SPAM-LOW: [Histonet] zinc or alcoholic formalin

Patsy Ruegg pruegg <@t> ihctech.net
Sun Dec 6 15:48:46 CST 2009


I use zinc formalin and my process is the same as with formalin, I have
heard some people complain that zinc formalin can precipitate in the tissue
processor but I have not ever seen that, I have been using zinc formalin for
15 years with no problems.  I process a lot of animal tissue so my
processing schedule is shorter than for human tissue, for animal I spend
only 20 min per station rather than the hour I spend for human tissues.
Animal tissue has less fat and can be over processed (it will get hard and
dry) easily.

Regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chris
Evanish
Sent: Friday, November 20, 2009 11:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] zinc or alcoholic formalin

Does anyone use zinc formalin or alcoholic formalin on their tissue
processors? If so what is your routine run program

 Chris
Montgomery Hospital

>>> <histonet-request <@t> lists.utsouthwestern.edu> 11/20/2009 1:05 PM >>>
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Today's Topics:

   1. Second Call for Manuscripts for Special Issue of Journal	of
      Histotechnology (gayle callis)
   2. Preventing slide labeling mistakes (Morken, Tim)
   3. Re: Preventing slide labeling mistakes (Lynette Pavelich)
   4. Re: Fwd: foxp3 ab ihc (Marilyn Tyler)
   5. RE: Preventing slide labeling mistakes (Mike Pence)
   6. RE: SPAM-LOW:  [Histonet] Re: freezing skeletal muscle
      (Patsy Ruegg)
   7. Re: Preventing slide labeling mistakes (Victor Tobias)
   8. RE: Preventing slide labeling mistakes (Morken, Tim)
   9. RE: Preventing slide labeling mistakes (Sebree Linda A)
  10. RE: Preventing slide labeling mistakes (Armandi, Arlene)
  11. CAP guidelines for positive controls (Tiana Fountain)
  12. CAP QUESTION ANP.12425 ASR disclaimer on pathology	reports
      (Foshey, Annette)
  13. course recommendation (Mike Tighe)
  14. Re: CAP QUESTION ANP.12425 ASR disclaimer on pathology
      reports (Rene J Buesa)
  15. AW: [Histonet] Preventing slide labeling mistakes (Gudrun Lang)


----------------------------------------------------------------------

Message: 1
Date: Thu, 19 Nov 2009 16:11:37 -0700
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Second Call for Manuscripts for Special Issue of
	Journal	of Histotechnology
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002301ca696d$a65f0fc0$f31d2f40$@callis <@t> bresnan.net>
Content-Type: text/plain;	charset="utf-8"

Dear Histonet Colleagues, 

 

A second call for manuscripts for Special Issue of Journal of
Histotechnology: 

 

The Journal of Histotechnology (JOH) editors, Editorial Board, and
guest editor Dr. Richard Cartun are pleased to announce a call for
manuscripts for the March 2010 special issue of JOH, with focus on
Immunohistochemistry and In Situ Hybridization.  Technical notes and
methodology-focused contributions are particularly encouraged; clinical,
research, and veterinary topics are welcome.  Manuscripts must be
submitted prior to November 30, 2009 in order to be considered for the
March issue.  Manuscripts may be sent to Ms. Jane Jacobi, Managing
Editor, at  <mailto:joh <@t> nsh.org> joh <@t> nsh.org.  Authors wishing
pre-submission guidance with the writing process and assignment of a
writing partner may contact Ms. Gayle Callis, JOH Assistant Editor, at 
<mailto:gayle.callis <@t> bresnan.net> gayle.callis <@t> bresnan.net.   
Information for authors is detailed at  <http://www.nsh.org/>
www.nsh.org under Journal of Histotechnology.  and an example of a
Technical Note format is available upon request from Ms. Callis and NSH
office.    

