128.17941.qm@web65702.mail.ac4.yahoo.com> Roger: To rely in the "basic" quality of a tap water is a risky and inconsistent approach because not all tap waters are "created equal" and they could even vary along the year. My advise is that you use a basic solution, either a diluted (1-2%) ammonium hydroxyde or a 1-2% lithium carbonate solution to abtain a consistent blueing of Mayer (and any other hematoxylin for that matter). Ren? J. --- On Fri, 1/9/09, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] H&E help To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Friday, January 9, 2009, 9:13 AM Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdooley2008 <@t> yahoo.com Fri Jan 9 11:06:12 2009 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Fri Jan 9 11:09:25 2009 Subject: [Histonet] E16 paraffin embedding Message-ID: <421638.51857.qm@web45905.mail.sp1.yahoo.com> Hello All, I have been having trouble cutting whole embryo sections on E16 mice. I think the problem is with the fixation process. I am currently using a modified Carnoy's fixation (60% ETOH, 30% formaldehyde, 10% Glacial acetic acid). I would like to use this fixation as I have optimized my antibodies for this condition. The tissue is soft. I think it is due to incomplete dehydration. Does anyone have a any suggestions for doing tissue this large as I know over dehydrating can lead to problems also. Thank you, James From rjbuesa <@t> yahoo.com Fri Jan 9 13:46:10 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 9 13:47:08 2009 Subject: [Histonet] E16 paraffin embedding In-Reply-To: <421638.51857.qm@web45905.mail.sp1.yahoo.com> Message-ID: <400860.9395.qm@web65712.mail.ac4.yahoo.com> If your problem is sectioning, probably the problem is due to infiltration and the cause of the defective infiltration could be fixation, dehydration and/or clearing. Without knowing your protocol it is difficult to try to try to?find out the cause. Ren? J. --- On Fri, 1/9/09, James Dooley wrote: From: James Dooley Subject: [Histonet] E16 paraffin embedding To: histonet@lists.utsouthwestern.edu Date: Friday, January 9, 2009, 12:06 PM Hello All, I have been having trouble cutting whole embryo sections on E16 mice. I think the problem is with the fixation process. I am currently using a modified Carnoy's fixation (60% ETOH, 30% formaldehyde, 10% Glacial acetic acid). I would like to use this fixation as I have optimized my antibodies for this condition. The tissue is soft. I think it is due to incomplete dehydration. Does anyone have a any suggestions for doing tissue this large as I know over dehydrating can lead to problems also. Thank you, James _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Jan 9 16:28:37 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 9 16:28:53 2009 Subject: [Histonet] RE: Carnoy's fixation and E16 mouse embryos Message-ID: <000801c972a9$9d90c5c0$d8b25140$@callis@bresnan.net> James, You did not say how long you were fixing these somewhat large embryos? Nor did you give the processing schedule in detail, time/solvent/clearing agent/paraffin? Please provide this info, thanks! If the tissues are soft in the center, over dehydration is probably not the problem. It sounds as though fixation is incomplete, nor are you processing long enough (under-dehydration plus more) remove water, clear, and infiltrate with paraffin on these larger embryos OR both of the previous comments. If you let your larger embryos sit in this fixative (hopefully a very large quantity), then the acid may damage the antigens as compared to using this fixative for a shorter time with smaller, earlier embryos. We generally used Carnoys, the classic method with 10 ml acetic acid and 60 ml absolute ethanol and 40 ml chloroform to fix very THIN pieces of tissue overnight then rinse the fixative out with one change 95% ethanol to get rid of acetic acid and chloroform. The processing was started in 95% alcohol since the fixative also acts like a dehydrant. Acetic acid ruins red blood cells and could very well damage antigens over a long period of fixation. If you fixation is incomplete, then the graded alcohols during dehydration may complete the fixation anyway, not a good thing to have happen. However, your tissues are staying soft so inadequate fixation and dehydration may be factors. Penetration of the fixative can be improved if you slice skin over abdomen open to allow fixative into viscera, and also remove tails and appendages and even head if that is not of interest. In more recent years, we eliminated the chloroform (too carcinogenic/toxic) and used only 60 ml absolute ethanol, and 10 ml acetic acid since chloroform does NOT fix tissue, but is there to remove fats/lipids. Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT You wrote: I have been having trouble cutting whole embryo sections on E16 mice. I think the problem is with the fixation process. I am currently using a modified Carnoy's fixation (60% ETOH, 30% formaldehyde, 10% Glacial acetic acid). I would like to use this fixation as I have optimized my antibodies for this condition. The tissue is soft. I think it is due to incomplete dehydration. Does anyone have a any suggestions for doing tissue this large as I know over dehydrating can lead to problems also. From lpwenk <@t> sbcglobal.net Sat Jan 10 09:59:45 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jan 10 09:59:57 2009 Subject: [Histonet] H&E help In-Reply-To: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> Message-ID: It might not be a hematoxylin problem. It might be too much eosin. Try one of the following: - cut the eosin time down to 1 minute, and if it's still too pink/not blue enough, cut the eosin time down to 30 seconds. - and/or, change the first 95% alcohol after the eosin to 70% alcohol. That will help to put out more eosin than 95%. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Sat Jan 10 12:33:09 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sat Jan 10 12:34:10 2009 Subject: [Histonet] H&E help In-Reply-To: References: <1B2F365C5F88A946B7D602E64ACD9CDD09EEC8ADBA@ENHBGMBX04.PA.LCL> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5257F13@ITSSSXM01V6.one.ads.che.org> Also, warm water works best for bluing, if you have that option.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, January 10, 2009 11:00 AM To: 'Charles, Roger'; 'Histonet' Subject: RE: [Histonet] H&E help It might not be a hematoxylin problem. It might be too much eosin. Try one of the following: - cut the eosin time down to 1 minute, and if it's still too pink/not blue enough, cut the eosin time down to 30 seconds. - and/or, change the first 95% alcohol after the eosin to 70% alcohol. That will help to put out more eosin than 95%. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From sprin119 <@t> umn.edu Sun Jan 11 12:54:01 2009 From: sprin119 <@t> umn.edu (Jennifer Springsteen) Date: Sun Jan 11 12:53:59 2009 Subject: [Histonet] Re: E16 paraffin embedding Message-ID: <12c38a74d63bfdbb6c4e1fd746c34e82@umn.edu> When we process embryos this old, the are so large and their skin is so thick that we have to make a long slit down their back with a razor blade to allow infiltration. Otherwise, we get a soft, mushy mess that can't be sectioned. Depending on what you are interested, you could them in half down the midline, or do a slit down the back or belly. Good luck! -- Jennifer L. Springsteen, B.S. Lab Manager, Assistant Scientist Lillehei Heart Institute Histology & Microscopy Core Facility University of Minnesota School of Medicine, Division of Cardiology 312 Church Street SE 4-266 Nils Hasselmo Hall Minneapolis, MN ?55455 Lab: 612-626-3090 Cell: 651-357-7916 Fax: 612-624-8118 From tifei <@t> foxmail.com Sun Jan 11 20:23:06 2009 From: tifei <@t> foxmail.com (TF) Date: Sun Jan 11 20:23:21 2009 Subject: [Histonet] Fixation of the damaged brain tissue Message-ID: <200901121023012688143@foxmail.com> Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the injury). Because the injury damaged the surrounding blood supply, and after perfusion I normally got a mass of soft, shapeless tissue surrounding the injury site... I have also tried post-fixation of 24-48 hours before removing the brain from in situ. But it does not help a lot. Can anyone provide some suggestions? 2009-01-12 TF From eca9 <@t> georgetown.edu Mon Jan 12 08:35:15 2009 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Mon Jan 12 08:36:13 2009 Subject: [Histonet] bluing in tap water? Message-ID: <496B5523.60800@georgetown.edu> Good morning, I was wondering if someone uses tap water to blue their slides after Hematoxyline. If yes, do you use warm or cold water and for how long? Thanks, Eva Georgetown University From MLunetta <@t> luhcares.org Mon Jan 12 08:45:59 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon Jan 12 08:46:23 2009 Subject: [Histonet] Re: H&E Help Message-ID: <496AF537020000A800029830@mail.luhcares.org> You could also try to add some ammonium hydroxide in tap water after the Mayers and before the tap water rinse. This helps us in the bluing on our mayers H&E frozen tissue stain line. Add approx 5ml to 150ml of water from 30 sec to 1 min you will have to play with it a little . This blues our tissues up nicely. Matt Lunetta HT Longmont United Hospital Message: 1 Date: Sat, 10 Jan 2009 13:33:09 -0500 From: "Weems, Joyce" <~!B*+R^&>Subject: RE: [Histonet] H&E help To: , "Charles, Roger" ,<~!B*+R^&> "Histonet" <~!B*+R^&>Message-ID:<~!B*+R^&> <5D64396A0D4A5346BEBC759022AAEAA5257F13@ITSSSXM01V6.one.ads.che.org><~!B*+R^&>Content-Type: text/plain; charset="us-ascii" Also, warm water works best for bluing, if you have that option.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, January 10, 2009 11:00 AM To: 'Charles, Roger'; 'Histonet' Subject: RE: [Histonet] H&E help It might not be a hematoxylin problem. It might be too much eosin. Try one of the following: - cut the eosin time down to 1 minute, and if it's still too pink/not blue enough, cut the eosin time down to 30 seconds. - and/or, change the first 95% alcohol after the eosin to 70% alcohol. That will help to put out more eosin than 95%. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 13 **************************************** From contact <@t> excaliburpathology.com Mon Jan 12 08:51:56 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Jan 12 08:52:03 2009 Subject: [Histonet] bluing in tap water? Message-ID: <439200.7318.qm@web1104.biz.mail.sk1.yahoo.com> Hello, I rinse in tap water, but blue in 1-2% ammonium hydroxide for ~ minute, then rinse again in tap water. Paula ________________________________ From: Eva Permaul To: histonet@lists.utsouthwestern.edu Sent: Monday, January 12, 2009 8:35:15 AM Subject: [Histonet] bluing in tap water? Good morning, I was wondering if someone uses tap water to blue their slides after Hematoxyline. If yes, do you use warm or cold water and for how long? Thanks, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Jan 12 08:58:57 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jan 12 09:03:01 2009 Subject: [Histonet] Fixation of the damaged brain tissue In-Reply-To: <200901121023012688143@foxmail.com> References: <200901121023012688143@foxmail.com> Message-ID: <496B5AB1.1080603@umdnj.edu> Hi TF: Your problem should not be happening. Sounds like poor perfusion followed by immersing the whole brain (with the wound deep in the interior) in a small volume of fix at 4 degrees C. What fixative are you using? Geoff TF wrote: > Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the injury). > Because the injury damaged the surrounding blood supply, and after perfusion I normally got a mass of soft, shapeless tissue surrounding the injury site... > I have also tried post-fixation of 24-48 hours before removing the brain from in situ. But it does not help a lot. > Can anyone provide some suggestions? > > 2009-01-12 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Mon Jan 12 09:07:03 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 12 09:07:10 2009 Subject: [Histonet] bluing in tap water? In-Reply-To: <496B5523.60800@georgetown.edu> Message-ID: <997315.37052.qm@web65702.mail.ac4.yahoo.com> Bluing in tap water is quite often done but has the disadvantage that your protocol cannot be repeated in other Lab because not all tap waters have the same composition of "bluing quality". As any other chemical reaction it is better to blue in warm tap water (it will take less time) but you can also blue in cold water (at the temperature the water comes out of the tap). As to the time that will be determined by the "blue intensity" you are looking for and it will be less time in summer than in winter. All these intrinsic inconsistencies is what has determined the wide acceptance of the practice of bluing in a chemically prepared solution (like lithium carbonate or weak ammonia water). Ren? J.? --- On Mon, 1/12/09, Eva Permaul wrote: From: Eva Permaul Subject: [Histonet] bluing in tap water? To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 9:35 AM Good morning, I was wondering if someone uses tap water to blue their slides after Hematoxyline. If yes, do you use warm or cold water and for how long? Thanks, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Mon Jan 12 09:15:32 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Mon Jan 12 09:16:36 2009 Subject: FW: [Histonet] bluing in tap water? Message-ID: <1EC32117E0854F328E87EDEC26794037@IBLS.GLA.AC.UK> I blue in gently running tap water for the same time as I stained with the haematoxylin. Glasgow water is pure, gentle, and soft and comes from Loch Katrine. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: 12 January 2009 14:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bluing in tap water? Good morning, I was wondering if someone uses tap water to blue their slides after Hematoxyline. If yes, do you use warm or cold water and for how long? Thanks, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Mon Jan 12 09:29:37 2009 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Jan 12 09:29:42 2009 Subject: [Histonet] silver stain for mouse intestinal nerves Message-ID: <12133_1231774179_n0CFTcAW005422_OFC4D36734.448274A5-ON8525753C.0054805E-8525753C.00551C5E@notes.health.state.ny.us> I am looking for a silver stain method that would stain nerves in intestines of mice. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From tifei <@t> foxmail.com Mon Jan 12 09:41:46 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Mon Jan 12 09:42:52 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIEZpeGF0aW9uIG9mIHRoZSBkYW1hZ2VkIGJyYWluIHRpc3N1ZQ==?= References: <200901121023012688143@foxmail.com>, <496B5AB1.1080603@umdnj.edu> Message-ID: <200901122341408422100@foxmail.com> 4% PFA so u mean I should expose more of the damaged site (it is at the bottom of the brain, or olfactory bulb) and immerse the whole brain in a larger volume of fixatives? I think PFA penetrate quite fast, and I took all the skins & muscles off the skull before put the who brain with skull into PFA for 1-2 days. It should work... The perfusion is not well in many conditions - in my injury model, part of the blood supply was interrupted. 2009-01-12 TF ???? Geoff McAuliffe ????? 2009-01-12 22:59:22 ???? tifei ??? histonet ??? Re: [Histonet] Fixation of the damaged brain tissue Hi TF: Your problem should not be happening. Sounds like poor perfusion followed by immersing the whole brain (with the wound deep in the interior) in a small volume of fix at 4 degrees C. What fixative are you using? Geoff TF wrote: > Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the injury). > Because the injury damaged the surrounding blood supply, and after perfusion I normally got a mass of soft, shapeless tissue surrounding the injury site... > I have also tried post-fixation of 24-48 hours before removing the brain from in situ. But it does not help a lot. > Can anyone provide some suggestions? > > 2009-01-12 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From relia1 <@t> earthlink.net Mon Jan 12 09:47:45 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jan 12 09:47:49 2009 Subject: [Histonet] RELIA Histology Careers Bulletin 1-12-09 Happy New Year What do you resolve in 2009? Message-ID: What's your New Year's Resolution? Of course I resolve that I will try to eat right and exercise. I also intend to learn to play piano. But. my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2008 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. In 2009 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. Whatever you want to do; Wherever you want to be. Let Me Help! Free of Charge RELIA offers: * Assistance with updating or creating your resume * Tips on interviewing * Encouragement and Assistance during the course of your job search * Responsiveness - I will respond to your e-mails/phone calls within 24 hours or less. * Troubleshooting - if your job search is stalled or you can't get in the company you are interested in. * Personalized Job Search - customized to your experience, wants and needs. * Complete Confidentiality - Your career and my coaching is a relationship of trust. * Remember, If you know someone who might be interested I also offer a$500 referral bonus and if I place you a $500 hiring bonus. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. I have management positions in MA, TX, CA, WA, MD and NV. I have technician/technologist positions in PA, MD, DC, TX, WA, CA, MA, OH and NV. I have grossing histotech and PA positions in TX. Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don't worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Happy New Year and I hope to hear from you soon, Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From tifei <@t> foxmail.com Mon Jan 12 10:07:18 2009 From: tifei <@t> foxmail.com (TF) Date: Mon Jan 12 10:07:53 2009 Subject: =?utf-8?B?UmU6IFJFOiBSZTogW0hpc3RvbmV0XSBGaXhhdGlvbiBvZiB0aGUgZGFtYWdlZCBicmFpbiB0aXNzdWU=?= References: <200901121023012688143@foxmail.com>, <496B5AB1.1080603@umdnj.edu>, <200901122341408422100@foxmail.com>, Message-ID: <200901130007127468313@foxmail.com> Thanks, Dont you think high pressure during perfusion leads to tissue swelling and disrupted morphology? 2009-01-13 TF ???? Charles Scouten ????? 2009-01-13 00:02:40 ???? tifei@foxmail.com ??? histonet ??? RE: Re: [Histonet] Fixation of the damaged brain tissue Formaldehyde penetrates very slowly, that is why we do perfusion in the first place. To get fixative everywhere very quickly, since autolysis and breakdown starts almost immediately with anoxia. The tissue around the wound is decaying quickly, and not getting fixed fast enough. Consider using a higher pressure on the perfusion to force washout of the extra vascular space. Cordially, Charles W. Scouten, Ph.D Innovation Manager Biosystems Division Leica Biosystems St. Louis LLC 5918 Evergreen Blvd. St. Louis, MO 63134 United States of America telephone +1 314 522 0300 Ext. 342 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TF Sent: Monday, January 12, 2009 9:42 AM To: Geoff McAuliffe Cc: histonet Subject: Re: Re: [Histonet] Fixation of the damaged brain tissue 4% PFA so u mean I should expose more of the damaged site (it is at the bottom of the brain, or olfactory bulb) and immerse the whole brain in a larger volume of fixatives? I think PFA penetrate quite fast, and I took all the skins & muscles off the skull before put the who brain with skull into PFA for 1-2 days. It should work... The perfusion is not well in many conditions - in my injury model, part of the blood supply was interrupted. 2009-01-12 TF ???? Geoff McAuliffe ????? 2009-01-12 22:59:22 ???? tifei ??? histonet ??? Re: [Histonet] Fixation of the damaged brain tissue Hi TF: Your problem should not be happening. Sounds like poor perfusion followed by immersing the whole brain (with the wound deep in the interior) in a small volume of fix at 4 degrees C. What fixative are you using? Geoff TF wrote: > Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the injury). > Because the injury damaged the surrounding blood supply, and after perfusion I normally got a mass of soft, shapeless tissue surrounding the injury site... > I have also tried post-fixation of 24-48 hours before removing the brain from in situ. But it does not help a lot. > Can anyone provide some suggestions? > > 2009-01-12 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu **************** From NMargaryan <@t> childrensmemorial.org Mon Jan 12 10:07:34 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Jan 12 10:10:56 2009 Subject: [Histonet] RE: H&E help (Weems, Joyce) In-Reply-To: References: Message-ID: Hi Roger, I usually use Bluing reagent (Richard Allan Scientific, part of Fisher) after Hematoxylin and tap water, then wash again before eosin. Try it and you will see big difference. I just love it! All the best, Naira ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Jan 2009 13:33:09 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] H&E help To: , "Charles, Roger" , "Histonet" Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5257F13@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Also, warm water works best for bluing, if you have that option.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, January 10, 2009 11:00 AM To: 'Charles, Roger'; 'Histonet' Subject: RE: [Histonet] H&E help It might not be a hematoxylin problem. It might be too much eosin. Try one of the following: - cut the eosin time down to 1 minute, and if it's still too pink/not blue enough, cut the eosin time down to 30 seconds. - and/or, change the first 95% alcohol after the eosin to 70% alcohol. That will help to put out more eosin than 95%. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 13 **************************************** From asmith <@t> mail.barry.edu Mon Jan 12 10:19:40 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Jan 12 10:20:17 2009 Subject: [Histonet] silver stain for mouse intestinal nerves In-Reply-To: <12133_1231774179_n0CFTcAW005422_OFC4D36734.448274A5-ON8525753C.0054805E-8525753C.00551C5E@notes.health.state.ny.us> References: <12133_1231774179_n0CFTcAW005422_OFC4D36734.448274A5-ON8525753C.0054805E-8525753C.00551C5E@notes.health.state.ny.us> Message-ID: Almost any method will work in intestine. I have had good results with Holmes' stain and more impressive results with Kiernan's method. I have also had good results with Winkelmann's method, but it is terribly time consuming. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Monday, January 12, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain for mouse intestinal nerves I am looking for a silver stain method that would stain nerves in intestines of mice. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anne.lewin <@t> bms.com Mon Jan 12 11:04:26 2009 From: anne.lewin <@t> bms.com (Anne C Lewin) Date: Mon Jan 12 11:04:28 2009 Subject: [Histonet] bluing in tap water? In-Reply-To: <496B5523.60800@georgetown.edu> References: <496B5523.60800@georgetown.edu> Message-ID: <496B781A.40502@bms.com> When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beatrice.Debrosse-Serra <@t> pfizer.com Mon Jan 12 11:15:54 2009 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Mon Jan 12 11:16:05 2009 Subject: [Histonet] PATHOS Microwave Histoprocessor Message-ID: <7B41B921086ADE4186377B8C33F702DE07A63905@lajamrexm01.amer.pfizer.com> Hello Histonetters! We are in the process of purchasing a PATHOS Microwave Histoprocessor. What kind of experience did you have? How long were fixation/processing time in general? How happy were you with the results? We are a research lab and process mainly animal tissue. Any feedback would be greatly appreciated. Thank you in advance, Bea Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 From perron.b <@t> wanadoo.fr Mon Jan 12 12:02:05 2009 From: perron.b <@t> wanadoo.fr (Benjamin PERRON) Date: Mon Jan 12 12:02:10 2009 Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?) Message-ID: <14389681.292778.1231783325413.JavaMail.www@wwinf1a34> Hi all, I'm encountering problems while trying to do antigen retrieval (AR) on undecalcified bone (rat jaw) sections embedded in methylmetracrylate (MMA). Sections fall off the slides while heated. Section are 4?m thick, deposit on APES or gelatine coated slides, and heating is perform after total deplastification and rehydratation (I tried microwaves and warming bath in citrate buffer pH=6.0). Could some around here share theyre experience with AR on undecalcified bone sections and in particular tell me : -does section thickness as an impact on sections detachment while heating (I know some are performing thinner section, but 3-4?m is the thinner we are (in routine) able to do with rat jaw) -is theyre a particular coating for slides that would better fit that what we are using (APES or gelatine). Someone suggest me Poly-l-lysine. All tips and advices from experimented in this field would be very helpfull. Benjamin Perron Student (Master in Cell Biology) EA2496 Montrouge France From jp1000r <@t> hotmail.com Mon Jan 12 12:20:05 2009 From: jp1000r <@t> hotmail.com (JP Rey) Date: Mon Jan 12 12:22:54 2009 Subject: [Histonet] Technovit 9100 and partial vacuum Message-ID: Dear all, I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation step requires a "partial vaccum". Partial is everyting which is not total 100% (Absolute) so the rage is quite wide. Does any one using Technovit 9100 could tell which poucentage of vaccum to apply to the sample or how many Inches of Mercury could be read on the gauge? Here is a link to web site if you are using an other unit to evalute your vacuum. http://www.engineeringtoolbox.com/vacuum-converter-d_460.html Thank you for your help, Jean-Philippe REY (913) 213-2558 Jp1000r@hotmail.com From kgrobert <@t> rci.rutgers.edu Mon Jan 12 13:16:59 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Mon Jan 12 13:05:49 2009 Subject: [Histonet] Processing 3-dimensional cell cultures into paraffin Message-ID: <496B972B.4010000@rci.rutgers.edu> Histonetters, I just got a call from a researcher who is growing cells in soft agar in 3 dimensions and would like to process them into paraffin for sectioning, which I have never done before. I know she wants to preserve the 3D structure, and I found in the archives a protocol for putting cell pellets into agar for subsequent processing into paraffin, which gives me something to start with, sort of. Has anyone done this before? Can this be done on a processor (I have a Sakura VIP 5), or should I opt for manual processing? Either way, I would appreciate a processing protocol if anyone has one. Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Rutgers, the State University of NJ From MSHERWOOD <@t> PARTNERS.ORG Mon Jan 12 13:36:14 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Jan 12 13:37:15 2009 Subject: [Histonet] Re: Automatic Stainer/coverslipper Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2372F@PHSXMB30.partners.org> We are interested in purchasing an automatic stainer (and possible coverslipper). The Leica ST5020 model was recommended to us. Is anyone familiar with this stainer and/or Leica's CV5030 robotic coverslipper? The price for both is quite high and I want to make sure people are satisfied with it. We are a small research core lab whose histology needs are not as extensive as a clinical histology lab. Also, if anyone has another recommendation, I would be interested. Please reply directly to me. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Wanda.Smith <@t> HCAhealthcare.com Mon Jan 12 15:12:12 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Mon Jan 12 15:13:18 2009 Subject: [Histonet] bluing in tap water? In-Reply-To: <496B781A.40502@bms.com> References: <496B5523.60800@georgetown.edu> <496B781A.40502@bms.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362@NADCWPMSGCMS03.hca.corpad.net> What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Jan 12 15:56:48 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jan 12 15:57:04 2009 Subject: [Histonet] bluing in tap water? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362@NADCWPMSGCMS03.hca.corpad.net> Message-ID: I don't think you can blue too much. If the pH is too high then it can bleach the haematoxylin. Any mildly alkaline solution will do (in fact neutral tap water (pH 7) will slowly get there. The "special blueing" solutions available are many and varied: Warm tap water, phosphate buffer (pH7-8), a weak sodium hydroxide solution (< 0.5%), a lithium carbonate solution (saturated or a diluted form), a few drops of ammonia in water, Scott's blueing solution, etc. If you are worried about the alkalinity of your blueing solution check it with some pH strips. If the solution appears a slight pink (indicating that carry-over haematoxylin is in its acidic state) then the pH will be acidic and need replacing. How can you tell if the Haematoxylin is blued? Check microscopically. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Tuesday, 13 January 2009 8:12 AM To: Anne C Lewin; Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bluing in tap water? What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jamie.erickson <@t> abbott.com Mon Jan 12 16:10:29 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon Jan 12 16:11:42 2009 Subject: [Histonet] Rat and Mouse Bone histology methods and equipment--HELP Message-ID: Hello Histonetter, I hope someone out there in histoland can help me. I am about to be thrust (kicking and screaming) into the world of undecalcified bone histology and I need lots of help. My goals for 2009 will involve sectioning/staining and doing Image analysis on undecalcified rat and mouse bone samples. I am not completely unfamiliar with these methods but I learn these methods from some wise old, I mean experienced ladies that were specialists in bone histology. These ladies have now since retired and I am without any methods. I work in a small (2 histologist, 1 pathologist) histolab that does mouse and rat work and only decalcifed bone tissue. I have done plastic work in the past but that was 10 years ago in a land far, far away... If anyone out there could please refer me to a histology text books or share methods that would be of great help. If you do rat and mouse bone work and would be will to talk about methods/stains/ embedding/grinding/plastic processing/ image analysis that would be absolutely great. Thanks for any help you can provide.. Jamie _______________________________ Jamie Erickson Sr. Research Associate II Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From cathy <@t> wasatchhisto.com Mon Jan 12 16:28:00 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Jan 12 16:29:36 2009 Subject: [Histonet] lab closing, equipment for sale Message-ID: Fellow histonetters, I am retiring and closing my lab March 31, 2009. All lab equipment and consumables will be sold. There is equipment for ground histology, 3 automated microtomes, 1 paraffin microtome, 2 6 foot fume hoods, all peripheral equipment and consumables. There are also 10 brand new, never been used D-profile tungsten-carbide knives. There is too much to mention but please feel free to email me direct at Cathy@wasatchhisto.com for serious inquiries only. Cathy Mayton Wasatch Histo Consultants, Inc. From TanyaF <@t> adhb.govt.nz Mon Jan 12 19:56:13 2009 From: TanyaF <@t> adhb.govt.nz (Tanya Fulton) Date: Mon Jan 12 19:57:15 2009 Subject: [Histonet] Commercial Haematoxylin Message-ID: Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand From SwainFrancesL <@t> uams.edu Tue Jan 13 07:20:05 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Tue Jan 13 07:21:17 2009 Subject: [Histonet] Commercial Haematoxylin In-Reply-To: References: Message-ID: We use the hematoxylin's from PolyScientific R and D from BayTown New York. They are excellent. We use Harris', Weigert's, Mayer's and Gill's. They are reasonable in price and the quality is outstanding. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Fulton Sent: Monday, January 12, 2009 7:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Commercial Haematoxylin Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ratliffjack <@t> hotmail.com Tue Jan 13 07:45:14 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Jan 13 07:46:14 2009 Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?) In-Reply-To: <14389681.292778.1231783325413.JavaMail.www@wwinf1a34> References: <14389681.292778.1231783325413.JavaMail.www@wwinf1a34> Message-ID: Benjamin, How about trying Haupt's coated slides. I am not sure if these slides are now commercially available, but you can coat them yourself. In the past I have made the solution by scratch and also used the commercially prepared solution from Fisher. When you go to the Fisher Scientific website, the product will be listed under Harleco Gelatin Fixative #785-71 @ 500 mL. This is a concentrate solution, so it will be best to aliquot a small amount into a beaker, gently warm in a lab use only microwave for 10-20 seconds or a hot plate until the solution is in a complete liquid state, freely flowing. Then cut it 1:1 with 50% EtOH to prepare the working solution. Place 1-2 smal drops on a slide and use another slide of the same width to gently move the solution across the specimen mounting area. Finally, let it dry flat for 5-10 minutes and then you are ready to use. Let me know if you need anymore help! Jack Ratliff > From: perron.b@wanadoo.fr> To: histonet@lists.utsouthwestern.edu> Date: Mon, 12 Jan 2009 19:02:05 +0100> Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?)> > Hi all,> > I'm encountering problems while trying to do antigen retrieval (AR) on undecalcified bone (rat jaw) sections embedded in methylmetracrylate (MMA). Sections fall off the slides while heated.> Section are 4?m thick, deposit on APES or gelatine coated slides, and heating is perform after total deplastification and rehydratation (I tried microwaves and warming bath in citrate buffer pH=6.0).> > Could some around here share theyre experience with AR on undecalcified bone sections and in particular tell me :> > -does section thickness as an impact on sections detachment while heating (I know some are performing thinner section, but 3-4?m is the thinner we are (in routine) able to do with rat jaw)> -is theyre a particular coating for slides that would better fit that what we are using (APES or gelatine). Someone suggest me Poly-l-lysine.> > All tips and advices from experimented in this field would be very helpfull.> > Benjamin Perron> Student (Master in Cell Biology)> EA2496> Montrouge> France> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Jan 13 07:49:43 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Jan 13 07:50:41 2009 Subject: [Histonet] Technovit 9100 and partial vacuum In-Reply-To: References: Message-ID: JP, I believe that Technovit 9100 is pretty much a typical MMA formulation. If so, then I would add whatever vacuum you can safely add to the specimen. I use an MMA + DBP formulation (not a kit) and draw a vacuum @ -15 to -20 inHg. Let me know if you need anymore help! Jack Ratliff > From: jp1000r@hotmail.com> To: histonet@lists.utsouthwestern.edu> Date: Mon, 12 Jan 2009 12:20:05 -0600> Subject: [Histonet] Technovit 9100 and partial vacuum> > Dear all,> > > > I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation> step requires a "partial vaccum". Partial is everyting which is not total> 100% (Absolute) so the rage is quite wide.> > Does any one using Technovit 9100 could tell which poucentage of vaccum to> apply to the sample or how many Inches of Mercury could be read on the> gauge?> > > > Here is a link to web site if you are using an other unit to evalute your> vacuum.> > http://www.engineeringtoolbox.com/vacuum-converter-d_460.html> > > > > > Thank you for your help,> > > > > > Jean-Philippe REY> > (913) 213-2558> > Jp1000r@hotmail.com> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Tue Jan 13 08:25:38 2009 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Jan 13 08:25:44 2009 Subject: [Histonet] Job opportunity NJ Message-ID: <011320091425.18829.496CA462000910600000498D2216549976CCCECE9D0A0D0A99039D@comcast.net> I have an opening for a FT/PT or per diem histologist at a in office endoscopy laboratory in North New Jersey. This is a state of the art facility with new equipment that uses the latest in microwave specimen processing. We are seeking a experienced, ASCP certified histologist who is proficient in all aspects of GI specimen processing. Please send resume to above email address or call 732 814-1170 No headhunters please. Thank you From SARAH.REEVES <@t> ekht.nhs.uk Tue Jan 13 08:30:24 2009 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Tue Jan 13 08:32:36 2009 Subject: [Histonet] Mechanism of Staining - Special Stains Message-ID: <496CA580020000B500003867@ekhgwia.ekht.nhs.uk> Does anyone know of a good mechanism of staining reference for special stains? ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From rjbuesa <@t> yahoo.com Tue Jan 13 09:25:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 13 09:25:50 2009 Subject: [Histonet] Commercial Haematoxylin In-Reply-To: Message-ID: <239578.78507.qm@web65711.mail.ac4.yahoo.com> I was always very satisfied with Ricard Allan's hematoxylin. Ren? J. --- On Mon, 1/12/09, Tanya Fulton wrote: From: Tanya Fulton Subject: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 8:56 PM Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Jan 13 09:41:10 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jan 13 09:41:30 2009 Subject: [Histonet] Commercial Haematoxylin In-Reply-To: <239578.78507.qm@web65711.mail.ac4.yahoo.com> References: <239578.78507.qm@web65711.mail.ac4.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA52581EA@ITSSSXM01V6.one.ads.che.org> Richard Allen's 7211 is the most like the old Harris that I have come across. They started to discontinue it several years. I almost had heart failure! I wasn't the only one I guess, because they still have it. I called them immediately tho! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 13, 2009 10:26 AM To: histonet@lists.utsouthwestern.edu; Tanya Fulton Subject: Re: [Histonet] Commercial Haematoxylin I was always very satisfied with Ricard Allan's hematoxylin. Ren? J. --- On Mon, 1/12/09, Tanya Fulton wrote: From: Tanya Fulton Subject: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 8:56 PM Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From jessgrocki <@t> yahoo.com Tue Jan 13 09:51:15 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Jan 13 09:58:52 2009 Subject: [Histonet] Excelsior ES Tissue Processor Message-ID: <709115.94009.qm@web82001.mail.mud.yahoo.com> ? Good Morning: ? Our lab is looking into getting a new processor at some point!? We are looking at the Excelsior ES Tissue Processor from Thermo Scientific. We were wondering if any of you Histol labs out there are using this machine and if so how you like it? Have you had any problems with it, ie. mechanical?? Is it saving you money? and also is anyone using the machine with the xylene free option? ? Thanks so much! Have a great day! ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From mhamilton <@t> GENVEC.com Tue Jan 13 10:25:51 2009 From: mhamilton <@t> GENVEC.com (Hamilton, Melissa) Date: Tue Jan 13 10:29:42 2009 Subject: [Histonet] X-Gal staining Message-ID: <223C48015FDAC04E884941EBDB2F639C06DFB0@genexch.genvec.com> I am interested in any suggestions for what mouse tissue organ and route of administration that I could use as a postitive x-gal staining control to tease out tissue processing, staining and paraffin embedding artifacts. Regards, MH From tkngflght <@t> yahoo.com Tue Jan 13 10:51:44 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jan 13 10:51:26 2009 Subject: [Histonet] Commercial Haematoxylin In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA52581EA@ITSSSXM01V6.one.ads.che.org> References: <239578.78507.qm@web65711.mail.ac4.yahoo.com> <5D64396A0D4A5346BEBC759022AAEAA52581EA@ITSSSXM01V6.one.ads.che.org> Message-ID: We also always liked the Anatech version. Lasted forever, LOTS of heme in the solution so filtering didn't cause a problem---it's been a while since I've used it myself so you may want additional input. The only time we had trouble was when it sat on the loading dock and froze and thawed a couple of times before we got it---it took a while to figure it out but was easily remedied once we did. Hope this helps... Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 office 281.913.7288 fax hard line 281.883.7704 cell 800.756.3309 electronic fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, January 13, 2009 9:41 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Tanya Fulton Subject: RE: [Histonet] Commercial Haematoxylin Richard Allen's 7211 is the most like the old Harris that I have come across. They started to discontinue it several years. I almost had heart failure! I wasn't the only one I guess, because they still have it. I called them immediately tho! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 13, 2009 10:26 AM To: histonet@lists.utsouthwestern.edu; Tanya Fulton Subject: Re: [Histonet] Commercial Haematoxylin I was always very satisfied with Ricard Allan's hematoxylin. Ren? J. --- On Mon, 1/12/09, Tanya Fulton wrote: From: Tanya Fulton Subject: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 8:56 PM Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Tue Jan 13 11:02:51 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Tue Jan 13 11:05:25 2009 Subject: [Histonet] X-Gal staining Message-ID: <669371962-1231866316-cardhu_decombobulator_blackberry.rim.net-1085777367-@bxe277.bisx.prod.on.blackberry> The mouse epididymus has endogenous activity. However I suggest you use an animal transgenic for lacZ (any organ from a Rosa26 mouse for example). Sorry I do not understand what ypu mean by route of administration. ------Original Message------ From: Hamilton, Melissa Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Jan 13, 2009 11:25 AM Subject: [Histonet] X-Gal staining I am interested in any suggestions for what mouse tissue organ and route of administration that I could use as a postitive x-gal staining control to tease out tissue processing, staining and paraffin embedding artifacts. Regards, MH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Tue Jan 13 11:16:43 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Tue Jan 13 11:19:19 2009 Subject: [Histonet] bluing in tap water? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362@NADCWPMSGCMS03.hca.corpad.net> References: <496B5523.60800@georgetown.edu> <496B781A.40502@bms.com><9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <2022117152-1231867149-cardhu_decombobulator_blackberry.rim.net-1686426943-@bxe277.bisx.prod.on.blackberry> Is there such a thing as bluing too much? -----Original Message----- From: Smith Wanda Date: Mon, 12 Jan 2009 15:12:12 To: Anne C Lewin; Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bluing in tap water? What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HParker <@t> Skaggs.Net Tue Jan 13 11:37:02 2009 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Jan 13 11:36:59 2009 Subject: [Histonet] Spit and Diastase In-Reply-To: <20090107181152.7E29C37D1D4@barracuda.skaggs.net> Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker From PMonfils <@t> Lifespan.org Tue Jan 13 11:46:15 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jan 13 11:47:20 2009 Subject: [Histonet] Processing 3-dimensional cell cultures into paraffin In-Reply-To: <496B972B.4010000@rci.rutgers.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C83@LSRIEXCH1.lsmaster.lifespan.org> I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really isn't much different from processing and embedding tissue. You can trim the agar blocks to appropriate size with a scalpel (if necessary) either before or after fixation (if you trim them before fixation, do it while they are cold, so the agar is firm), and place them in a cassette just like a piece of tissue. Put them on the processor as usual, processing times the same as you would use for tissues of equivalent size, and embed as usual. One difference in sectioning however. Don't place the blocks in water after facing them off. The agar absorbs too much water and becomes soft. The blocks should be cut cold, but not moistened. Either place them on a cold plate without water, or put them in the refrigerator after facing, and take them out one at a time as you section them. From jqb7 <@t> cdc.gov Tue Jan 13 11:49:08 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Jan 13 11:49:20 2009 Subject: [Histonet] Processing 3-dimensional cell cultures into paraffin In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835C83@LSRIEXCH1.lsmaster.lifespan.org> References: <496B972B.4010000@rci.rutgers.edu> <4EBFF65383B74D49995298C4976D1D5E03835C83@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402AD8C@LTA3VS011.ees.hhs.gov> Histogel works great and does not absorb water like plain agar does. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Tuesday, January 13, 2009 12:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing 3-dimensional cell cultures into paraffin I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really isn't much different from processing and embedding tissue. You can trim the agar blocks to appropriate size with a scalpel (if necessary) either before or after fixation (if you trim them before fixation, do it while they are cold, so the agar is firm), and place them in a cassette just like a piece of tissue. Put them on the processor as usual, processing times the same as you would use for tissues of equivalent size, and embed as usual. One difference in sectioning however. Don't place the blocks in water after facing them off. The agar absorbs too much water and becomes soft. The blocks should be cut cold, but not moistened. Either place them on a cold plate without water, or put them in the refrigerator after facing, and take them out one at a time as you section them. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Jan 13 11:49:13 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jan 13 11:49:24 2009 Subject: [Histonet] Spit and Diastase In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C84@LSRIEXCH1.lsmaster.lifespan.org> Is she doing her PAS stains right after lunch? From mpence <@t> grhs.net Tue Jan 13 12:06:12 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jan 13 12:06:17 2009 Subject: [Histonet] Spit and Diastase In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3A35@IS-E2K3.grhs.net> I have seen the same thing happen if the person has recently ate or drank. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, January 13, 2009 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Spit and Diastase Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Tue Jan 13 12:01:39 2009 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Jan 13 12:09:17 2009 Subject: [Histonet] GSH meeting in March Message-ID: <2EA377B3F5CC4CAD941350DB09D8622B@powellsa1> Want to visit a warmer place? The Georgia Society for Histotechnology invites you to the 2009 meeting to be held at Sea Palms Resort at St. Simons Island, Georgia, March 20-22, 2009. The final program will be posted on our web site at www.histosearch.com/gsh soon as finalized. Please call for reservations now by calling the Sea Palms Resort and be sure to tell them you are attending the GSH meeting. The phone number is 1-800-841-6268. The address is 5445 Frederica Road, St. Simons Island, GA 31522. Ph: 912-638-3351. Fax-912-638-5416 and web site is www.seapalms.com Special GSH Room Rates are $99 for two double beds, an oversized bath, sitting area, a wet bar with a small refrigerator and Screened-in porch or sunroom $109 for two double beds or a King bed, an oversized bath, wet bar refrigerator, two burner stove, microwave and sunroom. Suites are available as well as Villas. Go to their web site to see the room layouts. Ask for GSH prices on the suites and villas. The Sea Palms Resort is one of Georgia's most unique resorts and is renowned as one of the East Coast's finest golf and tennis getaways. . 27 holes of golf surrounding our facilities. . Health & Racquet Facility, 2 large swimming pools, 2 sand volleyball courts . Just a few miles from our beach club. . Conference Center, offering over 7500 square feet of flexible meeting space. In close proximity to Sea Palms Resort on St. Simons Island there are many activities your group can enjoy, to name a few: . St. Simons Historic Tours featuring Fort Frederica . Fishing, Horseback Riding, Parasailing, Wave Runners, Sailing and Beach Cruisers. . Shopping Tours, Antique shops, Shops at Sea Island, and the St. Simons Pier Village. AIRPORT TRANSPORTATION: Sea Palms Resort will provide transportation as follows. One way to Jacksonville and Savannah Airport $95 first 2 people and $25 each additional per person per trip. One way to Brunswick $35 first 2 people and $15 each additional person per trip. BEACH CLUB: All guests of Sea Palms may enjoy unlimited access to the St. Simons Beach Club, located right on the Atlantic Ocean and just a four mile drive from the resort. The luxurious facility offers visitors private beach access, a swimming pool, Jacuzzi tub, beach chair rentals and snack bar options in season. Plan your family vacation now. Come to Georgia and experience the famous Southern Hospitality. Shirley Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 Ph: 478-301-2374 Fx: 478-301-5489 From sheila_adey <@t> hotmail.com Tue Jan 13 12:17:01 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 13 12:17:59 2009 Subject: [Histonet] Time & temp in oven prior to IHCs ? Message-ID: Good afternoon Netters, Our prostate core biopsies have been "floating" off during the IHCs. We are wondering how long other institutions are drying the slides prior to going to water? We typically dry for 25 min at 65 degrees and we use charged slides. These slides are retrieved in the microwave. Thanks in advance for your input. :)Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ From bob.nienhuis <@t> gmail.com Tue Jan 13 12:21:06 2009 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Tue Jan 13 12:21:13 2009 Subject: [Histonet] Histogel Message-ID: <45109da50901131021k6e5e0552y397ef15ac85ea53d@mail.gmail.com> Has anyone tried embedding a block of tissue like a mouse brain in Histogel, and then cutting frozen or on a vibratome? I am doing Golgi stain on mouse brain, and the tissue is VERY friable. Website says, "No matter how small, friable, or viscous your histology or cytology specimen, HistoGel will encapsulate and retain the entire specimen during histological processing" Bob Nienhuis UCLA / VA Medical Center On Tue, Jan 13, 2009 at 9:49 AM, Bartlett, Jeanine (CDC/CCID/NCZVED) < jqb7@cdc.gov> wrote: > Histogel works great and does not absorb water like plain agar does. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, > Paul > Sent: Tuesday, January 13, 2009 12:46 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Processing 3-dimensional cell cultures into > paraffin > > I have processed and embedded cells grown in/on agar, or post-embedded > in agar or agarose, many times. It really isn't much different from > processing and embedding tissue. You can trim the agar blocks to > appropriate size with a scalpel (if necessary) either before or after > fixation (if you trim them before fixation, do it while they are cold, > so the agar is firm), and place them in a cassette just like a piece of > tissue. Put them on the processor as usual, processing times the same as > you would use for tissues of equivalent size, and embed as usual. One > difference in sectioning however. Don't place the blocks in water after > facing them off. The agar absorbs too much water and becomes soft. The > blocks should be cut cold, but not moistened. Either place them on a > cold plate without water, or put them in the refrigerator after facing, > and take them out one at a time as you section them. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jodiputnam <@t> aol.com Tue Jan 13 12:23:14 2009 From: jodiputnam <@t> aol.com (jodiputnam@aol.com) Date: Tue Jan 13 12:24:49 2009 Subject: [Histonet] Tallahassee Florida area Message-ID: <8CB43C321FE2F10-A1C-88A@WEBMAIL-MC21.sysops.aol.com> Hi am posting a message for a former coworker. He is moving to the Tallahassee area within the month and I told him I would put out a few feelers to see if there were any employers hiring in that area or surrounding areas. He has worked in pathology for ~19 years, ran the gross room at a large hospital and has experience in the cytology prep area also. He is familiar with several accessioning programs and billing and coding. Thanks for any info. Jodi Putnam From nancy.troiano <@t> yale.edu Tue Jan 13 12:32:12 2009 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Tue Jan 13 12:32:23 2009 Subject: [Histonet] Antigen Retrieval on MMA slides Message-ID: <5.2.1.1.2.20090113132938.022929e0@email.med.yale.edu> We put our plastic sections (5-6 microns in thickness) onto slides manufactured by Scientific Device Laboratories, the "in situ plus" slides. We then bake the sections overnight in a 60 degree oven, then bring to room temperature before doing immunostaining. Sections will not stay on gel coated slides and the gelatin on the slides will interfere with your immunostaining. From tbraud <@t> holyredeemer.com Tue Jan 13 12:36:49 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 13 12:37:48 2009 Subject: [Histonet] RE: Commercial Hematoxylin In-Reply-To: <87d5bbce001248c1@HolyRedeemer.com> Message-ID: I echo the sentiments on the great quality of Richard Allen's Hematoxylin 7211. I've used many good commercial Hematoxylins (Shandon used to make an excellent Gill) but the stability of the 7211 is incredible! Also, during the recent Hematoxylin Shortage, I had no problems with obtaining the product. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 9. Commercial Haematoxylin (Tanya Fulton) Message: 9 Date: Tue, 13 Jan 2009 14:56:13 +1300 From: "Tanya Fulton" Subject: [Histonet] Commercial Haematoxylin To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Wanda.Smith <@t> HCAhealthcare.com Tue Jan 13 12:14:01 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Jan 13 12:38:19 2009 Subject: [Histonet] bluing in tap water? In-Reply-To: <2022117152-1231867149-cardhu_decombobulator_blackberry.rim.net-1686426943-@bxe277.bisx.prod.on.blackberry> References: <496B5523.60800@georgetown.edu> <496B781A.40502@bms.com><9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362@NADCWPMSGCMS03.hca.corpad.net> <2022117152-1231867149-cardhu_decombobulator_blackberry.rim.net-1686426943-@bxe277.bisx.prod.on.blackberry> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC498F@NADCWPMSGCMS03.hca.corpad.net> We were having issues with the "blue haze" on the rest of the slide and from the archives, the cause of blue haze can be water that is too cold. I also thought the pH of the running tap water in the automatic stainer could be a cause also. That's what I meant by to blue. Thanks for the info, W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Tuesday, January 13, 2009 12:17 PM To: Smith Wanda; Anne C Lewin; Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? Is there such a thing as bluing too much? -----Original Message----- From: Smith Wanda Date: Mon, 12 Jan 2009 15:12:12 To: Anne C Lewin; Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bluing in tap water? What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JonSorenson <@t> chiwest.com Tue Jan 13 12:45:19 2009 From: JonSorenson <@t> chiwest.com (Sorenson, Jon (Nampa)) Date: Tue Jan 13 12:46:08 2009 Subject: [Histonet] RE: Histonet Digest, Vol 62, Issue 15 References: <20090113182042.EBF4CB7AJV@email3.catholichealth.net> Message-ID: <55F887698A32F54F8918ABC6952E0D9D010A1966@CHIMSX02.CHI.catholichealth.net> Is the person involved a smoker? We have had trouble with the saliva of people that smoke not having diastase in the past, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com ________________________________ From jmartin <@t> lrgh.org Tue Jan 13 12:45:15 2009 From: jmartin <@t> lrgh.org (Martin, Jessica) Date: Tue Jan 13 12:48:54 2009 Subject: [Histonet] RE: Histonet Digest, Vol 62, Issue 15 In-Reply-To: <1c0fc052-f176-42a9-9f46-caa7917dd98a@LRGHEXEDGE1.lrgh.org> References: <1c0fc052-f176-42a9-9f46-caa7917dd98a@LRGHEXEDGE1.lrgh.org> Message-ID: <53ADC5744236FC43B956FBCA32DEBBE30138DB785E@LRGHEXVS2.practice.lrgh.org> Good Morning: ? Our lab is looking into getting a new processor at some point!? We are looking at the Excelsior ES Tissue Processor from Thermo Scientific. We were wondering if any of you Histol labs out there are using this machine and if so how you like it? Have you had any problems with it, ie. mechanical?? Is it saving you money? and also is anyone using the machine with the xylene free option? ? Thanks so much! Have a great day! ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital We have been using the Excelsior for about two years now. It has been great. I would recommend it highly. We have not tried the xylene free processing yet. There have been a few mechanical problems here and there but I feel that is to be expected of any piece of equipment. This processor has saved us a ton of money as the reagents don't have to be changed as often. Try a demo of one I don't think you will be disappointed. Jessica Martin HT, ASCP (603) 524-3211 x-3231 jmartin@lrgh.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, January 13, 2009 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 62, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Undecalcified bone in MMA and antigen retrieval (Neil Hand?) (Benjamin PERRON) 2. Technovit 9100 and partial vacuum (JP Rey) 3. Processing 3-dimensional cell cultures into paraffin (Kathleen Roberts) 4. Re: Automatic Stainer/coverslipper (Sherwood, Margaret ) 5. RE: bluing in tap water? (Smith Wanda) 6. RE: bluing in tap water? (Tony Henwood) 7. Rat and Mouse Bone histology methods and equipment--HELP (Jamie E Erickson) 8. lab closing, equipment for sale (Cathy Mayton) 9. Commercial Haematoxylin (Tanya Fulton) 10. RE: Commercial Haematoxylin (Swain, Frances L) 11. RE: Undecalcified bone in MMA and antigen retrieval (Neil Hand?) (Jack Ratliff) 12. RE: Technovit 9100 and partial vacuum (Jack Ratliff) 13. Job opportunity NJ (rmweber113@comcast.net) 14. Mechanism of Staining - Special Stains (SARAH REEVES) 15. Re: Commercial Haematoxylin (Rene J Buesa) 16. RE: Commercial Haematoxylin (Weems, Joyce) 17. Excelsior ES Tissue Processor (Jessica Piche) 18. X-Gal staining (Hamilton, Melissa) 19. RE: Commercial Haematoxylin (Cheryl) 20. Re: X-Gal staining (anh2006@med.cornell.edu) 21. Re: bluing in tap water? (anh2006@med.cornell.edu) 22. Spit and Diastase (Parker, Helayne) 23. RE: Processing 3-dimensional cell cultures into paraffin (Monfils, Paul) 24. RE: Processing 3-dimensional cell cultures into paraffin (Bartlett, Jeanine (CDC/CCID/NCZVED)) 25. RE: Spit and Diastase (Monfils, Paul) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Jan 2009 19:02:05 +0100 (CET) From: Benjamin PERRON Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?) To: histonet@lists.utsouthwestern.edu Message-ID: <14389681.292778.1231783325413.JavaMail.www@wwinf1a34> Content-Type: text/plain; charset=UTF-8 Hi all, I'm encountering problems while trying to do antigen retrieval (AR) on undecalcified bone (rat jaw) sections embedded in methylmetracrylate (MMA). Sections fall off the slides while heated. Section are 4??m thick, deposit on APES or gelatine coated slides, and heating is perform after total deplastification and rehydratation (I tried microwaves and warming bath in citrate buffer pH=6.0). Could some around here share theyre experience with AR on undecalcified bone sections and in particular tell me : -does section thickness as an impact on sections detachment while heating (I know some are performing thinner section, but 3-4??m is the thinner we are (in routine) able to do with rat jaw) -is theyre a particular coating for slides that would better fit that what we are using (APES or gelatine). Someone suggest me Poly-l-lysine. All tips and advices from experimented in this field would be very helpfull. Benjamin Perron Student (Master in Cell Biology) EA2496 Montrouge France ------------------------------ Message: 2 Date: Mon, 12 Jan 2009 12:20:05 -0600 From: "JP Rey" Subject: [Histonet] Technovit 9100 and partial vacuum To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear all, I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation step requires a "partial vaccum". Partial is everyting which is not total 100% (Absolute) so the rage is quite wide. Does any one using Technovit 9100 could tell which poucentage of vaccum to apply to the sample or how many Inches of Mercury could be read on the gauge? Here is a link to web site if you are using an other unit to evalute your vacuum. http://www.engineeringtoolbox.com/vacuum-converter-d_460.html Thank you for your help, Jean-Philippe REY (913) 213-2558 Jp1000r@hotmail.com ------------------------------ Message: 3 Date: Mon, 12 Jan 2009 14:16:59 -0500 From: Kathleen Roberts Subject: [Histonet] Processing 3-dimensional cell cultures into paraffin To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <496B972B.4010000@rci.rutgers.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Histonetters, I just got a call from a researcher who is growing cells in soft agar in 3 dimensions and would like to process them into paraffin for sectioning, which I have never done before. I know she wants to preserve the 3D structure, and I found in the archives a protocol for putting cell pellets into agar for subsequent processing into paraffin, which gives me something to start with, sort of. Has anyone done this before? Can this be done on a processor (I have a Sakura VIP 5), or should I opt for manual processing? Either way, I would appreciate a processing protocol if anyone has one. Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Rutgers, the State University of NJ ------------------------------ Message: 4 Date: Mon, 12 Jan 2009 14:36:14 -0500 From: "Sherwood, Margaret " Subject: [Histonet] Re: Automatic Stainer/coverslipper To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E2372F@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We are interested in purchasing an automatic stainer (and possible coverslipper). The Leica ST5020 model was recommended to us. Is anyone familiar with this stainer and/or Leica's CV5030 robotic coverslipper? The price for both is quite high and I want to make sure people are satisfied with it. We are a small research core lab whose histology needs are not as extensive as a clinical histology lab. Also, if anyone has another recommendation, I would be interested. Please reply directly to me. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 5 Date: Mon, 12 Jan 2009 15:12:12 -0600 From: Smith Wanda Subject: RE: [Histonet] bluing in tap water? To: Anne C Lewin , Eva Permaul Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362@NADCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 13 Jan 2009 08:56:48 +1100 From: "Tony Henwood" Subject: RE: [Histonet] bluing in tap water? To: "Smith Wanda" , "Anne C Lewin" , "Eva Permaul" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" I don't think you can blue too much. If the pH is too high then it can bleach the haematoxylin. Any mildly alkaline solution will do (in fact neutral tap water (pH 7) will slowly get there. The "special blueing" solutions available are many and varied: Warm tap water, phosphate buffer (pH7-8), a weak sodium hydroxide solution (< 0.5%), a lithium carbonate solution (saturated or a diluted form), a few drops of ammonia in water, Scott's blueing solution, etc. If you are worried about the alkalinity of your blueing solution check it with some pH strips. If the solution appears a slight pink (indicating that carry-over haematoxylin is in its acidic state) then the pH will be acidic and need replacing. How can you tell if the Haematoxylin is blued? Check microscopically. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Tuesday, 13 January 2009 8:12 AM To: Anne C Lewin; Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bluing in tap water? What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 7 Date: Mon, 12 Jan 2009 17:10:29 -0500 From: Jamie E Erickson Subject: [Histonet] Rat and Mouse Bone histology methods and equipment--HELP To: "histonet histonet" Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello Histonetter, I hope someone out there in histoland can help me. I am about to be thrust (kicking and screaming) into the world of undecalcified bone histology and I need lots of help. My goals for 2009 will involve sectioning/staining and doing Image analysis on undecalcified rat and mouse bone samples. I am not completely unfamiliar with these methods but I learn these methods from some wise old, I mean experienced ladies that were specialists in bone histology. These ladies have now since retired and I am without any methods. I work in a small (2 histologist, 1 pathologist) histolab that does mouse and rat work and only decalcifed bone tissue. I have done plastic work in the past but that was 10 years ago in a land far, far away... If anyone out there could please refer me to a histology text books or share methods that would be of great help. If you do rat and mouse bone work and would be will to talk about methods/stains/ embedding/grinding/plastic processing/ image analysis that would be absolutely great. Thanks for any help you can provide.. Jamie _______________________________ Jamie Erickson Sr. Research Associate II Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com ------------------------------ Message: 8 Date: Mon, 12 Jan 2009 14:28:00 -0800 From: "Cathy Mayton" Subject: [Histonet] lab closing, equipment for sale To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Fellow histonetters, I am retiring and closing my lab March 31, 2009. All lab equipment and consumables will be sold. There is equipment for ground histology, 3 automated microtomes, 1 paraffin microtome, 2 6 foot fume hoods, all peripheral equipment and consumables. There are also 10 brand new, never been used D-profile tungsten-carbide knives. There is too much to mention but please feel free to email me direct at Cathy@wasatchhisto.com for serious inquiries only. Cathy Mayton Wasatch Histo Consultants, Inc. ------------------------------ Message: 9 Date: Tue, 13 Jan 2009 14:56:13 +1300 From: "Tanya Fulton" Subject: [Histonet] Commercial Haematoxylin To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand ------------------------------ Message: 10 Date: Tue, 13 Jan 2009 07:20:05 -0600 From: "Swain, Frances L" Subject: RE: [Histonet] Commercial Haematoxylin To: "Tanya Fulton" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii We use the hematoxylin's from PolyScientific R and D from BayTown New York. They are excellent. We use Harris', Weigert's, Mayer's and Gill's. They are reasonable in price and the quality is outstanding. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Fulton Sent: Monday, January 12, 2009 7:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Commercial Haematoxylin Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 11 Date: Tue, 13 Jan 2009 08:45:14 -0500 From: Jack Ratliff Subject: RE: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?) To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Benjamin, How about trying Haupt's coated slides. I am not sure if these slides are now commercially available, but you can coat them yourself. In the past I have made the solution by scratch and also used the commercially prepared solution from Fisher. When you go to the Fisher Scientific website, the product will be listed under Harleco Gelatin Fixative #785-71 @ 500 mL. This is a concentrate solution, so it will be best to aliquot a small amount into a beaker, gently warm in a lab use only microwave for 10-20 seconds or a hot plate until the solution is in a complete liquid state, freely flowing. Then cut it 1:1 with 50% EtOH to prepare the working solution. Place 1-2 smal drops on a slide and use another slide of the same width to gently move the solution across the specimen mounting area. Finally, let it dry flat for 5-10 minutes and then you are ready to use. Let me know if you need anymore help! Jack Ratliff > From: perron.b@wanadoo.fr> To: histonet@lists.utsouthwestern.edu> Date: Mon, 12 Jan 2009 19:02:05 +0100> Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?)> > Hi all,> > I'm encountering problems while trying to do antigen retrieval (AR) on undecalcified bone (rat jaw) sections embedded in methylmetracrylate (MMA). Sections fall off the slides while heated.> Section are 4?m thick, deposit on APES or gelatine coated slides, and heating is perform after total deplastification and rehydratation (I tried microwaves and warming bath in citrate buffer pH=6.0).> > Could some around here share theyre experience with AR on undecalcified bone sections and in particular tell me :> > -does section thickness as an impact on sections detachment while heating (I know some are performing thinner section, but 3-4?m is the thinner we are (in routine) able to do with rat jaw)> -is theyre a particular coating for slides that would better fit that what we are using (APES or gelatine). Someone suggest me Poly-l-lysine.> > All tips and advices from experimented in this field would be very helpfull.> > Benjamin Perron> Student (Master in Cell Biology)> EA2496> Montrouge> France> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 13 Jan 2009 08:49:43 -0500 From: Jack Ratliff Subject: RE: [Histonet] Technovit 9100 and partial vacuum To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" JP, I believe that Technovit 9100 is pretty much a typical MMA formulation. If so, then I would add whatever vacuum you can safely add to the specimen. I use an MMA + DBP formulation (not a kit) and draw a vacuum @ -15 to -20 inHg. Let me know if you need anymore help! Jack Ratliff > From: jp1000r@hotmail.com> To: histonet@lists.utsouthwestern.edu> Date: Mon, 12 Jan 2009 12:20:05 -0600> Subject: [Histonet] Technovit 9100 and partial vacuum> > Dear all,> > > > I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation> step requires a "partial vaccum". Partial is everyting which is not total> 100% (Absolute) so the rage is quite wide.> > Does any one using Technovit 9100 could tell which poucentage of vaccum to> apply to the sample or how many Inches of Mercury could be read on the> gauge?> > > > Here is a link to web site if you are using an other unit to evalute your> vacuum.> > http://www.engineeringtoolbox.com/vacuum-converter-d_460.html> > > > > > Thank you for your help,> > > > > > Jean-Philippe REY> > (913) 213-2558> > Jp1000r@hotmail.com> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 13 Jan 2009 14:25:38 +0000 From: rmweber113@comcast.net Subject: [Histonet] Job opportunity NJ To: histonet@lists.utsouthwestern.edu Message-ID: <011320091425.18829.496CA462000910600000498D2216549976CCCECE9D0A0D0A99039D@comcast.net> Content-Type: text/plain I have an opening for a FT/PT or per diem histologist at a in office endoscopy laboratory in North New Jersey. This is a state of the art facility with new equipment that uses the latest in microwave specimen processing. We are seeking a experienced, ASCP certified histologist who is proficient in all aspects of GI specimen processing. Please send resume to above email address or call 732 814-1170 No headhunters please. Thank you ------------------------------ Message: 14 Date: Tue, 13 Jan 2009 14:30:24 +0000 From: "SARAH REEVES" Subject: [Histonet] Mechanism of Staining - Special Stains To: ", Message-ID: <496CA580020000B500003867@ekhgwia.ekht.nhs.uk> Content-Type: text/plain; charset="us-ascii" Does anyone know of a good mechanism of staining reference for special stains? ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** ------------------------------ Message: 15 Date: Tue, 13 Jan 2009 07:25:47 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu, Tanya Fulton Message-ID: <239578.78507.qm@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I was always very satisfied with Ricard Allan's hematoxylin. Ren? J. --- On Mon, 1/12/09, Tanya Fulton wrote: From: Tanya Fulton Subject: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 8:56 PM Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 13 Jan 2009 10:41:10 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Commercial Haematoxylin To: , , "Tanya Fulton" Message-ID: <5D64396A0D4A5346BEBC759022AAEAA52581EA@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Richard Allen's 7211 is the most like the old Harris that I have come across. They started to discontinue it several years. I almost had heart failure! I wasn't the only one I guess, because they still have it. I called them immediately tho! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 13, 2009 10:26 AM To: histonet@lists.utsouthwestern.edu; Tanya Fulton Subject: Re: [Histonet] Commercial Haematoxylin I was always very satisfied with Ricard Allan's hematoxylin. Ren? J. --- On Mon, 1/12/09, Tanya Fulton wrote: From: Tanya Fulton Subject: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 8:56 PM Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 17 Date: Tue, 13 Jan 2009 07:51:15 -0800 (PST) From: Jessica Piche Subject: [Histonet] Excelsior ES Tissue Processor To: histonet Message-ID: <709115.94009.qm@web82001.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? Good Morning: ? Our lab is looking into getting a new processor at some point!? We are looking at the Excelsior ES Tissue Processor from Thermo Scientific. We were wondering if any of you Histol labs out there are using this machine and if so how you like it? Have you had any problems with it, ie. mechanical?? Is it saving you money? and also is anyone using the machine with the xylene free option? ? Thanks so much! Have a great day! ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ------------------------------ Message: 18 Date: Tue, 13 Jan 2009 11:25:51 -0500 From: "Hamilton, Melissa" Subject: [Histonet] X-Gal staining To: Message-ID: <223C48015FDAC04E884941EBDB2F639C06DFB0@genexch.genvec.com> Content-Type: text/plain; charset="us-ascii" I am interested in any suggestions for what mouse tissue organ and route of administration that I could use as a postitive x-gal staining control to tease out tissue processing, staining and paraffin embedding artifacts. Regards, MH ------------------------------ Message: 19 Date: Tue, 13 Jan 2009 10:51:44 -0600 From: "Cheryl" Subject: RE: [Histonet] Commercial Haematoxylin To: "'Weems, Joyce'" , , , "'Tanya Fulton'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We also always liked the Anatech version. Lasted forever, LOTS of heme in the solution so filtering didn't cause a problem---it's been a while since I've used it myself so you may want additional input. The only time we had trouble was when it sat on the loading dock and froze and thawed a couple of times before we got it---it took a while to figure it out but was easily remedied once we did. Hope this helps... Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 office 281.913.7288 fax hard line 281.883.7704 cell 800.756.3309 electronic fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, January 13, 2009 9:41 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Tanya Fulton Subject: RE: [Histonet] Commercial Haematoxylin Richard Allen's 7211 is the most like the old Harris that I have come across. They started to discontinue it several years. I almost had heart failure! I wasn't the only one I guess, because they still have it. I called them immediately tho! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 13, 2009 10:26 AM To: histonet@lists.utsouthwestern.edu; Tanya Fulton Subject: Re: [Histonet] Commercial Haematoxylin I was always very satisfied with Ricard Allan's hematoxylin. Ren? J. --- On Mon, 1/12/09, Tanya Fulton wrote: From: Tanya Fulton Subject: [Histonet] Commercial Haematoxylin To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 8:56 PM Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated. Tanya LabPLUS Auckland City Hospital New Zealand _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 13 Jan 2009 17:02:51 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] X-Gal staining To: "Hamilton, Melissa" , histonet@lists.utsouthwestern.edu Message-ID: <669371962-1231866316-cardhu_decombobulator_blackberry.rim.net-1085777367-@bxe277.bisx.prod.on.blackberry> Content-Type: text/plain The mouse epididymus has endogenous activity. However I suggest you use an animal transgenic for lacZ (any organ from a Rosa26 mouse for example). Sorry I do not understand what ypu mean by route of administration. ------Original Message------ From: Hamilton, Melissa Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Jan 13, 2009 11:25 AM Subject: [Histonet] X-Gal staining I am interested in any suggestions for what mouse tissue organ and route of administration that I could use as a postitive x-gal staining control to tease out tissue processing, staining and paraffin embedding artifacts. Regards, MH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 13 Jan 2009 17:16:43 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] bluing in tap water? To: "Smith Wanda" , "Anne C Lewin" , "Eva Permaul" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <2022117152-1231867149-cardhu_decombobulator_blackberry.rim.net-1686426943-@bxe277.bisx.prod.on.blackberry> Content-Type: text/plain Is there such a thing as bluing too much? -----Original Message----- From: Smith Wanda Date: Mon, 12 Jan 2009 15:12:12 To: Anne C Lewin; Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bluing in tap water? What should the pH of tap water be to blue just right and not too much??? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin Sent: Monday, January 12, 2009 12:04 PM To: Eva Permaul Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] bluing in tap water? When I have used tap water, I use cold running water for 5 minutes. Works fairly well, depending on the pH of your tap water. Eva Permaul wrote: > Good morning, > I was wondering if someone uses tap water to blue their slides after > Hematoxyline. If yes, do you use warm or cold water and for how long? > Thanks, > Eva > Georgetown University > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 13 Jan 2009 11:37:02 -0600 From: "Parker, Helayne" Subject: [Histonet] Spit and Diastase To: Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Content-Type: text/plain; charset="us-ascii" Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker ------------------------------ Message: 23 Date: Tue, 13 Jan 2009 12:46:15 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Processing 3-dimensional cell cultures into paraffin To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C83@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really isn't much different from processing and embedding tissue. You can trim the agar blocks to appropriate size with a scalpel (if necessary) either before or after fixation (if you trim them before fixation, do it while they are cold, so the agar is firm), and place them in a cassette just like a piece of tissue. Put them on the processor as usual, processing times the same as you would use for tissues of equivalent size, and embed as usual. One difference in sectioning however. Don't place the blocks in water after facing them off. The agar absorbs too much water and becomes soft. The blocks should be cut cold, but not moistened. Either place them on a cold plate without water, or put them in the refrigerator after facing, and take them out one at a time as you section them. ------------------------------ Message: 24 Date: Tue, 13 Jan 2009 12:49:08 -0500 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Processing 3-dimensional cell cultures into paraffin To: "Monfils, Paul" , Histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402AD8C@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Histogel works great and does not absorb water like plain agar does. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Tuesday, January 13, 2009 12:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing 3-dimensional cell cultures into paraffin I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really isn't much different from processing and embedding tissue. You can trim the agar blocks to appropriate size with a scalpel (if necessary) either before or after fixation (if you trim them before fixation, do it while they are cold, so the agar is firm), and place them in a cassette just like a piece of tissue. Put them on the processor as usual, processing times the same as you would use for tissues of equivalent size, and embed as usual. One difference in sectioning however. Don't place the blocks in water after facing them off. The agar absorbs too much water and becomes soft. The blocks should be cut cold, but not moistened. Either place them on a cold plate without water, or put them in the refrigerator after facing, and take them out one at a time as you section them. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Tue, 13 Jan 2009 12:49:13 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Spit and Diastase To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C84@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Is she doing her PAS stains right after lunch? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 15 **************************************** THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From JonSorenson <@t> chiwest.com Tue Jan 13 12:49:04 2009 From: JonSorenson <@t> chiwest.com (Sorenson, Jon (Nampa)) Date: Tue Jan 13 12:50:17 2009 Subject: [Histonet] RE: Spit and Diastase References: <20090113182042.EBF4CB7AJV@email3.catholichealth.net> Message-ID: <55F887698A32F54F8918ABC6952E0D9D010A1967@CHIMSX02.CHI.catholichealth.net> Is the person involved a smoker? We have had trouble with the saliva of people that smoke not having diastase in the past, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com ________________________________ From gayle.callis <@t> bresnan.net Tue Jan 13 12:58:25 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Jan 13 12:59:41 2009 Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval Message-ID: <000001c975b0$e9e4a720$bdadf560$@callis@bresnan.net> Neil Hand cut very thin sections, 0.5 to 1 um thin sections with a glass knife or diamond knife. The thickness very well may have made a difference for section staying on the slide during the pressure cooker retrieval he used (J Histotechnology, 21:231-236, 1998). There are publications in J Histotechnology by Nancy Troiano, et al where they microtomed PMMA embedded bone sections - I do not recall what kind of slides or adhesives they used. She has responses to Histonet questions and a search of the archives might turn up her section adhesion methods. Hopefully she is looking in to provide comments. Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT From jamie.erickson <@t> abbott.com Tue Jan 13 13:00:16 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Tue Jan 13 13:00:25 2009 Subject: [Histonet] Bone histology help Thanks..Wow Message-ID: Thank you all for the e-mails and calls about Bone histology. I was overwhelmed by the willingness of histonetters to help. My journey that is about to start into the world of bone histology will surely be easier now that I have experts to consult. Gayle Callis thanks for the information and it is good to see you still checking in on the histonet, you are a wealth of information. Thanks again.... Jamie _______________________________ Jamie Erickson Sr. Research Associate, M.S., HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From beth.millerman <@t> stiefel.com Tue Jan 13 13:22:23 2009 From: beth.millerman <@t> stiefel.com (Beth Millerman) Date: Tue Jan 13 13:23:23 2009 Subject: [Histonet] Slide writer and cassette labeler Message-ID: I am a small lab looking to purchase a cassette labeler and slide writer. Is it better to get them as separate pieces or as a combo? Hopefully, there is one that is reliable and not temperamental. Any experience and feedback is appreciated. Beth Millerman, HTL, SRA Stiefel Laboratories, Inc From Ian.Young <@t> hcmed.org Tue Jan 13 14:17:13 2009 From: Ian.Young <@t> hcmed.org (Young, Ian R) Date: Tue Jan 13 14:17:17 2009 Subject: [Histonet] GMS Contamination Message-ID: Dear Histonet, We are currently using Ventana Nexes Special Stain modules for our GMS stains. Our pathologists have been seeing bacteria all over the stains. We decontaminate our machines monthly (which is more than Ventana recommends) and make our solutions up fresh everyday. Yet the contamination persists. We did cultures on the instrument and it was found to be clear. My question is whether or not any other labs have experienced this problem and whether or not they were able to fix it. Thank you, Ian Young, HTL(ASCP) Histotechnologist Dept. of Anatomic Pathology Hennepin County Medical Center 701 Park Ave. PL Minneapolis, MN 55415 (612)873-3079 Ian.Young@hcmed.org From dont8know8me <@t> yahoo.com Tue Jan 13 14:19:42 2009 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Tue Jan 13 14:27:21 2009 Subject: [Histonet] Hematoxylin Message-ID: <526438.42438.qm@web51408.mail.re2.yahoo.com> Tanya? i've used several type of Hematoxylin in my entire life working in histology.....Different pathologist like different outcome of hematoxylin.....its hard to pleased everybody.......but i always come back w/ one commercial? Harris Hematoxylin made by Fischer......its w/? acetic acid and mercury free......try it ....letme know... ? SH26-4D From Janet.Bonner <@t> FLHOSP.ORG Tue Jan 13 14:34:44 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jan 13 14:36:03 2009 Subject: [Histonet] Spit and Diastase References: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F288F@fhosxchmb006.ADVENTISTCORP.NET> I have seen diabetics not be able to make their "diastase" work. Janet L. Bonner, HTL (ASCP) Pathology Laboratory ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Parker, Helayne Sent: Tue 1/13/2009 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Spit and Diastase Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From JMacDonald <@t> mtsac.edu Tue Jan 13 14:54:57 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 13 14:55:55 2009 Subject: [Histonet] Leica Rep Message-ID: Hi All, Trying to find out who the Leica Rep is for Southern California. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From catbo50 <@t> yahoo.com Tue Jan 13 14:55:02 2009 From: catbo50 <@t> yahoo.com (Cathy Boyd) Date: Tue Jan 13 14:56:01 2009 Subject: [Histonet] using patient blocks for positive IHC controls Message-ID: <528384.41609.qm@web112222.mail.gq1.yahoo.com> Does anyone know if there are ?any regulations on how much tissue you can cut from a patient block?? We currently use known positive patient slides for our IHC positive control slides?? One of our pathologist questioned if we could use an entire patient block ,cutting through the block for controls as long as the slide was still positive.? Or is this an individual institution decision?? She was especially concerned about CAP. From rjbuesa <@t> yahoo.com Tue Jan 13 15:16:10 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 13 15:17:11 2009 Subject: [Histonet] Spit and Diastase In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Message-ID: <168182.60463.qm@web65710.mail.ac4.yahoo.com> Spitting to do a PAS "with diastase" is repugnant and unhealthy. Also it may contaminate the sections with mouth bacteria. Use a pig diastase, that is not as "readily available as the saliva you carry around with you" but for certain more becoming. Are you going to write in your SOP that s/he who is doing this procedure is going to spit over the section? How much saliva? Before or after eating? It is just revolting and disgusting! Ren? J. --- On Tue, 1/13/09, Parker, Helayne wrote: From: Parker, Helayne Subject: [Histonet] Spit and Diastase To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 13, 2009, 12:37 PM Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Jan 13 15:47:17 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jan 13 15:48:19 2009 Subject: [Histonet] Spit and Diastase - No more expectations of expectorations In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Message-ID: My staff refused to use spit to do a DiPAS so we developed an alternative (Lasts months at 4oC - just pipette or using a dropper, add to the slides & incubate 10min at room temp). See: Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase Reagent with a Long Shelf Life for the removal of Glycogen from Tissue Sections" J. Histotechnol 25:153. No more expectations of expectorations (ie no more spitting) in the lab. Possibly the staff member expectorated on the wrong side of the slide. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Wednesday, 14 January 2009 4:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Spit and Diastase Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JonSorenson <@t> chiwest.com Tue Jan 13 15:57:28 2009 From: JonSorenson <@t> chiwest.com (Sorenson, Jon (Nampa)) Date: Tue Jan 13 16:00:35 2009 Subject: [Histonet] RE: Time & temp in oven prior to IHCs References: <20090113212951.3DC43AGDED@Email8.catholichealth.net> Message-ID: <55F887698A32F54F8918ABC6952E0D9D010A196D@CHIMSX02.CHI.catholichealth.net> 70 C for 20-30 minutes, but beware of adding an adhesive to waterbaths if you are using silane treated (plus) slides, you may be creating an adhesion problem by doing so. Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com ________________________________ From gayle.callis <@t> bresnan.net Tue Jan 13 16:10:32 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Jan 13 16:10:54 2009 Subject: [Histonet] RE: Histogel embedding mouse brain for cryotomy or vibratome In-Reply-To: References: Message-ID: <000601c975cb$c0976d10$41c64730$@callis@bresnan.net> Bob, So which type of sectioning are you doing now? Cryotomy or vibratome? And how thick do you want the sections to be? If frozen sections on snap frozen mouse brain, simply turning the temperature up to -16C or so will make sectioning much easier and get rid of the shredding, friable sections. Also, are you fixing then sucrose cryoprotecting after NBF or 4% paraformaldehyde fixation of the brain (can be either perfused or immersion fixation)? Gayle Callis HTL(ASCP)HT,MT Bozeman MT From AnthonyH <@t> chw.edu.au Tue Jan 13 16:22:47 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jan 13 16:22:58 2009 Subject: [Histonet] RE: Spit and Diastase In-Reply-To: <55F887698A32F54F8918ABC6952E0D9D010A1967@CHIMSX02.CHI.catholichealth.net> Message-ID: There is also a small co-hort of people who lack or have reduced levels of the salivary amylase enzyme (eg Nutrition Research Reviews (2003)16:37-43). Though in this case the diPAS has worked in the past. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sorenson, Jon (Nampa) Sent: Wednesday, 14 January 2009 5:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Spit and Diastase Is the person involved a smoker? We have had trouble with the saliva of people that smoke not having diastase in the past, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com ________________________________ ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Albert.Santiago <@t> uphs.upenn.edu Tue Jan 13 16:28:11 2009 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Tue Jan 13 16:28:18 2009 Subject: [Histonet] new antibody validation forms accepted by either CAP or JACHO Message-ID: Hello Histonetters, I'm looking to see if someone can suggest or provide any known CAP or JACHO "new antibody forms" that would be compliant for the inspection. Our director does not feel comfortable with the one we're presently using. Thank you Albert Santiago, HT (ASCP) Laboratory Supervisor Dermatopathology Phone 215-662-6539 Fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From bob.nienhuis <@t> gmail.com Tue Jan 13 17:06:29 2009 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Tue Jan 13 17:06:35 2009 Subject: [Histonet] RE: Histogel embedding mouse brain for cryotomy or vibratome In-Reply-To: <6707332009324210474@unknownmsgid> References: <6707332009324210474@unknownmsgid> Message-ID: <45109da50901131506n6d9dcc9wb2404fbcdd49ced6@mail.gmail.com> Currently doing Cryotomy on quickly frozen Golgi-Cox impregnated mouse brains at 100-120 microns. Cryoprotecting with 30% sucrose. Cutting at -15C. Tried fresh tissue and brief NBF perfusion(5 min). If I perfuse with NBF for 15 min, or immersion fix overnight, it seems to destroy spines and create artifact- possibly glial cells. Cutting much easier though! Might Histogel help? Bob Nienhuis UCLA / VA Medical Center On Tue, Jan 13, 2009 at 2:10 PM, gayle callis wrote: > Bob, > > So which type of sectioning are you doing now? Cryotomy or vibratome? And > how thick do you want the sections to be? If frozen sections on snap > frozen > mouse brain, simply turning the temperature up to -16C or so will make > sectioning much easier and get rid of the shredding, friable sections. > Also, are you fixing then sucrose cryoprotecting after NBF or 4% > paraformaldehyde fixation of the brain (can be either perfused or immersion > fixation)? > > Gayle Callis > HTL(ASCP)HT,MT > Bozeman MT > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jan 13 17:58:04 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jan 13 17:58:10 2009 Subject: [Histonet] Leica Rep In-Reply-To: Message-ID: Hi Jennifer The Leica rep in your area is Mike Allegro. His cell number is 713 249 8394. I will also foreward your information to Mike. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Jennifer MacDonald histonet@lists.utsouthwestern.edu Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Leica Rep 01/13/2009 02:54 PM Hi All, Trying to find out who the Leica Rep is for Southern California. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sheila_adey <@t> hotmail.com Tue Jan 13 19:50:35 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 13 19:50:39 2009 Subject: [Histonet] Full time Histo tech position in Port Huron Michigan Message-ID: We have an opening for a full time Histo tech at Port Huron Hospital in Port Huron Michigan. Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Drag n? drop?Get easy photo sharing with Windows Live? Photos. http://www.microsoft.com/windows/windowslive/photos.aspx From lpwenk <@t> sbcglobal.net Tue Jan 13 20:16:04 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jan 13 20:17:07 2009 Subject: [Histonet] Spit and Diastase In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6@mail1-schc.skaggs.net> Message-ID: <8CFBF9875BE94F529705DA50C811335D@HPPav2> Besides eating, also consider chewing of gum, or sucking on mints or candy, as a way of depleting diastase. One reason to consider using commercial amylase/diastase. Standardization. Along with lack of bacteria and epithelial cells showing up on the patient's slides. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, January 13, 2009 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Spit and Diastase Hi Gang, What would cause someone's spit to no longer work while doing a PAS w/ Diatase. She is a trainee and has done this several times prev to this w/o any issues and all the sudden her spit does not work. Did not work yesterday nor did the repeat today work. Well off to repeat it again w/ my spit. Any suggestions, Thanks Helayne Parker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Wed Jan 14 04:49:44 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Jan 14 04:50:48 2009 Subject: [Histonet] Microm STP120 and 2-propanol protocol Message-ID: Hello Histonetters, is there anyone with a Microm ST 120 processor? What protocol do you use? Even one with 2-propanol? Thanks for replying, V. Neubert From S.J.Ainsworth <@t> bsms.ac.uk Wed Jan 14 06:54:59 2009 From: S.J.Ainsworth <@t> bsms.ac.uk (S.J.Ainsworth@bsms.ac.uk) Date: Wed Jan 14 06:56:11 2009 Subject: [Histonet] hybridisation Oven Message-ID: <397EBB11B9C649428A1AB64BC16C66AA0196D9A5@EXCHANGE2.university.brighton.ac.uk> Hi I was wondering if anyone could provide some advice regarding hybridisation ovens. I were going to by a hybrisation oven from a company that said it came with both a carousel to rotate bottles/tubes or a rocking platform. They have since got back to me said it only comes with carousel. To buy one that has both a carousel and rocking platform will be much more money than I wanted to spend, so I was wondering is having rocking platform and carousel necessary? I do whole mount in situs on stage 10 chicks so they are pretty small. When I have done them in the past we used a carousel. Any suggests/thoughts would be very much appreciated. Thanks, Sophie Sophie Ainsworth Brighton and Sussex Medical school University of Sussex Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 From RCazares <@t> schosp.org Wed Jan 14 09:21:13 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Jan 14 09:22:13 2009 Subject: [Histonet] Block and Slide retention Message-ID: <0672286797B07E40AA3414F534B7CB8003F4C98B93@EXCHCCRMB.schosp.org> Hello Histonetters, I wanted to know what institutions in Illinois are doing as far as block and slide retention. I believe the guidelines that came out recently say 10 years. Our facility, as I'm sure others do, want to hold them longer. Any and all feedback will be greatly appreciated. Thanks! Ruth Cazares Histology Supervisor Department of Pathology Swedish Covenant Hospital *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From arsenn <@t> hsh.org Wed Jan 14 09:25:26 2009 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Wed Jan 14 09:26:26 2009 Subject: [Histonet] PRO Cycler BR instrument Message-ID: Hi Netters, We have a Procycler by BR Instrument & we have been calling the company with some questions and they refuse to send someone out to PM the recycler. We don't have major problems, but sometimes it doesn't want to read the temp probe, and we've had some issues that are 'solved' by shutting the machine totally off ... when we start it again, 5 minutes later, it seems to be fine. The representative we speak with wants to give us information over the phone to 'fix' the machine, but we are wondering if someone could come in that we could actually talk to and get some further PM done on it. Does anyone else use this recycler and/or have you had a tech come to service your machine? If you did, what did you bribe them with? Thank you so much for the replies to my earlier question about outside labs. WOW I got the responses to that question! Thanks again so very much! Hope everyone is having a great day... Amy HT, Holy Spirit, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From NSEARCY <@t> swmail.sw.org Wed Jan 14 09:29:49 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Jan 14 09:31:08 2009 Subject: [Histonet] Ventana Symphony Message-ID: <496DB08D.5D38.00EF.0@swmail.sw.org> Would like some feedback on any users out there. Any change in lab is difficult with some employees. That said, I think that it will actually be a plus in the lab---when the process changes are accepted. Any users out there have any comments? We are in evaluation period and have six more weeks remaining. Any instrumentation that I can have that helps with staffing shortages is a plus! thanks Nita From jqb7 <@t> cdc.gov Wed Jan 14 09:30:58 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jan 14 09:31:33 2009 Subject: [Histonet] PRO Cycler BR instrument In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402AD9D@LTA3VS011.ees.hhs.gov> We have 2 and we have service agreements on them. We have no problems getting our PMs done. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 14, 2009 10:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] PRO Cycler BR instrument Hi Netters, We have a Procycler by BR Instrument & we have been calling the company with some questions and they refuse to send someone out to PM the recycler. We don't have major problems, but sometimes it doesn't want to read the temp probe, and we've had some issues that are 'solved' by shutting the machine totally off ... when we start it again, 5 minutes later, it seems to be fine. The representative we speak with wants to give us information over the phone to 'fix' the machine, but we are wondering if someone could come in that we could actually talk to and get some further PM done on it. Does anyone else use this recycler and/or have you had a tech come to service your machine? If you did, what did you bribe them with? Thank you so much for the replies to my earlier question about outside labs. WOW I got the responses to that question! Thanks again so very much! Hope everyone is having a great day... Amy HT, Holy Spirit, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 14 10:21:23 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 14 10:22:21 2009 Subject: [Histonet] Time & temp in oven prior to IHCs ? In-Reply-To: Message-ID: <697497.65267.qm@web65707.mail.ac4.yahoo.com> Your drying time seems correct so you should look into other possible causes, like the infiltration or processing times. Ren? J. --- On Tue, 1/13/09, sheila adey wrote: From: sheila adey Subject: [Histonet] Time & temp in oven prior to IHCs ? To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 13, 2009, 1:17 PM Good afternoon Netters, Our prostate core biopsies have been "floating" off during the IHCs. We are wondering how long other institutions are drying the slides prior to going to water? We typically dry for 25 min at 65 degrees and we use charged slides. These slides are retrieved in the microwave. Thanks in advance for your input. :)Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 14 10:23:10 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 14 10:24:09 2009 Subject: [Histonet] Leica Rep In-Reply-To: Message-ID: <70151.16541.qm@web65711.mail.ac4.yahoo.com> Contact Leica microsystems! Ren? J. --- On Tue, 1/13/09, Jennifer MacDonald wrote: From: Jennifer MacDonald Subject: [Histonet] Leica Rep To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 13, 2009, 3:54 PM Hi All, Trying to find out who the Leica Rep is for Southern California. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 14 10:25:18 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 14 10:25:26 2009 Subject: [Histonet] using patient blocks for positive IHC controls In-Reply-To: <528384.41609.qm@web112222.mail.gq1.yahoo.com> Message-ID: <568412.30535.qm@web65703.mail.ac4.yahoo.com> This is mostly an individual institution decision and if the block is from a case old enough as to be in the list to dispose off, there cannot be any restrictions because it is going to be disposed off anyway. Ren? J. --- On Tue, 1/13/09, Cathy Boyd wrote: From: Cathy Boyd Subject: [Histonet] using patient blocks for positive IHC controls To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 13, 2009, 3:55 PM Does anyone know if there are ?any regulations on how much tissue you can cut from a patient block?? We currently use known positive patient slides for our IHC positive control slides?? One of our pathologist questioned if we could use an entire patient block ,cutting through the block for controls as long as the slide was still positive.? Or is this an individual institution decision?? She was especially concerned about CAP. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shakun.Aswani <@t> acologix.com Wed Jan 14 10:54:33 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Wed Jan 14 10:54:38 2009 Subject: [Histonet] Microm STP120 and 2-propanol protocol In-Reply-To: References: Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301A842DF@EXCHANGE.acologix.com> Hi Neubert, I have Microm ST 120 processor. What are you processing? Curtis D. King from that company is very good in histology contact him # 1-800-522-7270 x672 His email: cking@rallansci.com Shakun -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V. Neubert Sent: Wednesday, January 14, 2009 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microm STP120 and 2-propanol protocol Hello Histonetters, is there anyone with a Microm ST 120 processor? What protocol do you use? Even one with 2-propanol? Thanks for replying, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Jan 14 11:01:52 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jan 14 11:02:01 2009 Subject: [Histonet] Re: Refurbished Equipment Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23746@PHSXMB30.partners.org> To all: I sent a recent request to the Histonet asking for people's views re: Leica's ST5020 stainer and CV5030 coverslipper. Many people replied enthusiastically about these instruments. Thank you very much for your input. I had several replies from companies who sell refurbished equipment. My question: Does anyone have the earlier model stainer (Leica XL) and how does it differ from the newer model (i.e. was anything-important-improved upon)? Obviously, the price difference is great. Money may be an issue, so if we can go the less expensive route, so much better. We have never purchased refurbished equipment, so would be interested in your ideas in general as well. Thanks again! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From JFerrer <@t> lifecell.com Wed Jan 14 11:09:44 2009 From: JFerrer <@t> lifecell.com (Ferrer, Joselito) Date: Wed Jan 14 11:10:49 2009 Subject: [Histonet] nuetralizing DAB Message-ID: <26263B1C4C33EE4292EB294531ABB0A486486E2907@LC-HQEXCH01.lifecell.biz> Good Afternoon everyone, Is there any adverse affect if combining hydrogen peroxide and DAB when disposing? What is the proper way to dispose of stable DAB? Thank you for your reply Joselito Ferrer *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From ejschmid <@t> ucalgary.ca Wed Jan 14 11:29:57 2009 From: ejschmid <@t> ucalgary.ca (ejschmid@ucalgary.ca) Date: Wed Jan 14 11:30:25 2009 Subject: [Histonet] GMA, stains, and autofluorescence Message-ID: <62821.136.159.74.34.1231954197.squirrel@136.159.74.34> Hi, I using a procedure whereby I need the GMA resin of a thin section to fluoresce under rhodamine or UV but under other wavelengths. Currently I am staining the plastic with Eosin. Unfortunately, this causes the plastic to fluoresce under both rhodamine and FITC, something I'm trying to avoid. Could anyone suggest an alternative stain? thanks! Eric From ejschmid <@t> ucalgary.ca Wed Jan 14 11:29:24 2009 From: ejschmid <@t> ucalgary.ca (ejschmid@ucalgary.ca) Date: Wed Jan 14 11:30:39 2009 Subject: [Histonet] GMA, stains, and autofluorescence Message-ID: <62818.136.159.74.34.1231954164.squirrel@136.159.74.34> Hi, I using a procedure whereby I need the GMA resin of a thin section to fluoresce under rhodamine or UV but under other wavelengths. Currently I am staining the plastic with Eosin. Unfortunately, this causes the plastic to fluoresce under both rhodamine and FITC, something I'm trying to avoid. Could anyone suggest an alternative stain? thanks! Eric From sjromey <@t> bellsouth.net Wed Jan 14 11:50:01 2009 From: sjromey <@t> bellsouth.net (sjromey@bellsouth.net) Date: Wed Jan 14 11:50:06 2009 Subject: [Histonet] Refurbished equipment Message-ID: <011420091750.22860.496E25C70002D31A0000594C22218675169B0A02D2089B9A019C04040A0DBF970A03019D069C@att.net> Hi. I am also interested in the Refurbished Equipment. Looking for a H & E slide stainer. Is buying a refurbished stainer like buying many used cars...buying someone else's problems??? thanks sjromey@bellsouth.net From Charlotte.Kopczynski <@t> baycare.org Wed Jan 14 12:09:11 2009 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Wed Jan 14 12:09:17 2009 Subject: [Histonet] RE: Histonet Digest, Vol 62, Issue 18 In-Reply-To: Message-ID: We have a Leica XL stainer with transfer station for sale. It was purchased in 2006 and only used one year. Due to consolidation of histology processing, we do not have room for this stainer. Make me an offer... Thanks, Charlotte Kopczynski, HTL (ASCP) Pathology Manager Morton Plant Mease Health System BayCare Health System 727-461-8246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, January 14, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 62, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Refurbished equipment (sjromey@bellsouth.net) ---------------------------------------------------------------------- Message: 1 Date: Wed, 14 Jan 2009 17:50:01 +0000 From: sjromey@bellsouth.net Subject: [Histonet] Refurbished equipment To: Histonet@lists.utsouthwestern.edu Message-ID: <011420091750.22860.496E25C70002D31A0000594C22218675169B0A02D2089B9A019C 04040A0DBF970A03019D069C@att.net> Content-Type: text/plain; charset="utf-8" Hi. I am also interested in the Refurbished Equipment. Looking for a H & E slide stainer. Is buying a refurbished stainer like buying many used cars...buying someone else's problems??? thanks sjromey@bellsouth.net ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 18 **************************************** Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From kmerriam2003 <@t> yahoo.com Wed Jan 14 12:22:55 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jan 14 12:23:52 2009 Subject: [Histonet] Complement C3 for mouse FFPE Message-ID: <166234.88774.qm@web50309.mail.re2.yahoo.com> Hi, ? Can anyone recommend a good antibody for complement C3 on mouse FFPE tissue? ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From anh2006 <@t> med.cornell.edu Wed Jan 14 12:38:40 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Jan 14 12:38:49 2009 Subject: [Histonet] murine BM monocytes Message-ID: Is anyone doing F4/80 on frozen mouse bone sections? How about other monocyte markers? Would love to hear about any experiences. Thanks, Andrea -- From TraceyG <@t> adhb.govt.nz Wed Jan 14 13:30:04 2009 From: TraceyG <@t> adhb.govt.nz (Tracey Gunn) Date: Wed Jan 14 13:31:06 2009 Subject: [Histonet] Re: Spit and Diastase Message-ID: Hi Histonetters I am one of the people in the world with deficient salivary diastase. I have been working in Histology labs for 15 years and initially had OK diastase, but over the past 4 years my saliva doesn't digest the glycogen. I do not smoke, don't have diabetes, don't chew gum or eat anything before doing DPAS!! Tracey Gunn Section Leader - Histology LabPlus Auckland City Hospital (09) 307 4949 ext 6121 From bill.mcmanus <@t> usu.edu Wed Jan 14 13:51:46 2009 From: bill.mcmanus <@t> usu.edu (Bill McManus) Date: Wed Jan 14 13:53:12 2009 Subject: [Histonet] blocking aldehydes for staining References: <4F708ED4-9FC8-49BF-B0A4-767644ACFFAF@usu.edu> Message-ID: <48461FF8-D55B-4569-A7B7-DC5B3D65CA21@usu.edu> > > > We are trying to use Congo Red to stain starch for confocal, but it > is staining everything. The sample is condensed milk and very soft > without fixation. Does anyone know of a good method to block the > glutaraldehyde so that the Congo Red will only stain the starch? > > Bill McManus > Western Dairy Center > Utah State University > bill.mcmanus@usu.edu > From eca9 <@t> georgetown.edu Wed Jan 14 13:55:48 2009 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Wed Jan 14 13:55:52 2009 Subject: [Histonet] staining rat tissue with monoclonal antibody? Message-ID: <496E4344.1080001@georgetown.edu> Good afternoon. I just had someone ask me about staining rat tissue using an monoclonal (mouse) antibody. I have never done this before and so was wondering if there is anything in particular I need to keep in mind. Does mouse antibodies cause a problem when staining rat tissues? Do I have to use a specific kit or specific secondary antibody? Can I use a biotinylated goat-anti-mouse secondary followed by ABC? Thanks for your help, Eva From bill.mcmanus <@t> usu.edu Wed Jan 14 14:14:53 2009 From: bill.mcmanus <@t> usu.edu (Bill McManus) Date: Wed Jan 14 14:14:57 2009 Subject: [Histonet] blocking aldehydes for staining In-Reply-To: <18622.84070.qm@web1101.biz.mail.sk1.yahoo.com> References: <4F708ED4-9FC8-49BF-B0A4-767644ACFFAF@usu.edu> <48461FF8-D55B-4569-A7B7-DC5B3D65CA21@usu.edu> <18622.84070.qm@web1101.biz.mail.sk1.yahoo.com> Message-ID: <0B305D19-112B-4F52-AA4D-2CDAE0D158BC@usu.edu> Paula: Do you know of any stains that fluoresce and stain starches? On Jan 14, 2009, at 1:11 PM, Paula Pierce wrote: > Congo Red is for amyloid. > > I suggest you use an iodine solution to stain starch. It will stain > it black. > > From: Bill McManus > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, January 14, 2009 1:51:46 PM > Subject: [Histonet] blocking aldehydes for staining > > > > > > > > We are trying to use Congo Red to stain starch for confocal, but it > > is staining everything. The sample is condensed milk and very > soft > > without fixation. Does anyone know of a good method to block the > > glutaraldehyde so that the Congo Red will only stain the starch? > > > > > > Bill McManus > > Western Dairy Center > > Utah State University > > bill.mcmanus@usu.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Bill McManus Western Dairy Center Utah State University bill.mcmanus@usu.edu From sjchtascp <@t> yahoo.com Wed Jan 14 14:53:07 2009 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Jan 14 14:53:11 2009 Subject: [Histonet] Work flow, quality issues Message-ID: <174479.25186.qm@web38201.mail.mud.yahoo.com> I have worked in several HT labs and as expected most differ in individual technique.? Most are very good as far as work flow from grossing, processing, embedding and sectioning.? I work in a lab now where the grossing and processing of "like specimens" are in case order until there embedded.? Embedded totally out of order, even within a case.? One person will rough cut the blocks on 1 microtome, approx 20 microns.? After all the embedding is done the? trimmed? blocks are put in order, placed? on ice in which about half are to be cut on another microtome.? Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns,? 5 microns at a time, to get a complete section.? This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with.? Often I have to re-trim the blocks to match my microtome then rehydrate them again.? This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome.? At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming.? Then I rehydrate and ice the tissue..?? With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality.? Now that I'm back nothing has changed.? The pathologist still has section quality issues.? What ever happen to the idea of Quality Improvement.? The works getting done I suppose, maybe thats all that matters these days. From pritchm <@t> ccf.org Wed Jan 14 15:09:46 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Wed Jan 14 15:09:57 2009 Subject: [Histonet] staining rat tissue with monoclonal antibody? In-Reply-To: <496E4344.1080001@georgetown.edu> Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E0215131A@CCHSCLEXMB56.cc.ad.cchs.net> Hi: We routinely perform immunohistochemistry (IHC) to detect antigens on rat tissues. A mouse mAb should be fine for use in your rat samples. You will not need a special kit, just an anti-mouse secondary Ab. One runs into trouble when they use a mouse (or rat) primary on mouse (or rat) tissue, respectively. Then there are kits (i.e. Mouse-on-Mouse kits) that can decrease background staining due to the anti-mouse IgG secondary you would need to use to detect the primary Ab. From my limited knowledge of IHC, I would say that you could certainly use a biotinylated goat-anti-mouse secondary followed by ABC. I do this all the time and use DAB for visualization of my antigen of interest. Cheers: ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, January 14, 2009 2:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining rat tissue with monoclonal antibody? Good afternoon. I just had someone ask me about staining rat tissue using an monoclonal (mouse) antibody. I have never done this before and so was wondering if there is anything in particular I need to keep in mind. Does mouse antibodies cause a problem when staining rat tissues? Do I have to use a specific kit or specific secondary antibody? Can I use a biotinylated goat-anti-mouse secondary followed by ABC? Thanks for your help, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From anh2006 <@t> med.cornell.edu Wed Jan 14 15:11:12 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Jan 14 15:12:17 2009 Subject: [Histonet] staining rat tissue with monoclonal antibody? In-Reply-To: <496E4344.1080001@georgetown.edu> References: <496E4344.1080001@georgetown.edu> Message-ID: Just make sure you use an anti-mouse IgG antibody that is not cross reactive to rat IgG. Jackson ImmunoResearch is the best place to find these reagents ... I suggest the donkey as host, it works best for me, least background. Beautiful results ... >Good afternoon. >I just had someone ask me about staining rat tissue using an >monoclonal (mouse) antibody. I have never done this before and so >was wondering if there is anything in particular I need to keep in >mind. Does mouse antibodies cause a problem when staining rat >tissues? Do I have to use a specific kit or specific secondary >antibody? Can I use a biotinylated goat-anti-mouse secondary >followed by ABC? >Thanks for your help, >Eva -- From pritchm <@t> ccf.org Wed Jan 14 15:22:33 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Wed Jan 14 15:23:41 2009 Subject: [Histonet] Complement C3 for mouse FFPE In-Reply-To: <166234.88774.qm@web50309.mail.re2.yahoo.com> Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E0215131B@CCHSCLEXMB56.cc.ad.cchs.net> Kim: When you say 'complement C3' do you mean cleavage products of C3 after C3 activation (C3b-iC3b/C3c) or C3 iteself? C3b-iC3b/C3c are covalently attached to the surface of cells/pathogens, so I suspect this is what you would like to localize in your mouse tissue. We use an antibody in frozen sections that works beautifully (Cell Sciences clone 2/11). We have only limited experience with this clone in FFPE tissues. It likely requires antigen retrieval; thus far, we have tried using proteinase K and that was not successful. Because the frozen sections look so nice, we have not tried to localize C3b-iC3b/C3c in FFPE lately. If I had it to try again, I would start with citrate buffer antigen retrieval in my microwave pressure cooker. Hope this helps. ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, January 14, 2009 1:23 PM To: Histonet Subject: [Histonet] Complement C3 for mouse FFPE Hi, ? Can anyone recommend a good antibody for complement C3 on mouse FFPE tissue? ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From david.kinsley <@t> spcorp.com Wed Jan 14 15:32:36 2009 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Wed Jan 14 15:33:58 2009 Subject: [Histonet] Autoradiography troubleshooting urgent Message-ID: Hi, I have an urgent question concerning trouble shooting of emulsion dipped histology slides. After developing emulsion dipped in situ slides stored in refrigerator for 4 weeks I found that all my slides have a cracked window appearance throughout the slide. This effect is clearly due to the emulsion because it could be eliminated when the emulsion was wiped out from both sides of the slide. Can anyone help me to figure out what has caused this effect. It has never happened to my in situ slides before and I have been following the same protocol for years. I'm using all Kodak reagents, D-19 developer, Fixer, and NTB emulsion. Any advice is appreciated and I need them quick since I still have another batch of slides to develop this week. Thanks Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From b-frederick <@t> northwestern.edu Wed Jan 14 15:42:43 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Jan 14 15:42:56 2009 Subject: [Histonet] staining rat tissue with monoclonal antibody? In-Reply-To: <496E4344.1080001@georgetown.edu> Message-ID: <000001c97691$0b72faf0$d00f7ca5@lurie.northwestern.edu> We try and find a mouse anti-rat antibody if we can and run rat secondary (Dako makes one) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, January 14, 2009 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining rat tissue with monoclonal antibody? Good afternoon. I just had someone ask me about staining rat tissue using an monoclonal (mouse) antibody. I have never done this before and so was wondering if there is anything in particular I need to keep in mind. Does mouse antibodies cause a problem when staining rat tissues? Do I have to use a specific kit or specific secondary antibody? Can I use a biotinylated goat-anti-mouse secondary followed by ABC? Thanks for your help, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Jan 14 16:08:18 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Jan 14 16:08:31 2009 Subject: [Histonet] blocking aldehydes for staining References: <4F708ED4-9FC8-49BF-B0A4-767644ACFFAF@usu.edu> <48461FF8-D55B-4569-A7B7-DC5B3D65CA21@usu.edu> <18622.84070.qm@web1101.biz.mail.sk1.yahoo.com> <0B305D19-112B-4F52-AA4D-2CDAE0D158BC@usu.edu> Message-ID: Use an aldehyde block on the slides (http://stainsfile.info/StainsFile/stain/schiff/reaction-block.htm) as glutaraldehyde often has an active aldehyde group ready to react, then try a PAS using a Schiff's reagent made with acriflavine, proflavine or acridine orange (http://stainsfile.info/StainsFile/stain/schiff/pafs.htm and http://stainsfile.info/StainsFile/stain/schiff/pas-standard.htm). After 10 minutes in the Schiff's reagent after step 7, put the section in 1% acid alcohol for 5 minutes or so, then wash as in step 8 and carry on. Bryan Llewellyn ----- Original Message ----- From: "Bill McManus" To: "Paula Pierce" ; Sent: Wednesday, January 14, 2009 12:14 PM Subject: Re: [Histonet] blocking aldehydes for staining > Paula: > > Do you know of any stains that fluoresce and stain starches? > > On Jan 14, 2009, at 1:11 PM, Paula Pierce wrote: > >> Congo Red is for amyloid. >> >> I suggest you use an iodine solution to stain starch. It will stain it >> black. >> >> From: Bill McManus >> To: histonet@lists.utsouthwestern.edu >> Sent: Wednesday, January 14, 2009 1:51:46 PM >> Subject: [Histonet] blocking aldehydes for staining >> >> >> > >> > >> > We are trying to use Congo Red to stain starch for confocal, but it >> > is staining everything. The sample is condensed milk and very >> soft >> > without fixation. Does anyone know of a good method to block the >> > glutaraldehyde so that the Congo Red will only stain the starch? >> >> >> > >> > Bill McManus >> > Western Dairy Center >> > Utah State University >> > bill.mcmanus@usu.edu >> > >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Bill McManus > Western Dairy Center > Utah State University > bill.mcmanus@usu.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Jan 14 19:20:38 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jan 14 19:20:41 2009 Subject: [Histonet] Spit and Diastase Message-ID: <582736990901141720o6c5720f0nc1beb081e4d5999@mail.gmail.com> Yeah, Spitting on a slide is groody ... get a pig to do it instead hehe! Amos Message: 4 Date: Tue, 13 Jan 2009 13:16:10 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Spit and Diastase To: histonet@lists.utsouthwestern.edu, "Parker, Helayne" Message-ID: <168182.60463.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Spitting to do a PAS "with diastase" is repugnant and unhealthy. Also it may contaminate the sections with mouth bacteria. Use a pig diastase, that is not as "readily available as the saliva you carry around with you" but for certain more becoming. Are you going to write in your SOP that s/he who is doing this procedure is going to spit over the section? How much saliva? Before or after eating? It is just revolting and disgusting! Ren? J. From histocola <@t> yahoo.com Thu Jan 15 07:04:32 2009 From: histocola <@t> yahoo.com (Kathy Lambeth) Date: Thu Jan 15 07:05:34 2009 Subject: [Histonet] Wilder's Reticulum stain Message-ID: <162168.25536.qm@web90406.mail.mud.yahoo.com> --- On Thu, 1/15/09, Kathy Lambeth wrote: From: Kathy Lambeth Subject: Wilder's Reticulum stain To: histonet@list.utsouthwestern.edu Date: Thursday, January 15, 2009, 6:25 AM I just relocated to a new lab and they use Wilder's Retic stain.? They have been unable to get uranium nitrate.? Is there a substitute; another supplier; or different technic they should use.? Any suggestions are welcome. ? Kathy ? From NHeath <@t> Lifespan.org Thu Jan 15 07:24:32 2009 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Jan 15 07:25:38 2009 Subject: [Histonet] Type I and TYpe II Immunos for fiber typing Message-ID: <130E8991F210424096EFC6F42EA33B2403EADD52@LSCOEXCH1.lsmaster.lifespan.org> Hi Everyone :) Does anyone do or know of antibodies for immuno staining of muscle for fiber typing?? If you do catalog numbers and company that you get the antibodies from would be greatly appreciated and also your procedure would also be nice :) Neuropath Nancy @ RIH From jamie.erickson <@t> abbott.com Thu Jan 15 09:28:03 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu Jan 15 09:29:10 2009 Subject: [Histonet] Question about Mercedes Medical star frost slides, comments? Message-ID: Hi All, I was wondering if anyone out there has used Mercedes medical starfrost adhesive slides and how they hold up in IHC. MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235. I am thinking about changing to these slides from our VWR superfrost plus slides to save money but I would like to hear how they hold up in IHC Ag retrieval etc... I have samples from them and will try them on some IHC but I thought I'd get someone else's thoughts. If you have an opinion on the quality of the slides please let me know. Thanks, Jamie _______________________________ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From hardin <@t> oncology.wisc.edu Thu Jan 15 09:29:04 2009 From: hardin <@t> oncology.wisc.edu (Joe Hardin) Date: Thu Jan 15 09:30:09 2009 Subject: [Histonet] prion contaminated tissue processing Message-ID: <496F5640.7010302@oncology.wisc.edu> Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? From akbitting <@t> geisinger.edu Thu Jan 15 09:29:19 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Jan 15 09:30:25 2009 Subject: [Histonet] PA job Message-ID: <496F0FFF.2B7F.00C9.0@geisinger.edu> Geisinger Medical Center in Danville, PA is searching for two Histotechnologists. We have a dayshift 0.6 tech position and a nightshift 8P-4A FT position available. Visit our website at www.geisinger.org to see more information about our Health System. Contact me for more information at 570-214-9634 or send me an email. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From jqb7 <@t> cdc.gov Thu Jan 15 09:31:37 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jan 15 09:31:52 2009 Subject: [Histonet] prion contaminated tissue processing In-Reply-To: <496F5640.7010302@oncology.wisc.edu> References: <496F5640.7010302@oncology.wisc.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402ADAC@LTA3VS011.ees.hhs.gov> It will not. Check out the CDC and/or the WHO guidelines. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Thursday, January 15, 2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prion contaminated tissue processing Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? From cmiller <@t> physlab.com Thu Jan 15 09:35:50 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Jan 15 09:35:51 2009 Subject: [Histonet] Work flow, quality issues In-Reply-To: <174479.25186.qm@web38201.mail.mud.yahoo.com> References: <174479.25186.qm@web38201.mail.mud.yahoo.com> Message-ID: <005701c97726$f2169a80$4402a8c0@plab.local> What is the gain??? I am confused by their methods. I also face(rough cut) and section on the same microtome. I noticed over the years that cuts on the same microtome and block, one tech can get a thin section just by the way they turn the flywheel. A heavy handed cutter can make the section thicker on the same instrument and block. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Wednesday, January 14, 2009 2:53 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Work flow, quality issues I have worked in several HT labs and as expected most differ in individual technique.? Most are very good as far as work flow from grossing, processing, embedding and sectioning.? I work in a lab now where the grossing and processing of "like specimens" are in case order until there embedded.? Embedded totally out of order, even within a case.? One person will rough cut the blocks on 1 microtome, approx 20 microns.? After all the embedding is done the? trimmed? blocks are put in order, placed? on ice in which about half are to be cut on another microtome.? Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns,? 5 microns at a time, to get a complete section.? This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with.? Often I have to re-trim the blocks to match my microtome then rehydrate them again.? This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome.? At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming.? Then I rehydrate and ice the tissue..?? With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality.? Now that I'm back nothing has changed.? The pathologist still has section quality issues.? What ever happen to the idea of Quality Improvement.? The works getting done I suppose, maybe thats all that matters these days. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From JWeems <@t> sjha.org Thu Jan 15 09:39:10 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 15 09:39:23 2009 Subject: [Histonet] Question about Mercedes Medical star frost slides, comments? In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5299AFA@ITSSSXM01V6.one.ads.che.org> We use their cheaper ones - CAS 9308 W - and they work just fine. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jamie E Erickson Sent: Thursday, January 15, 2009 10:28 AM To: histonet histonet Subject: [Histonet] Question about Mercedes Medical star frost slides,comments? Hi All, I was wondering if anyone out there has used Mercedes medical starfrost adhesive slides and how they hold up in IHC. MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235. I am thinking about changing to these slides from our VWR superfrost plus slides to save money but I would like to hear how they hold up in IHC Ag retrieval etc... I have samples from them and will try them on some IHC but I thought I'd get someone else's thoughts. If you have an opinion on the quality of the slides please let me know. Thanks, Jamie _______________________________ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From tp2 <@t> medicine.wisc.edu Thu Jan 15 09:52:28 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu Jan 15 09:52:45 2009 Subject: [Histonet] prion contaminated tissue processing Message-ID: <496F075C020000DF000147D5@gwmail.medicine.wisc.edu> Joe, You might want to get a hold of somebody over at the WVDL. I know that when I worked there, we wore gloves when sectioning any blocks that may have come from deer with CWD ie were potentially contaminated with prion. Tom Pier >>> Joe Hardin 01/15/09 9:32 AM >>> Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? From shive003 <@t> umn.edu Thu Jan 15 10:48:07 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 15 10:48:12 2009 Subject: [Histonet] prion contaminated tissue processing References: <496F5640.7010302@oncology.wisc.edu> Message-ID: <5C392659A0D44D9486BD17058548476E@auxs.umn.edu> Joe, I do IHC for prion diseases in animals. No, tissue processing will NOT render prion-infected tissue safe for sectioning under normal conditions. Incubating in formic acid won't even totally inactivate the abnormal protein. We have to use a dedicated microtome in a dedicated room, wear nitrile gloves and disposable lab coats and booties, and stain the slides on dedicated autostainers. As others have said, check out the CDC website for more information. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Joe Hardin" To: Sent: Thursday, January 15, 2009 9:29 AM Subject: [Histonet] prion contaminated tissue processing > Hi All, > Does anyone know if tissue processing for paraffin embedding will render > prion infected tissue safe for sectioning? > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Thu Jan 15 11:15:50 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 15 11:15:58 2009 Subject: [Histonet] Work flow, quality issues References: <174479.25186.qm@web38201.mail.mud.yahoo.com> Message-ID: <07905661C028439FA30A5A399871F6B7@auxs.umn.edu> I have my techs be the only person who handles a block from facing off to finished microtomy. They put their initials on the slide label, so if that block needs recuts in the following days, the same tech and microtome will be used. That way, the least amount of tissue is lost off the block face. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Steven Coakley" To: Sent: Wednesday, January 14, 2009 2:53 PM Subject: [Histonet] Work flow, quality issues I have worked in several HT labs and as expected most differ in individual technique. Most are very good as far as work flow from grossing, processing, embedding and sectioning. I work in a lab now where the grossing and processing of "like specimens" are in case order until there embedded. Embedded totally out of order, even within a case. One person will rough cut the blocks on 1 microtome, approx 20 microns. After all the embedding is done the trimmed blocks are put in order, placed on ice in which about half are to be cut on another microtome. Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns, 5 microns at a time, to get a complete section. This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with. Often I have to re-trim the blocks to match my microtome then rehydrate them again. This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome. At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming. Then I rehydrate and ice the tissue.. With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality. Now that I'm back nothing has changed. The pathologist still has section quality issues. What ever happen to the idea of Quality Improvement. The works getting done I suppose, maybe thats all that matters these days. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Thu Jan 15 11:23:19 2009 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Jan 15 11:23:26 2009 Subject: [Histonet] Question about Mercedes Medical star frost slides, comments? Message-ID: <161432.73344.qm@web58604.mail.re3.yahoo.com> I made the switch to Mercedes a while back. I use their clipped corner slides (MER7200) because they work great on our slide printer. I also use their Plus Starfrost slides (MER7255)?for?our IHC. The?sections stay on?great,?even through?antigen retrieval. Probably the best plus slide?I have used. Try their samples, I'm sure you will be pleased.? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Thu, 1/15/09, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Question about Mercedes Medical star frost slides, comments? To: "Jamie E Erickson" , "histonet histonet" Date: Thursday, January 15, 2009, 10:39 AM We use their cheaper ones - CAS 9308 W - and they work just fine. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jamie E Erickson Sent: Thursday, January 15, 2009 10:28 AM To: histonet histonet Subject: [Histonet] Question about Mercedes Medical star frost slides,comments? Hi All, ? ? ? ? ? ? ???I was wondering if anyone out there has used Mercedes medical starfrost adhesive slides and how they hold up in IHC. MER 7255 - Slides, adhesive, 90, Starfrost, 10 gross - $235. I am thinking about changing to these slides from our VWR superfrost plus slides to save money but I would like to hear how they hold up in IHC Ag retrieval etc... I have samples from them and will try them on some IHC but I thought I'd get someone else's thoughts.? If you have an opinion on the quality of the slides please let me know. Thanks, Jamie _______________________________ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 15 11:29:16 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 15 11:29:19 2009 Subject: [Histonet] Work flow, quality issues In-Reply-To: <174479.25186.qm@web38201.mail.mud.yahoo.com> Message-ID: <399174.47147.qm@web65716.mail.ac4.yahoo.com> Steven: Are you just complaining to vent some sort of frustration, or can you actually do something about it? If venting, my sympathies go to you. What you describe could become the nightmare of anybody with the slightest idea about histology good practices, not to mention somebody that tries to take the flow into a "more or less" Lean semblance. The "muda" is astonishing. ? If you can do something about, what you describe you are used to do is just what has to be done. Use your knowledge and credentials to try to solve the mess you describe. You know how to do it and good luck. I am sure that "somebody" in that lab has done it that way for years and will be difficult to made to change. Ren? J. --- On Wed, 1/14/09, Steven Coakley wrote: From: Steven Coakley Subject: [Histonet] Work flow, quality issues To: Histonet@lists.utsouthwestern.edu Date: Wednesday, January 14, 2009, 3:53 PM I have worked in several HT labs and as expected most differ in individual technique.? Most are very good as far as work flow from grossing, processing, embedding and sectioning.? I work in a lab now where the grossing and processing of "like specimens" are in case order until there embedded.? Embedded totally out of order, even within a case.? One person will rough cut the blocks on 1 microtome, approx 20 microns.? After all the embedding is done the? trimmed? blocks are put in order, placed? on ice in which about half are to be cut on another microtome.? Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns,? 5 microns at a time, to get a complete section.? This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with.? Often I have to re-trim the blocks to match my microtome then rehydrate them again.? This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimmed my own blocks for a specific microtome.? At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming.? Then I rehydrate and ice the tissue..?? With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality.? Now that I'm back nothing has changed.? The pathologist still has section quality issues.? What ever happen to the idea of Quality Improvement.? The works getting done I suppose, maybe thats all that matters these days. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 15 11:31:48 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 15 11:31:51 2009 Subject: [Histonet] Wilder's Reticulum stain In-Reply-To: <162168.25536.qm@web90406.mail.mud.yahoo.com> Message-ID: <738857.98554.qm@web65715.mail.ac4.yahoo.com> Try phosphotungstic acid at the same concentration. Ren? J. --- On Thu, 1/15/09, Kathy Lambeth wrote: From: Kathy Lambeth Subject: [Histonet] Wilder's Reticulum stain To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 8:04 AM --- On Thu, 1/15/09, Kathy Lambeth wrote: From: Kathy Lambeth Subject: Wilder's Reticulum stain To: histonet@list.utsouthwestern.edu Date: Thursday, January 15, 2009, 6:25 AM I just relocated to a new lab and they use Wilder's Retic stain.? They have been unable to get uranium nitrate.? Is there a substitute; another supplier; or different technic they should use.? Any suggestions are welcome. ? Kathy ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 15 11:32:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 15 11:33:41 2009 Subject: [Histonet] prion contaminated tissue processing In-Reply-To: <496F5640.7010302@oncology.wisc.edu> Message-ID: <739925.22467.qm@web65707.mail.ac4.yahoo.com> NO, it will not. Ren? J. --- On Thu, 1/15/09, Joe Hardin wrote: From: Joe Hardin Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beth.Fye <@t> HCAhealthcare.com Thu Jan 15 11:34:43 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Thu Jan 15 11:35:04 2009 Subject: [Histonet] New Dako IHC Instrument and T.P.I.D. Message-ID: <938F8EC5A524D34EB5796E23E52781D3039D3A435E@NADCWPMSGCMS05.hca.corpad.net> I'm curious if anyone out there has any feedback or comments on the new Dako IHC Instrument, Autostainer Link 48 and/or their TPID (True Positive ID). We are in desparate need of replacing our current DAKO instruments, and are probably going to stick with DAKO again after looking at several other instruments. I just thought I check to see if anyone using it has anything to say. Beth From Jackie.O'Connor <@t> abbott.com Thu Jan 15 11:39:50 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jan 15 11:40:13 2009 Subject: [Histonet] prion contaminated tissue processing In-Reply-To: <739925.22467.qm@web65707.mail.ac4.yahoo.com> Message-ID: Viable prions have been found in >20 year old paraffin blocks. Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbuesa@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. Ren? J. --- On Thu, 1/15/09, Joe Hardin wrote: From: Joe Hardin Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Jan 15 11:46:19 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jan 15 11:46:41 2009 Subject: [Histonet] prion contaminated tissue processing In-Reply-To: References: <739925.22467.qm@web65707.mail.ac4.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402ADB2@LTA3VS011.ees.hhs.gov> Please read the protocols at the CDC and/or the WHO website. Bleach in not effective. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, January 15, 2009 12:40 PM To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] prion contaminated tissue processing Viable prions have been found in >20 year old paraffin blocks. Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbuesa@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. Ren? J. --- On Thu, 1/15/09, Joe Hardin wrote: From: Joe Hardin Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dnannenga <@t> incytepathology.com Thu Jan 15 12:02:45 2009 From: dnannenga <@t> incytepathology.com (Debra D. Nannenga) Date: Thu Jan 15 12:02:50 2009 Subject: [Histonet] paraffin Message-ID: <706224670091FE47997AEF88EFADE7CAB25A01@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello Histonetters: We are currently using Surgipath's Formula "R" paraffin. They are now charging us a handling fee for each container. I have tried looking at different formulas at different companies trying to find a comparable formula. It seems that no one will list the polymer combinations or ingredients. I was wondering if anyone else had encountered this problem? Does anyone know of a paraffin type that is similar to or the same as the Formula "R"? I would appreciate any feedback that folks are willing to share. Thanks, Debbie Debbie Nannenga, HTL(ASCP),QIHC InCyte Pathology Spokane Valley, Washington From rfields <@t> gidocs.net Thu Jan 15 12:34:26 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Jan 15 12:36:27 2009 Subject: [Histonet] prion contaminated tissue processing Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F6F949A@GIEXCHANGE.gidocs.net> An article entitled "Safe Handling of Transmissible Spongiform Encephalopathies Specimens in the Histopathology Laboratory" written by Konnie Zeitner was published in the JOH June 2007.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, January 15, 2009 11:40 AM To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] prion contaminated tissue processing Viable prions have been found in >20 year old paraffin blocks. Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbuesa@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. Ren? J. --- On Thu, 1/15/09, Joe Hardin wrote: From: Joe Hardin Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Thu Jan 15 13:28:17 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 15 13:29:21 2009 Subject: [Histonet] RE: Prion Contamination In-Reply-To: <01ee71c200003698@HolyRedeemer.com> Message-ID: Not only will processing NOT render it safe, but your cut, stained, and mounted slides will still be infectious UNLESS the procedure to inactivate using formic acid is followed before fixation and processing. If the tissue is fixed (formalin or other common fixatives) then you would be actually "fixing" the prion's ability to NOT be inactivated. Prions are nasty little beasties, and best left to dedicated labs that do nothing else. Please refer to the link below for the WHO pdf document which will give you procedures for handling prion infected patient's and tissues. http://www.who.int/csr/resources/publications/bse/whocdscsraph2003.pdf Section 8.2.2 directly addresses the Histopathology techniques to be used when handling these tissues. So much about these self replicating proteins is unknown, and more forms are added to the list all the time. Currently, the CDC recognizes 5 human forms, including, Creutzfeldt-Jakob Disease (CJD) Variant Creutzfeldt-Jakob Disease (vCJD) Gerstmann-Straussler-Scheinker Syndrome Fatal Familial Insomnia Kuru There are also 6 Animal Prion Diseases: Bovine Spongiform Encephalopathy (BSE) Chronic Wasting Disease (CWD) Scrapie Transmissible mink encephalopathy Feline spongiform encephalopathy Ungulate spongiform encephalopathy See the CDC link below for more information http://www.cdc.gov/ncidod/dvrd/prions/ and above all, Please, Please, Please be very careful. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --- On Thu, 1/15/09, Joe Hardin wrote: From: Joe Hardin Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From lblazek <@t> digestivespecialists.com Thu Jan 15 14:13:56 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 15 14:12:39 2009 Subject: [Histonet] RE: New Dako IHC Instrument and T.P.I.D. In-Reply-To: <938F8EC5A524D34EB5796E23E52781D3039D3A435E@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D3039D3A435E@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E39081F8B74B5@IBMB7Exchange.digestivespecialists.com> Beth, We looked into several immuno stainers for over a year period and found that the Intellipath from Bio Care beat them all out. It has the ability to run multiple stains at one time and has a very flexible platform. You can continuously add cases and stat cases. The support we have received from tech support has been absolutely remarkable. Service for the instrument has been equally as great. Best of all the cost of the Intellipath was right. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, January 15, 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Dako IHC Instrument and T.P.I.D. I'm curious if anyone out there has any feedback or comments on the new Dako IHC Instrument, Autostainer Link 48 and/or their TPID (True Positive ID). We are in desparate need of replacing our current DAKO instruments, and are probably going to stick with DAKO again after looking at several other instruments. I just thought I check to see if anyone using it has anything to say. Beth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Thu Jan 15 14:26:58 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Jan 15 14:29:45 2009 Subject: [Histonet] prion contaminated tissue processing In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70402ADB2@LTA3VS011.ees.hhs.gov> References: <739925.22467.qm@web65707.mail.ac4.yahoo.com> <1CE1847DFEA0A647B1CCDE4108EA60A70402ADB2@LTA3VS011.ees.hhs.gov> Message-ID: <496F9C12.5050801@umdnj.edu> > exposure to bleach will kill 'em - last I heard. think about it... if that were the case, things like BSE and CJD wouldn't be able to taint meat-processing equipment, which is generally about as awash in bleach as anything could be, at least once per shift. From Jackie.O'Connor <@t> abbott.com Thu Jan 15 15:02:52 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jan 15 15:03:14 2009 Subject: [Histonet] prion contaminated tissue processing In-Reply-To: <496F9C12.5050801@umdnj.edu> Message-ID: You're right - "the last I heard" was about 15 years ago. It's interesting to hear the that things we learned back then - will still kill us today. Everything I know I learned from www.giantmicrobes.com. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 01/15/2009 02:26 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] prion contaminated tissue processing > exposure to bleach will kill 'em - last I heard. think about it... if that were the case, things like BSE and CJD wouldn't be able to taint meat-processing equipment, which is generally about as awash in bleach as anything could be, at least once per shift. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pattennj <@t> mail.nih.gov Thu Jan 15 15:36:48 2009 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Thu Jan 15 15:36:53 2009 Subject: [Histonet] Autoflourescence In-Reply-To: References: Message-ID: Hi- I am having a horrible time with autofluorescence in my human brain FFPE tissue. I have been using Sudan Black which helps a little, but the background is still pretty bad. The tissue is usually cut at 10-15um. Does anyone have any other suggestions? Has anyone tried photobleaching the tissue prior to staining with any success? I attempted this once with no luck... Any help would be very much appreciated! Thanks in advance. Nicole From anh2006 <@t> med.cornell.edu Thu Jan 15 15:46:42 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Jan 15 15:46:57 2009 Subject: [Histonet] Autoflourescence Message-ID: <1e9b7baa61d5.496f6872@med.cornell.edu> 10-15 um seems extremely thick for paraffin sections. Can you get thinner sections? Say no thicker than 6um? Unfortunately autofluorescence is a fact of life with FFPE sections, hence why people normally do not choose to do immunofluorescence on FFPE sections. Are you tethered to using immunofluorescence? There are alternatives. ----- Original Message ----- From: "Patten, Nicole (NIH/NIAAA) [F]" Date: Thursday, January 15, 2009 4:36 pm Subject: [Histonet] Autoflourescence > Hi- > > I am having a horrible time with autofluorescence in my human > brain FFPE > tissue. I have been using Sudan Black which helps a little, but the > background is still pretty bad. The tissue is usually cut at 10-15um. > > Does anyone have any other suggestions? Has anyone tried > photobleachingthe tissue prior to staining with any success? I > attempted this once > with no luck... > > Any help would be very much appreciated! Thanks in advance. > > Nicole > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bob.nienhuis <@t> gmail.com Thu Jan 15 15:54:01 2009 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Thu Jan 15 15:54:09 2009 Subject: [Histonet] Autoflourescence In-Reply-To: References: Message-ID: <45109da50901151354g198135bl97b03b191266e093@mail.gmail.com> The Wright Cell Imaging Facility at University Health Network in Toronto has compiled information on reducing autofluorescence from the Histonet archives into a booklet. Very useful. http://www.uhnres.utoronto.ca/facilities/wcif/fdownload2.html Bob On Thu, Jan 15, 2009 at 1:36 PM, Patten, Nicole (NIH/NIAAA) [F] < pattennj@mail.nih.gov> wrote: > Hi- > > I am having a horrible time with autofluorescence in my human brain FFPE > tissue. I have been using Sudan Black which helps a little, but the > background is still pretty bad. The tissue is usually cut at 10-15um. > > Does anyone have any other suggestions? Has anyone tried photobleaching > the tissue prior to staining with any success? I attempted this once > with no luck... > > Any help would be very much appreciated! Thanks in advance. > > Nicole > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jason.PALMER <@t> svhm.org.au Thu Jan 15 21:45:27 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Thu Jan 15 21:45:38 2009 Subject: [Histonet] thick paraffin sections for immunostaining References: Message-ID: We would like to do immunostaining on thick FFPE sections for neuronal markers such as tyrosine hydroxylase so that we may trace neurons contained in non-brain tissue. Section thickness will probably be in the order of 40-50 microns, though perhaps thicker too. I have searched histonet archives but have only found references to vibratome sections of brain tissue, wholemount embryos and free-floating sections that I assume were cut on a cryostat. Antibody penetration has been achieved using DMSO, ethanol extraction, acetone, triton-x 100 etc, but I was wondering if anybody had managed to use any of these methods successfully on thick paraffin sections of non-brain tissue - our tissue is predominantly connective / adipose in nature. It is possible that we could do vibratome sections if we had to, but the tissues have already been collected, processed and paraffin blocked and would rather not have to do further experiments and tissue collection. I did also note in the archives that in regular paraffin sections, antibody penetration and thus labelling with standard methods is only thought to be of the order of 2 microns. Many thanks, Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From ejschmid <@t> ucalgary.ca Fri Jan 16 09:12:50 2009 From: ejschmid <@t> ucalgary.ca (ejschmid@ucalgary.ca) Date: Fri Jan 16 09:13:29 2009 Subject: [Histonet] texas red Message-ID: <54910.68.147.51.95.1232118770.squirrel@68.147.51.95> I need my GMA plastic sections to fluoresce, that is the plastic itself. For the embedded tissue itself, I am using DAPI and Alexa Fluor 488. So the stain I choose for the plastic, which I need to see using fluorescence, cannot interfere with the DAPI or Alexa Fluor 488. This is basically a triple labeling problem. The third stain I am considering is Texas Red. The reason is that I can get it without it conjugated to something (like a secondary) that makes it even more expensive. I wouldn't use it to stain cells or tissues, but rather the plastic of the thin sections (I know that sounds weird but my needs require a little thinking outside the box). So my basic question about whether Texas Red is a good choice breaks down into two more specific questions: Will it work with the ALexa Fluor 488? Is there another choice for a third stain that anyone can think of that might be more suitable? Thanks! Eric University of Calgary From kmerriam2003 <@t> yahoo.com Fri Jan 16 09:14:24 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jan 16 09:14:29 2009 Subject: [Histonet] PhosStop and Complete and cryosections Message-ID: <779312.75086.qm@web50304.mail.re2.yahoo.com> Hi Everyone, ? Happy Friday! ? I am looking to use PhosStop and Complete (both from Roche) in my fixative for cryosections (and cytospins).? I have used in in the past in formalin for FFPE xenografts, and now I want to use it as a cryo-section fixative.? What I want to know is: has anyone tried this? is this solution re-usable (for more than one rack of slides); will I need a fresh batch for each rack or can I re-use it for several racks during the same day.? They are both supposed to be stable, once dissolved, at?4C for 1-month.? I don't know how much "depletion" or inactivation?will occur if I use this reagent repeated during the?day (it would probably only be about 8 racks of slides in the fixative) should I keep it cold (ie - fix the slides @ 4C) or can I use it at Room Temp (fix the slides at RT) Any insight would be great! ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From burch007 <@t> mc.duke.edu Fri Jan 16 10:25:14 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Jan 16 10:26:10 2009 Subject: [Histonet] NC Society for Histopathology Technologists 2009 Meeting Message-ID: The North Carolina Society for Histopathology Technologists invites you to our 2009 meeting to be held May first and second at the DoubleTree Biltmore Hotel in beautiful Asheville North Carolina. Call the DoubleTree Biltmore Hotel at 1-800-222-8733 for special room rates and reservations or visit them on line at www.biltmoreasheville.doubletree.com. Make sure you mention NCSHT. Our program is nearly finished and will be posted on the NSH web site next month. We look forward to seeing you in Asheville The officers and board members of NCSHT From anitaibsc <@t> aol.com Fri Jan 16 11:54:34 2009 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Fri Jan 16 11:55:01 2009 Subject: [Histonet] Methylene Blue Message-ID: <8CB461AA060373A-B10-CFB@WEBMAIL-DC11.sysops.aol.com> Hello Netters, We are looking for a recipe and instructions for Methylene Blue counterstain and histology reagent, can anyone help. Thank you. Anita Email: anitaIBSC@aol.com www.ImmunoBioScience.com From rjbuesa <@t> yahoo.com Fri Jan 16 15:20:52 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 16 15:20:56 2009 Subject: [Histonet] Methylene Blue In-Reply-To: <8CB461AA060373A-B10-CFB@WEBMAIL-DC11.sysops.aol.com> Message-ID: <855347.83928.qm@web65709.mail.ac4.yahoo.com> Anita: There are scores of formulas?using methylene blue, but the majority use what is called a "saturated" solution which is?about 4.5%? aq. sol. Regarding the action time it is better for you to test some times and select the one is more suitable for your purposes although it is usually used from 4-5 minutes. Ren? J. --- On Fri, 1/16/09, anitaibsc@aol.com wrote: From: anitaibsc@aol.com Subject: [Histonet] Methylene Blue To: Histonet@lists.utsouthwestern.edu Date: Friday, January 16, 2009, 12:54 PM Hello Netters, We are looking for a recipe and instructions for Methylene Blue counterstain and histology reagent, can anyone help. Thank you. Anita Email: anitaIBSC@aol.com www.ImmunoBioScience.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 <@t> gmail.com Fri Jan 16 16:52:23 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Fri Jan 16 16:52:30 2009 Subject: [Histonet] New Dako IHC Instrument and T.P.I.D. Message-ID: Beth: I evaluated the AS-Link/TPID and wasn't too impressed. Its the same basic machine with a new outside/skin and the only difference is that in can read barcode labels on the reagents. To me, the postive-ID part should be handles by the LIS, not the staining system. One part of the deal that really concerned me is that Dako is pushing a new detectionsystem called Flex, which costs a lot more than Envision plus. And they want to bill our lab on a per-slide basis, which seems fishy to me. Ican send you my cost analysis if you want. Next stop: Biocare's intellipath. Sally -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto: histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, January 15, 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Dako IHC Instrument and T.P.I.D. I'm curious if anyone out there has any feedback or comments on the new Dako IHC Instrument, Autostainer Link 48 and/or their TPID (True Positive ID). We are in desparate need of replacing our current DAKO instruments, and are probably going to stick with DAKO again after looking at several other instruments. I just thought I check to see if anyone using it has anything to say. Beth From DKnutson <@t> primecare.org Sat Jan 17 14:47:24 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Sat Jan 17 14:47:08 2009 Subject: [Histonet] Coverslippers Message-ID: <4F0B7161A6CD524FAD8017D52E155340090679FE@exchangent> Our lab is interested in purchasing a coverslipping instrument and would like to hear from the rest of you on what type is a good one to purchase? Also, what are the pros and cons on glass coverslips vs plastic coverslips vs film for coverslips. Thank you in advance for sharing your knowledge on this topic. Deanne Knutson HTL,MT (ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Office: 530-6730 dknutson@primecare.org From jqb7 <@t> cdc.gov Sat Jan 17 15:02:17 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Sat Jan 17 15:06:03 2009 Subject: [Histonet] Coverslippers References: <4F0B7161A6CD524FAD8017D52E155340090679FE@exchangent> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF780@LTA3VS011.ees.hhs.gov> We prefer the glass coverslippers and currently have a Leica CV5030 and have had no problems. We have just purchased the Sakura glass coverslipper as well. It has not yet been installed but I have heard very good things about it. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Knutson, Deanne Sent: Sat 1/17/2009 3:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Coverslippers Our lab is interested in purchasing a coverslipping instrument and would like to hear from the rest of you on what type is a good one to purchase? Also, what are the pros and cons on glass coverslips vs plastic coverslips vs film for coverslips. Thank you in advance for sharing your knowledge on this topic. Deanne Knutson HTL,MT (ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Office: 530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CrochiereSteve <@t> aol.com Sat Jan 17 17:13:32 2009 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Sat Jan 17 17:13:43 2009 Subject: [Histonet] ihc stainer Message-ID: Hi, I looked into many and found the Intellipath from Biocare medical the way to go. Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 From invite+kgaxsgex <@t> facebookmail.com Sat Jan 17 19:30:21 2009 From: invite+kgaxsgex <@t> facebookmail.com (Mary T. Lloyd Hodges) Date: Sat Jan 17 19:30:25 2009 Subject: [Histonet] Check out my Facebook profile Message-ID: Hi HistoNet, I set up a Facebook profile where I can post my pictures, videos and events and I want to add you as a friend so you can see it. First, you need to join Facebook! Once you join, you can also create your own profile. Thanks, Mary To sign up for Facebook, follow the link below: http://www.facebook.com/p.php?i=1578078899&k=45D6YYW4WW3M51DGSJXTWV&r From nefff <@t> staff.uni-marburg.de Sun Jan 18 09:14:00 2009 From: nefff <@t> staff.uni-marburg.de (nefff@staff.uni-marburg.de) Date: Sun Jan 18 09:14:17 2009 Subject: [Histonet] autoradiography Message-ID: <20090118161400.qhfgxsjnswcocc0g@home.staff.uni-marburg.de> Dear Histonetters, I'm supposed to do autoradiography of mice brains treated with tritium labelled antibodies, but I've never done it before. Could anyone provide me with a protocol or a link or a reference? Thanks in advance, Frauke From rjbuesa <@t> yahoo.com Sun Jan 18 09:44:29 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 18 09:44:33 2009 Subject: [Histonet] Coverslippers In-Reply-To: <4F0B7161A6CD524FAD8017D52E155340090679FE@exchangent> Message-ID: <953148.87701.qm@web65710.mail.ac4.yahoo.com> Deanne: Try to get a demo from Sakura. Regarding glass vs. film: ? FILM: faster and slides can be filed immediately; after YEARS the film may become loose, but only if it was placed with less than needed xylene. ? GLASS: better for photomicrography IF you use apochromat high power dry objective and glass remains better in the long time. They have a higher failure rate (more interruptions in the work flow). Sometimes slides need to wait flat for days before being filed. ? I personally prefer film, the net result outweigts its drawbacks. Ren? J.? --- On Sat, 1/17/09, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] Coverslippers To: "'histonet@lists.utsouthwestern.edu'" Date: Saturday, January 17, 2009, 3:47 PM Our lab is interested in purchasing a coverslipping instrument and would like to hear from the rest of you on what type is a good one to purchase? Also, what are the pros and cons on glass coverslips vs plastic coverslips vs film for coverslips. Thank you in advance for sharing your knowledge on this topic. Deanne Knutson HTL,MT (ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Office: 530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TraceyG <@t> adhb.govt.nz Sun Jan 18 13:44:56 2009 From: TraceyG <@t> adhb.govt.nz (Tracey Gunn) Date: Sun Jan 18 13:45:03 2009 Subject: [Histonet] New IHC/ISH machine Message-ID: Hi all, I have watched with interest the discussions recently on new IHC/ISH autostainers as our current contract is up for renewal. Does anyone have the XMatrx from Biogenex? I have read a small amount about the Intellipath but does this machine have onboard HIER and dewaxing? We are also looking at the Benchmark Ultra and Dako Autostainer, and we are currently running the Bond Max machines which are still in the picture. I am in New Zealand so servicing and reagent availability are also very important. Any thoughts would be greatly appreciated. Thanks, Tracey. Tracey Gunn Section Leader - Histology LabPlus Auckland City Hospital (09) 307 4949 ext 6121 From fatima.ferreirinha <@t> gmail.com Mon Jan 19 05:53:16 2009 From: fatima.ferreirinha <@t> gmail.com (=?ISO-8859-1?Q?F=E1tima_Ferreirinha?=) Date: Mon Jan 19 05:53:25 2009 Subject: [Histonet] rat NMJ immunostaining Message-ID: <954393540901190353i381bab34r1c6863e7afcc487@mail.gmail.com> Hello everybody, I'm having trouble obtaining immunofluorescence staining on rat diaphragm motor endplates (fixed with 4% PFA). I have no staining at all! Motor enplates are there as I can see them because I use an additional staining with a peptide (bungarotoxin) labelled with TRITC. I have already immunostained mouse motor endplates (of the foot pads) with the same technique and I had no trouble!... Those antibodies work on rat tissues because I've already tried them on other rat tissues with no problem. I've already tried several technical modifications with no luck. I don't understand what could be the problem! Is there any difference between rat and mouse motor endplates? Could anyone give some hint, please? Thank you, F?tima From BMolinari <@t> heart.thi.tmc.edu Mon Jan 19 06:10:17 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Jan 19 06:10:21 2009 Subject: [Histonet] Coverslippers In-Reply-To: <953148.87701.qm@web65710.mail.ac4.yahoo.com> References: <4F0B7161A6CD524FAD8017D52E155340090679FE@exchangent> <953148.87701.qm@web65710.mail.ac4.yahoo.com> Message-ID: I use the Sakura Tissue Tek Glas and love it. Low maintenance, easy to clean and simple to program. >From what I understand if you have a high production of slides glass may be too slow . Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue MC1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, January 18, 2009 9:44 AM To: histonet@lists.utsouthwestern.edu; Knutson, Deanne Subject: Re: [Histonet] Coverslippers Deanne: Try to get a demo from Sakura. Regarding glass vs. film: ? FILM: faster and slides can be filed immediately; after YEARS the film may become loose, but only if it was placed with less than needed xylene. ? GLASS: better for photomicrography IF you use apochromat high power dry objective and glass remains better in the long time. They have a higher failure rate (more interruptions in the work flow). Sometimes slides need to wait flat for days before being filed. ? I personally prefer film, the net result outweigts its drawbacks. Ren? J.? --- On Sat, 1/17/09, Knutson, Deanne wrote: From: Knutson, Deanne Subject: [Histonet] Coverslippers To: "'histonet@lists.utsouthwestern.edu'" Date: Saturday, January 17, 2009, 3:47 PM Our lab is interested in purchasing a coverslipping instrument and would like to hear from the rest of you on what type is a good one to purchase? Also, what are the pros and cons on glass coverslips vs plastic coverslips vs film for coverslips. Thank you in advance for sharing your knowledge on this topic. Deanne Knutson HTL,MT (ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Office: 530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jan 19 07:57:10 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jan 19 07:57:13 2009 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors 1-19-09 Message-ID: Hello Histonetters, I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. The compensation packages are excellent, the work is challenging and the sky is the limit. The positions are of course full time and permanent. Here are my leadership positions: Histology Manager ? Maryland, West of DC Research Histology Manager ? Boston, MA Night Shift Histology Supervisor ? Ft. Lauderdale, FL Histology Supervisor - Los Angeles, CA Gross Room Supervisor ? Austin, TX If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam New for 2009 RELIA is offering a $500 referral bonus for anyone you refer and I place and a $500 hiring bonus if I place you! There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> From kynemitz <@t> travmax.com Mon Jan 19 08:18:01 2009 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Mon Jan 19 08:18:07 2009 Subject: [Histonet] Perm Histo position in San Antonio, TX Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71F457BA1@exbk2.maxhealth.com> Happy Monday histonet! One of my clients in San Antonio has notified me that they are in search of a full time, permanent Histo tech for their night shift. -ASCP preferred -Sign on bonus of $3500 If you or anyone you know would be interested, please contact me. Thanks! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare * 888.800.1855 or 813.371.5175 7 800.294.1248 Search our jobs or apply online at: www.TravelMaxAllied.com , www.tmaxnursing.com From NMargaryan <@t> childrensmemorial.org Mon Jan 19 09:51:30 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Jan 19 09:55:01 2009 Subject: [Histonet] chicks' embryos and LCM Message-ID: Dear histoneters, I am going to start the LCM work on chicks' embryos and now more questions are coming up :-) 1. What kind of brand of blades will you suggest to use for crysectioning (Catalog ## will be very good)? 2. After placing glass slides in LCM what kind of caps will you suggest using to capture peaces: CupSureHS or Macro? 3. What is minimum amount of peaces I have to capture to get good quality of RNA? Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From bliven.laura <@t> marshfieldclinic.org Mon Jan 19 12:08:19 2009 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Mon Jan 19 12:08:26 2009 Subject: [Histonet] MHC Class 1 Antibody Message-ID: <200901191808.n0JI8M9n024108@mailhost2.mfldclin.edu> I'm looking for an MHC Class 1 antibody for staining on frozen muscle sections. Would prefer an unconjugated antibody. Anyone performing this test willing to suggest an antibody and company? Thanks much, Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From freckles9660 <@t> yahoo.com Mon Jan 19 12:20:25 2009 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Mon Jan 19 12:20:31 2009 Subject: [Histonet] Validation Message-ID: <695891.54867.qm@web32506.mail.mud.yahoo.com> Fellow HT's: I am putting together?a DAKO IHC instrument for performing IHC's.? I would like any information or examples of antibody validation forms that others are using to validate their antibodies.?Examples would be greatly appreciated or a link to where I can get a sample to look at and tweek. Thanks!! Karla Arrington, HT(ASCP) From smehta <@t> magenbiosciences.com Mon Jan 19 12:52:41 2009 From: smehta <@t> magenbiosciences.com (Shanu Mehta) Date: Mon Jan 19 12:52:51 2009 Subject: [Histonet] Problems with sectioning hairy skin Message-ID: <4C78CF3C61E6A640888226885709EFE99E34CB@server01.magen.local> Hello All, I have been sectioning some fresh frozen hairy human skin on the cryostat and I am having too much trouble getting decent sections. The sections seem to tear up and the epidermis & the dermis appear to separate. My technique: I embed dermis side down in OCT compound and then place the mold onto some dry ice until the mold solidifies. I then transfer the molds to a -80?C freezer until they are ready to cut. Next I let the blocks come to cryostat temperature in the chamber. For cutting, I turn the block at a 90? angle meaning that both the dermis & the epidermis get cut in the same plane. I usually cut at a Chamber Temp of -19 & an Object Temp of -21. My cutting angle is between 3 & 5. I use low profile blades. I trim most of the fat that may be present in the skin, but there may be residual fat pockets that I may have missed! This morning I talked to the technical staff at Leica and I was suggested to change the Chamber Temp to -21 to match that of the Object Temp. Also, I was asked to try with an angle of 5 or greater than 5 (as high as 8 or 9). I tried a range of temperatures and cutting angles, all in vain. I am still not happy with the quality of sections I am getting! Any suggestions on what I could do? I greatly appreciate any comments. Thanks to all, Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com From MElliott <@t> mrl.ubc.ca Mon Jan 19 12:54:59 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Mon Jan 19 12:55:40 2009 Subject: [Histonet] PRRX1 antibody from Abcam Message-ID: <49745C03.11C6.00D6.0@mrl.ubc.ca> Hi Everyone I was wondering if anyone has tried using this antibody for IHC, either paraffin or frozen. Datasheet says it hasn't yet been tested for IHC, only Western Blots. Does anyone have an alternate to the above that works? Thanks Mark Elliott in sunny, cold Vancouver, BC-no fog today!