[Histonet] Renal Question
Walter Benton
WBENTON <@t> umm.edu
Thu Dec 24 12:14:31 CST 2009
Kim,
In response to your 1st question our Pathologist along with the surgeon
generally look under the scope together at the specimen, never a
histotech.
In response to the 2nd question, regardless of location we do not
utilize the histotech for help. PAs or Residents handle all frozens. Our
true off site cases are sent via image analysis over the internet, which
are read by the Pathologist.
Hope this helps.
Message: 5
Date: Thu, 24 Dec 2009 09:03:58 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: [Histonet] Hello fellow Histo's , 2 questions for you
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFEF9E5F26.5F9AD0CB-ON86257696.005209ED-86257696.0052C2D7 <@t> bhcpns.org>
Content-Type: text/plain; charset="US-ASCII"
First of all I hope all of you are enjoying your Holiday. And I expect
that many of you are not at work like me, but when you get a chance if
you
could help me out by answering these two questions I would be very
grateful. :-)
1) In your lab when you receive renal biopsy's fresh. Who finds the
Glomeruli using a microscope? A pathologist or a Histologist?
2) Also, if you have frozen sections off site, do you always send a
Histologist to assist the Pathologist?
Thanks for your help in advance
Many Blessing to each of you!
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508
>>> On 12/24/2009 at 1:06 PM,
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Today's Topics:
1. Thomas Crowell is out of the office.
(thomas.crowell <@t> novartis.com)
2. Auto Reply (Baustin)
3. IF staining on FFPE tissue (Margaryan, Naira)
4. RE: IF staining on FFPE tissue (Margaryan, Naira)
5. Hello fellow Histo's , 2 questions for you
(Kim.Donadio <@t> bhcpns.org)
6. Hello fellow Histo's , 2 questions for you (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Wed, 23 Dec 2009 13:07:00 -0500
From: thomas.crowell <@t> novartis.com
Subject: [Histonet] Thomas Crowell is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF62B911FD.090A0C3E-ON85257695.00638490-85257695.00638490 <@t> ah.novartis.com>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 12/23/2009 and will not return
until
01/05/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
------------------------------
Message: 2
Date: Wed, 23 Dec 2009 13:10:02 -0500
From: Baustin <baustin <@t> cbgbiotech.com>
Subject: [Histonet] Auto Reply
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <999778625 <@t> mail-server.cbgbiotech.com>
Content-Type: text/plain; charset="utf-8"
Please do not respond to this automatic e-mail reply.
I will be out of the office until Monday, January 4, 2010. If you need
to place an order, please email it to supplies <@t> cbgbiotech.com or fax it
to 614-863-1676 Attn Ordering.
If you need to place a credit card order, please call 1-800-941-9484
and dial ext 201 or 225.
Please note that all orders have been confirmed. If you did not receive
a fax confirmation, your order was not received by CBG.
------------------------------
Message: 3
Date: Wed, 23 Dec 2009 12:55:19 -0600
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] IF staining on FFPE tissue
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1BA93040C6B9A4A8D84878F93FEC36A038F2087BB <@t> CMHEXCC01MBX.childrensmemorial.org>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
Hopefully you are enjoying a great Holidays with your friends and
family!
I have been asked to perform . I got positive and negative absolutely
identical with beautiful nuclear DAPI and very red RBC. What to do or
what to change in my protocol to avoid RBC and background? Here is my
protocol:
1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, 70%-
5min, H2O)
2. Antigen Retrieval ph6 in Citrate buffer (same I use for IHC)
3. Wash H2O and TBST
4. Protein block-
10minhttps://webmail.childrensmemorial.org/owa/?ae=PreFormAction&t=IPM.Note&a=Reply&id=RgAAAADBaOhrx0l9S5XoN3P8OXzpBwDbYZXQd%2bZ%2fQIud7XQY1kjHAAAKzOB%2bAADBupMEDGuaSo2Eh4%2bT%2fsNqAAOO6ORIAAAJ#
5. Ab, Rb anti-human - 60-90 min (same I use for IHC)
6. Wash TBST -5-10min
7. secondary Donkey anti-Rb 594 red - 60min
8. Wash TBST -5-10min, H2O -5min
9. DAPI - 10min
10. Wash H2O -5-10min
11. Gelvatol coverslip
Am I using wrong secondary? Is there any specific secondary or protocol
for IF staining for FFPE to avoid background? I just used same AR and
primary Ab's dilution like i use for IHC with nice results.
Any suggestions are appreciated, especially in these Holidays days from
working histo- people:)
Thanks and have a warm and nice Holidays,
Naira
------------------------------
Message: 4
Date: Wed, 23 Dec 2009 12:56:23 -0600
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] RE: IF staining on FFPE tissue
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1BA93040C6B9A4A8D84878F93FEC36A038F2087BC <@t> CMHEXCC01MBX.childrensmemorial.org>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
Hopefully you are enjoying a great Holidays with your friends and
family!