 

Gayle M. Callis

JOH Assistant Editor

National Society for Histotechnology

Journal of Histotechnology

10320 Little Patuxent Pkwy #804

Columbia, MD 21044

P: 443-535-4060

E: joh <@t> nsh.org 

 



------------------------------

Message: 2
Date: Thu, 19 Nov 2009 16:20:16 -0800
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] Preventing slide labeling mistakes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<1AAF670737F193429070841C6B2ADD4CF7892093 <@t> EXMBMCB15.ucsfmedicalcenter.org>
	
Content-Type: text/plain; charset=us-ascii

Hi, Can people share their procedures for preventing manual slide
labeling mistakes? No need to include barcoding - we are exploring that
but it is a ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time), require labeling slides (hand-written) only at the time of
cutting a single block, and matching paper label to the slide after
staining. We don't currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA



------------------------------

Message: 3
Date: Thu, 19 Nov 2009 22:52:07 -0500
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Subject: Re: [Histonet] Preventing slide labeling mistakes
To: <histonet <@t> lists.utsouthwestern.edu>,
	<Timothy.Morken <@t> ucsfmedctr.org>
Message-ID: <4B05CC17020000EE0002E9B9 <@t> smtp-gw.hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

Hi Tim,
We do everything you do, but we also match our blocks to the written
number on the slides.  We catch the majority of our mistakes at that
point.  The other is because the font on the label is so small, we
have
a hard time seeing the difference between 5's & 6's!!!  I'm sure it's
not our ages or anything!   We're working with CoPath to get a larger
font!!  Ahhh, getting old is SO fun!  And yes, reading glasses work
pretty good!! LOL

Have a great weekend everyone!!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com 
ph:  810-257-9948
fax:  810-762-7082
>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 11/19/09 7:20 PM >>>
Hi, Can people share their procedures for preventing manual slide
labeling mistakes? No need to include barcoding - we are exploring
that
but it is a ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time), require labeling slides (hand-written) only at the time of
cutting a single block, and matching paper label to the slide after
staining. We don't currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 4
Date: Fri, 20 Nov 2009 07:52:05 +0200
From: "Marilyn Tyler" <Marilyn.Tyler <@t> uct.ac.za>
Subject: Re: [Histonet] Fwd: foxp3 ab ihc
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4B064AA5020000900008096B <@t> gwiasmtp.uct.ac.za>
Content-Type: text/plain; charset=US-ASCII

Thanks to all in Histonet world posted on Heathers behalf who is still
having problems with her access
Marilyn

>>> Heather McCleod 2009/11/19 10:43 AM >>>
Sorry to bother you Marilyn.  Please forward. I have an answer, i.e.
confirmation of what I would like to use.
 
Thanks to all who replied to my foxp3 query.
Heather

>>> "Marilyn Tyler" <Marilyn.Tyler <@t> uct.ac.za> 2009/11/18 09:47 AM >>>

Does anybody in histoland use any FOXP3 antibody.  I would like to know
which is the better ab to use for FFPE human material.
Posting on behalf of Heather as she cannot get through to Histonet
>>> Heather McCleod 2009/11/18 08:37 AM >>>


Thank you
The Histonet ROCKS!!!
Heather
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


------------------------------

Message: 5
Date: Fri, 20 Nov 2009 08:37:53 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Preventing slide labeling mistakes
To: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>,
	<histonet <@t> lists.utsouthwestern.edu>,
<Timothy.Morken <@t> ucsfmedctr.org>
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A3CA2 <@t> is-e2k3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

You must match your blocks and slides. This will eliminate a lot of
your
mistakes/ NOT ALL!