-first time in almost a week when we can see the sky! ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From gayle.callis <@t> bresnan.net Mon Jan 19 16:31:18 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Jan 19 16:31:27 2009 Subject: [Histonet] Re: human skin frozen sections Attn Shanu Message-ID: <000101c97a85$a6208d40$f261a7c0$@callis@bresnan.net> Shanu, You can also fold the skin slightly into a V shape so the hair is on the INSIDE of the fold. Hold this V folded tissue in forceps, embed in OCT, then allow to start freezing, then release the hair, add more OCT to the unfrozen OCT. We have frozen bovine (very hairy!) and mouse skin into this configuration, and after freezing, be sure to equilibrate your block for 20 minutes or so, to the cutting temperature inside the cryostat. Be sure you are not taking a freshly mounted block from a very cold area of cryostat and try to cut it at -21C unless you have the tissue equilibrated to that cutting temperature. We have a (beloved!)Cryocut 1850, and the freezing plates where the Peltier device is located are always 10 degrees colder than the object/blade temperature. We set our mounted blocks next to the microtome for equilibration to the cutting temperature (takes approx 20 minutes). We have the blade, object and chamber temperature all the same, -20C although you can try colder or warmer. Equilibration from freezer to sectioning temperature goes faster if you mount the tissue on the metal disk, then let it equilibrate next to microtome. Waiting for things to warm up, freeze onto disk, then warm up to cutting temperature tries ones patience. We temperature tested to know where all the warm and cold spots in the chamber are located, and use the warm areas which are usually the object/cutting temperature, for block equilibration. We orient the V shaped tissue so the blade meets the bottom of the v shape first, and sectioning proceeds through the tissue with the dermis/epidermis sectioned last. We prefer high profile blades over low profile for more stability, but just as sharp. We do not increase the blade angle. We have used Tissue Tek AccuEdge high profile blades, set at 6, one tick past 5, in our cryostats for over 20 years - they are excellent for frozen sections, and with skin, change the edge frequently for the sharpest blade. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 You wrote: I have been sectioning some fresh frozen hairy human skin on the cryostat and I am having too much trouble getting decent sections. The sections seem to tear up and the epidermis & the dermis appear to separate. My technique: I embed dermis side down in OCT compound and then place the mold onto some dry ice until the mold solidifies. I then transfer the molds to a -80?C freezer until they are ready to cut. Next I let the blocks come to cryostat temperature in the chamber. For cutting, I turn the block at a 90? angle meaning that both the dermis & the epidermis get cut in the same plane. I usually cut at a Chamber Temp of -19 & an Object Temp of -21. My cutting angle is between 3 & 5. I use low profile blades. I trim most of the fat that may be present in the skin, but there may be residual fat pockets that I may have missed! This morning I talked to the technical staff at Leica and I was suggested to change the Chamber Temp to -21 to match that of the Object Temp. Also, I was asked to try with an angle of 5 or greater than 5 (as high as 8 or 9). I tried a range of temperatures and cutting angles, all in vain. I am still not happy with the quality of sections I am getting! Any suggestions on what I could do? I greatly appreciate any comments. Thanks to all, Shanu From godsgalnow <@t> aol.com Tue Jan 20 09:34:32 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Jan 20 09:34:44 2009 Subject: [Histonet] p16 Message-ID: <8CB492BB9B39299-8FC-321@WEBMAIL-MY32.sysops.aol.com> Anyone know where I can get an IVD p16? Roxanne From jrobertson <@t> pathologysciences.com Tue Jan 20 11:32:32 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Tue Jan 20 11:32:38 2009 Subject: [Histonet] p16 In-Reply-To: <8CB492BB9B39299-8FC-321@WEBMAIL-MY32.sysops.aol.com> References: <8CB492BB9B39299-8FC-321@WEBMAIL-MY32.sysops.aol.com> Message-ID: <518CD6920AA7154193CBE5977CD88073177E41@psmgsrv2.PSMG.local> MtM Laboratories holds the exclusive rights to p16 now. mtmlabs.com will get you further information. Jodie Robertson, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Tuesday, January 20, 2009 7:35 AM To: histonet@pathology.swmed.edu Subject: [Histonet] p16 Anyone know where I can get an IVD p16? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AJohnson <@t> aipathology.com Tue Jan 20 11:55:13 2009 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Tue Jan 20 11:55:21 2009 Subject: [Histonet] Frozen section staining line Message-ID: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFC@SERV001> How are labs out there labeling their frozen section staining line? Do you label each coplin jar individually or can there be some way to combine the labeling? Thanks Amy Johnson Associates in Pathology From tbraud <@t> holyredeemer.com Tue Jan 20 12:28:20 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 20 12:28:28 2009 Subject: [Histonet] RE: Frozen Section Line Labeling In-Reply-To: <1baf2cb6000210e2@HolyRedeemer.com> Message-ID: We label each container (and lid, cause it looks nicer and keeps a nasty eosin lid from going other places) separately, using a Brother p-touch labeler. These labels not only look great, crisp clear and easy to read, but are laminated and will hold up to some solvent exposure, and lots of washing. Ever since purchasing one of these little gems, our labeling in the lab is wonderful! Here is the link to the site to tell you about them. http://www.brother-usa.com/Ptouch/whatisit/ Happy Labeling, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 8. Frozen section staining line (Amy Johnson) From: "Amy Johnson" Subject: [Histonet] Frozen section staining line How are labs out there labeling their frozen section staining line? Do you label each coplin jar individually or can there be some way to combine the labeling? Thanks Amy Johnson Associates in Pathology --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From anh2006 <@t> med.cornell.edu Tue Jan 20 12:29:22 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Jan 20 12:29:32 2009 Subject: [Histonet] EphB4 & ephrinB2 Message-ID: Is anyone staining these proteins successfully in mouse tissue? If so, where are your antibodies from? Thanks. -- From gu.lang <@t> gmx.at Tue Jan 20 12:30:42 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 20 12:30:57 2009 Subject: AW: [Histonet] Frozen section staining line In-Reply-To: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFC@SERV001> References: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFC@SERV001> Message-ID: We have a system, where you can put the green plastic jars in metaltrays. This trays may have three or more places for the jars. The trays are labelled. They look like these on this website: http://www.medite-group.at/catalog/product_info.php?cPath=36_115&products_id =230 Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amy Johnson Gesendet: Dienstag, 20. J?nner 2009 18:55 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Frozen section staining line How are labs out there labeling their frozen section staining line? Do you label each coplin jar individually or can there be some way to combine the labeling? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Jan 20 12:33:03 2009 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Jan 20 12:34:23 2009 Subject: [Histonet] RE: Frozen section staining line In-Reply-To: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFC@SERV001> References: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFC@SERV001> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D304E46B1@LRGHEXVS1.practice.lrgh.org> We have both the line and jars labeled. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson [AJohnson@aipathology.com] Sent: Tuesday, January 20, 2009 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section staining line How are labs out there labeling their frozen section staining line? Do you label each coplin jar individually or can there be some way to combine the labeling? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From dwbrawn <@t> ihis.org Tue Jan 20 12:39:52 2009 From: dwbrawn <@t> ihis.org (Danny Brawn) Date: Tue Jan 20 12:40:27 2009 Subject: [Histonet] cryostat sectioning hairy skin Message-ID: Hi, I have used a Leica cryostat for several years and found that for most types of tissue, it cuts best at an angle set at 0. I am interested in knowing how long it takes for the tissue to warm up from -80 to -20. Danny Brawn Prince Edward Island Canada ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From AJohnson <@t> aipathology.com Tue Jan 20 13:01:53 2009 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Tue Jan 20 13:02:01 2009 Subject: [Histonet] Disinfecting the Cryostat Message-ID: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFD@SERV001> Hello again.....going thru the new CAP checklist, we have found that if the cryostat is used on a daily basis it should be disinfected at least once a week. My question is how are others doing this disinfection without compromising the use during the day, Thanks again Amy Johnson From settembr <@t> umdnj.edu Tue Jan 20 13:08:47 2009 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jan 20 13:12:40 2009 Subject: [Histonet] MHC Class 1 Antibody Message-ID: Hello Laura, We use Dako's Mouse Monoclonal Anti-Human HLA-ABC antigen Cat.# M0736 for MHC Class 1 I just looked online and the spec sheet is not available, but here is what the spec sheet says that I have in front of me: Specificity: "Anti-HLA-ABC Class I Antigen," (clone) "W6/32 is directed against a monomorphic epitope on the 45 kDa polypeptide products of the HLA-A, -B and -C loci. These glycosylated polypeptides are non-covalently associated with B-2 microglobulin, constituting the HLA-A, -B, -C or class I antigen of the human major histocompatibility complex (MHC)" I use a acetone fixation of 4 minutes, then buffer then staining. Hope this helps. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Bliven, Laura" 01/19/09 1:08 PM >>> I'm looking for an MHC Class 1 antibody for staining on frozen muscle sections. Would prefer an unconjugated antibody. Anyone performing this test willing to suggest an antibody and company? Thanks much, Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From b-frederick <@t> northwestern.edu Tue Jan 20 13:22:38 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jan 20 13:22:57 2009 Subject: [Histonet] Frozen section staining line In-Reply-To: <4D6F15688B0DD34AA60F1C9A076BE57C26FEFC@SERV001> Message-ID: <000001c97b34$79178470$d00f7ca5@lurie.northwestern.edu> Lucky for us that cut research frozens and don't have to rush- we stick them on the autostainer starting in water prior to Hemo (we fix in 95% as we cut) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Tuesday, January 20, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section staining line How are labs out there labeling their frozen section staining line? Do you label each coplin jar individually or can there be some way to combine the labeling? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Jan 20 13:30:51 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jan 20 13:31:01 2009 Subject: [Histonet] xylene recyclers Message-ID: Folks, I am again in the market to get a xylene recycler, would love to get a kindly used one or if a vendor has a great deal for a small poor lab feel free to contact me. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From cjbulmer <@t> sbcglobal.net Tue Jan 20 14:15:18 2009 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Tue Jan 20 14:15:27 2009 Subject: [Histonet] Job posting Message-ID: <653709.92739.qm@web82301.mail.mud.yahoo.com> Central Texas Pathology Laboratory in Waco, TX has a full time position available for an IHC technician.? Must be HT(ASCP) registered.? CTPL offers excellent benefits along with competitive salary.? Contact me for more information. ? Cindy ? ? Cynthia Bulmer, HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX 254-752-9621, ext 447 From smehta <@t> magenbiosciences.com Tue Jan 20 14:18:20 2009 From: smehta <@t> magenbiosciences.com (Shanu Mehta) Date: Tue Jan 20 14:18:25 2009 Subject: [Histonet] RE: Skin Frozen Sections In-Reply-To: References: Message-ID: <4C78CF3C61E6A640888226885709EFE99E3542@server01.magen.local> Thank you Gayle Callis, Alexandra Meinl & Lee Dickey for the suggestions. I will try to incorporate as many suggestions as possible. However, 1) Regarding folding the skin into a V shape: I cut a 5mm punch biopsy into half (one half for frozen & the other for paraffin embedding). So this leaves me with a very small piece of semi circle to work with. I will try though to fold it the next time I do some embedding! 2) I do not shave the skin - I rather cut off as much hair as possible. So in the end, I have tiny hair with the biopsy. Most of the other points made are generally followed but I will be extra careful to follow them all! Thanks a lot. I really appreciate all the comments. Shanu ------------ ________________________________ From: Lee E. Dickey [mailto:ledickey@Coretech-holdings.com] Sent: Tuesday, January 20, 2009 2:45 PM To: Shanu Mehta Subject: Skin Frozen Sections Shanu I agree with all of the suggestions from Gayle Callis. I used to cut a lot of frozen sections from research animals to renal biopsies, skin punch biopsies and Mohs. A couple things I will add are: 1) Make sure that all of the tissue being cut is surrounded by frozen section embedding media; and 2) make sure the section is no longer attached to the cutting blade edge before you pick up the section. The epidermis will stick to the blade edge and pull out of the section 99% of the time. The frozen section embedding media will provide a border and also help from separating the epidermal layer from the dermis. Take the extra few seconds to run an applicator stick along the blade edge very lightly and separate the section from the blade and then pick up on a slide. If you push the slide to close to the flat part of the blade the epidermis again will freeze to the blade and pull out of the section. Date: Mon, 19 Jan 2009 13:52:41 -0500 From: "Shanu Mehta" Subject: [Histonet] Problems with sectioning hairy skin To: Message-ID: <4C78CF3C61E6A640888226885709EFE99E34CB@server01.magen.local> Content-Type: text/plain; charset="iso-8859-1" Hello All, I have been sectioning some fresh frozen hairy human skin on the cryostat and I am having too much trouble getting decent sections. The sections seem to tear up and the epidermis & the dermis appear to separate. My technique: I embed dermis side down in OCT compound and then place the mold onto some dry ice until the mold solidifies. I then transfer the molds to a -80?C freezer until they are ready to cut. Next I let the blocks come to cryostat temperature in the chamber. For cutting, I turn the block at a 90? angle meaning that both the dermis & the epidermis get cut in the same plane. I usually cut at a Chamber Temp of -19 & an Object Temp of -21. My cutting angle is between 3 & 5. I use low profile blades. I trim most of the fat that may be present in the skin, but there may be residual fat pockets that I may have missed! This morning I talked to the technical staff at Leica and I was suggested to change the Chamber Temp to -21 to match that of the Object Temp. Also, I was asked to try with an angle of 5 or greater than 5 (as high as 8 or 9). I tried a range of temperatures and cutting angles, all in vain. I am still not happy with the quality of sections I am getting! Any suggestions on what I could do? I greatly appreciate any comments. Thanks to all, Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com Lee Lee E. Dickey Manager, Distribution and OEM Sales Biosystems Division Leica Biosystems St. Louis LLC 5918 Evergreen Blvd. St. Louis, MO 63134 United States of America telelphone: +1-866-737-2540 Ext. 323 home office: +1-866-737-2540 Ext. 343 mobile: +1-270-839-2791 facsimile: +1-314-522-6360 Email: lee.dickey@leica-microsystems.com Website: www.mccormickscientific.com From Herrick.James <@t> mayo.edu Tue Jan 20 16:15:02 2009 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Tue Jan 20 16:15:11 2009 Subject: [Histonet] TRAP Staining Protocol Message-ID: <4F820D0A1054E6478FD124A9BE03397271F49B@MSGEBE35.mfad.mfroot.org> Hello everyone, I have a question regarding a good TRAP stain protocol. We have pretty good luck using our protocol on human iliac crest biopsies, but the stain tends to fade to almost nothing overnight, when we use it on rat and mouse femurs, vertebrae, etc.. We do see some fading in our iliac crest biopsies, but it is not as severe as the animal specimens. Our specimens are embedded in GMA or MMA and the constituents of our protocol consist of pararosanilin (Basic Red), Acetate Buffer (solutions A & B), Naphthol AS-TR Phosphate and Manganese Chloride. We incubate at 37?C for 60 to 120 minutes, rinse with dH2O, dehydrate through graded alcohols and coverslip with Eukitt mounting medium. Does anyone know why our stains are fading or would you possibly have a different protocol that we can try? Any suggestions you may have would be greatly appreciated. Thanks for your help. Jim From ratliffjack <@t> hotmail.com Tue Jan 20 16:28:26 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Jan 20 16:28:31 2009 Subject: [Histonet] TRAP Staining Protocol In-Reply-To: <4F820D0A1054E6478FD124A9BE03397271F49B@MSGEBE35.mfad.mfroot.org> References: <4F820D0A1054E6478FD124A9BE03397271F49B@MSGEBE35.mfad.mfroot.org> Message-ID: James, Don't dehydrate to xylenes and/or coverslip with Eukitt. After you finish with your counterstain and rinse in water, blot the slide dry and mount with an aqueous mounting medium (Aqua Polymount - Polysciences). Jack Ratliff > Date: Tue, 20 Jan 2009 16:15:02 -0600> From: Herrick.James@mayo.edu> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] TRAP Staining Protocol> > Hello everyone,> > I have a question regarding a good TRAP stain protocol. We have pretty good luck using our protocol on human iliac crest biopsies, but the stain tends to fade to almost nothing overnight, when we use it on rat and mouse femurs, vertebrae, etc.. We do see some fading in our iliac crest biopsies, but it is not as severe as the animal specimens.> > Our specimens are embedded in GMA or MMA and the constituents of our protocol consist of pararosanilin (Basic Red), Acetate Buffer (solutions A & B), Naphthol AS-TR Phosphate and Manganese Chloride. We incubate at 37?C for 60 to 120 minutes, rinse with dH2O, dehydrate through graded alcohols and coverslip with Eukitt mounting medium. Does anyone know why our stains are fading or would you possibly have a different protocol that we can try? Any suggestions you may have would be greatly appreciated. Thanks for your help.> > Jim> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill.mcmanus <@t> usu.edu Tue Jan 20 16:35:20 2009 From: bill.mcmanus <@t> usu.edu (Bill McManus) Date: Tue Jan 20 16:35:29 2009 Subject: [Histonet] texas red In-Reply-To: <54910.68.147.51.95.1232118770.squirrel@68.147.51.95> References: <54910.68.147.51.95.1232118770.squirrel@68.147.51.95> Message-ID: <1A04FCAA-394E-475F-88F5-F449936BA5FF@usu.edu> Eric: Texas Red will interfere with the Alexi Flour488. Methylene blue would work better. Bill On Jan 16, 2009, at 8:12 AM, ejschmid@ucalgary.ca wrote: > I need my GMA plastic sections to fluoresce, that is the plastic > itself. > For the embedded tissue itself, I am using DAPI and Alexa Fluor 488. > So > the stain I choose for the plastic, which I need to see using > fluorescence, cannot interfere with the DAPI or Alexa Fluor 488. > > This is basically a triple labeling problem. The third stain I am > considering is Texas Red. The reason is that I can get it without it > conjugated to something (like a secondary) that makes it even more > expensive. I wouldn't use it to stain cells or tissues, but rather the > plastic of the thin sections (I know that sounds weird but my needs > require a little thinking outside the box). > > So my basic question about whether Texas Red is a good choice breaks > down > into two more specific questions: Will it work with the ALexa Fluor > 488? > Is there another choice for a third stain that anyone can think of > that > might be more suitable? > > Thanks! > Eric > University of Calgary > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Bill McManus Western Dairy Center Utah State University bill.mcmanus@usu.edu From tgenade <@t> gmail.com Wed Jan 21 06:25:23 2009 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Wed Jan 21 06:27:44 2009 Subject: [Histonet] BrdU, CldU and IdU nucleotide labeling Message-ID: Hi all, I would like to label slow and fast cycling progenitor cells using double nucleoside analog labeling. Can BrdU be distinguished from CldU and/or IdU? Maslov et al (J. Neurosci, 24:1726--1733, 2004) used anti-BrdU antibodies to show both. I assume, one stains one slide of BrdU only treated tissue with anti-BrdU to identify one set of progenitors, and then stains the slides of tissue treated with both (for example) BrdU and CldU and then substracts the first result from the last to get the difference? Or are there specific antibodies to BrdU, CldU and IdU that are available? Can anyone supply a copy of Aten et al (Meth. Cell Biol. 41:317--326, 1994)? Its the reference for the original protocol---unfortunately not available to me. :-( A protocol would be just as welcome. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 From yvan_lindekens <@t> yahoo.com Wed Jan 21 07:16:25 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Wed Jan 21 07:16:30 2009 Subject: [Histonet] Roth Rotiplast paraffin Message-ID: <781935.45470.qm@web110202.mail.gq1.yahoo.com> Anyone using this paraffin? What are your experiencies with it? How does it compare to f.e. Paraplast? It seems to be the cheapest paraffin availlable here. Thanks in advance! From SharonC <@t> celligent.net Wed Jan 21 10:25:32 2009 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Wed Jan 21 10:25:38 2009 Subject: [Histonet] Obtaining control tissue Message-ID: Hello histonetters! I am looking at obtaining control tissue for special stain controls and IHC controls. I think there is a tissue bank but I do not know who to contact. Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 sharonc@celligent.net From ree3 <@t> leicester.ac.uk Wed Jan 21 10:48:01 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Jan 21 10:48:08 2009 Subject: [Histonet] CD68 counts Message-ID: <7722595275A4DD4FA225B92CDBF174A174501D430A@EXC-MBX3.cfs.le.ac.uk> I am attempting to accurately assess the number of CD 68 positive cells (hopefully macrophages) in biopsies, they have been GMA processed, ABC staining, with AEC visualisation; no problem with a nucleated cell with positive cell membrane, but they are in the minority, so the question how does one judge the cut-off point that makes a positive cell into a negative cell if you catch my drift??. I am quite experienced at counting cells but feel that obtaining reliable, consistent results from this material is quite challenging. All ideas/thoughts gratefully received Cheers Richard Edwards University of Leicester U.K. From SARAH.REEVES <@t> ekht.nhs.uk Wed Jan 21 11:58:10 2009 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Wed Jan 21 11:58:34 2009 Subject: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? Message-ID: <49776232020000B500003A02@ekhgwia.ekht.nhs.uk> Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? Thanks in advance Sarah Reeves ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From igor.deyneko <@t> gmail.com Wed Jan 21 12:00:51 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Wed Jan 21 12:00:56 2009 Subject: [Histonet] IHC Background Message-ID: <35e16a770901211000t20dda191hede8ee7b7d5cc46a@mail.gmail.com> Hello everyone! I was wondering if anyone has ever pre-incubated the antibody with normal Mouse IgG to get rid of the unspecific staining. The antibody I use crosreacts with mouse , and both human and mouse tissues stain within a xenograft. I've heard that one can get rid of the mouse staining by incubating with murine IgG. Has anyone ever done that and if so, you mind sharing some info? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From rjbuesa <@t> yahoo.com Wed Jan 21 12:02:30 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 21 12:02:33 2009 Subject: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? In-Reply-To: <49776232020000B500003A02@ekhgwia.ekht.nhs.uk> Message-ID: <166877.92639.qm@web65711.mail.ac4.yahoo.com> The black effect is obtained when you tone with gold chloride. The "golden"?background will remain, it is "part of the deal". Ren? J. --- On Wed, 1/21/09, SARAH REEVES wrote: From: SARAH REEVES Subject: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 21, 2009, 12:58 PM Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? Thanks in advance Sarah Reeves ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hodges420 <@t> msn.com Wed Jan 21 13:12:48 2009 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Wed Jan 21 13:12:52 2009 Subject: [Histonet] decal billing issue Message-ID: Good day all, I have a billing issue and need some input. How do you all bill decals... per container or by block or just by case? Thanks for your input, Tere Hodges St. Mary's Hospital Tucson,Az. _________________________________________________________________ Windows Live? Hotmail??more than just e-mail. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t2_hm_justgotbetter_howitworks_012009 From marktarango <@t> gmail.com Wed Jan 21 13:15:14 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jan 21 13:15:18 2009 Subject: [Histonet] CD68 counts In-Reply-To: <7722595275A4DD4FA225B92CDBF174A174501D430A@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A174501D430A@EXC-MBX3.cfs.le.ac.uk> Message-ID: <5b6eb13e0901211115r3bac3404pc130866ceadf0383@mail.gmail.com> Well, you're going to have dendritic cells staining with CD68. Dendritic cells be really large, full of cytoplasm, and multinucleated. I guess that brings you back to "hopefully macrophages". Maybe someone can recommend a more specific marker or better criteria for what a macrophage is... Mark On Wed, Jan 21, 2009 at 8:48 AM, Edwards, R.E. wrote: > > I am attempting to accurately assess the number of CD 68 positive cells > (hopefully macrophages) in biopsies, they have been GMA processed, ABC > staining, with AEC visualisation; no problem with a nucleated cell with > positive cell membrane, but they are in the minority, so the question > how does one judge the cut-off point that makes a positive cell into a > negative cell if you catch my drift??. I am quite experienced at counting > cells but feel that obtaining reliable, consistent results from this > material is quite challenging. > All ideas/thoughts gratefully received > > Cheers > Richard Edwards > University of Leicester > U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Wed Jan 21 13:18:15 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jan 21 13:18:29 2009 Subject: [Histonet] decal billing issue In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA529A37D@ITSSSXM01V6.one.ads.che.org> Per specimen here... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARY T HODGES Sent: Wednesday, January 21, 2009 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal billing issue Good day all, I have a billing issue and need some input. How do you all bill decals... per container or by block or just by case? Thanks for your input, Tere Hodges St. Mary's Hospital Tucson,Az. _________________________________________________________________ Windows Live(tm) Hotmail(r)...more than just e-mail. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t2_hm_justgotbetter_ howitworks_012009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From HornHV <@t> archildrens.org Wed Jan 21 13:45:50 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jan 21 13:46:31 2009 Subject: [Histonet] decal billing issue In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA529A37D@ITSSSXM01V6.one.ads.che.org> References: <5D64396A0D4A5346BEBC759022AAEAA529A37D@ITSSSXM01V6.one.ads.che.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82FF5@EMAIL.archildrens.org> Same here..by each specimen container... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 3 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 21, 2009 1:18 PM To: MARY T HODGES; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal billing issue Per specimen here... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARY T HODGES Sent: Wednesday, January 21, 2009 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal billing issue Good day all, I have a billing issue and need some input. How do you all bill decals... per container or by block or just by case? Thanks for your input, Tere Hodges St. Mary's Hospital Tucson,Az. _________________________________________________________________ Windows Live(tm) Hotmail(r)...more than just e-mail. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t2_hm_justgotbetter_ howitworks_012009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From lpaveli1 <@t> hurleymc.com Wed Jan 21 14:15:00 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jan 21 14:15:32 2009 Subject: [Histonet] Histobath replacement Message-ID: <49773BF4020000EE00025AFE@smtp-gw.hurleymc.com> Hello all, Thermo has discontinued their snap freezing tissue bath called the Histobath. It uses isopentane (2-methylbutane). Does anyone know of a company that makes a product comparable? thank you From Albert.Santiago <@t> uphs.upenn.edu Wed Jan 21 16:19:35 2009 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Jan 21 16:19:42 2009 Subject: [Histonet] ELISA TESTING certification/training Message-ID: Hello fellow Histonetters, we're in the process of implementing Elisa testing in our lab and I was wondering if anyone knew of any type of certification courses, seminars, training, etc. that our techs can take. Thank you in advance for your help. Have a good day. Albert Santiago, HT (ASCP) Laboratory Supervisor Dermatopathology Phone 215-662-6539 Fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From gentras <@t> vetmed.auburn.edu Wed Jan 21 16:30:54 2009 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jan 21 16:31:01 2009 Subject: [Histonet] Re: tissue processors Message-ID: <4977A21E.2020700@vetmed.auburn.edu> hello, we are in the market for a new/replacement tissue processor. If you have experience and/or pertinent detailed info on any of the following will you please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, and last but not least *3.* VIP 1000 Floor Tissue Processor. Thank you kindly. Atoska From saby_joseph_a <@t> yahoo.com Wed Jan 21 17:51:34 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Jan 21 17:51:42 2009 Subject: [Histonet] Re: tissue processors References: <4977A21E.2020700@vetmed.auburn.edu> Message-ID: <600394.22555.qm@web33808.mail.mud.yahoo.com> I worked with a VIP 1000 well over 20 years ago.? This is a dinosaur! Issues that will come to haunt you: ??? The retort lid will warp over time (if it isn't already warped).? Minor overtensioning of the clamps cause this.? Many years ago we were told that new lids were no longer available. ??? The printed circuit boards failed on a regular basis.? Last I heard, they were no longer made.? Perhaps an aftermarket parts?are now available for these issues.? I remember these units were real work horses.? But they had no where near the bells and whistles you get with more modern units. Good luck! Joe ________________________________ From: Atoska Gentry To: Histonet Sent: Wednesday, January 21, 2009 5:30:54 PM Subject: [Histonet] Re: tissue processors hello, we are in the market for a new/replacement tissue processor. If you have experience and/or pertinent detailed info on any of the following? will you please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, and last but not least? *3.* VIP 1000 Floor Tissue Processor. Thank you kindly. Atoska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Wed Jan 21 18:04:09 2009 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Jan 21 18:04:12 2009 Subject: [Histonet] Auto-IHC Staining Issues Message-ID: <20090122000409.6AD2D5532@alexander.cnchost.com> Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA From mwich <@t> 7thwavelabs.com Wed Jan 21 18:15:35 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Jan 21 18:15:39 2009 Subject: [Histonet] job posting Message-ID: <62A8156F8071C8439080D626DF8C33A65D8B36@wave-mail.7thwave.local> Seventh Wave Laboratories, located 30 miles west of downtown St. Louis, MO is currently seeking a Histotechnologist whose primary functions will be microtomy and staining of histological specimens. Other duties may include tissue trimming, processing, and embedding. Interested candidates may send a resume to: Seventh Wave Laboratories - Human Resources, 743 Spirit 40 Park Drive, Suite 209, Chesterfield, MO 63005 or FAX 636.519.4886 or HR@7thwavelabs.com. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From histoinfo <@t> comcast.net Wed Jan 21 18:41:05 2009 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Wed Jan 21 18:41:11 2009 Subject: [Histonet] double staining on the ventana XT Message-ID: <012220090041.26286.4977C0A1000E014D000066AE221558639401000207019B9C0708@comcast.net> Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer From gvdobbin <@t> ihis.org Wed Jan 21 19:04:57 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Jan 21 19:05:12 2009 Subject: [Histonet] Auto-IHC Staining Issues Message-ID: Hi Paula, You really have to tell us which instrument you are using to get a concise answer. Other details like how the slides are dewaxed for instance would also definately help. Regards, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Paula Lucas 01/21/09 8:04 PM >>> Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From gvdobbin <@t> ihis.org Wed Jan 21 19:08:25 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Jan 21 19:08:56 2009 Subject: [Histonet] double staining on the ventana XT Message-ID: Hi Jennifer, Your Ventana Application Specialist should be more than willing to help you thru this process. I demo'd an XT last year and I recall double-staining was very straight forward. Unfortunately the details of which were not retained by me! Good luck. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> 01/21/09 8:41 PM >>> Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From TMcNemar <@t> lmhealth.org Thu Jan 22 05:04:44 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jan 22 05:05:49 2009 Subject: [Histonet] Xylene substitute Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> Wondering what folks are using as a xylene substitute. We have used Americlear for many years and it has worked well. I would like to find something that is biodegradable and doesn't have such a strong odor. Any suggestions or recommendations? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From abright <@t> brightinstruments.com Thu Jan 22 05:49:53 2009 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Jan 22 05:50:05 2009 Subject: [Histonet] Histobath replacement In-Reply-To: <49773BF4020000EE00025AFE@smtp-gw.hurleymc.com> References: <49773BF4020000EE00025AFE@smtp-gw.hurleymc.com> Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0ACDA2@BRIGHT-SBS.Bright.local> Dear Lynette, Yes we do, in fact our Clini-RF Rapid Freezer goes lower in temperature to -80 deg. C. Plus there are a range of options available. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: 21 January 2009 20:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath replacement Hello all, Thermo has discontinued their snap freezing tissue bath called the Histobath. It uses isopentane (2-methylbutane). Does anyone know of a company that makes a product comparable? thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Jan 22 06:19:55 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 22 06:17:27 2009 Subject: [Histonet] RE: Xylene substitute In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39081F8B74E2@IBMB7Exchange.digestivespecialists.com> Tom, We are using Formula 83 from CBG and like it a lot. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 22, 2009 6:05 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Xylene substitute Wondering what folks are using as a xylene substitute. We have used Americlear for many years and it has worked well. I would like to find something that is biodegradable and doesn't have such a strong odor. Any suggestions or recommendations? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jane.Moose <@t> NewberryHospital.net Thu Jan 22 06:24:45 2009 From: Jane.Moose <@t> NewberryHospital.net (Jane C. Moose) Date: Thu Jan 22 06:26:30 2009 Subject: [Histonet] charges Message-ID: <8BB5FC36DDA373488E60177757BBD69848A33A@ncmhexchbe01.NewberryHospital.net> We are a small hospital lab and are beginning to do some prostate biopsies. There will generally be 12 specimens per case. Do we bill 88305 for each one (12) or is there some kind of "bundling" of charges for these? Thanks for any input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 From rjbuesa <@t> yahoo.com Thu Jan 22 08:28:50 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 22 08:28:58 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> Message-ID: <489142.2699.qm@web65707.mail.ac4.yahoo.com> Tom: Dehydrate with graded?isopropyl alcohol and clear with mixtures 5:1 and 2:1 of isopropyl and mineral oil (at 50?C), followed by pure mineral oil (50?C) and your regular infiltration with paraffin. You will eliminate xylene totally and the used mineral oil will be disposed off mixed with the used paraffin as a solid. If you want I can send you further details and a protocol. Ren? J. --- On Thu, 1/22/09, Tom McNemar wrote: From: Tom McNemar Subject: [Histonet] Xylene substitute To: histonet@pathology.swmed.edu Date: Thursday, January 22, 2009, 6:04 AM Wondering what folks are using as a xylene substitute. We have used Americlear for many years and it has worked well. I would like to find something that is biodegradable and doesn't have such a strong odor. Any suggestions or recommendations? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 22 08:31:21 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 22 08:31:30 2009 Subject: [Histonet] Re: tissue processors In-Reply-To: <4977A21E.2020700@vetmed.auburn.edu> Message-ID: <285071.83688.qm@web65704.mail.ac4.yahoo.com> The VIP 1000 beats all other hands down. Get a demo to be convinced. Ren? J. --- On Wed, 1/21/09, Atoska Gentry wrote: From: Atoska Gentry Subject: [Histonet] Re: tissue processors To: "Histonet" Date: Wednesday, January 21, 2009, 5:30 PM hello, we are in the market for a new/replacement tissue processor. If you have experience and/or pertinent detailed info on any of the following will you please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, and last but not least *3.* VIP 1000 Floor Tissue Processor. Thank you kindly. Atoska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jan 22 08:33:23 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 22 08:33:34 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82FF8@EMAIL.archildrens.org> We use XS xylene substitute and we get it from Statlab. No odor. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 3 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 22, 2009 5:05 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Xylene substitute Wondering what folks are using as a xylene substitute. We have used Americlear for many years and it has worked well. I would like to find something that is biodegradable and doesn't have such a strong odor. Any suggestions or recommendations? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Jan 22 08:33:33 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 22 08:33:45 2009 Subject: [Histonet] Auto-IHC Staining Issues In-Reply-To: <20090122000409.6AD2D5532@alexander.cnchost.com> Message-ID: <506294.70472.qm@web65716.mail.ac4.yahoo.com> Not much help can you get if you do not say which autostainer you are having the problems with, because not all work in the same way. Ren? J. --- On Wed, 1/21/09, Paula Lucas wrote: From: Paula Lucas Subject: [Histonet] Auto-IHC Staining Issues To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 21, 2009, 7:04 PM Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu Jan 22 08:53:54 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 22 08:53:58 2009 Subject: [Histonet] Auto-IHC Staining Issues References: <20090122000409.6AD2D5532@alexander.cnchost.com> Message-ID: <4245DA30730D4E628DF7991A77DEDEE7@auxs.umn.edu> Suggestions: 1) If it's compatible with the autostainer that you're using, add a surfactant such as Tween 20 (0.05%) to your buffer rinse solution, in order to cut the surface tension and allow the staining reagents to flow across your slides more evenly. 2) If it's possible, add more volume of dispensed reagents to each slide. 3) If it won't affect your positive staining intensity, decrease the incubation times in reagents. 4) Make sure there isn't an excess of air flow across your slides (gap in autostainer cover seal). 5) Make sure your autostainer is level. 6) As others have mentioned, make sure that your slides are completely dewaxed. Jan Shivers UMN VDL ----- Original Message ----- From: "Paula Lucas" To: Sent: Wednesday, January 21, 2009 6:04 PM Subject: [Histonet] Auto-IHC Staining Issues Hello, I've been having a problem with the staining quality from an auto-IHC stainer and I was wondering if any of you experience the same thing. I notice sometimes that the staining in the tissue isn't complete, like there are areas where the fluid (one or more of the steps involved) didn't get in contact with the tissue, thus resulting in patchy, skipped staining. I'm thinking that this must be a dispensing problem. Is this a general thing with all auto stainers - ones like DAKO or Lab Vision's stainer or Biocare Medical's stainer? These stainers dispense fluid onto the slide. I hope this explains things and I really hope that I can get some feedback. It's getting very frustrating. Thanks in advance, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Thu Jan 22 08:54:22 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Jan 22 08:54:31 2009 Subject: [Histonet] New Standards Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE801471A58@wlmmsx01.nemours.org> Hello colleagues of the tissue world! I am working on developing (revising, actually) up-dated standards for CLIA proficiency testing in my lab. We are only doing Histochemistry, Immuno fluorescence for dystrophin and dystrophin assoicate proteins and Immunofluorescence for both kidney and cardiac bxs. In lieu of re-inventing the wheel...does anyone already have proficiency standards for any of these, that I might model (...and they might be willing to share), in revising our 10 yr old standards? From NMargaryan <@t> childrensmemorial.org Thu Jan 22 10:22:54 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Jan 22 10:26:31 2009 Subject: [Histonet] keratin 18 Message-ID: Hi histoneters, Please help me to find an Ab to detect keratin 18 in mouse tissue, this means that at has to be not mouse Ab. Thanks in advance, Naira From gu.lang <@t> gmx.at Thu Jan 22 10:51:59 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 22 10:52:11 2009 Subject: AW: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with In-Reply-To: <49776232020000B500003A02@ekhgwia.ekht.nhs.uk> References: <49776232020000B500003A02@ekhgwia.ekht.nhs.uk> Message-ID: Do you make the silversolution with ammonia? If the silversolution isn't properly retitered with silvernitrat, there is an excess amount of ammonia, that makes a yellowish background. The silversolution has to be faintly grey. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von SARAH REEVES Gesendet: Mittwoch, 21. J?nner 2009 18:58 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a go Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain? Thanks in advance Sarah Reeves ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jan 22 10:56:55 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 22 10:57:06 2009 Subject: AW: [Histonet] double staining on the ventana XT In-Reply-To: <012220090041.26286.4977C0A1000E014D000066AE221558639401000207019B9C0708@comcast.net> References: <012220090041.26286.4977C0A1000E014D000066AE221558639401000207019B9C0708@comcast.net> Message-ID: <3AA9BCB5A63B4A5D89DC59CD2F7BDF89@dielangs.at> Hi Jennifer, we perform the doublestain routinly. The preferred protocol is CK HMW/p504s on prostata biopsies. The protocol is easy to establish and the staining results are very good. The run lasts about 4 hours and we do it overnight. You need the Red and the DAB ultraview kit. No extra reagens. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 22. J?nner 2009 01:41 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] double staining on the ventana XT Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer From nancy_schmitt <@t> pa-ucl.com Thu Jan 22 10:25:45 2009 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jan 22 11:01:35 2009 Subject: [Histonet] stock solutions Message-ID: <71320B4EBC7C15419563EAFBFCD924651EDF7179E1@hades.pa-ucl.com> Help! We have a student in the histo program. She is making stock solution for silver stain and Giemsa stain. How long are stock solutions good for? We are not finding that info..... Thanks Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Jennifer.Bull <@t> northwestpathology.com Thu Jan 22 11:18:37 2009 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Jan 22 11:18:43 2009 Subject: [Histonet] Auto-IHC Staining Issues Message-ID: <85760CECEC18444BB95F26D5E88DAEAA21FC110C6B@hinet2.hinet.org> Paula- It's a hard problem to diagnose with out knowing what platform you are using, but here is what I have learned from our experience- - We had the same trouble on our Ventana Benchmark and after much hair pulling and troubleshooting, found it to be the slides we were using. The only slides that Ventana recommends for use on their stainer are the Erie superfrost. This fixed the problem for us. We have since found that the Bond360 slides from TruScientific work equally as well. - We also found that we had similar problems on Dako's Autostainer. This time it was due to the instrument not being completely level. We also learned that when transferring the slides to buffer after the retrieval process, you need to be quick or they can dry out, causing artifact or patchy staining effect. This too was easily solved by using a tween buffer or by using different racks to transfer the slides. I hope this is of help to you, I know how frustrating troubleshooting staining issues can be! Jennifer Bull jennifer.bull@nwpathology.com From Farnana <@t> nehealth.com Thu Jan 22 11:21:56 2009 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Thu Jan 22 11:22:02 2009 Subject: [Histonet] job opening Message-ID: <497864E4.26ED.00D9.0@nehealth.com> Albany Memorial Hospital Albany, NY Full time Histotechnician position available 6:30 am to 2:30 pm, Mon - Friday, no weekends. Must be NYS licensed and have ASCP certification. Responsibilities include, routine histology, IHC, ISH, special stains and frozen sectioning. If interested please submit a resume to: Amy Farnan Histology Supervisor Farnana@nehealth.com Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From froyer <@t> bitstream.net Thu Jan 22 11:36:57 2009 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Jan 22 11:37:05 2009 Subject: [Histonet] Tissue Processors (VIP-1000) In-Reply-To: References: Message-ID: <8AE466168B8A4859948D5013C53ED194@Ford> I have been servicing the VIP-1000/2000/3000 (aka: "K" Series) tissue processors for over 15 years. They were manufactured new from approximately 1983 to 1993. They were manufactured and private labeled for Miles Scientific, Inc. by Sakura Finetek. In the time Miles sold them new, I estimate that well over 10k units were placed. In the 15+ years that I have serviced them, I have rarely come across the problems that Joe describes. It is true that the Retort Lid can become warped over time, and that brand new replacement parts are no longer available from Sakura, but there are so many units in the field, and many refurbished equipment companies with their own used parts departments, that a used replacement lid (that is not warped) is easily found. As to the electronics package that Joe mentions, again, of the hundreds that I have serviced over the years and to this very day, I have never had an electronics package (PCB/CPU) fail. It is true that the Power Supply to the electronics package does have a limited life span and will burn out over time (this may be what Joe experienced). But the Power Supply is a very common component and brand new replacement units are readily available from the electronics supply market. I am not saying that Joe did not experience a failure of one specific solid state PCB/CPU... it can happen. But it is very rare and does not reflect the continued performance of the thousands of units that are out there... either still in continuous use from the original date of purchase, or serving a second life as a refurbished unit. If you would like further details of my experience with the "K" Series VIP tissue Processor, please contact me off-List. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com From jrobertson <@t> pathologysciences.com Thu Jan 22 11:39:25 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Thu Jan 22 11:39:34 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E094@lmhsmail.lmhealth.org> Message-ID: <518CD6920AA7154193CBE5977CD88073177E4A@psmgsrv2.PSMG.local> We use both Propar and Formula 83. Propar works well for the most part but does have a tendency if it's humid, to dry moisture out of the air as we have seen eosin bleed from time to time with it. It doesn't have much of an odor either. Formula 83 works wonderfully but it smells like ether! We use this on our processors as it distills really well and doesn't have the tendency to draw moisture. Hope this information helps. Jodie Robertson, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 22, 2009 3:05 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Xylene substitute Wondering what folks are using as a xylene substitute. We have used Americlear for many years and it has worked well. I would like to find something that is biodegradable and doesn't have such a strong odor. Any suggestions or recommendations? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jan 22 12:22:42 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 22 12:22:55 2009 Subject: AW: [Histonet] double staining on the ventana XT In-Reply-To: <397958B23C3E4D43AB50099AE6313C3804FB59BB@slhmailsvr.slhn.org> References: <3AA9BCB5A63B4A5D89DC59CD2F7BDF89@dielangs.at> <397958B23C3E4D43AB50099AE6313C3804FB59BB@slhmailsvr.slhn.org> Message-ID: <387080610357474EA9BC4BAB46372F37@dielangs.at> Kim, We also use the Basalcellcocktail (with p63). The protocol is put together of the two single protocols for the single markers with the usual incubation times. The first HIER step is naturally the retrieval for both antigens. The first part is the dab-kit. After the colour-development a second HIER step takes place. You can choose time and temperature (eg. 60 degrees, 15 min; or 90 degrees 8 min). That depends on how easily your first primary and secondary antibodies can be denatured. Than the Red-kit follows. At the end is counterstain and blueing. If the first primary and sec. ab are'nt denatured you get an overall red result. If the second HIER step is too harsh, you see it in the morphology or high background. Gudrun -----Urspr?ngliche Nachricht----- Von: Artim, Kimberly [mailto:ArtimK@slhn.org] Gesendet: Donnerstag, 22. J?nner 2009 18:02 An: gu.lang@gmx.at Betreff: RE: [Histonet] double staining on the ventana XT Guadrun, Would you be able to provide me more detailed protocol information for the prostate biopsy staining. Are you using P63 as well? Any information you provide would be greatly appreciated. Thank you, Kim Kimberly Artim, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital & Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, January 22, 2009 11:57 AM To: histoinfo@comcast.net Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] double staining on the ventana XT Hi Jennifer, we perform the doublestain routinly. The preferred protocol is CK HMW/p504s on prostata biopsies. The protocol is easy to establish and the staining results are very good. The run lasts about 4 hours and we do it overnight. You need the Red and the DAB ultraview kit. No extra reagens. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 22. J?nner 2009 01:41 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] double staining on the ventana XT Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From algranth <@t> u.arizona.edu Thu Jan 22 12:32:30 2009 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Jan 22 12:31:53 2009 Subject: [Histonet] stock solutions In-Reply-To: <71320B4EBC7C15419563EAFBFCD924651EDF7179E1@hades.pa-ucl.co m> References: <71320B4EBC7C15419563EAFBFCD924651EDF7179E1@hades.pa-ucl.com> Message-ID: <6.2.3.4.1.20090122112849.027091a0@algranth.inbox.email.arizona.edu> Look in Freida Carson's second edition, page 80. Stock Giemsa is one yr Stock silver nitrate for GMS is one month Andi Grantham At 09:25 AM 1/22/2009, Nancy Schmitt wrote: >Help! >We have a student in the histo program. She is making stock >solution for silver stain and Giemsa stain. How long are stock >solutions good for? We are not finding that info..... >Thanks >Nancy Schmitt >Dubuque, IA > >NOTICE: This email may contain legally privileged information. The >information is for the use of only the intended recipient(s) even if >addressed incorrectly. If you are not the intended recipient, please >notify the sender that you have received it in error and then delete >it along with any attachments. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From RCazares <@t> schosp.org Thu Jan 22 12:59:08 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Jan 22 12:59:56 2009 Subject: [Histonet] Prostate needle saturation biopsies Message-ID: <0672286797B07E40AA3414F534B7CB800400DBBE14@EXCHCCRMB.schosp.org> Hello Histonetters, With the new Medicare G codes for saturation biopsies of the prostate, the flyer reads: G0416, Surgical pathology gross and microscopic exam for prostate needle saturation biopsy sampling, 1 to 20 specimens. G0417, 21 to 40 specimens. G0418, 41 to 60 specimens. G0419, greater than 60 specimens. We seem to be having a difference of opinion as to how we should use these new codes. Some of us think that if we get forty cores both G0416 and G0417 should be used. Some think only G0417 should be used and some just don't know. Medicare was no help, they said "Write a letter". How are others out there using these codes? Ruth Cazares Histology Supervisor Department of Pathology Swedish Covenant Hospital *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From LRaff <@t> uropartners.com Thu Jan 22 13:06:11 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Jan 22 13:06:23 2009 Subject: [Histonet] Prostate needle saturation biopsies In-Reply-To: <0672286797B07E40AA3414F534B7CB800400DBBE14@EXCHCCRMB.schosp.org> References: <0672286797B07E40AA3414F534B7CB800400DBBE14@EXCHCCRMB.schosp.org> Message-ID: We participated in the CAP teleconference in December. The CAP recommendation is to use 1 of the G codes per case and that it does not matter how many jars are submitted, just how many cores. (Yes, this is different than the standard 1 jar/ 1 billing code policy). Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Thursday, January 22, 2009 12:59 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate needle saturation biopsies Hello Histonetters, With the new Medicare G codes for saturation biopsies of the prostate, the flyer reads: G0416, Surgical pathology gross and microscopic exam for prostate needle saturation biopsy sampling, 1 to 20 specimens. G0417, 21 to 40 specimens. G0418, 41 to 60 specimens. G0419, greater than 60 specimens. We seem to be having a difference of opinion as to how we should use these new codes. Some of us think that if we get forty cores both G0416 and G0417 should be used. Some think only G0417 should be used and some just don't know. Medicare was no help, they said "Write a letter". How are others out there using these codes? Ruth Cazares Histology Supervisor Department of Pathology Swedish Covenant Hospital *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> exchange.hsc.mb.ca Thu Jan 22 13:21:25 2009 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Thu Jan 22 13:21:37 2009 Subject: [Histonet] RE: Prion Contamination Message-ID: Hi, I was reading your email of Jan. 15/09 on the subject of "Prion Contamination". In it you had made the following statement, which I was curious as to where this information had come from. "the procedure to inactivate using formic acid is followed before fixation and processing. If the tissue is fixed (formalin or other common fixatives) then you would be actually "fixing" the prion's ability to NOT be inactivated". I had read again the WHO Guidelines on CJD that you had a link to & could find no mention of the fact that the formic acid step for de-activating the prions had to be done before any fixation. The WHO instructions: P. 18 8.2.2 Histopathological examination: states "formic acid treatment consists of placing small pieces of fixed tissue, no more than 4 to 5 mm thick, in 50 to 100 ml of 95% formic acid for an hour". I have been dealing with CJD brains for many years always following the CDC, WHO & Health Canada guidelines but have never read any studies that had indicated that the fixing in formalin made the treatment in formic acid ineffective. If you have any further information I would really appreciate seeing it. Thank you S. Allen sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From nsnwl <@t> neuro.hfh.edu Thu Jan 22 13:56:23 2009 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Thu Jan 22 13:56:33 2009 Subject: [Histonet] Starfrost slides Message-ID: <9ccd9b8d2f54347c4c943545533fe923@neuro.hfh.edu> Has anyone using Starfrost slides (7255) from Mercedes had any problems with autofluoresence when doing IF? Thanks in advance. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From gu.lang <@t> gmx.at Thu Jan 22 15:08:44 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 22 15:08:53 2009 Subject: AW: [Histonet] Starfrost slides In-Reply-To: <9ccd9b8d2f54347c4c943545533fe923@neuro.hfh.edu> References: <9ccd9b8d2f54347c4c943545533fe923@neuro.hfh.edu> Message-ID: <86D39AB2A3074D60A6B771432C6E26E1@dielangs.at> One time we tried FISH on a cellsuspension dropped on a superfrost slide. It was airdried. In the fluorescence microscope the nuclei were surrounded with strong fluorescence. In another trial we heated the slides before FISH and the slide was more or less clear. I concluded, that the reagens on the slide surface must be altered through the heat. Just a single experience. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Nancy Lemke Gesendet: Donnerstag, 22. J?nner 2009 20:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Starfrost slides Has anyone using Starfrost slides (7255) from Mercedes had any problems with autofluoresence when doing IF? Thanks in advance. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit ============================================================================ == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Jan 22 17:46:19 2009 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 22 17:46:24 2009 Subject: [Histonet] RE: Prion Contamination In-Reply-To: References: Message-ID: I just researched this recently for procedure writing. I referenced an article from the CDC that made mention of specific handling guidelines for histology, and also specifically the formic acid treatment step. I do not have the article in front of me, but by my recollection I believe that it stated that the formic acid was a post-fixation step. I found this article relatively easily by using the search field for CJD from the CDC website. You can check my recollection here: http://www.cdc.gov/od/ohs/biosfty/bmbl5/sections/SectionVIIIH-PrionDiseases.pdf Thanks Joelle Weaver HTL(ASCP) > Date: Thu, 22 Jan 2009 13:21:25 -0600> From: SAllen@exchange.hsc.mb.ca> To: tbraud@holyredeemer.com> Subject: [Histonet] RE: Prion Contamination> CC: histonet@lists.utsouthwestern.edu> > Hi,> I was reading your email of Jan. 15/09 on the subject of "Prion> Contamination". In it you had made the following statement, which I was> curious as to where this information had come from. > "the procedure to inactivate using formic acid is followed before> fixation and processing. If the tissue is fixed (formalin or other> common fixatives) then you would be actually "fixing" the prion's> ability to NOT be inactivated". > I had read again the WHO Guidelines on CJD that you had a link to &> could find no mention of the fact that the formic acid step for> de-activating the prions had to be done before any fixation. The WHO> instructions: P. 18 8.2.2 Histopathological examination: states "formic> acid treatment consists of placing small pieces of fixed tissue, no more> than 4 to 5 mm thick, in 50 to 100 ml of 95% formic acid for an hour". I> have been dealing with CJD brains for many years always following the> CDC, WHO & Health Canada guidelines but have never read any studies that> had indicated that the fixing in formalin made the treatment in formic> acid ineffective. If you have any further information I would really> appreciate seeing it. > Thank you > S. Allen> sallen@hsc.mb.ca> > _________________________________________________________________ Windows Live?: E-mail. Chat. Share. Get more ways to connect. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_allup_explore_012009 From saby_joseph_a <@t> yahoo.com Thu Jan 22 17:47:15 2009 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Thu Jan 22 17:47:19 2009 Subject: [Histonet] Tissue Processors (VIP-1000) References: <8AE466168B8A4859948D5013C53ED194@Ford> Message-ID: <970172.98586.qm@web33804.mail.mud.yahoo.com> All- I did say the unit was a real workhorse!? In all honesty, we did use ours hard for well over 10 years before we moved on the the newer VIP 2000s and VIP 3000s. Unfortunately, we remember our last experiences better than the early ones.? We had many good years with our VIP 1000s.? It wasn't until the end that we had problems.? Perhaps our service rep wasn't very good at scouting out parts (something I suspected very strongly at the time).? Perhaps he was smelling the commission on a new processor.? All of?the later?VIPs come with?my very strong recommendation.? Very solid equipment, very dependable.? Very, very few interrupted runs, and most of these were operator error.? If Ford Royer says he can get parts, then I would certainly recommend the VIP 1000 as well.? Again, years ago I was told parts were unavailable. But, again, I think that was just the person servicing my unit. Jos Saby ________________________________ From: Ford Royer To: histonet Sent: Thursday, January 22, 2009 12:36:57 PM Subject: RE: [Histonet] Tissue Processors (VIP-1000) I have been servicing the VIP-1000/2000/3000 (aka: "K" Series) tissue processors for over 15 years.? They were manufactured new from approximately 1983 to 1993.? They were manufactured and private labeled for Miles Scientific, Inc. by Sakura Finetek. In the time Miles sold them new, I estimate that well over 10k units were placed.? In the 15+ years that I have serviced them, I have rarely come across the problems that Joe describes. It is true that the Retort Lid can become warped over time, and that brand new replacement parts are no longer available from Sakura, but there are so many units in the field, and many refurbished equipment companies with their own used parts departments, that a used replacement lid (that is not warped) is easily found.? As to the electronics package that Joe mentions, again, of the hundreds that I have serviced over the years and to this very day, I have never had an electronics package (PCB/CPU) fail.? It is true that the Power Supply to the electronics package does have a limited life span and will burn out over time (this may be what Joe experienced).? But the Power Supply is a very common component and brand new replacement units are readily available from the electronics supply market.? I am not saying that Joe did not experience a failure of one specific solid state PCB/CPU... it can happen.? But it is very rare and does not reflect the continued performance of the thousands of units that are out there... either still in continuous use from the original date of purchase, or serving a second life as a refurbished unit. If you would like further details of my experience with the "K" Series VIP tissue Processor, please contact me off-List. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 Web: http://www.minnesotamedical.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Jan 22 20:16:22 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Jan 22 20:16:30 2009 Subject: [Histonet] Re: Xylene substitute Message-ID: Questions about xylene substitutes: This has been discussed a good many times on Histonet. There are two classes of xylene substitutes: Limonene and turpentine are terpenes. Several brands; AmeriClear was mentioned. Limonene is prepared by steam distillation of orange peels. It has a strong citrus smell variously described as pleasant, overwhelming, disgusting, and allergenic, and cannot be made odorless. It is not very toxic, is not easily set afire, and is to a degree biodegradable. It cannot be distilled. With America's declining citrus industry, this once cheap product has become considerably more expensive. Aliphatic solvents are now in much more widespread use. They are more expensive than xylene, but can be recovered by distillation. Most of them are odorless, at least to my nose. They are not very toxic. Some have much lower flash points than others, so that fire hazard varies considerably. They are not easiy biodegradable. Different brands differ considerably in chemical and physical properties, and distillation routines for one brand cannot be used with another brand. Richard Allan's Clear-Rite 3 may be the most widely used aliphatic. ANATECH's Pro-Par was mentioned in this correspondence, and is very meticulously described on their Web site. I hadn't heard of CBG Biotech's Formula 83 before. Slightly different from ordinary aliphatics, it is described as a "naphthenic hydrocarbon blend" (cycloalkane). It is described as "smells like ether", and I would be concerned about its very low flash point (45 F, below room temperature, as described in the MSDS). When you mention who makes a commercial product, please tell us who makes it, and read the Materials Safety Data Sheet (MSDS) at the product's Web site and see what you can find out what's in it. I have no commercial connection with any product I've mentioned. Bob Richmond Samurai Pathologist Knoxville TN From pieronelva01 <@t> bigpond.com Fri Jan 23 02:17:50 2009 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Jan 23 02:18:00 2009 Subject: [Histonet] double staining on the ventana XT References: <012220090041.26286.4977C0A1000E014D000066AE221558639401000207019B9C0708@comcast.net> <3AA9BCB5A63B4A5D89DC59CD2F7BDF89@dielangs.at> Message-ID: <9C36B46FD1904F998E18DD8280AA855F@pentium4> We also run a p63/HMCK/p504s dual stain cocktail on prostate cores. Easy to set up and results look great. THe Kappa/Lambda dual stain works, too. I find the red chromagen (lambda) in this setting is a little less precise, tho'. REgards Piero Nelva Anatomical PAthology Monash Medical Centre ----- Original Message ----- From: "Gudrun Lang" To: Cc: Sent: Friday, January 23, 2009 3:56 AM Subject: AW: [Histonet] double staining on the ventana XT Hi Jennifer, we perform the doublestain routinly. The preferred protocol is CK HMW/p504s on prostata biopsies. The protocol is easy to establish and the staining results are very good. The run lasts about 4 hours and we do it overnight. You need the Red and the DAB ultraview kit. No extra reagens. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 22. J?nner 2009 01:41 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] double staining on the ventana XT Greetings, We are looking into doing some immuno double staining. I searched the archives and found a few similar inquiries but no responses. Anyone with experiences to share I would love to hear what you have to say. Everything from what not to do to what works well for you. Many Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.176 / Virus Database: 270.10.12/1910 - Release Date: 1/22/2009 6:28 PM From jqb7 <@t> cdc.gov Fri Jan 23 04:48:58 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jan 23 04:50:04 2009 Subject: [Histonet] RE: Prion Contamination In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70402AE45@LTA3VS011.ees.hhs.gov> All, The formic acid is a post-fixation step. http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s7d.htm#Table%205.%20Tissue %20preparation Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, January 22, 2009 6:46 PM To: sallen@exchange.hsc.mb.ca; Histonet Subject: RE: [Histonet] RE: Prion Contamination I just researched this recently for procedure writing. I referenced an article from the CDC that made mention of specific handling guidelines for histology, and also specifically the formic acid treatment step. I do not have the article in front of me, but by my recollection I believe that it stated that the formic acid was a post-fixation step. I found this article relatively easily by using the search field for CJD from the CDC website. You can check my recollection here: http://www.cdc.gov/od/ohs/biosfty/bmbl5/sections/SectionVIIIH-PrionDisea ses.pdf Thanks Joelle Weaver HTL(ASCP) > Date: Thu, 22 Jan 2009 13:21:25 -0600> From: > SAllen@exchange.hsc.mb.ca> To: tbraud@holyredeemer.com> Subject: > [Histonet] RE: Prion Contamination> CC: > histonet@lists.utsouthwestern.edu> > Hi,> I was reading your email of > Jan. 15/09 on the subject of "Prion> Contamination". In it you had > made the following statement, which I was> curious as to where this > information had come from. > "the procedure to inactivate using formic > acid is followed before> fixation and processing. If the tissue is > fixed (formalin or other> common fixatives) then you would be actually > "fixing" the prion's> ability to NOT be inactivated". > I had read > again the WHO Guidelines on CJD that you had a link to &> could find > no mention of the fact that the formic acid step for> de-activating > the prions had to be done before any fixation. The WHO> instructions: > P. 18 8.2.2 Histopathological examination: states "formic> acid > treatment consists of placing small pieces of fixed tissue, no more> > than 4 to 5 mm thick, in 50 to 100 ml of 95% formic acid for an hour". > I> have been dealing with CJD brains for many years always following > the> CDC, WHO & Health Canada guidelines but have never read any > studies that> had indicated that the fixing in formalin made the > treatment in formic> acid ineffective. If you have any further > information I would really> appreciate seeing it. > Thank you > S. > Allen> sallen@hsc.mb.ca> > _________________________________________________________________ Windows Live(tm): E-mail. Chat. Share. Get more ways to connect. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_allup_explore_012009 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Jan 23 06:32:05 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jan 23 06:29:39 2009 Subject: [Histonet] Re: Xylene substitute In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39081F8B74EE@IBMB7Exchange.digestivespecialists.com> Dear Bob Richmond, our honorable Samurai Pathologist. The consensus of opinion around here is that you have been spending too much time smelling formalin! We can't figure out how you get the smell of ether from Formula 83! Most of us here are old enough to remember the smell of ether but don't equate it with Formula 83. Respectfully, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, January 22, 2009 9:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitute Questions about xylene substitutes: This has been discussed a good many times on Histonet. There are two classes of xylene substitutes: Limonene and turpentine are terpenes. Several brands; AmeriClear was mentioned. Limonene is prepared by steam distillation of orange peels. It has a strong citrus smell variously described as pleasant, overwhelming, disgusting, and allergenic, and cannot be made odorless. It is not very toxic, is not easily set afire, and is to a degree biodegradable. It cannot be distilled. With America's declining citrus industry, this once cheap product has become considerably more expensive. Aliphatic solvents are now in much more widespread use. They are more expensive than xylene, but can be recovered by distillation. Most of them are odorless, at least to my nose. They are not very toxic. Some have much lower flash points than others, so that fire hazard varies considerably. They are not easiy biodegradable. Different brands differ considerably in chemical and physical properties, and distillation routines for one brand cannot be used with another brand. Richard Allan's Clear-Rite 3 may be the most widely used aliphatic. ANATECH's Pro-Par was mentioned in this correspondence, and is very meticulously described on their Web site. I hadn't heard of CBG Biotech's Formula 83 before. Slightly different from ordinary aliphatics, it is described as a "naphthenic hydrocarbon blend" (cycloalkane). It is described as "smells like ether", and I would be concerned about its very low flash point (45 F, below room temperature, as described in the MSDS). When you mention who makes a commercial product, please tell us who makes it, and read the Materials Safety Data Sheet (MSDS) at the product's Web site and see what you can find out what's in it. I have no commercial connection with any product I've mentioned. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Fri Jan 23 08:40:11 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Jan 23 08:40:27 2009 Subject: [Histonet] Cytology Autostainers Message-ID: Hi Folks, What's out there? What's hot and what's not? Anyone using their cytology autostainer for histology special stains? Welcome all input (including vendors). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From relia1 <@t> earthlink.net Fri Jan 23 09:16:03 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Jan 23 09:16:08 2009 Subject: [Histonet] RELIA Special Job Alert Brand New Management and Supervisory Opportunities 1-23-09 Message-ID: Hi Histonetters, I have several brand new exciting opportunities for experienced Managers,and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. Here are my BRAND NEW JOBS: Histology Supervisor Night Shift ? Ft. Lauderdale, FL Histology Supervisor ? Atlanta, GA Here are some of my other leadership positions: Histology Manager ? Maryland, West of DC Research Histology Manager ? Boston, MA Histology Supervisor - Los Angeles, CA If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam New for 2009 RELIA is offering a $500 referral bonus for anyone you refer and I place and a $500 hiring bonus if I place you! There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From victor <@t> pathology.washington.edu Fri Jan 23 09:19:15 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Jan 23 09:19:14 2009 Subject: [Histonet] Re: Xylene substitute In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39081F8B74EE@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39081F8B74EE@IBMB7Exchange.digestivespecialists.com> Message-ID: <4979DFF3.8070201@pathology.washington.edu> In defense of the good Samurai, he was using a comment posted by someone else that the product smelled like ether. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Blazek, Linda wrote: > Dear Bob Richmond, our honorable Samurai Pathologist. The consensus of opinion around here is that you have been spending too much time smelling formalin! We can't figure out how you get the smell of ether from Formula 83! Most of us here are old enough to remember the smell of ether but don't equate it with Formula 83. > > Respectfully, > Linda > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Thursday, January 22, 2009 9:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Xylene substitute > > Questions about xylene substitutes: This has been discussed a good > many times on Histonet. > > There are two classes of xylene substitutes: > > Limonene and turpentine are terpenes. Several brands; AmeriClear was > mentioned. Limonene is prepared by steam distillation of orange peels. > It has a strong citrus smell variously described as pleasant, > overwhelming, disgusting, and allergenic, and cannot be made odorless. > It is not very toxic, is not easily set afire, and is to a degree > biodegradable. It cannot be distilled. With America's declining citrus > industry, this once cheap product has become considerably more > expensive. > > Aliphatic solvents are now in much more widespread use. They are more > expensive than xylene, but can be recovered by distillation. Most of > them are odorless, at least to my nose. They are not very toxic. Some > have much lower flash points than others, so that fire hazard varies > considerably. They are not easiy biodegradable. Different brands > differ considerably in chemical and physical properties, and > distillation routines for one brand cannot be used with another brand. > > Richard Allan's Clear-Rite 3 may be the most widely used aliphatic. > ANATECH's Pro-Par was mentioned in this correspondence, and is very > meticulously described on their Web site. > > I hadn't heard of CBG Biotech's Formula 83 before. Slightly different > from ordinary aliphatics, it is described as a "naphthenic hydrocarbon > blend" (cycloalkane). It is described as "smells like ether", and I > would be concerned about its very low flash point (45 F, below room > temperature, as described in the MSDS). > > When you mention who makes a commercial product, please tell us who > makes it, and read the Materials Safety Data Sheet (MSDS) at the > product's Web site and see what you can find out what's in it. > > I have no commercial connection with any product I've mentioned. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jessgrocki <@t> yahoo.com Fri Jan 23 11:38:33 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Fri Jan 23 11:38:37 2009 Subject: [Histonet] Legal Question Message-ID: <722745.33700.qm@web82001.mail.mud.yahoo.com> Hi, We have an antibody that we ordered in error. We would like to see if another lab would like to purchase it from us. Is that legal to ask this on the Histonet? Thanks! ? Jessica Piche-Grocki, HT(ASCP) From Jackie.O'Connor <@t> abbott.com Fri Jan 23 11:50:31 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jan 23 11:51:03 2009 Subject: [Histonet] Legal Question In-Reply-To: <722745.33700.qm@web82001.mail.mud.yahoo.com> Message-ID: Or you can just ask the supplier to take it back. Jessica Piche Sent by: histonet-bounces@lists.utsouthwestern.edu 01/23/2009 11:38 AM To histonet cc Subject [Histonet] Legal Question Hi, We have an antibody that we ordered in error. We would like to see if another lab would like to purchase it from us. Is that legal to ask this on the Histonet? Thanks! Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Fri Jan 23 12:32:12 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Jan 23 12:32:45 2009 Subject: [Histonet] Legal Question Message-ID: <4979C6DC020000EE00025C4A@smtp-gw.hurleymc.com> This happened to me once (and believe you me; only once cured me). We order through our general services department using an online program. I wanted 1 antibody. I typed 11. General services didn't question it. Then when all eleven arrived to my horror, I called the company, and they refused my request to return them. You can imagine what that bill was!! Yep, that was a Corona day. Sorry, don't know if there are any legal issues involved, but I'm sure you could work something out between other labs in your area. I did. Especially if you know them. At least you didn't order eleven!!! :) >>> Jackie M O'Connor 01/23/09 12:50 PM >>> Or you can just ask the supplier to take it back. Jessica Piche Sent by: histonet-bounces@lists.utsouthwestern.edu 01/23/2009 11:38 AM To histonet cc Subject [Histonet] Legal Question Hi, We have an antibody that we ordered in error. We would like to see if another lab would like to purchase it from us. Is that legal to ask this on the Histonet? Thanks! Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hodges420 <@t> msn.com Fri Jan 23 13:16:44 2009 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Jan 23 13:16:48 2009 Subject: [Histonet] (no subject) Message-ID: Good day all, Thank you so much for your input on decal billing. I have another questain? This one is a little strange. When you put in charges as a prelimary charge ... do you over bill or under bill? I have always stayed under while putting in charges and then audit the doctors charges the next day. example colon segment other then tumor... bill 88307 the next day tumor was found now it is bumped up to 88309 Thanks again for all your help. Tere Hodges St. Mary's Hospital Tucson, Az. _________________________________________________________________ Windows Live? Hotmail?:?more than just e-mail. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_hm_justgotbetter_explore_012009 From leiker <@t> buffalo.edu Fri Jan 23 14:00:14 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Jan 23 14:00:22 2009 Subject: [Histonet] Starfrost slides In-Reply-To: <9ccd9b8d2f54347c4c943545533fe923@neuro.hfh.edu> References: <9ccd9b8d2f54347c4c943545533fe923@neuro.hfh.edu> Message-ID: <6EA8542F07CF9F9D9D69BD53@bchwxp2702.ad.med.buffalo.edu> I have used them almost daily for a year or more now and have had no problems with the slides giving off any autofluorescence in immunofluorescence applications. --On Thursday, January 22, 2009 2:56 PM -0500 Nancy Lemke wrote: > Has anyone using Starfrost slides (7255) from Mercedes had any problems > with autofluoresence when doing IF? Thanks in advance. > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > > > ========================================================================= > ===== CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or > otherwise protected from disclosure. This email is intended for use only > by the person or entity to whom it is addressed. If you are not the > intended recipient, any use, disclosure, copying, distribution, printing, > or any action taken in reliance on the contents of this email, is > strictly prohibited. If you received this email in error, please contact > the sending party by reply email, delete the email from your computer > system and shred any paper copies. > Note to Patients: There are a number of risks you should consider before > using e-mail to communicate with us. See our Privacy Policy and Henry > Ford My Health at www.henryford.com for more detailed information. If you > do not believe that our policy gives you the privacy and security > protection you need, do not send e-mail or Internet communications to us. > > ========================================================================= > ===== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From jennifer.harvey <@t> vanderbilt.edu Fri Jan 23 14:12:45 2009 From: jennifer.harvey <@t> vanderbilt.edu (Jennifer Harvey) Date: Fri Jan 23 14:12:55 2009 Subject: [Histonet] Starfrost slides In-Reply-To: <9ccd9b8d2f54347c4c943545533fe923@neuro.hfh.edu> Message-ID: >From what I understand they are made with green glass. Green glass is a less superior glass than white and you can have fluorescence issues. You should only use white glass for fluorescence applications. You can tell by looking at the slides in the box on their sides. The Mercedes slides (7255) are fine for H&E and regular immuno. I use them for one of my researchers to help with his costs and have been quite happy with them. I do say that I do use the coverslips (mer2450) and they are to be made of green glass also. I am not sure of this since when you look at them on there side in the box they don't seem green. I have used them on fluorescent work and haven't noticed a problem. I hope this helps. I had the same question when I was trying to save money on slide costs. > Jennifer Harvey, HT(ASCP) QIHC > Vanderbilt Vision Research Center > Core Histologist > RM 8105 MCE North Tower > 1215 21st Ave. South > Nashville, TN 37232-8808 > Phone 615-936-1486 > On 1/22/09 1:56 PM, "Nancy Lemke" wrote: > Has anyone using Starfrost slides (7255) from Mercedes had any problems with > autofluoresence when doing IF? > Thanks in advance. > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > > > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the sender that > may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected > from disclosure. This email is intended for use only by the person or entity > to whom it is addressed. If you are not the intended recipient, any use, > disclosure, copying, distribution, printing, or any action taken in reliance > on the contents of this email, is strictly prohibited. If you received this > email in error, please contact the sending party by reply email, delete the > email from your computer system and shred any paper copies. > > Note to Patients: There are a number of risks you should consider before using > e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health > at www.henryford.com for more detailed information. If you do not believe that > our policy gives you the privacy and security protection you need, do not send > e-mail or Internet communications to us. > > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Jan 23 14:13:36 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jan 23 14:13:40 2009 Subject: [Histonet] LabVision/Dako Autostainer In-Reply-To: <722745.33700.qm@web82001.mail.mud.yahoo.com> References: <722745.33700.qm@web82001.mail.mud.yahoo.com> Message-ID: We have earlier generation Dako Autostainers and are evaluating a current generation LabVision stainer. New or old we have experienced occasions when this instrument design has failed to dispense reagent. This seems to occur randomly or if there is a pattern we haven't figured it out. I've recently learned that some owners of the Biocare Nemesis which is essentially the same instrument have also encountered this problem. The arm will travel to the correct slide but nothing dispenses from the probe, then the arm moves on its way. I'd appreciate learning if others have experienced this problem and if a solution to the problem was discovered I hope you'll share it with me. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 From Cindy.J.Chard-Bergstrom <@t> aphis.usda.gov Fri Jan 23 14:32:08 2009 From: Cindy.J.Chard-Bergstrom <@t> aphis.usda.gov (Cindy.J.Chard-Bergstrom@aphis.usda.gov) Date: Fri Jan 23 14:32:15 2009 Subject: [Histonet] Ventana Basic V-Red Kit Message-ID: How many labs out there are still using the basic V-Red Kit from Ventana? Have you had any problems using the enhanced or the ultraview red on animal tissues? Cindy From christiegowan <@t> msn.com Fri Jan 23 14:50:08 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Jan 23 14:51:34 2009 Subject: [Histonet] LabVision/Dako Autostainer In-Reply-To: References: <722745.33700.qm@web82001.mail.mud.yahoo.com> Message-ID: Hey Vinnie, We have 2 lab visions and we also experienced this problem. They came in and changed the level sensors and we have not had the problem since. Christie UAB Hospital Birmingham, AL> From: dellav@musc.edu> To: histonet@lists.utsouthwestern.edu> Date: Fri, 23 Jan 2009 15:13:36 -0500> Subject: [Histonet] LabVision/Dako Autostainer > > We have earlier generation Dako Autostainers and are evaluating a current generation LabVision stainer. New or old we have experienced occasions when this instrument design has failed to dispense reagent. This seems to occur randomly or if there is a pattern we haven't figured it out. I've recently learned that some owners of the Biocare Nemesis which is essentially the same instrument have also encountered this problem.> > The arm will travel to the correct slide but nothing dispenses from the probe, then the arm moves on its way. > > I'd appreciate learning if others have experienced this problem and if a solution to the problem was discovered I hope you'll share it with me.> > > > Vinnie Della Speranza> Manager for Anatomic Pathology Services> Medical University of South Carolina> 165 Ashley Avenue Suite 309> Charleston, South Carolina 29425> Tel: (843) 792-6353> Fax: (843) 792-8974> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Fri Jan 23 15:24:12 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Jan 23 15:24:17 2009 Subject: [Histonet] RE: Prion Contamination-Post Fixation In-Reply-To: <2b268fbd0003579a@HolyRedeemer.com> Message-ID: Ooops, my bad!..you are both right! Another reason that prion contaminated tissues should be left to the experts. And for the rest of us, just read the links for yourself, and try to be safe! Thanks, Terri Terri L. Braud, HT(ASCP) My recollection I believe that it stated that the formic acid was a post-fixation step. Joelle Weaver HTL(ASCP) All, The formic acid is a post-fixation step. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From drvet_anjan <@t> hotmail.com Fri Jan 23 23:06:58 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Fri Jan 23 23:07:02 2009 Subject: [Histonet] methyl green - multiple labelling Message-ID: hi everybody, has anyone tried methyl green as counter stain in multiple label immunohistochemistry. i tried one but i got a dull back ground when i took a snap through Image pro plus 5.1. regards, anjan. _________________________________________________________________ For the freshest Indian Jobs Visit MSN Jobs http://www.in.msn.com/jobs From tifei <@t> foxmail.com Sat Jan 24 01:03:06 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Sat Jan 24 01:03:25 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gbWV0aHlsIGdyZWVuIC0gbXVsdGlwbGUgbGFiZWxsaW5n?= References: Message-ID: <200901241502597473319@foxmail.com> yes methyl blue & methyle green are too dim.. try neutral red...? it seems fast green/light green might lead to intense staining? I am not sure, you may test it. 2009-01-24 TF ???? anjan kumar ????? 2009-01-24 13:11:19 ???? triple immunohistochem ??? ??? [Histonet] methyl green - multiple labelling hi everybody, has anyone tried methyl green as counter stain in multiple label immunohistochemistry. i tried one but i got a dull back ground when i took a snap through Image pro plus 5.1. regards, anjan. _________________________________________________________________ For the freshest Indian Jobs Visit MSN Jobs http://www.in.msn.com/jobs_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Sat Jan 24 06:11:04 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sat Jan 24 06:11:13 2009 Subject: [Histonet] Sectioning on different microtomes Message-ID: <512199.871.qm@web65716.mail.ac4.yahoo.com> How are people lining up the microtomes so they cut in approximately the plane.? I use the old fashioned "test paraffin block" method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own.? Our paths have complained about recuts being too deep.? HELP!!! Kristen From rjbuesa <@t> yahoo.com Sat Jan 24 10:04:08 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 24 10:04:13 2009 Subject: [Histonet] Sectioning on different microtomes In-Reply-To: <512199.871.qm@web65716.mail.ac4.yahoo.com> Message-ID: <983252.56068.qm@web65702.mail.ac4.yahoo.com> Meet with your techs and ask about which angle they prefer and why. Get to a consensus about a "compromise angle" they all can agree with and IMPLEMENT the angle. Put it in the SOP and check that everybody complies. Ren? J. --- On Sat, 1/24/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] Sectioning on different microtomes To: "histonet" Date: Saturday, January 24, 2009, 7:11 AM How are people lining up the microtomes so they cut in approximately the plane.? I use the old fashioned "test paraffin block" method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own.? Our paths have complained about recuts being too deep.? HELP!!! Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sat Jan 24 12:46:06 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sat Jan 24 12:46:12 2009 Subject: [Histonet] Re: CPT coding during accession Message-ID: Tere Hodges at St. Mary's Hospital in Tucson, Arizona asks: >>When you put in charges as a preliminary charge ... do you over bill or under bill? I have always stayed under while putting in charges and then audit the doctor's charges the next day. Example: colon segment other then tumor... bill 88307 the next day tumor was found now it is bumped up to 88309<< In my experience in a number of institutions, doing the CPT coding when you accession the specimen is a bad idea - most of the coding errors never get caught. CPT coding should be done at sign-out or afterward. Remember that a number of common skin lesions are 88304 rather than 88305 depending on the microscopic diagnosis, and that coding a hysterectomy specimen is a dark art that I don't like to make anybody else take responsibility for (except maybe Harry Potter, who after all can cast a patronus charm). I think the responsible pathologist should do the coding (this is a common practice, but not common enough), and that somebody else (like you) should check the pathologist's coding to make sure that nothing has been omitted (decalcification, the routine special stain that was useless in a particular case). Bob Richmond Samurai Pathologist Knoxville TN From jqb7 <@t> cdc.gov Sat Jan 24 18:00:22 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Sat Jan 24 18:14:53 2009 Subject: [Histonet] Sectioning on different microtomes References: <512199.871.qm@web65716.mail.ac4.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE111A721@LTA3VS011.ees.hhs.gov> I simply align my block holder to match up with the block. We get many outside blocks so I have learned to be pretty good at this. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Sat 1/24/2009 7:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned "test paraffin block" method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From G.Spoelstra <@t> murdoch.edu.au Mon Jan 26 03:58:34 2009 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Mon Jan 26 03:58:43 2009 Subject: [Histonet] staining for manganese Message-ID: Hi everybody, I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. Gerard Spoelstra veterinary histology Murdoch University Western Australia From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jan 26 06:04:54 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jan 26 06:05:01 2009 Subject: [Histonet] Microtome alignment Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635CCF@hpes1.HealthPartners.int> We use the microtome alignment instrument, which can be purchased from several companies. It is in our weekly checklist that all microtomes have to be adjusted (checked) with the microtome aligner. All microtomes are cut at the same angle also! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From wilson_c <@t> ricerca.com Mon Jan 26 07:39:34 2009 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Mon Jan 26 07:39:50 2009 Subject: [Histonet] LFB on GMA sections Message-ID: <9D443EB9D0270143B5AAF190CB1A58A309746D3F@dogwood.ricerca.com> Hi all, Can anyone tell me if LFB stain works well for staining nerves in GMA sections? My pathologist is looking at sections of these stained elsewhere and she does not feel the myelin is staining as it should. Any insight on this particular combination? Thanks in advance. Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC From b-frederick <@t> northwestern.edu Mon Jan 26 07:40:51 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jan 26 07:41:04 2009 Subject: [Histonet] Sectioning on different microtomes In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE111A721@LTA3VS011.ees.hhs.gov> Message-ID: I'm on the same page as you Jenaine- we get blocks from all over so we are constantly rearranging the block holder. We also do have a histocollimeter to get the Microms back in line (it doesn't fit on the Leicas. Berncie Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Saturday, January 24, 2009 6:00 PM To: arvidsonkristen@yahoo.com; histonet Subject: RE: [Histonet] Sectioning on different microtomes I simply align my block holder to match up with the block. We get many outside blocks so I have learned to be pretty good at this. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Sat 1/24/2009 7:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned "test paraffin block" method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Mon Jan 26 08:08:22 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jan 26 08:08:31 2009 Subject: [Histonet] Re: CPT coding during accession In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3A5B@IS-E2K3.grhs.net> Well said Bob! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, January 24, 2009 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: CPT coding during accession Tere Hodges at St. Mary's Hospital in Tucson, Arizona asks: >>When you put in charges as a preliminary charge ... do you over bill >>or under bill? I have always stayed under while putting in charges and then audit the doctor's charges the next day. Example: colon segment other then tumor... bill 88307 the next day tumor was found now it is bumped up to 88309<< In my experience in a number of institutions, doing the CPT coding when you accession the specimen is a bad idea - most of the coding errors never get caught. CPT coding should be done at sign-out or afterward. Remember that a number of common skin lesions are 88304 rather than 88305 depending on the microscopic diagnosis, and that coding a hysterectomy specimen is a dark art that I don't like to make anybody else take responsibility for (except maybe Harry Potter, who after all can cast a patronus charm). I think the responsible pathologist should do the coding (this is a common practice, but not common enough), and that somebody else (like you) should check the pathologist's coding to make sure that nothing has been omitted (decalcification, the routine special stain that was useless in a particular case). Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SharonC <@t> celligent.net Mon Jan 26 08:20:53 2009 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Mon Jan 26 08:20:58 2009 Subject: [Histonet] ISH probes Message-ID: Hello Histonetters! We are finding ourselves coming short on the time remaining that we can offer ISH Probes through Ventana. We have not heard when or if ISH (In-situ hybridization) will be offered any more through them. Does anyone out there know of other companies that would offer ISH? Thank you for your input. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net From sbreeden <@t> nmda.nmsu.edu Mon Jan 26 09:04:45 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Jan 26 09:04:50 2009 Subject: [Histonet] New Mexico Society for Histology Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6644@nmdamailsvr.nmda.ad.nmsu.edu> The Board of Directors of the NMSH met on Saturday to discuss the future of the Society. Because membership has declined to less than 10% of eligible members despite concerted efforts over the past five years to increase interest and participation, the decision was made to inactivate the Society. This decision was not reached without serious consideration and although the inactivation of the Society was not the decision we had hoped to make, the declining membership and participation made this move necessary. We will encourage our members to continue membership in NSH and in adjoining State Societies. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From susanbachus <@t> verizon.net Mon Jan 26 09:36:37 2009 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Mon Jan 26 09:36:50 2009 Subject: [Histonet] ISH probes References: Message-ID: <4AC6C103B7CB44669B2323FDCE350B4C@RESLAPTOP> There's a wonderful company called Oligo's, Etc. (Wilsonville, OR) that synthesizes very affordable oligoprobes. But you have to supply the sequence information. Susan ----- Original Message ----- From: "Sharon Campbell" To: Sent: Monday, January 26, 2009 9:20 AM Subject: [Histonet] ISH probes Hello Histonetters! We are finding ourselves coming short on the time remaining that we can offer ISH Probes through Ventana. We have not heard when or if ISH (In-situ hybridization) will be offered any more through them. Does anyone out there know of other companies that would offer ISH? Thank you for your input. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 26 09:57:24 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 26 09:57:32 2009 Subject: [Histonet] staining for manganese In-Reply-To: Message-ID: <561573.10902.qm@web65703.mail.ac4.yahoo.com> Gerard: Many minerals can be "seen" in tissues as an unspecific "black"?particles when reacting with reagents like ammonium sulfide in the Timm's procedure for copper in?Wilson's disease cases. This method requires treating the sections with 0.1N HCl after the reaction to eliminate iron and zinc sulfides that may have been formed. In the same way asbestos can either be detected by ultra filtration of the macerated lung tissue or using the Pearl's iron reaction that will stain the iron contents of the proteinic ?"asbestos bodies" formed around the non-reactive asbestos fibers. I do not know of any specific reaction for manganese in tissues, although I think that the Timm's method without the acid treatment may reveal?an "unspecific" reaction. If that occurs my advise will be to chemically determine the presence of manganese in positively reacting gills?against non reactive gills from other animals as controls. In any event my advise is to avoid the use of benzidine, or to use it with extreme caution because it is a very well known carcinogen, as DAB is also. Sorry I cannot be?more helpful. Ren? J.? --- On Mon, 1/26/09, Gerard Spoelstra wrote: From: Gerard Spoelstra Subject: [Histonet] staining for manganese To: histonet@lists.utsouthwestern.edu Date: Monday, January 26, 2009, 4:58 AM Hi everybody, I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. Gerard Spoelstra veterinary histology Murdoch University Western Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrobertson <@t> pathologysciences.com Mon Jan 26 12:20:24 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Mon Jan 26 12:20:32 2009 Subject: [Histonet] Sectioning on different microtomes In-Reply-To: <512199.871.qm@web65716.mail.ac4.yahoo.com> References: <512199.871.qm@web65716.mail.ac4.yahoo.com> Message-ID: <518CD6920AA7154193CBE5977CD88073177E4E@psmgsrv2.PSMG.local> There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than the paraffin block method. Hope this helps. Jodie Robertson, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, January 24, 2009 4:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane.? I use the old fashioned "test paraffin block" method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own.? Our paths have complained about recuts being too deep.? HELP!!! Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Mon Jan 26 12:25:09 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Jan 26 12:25:12 2009 Subject: [Histonet] Sectioning on different microtomes In-Reply-To: <518CD6920AA7154193CBE5977CD88073177E4E@psmgsrv2.PSMG.local> References: <512199.871.qm@web65716.mail.ac4.yahoo.com> <518CD6920AA7154193CBE5977CD88073177E4E@psmgsrv2.PSMG.local> Message-ID: <497E0005.8010003@pathology.washington.edu> I don't know how these aligning tools work, but I always used an inexpensive level from the hardware store. You need one that can read in the vertical position. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jodie Robertson wrote: > There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than the paraffin block method. Hope this helps. > > Jodie Robertson, HT (ASCP) QIHC > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson > Sent: Saturday, January 24, 2009 4:11 AM > To: histonet > Subject: [Histonet] Sectioning on different microtomes > > How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned "test paraffin block" method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! > Kristen > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From benoit.delatour <@t> u-psud.fr Mon Jan 26 14:23:43 2009 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Beno=EEt_Delatour?=) Date: Mon Jan 26 14:23:32 2009 Subject: [Histonet] Re: staining for manganese In-Reply-To: <20090126180802.4FA7959EDD0@smtp2.u-psud.fr> References: <20090126180802.4FA7959EDD0@smtp2.u-psud.fr> Message-ID: <497E1BCF.5040008@u-psud.fr> Hi, There is a Timm stain - like protocol from Angenstein (Manganese-enhanced MRI reveals structural and functional changes in the cortex of Bassoon mutant mice. Angenstein F, Niessen HG, Goldschmidt J, Lison H, Altrock WD, Gundelfinger ED, Scheich H. Cereb Cortex. 2007 Jan;17(1):28-36) using Na2S that shows some interesting results with manganese autometallography. The problem, as mentionned by RJ Buesa, concerns the specificity of the staining. Using this method in the past I observed staining increase in the brain following MnCl injection. Regards, Beno?t Delatour > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 26 Jan 2009 18:58:34 +0900 > From: "Gerard Spoelstra" > Subject: [Histonet] staining for manganese > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > Hi everybody, > > I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. > > Gerard Spoelstra > veterinary histology > Murdoch University > Western Australia > From jnocito <@t> satx.rr.com Mon Jan 26 19:38:39 2009 From: jnocito <@t> satx.rr.com (JoeNocito) Date: Mon Jan 26 19:38:25 2009 Subject: [Histonet] ASCP Recert Message-ID: <000901c9801f$fb3780f0$f1a682d0$@rr.com> I know sometimes I am not the brightest bulb in the package, the sharpest knife in the drawer, the sharpest tool in the shed, etc. This is the first time that I'm renewing my certifications with ASCP and do not quit understand the process. I have my PA, HT and QIHC. According to the instructions, I have to fill out the Declaration Form for all three certs. Is it me, or does this seem redundant? Because PAs need 45 CMEs, there isn't enough room on the form to list all my courses, so I was going to attach the remaining credits. Do I have to make two copies for my HT and QIHC also? Can I just submit one completed (all 4 pages) form for my PA and attach the first page for my other two certs? Would it make sense for ASCP to have a person list all their certifications and fill out one form? Am I being unreasonable? Why should one have to fill out multiple, four page forms? Please help!!! Thanks JTT From sjromey <@t> bellsouth.net Tue Jan 27 07:20:47 2009 From: sjromey <@t> bellsouth.net (sjromey@bellsouth.net) Date: Tue Jan 27 07:20:55 2009 Subject: [Histonet] microtome angle Message-ID: <012720091320.27205.497F0A2F000D886600006A4522230650629B0A02D2089B9A019C04040A0DBF970A03019D069C@att.net> Good morning, I am having a problem with my microtome angle. I have a reichert-jung 2030 microtome. I cut mainly prostate biopsies and some cervical-ecc biopsies and a few skins. What is a good setting for the angle? Thanks in advance From MLafrini <@t> csmlab.com Tue Jan 27 07:41:12 2009 From: MLafrini <@t> csmlab.com (Michael LaFriniere) Date: Tue Jan 27 07:41:39 2009 Subject: [Histonet] Work flow, quality issues In-Reply-To: <399174.47147.qm@web65716.mail.ac4.yahoo.com> References: <174479.25186.qm@web38201.mail.mud.yahoo.com> <399174.47147.qm@web65716.mail.ac4.yahoo.com> Message-ID: <497EC897.588C.00AF.0@csmlab.com> Steven, It would be my suggestion that the Histology team get together to include the supervisor to facilitate and address the quality issues that you, your labs Pathologist (s) and Director are experiencing since there has been questions raised in this arena. The "team" needs to come up with a plan to meet the needs to demonstrate increased quality and/or productivity. If all are on board and "bought in" to a change, the results and plan will happen much more quickly and smoothly. The Supervisor/Manager should be in a position to take this responsibility, motivate staff as well as personal initiative. Steven, It appears from what you explained the Supervisor/Manager need to step up to the plate to understand your concern of all your associates/coworkers and that your methodology may increase quality and/or productivity with demonstrating by example. (what you explained, sounds like a nightmare)! You do know what its like to be in a new vehicle, the technology, the feel, the looks the smell...? Every Histologist has a certain talent and if it should be ignored by the Supervisor/Manager/Director/Pathologist, it may be time to give your seat up on that old bus and get on a much newer model to allow you the opportunity to feel good at what do what you do best...fortunate for us in histology /pathology there are plenty of new vehicles out there ! Your current employer is fortunate to have an employee that demonstrates care and compassion for high quality expectations for the patient! Michael Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 1/15/2009 at 12:29:16 PM, Rene J Buesa wrote: Steven: Are you just complaining to vent some sort of frustration, or can you actually do something about it? If venting, my sympathies go to you. What you describe could become the nightmare of anybody with the slightest idea about histology good practices, not to mention somebody that tries to take the flow into a "more or less" Lean semblance. The "muda" is astonishing. If you can do something about, what you describe you are used to do is just what has to be done. Use your knowledge and credentials to try to solve the mess you describe. You know how to do it and good luck. I am sure that "somebody" in that lab has done it that way for years and will be difficult to made to change. Ren? J. --- On Wed, 1/14/09, Steven Coakley wrote: From: Steven Coakley Subject: [Histonet] Work flow, quality issues To: Histonet@lists.utsouthwestern.edu Date: Wednesday, January 14, 2009, 3:53 PM I have worked in several HT labs and as expected most differ in individual technique. Most are very good as far as work flow from grossing, processing, embedding and sectioning. I work in a lab now where the grossing and processing of "like specimens" are in case order until there embedded. Embedded totally out of order, even within a case. One person will rough cut the blocks on 1 microtome, approx 20 microns. After all the embedding is done the trimmed blocks are put in order, placed on ice in which about half are to be cut on another microtome. Although the microtomes are adjusted close I have found that by the time I'm ready to section my blocks on the microtome not used for trimming I often have to go into the tissue 20-40microns, 5 microns at a time, to get a complete section. This often makes it tough to get a good, rehydrated 2nd section not to mention often the 1st if the event the tissue is larger and or firm to start with. Often I have to re-trim the blocks to match my microtome then rehydrate them again. This all takes time and makes it impossible to section all my cases in order, waiting on blocks to rehydrate, hold slides sometimes leading to mistakes. I have always, and in every lab I worked except this one, trimm ed my own blocks for a specific microtome. At the end of trimming, I always fine cut 4-5 microns 2-5 times to deminish the artifact often caused by too aggressive initial trimming. Then I rehydrate and ice the tissue.. With this technique I use less knifes also. I temped in this lab about 1/1/2 years ago and was asked by the Pathologist and Lab Director to address these very issues and section quality. Now that I'm back nothing has changed. The pathologist still has section quality issues. What ever happen to the idea of Quality Improvement. The works getting done I suppose, maybe thats all that matters these days. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue Jan 27 08:36:57 2009 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jan 27 08:37:13 2009 Subject: [Histonet] ASCP Recert In-Reply-To: <000901c9801f$fb3780f0$f1a682d0$@rr.com> References: <000901c9801f$fb3780f0$f1a682d0$@rr.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE636@EXCHANGEBE1.carle.com> Joe, I just recertified. Call me 217-383-3666 Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JoeNocito Sent: Monday, January 26, 2009 7:39 PM To: histonet Subject: [Histonet] ASCP Recert I know sometimes I am not the brightest bulb in the package, the sharpest knife in the drawer, the sharpest tool in the shed, etc. This is the first time that I'm renewing my certifications with ASCP and do not quit understand the process. I have my PA, HT and QIHC. According to the instructions, I have to fill out the Declaration Form for all three certs. Is it me, or does this seem redundant? Because PAs need 45 CMEs, there isn't enough room on the form to list all my courses, so I was going to attach the remaining credits. Do I have to make two copies for my HT and QIHC also? Can I just submit one completed (all 4 pages) form for my PA and attach the first page for my other two certs? Would it make sense for ASCP to have a person list all their certifications and fill out one form? Am I being unreasonable? Why should one have to fill out multiple, four page forms? Please help!!! Thanks JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jan 27 09:33:52 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jan 27 09:34:09 2009 Subject: [Histonet] microtome angle In-Reply-To: <012720091320.27205.497F0A2F000D886600006A4522230650629B0A02D2089B9A019C04040A0DBF970A03019D069C@att.net> Message-ID: HI In regards to your question about knife angle. I have a few questions. Are you using a steele knife or disposible blade.? If the blade is disposible are you using high or low profile blades? Please get back with me when you can and we can discuss off line. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com sjromey@bellsouth .net Sent by: To histonet-bounces@ Histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] microtome angle 01/27/2009 07:20 AM Good morning, I am having a problem with my microtome angle. I have a reichert-jung 2030 microtome. I cut mainly prostate biopsies and some cervical-ecc biopsies and a few skins. What is a good setting for the angle? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From rjbuesa <@t> yahoo.com Tue Jan 27 09:50:16 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 27 09:50:25 2009 Subject: [Histonet] microtome angle In-Reply-To: <012720091320.27205.497F0A2F000D886600006A4522230650629B0A02D2089B9A019C04040A0DBF970A03019D069C@att.net> Message-ID: <962200.54001.qm@web65702.mail.ac4.yahoo.com> The sectioning angle is one of the trickiest aspects of the "art of microtomy" and is not something that can be written on stone. The angle that you need to obtain a good section (either individual or serial) will depend on any or all of the following factors: 1- the type of blade (steel permanent or disposable of low or high profile); 2- the type of paraffin used; 3- the block temperature; 4- the type of tissue and how well (or not) it was infiltrated, meaning that the tissue processing will have also an effect; 5- the size and shape?of the block/tissue; and 6- the sectioning speed, amongst other minor "adjustments". ? On the other hand it will be extremely difficult to change the angle for each block or circumstance, so the best course of action is to have a standard tissue processing, a standard type of blade or knife, select an angle of?about 15? and work the block by changing the temperature and sectioning speed, i.e., let the sectioning speed "take care" of the differences between blocks. It has always worked for me. Ren? J. --- On Tue, 1/27/09, sjromey@bellsouth.net wrote: From: sjromey@bellsouth.net Subject: [Histonet] microtome angle To: Histonet@lists.utsouthwestern.edu Date: Tuesday, January 27, 2009, 8:20 AM Good morning, I am having a problem with my microtome angle. I have a reichert-jung 2030 microtome. I cut mainly prostate biopsies and some cervical-ecc biopsies and a few skins. What is a good setting for the angle? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Jan 27 09:50:38 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 27 09:50:45 2009 Subject: [Histonet] RELIA Histology Careers Job Alert 1-27-09 Message-ID: Hi Histonetters! Wow 2009 is kicking into gear. New positions are coming in from all over the U. S. on an almost daily basis! I have some great new opportunities and thought I would post them so you could take a look. All of these jobs are full time permanent dayshift positions unless otherwise noted. My clients offer great compensation and benefits. NEW POSITIONS: Histology Supervisor ? Atlanta, GA Histology Supervisor Night Shift ? Ft. Lauderdale, FL Histology Manager ? Long Island, NY Histotechnician/Histotechnologist ASCP or eligible San Francisco, CA Histotechnician/Histotechnologist 2nd Shift North Shore of Boston, MA Histotechnician/Histotechnologist 2nd and 3rd shift positions Ft. Lauderdale, FL Histotechnician/Histotechnologist Temple, TX I also have management positions in Boston, MA and Los Angeles, CA and Histo Tech positions in WA, CA, PA, TX, MA and OH If you or anyone you know might be interested in any of these opportunities I can be reached toll free at 866-607-3542 or relia1@earthlink.net Remember, if you know someone who might be interested I also offer a$500 referral bonus and if I place you a $500 hiring bonus. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thanks ? Pam 866-60-RELIA (866-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia < http://www.myspace.com/pamatrelia> From ian.montgomery <@t> bio.gla.ac.uk Tue Jan 27 12:14:14 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Jan 27 12:14:24 2009 Subject: [Histonet] Wax. Message-ID: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> Paraffin wax is giving a few problems with some tissues. What is the current favourite from UK suppliers? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From sbreeden <@t> nmda.nmsu.edu Tue Jan 27 13:26:49 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jan 27 13:26:57 2009 Subject: [Histonet] Green counterstain Issues Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> My (working) light green counterstain (Sheehan, Theory & Practice..., pg 187) does its thing unevenly. Given, a lot of our tissue is poorly fixed to begin with or possibly autolytic, but I get more staining around the periphery and little or no staining in the body of the section. What am I missing here? Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Tue Jan 27 14:46:17 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 27 14:46:24 2009 Subject: [Histonet] Green counterstain Issues In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <132392.68543.qm@web65705.mail.ac4.yahoo.com> That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than adequate time in the fixative. Increase the fixing time and most likely your staining unevenness will disappear. Ren? J. --- On Tue, 1/27/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Green counterstain Issues To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 27, 2009, 2:26 PM My (working) light green counterstain (Sheehan, Theory & Practice..., pg 187) does its thing unevenly. Given, a lot of our tissue is poorly fixed to begin with or possibly autolytic, but I get more staining around the periphery and little or no staining in the body of the section. What am I missing here? Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Tue Jan 27 15:31:58 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Jan 27 15:32:12 2009 Subject: [Histonet] pathology assistants Message-ID: Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From Charles.Embrey <@t> carle.com Tue Jan 27 16:44:45 2009 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jan 27 16:44:54 2009 Subject: [Histonet] pathology assistants In-Reply-To: References: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> Are the looking for Pathology Assistants or Pathologists' Assistants? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Tuesday, January 27, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology assistants Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LVOGT <@t> lewistownhospital.org Tue Jan 27 18:54:27 2009 From: LVOGT <@t> lewistownhospital.org (VOGT, LORI) Date: Tue Jan 27 18:54:38 2009 Subject: [Histonet] second patient identifier on tissue cassette Message-ID: How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? ***************************************************************************************** Lori A. Vogt Technical Section Head/ Histology Lewistown Hospital 400 Highland Ave. Lewistown, PA 17044 Phone-717-242-7213 Fax-717-242-7540 Web Site: http://www.lewistownhospital.org The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. ***************************************************************************************** The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. From pieronelva01 <@t> bigpond.com Tue Jan 27 21:02:06 2009 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Tue Jan 27 21:02:13 2009 Subject: [Histonet] second patient identifier on tissue cassette References: Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F@pentium4> Before we had our cassette labeller, we wrote the first three letters only of the surname on the side. Pretty awkward, but we had no other option. Piero Nelva Anatomical PAthology Monash Medical Centre Australia ----- Original Message ----- From: "VOGT, LORI" To: Sent: Wednesday, January 28, 2009 11:54 AM Subject: [Histonet] second patient identifier on tissue cassette How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? ***************************************************************************************** Lori A. Vogt Technical Section Head/ Histology Lewistown Hospital 400 Highland Ave. Lewistown, PA 17044 Phone-717-242-7213 Fax-717-242-7540 Web Site: http://www.lewistownhospital.org The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. ***************************************************************************************** The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.176 / Virus Database: 270.10.14/1920 - Release Date: 1/27/2009 6:15 PM From j.h.reilly <@t> clinmed.gla.ac.uk Wed Jan 28 03:49:06 2009 From: j.h.reilly <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Wed Jan 28 03:49:16 2009 Subject: [Histonet] Wax. In-Reply-To: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> References: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> Message-ID: Hello Ian In the GBRC we are using Histoplast PE from Thermo Scientific. Jim James H Reilly Sir Graeme Davies Building GBRC 120 university Place Glasgow G12 8TA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: 27 January 2009 18:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wax. Paraffin wax is giving a few problems with some tissues. What is the current favourite from UK suppliers? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Wed Jan 28 07:06:22 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Jan 28 07:06:37 2009 Subject: [Histonet] pathology assistants In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> Message-ID: 2 assistants. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. "Charles.Embrey" 01/27/2009 02:44 PM To Marilyn A Weiss/CA/KAIPERM@KAIPERM, cc Subject RE: [Histonet] pathology assistants Are the looking for Pathology Assistants or Pathologists' Assistants? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Tuesday, January 27, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology assistants Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Wed Jan 28 08:20:31 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 28 08:20:37 2009 Subject: [Histonet] second patient identifier on tissue cassette In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> Where in the CAP Standard check list for Anatomic Pathology does it state that a second patient identifier must be on the tissue cassette? I don't remember reading that. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, LORI Sent: Tuesday, January 27, 2009 6:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] second patient identifier on tissue cassette How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? ************************************************************************ ***************** Lori A. Vogt Technical Section Head/ Histology Lewistown Hospital 400 Highland Ave. Lewistown, PA 17044 Phone-717-242-7213 Fax-717-242-7540 Web Site: http://www.lewistownhospital.org The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. ************************************************************************ ***************** The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LVOGT <@t> lewistownhospital.org Wed Jan 28 08:45:14 2009 From: LVOGT <@t> lewistownhospital.org (VOGT, LORI) Date: Wed Jan 28 08:45:19 2009 Subject: [Histonet] second patient identifier on tissue cassette In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> Message-ID: I know it is not a Cap requirement yet. JACHO is requiring 2 patient identifier on specimens and I am looking to the future trickle down effect. Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps. -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, January 28, 2009 9:21 AM To: VOGT, LORI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] second patient identifier on tissue cassette Where in the CAP Standard check list for Anatomic Pathology does it state that a second patient identifier must be on the tissue cassette? I don't remember reading that. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, LORI Sent: Tuesday, January 27, 2009 6:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] second patient identifier on tissue cassette How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? ************************************************************************ ***************** Lori A. Vogt Technical Section Head/ Histology Lewistown Hospital 400 Highland Ave. Lewistown, PA 17044 Phone-717-242-7213 Fax-717-242-7540 Web Site: http://www.lewistownhospital.org The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. ************************************************************************ ***************** The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Wed Jan 28 09:27:31 2009 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Jan 28 09:28:11 2009 Subject: [Histonet] pathology assistants Message-ID: <498033130200001A00028F75@GWIA2.umm.edu> LOL! You didnt answer the question and theres a Big Difference >>> 01/28/09 8:09 AM >>> 2 assistants. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. "Charles.Embrey" 01/27/2009 02:44 PM To Marilyn A Weiss/CA/KAIPERM@KAIPERM, cc Subject RE: [Histonet] pathology assistants Are the looking for Pathology Assistants or Pathologists' Assistants? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Tuesday, January 27, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology assistants Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Brenda <@t> nsh.org Wed Jan 28 10:43:53 2009 From: Brenda <@t> nsh.org (Brenda Royce) Date: Wed Jan 28 10:43:58 2009 Subject: [Histonet] NSH 2009 Winter Lunch and Learn Message-ID: Registration is now open for the first NSH Winter Lunch & Learn, presented in partnership with Leica Microsystems, taking place February 28, 2009 at Jacksonville Community College in Jacksonville, FL. Attendees have an opportunity to attend 3 sessions and earn 4.5 contact hours in this half day program. Schedule: 8:00am - 9:00am: Registration 9:00am - 10:30am: Small Specimen Management - In a Large Volume World 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of Change in Histotechnology All sessions are presented by top rated NSH presenters Skip Brown, Leica BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems. Its a great inexpensive way to expand your knowledge & earn those needed contact hours. Visit our website to: www.nsh.org Register Online or Download a PDF of the Registration Form We hope to see you there! From ian.montgomery <@t> bio.gla.ac.uk Wed Jan 28 12:38:00 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Jan 28 12:38:09 2009 Subject: [Histonet] 2045 Message-ID: <83FCECB271BA45618AF156B1A4086D21@IBLS.GLA.AC.UK> Just came into possession of a Leica 2045 Supercut that been sitting in a lab collecting dust. I used the instrument many years ago but I just cannot remember how to take the control pad from lock to fully active. I can remember all the other function buttons, but the combination to take it off lock has been deleted from my memory banks. In case you think I'm daft, I have switched it on and the lock has a red light. I've sworn at it, promised it a good thrashing, sulked and cried but nothing, the red lock light is still on. So, anyone using a 2045 and what is the secret? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From tbraud <@t> holyredeemer.com Wed Jan 28 13:13:26 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jan 28 13:13:34 2009 Subject: [Histonet] RE: 2 patient identifiers In-Reply-To: <44e1752900051e2f@HolyRedeemer.com> Message-ID: Our Hospital is 272 beds. We recently purchased the Leica IPS for labeling slides, and the Leica IPC for labeling cassettes. We use both printers using Leica's software, independent of our LIS. The techs LOVE them and the Pathologists and techs love the cassette printer the best. We put the accession number on one line, and the patient last name, first initial, on the second line. They are extremely easy to read and having the name helps with block filing. Administration was all too willing to foot the bill to support the 2 patient identifiers on such a critical piece of the patient workflow. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax Message: 6 Date: Tue, 27 Jan 2009 19:54:27 -0500 From: "VOGT, LORI" Subject: [Histonet] second patient identifier on tissue cassette To: How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? ***************************************************************************************** Lori A. Vogt Technical Section Head/ Histology Lewistown Hospital --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From jessgrocki <@t> yahoo.com Wed Jan 28 13:18:58 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Wed Jan 28 13:19:01 2009 Subject: [Histonet] Procedure for Immunoperoxidase on a Frozen Section Message-ID: <669254.66955.qm@web82005.mail.mud.yahoo.com> ? Hi All, Does anyone have a procedure (using a Dako Autostainer or other Immunostainer) for Immunoperoxidase on Frozen Sections and touch preps, that you wouldn't mind sharing with me? Thanks! ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From NHeath <@t> Lifespan.org Wed Jan 28 13:42:13 2009 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Wed Jan 28 13:42:33 2009 Subject: [Histonet] standards for sectioning & embedding Message-ID: <130E8991F210424096EFC6F42EA33B2403EAE8B4@LSCOEXCH1.lsmaster.lifespan.org> Hi, What standards do you Histo folks out there require of new student hires and seasoned techs for the amount of slides sectioned and blocks embedded. Thanks, Nacy From litepath2000 <@t> yahoo.com Wed Jan 28 13:51:36 2009 From: litepath2000 <@t> yahoo.com (NYSHistotech) Date: Wed Jan 28 13:51:40 2009 Subject: [Histonet] NYSHS 2009 Annual Symposium Announcement Message-ID: <702085.16969.qm@web58805.mail.re1.yahoo.com> Dear?Histology Professional The New York State Histotechnological Society is please to announce its 2009 annual symposium to be held at the Holiday Inn in Fishkill, New York on April 24th and 25th, 2009. Our academic program offers over 25 contact hours of CEU's on a wide range of topics. For a copy of the program, registration forms or additional information,?please visit the New York State Histotechnological Society website?at: www.nyhisto.org? We hope to see you there. NYSHS Staff --------? NYSHS Website www.nyhisto.org NYSHS Message Board http://tech.groups.yahoo.com/group/NYSHS1972/ From melissa.mazan <@t> tufts.edu Wed Jan 28 14:11:19 2009 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Wed Jan 28 14:11:34 2009 Subject: [Histonet] nuclear counterstain In-Reply-To: <200901281803.n0SI3MBb020665@mail-proofpoint-8a.usg.tufts.edu> References: <200901281803.n0SI3MBb020665@mail-proofpoint-8a.usg.tufts.edu> Message-ID: <4980BBE7.2050904@tufts.edu> Hi all, I'm staining mouse lung tissues with Dako's Ki67 using Vector ABC Elite and DAB to visualize - I get lovely sharp black brown nuclei where appropriate. There is almost no background staining. However, no matter how lightly I counterstain, I lose the definition between DAB and counterstain - I have tried hematoxylin and Nuclear Red - any suggestions? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Wax. (Ian Montgomery) > 2. Green counterstain Issues (Breeden, Sara) > 3. Re: Green counterstain Issues (Rene J Buesa) > 4. pathology assistants (Marilyn.A.Weiss@kp.org) > 5. RE: pathology assistants (Charles.Embrey) > 6. second patient identifier on tissue cassette (VOGT, LORI) > 7. Re: second patient identifier on tissue cassette (Piero Nelva) > 8. RE: Wax. (Jim Reilly) > 9. RE: pathology assistants (Marilyn.A.Weiss@kp.org) > 10. RE: second patient identifier on tissue cassette (Mike Pence) > 11. RE: second patient identifier on tissue cassette (VOGT, LORI) > 12. RE: pathology assistants (Kimberly Tuttle) > 13. NSH 2009 Winter Lunch and Learn (Brenda Royce) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Jan 2009 18:14:14 -0000 > From: "Ian Montgomery" > Subject: [Histonet] Wax. > To: > Message-ID: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Paraffin wax is giving a few problems with some tissues. What is > the current favourite from UK suppliers? > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Jan 2009 12:26:49 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Green counterstain Issues > To: > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Jan 2009 12:46:17 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu, "Breeden, Sara" > > Message-ID: <132392.68543.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than adequate time in the fixative. Increase the fixing time and most likely your staining unevenness will disappear. > Ren? J. > > --- On Tue, 1/27/09, Breeden, Sara wrote: > > From: Breeden, Sara > Subject: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, January 27, 2009, 2:26 PM > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Jan 2009 13:31:58 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] pathology assistants > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > > > ------------------------------ > > Message: 5 > Date: Tue, 27 Jan 2009 16:44:45 -0600 > From: "Charles.Embrey" > Subject: RE: [Histonet] pathology assistants > To: , > Message-ID: > <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> > Content-Type: text/plain; charset="us-ascii" > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 27 Jan 2009 19:54:27 -0500 > From: "VOGT, LORI" > Subject: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > > > ------------------------------ > > Message: 7 > Date: Wed, 28 Jan 2009 14:02:06 +1100 > From: "Piero Nelva" > Subject: Re: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F@pentium4> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Before we had our cassette labeller, we wrote the first three letters only > of the surname on the side. Pretty awkward, but we had no other option. > > Piero Nelva > Anatomical PAthology > Monash Medical Centre > Australia > > > ----- Original Message ----- > From: "VOGT, LORI" > To: > Sent: Wednesday, January 28, 2009 11:54 AM > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on the > tissue cassette if you do not have a cassette printer/labeler? Is there an > easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of our > communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed above. > If you are neither the intended recipient or the employee or agent > responsible for delivering this information to the intended recipient, you > are hereby notified that any disclosure, copying, distribution, or taking of > any action in reliance on the content of this telecopied information is > strictly prohibited. If you receive this e-mail in error, please immediately > notify us by telephone to arrange for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------------- > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.176 / Virus Database: 270.10.14/1920 - Release Date: 1/27/2009 > 6:15 PM > > > > > ------------------------------ > > Message: 8 > Date: Wed, 28 Jan 2009 09:49:06 -0000 > From: "Jim Reilly" > Subject: RE: [Histonet] Wax. > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello Ian > > In the GBRC we are using Histoplast PE from Thermo Scientific. > > Jim > > James H Reilly > Sir Graeme Davies Building > GBRC > 120 university Place > Glasgow > G12 8TA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian > Montgomery > Sent: 27 January 2009 18:14 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax. > > > Paraffin wax is giving a few problems with some tissues. > What is the current favourite from UK suppliers? > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 28 Jan 2009 05:06:22 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: RE: [Histonet] pathology assistants > To: Charles.Embrey@carle.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > 2 assistants. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > > > > > "Charles.Embrey" > 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Wed, 28 Jan 2009 08:20:31 -0600 > From: "Mike Pence" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "VOGT, LORI" , > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 28 Jan 2009 09:45:14 -0500 > From: "VOGT, LORI" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "Mike Pence" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I know it is not a Cap requirement yet. JACHO is requiring 2 patient identifier on specimens and I am looking to the future trickle down effect. Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps. > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Wednesday, January 28, 2009 9:21 AM > To: VOGT, LORI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] second patient identifier on tissue cassette > > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 28 Jan 2009 10:27:31 -0500 > From: "Kimberly Tuttle" > Subject: RE: [Histonet] pathology assistants > To: , > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <498033130200001A00028F75@GWIA2.umm.edu> > Content-Type: text/plain; charset=US-ASCII > > LOL! You didnt answer the question and theres a Big Difference > > > >>>> 01/28/09 8:09 AM >>> >>>> > 2 assistants. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > > > > > "Charles.Embrey" > 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > > > ------------------------------ > > Message: 13 > Date: Wed, 28 Jan 2009 11:43:53 -0500 > From: "Brenda Royce" > Subject: [Histonet] NSH 2009 Winter Lunch and Learn > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Registration is now open for the first NSH Winter Lunch & Learn, > presented in partnership with Leica Microsystems, taking place February > 28, 2009 at Jacksonville Community College in Jacksonville, FL. > Attendees have an opportunity to attend 3 sessions and earn 4.5 contact > hours in this half day program. > > Schedule: > 8:00am - 9:00am: Registration > 9:00am - 10:30am: Small Specimen Management - In a Large Volume World > 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer > 12:00pm - 12:30pm: Lunch Provided by NSH > 12:30pm - 2:00pm: The Paradox of Change in Histotechnology > > All sessions are presented by top rated NSH presenters Skip Brown, Leica > BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems. > > Its a great inexpensive way to expand your knowledge & earn those needed > contact hours. > > Visit our website to: www.nsh.org > > Register Online > vt_key=6c37f294-9716-4f7c-a65e-4b7b89435387&RegPath=EventRegFees> or > Download a PDF of the Registration Form > > > We hope to see you there! > > > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 62, Issue 34 > **************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu From lpaveli1 <@t> hurleymc.com Wed Jan 28 14:23:08 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jan 28 14:23:48 2009 Subject: [Histonet] nuclear counterstain Message-ID: <4980785D020000EE00025E85@smtp-gw.hurleymc.com> Melissa, We also have struggled with Nuclear Fast Red in the past. We tried "Brazilliant!" from Anatech, and it stains much nicer; a darker/clearer red. Hope this helps. Lynette >>> Melissa Mazan 01/28/09 3:11 PM >>> Hi all, I'm staining mouse lung tissues with Dako's Ki67 using Vector ABC Elite and DAB to visualize - I get lovely sharp black brown nuclei where appropriate. There is almost no background staining. However, no matter how lightly I counterstain, I lose the definition between DAB and counterstain - I have tried hematoxylin and Nuclear Red - any suggestions? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Wax. (Ian Montgomery) > 2. Green counterstain Issues (Breeden, Sara) > 3. Re: Green counterstain Issues (Rene J Buesa) > 4. pathology assistants (Marilyn.A.Weiss@kp.org) > 5. RE: pathology assistants (Charles.Embrey) > 6. second patient identifier on tissue cassette (VOGT, LORI) > 7. Re: second patient identifier on tissue cassette (Piero Nelva) > 8. RE: Wax. (Jim Reilly) > 9. RE: pathology assistants (Marilyn.A.Weiss@kp.org) > 10. RE: second patient identifier on tissue cassette (Mike Pence) > 11. RE: second patient identifier on tissue cassette (VOGT, LORI) > 12. RE: pathology assistants (Kimberly Tuttle) > 13. NSH 2009 Winter Lunch and Learn (Brenda Royce) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Jan 2009 18:14:14 -0000 > From: "Ian Montgomery" > Subject: [Histonet] Wax. > To: > Message-ID: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Paraffin wax is giving a few problems with some tissues. What is > the current favourite from UK suppliers? > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Jan 2009 12:26:49 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Green counterstain Issues > To: > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Jan 2009 12:46:17 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu, "Breeden, Sara" > > Message-ID: <132392.68543.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than ademe and most likely your staining unevenness will disappear. > Ren? J. > > --- On Tue, 1/27/09, Breeden, Sara wrote: > > From: Breeden, Sara > Subject: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, January 27, 2009, 2:26 PM > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Jan 2009 13:31:58 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] pathology assistants > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > > > ------------------------------ > > Message: 5 > Date: Tue, 27 Jan 2009 16:44:45 -0600 > From: "Charles.Embrey" > Subject: RE: [Histonet] pathology assistants > To: , > Message-ID: > <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> > Content-Type: text/plain; charset="us-ascii" > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 27 Jan 2009 19:54:27 -0500 > From: "VOGT, LORI" > Subject: [Histonet] second patient identifier on tissue cassette > To: > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > > > ------------------------------ > > Message: 7 > Date: Wed, 28 Jan 2009 14:02:06 +1100 > From: "Piero Nelva" > Subject: Re: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F@pentium4> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Before we had our cassette labeller, we wrote the first three letters only > of the surname on the side. Pretty awkward, but we had no other option. > > Piero Nelva > Anatomical PAthology > Monash Medical Centre > Australia > > > ----- Original Message ----- > From: "VOGT, LORI" > To: > Sent: Wednesday, January 28, 2009 11:54 AM > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on the > tissue cassette if you do not have a cassette printer/labeler? Is there an > easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of our > communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed above. > If you are neither the intended recipient or the employee or agent > responsible for delivering this information to the intended recipient, you > are hereby notified that any disclosure, copying, distribution, or taking of > any action in reliance on the content of this telecopied information is > strictly prohibited. If you receive this e-mail in error, please immediately > notify us by telephone to arrange for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------------- > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > > > > > ------------------------------ > > Message: 8 > Date: Wed, 28 Jan 2009 09:49:06 -0000 > From: "Jim Reilly" > Subject: RE: [Histonet] Wax. > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello Ian > > In the GBRC we are using Histoplast PE from Thermo Scientific. > > Jim > > James H Reilly > Sir Graeme Davies Building > GBRC > 120 university Place > Glasgow > G12 8TA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian > Montgomery > Sent: 27 January 2009 18:14 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax. > > > Paraffin wax is giving a few problems with some tissues. > What is the current favourite from UK suppliers? > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 28 Jan 2009 05:06:22 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: RE: [Histonet] pathology assistants > To: Charles.Embrey@carle.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > 2 assistants. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > > > > > "Charles.Embrey" > 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Wed, 28 Jan 2009 08:20:31 -0600 > From: "Mike Pence" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "VOGT, LORI" , > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> > Content-Type: text/plaines it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 28 Jan 2009 09:45:14 -0500 > From: "VOGT, LORI" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "Mike Pence" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I know it is not a Cap requirement yet. JACHO is requiring 2 patient identifier on specimens and I am looking to the future trickle down effect. Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps. > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Wednesday, January 28, 2009 9:21 AM > To: VOGT, LORI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] second patient identifier on tissue cassette > > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to pro************************************************************** > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 28 Jan 2009 10:27:31 -0500 > From: "Kimberly Tuttle" > Subject: RE: [Histonet] pathology assistants > To: , > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <498033130200001A00028F75@GWIA2.umm.edu> > Content-Type: text/plain; charset=US-ASCII > > LOL! You didnt answer the question and theres a Big Difference > > > >>>> 01/28/09 8:09 AM >>> >>>> > 2 assistants. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > > > > > "Charles.Embrey" > 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great > benefits.If you have any questions or would like to send your resume, > please contact Diane I Johnson at Kaiser Permanente. at > Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephrg> > Subject: [Histonet] NSH 2009 Winter Lunch and Learn > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Registration is now open for the first NSH Winter Lunch & Learn, > presented in partnership with Leica Microsystems, taking place February > 28, 2009 at Jacksonville Community College in Jacksonville, FL. > Attendees have an opportunity to attend 3 sessions and earn 4.5 contact > hours in this half day program. > > Schedule: > 8:00am - 9:00am: Registration > 9:00am - 10:30am: Small Specimen Management - In a Large Volume World > 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer > 12:00pm - 12:30pm: Lunch Provided by NSH > 12:30pm - 2:00pm: The Paradox of Change in Histotechnology > > All sessions are presented by top rated NSH presenters Skip Brown, Leica > BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems. > > Its a great inexpensive way to expand your knowledge & earn those needed > contact hours. > > Visit our website to: www.nsh.org > > Register Online > vt_key=6c37f294-9716-4f7c-a65e-4b7b89435387&RegPath=EventRegFees> or > Download a PDF of the Registration Form > > > We hope to see you there! > > > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 62, Issue 34 > **************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john_ronan <@t> merck.com Wed Jan 28 14:49:20 2009 From: john_ronan <@t> merck.com (Ronan, John) Date: Wed Jan 28 14:49:30 2009 Subject: [Histonet] RE:second patient identifier In-Reply-To: References: Message-ID: <2B73727E2E719B46BCB34456948AF54F800A85@USCTMX1140.merck.com> Lori, Working in a research lab, I do not know all the requirements concerning a second patient identifier, but I can relate to a small lab without a cassette printer. Would it be possible to use sequentially pre-labeled cassettes that you can purchase? The cassettes I use have two fields, the human read cassette number & the barcode version of the number. In my case, I have set up a database that will spit out study number, animal number, group description ect. from the cassette number. In your case, the pathologist would read the cassette number into his/her dictation and now the patient number, name, and all pertinent info including a description of the content of the cassette can be linked to the cassette number. It actually eliminates the need to write on the cassette at all. John Ronan Research Associate Laboratory Animal Resources 732 594-6378 -----Original Message----- Date: Tue, 27 Jan 2009 19:54:27 -0500 From: "VOGT, LORI" Subject: [Histonet] second patient identifier on tissue cassette To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? ************************************************************************ ***************** Lori A. Vogt Technical Section Head/ Histology Lewistown Hospital 400 Highland Ave. Lewistown, PA 17044 Phone-717-242-7213 Fax-717-242-7540 Web Site: http://www.lewistownhospital.org Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Marilyn.A.Weiss <@t> kp.org Wed Jan 28 14:52:41 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Jan 28 14:52:58 2009 Subject: [Histonet] pathology assistants In-Reply-To: <498033130200001A00028F75@GWIA2.umm.edu> Message-ID: we need 2 qualified patholgists assistants to cut in large specimens, assist with autopsies, and maintain the gross room. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. "Kimberly Tuttle" 01/28/2009 07:27 AM To , Marilyn A Weiss/CA/KAIPERM@KAIPERM cc Subject RE: [Histonet] pathology assistants LOL! You didnt answer the question and theres a Big Difference >>> 01/28/09 8:09 AM >>> 2 assistants. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. "Charles.Embrey" 01/27/2009 02:44 PM To Marilyn A Weiss/CA/KAIPERM@KAIPERM, cc Subject RE: [Histonet] pathology assistants Are the looking for Pathology Assistants or Pathologists' Assistants? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Tuesday, January 27, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology assistants Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From rjbuesa <@t> yahoo.com Wed Jan 28 15:47:41 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 28 15:47:43 2009 Subject: [Histonet] Procedure for Immunoperoxidase on a Frozen Section In-Reply-To: <669254.66955.qm@web82005.mail.mud.yahoo.com> Message-ID: <130140.5491.qm@web65715.mail.ac4.yahoo.com> For either one you can follow the same protocol as for FFPE sections without the HIER (is not necessary). Ren? J. --- On Wed, 1/28/09, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Procedure for Immunoperoxidase on a Frozen Section To: "histonet" Date: Wednesday, January 28, 2009, 2:18 PM ? Hi All, Does anyone have a procedure (using a Dako Autostainer or other Immunostainer) for Immunoperoxidase on Frozen Sections and touch preps, that you wouldn't mind sharing with me? Thanks! ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 28 15:49:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 28 15:49:43 2009 Subject: [Histonet] standards for sectioning & embedding In-Reply-To: <130E8991F210424096EFC6F42EA33B2403EAE8B4@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <956691.20913.qm@web65703.mail.ac4.yahoo.com> For "seasoned" histotechs to embed 60 blocks/hour and section 24 blocks/hour. For students you could start at half that amount and check their progress with time. Ren? J. --- On Wed, 1/28/09, Heath, Nancy L. wrote: From: Heath, Nancy L. Subject: [Histonet] standards for sectioning & embedding To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 28, 2009, 2:42 PM Hi, What standards do you Histo folks out there require of new student hires and seasoned techs for the amount of slides sectioned and blocks embedded. Thanks, Nacy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 28 15:52:05 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 28 15:52:15 2009 Subject: [Histonet] nuclear counterstain In-Reply-To: <4980BBE7.2050904@tufts.edu> Message-ID: <203997.83269.qm@web65706.mail.ac4.yahoo.com> If you obtain the detail you need with the DAB, eliminate the counter stain altogether because really you do not need it. Ren? J. --- On Wed, 1/28/09, Melissa Mazan wrote: From: Melissa Mazan Subject: [Histonet] nuclear counterstain To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 28, 2009, 3:11 PM Hi all, I'm staining mouse lung tissues with Dako's Ki67 using Vector ABC Elite and DAB to visualize - I get lovely sharp black brown nuclei where appropriate. There is almost no background staining. However, no matter how lightly I counterstain, I lose the definition between DAB and counterstain - I have tried hematoxylin and Nuclear Red - any suggestions? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Wax. (Ian Montgomery) > 2. Green counterstain Issues (Breeden, Sara) > 3. Re: Green counterstain Issues (Rene J Buesa) > 4. pathology assistants (Marilyn.A.Weiss@kp.org) > 5. RE: pathology assistants (Charles.Embrey) > 6. second patient identifier on tissue cassette (VOGT, LORI) > 7. Re: second patient identifier on tissue cassette (Piero Nelva) > 8. RE: Wax. (Jim Reilly) > 9. RE: pathology assistants (Marilyn.A.Weiss@kp.org) > 10. RE: second patient identifier on tissue cassette (Mike Pence) > 11. RE: second patient identifier on tissue cassette (VOGT, LORI) > 12. RE: pathology assistants (Kimberly Tuttle) > 13. NSH 2009 Winter Lunch and Learn (Brenda Royce) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Jan 2009 18:14:14 -0000 > From: "Ian Montgomery" > Subject: [Histonet] Wax. > To: > Message-ID: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Paraffin wax is giving a few problems with some tissues. What is > the current favourite from UK suppliers? > > Ian. > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Jan 2009 12:26:49 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Green counterstain Issues > To: > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Jan 2009 12:46:17 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu, "Breeden, Sara" > > Message-ID: <132392.68543.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than adequate time in the fixative. Increase the fixing time and most likely your staining unevenness will disappear. > Ren? J. > > --- On Tue, 1/27/09, Breeden, Sara wrote: > > From: Breeden, Sara > Subject: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, January 27, 2009, 2:26 PM > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Jan 2009 13:31:58 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] pathology assistants > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > ------------------------------ > > Message: 5 > Date: Tue, 27 Jan 2009 16:44:45 -0600 > From: "Charles.Embrey" > Subject: RE: [Histonet] pathology assistants > To: , > Message-ID: > <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> > Content-Type: text/plain; charset="us-ascii" > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 27 Jan 2009 19:54:27 -0500 > From: "VOGT, LORI" > Subject: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > > > ------------------------------ > > Message: 7 > Date: Wed, 28 Jan 2009 14:02:06 +1100 > From: "Piero Nelva" > Subject: Re: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F@pentium4> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Before we had our cassette labeller, we wrote the first three letters only of the surname on the side. Pretty awkward, but we had no other option. > > Piero Nelva > Anatomical PAthology > Monash Medical Centre > Australia > > > ----- Original Message ----- From: "VOGT, LORI" > To: > Sent: Wednesday, January 28, 2009 11:54 AM > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------------- > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.176 / Virus Database: 270.10.14/1920 - Release Date: 1/27/2009 6:15 PM > > > > > ------------------------------ > > Message: 8 > Date: Wed, 28 Jan 2009 09:49:06 -0000 > From: "Jim Reilly" > Subject: RE: [Histonet] Wax. > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello Ian > > In the GBRC we are using Histoplast PE from Thermo Scientific. > > Jim > > James H Reilly Sir Graeme Davies Building > GBRC > 120 university Place > Glasgow > G12 8TA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian > Montgomery > Sent: 27 January 2009 18:14 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax. > > > Paraffin wax is giving a few problems with some tissues. > What is the current favourite from UK suppliers? > > Ian. > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 28 Jan 2009 05:06:22 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: RE: [Histonet] pathology assistants > To: Charles.Embrey@carle.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > 2 assistants. > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > > > "Charles.Embrey" 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Wed, 28 Jan 2009 08:20:31 -0600 > From: "Mike Pence" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "VOGT, LORI" , > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 28 Jan 2009 09:45:14 -0500 > From: "VOGT, LORI" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "Mike Pence" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I know it is not a Cap requirement yet. JACHO is requiring 2 patient identifier on specimens and I am looking to the future trickle down effect. Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps. > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Wednesday, January 28, 2009 9:21 AM > To: VOGT, LORI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] second patient identifier on tissue cassette > > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 28 Jan 2009 10:27:31 -0500 > From: "Kimberly Tuttle" > Subject: RE: [Histonet] pathology assistants > To: , > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <498033130200001A00028F75@GWIA2.umm.edu> > Content-Type: text/plain; charset=US-ASCII > > LOL! You didnt answer the question and theres a Big Difference > > > >>>> 01/28/09 8:09 AM >>> >>>> > 2 assistants. > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > > > "Charles.Embrey" 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > > > ------------------------------ > > Message: 13 > Date: Wed, 28 Jan 2009 11:43:53 -0500 > From: "Brenda Royce" > Subject: [Histonet] NSH 2009 Winter Lunch and Learn > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Registration is now open for the first NSH Winter Lunch & Learn, > presented in partnership with Leica Microsystems, taking place February > 28, 2009 at Jacksonville Community College in Jacksonville, FL. > Attendees have an opportunity to attend 3 sessions and earn 4.5 contact > hours in this half day program. > Schedule: > 8:00am - 9:00am: Registration 9:00am - 10:30am: Small Specimen Management - In a Large Volume World > 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer > 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of Change in Histotechnology > All sessions are presented by top rated NSH presenters Skip Brown, Leica > BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems. > Its a great inexpensive way to expand your knowledge & earn those needed > contact hours. > Visit our website to: www.nsh.org > > Register Online > vt_key=6c37f294-9716-4f7c-a65e-4b7b89435387&RegPath=EventRegFees> or > Download a PDF of the Registration Form > > We hope to see you there! > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 62, Issue 34 > **************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atellez <@t> crf.org Wed Jan 28 16:30:47 2009 From: atellez <@t> crf.org (Armando Tellez) Date: Wed Jan 28 16:30:54 2009 Subject: [Histonet] Plastic embedding Message-ID: Hi, Does anyone have a protocol for plastic embedding that shows in detail the procedure and equipment needed for this purpose that does not mind share it? My office email is attached to my signature. Please let me know since my boss want to start a stent pathology protocls and he claims that we have the equipment, so first I need a good full blown descriptive protocol and all the protocols in internet are already assuming that you have some plastic embedding knowledge and I have never embed any metal devices. (I need a protocol for microtome, not grinding) Thanks Armando Tellez atellez@crf.org Pathology Department Tel: (845) 290 8034 Fax: (845) 290 8030 From macveigh <@t> usc.edu Wed Jan 28 19:38:13 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Jan 28 19:38:49 2009 Subject: [Histonet] Need any info for JUNG HISTOSTAINER Ig Message-ID: <001301c981b2$40370840$5c237d80@DFS66DD1> Hi all, We do research. We got a hand me down JUNG AUTOSTAINER XL which now does all the H&E and it is a blessing! Now I am thinking about purchasing a second hand Immunostainer to run some immunos, so we can use our time for something better than babysitting the slides. There is a very inexpensive JUNG HISTOSTAINER Ig available, but I have no experience in this area. (I couldn't even find a picture of it on the Internet...) For now we only do a TUNEL stain for apoptosis, but the need for more immunos is rising. (Our students use a lot of fluorescent markers if this makes any difference.) What do you think? Do you recommend it or am I just investing in a pricey incubation chamber? I would appreciate any input Michelle From digakuinsei <@t> yahoo.com Thu Jan 29 05:27:17 2009 From: digakuinsei <@t> yahoo.com (Yobou IG) Date: Thu Jan 29 05:27:28 2009 Subject: [Histonet] MAD2 Immunocytochemistry Message-ID: <989360.3605.qm@web58505.mail.re3.yahoo.com> Dear All I am trying to detect kinetochore localization of MAD2 induced by a microtubule depolymerizing agent in SW480 human cancer cells.?I have used 2 different protocols?(-20 oC 100% methanol fixation for 20 minutes?or 4% formaldehyde fixation for 20 minutes, both followed by permeabilization with either 0.3% triton/PBS or 100 micog/mL digitonin/PBS)?but unfortunately I got negative results. I had success with microtubules immunocytochemical staining using methanol fixation method and I demonstrated the induction of prometaphase arrest by my treament, but it seems that MAD2 immunostaining is not as easy as microtubules. I would greatly appreciate if anyone has an experience in MAD2 immunocytochemical staining provides me with a help. thanks a lot Regards, Yobou I.G., M.Sc. PhD graduate student kyoto prefectural university of medicine Japan From Marilyn.A.Weiss <@t> kp.org Thu Jan 29 06:34:49 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Jan 29 06:35:01 2009 Subject: [Histonet] Re: Histonet Digest, Vol 62, Issue 35 In-Reply-To: <200901282153.n0SLrjY1016618@mail.kp.org> Message-ID: Please take off my previous posting of looking for Pathology Assistant's and post that Kaiser is looking for 2 Pathologist's Assistant's. Send resume to Diane.I.Johnson@kp.org or call 619-528-5386. I would appreciate that. Was in a hurry and wanted to help out my boss, so sorry for the confusion. I certainly know the difference. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From CDunn <@t> mtsinai.on.ca Thu Jan 29 06:56:16 2009 From: CDunn <@t> mtsinai.on.ca (Dunn, Cyndy) Date: Thu Jan 29 06:56:21 2009 Subject: [Histonet] Recycled solvents and chatter Message-ID: <2009D9CA27C1B8418B551D309223B74902617CFD@mshmail16> Does anyone experience chatter they think originates from using recycled solvents in their tissue processors? We have tried many things to eliminate the chatter. The latest was removing the three recycled xylenes from the processor and replacing them with fresh Commercial xylene. After doing this the Pathologist was very pleased with the sections, no chatter in eight blocks, three levels each. From Beth.Fye <@t> HCAhealthcare.com Thu Jan 29 07:47:20 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Thu Jan 29 07:47:39 2009 Subject: [Histonet] 2nd patient identifier on cassettes Message-ID: <938F8EC5A524D34EB5796E23E52781D3039F603529@NADCWPMSGCMS05.hca.corpad.net> We average over 400 cassettes a day, and put the Accession # on the top of the cassette, the last name on one side and tissue type on the other side. We are currently still doing all this by hand, but are looking into getting slide and cassette labelers. We started doing this as a matter of safety and not by any regulatory standard 5-6 years ago. It is a procedure that has saved us many times over the years. It is very rare to have cassettes that are mislabeled by both the accession # and patients name. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 From BMolinari <@t> heart.thi.tmc.edu Thu Jan 29 07:49:48 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Jan 29 07:49:54 2009 Subject: [Histonet] Plastic embedding In-Reply-To: Message-ID: You and the rest of the world Armando. Information like this is costly and hard to come by. Best of luck none the less. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77225 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Armando Tellez Sent: Wednesday, January 28, 2009 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic embedding Hi, Does anyone have a protocol for plastic embedding that shows in detail the procedure and equipment needed for this purpose that does not mind share it? My office email is attached to my signature. Please let me know since my boss want to start a stent pathology protocls and he claims that we have the equipment, so first I need a good full blown descriptive protocol and all the protocols in internet are already assuming that you have some plastic embedding knowledge and I have never embed any metal devices. (I need a protocol for microtome, not grinding) Thanks Armando Tellez atellez@crf.org Pathology Department Tel: (845) 290 8034 Fax: (845) 290 8030 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Thu Jan 29 08:02:20 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Thu Jan 29 08:02:49 2009 Subject: [Histonet] nuclear counterstain In-Reply-To: <203997.83269.qm@web65706.mail.ac4.yahoo.com> References: <4980BBE7.2050904@tufts.edu> <203997.83269.qm@web65706.mail.ac4.yahoo.com> Message-ID: <5B6165D78AC14544974A844787B47E380703CCEF@MAIL5.ad.uams.edu> Hi Melissa: When definition is compromised I use a 1% light green counterstain for 30 seconds. My boss thinks it is great. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 28, 2009 3:52 PM To: histonet@lists.utsouthwestern.edu; Melissa Mazan Subject: Re: [Histonet] nuclear counterstain If you obtain the detail you need with the DAB, eliminate the counter stain altogether because really you do not need it. Ren? J. --- On Wed, 1/28/09, Melissa Mazan wrote: From: Melissa Mazan Subject: [Histonet] nuclear counterstain To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 28, 2009, 3:11 PM Hi all, I'm staining mouse lung tissues with Dako's Ki67 using Vector ABC Elite and DAB to visualize - I get lovely sharp black brown nuclei where appropriate. There is almost no background staining. However, no matter how lightly I counterstain, I lose the definition between DAB and counterstain - I have tried hematoxylin and Nuclear Red - any suggestions? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Wax. (Ian Montgomery) > 2. Green counterstain Issues (Breeden, Sara) > 3. Re: Green counterstain Issues (Rene J Buesa) > 4. pathology assistants (Marilyn.A.Weiss@kp.org) > 5. RE: pathology assistants (Charles.Embrey) > 6. second patient identifier on tissue cassette (VOGT, LORI) > 7. Re: second patient identifier on tissue cassette (Piero Nelva) > 8. RE: Wax. (Jim Reilly) > 9. RE: pathology assistants (Marilyn.A.Weiss@kp.org) > 10. RE: second patient identifier on tissue cassette (Mike Pence) > 11. RE: second patient identifier on tissue cassette (VOGT, LORI) > 12. RE: pathology assistants (Kimberly Tuttle) > 13. NSH 2009 Winter Lunch and Learn (Brenda Royce) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Jan 2009 18:14:14 -0000 > From: "Ian Montgomery" > Subject: [Histonet] Wax. > To: > Message-ID: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Paraffin wax is giving a few problems with some tissues. What is > the current favourite from UK suppliers? > > Ian. > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Jan 2009 12:26:49 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Green counterstain Issues > To: > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Jan 2009 12:46:17 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu, "Breeden, Sara" > > Message-ID: <132392.68543.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than adequate time in the fixative. Increase the fixing time and most likely your staining unevenness will disappear. > Ren? J. > > --- On Tue, 1/27/09, Breeden, Sara wrote: > > From: Breeden, Sara > Subject: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, January 27, 2009, 2:26 PM > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Jan 2009 13:31:58 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] pathology assistants > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > ------------------------------ > > Message: 5 > Date: Tue, 27 Jan 2009 16:44:45 -0600 > From: "Charles.Embrey" > Subject: RE: [Histonet] pathology assistants > To: , > Message-ID: > <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> > Content-Type: text/plain; charset="us-ascii" > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 27 Jan 2009 19:54:27 -0500 > From: "VOGT, LORI" > Subject: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > > > ------------------------------ > > Message: 7 > Date: Wed, 28 Jan 2009 14:02:06 +1100 > From: "Piero Nelva" > Subject: Re: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F@pentium4> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Before we had our cassette labeller, we wrote the first three letters only of the surname on the side. Pretty awkward, but we had no other option. > > Piero Nelva > Anatomical PAthology > Monash Medical Centre > Australia > > > ----- Original Message ----- From: "VOGT, LORI" > To: > Sent: Wednesday, January 28, 2009 11:54 AM > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------------- > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.176 / Virus Database: 270.10.14/1920 - Release Date: 1/27/2009 6:15 PM > > > > > ------------------------------ > > Message: 8 > Date: Wed, 28 Jan 2009 09:49:06 -0000 > From: "Jim Reilly" > Subject: RE: [Histonet] Wax. > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello Ian > > In the GBRC we are using Histoplast PE from Thermo Scientific. > > Jim > > James H Reilly Sir Graeme Davies Building > GBRC > 120 university Place > Glasgow > G12 8TA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian > Montgomery > Sent: 27 January 2009 18:14 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax. > > > Paraffin wax is giving a few problems with some tissues. > What is the current favourite from UK suppliers? > > Ian. > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 28 Jan 2009 05:06:22 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: RE: [Histonet] pathology assistants > To: Charles.Embrey@carle.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > 2 assistants. > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > > > "Charles.Embrey" 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Wed, 28 Jan 2009 08:20:31 -0600 > From: "Mike Pence" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "VOGT, LORI" , > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 28 Jan 2009 09:45:14 -0500 > From: "VOGT, LORI" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "Mike Pence" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I know it is not a Cap requirement yet. JACHO is requiring 2 patient identifier on specimens and I am looking to the future trickle down effect. Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps. > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Wednesday, January 28, 2009 9:21 AM > To: VOGT, LORI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] second patient identifier on tissue cassette > > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 28 Jan 2009 10:27:31 -0500 > From: "Kimberly Tuttle" > Subject: RE: [Histonet] pathology assistants > To: , > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <498033130200001A00028F75@GWIA2.umm.edu> > Content-Type: text/plain; charset=US-ASCII > > LOL! You didnt answer the question and theres a Big Difference > > > >>>> 01/28/09 8:09 AM >>> >>>> > 2 assistants. > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > > > "Charles.Embrey" 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > > > ------------------------------ > > Message: 13 > Date: Wed, 28 Jan 2009 11:43:53 -0500 > From: "Brenda Royce" > Subject: [Histonet] NSH 2009 Winter Lunch and Learn > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Registration is now open for the first NSH Winter Lunch & Learn, > presented in partnership with Leica Microsystems, taking place February > 28, 2009 at Jacksonville Community College in Jacksonville, FL. > Attendees have an opportunity to attend 3 sessions and earn 4.5 contact > hours in this half day program. > Schedule: > 8:00am - 9:00am: Registration 9:00am - 10:30am: Small Specimen Management - In a Large Volume World > 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer > 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of Change in Histotechnology > All sessions are presented by top rated NSH presenters Skip Brown, Leica > BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems. > Its a great inexpensive way to expand your knowledge & earn those needed > contact hours. > Visit our website to: www.nsh.org > > Register Online > vt_key=6c37f294-9716-4f7c-a65e-4b7b89435387&RegPath=EventRegFees> or > Download a PDF of the Registration Form > > We hope to see you there! > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 62, Issue 34 > **************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 29 08:07:35 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 29 08:07:40 2009 Subject: [Histonet] Patient identifier Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635D04@hpes1.HealthPartners.int> According to the "rule" regarding 2 patient identifiers, it is our understanding that the 2 identifiers must be on the container label and in the computer system upon accessioning. When the computer assigns the surgical case number, all info is linked to that listed surgical accessioned number and your 2 or more identifiers are pulled up with the case. That also keeps within "HIPPA" guidelines to have patient information openly available when blocks or slides are sent out or left out in view. Otherwise, barcoding is the ideal way to place, view, and tie all information needed!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From foreightl <@t> gmail.com Thu Jan 29 08:21:07 2009 From: foreightl <@t> gmail.com (Pat Laurie) Date: Thu Jan 29 08:21:14 2009 Subject: [Histonet] Klotskin's modification of the Masson's Trichrome Message-ID: Hi Everyone, Has anyone heard of the Klotskin's modification of the Masson's Trichrome? I am working with a liver pathologist who wants me to try to work it up. I can't seem to find it anywhere. I have tried numerous books, and scoured the internet. Someone asked about 3 years ago on histonet, but there were no replies. If anyone could help me I would appreciate it. Thanks, -- Patrick Laurie (HT, ASCP) Cellnetix Pathology and Laboratories Seattle, Wa From koellingr <@t> comcast.net Thu Jan 29 08:31:53 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Jan 29 08:31:56 2009 Subject: [Histonet] Klotskin's modification of the Masson's Trichrome Message-ID: <012920091431.24615.4981BDD90001504B0000602722070229339D09020704040A0105@comcast.net> Isn't a Klatskin tumor (of the biliary tree) spelled with A and not O? There are multiple pictures and references to Klatskin's Trichrome. Try A spelling and not O. Ray Koelling Research Pathology PhenoPath Labs Seattle -------------- Original message ---------------------- From: Pat Laurie > Hi Everyone, > > Has anyone heard of the Klotskin's modification of the Masson's Trichrome? > I am working with a liver pathologist who wants me to try to work it up. I > can't seem to find it anywhere. I have tried numerous books, and scoured > the internet. Someone asked about 3 years ago on histonet, but there were > no replies. If anyone could help me I would appreciate it. > > Thanks, > > -- > Patrick Laurie (HT, ASCP) > Cellnetix Pathology and Laboratories > Seattle, Wa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Thu Jan 29 08:32:30 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Jan 29 08:32:36 2009 Subject: FW: [Histonet] Klotskin's modification of the Masson's Trichrome Message-ID: Try searching Klatskin Trichrome. Lots of references on internet. Klatskin was a famous liver pathologist. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Thursday, January 29, 2009 8:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Klotskin's modification of the Masson's Trichrome Hi Everyone, Has anyone heard of the Klotskin's modification of the Masson's Trichrome? I am working with a liver pathologist who wants me to try to work it up. I can't seem to find it anywhere. I have tried numerous books, and scoured the internet. Someone asked about 3 years ago on histonet, but there were no replies. If anyone could help me I would appreciate it. Thanks, -- Patrick Laurie (HT, ASCP) Cellnetix Pathology and Laboratories Seattle, Wa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Thu Jan 29 09:10:25 2009 From: foreightl <@t> gmail.com (Pat Laurie) Date: Thu Jan 29 09:10:30 2009 Subject: FW: [Histonet] Klotskin's modification of the Masson's Trichrome In-Reply-To: References: Message-ID: I apologize for the typo, I meant Klatskin, It is very early in the morning here...There are many references, and yes, a couple of photos of the stain, but no actual procedure... On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD wrote: > Try searching Klatskin Trichrome. Lots of references on internet. > Klatskin was a famous liver pathologist. > > > > Lester J. Raff, MD > > Medical Director > > UroPartners Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel 708.486.0076 > > Fax 708.486.0080 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat > Laurie > Sent: Thursday, January 29, 2009 8:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Klotskin's modification of the Masson's Trichrome > > > > Hi Everyone, > > > > Has anyone heard of the Klotskin's modification of the Masson's > Trichrome? > > I am working with a liver pathologist who wants me to try to work it up. > I > > can't seem to find it anywhere. I have tried numerous books, and > scoured > > the internet. Someone asked about 3 years ago on histonet, but there > were > > no replies. If anyone could help me I would appreciate it. > > > > Thanks, > > > > -- > > Patrick Laurie (HT, ASCP) > > Cellnetix Pathology and Laboratories > > Seattle, Wa > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie (HT, ASCP) Virginia Mason Medical Center Seattle, Wa From llewllew <@t> shaw.ca Thu Jan 29 10:07:43 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Jan 29 10:08:13 2009 Subject: FW: [Histonet] Klotskin's modification of the Masson's Trichrome References: Message-ID: Klatskin's Modification of Masson Trichrome Stain 1. Hydrate paraffin sections 2. Harris's hematoxylin, 10 minutes 3. 95% ethanol, rinse parent hepatic fat fraction provides an index that can be 4. Picric alcohol (saturated solution of picric acid in 95% ethanol), 10 minutes 5. Wash, 10 minutes 6. Ponceau (1% ponceau in 1% glacial acetic acid), 5 minutes 7. Phosphomolybdic acid (1%, aqueous), 5 minutes 8. Aniline blue (saturated solution of aniline blue in 2.5% glacial acetic acid), 2 minutes 9. Phosphomolybdic acid, 5 minutes 10. 1% acetic acid, 5 minutes 11. 70% alcohol, 2 minutes 12. Dehydrate and mount http://www3.interscience.wiley.com/cgi-bin/fulltext/106594374/PDFSTART Bryan Llewewllyn ----- Original Message ----- From: "Pat Laurie" To: Sent: Thursday, January 29, 2009 7:10 AM Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's Trichrome >I apologize for the typo, I meant Klatskin, It is very early in the morning > here...There are many references, and yes, a couple of photos of the > stain, > but no actual procedure... > > On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD > wrote: > >> Try searching Klatskin Trichrome. Lots of references on internet. >> Klatskin was a famous liver pathologist. >> >> >> >> Lester J. Raff, MD >> >> Medical Director >> >> UroPartners Laboratory >> >> 2225 Enterprise Dr. Suite 2511 >> >> Westchester, Il 60154 >> >> Tel 708.486.0076 >> >> Fax 708.486.0080 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat >> Laurie >> Sent: Thursday, January 29, 2009 8:21 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Klotskin's modification of the Masson's Trichrome >> >> >> >> Hi Everyone, >> >> >> >> Has anyone heard of the Klotskin's modification of the Masson's >> Trichrome? >> >> I am working with a liver pathologist who wants me to try to work it up. >> I >> >> can't seem to find it anywhere. I have tried numerous books, and >> scoured >> >> the internet. Someone asked about 3 years ago on histonet, but there >> were >> >> no replies. If anyone could help me I would appreciate it. >> >> >> >> Thanks, >> >> >> >> -- >> >> Patrick Laurie (HT, ASCP) >> >> Cellnetix Pathology and Laboratories >> >> Seattle, Wa >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Patrick Laurie (HT, ASCP) > Virginia Mason Medical Center > Seattle, Wa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Thu Jan 29 10:15:41 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Thu Jan 29 10:16:11 2009 Subject: [Histonet] PIN4 Control Message-ID: <8CB5043F733D762-1004-801@FWM-M26.sysops.aol.com> Can someone let me know where I can purchase a good PIN4 control....also, what tissue do you use for the negative when you use one control for multiple cases. Thanks, Ann From rjbuesa <@t> yahoo.com Thu Jan 29 11:03:57 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 29 11:04:01 2009 Subject: [Histonet] PIN4 Control In-Reply-To: <8CB5043F733D762-1004-801@FWM-M26.sysops.aol.com> Message-ID: <894423.77079.qm@web65705.mail.ac4.yahoo.com> Each case ought to have its own negative control. If several cases are treated by the same?antibody you can have one single positive control for that Ab, but the same does not apply to negative controls. ren? J. --- On Thu, 1/29/09, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] PIN4 Control To: histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 11:15 AM Can someone let me know where I can purchase a good PIN4 control....also, what tissue do you use for the negative when you use one control for multiple cases. Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brod033 <@t> gmail.com Thu Jan 29 11:20:00 2009 From: brod033 <@t> gmail.com (Ben Spirto) Date: Thu Jan 29 11:20:04 2009 Subject: [Histonet] quiq decalcifier Message-ID: <287d127c0901290920o6160fda1y7e277aac7adb4a6f@mail.gmail.com> Could someone tell me the best way for quick decalcification of parafin embeded brain tissue. I know about treatment with HCl. Anything else? With something, perhaps that can be found in the biochemical lab??? thanks Ben From POWELL_SA <@t> Mercer.edu Thu Jan 29 11:18:24 2009 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Jan 29 11:26:01 2009 Subject: [Histonet] Georgia Society for Histotechnology Program Message-ID: <21B2510769024081812D5D3A12987F77@powellsa1> The Georgia Society for Histotechnology invites you to the 2009 meeting to be held at Sea Palms Resort at St. Simons Island, Georgia, March 20-22, 2009. The program is below and the registration form can be downloaded from www.histosearch.com/gsh soon or I will email you a copy personally. The complete hotel information is already on the GSH website. Please call for reservations now by calling the Sea Palms Resort. Be sure to tell them you are attending the GSH meeting. The phone number is 1-800-841-6268. Visit the web site at www.seapalms.com. Special GSH Room Rates are $99 for two double beds and $109 for two double beds or a King bed Suites are available as well as Villas. March 20, 2009 - Friday 1 to 5 p.m.: HT/HTL Review Session for Students 5 to 7 p.m.: Meeting Registration 7 to 9 p.m.: Vendor Reception in Vendor Area March 21, 2009 - Saturday 7:00-8:00 a.m.: Meeting Registration 8:30 a.m. to 12: Workshop #1 - Today's Artifacts - Tomorrow's Facts 8:30 a.m. to 12: Workshop #2 - Expense Analysis and Reduction in the IHC Lab (10:00 - 10:30 a.m.: Break in Vendor Area) 12:00 - 1:00-GSH AWARDS LUNCHEON 1:00 to 4:30 p.m.: Workshop #3 - Decalcified and Undecalcified Bone: Histology Techniques 1:00 to 4:30 p.m.: Workshop #4 - Basic Troubleshooting for Histology Laboratory Equipment (2:30 - 3:00 p.m.: Break in Vendor area) 4:30 p.m.: GSH General Membership Meeting (GSH Board Meeting to Follow) March 22, 2009 - Sunday 7:00-8:00 a.m.: Meeting Registration 8:30 to 12 a.m.: Workshop #5 - Commitment in the Workplace - What Does it Mean to the Employee and the Employer 8:30 to 12 a.m.: Workshop #6 - Contemporary Trends in Immunohistochemistry (10:00 - 10:30 a.m.: Break in Vendor Area) WORKSHOP DESCRIPTIONS: #1: Today's Artifacts - Tomorrow's Facts? Lamar Jones, BS, HT(ASCP) - This workshop will teach the participant to recognize and identify artifacts from the gross board, fixation, processing, embedding, microtomy, staining, coverslipping and other areas of histotechnology. #2: Expense Analysis and Reduction in the IHC Lab Joe Myers, - This workshop will discuss ways to cut expenses in the immunohistochemistry laboratory without sacrificing quality. #3: Decalcified and Undecalcified Bone: Histology Techniques Vickie Kalscheur - The speaker will give an overview of Decalcified and Undecalcified bone sample preparation for research histology. Specimen collection, fixation, decalcification, processing, and more will be covered using a wide variety of bone samples. The undecalcified component, again discusses handling, preparation, and staining of undecalcified Plastic embedded bone samples. Special and immunohistochemical staining of bone specimens will be discussed. This talk will be casual and informative for those working in clinical or research settings. Handouts will be provided. #4: Basic Troubleshooting for Histology Laboratory Equipment Jason Velasquez, Technical Engineer -This course will provide a basic preventive maintenance guide that will assist users of histology equipment in the upkeep and troubleshooting of their instruments. The type of cleaning solvents that can and cannot be used will be discussed (along with some pictures that show what happens when the wrong cleaning supplies are used) and how and where to clean for best results. The types of tools that should be kept in the laboratory's tool chest and how and when to use them will be demonstrated. Common types of faults that can be reasonably repaired by the average Histotech will be discussed and the ways, tools and thoughts behind the troubleshooting process will be investigated. Some symptoms that precede failures will be made known so that the users can notify their bio-medical technicians or repair group of a pending failure, before the instrument breaks completely. #5: Commitment in the Workplace - What Does it Mean to the Employee and Employer Wanda Grace Jones, - Hospitals, Research Labs, and Private Laboratories still struggle with continuous loss of employees and finding new employees to fill these positions. Past research has isolated two variables that impact employee turnover. 1st variable is employee's identification with and involvement in an organization (how involved are you). 2nd variable is the employee's perception of level of commitment an organization has to the employee. We will discuss the attitude toward an organization which attaches the person to an organization, the process by which the goals of the organization and employee become integrated, building better communication between employee and employer and cost associated when an employee decides to leave. #6: Contemporary Trends in Immunohistochemistry Mary Cheles, MPH, HTL, DLM(ASCP) - The analysis of a patient has historically relied on morphology and the evaluation of individual antibodies on pathological tissue. Immunohistochemistry has been in practice for the past 40 years. During that time, we have seen an evolution from individual reagents to optimized systems and from manual staining practices to fully automated options. Pathology and laboratory medicine is changing faster than ever. In the future, personalized medicine will define the effect of a therapy based on an individual's gene and protein profile. What does this mean and where does the histology community fit in? This workshop will briefly review immunohistochemistry basics, opportunities for automation, process standardization, antibody validation, regulatory product labeling and current proficiency testing. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 Ph: 478-301-2374 Fx: 478-301-5489 From Barbara.Stancel <@t> fsis.usda.gov Thu Jan 29 12:13:54 2009 From: Barbara.Stancel <@t> fsis.usda.gov (Stancel, Barbara) Date: Thu Jan 29 12:14:03 2009 Subject: [Histonet] free archive of JOH! Message-ID: After 38 years in this profession, it is with great joy that I plan to retire sometime in 2009. I am gradually going though my "archives" (Arranged & Random Collections of Highly Important Volumes Essential to only mySelf). I have worked for the Federal Government for 30 years---everything has an acronym! Among my prized processions is (what I believe to be) a full set of the Journal of Histotechnology. Beginning with Volume 1 No.1 September, 1977 through the one I received yesterday. I really do not have a place for them at home. They will not be conducive to retirement activates. They will contribute nothing to sewing for my beautiful granddaughters and their dolls. Their only benefit in my garden would be as (gasp!) mulch or compost. I am hoping somewhere out there in HistoLand is someone or some institution who would like to maintain these in their archives. Someone who will enjoy the nostalgia of the early editions and the indepth-ness of the most current issues. All I ask is that you pay for the shipping. It will be book rate, but I have no idea how many pounds yet. There are enough to fill at least 1 ? to 2 copier boxes. Or if you are in the great and glorious Southern states of Georgia, Alabama, Tennessee, or South or North Carolinas, you may pay for my husband's and my dinners when we meet to load them from my trunk to yours. Later, I may have some books to sell or donate. It is tough to part with my many histo-bibles. They become an integral part of one's lab life. You know which one to grab for any question. You can loan them out to newbies. But to be sure you get them back : you SIGN them out to Pathologists. They make a great "lift" when your adjustable height chair no longer adjusts and you have blocks to embed. Best of all; what a great way to remember-way-back-when? Hand processing. Our first Autotechnicon with its punched metal disc time controller to the Ultratechnicon with agitation, heat and vacuum! We started processing RUSH specimens in 1.5 to 2 hours. We have come a long way in just 38 short years!!! I digress. Or is it regress? In my case, it's both! Please e-mail me at the address below. Histologically yours, Barbara USDA, FSIS, EL, Pathology Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stancel@fsis.usda.gov From atellez <@t> crf.org Thu Jan 29 12:41:03 2009 From: atellez <@t> crf.org (Armando Tellez) Date: Thu Jan 29 12:41:10 2009 Subject: Subject: RE: [Histonet] Plastic embedding Message-ID: Thank you Mrs. Molinari for your kind response. I would like to thank for all the persons that personally contact me for all the useful information regarding the protocols and equipment for plastic embedding. The full descriptive information that were kindly given by this people will definitely help me in this endeavor. I certainly appreciate this Great network! Armando Tellez, MD From Marilyn.A.Weiss <@t> kp.org Thu Jan 29 12:44:51 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Jan 29 12:45:10 2009 Subject: [Histonet] Patholologist's Assitant's Message-ID: Kaiser is looking for 2 qualified Pathologist's Assistant's. Send resume to Diane.I.Johnson@kp.org or call 619-528-5386. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From lblazek <@t> digestivespecialists.com Thu Jan 29 12:55:32 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 29 12:53:00 2009 Subject: [Histonet] RE: free archive of JOH! In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39086A10F820@IBMB7Exchange.digestivespecialists.com> Barbara, The pack rat in me is screaming "ME ME ME!" But alas if I drag anything more home my husband will be auctioning me off on EBay and if I bring any more into the lab one of my techs will lock me out of the lab. I hope your treasures find a wonderful new home! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stancel, Barbara Sent: Thursday, January 29, 2009 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] free archive of JOH! After 38 years in this profession, it is with great joy that I plan to retire sometime in 2009. I am gradually going though my "archives" (Arranged & Random Collections of Highly Important Volumes Essential to only mySelf). I have worked for the Federal Government for 30 years---everything has an acronym! Among my prized processions is (what I believe to be) a full set of the Journal of Histotechnology. Beginning with Volume 1 No.1 September, 1977 through the one I received yesterday. I really do not have a place for them at home. They will not be conducive to retirement activates. They will contribute nothing to sewing for my beautiful granddaughters and their dolls. Their only benefit in my garden would be as (gasp!) mulch or compost. I am hoping somewhere out there in HistoLand is someone or some institution who would like to maintain these in their archives. Someone who will enjoy the nostalgia of the early editions and the indepth-ness of the most current issues. All I ask is that you pay for the shipping. It will be book rate, but I have no idea how many pounds yet. There are enough to fill at least 1 ? to 2 copier boxes. Or if you are in the great and glorious Southern states of Georgia, Alabama, Tennessee, or South or North Carolinas, you may pay for my husband's and my dinners when we meet to load them from my trunk to yours. Later, I may have some books to sell or donate. It is tough to part with my many histo-bibles. They become an integral part of one's lab life. You know which one to grab for any question. You can loan them out to newbies. But to be sure you get them back : you SIGN them out to Pathologists. They make a great "lift" when your adjustable height chair no longer adjusts and you have blocks to embed. Best of all; what a great way to remember-way-back-when? Hand processing. Our first Autotechnicon with its punched metal disc time controller to the Ultratechnicon with agitation, heat and vacuum! We started processing RUSH specimens in 1.5 to 2 hours. We have come a long way in just 38 short years!!! I digress. Or is it regress? In my case, it's both! Please e-mail me at the address below. Histologically yours, Barbara USDA, FSIS, EL, Pathology Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stancel@fsis.usda.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kevinpe <@t> mail.med.upenn.edu Thu Jan 29 13:03:57 2009 From: kevinpe <@t> mail.med.upenn.edu (Kevin Egan) Date: Thu Jan 29 13:04:05 2009 Subject: [Histonet] re: plastic embedding Message-ID: <490222589.38563981233255837240.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> I am in a similar situation as Armando. My PI recently told me to figure out how to do plastic embedding to view calcein/Xylenol orange staining. I've been following a procedure described in " Immunohistochemistry of matrix markers in Technovit 9100 New-embedded undecalcified bone sections" - Eur Cell Mater. 2003 Dec 31;6:57-71; discussion 71 . But I have yet to actually begin infiltrating the bones. If anyone can offer any knowledge and advice I would greatly appreciate it. Thanks, Kevin Kevin Egan Research Specialist Department of Medicine Division of Geriatric Medicine University of Pennsylvania School of Medicine Biomedical Research Building (BRB) II/III, Room 745 421 Curie Boulevard Philadelphia, PA 19104-6160 Telephone: 215-573-4005 Message: 1 Date: Wed, 28 Jan 2009 17:30:47 -0500 From: "Armando Tellez" Subject: [Histonet] Plastic embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, Does anyone have a protocol for plastic embedding that shows in detail the procedure and equipment needed for this purpose that does not mind share it? My office email is attached to my signature. Please let me know since my boss want to start a stent pathology protocls and he claims that we have the equipment, so first I need a good full blown descriptive protocol and all the protocols in internet are already assuming that you have some plastic embedding knowledge and I have never embed any metal devices. (I need a protocol for microtome, not grinding) Thanks Armando Tellez atellez@crf.org Pathology Department Tel: (845) 290 8034 Fax: (845) 290 8030 From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 29 13:14:09 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 29 13:14:21 2009 Subject: [Histonet] RE: Histonet Digest, Vol 62, Issue 36 In-Reply-To: <01N4V8F97ORY0012Z1@Dino.HealthPartners.Com> References: <01N4V8F97ORY0012Z1@Dino.HealthPartners.Com> Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635D10@hpes1.HealthPartners.int> The sending of this issue only made it through #8..where did the rest go?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 29, 2009 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 62, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Plastic embedding (Armando Tellez) 2. Need any info for JUNG HISTOSTAINER Ig (Michelle MacVeigh-Aloni) 3. MAD2 Immunocytochemistry (Yobou IG) 4. Re: Histonet Digest, Vol 62, Issue 35 (Marilyn.A.Weiss@kp.org) 5. Recycled solvents and chatter (Dunn, Cyndy) 6. 2nd patient identifier on cassettes (Fye Beth) 7. RE: Plastic embedding (Molinari, Betsy) 8. RE: nuclear counterstain (Swain, Frances L) 9. Patient identifier (Webb, Dorothy L) 10. Klotskin's modification of the Masson's Trichrome (Pat Laurie) 11. Re: Klotskin's modification of the Masson's Trichrome (koellingr@comcast.net) 12. FW: [Histonet] Klotskin's modification of the Masson's Trichrome (Lester Raff MD) 13. Re: FW: [Histonet] Klotskin's modification of the Masson's Trichrome (Pat Laurie) 14. Re: FW: [Histonet] Klotskin's modification of the Masson's Trichrome (Bryan Llewellyn) 15. PIN4 Control (thisisann@aol.com) 16. Re: PIN4 Control (Rene J Buesa) 17. quiq decalcifier (Ben Spirto) 18. Georgia Society for Histotechnology Program (Shirley Powell) ---------------------------------------------------------------------- Message: 1 Date: Wed, 28 Jan 2009 17:30:47 -0500 From: "Armando Tellez" Subject: [Histonet] Plastic embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, Does anyone have a protocol for plastic embedding that shows in detail the procedure and equipment needed for this purpose that does not mind share it? My office email is attached to my signature. Please let me know since my boss want to start a stent pathology protocls and he claims that we have the equipment, so first I need a good full blown descriptive protocol and all the protocols in internet are already assuming that you have some plastic embedding knowledge and I have never embed any metal devices. (I need a protocol for microtome, not grinding) Thanks Armando Tellez atellez@crf.org Pathology Department Tel: (845) 290 8034 Fax: (845) 290 8030 ------------------------------ Message: 2 Date: Wed, 28 Jan 2009 17:38:13 -0800 From: Michelle MacVeigh-Aloni Subject: [Histonet] Need any info for JUNG HISTOSTAINER Ig To: histonet@lists.utsouthwestern.edu Message-ID: <001301c981b2$40370840$5c237d80@DFS66DD1> Content-Type: text/plain; charset=iso-8859-1 Hi all, We do research. We got a hand me down JUNG AUTOSTAINER XL which now does all the H&E and it is a blessing! Now I am thinking about purchasing a second hand Immunostainer to run some immunos, so we can use our time for something better than babysitting the slides. There is a very inexpensive JUNG HISTOSTAINER Ig available, but I have no experience in this area. (I couldn't even find a picture of it on the Internet...) For now we only do a TUNEL stain for apoptosis, but the need for more immunos is rising. (Our students use a lot of fluorescent markers if this makes any difference.) What do you think? Do you recommend it or am I just investing in a pricey incubation chamber? I would appreciate any input Michelle ------------------------------ Message: 3 Date: Thu, 29 Jan 2009 03:27:17 -0800 (PST) From: Yobou IG Subject: [Histonet] MAD2 Immunocytochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <989360.3605.qm@web58505.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear All I am trying to detect kinetochore localization of MAD2 induced by a microtubule depolymerizing agent in SW480 human cancer cells. I have used 2 different protocols (-20 oC 100% methanol fixation for 20 minutes or 4% formaldehyde fixation for 20 minutes, both followed by permeabilization with either 0.3% triton/PBS or 100 micog/mL digitonin/PBS) but unfortunately I got negative results. I had success with microtubules immunocytochemical staining using methanol fixation method and I demonstrated the induction of prometaphase arrest by my treament, but it seems that MAD2 immunostaining is not as easy as microtubules. I would greatly appreciate if anyone has an experience in MAD2 immunocytochemical staining provides me with a help. thanks a lot Regards, Yobou I.G., M.Sc. PhD graduate student kyoto prefectural university of medicine Japan ------------------------------ Message: 4 Date: Thu, 29 Jan 2009 04:34:49 -0800 From: Marilyn.A.Weiss@kp.org Subject: [Histonet] Re: Histonet Digest, Vol 62, Issue 35 To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Please take off my previous posting of looking for Pathology Assistant's and post that Kaiser is looking for 2 Pathologist's Assistant's. Send resume to Diane.I.Johnson@kp.org or call 619-528-5386. I would appreciate that. Was in a hurry and wanted to help out my boss, so sorry for the confusion. I certainly know the difference. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ------------------------------ Message: 5 Date: Thu, 29 Jan 2009 07:56:16 -0500 From: "Dunn, Cyndy" Subject: [Histonet] Recycled solvents and chatter To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2009D9CA27C1B8418B551D309223B74902617CFD@mshmail16> Content-Type: text/plain; charset="us-ascii" Does anyone experience chatter they think originates from using recycled solvents in their tissue processors? We have tried many things to eliminate the chatter. The latest was removing the three recycled xylenes from the processor and replacing them with fresh Commercial xylene. After doing this the Pathologist was very pleased with the sections, no chatter in eight blocks, three levels each. ------------------------------ Message: 6 Date: Thu, 29 Jan 2009 07:47:20 -0600 From: Fye Beth Subject: [Histonet] 2nd patient identifier on cassettes To: "histonet@lists.utsouthwestern.edu" Message-ID: <938F8EC5A524D34EB5796E23E52781D3039F603529@NADCWPMSGCMS05.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We average over 400 cassettes a day, and put the Accession # on the top of the cassette, the last name on one side and tissue type on the other side. We are currently still doing all this by hand, but are looking into getting slide and cassette labelers. We started doing this as a matter of safety and not by any regulatory standard 5-6 years ago. It is a procedure that has saved us many times over the years. It is very rare to have cassettes that are mislabeled by both the accession # and patients name. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 ------------------------------ Message: 7 Date: Thu, 29 Jan 2009 07:49:48 -0600 From: "Molinari, Betsy" Subject: RE: [Histonet] Plastic embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" You and the rest of the world Armando. Information like this is costly and hard to come by. Best of luck none the less. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77225 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Armando Tellez Sent: Wednesday, January 28, 2009 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic embedding Hi, Does anyone have a protocol for plastic embedding that shows in detail the procedure and equipment needed for this purpose that does not mind share it? My office email is attached to my signature. Please let me know since my boss want to start a stent pathology protocls and he claims that we have the equipment, so first I need a good full blown descriptive protocol and all the protocols in internet are already assuming that you have some plastic embedding knowledge and I have never embed any metal devices. (I need a protocol for microtome, not grinding) Thanks Armando Tellez atellez@crf.org Pathology Department Tel: (845) 290 8034 Fax: (845) 290 8030 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 29 Jan 2009 08:02:20 -0600 From: "Swain, Frances L" Subject: RE: [Histonet] nuclear counterstain To: "rjbuesa@yahoo.com" , "histonet@lists.utsouthwestern.edu" , "Melissa Mazan" Message-ID: <5B6165D78AC14544974A844787B47E380703CCEF@MAIL5.ad.uams.edu> Content-Type: text/plain; charset=iso-8859-1 Hi Melissa: When definition is compromised I use a 1% light green counterstain for 30 seconds. My boss thinks it is great. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 28, 2009 3:52 PM To: histonet@lists.utsouthwestern.edu; Melissa Mazan Subject: Re: [Histonet] nuclear counterstain If you obtain the detail you need with the DAB, eliminate the counter stain altogether because really you do not need it. Ren? J. --- On Wed, 1/28/09, Melissa Mazan wrote: From: Melissa Mazan Subject: [Histonet] nuclear counterstain To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 28, 2009, 3:11 PM Hi all, I'm staining mouse lung tissues with Dako's Ki67 using Vector ABC Elite and DAB to visualize - I get lovely sharp black brown nuclei where appropriate. There is almost no background staining. However, no matter how lightly I counterstain, I lose the definition between DAB and counterstain - I have tried hematoxylin and Nuclear Red - any suggestions? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Wax. (Ian Montgomery) > 2. Green counterstain Issues (Breeden, Sara) > 3. Re: Green counterstain Issues (Rene J Buesa) > 4. pathology assistants (Marilyn.A.Weiss@kp.org) > 5. RE: pathology assistants (Charles.Embrey) > 6. second patient identifier on tissue cassette (VOGT, LORI) > 7. Re: second patient identifier on tissue cassette (Piero Nelva) > 8. RE: Wax. (Jim Reilly) > 9. RE: pathology assistants (Marilyn.A.Weiss@kp.org) > 10. RE: second patient identifier on tissue cassette (Mike Pence) > 11. RE: second patient identifier on tissue cassette (VOGT, LORI) > 12. RE: pathology assistants (Kimberly Tuttle) > 13. NSH 2009 Winter Lunch and Learn (Brenda Royce) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Jan 2009 18:14:14 -0000 > From: "Ian Montgomery" > Subject: [Histonet] Wax. > To: > Message-ID: <28401324DEB141E78BD380083E48CDEA@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Paraffin wax is giving a few problems with some tissues. What is > the current favourite from UK suppliers? > > Ian. > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Jan 2009 12:26:49 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Green counterstain Issues > To: > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B017E666E@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Jan 2009 12:46:17 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu, "Breeden, Sara" > > Message-ID: <132392.68543.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than adequate time in the fixative. Increase the fixing time and most likely your staining unevenness will disappear. > Ren? J. > > --- On Tue, 1/27/09, Breeden, Sara wrote: > > From: Breeden, Sara > Subject: [Histonet] Green counterstain Issues > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, January 27, 2009, 2:26 PM > > My (working) light green counterstain (Sheehan, Theory & Practice..., pg > 187) does its thing unevenly. Given, a lot of our tissue is poorly > fixed to begin with or possibly autolytic, but I get more staining > around the periphery and little or no staining in the body of the > section. What am I missing here? Thanks! > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Jan 2009 13:31:58 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] pathology assistants > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > ------------------------------ > > Message: 5 > Date: Tue, 27 Jan 2009 16:44:45 -0600 > From: "Charles.Embrey" > Subject: RE: [Histonet] pathology assistants > To: , > Message-ID: > <44780C571F28624DBB446DE55C4D733A1FE637@EXCHANGEBE1.carle.com> > Content-Type: text/plain; charset="us-ascii" > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 27 Jan 2009 19:54:27 -0500 > From: "VOGT, LORI" > Subject: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > > > ------------------------------ > > Message: 7 > Date: Wed, 28 Jan 2009 14:02:06 +1100 > From: "Piero Nelva" > Subject: Re: [Histonet] second patient identifier on tissue cassette > To: > Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F@pentium4> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Before we had our cassette labeller, we wrote the first three letters only of the surname on the side. Pretty awkward, but we had no other option. > > Piero Nelva > Anatomical PAthology > Monash Medical Centre > Australia > > > ----- Original Message ----- From: "VOGT, LORI" > To: > Sent: Wednesday, January 28, 2009 11:54 AM > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on the tissue cassette if you do not have a cassette printer/labeler? Is there an easier way then hand writing patient name on cassette? > > ***************************************************************************************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide personal, high quality, economical health care that meets the needs of our communities. > ***************************************************************************************** > The information contained in the electronic message is privileged and confidential information intended for the use of the addressee listed above. If you are neither the intended recipient or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this telecopied information is strictly prohibited. If you receive this e-mail in error, please immediately notify us by telephone to arrange for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------------- > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.176 / Virus Database: 270.10.14/1920 - Release Date: 1/27/2009 6:15 PM > > > > > ------------------------------ > > Message: 8 > Date: Wed, 28 Jan 2009 09:49:06 -0000 > From: "Jim Reilly" > Subject: RE: [Histonet] Wax. > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello Ian > > In the GBRC we are using Histoplast PE from Thermo Scientific. > > Jim > > James H Reilly Sir Graeme Davies Building > GBRC > 120 university Place > Glasgow > G12 8TA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian > Montgomery > Sent: 27 January 2009 18:14 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax. > > > Paraffin wax is giving a few problems with some tissues. > What is the current favourite from UK suppliers? > > Ian. > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 28 Jan 2009 05:06:22 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: RE: [Histonet] pathology assistants > To: Charles.Embrey@carle.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > 2 assistants. > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > > > "Charles.Embrey" 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Wed, 28 Jan 2009 08:20:31 -0600 > From: "Mike Pence" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "VOGT, LORI" , > > Message-ID: > <661949901A768E4F9CC16D8AF8F2838C017A3A63@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 28 Jan 2009 09:45:14 -0500 > From: "VOGT, LORI" > Subject: RE: [Histonet] second patient identifier on tissue cassette > To: "Mike Pence" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I know it is not a Cap requirement yet. JACHO is requiring 2 patient identifier on specimens and I am looking to the future trickle down effect. Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps. > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Wednesday, January 28, 2009 9:21 AM > To: VOGT, LORI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] second patient identifier on tissue cassette > > > Where in the CAP Standard check list for Anatomic Pathology does it > state that a second patient identifier must be on the tissue cassette? > > I don't remember reading that. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VOGT, > LORI > Sent: Tuesday, January 27, 2009 6:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] second patient identifier on tissue cassette > > > How are you small hospitals dealing with a second patient identifier on > the tissue cassette if you do not have a cassette printer/labeler? Is > there an easier way then hand writing patient name on cassette? > > ************************************************************************ > ***************** > Lori A. Vogt > Technical Section Head/ Histology > Lewistown Hospital > 400 Highland Ave. > Lewistown, PA 17044 > Phone-717-242-7213 > Fax-717-242-7540 > Web Site: http://www.lewistownhospital.org > > The Mission of the Lewistown Healthcare Foundation (LHF) is to provide > personal, high quality, economical health care that meets the needs of > our communities. > ************************************************************************ > ***************** > The information contained in the electronic message is privileged and > confidential information intended for the use of the addressee listed > above. If you are neither the intended recipient or the employee or > agent responsible for delivering this information to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution, or taking of any action in reliance on the content of this > telecopied information is strictly prohibited. If you receive this > e-mail in error, please immediately notify us by telephone to arrange > for return of the original document. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 28 Jan 2009 10:27:31 -0500 > From: "Kimberly Tuttle" > Subject: RE: [Histonet] pathology assistants > To: , > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <498033130200001A00028F75@GWIA2.umm.edu> > Content-Type: text/plain; charset=US-ASCII > > LOL! You didnt answer the question and theres a Big Difference > > > >>>> 01/28/09 8:09 AM >>> >>>> > 2 assistants. > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > > > > > "Charles.Embrey" 01/27/2009 02:44 PM > > To > Marilyn A Weiss/CA/KAIPERM@KAIPERM, > cc > > Subject > RE: [Histonet] pathology assistants > > > > > > > Are the looking for Pathology Assistants or Pathologists' Assistants? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn.A.Weiss@kp.org > Sent: Tuesday, January 27, 2009 3:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] pathology assistants > > Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a > > salaried position. They are a Monday-Friday position from 8:30-5. Great benefits.If you have any questions or would like to send your resume, please contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson@kp.org or 619-528-5386. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > > > ------------------------------ > > Message: 13 > Date: Wed, 28 Jan 2009 11:43:53 -0500 > From: "Brenda Royce" > Subject: [Histonet] NSH 2009 Winter Lunch and Learn > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Registration is now open for the first NSH Winter Lunch & Learn, > presented in partnership with Leica Microsystems, taking place February > 28, 2009 at Jacksonville Community College in Jacksonville, FL. > Attendees have an opportunity to attend 3 sessions and earn 4.5 contact > hours in this half day program. > Schedule: > 8:00am - 9:00am: Registration 9:00am - 10:30am: Small Specimen Management - In a Large Volume World > 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer > 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of Change in Histotechnology > All sessions are presented by top rated NSH presenters Skip Brown, Leica > BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems. > Its a great inexpensive way to expand your knowledge & earn those needed > contact hours. > Visit our website to: www.nsh.org > > Register Online > vt_key=6c37f294-9716-4f7c-a65e-4b7b89435387&RegPath=EventRegFees> or > Download a PDF of the Registration Form > > We hope to see you there! > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 62, Issue 34 > **************************************** > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cummings School of Veterinary Medicine 200 Westborough Road North Grafton,MA 01536 tel: 508-839-5395 fax: 508-839-7903 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Thu, 29 Jan 2009 08:07:35 -0600 From: "Webb, Dorothy L" Subject: [Histonet] Patient identifier To: histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635D04@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" According to the "rule" regarding 2 patient identifiers, it is our understanding that the 2 identifiers must be on the container label and in the computer system upon accessioning. When the computer assigns the surgical case number, all info is linked to that listed surgical accessioned number and your 2 or more identifiers are pulled up with the case. That also keeps within "HIPPA" guidelines to have patient information openly available when blocks or slides are sent out or left out in view. Otherwise, barcoding is the ideal way to place, view, and tie all information needed!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 10 Date: Thu, 29 Jan 2009 06:21:07 -0800 From: Pat Laurie Subject: [Histonet] Klotskin's modification of the Masson's Trichrome To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone, Has anyone heard of the Klotskin's modification of the Masson's Trichrome? I am working with a liver pathologist who wants me to try to work it up. I can't seem to find it anywhere. I have tried numerous books, and scoured the internet. Someone asked about 3 years ago on histonet, but there were no replies. If anyone could help me I would appreciate it. Thanks, -- Patrick Laurie (HT, ASCP) Cellnetix Pathology and Laboratories Seattle, Wa ------------------------------ Message: 11 Date: Thu, 29 Jan 2009 14:31:53 +0000 From: koellingr@comcast.net Subject: Re: [Histonet] Klotskin's modification of the Masson's Trichrome To: Pat Laurie , histonet@lists.utsouthwestern.edu Message-ID: <012920091431.24615.4981BDD90001504B0000602722070229339D09020704040A0105@comcast.net> Isn't a Klatskin tumor (of the biliary tree) spelled with A and not O? There are multiple pictures and references to Klatskin's Trichrome. Try A spelling and not O. Ray Koelling Research Pathology PhenoPath Labs Seattle -------------- Original message ---------------------- From: Pat Laurie > Hi Everyone, > > Has anyone heard of the Klotskin's modification of the Masson's Trichrome? > I am working with a liver pathologist who wants me to try to work it up. I > can't seem to find it anywhere. I have tried numerous books, and scoured > the internet. Someone asked about 3 years ago on histonet, but there were > no replies. If anyone could help me I would appreciate it. > > Thanks, > > -- > Patrick Laurie (HT, ASCP) > Cellnetix Pathology and Laboratories > Seattle, Wa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 29 Jan 2009 08:32:30 -0600 From: "Lester Raff MD" Subject: FW: [Histonet] Klotskin's modification of the Masson's Trichrome To: Message-ID: Content-Type: text/plain; charset="us-ascii" Try searching Klatskin Trichrome. Lots of references on internet. Klatskin was a famous liver pathologist. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Thursday, January 29, 2009 8:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Klotskin's modification of the Masson's Trichrome Hi Everyone, Has anyone heard of the Klotskin's modification of the Masson's Trichrome? I am working with a liver pathologist who wants me to try to work it up. I can't seem to find it anywhere. I have tried numerous books, and scoured the internet. Someone asked about 3 years ago on histonet, but there were no replies. If anyone could help me I would appreciate it. Thanks, -- Patrick Laurie (HT, ASCP) Cellnetix Pathology and Laboratories Seattle, Wa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 29 Jan 2009 07:10:25 -0800 From: Pat Laurie Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's Trichrome To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I apologize for the typo, I meant Klatskin, It is very early in the morning here...There are many references, and yes, a couple of photos of the stain, but no actual procedure... On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD wrote: > Try searching Klatskin Trichrome. Lots of references on internet. > Klatskin was a famous liver pathologist. > > > > Lester J. Raff, MD > > Medical Director > > UroPartners Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel 708.486.0076 > > Fax 708.486.0080 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat > Laurie > Sent: Thursday, January 29, 2009 8:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Klotskin's modification of the Masson's Trichrome > > > > Hi Everyone, > > > > Has anyone heard of the Klotskin's modification of the Masson's > Trichrome? > > I am working with a liver pathologist who wants me to try to work it up. > I > > can't seem to find it anywhere. I have tried numerous books, and > scoured > > the internet. Someone asked about 3 years ago on histonet, but there > were > > no replies. If anyone could help me I would appreciate it. > > > > Thanks, > > > > -- > > Patrick Laurie (HT, ASCP) > > Cellnetix Pathology and Laboratories > > Seattle, Wa > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie (HT, ASCP) Virginia Mason Medical Center Seattle, Wa ------------------------------ Message: 14 Date: Thu, 29 Jan 2009 08:07:43 -0800 From: "Bryan Llewellyn" Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's Trichrome To: "Histonet" , "Pat Laurie" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Klatskin's Modification of Masson Trichrome Stain 1. Hydrate paraffin sections 2. Harris's hematoxylin, 10 minutes 3. 95% ethanol, rinse parent hepatic fat fraction provides an index that can be 4. Picric alcohol (saturated solution of picric acid in 95% ethanol), 10 minutes 5. Wash, 10 minutes 6. Ponceau (1% ponceau in 1% glacial acetic acid), 5 minutes 7. Phosphomolybdic acid (1%, aqueous), 5 minutes 8. Aniline blue (saturated solution of aniline blue in 2.5% glacial acetic acid), 2 minutes 9. Phosphomolybdic acid, 5 minutes 10. 1% acetic acid, 5 minutes 11. 70% alcohol, 2 minutes 12. Dehydrate and mount http://www3.interscience.wiley.com/cgi-bin/fulltext/106594374/PDFSTART Bryan Llewewllyn ----- Original Message ----- From: "Pat Laurie" To: Sent: Thursday, January 29, 2009 7:10 AM Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's Trichrome >I apologize for the typo, I meant Klatskin, It is very early in the morning > here...There are many references, and yes, a couple of photos of the > stain, > but no actual procedure... > > On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD > wrote: > >> Try searching Klatskin Trichrome. Lots of references on internet. >> Klatskin was a famous liver pathologist. >> >> >> >> Lester J. Raff, MD >> >> Medical Director >> >> UroPartners Laboratory >> >> 2225 Enterprise Dr. Suite 2511 >> >> Westchester, Il 60154 >> >> Tel 708.486.0076 >> >> Fax 708.486.0080 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat >> Laurie >> Sent: Thursday, January 29, 2009 8:21 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Klotskin's modification of the Masson's Trichrome >> >> >> >> Hi Everyone, >> >> >> >> Has anyone heard of the Klotskin's modification of the Masson's >> Trichrome? >> >> I am working with a liver pathologist who wants me to try to work it up. >> I >> >> can't seem to find it anywhere. I have tried numerous books, and >> scoured >> >> the internet. Someone asked about 3 years ago on histonet, but there >> were >> >> no replies. If anyone could help me I would appreciate it. >> >> >> >> Thanks, >> >> >> >> -- >> >> Patrick Laurie (HT, ASCP) >> >> Cellnetix Pathology and Laboratories >> >> Seattle, Wa >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Patrick Laurie (HT, ASCP) > Virginia Mason Medical Center > Seattle, Wa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 29 Jan 2009 11:15:41 -0500 From: thisisann@aol.com Subject: [Histonet] PIN4 Control To: histonet@lists.utsouthwestern.edu Message-ID: <8CB5043F733D762-1004-801@FWM-M26.