I have been asked to perform . I got positive and negative absolutely
identical with beautiful nuclear DAPI and very red RBC. What to do or
what to change in my protocol to avoid RBC and background? Here is my
protocol:
1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, 70%-
5min, H2O)
2. Antigen Retrieval ph6 in Citrate buffer (same I use for IHC)
3. Wash H2O and TBST
4. Protein block- 10min
5. Ab, Rb anti-human - 60-90 min (same I use for IHC)
6. Wash TBST -5-10min
7. secondary Donkey anti-Rb 594 red - 60min
8. Wash TBST -5-10min, H2O -5min
9. DAPI - 10min
10. Wash H2O -5-10min
11. Gelvatol coverslip
Am I using wrong secondary? Is there any specific secondary or protocol
for IF staining for FFPE to avoid background? I just used same AR and
primary Ab's dilution like i use for IHC with nice results.
Any suggestions are appreciated, especially in these Holidays days from
working histo- people:)
Thanks and have a warm and nice Holidays,
Naira
------------------------------
Message: 5
Date: Thu, 24 Dec 2009 09:03:58 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: [Histonet] Hello fellow Histo's , 2 questions for you
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFEF9E5F26.5F9AD0CB-ON86257696.005209ED-86257696.0052C2D7 <@t> bhcpns.org>
Content-Type: text/plain;charset="US-ASCII"
First of all I hope all of you are enjoying your Holiday. And I expect
that many of you are not at work like me, but when you get a chance if
you
could help me out by answering these two questions I would be very
grateful. :-)
1) In your lab when you receive renal biopsy's fresh. Who finds the
Glomeruli using a microscope? A pathologist or a Histologist?
2) Also, if you have frozen sections off site, do you always send a
Histologist to assist the Pathologist?
Thanks for your help in advance
Many Blessing to each of you!
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
-----------------------------------------
All electronic data transmissions originating from or sent to
Baptist Health Care Corporation (BHC) are subject to monitoring.
This message along with any attached data, are the confidential and
proprietary communications of BHC and are intended to be received
only by the individual or individuals to whom the message has been
addressed. If the reader of this message is not the intended
recipient, please take notice that any use, copying, printing,
forwarding or distribution of this message, in any form, is
strictly prohibited and may violate State or Federal Law. If you
have received this transmission in error, please delete or destroy
all copies of this message. For questions, contact the BHC Privacy
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------------------------------
Message: 6
Date: Thu, 24 Dec 2009 07:18:31 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: [Histonet] Hello fellow Histo's , 2 questions for you
To: histonet <@t> lists.utsouthwestern.edu, Kim.Donadio <@t> bhcpns.org
Message-ID: <404178.71779.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Kim:
Although histology is practices very similarly all around the world,
each histolab operates with its own rules because there are no general
rules.
1- who finds the glomerulii? Usually is a pathologist, but some have
trained a histology technologist that takes care of the DIF or to
prepare the samples for TEM and the pathologist task is delegated to
that histotech. So, it depends on the pathology director to define how
the work is done.
2- off site frozen sections?ásometimes a histotech is sent to help the
pathologist, but usually the pathologist takes care of the whole thing.
So, again, it depends on the site although the custom is:
1- glomerulio are identified by the PT, and
2- PT take care of off site frozens.
Hope this will help you
Happy Holidays
RenΘ J.
--- On Thu, 12/24/09, Kim.Donadio <@t> bhcpns.org <Kim.Donadio <@t> bhcpns.org>
wrote:
From: Kim.Donadio <@t> bhcpns.org <Kim.Donadio <@t> bhcpns.org>
Subject: [Histonet] Hello fellow Histo's , 2 questions for you
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, December 24, 2009, 10:03 AM
First of all I hope all of you are enjoying your Holiday. And I expect
that many of you are not at work like me, but when you get a chance if
you
could help me out by answering these two questions I would be very
grateful. :-)
1) In your lab when you receive renal biopsy'sá fresh. Who finds the
Glomeruli using a microscope? A pathologist or a Histologist?
2) Also, if you have frozen sections off site, do you always send a
Histologist to assist the Pathologist?
Thanks for your help in advance
Many Blessing to each of you!
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
-----------------------------------------
All electronic data transmissions originating from or sent to
Baptist Health Care Corporation (BHC) are subject to monitoring.
This message along with any attached data, are the confidential and
proprietary communications of BHC and are intended to be received
only by the individual or individuals to whom the message has been
addressed. If the reader of this message is not the intended
recipient, please take notice that any use, copying, printing,
forwarding or distribution of this message, in any form, is
strictly prohibited and may violate State or Federal Law. If you
have received this transmission in error, please delete or destroy
all copies of this message.á For questions, contact the BHC Privacy
Officer at (850) 434-4472.á Rev.10/07.
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