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lynette
Pavelich
Sent: Thursday, November 19, 2009 9:52 PM
To: histonet <@t> lists.utsouthwestern.edu; Timothy.Morken <@t> ucsfmedctr.org 
Subject: Re: [Histonet] Preventing slide labeling mistakes


Hi Tim,
We do everything you do, but we also match our blocks to the written
number on the slides.  We catch the majority of our mistakes at that
point.  The other is because the font on the label is so small, we
have
a hard time seeing the difference between 5's & 6's!!!  I'm sure it's
not our ages or anything!   We're working with CoPath to get a larger
font!!  Ahhh, getting old is SO fun!  And yes, reading glasses work
pretty good!! LOL

Have a great weekend everyone!!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com 
ph:  810-257-9948
fax:  810-762-7082
>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 11/19/09 7:20 PM >>>
Hi, Can people share their procedures for preventing manual slide
labeling mistakes? No need to include barcoding - we are exploring
that
but it is a ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time), require labeling slides (hand-written) only at the time of
cutting a single block, and matching paper label to the slide after
staining. We don't currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 





------------------------------

Message: 6
Date: Fri, 20 Nov 2009 07:52:54 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW:  [Histonet] Re: freezing skeletal muscle
To: "'Robert Richmond'" <rsrichmond <@t> gmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CB5F4B5ED5D248B389186EB2A4CEDD8F <@t> prueggihctechlt>
Content-Type: text/plain;	charset="us-ascii"

Especially for bone, I have found that formalin fixation and then
infiltrating in 30% sucrose before freezing the way Samuri described
works
the very best for me.

Regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Thursday, November 19, 2009 11:22 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: SPAM-LOW: [Histonet] Re: freezing skeletal muscle

Galina Deyneko asks about freezing skeletal muscle.

I'm not sure that temperature control of the isopentane is too
important - it should be very slightly viscous. But do get rid of
explosive isopentane and get a non-flammable substitute - see my
previous posts on this topic.

The technique for freezing skeletal muscle is easier demonstrated than
described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat
it with talc powder so that it appears white on the surface. Then dip
the specimen in and out of the isopentane - roughly two dips per
second - until it is frozen solid. That will eliminate the ice crystal
artifact. But you have to try it a few times before it will quite work
for you. Set aside one dead rat and one afternoon.

Bob Richmond
Samurai Pathologist
Knoxville TN

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 





------------------------------

Message: 7
Date: Fri, 20 Nov 2009 07:10:36 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] Preventing slide labeling mistakes
To: Lynette Pavelich <lpaveli1 <@t> hurleymc.com>
Cc: histonet <@t> lists.utsouthwestern.edu, Timothy.Morken <@t> ucsfmedctr.org 
Message-ID: <4B06B16C.2000507 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Lynette,

You might want to also investigate different fonts if that is an
option. 
Keeping the same size but using a different font can make a world of 
difference.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu 
206-598-2792
206-598-7659 Fax
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then destroy all copies of the message and any attachments.



Lynette Pavelich wrote:
> Hi Tim,
> We do everything you do, but we also match our blocks to the written
> number on the slides.  We catch the majority of our mistakes at that
> point.  The other is because the font on the label is so small, we
have
> a hard time seeing the difference between 5's & 6's!!!  I'm sure
it's
> not our ages or anything!   We're working with CoPath to get a
larger
> font!!  Ahhh, getting old is SO fun!  And yes, reading glasses work
> pretty good!! LOL
>
> Have a great weekend everyone!!
> Lynette
>
> Lynette Pavelich, HT(ASCP)
> Histology Supervisor
> MSH Competency Coordinator
> Hurley Medical Center
> One Hurley Plaza
> Flint, MI  48503
> email: Lpaveli1 <@t> hurleymc.com 
> ph:  810-257-9948
> fax:  810-762-7082
>   
>>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 11/19/09 7:20 PM
>>>
>>>>         
> Hi, Can people share their procedures for preventing manual slide
> labeling mistakes? No need to include barcoding - we are exploring
that
> but it is a ways off.
>
> We currently have a manual process:
> We prohibit pre-labeling slides in batches (many blocks/slides at
one
> time), require labeling slides (hand-written) only at the time of
> cutting a single block, and matching paper label to the slide after
> staining. We don't currently match blocks to slides.
>
> Thanks for any info!
>
> Tim Morken
> Supervisor, Histology / IPOX
> UCSF Medical Center
> San Francisco, CA
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>   



------------------------------

Message: 8
Date: Fri, 20 Nov 2009 08:41:47 -0800
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Preventing slide labeling mistakes
To: "Mike Pence" <mpence <@t> grhs.net>, "Lynette Pavelich"
	<lpaveli1 <@t> hurleymc.com>,	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<1AAF670737F193429070841C6B2ADD4CF7892156 <@t> EXMBMCB15.ucsfmedicalcenter.org>
	
Content-Type: text/plain; charset=us-ascii

Thanks for the suggestions. One clarification: matching blocks and
slides CATCHES mistakes; it does not reduce or eliminate them. 

Tim 
 

-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net] 
Sent: Friday, November 20, 2009 6:38 AM
To: Lynette Pavelich; histonet <@t> lists.utsouthwestern.edu; Morken, Tim
Subject: RE: [Histonet] Preventing slide labeling mistakes

You must match your blocks and slides. This will eliminate a lot of
your
mistakes/ NOT ALL!

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lynette
Pavelich
Sent: Thursday, November 19, 2009 9:52 PM
To: histonet <@t> lists.utsouthwestern.edu; Timothy.Morken <@t> ucsfmedctr.org 
Subject: Re: [Histonet] Preventing slide labeling mistakes


Hi Tim,
We do everything you do, but we also match our blocks to the written
number on the slides.  We catch the majority of our mistakes at that
point.  The other is because the font on the label is so small, we
have
a hard time seeing the difference between 5's & 6's!!!  I'm sure it's
not our ages or anything!   We're working with CoPath to get a larger
font!!  Ahhh, getting old is SO fun!  And yes, reading glasses work
pretty good!! LOL

Have a great weekend everyone!!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com 
ph:  810-257-9948
fax:  810-762-7082
>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 11/19/09 7:20 PM >>>
Hi, Can people share their procedures for preventing manual slide
labeling mistakes? No need to include barcoding - we are exploring
that
but it is a ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time), require labeling slides (hand-written) only at the time of
cutting a single block, and matching paper label to the slide after
staining. We don't currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






------------------------------

Message: 9
Date: Fri, 20 Nov 2009 10:55:04 -0600
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Preventing slide labeling mistakes
To: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>,	"Mike Pence"
	<mpence <@t> grhs.net>, "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<8C023B4AB999614BA4791BAEB26E27381BF677 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="us-ascii"

If the matching is done at the time of sectioning, i.e. making sure
the
block you are about to cut matches the slide you have ready to pick up
that block's section(s), it will reduce mislabeling errors....and its
GLP!

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Morken,
Tim
Sent: Friday, November 20, 2009 10:42 AM
To: Mike Pence; Lynette Pavelich; histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] Preventing slide labeling mistakes


Thanks for the suggestions. One clarification: matching blocks and
slides CATCHES mistakes; it does not reduce or eliminate them. 

Tim 
 

-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net] 
Sent: Friday, November 20, 2009 6:38 AM
To: Lynette Pavelich; histonet <@t> lists.utsouthwestern.edu; Morken, Tim
Subject: RE: [Histonet] Preventing slide labeling mistakes

You must match your blocks and slides. This will eliminate a lot of
your
mistakes/ NOT ALL!

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lynette
Pavelich
Sent: Thursday, November 19, 2009 9:52 PM
To: histonet <@t> lists.utsouthwestern.edu; Timothy.Morken <@t> ucsfmedctr.org 
Subject: Re: [Histonet] Preventing slide labeling mistakes


Hi Tim,
We do everything you do, but we also match our blocks to the written
number on the slides.  We catch the majority of our mistakes at that
point.  The other is because the font on the label is so small, we
have
a hard time seeing the difference between 5's & 6's!!!  I'm sure it's
not our ages or anything!   We're working with CoPath to get a larger
font!!  Ahhh, getting old is SO fun!  And yes, reading glasses work
pretty good!! LOL

Have a great weekend everyone!!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com 
ph:  810-257-9948
fax:  810-762-7082
>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 11/19/09 7:20 PM >>>
Hi, Can people share their procedures for preventing manual slide
labeling mistakes? No need to include barcoding - we are exploring
that
but it is a ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time), require labeling slides (hand-written) only at the time of
cutting a single block, and matching paper label to the slide after
staining. We don't currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



------------------------------

Message: 10
Date: Fri, 20 Nov 2009 09:03:08 -0800
From: "Armandi, Arlene" <Arlene.Armandi <@t> cshs.org>
Subject: RE: [Histonet] Preventing slide labeling mistakes
To: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>,	"Mike Pence"
	<mpence <@t> grhs.net>, "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CC5439C5EAECF04C970324A65854D11D0AB62391 <@t> EXVS4.CSMC.EDU>
Content-Type: text/plain;	charset="US-ASCII"

One more suggestion. Have you considered using Xylene resistant slide
labels? We print the labels at the time we put the blocks in order. We
then put the corresponding labels on the trays with the blocks. When
the
tech picks up a tray, they have the labels ready to put directly onto
the slides as they cut.
This eliminates the labeling errors resulting from the matching the
hand
written slides (sometimes illegible) to the labels, after the slides
have been stained. Just a thought.

Arlene

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Morken,
Tim
Sent: Friday, November 20, 2009 8:42 AM
To: Mike Pence; Lynette Pavelich; histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] Preventing slide labeling mistakes

Thanks for the suggestions. One clarification: matching blocks and
slides CATCHES mistakes; it does not reduce or eliminate them. 

Tim 
 

-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net] 
Sent: Friday, November 20, 2009 6:38 AM
To: Lynette Pavelich; histonet <@t> lists.utsouthwestern.edu; Morken, Tim
Subject: RE: [Histonet] Preventing slide labeling mistakes

You must match your blocks and slides. This will eliminate a lot of
your
mistakes/ NOT ALL!

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lynette
Pavelich
Sent: Thursday, November 19, 2009 9:52 PM
To: histonet <@t> lists.utsouthwestern.edu; Timothy.Morken <@t> ucsfmedctr.org 
Subject: Re: [Histonet] Preventing slide labeling mistakes


Hi Tim,
We do everything you do, but we also match our blocks to the written
number on the slides.  We catch the majority of our mistakes at that
point.  The other is because the font on the label is so small, we
have
a hard time seeing the difference between 5's & 6's!!!  I'm sure it's
not our ages or anything!   We're working with CoPath to get a larger
font!!  Ahhh, getting old is SO fun!  And yes, reading glasses work
pretty good!! LOL

Have a great weekend everyone!!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com 
ph:  810-257-9948
fax:  810-762-7082
>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 11/19/09 7:20 PM >>>
Hi, Can people share their procedures for preventing manual slide
labeling mistakes? No need to include barcoding - we are exploring
that
but it is a ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time), require labeling slides (hand-written) only at the time of
cutting a single block, and matching paper label to the slide after
staining. We don't currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

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Message: 11
Date: Fri, 20 Nov 2009 11:14:23 -0600
From: "Tiana Fountain" <tfountain <@t> exchange.hsc.mb.ca>
Subject: [Histonet] CAP guidelines for positive controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<BF2F7B1BBD30C5478EF41CF80164D99C7A7D6F <@t> hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="us-ascii"

Hello,

I am trying to find the guidelines for CAP that describes how long IHC
positive control slides are to be kept on file ... does anyone know? 

Thanks in advance!

Tiana
 

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Message: 12
Date: Fri, 20 Nov 2009 11:54:59 -0600
From: "Foshey, Annette" <AFoshey <@t> chw.org>
Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology
	reports
To: " (histonet <@t> lists.utsouthwestern.edu)" 
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<F6F723A7AC0E3646A3E4453ED181A87337CA3FB42C <@t> C1EXCPWV02.chwi.chswi.org>
Content-Type: text/plain; charset="us-ascii"

What is the current practice for meeting this regulation?  Is a general
statement (disclaimer) placed on all pathology reports that have
immunocytochemistry and/or
FISH and ISH or is the disclaimer placed only on the reports where the
class 1 ASR antibody or nucleic acid have been used?

Thanks for your input?
Annette Foshey
Charge Tech in Histology
Children's Hospital of Wisconsin
414-266-6580        Fax 414-266-2779
afoshey <@t> chw.org 




------------------------------

Message: 13
Date: Fri, 20 Nov 2009 12:57:36 -0500
From: "Mike Tighe" <mtighe <@t> trudeauinstitute.org>
Subject: [Histonet] course recommendation
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4B069246.26E4.00EE.0 <@t> trudeauinstitute.org>
Content-Type: text/plain; charset=US-ASCII


I am looking for a conference/course to attend in 2010. I am
experienced in IF and IHC and would like to learn more about any new
techniques and products that might be available. Does anyone have a
recommendation for a course or a conference in the US? I am not a
certified HT but I pretend to be one:)

Thanks for any suggestions!!
Mike




------------------------------

Message: 14
Date: Fri, 20 Nov 2009 09:58:17 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on
	pathology	reports
To: "\(histonet <@t> lists.utsouthwestern.edu\)"
	<histonet <@t> lists.utsouthwestern.edu>, AnnetteFoshey
<AFoshey <@t> chw.org>
Message-ID: <654236.83215.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

We used to add that the methods were experimental.
René J.

--- On Fri, 11/20/09, Foshey, Annette <AFoshey <@t> chw.org> wrote:


From: Foshey, Annette <AFoshey <@t> chw.org>
Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology
reports
To: " (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Date: Friday, November 20, 2009, 12:54 PM


What is the current practice for meeting this regulation?  Is a general
statement (disclaimer) placed on all pathology reports that have
immunocytochemistry and/or
FISH and ISH or is the disclaimer placed only on the reports where the
class 1 ASR antibody or nucleic acid have been used?

Thanks for your input?
Annette Foshey
Charge Tech in Histology
Children's Hospital of Wisconsin
414-266-6580        Fax 414-266-2779
afoshey <@t> chw.org 


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------------------------------

Message: 15
Date: Fri, 20 Nov 2009 18:59:49 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Preventing slide labeling mistakes
To: "'Morken, Tim'" <Timothy.Morken <@t> ucsfmedctr.org>
Cc: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <BA14DE0B587F429EAAE1808F03B8C72B <@t> dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

We write a "macro-protocol" at the time of grossing with all
blocknumbers
and additional identifer.
This paper is matched the first time with all blocks after embedding
and
before cutting. Missing blocks or wrong labelled blocks are found. 
The second time the paper is matched with all slides after staining
and
before delivering. Missing and wrong labelled slides are found.
Additional
the slide-number and material is matched with the "case sheet".

The slides are labelled batchwise with 10 per batch immediatly before
cutting.
The identifiers (letters, "lateral", "resection-margin", etc.) are
written
on side of the cassette, so numbers can be written big enough. The
numbers
have maximum of 5 digits slash year. 12345/09

Gudrun



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
Morken,
Tim
Gesendet: Freitag, 20. November 2009 01:20
An: histonet <@t> lists.utsouthwestern.edu 
Betreff: [Histonet] Preventing slide labeling mistakes

Hi, Can people share their procedures for preventing manual slide
labeling
mistakes? No need to include barcoding - we are exploring that but it
is a
ways off.

We currently have a manual process:
We prohibit pre-labeling slides in batches (many blocks/slides at one
time),
require labeling slides (hand-written) only at the time of cutting a
single
block, and matching paper label to the slide after staining. We don't
currently match blocks to slides.

Thanks for any info!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

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