[Histonet] freezing paraffin blocks

[Rene J Buesa]

Not at all!
René J.

--- On Fri, 2/13/09, Jennifer Anderson <janderson <@t> halozyme.com> wrote:

From: Jennifer Anderson <janderson <@t> halozyme.com>
Subject: [Histonet] freezing paraffin blocks
To: Histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 13, 2009, 5:43 PM

Hi All.

 

I've just started sectioning Bouin's fixed paraffin embedded
human/mouse/rat/pig skin again, tissues processed in a softer wax than
I'm used to, so I've been putting the blocks in the freezer at -20
prior
to sectioning.  Will such cold deleteriously affect the blocks or
tissue?

 

Also, is there any harm in cleaning the water bath with a little xylene
to remove any wax build-up?

 

Thanks a lot.

 

Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11404 Sorrento Valley Road

San Diego, CA 92121

858-704-8333

janderson <@t> halozyme.com

 

 


The information transmitted in this email is confidential and is intended only
for the person(s) or entity to which it is addressed.  Delivery of this message
to any person other than the intended recipient(s) is not intended in any way to
waive confidentiality or any applicable privilege.  Any review, retransmission,
dissemination or other use of, or taking of any action in reliance upon, this
information by individuals or entities other than the intended recipient is
prohibited by Halozyme and may be in violation of applicable laws.  If you
received this in error, please contact the sender and delete/destroy this email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet