[Histonet] leaving IHC slides in wash buffer

Jennifer Campbell jcampbell <@t> vdxpathology.com
Wed Dec 16 12:34:40 CST 2009


Hi Everyone,
 
  I have yet another IHC-related question.  So after antigen retrieval,
and then allowing my slides too cool, I place them in wash buffer before
loading them on to the autostainer.  Would leaving the slides in the
wash buffer in the fridge for an extended period of time (say, 1 week)
before continuing with immunostaining affect staining quality in any
way?
 
Thank you,
 
Jen Campbell


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