[Histonet] procedure for osmium and potassium chromate?

[Cynthia Pyse]

OSMIUM TETROXIDE FOR FAT (Paraffin SECTIONS)

Solutions
2.5% Potassium Dichromate Solution Stock
1% Osmium Tetroxide Solution Stock

Potassium Dichromate-Osmium Tetroxide Solution Working
  Potassium Dichromate Stock   50 ml
  Osmium Tetroxide stock     50 ml

Staining Procedure
1. Place formalin fixed specimen, no thicker than 4mm, in working potassium
dichromate-osmium tetroxide solution for up to 16 hours.
2. Wash specimen in running water for 2 hours.
3. Process tissue, embed in paraffin and cut at 4 microns.
4. Stain in routine H&E staining setup, than mount.
Results:
  Fat - black
  Background - same as H&E stain.
Hope this helps. If you have any questions please let me know.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, December 02, 2009 3:13 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] procedure for osmium and potassium chromate?

I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dichromate, does anyone out there have this
protocol available.  I have found it before on the web but for some
reason I can't  find it this time.

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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