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can someone let me know where I can purchase a good PIN4 control....also, what tissue do you use for the negative when you use one control for multiple cases. Thanks, Ann ------------------------------ Message: 16 Date: Thu, 29 Jan 2009 09:03:57 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] PIN4 Control To: histonet@lists.utsouthwestern.edu, thisisann@aol.com Message-ID: <894423.77079.qm@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Each case ought to have its own negative control. If several cases are treated by the same antibody you can have one single positive control for that Ab, but the same does not apply to negative controls. ren? J. --- On Thu, 1/29/09, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] PIN4 Control To: histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 11:15 AM Can someone let me know where I can purchase a good PIN4 control....also, what tissue do you use for the negative when you use one control for multiple cases. Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 29 Jan 2009 11:20:00 -0600 From: Ben Spirto Subject: [Histonet] quiq decalcifier To: Histonet@lists.utsouthwestern.edu Message-ID: <287d127c0901290920o6160fda1y7e277aac7adb4a6f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Could someone tell me the best way for quick decalcification of parafin embeded brain tissue. I know about treatment with HCl. Anything else? With something, perhaps that can be found in the biochemical lab??? thanks Ben ------------------------------ Message: 18 Date: Thu, 29 Jan 2009 12:18:24 -0500 From: Shirley Powell Subject: [Histonet] Georgia Society for Histotechnology Program To: histonet@lists.utsouthwestern.edu Message-ID: <21B2510769024081812D5D3A12987F77@powellsa1> Content-Type: text/plain; charset=us-ascii The Georgia Society for Histotechnology invites you to the 2009 meeting to be held at Sea Palms Resort at St. Simons Island, Georgia, March 20-22, 2009. The program is below and the registration form can be downloaded from www.histosearch.com/gsh soon or I will email you a copy personally. The complete hotel information is already on the GSH website. Please call for reservations now by calling the Sea Palms Resort. Be sure to tell them you are attending the GSH meeting. The phone number is 1-800-841-6268. Visit the web site at www.seapalms.com. Special GSH Room Rates are $99 for two double beds and $109 for two double beds or a King bed Suites are available as well as Villas. March 20, 2009 - Friday 1 to 5 p.m.: HT/HTL Review Session for Students 5 to 7 p.m.: Meeting Registration 7 to 9 p.m.: Vendor Reception in Vendor Area March 21, 2009 - Saturday 7:00-8:00 a.m.: Meeting Registration 8:30 a.m. to 12: Workshop #1 - Today's Artifacts - Tomorrow's Facts 8:30 a.m. to 12: Workshop #2 - Expense Analysis and Reduction in the IHC Lab (10:00 - 10:30 a.m.: Break in Vendor Area) 12:00 - 1:00-GSH AWARDS LUNCHEON 1:00 to 4:30 p.m.: Workshop #3 - Decalcified and Undecalcified Bone: Histology Techniques 1:00 to 4:30 p.m.: Workshop #4 - Basic Troubleshooting for Histology Laboratory Equipment (2:30 - 3:00 p.m.: Break in Vendor area) 4:30 p.m.: GSH General Membership Meeting (GSH Board Meeting to Follow) March 22, 2009 - Sunday 7:00-8:00 a.m.: Meeting Registration 8:30 to 12 a.m.: Workshop #5 - Commitment in the Workplace - What Does it Mean to the Employee and the Employer 8:30 to 12 a.m.: Workshop #6 - Contemporary Trends in Immunohistochemistry (10:00 - 10:30 a.m.: Break in Vendor Area) WORKSHOP DESCRIPTIONS: #1: Today's Artifacts - Tomorrow's Facts? Lamar Jones, BS, HT(ASCP) - This workshop will teach the participant to recognize and identify artifacts from the gross board, fixation, processing, embedding, microtomy, staining, coverslipping and other areas of histotechnology. #2: Expense Analysis and Reduction in the IHC Lab Joe Myers, - This workshop will discuss ways to cut expenses in the immunohistochemistry laboratory without sacrificing quality. #3: Decalcified and Undecalcified Bone: Histology Techniques Vickie Kalscheur - The speaker will give an overview of Decalcified and Undecalcified bone sample preparation for research histology. Specimen collection, fixation, decalcification, processing, and more will be covered using a wide variety of bone samples. The undecalcified component, again discusses handling, preparation, and staining of undecalcified Plastic embedded bone samples. Special and immunohistochemical staining of bone specimens will be discussed. This talk will be casual and informative for those working in clinical or research settings. Handouts will be provided. #4: Basic Troubleshooting for Histology Laboratory Equipment Jason Velasquez, Technical Engineer -This course will provide a basic preventive maintenance guide that will assist users of histology equipment in the upkeep and troubleshooting of their instruments. The type of cleaning solvents that can and cannot be used will be discussed (along with some pictures that show what happens when the wrong cleaning supplies are used) and how and where to clean for best results. The types of tools that should be kept in the laboratory's tool chest and how and when to use them will be demonstrated. Common types of faults that can be reasonably repaired by the average Histotech will be discussed and the ways, tools and thoughts behind the troubleshooting process will be investigated. Some symptoms that precede failures will be made known so that the users can notify their bio-medical technicians or repair group of a pending failure, before the instrument breaks completely. #5: Commitment in the Workplace - What Does it Mean to the Employee and Employer Wanda Grace Jones, - Hospitals, Research Labs, and Private Laboratories still struggle with continuous loss of employees and finding new employees to fill these positions. Past research has isolated two variables that impact employee turnover. 1st variable is employee's identification with and involvement in an organization (how involved are you). 2nd variable is the employee's perception of level of commitment an organization has to the employee. We will discuss the attitude toward an organization which attaches the person to an organization, the process by which the goals of the organization and employee become integrated, building better communication between employee and employer and cost associated when an employee decides to leave. #6: Contemporary Trends in Immunohistochemistry Mary Cheles, MPH, HTL, DLM(ASCP) - The analysis of a patient has historically relied on morphology and the evaluation of individual antibodies on pathological tissue. Immunohistochemistry has been in practice for the past 40 years. During that time, we have seen an evolution from individual reagents to optimized systems and from manual staining practices to fully automated options. Pathology and laboratory medicine is changing faster than ever. In the future, personalized medicine will define the effect of a therapy based on an individual's gene and protein profile. What does this mean and where does the histology community fit in? This workshop will briefly review immunohistochemistry basics, opportunities for automation, process standardization, antibody validation, regulatory product labeling and current proficiency testing. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 Ph: 478-301-2374 Fx: 478-301-5489 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 36 **************************************** ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From mwich <@t> 7thwavelabs.com Thu Jan 29 13:32:12 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Jan 29 13:32:20 2009 Subject: [Histonet] time guidelines for grossing tissue Message-ID: <62A8156F8071C8439080D626DF8C33A65D8B48@wave-mail.7thwave.local> Does anyone know if there is a "Standard" for times when grossing tissue? I know it takes longer to gross a Kidney than say a Skin Tag ... but is there a general guideline that has been determined for grossing tissue? Thanks in advance for any responses. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From rosenfeldtek <@t> hotmail.com Thu Jan 29 14:01:28 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Jan 29 14:02:16 2009 Subject: [Histonet] re: plastic embedding In-Reply-To: <490222589.38563981233255837240.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> References: <490222589.38563981233255837240.JavaMail.root@zm-mbx-modv.zimbra.upenn.edu> Message-ID: It's been a while, but I used to do plastic sections with a compound called Immuno-Bed. First we dehydrated tissue through an acetone series. From 100% acetone, tissue was then infiltrated in uncatalyzed Immuno-Bed, for...about an hour for tiny samples....then the tissue was finally transferred to plastic embedding molds and covered with catalyzed immunobed. Heat is released, so it might be a good idea to allow the catalytic hardening process to go overnight at 4 degrees C. The plastic blocks popped out of the flexible plastic molds pretty easily. For sectioning, we prepared our own glass knives--You start with a glass rectangle, cut that into squares, then cut the squares into triangles with very, very sharp edges. As I recall, the diamond-edged cutter used to produce the knives cost about $5,000. The plastic blocks are cut using a special microtome with a chuck just for glass knives. It took some practice, but I was able to produce 1 micron sections pretty easily. The sections DO NOT ribbon. Seems like I used a dumont forceps to apply the sections to a drop of water on a slide, then drained the water from underneath the section with a tissue. Or maybe I just let them dry overnight. Can't remember for sure. The next day, slides with plastic sections were washed three times in acetone, then rehydrated with water, then etched in a NaOH bath for ...hmmm, maybe 10 minutes, rinsed, soaked in PBS, and then put through a standard immunocytochemistry protocol. Sorry about fuzziness of details, but it's been about 17 years since I last did the procedure. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 29 Jan 2009 14:03:57 -0500 > From: kevinpe@mail.med.upenn.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: plastic embedding > > I am in a similar situation as Armando. My PI recently told me to figure out how to do plastic embedding to view calcein/Xylenol orange staining. I've been following a procedure described in " Immunohistochemistry of matrix markers in Technovit 9100 New-embedded undecalcified bone sections" - Eur Cell Mater. 2003 Dec 31;6:57-71; discussion 71 . But I have yet to actually begin infiltrating the bones. If anyone can offer any knowledge and advice I would greatly appreciate it. > > Thanks, > Kevin > > Kevin Egan > Research Specialist > Department of Medicine > Division of Geriatric Medicine > University of Pennsylvania School of Medicine > Biomedical Research Building (BRB) II/III, Room 745 > 421 Curie Boulevard > Philadelphia, PA 19104-6160 > Telephone: 215-573-4005 > > Message: 1 > Date: Wed, 28 Jan 2009 17:30:47 -0500 > From: "Armando Tellez" > Subject: [Histonet] Plastic embedding > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi, > > > > Does anyone have a protocol for plastic embedding that shows in detail > the procedure and equipment needed for this purpose that does not mind > share it? My office email is attached to my signature. Please let me > know since my boss want to start a stent pathology protocls and he > claims that we have the equipment, so first I need a good full blown > descriptive protocol and all the protocols in internet are already > assuming that you have some plastic embedding knowledge and I have never > embed any metal devices. (I need a protocol for microtome, not grinding) > > > > Thanks > > > > Armando Tellez > > atellez@crf.org > > Pathology Department > > Tel: (845) 290 8034 > > Fax: (845) 290 8030 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live?: E-mail. Chat. Share. Get more ways to connect. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_allup_explore_012009 From JonSorenson <@t> chiwest.com Thu Jan 29 14:19:25 2009 From: JonSorenson <@t> chiwest.com (Sorenson, Jon (Nampa)) Date: Thu Jan 29 14:21:10 2009 Subject: [Histonet] Document Control Message-ID: <55F887698A32F54F8918ABC6952E0D9D010A198F@CHIMSX02.CHI.catholichealth.net> Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 From JonSorenson <@t> chiwest.com Thu Jan 29 14:30:21 2009 From: JonSorenson <@t> chiwest.com (Sorenson, Jon (Nampa)) Date: Thu Jan 29 14:30:31 2009 Subject: [Histonet] RE: Procedure for Immunoperoxidase on a Frozen Section References: <20090128215830.F9F40D7S4V@email5.catholichealth.net> Message-ID: <55F887698A32F54F8918ABC6952E0D9D010A1990@CHIMSX02.CHI.catholichealth.net> This is how we handle Frozen sections and touch preps for IHC: Frozen tissue is picked up on "Plus" slides, the sections are air dried for a approximately 1 hour and then fixed in room temperature acetone, and finally air-dried again. These slides can now be used or stored in a freezer for later use. Fine Needle Aspirates (FNA's), Cytospins and Touch Preps These specimens are prepared by Cytology and submitted for staining in 95% ethanol, they are rinsed in TBS and stained routinely. Please Note: Fine Needle Aspirations, Cytospins, touch preps and frozen sections need to have procedures that include protease treatment or antigen retrieval modified so as not to include either, they are unnecessary and will be detrimental to the tissue. Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 ________________________________ From lblazek <@t> digestivespecialists.com Thu Jan 29 14:42:01 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 29 14:39:11 2009 Subject: [Histonet] RE: Document Control In-Reply-To: <55F887698A32F54F8918ABC6952E0D9D010A198F@CHIMSX02.CHI.catholichealth.net> References: <55F887698A32F54F8918ABC6952E0D9D010A198F@CHIMSX02.CHI.catholichealth.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E39086A10F825@IBMB7Exchange.digestivespecialists.com> Jon, I have a spreadsheet that I have all of my procedures listed with a document control number that is also in the footer of the procedure. Like "QC-Histo-01-V1". That shows that it is a Quality Control Procedure for Histology and the procedure number is 1 and it is version 1. I create a hyperlink to the procedure. There is also a column that has the procedure name and a column for the date with the next column for when reviewed. If you'd like I can send you an example of what I've done. I make the on line procedures "read only" so they can't be changed unless they have the appropriate security. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sorenson, Jon (Nampa) Sent: Thursday, January 29, 2009 3:19 PM To: Histo Net Subject: [Histonet] Document Control Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beatrice.Debrosse-Serra <@t> pfizer.com Thu Jan 29 14:44:49 2009 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Thu Jan 29 14:44:56 2009 Subject: [Histonet] Microwave fixation Message-ID: <7B41B921086ADE4186377B8C33F702DE07C535F5@lajamrexm01.amer.pfizer.com> Good afternoon! We are interested in microwave fixation. I would be interested to hear about what kind of lab microwave you are using, how happy you are with it and if you are maybe even willing to share your protocol with us. I am very anxious hearing from you. Thanks, Bea Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 From rjbuesa <@t> yahoo.com Thu Jan 29 14:53:07 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 29 14:53:10 2009 Subject: [Histonet] time guidelines for grossing tissue In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8B48@wave-mail.7thwave.local> Message-ID: <475454.76082.qm@web65705.mail.ac4.yahoo.com> Maybe it is not a "general guideline" but I was able to calculate?an average time for grossing/cassetting ranging from 0.9 to 8.3 (average of 2.7) hours per 100 specimens all depending, as you point out, on the type of specimen. Ren? J. --- On Thu, 1/29/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] time guidelines for grossing tissue To: histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 2:32 PM Does anyone know if there is a "Standard" for times when grossing tissue? I know it takes longer to gross a Kidney than say a Skin Tag ... but is there a general guideline that has been determined for grossing tissue? Thanks in advance for any responses. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu Jan 29 15:01:36 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 29 15:01:40 2009 Subject: [Histonet] Document Control References: <55F887698A32F54F8918ABC6952E0D9D010A198F@CHIMSX02.CHI.catholichealth.net> Message-ID: We use a software program called Q-Pulse, which does everything you list below. Google it and you will find a number of websites to view. We store (and control) all of our test method and equipment use SOPs, equipment maintenance and calibration records, employee training and proficiency records, etc. in this program. Jan Shivers Histology/Immunohistochemistry/EM Section Head UMN Veterinary Diagnostic Laboratory ----- Original Message ----- From: "Sorenson, Jon (Nampa)" To: "Histo Net" Sent: Thursday, January 29, 2009 2:19 PM Subject: [Histonet] Document Control Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jan 29 15:25:42 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jan 29 15:27:20 2009 Subject: [Histonet] Document Control References: <55F887698A32F54F8918ABC6952E0D9D010A198F@CHIMSX02.CHI.catholichealth.net> Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB002C94598@e2k3ms1.urmc-sh.rochester.edu> There is also another system called Qualtrax. www.qualtrax.com. ???????????????????????????????? Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jan Shivers Sent: Thu 1/29/2009 4:01 PM To: histonet; Sorenson, Jon (Nampa) Subject: Re: [Histonet] Document Control We use a software program called Q-Pulse, which does everything you list below. Google it and you will find a number of websites to view. We store (and control) all of our test method and equipment use SOPs, equipment maintenance and calibration records, employee training and proficiency records, etc. in this program. Jan Shivers Histology/Immunohistochemistry/EM Section Head UMN Veterinary Diagnostic Laboratory ----- Original Message ----- From: "Sorenson, Jon (Nampa)" To: "Histo Net" Sent: Thursday, January 29, 2009 2:19 PM Subject: [Histonet] Document Control Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 29 15:32:31 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 29 15:32:54 2009 Subject: [Histonet] Document Control In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B6453CE@chi2k3ms01.columbuschildrens.net> Q-pulse is great; we use it as described by Jan, and also for Incident Reporting; another plus is the software is developed by Scots. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Thursday, January 29, 2009 4:02 PM To: histonet; Sorenson, Jon (Nampa) Subject: Re: [Histonet] Document Control We use a software program called Q-Pulse, which does everything you list below. Google it and you will find a number of websites to view. We store (and control) all of our test method and equipment use SOPs, equipment maintenance and calibration records, employee training and proficiency records, etc. in this program. Jan Shivers Histology/Immunohistochemistry/EM Section Head UMN Veterinary Diagnostic Laboratory ----- Original Message ----- From: "Sorenson, Jon (Nampa)" To: "Histo Net" Sent: Thursday, January 29, 2009 2:19 PM Subject: [Histonet] Document Control Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 29 15:37:00 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 29 15:37:31 2009 Subject: [Histonet] consults with slide preparation Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B6453CF@chi2k3ms01.columbuschildrens.net> Curious to see how others are handling this........... We recently received a consult from another facility and ended up preparing a lot of IHC stains on the referred blocks. One of our pathologists believes the IHC slides should be returned to the original requesting lab. Another couple of pathologists and I feel that the slides should be kept here, as we have some sort of legal responsibility, as the facility that prepared the slides from which our consult report was generated. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jencres <@t> ca.rr.com Thu Jan 29 16:21:36 2009 From: jencres <@t> ca.rr.com (jennifer cresor mike hough) Date: Thu Jan 29 16:21:37 2009 Subject: [Histonet] Derm processing times Message-ID: <594213CB19E64BB0AE532A81D5E01500@jennifercresPC> Hello all, I am looking for some feed back on tissue processing times that have worked well. The processor would be strictly dermatology specimens from small to large. Your input is appreciated. Thank you, Jennifer Cresor jencres@ca.rr.com From RSRICHMOND <@t> aol.com Thu Jan 29 17:14:05 2009 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Jan 29 17:14:10 2009 Subject: [Histonet] Re: time guidelines for grossing tissue Message-ID: Michelle Wich asks: >>Does anyone know if there is a "Standard" for times when grossing tissue? I know it takes longer to gross a Kidney than say a Skin Tag ... but is there a general guideline that has been determined for grossing tissue?<< I've never encountered any, in more than 40 years in surgical pathology practice, working maybe 60 pathology services in my locum tenens career. Bob Richmond Samurai Pathologist Knoxville TN From mari.ann.mailhiot <@t> leica-microsystems.com Thu Jan 29 17:55:50 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Thu Jan 29 17:58:24 2009 Subject: [Histonet] Derm processing times In-Reply-To: <594213CB19E64BB0AE532A81D5E01500@jennifercresPC> Message-ID: Jennifer Processing time for skin can very. It sounds like you are going to process all sizes. The most important issue is always fixation. In most cases that is never a problem with skin. I have a protocol for small pieces one and two milimeters. I also have on for 3 milimeters and above. They have always worked the best for me. I also have used xylene substitutes instead of xylene on my skin protocols. I usually just cut the 95% alcohol down to two bottles Then I could have an extra bottle for xylene subs. I always prefered 3 stations of a substitute, but I do know some people only have two xylene subs. I will attach to the bottom of my email. Best Regards (See attached file: skin Processing Protocol template.doc) Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "jennifer cresor mike hough" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Derm processing times 01/29/2009 04:21 PM Hello all, I am looking for some feed back on tissue processing times that have worked well. The processor would be strictly dermatology specimens from small to large. Your input is appreciated. Thank you, Jennifer Cresor jencres@ca.rr.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Jessica.Vacca <@t> HCAhealthcare.com Fri Jan 30 07:33:31 2009 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Fri Jan 30 07:33:48 2009 Subject: [Histonet] Document Control In-Reply-To: References: <55F887698A32F54F8918ABC6952E0D9D010A198F@CHIMSX02.CHI.catholichealth.net> Message-ID: <938D716CD445614ABBB817517557B6F43F7ACB84@NADCWPMSGCMS09.hca.corpad.net> If you have Outlook-tasks, you can set up reminders and even attach the p&p there as well, for you to review. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Thursday, January 29, 2009 4:02 PM To: histonet; Sorenson, Jon (Nampa) Subject: Re: [Histonet] Document Control We use a software program called Q-Pulse, which does everything you list below. Google it and you will find a number of websites to view. We store (and control) all of our test method and equipment use SOPs, equipment maintenance and calibration records, employee training and proficiency records, etc. in this program. Jan Shivers Histology/Immunohistochemistry/EM Section Head UMN Veterinary Diagnostic Laboratory ----- Original Message ----- From: "Sorenson, Jon (Nampa)" To: "Histo Net" Sent: Thursday, January 29, 2009 2:19 PM Subject: [Histonet] Document Control Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dencrowl <@t> MIT.EDU Fri Jan 30 07:55:49 2009 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Fri Jan 30 07:56:02 2009 Subject: [Histonet] JB4 users Message-ID: <2BE65B19-4499-46E2-8158-D37EF85DE048@mit.edu> We are a research core Histology facility accepting specimens from within the MIT community. One of our researchers is trying to embed mouse femurs in JB4 for us to section on our Thermo Finesse microtome utilizing a tungsten carbide blade. The tissue just crumbles out of the resin and we are not able to pick up any sections. We asked the researcher to infiltrate longer, which she did, but the results are the same. We have sectioned other tissues in JB4 with this instrument, so I do not believe the problem is us or the microtome. Any suggestions would be appreciated. Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From micro <@t> superlink.net Fri Jan 30 08:47:39 2009 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Fri Jan 30 08:47:49 2009 Subject: [Histonet] Safranin O stain in plastic sections Message-ID: <118c01c982e9$b42934c0$ac893cd1@DJ4VDH31> Hello all. Does Safranin O staining work on plastic sections (bone, decalcified)? Any help would be appreciated. Regards, Markus From tbraud <@t> holyredeemer.com Fri Jan 30 08:49:11 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Jan 30 08:49:21 2009 Subject: [Histonet] just some ideas Message-ID: Message: 9 Date: Thu, 29 Jan 2009 13:19:25 -0700 From: "Sorenson, Jon (Nampa)" Subject: [Histonet] Document Control Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center JonSorenson@chiwest.com 208-463-5267 Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. If your procedures are in a word processing program, such as Word, they can be security controlled by user to protect the contents. Any user may view, but as a "read only". 2. An automated review reminder process Through Outlook (if you have it) tasks or Calendar (which you can set pop up reminders). 3. Electronic or online review and/or signature process. As long as you have a procedure that shows the procedure is clearly restricted, and that shows that by adding a signature is also protected and defined as your "electronic signature" then you could type your name and date as review. I move each reviewed procedure into a new folder for that year when electronically signed or revised.(the entire process is clearly defined in the procedure) Example: I have a general lab procedure titled APD-321r2 Proficiency Testing in a "General Procedures" Folder in a folder titled "2008" on a protected drive with write access to the folder limited to myself, the medical director, and lab admin. All technicians have "read only" access to that drive. It is due to be reviewed this month. The r2 following the procedure number is indicative that this is the second revision of this procedure. If I were to revise the procedure during 2008, I would revise, resign, and "Save As" APD-321r3 Proficiency Testing, then move the APD-321r2 into a Retired Procedures folder within the 2008 folder. To Review, I would review, resign on a signature page by typing my name, and move into a folder in "General Procedures" in a folder "2009" on the same protected drive. I don't move retired procedures forward, which allows you to purge retired procedures after the requisite 2 years. I hope this helps. It sounds way more complicated than it is. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From ratliffjack <@t> hotmail.com Fri Jan 30 08:56:23 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Jan 30 08:56:32 2009 Subject: [Histonet] Safranin O stain in plastic sections In-Reply-To: <118c01c982e9$b42934c0$ac893cd1@DJ4VDH31> References: <118c01c982e9$b42934c0$ac893cd1@DJ4VDH31> Message-ID: Yes, it works quite well for both demineralized and undemineralized bone sections embedded in resin. I perform MMA embedding (not a kit) and get really nice undemineralized results. The resin I use allows me the opportunity to deplastify the sections just as one would deparaffinize when working with paraffin. Jack Ratliff On Jan 30, 2009, at 8:47 AM, "Markus F. Meyenhofer" wrote: > Hello all. > Does Safranin O staining work on plastic sections (bone, decalcified)? > Any help would be appreciated. > Regards, > Markus > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carrolpb <@t> umdnj.edu Fri Jan 30 09:22:28 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jan 30 09:25:47 2009 Subject: [Histonet] Safranin O stain in plastic sections In-Reply-To: References: <118c01c982e9$b42934c0$ac893cd1@DJ4VDH31> Message-ID: <49831B34.2000908@umdnj.edu> Gotta second this. I've done a lot of PMMA and/or Spurrs-embedded sectioning with it with good results. Jack Ratliff wrote: > Yes, it works quite well for both demineralized and undemineralized > bone sections embedded in resin. I perform MMA embedding (not a kit) > and get really nice undemineralized results. The resin I use allows me > the opportunity to deplastify the sections just as one would > deparaffinize when working with paraffin. > > Jack Ratliff > > On Jan 30, 2009, at 8:47 AM, "Markus F. Meyenhofer" > wrote: > >> Hello all. >> Does Safranin O staining work on plastic sections (bone, decalcified)? >> Any help would be appreciated. >> Regards, >> Markus >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gu.lang <@t> gmx.at Fri Jan 30 09:28:28 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jan 30 09:28:37 2009 Subject: [Histonet] immunoportal? Message-ID: <2BE20D8D39F64B558B6220BEAA9699B4@dielangs.at> Hi all, has anyone information about the immunoportal website? It always shows errors, when I try to get in. www.immunoportal.com Gudrun From rjbuesa <@t> yahoo.com Fri Jan 30 09:37:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 30 09:37:56 2009 Subject: [Histonet] quiq decalcifier In-Reply-To: <287d127c0901290920o6160fda1y7e277aac7adb4a6f@mail.gmail.com> Message-ID: <456681.24959.qm@web65701.mail.ac4.yahoo.com> Embedded BRAIN tissue decalcifier? Never heard this before! Leave and learn! Ren? J. --- On Thu, 1/29/09, Ben Spirto wrote: From: Ben Spirto Subject: [Histonet] quiq decalcifier To: Histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 12:20 PM Could someone tell me the best way for quick decalcification of parafin embeded brain tissue. I know about treatment with HCl. Anything else? With something, perhaps that can be found in the biochemical lab??? thanks Ben _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Fri Jan 30 09:48:35 2009 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Jan 30 09:48:42 2009 Subject: [Histonet] quiq decalcifier In-Reply-To: <456681.24959.qm@web65701.mail.ac4.yahoo.com> References: <287d127c0901290920o6160fda1y7e277aac7adb4a6f@mail.gmail.com> <456681.24959.qm@web65701.mail.ac4.yahoo.com> Message-ID: Fairly common procedure Rene with the percentage of boneheads in the population. I?ve got kids who use to live in my house who were boneheads. ;-) ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 30, 2009 9:38 AM To: Histonet@lists.utsouthwestern.edu; Ben Spirto Subject: Re: [Histonet] quiq decalcifier Embedded BRAIN tissue decalcifier? Never heard this before! Leave and learn! Ren? J. --- On Thu, 1/29/09, Ben Spirto wrote: From: Ben Spirto Subject: [Histonet] quiq decalcifier To: Histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 12:20 PM Could someone tell me the best way for quick decalcification of parafin embeded brain tissue. I know about treatment with HCl. Anything else? With something, perhaps that can be found in the biochemical lab??? thanks Ben _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jan 30 09:48:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 30 09:48:51 2009 Subject: [Histonet] consults with slide preparation In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21B6453CF@chi2k3ms01.columbuschildrens.net> Message-ID: <916113.7214.qm@web65711.mail.ac4.yahoo.com> He who signed the final diagnosis on this consultation ought to keep the slides because they are the "proof" of such final diagnosis. If you just did the IHC procedures to be sent to them so they would do the final diagnosis, then they keep the slides. Ren? J. --- On Thu, 1/29/09, Houston, Ronald wrote: From: Houston, Ronald Subject: [Histonet] consults with slide preparation To: "histonet" , ihcrg@googlegroups.com Date: Thursday, January 29, 2009, 4:37 PM Curious to see how others are handling this........... We recently received a consult from another facility and ended up preparing a lot of IHC stains on the referred blocks. One of our pathologists believes the IHC slides should be returned to the original requesting lab. Another couple of pathologists and I feel that the slides should be kept here, as we have some sort of legal responsibility, as the facility that prepared the slides from which our consult report was generated. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Jan 30 10:04:59 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jan 30 10:08:13 2009 Subject: [Histonet] quiq decalcifier In-Reply-To: <456681.24959.qm@web65701.mail.ac4.yahoo.com> References: <456681.24959.qm@web65701.mail.ac4.yahoo.com> Message-ID: <4983252B.4030009@umdnj.edu> > Embedded BRAIN tissue decalcifier? You know, I must have forgotten that today is friday, because I really passed up the opportunity to crack some fairly good puns at Ben's inquiry ;) From sheila_adey <@t> hotmail.com Fri Jan 30 11:07:37 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Jan 30 11:07:41 2009 Subject: [Histonet] Who can gross?? Message-ID: Hi All, We are short histo techs and are using aids to help more and more. Our manager believes they can be doing the grossing of a histo tech as long as they have a tech or Dr to consult with. Can someone remind me of the guide lines for grossing. One has a BSc, is that enough? Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/windows/windowslive/messenger.aspx From gayle.callis <@t> bresnan.net Fri Jan 30 10:57:25 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 30 11:12:39 2009 Subject: [Histonet] JB4 users In-Reply-To: <2BE65B19-4499-46E2-8158-D37EF85DE048@mit.edu> References: <2BE65B19-4499-46E2-8158-D37EF85DE048@mit.edu> Message-ID: <000e01c982fb$d374f730$7a5ee590$@callis@bresnan.net> GMA in general, does not infiltrate into calcified mouse femurs as well as methyl methacrylate, and controlling polymerization is not easy with calcified bone in GMA. You have already tried extended infiltrations, but didn't change the results. I suggest you change to a Technovits 9100 kit, and do methyl methacrylate instead. Be sure to allow more time for processing compared to paraffin or even GMA. Your microtome and tungsten carbide blade are not the problem, it is using calcified mouse femurs with GMA. You can remove methyl methacrylate from sections for immunostaining or other stains that will not penetrate this very hydrophobic plastic. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59175 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise Crowley Sent: Friday, January 30, 2009 6:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] JB4 users We are a research core Histology facility accepting specimens from within the MIT community. One of our researchers is trying to embed mouse femurs in JB4 for us to section on our Thermo Finesse microtome utilizing a tungsten carbide blade. The tissue just crumbles out of the resin and we are not able to pick up any sections. We asked the researcher to infiltrate longer, which she did, but the results are the same. We have sectioned other tissues in JB4 with this instrument, so I do not believe the problem is us or the microtome. Any suggestions would be appreciated. Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Jan 30 10:48:33 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 30 11:18:48 2009 Subject: [Histonet] quiq decalcifier In-Reply-To: <456681.24959.qm@web65701.mail.ac4.yahoo.com> References: <287d127c0901290920o6160fda1y7e277aac7adb4a6f@mail.gmail.com> <456681.24959.qm@web65701.mail.ac4.yahoo.com> Message-ID: <000d01c982fa$9665ab10$c3310130$@callis@bresnan.net> Ben, Please clarify what you mean here and what species you are working with. Are you working with mouse heads with skull and whole brain intact? Or are there residual skull fragments or the does the brain contain some calcified areas (latter is something I personally have not experienced or heard of??) Unfortunately, quick decalcification may not be a choice but one can choose a decalcifying agent that is more rapid than others. HCL, commercial products are very rapid while formic acid is generally slower and more gentle. One can adjust acid concentrations to increase speed. If you want a quicker decalcifier, I suggest any rapid HCL available from Thermo, VWR, Surgipath, Richard Allan, or even RDO (very strong and fast HCL decalcifying agent). I am taking some guesses here, but maybe these suggestions are what you need. We have done formalin fixed whole mouse and rat skulls, after removing ears, and skin for 7 to 10 days, then decalcified these with 15% formic acid using an endpoint determination to know when calcium is gone. The paraffin processing schedule was extended considerably. Results were excellent for whole skull 5 um skull sections stained with H&E. We could do cross sections from front of nose to back of skull OR mid sagittal from one side of skull to other, semi-serial sections. The latter gave us the best view of what we were looking for in the nasal turbinates. Although we were more interested in the bone, the brain was well preserved, and well stained. If you have residual calcifications in your embedded sample, simply face the block and put the block face down in a decalcifying solution for 15 minutes or so (formic acid or HCl) then rinse well to get rid of acid, recool block if needed, return to microtome and section. Be sure to NOT retrim the block face, as you need to access the first few sections that come off the block, as block face decalcification only penetrates a few um. Good luck from an Old Bonehead, and hope these SUGGESTIONS help you. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 --- On Thu, 1/29/09, Ben Spirto wrote: From: Ben Spirto Subject: [Histonet] quiq decalcifier To: Histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 12:20 PM Could someone tell me the best way for quick decalcification of parafin embeded brain tissue. I know about treatment with HCl. Anything else? With something, perhaps that can be found in the biochemical lab??? thanks Ben From Charles.Embrey <@t> carle.com Fri Jan 30 11:26:25 2009 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Jan 30 11:27:14 2009 Subject: [Histonet] Who can gross?? In-Reply-To: References: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> As short histo techs what kind of aids are you using, stepladders or stools? Sorry, I couldn't resist. There is a ton of information on the histonet archive about this. A search should yield all the info you seek and more. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Friday, January 30, 2009 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can gross?? Hi All, We are short histo techs and are using aids to help more and more. Our manager believes they can be doing the grossing of a histo tech as long as they have a tech or Dr to consult with. Can someone remind me of the guide lines for grossing. One has a BSc, is that enough? Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/windows/windowslive/messenger.aspx_____________ __________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Fri Jan 30 11:39:50 2009 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Jan 30 11:47:39 2009 Subject: [Histonet] Presenters for HT/HTL Review Session for GSH Message-ID: <5AA4E73828484DCFBCD3E9187CEC7A15@powellsa1> The names of the presenters for the HT/HTL review session was not on the program I first sent out on histonet. The review will be presented by Carl Sagasser, BS,HT(ASCP) Educational Coordinator and Taiquanda Winbush, AS, HT(ASCP) Clinical Laboratory Coordinator with the Darton College Histology Online Program. This is a NACCLS approved histology school in Albany, Georgia. The program can be seen by going to www.darton.edu and going to the online programs, then the histotechnology link. . Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 Ph: 478-301-2374 Fx: 478-301-5489 From juditw <@t> u.washington.edu Fri Jan 30 11:59:29 2009 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Fri Jan 30 11:59:38 2009 Subject: [Histonet] Who can gross?? In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> Message-ID: Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: > As short histo techs what kind of aids are you using, stepladders or > stools? Sorry, I couldn't resist. There is a ton of information on the > histonet archive about this. A search should yield all the info you > seek and more. > > Charles Embrey, PA(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila > adey > Sent: Friday, January 30, 2009 11:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Who can gross?? > > > Hi All, > > We are short histo techs and are using aids to help more and more. Our > manager believes they can be doing the grossing of a histo tech as long > as they have a tech or Dr to consult with. Can someone remind me of the > guide lines for grossing. One has a BSc, is that enough? > > Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan > _________________________________________________________________ > Windows Live Messenger. Multitasking at its finest. > http://www.microsoft.com/windows/windowslive/messenger.aspx_____________ > __________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From Norm.Burnham <@t> propath.com Fri Jan 30 12:09:26 2009 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Jan 30 12:10:11 2009 Subject: [Histonet] Who can gross?? In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> Message-ID: <82C7248978CB50469FD6BA68EBBEFE67F0408E@exchange.propathlab.com> Better sources include: CAP Anatomic Pathology checklist or CLIA regulations -- which are quite specific as to "who can gross". Cheers, Norm Burnham ProPath www.propath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, January 30, 2009 11:26 AM To: sheila adey Cc: histonet Subject: RE: [Histonet] Who can gross?? As short histo techs what kind of aids are you using, stepladders or stools? Sorry, I couldn't resist. There is a ton of information on the histonet archive about this. A search should yield all the info you seek and more. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Friday, January 30, 2009 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can gross?? Hi All, We are short histo techs and are using aids to help more and more. Our manager believes they can be doing the grossing of a histo tech as long as they have a tech or Dr to consult with. Can someone remind me of the guide lines for grossing. One has a BSc, is that enough? Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/windows/windowslive/messenger.aspx_____________ __________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Fri Jan 30 12:12:25 2009 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Jan 30 12:13:08 2009 Subject: [Histonet] Who can gross?? In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE67F0408E@exchange.propathlab.com> References: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> <82C7248978CB50469FD6BA68EBBEFE67F0408E@exchange.propathlab.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE63E@EXCHANGEBE1.carle.com> Those are exactly the sources discussed in the archives. -----Original Message----- From: Norm Burnham [mailto:Norm.Burnham@propath.com] Sent: Friday, January 30, 2009 12:09 PM To: Charles.Embrey; sheila adey Cc: histonet Subject: RE: [Histonet] Who can gross?? Better sources include: CAP Anatomic Pathology checklist or CLIA regulations -- which are quite specific as to "who can gross". Cheers, Norm Burnham ProPath www.propath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, January 30, 2009 11:26 AM To: sheila adey Cc: histonet Subject: RE: [Histonet] Who can gross?? As short histo techs what kind of aids are you using, stepladders or stools? Sorry, I couldn't resist. There is a ton of information on the histonet archive about this. A search should yield all the info you seek and more. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Friday, January 30, 2009 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can gross?? Hi All, We are short histo techs and are using aids to help more and more. Our manager believes they can be doing the grossing of a histo tech as long as they have a tech or Dr to consult with. Can someone remind me of the guide lines for grossing. One has a BSc, is that enough? Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/windows/windowslive/messenger.aspx_____________ __________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Jan 30 12:15:24 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Jan 30 12:16:49 2009 Subject: [Histonet] Who can gross?? In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE63E@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> <82C7248978CB50469FD6BA68EBBEFE67F0408E@exchange.propathlab.com> <44780C571F28624DBB446DE55C4D733A1FE63E@EXCHANGEBE1.carle.com> Message-ID: <498343BC.3030103@umdnj.edu> > Those are exactly the sources discussed in the archives. B-but... why check the archives when we can just ask the entire group the same questions repeatedly? ;-) From froyer <@t> bitstream.net Fri Jan 30 12:49:18 2009 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Jan 30 12:49:27 2009 Subject: [Histonet] Who can gross?? In-Reply-To: References: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> Message-ID: I hear you Judy... you have to pick them up just to say hello! ;-) (Now before there is a flame of political correctness coming back at us... everyone lighten up. I AM one of those short people, and I can make fun of myself if I want too). ;-) Ford M. Royer, MT(ASCP) ...who use to have to sit on a phone book so that I could reach the microscope to scan slides for QA. Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams Sent: Friday, January 30, 2009 11:59 AM To: Charles.Embrey Cc: histonet Subject: RE: [Histonet] Who can gross?? Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: > As short histo techs what kind of aids are you using, stepladders or > stools? Sorry, I couldn't resist. There is a ton of information on the > histonet archive about this. A search should yield all the info you > seek and more. > > Charles Embrey, PA(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila > adey > Sent: Friday, January 30, 2009 11:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Who can gross?? > > > Hi All, > > We are short histo techs and are using aids to help more and more. Our > manager believes they can be doing the grossing of a histo tech as long > as they have a tech or Dr to consult with. Can someone remind me of the > guide lines for grossing. One has a BSc, is that enough? > > Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan > _________________________________________________________________ > Windows Live Messenger. Multitasking at its finest. > http://www.microsoft.com/windows/windowslive/messenger.aspx_____________ > __________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Jan 30 12:52:14 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jan 30 12:52:31 2009 Subject: [Histonet] Who can gross?? In-Reply-To: References: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1333430@LTA3VS011.ees.hhs.gov> Hey, I am one of those "short people" and I do NOT have tiny little teeth OR nasty little feet. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, January 30, 2009 1:49 PM To: 'histonet' Subject: RE: [Histonet] Who can gross?? I hear you Judy... you have to pick them up just to say hello! ;-) (Now before there is a flame of political correctness coming back at us... everyone lighten up. I AM one of those short people, and I can make fun of myself if I want too). ;-) Ford M. Royer, MT(ASCP) ...who use to have to sit on a phone book so that I could reach the microscope to scan slides for QA. Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams Sent: Friday, January 30, 2009 11:59 AM To: Charles.Embrey Cc: histonet Subject: RE: [Histonet] Who can gross?? Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: > As short histo techs what kind of aids are you using, stepladders or > stools? Sorry, I couldn't resist. There is a ton of information on > the histonet archive about this. A search should yield all the info > you seek and more. > > Charles Embrey, PA(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila > adey > Sent: Friday, January 30, 2009 11:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Who can gross?? > > > Hi All, > > We are short histo techs and are using aids to help more and more. Our > manager believes they can be doing the grossing of a histo tech as > long as they have a tech or Dr to consult with. Can someone remind me > of the guide lines for grossing. One has a BSc, is that enough? > > Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan > _________________________________________________________________ > Windows Live Messenger. Multitasking at its finest. > http://www.microsoft.com/windows/windowslive/messenger.aspx___________ > __ > __________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Jan 30 13:13:14 2009 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Jan 30 13:13:18 2009 Subject: [Histonet] JB4 users In-Reply-To: <000e01c982fb$d374f730$7a5ee590$@callis@bresnan.net> References: <2BE65B19-4499-46E2-8158-D37EF85DE048@mit.edu> <000e01c982fb$d374f730$7a5ee590$@callis@bresnan.net> Message-ID: <003101c9830e$cd3b5bd0$095a5b82@vet.upenn.edu> I agree with Gayle. GMA does not work well with mouse femurs. Not only are the issues with infiltration a problem, even when using mixtures of ethanol to pure GMA it still will not penetrate well and polymerizes for to quickly and too hot to make a good block. I personally find it too soft for the calcified femurs to hold in the block well. We don't use a kit and make our own MMA mixture so we can control the softeners better for sectioning and the temperature. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Friday, January 30, 2009 11:57 AM To: 'Denise Crowley'; Histonet Subject: RE: [Histonet] JB4 users GMA in general, does not infiltrate into calcified mouse femurs as well as methyl methacrylate, and controlling polymerization is not easy with calcified bone in GMA. You have already tried extended infiltrations, but didn't change the results. I suggest you change to a Technovits 9100 kit, and do methyl methacrylate instead. Be sure to allow more time for processing compared to paraffin or even GMA. Your microtome and tungsten carbide blade are not the problem, it is using calcified mouse femurs with GMA. You can remove methyl methacrylate from sections for immunostaining or other stains that will not penetrate this very hydrophobic plastic. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59175 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise Crowley Sent: Friday, January 30, 2009 6:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] JB4 users We are a research core Histology facility accepting specimens from within the MIT community. One of our researchers is trying to embed mouse femurs in JB4 for us to section on our Thermo Finesse microtome utilizing a tungsten carbide blade. The tissue just crumbles out of the resin and we are not able to pick up any sections. We asked the researcher to infiltrate longer, which she did, but the results are the same. We have sectioned other tissues in JB4 with this instrument, so I do not believe the problem is us or the microtome. Any suggestions would be appreciated. Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Jan 30 17:19:59 2009 From: jnocito <@t> satx.rr.com (JoeNocito) Date: Fri Jan 30 17:20:18 2009 Subject: [Histonet] Who can gross?? In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1333430@LTA3VS011.ees.hhs.gov> References: <44780C571F28624DBB446DE55C4D733A1FE63D@EXCHANGEBE1.carle.com> <9A16CB5D55FC1648ADF11B63E72A1BE1333430@LTA3VS011.ees.hhs.gov> Message-ID: <003001c98331$46cff600$d46fe200$@rr.com> I'm not short, just vertically challenged JTT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, January 30, 2009 12:52 PM To: Ford Royer; histonet Subject: RE: [Histonet] Who can gross?? Hey, I am one of those "short people" and I do NOT have tiny little teeth OR nasty little feet. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, January 30, 2009 1:49 PM To: 'histonet' Subject: RE: [Histonet] Who can gross?? I hear you Judy... you have to pick them up just to say hello! ;-) (Now before there is a flame of political correctness coming back at us... everyone lighten up. I AM one of those short people, and I can make fun of myself if I want too). ;-) Ford M. Royer, MT(ASCP) ...who use to have to sit on a phone book so that I could reach the microscope to scan slides for QA. Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams Sent: Friday, January 30, 2009 11:59 AM To: Charles.Embrey Cc: histonet Subject: RE: [Histonet] Who can gross?? Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: > As short histo techs what kind of aids are you using, stepladders or > stools? Sorry, I couldn't resist. There is a ton of information on > the histonet archive about this. A search should yield all the info > you seek and more. > > Charles Embrey, PA(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila > adey > Sent: Friday, January 30, 2009 11:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Who can gross?? > > > Hi All, > > We are short histo techs and are using aids to help more and more. Our > manager believes they can be doing the grossing of a histo tech as > long as they have a tech or Dr to consult with. Can someone remind me > of the guide lines for grossing. One has a BSc, is that enough? > > Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan > _________________________________________________________________ > Windows Live Messenger. Multitasking at its finest. > http://www.microsoft.com/windows/windowslive/messenger.aspx___________ > __ > __________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jencres <@t> ca.rr.com Fri Jan 30 17:35:01 2009 From: jencres <@t> ca.rr.com (jennifer cresor mike hough) Date: Fri Jan 30 17:35:04 2009 Subject: [Histonet] Fw: Derm processing times Message-ID: ----- Original Message ----- From: jennifer cresor mike hough To: histonet@lists.utsouthwestern.edu Sent: Thursday, January 29, 2009 2:21 PM Subject: Derm processing times Hello all, I am looking for some feed back on tissue processing times that have worked well. The processor would be strictly dermatology specimens from small to large. Your input is appreciated. Thank you, Jennifer Cresor jencres@ca.rr.com From lchen <@t> mednet.ucla.edu Fri Jan 30 20:30:01 2009 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Fri Jan 30 20:30:05 2009 Subject: [Histonet] cryostat adapter Message-ID: <361429220901301830i55efbcf8p28244d20cceba022@mail.gmail.com> Hello, I have been trying to find a cryostat adapter for 6 months with no luck. Does anyone know where I can find an adapter to hold plastic embedding rings such as this: http://www.emsdiasum.com/microscopy/products/histology/imaages2/62350__.jpg We have a Leica and I've called Leica, called Thermo-Sci, Mikron, Wesco, searched online, and I can't find it anywhere. I know they exist because the last two labs I worked in had these for their machines and none of the reps can tell me how to get one. Thanks! Leslie From Histowa13 <@t> aol.com Sat Jan 31 06:59:05 2009 From: Histowa13 <@t> aol.com (Histowa13@aol.com) Date: Sat Jan 31 07:00:19 2009 Subject: [Histonet] Re: Histonet Digest, Vol 62, Issue 38; IHC slides as consults Message-ID: Hi Ronnie, We keep all the slides that we prepare along with an H&E for our files. We return the block(s) or any unstained slides we may not have used. This way we have material on this case not only from a legal standpoint but in case that would be a case an inspector may randomly ask for. Debbie Nannenga, HTL(ASCP)QIHC InCyte Pathology


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